LOCUS       DAGCWU010000001        55598 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.001, whole genome shotgun sequence.
ACCESSION   DAGCWU010000001 DAGCWU010000000
VERSION     DAGCWU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.001"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            100..1164
                     /locus_tag="MBZ71_000001"
     CDS             100..1164
                     /locus_tag="MBZ71_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBT4427985.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1410..1835
                     /locus_tag="MBZ71_000002"
     CDS             1410..1835
                     /locus_tag="MBZ71_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4427986.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1828..2277
                     /gene="nusB"
                     /locus_tag="MBZ71_000003"
     CDS             1828..2277
                     /gene="nusB"
                     /locus_tag="MBZ71_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBT4427987.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2374..3228
                     /locus_tag="MBZ71_000004"
     CDS             2374..3228
                     /locus_tag="MBZ71_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBT4427988.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3229..4566
                     /locus_tag="MBZ71_000005"
     CDS             3229..4566
                     /locus_tag="MBZ71_000005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBT4427989.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4571..4795
                     /locus_tag="MBZ71_000006"
     CDS             4571..4795
                     /locus_tag="MBZ71_000006"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBT4427990.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4797..5687
                     /locus_tag="MBZ71_000007"
     CDS             4797..5687
                     /locus_tag="MBZ71_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBT4427991.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5696..6514
                     /locus_tag="MBZ71_000008"
     CDS             5696..6514
                     /locus_tag="MBZ71_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBT4427992.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6621..7070
                     /locus_tag="MBZ71_000009"
     CDS             6621..7070
                     /locus_tag="MBZ71_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBT4427993.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7088..8770
                     /gene="recN"
                     /locus_tag="MBZ71_000010"
     CDS             7088..8770
                     /gene="recN"
                     /locus_tag="MBZ71_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBT4427994.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8825..8941)
                     /locus_tag="MBZ71_000011"
     CDS             complement(8825..8941)
                     /locus_tag="MBZ71_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBT4427995.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9014..9970)
                     /locus_tag="MBZ71_000012"
     CDS             complement(9014..9970)
                     /locus_tag="MBZ71_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBT4427996.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10141..10509
                     /locus_tag="MBZ71_000013"
     CDS             10141..10509
                     /locus_tag="MBZ71_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBT4427997.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10679..11110
                     /gene="mraZ"
                     /locus_tag="MBZ71_000014"
     CDS             10679..11110
                     /gene="mraZ"
                     /locus_tag="MBZ71_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBT4427998.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11131..12090
                     /gene="rsmH"
                     /locus_tag="MBZ71_000015"
     CDS             11131..12090
                     /gene="rsmH"
                     /locus_tag="MBZ71_000015"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBT4427999.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12095..12496
                     /gene="ftsL"
                     /locus_tag="MBZ71_000016"
     CDS             12095..12496
                     /gene="ftsL"
                     /locus_tag="MBZ71_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBT4428000.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12497..14689
                     /locus_tag="MBZ71_000017"
     CDS             12497..14689
                     /locus_tag="MBZ71_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBT4428001.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14715..15677
                     /locus_tag="MBZ71_000018"
     CDS             14715..15677
                     /locus_tag="MBZ71_000018"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBT4428002.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15691..17052
                     /locus_tag="MBZ71_000019"
     CDS             15691..17052
                     /locus_tag="MBZ71_000019"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBT4428003.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17078..18166
                     /gene="murG"
                     /locus_tag="MBZ71_000020"
     CDS             17078..18166
                     /gene="murG"
                     /locus_tag="MBZ71_000020"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBT4428004.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18181..19401
                     /locus_tag="MBZ71_000021"
     CDS             18181..19401
                     /locus_tag="MBZ71_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBT4428005.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19557..20882
                     /gene="ftsA"
                     /locus_tag="MBZ71_000022"
     CDS             19557..20882
                     /gene="ftsA"
                     /locus_tag="MBZ71_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBT4428006.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20905..22146
                     /gene="ftsZ"
                     /locus_tag="MBZ71_000023"
     CDS             20905..22146
                     /gene="ftsZ"
                     /locus_tag="MBZ71_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBT4428007.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22163..22840
                     /locus_tag="MBZ71_000024"
     CDS             22163..22840
                     /locus_tag="MBZ71_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBT4428008.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22854..23453
                     /locus_tag="MBZ71_000025"
     CDS             22854..23453
                     /locus_tag="MBZ71_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBT4428009.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23468..23755
                     /locus_tag="MBZ71_000026"
     CDS             23468..23755
                     /locus_tag="MBZ71_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBT4428010.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23804..24586
                     /locus_tag="MBZ71_000027"
     CDS             23804..24586
                     /locus_tag="MBZ71_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBT4428011.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24607..25314
                     /locus_tag="MBZ71_000028"
     CDS             24607..25314
                     /locus_tag="MBZ71_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBT4428012.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25576..28374
                     /gene="ileS"
                     /locus_tag="MBZ71_000029"
     CDS             25576..28374
                     /gene="ileS"
                     /locus_tag="MBZ71_000029"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBT4428013.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28423..29949)
                     /locus_tag="MBZ71_000030"
     CDS             complement(28423..29949)
                     /locus_tag="MBZ71_000030"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBT4428014.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(29964..30611)
                     /locus_tag="MBZ71_000031"
     CDS             complement(29964..30611)
                     /locus_tag="MBZ71_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBT4428015.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30795..31124)
                     /locus_tag="MBZ71_000032"
     CDS             complement(30795..31124)
                     /locus_tag="MBZ71_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBT4428016.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31301..32029
                     /gene="sfsA"
                     /locus_tag="MBZ71_000033"
     CDS             31301..32029
                     /gene="sfsA"
                     /locus_tag="MBZ71_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBT4428017.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32045..33058
                     /locus_tag="MBZ71_000034"
     CDS             32045..33058
                     /locus_tag="MBZ71_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4428018.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33058..34335
                     /locus_tag="MBZ71_000035"
     CDS             33058..34335
                     /locus_tag="MBZ71_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBT4428019.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34398..37100
                     /locus_tag="MBZ71_000036"
     CDS             34398..37100
                     /locus_tag="MBZ71_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4428020.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37252..37569
                     /locus_tag="MBZ71_000037"
     CDS             37252..37569
                     /locus_tag="MBZ71_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4428021.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37599..37919
                     /locus_tag="MBZ71_000038"
     CDS             37599..37919
                     /locus_tag="MBZ71_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBT4428022.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38027..39487
                     /locus_tag="MBZ71_000039"
     CDS             38027..39487
                     /locus_tag="MBZ71_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4428023.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39555..39776
                     /locus_tag="MBZ71_000040"
     CDS             39555..39776
                     /locus_tag="MBZ71_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428024.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39807..39989
                     /locus_tag="MBZ71_000041"
     CDS             39807..39989
                     /locus_tag="MBZ71_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBT4428025.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            39989..40402
                     /locus_tag="MBZ71_000042"
     CDS             39989..40402
                     /locus_tag="MBZ71_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBT4428026.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40525..41706
                     /locus_tag="MBZ71_000043"
     CDS             40525..41706
                     /locus_tag="MBZ71_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4428027.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAELGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(41777..41941)
                     /locus_tag="MBZ71_000044"
     CDS             complement(41777..41941)
                     /locus_tag="MBZ71_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428028.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            42095..42168
                     /locus_tag="MBZ71_000045"
     tRNA            42095..42168
                     /locus_tag="MBZ71_000045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42129..42131,aa:Arg,seq:cct)
     gene            complement(42237..43376)
                     /locus_tag="MBZ71_000046"
     CDS             complement(42237..43376)
                     /locus_tag="MBZ71_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4428029.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(43492..44127)
                     /locus_tag="MBZ71_000047"
     CDS             complement(43492..44127)
                     /locus_tag="MBZ71_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBT4428030.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLILLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTAKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(44210..44632)
                     /locus_tag="MBZ71_000048"
     CDS             complement(44210..44632)
                     /locus_tag="MBZ71_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBT4428031.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(44650..44970)
                     /locus_tag="MBZ71_000049"
     CDS             complement(44650..44970)
                     /locus_tag="MBZ71_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4428032.1"
                     /translation="MNPYEKIKELTKQNGISVRELEKRLGYSNGYFSKWKSVSPNSEG
                     LAKVSDYFGVSIDYLLGRDKKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            45271..46047
                     /locus_tag="MBZ71_000050"
     CDS             45271..46047
                     /locus_tag="MBZ71_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBT4428033.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            46062..46265
                     /locus_tag="MBZ71_000051"
     CDS             46062..46265
                     /locus_tag="MBZ71_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4428034.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            46281..46619
                     /locus_tag="MBZ71_000052"
     CDS             46281..46619
                     /locus_tag="MBZ71_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBT4428035.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            46606..46776
                     /locus_tag="MBZ71_000053"
     CDS             46606..46776
                     /locus_tag="MBZ71_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428036.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYKTKKPVGRD"
     gene            complement(46874..47539)
                     /locus_tag="MBZ71_000054"
     CDS             complement(46874..47539)
                     /locus_tag="MBZ71_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428037.1"
                     /translation="MSLIDFIKQFDWNNIVKTIKDFILPITSLVFSTFAFYISYTDRK
                     NKKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTNNSSLPVTIIEFSI
                     SGIPDPLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGADLSKFPPLSLPITI
                     PPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQISQLDTGYAPPQLD
                     EFD"
     gene            47594..48292
                     /locus_tag="MBZ71_000055"
     CDS             47594..48292
                     /locus_tag="MBZ71_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBT4428038.1"
                     /translation="MQELVILKNKEAVTTSIQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNTKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            48470..48811
                     /locus_tag="MBZ71_000056"
     CDS             48470..48811
                     /locus_tag="MBZ71_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428039.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            48804..49475
                     /locus_tag="MBZ71_000057"
     CDS             48804..49475
                     /locus_tag="MBZ71_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBT4428040.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            49481..50167
                     /locus_tag="MBZ71_000058"
     CDS             49481..50167
                     /locus_tag="MBZ71_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428041.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            50174..51025
                     /locus_tag="MBZ71_000059"
     CDS             50174..51025
                     /locus_tag="MBZ71_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBT4428042.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            51037..51306
                     /locus_tag="MBZ71_000060"
     CDS             51037..51306
                     /locus_tag="MBZ71_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428043.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            51311..51475
                     /locus_tag="MBZ71_000061"
     CDS             51311..51475
                     /locus_tag="MBZ71_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428044.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            51468..51788
                     /locus_tag="MBZ71_000062"
     CDS             51468..51788
                     /locus_tag="MBZ71_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428045.1"
                     /translation="MDKKAAWRKLMLLIQDENWQEDEAVVAEVQRLEKIANGRIRKKP
                     DKRKLRKGKIIVVLYEGNILMQGTARELSAETEYTSGTIRTYAWRNHVDKKGHEYKYL
                     EGSK"
     gene            51785..52174
                     /locus_tag="MBZ71_000063"
     CDS             51785..52174
                     /locus_tag="MBZ71_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428046.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVGIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRV
                     FKHKKMDYEYEVIGNIYENSELLEEQR"
     gene            52171..52764
                     /locus_tag="MBZ71_000064"
     CDS             52171..52764
                     /locus_tag="MBZ71_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBT4428047.1"
                     /translation="MNKQETIKKLESIKAIGNDAIAACYNESMNAGITLMKKIDEPQK
                     HVMPKFFDKWAKQVLEKRDKFYAISLIARAGWGYGVDFELSENGLSSENKELLYWLVD
                     KCSDNYPNKKKAIEALLYGYEVEKEPLYHVKLKIPGVTYYLIQTFLMPGDTHMCFSAA
                     TERYGAKWKNTFTESEIKAIDERYWPFAVPVEEEAEG"
     gene            52761..53138
                     /locus_tag="MBZ71_000065"
     CDS             52761..53138
                     /locus_tag="MBZ71_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428048.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQQIVFEWLKLTAPTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKEQFEVLAAFAQWGLEQEEAE"
     gene            53135..53338
                     /locus_tag="MBZ71_000066"
     CDS             53135..53338
                     /locus_tag="MBZ71_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBT4428049.1"
                     /translation="MNNRHRRITKLRKQELNVLKAKFEKEYGVSAEEAYKVASQCVAD
                     ASNAIRKFGISILSDDRKWEDIE"
     gene            53335..53544
                     /locus_tag="MBZ71_000067"
     CDS             53335..53544
                     /locus_tag="MBZ71_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428050.1"
                     /translation="MKVYVVRFGNKFYRSDERSIGNNVLFLVDFLQNARWFDNLEEAN
                     QVSRRLGGLTQVYELVTIDHEGVER"
     gene            53541..53834
                     /locus_tag="MBZ71_000068"
     CDS             53541..53834
                     /locus_tag="MBZ71_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428051.1"
                     /translation="MKLKDGFYASSHGIGGLMLGMPTKNPKTRKKPKFKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            53864..54304
                     /locus_tag="MBZ71_000069"
     CDS             53864..54304
                     /locus_tag="MBZ71_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4428052.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            54379..54846
                     /locus_tag="MBZ71_000070"
     CDS             54379..54846
                     /locus_tag="MBZ71_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBT4428053.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGMSIYVTAQRNNTSEESVKAESNKIIIQFASSLELVAFK"
     gene            complement(55256..>55598)
                     /locus_tag="MBZ71_000071"
     CDS             complement(55256..>55598)
                     /locus_tag="MBZ71_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428054.1"
                     /translation="DDYRSDMIEYIELFAKNSNNNIIIQTGGATICGTPIDFDAEVKV
                     NPLIDAMMDSFAEFRSKKIDDIEKDDEQSLVVKSIFLKDVTIIGERTTNIPFLVVFAD
                     QISAISLGNLE"
BASE COUNT        18882 a         9279 c        11641 g        15796 t
ORIGIN      
        1 ataaaaagaa gttttccgga aatttttatt taactgtcgt gtacgaagaa aagtttgcta
       61 taatggaaaa aagcgtgcaa agaaagaggg agtttcatta tgatgctacg tgtagaaaaa
      121 ttgcgcaaaa aaatgcaaga agaaaatttg gattcatttt tggttaccag tccgtacaat
      181 cttcgttact taacgaactt tacaggtacg acaggattag ctgttattac tttggaaaaa
      241 gcatttttta ttactgattt tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt
      301 gaaattatta aaaatgtcgg acctattttt gatgaggtag caaacttagt acgcaaagaa
      361 gaactgcact cattagcttt tgaggaaaca acagtctcat ttttagaata ttcggtttta
      421 gaagaaatca tcgatgcgga attagtacct gtttcacaga tgatcgaaga actcagagaa
      481 gtgaaagatg aagaagaaat cgctattatc gaaaaagcat gtcatattgc ggatatggca
      541 tatgatcaca tcctgaaaat gatccaaccg ggcatgactg agattgaagt cgcaaatcaa
      601 ctagactttt atatgcgttc tcttggcgct tcaggtgttt cttttgatac gatcgttgct
      661 agcggattgc gttcggctat gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt
      721 gacttgatca cattggactt tggttgctat tacgaagggt acgtttccga tatgacacgt
      781 acattcgcta ttggtgatcc tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat
      841 gctcaattag cagttattga tgctgcaaaa ccaggaatga ctggtgtcca attagatgca
      901 gttgctagag attatatttc aaaacatggt tacggggaag catttggaca ttctacagga
      961 catgggatcg gtttagagat ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa
     1021 tttgtaccag gaaatgtgat cactgatgaa ccagggatct acttaccagg aatcggtgga
     1081 gtacgtattg aggatgactt gttgatcaca gaagatggaa atcgtgtgtt gactcgttca
     1141 ccaaaagaat tgatcatctt atagtttcat caatagaaga agtaagcgta aaaatcagtg
     1201 ttcacagact ttgttataga aaatcactcg atttaatgat acactataga agaactttta
     1261 agctgtaaga gataataaag ttgggtgttt tctccaattc atagcagttt gcagctgatc
     1321 ggcggcagtt ttcttaccac gtgatcacta tgccgcatca tagagaattc ggatgtatct
     1381 cgtgcttagg caaagggagg aataaataaa tggcagatga aaaaaatttg attttaaacg
     1441 caaaccaaga tttaggagag atcgtgatcg ctcctgaagt catcgaagta attatcggga
     1501 ttgcagcttc taaagtagaa ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca
     1561 ctgaattatt aggacgtgca gctcacggaa aaggtgttta cttagcaaac gaggaagatg
     1621 gcttgaaagt agatctttac tgttatctag aatatggtgt ttcagttcct aaagtagcaa
     1681 tggaaatgca ggaacgggtc agccagcaag tccttttcat gacggatatc gatttatctg
     1741 aagtcaatat ccatgtagta gctgttgttc ctgaaaaatt agaacaacca acaatcgaag
     1801 aactttttga agacgaagag gaagagaatg aataaagaat tatcaagaca cgagattcgg
     1861 gaaatggcat tgcaggcttt atttccatta gacttcaatg cagacctaac aaaagaagat
     1921 gcgattttca acgcaatcga attagaccac cgcgacatga tcaacgaaga tgaatcagag
     1981 tttgttcctg tttatcttga tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg
     2041 gataaagtga ttgaaaaaca tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg
     2101 gatttggtga tcttgcgtat cgcgattttt gaaatgactt atgtgacaga tgtaccagcg
     2161 gctgttgctt taaatgaagc aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt
     2221 aaattcgtca acggtgtttt atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc
     2281 ctagcttttt tctatgaatc atcataaacc tgttataaat cgggaaaatt ctatggtaga
     2341 atgaaaacac aaaaaatatc tgggagtggt tagatggcag aattgattaa cggaaaagaa
     2401 ttagctgaaa aaatgcaggc agagattgct gaaaaaatcc aaaaattgaa agataatggt
     2461 atccatcccg gattagtcgt tttattggta ggggaaaatc cagccagcca aatctatgta
     2521 agaaataaag aacgtgtagc aaaagaaatc ggaatccatt cattggtaga gcgttatcct
     2581 gagacgattt ctgaagacga actacttgca gaaattgaaa aatacaatca agatcctagt
     2641 tttcatggta tattggtcca attaccattg ccaaagcata tagatgaaga aaagatcctg
     2701 ttagctatcc gccctgaaaa agacgtagac ggttttcatc cgatgaattt aggcagactc
     2761 cttgcgggta aaccggacaa gattccttgt actccatacg gcatcatgaa aatgtttgaa
     2821 gcttattcta tcgatccggc gggtaaacga gcagtagtta tcggacgaag caatatcgtc
     2881 ggtaaaccga tggctcagct actcttgatg gcagatgcaa ctgttactgt tgctcattct
     2941 aaaaccgaaa atctggctga actggcacaa gaagcagata ttttagtcgt agcaatagga
     3001 cgtggacatt ttgtcacaaa agaattcgta aaaccaggtg ctgtagtgat agatgtaggg
     3061 atgaatcgtg atgctaacgg taagctgatc ggtgatgtga aatttgatga agtggagcct
     3121 attgcaggct ggatcactcc tgtgccaaag ggtgtagggc caatgactat cacgatgctg
     3181 atgtatcaaa ccgtaaaatc tgccgaagct tttggagcag gtgaatagat ggcacaagaa
     3241 tatttaactg tcactacatt aacgaagtat ttgaaaagaa aatttgatgc cgatccttat
     3301 ttagaacgcg tatatttaac aggcgaaata tcgaatttcc gtttacgtgc caatgcccat
     3361 caatatttca gcttaaaaga tgaccatgca aaaatatctg ctattatgtt caaaggagca
     3421 ttccaaaaat tacgattcca gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt
     3481 tcattatatg aagcgggagg ttcttatcaa atctatgtag agcacatgga gcctgatgga
     3541 ataggtgctt tatatcaaga attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt
     3601 tttcaagggc ctaaaaaaat gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt
     3661 ccaagcggag cggttatccg cgatatcatc acgacggtga aaagaagata tccaattgtc
     3721 caattggttt tgttcccaac agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg
     3781 aatatccaga gagtagaaga gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt
     3841 ggttcgattg aggatttatg gccgtttaat gaagagcggg ttgctcgggc gatttatcaa
     3901 gctaatacgc cggtgatttc atctgtagga catgaaacag ataccacgat cgctgattta
     3961 gtagcagatg ttcgggctgc cactccaaca gcagctgcag aattagctgt tcctgttttg
     4021 tcagaagagt tgatgcgcat tcaagaacgg caagctagat tgcagcaggc gtttgtacgc
     4081 cagatccagc gcaaacaaga acggttcgag cgtgctcgac gttcctatgt atttcggcag
     4141 cctgaaagat tgtatgaagg tcagaccatc aaattagacc agttgaaaca gcgattatat
     4201 caagctacac agcaaattta tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa
     4261 ttggaacaag tggctccgct ttatcgagtt aaagcagctc gccaagaaac tgcttatcta
     4321 gaaaaacgct tagaagagaa aatccaacaa tatatgaaac aacagcagca gcgttttgct
     4381 caagctgttc aatctcttga tttgttgagt ccattgaaaa tcatgggaag aggctacagc
     4441 tatacgacgt taaatgatca agtagtaaaa agtgtcaccg agattcatca aggggatttg
     4501 ctgacgatcc attatcaaga tggaatcgtt aaaggacaag tacaagagat agacaaagga
     4561 gaataaaaat atggaaaatc caacttttga agagtcactg caagaattag aaaaaattgt
     4621 catgcagtta gaacaaggag acgttccatt agaatcagct ctagattcgt ttaaacgagg
     4681 aatggaatta agcaaacatt gccaagatac attagctaaa gcagaagaga ccttgacgaa
     4741 aatgatgaca gaatcaaatg aagaagttga ttttgacgga aacgaggaaa gctgagatga
     4801 gattaactga cttttcagct attcatctac ctgctgtaga aaaagaaatc ttgtcttttt
     4861 tagatgagca tacaacggat agaggactct atgatgcgat gagttattca gtgaaagcag
     4921 gcgggaaaag aatacgtccc ttattacttt taacggccgt tgcttccttt gatgagccta
     4981 ttgatgttcc cgtctatcaa gtagcagctg ctttagaaat ggtgcatacg tattccttga
     5041 tccacgatga tttgcctgcg atggacaatg atgatcttcg tagaggaaaa cctaccaatc
     5101 ataaagtatt cggcgaagca ttagctatat tggccggaga tggtttgctt acaggagcat
     5161 tccagctaat cagtatggca cacttaggta attctcctaa gcttctgctg cttcagcaat
     5221 tagctgtttg tgcgggatca caaggtatgg ttgctgggca agctgcagat atcgagggcg
     5281 aatcgaaaaa gctttcttta gaagaattag catttattca tgaaagaaag acagggcatt
     5341 tgattcgtta tgccttgctg gctggtggca tattagcgaa gcaacctgaa gaaatcttat
     5401 tgctacttca acgattagcc gaacatttag gtttagcttt tcagataaga gatgatctac
     5461 tcgatgtgat tggtacaacg aaaacattag ggaaaacagc tggtaaagat gaacgtatgg
     5521 aaaaaaatac ttacccgcgt ttattaggcc tagaaaagac acgtgaggcg ttggagattg
     5581 agctgttatc cgccaacaag atcatagaca aattagaaga ggacgttttt tcttttgatg
     5641 gcgggctgat gcgccagatg atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga
     5701 aaaagaacgc gtagatgtat tagcggtcaa acaaggatta tttgaaacaa gagaacaagc
     5761 caaacgttca gtgatggctg gtttgattta caacgaaaaa aacgagcggt ttgataaacc
     5821 gggagaaaaa attccagtga gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt
     5881 ttctcgaggg ggattaaaat tagaaaaagc actcaaagca tttgatctat ctgttgaagg
     5941 aaaaacattg ttagatattg gtgcatccac tggcggattt actgatgcag ctttgcaaaa
     6001 tggcgcaaaa atgagttatg ctttagacgt tggctacaat cagctagctt ggaaaataag
     6061 acaagatcct agagtcgtcg taatggaaag agtgaatttt cgttatgcaa aacccgaaga
     6121 ctttacctta ggaattccag aagttgctgt tatcgatgtt tcttttattt cactaaaact
     6181 gatgctgcct ccattacatg ctattttaaa agaagaggga gaagtgattg ctttaatcaa
     6241 accccaattc gaagcaggca gagaagctgt agggaaaaac ggaattgttc gtgaccctca
     6301 gacccatcaa aaagttttag aagacatttt gtcatttgcg gcacaaggtg gatatgatgt
     6361 gctggaatta agttattctc cgatcacggg gggagaagga aatatcgagt tcttggctca
     6421 cttgaggaaa gtacccgaaa gcggaacaat caattcagct attaatatgg cagaagtggt
     6481 ttcaaatgca cacgaacagt ttgaccataa ataaattgta actgtgaaaa tcaagtgaga
     6541 agtatgcgga ttcttcttta ttataccaag gaaataagct atcattgatt aagaagaata
     6601 tgctaaggag cgaagacgta atgagaaaaa aagatcggca tcgtttgatt actcgattgt
     6661 tgagtgagca agatatccga aagcaggaag aatttgttga aatcctgaaa agcaaaggaa
     6721 tatctgttac tcaagctacg atttccagag atataaagga actgaaactg atcaaggtac
     6781 cggcttcaga tggcggctac cgttatagca taccagctga aacaaacgaa gatgtaaata
     6841 ctaaattgga aaaactatta aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg
     6901 ttattttgaa aacattgccg ggaaatgctt cggcagcagc aaatttgatt gataaacgtt
     6961 acaagaaaga attattttcg attatcaatg atgacgataa tgttttgatg attacacgaa
     7021 ctgaagacca ggcagtttta ttgaagaaag acttcctgca ttacctttaa gttgaggagc
     7081 gaatgtgatg ctacaagaaa tcagtattac taactttgcg attattccgg aattgcggct
     7141 ttcttttcac gaaggaatga ccgcgctgac cggagagact ggtgcgggta aatccattat
     7201 tatcgatgct ttaggtctgt tagcaggagg aagaggttca agcgattata tacgtcaagg
     7261 agcagaaaaa tgtgtgcttg aagggttgtt tgaactacca aaacaagaag gattctctga
     7321 actgatggtt gaattaggga tcgaaacaga tgaggacaat ctgattgttc gacgggatat
     7381 gtccttaact ggaaaaaatg tttgtcgagt gaatggtcat attatcacat tagctaatct
     7441 gagaagaatc ggcagttatt tagtagatat ccaaggacaa aacgaacatc aggaactgct
     7501 ccagcctgaa tcacatcttg ctttattgga tcgttttgga gatgtagctt tccaacagaa
     7561 gaaaaagagt tatcagcaag aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa
     7621 agtgcagcaa aatgaaaaat cctatgttca gagaatagat atgctccatt tccagcaaga
     7681 agaaatagct gctgctgatt tgcaagtcgg agaagaagag aaattaaaag aagaaagaga
     7741 aaaacttagc aattatcaaa aaattgctga tggattagct gcaggttacg gagcactgac
     7801 gaataatgaa caaaacagtg tagatggtgt tggcttggcc gtttcagaac tgcaagggat
     7861 cgcccatcta gatgtggagt atgaagcaat ttatgaaaat atccaaagtg cttactattt
     7921 gctgcaagat gcaatcggcg atatgagtcg tcagattgat ttattggagt tagatgagag
     7981 tcgtttagaa gaagttacgc aacgtttaga attaatccga cagttgaaac gtaaatacgg
     8041 cgaatccatt gagtcaatat tggcttacta tgacgaaatc accgaagagc tagcttcttc
     8101 tgatttttca gaaggtcaat tggacaaaat gaaagaggaa ctagagcaaa aagagcttct
     8161 acttcagcaa caagccgcag atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga
     8221 aaaatccatt ctgcatgaat tgaaaagtct gtatatggaa aacactgagt ttgaggtacg
     8281 tttcttaaaa gaagaaaata gacaattgaa tcatgatgga tttgaccaaa tcgaatttta
     8341 cattacgacc aatccaggag agccactgaa gcctttggtg aaagtcgctt ctggtggcga
     8401 actgtctcgg atgttgcttg ctttgaaaac tattttttct tctgagcaag gtgtgacaag
     8461 tattattttt gatgaagtgg atacaggtgt gagcggaaga gttgcacagg ccatcgcaga
     8521 taaaatttct caaatatcga aatactctca agttttgtgc atcactcatc tgcctcaagt
     8581 tgctgcagtc gccgactatc aatattatat tgtgaaagca gtgattgatg gacgtacgca
     8641 aacttctgtt tcagagttga aaacaaaaga aagagaagaa gaaattgcac gtatgctggc
     8701 tggaagtgag attacaaaac tcacactcga acatgcaaaa gaactattga aactcgcaaa
     8761 aaggacctga gccacctcgc agtggttcag gtccttttca gcagctgata gctatatttt
     8821 atttttacat gacactcatt acagcagtca atacaacaga tccaatcgtt aaaatcagca
     8881 ttgtccaaat gacaaatttt gttactttag caaaagtact tgtttgtttt ttttcacgca
     8941 ttgttttcaa cctcgtcatt aattagttga ttcaagtgta caatcttttt tccatttcca
     9001 caagagggct tttttataaa aacttccctt tttttaaata ataggtagtt acgatacaaa
     9061 taaataatgt taatagaact agccatataa aggcatcatc cctttcagca ataggtaact
     9121 taatgttcat tccatagata ctgccgacga ttgtcggtat agttaaaata atcgttaaag
     9181 aagttaaaat tttcataata ttattcagat tattcgatac gattgctgaa aaagtctgat
     9241 tcatttgttc tactagtttt aattgaatgc gagaagtagt cattgcctgc tctgtttcca
     9301 ccaatacatc acgtaaatga tttttcaaag catgattttc ttggaaaagt ttattttgcg
     9361 ctagttcttt caatgcatct aaattagcag aagtcgcttc tctgaaataa acgaggcttt
     9421 tttggatatc catgatttga taaagttgtt tgttttcagt ggatacttga agtttggctt
     9481 ccaaagtatc gcattgagat tgaagcgtag ctaaataact gttgtaagag agtgtgacct
     9541 gccaaatgaa ttgaaagaag atattgagat taggcgaaat atcacttttt ggaaaggtct
     9601 ggctaaagac tttctttaaa aacagtggtt catgattcgt aattgtgata agcttctttt
     9661 tgggcgtcaa aataatagag agcgggtatg tatccacttg aaaataaccg ctaggacttg
     9721 ttttagcaaa aggatattgt aacaataata acgctggctt tgtcaacttt gattgattga
     9781 attcttctgt acgggaattt tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa
     9841 tttcaaattg atgtgtcagc tgatgaatct cttcttgatt aggacgttct acacaatacc
     9901 agatcgtttg gtcagtttcc tttccggtat tacgcagttt ctcattatct aatgtaaaat
     9961 atttgatcat gattttcctc ctgttgcctg tttcttttat tataacggat agggaattgg
    10021 aatacatgga atgtgtttgt aagatataga atataactgc ataaatttaa ataaaatgaa
    10081 acagttgtgt aaagtatata cagtatactt gaagcaacga taaaaaagga gtttacgttc
    10141 atgggcacat tcacaccact tatcttgttt tggtatattt acccggtaat cgttttgttt
    10201 ggttgccaat ttcttgtaaa attattttcg ttaaatcgtc gttttaaaat aaaagcccct
    10261 gatttggcga ttcctttttt gtggggcggt atgcatgcat tgtctagaaa tacagggaca
    10321 atttctatct taccgttttt ctttatttct gttttgctaa tgggaatttg tattgctgtt
    10381 ttccaagcat attattatga agaaattatt tatccgcgtt atttcaagat gacttggcgt
    10441 ttggtttttc tgttgacaat cgtcttgtac gctgtcttga tcattgtgaa catactttct
    10501 tacctatgaa aaaaatagaa aagctgtaaa aagtctttta aaaggctttt tgcagcttga
    10561 tttttaaaaa aatgtttatg aataaaaaaa gttgttcccg gatttcccag aaaaggtggt
    10621 agaaagtggg ggattgtggt agactaattt tagttagtgg aggaatgggg ggctttcaat
    10681 gttgatgggt gaattccaac ataatattga tgcaaaagga cgtttaatcg tgccctcaaa
    10741 acttcgtgaa gaacttggcg aaaagtttgt tctgacacgc ggattggatg gctgtctttt
    10801 cggctatccg atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc
    10861 taaaaaagac gcacgtactt ttgtgcgatt cttctattcg gctgccactg aatgcgaact
    10921 tgataaacaa ggacgaatca atatacccag cacgttaaga aattatgcag ctttgacaaa
    10981 aggttgcgta gtcatcggtg tatctaaccg aattgaaatt tgggatgaga cacgatggca
    11041 agatttttct gcagcagcag aagagaactt tgacgaaatt gctgaaagta tgattgattt
    11101 cggactataa gaataggaag aggctgacta atgactgaaa catttcaaca ctatacagtc
    11161 atgttgaaag aaacggtaga tggattgaac atcaaaccgg atggcgttta tgtagactgt
    11221 acactcggcg gtgccggaca tagtgaatat ttattaagcc aactaagttc taaagggcat
    11281 ctgtatgcgt ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg tttagcttct
    11341 tatgtcgaac aaggtatggt cacatttatc aaagcgaact tcagagaatt agaagaacgc
    11401 ttgagtgagc atgtagataa ggtagacggc attttgtatg atctaggggt ttcctctccc
    11461 cagctagatg aagcagaacg tggatttagt tatcatcaag atgcaccgtt ggatatgcgc
    11521 atggatcagt cagctccttt atccgcttat cacgtaatta atgaatattc ctaccatgaa
    11581 ttagtaaaga ttttcttccg ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa
    11641 atcgaacgag tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt
    11701 aaacaagtca tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt
    11761 caagctgtgc ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc tttagaacag
    11821 gcaatccggt tattgaaagt caatggaaga atcagcgtaa tcacattcca ttcattggaa
    11881 gacagaattg tcaaaagtat gtttaaagaa tatagtacgg tacaagatct accaccggga
    11941 cttcctgtca tacctgacga attccaacca gaactgaaag tcattactcg taaaccaatc
    12001 cttccttcag aggaagagct aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt
    12061 gcagaaaaaa taagaatgaa agaggagtag aatgatggca gaattaaaga aaatgcaaga
    12121 ttacgaatat gatttacctg aattggtgga tcagccggaa caacaaatgc ctgattcttc
    12181 ttcgaatcaa gaaatcattc ttccacaatc accggcaaaa cgattaaagc agatttctgc
    12241 tttggaaaaa attgctgtgg caagcgtggc gattgctatt gttgctttat gtgttttaac
    12301 agttatgctg cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga
    12361 aatcaatgat aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg
    12421 tactgaacgg attaaacaaa tcgcagaaga aaaaggattg tctattaatg acgacaattt
    12481 aaggaaagtg aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa
    12541 tctaaatccg atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg
    12601 attgttcttt ttatttgccg tccgtttctc gtatatcgtt attggaggtc atgttgctgg
    12661 tacctcatta gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag taaaagcaaa
    12721 aagaggaacg atttatgatc gtaacggtgt ggcgctcgca gaagatgcct cctcttattc
    12781 aattaaagcg atactctcaa aaacatatac ctctggggat aaaaaactgt atgttgaaga
    12841 aaagaatttt gataaaatag cagaaatcct gcataagaat ctttctatag acaaaaaaga
    12901 tgcactgaat attttagagg acggcgcaaa aaaagagctg tatcaagttg aatttggtag
    12961 ctatgggaaa aatatctccc aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga
    13021 aggtgtcgct ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt
    13081 ttcttctcat tttattggat acgcagttcc agacaaagat gagaatggct tagttggtaa
    13141 actaggtttg gagtctgctt acaatgatat cttgagcggt aaagatggta agattattta
    13201 tcaaaaagat aattttcaaa atcctctgcc aggtacagtg gcagaagaag aaaaagctgt
    13261 cgacggacaa gatatctaca cgactttaga cagcagacta caaagttatt tagaaacact
    13321 gatggatcaa gtaaatgaag aatatcagcc agaagaattg acagcagtcc tgatgaaagc
    13381 aaaaaccgga gaaatcttgg caatgggaca acgacctact ttcaatcctg aaacaatgga
    13441 aggattgact ggaaaagatg ctatctggcg taatttcttg gtacaagata gttatgaacc
    13501 aggatcaacc atgaaagttt tcactactgc agctgctatc gaagaaggag aattcaacga
    13561 gaatgaaacc ttccaatctg gaaaaatcca agtagaagat gctacgatca atgaccatga
    13621 ctttggtgaa aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg
    13681 gatggtcata ctggaacagc gattaggcgg acgatggtat aactatttac aaaaactggg
    13741 atttggacaa agtactcatt ctggattaga tgatgaagta aacggtgctt tacctacatt
    13801 aaatattgtc gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga cgaatttcca
    13861 aatgatgaaa gctttcactt ctattgcgaa taacggtaca atgattcaac ctcgatatat
    13921 cagcaaagtg gtggatcccc aaactggaga agagcgaaca actcaaacag aagttctagg
    13981 gcaacctttt tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag atgttgtcga
    14041 aagcgaaaac tacggtattg cttatggtgt atatagtgta cctggataca atgtatcggc
    14101 aaaaaccgga acagcccaaa tcgcttctga tacaggggga tatcaaacag gagatacagc
    14161 ctacctgtat tcgattgtgg agatggttcc ttctgaagat ccagactatg tcttgtatct
    14221 aacaatgaaa caccctaaga catatgatcg aatggcattg gctaaaatcg caaatccatt
    14281 gatgaaacgg gcaatggatt ttaaagaaac tgaagaagat tcagatacag aaacaaaaac
    14341 agaaaaagta tccgtagccg attatcgtaa tcttgaagcg gacgttgcag cagctgatgc
    14401 tcaaaaaagt ggactgcaac ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc
    14461 tactgccaac ggtgatcagt tgatctctgg agaaaaactg atcctatata ctggtggtga
    14521 taagctgatg cctgacgtaa caggatggtc aaaagcagat atcatgaagc tggggaaaat
    14581 cctcggtatt gaagttagtt ttgatggaga tggttattgt gtcaaacaag aacttgctcc
    14641 atatgaaaaa atcactgaag acaaacttag ttttacttta gaagaataag aagagaaata
    14701 ggagagaaaa gagcatggaa tggacacaag cattgattcc cattgtcagc agttgtgcaa
    14761 tgacaattgc cgcaatgccg ctatttattg gttattttca aatgaaaaag caaggtcagg
    14821 cgattcgtga ggagggacca aagtggcaca atagcaaagc cggaactcct acaatgggcg
    14881 gcttagtgtt tctgatcggc tctattttga caggaatttg ggttggagct tggcaaaaac
    14941 agctaactcc tactcttttc atcttgcttt ttgtattagc gttatacgga gtaatcggct
    15001 ttcttgatga ttttataaaa atattcaaga aaagaaacat gggactgaac tcgaagcaga
    15061 aattgattgg acagattcta gggggaatca tcttttatct tgtttaccgt tctgaaggtt
    15121 atccaggtgt attgaatttc ttcggactag atctaccttt aggaatcatt tatggtattt
    15181 tcgctctctt ctggcttgtt ggattttcaa atgcagtaaa tctgacagat ggcatcgatg
    15241 gattagttgc tggcttagga agtatctctt ttgcagctta cggtctaatt gcttggcatc
    15301 agcatcagta tgatgtatta atcatttgct taagtgtttt aggaggttta ctgggtttct
    15361 tcgtctacaa cagaaaacct gccaagattt ttatgggaga tgtcggttca ttagcgttgg
    15421 gcggactttt agcggcaatt tctataatgc taaaccaaga atggacattg cttttgattg
    15481 gtttgatcta tgttatggaa acagcaagtg tgatgcttca agttacttcc tttaagctta
    15541 caggaaaacg aatttttaaa atgtctccaa ttcatcatca ctttgaaatg tgcggatggt
    15601 cggagtggaa gattgataca gttttttggt tgatcagcat tgtgacttcg ttgatcacat
    15661 tatggatcgt ttggtaggag atgacgggaa atgaaaaaaa tatcaacata tgaaaataag
    15721 aaagtgcttg tcttaggact cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa
    15781 ttgggcgcac ttgtcaccgt taatgatgga aaaccttttg acgaaaatcc ggaagcacaa
    15841 gagctgcttt ctttagggat aaaagtaata acaggtagtc atccaatcga attgttggat
    15901 gaagaatttt cattgatggt caaaaatcca gggattccct acagtcatcc tttagtagca
    15961 aaagcccaag aaatgggtat tccggtaatt acggaagttg agctagctta cgaagtagcg
    16021 gaatgcccaa ttattgggat tacaggaacg aacggaaaaa caacgacaac aacgatgaca
    16081 ggactactac tgaatgcagg agcagatcaa ggaattgcac gtttagcggg taatattggg
    16141 tatccagcaa gtggtgtagc gcaagaagca aaatcagaag ataaaattgt gatggaattg
    16201 tctagcttcc aactcatggg aatcacagat ttccgccctc atatagcggt gattaccaat
    16261 atctatgaag cacatattga ttatcatggt acaagaaaag aatatgtcaa agctaaatgg
    16321 aaccttcaaa aaaacatgac agaaaaagat tatctgatcc ttaactggaa tcagtcagaa
    16381 ctacaagaat tagcgcaaag aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg
    16441 ttggaagacg gtgtgtatgc tgacgattac tcaatatact ataaaaaaga aaaaataatg
    16501 gaaatatctg aacttggcgt accaggtaag cataatgtag aaaatgcact agctgctatt
    16561 tctgtagcaa aattgtatgg tatatcaaat gaagctatac gagaaacgtt gcattttttc
    16621 catggcgtac cacatcgtac gcaatatgta ggagaaatcc aaggcagaaa attttataat
    16681 gattcaaaag caaccaatat tttagcgact aaaatggctc taagtggatt tgaaacgtct
    16741 aaggtcgtgt tattggctgg cggattagac agaggaaata catttgatga attaattcct
    16801 tcattaaaag gaatcaaagc aatggtcgta tttggccaaa caaaagaaaa gttgatggat
    16861 gctggaaaaa aagcaggcat cgagacaata gtaacagccg attctgtaga acaagcagtt
    16921 cctttagcat tagaaaacag tacagatggt gatgtagttt tattatcacc agcgaatgcc
    16981 agctgggatc aatacccaaa ttttgaaact agaggaaaca gattcatgga agctgtgaac
    17041 cgattaaaat agaaaaagta gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg
    17101 aggaacaggg ggacatattt atccagctct tgcctttgtc aattatgtaa agactcaaga
    17161 accgaatgct gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc
    17221 tgatacagga atgcctttcc ataccttgga gattcaagga tttaaacgaa aaatttccat
    17281 gcataacatc aagacggtcc gattgtttct taagagtatc cgtcaagcaa aaaaaatcct
    17341 cagtgatttt caaccagata tcgttatcgg tactggagga tatgtttccg gagcggtagt
    17401 gtacgctgca gccaaaatgg gtattccaac agttatccat gaacaaaaca gtgtaccagg
    17461 tgttaccaat aaatttttaa gccgatatgt ggatcggatc gctctttcat ttaaagatgc
    17521 ggcttccttt tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag cacaagaagt
    17581 agcagatact aaaaaatcag atattttgaa aacatttgat ttggatccag aaaagaaaac
    17641 agtcttgatt tttggcggta gtcaaggagc acttaaaatc aatcaagcag tatcagtttt
    17701 tttaagtgaa tatattcctt cttcttacca agttttgtat gcttcaggag atagatatta
    17761 tgaagagatc ctaaataaaa taaatcctga aatacaaaat attagtgtcc agccttatat
    17821 caaaaatatg gcagaggtca tggcttccag tgatctgctg gtggggcgtg ctggtgcaac
    17881 ttcgatcgca gaattcacag ctctggggtt accggcagtg ttggttccca gcccatatgt
    17941 tacgaatgat catcagacta aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat
    18001 gattgcagat aatgaactaa caggtgaatc actaagtcag acagtcaacg aaatcatggg
    18061 tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc
    18121 ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa aaataaaggc ggtggtctta
    18181 gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt cttctgatga aaacaatttg
    18241 actccttggc aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa aaaccaagag
    18301 ccaaaaaaag aagacataac attagataca cccctttcag attctgaaga agaaaaaacg
    18361 gttcaagagg aacagcaaga agaaagccag tccgagaaaa aaatcgaatt ttttgaagag
    18421 atcaagcaaa agaaaaacgg tggaccatat aacggttctt ttttgaaccg gctgcccaat
    18481 ttaaaaagtc aacggcacaa agtactattt cgtcggttag ctttgatcat cactgtattg
    18541 ggtataccgc tgattttttt gatctattat gtttctcctt atagcaaatt acaagcagtt
    18601 gcagtatcag gcaataagac agtcaacagc caagaaatca tctctgatac aaaattgtca
    18661 ttaggagaaa atgtttgggg acagtatttt catcgatcta cttatatcga tcgccttaaa
    18721 aaagcacagc caagaatcga aacagcaaat atacatttca aagggatgaa tgaattcgat
    18781 ttggatgtaa cagaatataa agagattgct ttgatcgcaa aaaacaatca atactaccca
    18841 gtgattgaaa atggaacagt attagatgaa aaagtagcaa atcctacaaa aaatctccct
    18901 atcttggaag agttcaaaga ctctgcaaaa atcaaagaat taacaaagca atacaatcag
    18961 ttatcatcag aattacaaaa agcaatttct gaaatcaagt acacgccaag agcaagcaat
    19021 aaaaatctga tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa tatcaataat
    19081 cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag atatggatga aaaaggtgtg
    19141 atcgacatgg aagttggtat cttctcgcat ccttacggta gcactcaaga aacggaagaa
    19201 tcgggccaat cggaagaaac aactgaaagt gaagaaaatg cggatcaaac aaacgaatca
    19261 agtgaagagg cagaaacgca aaaagcacaa acacctggat cgtctagcac aaacggatca
    19321 tctgattcaa atgattcaag tgaaacaagt caaatagaag ataatccaag tacaactccg
    19381 tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa
    19441 ccagtaattt atggtagaat tggaagcagt gaatatatct attaataagt atattttaaa
    19501 acggacgcta gttgttcgct tgtatatgga atagtaagta ggagggaatc ccattcatgg
    19561 caaaaacagg aatgtatgta ggccttgata ttggaacgac atctgttaaa gttgtcgtgg
    19621 ctgaatatat cgacagccaa atgaatatta ttggagtagg aaacgcaaaa tcagaaggga
    19681 ttaaccgagg cattatcgtt gatatcgata aaacggtcca agcgatacaa cgagcagtac
    19741 gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa
    19801 acatgcttga agtagaaaat tgccagggaa tgattgcagt taacggagat tcaaaggaaa
    19861 tcaccgatga agatgtcaga aatgtggctt cagctgccct tgttcgttca atacctcctg
    19921 aacgtcaaat cgtgtcaata ttgccacaag attttactgt cgatgggttc gagggaatca
    19981 aagatcctcg tggaatgatc ggcgtaagat tagaaatgta cggtctgttg tttacaggac
    20041 ctaaaacgat catccataac atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg
    20101 aaatggtgat tactccttta gcgctgactg aatcaattct ttctgacgga gaaaaagatt
    20161 ttggtacgat cgtcattgat atgggtggcg gccaaacaac aacggctgtt atgcatgata
    20221 agcaattgaa gtttaccaat cttgaccaag aaggtggcga atttgtcact aaagatatct
    20281 ctattgtatt aaatacgtca tttaacaatg cagaagcgtt gaaaataaac tacggggatg
    20341 catacccaga acggacatca ccagatgaag aatttccagt agatgtgatt ggtcagtcag
    20401 aaccagtgaa agtcgatgaa cgctacttgt cagaaattat ttcagctcga atggaacaga
    20461 ttttcaacaa agcaaaagat gcattggacc aaatcgaagc attagagttg ccaggtggcg
    20521 tcgtattgac aggcggagct gctagtcttc ctggtgttgt ggacttggca caagaaatct
    20581 tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg gctgagaaac ccggtattta
    20641 caaatgtaat aagtatcgtg gattattcag caaacttgag tgaagtgtat caattggcaa
    20701 aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa
    20761 caaacacata tgagagctat gaagcacctg aagaaacaat ctacgatgag ccggaaccaa
    20821 aagagtccgg agaaaatgta aaaaataaaa tcaagggttt ctttacaaat atttttgatt
    20881 aattcatcct gggaggaata agatatggaa ttctcaattg ataataatat caatgacggc
    20941 gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt
    21001 gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca cagacgtgca agcattaaaa
    21061 aattcaaaag ctgaaacagt tatccaatta ggccccaaat atactcgtgg tttaggtgct
    21121 ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa
    21181 gctttagacg gtgcagatat gattttcatc actgcaggaa tgggtggagg aaccggtaca
    21241 ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc
    21301 gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc
    21361 gctcgtttga aagaaaatgt ggatacatta ttaattatct caaataaccg tttattagaa
    21421 gttgtagata aaaagacacc gatgcttgaa gctttccgtg aagcagataa tgttttacga
    21481 caaggtgtac aaggtatctc tgatttgatc acagcgccag gttacgttaa ccttgacttt
    21541 gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat tgatggggat cggtgtggct
    21601 agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa
    21661 acatctattg atggagctga acaagtactt cttaacatca caggcggttt ggatatgaca
    21721 ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat
    21781 atcattctag gtacatctat caatgaagaa atgggcgatg aaatccgtgt gacggtcatc
    21841 gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca
    21901 caaatgcaat cacctgctca aaagactgta ttagatatgg atcaagctaa accaacttct
    21961 tcagaagaag aaaacagttt tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct
    22021 agagttgatg attccaattt tgacaatatc gaaaaaaaag aatttgatac attcaaccgc
    22081 gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa
    22141 aaataaagga gaggaagagc acatgattgc tgacaacttg cagaaaatca agcaagaaat
    22201 tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa gttacattag tagctgtaac
    22261 aaaatctgtt gaatccgctc aagctgaaga attggttaga gctggcgtta tgaattgtgc
    22321 agaaaaccga gtagataaat tattagaaaa gaaacaagat ttatttgatt ttcctgaaat
    22381 aaagtggcat ttaattggta atttacagcg cagaaaggta aaattgattg taaatgaaat
    22441 tgattacttt catgcgttgg acagtttaag attagcagaa gaaatccaga agcggttaga
    22501 gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat
    22561 ttcaccagat gagcttttac cgtttgtgga atctttagct gcctgcacaa aaatcaaggt
    22621 tgttgggttg atgactatgg cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt
    22681 tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg
    22741 tagtgaatta agtatgggga tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac
    22801 atttatccgt gtaggaacag cgctattcaa agacgaataa aggaggaaca aaaatgccgc
    22861 ttttaaacag aggagcaatt gctaattttt tcggattagc tgatgaggaa gagtacgaat
    22921 accaagatta tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac
    22981 agaatcaatc aactcattct gtatctgctg cgaatactca tgaaaaacaa aaaacggcga
    23041 cttcacgtcc tgtaacgcat gaaagtattc agccggctga aaagaaaaaa gtaattgaac
    23101 tacatcaatc acctacaaaa actgtagcga aagaaagaac agcttcaaga acctctacac
    23161 aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga accaagagct tattctgaag
    23221 ctatggcgat tgctaaacga atcattgctg gagaagcaac cttagtgaat tttcatttag
    23281 tagaagaaaa acaagcgaga agaatcgttg attttctaac aggaacagtt tatgcactag
    23341 atggagatat caaacgtgtt ggagatgaga tttttctttg tacaccgact gggatggaga
    23401 ttgacagttc aaccgctcaa tcttttgcag atgcgaattt gtttgatctg taagaggagg
    23461 aactacgatt tatactattg cactattact tcatttactt caacgggcag cctatttcta
    23521 tagcattatc ttgatggtat atgcattgct ctcttggttt ccaggcgggt atcaatcagg
    23581 gctaggacga ttattagcta agatctgcga accgtatatc agtttgttcg atcgtctgcc
    23641 tttgaggatt ggacccttgg atttcacaat tttaggggca atcctgatct tgaacttagc
    23701 cgtacaaggt ttagcacaga tcgttatatg gataactgtc catttagttt attgattgaa
    23761 gcaacaaaat ctttattctc agaatagata ggaggggaaa attttggatg caaatgtata
    23821 ccagcacttt cgggcagatg aacgtacatt tatcgattcg gtaggcgatt ggattgaaca
    23881 ggttcaagga caatatgcac cctatctgac cgaatttctt gacccaagac aatcctatat
    23941 tttagaaaca ctgattcgac aaagcagtga tttgcgtttc atgttttatg gcggatatga
    24001 acaggcagaa agaaaaagat gtttgatttt ccccgattat tacgaacctg tagaggaaga
    24061 ttttgaaaca gcattgtatg aaatccatta tcctagcaaa tttgccagcc taagtcatgg
    24121 gaaaatcctt ggaactttag tgggaacggg gatcaaacgc tcttactttg gcgatatcat
    24181 ctcggacggt gatcgctggc aagtatttat tgcaaatgag gtggatcatt ttgttgctgc
    24241 acaagtaaca aagattggaa aggttgctgt acgcttggaa ccaatcaaat acacagaaat
    24301 gattatgccg aaagatagct ggacacaaga aagaggaaca gttagttcca tgcgactaga
    24361 cagtgtgatc tctgagattt ataatatttc cagacagcgg tccaagcagc tgatcgaagc
    24421 tggtaaagta aaagtgaact ggacagaaaa tacaagacct gattttgtac ttgaactatt
    24481 agatattatt tcagtcagag gttatggcag agtccagatc caagaacttg aggggaagac
    24541 gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt aaataaaata aaaaggtggt
    24601 ttactcatgg ctttaactcc attagatatc caaaataaga cattcccgac aaaaatgcgg
    24661 ggatataatc aagacgaagt agatgatttc ttagatttag ttgttcgtga ctatgaggaa
    24721 ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac attctgaaga aaaacttgag
    24781 tacttcaatg aattaaaaga tgctttgaac caatcaatca tcgtagctca agatacggct
    24841 gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac
    24901 aaagcagatg agttagttgc gaacgctgaa aaacgtgcac atcaattaac aactgatgca
    24961 gaagaaaaag caagaaaaat cttgacagat gcaacagaaa aagcaagaca gcttgcgaca
    25021 gaaactgaag atcttaagaa aaaaacacga gtattccatc aacgtatcag cttgatgctt
    25081 gagtctcaat tagaacaagt gaaaagtccg gaatgggatg aaattttaca accattctcc
    25141 agctacgtga ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat
    25201 gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag aagcacctat gacggcattt
    25261 gatacacagg ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga
    25321 aaagcacttt gttattctta aagcgagccg atgagagtgg aagttcggta agacactgca
    25381 tggtgaaaga tcctctactt gagacctgct tttccgaaat tagagtaaga agcagcggct
    25441 gacctcgtta ttggtcacag agagtttgca ctagatagat gcaactaaat ttgggtggta
    25501 ccacggcatg ttcgtccctt gcggctgaac atgctttttt gtgtccggaa aacaaataaa
    25561 ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat taggaaaaac aaaattccct
    25621 atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa aagaatggga agaaaatcga
    25681 atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta gctttgttct tcatgatgga
    25741 cctccatatg ccaatggtaa tatccattta ggacattcat tgaataaaat cagtaaagac
    25801 attatcattc gttcaaaatc aatgtctggc tatcgtgcac catatgttcc aggatgggat
    25861 acacatggat tgccgattga acaagtattg gctaataaag gcgtgaaacg taaagaaatg
    25921 tccatggcag aatacttgga aaaatgtcgt gaatatgcac tttcccaagt agataagcaa
    25981 cgagcagatt tcaaacgcct aggagttgca ggagattggg aacatcctta tgttacatta
    26041 gatccttctt atgaagctgc acaagtacgc gtatttggta aaatggctga aaaaagctat
    26101 atttacaaag gattgaaacc aatttactgg tctccgtcaa gtgagtcttc tttagcggaa
    26161 gctgaaattg aatataaaga tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa
    26221 gacggcaaag atattcttga tgaagataca gcttttgtca tttggacgac aacaccatgg
    26281 acattgccag caaaccaagg tattgctgta aatccaaaat ttacgtatgt attggtggaa
    26341 gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca atcagaaatc
    26401 ggttgggaaa atgtaacagt tctaaaagaa tttgctggac aagaaatgga atatatgact
    26461 gcaagtcatc cattctatga acgtgaatca cttgtgattt taggcgatca tgttacttta
    26521 gacgctggta ctggattagt ccatactgct ccaggacatg gggaagatga ctatatcgct
    26581 ggtagcaaat ataaactatc tgtagtgtca cctgtagact caaaaggatt gtttacagaa
    26641 gaagcacctg gatttgaagg tatcttttat gacaaagcaa atcctatgat cactgatcta
    26701 ttaaaagaaa aaggagcttt gctgaagcta gatttcttta ctcatagtta tcctcacgat
    26761 tggcggacga aaaagcctgt gatctatcgg gcaactccac aatggtttgc atctatcgat
    26821 aaattccgtc agaatatttt ggatgaagta gaaaaagtag agtgggttat cccatggggt
    26881 aaaacacgtt tgtataatat gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt
    26941 gcatggggag taccacttcc gattttctat gctgaaaatg gagaagcaat catcacacca
    27001 gaaacaatcg aacatgtggc aaaattattt gctgaacatg gatcaattat ctggtttgaa
    27061 aaagaagcga aagaacttct tcctgaagga tttacccacc caggttctcc taatggcgaa
    27121 tttacaaaag aaaaagatat catggatgtc tggtttgatt ctggttcttc tcatgaagct
    27181 gttttacgtc agcgaccaga attatcattc ccagcagata tgtatttaga aggatcagac
    27241 caatatcgtg gctggttcaa ctcaagtatc acgactagtg tggctatcaa tggtgtagca
    27301 ccatacaaat cagttatttc tcaaggattt gctttagatg gtgaaggccg taagatgagt
    27361 aaatctttag gaaacatcat tgcaccagaa aaagtgatca aacaatttgg tgcagatatc
    27421 cttcgcttat gggtatctag tgtagattat gaagcagatg ttcgtgtttc tatggatatc
    27481 ttaagtcaag tggcagaagt atatcgtaaa atcagaaata cgatgcgttt cttattagcg
    27541 aatacagaag atttcgatcc taaaaaagat actgtttcgt acaatgattt acgttctgta
    27601 gacaaatata tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga agaagggtac
    27661 gacaaatata acttcatgca tatttaccgt acagttatga acttcttgac ggttgatcta
    27721 tcgtcattct acctggattt tgctaaagat gtagtttata ttgaagcaga agatgattat
    27781 caacgtcgtt gcatgcaaac tgttttctat caaacagctg tagctttgac aaaattattg
    27841 actcctatca ttccgcatac agcagaagaa atctggtcat tcctgaaaga agaagaggag
    27901 tacgttcagt taagcgagtt tcctggttat gaagagtttg caaaccaaga agaattgatg
    27961 gatacctgga ctgctttcat ggacttccgt gataatgtct taaaagcatt ggaagaagca
    28021 agaaacagca aattgattgg taaatctttg gaagcaaaac taactgttta tccaaaacaa
    28081 caagttcgtg aaatgttaaa agctttggat gcagatattg cacaattatt gatcgtttca
    28141 ccagactact ttgaagttat ggcagcagat gaacaagtgc cagataacgc tatggtattt
    28201 gatgatgttg ctattacagt ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa
    28261 gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa
    28321 atcgttgaag acttctatcc tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga
    28381 aaagggggtg tgacatgttc tttgtcacac ccccttttgt tatcaatcgt ttaattttcc
    28441 acgttcacga taccagttat ctggttgcca gatccattcg aatccgtctt tttcaagtag
    28501 ttcgaaagct gctttaggtc ccattgatcc agctttgtag ttgggaaaat ccggttgtgt
    28561 ttggtcccaa gcttttcgaa tgacatccac gatatgccaa gattgagcga cttcttccca
    28621 atgagagaag ttcgttccat caccattcaa cgcatctaat agtaattttt cgtaagcttc
    28681 tgggctattt tcaaccattt ctgcactatt tctaaattcc aatttgattg gttccgtttc
    28741 aaatccttgc cctacttctt tcccattcaa ggataaagaa aatccttcag ttggctgaat
    28801 atagatcgtt agaacattag gtggaagtgt cgtatcgtca catggttcat cgacagatgt
    28861 tttgaataca ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc ctttttcagt
    28921 caaacgtttg cctgtacgta catagaaagg aacacctgac caacggaagt tatcgatagt
    28981 gaatttgcct gcgacgaatg tttcagtctc tgattgagca tccactttgt cttcttcgcg
    29041 atagcccttg aattgtacac catccaattc accttgagca tattgcccgc gaacgaagtt
    29101 ttctaacact tcttcttcgt tataaatgcg gatagctttc aatgctttga tcttttctgt
    29161 acggatttct ttttcggaaa atgctactgg cggttccatt gcaagtaatg ctacaacctg
    29221 taaaatatga ttttgcacca tgtctttaag tgcgccacta tgatcgtagt agcccccgcg
    29281 atcttctaca ccgaggtttt cgccaaaagt aatttggata ttgtcgatat agcgattgtt
    29341 ccattgagcc tcaaaaatat tattggcaaa acggatagct gagatgtttt ggatcatttc
    29401 ttttcctaaa taatgatcaa tcctgaaaat atcttgttct gggaatactt ctctgatttg
    29461 ctggttcaat tcaaaagcag actggtaatc agaaccgaac ggtttttcta tgatcaaacg
    29521 attgaagcca ttttttgtca taatattttg tgatttcaaa tgagaaacga tcgttccaaa
    29581 aaattgaggt gccatagcca aataatagac acggttaccg tttaattgat agtgtccatc
    29641 taatcggtca gacaattctt ttaatgtctg ataatgcgtt gaatcattta catcatggga
    29701 ttgatagtaa aaatggcttg caaaggtagt tgcttcttca tctgaaggat tcaatgcacg
    29761 gattgtttct ttgacaactt cacggtagtg ctcatcgctc cagggacggc gagcggttcc
    29821 gattacagca aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca aagacgggta
    29881 caatttacgc tgggctaggt cacctgtacc gccgaatatc gtaaacaata catttttttc
    29941 attagacatg tggatacact cctttatatt ttatcgacaa aaatcgtgct ggccgcctta
    30001 aagctgacgg ggattttctt tctatcattt tggatagtga tcggtccttc ataagctgcg
    30061 atgtcaacga tctcatattc ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac
    30121 aattcttttt catcaagcac tcgtgcgata cgcacttttg taccgacagg ataggactcc
    30181 aatgtttgtc ggcgttcttc atggacaact tcctcttttt taggaatcga tcctccatga
    30241 ggacaatgtt caggatgatt caaatagttt tctaaatgat ctgcaagatg ttccgaagtc
    30301 acatgttcca atacttcagc atcatcatgg acttcattcc agctatagtc taaatgttca
    30361 actaaaaaaa cttcccataa gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg
    30421 cttgcagtca attgtacacc ttgataaggg gaatgttcta caagctcttc tttcacaagt
    30481 ttagaaatca tttcactgac agatgcagct gagacatcta gactagatac gatttgttta
    30541 ttgttgactt tcgttgtatc tccacctagt tctaaaatga ttttcaaata atcttctcga
    30601 tttggtgtca tattcgatca tcctttcatg gtaaaaaacc atcattagta agtgtaacaa
    30661 aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa ggatttttag acgtttttac
    30721 aacaaaaaac gaaagatgat agagaagact cattctttcg tttataaagg atatatatat
    30781 acattgggag ggatctattt ttcagataac aacttatatg aagtgtctgc agattcgtca
    30841 taaagagttt gttcgggata agaaaagata agataaaaac catactgatc acgtacgact
    30901 aaacgatatt catctatttc taccacagat gtgccaagag actgaccgtt gatcaaaaac
    30961 gtgtaatcag atgtaatctg taattgttgt ttttgctggc tatcactaaa gaaccatgta
    31021 ccttgatact ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt caagtaaata
    31081 aaacttgaga gaacggtaat gagtgaacca agaaaataga tcattttttt tatcaccaaa
    31141 tcactccttt atgagaaaat aatcgcatag acatattctt tcttctttat ttcattgtac
    31201 cgaataaaag ctaaaatttc tctaagactt tcgactggtt tttttactgt ttttctacaa
    31261 attatgatag gatacatagt aggaagaaag gtgacataat ttgaagtatc agcaagtgga
    31321 aatagctttt tttattgacc gcccgaatcg gtttatagca cattgtatga aaaaagacgg
    31381 agaaataatt attgcgcatg tcaaaaatac aggtagagga aaagaggtcc tgataccagg
    31441 cgctgaagtg gctattgtat ttgctcctgg acctaaacgg aaaaccgcct atgatctgat
    31501 tgctgtaaaa aagaagggtg actggtttaa tatcgatagt caattgccta atcggctggc
    31561 aattgatggt attttagacg gcacgatcct tttaccagga ttaactggtc catttacggt
    31621 atacaagcgt gaagtaactt atcttcactc caaatttgat atttatttag agacggtggt
    31681 cggacaaaaa gcatttgttg aagtcaaagg tatgacactt gaaaataaag cgattggtgc
    31741 ttttcctgat gcgcccacac tcagaggatt gaaacatata ggagaattaa cgtatgccgc
    31801 acaagatggt tatgccgctt atgtcttgtt tattgctcag ttcgaacatc ttcatctggc
    31861 aacgatccat gaagaaatgc aaccagcact agctgatatg gttcgacatg cgcagcaaag
    31921 cggagttcaa atcctcgcct ataattgtca agtatcggca gatgaggtaa ctgtcaagca
    31981 ggccattcca tttgatgtag atatcccttt tgaagatcct atacaataga aagaaggtaa
    32041 aataatgatc aacttaggta ttatcggaac aaattggatt actcatcaat ttgtccaagc
    32101 agctttaagc acaaaaaaat atgaattgac agcggtatat tctagacatt tggaaaaagc
    32161 acaggaattt ggttctcatt atgaagggga tatcgaattc gcaacagatt tgaagacttt
    32221 tttcaatatt actcatatgg acaccattta tattgcttct ccgaatagtc ttcactttag
    32281 tcaagctaag caagcaattt tggcaggaaa aaatgtgata gttgaaaagc cagctttttc
    32341 gacgccaaaa gaaatggatg aaatcatcca tttggcaaat gaacacaatg tgtatttttt
    32401 tgaagcagca agaaatatcc atgaatcagg ttttaaaaag attgccgatt acttaccatt
    32461 aaaagacgag ataatcggtg caaatttcag ctatatgaaa tattcttcgc gctatgacca
    32521 agtattagat ggagaagaac cgaatatctt ctcgactcat ttctcaggtg gtgcattgat
    32581 ggatttaggt gtttaccttg tatatgcagc tgttgcttgg tttggaatgc caaaagaagt
    32641 ccattatttc ccaagaaaga tttctactgg tgtagatggt cttggatggg ggatcttacg
    32701 ttatgagaat tttgatgtat cgatccagcc aggtaaaatc ggtgattctt atttaccgtc
    32761 agagatttat tttgactcag gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc
    32821 acaatttcat gatcgcgcgc ataaagaaat cattgatttg gcggtaacag ctgcagaaaa
    32881 tccaatggca gatgaagcag aggattttgc caatgtcatg gctcatccga aagatccagc
    32941 ttggggattg ttgtatgaag agtggatcga gctagctaga aatgtgaacc aagtgattta
    33001 tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag atttttaatg
    33061 aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa aatatgagga taaagggttt
    33121 atctcacctt ctcttattca acgagcagtt tttcagccac taagtaataa acaaagtata
    33181 gtagcggttt ctcctacagg ttcaggtaaa acactggctt atctttggcc tctactacta
    33241 aacgtggaac caggtgaagc aagtgcgttg gtcatttttg cttcttctca agaactagct
    33301 attcaagtag cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa agtccaatct
    33361 ttggtgggcg gagccaacgt caaacgtcag attgagggtt tgaaggaaaa accagaaatc
    33421 ttgattggta caccaggaag aatattggaa ttgatgaaag caaaaaaaat aaaagcacat
    33481 caagtgaaaa caatggtatt tgatgaagca gaccagctat ttgacgctgg gaacagccaa
    33541 atcattgatc aaattcttca tcaagctcca acagagtatc agctagcttt tttttcagcg
    33601 acagcagatc gttcccttga aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt
    33661 gacgtgacag cagaggacga ttctcgaaaa ggactgagac attacttttt acgtgtaccg
    33721 atacgaaaga aagatgacta cttgagacgt ctcacgcata tagaagattt tcatggtctg
    33781 attttcttta atcaactgac tgaattaggc gtaatggaag aaaaattgct gtatagaggt
    33841 gtaccggtag gaagtctggc ctctgatcag aacaagctac tccgaaaagc agcgctagaa
    33901 caattcaaaa agaaaatggt cagagcactt ttgaccacag atgtggcggc tagaggttta
    33961 gatattacag gtcttcctta tgtagtgaat gcagaagttc cgttaagtga agaagcttat
    34021 ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat tacactcgta
    34081 caagataata atctaagaga tttaaaacgg cttttacgtc catcaaatat aacacttgaa
    34141 gagatttttt tatacggagg taagctgcaa actgagcagc caaccaaaga agatgcagct
    34201 cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa aggagcagaa cccagaatat
    34261 aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa aaaaagagag aaataaagga
    34321 gcaaggcgca aataagcgcc tttttatgct atgattatga aaacgggaac aaaattggtt
    34381 gccagaggag gggttatgtg aaagaacgtc aagaagagat cttgcagcta ttgacagaaa
    34441 accaacaagg attgacagca agtgatgtag ctgagcgctt aactatcgat cgaagcaatg
    34501 caagccgata tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc gctggccgac
    34561 cagtcgttta ctctttgcca acggaaaagt caaaatctga tgaagtgcat gtcgactctt
    34621 ctacgcaagt gacttttgaa acactcgttg gagaaaatga ttctttaaaa gtcagcatac
    34681 agcaggcaaa agctgcgatt ctttatccac cgcgggggtt gcatacgatt atttttggag
    34741 aaactggtac agggaagtcg atgtttgctg aatgtatgta tcattttgca atcgattcag
    34801 agatgctttc tgcagatgcg ccatttgttt ctttcaattg tgcggattat gctcaaaatc
    34861 cgcaactgct ctttggacac atctttggga tcaaaaaagg ggcttacaca ggagcagctc
    34921 aagatagtcc cggtctaata gccaaagcag acggcggtat attgttttta gatgagatcc
    34981 accgtttacc gccggaaggg caggaaatgc tgtttacttt cattgataaa ggtatttacc
    35041 gtccgcttgg agaaagtgca caagtacatg aagcatctgt tcagatcatt ggggcgacga
    35101 ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg gattcctatg gcaatcacct
    35161 tgccgaatct tgcttcacgt tcactggatg agcgttatga aatcatctct ctttttatca
    35221 aacaagaagc gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg attcttgcct
    35281 tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg aaactagtct
    35341 gtgcaaaatc ttttttacat taccgtactc atcatgagaa aaagctaatc attcgaaaag
    35401 aagaactttc ccttcaagta caaaaaggat tactgaaaat caaggaaata ccggaacgtc
    35461 tcgaccgatt catggacagc aaaagtcagt atttgacatt tgaaccagga tttgctgatg
    35521 ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag
    35581 tcacttctct ttccgaaacc ggtgtggaga acattgatct agagaccctc atttcaaaag
    35641 atgtggatgc ttatttccag acatatgtca aagaattgac taaaagttcg attcccaaag
    35701 atcttttgcc agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac
    35761 ggttagaccg tatttataat gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta
    35821 cattggatcg tgtgagagaa ggtcatatga tcgtccaccc agatttgaat aacgtacgga
    35881 aaaattttaa gagggaattc caggtagcaa tcgatctttc aacgatcatt gaagaagaag
    35941 aacaagttga gattcctttt gatgaaatcg gctttattag tatgtttctt tctatcaatc
    36001 taggagaaag tgagcctgtt aaagaaaaca aagtagatgt tctcatattg atgcacggca
    36061 gacagactgc ttcaagcatg ttggagatgg cacaagaact attaggaaca aaagtaggga
    36121 aagcttttaa tatgccactg gttatggaag ttcaaacaat gtatgaagaa gtgttacaat
    36181 atgtcaaaaa ctatcaagag catttgacga atggcctact cttgttaaca gatatgggat
    36241 cattaaacac cttcagtgat ttgatttatg aagagactgg cattcggacg aaagctatca
    36301 cgatgacaag taccatgatt gtgatagaag cgatacggat ggcaagtgtt ggaagaagct
    36361 tagaagatat ttatcaaaat atccagcttt cctttgaatc gatcgtacgt gatcagttca
    36421 aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca tgctttactg
    36481 gtgaaggagt agctgccaaa ttatatcagc gtattatgcc agtgattaat caaaataagg
    36541 tagaactgat ccagatgcag tttttagaac gtgaatcatt caaaaaacac atcgatgcct
    36601 tattggaaga atatgagatc aaagcaattg cgggaacagt tgatgttgat tatcaaaata
    36661 tcccattttt ttcagcctat gacgtttttg atgatgaaaa actaaatatt ttaaagcgta
    36721 tagctgatga tgagatcccg acagctaaaa ttgtcgattc acttgctgga tcattaagcg
    36781 atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa gacagttcag cagatccaga
    36841 ccgatttgtc gattatcgtg gaacctggag tagatactgg aatcgttatc catttagcct
    36901 tcatgttaga cggattgatc aaacaagaaa gccatcggca atttgagaaa ctgtctattt
    36961 tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc aaacttgatg gcactagaac
    37021 gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa atgtttcttg
    37081 aaaataaaat aaaaaaatga acacactaat catactgttt acacactaaa catctgtgtg
    37141 cgcactataa aaaagagcta aaaagcatga aaacgctatt tcagctcttt tttttattgg
    37201 catgcttctt gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa
    37261 aaaacaatca tgttagtttg ttcagcaggg atgagtacga gtttattggt cacaaaaatg
    37321 caaaaagcag cagaagaaaa agggatggaa gccgatattt ttgcggtctc tgcctcagat
    37381 gcagataata atttagaatc taagaatgtc gatgtattat tattaggtcc tcaagtacgt
    37441 ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag gtattccatt agatgtcatc
    37501 aatatgcaag actatggcat gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta
    37561 atgaaataaa tcaagtatgc agttgggggt ataacaacgt ggaagatcaa caaaatctag
    37621 aagccatcat ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca atggaagcga
    37681 ttgcggctgc taaaatcggt gattttgagt tagcggatag gaaaatcgct gatgcagaag
    37741 aatcacttat acaagcgcat catgcacaaa cagacatgtt gacacaagaa gcacaaggaa
    37801 atcatatgca agtgacatta ttgactgttc acagtcaaga tcacttaatg acatctattg
    37861 cgtttactga tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac agcgaataaa
    37921 tacaacttct tttttgaacg gaagaagaaa aatttttgaa cggatttttt attttgttat
    37981 aaggagaact ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc
    38041 aagttggatg gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt
    38101 agttgctttg cgtgatgctt ttatcggcac actgcccgca acaatggcgg gatcagtcgc
    38161 tgtaatgatc aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga
    38221 cagtttagca gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg
    38281 ttttgtctgg aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta
    38341 taatcttgcc cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac
    38401 cttgattcaa ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc
    38461 ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac
    38521 actgtcagtt ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt
    38581 tacagttatg atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa
    38641 cattacgatc aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat
    38701 tttaccagcg acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt
    38761 agctgatggg caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg
    38821 attatctcaa gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt
    38881 ctttggtatt catggaccaa atgtattggc accagtgcta gagggaatct ggggacaagc
    38941 acaactaatc aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc
    39001 cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc
    39061 ttttgcatgg tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt
    39121 ctctaaacgt gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa
    39181 tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc
    39241 attcttgatt gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct
    39301 agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt
    39361 agcaacagga gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt
    39421 cttgatttgg gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca
    39481 acaataagat gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc
    39541 gaggaggaac tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat
    39601 tgttagaagg atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca
    39661 agctgttctt tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac
    39721 taatcaaagc acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa
    39781 tggtttaaaa agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg
    39841 gttctttgat cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta
    39901 tggtcacagg tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa
    39961 gagaagaagc aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc
    40021 atcgtttttt tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg
    40081 caaatcagta actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa
    40141 taaccgggct cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac
    40201 atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac
    40261 ctgtgaggtt cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga
    40321 tatgctttta ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga
    40381 tatgatcgaa gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg
    40441 aagtaagcta gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc
    40501 ttttttgtag gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg
    40561 attgaggaaa tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg
    40621 cgagaacgtt ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag
    40681 gatcggctaa caagtcaagt catggcgacc ccttttccag tgaattttta cggacaagag
    40741 tacctaatgg ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg
    40801 atcaatcaga tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct
    40861 tatctggcgc cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat
    40921 aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa
    40981 ataaagcgta tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg
    41041 ccggaaaacc aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag
    41101 caaaaaggca attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat
    41161 atcgtttatc agcttgcaac gtcttccttt attattgcgg agttgggatt tttggatcat
    41221 gatgcttttt gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt
    41281 gaatattcat gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct
    41341 tctgtaatga tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa
    41401 aattatccat ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat
    41461 gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca
    41521 aaagtagaac aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta
    41581 ttattagggt atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa
    41641 gaatatgaag aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat
    41701 ttttgataaa aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg
    41761 catttgaagg aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt
    41821 cttttgtttt agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc
    41881 gatttttttt agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca
    41941 ttggaaatga cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact
    42001 agtggaaacg ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa
    42061 aaaacaaaca agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc
    42121 aatcgcctcc taagcgatag gtcgctggtt caattccagt caggaacgta caaataaccc
    42181 ctgtatcctt tgcgatacag gggtttgttt tgtgtttgtg ccaaaaatgt gccaaactag
    42241 ttatcatttt tatataaact atcgaacaca gcattgattt tttctttatc ctgatcttca
    42301 agctctcgaa caatatgaag ataagtagac attgtagttt ctaaactgct atgtcctaaa
    42361 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgtgtatgc
    42421 cttagtccat gcaaagtaaa ttgcttttca atccctaatt cttttaattt tttgcgcaaa
    42481 tatttgctga cggcgtttga tgaaactaat ccatttttta agttgaaaaa aacaaaatta
    42541 tgagggtttt taacttcaaa attctcgaat aattcttttt gattgatttt aaactttttt
    42601 aagagatcaa tcgtatgttt atcaatggaa attttcctat ttgaagtttc gttttttgtt
    42661 tttccccatt catttaattt ataattccat gttttattaa tagtgatgat ttgatcttcg
    42721 aaatcgatat cttcccatgt taatcctaga agttcagcaa atcgcattcc agtagcacca
    42781 gcaactaaca cgagcatagg agaagagtac tgcgcagaaa ggttttcttc tacgagtttc
    42841 attaaagatt taaattgatc ataatctaaa tatttatctt cttttggctt caatgaatct
    42901 tttcctttga ttactgcttt tctagttgga tcaaacggaa ttaatccttc ttctacggca
    42961 tctttcaacg atgctctaat atggttatta aacttaataa cggtggattt tacatggtct
    43021 ttcgcatact tatttaaaaa gcgttgatat ccaactctat ctaaatcaga aatcaatacc
    43081 gctggcatat atttctttat gttcattaac gtatcttcgt attttctata agtgatagga
    43141 gatactgttc cttctttata aagttgcatc caatcctcaa agtaatctga taataataga
    43201 tttttgcgat cccctttaag gcctttgttc aagttatatt ctaattcgtt ggcggcatct
    43261 tttgcttcgc cttttgtctt aaatcctgat tttcttatct tcgtgtattt tccgtcatct
    43321 tttttgtatg aaatttcgta ttgccaacta tttccacgtt tgactaatct tgccataatt
    43381 gaatcagctc tctttctctg atacaatagg aactataaag aagcctactg tataggtttg
    43441 ttttttcata gaacacgctt gctttggacg gtgggcgtgt ttttatatca attagttatg
    43501 aagagcaatt gaaactgtct ctgttccaaa gtaacctgtt tgtgcttgca atacattatt
    43561 ttgtgcattt gcttgggctt cagagatatc aaaaactatt ttaccagttt gttgcatatc
    43621 aggatttaag ctttccataa agaaagagtt agtgatatta ccattttcat cttgatttgc
    43681 cgacatagat gcagccgaat cagcttcgaa agtttttcca tctgctttta atttgaagaa
    43741 tgagctatca acagtcacag ccttatcacc tgcatttttt acagataagt caactactaa
    43801 gaaagtgtct tttgcgttag taggaagtac agatgggcca acttgtttta ctacttcaac
    43861 ggagtttact ttgtattcca ttttccctac agcaacgtca tcaccgattt tataagtctt
    43921 ttcttctttt gctgtagttt cttttgtagt ttctttatta gtacttgttg atgttgcagt
    43981 agctgaatct ttgttttctc cgccacttag agcaccacca ataccaaaaa acaaaagtaa
    44041 tattaatacc caaaaccaca cttttttgta aaaaggttta ctaactttat acattttccc
    44101 atcttgaccc ataacttttt ttgccattta aatattcctc atttcttgtt ataatatatt
    44161 tgtgatctca gaaatgaggt atgagtccgt gttgcagcac gggctttttt tactgtgcat
    44221 aagagtattt tttcttgaaa taagactggc aaacattaaa acattctgtt cttaacttat
    44281 tattgataga atagaactcc atgaaatttt ctaatttgaa ctgagattca tcagtcagtt
    44341 cgttctcaat aaagatattt agtaaaatca taattgcgat tctatcagct tcagtttcga
    44401 attttgaatg aaaagttgta gagttatcgt acagtactga atattcaaaa tgtgaagcaa
    44461 tgaaatgacc gagctcgtgg gctaaatgaa aagcttcaga actgtcttcg tgtagttttt
    44521 cattcagaaa tactattctt ggttttggat aataaaaacc tggttcttcc atttccatat
    44581 agattaactt taaattatac tcactcagca tttctttcaa ctttaaatac atacaaccca
    44641 tcactccaac tattcatttt cctctaaagc tttagcaatt gcaatcgctt tacgcattgt
    44701 ctccttagat atttcttttc catcaaaaga aaaaacagta tcgtcttctg ataaatccac
    44761 atgtttaggg gtttcttttt tatctcttcc tagaagatag tctatagaaa caccaaaata
    44821 atctgatact tttgccaaac cttcggagtt aggtgatact gatttccatt tactgaaata
    44881 tccatttgaa tatcccaatc ttttttctaa ttcccttacg gatattccgt tttgctttgt
    44941 taattctttt attttttcgt atggattcat tgatatgtca acctttctag actgacaaga
    45001 aaaaatatag aataaaactc tatttatctg ttgacaaatt agagttaaac ctctatactt
    45061 gttcttgtaa acaaatttaa caactaaaaa gacaacaaaa aacacttttg attattaaac
    45121 gccaaccgcc aaaaaagctt ttaaaatcaa tgtttatatg tcttatttaa ctatgtacaa
    45181 agtatagaat aaaactctat gctttgtcaa ttgaatttag aaaaaagttg ttaaatttgt
    45241 ttacgaatat aaaagaaagg aatgagaaat atgaacacac cgcaaatttt taatttcgaa
    45301 caaaacgaag ttcggacaat tttagtaaat gatgaaccat attttgtagg caaagacgtt
    45361 gcgagtgttt tgggttattc aaacactaaa gatgctttgt cgcggcatgt agatttggaa
    45421 gataagatgg ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac aatcatcaac
    45481 gaatctggtt tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa aaaatttaaa
    45541 cgttgggtaa caagtgaagt gttgccagca attagaaaac atggaggtta tctaactcca
    45601 gaaaaagtag aagaagcttt gcttaatcca gatacaatca ttcaattagc aactcaacta
    45661 aaagaagaaa gaactggaag attaatcgca gaacaaaaga ttgcagagta cgaacctaaa
    45721 atctcctatt tagatagcat attatcttct acagattcag taacaattag tcagattgca
    45781 gcagattatg ggatgtctcc acaacagatg aataaattac ttcataaact aggtattcag
    45841 aaaaaagtcg gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg atacacaaaa
    45901 tctcatacaa ctgagatccc gaaagccgat ggtggcacta aaattgtaat gaataccaaa
    45961 tggacacaga aagggcgtct atttatctac gaattactaa aaaaagaagg atattaccct
    46021 caaatggatt tagaggaaat tggttagaaa ggagttttag tatgactgac attgcagaaa
    46081 tcactcaacg agatagagaa aaaatcaaag aatatgtcga aagttcaaag ttcttaactt
    46141 acaccatgct cgctgaaaga ttcggcatta gcaaaagcta cttatcttta attttaaacg
    46201 gtaaaaagac ttctgcagaa gcaaatagaa tcatagattc gattattact atgtacgaat
    46261 tgtaaaggga ggaacagcta atgcaatatc tagaagcgaa aatcccagtt ccagaaggct
    46321 atgtaattat ctcccaagtg gattatgagg agttaaaaaa agctgatgat actggtagat
    46381 ggatgacgtt gccagaagtg ctagaacgga ttaacagaaa atatgattgg tttacttcta
    46441 gagttttaaa gaacccaaga tatagaaata ttatcgatat cgaaaaaaac aaaaatggat
    46501 ttgtctatta tccagttgaa ggaagagaca catatctatt tttaagaagt aaaacacttg
    46561 aatttttaga aacaaatttt tcggaaatct taaggaggca agcggatggg aaaatttaat
    46621 agagcgctag tattcagcgc accgctaatc atctacgctt taggactttg gggaagcaga
    46681 caagcgttga taggaacgat tgtttacatg gtttggattt ttatggggct ggatgaagct
    46741 gagtacaaaa caaaaaagcc agtcgggagg gactgactaa tgaaaaaaag tttaatgact
    46801 ataaacgaaa aacaattgaa agaaaaattt aatgatctca tcaaagaatt tgtaaaagaa
    46861 actggagaat ttcctaatca aattcatcta gttgcggagg ggcatagccg gtatcaagct
    46921 gtgaaatttg agatgaagaa acaaatctat tgaatttagc agtacctcta cttgtaagaa
    46981 catttattgt aatgtttttt tcgactaaag attcatcgta tctaaaaacc aacgtagtaa
    47041 tttttgactc atacggtgga atggtaatag gtaatgacaa cggaggaaat tttgatagat
    47101 cagcaccttt ttttaaagat ttaccagaat acgcacgtat accatgtgga agttcgtcat
    47161 acattgattt taccgttaca gaataattat caccaatcat tgtaaaagca tttaaaggat
    47221 caggaattcc agaaatcgaa aactcaataa ttgtaacagg taaagaactg ttatttgtta
    47281 acaatacaga atctataatc ctgaatttat tttgatggta aacatctggt ttggaatcac
    47341 tttctctatc tatcaaccat tcttcgcatt cagcgaagaa atccaatttt aaattaaatt
    47401 ttttattttt tctatctgta taggagatat aaaaagcaaa tgttgaaaat actagagacg
    47461 taataggcaa aataaaatct ttaatggttt taacaatgtt attccagtca aattgtttaa
    47521 taaagtcaat aagactcaat ttataccacc accatttttt atctacatta tatcaaagag
    47581 gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg tgactacgag
    47641 catacaagtg gcagaaagct ttgagaaaaa acatcaacat gttttaagag atattgatgc
    47701 actaaaaaaa gatgtgtcca attttggaca gatgttttct gaaggtaatg agccagattc
    47761 ctacggaaga aatcgacgag ttttcttcat tagcagagat ggatttttct tgttagctat
    47821 gggatttaca ggaaagaaag ctatttactt caaacaaaaa tacattgaag cattcaacga
    47881 aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagaca tgatgatcta
    47941 ccaactagaa gaaatgaaag atgttaaaaa agatgtctcc atgcttaaag atactatgcg
    48001 aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatacgaaag ttatggaagt
    48061 tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt
    48121 ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag
    48181 attcgatgat gctgtttcat ttattgaaat gtggttacca gaaactgcga ttcgcatgga
    48241 aatcgatcaa ctgaacagac aacaaagact ttttggtgat gaaaatgaat agagctgaag
    48301 cgctaagaat cgggacggta attgctaatc gctggtggag acacaataaa ccaatcatcc
    48361 taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag tgactccgcc
    48421 ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata tggaaaaaga
    48481 actttccact ctagatcaat atttgactga tcctagttgg ggcaaatcga atatcaagga
    48541 aacaaataat cgaaaaatca gacgaaatct tttgacggat gaagaactag catgtgatca
    48601 agacgatttg ggaaattttg tgagtatttg ggatcatgtc tatcttatcc atttatcgag
    48661 gaagtccaaa aaacctgaat atatctatgt catcgaagat ggcttgattg atgcgctaga
    48721 agagtatgac agagataact tgattgatat ctcttattac ggatcaggta agaaatacat
    48781 tgctgaaatg gaggcagaat ttgatgagtg aaaacaaagg gacaacgact tttgaaaaac
    48841 tttttagtcg caagttaaat aaaatactca agaaaaaagg aaattttgat tatttatctt
    48901 gggctcacgc atgggagatt atgaaaaaga atgatccaca ggcaacggta actattaatg
    48961 agtataaaca ctacagagtt gtttctggaa ctcatcaaga ctttcttgtt gaggaatata
    49021 aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg gttaaaggac
    49081 acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt gttaaaccaa
    49141 atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg gctctacacg
    49201 gactgggatt atatgtattt caaggggaag atattccaac accacctaga atcgatacga
    49261 agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg ggtaaagata
    49321 tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg ctcttagctg
    49381 ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg gagcgagcaa
    49441 tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac cattaatcga
    49501 ttcatattca gcggttctga aaaagttcaa aggaaaagac ataggtgcaa ccatcaatga
    49561 ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg atcgagttat
    49621 tgaaattcgt tttattgatc ctagacgttt cacagtacag caacgaaact tcatctatgc
    49681 actcataggc gatattttca tcgatacagg catgccaacg gacttctgga aggaattctt
    49741 ttacttccgc tttgaaggtg tcacagggcg caaaataagc ctcaaagatg aatcgaatac
    49801 gactgtgagt gatgctaacg tcttagcaaa tatcatctta gatttcatct ttgaacatca
    49861 tattcctttc aaagaaggct atgagatttt accagcgaat caagaatatt acttctacaa
    49921 atgcatcaca aaaagagtct gttgcatctg tggcaaaaca ggagctgata tcgatcactt
    49981 tgacaaagcg ctaggaagac gaaagcgcaa agaagttgat cattcagagt acacatttgc
    50041 agcactctgc agaatccatc acacggagaa acacaagata ggtgtgatca atttcaaaaa
    50101 taaatatcaa atcaaaggga tcaagttaaa ccaggaaaca attaagaaac ttagaatagg
    50161 aggataaaaa atattggctg acaacaaacg atactactat ttaaaactaa aagagaactt
    50221 tttcgatagc gacgagatgg ttcttttaga aagtatgcca gatggataca tttattctaa
    50281 tattcttctc aaactttatt taagaagtct gaaacatgaa ggcaaactaa tgtttaatga
    50341 caggattcca ttcaattcta caatgcttgc gactattaca agacactctg tgggagttgt
    50401 agaaaaagca gtacaaatat ttcgtgattt acaacttatt gaggtattag ataacggagc
    50461 aatttatatg tctgatatac aaagttttat tgggaaatct tcaactgaag ctgataggaa
    50521 gagaaaatac agaaaagaaa ttgaggaagc aaaacagaat ttaataacaa gtggacaaat
    50581 gtcggacaaa tgtccggaca aaactacacc agagttagag atagagttag agaaagatat
    50641 agagttagag aaagatatag agaatgtaac gccttcgaaa aaatcgaagg ctaagcccgt
    50701 ccgtcataaa tacggagagt ataaaaatgt tcttttgtca gatgagcaga tggagaaact
    50761 caaaatagaa ttccctaatg actaccaaga gcgaatagaa cggctatctg agtattgtga
    50821 atcatctggt aagacttata aaaactattt ggcaactatt cgaagctggg caagaaaaga
    50881 aaaaagtgaa tctaagaacg caagcagtgg atacaagcgc acaggaagac gagagaagct
    50941 tcctgagtgg gcaatcgacc aagaagccta tctcaagaaa aaagcgctag aacgagctaa
    51001 tagacaatca aaagcaccat tctaagaggt gggaaattga aaatcgatta tctagaacta
    51061 attaatgaaa tagcaaagta taaaactggt gaggaaatag aaatcctgag agacgtatat
    51121 gatcaactcg aagaagctgg aatcgaagga attaagaatg atcgttcgag ttggagtaag
    51181 ctcagatact atttcgcact ttatatcgat acaacacaat taagaaattt agcatatacc
    51241 aaattactat ttgttgattg tgttaaaggc ttgcaaaaac atcttaatga acttgagcag
    51301 gtgtaatcag atggacctaa agacatttac agcacagatt gaactaatgc atcaagaagc
    51361 tttaagacaa agtgtatcgt acgaagacaa gtggctcaac acgttccacg gtggacgtga
    51421 gagcgcactt gatcaagtac tcaaattatt gaaaggggaa cgtcgggatg gataagaaag
    51481 cggcttggcg aaaattaatg ttgctgattc aagatgagaa ctggcaagaa gatgaagcag
    51541 tggttgctga agttcagcgt ctagaaaaga ttgctaacgg acgtatacga aaaaagcctg
    51601 ataagcggaa acttcgcaaa ggaaaaatca tcgttgtttt gtatgaaggt aacattttga
    51661 tgcaaggaac ggcaagagag ttgtctgcag aaactgaata tacgagcgga actattcgga
    51721 cgtacgcgtg gcggaatcac gttgataaaa aaggacacga atataagtat ttggagggca
    51781 gcaaatgata ccgaagttta gagcgtggta cacaccattt aaaggtaaaa caattggaca
    51841 agaaatgaaa tatgggcaag caggaaggtt gatcactcat gctgaaatgg ctccagataa
    51901 atatgtgctt atgcaatcca caggactgaa agacaagaac ggtgtgggga tatttgaagg
    51961 ggatgtagtt cttgtcaatg taagtaatgg ttttgatcat ttagttaatg aaaaaacggt
    52021 tgtacaagaa tccgagtttc attctggatt agtttgtaaa tcattaatta gtggaatgga
    52081 atatagagta ttcaaacaca aaaagatgga ttatgaatat gaagtcatcg gaaatattta
    52141 cgagaattca gagttattgg aggaacagcg atgaataaac aggaaacaat caaaaagtta
    52201 gaaagtatta aagcgatagg aaatgatgca atagctgctt gctataacga gagtatgaat
    52261 gcaggtatta cgttaatgaa aaaaatagac gaaccgcaga aacacgttat gccgaagttt
    52321 tttgacaaat gggcaaaaca agtcctggaa aaacgtgaca agttttatgc catttctctt
    52381 attgcacgtg caggttgggg atatggtgtt gattttgaac ttagcgagaa cggattatca
    52441 tcagaaaaca aagagctgtt gtactggctt gttgataaat gcagcgacaa ttatcctaat
    52501 aaaaagaaag caatagaagc tttgttatac ggctacgagg tcgagaaaga accgttgtat
    52561 cacgtgaagc taaaaatacc aggcgtaaca tactacctta tccaaacttt tttaatgcca
    52621 ggagatactc atatgtgttt ctcagcggct acagaacgtt atggggctaa atggaaaaat
    52681 acttttacgg aatcagaaat aaaagcaatt gatgaaagat actggccatt tgctgtgcca
    52741 gtggaagagg aggcagaagg atgaaaaaat tgcaccaact catatctgag aaagaaagcg
    52801 aactccaaaa tttagaagat agtttgggat tggggtttcc aattgttgag caagctaaaa
    52861 tgactcaaat cagtcatcta cgacttgaat tggaagattt aagacaaata gaaaaatcaa
    52921 ttcaactcaa cgacaaccag caaatcgtgt ttgagtggtt gaaactaact gcgccaacag
    52981 gaaagccaat gcaagttgta ttttggatga tgaataatgc agcatggggg catttagacg
    53041 aattaaggga tcctttgatg gaattaacag acaaagagca attcgaagtc ctagcagcat
    53101 tcgctcaatg gggattagaa caggaggaag cggaatgaac aacagacatc gcagaataac
    53161 gaaactaaga aaacaggaac tgaatgtact aaaggcaaag ttcgaaaaag aatacggagt
    53221 ttctgcagaa gaagcatata aagtggcaag tcagtgtgtt gctgatgcga gtaatgctat
    53281 tcgtaagttt ggaatttcga tattaagtga tgatcgtaaa tgggaggata tagaatgaag
    53341 gtttatgtag taaggttcgg gaataaattt tatagaagtg atgaacgctc tataggcaat
    53401 aatgtgttat tccttgtaga ttttcttcaa aacgcaagat ggtttgataa tctcgaagaa
    53461 gctaaccaag tttcacgacg acttggtgga ttaacgcaag tatatgaact ggtcactatc
    53521 gatcatgagg gggtagaaag atgaaactaa aagacggatt ttacgctagt agtcatggta
    53581 tcggcggttt aatgctaggt atgccgacaa agaaccctaa aacacgtaag aaaccaaaat
    53641 tcaaagtcgg tgacatggtt cgctgcgaag cagaggagtt catctatccg tttcgtggat
    53701 atgtagagca tgtttataat cactcagcga tcattcgcat tgaaaacacg atggaatgtg
    53761 acaagcggtt agcgaaaagc aaagagaatt tagcagtggt gagattggtg gatatggaag
    53821 ttataaaatc ttaattttgc ttttttacaa ctatcctaca acaatgagcg ttattccaca
    53881 actggaagga gaagaggcaa ttgtgaacat tttagagaat atagatatta agcaaacaag
    53941 gaaaaatgct agacgattac taaaaagata cagaaattta gaacgtttgg tcggaccagt
    54001 gaaaatagac ttttccgtga tgactgttac caaaaatttg aaattcacaa ttgacagtca
    54061 aaacgaagaa atcattgaag cgataagtac tcgagattcg gttatcgaag cattaacacg
    54121 gctaagtaga atccatttcc aggtacttta ttatagttat tgttttccta ataagatgtc
    54181 aatgtatcaa ataggagaaa aattaggata ttccgataga actatcgaga gaatgaaggc
    54241 agtagctttg gttgaatttg ctgaggcgta taaatcagga gaactcattt cacgtacaaa
    54301 ataaaaaaag tcggattcct ccgactatga ataatatttc cgacataagt attatatcat
    54361 aattggggga atcagaggat ggtacttttc gatgtaaaga aatatgagac accaagcgcg
    54421 aaagatgttg atatggaacg cacaaaacat aatgtcgctg tgtttctttc agcatatcta
    54481 tcagctagat gtagagtagg tcaacctcga gaaccaaaag tgacagcatc ttattccttg
    54541 gttccacctt ctacagctga tcatatattt gaagcagaaa gaatgctgat cgataaagaa
    54601 gaagcacagg aagaatttga gtatctgcat aaattgtttg tccgaggcta ttcagcgata
    54661 cagcatccgc ataagcctga tgtgactgaa aggcgcaaga agatattcta tgatcgttat
    54721 atcaatggca tgtcgattta tgtaactgcc caaagaaata atacgagcga ggaatctgta
    54781 aaagcagaat caaacaaaat catcattcaa tttgcttctt ccttagaact ggttgctttc
    54841 aagtagccag tttttacact ttttataccc ttttattacc aatttggttt ccattttata
    54901 ccttttttat accaatcact tacctattca acgttgtatt atgatagtgt cgaaagatta
    54961 ggaaacagga tcgacaaaat aaaatgtaag ggaggaaatc tccctcatcg tttaattaag
    55021 cttcgatagg cagcaacgga aatattaaga ataaggatgt gaatttcaac tccttctaaa
    55081 ttgttcttat tatctatcat ccgttgctgt ctattaattt atgtattgga gggtaagaat
    55141 atgaaattat cgattgaagg tacttcagga gaaataaaag aaatcctctc agctatttgt
    55201 aatagccgag agattagcaa tttaatcaat atagataatt taaatgaatc tattgttact
    55261 ccaagttccc gagagaaatt gcagaaatct gatcagcaaa aacaactaaa aatgggatat
    55321 tagtagttct ctcgcctatg atggtaacat ctttcagaaa gatagatttg actaccaaac
    55381 tttgctcatc atctttttct atatcatcaa ttttttttga gcgaaattct gcaaaagaat
    55441 ccatcatagc atcaataaga ggatttactt ttacttcagc atcaaagtct ataggagttc
    55501 cgcaaattgt tgctcctcca gtttgaataa taatattatt gttagaattt ttagcgaaga
    55561 gttcaatata ttcaatcatg tctgaacggt aatcatca
//
LOCUS       DAGCWU010000002        54363 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.002, whole genome shotgun sequence.
ACCESSION   DAGCWU010000002 DAGCWU010000000
VERSION     DAGCWU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.002"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..411
                     /locus_tag="MBZ71_000072"
     CDS             <1..411
                     /locus_tag="MBZ71_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4428055.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="MBZ71_000073"
     CDS             509..697
                     /locus_tag="MBZ71_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBT4428056.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="MBZ71_000074"
     CDS             complement(755..1108)
                     /locus_tag="MBZ71_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4428057.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="MBZ71_000075"
     CDS             1639..2061
                     /locus_tag="MBZ71_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4428058.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="MBZ71_000076"
     CDS             2058..2288
                     /locus_tag="MBZ71_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4428059.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="MBZ71_000077"
     CDS             2786..2986
                     /locus_tag="MBZ71_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBT4428060.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="MBZ71_000078"
     CDS             3013..4206
                     /locus_tag="MBZ71_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4428061.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="MBZ71_000079"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="MBZ71_000079"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBT4428062.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="MBZ71_000080"
     CDS             complement(5898..6821)
                     /locus_tag="MBZ71_000080"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBT4428063.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="MBZ71_000081"
     CDS             7017..7622
                     /locus_tag="MBZ71_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4428064.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="MBZ71_000082"
     CDS             7930..8946
                     /locus_tag="MBZ71_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBT4428065.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="MBZ71_000083"
     CDS             9087..10388
                     /locus_tag="MBZ71_000083"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBT4428066.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="MBZ71_000084"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="MBZ71_000084"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBT4428067.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="MBZ71_000085"
     CDS             11117..11515
                     /locus_tag="MBZ71_000085"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBT4428068.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="MBZ71_000086"
     CDS             11581..12663
                     /locus_tag="MBZ71_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4428069.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="MBZ71_000087"
     CDS             12875..14437
                     /locus_tag="MBZ71_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428070.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="MBZ71_000088"
     CDS             14430..15563
                     /locus_tag="MBZ71_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428071.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="MBZ71_000089"
     CDS             15563..16519
                     /locus_tag="MBZ71_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428072.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="MBZ71_000090"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="MBZ71_000090"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBT4428073.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="MBZ71_000091"
     CDS             17808..18626
                     /locus_tag="MBZ71_000091"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBT4428074.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="MBZ71_000092"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="MBZ71_000092"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBT4428075.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="MBZ71_000093"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="MBZ71_000093"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBT4428076.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="MBZ71_000094"
     CDS             complement(20506..21087)
                     /locus_tag="MBZ71_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4428077.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="MBZ71_000095"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="MBZ71_000095"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBT4428078.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="MBZ71_000096"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="MBZ71_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBT4428079.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="MBZ71_000097"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="MBZ71_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBT4428080.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="MBZ71_000098"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="MBZ71_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4428081.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="MBZ71_000099"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="MBZ71_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBT4428082.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27136..27444
                     /gene="rpsJ"
                     /locus_tag="MBZ71_000100"
     CDS             27136..27444
                     /gene="rpsJ"
                     /locus_tag="MBZ71_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBT4428083.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27475..28104
                     /gene="rplC"
                     /locus_tag="MBZ71_000101"
     CDS             27475..28104
                     /gene="rplC"
                     /locus_tag="MBZ71_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBT4428084.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28132..28755
                     /gene="rplD"
                     /locus_tag="MBZ71_000102"
     CDS             28132..28755
                     /gene="rplD"
                     /locus_tag="MBZ71_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBT4428085.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28755..29045
                     /gene="rplW"
                     /locus_tag="MBZ71_000103"
     CDS             28755..29045
                     /gene="rplW"
                     /locus_tag="MBZ71_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBT4428086.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29084..29917
                     /gene="rplB"
                     /locus_tag="MBZ71_000104"
     CDS             29084..29917
                     /gene="rplB"
                     /locus_tag="MBZ71_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBT4428087.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29960..30238
                     /gene="rpsS"
                     /locus_tag="MBZ71_000105"
     CDS             29960..30238
                     /gene="rpsS"
                     /locus_tag="MBZ71_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBT4428088.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30261..30608
                     /gene="rplV"
                     /locus_tag="MBZ71_000106"
     CDS             30261..30608
                     /gene="rplV"
                     /locus_tag="MBZ71_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBT4428089.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30622..31278
                     /gene="rpsC"
                     /locus_tag="MBZ71_000107"
     CDS             30622..31278
                     /gene="rpsC"
                     /locus_tag="MBZ71_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBT4428090.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31281..31715
                     /gene="rplP"
                     /locus_tag="MBZ71_000108"
     CDS             31281..31715
                     /gene="rplP"
                     /locus_tag="MBZ71_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBT4428091.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31705..31893
                     /gene="rpmC"
                     /locus_tag="MBZ71_000109"
     CDS             31705..31893
                     /gene="rpmC"
                     /locus_tag="MBZ71_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBT4428092.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31918..32184
                     /gene="rpsQ"
                     /locus_tag="MBZ71_000110"
     CDS             31918..32184
                     /gene="rpsQ"
                     /locus_tag="MBZ71_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBT4428093.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32242..32610
                     /gene="rplN"
                     /locus_tag="MBZ71_000111"
     CDS             32242..32610
                     /gene="rplN"
                     /locus_tag="MBZ71_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBT4428094.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32647..32955
                     /gene="rplX"
                     /locus_tag="MBZ71_000112"
     CDS             32647..32955
                     /gene="rplX"
                     /locus_tag="MBZ71_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBT4428095.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32983..33522
                     /gene="rplE"
                     /locus_tag="MBZ71_000113"
     CDS             32983..33522
                     /gene="rplE"
                     /locus_tag="MBZ71_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBT4428096.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33541..33726
                     /locus_tag="MBZ71_000114"
     CDS             33541..33726
                     /locus_tag="MBZ71_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBT4428097.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33762..34160
                     /gene="rpsH"
                     /locus_tag="MBZ71_000115"
     CDS             33762..34160
                     /gene="rpsH"
                     /locus_tag="MBZ71_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBT4428098.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34192..34728
                     /gene="rplF"
                     /locus_tag="MBZ71_000116"
     CDS             34192..34728
                     /gene="rplF"
                     /locus_tag="MBZ71_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBT4428099.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34889..35245
                     /gene="rplR"
                     /locus_tag="MBZ71_000117"
     CDS             34889..35245
                     /gene="rplR"
                     /locus_tag="MBZ71_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBT4428100.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35266..35766
                     /gene="rpsE"
                     /locus_tag="MBZ71_000118"
     CDS             35266..35766
                     /gene="rpsE"
                     /locus_tag="MBZ71_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBT4428101.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35781..35960
                     /gene="rpmD"
                     /locus_tag="MBZ71_000119"
     CDS             35781..35960
                     /gene="rpmD"
                     /locus_tag="MBZ71_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBT4428102.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36004..36444
                     /gene="rplO"
                     /locus_tag="MBZ71_000120"
     CDS             36004..36444
                     /gene="rplO"
                     /locus_tag="MBZ71_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBT4428103.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36444..37739
                     /gene="secY"
                     /locus_tag="MBZ71_000121"
     CDS             36444..37739
                     /gene="secY"
                     /locus_tag="MBZ71_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBT4428104.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37801..38448
                     /locus_tag="MBZ71_000122"
     CDS             37801..38448
                     /locus_tag="MBZ71_000122"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBT4428105.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38639..38857
                     /gene="infA"
                     /locus_tag="MBZ71_000123"
     CDS             38639..38857
                     /gene="infA"
                     /locus_tag="MBZ71_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBT4428106.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38890..39006
                     /gene="rpmJ"
                     /locus_tag="MBZ71_000124"
     CDS             38890..39006
                     /gene="rpmJ"
                     /locus_tag="MBZ71_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBT4428107.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39024..39389
                     /gene="rpsM"
                     /locus_tag="MBZ71_000125"
     CDS             39024..39389
                     /gene="rpsM"
                     /locus_tag="MBZ71_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBT4428108.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39417..39806
                     /gene="rpsK"
                     /locus_tag="MBZ71_000126"
     CDS             39417..39806
                     /gene="rpsK"
                     /locus_tag="MBZ71_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBT4428109.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39886..40824
                     /locus_tag="MBZ71_000127"
     CDS             39886..40824
                     /locus_tag="MBZ71_000127"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBT4428110.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40852..41232
                     /gene="rplQ"
                     /locus_tag="MBZ71_000128"
     CDS             40852..41232
                     /gene="rplQ"
                     /locus_tag="MBZ71_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBT4428111.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41503..42342
                     /locus_tag="MBZ71_000129"
     CDS             41503..42342
                     /locus_tag="MBZ71_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4428112.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42318..43187
                     /locus_tag="MBZ71_000130"
     CDS             42318..43187
                     /locus_tag="MBZ71_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4428113.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43177..43974
                     /locus_tag="MBZ71_000131"
     CDS             43177..43974
                     /locus_tag="MBZ71_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBT4428114.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44177..44923
                     /gene="truA"
                     /locus_tag="MBZ71_000132"
     CDS             44177..44923
                     /gene="truA"
                     /locus_tag="MBZ71_000132"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBT4428115.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45256..47925)
                     /locus_tag="MBZ71_000133"
     CDS             complement(45256..47925)
                     /locus_tag="MBZ71_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBT4428116.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48163..48351)
                     /locus_tag="MBZ71_000134"
     CDS             complement(48163..48351)
                     /locus_tag="MBZ71_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBT4428117.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48621..48983
                     /locus_tag="MBZ71_000135"
     CDS             48621..48983
                     /locus_tag="MBZ71_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4428118.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49035..50717
                     /locus_tag="MBZ71_000136"
     CDS             49035..50717
                     /locus_tag="MBZ71_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBT4428119.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            50834..52870
                     /gene="recG"
                     /locus_tag="MBZ71_000137"
     CDS             50834..52870
                     /gene="recG"
                     /locus_tag="MBZ71_000137"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBT4428120.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            52919..53920
                     /gene="plsX"
                     /locus_tag="MBZ71_000138"
     CDS             52919..53920
                     /gene="plsX"
                     /locus_tag="MBZ71_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBT4428121.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54042..54284
                     /gene="acpP"
                     /locus_tag="MBZ71_000139"
     CDS             54042..54284
                     /gene="acpP"
                     /locus_tag="MBZ71_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBT4428122.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        17611 a         9920 c        11687 g        15145 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    26821 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    26881 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    26941 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    27001 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    27061 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    27121 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    27181 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    27241 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    27301 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    27361 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    27421 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    27481 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    27541 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    27601 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    27661 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    27721 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    27781 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    27841 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    27901 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    27961 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    28021 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    28081 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    28141 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    28201 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    28261 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    28321 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    28381 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    28441 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    28501 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    28561 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    28621 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    28681 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    28741 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    28801 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    28861 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    28921 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    28981 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    29041 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    29101 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    29161 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    29221 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    29281 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    29341 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    29401 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    29461 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    29521 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    29581 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    29641 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    29701 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    29761 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    29821 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    29881 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    29941 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    30001 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    30061 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    30121 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    30181 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    30241 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    30301 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    30361 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    30421 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    30481 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    30541 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    30601 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    30661 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    30721 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    30781 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    30841 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    30901 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    30961 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    31021 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    31081 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    31141 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    31201 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    31261 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    31321 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    31381 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    31441 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    31501 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    31561 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    31621 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    31681 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    31741 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    31801 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    31861 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    31921 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    31981 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    32041 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    32101 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    32161 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    32221 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    32281 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    32341 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    32401 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    32461 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    32521 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    32581 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    32641 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    32701 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    32761 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    32821 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    32881 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    32941 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    33001 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    33061 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    33121 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    33181 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    33241 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    33301 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    33361 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    33421 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    33481 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    33541 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    33601 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    33661 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    33721 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    33781 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    33841 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    33901 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    33961 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    34021 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    34081 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    34141 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    34201 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    34261 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    34321 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    34381 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    34441 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    34501 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    34561 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    34621 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    34681 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
    34741 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
    34801 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
    34861 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
    34921 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
    34981 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
    35041 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
    35101 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
    35161 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
    35221 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
    35281 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
    35341 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
    35401 gtagggtttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
    35461 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
    35521 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
    35581 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
    35641 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
    35701 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
    35761 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
    35821 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
    35881 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
    35941 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
    36001 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
    36061 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
    36121 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
    36181 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
    36241 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
    36301 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
    36361 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
    36421 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
    36481 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
    36541 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
    36601 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
    36661 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
    36721 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
    36781 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    36841 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    36901 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    36961 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    37021 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    37081 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    37141 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    37201 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    37261 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    37321 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    37381 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    37441 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    37501 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    37561 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    37621 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    37681 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    37741 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    37801 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    37861 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    37921 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    37981 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    38041 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    38101 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    38161 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    38221 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    38281 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    38341 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    38401 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    38461 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    38521 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    38581 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    38641 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    38701 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    38761 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    38821 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    38881 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    38941 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    39001 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    39061 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    39121 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    39181 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    39241 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    39301 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    39361 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    39421 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    39481 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    39541 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    39601 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    39661 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    39721 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    39781 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    39841 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    39901 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    39961 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    40021 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    40081 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    40141 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    40201 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    40261 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    40321 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    40381 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    40441 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    40501 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    40561 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    40621 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    40681 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    40741 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    40801 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    40861 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    40921 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    40981 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    41041 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    41101 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    41161 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    41221 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    41281 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    41341 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    41401 tatgtttgaa ggcataaaaa acattttaag cgagaaataa ttgaaaattt tgtctttttt
    41461 tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc aattattgaa
    41521 ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt gaaagatgtc
    41581 tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg ttcaggaaaa
    41641 tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat cgtgaaagtg
    41701 ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt tggaatgatt
    41761 tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt tgcttttggt
    41821 cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga tgcacttgag
    41881 aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg agggcaaaaa
    41941 caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat tttagatgaa
    42001 gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat caagaagatc
    42061 aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga agcagctaat
    42121 gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac accaaaagaa
    42181 attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt tcctgaaaaa
    42241 ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat gacagaagaa
    42301 aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact tatcagccga
    42361 atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa gaaaacagtt
    42421 atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag catttgaatg
    42481 cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt cagccggata
    42541 cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt cagtttccag
    42601 aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct aaaaactttg
    42661 gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa gttgggctgg
    42721 atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg cgtagagtag
    42781 caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa ccaacggcag
    42841 gacttgatcc ccagggcaga aaagaaatga tggagatgtt ttggcgtctt cataaggaac
    42901 agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat tttgcagatt
    42961 atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa gaggtctttc
    43021 aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc gagtttgcag
    43081 aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct gaagaattag
    43141 cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt gatatttggt
    43201 cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa attattagcc
    43261 agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta ccttttgttg
    43321 gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt ttttatccga
    43381 ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt cttgtttaca
    43441 agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt tggtgtgatg
    43501 aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac tctactcacg
    43561 cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg tccattgaaa
    43621 gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct tcgttttgtg
    43681 cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg cggtgttgat
    43741 tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct gattccttta
    43801 tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc aagaggctac
    43861 aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag agacacgatg
    43921 gttatcattg cttttgtatt attaactgtg ctactgatct ttttaagagg ataaacgttc
    43981 agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat gcaagcagtg
    44041 tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt tcttttatct
    44101 gaaaagaaag aacgaaaaaa ttttatagaa aaatcattat tccagataac cttttaggaa
    44161 caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcgtacgacg gaacaaattt
    44221 taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag aaaaaacatt
    44281 acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga cagatgccgg
    44341 agtacatgcg atcgggcaag taatccattt tgattatcca gaggagcgtc cgttagaacg
    44401 aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac aagtagaaat
    44461 tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc aatttcgagt
    44521 ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt ttccttaccc
    44581 acttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc acgatttcac
    44641 ctctttttgt gcatcaggaa gctcgataga ggataaaata cgtacgatct acgaggctaa
    44701 gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa atggcttctt
    44761 gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg gccgattgcc
    44821 tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag gacctacagc
    44881 acaccctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggtta tgatagaagt
    44941 ggtcagcttc aagaaataag aaggtattta cgaaaattac ttctcaaaat tttgcaaaat
    45001 ttcggcttat tttcgaagaa gctacttctg gaacaccgtt taagaaagag gtcgtgagcc
    45061 tgtcatttaa ctctgagtca caaagagcaa tttcaaaaaa cagcttctca tctttgatga
    45121 atatcgtgac ttgtggacga ggcgttgata agtgaatcat ctttttgtta cctatttttt
    45181 ctgtaaccaa aaaacgatgg aacgttgttt tgattgccct tggtgcggac agtctgaaca
    45241 ggttccatcg tttctttatt ttctacgtct tcttctgtgt gtgtttcgtg gttgttcttc
    45301 tattgctgga tcaatcgtgc cataaaaggg gcgattgcgg tcaaggtacc agacataaag
    45361 aataaacaat gcaatacata ggacaaaaag gattgcacct atcgtaaatc cttcaggcaa
    45421 gtaactgaaa gtgacagtat gtttacctgc tggaactttt atcatcaaga atgcgtcttt
    45481 gaaattgctg accttgactg gtttcccatc aattttagct ttccatcctt tgtcataagg
    45541 aatagttgtc accaacatgg aatcttcagc ggtatttaca gtcccagaag cttttctgcc
    45601 agtggcttcc atttcgacac ctttttcttt gatctgattc attgcggctt gataggctac
    45661 aacatctaga ccaacgactt taggctccat gaaacttatt tctttcgtcc cataaaaact
    45721 tgcagtaaat gtcactgttg tctcttgagg ataatagcca agatcatagt attgaccaga
    45781 aatgttgatt tgcgtttttc gcgatgtacc attgacagtg atcgtcgcac tagaactttc
    45841 taattgcgca aaattggttg ggaacaaact taagtaagct tgtgtatttg caggcacatg
    45901 gacttcccaa gtgagctctt ttggctggtt atctgctgtt tctgagtaag tgacgccagt
    45961 cgttgtcttt gtgatcgtcg tattcaactg attcttcaaa gtaggtgtat agaatgtaaa
    46021 atactgttca tttagaccag acaatgtatt gatcaagttt gtctgactgg tcaaattatc
    46081 tgtcactggc tgctcaacat cattaacgga caacggtgca gtaaatgcta gtggcaatgc
    46141 attgacattc tgatacagtg tatagtcgcc gctttgacca gcttggcgga agccatattt
    46201 actgaacgtg ctttcgtttt tagaaatgtt atatttgatt cctgtaaacc catccatcag
    46261 caaagtatta ttgttataac ggatattcaa atttgtccct cttgaacgga aacctaattt
    46321 atccaggtaa gaggaagaat tacgatttct tatagaagaa aacaaactga taccactgta
    46381 accatagttg atcgaatcgt ttgcagatac tggatccaga ttttccaagc ggtaaaacga
    46441 gtcggaatct ttttttgttt catttacaag agctttcaag tcaggataag gatcagtata
    46501 taaactgcgg gaagcataat tccaatcatc aagaatgcca tggatcatac tgttcgtatt
    46561 gaccagtgct tcagcagaca tgatcaataa taacaatata ctcagatgat tgaatgaaag
    46621 ttttttatgc tgataaaatc ccacagcaaa agcataaatc cctaagaaaa ccactgtcaa
    46681 tacaaaggaa gtcatcgtca cataggtgta gcttccttca ggtttgatgc cccatgccac
    46741 cgcaaagaga gcagctacga ccaaaatgat tcctaacaac agcccaccat cgtcttgttc
    46801 tatcttttcc catccttttc ctgcaagcag gagcactaag aaagagaaca gataggcata
    46861 acggaataaa aacatattcg gtgcatgcat accatgccag aacaaattca atggaacaag
    46921 ataaaaactt gcgataagta tggcgaaaag actgccgtac aaaaatttat ttttcaacgg
    46981 tactttccga ctggcaaaat aaaacagaca gaggactaat ggtagcagac ctgcgtaaac
    47041 gaacggaatc gatccgtatt ttgtcgtgtc ataaacacca atcatatttt tcatgattag
    47101 atccagataa gaagtcgctt ctgttttcaa cgtagtgatc tgactcagtg cctcgccatt
    47161 tgaccgaaga tccagcactg ctggcaatac aatgatcatt gaagcgccgc ctgccagtaa
    47221 agaagtgata ccataaggaa caatcgtttt tttatattct gaccagtttg tcaatagacg
    47281 ggcgataaaa tataagaaag aaaatacgcc aatcataaaa cccatataaa aactggataa
    47341 aaacaagagt aaataactga taaataaaag tttcggccgt ttaagatcca taacacgatg
    47401 aatccccaaa atcactaatg gaagataaac aaatgcatcc agccacatga tgatctccga
    47461 atgtgcagtg ataaatgaga tcaatgcgta actgacgcta agtgcaacat gcccccagcg
    47521 aggaatcttg aacgtacgtg caagaaacca aaaagccaat ccggcacagc cgaccttaag
    47581 caaagtcaga acatacagtg catctggcat catctggttt ggaaaaaaga acaccagtgg
    47641 cgtgaagatc ccgcctagat agtaagaaat taaagataaa taattcaatc ctagagaagc
    47701 attccatgta taaaaaatac tttgctttcc aagaagaaca tttcggaagc tggcatgaaa
    47761 attagaaaac tgtgaaaagg catcacttgc caaaatactt ctgctgcttc caggatagat
    47821 tccgatactt agataaacaa gtgctaaaat caaaaacgga atcaggaaac tagctgctgc
    47881 gtagcgtccg ttttctctaa agaatgactt taaatttttt ttcatattta ttttccacca
    47941 aatttctatg aagttgtatt cagtttatca tatccttgtt tttctctgct taaaaagagt
    48001 ctagttttca caaaaaaata aaatttagaa taaataactt cataatttgt tcgtataaca
    48061 aaaataaacg caaaaaaagg gtaggaatca aaagtcccac ccttaaaaat ttttattaaa
    48121 taaaaaaccg ttcactatct gaacggtcca agtatcattc aattaaacgc gttcaacttt
    48181 tcctgatttc aaagcacgag ttgacaccca aactttttta ggttttccgt cgaccataac
    48241 gcgaactttt tgtaagttag gtttaacagt acgttttgtt gagttcatag cgtgtgaacg
    48301 gttgtttcca ctttttgtct tgcgacctgt aaagtaacaa acttttgcca tggtaatttc
    48361 ctcctttgcc ttgatttgga gcaatagaat aattcagctc ataccagact aacatatcat
    48421 atagaatggg aatttgcaag tacattttca tagatacaat tgacaattga gaagaataat
    48481 tgtaaattaa acaaggttat acctgaaatt ttgcaaggtt tttgagtact ttgggtaact
    48541 gccttttatt tcaagttgcg ctatgataaa atatgataga agaaaaatgg gagtttttta
    48601 cccgttaagg aggctcattc atggctgtaa aaattcaaac gtcagctgga accattgaaa
    48661 ttaccaatga tgtaattgca acagttgttg gtggcgccgc gacagatgtg ttcggtgttg
    48721 taggaatggc cagcaaaaat caaattaaag ataatattac tgagattttg cgtaaagaaa
    48781 attactcaag aggagtagtt gtccgccaag aagataatgg tattgctgta gatgtttata
    48841 cgattatcag ctatggtacg aaaatctctg aagtttcccg caacgtccaa gaaaaagtga
    48901 aatacaatct agaggcatta ttaggcgtaa ctgccaattc agttaatgtc tttgtacaag
    48961 gtgttcgtgt actgccagac taggcaggag actggctggt aactcagcag tcgatctgaa
    49021 ggaggattat ctaggtgagt gtaacagaaa taagtgcaag ccagttccag gaaatggtcc
    49081 aggcaggtgc gaatcgtctg caagtaaatg cagaatacgt caattcatta aatgtattcc
    49141 ctgtgccaga tggcgatacg ggaacaaata tgaatctatc tatgactagt ggagctaagg
    49201 cagtagtaga ttcaacctca gaaaaagtag gagaattagc agcaatgctt tcaaaaggat
    49261 tgctgatggg tgcgcgcgga aattccggcg ttattttgtc tcaattgttt cgtggatttt
    49321 ctaagcaaat tccagatgtt gtctcattaa atgcttctga tcttgctgca gccttcactc
    49381 atggagttga aactgcgtat aaagcagtaa tgaaaccagt agaaggaacg attttgacag
    49441 tagctagaga agcagcgaaa gctggagaaa agaaagcaaa aagtactgat gatgtcgttg
    49501 aagtaatgac agctgttgta aaaggagcaa agcgtgcttt agctaaaact ccagacttgc
    49561 ttccagtcct aaaagaagtc ggtgtcgtag acagtggagg gcaaggactg ctgtttgttt
    49621 atgagggctt tttaagtgca ttgaatggag aattccaagc ggatgaaaca tatgaacctt
    49681 ctccagcaga aatggatgat atggtcaatg cagaacatca tcgcagtgtc caaggtcaaa
    49741 tggcgacaga agatatccat tttggttact gtacagaaat catggtaaaa atcggagaag
    49801 gacctacagt cgatagtaca ttcgattacg aagaattccg taactattta gatggtatag
    49861 gagattcttt acttgtggtc aatgatgacg agatcatcaa agtccacgtg catacagaaa
    49921 acccaggaga agtcttgaat tatggacaaa aattcggttc attagtcaaa atcaaagtcg
    49981 acaacatgcg cttgcagcat gagacgattt tggaacacga tgaagaagtg aacgagtttg
    50041 cgaaaaaaga aataccacgt atcccgtatg gtatcgtagc gatcgctgct ggtaaaggcg
    50101 tccaagagct gtttgaaagt ttaggcgcta attatgttat cagtggtgga caaacaatga
    50161 atccaagtac ggaagacatt gttaaagcga ttcaagaagt aaacgcagat aaagtgattg
    50221 tcttgccaaa caataagaac atcttcatgg cagctgatca agcagcagaa gtagcagata
    50281 tcccagtagc agttgttcct tcaagaacga tttcccaagg attgacagct atgctttcat
    50341 tcaatgatca agcttcttta gaagaaaaca aagaaacgat gacagaaatg ttgtctagcg
    50401 ttgtgagcgg tcaagtcact cacgcgatca gagatacctc aattgacggt gtagagatca
    50461 aagaaggaga ctttttgggc atgatcgatg gaaaaatcct gatctctaac ccagatatca
    50521 cagcaacagc gttaagtaca ctagaacaaa tgatcaatga ggacacagag atcgtaacga
    50581 tcttggcagg tgatgaagga acagttgaac aagcaaatga atttgctgag aaactaacag
    50641 aaaaacatcc tgatttggaa atcgagatcc accaaggaga tcagcctgtc tatccttatc
    50701 tattttctgc tgaataattt ctgtgtcaga atatctttct aacacagtat tcattcataa
    50761 aaggccttcg gttagtgatc aggtatgatc gctgaccaag tccttttttt gttaaaggag
    50821 ggaggaaaaa tttatgcgtt ctttagatga tccaatcact atgctggcag gagtcggaga
    50881 aaaaagagca gctagtcttg ccagcttggg catccaaacg attgaagact tactgactta
    50941 ttatcctttt cgctacgaag atattcagga aagaaatcta aacgagatcc aggatcaaga
    51001 aaaggttact ttgaaaggaa tcgtagtctc gcctcctgtg atgaatcgat ttggttataa
    51061 aaaaagccga ttgcaatttc gtatgatgca agaacatgat gtgttcaacg tttctttttt
    51121 taatcaacct tatctaaaag aaaaagtcat tttatcggag gaaatcgccg tatacggtaa
    51181 atgggatgca aagcgtaaag ccttgaacgg gatgaagatt ttaggttctc aaacaatgga
    51241 tgacttttcg ccgatatatc acgtcaacaa atcaattcgt caaacgacat tagttgatct
    51301 aatcaaaaaa gggtttcaag aatacggcac attgattgaa gaaaatctgc caaacaacct
    51361 tgtggagaaa tatcgactgc ttgaccgagc ttctgctgta agaagtatgc attttccaaa
    51421 agaccacgaa gaaaaccatc aagccaaacg acgtgtcgtt tttgaagagt tctttttatt
    51481 tcaaatgcgt cttcaaggat tgaaaaaagc agaaaaagca gaaacgaatg gtttggagat
    51541 cctttatgat gtccaaaaat tgaaacaatt tactcaaaag ctaccttttg aactgacaaa
    51601 tgcacaaaaa cgtgtaacga atgaaatctg tcgggatctt cgcagtccgc agcatatgca
    51661 gcgacttttg cagggagatg taggtagcgg aaaaacagtg gttgccgcga ttgcgttata
    51721 cgctaccgtt acagcaggtt tccaaggtgc gttgatggtg cctacagaaa tcttggcaca
    51781 acaacatatg gaaagcttgc aacagctgtt tgatcccaat gaagtaacca tcgcattgat
    51841 tacggggtca acgaaagcaa aagaacgacg tgagctcctt gaaaaactag agcaagggga
    51901 aattaacgtc gtgatcggca cacatgcatt gatccaagat ggagtcgaat ttcagaattt
    51961 aggtctagtt attacggatg agcagcaccg ttttggcgtg aaccagcgga aagtacttcg
    52021 tgaaaaaggc tggcgaccag atgtcttatt tatgacagcg actccgattc ctagaacatt
    52081 agctattaca gcatatggtg agatggatgt atctatcatt gatgaacttc cagctggacg
    52141 tatcccgatc gaaacacgct gggtaagaac acctcaattg gacagtgtac tggattggac
    52201 gaaaaaagaa ctggcaagag gacatcaaat gtatgtcatc tgtccgctga ttgaagaatc
    52261 agaagcatta gatgtaaaaa atgcagtgga aatctatgaa aaacttcgtg aactatttgc
    52321 accaaaatat gaagttggct tgttacatgg taaaatgaag aatcaggaaa aagaagtaat
    52381 catggaagcc ttcaaagaca accagatgca gatattagtt tcgactactg taattgaagt
    52441 aggggtaaac gtgccgaacg cgaccgtcat gctgatcatg gatgcggatc gatttggatt
    52501 ggcacagctg caccaacttc gtggacgtgt tggacgcgga agtgatgctt cctactgtat
    52561 cttagttgct aatcctaaaa atgaactagg tgtggaacgg atgaaaatca tgacagagac
    52621 caccaatggt tttgtactaa gcgaaaaaga tctggaatta cgtggtcctg gtgaagtatt
    52681 tggcttccgt caatctggtc tgcctcaatt tgcgattgct gacttggtga cggatggaaa
    52741 tgttttggag gtagccagag aagaggctgc agccctttgg caaataaagg attggcagat
    52801 gcttcctgag tatcagcctt tagcgcaaag cctccaaata gaaaatcagg ataatcgtta
    52861 tttcgattag cagactgtgc tatactttat actgatgacg gaaaaaggag tggaaacaat
    52921 gaaaattgcc gtagatgcaa tgggcggaga tcacgcacct caagcaattg tagaaggcgt
    52981 ggctttagcg cagcaagatt ttccagaagt agaattctta ctttacggaa aagaagcaga
    53041 aatcaagaaa tacttaacaa atgaaaaaaa catcacgatt atccatacag acgaaaaaat
    53101 caatagtgat gatgagccag taaaagcaat tcgacgaaaa aagactgcat caatggtcct
    53161 tgctgcacaa gcagtgaaaa acggtgaagc agatgctgta ttttcagcag ggaatacagg
    53221 cgcgctacta gcagcagggt tgttcatcgt aggacgtatc aaaaatatcg aacgccctgg
    53281 cttgatgtct accttgccag tagtagggaa agaaggtgcc ggtttcgata tgctggatct
    53341 tggcgctaac gcagaaaaca aaccagaaca tttgttgcaa tacggtattt taggttcttt
    53401 ttatgcacgt aaagtccgag gtatcggacg tcctcgtgtc gcactcttaa acaacggaac
    53461 agaagcaaca aaaggtagtg aagtgacgaa aaaagcctat gaactccttc agaatgaaac
    53521 aagcttgaat tttattggaa acgtagaagc tcgtgagcta ctcaatggtg tggctgatgt
    53581 agtggtcaca gatggattta caggcaacgc tgttttgaaa tctattgaag gaacagcaat
    53641 gaatatgatg aatctattaa aatctgctat tctaaatgaa gggatcaaag gaaaaatggg
    53701 cgctatgctt ttaaaagacg gcttacgttc tttgaaagca gaaatggatt attccaaaca
    53761 tggcggtgcc gttttgtttg gcttgaaagc accagtcatt aaaactcatg gagcaacagg
    53821 ccccgatgct gttcgctata cgatccgcca aatccacaca atgcttgaga cagatgtagt
    53881 tggacaatta gtcgaacaat ttgaaaaaag cgaagaatag tatttggata tctgaagcga
    53941 tccgttccat tgaaaaaaag gtggacattc ctgaaaaatg ccagtaaaat tagccatggt
    54001 gtaggtaatt gacaagtgta tgattcttgg aggtgaaaaa gttgactcgc gaagaagtat
    54061 ttaacaaagt agcgaaaatt atttcaaatc attttgagat agataccgat aaagtgacgg
    54121 atgaattgaa catcaaagat gatttaaaag ctgattcaat tagtattatg gaattcgttt
    54181 tagagcttga agatgaattc ggtacggaaa tttcagatga agacgctgaa caaattgaaa
    54241 cagtcggtgg cgcagtagat tatatcagca gccatcttaa ataatttcaa ataaaagcaa
    54301 agaaggataa aaagctgcat gcagcttttt atccttcttt ttttaatctt tttgacacgt
    54361 aaa
//
LOCUS       DAGCWU010000003        46938 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.003, whole genome shotgun sequence.
ACCESSION   DAGCWU010000003 DAGCWU010000000
VERSION     DAGCWU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.003"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            364..834
                     /locus_tag="MBZ71_000140"
     CDS             364..834
                     /locus_tag="MBZ71_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBT4428123.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            1056..2159
                     /locus_tag="MBZ71_000141"
     CDS             1056..2159
                     /locus_tag="MBZ71_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBT4428124.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2380..3339
                     /locus_tag="MBZ71_000142"
     CDS             2380..3339
                     /locus_tag="MBZ71_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4428125.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            3509..4189
                     /locus_tag="MBZ71_000143"
     CDS             3509..4189
                     /locus_tag="MBZ71_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBT4428126.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            4279..5313
                     /locus_tag="MBZ71_000144"
     CDS             4279..5313
                     /locus_tag="MBZ71_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBT4428127.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            5327..5662
                     /locus_tag="MBZ71_000145"
     CDS             5327..5662
                     /locus_tag="MBZ71_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428128.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            5646..8126
                     /locus_tag="MBZ71_000146"
     CDS             5646..8126
                     /locus_tag="MBZ71_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428129.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            8182..9741
                     /locus_tag="MBZ71_000147"
     CDS             8182..9741
                     /locus_tag="MBZ71_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4428130.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            9738..11102
                     /locus_tag="MBZ71_000148"
     CDS             9738..11102
                     /locus_tag="MBZ71_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4428131.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            11273..12343
                     /locus_tag="MBZ71_000149"
     CDS             11273..12343
                     /locus_tag="MBZ71_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428132.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            12358..13026
                     /locus_tag="MBZ71_000150"
     CDS             12358..13026
                     /locus_tag="MBZ71_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428133.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            13046..13624
                     /locus_tag="MBZ71_000151"
     CDS             13046..13624
                     /locus_tag="MBZ71_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4428134.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            14025..14936
                     /locus_tag="MBZ71_000152"
     CDS             14025..14936
                     /locus_tag="MBZ71_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4428135.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(15314..16231)
                     /locus_tag="MBZ71_000153"
     CDS             complement(15314..16231)
                     /locus_tag="MBZ71_000153"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBT4428136.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            16433..16870
                     /gene="zapA"
                     /locus_tag="MBZ71_000154"
     CDS             16433..16870
                     /gene="zapA"
                     /locus_tag="MBZ71_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBT4428137.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            16928..17476
                     /locus_tag="MBZ71_000155"
     CDS             16928..17476
                     /locus_tag="MBZ71_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBT4428138.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            17591..19951
                     /locus_tag="MBZ71_000156"
     CDS             17591..19951
                     /locus_tag="MBZ71_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBT4428139.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            20038..20352
                     /gene="trxA"
                     /locus_tag="MBZ71_000157"
     CDS             20038..20352
                     /gene="trxA"
                     /locus_tag="MBZ71_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBT4428140.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            20534..22312
                     /gene="uvrC"
                     /locus_tag="MBZ71_000158"
     CDS             20534..22312
                     /gene="uvrC"
                     /locus_tag="MBZ71_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBT4428141.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            22457..23356
                     /locus_tag="MBZ71_000159"
     CDS             22457..23356
                     /locus_tag="MBZ71_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428142.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            23349..24611
                     /locus_tag="MBZ71_000160"
     CDS             23349..24611
                     /locus_tag="MBZ71_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428143.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            24772..26670
                     /locus_tag="MBZ71_000161"
     CDS             24772..26670
                     /locus_tag="MBZ71_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBT4428144.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(27000..27719)
                     /locus_tag="MBZ71_000162"
     CDS             complement(27000..27719)
                     /locus_tag="MBZ71_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBT4428145.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            28056..28388
                     /locus_tag="MBZ71_000163"
     CDS             28056..28388
                     /locus_tag="MBZ71_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428146.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            28375..30378
                     /locus_tag="MBZ71_000164"
     CDS             28375..30378
                     /locus_tag="MBZ71_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBT4428147.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            30397..30867
                     /locus_tag="MBZ71_000165"
     CDS             30397..30867
                     /locus_tag="MBZ71_000165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBT4428148.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            30888..31472
                     /locus_tag="MBZ71_000166"
     CDS             30888..31472
                     /locus_tag="MBZ71_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBT4428149.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            31487..32473
                     /locus_tag="MBZ71_000167"
     CDS             31487..32473
                     /locus_tag="MBZ71_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBT4428150.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            32463..32774
                     /locus_tag="MBZ71_000168"
     CDS             32463..32774
                     /locus_tag="MBZ71_000168"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBT4428151.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            32796..34577
                     /locus_tag="MBZ71_000169"
     CDS             32796..34577
                     /locus_tag="MBZ71_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBT4428152.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            34570..35946
                     /locus_tag="MBZ71_000170"
     CDS             34570..35946
                     /locus_tag="MBZ71_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBT4428153.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            35953..36588
                     /locus_tag="MBZ71_000171"
     CDS             35953..36588
                     /locus_tag="MBZ71_000171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBT4428154.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            36618..37973
                     /locus_tag="MBZ71_000172"
     CDS             36618..37973
                     /locus_tag="MBZ71_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBT4428155.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(38165..39559)
                     /locus_tag="MBZ71_000173"
     CDS             complement(38165..39559)
                     /locus_tag="MBZ71_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBT4428156.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            39651..40622
                     /locus_tag="MBZ71_000174"
     CDS             39651..40622
                     /locus_tag="MBZ71_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBT4428157.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            40619..41401
                     /locus_tag="MBZ71_000175"
     CDS             40619..41401
                     /locus_tag="MBZ71_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBT4428158.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(41570..41941)
                     /locus_tag="MBZ71_000176"
     CDS             complement(41570..41941)
                     /locus_tag="MBZ71_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4428159.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            42075..42518
                     /locus_tag="MBZ71_000177"
     CDS             42075..42518
                     /locus_tag="MBZ71_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBT4428160.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(42561..43370)
                     /gene="yidA"
                     /locus_tag="MBZ71_000178"
     CDS             complement(42561..43370)
                     /gene="yidA"
                     /locus_tag="MBZ71_000178"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBT4428161.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(43389..44756)
                     /locus_tag="MBZ71_000179"
     CDS             complement(43389..44756)
                     /locus_tag="MBZ71_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4428162.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(44740..45570)
                     /locus_tag="MBZ71_000180"
     CDS             complement(44740..45570)
                     /locus_tag="MBZ71_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBT4428163.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            45733..46170
                     /locus_tag="MBZ71_000181"
     CDS             45733..46170
                     /locus_tag="MBZ71_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBT4428164.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            46246..46866
                     /locus_tag="MBZ71_000182"
     CDS             46246..46866
                     /locus_tag="MBZ71_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBT4428165.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        15429 a         8047 c         9965 g        13497 t
ORIGIN      
        1 agctctttca ctaggacgag acccgaatca ctcgataatt gaccacctgt atgagaaata
       61 gtgatatttg aattgaattt tacttggttt ttgtgtaagc tagtcatgaa aagaactcct
      121 ttcttttggt tggtttagtc actttaacca tagcagaaag gggttctttt ttcatcactt
      181 aacgggtgaa gatgaaaaag taattgtaag ctgccgtgag gcagtttcac atggttttaa
      241 ttaaaagtat gaattattca ggtagaatct ctgaagaata aatcacgtga tcgtgacgat
      301 aaaatacact tacgtcaggt gataaaaaat acataagtag aaaactaaga ggaggaacag
      361 aaaatgaaag aagttcaatt gatcagaaaa agcgaactat cagaaggtgg ttgcaatgca
      421 tgcggcgtag tagaagcaac aagctataca ttaaagcttg gtgccaacaa agcgataatc
      481 tctgagttga cagtaggcgg tcttgtggac agtctggcgc ttgctgaagg ttttattgga
      541 gaagatattt atgagatgtt ttcagaaatc cgtcagttaa aaaaagggga aaactgcata
      601 gaggttcatc atgagagccc gaatgtgaga ttcaaacgtg gtgataatga gatgatcttc
      661 aataatcatg tgtccgatca caccgagcta tatggaatcg tgaatcaaat actaactgag
      721 ctttttggat tgggacctta tgcgttcaag gaagaaaacg gtaatccaaa attaaacgaa
      781 gaatggcaag aaacgattga aatacaaaga aacaacccgc acttatttca ataggcatcc
      841 acagtatttc gtaaaaggga ctttgacacg acttttgtca aaatcccatc gttttttatc
      901 tatttgtgtt ctaaacattt ctttcagagt agtaacaact ggaatgactg aaatgtaaac
      961 agattatttg cctgtatcaa tacggaagag taatataaag tgtgtaaacg ctatcatatt
     1021 attcattcaa ttttttatag agggaaggca ttgaaatgga aaaaacgtat caagcaggta
     1081 caaatgaagg aatagtggat tttatcaata tggaagattt agaaatagcc gcttcacaag
     1141 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
     1201 aagaaaatga acgggcattc aatcatcagc tgattattcc tcatgtatta agagatgtag
     1261 aattacccga tacaactaca cattttgaca aagaaacgct gactgcacca atcatcatgg
     1321 caccagttgc tgctcatggc ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
     1381 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtaca ttagaagaga
     1441 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagatg
     1501 atgggatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
     1561 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaat gggtttacct
     1621 tcccactgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
     1681 ctgtgtacaa atcttccaaa cagaagctta gtccaaaaga tgtagaattt attgctgctc
     1741 attctgattt gccagtctat gtgaaaggtg tccagtcaga agaagatgtc tatcgttcat
     1801 tagaatctgg tgctggcgga atctgggtat ctaatcatgg tggccgtcaa ctagatggtg
     1861 gtccagcagc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1921 tcgtgtttga cagcggtgta cgacgcggac agcatgtttt caaagcaatc gcatcaggtg
     1981 cagatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     2041 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     2101 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     2161 aaacaaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     2221 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     2281 tacttcgccg attgtaaaat taagctagac aactaaaaaa agtcatttgt gctacactca
     2341 aaatagttcc gacgaaagaa ttataaggag tgagtacaaa tgacctataa ccatcttaca
     2401 ccaactgaac tcgtaatgat agaagcttat ttcaatcaat cacaacctgt ttcaaaagta
     2461 gccaatctgt tacaacgttc tcgacaaact atttataagg tctatcgttt tcttaaatct
     2521 ggtggaagcg caatgagcta ctataaacaa tataagaaaa acaaacgtaa ctgcggaaga
     2581 catccgattg ttttacctga tgagcaacag gaatacattc aaaaaaaggt tgttcaaggg
     2641 tgggctcctg atgtccttat tggtcgagca gagtttccta ttgcttgttc ggtacgcaca
     2701 ctgtatcgaa tgtttaaaga taaaaccttt gacactcacg atttaccaat gaagggaaaa
     2761 agaaaaccaa acggtcataa agaaaaaaga ggaaaacaag ggtttcgccg gtcaatccga
     2821 gacagaaaga cagactatac ccagtttgat catgaatttg gtcacctgga aggtgacact
     2881 atcgtgggtg gaaaacataa aagcgcggtc atcacattag ttgaacggct atctaaagtc
     2941 atcattacct tgaaacccga aggcagacag gctaaagaca ttgaaaatag gctgaatcaa
     3001 tggtttcgtt gtgtcccaaa aaacttattt aaatccatca catttgattg tggaaaagaa
     3061 ttctcaaatt ggaaaaagat tagtaatacc aacgatattt caatttattt tgcggatcca
     3121 ggaacacctt ctcaacgagg attgaatgaa cattcaaatg gcttattacg taaagatggt
     3181 ttgtataaag ctatggattt taatggtgta tctgaaacct ttatccaatc ggtggcatcg
     3241 aaacgaaatc atattcctag aaaatcatta cactatcgga caccaatgga agtatttttg
     3301 agttacgtca caaaagatga tttgtctaac ttaatttgac aaacgaaact taataaaaaa
     3361 ggaagaatat tcatgtaaac gattttattg tttttttctc ttttatacaa aaagggtcat
     3421 aatttcaagt aagcggatga attcatgagg attttttgca tactgtctct actatactta
     3481 ggtcactaag aaaaaggagt gatttattat gaaaaagaac atgattggga cattattact
     3541 ttcaacactt cttctaggcg gcttggcaac accagctttt gctgagggtc aagcgacttc
     3601 aaaaggagat attaccttta ctgaaccaac aaataccgtg gagcctctta accctactga
     3661 tccttcaaaa ccagtagagc cagcagatcc ggaaaatcca gcaactggcc agacaggtcc
     3721 tttaacactg gatgtagttc ccgaattgcc atttggtaca cacgagattg aaagcggaac
     3781 aaaaacgtat caagtagacg cttcaaaaaa cgatacacct tatctccaag tatcagatcg
     3841 tcgaggtgta ggtgcagatg gccaagcaca agggtggaac gtgacagttt cggtttcaga
     3901 ttttgtaaac ggttcacaag ttttgcaagg cgctgaatta gattttggaa catccactgt
     3961 taaaagtacg tcagataacg aatccactgg accaactagt caaactgtaa ctgggttgag
     4021 taaagcaagt gcagcgacac caatatttac agcagcaaaa gatcaaggtc tgggaacatg
     4081 gctttctgtt tatgatccag caaatatcac actgaaagtg ccgaaagcag cagctggaac
     4141 attcacggct gatttgactt ggaacctcgt ggctggccca gtagcataga gtatgaaata
     4201 aattcaacaa actgcggtga aaatactata ggtgaaggat tttttaccgc agttgttgtt
     4261 gaaaggatgt ggtggacaat gaatcaaagt aaacgactgt ttgtttcgtt tttttctatt
     4321 ttaagtctat ttttcatttt tccgtcaatc agccaagcaa aggattcagc aggagacttt
     4381 ggaatcaaac cggtatttcc agagaaccaa atagataaag caatcggtta ttttgattta
     4441 ttagtggcac cggaacaaaa tcagacacta gaagtaatta tcagcaattc ttcagatgaa
     4501 gaacgaacat ttgaggtctc tgtcaatccg gcggtaacga gtgatggtgg aacgatcgat
     4561 tatagtcaaa aaaacccaac gttagatgag acattgccgt ttgatgtccg tgacgttttg
     4621 ctcattgcga aaaaggaaat caatgtatca gcacatgcag aaacaactgt acctatagaa
     4681 gtgaaaatcc cagcaaaaag ctttaaggga cgtgtattag cgggtattca tgtttcacct
     4741 aaagaagaag ctgaaacaga aaatgctaca gaaggagcac aaatcaaaaa tcgtattgct
     4801 tataatctcg cagtcgtttt acaagaaagt caagagacga tagaaccgga tctgaaactt
     4861 ttatcaggag atttggacga ggtcaacgca aagccaactg ttcagcttcg tttccaaaat
     4921 cctcagccta ggattatcag caatttgata tttacgagca agatctttta tgaaaatcag
     4981 ctttatattg aaaatacgtc taatgcattt ttagttgcac caaattcgaa ttttcacttg
     5041 aatcttgatt tagctggtga taaagcaaaa gccggcgatt accgcgcaga aatcatagca
     5101 aaatcaggcg acagtaatga atggcgattt acacaaaact ttacgatcaa aaaagaaaaa
     5161 gcacaaaaag taaatgaaaa ttccgttttt gcagtacaag aacaatcttt cccatggctt
     5221 tatgcaggat tggttgctgc agttctagtg ttatggttga ttttgctgtt tttgtgggtc
     5281 cacaagaaaa agaaaaaagg agaaacaaca tgatgaggtg atttcgttga aaacatcgat
     5341 cttattggga gggattattg caagtttgct gttcttcagc tggcaagagc cagtccaagc
     5401 agataccaaa agttcaggta cgattacatt ttatgatcta agtccaattc cgcatccaag
     5461 tggttcagtc aatggcgcat caaaacagca gacacagcta ccgatttata aagaatctaa
     5521 tcaacatggc gtattcccgg agacgaatga acaaaagatc cgttttcttt atttgatcat
     5581 tggtatagaa ttatggcttc ttgcgtggtt gttgttatgg agaagaaagg aaaaggagga
     5641 tgaacatgtt gctacaaaat aaattttcat tttttcgctc cttctttttt acttcgcttt
     5701 ttgttttaag tatatttatc ttttcacctt cggcagaagc tgttggagca acgcctcctg
     5761 ccatttctta tgatccatac acgtatgaaa atatcgtacc tccaaatggt ggacgtagtg
     5821 cagtcaatgg cgggaagctt gtccaactaa ctggggctaa ccaattgcct aaattgactg
     5881 ctacttattt gaacgatggt tcaaatgatc cttggaaagg tggcacggtc cgttttcatt
     5941 gggaaagcca tgtgaaacta acaaatgtac ttgatttggt gaatatcact gcacaaggat
     6001 ttgcatttgt ggcgatcatc aatttgccgg aacatgtatc aagcagtgat gtattaaaag
     6061 caattgactg gaacaacgcc tacttgtata ttgccggtgc ccccatcaag gttactagcg
     6121 gaagtttatt atcagcagga agccatacct tgcgttttgg gatggggtcg tgggatacaa
     6181 atactctatt gacagatatt attgctatta ttacgcgagg cgaattcaac gtggacgata
     6241 tcccgcttca atttgatgtt acagctaatg tcgcgaagat gacagaaaac ggagataaat
     6301 atgatacgag tccaaacaaa attttgacca agggtaaatt acaaccttct atgtctaaaa
     6361 aggccgtttt ttctgttgat ttttatgatt cgaataatat tatccaagat cgtatgttca
     6421 attcgatttt agctccagct gcaggtagat tttatcctaa attaactggc ggaaacttga
     6481 atccaggagc atatgcattt tctgcaagtg cagagataaa tacgtggaat agttacatct
     6541 caccaagtga ttcaacagga aaatataata catttgctga taccacagaa tacgtggatg
     6601 gaaataacca gaaccttcca ggctctacca atagtcggac attgacgatg gggctgactg
     6661 aaggacgttt tgatcagttg ccatttagtc gctttgatcg cgtagtcaac tattttacag
     6721 gaaaaaacgt tacacaagga gcaatactga ctcatactcc agcaagcaca agtggattga
     6781 atcagacagc caatatcgtg tacagtggga aagatgcgca aggcggattg ctttctcctg
     6841 ttgctttaaa ggtattggag cagtacaagc tagacggaac ggttttacca acaaacttaa
     6901 cagccgatag tcagtgggga aagctccttt cttctgcatc gtaccaagtc acggccaatt
     6961 ggtcagcaac tgaactaaaa aagggttcga tccattacag gctttattcg aaaggaaccc
     7021 aaaaattagc gacaggatgg acagatcaac ttttccaaac gatccaatca aataacgggg
     7081 gtgtaaatac agctacttct acactacctg cattggcttc agggcaatat tattttgatt
     7141 atcgcttggt ggacgatgta cttactaaca tgtatccgtc ttttgcgtac aaatggcagt
     7201 ctgaacaagc tggaatcacc actcttcctc aaataacagt ggctaatttt ccatctgtga
     7261 gtgcagatag tcagctaaaa aaccttagca gacaacctga gacaggagat cccctgtctg
     7321 ctctatcagg agatacgatt caagaaaatg tgacattcac gttaacaaaa tctggggatg
     7381 cgataaccga caaaaaaatc acgatctcac ttcctgaaaa tactacttat atcaatggtt
     7441 ccttaaaatt aaatggtaca actatcgcag ataccggcat tcaacaagga gtttctgtcc
     7501 cagcagatct attgagcaaa ataggagata ccattcatct tacttacaac tatcaattaa
     7561 atactgtaga tacttcttta caatccgtca gcatactgac aaaggcagct gtactttcca
     7621 gcaatattac attagcagat ggtgctaagc ttcctaaccc agttgtccaa acatctgcta
     7681 aaacaattct tgtccccaaa caagaattaa cattagttaa tgtaccagat gattttacat
     7741 ttggtaacga tctccccaaa ccgttgaaga caagttatta tgaatcaaaa ggcgattttt
     7801 cttttgatgt gcgggatact cggcttccaa gcacgtctcc ttggcaactg acaggtacat
     7861 tgacttcttt atttaagaat gatcaaggtc aagaattaag cggaacaaaa ttatacttca
     7921 atcattctgg atcaaagcaa ttgattcagc aaggtcaaaa cacgttgatt tatgagagtg
     7981 atggaacagc taaaggagag gttctcgttg actttcccga taccgatggt ttactcttag
     8041 aggtaaatag cagtacgaat gctcaacctg gcgcaacgta tcaaggcatg gtcacatggg
     8101 aactgacagc tgggccaacc agctaagtga ttagaatttg tatggatctg tgataactgt
     8161 cacagatcct ctttttcaag gatgagaagg aaacttctgc tgttaaaatc agaaatgctt
     8221 gaaggagtgg acttggaact tttcagtttg cttgaaaaaa atgaacaacg gcaaatcaaa
     8281 cttttccaac ttttattaga ggaaaaagga gaggtttccg ttaataacgt acttacccgc
     8341 ttatcagttg atcgggctac cttgaaagaa gatctgtttt atttacaagc taacttggaa
     8401 aatatcaaac aattggaaat tgttatccaa gaagggaata ttttattaat cagacatgga
     8461 aatatagcag cagtcgatgt ttattatcat tgttacttgc gctattctac aaaatatcag
     8521 atacttcttt atctgttgga aaatggctca tttgatcggc agaaacttgt catggaactt
     8581 aatatcagtt cggctacttt gtctcgcaga gtgaaggaat taaatgattc tttaaatgag
     8641 tttcatctga aaataaaaag cgggcggctg accggtccag aaagtcaaat tcgttatttt
     8701 tatttccaat tattttggtt tggacgtccc tatcttgaaa acaaaaaaga atacagtgat
     8761 ttatcaggaa acactttttt agatctgctg caaaaagagt ttagttttgc tattacagaa
     8821 gaaggacgat tgaaatttga aatatggctt tccattatga agaagcgaat gcgctgtacg
     8881 aaaaaatcca aaccagatta tagcaatctt cctgttggat tgtttcaaac gacacaattt
     8941 ttattggacc tccatacatt tttgacccgt tacttctttc aatatgcctt tccttggaat
     9001 caaaatgaaa cgttggtttt ttatcttttc atgatcagta actttacatt agatacacat
     9061 aactcgcatg tccaggagtt tttgataagc gaaaaataca aagacgacgt agtagctcaa
     9121 ttgaatcgaa cgtttaaaca atcgatagaa gaatatttct ctttttatgt ttttccagaa
     9181 gagatttcgc aaaagattgc gttgacagtg atgcaaaatc acgctcattt tgcctatttt
     9241 agaggatgga tgaatatttt tggccaagaa ggattaaaac agcggttaac tgaagtagaa
     9301 gataaacaat tcctggtttt atgtgaaaga ttggtcagac aaacatttgc tattgttgat
     9361 tttactgaag tagacgagga aaatgcccgc tttgaattat ttgctcgcta tgcatctatt
     9421 ttacggacgc tgatcaagca gttagaatta cgtctgcaaa ttgcttatga tcttccttat
     9481 gaaaggctaa tggcggatat cgtgaaagaa atcatccagg aacaattgct gaatacgatc
     9541 aaatatgatt tagtcgaata cgagaaagaa aagcagtatg atatcatttt gactagccaa
     9601 ttgaaagagt acccgtcaca gaagtccgct aaagtttttg tattcactag caacgaatat
     9661 aattatgatt tcccctattt gaaccaattt ttaaaagaat gttatttaga aaaattgaat
     9721 ttaaggatga ataaaacatg aaaatcgtga tcattggtgc ttcccatgaa ggcatcacag
     9781 ctgccctaaa tctaagaaaa cttcaaccga aagcagaagt tttactgatc gatgaagatc
     9841 ataaagatgg actaggctat gtttcaaatg gaatcaacct ttatttaaaa ggaaagatac
     9901 gtagtttagc tgaagttgcc cataacatgc ggactcttca agaaagtggg gcaaaactga
     9961 tcaccgaatg gagagtaact gaacttgatc cagacaaaca tcagctcatc ctcgcatcca
    10021 aagaaggaaa aaacgagacc ataacctatg ataaattaat cgtagcgaca ggttcctctc
    10081 ctgtcacctt gtataaacaa attgaagctg aaaatgtcta cacctataaa aatctagtgc
    10141 aatctaagca agtacttgcc gctttaaaag aagcaaaaga agtagtgatc ttcggtgctg
    10201 gttacattgg attggaatta gcagatgctt tgagaaataa aggatacatt attcatctag
    10261 ttgattatat gccgaatgtt ttatcaagat atttcgataa ggatatgatc aacagttttc
    10321 aaaatcagct gcaaactaag caaataaatt tttatccaaa tgaattcttg atcgattgga
    10381 aaaaatcaga agagaaagta gtatccgtac aacttctgtc tcaaacaata aaggcagata
    10441 tggtcatttt ttctgcccaa actcgtccta atacgacatt gttaaaagaa aaagtggctt
    10501 tgtatgagga tgagactgtg atagtcaatg aatatctgca gacaagtgat ccggacatct
    10561 atgcgattgg agatatcgtc ccagtcagtt ttgacaaaaa taaacggcat ctctttttac
    10621 ctttagttac tcgagctgta catatggcaa gagcagtagc gctaactttg agtggtcagc
    10681 caaccgctta cgacctcaga caaaagataa cagcagcagt cattatagac tactttttag
    10741 gaactgtagg gctgacagaa gatgaagcgc cattcttgga acaaaatacc ggttcgtgtt
    10801 caggagaatt tgatctattt ccacaatacg acgaagaaaa caaaacagta aatgcaaagc
    10861 tgatctacca tccggataca ttagaaataa ttggcggaca gctgataagc caagaattct
    10921 tattatcaga tttgaacctg cttgcggata tcgtgaaaca caaaactacg attccacagc
    10981 tagcagttga ggattttgga tttctgtctg aatacacctc tcgttttcac tacctcaatg
    11041 agttagcttt taaagtacta gctaagagag caaatagcaa tagagtagat aaaagactgt
    11101 gagaaatatc acggtctttt attgtgtggg gatataataa atattaatca atcgagaata
    11161 aaaaattcat ataatgaaat agcggaagaa ttttttgagc ataatattcg ccaaaaattt
    11221 atcaagtgat aaaattgttg tataaaatga ttatttcagg aggaggagtt aaatgttttt
    11281 agcattaaat gaaattatgc attcaaagtt acgttatgcg ttagttgcag gggtgatgtt
    11341 tttgatcgcc tacctggtat tttttttgac tgggttagct tatggcttag ctcaagataa
    11401 tcgaacagca gtcgacaagt gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc
    11461 aaatctaggg atgtccatga ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt
    11521 ggcgtatctt gcccaaacac ctggagtcgt gactagtaaa gacagtacag aagaaggaaa
    11581 aatcaatgtt agcttcttcg gtatcgataa aaatcaattc atcatgccca atcttgttga
    11641 aggaaaagct tttgataacg atgatgaggc agtaggcgat attagtttga aagaagaata
    11701 tggtctagcc ataggagata cagttaagtt atctggtagt gacaagacgt ttaaactaac
    11761 tggctttacg gatcatgcca aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc
    11821 ttaccaagaa atccgcttcg aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat
    11881 agttgtcaga ggtaaaatca gtaatttacc tgaagattta gaacagatca aaatttctaa
    11941 attcatcaat gaattacctg gatataatgc gcaagtattg acttttggat ttatgatcgg
    12001 atttttgatc gttattgcgg ctattgtgat cgggattttc atttatgtac tgacgatgca
    12061 aaagattaat atctttggtg taatgaaagc acaagggatc acaggaggat ttattgcgcg
    12121 ttctgtagtg gcacaaacat tcattttatc atttgttggt attcttcttg gtctattagg
    12181 aacagtggga acatcccttg ttctacctga tgcggttcca ttccaaagca attggctgtt
    12241 ttttggcgtc attagcttgt tgatgttagt agtagctgtg ttaggcgcac tattttctgt
    12301 tcggacaatc gtaaaaatcg atccgttgaa ggcaattgga taagaaggag gaagaacatg
    12361 aaagcaatag aatttaaaga agtagaaaaa agtttccaag atggcgacca agtagttaaa
    12421 gcattgaaag aaacaaccat gagtatcgat aaaggggaat ttgttgcaat catcggtcct
    12481 tctggttcag gtaaaagtac ttttttgact ttggcaggcg gattgcagac gcctagcagt
    12541 ggctctgtat ttatcaatga tcaagaattc agtaatcaaa aagaaagcga acgcgttaaa
    12601 ctacgtttta aagaaattgg ctttattttg caagcttcca atctggttcc ttttctgaca
    12661 gtaaaaaagc agttattatt agtagataag attaaacatg aacatcgtca aaaagaagca
    12721 aacgaattat ttgagcagct tggcgtggat aagttgatca acaagtatcc agaagaacta
    12781 tctggtggag aacggcaaag ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt
    12841 attctagcag atgaaccaac tgctagtttg gattcagaaa aggcacgaga agtagtcgat
    12901 atcttggcaa aagaatcaaa agacaaaaat aaagcaacga tcatggttac tcacgataca
    12961 cggctgattg acaaatgcga tagaatattt atgatggaag atggtgtatt gaaagaaaaa
    13021 gaataaccaa aaagcaggtg gaaaaatggt ccatcgatac aacaaagata cacagcaaaa
    13081 aattttgaaa gaagcgactg acttgtttat gtccaaggga tatctcggca cttcaacaag
    13141 agaaatcgca caaaaagttg ggatcaccca gcccaatctc tatcattatt tcggtgataa
    13201 agaaaaacta tatacggcag ttttagaaaa ccacttaaaa gatgttggaa aagctttgag
    13261 agaaatcgtt caaacaagcg aaacgggatt tcaagtaacc ttgacaaaaa tggcgcagtt
    13321 tttgattgag acacatctgg tcgatttgtt tctgatgctt cacgatcttg aaagcaatct
    13381 ttcaaaggaa acaagagatc atcttttctt tctatggaag aaaaattatc gtgaaccttt
    13441 tgaagaaatc ttttcagcaa accaatctgt gctaagaaat gggatttcaa aagaaatcgc
    13501 agcaaggcat tttttcttag tgttagcacc ttacatcact cggtcgaaaa gacctgtgga
    13561 agaaccgcta aatgtggcgc aattgatcga tttatatttg aatggtgtat taggagaagc
    13621 gtaaagctgt tatataggga gaaaatagtt aggtgaagaa gcaagatatg acgaagatcg
    13681 ttatatcttg cttctttata taggtagttt ttcataaata caaatttgtt attctctcat
    13741 gattatcagg gtatattttt atttattatg tcggtgttcc aggaagtgtt ttaatttaat
    13801 aacgaagaaa ttaattgttt gtagaatgaa ggaaactatt tattgggtag atgaatcacc
    13861 acgaatagtc aaaaggagat ttcaggatat gccaaaaatg aaaaaaatca ttttagcagt
    13921 aatgttattt tctacggtta tatcttttac agcccctttt ttgcttactt aagctgattt
    13981 taagttttgg cccttagtct ggatctacga ggtgagagtc aagaatgaaa aaaataattg
    14041 gagcttttgt atttgcaatc attatcatta ttatctgttt tcaaatagat gtttttggtc
    14101 aaaaaaagat agttggacaa acggtcagca gtacgaacag tacggttaca aagacaaagg
    14161 aatcatttga tgatacagaa caagagacat taatcaaaat cggaaaaaat gaccgtacac
    14221 tatatacaga tgactcgcta actaccccac ttgcaactat aaacggcgga gagctggcgg
    14281 agtttctttc tgaaacaaaa aatgcttatc agatcaagac aaatgatggc tatactggat
    14341 atttagccct cgaagatgga accaaagtca caaagaatat acaaacaaaa ccggaagagc
    14401 tatctgatgc agtgattgta ttagatcctg ggcatggcgg aaatgatact ggggcattaa
    14461 gcaatgatga acaaacagaa gaaaaagata taacattaag tacagccata aaagtaaaaa
    14521 aggcgctaga ggatgctgga gcaacggttt atttgaccca tactacagat gaattagtac
    14581 agcttggaga tatatgcgac tacagtgaag aaaaaaaggc agatgtcttt atcagtttac
    14641 acgcagactc tacggaatat gcgaatgaag caactggaat cacgacatat tattactatg
    14701 gacaagagga aacattagca cagacaattg ctgactcctt cactgatctg cctctggatt
    14761 caagaggaat ctccactggg aactatcaag ttcttcgaga aaatcttcaa ccttctattt
    14821 taatcgagat gggttatatg aataatgata gtgatttaaa ggaattagta acagacagtt
    14881 atcagcagca gattgctgca agtttgacac aagcattgac cacttatttt cagtaataaa
    14941 taagcccagc caaacaaaaa tagctcctca tatttttcgt ttggccgggc ttattatcgc
    15001 agatgcaagc ttttaaacac cgtttaagga agaggtcgtg agcctgccgt tcaactctga
    15061 gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg atgactattg tgacttgtga
    15121 ccgaggagtt ggcatgagaa caccgtttaa gaaaaaggtt gtgaaatcaa cgttttgttc
    15181 tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
    15241 taccgaggat cagatgattg aacaccgttt attcggaaca agggactgtg acatgacttt
    15301 tgtcacagtt ccattatttt tttagtagct tcaaagcttt ttctgtattg gcaaaatgaa
    15361 gtttggcata tttttcaagc ataggcatgc cgcctttctc caatagtttt gccgctactt
    15421 ggtcagattt cgttccagct cctgacggaa gggtgattcc agcttcagct gatgctttgt
    15481 ctaactcttc caaaaagctg gcacgactga tgatagaagc agcagcaaca gctaaatgat
    15541 attgttctcc tttagtaacg aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg
    15601 catatttttt aaaatttgct tccggcgtaa actgatcgat caaaatcgct tctggttttt
    15661 ctggctcgat tttttttagc agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc
    15721 gaacggcatt gtattttggt tggatctcat tgtatttttc tggagataag acaagcagtt
    15781 tataggggat ggtttttttt aagatgtcag caagctggat gatttggcta tcttttaact
    15841 cttttgaatc tctaacacca agtttacgta attctgcaag ctgatcacgt ttcacataag
    15901 cagcacatac tgttagcgga ccgaaatagc tgccatttcc gacctcatca ctacctaaaa
    15961 cggataattg actgatattt tttggcaaag aagaggctgt ttttgctggc ttggcttgtt
    16021 tatcctgtgc ctccccccat ttatcagctt ctattgctgc attcgtacct tgaaacatca
    16081 cttttccgga tgtataagcg gtaatggtcg tcccgtcttt ttttgcagca aagctagtat
    16141 aaggaatctg tttatttaac aaataagagc gatagtatgc tttcatttga ctaagttggt
    16201 cgtttgttgc tttgataatg acgttgctca taaaattctc ctcgttttta tacataaaac
    16261 tataccgcaa ctcacgactt ttcgttttct aagagattgc gagacatagc atttttttaa
    16321 agtttatcat ataatagtta agtatgtgaa aaatatttgt ggagaacggt gaaagaacca
    16381 acttcatggt acactaaata aaactttcat caaataggag gaaatgattc gtatggcgca
    16441 cgaaaaaaca cgctacaaag cggtgattgc caaccaaaca tatactatta tcggacggga
    16501 aaccaagcat catatggata tcgtaacgaa gctgatcaat gaacagttag cagaattgaa
    16561 acaattatca ccacaaatgg ataatgaaca agcagcaatc ttgatggctg tcaatgcgct
    16621 ttcggatcaa ttgaagaaac aagaaagaat attggagctg gaagaagaaa cagcagaatt
    16681 aaagaaaaaa atgatcaaat ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat
    16741 cgagaacgaa gctcgggaag tattgaaaga aaatggtcaa gcagatcata ccattaaaaa
    16801 tcatgtggaa gcacaacaga ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc
    16861 ttctaattaa gatcctttta tcaaaataag aaaaatttaa aaaagttaga agagaaagga
    16921 acgtagcatg ctgagtttgc tgattttatt tttattgcta attgcgtttt tttctggagc
    16981 acgtcgcggg tttgctttgc aaatcatatt cgcgattgga tatgtattgt catttattgc
    17041 tgcccagcat ttttacaaac cattagcaag tcatttggaa ttatacattc catacccagc
    17101 tgtcacccca acatcaaagt tagcattttt tgatcaggta tttgcctttc acctagatga
    17161 agctttttat gcaggaactg cttttctgat tattttgcta attggctggc tgcttacccg
    17221 atttgtgggg gtttttgtcc atggattgac gtatgtgccg atcttgagac aggtagactg
    17281 gattgcagga ggcatcctgt cactaatcat ggcttacgtt actatatttt tgattttgca
    17341 gttacttgcc tttgttccat tagatgtggt ccaaaatcaa ttcggcgagc atagtttcgc
    17401 tcgttttatc gttgagagaa cgccatttct taccaacaag atgtatgagc tttgggtaac
    17461 gaatattttg aattaaggaa aatagtgctg gaaaatattt cgttttttgt acagagtagt
    17521 ttatttaaga aggctgcgta gcgtatgtcc ggcacagctc ttttttcata gtgaaagata
    17581 aggtgataaa atgaataacc gaatccttga aacattggaa tttgaaaaag tcaaacagat
    17641 ggtcagacaa tttgtagtga ctgcacaagg aaaagaagaa ctggcagaat tggtaccagt
    17701 gagtgagaaa caaacgatca caaactggct tcaagaaact gaggatggat tgaaagtgca
    17761 acgtctgcga ggcgggatcc ctatacctaa aattgaaaat atccgtcccc atatgaaacg
    17821 gattgagatc ggagcagact tgaatggagt ggagcttgca caagtttcca gagtattgtc
    17881 tacgacaagc gaattgaatc gtttcattga tgatctttct gacagtgaaa tcgaatttgc
    17941 acgtttgtat atgtgggcag atcagttagt cacgatccct gttttgagcc gccgtttaaa
    18001 ggaagcaatc gatgaagacg gacgcgtgac tgatgacgct tctcctgagt taaagagcat
    18061 aagacagaac attcggcgaa gtgaacaagc tgttcgtgaa cagttagatg ggatcgtccg
    18121 tgggaaaaat gcaaagtatt taagtgatgc gatcatcacc atgcgaaatg accgctacgt
    18181 tatcccagtc aaacaagaat accgcggtgt atttggcggt gtagtccacg atcaaagtgc
    18241 ttcaggtcag actttattta ttgaaccaaa acaagtggtg gatttgaata atcgtttgcg
    18301 tcaataccaa atcgctgaac gaaatgagat tcaacgtatt ttaagtgaac tttctgctga
    18361 acttgttccc catcgtcaag aaatcatcca caatacttat gtgattggta aaatggatct
    18421 gatgaatgca aaagcacgat ttggtaaaga agtaaaagcc atcgtgccgg gaatcagtga
    18481 agacaatcat gtcgtcttga aacaagcaag acatccactg atcgatcagg aaaaagtagt
    18541 ttctaatgac atcacaatcg gaaaagacta tcaagccatc gtcattacag gacctaacac
    18601 aggtgggaaa acgatcacgt tgaagacttt aggtctgtta caactgatgg ggcaagcagg
    18661 attaccgatt cctgcaggtg aagaaagtca aatcggtatt tttgaggaag tatttgcgga
    18721 tataggagac gaacagtcga ttgagcaaag tctatcgacc ttttcttctc atatgacgaa
    18781 cactgtagat attttatcaa aagtcaatga aaaaagtctg gtattatttg acgagctcgg
    18841 tgcaggaaca gatccgcaag aaggtgctgc tctagcaatc gcaatcttag atgatcttgg
    18901 taaaaagtct gcttatgtga tggcaacaac gcattatcct gaattgaaag tttatggata
    18961 taatcgggca aatacgatta atgccagcat ggagtttgat gtggatacat taagtccaac
    19021 gtatcgtttg ctgattggcg ttcctggacg aagcaatgcg tttgaaatct ctagccgatt
    19081 aggtcttgat acagaagtta ttgatgaagc gaagcagttg atgaatgatg agagccaaga
    19141 tttaaacgaa atgatcacag atttagagaa tcgccggaaa atggcagaga ccgaatatct
    19201 ggaaatgcgt cattttgttt ctgaggcaca agaacttcac gatgatttga aagaagctta
    19261 cagctatttc tttgaagaac gtgaaaaaga aatggaaaaa gcaaagaaaa aagcaaatga
    19321 agtcgtgtca gaggcggaag aaaaagcaga aaaaatcatt gctgatattc gtaaaatgca
    19381 acagcagatt gggcaaggaa atgtgaaaga gcatcagttg atcgatgcaa agacccaatt
    19441 agcaaatcta catcaagaag aaactttgaa gaaaaataaa gtattgaaga aggcgaaaga
    19501 acaaaaaacg ttgaaaccgg gagacgaagt gttggttacg acttacggcc aaagagggac
    19561 gcttttacgt aaaaacggga atcagtggca agtagaaatc ggtatattaa aaatgaatgt
    19621 gtccgaagac gagctgacgc ctgttgctcc tcaaaaagag ccgacacaac gagttatcca
    19681 tgctgtacga tctgaatcta gtagtcacgt gccgaatcaa ttggaccttc gaggaaaacg
    19741 atacgaagaa gcgctaagtg aggtagatca atatttggat tctgccattc tagctggtta
    19801 tcctcaagtg actattgtcc acggaaaagg aacaggcgca cttcgcaagg gaattactga
    19861 ttatcttaaa aaccatcgga gcgtcaaaag ctttgagttt gcaccggcaa accaaggcgg
    19921 aaatggtgcg acaattgtta aatttaagta aagcttacta aaggtaagca gatggttcgc
    19981 tatccaagta tagaaatgct atactagtca tagattttaa aaatggaggt ctttgatatg
    20041 tcacaagtaa ttacagataa gacatttaat gaagaaacag ataaaggact tgtattgatt
    20101 gatttttggg caacttggtg tggtccatgt cgtatgcaag caccgatttt ggatcagttg
    20161 gaacaagaat atgatgaaga agaattcaga attgctaaaa tggatgtgga tgaaaatcca
    20221 gaaactcctc aacaattcgg tatcatgagc attccaacat taatgttgaa aaaagatggt
    20281 caagtagtag aaaaagctgt tggtgttcat tcaaaagaac aactacgtca aatgatcgat
    20341 caatatttat aaaaacttac agcaaaaata agctgagggt gggactgaat ccagttccgt
    20401 cctcggtttt tttgcggttg agttaaggtt attcaaatta atattttttg agtataatta
    20461 cgttgtcagg ctggattgtt tttttgacag acatgctata ataactttaa ttgaatatga
    20521 aggggagtac cagatgaacg aacgaatcaa aaacaaatta gctttacttc ctgatcagcc
    20581 cggctgttac ttgatgaaag ataaaaatgg aacgatcatt tatgtcggca aagccaaaat
    20641 attaaaaaat cgtgtccgct catattttac tggtagccat aatacgaaaa cggaacggct
    20701 tgttagtgaa atcgaagact ttgaatacat cgttacagaa tcgaatatcg aagcattgct
    20761 cttagagatc aatctgataa agaaaaatga tcctaaatac aacattatgt taaaggatga
    20821 taaaacctat cctttcctta agataacgaa tgaaaaatat ccccgtttgg tgattactcg
    20881 taaggtacta aaagacaaag cactgtattt tggaccatat cccgatgtcg gtgcggcgaa
    20941 tgagacaaag aagatcctcg atcgattatt tcctctgaga aaatgcaaac ctagtcagac
    21001 gaaggagcct tgtctttact atcatttggg acaatgcctg tgtccttatt atttcgacgt
    21061 agatccgaaa gtctatacga aaatcgttga tgaagtgaaa aactttttaa acggaggaca
    21121 cgaaaaaatc gagtcagaga tccatcagaa aatggaaaaa gctgctgaaa acatggagtt
    21181 tgaaaaagct gcagaatacc gagaccagat ccgtgccatt gagacaatca tgactcgcca
    21241 aaaaatgaca aatactgatt tactggatcg agatgtattt ggttatgcga ttgataaagg
    21301 atggatgtgt gtccaagtgt ttttcgtaag acaaggaaag ctaattgaac gagatgtttc
    21361 aatgtttcct ttctataacg aagcagaaga agatttcttg acttacctcg gtcagtttta
    21421 tcaggaaaat gagcatttca ttcctaaaga agtcttgatt cctgatacga tcgacaagcc
    21481 cagtgtagaa gctttgttag ctacgaaagt gctgcaacca aaacgaggag aaaagaaaaa
    21541 attagttgaa ttggcgagta agaatgcctc agttgctttg aatgaacggt ttgatctgat
    21601 tgccagaaaa caggaacgga cgatcggagc agttgaacgt ttaggaaacg cgatgaatat
    21661 cccgacccca atacgtatcg agtcttttga taactctaat atcatgggga cgaatcctgt
    21721 ggctgcgatg gtcgtattca ttgatggaaa accggcaaga aaagaatatc ggaaatataa
    21781 aataaaaaca gtcaacggac cggatgatta tgcatcaatg agagaagtca tctatcgcag
    21841 atattcgcga gtgattcgcg aagggttgcc tttacctgat ttgatcttga tcgatggagg
    21901 aaaaggacaa gtagatgtgg caaaagatgt cttagaaaat caattaggaa tcgatattcc
    21961 tattgctggc atggtaaaaa acgataaaca taaaacaaat gagttgctat tcggtacaga
    22021 tttggctgta gtaccgcttg agcgaaattc accagagttt ttcttactcc agcgaatcca
    22081 agatgaagtc catcgattcg caatcacttt ccatcggcag ctacgcagta aaaacagttt
    22141 tgcttcccga ttagatgaaa ttgaagggtt aggaccaaaa cgaaaaaaaa tgctgcttaa
    22201 agagttcaaa tcgttgaaaa atatcacagc agccacgaca gaagaattac aagaaatcgg
    22261 actgccgaaa aatgtcgcac aaaacgtatt tgataaatta catcaggaat gaggaaaggg
    22321 cactttacaa agaaagtaaa gtgtcctatc attttatgta caacaaaaag agcgtttacg
    22381 ttttgttggt aaataataaa tcgcttacgt ctggtgtcta aggatatatg tgatttcttg
    22441 aagaaggagt gaagaaatgt tagaagtcaa ggatcttgtt aaaacttttg ggaactacac
    22501 agctgtcgat catgtctctt tccaaatccc cgatggaaaa atcatggggc taattggcca
    22561 aaacggggct gggaagacaa cgacttttcg tttgatcttg gattttttga cacaagacca
    22621 aggagaagtg ttatggaatg gccatcatct tggggagaaa gattatgatg tcatcggtta
    22681 tcttccggag gaacggggac tttatccgaa agtaacgatt caagaccaac tgctttattt
    22741 tgcagaactt agagggaaat caagaaaaga gattgaacca aaaatcgact tttggatgga
    22801 aaaatttcag gtaaaaggga aaaagacgga taaagtaaaa tctttgtcta aaggaaatca
    22861 gcaaaaagtc cagctgatcg ccacgttaat ccatgaacct aaattgatca ttttggatga
    22921 gccattcagc gggttagatc cggtaaatgc tgaattgcta aaagatggaa tattggaatt
    22981 gaaaaaacat ggttcatgtg tcattttttc tagccacaat atggataatg tagaaaaaat
    23041 ctgcgatcat ttgatcatgt tgcgaaacgg aaaaatggtt ttgaatggaa aagtacatga
    23101 gattcgtgaa tcatttggcc gtacgaagtt attcttggaa tctggtttaa gccaacagga
    23161 agtagaagaa atcgaaggcg tgaaaaaagt gatccttcat gaagacaata cgttagagat
    23221 taccttagaa gatcctgaag ctgggaaaga aatctttact cgtgcgactc agttcggcta
    23281 cattcctatg tttaaccagc aaccgccaac tttggaagag attttcaaat tgaaagcagg
    23341 tgaaatcgat gcgtaagttt tggattatta ccaaagatgt ctatttaaaa aatgttaagt
    23401 cgatttcttt cttgatcatg atattagtcc cttttatcat gctgggtatc atttatgtag
    23461 caggaaactt tgctcaacaa aatagtgatg tcaataaaat cggcgtattg gctgataatg
    23521 agcaaatcag tcagcaaatg agccaagtca aaacagatga ttttgagttc aaagcagttg
    23581 attctgaaga aaaagcacaa aaagagcttt cggaagaaaa aatcgatgcg tatttagtag
    23641 tctcaactga gaatgataag ataaatggga tgctttatag cgaaaattca ttaggacaaa
    23701 gtacacaact actgatacaa caacaactga ctggcttgca atcgatgctt cgagcgagtc
    23761 aattaggcat ttcaccagaa gaagtagcta gcttgagcca acaagcaagt ttgactcgac
    23821 aaaaagtcaa ttttgattca aaaggaaaaa tgacagtcgg agaagataac tcgacagtcc
    23881 aatacgtcgt aagttatgta gcaacgattg tcttgttcat tattattttg acgtatgcgc
    23941 agatcattgc acaagagatc gcttctgaaa aaggcacgcg gatcatggaa gtgatcctgt
    24001 ccagtacgac tgcacaaaag catttttacg gaaaattaac aggtgtttta cttgttgcat
    24061 taacgcaaat ggcattatac ggtattattt ttggtatagg atacagtcaa ttcaaagata
    24121 tggaaatggt caaaaatata ttagacggta tctcattgga taacttgatt ggaccattct
    24181 tgtggtattc tcttctattc atgatctttg gcattttgat tttttctgtt ttagcagctt
    24241 tatgtggctc tttggtcaac aaagcagaag atacagcaaa agcaattttg ccagtgactt
    24301 atctatctct aggcggatac atgcttggat tgatactagg agcttcagat ccaaacaata
    24361 tcgttattcg gatcacttcg tatattccat tcttatcttc atatattatg ccgattcgct
    24421 tagcaaatga gacagtcggt atgggtggag cattgatttc cttgttggta cttgtagccg
    24481 taacattctt attgatgatc ttgtctgcaa acatgtacaa atcgaatgta ctcgtttata
    24541 gcgaaggcgg attatggagt tcattgaaac aatcgatttc aattatgagg aatgaacgaa
    24601 aaaaagcata aaacaatcct aaaatacaga aggaagctgg acatctattg ttcccagctt
    24661 ctttttgtat agacctattt tatttatttt actatattgg atatatattc attgaaaata
    24721 gtgtataaca actttataat attgagtata ccaatttttg gaggcgtttt aatggaccgt
    24781 tatttagatt tactttcaga aaaattcccc tcaacggagg atgtcatcac ggaaatcatc
    24841 aatctcgaag caattttgca gcttccaaaa ggaacagagt tatttttgag tgatatacat
    24901 ggcgaatttc ctgcttttga tcatattctg cgtatcggtt caggaaatct taaagaaaag
    24961 atcaaagacc ttttttctga acaaatgacg gaaacggata tcagccaatt cactattttt
    25021 acggcttatc cagaatatgc attaacgact gattggtaca aagaacaaga taaatcgcag
    25081 ctgatccatc atttgatcga tcttttacgg tttacgacag tcaaatatac ccggtcaaaa
    25141 gttcgaaaag gattgccgaa agaatatagc tatatcattg aggaactctt gtatatcgat
    25201 gaccgtatca atggaaaaaa agactatgta aaaaaaatca ttgaacaact tgttttgatg
    25261 aaggaagaag aaaaattttt aacgaaactt gcacagacga tccaaaaatc tgtcatcgat
    25321 catttgcata tcgttggaga tatttttgac cgaggaactc aagcagccaa agtgatggat
    25381 cgaatcatgg atttttcttc tgtagatatc caatggggaa atcatgatat tttgtggatt
    25441 ggtgcgtatt gcggctctga ggcatgttta ctgacacttt tacggattgc agcacgctat
    25501 aattatttat ttgaattaga gaaagaatat gggttgaatt tacgtccatt gttttctttt
    25561 gctcatcaaa cttatcagaa gaatccagta tttgcgccta aaaacagtaa aaatctatcc
    25621 gaaagagagc aagaagaatt agaaaaaatc catcaggcac tttctatcct tcaattcaaa
    25681 tcagaacatc agttattgga tcgtcgacct gaatttaaaa tggacgatcg taaattgctg
    25741 gaaaaaatcg actatgaagc atcaacgatt gatttagaag gtcagctcta tgagttgaaa
    25801 ggaacttgtt ttcaaacgat ccagccagat gatccaaaaa aattcttgga agaggaacaa
    25861 aaagtgattg atagtttgat gtattcattc cagcactctt tgagaatgca aaaacatatg
    25921 acgtttttga tcgaaaaagg gggaatgtat ctcacgaaca atcagcatgt gctgtttcat
    25981 ggctgtatac ctgtagacga gaagggacag ctcttagcat ttgaattaga ccaatcttat
    26041 cgcggaaaag aactgctggg cttttttgaa aaacagataa ctgaaagcgc aaaagatctg
    26101 actgcaaaag aagatttagc aaccgatttg atttggtatg cgtggagtgg gccattttcg
    26161 cctttattcg gcaagagtaa aatggcgact tttgaacgtt attttctcac agagcctcat
    26221 acgcacgtcg agaaaagcaa tccttattat cacttgcgag atgaagagtg gttcagtgaa
    26281 aaaattttag cagaattcga cacaaaagaa gaagggtcag caatcattaa tgggcacacg
    26341 ccagtaaaag taaaaaaagg ggagtcaccg atcaaagcaa atggaaaagt atttgtcatt
    26401 gacggaggtt tatccaaagc ttatcaaaaa acgacaggaa ttgccggtta ttcactatta
    26461 tgcaattctt atggtttcca aatcgtgaca cattatccgt ttttgcctat tgaacaacaa
    26521 tttgcaaaaa aatccgacca aacaaatgtg aaaaaagtaa tcgaacagca actaccacgc
    26581 aaactcaatc gtgatacaac aaaaggtatg gaactgcagc agcagattac ccagctcaaa
    26641 aggctgttgg actatcgaaa ggacgattga gaagaattcc agtgaacatt gttccataga
    26701 ctggagactg agacaaaagt cgtgtcgcag tctctttttc cggataaacg gtgttcttct
    26761 gccaactcct cggtcacaag tcacactatt caacaaacat gaaaagctgt tttttgaaat
    26821 tgctccttgt gactcagagt tgaacggcag gctcacgacc tcttggtata aacggtgttc
    26881 aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtgaaaag ccattttcac
    26941 aaaatttttc ttaatactcg aaactaaaca gctttttcac aaccccctca gaaagtctat
    27001 taaaattcat tcacaaagaa atcgaataag cgctgttcag atggatgtac acggtgggta
    27061 agttccggat gccattgaac accgaggatt ttatgcatat catctttgct ttgaaccgct
    27121 tcaacgagtc catcgttaga aaaagcgatg gcttttaata aaggtgaaag atctttgact
    27181 gcctgatgat ggtaagaatt cacaaaatat ggatcgggga gaaggttgct taacaaactg
    27241 tctgaaacaa tcttgacttc gtgagtcgta aattgcgggg cagtgggctg ttgttcatgt
    27301 tttactgacc actttggata taatgaaaga tcttgataaa gtgtaccttc caaaacgaca
    27361 ttcaatagtt ggagtccccg gcagactgca aaaattggtt ttctttgttt caatgcttcc
    27421 aagatcagtg cctgttcaaa ttggtctcgt tgaatatttg tttccgttag ctttggatga
    27481 ggatcttcca tgtaaagttg tggactgaca tcttgaccac ctgcaagaat aagtttgtcg
    27541 atttgtccaa tcagttgagg agctgaatct ggagacatga ctggcagaat aagaggcaat
    27601 ccttgagcat ctaaaactgc atccacgaat ccttgcggtg tatagctcac ttgatttcct
    27661 tggaaagtat ctgttgctcg aatcagttga tttccggcaa tgccgatgat tggtttcatt
    27721 tgtattcccc ctaaagtaga cttattgtaa caaggattta aaaaaaatta aactatgtaa
    27781 gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag aaatttcgtg
    27841 tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa agtaagcaga
    27901 aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga gaaggacatt
    27961 ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt caatgaaaaa
    28021 agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta gaagctttgg
    28081 aacaaatccg aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg cggcagcagc
    28141 tgaagatcta tgcaacagaa aaagagcagg aattggaaac gatccaaaca aactatcgac
    28201 aacaactaaa acagttggtg gaagaaaaag aacaatttga aatacaagaa ttagaaaaag
    28261 aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa gatcaatatc
    28321 aaaagcatca gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa acttatggcc
    28381 gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga gaggatttta
    28441 caagcagtac aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa tagaacaaac
    28501 aaccagtggg tggagaagta ttttccagat accacgatta ttgataaaga agcaaaatta
    28561 agcgctttag gtaatcggtt gaaagacatc caagaagcta tccaattcat acaacaccac
    28621 ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt acaagatttg
    28681 gaagccaatt attccgaaga aaatttcata aataaattga aagaagtcct tgatttaaaa
    28741 gaaaagtggg aacaactaac tgaagaacgt gatcgtttag aagaagagga agaatggctt
    28801 cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac gaccattttt
    28861 gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact gagtaagctt
    28921 gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct ttcgtatgtt
    28981 gctttaaaaa aatcagttgc tgttatagaa gaaattgctg ggaaatacgg gttcaataaa
    29041 gaaacgtatc cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa gcagcaatta
    29101 tccattgttc acgagcagca aaagaagata acgacagcgt taggtcagtg cagtggctat
    29161 atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg agaaaaaata
    29221 aaagaacggt ttatccatgc gacttatctg atcgttttgc aaggttgggt agatgcagaa
    29281 gaaaaacaga atttgctgca tgtgttaaac gaaaccgtat cagaagaaga tatatacgtc
    29341 agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa attgaaaaac
    29401 catccgcttg tcgaaccttt tgaattgctg actgaaatgt acagtctgcc aaaatatgaa
    29461 gaagtagatc caacaccttg gatgacacca ttttatcttg tcttcttcgg gatgatggtc
    29521 gcagatgttg gttatggttt gctgatgctc attgggacaa tactagctca aaaactactt
    29581 gttctaccac gcgggatgaa acgtttcgta aaattctttg agattctctc gataccatcg
    29641 atcatctggg gattgatcta cagttcattt ttcggacagt cattaccaaa agaactgttt
    29701 ggcatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac gattttgatt
    29761 ttatccgtga ttttcggtct gatacagatt ttggttggat tatttgtggc agcaaaggaa
    29821 aatatcagga gaaaagatta cttgagtgct gtcagtgatg gatttgcctg gcaagggatc
    29881 ctaattggga tcgttattgc gttggttggt gccatgttga taaaaaatac agcatttgtt
    29941 taccttggcg ctgggctatc gatcattgct gccttatgta tcttgatcgt tccgattatc
    30001 caaacgccgt ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta cggactgacc
    30061 ggctatatcg gtgatttagt cagttataca cgtttgatgg cattgggtat ttcaggcgga
    30121 agtattggag cggcattcaa tatgctagtt gctttcatgc cgccagctgc tcgattcagt
    30181 gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac actgctaagt
    30241 gcctatgtcc atggcgcacg attgcagtat gtcgaatttt ttgggaagtt ttatacagga
    30301 ggcggacgtg cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat caatcacaag
    30361 aaaaaaaaca ataaataatt ggaggatttt tatataatga tggattactt aattagtcaa
    30421 aatggtggaa tggtatttgc ggtattagcg atggcaacag cgacgatttt ttcagggatc
    30481 ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcattgac aacaagtcaa
    30541 cccgaaaaat tcgggcaggc tctgattctg caattgcttc caggcacgca aggtttgtat
    30601 ggattcgtta ttgccttttt gatttttatc aacttaggaa atgacatgtc agttgtacaa
    30661 ggactgaact ttctaggtgc ttctctgccg attgctttta caggcttgtt ttcaggtatt
    30721 gcccaaggaa aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc tgaacatgct
    30781 acaaaaggaa tcatttttgc tgcgatggtt gaaacatacg cgattttagg tttcgttatt
    30841 tccttcctat tagtgttgaa tgcctaagca taaaggagga aaagcagatg aacgccattg
    30901 aaaaaatcat ctctcagatg aacgaagcag cagagcagga aagagcggcg ttagaacaag
    30961 aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaagtt gaaacagaac
    31021 atcaaaaaca aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt cagctaagga
    31081 atcgccagca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa tttttaagac
    31141 gattgtttgc agatgcggta actgaaatgg aaaattggga tgaatctgaa caaatccagt
    31201 ttatcaaaaa tgcattgtat tcattacctt tgacaggaaa agtagcattc attgctggag
    31261 aaaaatcagc agcctatctt tctcagacgt tgttggatga atggaacaat gaacttccct
    31321 ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg ataaacgatc
    31381 aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa ggaacgatga
    31441 gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg aataccacga
    31501 actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga catttgaacg
    31561 gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca cgatttacag
    31621 tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa aggagcagag
    31681 ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt ggatctatac
    31741 gatgcgttat actttccata atctgaaggt tttgacaaaa gctgagatga caaatcaaaa
    31801 tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga aggatgccat
    31861 tcatacgcaa gtttcaaatg aacttcctga aagcattatg gacagtatac gagaagtacg
    31921 tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg atcgacagtt
    31981 tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc tgacagaagt
    32041 cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat tgcagaaaag
    32101 aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa aagaagtatt
    32161 tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag agacagatta
    32221 tgctgatttt ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa agcttgaaca
    32281 gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg ctttcggacc
    32341 actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc ttcgtttact
    32401 ggttatcggc aaaagaagcc atttcgaatc agaaacaatc agaaagcggg tgagacaagt
    32461 ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca ttcaaattgt
    32521 ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca attgaagaaa
    32581 tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca ttgagcccag
    32641 aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt cccaatcatc
    32701 aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa gcagtaggtc
    32761 aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa atcataaaag
    32821 tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa gacatgtgtc
    32881 tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat gtggcgtcta
    32941 ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc tctactggtg
    33001 aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac ggaatccaac
    33061 ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc ggagttcaat
    33121 tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa gccggagaaa
    33181 cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata aaacataaaa
    33241 tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt tcattcacta
    33301 ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg acgatgcttc
    33361 aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg gatgcaccca
    33421 tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga ggagcagcag
    33481 ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt gcaaaatggg
    33541 ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag atgacggatg
    33601 ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg atggaacgta
    33661 cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct tctatctata
    33721 cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct atcatggcag
    33781 actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg gaagaaatgc
    33841 ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac tatgaacgat
    33901 ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc gcaatcagtg
    33961 ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca ttgcgggtcg
    34021 taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt ccttcgatcg
    34081 attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg gatcatatcc
    34141 ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag gaagaacagc
    34201 agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat gaccgtctga
    34261 cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct tttgatgatg
    34321 tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc ttgacctttg
    34381 gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc atggacggta
    34441 ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca gaattagaaa
    34501 aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt tcagaaggag
    34561 gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc attgatggcg
    34621 gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat gcaaaatggc
    34681 gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt ccaaatattc
    34741 gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg ccatccttta
    34801 gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg acgaccgaaa
    34861 gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga agtaatcaat
    34921 ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc aatcgatcat
    34981 ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg tcttcctcat
    35041 aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc agatgatttc
    35101 gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt catggaagat
    35161 tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc aaacgaccct
    35221 gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata tcttgcttat
    35281 gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc agaggcattg
    35341 cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc tgggtatctt
    35401 tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact aaaaggatcg
    35461 gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc aatccctgac
    35521 ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta caagagtggg
    35581 attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa gggaactgga
    35641 cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc agcctatgca
    35701 caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt atctgatgta
    35761 gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa tcaaggattc
    35821 tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct atcgatgttg
    35881 ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct gccagaaggg
    35941 aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt gactcgattg
    36001 aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa gcaagatgaa
    36061 ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca agaaatggaa
    36121 gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat cacggaacct
    36181 ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat cgtagacaaa
    36241 aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca gctgacagac
    36301 acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc aatcgatcgg
    36361 tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac ttgtcaactg
    36421 atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata tatgacgatc
    36481 cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa cgagcgcgca
    36541 gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt ggtatctttt
    36601 tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt ttgccggctg
    36661 ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc ctacttaccc
    36721 taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt ttcactgcaa
    36781 cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat tggaacagtg
    36841 ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt atgatggttc
    36901 cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt attatattaa
    36961 aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt tacatcctaa
    37021 aattggcctt tgtgatccaa ttgattggta cggttgcttt atcttttgta tttgtgcctg
    37081 aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct agtttttgta
    37141 atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat gtttatttga
    37201 tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta tggcgtgatt
    37261 tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca ttaaccgtta
    37321 caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc aatggggcaa
    37381 cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg agtgtgacac
    37441 ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca ggtctgattt
    37501 taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc ggattgaaaa
    37561 caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga cgtacgagag
    37621 cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt actttatttt
    37681 ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact gaaacgatac
    37741 ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt ggtacagtag
    37801 gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc attatagcgc
    37861 ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg acaaaagcac
    37921 aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga taatttcatc
    37981 ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt tcttgcttct
    38041 tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt tgttgaacct
    38101 cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt tatttatcaa
    38161 taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca gaagctaaaa
    38221 tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc gcacgtgtag
    38281 taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc tcaattatct
    38341 tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga ttcgttggtt
    38401 tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga cttaatttag
    38461 tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact tccacatcta
    38521 atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt tggacatgta
    38581 ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc attccagcta
    38641 agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta tgttgatagt
    38701 aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct ttttcaatca
    38761 cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga ttattacaag
    38821 aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc aatatatcag
    38881 gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat aactgagcca
    38941 attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct ggagagaaaa
    39001 gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat agagattcta
    39061 aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat tgaaaatttg
    39121 tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta ctagttgatt
    39181 gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga ccaaaatcaa
    39241 tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca ttgggagaaa
    39301 taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca tatttataag
    39361 ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt tgggagggta
    39421 actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt tgaatcaacg
    39481 atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac gggtaattgt
    39541 cataagaatt aataggcatt ttctccactc cttaaaattg tataccatac aattttacca
    39601 ttgattgtat tgattttaga agttaatctt ttgtgaggag ggaattgtct atgaaaatta
    39661 ttaattttga ggaagtagta gcttctttta gttttgaaga agcgattcaa gtaatgaaaa
    39721 aatgtttttt tgattatgaa agtggtaaaa tcagccaaaa tcctcgattg gtggaaattt
    39781 tacctgacgg aaaaggcaaa aatatgtttg cgatgatgcc tgcttatttg ggggagaaca
    39841 gatattttgg cacaaaagtc attaccgctt ttccggataa tcatcagtat catttacctt
    39901 ctcatttagg agagattttc ttgtttgatg ctcaaaacgg actacccaaa gcattgataa
    39961 acgcaaatgc agtcacctgg attcgaacag ctgctgtatc tgcgctggca acagattact
    40021 tagcaaatcc gcatgcagaa acacttgctt taattggagc tggtcaacaa gcattgtcgc
    40081 atttgactgc gatttctacc attcgtccca tcactacagt gtttgtttac gatattttag
    40141 aagaaagacg aaatactttt atcgctaaag ctaaaaaaca gtatcctaaa ataacgttta
    40201 taaatgcaat atcagtagaa gatgcagtac gtccagcgga aatcatttgt acgttgacct
    40261 ctagtaaaga ggcatttctt caaaaagagt gggtaaggga agacgcacat atcaatgctg
    40321 tgggcacatt tactcctgat acaagagaga taagtacatc gttgatggaa acttctcaaa
    40381 tctatgtaga tgattactca gctgccttaa cagaaagcgg ggacctgctt atagccatgg
    40441 cagaaggaga tttagaggaa tcttgtattg ttggtacatt aggcgaatta gtttctgaga
    40501 aaaaaacatt ttataaagga gaatcgaggc cgacgatttt cgacgcagtt ggtctagcta
    40561 ttgaagatct ttgttgtgca gaatatattt ataacaaatt agagaaaggg gctttaaaat
    40621 gaacttagat tattacgtag ttgatgcatt tgcagatgag gtgttcaaag gaaatccagc
    40681 agcggtttat gtgctagaag aatggctgcc agaaggtacg atgcaaaaaa ttgccattga
    40741 aaacaattta tctgaaacag catttacagt gaagaaaaac caagaatttg agcttagatg
    40801 gtttacgcca gatcgagaaa tcgatctttg tggtcacgcg acattagcaa cagcatttgt
    40861 tttatttaac tattacaaaa tacctgatga aacgattaaa ttttctactc aaagcggtaa
    40921 tctatttgtg acaagaatta aagattatta ttacatggat tttcctagta ttatgccgaa
    40981 aaaagtgccg atattgtctg aatatgaaga agcgattggg gctaagatta aagaagccta
    41041 cttagcacgt gatttatttt tcgtattaga agatgaaaaa acggtggcta aattgcagcc
    41101 agattttaca gctatcaaaa attttgaatt aggaatagga gtcatcgtga cagcagaaag
    41161 tgtgcaaaaa gactttgttt ctagaacctt ctttccaaaa ttaacaatca atgaagatcc
    41221 agtatgtgga tcagcacatt ctaatcttat tccttattgg gcagaaagaa tgaataaaac
    41281 aaaattgtca gcttatcaaa tgtcatcaag gggcggggat ctagaatgtg aattaaaaga
    41341 agatcgtgtg ataataggag gaaaagcagt tttgttttct aaaggtacct cgtttatttg
    41401 atttctttca aatgaattaa aaataaattg gtggagagtg gcaaccttat agctacaatg
    41461 gttgttaaaa aaagacgcaa actttttcaa tttaatgaaa agtttgcgtc tcattttaat
    41521 tattggtgtt tgttttttat tcaatcaaga gtaaaatgcc ccatactaac tatcctcgga
    41581 tgacttcgat tttataacca tcagggtcaa tgacaaagta gtaagaaggc gcagtgccag
    41641 gtaatccttt taagtcagta atctcaagac cagcagcttt atgttgctcg tgtaattttt
    41701 ctagatcatc agctgctata gcgatatgac catagccgtc tcctagatca tacgcaccat
    41761 gatcatagtt gtaagtcaac tctaattcat agtcatcccc aggaagtgtc aaataaacaa
    41821 gtgtaaattt gttttctgga aaatcacggc ggcggctttc ttcaaaacca aatgcctttt
    41881 gataaaattc gattgatgct tcaagatctt tcactcgaac acatgtgtga gccattttca
    41941 tagattcatc tctccttttc cttttactca atcataacaa aaagaagtaa ggaaatagtt
    42001 gatttgtgct tgttcatgag aaatttctca tgtaaaatgg atagaaaata aaactgttaa
    42061 ggtggaaact attcatggat aaaccaattt ttggtgaaaa aaagcaattt gctgattatc
    42121 aaacccgtta tgcagcatat attgtcatag aaagagatca gcagattgca gtaatcgagg
    42181 ctccaaatgg tgcttttttt cttcctggcg gggaaattga aggagaagag acgaaagaag
    42241 aagcgattgt ccgtgagctg atagaagaaa tgggaattgt ggcagaaatt tcttcttatc
    42301 tgggagaagc tgatgagtat ttttactcga attatcgtgc aacttattat tataatccag
    42361 gatatttctt tgttgctgat tcttggaagc gtataggaga accaactgaa aagaccaata
    42421 aaatttggtg ggtgagccct caagaagcgc ttgtgaaatt gaaacgtggt agccaccgat
    42481 gggcagtcga gagatggatc gaaaaaaata aaaactgaaa gaaagcgagt agatcagctt
    42541 tctctcagtt tttttattta ttacagtaca tattttttga ttgcttcagc tacaccgtgc
    42601 tcgtcgtttg atgaagtgat gacatttgct gcttctttga caagagaaac ggcatttccc
    42661 attgctactc ctagaccagc atattcaatc atggataagt cgttctcgtt atctccgata
    42721 gccatgattt catcttgatc aataccaagg tgctgtgcga gattcgcaac tgccgcacct
    42781 ttacttgctt ctttatttag tacttcgaag taatagggcg cacttttgac cgttgtgtat
    42841 ttatcacgga aagtttgcgg taaacgcgca attgctgcat ctaaaatttc tggttcgtcg
    42901 atcatcatca ttttgacgat gttcatgtct ggtgtcattt cttcaggtgt tcgatatttt
    42961 agcggcatct tgactaaact tgcttcgtgg atcgtataag cgctgatatc tcgattaggc
    43021 gtgtagatcg ctttgtcatc gatcgaatgg agatgactgc cgacacggcg tgccattact
    43081 tcgatctcta ggaaatcttc atgtgtcagt ccgtgacggg cgatgatttt acctgatttc
    43141 gtttgttgta cgagtgcccc gttataagta ataacgtaat cattatcttg aaaaaggttc
    43201 aactcgttta actgttcctg gacacctggt aagggacggc cagtgcataa aacgatcgaa
    43261 acaccttttg caacagcagc ggcaatcgct tcttttactt cttttgttac ttctcttttc
    43321 tcatttaata aagtaccatc gatgtcgata gcgactagtt tgatagacat aatttcctcc
    43381 aatatatttt aatttatttc aatcaatgcg ccattacgga tatggctggc aaattcttga
    43441 taagtttcgt caaataaatc ataatgatca cgcaaggacg gatcaaccat ttcttttgga
    43501 aagaaaaatc gctcatctcc ttgttcttgt cccgctaatg cagcaacaag ctgactaacc
    43561 tgtgataatt ctatcaaagt tccgtcattt cgcaaaatct cgatttgtgt gcgatgtctg
    43621 ccttgttctg ggcgataaaa atcataaggt agatcgtagc tagaattcac agcagtgtaa
    43681 taaacaggat tatatccgac cttttctact aacaaagtga gttcttggac taattttgaa
    43741 tcccggttgc cagaaaaagt agcagatttc agtggtttac gattcaaaaa acgtttagct
    43801 aaatcattta aaataggatc agcagattca gaccattgag taaagtaagt gcccaagacc
    43861 ccatcatcaa gtttgaggta ttcttctaaa gtaaagcttc cgtcaagaaa tggcagaagt
    43921 aattgagaat ggagttcaaa actgctggca tcttcttgat acaactcgtg tgcgcgatga
    43981 agcagatgct ccaaaattac ttccatacca cgggaaacag gatggaagta cacttgcaca
    44041 tacatttgat atcggctaac gatataatct tccactgcat gcatgccact catggaaaaa
    44101 gcgatcccgc cttcataagg acgaatcaca cgtaatattc gggtaagatc aaatgttcca
    44161 tattctgttc cagtgaaata agcatcccga agcaaatagt ccattcgatc tgcatcgatc
    44221 tgactagaaa tcatttggac aacttgtggg ttggggtaag ttttttggat cacactagct
    44281 accttttcag gaaaaccttc ttctactcgg ttaagaatct gatacacttc tgtttctgga
    44341 gaagtgatga tctctacagt gatggcttca tgattcgtat ggaagatatg ttcaaatgta
    44401 tgagagtaag gaccatgacc gacatcatgc aacagtgccg cacataaagt caccagccgt
    44461 tcctgatcat cccagcctcc gttgcctatt ttttcttttg aaaaatttcg agaaaagata
    44521 tcgcagattc gtcttgaaat ctcataaacg cctaatgaat gagtaaaacg gctatgttct
    44581 gctccgtgaa aagtgaaaga agaagtgcct aattgtttaa tgcgtcgtag acgttggact
    44641 tctttcgaat tgatcaaatc taagattact tgatgctgga catggatata gttatggact
    44701 ggatcacgaa aaactttttc cataggcagc atttgatatt tataaggggt tgtcataatg
    44761 tagctccttt atgttattac gttgttccat tctctggatt tggttgttcc attgctccag
    44821 ctggatttct ttttttagcc agtcggtaga atcctgtaaa tcacttgcag aatacagctt
    44881 ttcaaaagct tttgcaaatg cttctttgac ttcttttaca aaaaggggtt ggtcaagcag
    44941 ttcttcaagt gttgccattg tagcaggatc cactgcagga tagccatttg taccaaagtc
    45001 ttcttttaaa gcagattgat aaaatcgctt catgacttgc ccacgttttt cctgattacc
    45061 gttgacactt aagtacatca taacagaaat cccatttttg atccgacgct gagcgatacc
    45121 ggcaaatttt ttgccatgga tgcttaaatc ataagttcca ggacaataag aatgggtgat
    45181 ttcgaatgct tgtatcggtg cctttggaaa agcgtattta gtcaatagga gcatttgctc
    45241 gtaagcgtca tctatggtta ttttttttgt gtcactttgg gggaaaatca aagaaatatt
    45301 caaaattcct tcatctgata cgatccctaa tccacctgaa tttctgatca ctggagcgta
    45361 acctgcatct tccagtgttt ggatcccgtc acttagaaaa ggagttcgaa catccttcat
    45421 acctaagatg actgtgcagt catattgcca gaaatgtaaa aagatttgct ggtgttcttt
    45481 agaatattcc gttaatatat ctgttaatgc aaaaggggaa aagtcgtttt tttgataaat
    45541 gtgctgatct agtcgaatcg cagggttcat tcaaccggct cctttgtata aattcatatt
    45601 ttctacaata ttatactcta aaatcaaaga gttgttttgg aaaaaaggtt ttctgtaaaa
    45661 atggacaagt tggttgacaa aacaaaagaa actaggaaca atgtagctat gagaagtaaa
    45721 ggagaatgaa taatggaatt atcacaagga acaccgattt caatcaaatt gcgtacaaaa
    45781 gtaaaacagc aaggggaagt gcaagatttt tattttgatt tgaaagggca aatggtgaca
    45841 attggcgata cgctgtacat acgttataaa gagattcagg aggagacggg cgatgagatt
    45901 cctgtaacga tcaaattagt tccagatggt catgtacagc tgatccgtgc aggagaaatg
    45961 cggatgcgtt tgaaatttgg ctataaagaa cggttagaaa catcctatcg aacaccatac
    46021 ggtatgatcc agattgctac ttttacgaaa gaactgcatg ttagtttgaa agaccgtccc
    46081 acagcaggga aggttcgaat cgattatgat ctgttcatgg gaccggaaaa aatcggcgaa
    46141 tattatttga ccttggattt tacagcgtaa atataaaaga aaagtttgat tttttgtgtc
    46201 tgattttgta tgataaggag tagactgtga aaggacgtgt cctgattgga aattagtgta
    46261 tttgaaggtg caaacaaaag cgaattatca atgatcgaag tagcacacgc gatcttagaa
    46321 caacgtggcg atgtcatgga tttttctgat ttagctaacc agatccaaaa ctatcttggg
    46381 aaatcagaca gtgaaattcg tgattcactc gcacaatttt atactgactt gaatattgac
    46441 ggcagcttta tctcattagg ggataatcgt tggggcttgc gttcatggta tgctatcgat
    46501 tcgatcgatg aagaagtaaa ccatggaatg gacgaagagg atgaagatac tccacgtcgc
    46561 cgtaaacgca agaaagtcaa cgcctttatc aatgatgatg aagacgcgat cgattacaat
    46621 gacgatgacc cagaagatac agatctgaca gaagaagatg acgatgatct gtttgatgac
    46681 gatgatgatg aagatgaaga aatcgctgct tataattctg acttgcaaga aatcggagca
    46741 gatgacccga cagatgatga agaagacttg ccaggaaata tcgaagaaga tttaagtatc
    46801 atagacgaag acgatgatga tgaagattat gaagaggaag aattttctga ctctgaaaaa
    46861 gaataaacat tattttctta cgagaggttg tgacagagac gatcagccta tcagaataag
    46921 gaggtgtgac acgatttt
//
LOCUS       DAGCWU010000004        44954 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.004, whole genome shotgun sequence.
ACCESSION   DAGCWU010000004 DAGCWU010000000
VERSION     DAGCWU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.004"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            212..604
                     /locus_tag="MBZ71_000183"
     CDS             212..604
                     /locus_tag="MBZ71_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4428166.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="MBZ71_000184"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="MBZ71_000184"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBT4428167.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="MBZ71_000185"
     CDS             3165..4775
                     /locus_tag="MBZ71_000185"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBT4428168.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="MBZ71_000186"
     CDS             5067..5936
                     /locus_tag="MBZ71_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBT4428169.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="MBZ71_000187"
     CDS             6101..7372
                     /locus_tag="MBZ71_000187"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBT4428170.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="MBZ71_000188"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="MBZ71_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBT4428171.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="MBZ71_000189"
     CDS             8763..9026
                     /locus_tag="MBZ71_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBT4428172.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="MBZ71_000190"
     CDS             9471..9920
                     /locus_tag="MBZ71_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4428173.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="MBZ71_000191"
     CDS             9977..11395
                     /locus_tag="MBZ71_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4428174.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="MBZ71_000192"
     CDS             11395..12255
                     /locus_tag="MBZ71_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBT4428175.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="MBZ71_000193"
     CDS             12429..13202
                     /locus_tag="MBZ71_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4428176.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="MBZ71_000194"
     CDS             13349..13714
                     /locus_tag="MBZ71_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBT4428177.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="MBZ71_000195"
     CDS             complement(13862..15070)
                     /locus_tag="MBZ71_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4428178.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="MBZ71_000196"
     CDS             complement(15089..15367)
                     /locus_tag="MBZ71_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428179.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="MBZ71_000197"
     CDS             15521..16216
                     /locus_tag="MBZ71_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBT4428180.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="MBZ71_000198"
     CDS             complement(16658..17455)
                     /locus_tag="MBZ71_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBT4428181.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="MBZ71_000199"
     CDS             complement(17452..18636)
                     /locus_tag="MBZ71_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBT4428182.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="MBZ71_000200"
     CDS             complement(18661..19908)
                     /locus_tag="MBZ71_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBT4428183.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="MBZ71_000201"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="MBZ71_000201"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBT4428184.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="MBZ71_000202"
     CDS             21888..22202
                     /locus_tag="MBZ71_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBT4428185.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="MBZ71_000203"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="MBZ71_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBT4428186.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="MBZ71_000204"
     CDS             22887..23531
                     /locus_tag="MBZ71_000204"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBT4428187.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="MBZ71_000205"
     CDS             23546..23875
                     /locus_tag="MBZ71_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBT4428188.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="MBZ71_000206"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="MBZ71_000206"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBT4428189.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="MBZ71_000207"
     CDS             24863..25687
                     /locus_tag="MBZ71_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBT4428190.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="MBZ71_000208"
     CDS             25680..26027
                     /locus_tag="MBZ71_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBT4428191.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="MBZ71_000209"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="MBZ71_000209"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBT4428192.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="MBZ71_000210"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="MBZ71_000210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBT4428193.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="MBZ71_000211"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="MBZ71_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBT4428194.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="MBZ71_000212"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="MBZ71_000212"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBT4428195.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="MBZ71_000213"
     CDS             31657..32451
                     /locus_tag="MBZ71_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428196.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="MBZ71_000214"
     CDS             32441..34483
                     /locus_tag="MBZ71_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428197.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="MBZ71_000215"
     CDS             34764..34913
                     /locus_tag="MBZ71_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBT4428198.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="MBZ71_000216"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="MBZ71_000216"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBT4428199.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="MBZ71_000217"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="MBZ71_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBT4428200.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="MBZ71_000218"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="MBZ71_000218"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBT4428201.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="MBZ71_000219"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="MBZ71_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBT4428202.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="MBZ71_000220"
     CDS             39330..40412
                     /locus_tag="MBZ71_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBT4428203.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="MBZ71_000221"
     CDS             40412..41215
                     /locus_tag="MBZ71_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBT4428204.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="MBZ71_000222"
     CDS             41285..42025
                     /locus_tag="MBZ71_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBT4428205.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="MBZ71_000223"
     CDS             42036..43040
                     /locus_tag="MBZ71_000223"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBT4428206.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="MBZ71_000224"
     CDS             complement(43085..44929)
                     /locus_tag="MBZ71_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428207.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14459 a         7837 c         9348 g        13310 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgattt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt agtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcattcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgt
//
LOCUS       DAGCWU010000005        42995 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.005, whole genome shotgun sequence.
ACCESSION   DAGCWU010000005 DAGCWU010000000
VERSION     DAGCWU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.005"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            138..1130
                     /locus_tag="MBZ71_000225"
     CDS             138..1130
                     /locus_tag="MBZ71_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4428208.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1190..2611
                     /locus_tag="MBZ71_000226"
     CDS             1190..2611
                     /locus_tag="MBZ71_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBT4428209.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2604..4103
                     /locus_tag="MBZ71_000227"
     CDS             2604..4103
                     /locus_tag="MBZ71_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBT4428210.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4142..5536
                     /gene="uxaC"
                     /locus_tag="MBZ71_000228"
     CDS             4142..5536
                     /gene="uxaC"
                     /locus_tag="MBZ71_000228"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBT4428211.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            5695..6378
                     /locus_tag="MBZ71_000229"
     CDS             5695..6378
                     /locus_tag="MBZ71_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBT4428212.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            6380..7216
                     /locus_tag="MBZ71_000230"
     CDS             6380..7216
                     /locus_tag="MBZ71_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBT4428213.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            7227..8981
                     /locus_tag="MBZ71_000231"
     CDS             7227..8981
                     /locus_tag="MBZ71_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428214.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(9257..9598)
                     /locus_tag="MBZ71_000232"
     CDS             complement(9257..9598)
                     /locus_tag="MBZ71_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBT4428215.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(9666..11837)
                     /locus_tag="MBZ71_000233"
     CDS             complement(9666..11837)
                     /locus_tag="MBZ71_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBT4428216.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            12006..12905
                     /locus_tag="MBZ71_000234"
     CDS             12006..12905
                     /locus_tag="MBZ71_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBT4428217.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(12976..13833)
                     /locus_tag="MBZ71_000235"
     CDS             complement(12976..13833)
                     /locus_tag="MBZ71_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4428218.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(13818..16520)
                     /locus_tag="MBZ71_000236"
     CDS             complement(13818..16520)
                     /locus_tag="MBZ71_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBT4428219.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(16533..17822)
                     /locus_tag="MBZ71_000237"
     CDS             complement(16533..17822)
                     /locus_tag="MBZ71_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBT4428220.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            17972..19018
                     /locus_tag="MBZ71_000238"
     CDS             17972..19018
                     /locus_tag="MBZ71_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4428221.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            19020..21173
                     /locus_tag="MBZ71_000239"
     CDS             19020..21173
                     /locus_tag="MBZ71_000239"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBT4428222.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(21646..23097)
                     /locus_tag="MBZ71_000240"
     CDS             complement(21646..23097)
                     /locus_tag="MBZ71_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4428223.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(23094..24818)
                     /locus_tag="MBZ71_000241"
     CDS             complement(23094..24818)
                     /locus_tag="MBZ71_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4428224.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(24811..25425)
                     /locus_tag="MBZ71_000242"
     CDS             complement(24811..25425)
                     /locus_tag="MBZ71_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBT4428225.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(25770..27227)
                     /locus_tag="MBZ71_000243"
     CDS             complement(25770..27227)
                     /locus_tag="MBZ71_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4428226.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(27268..28188)
                     /locus_tag="MBZ71_000244"
     CDS             complement(27268..28188)
                     /locus_tag="MBZ71_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4428227.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(28200..29147)
                     /locus_tag="MBZ71_000245"
     CDS             complement(28200..29147)
                     /locus_tag="MBZ71_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4428228.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(29626..30345)
                     /locus_tag="MBZ71_000246"
     CDS             complement(29626..30345)
                     /locus_tag="MBZ71_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBT4428229.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(30394..32040)
                     /locus_tag="MBZ71_000247"
     CDS             complement(30394..32040)
                     /locus_tag="MBZ71_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4428230.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            32219..32809
                     /locus_tag="MBZ71_000248"
     CDS             32219..32809
                     /locus_tag="MBZ71_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4428231.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            32901..34406
                     /locus_tag="MBZ71_000249"
     CDS             32901..34406
                     /locus_tag="MBZ71_000249"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBT4428232.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(34490..35842)
                     /gene="celB"
                     /locus_tag="MBZ71_000250"
     CDS             complement(34490..35842)
                     /gene="celB"
                     /locus_tag="MBZ71_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBT4428233.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(35842..36360)
                     /locus_tag="MBZ71_000251"
     CDS             complement(35842..36360)
                     /locus_tag="MBZ71_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428234.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(36376..36702)
                     /locus_tag="MBZ71_000252"
     CDS             complement(36376..36702)
                     /locus_tag="MBZ71_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBT4428235.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(36715..38694)
                     /locus_tag="MBZ71_000253"
     CDS             complement(36715..38694)
                     /locus_tag="MBZ71_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4428236.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(38715..39029)
                     /locus_tag="MBZ71_000254"
     CDS             complement(38715..39029)
                     /locus_tag="MBZ71_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBT4428237.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            39197..39490
                     /locus_tag="MBZ71_000255"
     CDS             39197..39490
                     /locus_tag="MBZ71_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBT4428238.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(39568..40680)
                     /locus_tag="MBZ71_000256"
     CDS             complement(39568..40680)
                     /locus_tag="MBZ71_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4428239.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            41067..41246
                     /locus_tag="MBZ71_000257"
     CDS             41067..41246
                     /locus_tag="MBZ71_000257"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428240.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            41440..41610
                     /locus_tag="MBZ71_000258"
     CDS             41440..41610
                     /locus_tag="MBZ71_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4428241.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(42034..42981)
                     /locus_tag="MBZ71_000259"
     CDS             complement(42034..42981)
                     /locus_tag="MBZ71_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HBT4428242.1"
                     /translation="MQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQ
                     APTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQ
                     DANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAV
                     VELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDG
                     LAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTS
                     SGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        12907 a         8715 c         7734 g        13639 t
ORIGIN      
        1 agcataatta aaaataaaaa tccaagtaat ttttaacgtg tgcaatcttt tcatatcaaa
       61 gatctattga cttatcgtag ttatcgtaat atgatgaatg cgttaacaaa aagtgaaaaa
      121 gtaaggaaaa tgatggaatg gtaacgatca aagatgtcgc taaagcagct ggcgtttctc
      181 atacgacagt ttctcgtgct ttgaataaca gcagtttaat aaaaaatgaa acaaaacaaa
      241 aaatacgaca actagctaag gaaatgaatt attcgccaaa ctatagtgca aaaggtttag
      301 tgaatcaaaa aaaatatcta atcggactat ttttctcaag tttgcataaa ggaacttcat
      361 caaatttcct agcagattcc attgcaggca tccattcagt attagacaca aattacagtt
      421 tatctgttga aagtatcgat gttgttgatt taacagaaat caacctgcaa cgctacgatg
      481 gactgatcgt tatgagtcaa agtgatgatg atcagccttt catcgactat ctcaaagaaa
      541 aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga tccagcggtt atcaacgtca
      601 ctgccgatga tgcggccggt gttatgcagg cagtagatta cggtattgga tgcggacata
      661 cgaagatcgc ttatattggc ggaaagaaaa attttcgttc gacaaaagaa cgggaaaaag
      721 gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa tccagcctat tttttaacag
      781 gagattacag cttagaaagc ggcctccaaa acatggaata cctattatca ctttctgacc
      841 tgcctactct ggttttttgc ggcaacgacg acatagcgat tggtgcattg agagctgtac
      901 atttagctgg cctccgtgtt ccccagaaca tttctctgat cgggttcgat gattctccag
      961 tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa gcctttgaaa gaaatgtgtc
     1021 aagaaggaac aaagctttta ctagatttga tcgaaggcag gacgatcgaa caatctaaaa
     1081 ttgagctctc caccttgttg atccttcgtg agtcgatcaa ttacttgtaa actacaagta
     1141 attttatata aaaatgttaa cgtgtgcata acacagaaag gaagtttata tgaaaaaact
     1201 gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa gttcttcagt ttggtgaagg
     1261 caattttatg cgtggattta ttgattggca gattcagcag ctgaataacc aacaattgtt
     1321 taaaggtaat gttgcaatca ttcaaccttt agaacaaggt cttggaaata tgatgaacca
     1381 gcaagataat ttatatacgg tcattttaca aggactcagc aatggcgaaa tcgttgatac
     1441 ttccgagatt atcaccgtag tagaacaaat gatcaatcct tatgatgaat gggatgctta
     1501 tctatctcta gcagaaaatg acgacttggc ctatatcgtc tccaatacaa cagaggcagg
     1561 aatccaattc gtggagactg ataatttaac agatactccc ccacagagcc tcccgggcaa
     1621 actgacagcc tttttatacc gacgtttcaa attaaacaaa gctggcttta ccatcattcc
     1681 ttgcgaactg attgacagaa acggcgaacg tctaaaagaa atcattttac gctacgcaga
     1741 aaaatgggat ttagaacctg cttttgttga gtggttagaa aaagaaaatg ttttctgttg
     1801 tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa gatgcaggtg ttttcaatca
     1861 acggcataac tatgaagatc aattgatggt caaagcagaa ccattcatgc tttgggtgat
     1921 cgaagggcct aaagaattgg agaatcaact accattcaaa caggctggct tgaatgtcat
     1981 catcacagat gatatgactc cttatcgtga acgtaaggtg catttgctta atggaccgca
     2041 tacagcgatg gtccctcttg ctttgttggc ggaattggaa actgtagaag aagtaatgaa
     2101 agatccttta ttctccgcat atgttgatca gttattcaat ctagaattga ttcctatgct
     2161 ttctttacct aaagatgaat tagccattta tgctgaccaa atcaaagaac gtttcttgaa
     2221 tccttttgcc catcacaaat tagaagcgat ttcattaaat agtgtttcaa agttcagcac
     2281 ccgtctgctt ccagtgttca agaagtatat cgaagaacaa aatcaggtac caccgttaat
     2341 cactgtttca ctagctgcgc tacttcttat gtatcgaggc gatcaagtaa agccacatga
     2401 tgatgaaaag acgataagcg aatttactga tgcttggtct gataatggaa cagctattcc
     2461 tcgattattg caaaacgctg cgctttgggg agaagatttg tctcaaatac cgaatgtgac
     2521 agatacagtt caagaaatag ctgcaaagat cgatcaaaaa ggtgtccggt cagtattaca
     2581 agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca accaacaatg aaattaaatc
     2641 gacgagacag tgtggtcgtc gcattaaccc ctattccaaa acaaacacaa ctgacgattg
     2701 atcagcaaac gatcaccact cttgaagata tcccgcaagg tcataagatt gcattgggtg
     2761 atttgaaagc tggagacaac gtgatcaaat atggctatcc aatcggtcat gtgacgacag
     2821 acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa aaccaatctt tctggtgaat
     2881 tggattatcg atatgaaaaa gatgtccatc caagtcatta cctttttgag aaccgaacat
     2941 ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg taatgatttg tttttagtac
     3001 ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt caaacagttc aaggaaaaac
     3061 atccagatct gggacagttt gaccatatta cgattttaaa gcatccttat ggctgttcgc
     3121 agcttggaaa agatcatcaa aatactcgag aaattttagc ggacgcagta aatcatccca
     3181 atgccggagg agttttagta tttggattag gttgtgagaa caatacagtt cctgaattca
     3241 aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt tctagtggct caagatgttt
     3301 acgatgagat cgaacaaggt gtggctctat tagaagagct tttaacagcg gcggaaaatg
     3361 accagcgcat atcagtcccg ctctctaagt tgaatgtcgg tttgaaatgc ggcggttctg
     3421 atggtctatc gggaataact gctaatcctt tacttggtgc attttctgac tacttgattg
     3481 ctcaaggagg cagcacgatc ttaacagaag tgccagaaat gtttggcgca gaacaagtat
     3541 tgatggcacg agcggaaaat gaagaagttt ttgactcaat cgttgacttg atcaatgact
     3601 tcaaacagta tttcttgtct tacggggaac cagtttacga aaacccatct cctggtaata
     3661 aagcaggtgg gatcactaca ttagaagata agtctttagg ctgcacgcaa aaatcaggaa
     3721 gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat cagaaaacca gggcttagtt
     3781 tattgcaagc tcccgggaat gatcttgtag ctgcctctgc tcttgcttca tccgattgcc
     3841 agttggtctt gtttaccaca ggaagaggga ctccgtttgg ctcatatgtc ccaacggtca
     3901 aggtatctac gaatacaact ctttttgaac gcaaaggtca ttggatggat tttaatgcag
     3961 gcgtcttatt agaacaacca atggaagtaa tcttagaatc gtttgttcaa aaaatcatcg
     4021 ctgtagctag tggagaagaa acgaaaaatg aacaaaatga cgttcgagaa attgccattt
     4081 ttaaaaacgg agtaacactt tgattttcat aaaacagtat aaaaagaaag aaggaagatt
     4141 tatgttttta tctgatgatt ttttattaaa agacgattgg gcgaaaaagc tttaccattc
     4201 ctacgcaaaa aacatgccca ttattgatta tcactgtcac ttagatccaa aagaaattta
     4261 cgaaaacaac aatttcagca atctgacaga agcttggtta ggaggcgatc attacaaatg
     4321 gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc acaggaaatg cttctgattt
     4381 tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt atcggaaatc cgctttacag
     4441 ttggtcacat cttgaattga agcgtttctt cggtattgac ttgttgataa atgaagaaaa
     4501 tgcagagaaa atttggacac aagcaaatga aattttggca gctcctgatt ttcgccgtcg
     4561 ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca acagatgatc ccgcagacga
     4621 cttgcactat catcagttgc ttgctcatga agaaccagat ttgaaggttc taccttcatt
     4681 ccgtccagat aaagcactca atattgaaaa agaaggattt aatgcatggg tcacactact
     4741 ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa ctgatagaag tcttagggca
     4801 gcggatagaa tacttccatc aacatggatg tcgtgtttcc gaccacgctt tagacatctt
     4861 acgctataaa gaagcggatg aaacagtatt agaagagatt tttcaaaaag cacgagctaa
     4921 caaactatta tcttcagatg aaatcgatgc ttatcgtaca gaaacactga tacatttgac
     4981 ccacttctat cattctcata attggacgat gcagctgcac atccatgctt atcgtaactg
     5041 taatacacaa atgttggaaa aattagggcc cgataccggc tatgatggaa tgaatgatct
     5101 ttctttgact attcctctgc aaaaattatt gaaccgtgca gaagaaacgg atcagttgcc
     5161 aaagacgatc ctttattcat tgaatccgaa tgactatcct gcgattttag ctttgatggg
     5221 ctgtttccaa aaagaacaga atggaaaact gcagttaggt tctggatggt ggtacaacga
     5281 tactcgagct ggtatgcgcg atcaattgac acaatttgct gatggtagtg ctttggggaa
     5341 ttttgtcggc atgctgacag attcaagaag tttcctgtcc tacactagac atgaatattt
     5401 ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg cgaggagaag cacctgaaga
     5461 tgaacaactt ttaggaagtc tagttcaagc gattagttat acaaatgcta aaaattactt
     5521 tggctttttt gcctaattga ttaatagtaa cagtaacgaa tcaaaaacag gatagtgaaa
     5581 taacggaatc cgtttatcca cgttttagag aagaagacgg accaaatttg ctccgtcttc
     5641 ttctcttgct gttctgcttc attgcctata aacctttatg aaagaagaga taacatgcct
     5701 actatccata ttgccggtga ttctactgcg gcagaaaaag atgaaaacaa gcgtcccgag
     5761 acaggttggg gagaaaaaat ctctgcttat ttttcacccg agatccagat caataaccaa
     5821 gcaaagaatg gacgcagcac caaatcattt atccaggaag gaaggttagc ccgtctagct
     5881 tcacagtttc agccgaatga tttcttattt gtacaatttg gacataacga ccagaaaatc
     5941 caagaagaaa gaggtacgct tcccttcggt agctatttaa aaaatctgca gatatttttt
     6001 gaatgcgcaa aaaaagcgaa cgtccaaatg gtcttactga catctgtgac gcgacgagat
     6061 tacttagaaa acggaacatt gaatccagat atacttggag actatccaaa agcgatgcgt
     6121 gcatttgctg agaaacacca tattcctctt ttagatgtgt tctcaagatc acaagaattg
     6181 ttccagacct tctcaaaaga agagacaaaa aaattctacc tgcatcttat acctgatact
     6241 tgtaaaaatt acccagaagg actaaaagac aatacccact ttagtccaga aggcgctgat
     6301 aaagttgccc gaataattgt tgaattgata aaagaatccc gtctgcctct tgctaattat
     6361 ttacaaaaag gagtttgaga tgtttccatt aaatttaggc atccgtgccc acgatttaga
     6421 tgttcgaaat cgtgaagaaa tcgttcaaaa agtatcacag cttcacttgt cccatatcca
     6481 atttgcacca caaaaagcat ttcctgaaca acttaaatta actgacatga cacttgggag
     6541 tgctgcctat tttggtgaat acttccaaaa aaacaatatc gaactttcca ttttaggatg
     6601 ctatatcaac ctatccagca aaaatcaaga agttcgtgca caagcattgg caactttcaa
     6661 acagcaaatt gctctgtgct ctgcctatca agccaagatg attgctacgg aaaccggtag
     6721 tgtctctgtc ggttacacaa aagaaaattt tactgaagaa gcttatcaga tcgccagaaa
     6781 ttctatcatt gaattagtcg ctcatgcaga aaattttggc gtaactgttg ctgttgaagc
     6841 agggatcaat catccaatct ataactatca gttagctaaa cgtttaatcg atgaagtagc
     6901 ttcaccaaat ttaaaaatca ttttagattg tgcgaatttg atgcataaag aaaatcatgc
     6961 tgatcaggaa aaagtgatcc gaggtgcctt ggacaattta catggttata ttacagcgct
     7021 tcatctcaaa gactatatca tggaaaatgg aaagatccgc atggtacctg ttggcaaggg
     7081 ctgtttagat ttccgcccgg tactgcatta tatcaaatat gagcgtcctc taatgtacgc
     7141 cactcttgaa gctactcctg agatttatgt tcctgaagca atagaacact tgcagtttat
     7201 ttaccaagat tgttaaggag aagattatgg aggaacgaat acagaaaagt tatcaaaaat
     7261 atcatgcaaa tgcttttcag acattgctgc atctttatca gggaaattat tggcgttttg
     7321 ccatgtcagg actctttttt attatcaaac atcttccatc ttggttaatg ccgatcgcta
     7381 ttgccaatgt aatcaatgca gcttcatcga aagattcttc tcagttgcag gccatttatt
     7441 tcaacgcttt tttgatggct tgtctgattg gtcaaaatat attgacgaat tattttcatg
     7501 taaaatacca tagcacttcg atccgtcaag tagaagctgg actaagagcg acccttatca
     7561 agaaaatcca agaactatct attccttatc aaaaagaatt gcaatctgga cgaatccagt
     7621 caaagatcat ccgtgatgta gaagctatcc aaacgttgtc ctctcaagta tttgtcagcg
     7681 gattaaatat cgctgtcaat ttattcgtag ctttaggtat cacgctttat aaaagtcctg
     7741 ttgttttcat gtttttctta ttgactgttc ctctcgccgc attcgttact ctctttttcc
     7801 gtaaaaggat cagacagtcc aactcagact tcaggcaaga aatggaagag acttctgcaa
     7861 aagtgatcga agtgatcgaa ttagcaccga ttgctcgagc tcatgggtta gaaaaacacg
     7921 aaacaactcg cttaaataag cattttactc aaatctatga aaaaggattc cacttagata
     7981 tcctgcaatc tgttttcgga tcagtcagct gggctagttt tcagatattt cagctgattt
     8041 gtttgatttt cactgggata ttagctttac aagggcgcat ccaacccggc gatgtcgtga
     8101 tgtatcagac gtatttcacg acgatcgtca attccgtttc tggaatgatc acgctgattc
     8161 ctacgatttc aaaaggcatg gaatctatca cttctattgg cgaactgttg attgctggcg
     8221 atgtagaaac ttatcacggt aagaaaaaaa tccaacattt ggatggaaat tttcgttttg
     8281 aggatgtttc ttttctttat ccggactcac tcaaaccgat tatcgatcat ttttctctag
     8341 aggtgaaagc tggagaaaca attgcttttg ttgggccatc tggctctgga aaatctacac
     8401 tccttaactt attgattgga tttatccagc cgacttcagg aaaaattttt ctagatgatc
     8461 agcctttgga tgaactggat atgcgaagtg tgcgaaatta tttagccgtt gttcctcaaa
     8521 ccactttgtt gttctccgct tcgatcaaag agaacatcac ttatggcttg aaaaacgtat
     8581 caaaagaacg tttagatgaa gtcatcgaag ctgcgcaact ttcttcactg attgaccagc
     8641 ttcctgatgg tctggatacc ttagtaggcg aacatggcaa caagctgtct ggtggccaaa
     8701 agcaacgtat ctccattgca cgagcgttga tacgtcagcc aaaaatcatt ttgctagatg
     8761 aagcaacctc agctttagat aaccagtcag agaaaaaaat ccagcaagcc ctgaattacc
     8821 tgacagaaac acccactact tttattgtgg ctcatcggct ttcaacgatc aaagaggcag
     8881 ataaaatcgt tgtgatcgat gaaggaaaaa tcgttgaaat aggtacttac gaaaaactgt
     8941 tagctcaaaa aggctatttc taccgcttga aaaatgaatg acaaaaaata ggagctatga
     9001 caagactttt gtcgtagctc ctatttctga ataaacggtg ttctcctgcc aactcctcgg
     9061 tcacaagtca caatagtcat caaacatgag aagctgtttt ttgaaattgt tccttgtaac
     9121 tcagagttaa acggtaggct cacgacctct tggtaaatgg tgttcaagag acagcctatc
     9181 ggcaataaga agcaattttg tcatcgctca aggccgaacg tctctttcac accctctgtt
     9241 gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac tggtgtcatg atgatacggt
     9301 tcaagtcatt gatgattgta ctgaagcctt gctctttttg catcaagtca ttgattactt
     9361 cttctgattg gattttcaca gccatttctt gtgctttcat tgcatcttcg tcgctgaact
     9421 cttgtccact catttgtttt tcttgtaatt cgatttgcat cgcttggaag tctttgaaca
     9481 gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc ttctttcaac gctttgaatt
     9541 ctggtaattc acggatctca cgttcgattt gattcgcgct atcgtagatg tttgacatgt
     9601 tatgccctct tttcttttag tagttactct ctattgtaac atggggttat tcgttttcaa
     9661 aggggctact ctccttgata tttccgccag aggtctttag ccgcttcacc aattccctga
     9721 acgccttctt tcaccatatc gccgaagttt ttcagccctt cttttgcttg ttcaccatat
     9781 tgattcaagt tatcttgcca atttccagac tctccattag aatttcctgt gtttccaact
     9841 tgatctgcag ctaccacttg tcctcctgtg gcataggcat ctgctacact aaatcctgtt
     9901 tttgaagtat acggaaggat gccttctgcc gctgatttga agactttccc gacaacattt
     9961 ccacttgtcc cttccaaata gtgcagcttg ctggattctt cgtaacccat ccatgtcgaa
    10021 atcactacgt ctggcgtgta accgatcatc cattggtcat ttgcttttgt ggcatcgaaa
    10081 ttagtttctg tggttcctgt tttccctgct atcgtataac ctgcaggatt cgcagcagcc
    10141 gctgttccat ttgaaaatgt accaagaagc atgctggtca tttcatcggc tgtttccttt
    10201 gatatgacct gttttggttt tttgtttgtg ttatcgacga tcaccgctcc agtcgaatca
    10261 acgattttcg tgataagatg cggcgtatac atctgaccat cattggcaaa tgcactgtaa
    10321 gcacccgcca tgatcaatgg agatactcct ttttccagtc caccaagggc taagccataa
    10381 tacttatcag attttgctaa aggcaggccg aattcttctg ctttatcata tcctttttgt
    10441 aaaccgatct catgcaaaag ccatacagta ggcaaattca aactttgtgc aacagcttga
    10501 tacatcggta cttctccttg gtatgtcccg tcaaaattat gcgcatcgta ataactttgc
    10561 ggttcgtctt tcaagatact ggaaggcttg tatccagctt ccagtgctgg tgtatatacg
    10621 cttaatggtt tgatcgttga tcctggtgaa cgacgcatct gtgtagcaaa gctgaatcct
    10681 ctaaagacat gatctcctct tcgtcctact agtgcttgta caccacctgt attcggatct
    10741 aaagctactg aagcagattg gaccatggca ccatctgaag catttgctgg gaataatgca
    10801 ctgttttgat aagtagcttc taattgcttt tgataatcct gatctaaagc tgtatagatt
    10861 ttatagcctt tattcatgac atcttcttca tcaagaccat actcgttaac tgcttcatcg
    10921 atcactgcat caaaataata aggataacga taaccagaat ctgaattatc gtatgtgtca
    10981 ttaagatacg cgctgatatc tgtctggctt tctgcttgtt cttgttcttt tgtgattttt
    11041 ccgttttctt ccattacact tagtactgtg tttttccgat cattcgcatt ttttgggtga
    11101 tcgatcggat tataaatacc aggacctttc agcattcctg ccaaagtagc agcctcagac
    11161 agcgtaacat tcgttgcatc tactccaaaa tactttctgg aagcgtcttg gacaccccat
    11221 accccgttgc caaaataggc attgttaaga tacatggtca agatttcttc tttgctgtac
    11281 ttcttttcga tctcgattgc caaaaataat tccttcgctt ttcgatcaaa cgtttggtct
    11341 aatgtcagat acgcattttt ggcaagctgc tgagtgatcg tacttcctcc gccccctgat
    11401 gttccgccag acaagaccat tcggactgct gcacgggcaa tccctttgat atcaaacccg
    11461 tgatgctcgt aaaaattcct atcttctgtc gagattactg catcttggat atagggagaa
    11521 atcgcgttta attctacata tgttcctttt tgggcgtaca gtgtgccagc ttcttcgttt
    11581 ttcttatcat aaatgactgt cgattggctt aatcctgatt ttaacgtttc gacatttgct
    11641 tgttttgcta aaataaagag gtagatactt gtcactaaga cacagatcaa accaatcagc
    11701 aaaatcaatt tgttcacatg atattttttc cagatgcgct ttcgccatcg atggaattgt
    11761 ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag cagcttttat cttttctaaa
    11821 atgctttgaa aatccatttg cttcttctcc tgtctatttg attcaatcgt cttattttac
    11881 catagtgaaa gagtgttttc ttcctgctta agttgtagtt caatatgttt ttttacgtta
    11941 gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa gttttttctt aaatatgttt
    12001 taaaaatggt acaattagtc tgcgattgca ctgaaaggag tacacttgtg aaaatcacga
    12061 tcactttacc tatgaaccat cctgaaacaa caattcgcga cttgcttgaa aaggaatggt
    12121 tagttcctag aaaggttcga cactttttgc gtatacgtaa aaacgtttgg atcaatcaac
    12181 aacctgcttt gttccatcat gaagtaaagg gcaatgacca gatcacattg ttattcgaag
    12241 aaacagatta taattatcaa gaagttcaat taggagatgc gaagcacatc cgtgttttat
    12301 atgaagatga gcatttgatt atagtggata agcctgcagg aatgaagact catccaaatg
    12361 aaccgacaga aaacagtacc ttactcaatc atttagctgc ttatctcgac actaaacagc
    12421 aacgacctta cgttgtccac cgcttggaca aagaaacaag cggtgccgtt ttatttgcaa
    12481 aaaatccatt tgtcttgccg atacttggaa gaatgttgga acaaaaaatg atctatcgga
    12541 gatatcaagc catcgtatgg ggagaaattg ccaaagatat gaccatcacc aataaaattg
    12601 gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga taaaaatggt cagagcgcct
    12661 tgacacatgt cactgtcaac aaagtacttt ctgaacaaac acaagtctat tgtgttttgg
    12721 aaaccggacg tacacaccag atcagagttc atttagctca taagggacat ccgatcgttg
    12781 gagaccctct ctaccaaaat agaacagctg aacgactgat gctccatgca ttagaactgc
    12841 acatgatcca tccatttact caagaaaaaa tagtcggtaa agctttacct ggattatggg
    12901 aatagaaata aaaaagaaca gagtcttatc agcagataat gataagactc tgttcttttt
    12961 gtgtaatttg tctgcttact tttgatgttc gtgttctgct aacagcaaac taccgataat
    13021 cccactttca ttcggcagtc cccaacgaac gatatattca cttacttgag gtgtttccat
    13081 ataaccaccc atcaattcaa caaattgctg acggattttt tgaaggagat gatcctgatt
    13141 cattacccca ccgcctaaaa taatcttttc aggtgctaac gtcaacgtat agttgatcag
    13201 tgcttgagca atataataag cctgaatagc ccaaacagga tgatcttgcg gcagatcttg
    13261 ccctttgatc cctgtgcgtg cctcaataga cggtcctgct gctaatcctt ctaaacaatc
    13321 tttatgataa ggacaagtac cttcataatg gtcatctgga tggcgtttca cccaaatatg
    13381 tcccatttca ggatgtgcga tccctgaaaa aatttcttca ttgatcacga cacctgcacc
    13441 gattcctgtt cccacggtaa ggtaaataca actatttttt ccttgtgctg ctccttgctt
    13501 caattcccca tatgcagcag cattgacatc cgttgtccaa gcagcaggaa tattgtagcg
    13561 ttccttgatt gtgccaagaa aatcgaattg tgcccaccct tttttgggag ttgccaaaac
    13621 gtatccgtaa ttatcttttg ttttgtcgat accaatcggg ccgaatgagc caatcccgat
    13681 tcccttcaat tcataacgat caaaaaattc aaatacctga tccaatgttt cttctggtgt
    13741 tgtggtaggg atacttaatt tttctaaaat ttctccacca tctgaaaccg cacacacaaa
    13801 ttttgttccg cctgcttcta ttccgccata cattcgtttt cctcctcgaa tccgattgtc
    13861 cgaatctcat aaggttggat tattgggaca accgcttgct tcgttggttc ttcaagcaga
    13921 ttcaatatga cagtttttcc attgtcttta gttatttcca atttttctaa atgactagac
    13981 atattgaaac ctcttactac tactttatca ctaaacttgc tgcgtttcaa agctgtaatt
    14041 gcaaacgttt cactttttat cgttaaatat tgttgttttg aaatcaatgt tccttcatga
    14101 tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt atgcatgcaa ataagtagag
    14161 aatttttctt ctggtccatg tagttcaatg ctgtaattga accgatgctc acctagacac
    14221 tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt ctcccacact gcgaagtagc
    14281 gtcactgcga tttgtccgtc ttctgttact tcatattcat tcaaaccaaa gtttccgact
    14341 gttacaccgt actcttcatc atgaatattg acaaatgctt gctgatgttg cggattcgtg
    14401 gggttttccc aacttttaga cacatggttt gggcgcttca ccacttcaaa aatactatct
    14461 gcttcgtgat gttccacatg taactttgta ggaaacaaga cacgtaagcg atgatctttc
    14521 atctgattgt cgagagtcgt ttcaaagtcg atttttttac tatcttttct gattgtcatg
    14581 attgttttta tttgaaggat tcgtttttct ttggatcgtt ctgcttttcg ataacgaaat
    14641 tccacaacca tctgctgttc tttttctaaa cgctcgtctg cagaaacagg gatttccatt
    14701 tcctgtagaa tacttatttt tgctatttcc ggtgtatcta ctaatacttt tacttcagag
    14761 ttttctttgt tactggatag gattggctga tcacagaaag gttgtttaaa gatatactca
    14821 tttcctatat cgccgatatc ttcaaaaata tgcagattat tcaactttcg tttgtttttg
    14881 cgatcttcca ttgtaatggt accatttttt tcaatcgtca aatgtagagg gccgttttcg
    14941 atgattcgtc cactctgatg aatcattgtt tccttttctt ctgctttcgc ttccccttct
    15001 acaagcgcga atgtttccca agaaaatgct ggcatgtttt ctaatggtaa agtcactttg
    15061 atcattcttg ccatatatgg cacacggaaa cggtctttcg gcaaatcata accaaattgg
    15121 acagtttctt cagataaaat agctggtata gtggccccac tttttgtttc tacatgatac
    15181 ttacgtttag gcaaattttc taactcttga tatagttgtt caggtattcc ttcagcgaat
    15241 ctttttcttt ctaattcgat ggttatttct gcttcaccag ttttatccat tcctgcagta
    15301 tttacaatga caaatggaaa gctgtctttt gggaatccgg tcgtatcgat tgctgcggtc
    15361 agctgttcta aggaatcttg tgcaagatac tttccaactt catctgcttt ttcaaaacga
    15421 ggtatcattt cccgatggac agaatcgact gaacaaccgc aaatactgtc gtgaggatga
    15481 ttttgcatta atgtcttcca agcataatct aattgatcat ggggatagtt tccactaact
    15541 tcataagcca tcgtcgcaag tggttctgta atattttcta gctgtcgctg gaccttggta
    15601 ttccattgtt ttaagtaaac ccgagcagat gctgtattgg ctaaagtata ccagccatcc
    15661 gtttcttgac ttgtcaattc tccttctact gagcctaagt cttctggcac atctttttga
    15721 actgcttcca gatagtcagt aaaattagaa tgaataaact cgtaatctgg aaacagttca
    15781 tttgcgagat ggattgcttt tgatatatct ttttgtaccg gttgatgatc aacaccattc
    15841 atcatcaata agtggttcgt agaggcatat tgttctgcat ccgctaattt ttgcttccaa
    15901 aaggcaagag ctgcttcttt ttctactgga atctcattcc cgttgctata ccaattggca
    15961 aatagcagac caaagattgc tgtttgatca ggtcctttcc accacatctc tgagtattgg
    16021 gaagaatagt tttctgcttc caaaacttga ttatcaaagc caattggttt tacaccacgg
    16081 ccaaacgctg ctgctgaaat accagcttgt ttcattaatt gaggcgtctg tcccatattt
    16141 ccaaatgtat cgggaaaata gcctaacatg acaggggttc cccatttacg gctttcttcc
    16201 atgccaatca gcatatttct cacgttcgat tccgagctga taagaaaatc atcttgcaag
    16261 atataaaatg gtccaattct caattttcct tcattgattg cttgttggat tgcctgtctt
    16321 ttttctggcc ttacttgaag atagtcatct aaaataatcg tttgcccatc caaatgaaaa
    16381 ctgttgaagg acggatcaac ttcaaatagc tctagtagat cgtctatcaa ttcaatcagt
    16441 cgcatgtgat gctgttcgta agccatgtac cattcacgat cccagtggct atgagaaatg
    16501 atatatactt tctttttcat ttacgatacc tcctactttt ctactctaat atcaaagtaa
    16561 tccatgacta attcgcaaaa catcatattt gcccaagaaa accattctct cgtataattt
    16621 tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg ttcctgcgtc agttgctaca
    16681 agtaaattca atatccgctc tttttcagat ttgtctgacg ttgtcattcc ttccatagct
    16741 aatgcaatcg gccaaacgta tttttctggc gtatgggagg atccaattcc ttttgcgtac
    16801 tttccttcat agaaatatgg attttcttta ctcaacaatg ttcttcttgt ttgcagataa
    16861 tcttcctctt gttcagaaca atatcccaag taaggtgcgg cgatcagatt gggcacatta
    16921 ctgtcatcca tgactgtggc gtttcccatt ccatctactt catatgcata aatcgtttgc
    16981 cctttttgat taaccgttcg tccgtgattc tgaatcccct cttggatctc tttcttcaat
    17041 ttctgtgttt gctgtacaat ttctttatca tcgagtatcg tttgaaaaat ttcttccaca
    17101 taacctaaca cgaccaccgc aaacatattg gatggtacca gatatccata acggcaagta
    17161 tcatcgctag gacggaaacc agaccatgtc attcctgtaa aagctgtttt tgcacctcgc
    17221 ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc gtacaaatgt ataaggagag
    17281 tgttcatgat cttgctctgt tttgaacacg tccaagattt ttttgatacc ttcaataaaa
    17341 gattgattga attgatcagt ttttcctgta tttttgtaaa gaagataggc caactgtatt
    17401 gggtagcata aagaatcaat ttcatatttt ctttcccaga tccaatcgtt catttttgta
    17461 tgatctgttt ggtggccagc accgttagct tcttcgttga aggcattcgc atatggatcg
    17521 atattgatat aaaaaaattg ctttttgact aacccgctga tcatgtcagc taattcctca
    17581 tcttcttttg caatcactaa ataaggacga acttgtgcag tagagtctct taaccacatc
    17641 gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat tttgcttttt gactgttgtc
    17701 aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc agtccgcatg gatatcccca
    17761 catttttgtg tgatatcttc cataaatttc tgcaccgatt ttggtatatc tttataagcc
    17821 actaataaaa ccttctttct atttgatata tctttaaaga ttatacttca tacagccaat
    17881 tatgtctacg ctttcaaaaa atatcttttt atttactttt tcatccatta tggtattatt
    17941 tgaatatgat atatcattaa gaggtgttta tatgaatcaa ccattgtacc agacaatatt
    18001 taatgatttg caaacggcta ttttaactgg tgaattacct gtagacagcc aactaccaac
    18061 tgaaaaagaa ctttcacgta cctataaagt aagcagaatc acttccaaac gtgcattgac
    18121 tgaattagag caattaggac ttatttatcg cgttcgcggg aaaggcagtt tcgttaaatc
    18181 cgcttctccg gaacagattc ttgcttctcc tacaaaaaca cgacggattc tgtttcttct
    18241 tccatatgtc gctgatttgt ctgttggtga ttttagcaag ggattgaacc ctgtgatgca
    18301 agataaacag attgatgtca tgatgacgac gcttgacttt ctacaaaata aaacagcaaa
    18361 agagctaatc aatgaatttg acggattgat ttattatgct cttgatacag aacaacatct
    18421 tgacttactt tttgagcttt cattgaaaga attccctgtc gtcatattag ataaaaaaat
    18481 ctatgagtta ccttttccta ccgttctttc cgataatttc caaggcggtg cgcttgctgc
    18541 ctcttatttg atcgaacagg gacattcaaa aatcgcttat ttgtttggta cgcagactca
    18601 tccacaatcc gttcgacagc gttaccttgg ttaccttgaa gcattggaca aagctggttt
    18661 gactttccac acttcactgg atgacaaaag cgcgacatta gatgaattgc tttcttatat
    18721 cactaagtat gaagtaactg cttttgtttg tgaaaacgat gttgtcgcta tccagaccat
    18781 gaatctcttg agacagcatg gttatcatat ccctgaagat ttttcaatta tcggatttga
    18841 tgatatccaa gcagcagctc ttgttgaccc gccactgaca accatcgcac aagattttga
    18901 acagttaggt aagcttgcgg gagatgcttt ggtcacttgg ttagagacaa aagatcgacc
    18961 atccgatagt aaatttccag tttcattcat caaaagacaa tcaacaaagg agaaataaaa
    19021 tgaatatcgg tcaaatagat actagacatg caacagcgaa tcagccaaat ttctctaatg
    19081 ggaactgcca gccttatact ggcgttcctt ttgggatgaa ttattttgca ccgcaaacta
    19141 cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg cgtttttcaa ggctaccgtc
    19201 ttacccatca accaagtcct tggatgggtg atttcagtca tatgctgttt acgccgatca
    19261 atggaaaact acaggagaac acccttttcc atgctcaaag ttcttaccgc ccagaagaaa
    19321 gtatattttg cccgactcat ctgtcgatct gtcaattacg ttatggcatc tgttctacat
    19381 tgatacctag catgtatggc ggaatcttat ctattgatta cagcaaaaat gacagcggtt
    19441 tacttttatc ctttccggga cgccatcagc tctttgttat agatccttac acagtggaag
    19501 gaaaaatcac acattttgct ggttcagaag atcctgattt ttctttttat tttatattga
    19561 gatttgaata tcctttaaca attgatgagt tagctgtttc caacaaaaat agcgactcga
    19621 tcccgttgta ttttgaacag acaaaaaaac aaactatccg atttgggaca tcttttatca
    19681 gctcggaaca agcagcttat aacctggaac tagaacaaaa ccaaagcaaa gagacttacc
    19741 ttgctcacag tcaagaacaa tggcagtcct attttgatcg tatacagctc acacaccatg
    19801 atcaacaaca attgcggacg ttttatcata atttatatcg ggtgttttta tttccacaaa
    19861 cattttatga aattgatcct agtgggcaaa aaatccacta tgatacgact agtcgatcta
    19921 taaaacctgg tgttctctat acaaataatg ggttttggga tacgtataaa acggtttatc
    19981 ctctttattc tttgattgct gtagaaaaat acgaagaaat gctggaaggt tttttgaaca
    20041 gctatagaga aagtggctat ttgccaaaat ggctgtctcc tgatgaacgt ggacttatgc
    20101 ctgggacatt gatcgatgcg gtcattgcag atgctgctgt aaaagatatc cgacgagact
    20161 tgatgcctga atttctaacg gcaatggaaa aaggtgctac ggttcaaagc aaacatccaa
    20221 attatggacg ccaaggaaca gaagattact tgacatatgg ctatgttcct aatcactacc
    20281 acgaatcagt caatcacaca ttagactatg cttatagcga tttttgtatt agccaagttg
    20341 ccaaggtact agaagaagag gaaatgacga agaaatatca gcagcaatca ttgaactatc
    20401 aaaatatctt tgactatgag tcaggattta tgcgttcaaa agataaagat ggcaagttca
    20461 gagaaccatt ttcagctaat cgttggggac aagattatgc agaaggaagt gcttggcaaa
    20521 gcagctttgc tgtttatcac gatttttcag gactgatcgg aaaatatggt ggcagtgaga
    20581 gatataaaga aaaactcatt gagctgtgca atcagccccc aacgtttgac ccaggaggat
    20641 atggttttga gatccacgaa atgagtgaga tggccgcgat agaatttgga caattagcta
    20701 tttcaaacca gccaagtttt cactatcctt acttgttcag ttatatagga caacctgaaa
    20761 tggcacagcc actgcttaaa cagttgatga ctcagacctt cgacgattct ccaatgggtt
    20821 accctggaga tgaggacaat ggaagtatgg ccgcttggta tatcttcaat agtctgggat
    20881 tttatcctgt cacgcctgga accggtgaat atgttatcgg tatgccatta attgacaagg
    20941 ctgttttgct tttgtcaaat ggaaaggaat tgacgattga agcaacgcct aatcggccgc
    21001 agcaacaatt tatccataaa atcaaacgga tggacaaaga atatacgaaa ttatatttta
    21061 cccatcaaga tttgctgaaa ggcggcacga tctcttacca attagggatc atccctcacc
    21121 ctaagaaata tcaagcaaaa gatttgcctt tttccttatc aaactataat taacaagagg
    21181 ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aagcaaaaat agatcctcat
    21241 atttttcgtt tggttggtct tattaccgca gatgcaagct tttgaacacc gtttattgag
    21301 aggttgtgaa atcagcgttc tgacctgagt attagagaga caaatggcaa aatagttcct
    21361 catattttgc cattttttga gctaataccg agggtcagct gattgaacac cgtttagtgg
    21421 agaggtcgtg aaaaagctgt ttagtttcga gtattaagaa aaattttgtg aaaatggctt
    21481 tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa ctccgtttat
    21541 cgagaggtca tgaaaaagct gccttgcctc aagtaataag aacagcccaa aattagattt
    21601 ctagacaatt ttttataagt tatttcatta cattggcttt ttcttttatg attctccact
    21661 gttttctcgg tactctttag gggatctgcc gacgattttc ttgaataatt tagagaaata
    21721 cgtggtgttg ttgtagccaa tctcacttgc tatctcattg atgttttgat tactgtgcaa
    21781 aagcagttgt ttcgcttttt ccatacgtaa atggttcaaa taagctgaaa aacttttttt
    21841 cgtttctttt ttgaacagct gtcctagata catgacattt aaatgcaggt tttctgacac
    21901 ttctttgagt gtaagtggtt cttgataatg ctggtgcagg attacgatta ctttttccac
    21961 attgtcactg tactttttct gtctctgatg ctttttgatg aatgacagaa gtagctgctg
    22021 aagttcttgc accgtttttg cctgatggat tttttcgatt ccttgtaaat attcctcatc
    22081 ttccagatca atcaattccc gttgaatctc cataaacagt aaaaaactgg aatatcgtat
    22141 atcttccggc atcatggctg ctaactgaaa ctgttcaaaa aaatcagtga tcattttttg
    22201 agcgacttcc aattgtttat tttgcagcac gcgtctgaaa cttgaaaaat cgatggtttg
    22261 atcttttgtt tctgcttgta ctgcgtaaaa aacatgctcg tctttatctc cataaaattg
    22321 atgcaactgt agattgtctt tcgcttgttg gaatgtttca gggattttgt ccaaagaatc
    22381 tgtctcttcc ccgatactga tcagccattc ctgatcagga aagttttctt gaacaaaaca
    22441 acagaagcca tcaacttcat ctgcctccaa caacaacgtg atcaatacac ggtctccgta
    22501 atttcgttga tagtaaaaga tttgttgccg ttcttgcaac catctattga taagagtatc
    22561 atgagttctt gccagttgaa tcaatactat cctatgctgc ttctcactta tttgtcctat
    22621 tatttcttct tcacttattt catccaattc ttcattcaac cattgactga ataacagttc
    22681 ttgatagatt tcttgttgat ttttttgttc attttgctga tctaaccgtg ttttgatttt
    22741 cttgaggctt tcaaccaatt cgaacttatt cacgggtttc atcaaataat tcacggcgcc
    22801 taattgcata cctcctttca aataatcgaa ttcttggtat cctgataaaa tcataaattc
    22861 aaaattatga cgttcttttt gagccgcttc aataaattcc agtccattta tttctggcat
    22921 tgtcacatcc gttactacta aatcaatctg tcgctgttca agtacagcta gtccttcttt
    22981 ggcattctct gctgttgcta ctatttgaaa gcctaaactt tgccaatcaa ttatcttttt
    23041 taacccattc aaaatcatgt attcatcatc taccaataac acttgataca cgctcatccc
    23101 tccttgaatg taatagaaac ggtcagtcca cctgtctcat tattctcaag cgacatataa
    23161 taactgctgc cgaagctggc acgtaaacgt tcattcacgt tttgcagacc gatagaaccg
    23221 tgaagttcca ctttaggatg agacagtttt tcttcaatct gttttagccg tttttcagtc
    23281 atcccttttc cattatcttt gatgatgatc cttacccgtt tgccttcctg caagactttg
    23341 acacttagcg cattatcgaa tcgagtaaaa tcaataccgt gtttaaaata attttcaact
    23401 aatggctgga tcacaaattt aggtacttcg atcttttcca aatcaggatc gatcataaaa
    23461 tgataggcta ctcgattagg ataacgcatt tgatacaaat agacatattt ttcgcaaaag
    23521 tccagttctt cttttaaagt gatcgttttc tcttgattcg tattgtttcg aagcaaagcg
    23581 gaaaacgcat acacaacatc tgctaattct tcacttcctt cgctgattgc atacattcgg
    23641 atatattcta atgtattata taaaaagtgt ggattgatct gtgcttgaag cgcccgcata
    23701 tgtgcgtctt gctgtttgat ttctaatttg tagatgtctt ctacaaactg attgatgcta
    23761 tccaacattt cgttgatacc gattgaaagt tccttcagtt catattccgt gtttgttatg
    23821 tcgatccgcg tctctaaatt ccctgctgct gtttgatcca tggcctgcat gacgatagat
    23881 acttgctgtg aatatcgttt gaatgtttta taaagaaaga taagcagtat attgtctaaa
    23941 ataatgatac caatgatcag cggggcaaga ttttttcccg tttcgatttt tacacttttt
    24001 ttatctagta cagctaatat acgataatca tcagacagtt cttggtgttg aacaaaattg
    24061 tttttttcca gatcgtcaat cggcaattgt gaatttttct tcagactctc tgtgatgatc
    24121 atttcttgtc tttcaaacgt ttctttagct attttattat catggaatgt gtatagtcga
    24181 tttgtcgttc cagaaatcat gtataaagag agtccattga atgacgttag attttctaaa
    24241 atcatatcta gttctttctt ttgaaaacca ataaaaatac ttcctgactc agctcctcct
    24301 tgtgtgatag gcaacttgat ataaaaggcg tctagtaatt ttggtgtccc cttttttatt
    24361 tccccacctt tgttttctct agaagattca taatactccg gtagttgatt caacacgata
    24421 tatatcgcac ttagttcttc ataatttgcg tagagatctt tgatttgatt tgggaaagaa
    24481 actaattcat cggtactgtt ttgctgttcg tatacataca tcagatactg atctatgggt
    24541 tttgttaagt aggtcgtcac attttcgatc ttttcggaat tccctacaag ctggtttttg
    24601 atgatcctac tcaaggttgt tttatcattc agcattctgc ttgtgctttg aacggcatca
    24661 cttgtcatgt tttctgcctg tttttgattt tgttcccaag tatggatact aaaaataacg
    24721 gtagcagtca tcgttacacc gatcatgata accgcgtatt tccgcaacag acgatttagt
    24781 aaggttcctt ttgaaaatag aatccgaaac ttattcataa ctttccaact gtttatctaa
    24841 ttcttcacgc caatgtgttg tcatcgtacc attccaaacc gtcaaaagac caatgacacc
    24901 aaacaggatc aatcctttga attgccaagt cacgcctaaa attataccgc taccaataat
    24961 tatttttaga aaagacgaaa agctggaaaa aaccgaaata aaacttagct ttagcagatt
    25021 aggaacagag atcgtatatt cactatctaa aatcatactg tattgataag tagcaaatag
    25081 gcagagcaag gcaaaaagta aaataaaatc aatagctaaa aaaatgagcc ctttgatttg
    25141 aacagataaa tataaattgt aactcaataa agcagtacca gataaaaata aactaaatag
    25201 cagattgcct cttaaaaaat ttcttttata tatattccag cctcttttta ttgtgatttc
    25261 tttatactcg aatccgtaca aataaaatag ttcattgact gttttccatg ccgcacctaa
    25321 tccaaagatg atcccgccgg caaatgtata tatccagaaa tacaaggtta gttttataat
    25381 gacccagatc cggataaata actcttccaa aaccttccct aacatatctt ctcctccaaa
    25441 cattaagttt atactttctc attatacaaa aaaaggttgg gcaatcagca ccttgacctg
    25501 attattagag aaacaaacgg aaaaatagtt cctcatattt tgtcgttttg tgagcgaata
    25561 ccgagggtta gctgattgaa caccgtttac caagagtttg tgaaatagcc gcttagtttc
    25621 gagtagtaag aaaaattttg tgaatatggc tttccacatt tttacatata tttagcttaa
    25681 taccgagaaa ctggcttttg aacaccgttt attcggaaca agaggttgtg acacgtcttt
    25741 tgtcacaacc cctttgtctt tattcattat tattcttgtc ggaactcatc atattgtttt
    25801 tgcatttctt tcaatacctt atcatagcca gccttgtcca aagcgtcttt caatttcggt
    25861 aatgtttcat ctggatcaac agtccctgta ttcaatccat ctaaatattg attcatgaca
    25921 ttagaaatat tacttaattc tgttttgaca ctgtcagtgt tgaagctgaa tcctaaaatc
    25981 ggtgaagttt ctgcatcagc aatcgattga tcacgtttgg caatcatgtc atccgtaata
    26041 gactcttgtg tatatagaat cttgttgttt cctgtattcc aagcagacat atgtgtattt
    26101 ggctgataac catctagcaa tttgatttta tcatctgaac catcgacttt ttcccatgct
    26161 tctccctcga tcccccaaac aagcccattc aataattctg gatcactgtt aagcagccct
    26221 agaagttcta ctgatttttc tttatttttt gaagtatttg atacgacaaa atttgccatt
    26281 tgtgcttgag ctgtagattt tagtggaaca gtaattgctt ttgagacaag ttcttgtttt
    26341 gctgcatttg tcaaaattgt atcgccataa tcatacggtc cttgtgtttc tccacgcatt
    26401 agccaagtat ttccttctag tggataatcc ttattgctcg ttgcagcgtc acttggaatg
    26461 agtccttgtt tgtaccagct atgcattgta cgtaacagtc cgatatacgt ttcattatcg
    26521 tattggttga tgattttcga atcatctcca tccagatcaa tagcaaatgg taacgtattc
    26581 cctaaaggat aatcaaagtt cccttggact ttgaagcctt gtccaatggc aaaagctgcc
    26641 gtgtttggtt ctttttcatg gaattccttc aagacttttt ctgcatcagc gtaagattgg
    26701 attccatcaa ttgaaagatc gtatttatct agtaaagctt tgttaaaagt aagtacttgt
    26761 tgagcaaaga catttccatt gactgggaaa gcatataatt ttccattgac cagatttcct
    26821 ttaatatatg cttcatctaa atcattataa gctttttcag cgtacttagg cgctaattca
    26881 gtcaaatcag caaaggctcc tttttgggca ttggaaacat agttgttcgc aaaggcgata
    26941 tcataatttt ccccggaaga aatgatgacg ttcattttct tttcgtaatc gccccagcca
    27001 atgtaattga tattgacttt tacaccgatc tctttttcga ttcgtttgtt tgctacttcc
    27061 atcaattgat cgaaattatc tggtttatct ccgatttgat acatctgcaa agtgtccgaa
    27121 ttgctagctt ctgcctggtc tttagaacca gaacctccgc atgctgctaa tgttcccaac
    27181 aacaacgctg aaacacctgt aaaggctagt ttcttccata acttcatttt tcttcctcct
    27241 aattttatga tttatatttt tgtttcttta ttcttttact ccgccgattg ttagaccttt
    27301 gacaaaatgc ttctggaaaa atggatagct gatcgcaata ggcaacgtcg agatgactac
    27361 gatcgccatt ctggcagatt ctcctggaac tgaggctaat ccgcctgtca attggcttcc
    27421 tgctcctgaa ttttgtgtca gatattgaat attactttgg attttcatca aaagatactg
    27481 tagtggaact agcttatcat cttggatgta tagtaaagca ttgaaccagt cattccagta
    27541 acccaatgcg gcaaataaac tgattgtcgc aatacctgga acagctaacg gtaaaacgat
    27601 ctgaacaaat attcgcagtt cgctagctcc atcgatccgt gccgattcaa taatcgaatc
    27661 agagaccgaa cgtttaaaga atgtgcgcat cacaataata ttgaagggac tgacagccat
    27721 tggcaagatc aatgcccaaa tcgtatcttt taactgaaga agatttgtca taacaagata
    27781 attggccacc atccctggtg agaacaacat cgtaatcaaa caaaaaacgg taaaaaaacg
    27841 acggaatgga aagtttgacc gtgaaatcgc ataagcatat aaagaagtag ccgtgctatt
    27901 gatcagtgta cctaaaacag taacaagtac agtgacgaaa agcgcttgga aaattttatc
    27961 ctgcatctgg tcgaacaagt acgtataacc aaatatactg aacttttctg gccaaaaact
    28021 gtacccgtga gtcgacaata tggactcatc ggtaaaagag atggcgataa caaatataaa
    28081 tggcaaaata caggaaatag caaagagtgc aatcattata ttagagaaaa aattagccgt
    28141 cttattgaaa gagcggactt ggactctctc cactttcttt tttttcattt ttttcctcct
    28201 tagaataatg cagaatcgct atcaaaacgt cgggcgatcg tatttgtgat catcaacaag
    28261 acaaagccga caactgattg gtaaagtcct gcagcagccg tcataccaaa atctcctgtg
    28321 gatgtcaggc cattgtagat atacgtatcc aaaacttgag tcacatcata taatgcaccg
    28381 gaattcctag gaatgttata aaacaggcca aaatcagctc ggaaaatgtt tcctacggct
    28441 aaaatcgtta aaatcgtcat tagtggaata agttgtggta tcgtcacatg tttgatctgc
    28501 tgccatttgc tagctccatc aaccatagca gcttcataat aagtcggatc gattcccata
    28561 acagatgcaa agtaaataat gctgttataa ccaatccctt tccatgtacc aatgaacacc
    28621 aagataaacg gccaatattt cggttcatta taccaattga cagcttctcc tccatgtcca
    28681 gtgatccact gattgaatat tcctttgtca ggacttaaaa aagcgtaaac aaagtaactg
    28741 ataatgaccc aagatagaaa ataaggtagt aaggacatcg tttgataaac tttaacgagt
    28801 cgtttatttc tcaactcgct cataatgatt gcaaatacga ctgagatcag cagattcaaa
    28861 aggataaatg ctacattata taaaattgta tttttagtga tgagaaatgc ttgatccgaa
    28921 gaaaataaaa atttgaaatt ttcccaaccc acccaaggac tttccctcaa gctagccaag
    28981 aacccatctg ctgaaatatg aaaatttttg aatgctacga cattcgccat tacaggtatg
    29041 taaaagaaaa aaatcatcca aagcataccc ggtacagcca tcaggacaag tgctttataa
    29101 cgccatagat tcataaaaaa atgattgaac ccctttcgtt ttttcatcta aaaacccctt
    29161 tcgattcatt cgcaacgtaa ttatagcgct ttcaataatg agaataaata aacaaagtat
    29221 agggaaaatg aaacaaagta taggagaggt tgtgaaatca gcgttttggc ctgagtatta
    29281 gagaaaaaaa cgggaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
    29341 tcagctgatt gaacaccgtt tatcagaggt tgtgaattca gcgttttggc ctgagtatta
    29401 gggagatcgt gtccttacta tctaactctg agtcacaagg aacaatgggg actgtaacaa
    29461 aagtcttgtc acagtccctt attccgaaca aacggtgttc cagaagtagc ttctacggaa
    29521 ataagccgaa atttcacaaa aaagccatcc gaaagtcaga cggacaagtc tgcttttcag
    29581 atggcgctac aactactctg cttattcttt tagctatcca ttcaattatt tttctctcac
    29641 ttgttctagc tcttctttgg tcatgggtcg atattgccct ttattcaaat ctttaggcag
    29701 ccaaaatttt cccatacgga ttctttttaa atataagact tcttttccta ctgctttgac
    29761 cattcgctta acttggtgaa actttccttc atgcaaaatc agacggataa ttgaagtttc
    29821 tgtctcttga tctacttgat caatttggag ttttgccggc aatgtctgct catcgccatc
    29881 aataatcagt ccttctgcaa ataaacggat atcttcttct gtcattatac cgctaacctc
    29941 agctagatat tctttctcca catgtttttt aggtgatagt aactgatggg caaacacccc
    30001 gtcattcgtt aaaatcaaga ggccctctgt gtctttatct aatcggccga ctggaaaaag
    30061 atcttcccga taatcctggt cttccaataa atcgattacg gtttgatcat gattatcctg
    30121 agtagcagaa atgactcctg caggtttatg caaaatgtag taaaaatctt tttgatacgt
    30181 aatgatttct cctaaataag taaccgcatc ttcgaattca tttacttgcg ctttatcgct
    30241 tttcaccact tgtccattga cttcgatcgt tccttttttg attaatcctt tgacttcttt
    30301 gcggctgcca agtcccatat ctgctaaaaa cttatctaaa cgcatgcgtt tcccccattt
    30361 caaaagagca tcaatctagc tcttctgatt cacttatttt atttttaatt tatgtcgtaa
    30421 acgcgctatt cctggtccta gtagtctttc tgccaagcgg attttcaaag ctaaataacc
    30481 atacactaaa gcccctatac ctgctactac aagaatcaac aatagagact ggaatttgct
    30541 atcttcgttt aaaaaagcac caaaaatcat ctttgtgatc cacgaagcca aaagcataat
    30601 caaggtaaga ataaagatca gcaatgtcct gcgccagaca aaacttggtt taaagcgggc
    30661 tacttcatgg attctcttta agatcaaggt acaagacaca gcaaatccga tcattgtcgc
    30721 caataaaggg ccgtatactt caaataaacg aatggctggg tattgaagaa ggagtttcac
    30781 tagaaaccca ataaccaaat aattaattgc cgccttgttt tcaaacattc cttgcagcat
    30841 gttggaaact agcatatata gtcctaggaa gagtcctaca aaacttgctt ggatcagtac
    30901 attacttcct agagaatccg gtgtataaaa taatgtattt agcggatagg caagtaacat
    30961 aacaccaaat gtcgccggaa acataaaaaa tgaaaacaac tgtaaatttc cgcttgtcaa
    31021 ttttgccaga cctactcgat ctttcaacgt aacagcttct gtaatcaacg gtaacccagt
    31081 agacgcgatc gatgttgcca atgcaataac taccatcgtc aacttatctg gattggcact
    31141 aaaaatcgaa aaaagatcca gtaactgtgc attggaatac tcggtggatg cagacatgat
    31201 cttcataaaa gtgaactgat cgactaattt gaagatcgtt acccctgaac ccacaataat
    31261 aaacggaatc gcttctttaa cggtatctaa caacaattcc ttcgttgcaa tatttatttt
    31321 gttttcgctg tagtgaataa acgccgatgt atacgcttga tgtttcttca aaaaatacaa
    31381 aaggacaaga atacttgcta gcataccgat aaatgccgcg aaagttgatt gcgtgaccgc
    31441 cgtcacatag tcaccatcca tgactttcat gatgatgaat gtagccaata gcatataaaa
    31501 aatacgtgcc acctgttcta caatctgcga cagcgcataa ggcatcattt cttgattgcc
    31561 ttgcaaatac cctcgtatca cactcataca aggaaaaacc aatacagcga tactcaaaga
    31621 acgcatcgtt ggaatcagtt cttctccgcc accagaagca tgagctagcc aaggagaggc
    31681 gatgtacatg aacaaagcaa acaacaggcc taacccgccc atcatctgta atgcacggat
    31741 aaataatcgt cgtgaagtgc catattcatt caatgaatta tagcgtgcag tttgttttgc
    31801 aatcgcagca ggtattcctg ctgtagagat cagtagaaac aatgcatata tattgtatcc
    31861 catattgaat aatccattgg cagccttagc attttcgccc atccaagcat accagggaat
    31921 gatatagaca gcgcctagca atcttgaaat gatattactg gcggtcatcc aagcagagcc
    31981 tcgtgccatt ttttcttgcg gtgttaattg atctaatgct ggttttcttt gattatccat
    32041 cggtcagatt ccaactttct acaaaacatt actatcattt taattggtaa aacagaaact
    32101 ggcaatcgat tttatataac ttttataaga acaattgttt ttttgaattc ggtttcgttt
    32161 ttttggtaaa gtaaagaaat atacagcctt ttatggctaa catttatagg agatatcaat
    32221 gacacaagaa ataaaaaaac tacccgttat atttttaggt cttttcttta tcacagttgg
    32281 attgaattgg ttcttgctcc ctcacgatat agcttccgcc ggagtgggcg ccatcggaca
    32341 tttagttgaa acggcctttt ctatccaacg ctcagttact gtttggagca tcaatcttat
    32401 gatgctgctt ctagccgctg tcctattagg aaaatcagta ttttttaaga ctgtagcggg
    32461 aagtctgctt tttcctgttg ttttagagat tgttccaaaa gtggaaatga tctcctcaca
    32521 tttcttttca ctgatttcag gaagtctttt gttcagtctt ggggtttaca cattgtacac
    32581 agtcggggcc tcaaatggcg gtataacgat accgccgatt attttccaca aattttttcg
    32641 gcttccaatg ccgcaaggac ttttacttac caatagtctt atcgtctttt tgaattatta
    32701 tgtttttgga ttgttggaga cactattcgt tctcctatct attacagtca gttcattgtt
    32761 tatgaaaata ctcatccgct tgtcccctgt gtcaagaaaa tttgaataaa gctggatttt
    32821 gtcatagcgg aaacaaacga tatattaaaa agagtgtgct ataataaacc aagaaattga
    32881 ataaagcgag ggatcaatca atgagcttct ctttagaaga catccgtcat ttactattaa
    32941 aagaaaatct attaaaagaa tttgtttcaa caagaggctg gcatcttgat gtaccagaaa
    33001 aagctgaatt tcttcagtta agttatgatt caagaaaagc tgatgcttct actttgtttt
    33061 tctgtaaagg aatgaatttc aaagaagaat atttggattc agcaatcgaa cagggtattc
    33121 aatattatgt atcagaacaa ccatacgaaa attcagcagt aggaatcatc gtaacggata
    33181 tccgtaaagc aatggcgctt cttgctatgg ctttttacga ctatccgcaa aataaattga
    33241 aagtgatcgg attcactgga acaaaaggta aaacaactgc tgcgtacttt acaaaagcaa
    33301 tcttggatca cacaacaaat aaaaaaacag cattgctttc tactatgaat acaacgttag
    33361 atggcaaaac atatttcaaa tcccatttga caacgcctga gtcattagat ttatatcgta
    33421 tgatgtcaga agcagttgaa aatggtatga ctcatctagt gatggaagtt tcttcacaag
    33481 cttacaaaac acaacgcgta tacggcttga ctttagatgt cggaatcttc ttgaatatct
    33541 cgcctgacca tatcagtccg atcgaacatc cgacatttga tgactatttc tattgcaagc
    33601 gacaattgat cttgaattca aaaacagttg ttttaaatca tgaaagtgac tattttgatt
    33661 tacttaggga aacagccgat ctattccaga ttccaacgat cacttatggt cgttcggaag
    33721 aaagcaatta tcaagtgatc cgttcagata aaggtactca cggttttact ttatcttctc
    33781 atgaagatcg cttagctatc tgcgatactg cttatgatat tttgctggct ggaggattca
    33841 accaagaaaa tgcagccagt gcgatcattg ccgcagcact aacaggtgcc tccagtcaag
    33901 atgcacaaga cggattaaaa gaagctcgtg taccaggccg aatggatcaa ttgatccaat
    33961 ccaatggagc gcatgtctat gtggactatg cacataatta tctaagcttg aaaacattgc
    34021 tgaaatttgc caaaaatgaa catccagatg gccgtgtaat tgttgtatta ggcagcccag
    34081 gaaacaaagc aatctcccgt cgacatgatt ttggaaaagt gctatcagaa acagctgaca
    34141 tcgccttttt gacggcagat gacccagcat ttgaagatcc gaaaaaaatc gcggaagaaa
    34201 tcaatgaagc aattactaat ccagatttga tcgttcatta tgaaatggat cgtcctgaag
    34261 cgattcgacg cgctttagca gaaagcgctc cgcaagattc agtcgttatt gcgggaaaag
    34321 gcgtggatcc ctatcaaaaa atcaatggtg tagatgaacc ttatgaaggg gattatgcaa
    34381 tcgtgaagcg gttgattgaa gaataatggt atttatatac tagtggtttc ccatttctaa
    34441 gaaaaaaagg gactgtgatt ttttgtcaca gttccttttt tcttagaaat cattttaact
    34501 tttcactagc taattctgct gccttttctc tttcttcatt taataattta ttatcataga
    34561 atttgataaa tgggaaccag ataatgaatg cgatgattgc acaaatgact gctaacacag
    34621 cagctttcca atcaccacca ctaccaacaa atgctccgat cccgactggt gaaggccacg
    34681 gcatctgtgc aagcatcggt ttgacgattt ccagccgaat ggcaaaataa gcgagtgtag
    34741 cagaagccat cggagctaag aaaaacggaa ccgctaaata gggattgtag acgattggca
    34801 tcccgaaaat gattggttca ttgatattga ataatcctgg tacaagtgag gcttttccta
    34861 acactttcag ctgatcagat ttcgcgagga atgcaataaa gatgatcaat cctaaagtcg
    34921 cacctgatcc accaacggtt acataagaat tgttgaattc tcctgccaac ggataatttg
    34981 cgccttgtgc gttagctgcc atattagcta atacgatcgg ggtcaagaaa gaagtgatga
    35041 tattcgctcc atggatccct acgatccaca atgcatggat caagaaataa atcaccatga
    35101 caccaagcca tgtattcgtc aactgcgtaa caaacccaaa tgggatagca atcattttga
    35161 agatatccgt tcctagtgca actaataatc cattgatgat catcaccaca aacgcaatga
    35221 caaatgttgg gatcagagca gtgaaagaac gagaaactcc tggcggaaca acatctggca
    35281 tttttatgac ccaatttctc ttgacacaca tacaatacaa ttcgaccgca ataatcgaca
    35341 taataatagc tgtaaagatt cctgaagttc ctaaacgttc gacaaaagag cccatcctaa
    35401 taccatttac tacagtttct gtctcattga tagaagcaat caagctgatt ttaccatcag
    35461 tcatgatcaa ttcagggata cacataaaga atgcaaaaac agatagtaat gcccccgtga
    35521 gcggattaac atttaatttt tgttcatctc gttcaattga cgttaattca taagcaaaca
    35581 caatggcaaa gtaaatagat aaaatcccca tcgtcattgt attgacgatc atgtacaagt
    35641 cactaacttt gaaaaaagtt gcattaaaaa atccttctaa aaatgggaac ggtaatggca
    35701 aaatatttaa aaccaaaaac atcgagccta caatcgtgaa aggaatagaa gccatccccg
    35761 ctgccatgac agcccgaacg attttaagtt gcgcaatttt ccccataggt cccatcaggt
    35821 atttctgcaa aaaatcaaac attagttatt ctcctttcct aaatataacg ctttttcata
    35881 ttgtttaata cggatatact gttggtcttg gttgagtgaa attttcttta aacgatactg
    35941 tataaatgca ctggcaaaaa taccgcttac aacaagaatg aaaacaaata actgtcctcc
    36001 attgttattt gccataaaag gatacaagaa tgtaaatcct gaggtaaaca acactggaat
    36061 cactaataaa ttaaagacac tctggaacaa aaaataccac tttgtccatg gaacgttcgt
    36121 ttggtgtttt cgataaagct ttacttgttc tcctactgca ggcaaaatag ctaaaatcag
    36181 aatcatcggt aaactacttc ctaaactttt agctgataga agcagaatca tccattgcat
    36241 gttcatgaaa aagaaaaagg cagtcaggta acgtacgatt agataacgat tgaaatacat
    36301 gtttttcaaa ctaagttgct ttttttctaa ttcgattttc ctttttactg tatcctgcac
    36361 aatgatcctt cctttctatt tatttttttg atacaaataa ttcatttcct gtgcaacttc
    36421 caataatgtc atcgtcgtca tcagatgatc ttgtgcatgg accatgataa tttccatttc
    36481 tatttttgtc ccgctggcat attctttcag aagttgagtt tgtgactgat gcgcatgaag
    36541 aatctcttca tttgcttgtc gcaacttctc ttctacttct tcaaacatct ctttacgcat
    36601 cgagcgaaat gcttcatgta tcagtgtccg tgcatttccg ctatggaaaa tgatatcaaa
    36661 agaagtcact tgtagtgttt cactgtttat tttttcagcc atctgttttc ctccttattc
    36721 gatcagtttg ataaatttac tgctgaaatt ctcaaaaata ggttcagcta agatttcctg
    36781 ctgtactttt agctggtcaa ctaacctaac gattgcttta gtcacgaccg taattccttc
    36841 attttcgaca taagaaggcg aaactaaaaa aacaaaatga atttcttgat attgatccca
    36901 ctgcatccca gaaggaatca atgcaacggc tacctttgtt tcgattccaa ctggaacagc
    36961 gggatgtggg actgccactg tatcactgaa tatgatttgt cccatatttt cacgttgctg
    37021 gatctgcttt cgcatttctc ttatatatcc ttcttcttca tttatgctca gcttttgcag
    37081 caaatcatga agaatttctt cctttttcgg ttcgtacgta taagtcttga aaaatttttc
    37141 agacaactgt tcggtgtaaa tctgcttttt ttgctgtaga gaaaatgctg gtatcggtct
    37201 atctgaggaa tgatagggta tagattctcc aactaccttt cgtattttac gaacatcatc
    37261 ttcattcaaa aaaatactga catgtaaaac tggaattttg aaaaacattg tcgaaagatc
    37321 aatagaagaa ataatcaggt cgattccttt taaagttgtt tcattgatct cataatagcc
    37381 ttttacactt accactgtaa tatttttacc gaattcactg actacgcgat tctttaatag
    37441 ttgggcactg ccgtaacccg ttgcacagat aatcaatgca tgtagtttat gcgcagctct
    37501 ttctttttcc aatgccgcca tgagatgcaa agccaaatag gcccattcat cttcattgat
    37561 ggtatagttt ttcaaagccg gcatgttgct gaaatgcctt tttactgcgt caaagacttt
    37621 gggattttct ttctttatct cctcagtcaa tgggttctca atgcgaatac ctcggctcaa
    37681 tcgaatcatc atcggtgaca aatgatcgag tagtccattg aaaagctgga gatcctctac
    37741 taatgaaaaa tgaaattcct gagacaatct tttcagaact ttcgctaatt cctttgatag
    37801 ttcctgatca ttttgatttt ctttgtgatt cgacttagcc atgagatgta atgtcaaata
    37861 agcaatttct tcttcgggaa acgatacacc tgcgattacc tcaatacgtt gaacgatccg
    37921 ctgcgccact tgatactcta ctcgcttaga aacttcttgt tcgatgccta attccttgat
    37981 ttcaaaccct tctcggagcc gtttgatccc caaagccaag tgtaaagtca gattctgtat
    38041 gataaaatcc gaaactttta acttagcttc tcttgtttca tcgagaatga taatgactag
    38101 ctcttcaaaa ctgatgtctg ggaaaaaacg gctttgccct aaatattctt tcaatgaatt
    38161 tccataatgg tttccaaaaa aagtatccat gataaaatgc cgtcgatttc tttcttcccc
    38221 ttcgatccat gtcccttgac catgcttaga aacgattttt agtgaatatg gaatcaatcg
    38281 ctccttgatt tcttgcatgt cctttgtcaa actggaacga ctgatataaa gctcttctgc
    38341 caaatcatcc ataaaaagaa tggcatcttc caaaagcaat tggttcaata tatacttttg
    38401 tcgatcttcg gactcataaa atccttgcgg ttctctagca tatccaggta ctatttcttg
    38461 ccgactcaaa aacaagtcaa atgtcagtct ttgtaaaata tgcagttggt agccataacc
    38521 tggtttagca atgatcttac ctccgttttt ttctactaac tctttgatcc gctgcaagta
    38581 attacggatt gtccgatcag ataataacaa ttcagaagct aaacgttgac tggtgacata
    38641 ctccttcttg tgatcaatca aatatctcaa cagcaatttt tccttctgtt tcattatcat
    38701 ccactcctat tccatcatat gttctcttcg atcacctctg ctaacttctg gatgcctgta
    38761 gggattggga catacgcttg gaaagggata ctaacaacag gctttccaac agattctcct
    38821 aattttttga atttatccaa aaacatcgtc gtttgcgggc tgatcagaaa taaatcaaaa
    38881 tcactattct taatcatatt atcgccttct gtagctgaaa ctgcatctac gagtacctct
    38941 tctctttttt gcttaaagaa atccgttgtc tttgaggcca tcatcgagga tgacattcct
    39001 gccgcacaaa taatcaatgc ttttttcata aatcaatcac tccttttatt tttataaacc
    39061 aagcatatct tgtaagcgtt ttctattcaa aatatatatt tccgtagcat tgcggaaaaa
    39121 atgtgtatca tatcttgaat aataatttct ggcaccgtga tatactagtg atagaatttt
    39181 ttcaggaggg gatttagtga gtatcacagt gaatattttg tatacaagta caaatggaca
    39241 agccagaaaa tttgcagaag aaatggtcga aagaaacatt gtcaaagcga ttcgtgcaga
    39301 agaaggaaac gagaaatatg actattactt tccactagaa gatccagaat ctcttttact
    39361 gatcgaccgt tggaaagatg aagaagcaat cgaaaagcat cacaaatccg aaatgatggc
    39421 tcaaataaac gaactccgta aaaagtataa attaaaaatg aaagtcgaac gtttcactga
    39481 tttaccgtaa aaagatacac atggaatgat agcaatttat catccatgtg tatcttcact
    39541 ttttccttat tttttattcg atctcatcta atgattcaaa ccgtgttttt ctcttatttt
    39601 tcggataagt atttcagtca ccccaatata catgattcct aaacataccc caaggatatt
    39661 ctcaaaaatg cggatcgtta ctgctcctgg aaccccgaat aaactggcag tgattgccaa
    39721 agcaccaaaa gaattaaaaa ctgttttata tctataggta gaacaaacac ctaaagctag
    39781 accacctaat ataccgaccc agttaaaagg aataaattgg ctgataccaa taaataaaac
    39841 acatccaatc aatgagccaa tgattcggtc tactgctcgt tctttgaaac caatcgacat
    39901 caatccatag ctagatacaa tagaagaaaa tgcaaaccct gcccacatca atcgctgaaa
    39961 agggacatac tctcctgcaa cgatcaaaag actaatgcca aatgcataga tgagcatcca
    40021 aattttttcc tgcgaataca tgcctttcaa aaaaatctct ttaaacaagc ttttgtcttt
    40081 gttcttttct cgatgttttc tgtaaaggag aactgaaaac caacaaaaaa acaaaaataa
    40141 taagctactt gtttgtgtaa agaaattctt gttcaacgaa tctttaggta acgaataaac
    40201 aataaagaga tatgaaaagc cgtacaaaga agcattcccc attttgggat cactagcggt
    40261 tgccattaat atgctgctta gtaaaataaa atgaatcaac atgcccataa ctgaccattt
    40321 gatcagctgg acatacggtg ctactaatag tgagaacatg acgattgcta aacctatgat
    40381 cgaatgggaa atcttataac caaagtcaac aaaacgaata ctcatcaaca tacagaataa
    40441 aaccacagcg tatggtttat gcttcgattc aaataatgtt gtaattataa tgataaaaca
    40501 aatggcaaac aatacgatga ataatgatcg taaaaacaat gaacaaatat acatgcactt
    40561 ttctttttta gaagctgctt ctcgtatttt ttgttttaaa ataaacggat ctagttgtag
    40621 agactgataa aaatccacct gttgtcgtcc tttcttttta cttttatttt ttacagtcat
    40681 ctatgacagg ttgtaaatac tatggaacat catatggcgt tgtcattttc ttgtcaacta
    40741 tttttcctat tcatgttttt ataaaaaaac atatcgtttt cttgtatgat taaatcacca
    40801 caaaataaac aactgagaga acgatatgct ttatgaatgg ttctatcaaa aaaattctga
    40861 gatcaataga caaaaatttg atgattacag aggtctctta cgaaacctta cagaagaaaa
    40921 aaacattgat cgttgctgca gttctcttcc ctgctccatg tgtctgtaga aattgtggtt
    40981 ctgctgtggt agatggaaat aggaaattaa tggtggtgaa aaacaagaag aaggaaacca
    41041 ttgtccactt tgaataatat aatcatatgc caatggttat acgcctaaaa aagcagcgct
    41101 atacctgtaa aaactgtcga agccattgga acgctcaaag ttattttatc cgtccaaggc
    41161 actcgatttc aaatcatgtc agacataaaa tcacttcttt actgactgaa aaagtgtcgt
    41221 tattttttat ttcgaaaagt tgttaggtat ctttgacaac cgttattcgc acattgaaag
    41281 agttgagaag ctatttacca aagtcttcca aaagaattct gtcttgagtg ttgagggttg
    41341 tgaatttcgt tctcacgttt ccgttgagga taaactgagt tttatttccg cggatggcga
    41401 gacagaaaaa ttaatcgaga aaatttacct acacaaaaaa ttcctcgatt aacaaactat
    41461 ttccttagct gcactaatcc taaagaaata gaattcttag ttacagacat gaacgccacc
    41521 tactttcagc tcaccaaaca tgttctacca aatgcgaaat tggtgattga tcgattactt
    41581 ggtttttcca cgcctttcaa ggaggcgtaa cctttattca cgagttggtt gaagctttcc
    41641 gagacaaaag acccgacttg tttttctcct tattgacaaa gagccattaa aactagagaa
    41701 aaaattacaa ctgatcgctg attatttgta gaaagaaaat caaagtatta cccaataaac
    41761 ttgagtcatt aggcaaaaaa cactgcgaaa ctcaagaatg attttcgcag tgtttttgta
    41821 tattttttat taactagtat tactacaaag gtttattttt cacttctcaa cgcagcacaa
    41881 aattgtcttt acttatttac tggttgggtt caaccttctc tactccgata ctaatatttg
    41941 tttgtatacc ccttctaaaa tcggtagttt ccattcaatt gatgggtact agttgatttt
    42001 aaaaactatg cttattcaat tttttatttt agctcatata tttcgtttct ttctaaattt
    42061 tatactgatg aaaacaagta gcacacccaa tattgacaat ataaactggc taatgatggt
    42121 ttctccagtt tttggaagat gcttatttcc actgcttgtt ggattgtttc ctcttatatc
    42181 tgagctatca gcaacttttc tattctcttt tgttaaaaag atcgccactt cttgttcttt
    42241 ttcgatcttg aaagttactg gtgtcgcatc caattcatac cccgttggcg cttgagtttc
    42301 taccagctga tatgctcctg gtgctaagcc atctatcgcc agttttccat ctgctcctgt
    42361 ggttagacct gactgcagaa cggttccatt ggcatcttgt aactcaaaaa cagctccttg
    42421 caggacttct ccgctttggt catcgatctt tgtcaacacg acaccgcctg gtgtaagtcg
    42481 attttctttc gtcaattcta ccaccgctgt ctgacttctt tcgatctcaa actcgattgg
    42541 tgtcgcatcc aattcatacc ccgttggcgc ttgggtttct accagctgat acactcctgg
    42601 tgctaagccg tcaatcgcca gttttccatc tgctcctgtg gttagacctg actgcagaac
    42661 ggttccattg gcatcttgta actcaaaaac agctccttgc aggacttccc cgctttggtc
    42721 atcgatcttt gtcaacacga caccgcctgg tgtaagtcgg tttgctttcg tcaattctac
    42781 caccgctgtc tgacttcttt cgatctcaaa ctcgatcggt gtcgcatcta attcataccc
    42841 cgttggcgct tgagtttcta ccagctgata tgctcctggt gctaaaccgt caattgccag
    42901 ttttccatct tctcctgtag ttagaccggt ttgtagcgtt tctccttctc ggttctgtaa
    42961 ctcaaaaaca gctccttgca ggatttcccc gcttt
//
LOCUS       DAGCWU010000006        41144 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.006, whole genome shotgun sequence.
ACCESSION   DAGCWU010000006 DAGCWU010000000
VERSION     DAGCWU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.006"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            177..404
                     /locus_tag="MBZ71_000260"
     CDS             177..404
                     /locus_tag="MBZ71_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBT4428243.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            420..1586
                     /locus_tag="MBZ71_000261"
     CDS             420..1586
                     /locus_tag="MBZ71_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBT4428244.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1821..2864
                     /gene="hrcA"
                     /locus_tag="MBZ71_000262"
     CDS             1821..2864
                     /gene="hrcA"
                     /locus_tag="MBZ71_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBT4428245.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2958..3521
                     /gene="grpE"
                     /locus_tag="MBZ71_000263"
     CDS             2958..3521
                     /gene="grpE"
                     /locus_tag="MBZ71_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBT4428246.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3572..5401
                     /gene="dnaK"
                     /locus_tag="MBZ71_000264"
     CDS             3572..5401
                     /gene="dnaK"
                     /locus_tag="MBZ71_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBT4428247.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5552..6718
                     /gene="dnaJ"
                     /locus_tag="MBZ71_000265"
     CDS             5552..6718
                     /gene="dnaJ"
                     /locus_tag="MBZ71_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBT4428248.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7086..7823)
                     /locus_tag="MBZ71_000266"
     CDS             complement(7086..7823)
                     /locus_tag="MBZ71_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4428249.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7825..9327)
                     /locus_tag="MBZ71_000267"
     CDS             complement(7825..9327)
                     /locus_tag="MBZ71_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBT4428250.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9586..11580
                     /gene="uvrB"
                     /locus_tag="MBZ71_000268"
     CDS             9586..11580
                     /gene="uvrB"
                     /locus_tag="MBZ71_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBT4428251.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            11590..14406
                     /gene="uvrA"
                     /locus_tag="MBZ71_000269"
     CDS             11590..14406
                     /gene="uvrA"
                     /locus_tag="MBZ71_000269"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBT4428252.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14669..15934
                     /locus_tag="MBZ71_000270"
     CDS             14669..15934
                     /locus_tag="MBZ71_000270"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBT4428253.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15938..16669
                     /locus_tag="MBZ71_000271"
     CDS             15938..16669
                     /locus_tag="MBZ71_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBT4428254.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            16804..17688
                     /gene="rapZ"
                     /locus_tag="MBZ71_000272"
     CDS             16804..17688
                     /gene="rapZ"
                     /locus_tag="MBZ71_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBT4428255.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            17685..18683
                     /locus_tag="MBZ71_000273"
     CDS             17685..18683
                     /locus_tag="MBZ71_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBT4428256.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            18717..19652
                     /gene="whiA"
                     /locus_tag="MBZ71_000274"
     CDS             18717..19652
                     /gene="whiA"
                     /locus_tag="MBZ71_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBT4428257.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20220..20876
                     /locus_tag="MBZ71_000275"
     CDS             20220..20876
                     /locus_tag="MBZ71_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428258.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            21055..21620
                     /locus_tag="MBZ71_000276"
                     /pseudo
     CDS             21055..21620
                     /locus_tag="MBZ71_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22319..22840)
                     /locus_tag="MBZ71_000277"
     CDS             complement(22319..22840)
                     /locus_tag="MBZ71_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBT4428259.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            23038..26718
                     /gene="nifJ"
                     /locus_tag="MBZ71_000278"
     CDS             23038..26718
                     /gene="nifJ"
                     /locus_tag="MBZ71_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBT4428260.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            26907..27794
                     /locus_tag="MBZ71_000279"
     CDS             26907..27794
                     /locus_tag="MBZ71_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBT4428261.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            27791..28960
                     /locus_tag="MBZ71_000280"
     CDS             27791..28960
                     /locus_tag="MBZ71_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428262.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            28984..30339
                     /locus_tag="MBZ71_000281"
     CDS             28984..30339
                     /locus_tag="MBZ71_000281"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBT4428263.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(30411..32216)
                     /gene="glmS"
                     /locus_tag="MBZ71_000282"
     CDS             complement(30411..32216)
                     /gene="glmS"
                     /locus_tag="MBZ71_000282"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBT4428264.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            32626..32916
                     /locus_tag="MBZ71_000283"
     CDS             32626..32916
                     /locus_tag="MBZ71_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428265.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            32929..33954
                     /gene="sppA"
                     /locus_tag="MBZ71_000284"
     CDS             32929..33954
                     /gene="sppA"
                     /locus_tag="MBZ71_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBT4428266.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            33957..34616
                     /locus_tag="MBZ71_000285"
     CDS             33957..34616
                     /locus_tag="MBZ71_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBT4428267.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            34773..35705
                     /locus_tag="MBZ71_000286"
     CDS             34773..35705
                     /locus_tag="MBZ71_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428268.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            35859..37148
                     /locus_tag="MBZ71_000287"
     CDS             35859..37148
                     /locus_tag="MBZ71_000287"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBT4428269.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            37395..38645
                     /gene="clpX"
                     /locus_tag="MBZ71_000288"
     CDS             37395..38645
                     /gene="clpX"
                     /locus_tag="MBZ71_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBT4428270.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            38728..39315
                     /locus_tag="MBZ71_000289"
     CDS             38728..39315
                     /locus_tag="MBZ71_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBT4428271.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(39389..39544)
                     /locus_tag="MBZ71_000290"
     CDS             complement(39389..39544)
                     /locus_tag="MBZ71_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428272.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            39680..41008
                     /locus_tag="MBZ71_000291"
     CDS             39680..41008
                     /locus_tag="MBZ71_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428273.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        13533 a         7109 c         8960 g        11542 t
ORIGIN      
        1 ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg taaaaaaatc
       61 atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg
      121 cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga
      181 aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta
      241 ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt
      301 tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac
      361 gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa
      421 tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt
      481 gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg cagccagtag
      541 atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt catcctacag
      601 cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct gctaaacaac
      661 ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta
      721 ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg aaaaactatg
      781 gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg aaaaaaattg
      841 gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa gcagaaaatt
      901 ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc
      961 gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga
     1021 tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg
     1081 atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac
     1141 gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca
     1201 tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt tatcttggag
     1261 atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta cgagcaaatg
     1321 aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt
     1381 tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa
     1441 aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg
     1501 ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg aacaatgtgt
     1561 ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc gaaacttgaa
     1621 aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa ctggatttac
     1681 atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct
     1741 tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga atatatgagt
     1801 gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca
     1861 tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg tctgagggag
     1921 tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc
     1981 tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg
     2041 tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt
     2101 ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat
     2161 ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac cgccgactga
     2221 ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata
     2281 tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag
     2341 aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc
     2401 ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg acagaaggga
     2461 tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt tatgtcagtg
     2521 gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt
     2581 tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata
     2641 ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc
     2701 aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta
     2761 gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta
     2821 aacttgctga ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct
     2881 atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat
     2941 gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg
     3001 ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg
     3061 aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagataaat
     3121 acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc
     3181 aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc
     3241 tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg
     3301 tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc
     3361 ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga
     3421 ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac aaattacatg
     3481 atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga tataaaactt
     3541 acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg
     3601 tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc
     3661 agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg
     3721 ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca
     3781 tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat
     3841 ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt
     3901 cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa
     3961 agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc
     4021 tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct
     4081 tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt
     4141 gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta
     4201 catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt
     4261 acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac
     4321 acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac
     4381 attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc
     4441 agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg aagttatttt
     4501 agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa
     4561 agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg
     4621 tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt
     4681 aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc
     4741 aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca
     4801 tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt
     4861 gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga
     4921 taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat
     4981 cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg tgaaagatgc
     5041 agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt
     5101 agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga
     5161 agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg ttgaagcaaa
     5221 caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac
     5281 tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag aagcagctca
     5341 aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata
     5401 atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa
     5461 aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg
     5521 aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt
     5581 cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc
     5641 gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt
     5701 atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg accaatacgg
     5761 gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt
     5821 cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg
     5881 aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac
     5941 ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg
     6001 ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac aaccaactac
     6061 ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc cacttggacg
     6121 agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa tcaaagaacc
     6181 ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt
     6241 accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga
     6301 aaacggcgga ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa gtgatatctt
     6361 tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt
     6421 gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg
     6481 aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg
     6541 tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca
     6601 acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca
     6661 agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt
     6721 tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta aaaacgctaa
     6781 gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctggtttt tttgtagttg
     6841 tcatgtttgt accaacaaaa aagccagctt tagaataaga taatcatcat agtttctgaa
     6901 ggtggtatcg actatgtact tgatatcgtc aatgaaccga tggtactttc ttatgcgatg
     6961 gaacaggtag agcgattgct tgtttgtgca ggagaacaag cgatgcggct ggtacagtta
     7021 agtgaaaaaa caagaaacta aaaatgcccg gtcgtcatga agcgaccggg catttttggt
     7081 ttttattaaa tatttaatac tttattcaag aaatcttttg tccgttcatt ttgcggattt
     7141 tcaaagattt gttgaggcgt tccgtcttct aaaaagtttc cgtcgtcaat gaacatgaca
     7201 cgattggcca cttctttagc gaagcccatt tcgtgcgtaa cgataaccat cgtcatacct
     7261 tgttctgcta actttttcat gacgttcaag acatcaccga ccatttcggg gtcgagtgca
     7321 gatgtaggct catcaaaaag catgatgtca gggttcatcg ctagagcacg agcaatagcc
     7381 acacgctgct tttgtccacc ggaaagcatt tcaggataca tatctttttt atcttctaga
     7441 ccaactgtct gcagcaattt gattgctttt ttctctgctt cttctctaga ttctttcttc
     7501 aggtccatcg gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa taggttaaag
     7561 tgctggaata ccattccgat atgttgtctc accaagttga tatctgtgct tttatccatc
     7621 aagtgggcgc cgtcaataat aatttcaccg tcagaaggtt cttctaaacg attcaagcaa
     7681 cgcaaaaatg tactttttcc tgaacctgaa gggccaatga tacagacgac atctccttcg
     7741 ttgatggaca cattgatgtc gtttaatact gtgttatcgc cgtatttttt gactaaatgc
     7801 tcaactaaga ttttttctgc catcttactt caccttcttt tctaaaactt ttgctaattt
     7861 tgtcaatgct gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa catacgtact
     7921 ttgtgttgta cgtgcaacaa tgatcttacc agtttgcagc aattcgacta caccgattgc
     7981 ggaaatgatc gttgtgtctt ttaatgagat aacgaactga ttgacgaatg atgggatcat
     8041 gatgcgtatc gcttgcggca aaatgatttt ttgcattgtc cgattataag aaagacctaa
     8101 acttctggaa gcttccattt gtccgacagg aacggcgttg atcccgcctc gaacaatctc
     8161 tgcgatataa gcacttgcgt taagagtcaa ggtgataaca ccagcaagga agtcaggaat
     8221 cgtaaatcca acgataccag gcaacccaaa gaaaataaag aacgcaagaa ccatcattgg
     8281 aattcctcgg atgatatcga cataaatact agcaattgtg cgaagagcgc gtattggtgc
     8341 tacactgaat aatccaaaaa tcacaccgat tactatagcc aaagcaaagg agatcaaagt
     8401 taggagaatt gttctccaaa gtcctttcaa caatgcttga tagttgtttt ggataagtcc
     8461 aagaaatgtt gattcatcag cagtggaaga ctcactggaa gtttctgaac catccgctac
     8521 atattcttta ataatcttat cgtattcgcc tgttcttttt aattctttca atccttcatt
     8581 gaacatatta agaagctcag gattttgtcc ttttttaacg gcaaatgcat attttccgcc
     8641 agattctttt ttgatcggtg tttccaattc ttgtccttgt ttgatcgcat aaccgattac
     8701 cggataatca tccatcattg catcgattgc gccaactttt aacccgtcgt acaattgatc
     8761 agcagcatca aaatatttga tagaatagcc atattcagat tcatggtcgc ttaaaaattc
     8821 agcactttcg gtaccaactt ttgctccgac tgtttttcct ttcaaatctt cataagactt
     8881 gatcgaagta tctccttttt tgacagctaa ttggatcccg ctatcaaagt aagaatcaga
     8941 aaaatcatag cttttctttc gttcatctgt tacagtcatt ccggcaatca ttccgtctgc
     9001 ttgtcctgat tccaaagctt gcaaagcagc agagaaacca cggtagtcca ttcggatgtt
     9061 aaatccttgc agtttggcga tgttttccat caattttaca tcgatgcctt cgtactcgcc
     9121 ctttgcgttt tgaaattcaa atggagcaaa agcagagtca ctggcaatta cgtatacgtc
     9181 ttttttgggc tctatttttt gcattgtctc gcttgtgctt gactctgccg ttgtagtgtt
     9241 gtcagcttgt gcaacatgtc caaacgcaag tgtcacaagt cctgtcatta agaaaacaaa
     9301 tgagtgaaat aaatgctttc ttttcataat caacctccta aatttccaat attttattat
     9361 tataggcgaa aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga aaaaaatgcg
     9421 agtttctaac attttatgtt ataaaacgta acatgcataa taagataaaa aagtggcgaa
     9481 agtatgttcg gtgtggtata attttttagt aaggaatgcc tttgctttcc ttttcttttt
     9541 attggtcgta aaatgaaaag gaatgaatga aaaggaggac gaagaatgat tgagagagat
     9601 acctcccgac actttgagct tgtctcgaaa taccaaccgg ctggtgatca gcctgaagcg
     9661 attaaccaat tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt attaggtgcg
     9721 actggaacag ggaaaacata tacgatttcc aatcttattg agaaagtgaa taaaccaaca
     9781 ctaattattg cccataacaa aacattggct ggtcagctgt acggggaatt caaggaattt
     9841 ttccctaaca atgctgttga atattttgtt tcttattatg attattatca gcctgaagct
     9901 tatgtgcctt caagcgatac gtatatcgaa aaagattcaa gtgtaaatga tgagatcgac
     9961 aaattacgac attcggctac tagttcatta ttagaaagaa atgatgtgat cgtcattgca
    10021 tccgtttcat gtatttttgg attaggttcg ccttttgagt accaaaaaca agtagtttcg
    10081 attcgacagg gagcagaact agatcgcaat cagttgattc gtgacttagt cagtatccaa
    10141 ttcgaaagaa atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg tgatgtcgtg
    10201 gagatttttc cggcttcaag agatgaacgt gccttacgtg tggagttttt tggagatgag
    10261 gtcgaacgta tccgagaagt caatgcactg acaggtgaag ttttaggaga aacggaacac
    10321 gtggcaatct tcccggcgac tcactttgtg acgaatgatg aacatatgga gcatgccgta
    10381 gcgaatatca aagcggaatt agaacagcgt ttaactgtct tgcgaaatga aaacaaactt
    10441 ctggaagctc aacgtttaga acagcgaacc aattacgata ttgaaatgat gttagaaatg
    10501 ggctacacgt cgggaattga aaattattct cggcatatgg acggacgtaa agaaggtgaa
    10561 ccgccttata cgctattgga tttctttcct gaagattttt tgatcgtggc ggacgaatcg
    10621 cacgtaacca tgcctcaagt cagaggaatg tataatggtg accgagcgag aaaacagatg
    10681 ctagtagatt acggtttccg tttgccatct gctttagaca atcggccact tcggttagaa
    10741 gagtttgaaa aacatgtcaa tcagatcatt tatgtctctg cgacgccagg accatatgaa
    10801 catgaacaaa cagataccgt tatccaacaa atcatccgtc cgacagggct gcttgaccca
    10861 gtgatcgaag ttcgtccaat catgggacaa atcgatgatc tagtaggcga aataaatgaa
    10921 cgagtcgaaa aagaccagcg ggtgtttgta acgacattga cgaaaaaaat ggcagaagac
    10981 ctcacagatt atttcaaaga attagggatc aaagtcaaat atttgcatag tgatatcaaa
    11041 acgctggaac gaacagaaat cattcgagat cttcgattag gtgaatttga tgtgttagtt
    11101 ggaatcaatt tgcttcgtga aggattagac gtacctgaag tttcccttgt tgctatcttg
    11161 gatgcggata aagaaggctt tttacgtagt gaacgttcct tagtccaaac gattggacga
    11221 gctgctcgga atgaagaagg taaagtaatc atgtacgcag acaaagtaac agattctatg
    11281 cgtctagcga tggatgaaac atcaagacgc cgtacgattc agcagaagta caatgaagaa
    11341 catgggatcg ttccaaaaac gatcattaaa gaaatccgag atttgatttc tattacgaaa
    11401 gaatcggaag atgatacaaa agaagcggta caagtctcgt atgaagaaat gacgaaagaa
    11461 gaaaaagata cgttattgat gaaattagaa aaagaaatga aaaatgcggc gaaagcattg
    11521 gattttgaaa cagccgctaa tgtgcgagat atgattttag aattaaaagc ttcaaagtag
    11581 gaggacaaca tggctaacga taaaattacg attcacggtg cacgtgccca taacttgaaa
    11641 aatatagatg taacgattcc acgagataaa ttcgtggtcg ttacaggttt gtccggttct
    11701 ggaaaaagtt ccctagcatt tgatacgctg tacgctgaag ggcagcggag atacgtagag
    11761 agcctttctg cttatgctcg tcagttttta ggacaaatgg ataagccgga cgtagatagc
    11821 atcgacggat tgagtccggc gatttctatt gatcagaaaa caacaagtaa aaatcctcgg
    11881 tcgacagtag gtactgtgac ggaaatcaat gactatttac gcttgttgtt tgcacgtgta
    11941 ggacatccga tttgtcccaa cgatcatatc gagattacca gtcaatcagt agatcaaatg
    12001 gcagatcaag taatggcttt gcctgagcgc acaaagatcc aagtgttggc accgatcgtg
    12061 gtaaagaaaa aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga gggatacgtc
    12121 cgcgttcgag tcgatggtga gatctatgat ttaagtgaag tgcctgagct ggagaaaaac
    12181 aaaaaacatg atatcgcgat cgtcatcgac cggatcattg tgaaagaagg gattcgttct
    12241 cgtctatttg attctttaga ggcggctctg cgattggcag acggctatgc tctggttgac
    12301 gtgatcggtg aagaagaaat gttgtttagc gaacactatg cctgtcctta ttgcggattt
    12361 actgttggcg aactagaacc aagactgttc tcattcaatg cgccttttgg agcttgtcca
    12421 gactgtgatg gtttaggtgt caaattagaa gtcgatcaag atttagtgat tccagataga
    12481 acgaagacac ttagtcaagg tgctatcgtt ccatggaatc caatcagctc tcagtattat
    12541 cctcaaatgt tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac accattcgaa
    12601 gaattaccgg aagagcatca ggatatcatc ttgaatggga cagatgatct tttccatttc
    12661 cattatgaaa atgattttgg tggcgttcgc gatgtagaaa cgactttcga aggggtattg
    12721 aagaatatcg agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga gcaaatgcgt
    12781 ctttatatga cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa tccccaagct
    12841 ctatctgttc aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc tattcaaaaa
    12901 gcagtagatt tcttctcaaa cttaacgtta tctgaacaag agaaagtaat tgccaaaccg
    12961 attttaaaag aagtaaatga cagactttca ttcttggaaa atgtgggact agactatcta
    13021 actttgagtc gcgcttccgg aacattatct gggggagaag cgcagcgaat cagattggct
    13081 acacaaattg gatcgaatct ttctggagtt ttgtatattt tagatgaacc atcgatcgga
    13141 ttgcatcaac gcgacaacga tcgactgatt ggctcattaa agaaaatgcg tgatcttggc
    13201 aacaccctcg ttgtagtcga acatgatgaa gatacgatgt gggcagcaga ttatctgatt
    13261 gacgtcggac ctggggccgg agaacaaggc ggacaaatcg tggctgcagg cactcctgaa
    13321 gaagtagcta atgatgaaaa ttctttgaca gggaaatatc tttcagggaa aaaatcaatc
    13381 cctgtaccaa aagaacgccg taaaggtaat gggaaagcaa tcaagatcac tggtgcttca
    13441 gaaaacaatc tgaaaaatat tagtgttgaa tttccattag gagaatttgt agcagtcaca
    13501 ggtgtgtctg gttctggaaa gagtacattg gtcaatagta tcttaaagaa atcgttagcg
    13561 caaaaattaa ataagaattc tgctaagcca ggtaaattca agacaatttc cggctacgaa
    13621 agtatcgaaa agatcatcga tatcgatcaa agcccaatcg gccggacgcc gagaagtaat
    13681 ccagcgactt atacaagtgt atttgatgat atccgtgggt tatttgctca aacgaacgag
    13741 gcaaaaatgc ggggttataa gaaagggcgt tttagtttca acgtaaaagg cggtcgttgt
    13801 gaagcttgtc gcggggatgg aattattaag atcgaaatgc actttttgcc tgatgtctat
    13861 gttccttgtg aagtatgtca tggcaaacga tataactctg aaacattaga agtgcattac
    13921 aaaggaaaaa gcattgctga tattttggaa atgacagtag aagatgctgt agaattcttc
    13981 aagcacattc caaagattca tcgcaaactg caaacgattg ttgatgttgg cttaggttat
    14041 gtgacgatgg ggcaaccagc aacgacattg tccggtggtg aggcacaacg gatgaaactt
    14101 gccagtgaat tgcacaaaat ctctaatgga aagaatttct atatactaga tgaaccaacg
    14161 acaggacttc atagcgatga catcgcccgc ttgttgcatg tattacaaag attagtagat
    14221 gctggtaaca cagttttagt gattgaacac aatctagatg taatcaaaac agcagattat
    14281 atcattgatt taggaccaga aggtggagaa ggtggaggaa cgatccttgc gactggaaca
    14341 ccagaagaaa tcattaacgt aaaagaaagt tatacaggtc actatttgaa aaaaataatg
    14401 gtataaagag gatagatagc tgagatagtt aaaaaaacat aaaaaaatga atttttgctt
    14461 gttttattaa tatcagtgtt ctagaatcat tacatggtag ttctttctac actgattggt
    14521 atagacaaaa acttcctata agttcaatcc gttcaaaaaa gaattgagca atcggaggag
    14581 tttcctttga ttgctttttt ctttatggta tgatattcct tgtgtagttg gaaaagaaaa
    14641 acaagagatt atgagtaagt gagagataat gatgaacagt attgaaaatg aagaatttat
    14701 ccctatatta ttaggaagcg acatgaacgt ttatggtatg gcacgttctt ttaatgaagc
    14761 atacggcaaa atctgccaag catatgcttc ggatcagtta gcccctaccc gctacagtaa
    14821 aattgtcaat gtagaagtta ttccaggatt tgataaagac cctgtgttta tcgagacgat
    14881 gcttcgtttg gcaaaagaaa gatattctga taagagtaaa aaatatcttt tgattgcttg
    14941 tggtgatgga tatgcagaat tgatctccca acataaacaa gagctgtcag aatattttat
    15001 ctgtccatat attgattatt ctttatttga aagactgatc aataaagtaa gcttttatga
    15061 agtttgtgaa gaatatgatc ttccgtatcc taaaacattg atcgtcagag aagaaatgtt
    15121 agtaaatgga catttggaac aggaattgcc ttttgaattc ccagttgctt tgaaacctgc
    15181 aaacagtgtg gagtatcttt ctgtccaatt tgaaggaaga aaaaaagctt ttattttaga
    15241 gactagagaa gagttcgatt taattttagg ccggatatac gaagccggtt ataaaagtga
    15301 aatgattgta caagatttta tcccgggtga tgatagtaat atgcgtgtgc taaatgcgta
    15361 tgtagatgaa gatcatcaag ttcggatgat gtgtcttggt catccactgt tagaagatcc
    15421 tacaccagca tcgattggta attacgttgt gatcatgcca gattataacg aaaaaatcta
    15481 tcaaaccatt aaggcatttt tggaaaaaat cgaatataca ggctttgcca atttcgatat
    15541 gaagtatgat cctagagatg gagaatataa attatttgaa atcaatttga gacaaggtcg
    15601 tagcagtttc tttgtgacat tgaacggctt gaatctggct cgtttcgtca cagaagatcg
    15661 agtgtttaac aaaccctttg ttgaaacgac ctatggaacg aatcaatcag ataaagcaag
    15721 attatggatg ggtgtgccca aaaaaatctt tttagaatat gctcgggaaa atgaagacaa
    15781 aaaactagct gagcaaatga tcaaagaaaa tcgttacggt actactgtgt tttatgaaaa
    15841 agaccggtca atcaagcgct ggttgttgat gaaatatatg ttccataact atattccaag
    15901 attcaaaaag tattttcatg tgaaagaagg ctagttcatg gagaattttt tcagtatttt
    15961 aggcggaatg ggcacgatgg cgacagaaag ttttgttcgc ttgatcaacc accgaacgaa
    16021 agctacaaaa gatcaagaat atttgaatta tgtcttattc aatcatgcaa cagttcctga
    16081 tcgtacagct tatattttag atcgatcaga agaaaatcca atgccgtttc tgctggatga
    16141 tattgagaaa caaaatttat tgcagccgaa cttcattgtg ttgacttgta acaccgcaca
    16201 ctactttttt gaagaattac aagcggcaac ggatattcct atccttcata tgccaagaga
    16261 agcagcaaat gaacttgtcc gtcagcatac gacagggaga gtagcgattc taggaacaga
    16321 aggaagtatg aaagctggaa tctatgaacg cgaagtcaaa aatcttggat tcgaaacagt
    16381 gattcctgat actgttcttc aagaaaaaat caattatctg atctatcatg agatcaagga
    16441 atcggattat ttgaatcaag agctatatta cgaaatctta gaagaagcgg tagagcgcct
    16501 aaactgtgaa aaagttatct taggttgcac agaattgtca ttgatgcatg aatttgctga
    16561 agataaccat tatccagtga tcgatgccca atctatttta gcagatcgga cgattgaacg
    16621 agcgctagct gaaagaagca aggcattgga tacagcatcg gaaaagtaaa aaaattggat
    16681 ttggggcaaa tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga aaaagaaaac
    16741 tggtaatgat acgcccggaa tgatataatt ttactatgca caatgtgaga ggagttcgta
    16801 attatgacag ataatttgca attagtcatc ataacaggta tgagcggtgc agggaaaaca
    16861 gtagctatcc aaagttttga agatatgggc tatttttgta tcgataatat gccgcctcga
    16921 ttgattccaa aattctggga attaatcaaa gaatcaggca aagtcacaaa aatggcttta
    16981 ggagtggatt tacgttctcg ttccttcttt gaagaaatcc aaaacatgct gatcgaaatc
    17041 gaaaatacga acttcatcga cacttctatc ttatttttag atgcttcaga cgaagaacta
    17101 gtggctcgct acaaagaaac aagacgagca catccaatgg cgatggacgg tttgatcact
    17161 gaagggatac gtaaagaacg ggcgatacta gacgacttga aagcccaagc ttccgtgatc
    17221 attgatacga cgacattgac accacgacaa ttacgtgaaa aaatcaatga gtcattcaaa
    17281 tcaaagggag accaaggatt tcggatcgaa tttgtatcat ttggttttaa atacgggttg
    17341 ccgatcgatt cggatattgt gatggatgtg cgttttttgc ccaaccctca ttatatcccg
    17401 gaactaagac cattgaccgg aaaagatcag ccagtctatg attatgtgat gtcctttgat
    17461 gaaacgcaat cattctacca taaatttttg gatcttctgg aaactgtatt gcctggatac
    17521 attaaagaag gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg tcaacaccgt
    17581 tctgtggcat tgactgaacg tttggcaaat gctttaggga aaaactataa agtcaatgtg
    17641 acacatcggg acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa atgaaaacct
    17701 accggataag aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg ccagttatcc
    17761 tgaaaagttt gcgaaatcaa ggagcagata tcacagcagt agtgactgtt gcagatgatg
    17821 ggggaagcag cggtgcaatc agagaatcga tcgctatggc tcctcctggt gacttgagaa
    17881 atgtcttagt ggcgttatcg gatatgccac aattctatga agatattttc caataccgtt
    17941 tcaaaaagga agatcagttt ttagccaatc atacgatcgg taatctgatc attgccgcag
    18001 tttccgagat gcggggaagc acttatgagg cgattcaatt attagcaaaa atgatgcatg
    18061 tagatggaca tgtttatcct tcttcagaaa ctcctttgac cttgcatgct gtctttaaag
    18121 acggaacgac tgcagtagga gaatctaaaa tcgcgatcga ccgtaaaacc attgaccgtg
    18181 tgtatgtctg caacacaaat gataaaagtg aagccaaagc ggctcgaaaa gtcgtttcag
    18241 caattatgga cgccgacatg gtcgttttgg gacccggaag tttattcacc agtatccttc
    18301 caaatttagt gataccagaa atcggcgaag caatgctcaa gacaactgct gaaaccgtct
    18361 atatctgtaa tatcatgacc caaaaagggg aaacggaaca tttcacagat gccgatcata
    18421 tacgtgtttt acacaagcat ttgaatcagc cttttatcga tacagtattg gtcaatacgg
    18481 aaaaagtgcc tgaagattat atggacccag agatctatga tgagtatcta gtacaagtcg
    18541 cacacgactt caacggattg agaaatgaag gatgtcgagt gatttccacc gactttttgg
    18601 aattgaaaga tggtggtgtc ttccacgatg gagacaaagt agtagaagaa ttgttccgtt
    18661 tagttttcgg ttcaaaatat taagcaattt attgttggag aaaggaggag aataggatgt
    18721 catttgctgc agatgtgaag aaagaactaa ctggtttgga agttcatcga gaacatgcga
    18781 aagcggaatt agctgctttg ctgcgaatga acggctcttt aggtattgtg aaccagcagt
    18841 ttattttgaa cgtccaaaca gaaaatgctg caattgccag acgtatctat tctcttttga
    18901 aagatcatta tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa ttaaaaaaga
    18961 acaatgtcta tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg gatcttgata
    19021 tcatggatgg tatgatgttc aatggacacg tttcggacga gattatggga aattcgcaaa
    19081 aaatgcgttc ttacttacgt ggtgctttta tggcctcagg ttcagtcaat aatccggaaa
    19141 cgagccgtta tcatttggaa atattttcta tttatgaaca gcataaccaa gacatttgcg
    19201 atatgttaaa ttattacggt ctacatgctc gaacgcttga acggcgcaat ggctatattt
    19261 cttatttaaa aggagcagaa aaaattgctg atttcttaac attgattggt gcaaccaact
    19321 caatgctccg atttgaagat gttcgaatcg tacgggatat gagaaattcg gtcaatcgtc
    19381 tagtcaattg tgagacagca aatctgaaca agacgattga tgcggcttct aaacaaatcg
    19441 aaaacatcga attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa aaacttcagg
    19501 aaatcgcaga acttcggctt caacatccag aggtcagctt gaaagaatta ggggaaatga
    19561 ttccctcagg tgcgatatca aaatcgggaa ttaatcaccg aattcgcaaa ataaatgaat
    19621 ttgcggaaaa actgcgtgaa gaagcagttt aatccaaaga aaaaactgat acatgaagca
    19681 ggagatgcga atgtatcagt tttttttaaa agttatactt gcttttcttc cgtcaaacta
    19741 taattccatc tttactggag taacataatt tggaaaggct tctattgttt ttaatacaat
    19801 cgtataaaaa gcggggagac aactgaatga agattttagt tttgtacata aatttttgct
    19861 taatatagaa gtcctgatat agataagatc aagtatttat ctataactgg cctgattaat
    19921 taaaaaactg gaaggaactg ctcgatcatc cttgagaact gctcttctta cttctggtca
    19981 agattctcgt ggttggtgga taaggatggt tgataagttc attggattag ctagtcgaaa
    20041 acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt tattcagtga
    20101 taatcataag ttcataaata gtaattgaga tgtatggttg gaatacgtta tacctgaact
    20161 tgaactacca agatttggtt tagaatttga gaatcgatgt ttataaaggg agggcaaaaa
    20221 tgcgtttttt tacaactttt actatcatta tgattgcagt actttttatt tttctagata
    20281 tagcaaagcg aaatacagca tttttattat atcgggtttt acttcgagca ggtctcataa
    20341 catttatatc gatcgttgga ttttttctat tcacagttat tgtatttatt tggagaactc
    20401 cagcaccacc attgccagag attacatatg gagaatttcc ttttagactt gagtatgaat
    20461 tgaatgaaga attacatgtg atagaagaca cgttaatagt tgaatttgat ggatttggaa
    20521 tgaacgaagg catagggaga tatcgtagat ggacaagtcg tttagccagc ggagaagatt
    20581 tagtgttgct gttagaagtt agtgataata agcaaatatt ttattttcca ggacctgcta
    20641 attattacat gggagatagg ttgaatggtt ataatcatac ttttcctagt gcttctttca
    20701 ttgaaaggga aagaggcatt actagaaggg atattttaca tgacaaagaa ttattagaac
    20761 aatttggtcc gcttgatcaa aatacaataa atgaagaaga attgttaaat caatataata
    20821 taaggctagt gaattgggaa atatctgaac cgattgttaa caattttggt gattagaaaa
    20881 caattacgat aaatagaaac aagccaaatc atcagcaagt aaaagctgat gatttggcct
    20941 ttcttatacg agtagtagga attcatagcg aatattatta cgatcagtag atattttgat
    21001 attaaaggag tattacgaac tagtatgtga gaatgtagag gtgatttgcg aataatgaga
    21061 aatttatcaa atagaaacaa aatattaatc attattgttg ttatagctgt gtttcatctg
    21121 ggaacgaatg cggttttaag tagaataata ttgggtccta agccaccaag acctgcgatt
    21181 acccgagggg agtttgattt tagattagaa tatgaagtag atggggaaag gattgtgata
    21241 gaagatacaa ttgttgcatt atttgatgga ttttctgcag atgcaggtag catggcatgg
    21301 tatcgcacgt ggcgtttgca tttggcgagt gacaagaggg cacaacaatt attgttacat
    21361 gagttagatg atggtcgaaa aatatattat cattttggag gagctggatt ttatatgggg
    21421 aagttagtag cagtgtaact gtaacaagga aagatttgcc aggtcttggc attcgaaact
    21481 ccatatttta tatcgagcgt gggagagata catctattgg gaacaggaaa gggttaactc
    21541 ttgaagagtt atataatgac tttggaatcc gtcttgttag ttgggaacac aatccaccaa
    21601 tagaaaatcg ctttgaataa tcgaaatcta ttaatccatt tcatcagcca gtgaatgttg
    21661 taatggaaca atttggttta gtgttcaaaa ccattgaaat actaggctgt gatcagcaaa
    21721 aatgtatctg aataaaaaag agagcaacta ttaaatgaga ggagttagga gttgttagct
    21781 ttatacgaat caaagtggca aatacagatg cgtatgcatt tgtgtttgcc atcttgcgtt
    21841 tggtagacga gataggtata agctaaagaa acaacttgtt taaaataaac gacctagctc
    21901 aacaaacgac catctaatca actaacagca tacataaaag caactatttt tgaagtagaa
    21961 ttgaaatcgg gataacttaa aaagtgattg ggatactagt gtgaatcacc gtacataaat
    22021 attactcgtt aagggaattt gagaaatttt gtatgaaaat ttaagaatta gttagtatgc
    22081 gcaagtcaaa aatatgaata aattatttgg tggagataat ttgctcagct tgtcataaga
    22141 aatgagataa tctggcagat gcaagctttt ggacaccatt tattcggaat aaggaggctg
    22201 ggacagaagc gtttaactcc aagaaataag aaggagttgc ttctgcttcc gccgtttata
    22261 ggtgtttaga gcgtgagaca aaagtgttct ttacttttgt cctacgctcc ttattttttt
    22321 attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca aagatttctt
    22381 tacccaagat gacagtcgga acaaatcgaa tatgtgcttg ctcagcttcg ttgatgacag
    22441 cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact gcaaaatccg
    22501 ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt tgtttgatct
    22561 ctttgatcgc tttttgacca tcgctgattg tgagataccg gtgcatcacg tttcctcgct
    22621 gcaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc ccgctttgga
    22681 ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac ggacaagata
    22741 ggttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg attccttttt
    22801 gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac ctctttcgct
    22861 tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt ctaaaaatta
    22921 gatgaccaat tcaaatagac atatgtgaaa tatttttata aataaaagaa aaaatggaat
    22981 gttttacgtt ataatggaac agataataag agagaatatg aacaaggatg atgaatcatg
    23041 cgaaaaatga aaacaatgga tggcaatacg gcagccgcat atatttctta tgcatttacc
    23101 gagattgctg caatatatcc aataacaccc agttcgacca tggctgaatt agtagatcaa
    23161 tggtcagcag caggaaaaga aaatgtattc ggtcaaccag taaaagtagt agaaatgcaa
    23221 tccgaagcag gagcagctgg tgtggtccat ggatcattga aaactggtgc actgacaact
    23281 acatataccg cttctcaagg gctgcttttg atgataccga atatgtacaa gattgctggg
    23341 gaactactgc caagtgtttt ccatgttgct agtcgggcag tgacgacaaa tgcgttgaat
    23401 attttcggag atcacggaga cgtgatggct gcaagacaaa caggatttgc tatgttagct
    23461 gaaagcagtg tacaagaagt gatggattta gcacctgttg cccatcttgc ttctattgaa
    23521 ggagaagtac cttttttgaa tttctttgat ggattccgta cgagtcacga gattcaaaaa
    23581 atcgaagttt tggaatatga agaattagct cctctgatca atcaggagaa attagcttca
    23641 tttcgtaaac gagggatgaa tcccaatcat ccgactgtta gtggtacgaa ccagaatcca
    23701 gacatccatt ttcaacaacg ggaaacgatc aacccctatt atgataaatt acctgaaatc
    23761 gttcaaaaat atatgaaaga gatcaaccga ttacgaggaa cttcttatga cttggtgact
    23821 tattacggag cagacgatgc ggaagaagtc attgtttcta tgggatcagt tgcacagacg
    23881 attgaacaaa cgattgatta cttgaatgca aacggacgga aaacaggttt tttgaatatc
    23941 catctttatc gtccttttcc agtggaaaca ttcgtgcaaa agttgcctca tacagtaaaa
    24001 acaattgctg ttcttgatcg aagtaaagaa ccaggggcag gtggagaacc tctcttatta
    24061 gacgttcaaa gtgcgttata tgattctgaa cttcgcccgg ctgttattgg tggtcgctac
    24121 ggattggggt ctaaagatgt gactcccgac caaatctcag cagtttttga tgaattgaaa
    24181 aaagacccaa gcatacgcaa aaaacggttt accataggga tcgtggatga tgtgacttat
    24241 caatctttgg aaccaaagga aagtctggat ttgacagaac cgcaaacatt ccaggccaag
    24301 ttttggggat ttggatctga cggaacagtt ggagcaaata aatcagctat caagattatt
    24361 ggagatcaca ccgataaata tgcgcaaggc tatttttatt atgattcgaa aaaatctggc
    24421 ggtctgactg tctctcatct tcgttttgga gatacgccga tacgttcagc gtatcttgtc
    24481 gagcatgcgg accttgttgc ctgtcacaca ccagcttacc tgcatagcta tgacttggtc
    24541 aaaggattga agccaggtgg cattttttta ttgaatactc tttggagcga cgaacaatta
    24601 gaaacgcatc tgcccttaaa attaaaacgt tacttggctg aaaataacat tagattctat
    24661 acgatcaatg ccatgagatt ggcccaagaa gtcggtttgg gacgccggat caatacggca
    24721 atggaaacgg ctttttttaa attagctgac atcattcctt ttgatgaggt gcttccgctg
    24781 ctaaaagaag aagccctgaa aagctatgga cataaatcaa tggctatcgt agaaaaaaac
    24841 atacaagcaa tcgatcggac gaccgaactg cttcatcaag tcgcgatccc agaccattgg
    24901 agaacgttgg agatgcctga aaaaatcaat aaaaggaaca ctggttatat acaagaaata
    24961 cttgaaccag tgaatgcaca ggaaggaaat caactctctg ttggtacact tgtagcaaat
    25021 ggaatgacaa ctggcgaaat gcctttagga acggcttcta tggaaaagcg gggcgtcgct
    25081 ttagaagtac ctgagtggat tagtgatcgc tgtacgatgt gtaatgaatg tgcctttgtt
    25141 tgtcctcatg ctgccatccg tccattttta gcagatgaag aggaaatgga agaagcgcca
    25201 gaaggattta tcgtgagaga aatgcgaggc gcagacggcg taaaataccg tatccaagtt
    25261 tcagtggaag actgtacagg ttgtggtctg tgtgtagagg cttgtcccgc aaaagaaaaa
    25321 gctttagtga tgaagcctta tgaagaacag aaagaacaag ctgtgaattg ggcttttgca
    25381 atgacattaa gacaaaaagc aaatccagtc aaaggaaaga atactgtctt gtctacacaa
    25441 ttcaatcaac cgcttcttga attctcgggc gcttgctcag gctgtggcga aacaccttat
    25501 gtaaaattat tgacacaaat gttcggtgat cgaatgatga tcgcgaatgc gacaggctgt
    25561 tcttctattt ggggcggtgc ttctcctgct tctccttata cgaccaatga atgtggtcaa
    25621 gggcctgcat ggtcaaattc tttattggaa gataatgctg aatttggtta tggtatgctt
    25681 ctagcggcac agactcgtcg cgaggctttg gcaagttcaa tcaaagaagt attgccaaaa
    25741 gtatcagaag aactacgaga attgatggaa gactggttgg tacacatgtt tgagagcgaa
    25801 ggaacgcagc aaagagcagc aaaactgcaa gctgcgatcg aaagcgaacg atcaattgtc
    25861 cctgaactgg aggatatata caagagaaag gatctattcg taaaaaacag ccaatggatg
    25921 attggtggag acggatgggc ttacgacatt ggttttggcg ggatcgatca tgtacttgcc
    25981 agcggagcag acgtgaatat tcttgttttg gataacgaag tctattccaa tactggcggt
    26041 caaacctcaa aagcaacacc agcttcggca atcgctaaat tttcggctag tggaaagtat
    26101 gcttcaaaga aagatctagg aatgatggcg atgacatatg gaaatgttta tgtggcgcaa
    26161 attgcttcag gtgccaatca aatgcagacg ataaaggctt ttgaagaagc agaaaagtat
    26221 cctggacctt ctttaatcat tgcctatacg ccttgtatca cacatggttt gcttggtgga
    26281 atgaaagaat cgcttaaaga agcaaaagaa gctgtttctt ctggctattg gtcattatat
    26341 cgatataacc ctgcaatggt cgaaaaaaac aagaacccaa tgactttgga ctacaaaaaa
    26401 cctgatttct ctgctatgcc tgatttcatg aggaaacaag ttcgcttttc ctcgctagag
    26461 aacgcaaatc caaatcttgc aaaagcttta tttgaaaaga ctatccaaga cgctaaaaca
    26521 cgtttttaca actatgctgc actgacggga gaattagaaa aaatcaagaa acgacttgaa
    26581 ccagaggaat ctatcgatgt acccgttaag actagaaaag aaaaaacgac ggaccttgaa
    26641 gcagaagaaa gaagagctgc cagagcagaa cgacgtaaaa aaatggaaca agctacaaaa
    26701 gaaaaaaagg aaaaataaca gaaaaaatat aatgaataat tctctgaagt ttaaaaatat
    26761 ttattgcaga gaaaaataaa acgcatactt gcaattttcc tgaaaattat gcgtttttct
    26821 caagaaaaat acgagcagat cagcctctaa aattaaaaca tatgatatac tagagaggaa
    26881 atctcaaaag agaagagagg ctttttatgt catttcaaat cggggaactt tttaatttgg
    26941 actattggca gcaaatgttt tccggtgact tgttctctct gaattttttg gtgaacatac
    27001 ttgatattct ggttgtctgg tatcttgtat acaaactgat ccaactgctg cgaggcacaa
    27061 aagcgattca gcttctgaaa ggtgtagcta tttttattgt tatccgaatc gtagcggaaa
    27121 tcattggtct gcacaccttg tcttggctga tgaaccaagt cattacgtac ggggtgatag
    27181 ctgctatcgt gatttttcag ccggaaatcc ggagaggact ggaacatctt ggacgcagtt
    27241 cgcttttcaa acaatcaaaa agcgaacagc aggaagatga gaagatgatc ttgtcttttg
    27301 acaaagccat ccaatatatg tccaagcgaa aaatcggtgc gctaattact atcgaacggc
    27361 atactggatt agatgagtat attgagacag gaattgcatt agatgcagat atcactggag
    27421 agctgctgat caatatcttt attcctaata caccgctgca tgacggtgca gttattgtaa
    27481 aagaaggaaa gatcgcagta gcaagcgcct atcttcctct atcagaaagt atgttgattc
    27541 caaaagaatt cgggactcga caccgggcag ctgtcggtat aagcgaagtc agcgacgcga
    27601 ttaccatcgt tgtctctgaa gaaactgggg atgtcagcat tacattagat aatgaactga
    27661 tggctggctt atcccagcaa gaatatttgg caattttgcg tagagaactg attgctgaag
    27721 agaaaacaaa aaacaagaaa aacgtgattc aatctttcct agacggtatg actaagggag
    27781 gggatcgcaa atgatctcaa aagagaaacg gacaagcatt ctttacagtt ttattgcatt
    27841 gctgttcagt attgcgctgt acttcaatgc aaatggccag tccgttccaa atacgctttc
    27901 tggaaatgaa gcgtataatc aaacagttac tggggtgcca atcaagattt cttatgattc
    27961 aaagcgctac tatatccatg gatatgaaaa tgcagtgact gtcaaacttt ccagtgcaaa
    28021 ccgagttcag ttgaatgcag aggcaaacga agatacacgg atgttccgcg tcacagcaga
    28081 tatcactaag ctgggcgaag gaacacatga ggttccgcta aaaattcaaa atttgagcag
    28141 tgctgtcaca gcaaagatcg aaccatcgac tattacagta acggttgaga aaaaggtaat
    28201 aaaagatttc gatgtggaaa cgttgattcc ttcaacgttc acaccatctg gatatgaact
    28261 tgatgatact tccgttagtc ctaaaaaagt ctctatcact acaggtgaca aaacattggc
    28321 agaaatcaaa cgagtagtag ctgatgtgga cgcttcgatg gtcacagatg atgggattaa
    28381 tagtgaggtc ccaatacagg cgctgaatgc agcaggggaa gtattaagta tcgtctctga
    28441 tccagtgcaa gtaacagtga gagctgattc gatcaagcca agcaagtcgg tacgacttta
    28501 tgggatacag caaggaacac cagctgccgg ggtagaaagc tatgatttca gtttttcaaa
    28561 actggaagca gaagtaagcg gctctagcga tttgcttgca tcgatcggtg acagtattgc
    28621 tgtaccgatc aatgtctcag gtatcaatca tcgaacaact cgtaaaattg aaataccagt
    28681 agaagagggg ttaagtatca agcccgaatc agttagtgtg gagattacac ccgtgttgca
    28741 aacgtcaagc agtagctctg cttcgacgag cagtcggaca caagaaaata caagtacgag
    28801 tagaagtatt tcatctgctg attcaggaac ggaaccatcg acaacaacca tttcttccag
    28861 ttcggaatct gggacaagca gctcgaccac aagtacagaa gaaacatcag aaacaagcga
    28921 atcaacacaa atgagcagaa caaattcctc agaaaattaa gttaaaggag taattttaca
    28981 actatgggta aatattttgg aacagacggc gtacgcggtg aagccaacaa ggaacttacg
    29041 ccagaacttg catttaagct gggacggtgc ggaggttatg tcctaagcca gcatgaagaa
    29101 gaaggaaaac gcccacgcgt gttagtaggg cgggatacaa gaatctcagg tcaaatgtta
    29161 gaagaagcgc tgatttcagg acttttatcg gtgggtatcg aagtgttcca gctaggtgtg
    29221 atctctacac caggtgttgc ttatctgaca cgtttgcaaa aagcaagcgc aggggtcatg
    29281 atttctgctt ctcataatcc agcacaagat aatggaatca aatttttcgg tgcagatggt
    29341 tttaaactag tagatgacca agagctagaa attgaagcat tattagatgc tgaagtggat
    29401 gaattgcctc gtccatcagc tgaaggattg ggaactgtag aagaatttcc agaaggctta
    29461 ttgaaatatt ctcaattcct tgtacaaacg atcaacggtg acctttccgg tttgaccatt
    29521 tgtgtcgatg cagcaaacgg agcaacttca acaagtgtta atcgtttgtt tgccgatctt
    29581 gaaacagatt tttataccat gggcacaaat ccagacgggt taaatatcaa tgatggcgta
    29641 ggctctaccc atccagaacg tttagctgag atggtcgttg aaaaaggagc agatgctgga
    29701 ctagctttcg atggcgatgg cgatcgtatt attgcggtgg atgaacttgg aaacattgtc
    29761 gacggtgata aaatcatgtt tatctgtgct aaatacttgg cagaaaaaaa tcgattgaaa
    29821 aaagatacga tcgttacgac tgtcatgagt aacttaggat tccacaaagc ggtggaagga
    29881 atcggtttga aagatgtcgt tacacaagtt ggtgatcgtt acgttgtaga agaaatgcgc
    29941 aaaaacgatt ataactttgg cggcgagcag tctgggcaca tgattttctt agattttaac
    30001 acaactggag atgggatgct ttcaggaatt cagttactga atatcatgaa acagacaggt
    30061 aagaaattat cagagcttgc agcagaggta acaatctatc cgcaaaaatt agtgaatatc
    30121 cgagtaagtg ataaaaacgg tgcgatggat gttccggcaa tcaaaaaagt gattgaagag
    30181 gcagaagctg aaatgggatc agaaggaaga atcctagttc gtccttccgg tacagaacca
    30241 ttacttcgag tgatggcaga agcgcctact gatgagaaag ttaattacta tgtcgataaa
    30301 atcgcggcag tagtcaaaga agaaatcgga ctggattaag agatattttc ataaaaaata
    30361 aaagaacaag gaccagtgtc agatgtctga cactggtcct tgttctttta ttattctact
    30421 gtcactgatt tcgctaaatt acgtggtttg tcaacgtcga atccccgtaa tagcgttgcg
    30481 taatatgcaa gtaattgagt cggtacgact gtggttagag tagttagata agggtggaca
    30541 gttggtagca ccagctggtc actttccttt tctaatccct ctgagacaat aactagtgtt
    30601 ttagcgccac ggctttcgac ttctttcagg tttcctcgag tatgcgcacc agtcacttct
    30661 tcactgatga ttccaatcac tggtgttcct tcttcgatca aagcaatcgt tccatgtttc
    30721 aattcgccag cggcaaaacc ttctgcctgg atataactga tttctttcaa tttcaaagca
    30781 gcttcaagag aaacgttgta gtcagcgcca cgtccaatgt aaaaggcatt tcttgtatcg
    30841 ctgagatata cttctgccaa ctcttctaaa taatcttttt gggcgatgac cgattccatc
    30901 gcattagcaa ctaaactcaa ttcatgagtg atatcgaata caagagaagt ttcaaattct
    30961 tttttatcac cgatagcttt ggctaataaa gttagtacag ctatttgtgc agtataagct
    31021 ttcgttgatg ctactgcgat ttcgggtcca gcatgcaata gcagtgtaaa ggatgcttcc
    31081 cgcgaaagag tagaaccagc aacatttgta atcgtaagtg aagggtagcc gagacggttg
    31141 atttttacca gaacttggcg gctatctgct gtttcgccac tttgactcaa gaaaataaag
    31201 aatggttttt ttgaaagaag aggtgtgtta tagccaaatt cactggctaa atgaacctca
    31261 acagggatct gtgtcaattt ttccagagtc tgtttacctg ctaaccccgc atggtaacta
    31321 gttccgcaag caacgatata gattcgatcg ctatcagaca atgtatcgac cagcttttga
    31381 tcaagagaga cacgattgtc ttctgttacg tatttttgag cgatacgacg catgataata
    31441 ggctgttcgt cgatttcttt cagcatataa aaaggatagg tacctttctc gatatctgaa
    31501 gaatctactt gtgcttcgaa tgaagaacgt tctacgatgt cacctgcaaa attttgaata
    31561 ataacttttt ctgatgtgac ggtaaccatc tcttcatctt caatctcaac gaattccttc
    31621 gtatgagcaa gcatagccat tgcatcgctg gcaatcacat tgaatccatc gccgagtccg
    31681 attaatagtg gacttttatt tttagcgacg taaataacat caggtgctgt ccgatcaatc
    31741 aaagcgaaag cgtaagaacc tttgatgacc cgcaaagctt ttaaaaacgc ctcttgcgtt
    31801 gtagtatcct tcgcgaaagt ttcaattaga tgagcgataa tctcagtatc ggtctcacca
    31861 ataaagtggt catttgctaa ataagcaatt ttaagttctt caaagttttc gattacgcca
    31921 ttgtgaacga gcacgaagcg tccactttgg gaagtatgtg gatgggcgtt ttcttcagaa
    31981 ggttgcccgt gagttgccca gcgtgtatga ccaataccta tatttcctgt agtatcaata
    32041 tttattttgt ttttcaaatt ttgaattctg ccttgtgctt tgaccagata atcagttgtg
    32101 ccatctgaga caaagatacc agctgaatca taaccacggt attctaattt ttctagaccg
    32161 tcaatcaatc ctggtgttac attttttaat ccaatcattc caacaattcc gcacataaaa
    32221 aacatcctac tttctataat gaattcgtat gaatgcttcc agctgcagga aaatcctttt
    32281 gttttatctt tgcagatatt ttttgtggat tttctgtaga gtgcagtccc tagggccatc
    32341 cgccgaatct ttcgataaac cctctcctcg tcatctgttt acacagacct ggcgctatcc
    32401 taaaaggtat aactatcctc ctcttttgtt ttttatctgg aaacatttaa aatcataacc
    32461 ttttgcgaat gttcttgtca acaaaaaaag aataaaaaat aaattggtat atatcaaatg
    32521 ctttgtcaaa tgctcttttt atgtcaaaaa ataatgggtt tttatctatc atctttttca
    32581 ggagttcggt acaatagaaa gtaatcggat agaggaggag taaaaatgga ttcaaaaatt
    32641 gagatcatga cactgggaat gttaaaaaaa caattgtcca aattcgaagc atcagctggt
    32701 gtatcagatg atacgaaaat atttttagat actggctggg atagcatcca agaaatcgca
    32761 cctgatgcct tggaagttgc tgaagctcgt gagtttacgg tagaagatga attgacaaaa
    32821 gagtcgtttt ccgggtatgc gcgggaagaa aaagcagagc ggttcgacac ttctgagcag
    32881 tcagaaactg tgatcgtcat aaaaaacttg tattagaaag gagaaagaat gaataagaga
    32941 cgttgggcag cagttctgat tgctgcagga ttatttgtgg tttcgttagt ttcagctagt
    33001 ttgactaagc ctcaggaaaa cgatcaaatt gagggattga acagttggtt atatggggat
    33061 gaagaactag cttcaaatgt gttggaagag ggagacagta cgcaaaaaat cgtaaaatta
    33121 tcagtagatg gaacgattgc tgcgggaggg tcttcaggtt tatttgcttc tgaaggttac
    33181 aatcataaaa attttatgga acaactgaag gcgattgaag aagatccaac agtcaaaggt
    33241 atctttttag aagtaaattc tcctggtggc ggtgtctacg aaagtgcgga aatcgctaag
    33301 aaattggata cgattcgtaa agaacatgat atcccaatgt atgtcagcat gaaaaacatg
    33361 gcagctagcg gtggctacta tatttctgct caagcagata aaatctttgc aacagaagaa
    33421 acagtgaccg gctcaattgg tgtgatcatg tcaggattga attattcagg acttctagaa
    33481 aaactgggtg tggaagatac aacagttaaa agcggagcgt tgaaagatat gggttcagga
    33541 acgcgaccag agacaaaaga agaagaagcg gtgcttcaag catatatcga cagcgcatat
    33601 agtcgttttg tagcaatcgt cagcgaggga cggaataaat ctgaagaaga agtcaaaaaa
    33661 attgctgatg gaaggattta tgatggtgtc caagcaaaag aagtaggttt agtcgatgaa
    33721 ttgggatttc ctgaagatgc tttaaaagct atgcgaaagg aacaacagct tgaagatgcg
    33781 gaactagtag aatattcttc cacgccatct gggtttgcta gtacatggtt aggaagtaaa
    33841 ctagcagaat tccagggatt gaagccttct gagactagtc agatcttatc cgttcttgag
    33901 aatctaggga cagcggaatc accaaaagca atgtattatt acggaggtga gtaagcatgt
    33961 cagagctttc aaatgaaaat aaggaacaag aaaaagataa gcaacagcag cctgctgtag
    34021 gacctgactc caacttttcc caaaaggtac tgagctcttt tgaaagagag cctttgacgc
    34081 ccgaagaaat caaaaagaaa caacgtaaat ggaaaaagta tacggacaca agaaatgaac
    34141 cgccaaaagc aaatgatttt cctaagtatt tctatgcagg tttttttatc cgaatgtttg
    34201 ctttcttggt cgatcttttg tgtatcggag ctattacgcg tttaacactt ggtattgcca
    34261 aaaatctagg ttggcttaat ttttcagatt cttatctgag tatctacgga ttcacagcct
    34321 tacttattta tctagcgtat tttattttat tgacgaaatt caatcatggt caaacgattg
    34381 ggaaaatgat ctttggtatt cgagtcatca gctttgatga agaagaactt agctggtcaa
    34441 ccgttttagt tagagaaggt gcttgccggt tcattctaaa atttcctttg ctgatgttag
    34501 ggtatcttcc agcaatcttt aatcagaaaa agcaacatct aggcgatttt ttcactaata
    34561 caagtgttgt tacgatcaat ctgatcaaag cattcaacca ggaagtcaac agctgatgag
    34621 caataaaaag cagtaacagt tgaaagcacc ttctaaaagt cagatctaaa atctagcttt
    34681 tagagggtgc ttcagttatt tttaattgtc atgctttttt tgttttgcta ctattgaaca
    34741 tgagctgaac atgagcaaga ggaggacact gcgtgaagcc gattgatttt ccagaaaaat
    34801 atgagaattt aatgcgagta gcacaacaag cactagcaaa ccagcaatat cagcaggcaa
    34861 aagaattatt tcaacgagct tacgagttga aagaaagctt tgaggctaat agcctgcttg
    34921 ttttttgtct ttacgagttg gacgagaaaa aagaagcatt gaagcaagca ctcttgcatg
    34981 aaaaacagta tcttcaaaat gaggagtttg ctgaatttta ttttgacttg ttaatcagta
    35041 cacaagattt tttatatgct agaaaattga tagcaagtac agatttttat gaagctttcg
    35101 agcagcgggt cattgaaaaa atccaatttg cagaagaatt atccggacaa atggagaggc
    35161 agaagatcaa ggcactccac aataaatcgg aggaacttcc ttcgttggaa cctgcacgtc
    35221 agcttgcgtc tattgatgag atcgaacagc ttccttatca tgagtttatc caaacagcga
    35281 gtaaacttat tgtatcgcca gaggtccata ttttagcaag ggcaaaatta ctggagacac
    35341 ttaggcaatt gaacgaatgc catcctgttt tctatttgac gattgaagaa aagttagtca
    35401 aagtcatacc aaaagatctg ccaaagccgc agcagcaggc aagttaccag caattatgtg
    35461 tattttcaga tcgttacggt aatgaagatg cgctgcttag ttccgtgtta aatgaagaat
    35521 tcacgttgca atctgcaatt gtttatcctg tctatgatac atacatcgaa gacccgaagc
    35581 gctggtttca actgactgta gaggcgtgta caggaaaaac gatgtatgaa ggtacagaag
    35641 atgaaaaaca agatttttta aaaaaaagag aaaaaatttt gcagcaaatg atcttcttcc
    35701 attagatttt atcagtgaaa aagataaaaa tgttatgaaa aacaaggatt ctatccattc
    35761 aatatgttta caatttgaaa gggttagtgt actatttaaa ggtatgcatg aaacgttttt
    35821 gatgtataaa atgaatttct aaactcggag ggaattaaat gactgcaaca tttgaaaaaa
    35881 aaggcacgaa tgatggtgtt ttaaaattta cgattgcgca agacgaaatc caaaaaggat
    35941 taacaactgc gtttaataaa gtgaaaaaaa gcttgaacgt acctggattc cgtaaaggga
    36001 aagtttctcg tcaagtattc aaccgtatgt acggtgaaga agctttatat gaagatgcat
    36061 tgaatgctgt attaccagaa gcttatgaag ctgctgtcaa agaagctggc atcgatcctg
    36121 tttctcaacc aaaaatcgat gttgaaagca tggaaaaagg tcaagactgg gtaatcactg
    36181 ctgaagtaac agtgaaacca gaagtaaaat taggacaata taaagatcta gttgttgaaa
    36241 aacaagaccg tgaagtaaca gatgctgacg tggatgcacg tatcaaacgt gaacaagaag
    36301 cacaagctga attagttatc aaagaagatg ctgcagctga aaatggcgat acagtagtaa
    36361 tcgacttcga aggctttgta gatggcgaag cgtttgatgg cggaaaaggc gaaaattact
    36421 cacttgaatt aggttcaggc tcattcattc caggttttga agatcaatta gttggtcata
    36481 aatcaggtga aagtctagat gtaaatgtaa ctttcccaga agattatcaa gcagaagatc
    36541 ttgctggaaa agaagcagtc ttcaaagtga caatccatga agtaaaagca aaagaagtgc
    36601 ctgaattaga tgacgaattc gcaaaagatg ttgatgacga agtatcaaca ttagacgaat
    36661 tgaaagaaaa atatcgcaaa gaattagtag aaacaaaaga aaaagcagcg gacgatgcaa
    36721 aagatgaagc agcgatccgt atggctgttg aaaacgcaga aatcgttgaa ttgccacacg
    36781 tgatggttca tgatgaagta catcgttcaa tggacgaatt cttgaacaac atgcaacgtc
    36841 aaggtatctc tcctgaaatg tactatcaat taactggttc tacagaagaa gacttgcata
    36901 aacaattcga aggtgaagca gaaatgcgca caagaacaaa tcttgtgatc gaagcaattg
    36961 cagctgctga aaatcttcaa gcaacagaag aagaaatcaa tgcagaaatc caagaattat
    37021 cagaaacata caacatgcca atcgaacaaa tcaaacgcgt gttgacagaa gatatgctgg
    37081 aacatgatgt gacaatgaaa aaagcaatcg aattgatcac aggtacagcg acagaagcga
    37141 aagaatagaa atcaagtaat aaatcatttc tgaagaagct acttctagaa aattgtttat
    37201 tcgaaataag ggactgtgac acgattttgt cacagttcct tattttttgt taagagtaaa
    37261 aaatgcaaag gtataggtca gttgatcctt tgttaaacac tttttctttt tactaaacta
    37321 tgttatgata gtaaaagact gtaaagtctt tgatagtttc ttgcatactt tttgcaagtt
    37381 aaggggtgaa gaccatgtat gacaatccaa gtagcaatga gaccgtacgc tgttcttttt
    37441 gcgggaaaac ccaagaagaa gtgaaaaaga tcgtagctgg tccaggggtc tacatctgta
    37501 atgaatgtat cgacctatgt aaagaaatca ttgatgaaga attttatgaa gaagctgtac
    37561 gcgaattcac tgaagtccct aaaccactag aaattcttga agtattgaat aactacgtga
    37621 ttggacaaga tcgcgcaaaa cgctcgttag ctgttgccgt atacaatcac tataaacgtg
    37681 tcaatcagca agcacaagaa aacacagatg atgttgaact gcaaaaaagc aatatctgct
    37741 tgatcggacc tacagggtct ggtaaaacat ttttagcaca aacattagca aaaacattga
    37801 atgtaccttt tgcaatcgca gatgcgacaa gtttgacaga agctggttat gtgggggaag
    37861 atgtagaaaa cattttgctt aaattgcttc aatctgctga ttacaatgtg gaacgtgcag
    37921 aaaaagggat catctatatt gatgagatcg ataaaattgc gcgtaaaagc gaaaatgtat
    37981 ctattactcg tgacgtttcc ggcgaaggtg tccaacaagc cttgctgaaa attctagaag
    38041 gaactgttgc aagcgtgcca cctcagggag gacgtaaaca tccacatcaa gaatttattc
    38101 agattgatac gacgaacata ttgttcatcg taggtggtgc atttgatggc atcgaaacga
    38161 ttgtgaagaa tcgtatgggc gaaaagacga ttggatttgg tacacaaaac caaaaacttt
    38221 ctgaagacga aagtgtcatg cagcatatca ttcctgaaga tttactaaaa ttcggattga
    38281 tccctgagtt tatcggacga ttacctgtta tggcagcttt agagaaattg acaacagatg
    38341 atctggtacg gatcttgact gagccgaaaa atgcgctagt caaacaatat caaaaacttc
    38401 tttctctaga taataccgaa cttgaattcg aacctgaagc gttgagagcc attgcgaaaa
    38461 aagcaatcga acgcaatacc ggtgctcgag gacttcgttc catcattgaa gaaatcatga
    38521 tggatgtgat gtttgatatc ccttcaaatg aaaacatcga aaaagtgatt atcacaaaag
    38581 aagcagctga attgtctggt gagccaacag taatctataa agattcagag aaaaaagcgg
    38641 gttgatgaaa agaggctgcc gtaaaagtca ttttggcttt tgcgacagcc gcttgcttta
    38701 ttcatcacct tgttaaggag agagaccatg aaagtccatc aagctgaaat tatcataagt
    38761 gcagtggcac caaaacaata tccagataca caattgccag aaatagctct ggcaggacga
    38821 tcaaatgtag ggaaatcttc ttttatcaac acattgattg atcgaaagaa tttagcccgt
    38881 acttccggta agccaggaaa gacccagaca ttgaattttt atttgatcga gaacgcatta
    38941 cattttgtcg atgttccggg atatgggtat gcaaaagtat ccaaaactga gcgagcaaaa
    39001 tggggaaaga tgatcgagac atatttaacg actagagacc agctacgtgc ggtcgtttct
    39061 ttagtagatt tgcgtcataa gccttctcaa gaagatatcc agatgtatca atttttgaag
    39121 tattatgata tccctgtaat tgttgttgcc actaaggcag ataagatccc tcgtggtaaa
    39181 tggaacaagc atgagagcga aatcaaaaaa gcgttggatt ttgatcgcaa tgacacgttt
    39241 attctctttt cttccgttac aaaacaggga aaagaagaag cgtgggcagc aatcgaacaa
    39301 gcaattgaat cataaaaaga ataaaaaaga cttaccagtc aaactctttc ataaaagttt
    39361 ggctggtaag tctttttttg agcttaactt acttttgttt cttgtctttt ttcttttcat
    39421 tttgtggctg tggttttaga aatcgatcga tctcttcttt tggtgcaact gttttttccg
    39481 gatcgacagc aaatgattcg aaaagtttat ctaaattatc tgtacgttca aatttcaacc
    39541 ccattgtaaa cacctctttg tttcttttta cttagtctat catgtaatcg tccaaattgg
    39601 tttctaagac ataaagtttt agtttttctt tatcgttggt tataattaaa ttataaaaag
    39661 attttaaaga ggtgagaaaa tgattggcga agataaatta ttgatgacta tccagctcac
    39721 agacttaaat gaagaagaaa aaaaacagga aataagcgat tgggctattt tgacacgtct
    39781 gttgatccag ccaacattta tccgctcttt cacacaacaa gcggatccgt atgatcttcg
    39841 aaagcttcaa gaaaaaataa tgctagcatc ggtaagagac gaaagttggt tagttgtagg
    39901 aaatgacgag aacgaatgta gttttcgctt ggaagataat caattattga taaaaaatgt
    39961 attgtctatc tctgttttca aagaaaagca atttttgatt cgagacttca ttcagaaaaa
    40021 aatgattgct catggggtgt tcgcctatat gcgagcttat agcgagttta tctatcacaa
    40081 tacaaaacag atctctcaac ggctattgtt tgagaaaaag gatgaaatcg aacatttacc
    40141 taaaatgaaa caacaaaacg gggaagtagt agtcgattgc aaccaatttc caggatacga
    40201 tcttttttac caaggtttat gtttcacttc gtgttgggaa atgtattata gtcgctatta
    40261 tcatcaaatc atccctaaac cgatttttct cgacatccaa caagtagaaa aagtgaagga
    40321 attagaaaac gaagtcatat gtatccagct ttatcgagac ccctttaact ggcaaaagcc
    40381 aaacaatcaa atgtttcaat cctattttag ggaccagctt gggtttgatc atcttgcttg
    40441 ggacaatggt gtcggtttat tgaaaccgcc attcgttgaa tacatttaca ctgaccatac
    40501 gatccaatct gtccagtatc agaatgcaca aatgcaacct gttcctaaaa agaatgcttc
    40561 attttttgta acaaaaagtt atgatattca acaaggagat tataaagaaa ggcgtgttcg
    40621 cggaacacta aatgctcagg cgtattttcc ttgggttgac gagaatcgct cgcgcatgat
    40681 gtgctataaa gttatcgatc cgactgttgc tttggataat ggaatcgaag cttattgtta
    40741 ttatattagg gaatatctag aagtagaggt aacagatgaa aaatatcaag agtatctttt
    40801 atccttaaga atctatgttc cttcagaatc cctgtcagaa ttaccgttaa aggaaatcaa
    40861 acatagactc tctgatgtta ccttcaaacg aataaagaaa aagcggcgaa gtatccaatt
    40921 cgatgtcaag aaaggtgagc agcatctgcg tgtgcaattt ttagattacc gagaattaga
    40981 acaattgatt actttacaaa aaatatgagg tgaagaaaat ggataaacaa ctctattatc
    41041 gaataatggt tccggcatca ttattatccg tctgtctctt ttttgttcct aattttccta
    41101 ttcgatcagt catcggtttt ttggtcatgt ttggcgggat ggga
//
LOCUS       DAGCWU010000007        40781 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.007, whole genome shotgun sequence.
ACCESSION   DAGCWU010000007 DAGCWU010000000
VERSION     DAGCWU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.007"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(105..629)
                     /locus_tag="MBZ71_000292"
     CDS             complement(105..629)
                     /locus_tag="MBZ71_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4428274.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="MBZ71_000293"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="MBZ71_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBT4428275.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="MBZ71_000294"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="MBZ71_000294"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBT4428276.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="MBZ71_000295"
     CDS             complement(2351..3031)
                     /locus_tag="MBZ71_000295"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBT4428277.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="MBZ71_000296"
     CDS             3157..4026
                     /locus_tag="MBZ71_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4428278.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="MBZ71_000297"
     CDS             4052..4567
                     /locus_tag="MBZ71_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428279.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="MBZ71_000298"
     CDS             complement(4614..5207)
                     /locus_tag="MBZ71_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4428280.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="MBZ71_000299"
     CDS             complement(5232..5411)
                     /locus_tag="MBZ71_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428281.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="MBZ71_000300"
     CDS             complement(5488..5997)
                     /locus_tag="MBZ71_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428282.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="MBZ71_000301"
     CDS             complement(6093..6473)
                     /locus_tag="MBZ71_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4428283.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="MBZ71_000302"
     CDS             6803..7552
                     /locus_tag="MBZ71_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBT4428284.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="MBZ71_000303"
     CDS             complement(7736..9013)
                     /locus_tag="MBZ71_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428285.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="MBZ71_000304"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="MBZ71_000304"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBT4428286.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="MBZ71_000305"
     CDS             complement(9935..10879)
                     /locus_tag="MBZ71_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBT4428287.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="MBZ71_000306"
     CDS             complement(10881..11567)
                     /locus_tag="MBZ71_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBT4428288.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="MBZ71_000307"
     CDS             12041..12361
                     /locus_tag="MBZ71_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBT4428289.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="MBZ71_000308"
     CDS             12515..13420
                     /locus_tag="MBZ71_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4428290.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="MBZ71_000309"
     CDS             13417..14190
                     /locus_tag="MBZ71_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4428291.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="MBZ71_000310"
     CDS             complement(14371..15795)
                     /locus_tag="MBZ71_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4428292.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="MBZ71_000311"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="MBZ71_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBT4428293.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="MBZ71_000312"
     CDS             complement(16517..18499)
                     /locus_tag="MBZ71_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4428294.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="MBZ71_000313"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="MBZ71_000313"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBT4428295.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="MBZ71_000314"
     CDS             complement(19412..20473)
                     /locus_tag="MBZ71_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBT4428296.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21250)
                     /locus_tag="MBZ71_000315"
     CDS             complement(20684..21250)
                     /locus_tag="MBZ71_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4428297.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="MBZ71_000316"
     CDS             complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="MBZ71_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBT4428298.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="MBZ71_000317"
     CDS             complement(23038..24159)
                     /locus_tag="MBZ71_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBT4428299.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="MBZ71_000318"
     CDS             24285..25226
                     /locus_tag="MBZ71_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBT4428300.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="MBZ71_000319"
     CDS             complement(25376..26272)
                     /locus_tag="MBZ71_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBT4428301.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="MBZ71_000320"
     CDS             26541..27881
                     /locus_tag="MBZ71_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4428302.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="MBZ71_000321"
     CDS             28099..28542
                     /locus_tag="MBZ71_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBT4428303.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="MBZ71_000322"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="MBZ71_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBT4428304.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="MBZ71_000323"
     CDS             complement(30867..32090)
                     /locus_tag="MBZ71_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBT4428305.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="MBZ71_000324"
     CDS             complement(32121..32960)
                     /locus_tag="MBZ71_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBT4428306.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="MBZ71_000325"
     CDS             complement(32977..33657)
                     /locus_tag="MBZ71_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428307.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="MBZ71_000326"
     CDS             complement(33659..34732)
                     /locus_tag="MBZ71_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4428308.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34788..35026)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35069..35302)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35379..36050)
                     /locus_tag="MBZ71_000327"
     CDS             complement(35379..36050)
                     /locus_tag="MBZ71_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4428309.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="MBZ71_000328"
     CDS             complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="MBZ71_000328"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBT4428310.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="MBZ71_000329"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="MBZ71_000329"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBT4428311.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40562)
                     /locus_tag="MBZ71_000330"
     CDS             complement(39933..40562)
                     /locus_tag="MBZ71_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator, Rgg/GadR/MutR family
                     domain-containing protein"
                     /protein_id="HBT4428312.1"
                     /translation="MLDRVQEDSFDPYKEISNNRRHFKKTEDLYYLVLNLHMVEKTEV
                     SETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFPWIFSDRFIENNYLKIKKRMRR
                     LAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNEDMKRSLAHKERSTVIYETLMAD
                     YYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSLEKKLLAIAEPSN"
BASE COUNT        11735 a         8573 c         7217 g        13256 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gtgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaagga
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga t
//
LOCUS       DAGCWU010000008        37481 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.008, whole genome shotgun sequence.
ACCESSION   DAGCWU010000008 DAGCWU010000000
VERSION     DAGCWU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.008"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..556)
                     /locus_tag="MBZ71_000331"
     CDS             complement(<1..556)
                     /locus_tag="MBZ71_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBT4428313.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="MBZ71_000332"
     CDS             complement(572..1051)
                     /locus_tag="MBZ71_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBT4428314.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="MBZ71_000333"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="MBZ71_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="MBZ71_000334"
     CDS             complement(2098..3204)
                     /locus_tag="MBZ71_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBT4428315.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="MBZ71_000335"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="MBZ71_000335"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBT4428316.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="MBZ71_000336"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="MBZ71_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBT4428317.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="MBZ71_000337"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="MBZ71_000337"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBT4428318.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="MBZ71_000338"
     CDS             8175..8873
                     /locus_tag="MBZ71_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBT4428319.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="MBZ71_000339"
     CDS             complement(9041..11680)
                     /locus_tag="MBZ71_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4428320.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="MBZ71_000340"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="MBZ71_000340"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBT4428321.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..14698
                     /locus_tag="MBZ71_000341"
     CDS             13523..14698
                     /locus_tag="MBZ71_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4428322.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            14702..15343
                     /locus_tag="MBZ71_000342"
     CDS             14702..15343
                     /locus_tag="MBZ71_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428323.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            15340..16257
                     /locus_tag="MBZ71_000343"
     CDS             15340..16257
                     /locus_tag="MBZ71_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4428324.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            16254..16925
                     /locus_tag="MBZ71_000344"
     CDS             16254..16925
                     /locus_tag="MBZ71_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428325.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(17466..17954)
                     /locus_tag="MBZ71_000345"
     CDS             complement(17466..17954)
                     /locus_tag="MBZ71_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBT4428326.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(18155..18421)
                     /locus_tag="MBZ71_000346"
     CDS             complement(18155..18421)
                     /locus_tag="MBZ71_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBT4428327.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(18421..18684)
                     /locus_tag="MBZ71_000347"
     CDS             complement(18421..18684)
                     /locus_tag="MBZ71_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4428328.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            19160..19345
                     /locus_tag="MBZ71_000348"
     CDS             19160..19345
                     /locus_tag="MBZ71_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4428329.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(19487..21310)
                     /locus_tag="MBZ71_000349"
     CDS             complement(19487..21310)
                     /locus_tag="MBZ71_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBT4428330.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(21908..24613)
                     /locus_tag="MBZ71_000350"
     CDS             complement(21908..24613)
                     /locus_tag="MBZ71_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBT4428331.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(24919..25866)
                     /locus_tag="MBZ71_000351"
     CDS             complement(24919..25866)
                     /locus_tag="MBZ71_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBT4428332.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            26056..26325
                     /gene="rpsN"
                     /locus_tag="MBZ71_000352"
     CDS             26056..26325
                     /gene="rpsN"
                     /locus_tag="MBZ71_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBT4428333.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(26501..26863)
                     /locus_tag="MBZ71_000353"
     CDS             complement(26501..26863)
                     /locus_tag="MBZ71_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4428334.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(26874..27995)
                     /locus_tag="MBZ71_000354"
     CDS             complement(26874..27995)
                     /locus_tag="MBZ71_000354"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBT4428335.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(28011..28361)
                     /locus_tag="MBZ71_000355"
     CDS             complement(28011..28361)
                     /locus_tag="MBZ71_000355"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBT4428336.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(28491..30002)
                     /locus_tag="MBZ71_000356"
     CDS             complement(28491..30002)
                     /locus_tag="MBZ71_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBT4428337.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(30324..31676)
                     /locus_tag="MBZ71_000357"
     CDS             complement(30324..31676)
                     /locus_tag="MBZ71_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBT4428338.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(31829..32905)
                     /locus_tag="MBZ71_000358"
     CDS             complement(31829..32905)
                     /locus_tag="MBZ71_000358"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBT4428339.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            33101..34846
                     /locus_tag="MBZ71_000359"
     CDS             33101..34846
                     /locus_tag="MBZ71_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBT4428340.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(35126..35563)
                     /locus_tag="MBZ71_000360"
     CDS             complement(35126..35563)
                     /locus_tag="MBZ71_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBT4428341.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(35547..36920)
                     /locus_tag="MBZ71_000361"
     CDS             complement(35547..36920)
                     /locus_tag="MBZ71_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428342.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(37242..>37481)
                     /locus_tag="MBZ71_000362"
     CDS             complement(37242..>37481)
                     /locus_tag="MBZ71_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4428343.1"
                     /translation="KLYLLNKVRGQGLTSSVFRWYEELAAQTKEREIFLRVNQGNHQA
                     IEVYKHEGFEITEELISDIGQGFQMVDYKMKKALA"
BASE COUNT        10625 a         8052 c         6899 g        11905 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg
    14161 tgtgatccaa tttgatacac cagaaaatat tttacgtcat cctgcaaacg atttcgtaga
    14221 agagttgatt ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga
    14281 agttatgcta aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa
    14341 actgatgagg gaaaaacgtg tagatacgtt gttagttacc gataattcga atgtattaaa
    14401 aggatttatc gatgtagaaa cgttgaataa aaaacgagga aaagtatcca gtgtcggcga
    14461 cattttgaat aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg ataccttgca
    14521 acgcatttta aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt
    14581 aggtatcctg actcgtgcat cattagtgga tatcgtctac gatgtgattt ggggagaaga
    14641 tcccgcaact actgatgttg cagagtcagc tactcctgaa gaatctaagg aggtctgacc
    14701 tatgcagcag ttttttcatc agcacggttc agaaattctc tcaaaaggtt ttgaacatct
    14761 ttatatttca ggacttgctt tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt
    14821 actgacacgt tttccaaaag ctgccacgat tgtgattggc ttaaccagtg ccttgcagac
    14881 aatcccttct cttgctttat tggcattgat gatcccgcta tttggcgtag gtaaattacc
    14941 tgcgattatc gcactcttta tttattcatt attgccgatt ttacgtaata cgtacattgg
    15001 gatgaaaaat gtcgattgga attatcggga tgtggcaaaa ggcatgggga tgactgagtt
    15061 ccaatccatt ttttccgttg aattgcccat tgctatgcca acgatcatgg ctggtattcg
    15121 tttagctgcc gtttacgtga ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg
    15181 tttaggcgat ttgattttta gtggactgaa taattaccaa ccagatctca ttttggcagg
    15241 tacgatccct gtaacaattt tagcactatt ggctgattgg cttctaggtc tcttggaaca
    15301 ccggctgaca ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat actaaaaatg
    15361 accatggttc ttttctctct tcttatactt gcgagttgtt ccttccctgg tcttgccagc
    15421 aatacagatg aagacaccat ttcgatcaca ggtggaatca cttcagaagc acagatctta
    15481 gcaagtctag ttgccggaat ggttgaacat tatacagata aaaataccgc aatcatcaac
    15541 aatctggcta ctacaactat caaccatcaa gcgatgatga acggagatgc ttcaatttca
    15601 gcggctcgct acacaggaac agatttaacg actacattga accttcctcc tgaaaaagat
    15661 ccgaaaaaag cgtttgctac cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg
    15721 ttcccgtcct atggttttga aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa
    15781 aaataccatc tatctaaagt cagcgatctg aaaaatgtag cggatgaatt agtagctggt
    15841 gtagatactt cttggatcaa tcgtaaagga gacggttatg acggcttcca agaaacttat
    15901 ggcttctcct tcaattcgat attacctatg cagattggac ttgtttatga cgcagtcgaa
    15961 gcaggaaaaa tggatattgt tcttggctac tctacggatg gacggatcgc cagttatgat
    16021 ctagtcatgt tggaagatga tcgtcaattc ttcccaccat atgatgctgc tccagttgta
    16081 gacaacaaga tattagctga tacgcctcat ttaaaagaag ccttaaataa gttaggtcat
    16141 accatcagta cggaaaaaat gcaggaattg aactacgaag cagataataa tctagtggaa
    16201 ccatcagtag tagcacaacg tttcttacaa gaaaaccact attttgaagg gaagtgaagc
    16261 aattggataa aatgaatgtc tttcaacaat ttttctatta tttccaggaa aatggctcct
    16321 atatctttgc acaatttatc cgtcacttcc tgatttctat ctatggtgtc ctctttgcag
    16381 cagttgtcgg gattccggtt gggatcatga tttctaggag acgtaaatta gcaaactggg
    16441 tgattcgttt ggcgaatatc atccagacga tcccttctct agccatgatc tccattttaa
    16501 tcattggatt aggcctggga gtcaatgtcg tgattgttac tgtatttttg tattccctct
    16561 taccgatcat caagaatacg tataccggga tgatccaagt agacaaaaac atccttgatg
    16621 tcggcaaagg catggggatg actgctagac aacgactatt catggtagag ttaccactct
    16681 ctgtttctgt tatcatggca ggcataagaa acgcgttagt tgttgccatc gggataaccg
    16741 cgatcggtgc attcgtcggt gctggtggtc ttggagatat cattatccga ggaaccaatg
    16801 caacagaggg cacatcgatt attttagcag gtgcattgcc aactgcatta atggcgatca
    16861 tcacggactg gcttttaggt attttggaaa gacggctaga tccagccagc cgtacatcac
    16921 gctgatgtta ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt
    16981 ttagttctgt catttttaag gcaataatcc actctgattc ttccctcctt atgggcaaag
    17041 agttgctttt cttagctatt tatccattcc gtatactcca acttcatact gccaacgggt
    17101 tcatatctgc atccaccgac taagtttttt tcttatccgc taaggcattt tgccaatcca
    17161 ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc gtctgcttgt
    17221 tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta agccttttcc
    17281 atcagtcctc gattgttcga tgccttcagc tgcatcatca aaactggtga ctgccgtatc
    17341 ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct tggctaaaaa
    17401 catatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa accggtacac
    17461 gattcttatg gactgcatcc gtcgcaacta gacttggcga tacgatgatc cttcaccaga
    17521 agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac attggcgtaa
    17581 aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc cgaaagcgcc
    17641 acgactttaa cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc
    17701 cgctctgcta aaggaacgac actggtattg tatttcgtat ctgtatcttt tccaaaagac
    17761 catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc cattgctttt
    17821 ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt cgcctcactt
    17881 tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc aattttactg
    17941 attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca ttccattcct
    18001 aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag aaatcatgca
    18061 ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg ggagactctt
    18121 tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc caggcagaat
    18181 aaatttctac aactttattt tcctcatcaa ctttgtaaac cactcgatgc tgtctattca
    18241 gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca
    18301 tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt
    18361 gcttgatttt tcttaaatcc actttcgcag agtttttgat tgcgactgta tagttactca
    18421 tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg
    18481 gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt gaatcgctct
    18541 ccagtcttcc agcccaatca atacagcatt gtttccacta cgatcactaa taatctcgat
    18601 ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac gagcagtgct
    18661 aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa ttatagcgta
    18721 cttgattaag tacgtcaaaa aaatacaata aagcacttaa attcagcaaa cttttcctct
    18781 tttttacata gagaaaaaga ccccttttta gggaggatct ttgtccacgc tcacttctta
    18841 ctcctttacg actagcggta gccaaacgta aagtaattgg taacaacgta tcatctttag
    18901 caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc
    18961 gttgcagtgt gacttgattg atatcttgaa taacctgagg atacgcacga aagatttgta
    19021 acggttattc aaaactgatt ttggccgtca agtcttctac tgcctcaaaa atgattgcct
    19081 ctttcgtctc ctgtcattca tgttcactat cgatttgata cttgcttaga ccgttgggtt
    19141 tgtgaatcac caattaaaaa tggcactgag ttatggtaac aaaaaatggt cacttgacga
    19201 ggctgagatt cctacttatg aagacacatg gcacttaaga gtcgaggtaa aggaagaagc
    19261 tgaaaatata ggaggctgta cattatttgg caaaggattc ggaaattatg atcaagacat
    19321 caaaatcaaa atgttttaca gctaaatagg aagaataata acatttcata cgctgttccc
    19381 atctttaatg gcttatagag ggcaccttta gctgagaata agacaaaaca gaccctctag
    19441 cttcaattaa gagctagagg gtctgttttt gattttaaaa cttggtttat ttcttcaaat
    19501 ggtagctata ggtgctgaca acgatatttt cacgccaaga caatcgcaat cttaaccgca
    19561 aacttgtttg gttatggaga atattctgcc agcgtctgtt tggtacaaat tgaggaatca
    19621 acacagtagt tgtatagttt tgtttcgctg catttttact tacgatatct acataacgga
    19681 taattggatt ttcaatcgag cgataagaag agtggacgat tgaaaaacgg acatctggga
    19741 aatgcttttt aaattctgct tcgatttctt tttctttttc gacatcttcg tctagcgaaa
    19801 catgcatcgc aacgacataa tcaccgatcg aacgtgcgta attcaacgca ccgatattta
    19861 cccgtgtcac attcccgact aatacaagaa cggtattccc gtcataatca tgtagttttg
    19921 catcattttc taaacgaagc tgttctgcta cttttttata atgatcatgg atcttataaa
    19981 aaatgaacat aacgattggc ataatgatga agaaaggcca aatgtcccct aaccgataga
    20041 caaacaaaat cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt
    20101 tgcttaacca tctttttcct tcttttttcc atttgacgac catccctgtt tgagataaag
    20161 caaatggaat gaacacgccg atagagtata acggaatcaa gcgttctgtt gagccatgga
    20221 aaataaacag caatacgatc gaacctagcg ctagtgtgat aatcccattg gaatagccta
    20281 gtcgatcccc acgatcttga tacatgtgag gcatgaattt atcttttgcc agattgtatg
    20341 caagaactgg aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag
    20401 caaattgaag gacgtaatac aaaataccat gtccaaagac agctttacca atttgagaca
    20461 aaacagtgac ttctttttca ggaacgatcc cataccaata gttgataaat gtgatcccca
    20521 cgaagaagaa acctaagatc aatgccatca tcgtcaatgt agcagcggca ttttttgctc
    20581 ttggcttctt aaagaatggt acagcattac taatcgcctc caccccagtt aaagaggaag
    20641 aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac acccggcaca gccgctcctg
    20701 gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat attatataaa ccaacaacga
    20761 tcagtaacga gatcacaagg ataaatgtgt acaccggaaa taataggaac gatgcagatt
    20821 ccctcagccc acgaagattc agcatcatca gtagcaaaac gattgtcaca gaaatagcga
    20881 cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct gctgagacag
    20941 atactgctac tgtcaacatg tagtcgatca ataaagagcc accggaaatc aagccagcat
    21001 tctttccaag attctcactg ctgacgacat atgctccccc gccatgagga taagcatgga
    21061 tgatttgacg atatgatagc gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg
    21121 gtagcgaata ccagatggct gcagcagaca aagcgactaa aacgacaacg atttgttccg
    21181 taccatatgc aatagatgat aacgcatcag acgacaataa tgccaaagca gcaaatcggg
    21241 ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt acctactaac aaccttttta
    21301 agtaatccaa tttctaaact cctttacaca caattttact tcaagtttta tagtcgaaaa
    21361 tttatagtaa acaagatgtt atagtacgct cactgacctt aaaagtcaat aaactttaag
    21421 aaaataattg gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt taatacagca
    21481 aaaatctaac atttttttat gggctttttc tttgaattct ataataaaag gatagaactt
    21541 gacttagaaa atcaagttct atcctttttg atatcttaac ttttggaaga gagatgtaag
    21601 gctgctttct acccatcatt cttactactc gaagcatagc gcagttgtca caacctcttg
    21661 ataaacggtg tttaaaagct tgcatctgcg gtaataagct caaccaaatt aaaaacataa
    21721 gaagctgttt ttatttggtc gttcttatta cttgatgcag agcagctttt ttacaacctc
    21781 ttggtacacg gtgttcaatc agctgaccct cggtattagc tcaaaaaaac ggcaaaatat
    21841 gaggaactat ttttccattt ttttctctaa tactcaggtc aaaacactga tttcacaacc
    21901 tcttttttta atgaaaaata tggctctcat gcgccgcttc tttgaatttc gtcatttttt
    21961 cttcgatgaa cggatagatt gccgttccta cgattccaaa gacaacaaac aaaccgatca
    22021 tgatccagat tgctttcgta gcatttggcc aataaattcc tcctgcagat tcccgtaaca
    22081 agtttaccgc atgagtaaat ggcaagaacg gattgatcat ttggaagaat ttccctgaga
    22141 cttggatcgg atagttcccg cctccgccag agatcgacag tactaagatg ataatagcga
    22201 tccctttacc gacattatcg aataatgcag ccaagacata gacgatcatc atgaacgcta
    22261 aggcgatcaa gacagcaaat gacacgctgt agaccggatt ttggacatct actcctagta
    22321 agaaaatatt ccctaatgta acgatcaagc tttgcgcaat cgccattgtc aaaaatgtca
    22381 gcatacgagc aacgaattgt tctcgtttcg taaaattgtt tcggtctttc tttcctaaat
    22441 agtattctgt cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga catagtgcag
    22501 tataaaacgg ggtgctagct gaaccgttat ttgcgattgg ataaagcgca ttggtttgta
    22561 gctcaacagg ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc aataatttga
    22621 tgacttcgcc tagatcgact tcttgttcgc cttttcggat cgcgtctgct gctttttgga
    22681 taccagcttt gatatttggc cagtcattgt tgatcaagtc cgcagcagcg ttcacagctg
    22741 tttcgacttc tggcattttt tcatttactg ttgtcaatgt accggtgatt tcatttttta
    22801 gtgctggcca atcattttgg ataaaatcag cggccaatcc taatttttgc tgaatgatcg
    22861 gtaattcatt gttgtatagg tctgcccctt cattgatgcc attgacgatg gtatccatat
    22921 ttccattcag cattgtattg gcatcatgga tttcttgtcc aattgcaggc atttcttttt
    22981 gatatttttc taaaattgcc actgcattcg ttacagttgc tttcgttgag ctgagaagcg
    23041 aatcaaagtc gatctggtcc gctttgctca gtgcgccttg tgcagtccca atcgtatcga
    23101 tgacttggtc tagtagagaa gtgacagtgg aactgatttg acctgtattg ataccagcaa
    23161 tgatgccatt gatattatta acaacggcat caatttgatc tagcaattca tcaatagcct
    23221 tcgtgtcttc attttgaaca gctgtgttca aagcagatag gcggttttgc agattgctta
    23281 gcaagggttt taattgattt agctgatcaa taattggctg taatttcgta tttccagcac
    23341 ttgtttgcaa tttagtcagg aactcgacca gtttatcgat tgcattttgt tgttgagata
    23401 ttccgtcaat aaagccgttc aatacattgc tgatgttttt cttttcatca tcagtcagct
    23461 taccatcttc aatagctgtt cttacagtat ctattaacga acctgtcgtt gttgccactt
    23521 gtccaattga cttcaatgta acatctacac tgtttgtgat gcttggtatc gcatctttca
    23581 gctgagaggc acctgatttt gtttggtttg ctagatcact tgcctgattt tctaaatttt
    23641 tcacatctgg caagatatct tgtacttgtt ggatgatcgt caagccttgt tttgcttcat
    23701 cgattccttg gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc tctgctagtt
    23761 gcttaccggc attttggatc tctgggattt tttcttgcaa atcaaggatg atttttgctt
    23821 gatctttcaa ctcaggcatt ttgtcattca atgcaacgac tttttcaccc atctcatcta
    23881 ctttagggat gtaatccacg aattcatttg ctttattcag tttttccttg atttcaggta
    23941 gttgactttg taattcgagg acttgttttg tgtagccgtc gatcgtgtcc aaattttcat
    24001 ctgttgataa aatcatatct ttaactttat tgatacttac taggttcgta tcgatgtcgt
    24061 aacctatctc attgaatact ttcaataaag tcgaactggc tgttttgata aattcatttg
    24121 tgatttgcga ttggatcgaa cttgctcctt tatcggtgat tttcggtgca atcgcattga
    24181 ttttttcatt taccgtatat tcgatttttg gtttcttgat atcaccacta gtaaaactca
    24241 gcagatcttc tgagaaatcc tttggcaagt aaatacctgc gtaatacttt ccggaacgga
    24301 ctccgtcttc taattgttct tttgaatcaa cgaattgcca tcctaattgt ttatttttat
    24361 gaagcgattc gatgacttgc tccccaatgg ccacttcttt accttgaaac tctgctggct
    24421 tgtctgcact gtaaacagca attggcagct ctccagtatt tccatatgga tcccataatg
    24481 ctttgatgtt aaaccacgca tatagcgaag ggatgatcat gatcgcgatg atcagaagtg
    24541 ttgcaatcgg gtttttgaaa atccgtttcc agtcaagcaa atataatcga agcgtatttt
    24601 tgattgactg catagttctc ccccttcttc ttttttcagc tgaattactt taccatgtat
    24661 ttcatacttt tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca tatcacggca
    24721 attgtctcat tgtaatctct tacatattga gaaataatta aggattacag cctttacttt
    24781 atcatgaaat aaaagggaaa gataatttta tgccaaagtt tttatctatt atacgtatac
    24841 gcaaaaaagc taaaacattt tttgtctttg cccctacaaa aatgtttcag cttttttcgt
    24901 tttttattcc tgtatttctt atttttcgaa tgacaggatg attttcccag caggaggatg
    24961 tccttcaact agctgatgtg cttggatgac atgcttaagt gaagcagaca gattcattga
    25021 aataaaaaca tgaaaggctc ctttttggta ggctttgatt atgccagcaa aggcttctgc
    25081 atctagatat tcctttcttg gaacaaacga ttcataaaag ccttcctttt tttgccgcaa
    25141 atctagatct gggaggtcat ttaatgcaac atacctgccg cctggtttca tgatctggat
    25201 gccggtttct cctttgatac ttcctttcgt cgcatcgatt acaaggtctg cctgatcagc
    25261 aaattgcaga cctggatttg ttttatcata agcagcaaat gctgaagctc ctaatttttt
    25321 taccttttcc tcatttttac tggatgcact ggttaagatt cggattccct tttcatggag
    25381 aagctggatc agacttgacc cgaccgcacc tgatgctcct agtatcatta cagtatctgt
    25441 cggctggatt tccgtcaaat ggttgattag attataagcc gtaattcctg gcgtgaccat
    25501 tccggctgct tcttcccaac tcattttatc tggaattttt gctacttttg ctgatggcaa
    25561 cacgacttct tctccgtaag tcccgccgac tgcatggaca gctactcgat cacctacatg
    25621 aacagttgtg acatctgaac caatctctgt cacaatacca gcgccatcat tacctggtac
    25681 ataagggaat ttcaatgttc gtatctcttt catttctcct aaacgcaagg caacatcata
    25741 aggattcaca ctgaatgctt ttatttctac acgaacatgt ccagaaccga cttctctggg
    25801 atgagcatcg atttcttcaa aaacatcttg tgcttctccg taaccgttaa ttgcaaatct
    25861 tcgcatggtc accatctccc tttttcttta agtttacgtt tatgtcttta ttttgacaaa
    25921 aaaaatgtct tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta
    25981 acgtgttaaa ttatttatct aaaagcgtaa agattacgat ttacgctaat aaaaaataat
    26041 tggaggaatt tgttgatggc taaaaaatca aagattgcca aagcaaaaaa acagcaagaa
    26101 acgattgaac gctatgctgc tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc
    26161 ttgcgtaaac ttcctttaga tgctcgtcca gaacgattga aaaatcgtga tttaatcgat
    26221 ggacgccctc gcggatacat gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta
    26281 gcacataaag gtttgattcc aggagtaaaa aaagccagct ggtaatccaa gtctggctgt
    26341 aatgtaaagg aggagggcgc atggaccact ccttggatca tcaaaaaaga tacgttcgct
    26401 tcttctagca gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc
    26461 cttttttata tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca
    26521 gtgcttcgat acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat
    26581 ccaaatggca gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca
    26641 ggataacgga gtctctttct attcctgttc cagtagcttt gatcccaatg tgagtcggaa
    26701 gcttaggttt agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt
    26761 tacctagatt attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga
    26821 ctggagaaag atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct
    26881 tgatattctc ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct
    26941 aattgatctg ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc
    27001 acttttgttc cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca
    27061 cgcccaatcg tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt
    27121 ggccagccat ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata
    27181 tatgtttctc catagccgat tccttcacct gctgggagtt ttttgacttg aatcaattca
    27241 gaaaccagct caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg cccagaggga
    27301 ttaagtccgt acattgctac tccataacga atcatattgc cgattgtttc atcatgccat
    27361 aaagccgttg cactattgct gacatgtaca tatcgaggaa gttcgggaag ttttttcagc
    27421 acttctataa agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta
    27481 gaaaaatggg taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct
    27541 gcctctttta cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt
    27601 agatgaatcg aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcttgc
    27661 aaccattccc gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg
    27721 tcgtaaggaa ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg
    27781 actccttcat ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca
    27841 gctgtttcaa tagctccatg accgtatccg ttcgctttta ctactgcaaa caattctttt
    27901 ccttgcggaa gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc
    27961 actgctttcg ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc
    28021 aaaataattt gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tacattgcct
    28081 tcatgaggtg aacgcgtcac aacaggctga ccattttcat tttttaaaat ctcgacatct
    28141 tgaaaggtaa cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg
    28201 taacgcccgc ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt
    28261 tcatcagagg tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc
    28321 ctaggaagtt cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat
    28381 aaatttagaa cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa
    28441 ataacaaaaa tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta
    28501 tttgaacgga ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc atctttttgg
    28561 ctgttattat ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca
    28621 cgacgattac ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc
    28681 attggcaatg ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct
    28741 gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct
    28801 gctgtttgca ggtatttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact
    28861 gcagcaccta attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc
    28921 cgttttttcg tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa
    28981 gtcacagaca taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt
    29041 tctggatctt gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca
    29101 cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat
    29161 acgctcattc gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt
    29221 gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt
    29281 aattcaggtg tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt
    29341 gcgcggacat agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca
    29401 tgatgtggat ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct
    29461 gagaaaagta atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc
    29521 aagaagccca tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacggttcca
    29581 agtttcaatg tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc cacaacgata
    29641 tgtggacgat ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct
    29701 tgtacacgta cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct
    29761 aattcacgtg ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt
    29821 tctaacattg gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata
    29881 acgtcttttc cagctaatgc tagtggaatc gtttctgctt ggataggcgt tgcttcttca
    29941 aaaccagcgc gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc
    30001 aaatgaatcc tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat
    30061 ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc
    30121 ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa
    30181 acgggtcgcg ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg
    30241 gcaaaaccag tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc
    30301 gttttctgca agtttacaga tttttatttc atttgctgaa gttgttctac cacttcaaat
    30361 aagcccatgc tgttgctgcc ttttagcata atactatctg ttgctgcaag attttgtttg
    30421 atagcatcaa tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat
    30481 ttttggttca attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg
    30541 aatttctcag gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct
    30601 aattccagca tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa
    30661 gtatctaata ccagtcccat ggctgttgga tttgcattgt agacatcgct caaaatagct
    30721 gcaccatttc ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc
    30781 ccttggcgta tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa
    30841 gcattcgtta cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca
    30901 aaatcggttt gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata
    30961 ccgaatgttg tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct
    31021 gcgggcacaa cgagtaatcc gtctgataca agtccgtcag tgatttccat tttggcttta
    31081 gcgattcctt ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct
    31141 gcagcttttg gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc
    31201 atttccaaaa cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca
    31261 atattattgt tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac
    31321 gcggttagat ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgcg
    31381 cctacttttt ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca
    31441 ggaataactg gaaaattttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat
    31501 gctccgtttg tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga
    31561 acgaataaac ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc
    31621 agtgcagagc attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag
    31681 atctctccta aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact
    31741 ctcacttcca agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca
    31801 atcactccag cctcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct
    31861 gatggtatcg attcattcct aactggatca gttcttcaat caaatcaccg tatttcaagc
    31921 ccatattttc ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta
    31981 attcattcag gaataattca tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc
    32041 ttccacctaa catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt
    32101 ctggcacttc ggctggaatc tgcatttcga ttttattatt gatatatttt gcttcataat
    32161 catagaatgc tacgtctttt acgacttcac caggcaaagt cgtccgaacg tcttcatttc
    32221 ctaatacagc aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat
    32281 catactgata ggctgttgct aaagcatttt gcagctcttc tcggttttct gcttttgtaa
    32341 tgccgacact agaacccata ttcgccggtt tgacaaacat cggataaagc aaagaacctt
    32401 cacattgatc aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat
    32461 aaggaacttg cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg
    32521 cacatgcact ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc
    32581 cttggatcgt tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg atggctcctt
    32641 cttctttgat ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac
    32701 ttgggtccca tgaaagatgc aagacatctt tgctggcagg tttttctgtt agtaatggac
    32761 ctttgaccca ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat
    32821 aaatggcatt taaaactgaa aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc
    32881 cgccatatag taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga
    32941 tttactctca tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg
    33001 agccagcttc taaaagaacg agcgtcattt attttttgtc agctagatta tttgtcccgt
    33061 tttttataaa aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg
    33121 cagccgcttg gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt
    33181 acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat
    33241 caagatagga cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac
    33301 attcaaaaca ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat
    33361 cgaaacagaa ctttctcttt cattatcaga tatattcgat gatttttcaa aatcgccttt
    33421 tgcctctgct tctatggggc aggctcatcg tgccaagctg aagaacggtg acgcggtagt
    33481 cgttaagatc caacacccta acatcgccga agagatccga cttgatcttc aactatttga
    33541 acgagcgatc cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt
    33601 actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa
    33661 tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta
    33721 tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa
    33781 tcatttaatg aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca
    33841 gctgaaacaa gaagttgcaa agcttttagt tgagaacttt atgaaacagg tgtttgatga
    33901 tggttttttc catgctgatc ctcatccagg gaatctgcta ttccatgtac taacaaaaga
    33961 agaacagaca caagctagcc gaaaaactga aacagtccat gaaaaagagt tcggttcctt
    34021 tgcttttcga gcctccactt ctgcagaaga ccctgttgcg ccatatacca tcaactacat
    34081 cgattttggt atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt
    34141 tttagcgctg tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca
    34201 gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca
    34261 gtattatgat tctcctattg atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac
    34321 gatttgtcat caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt
    34381 tgggacatta gaaggggtga tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc
    34441 ttcgcctttt gctcaacatt attttctcac tcatctggat gtagaagata cattgaaaca
    34501 aagcggatta gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca
    34561 ccatctatta gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca
    34621 agacaaatta ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt
    34681 agctgcattg atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga
    34741 ggtcgtttcc ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt
    34801 ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac
    34861 agaaagaatc agcttcaaga aataagaagg ggttttacat aaattgtttc tcaaattttt
    34921 gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt
    34981 gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa agggaacatg gaacacattt
    35041 atcctgtttc cctgttccct ttttgatgta ttcgagcata aataaaatac cttcgttacc
    35101 tactatcttc tgtacttggt aaaccttagt caattgcctt ttttaacgct tgacgaaaag
    35161 acatatgatc tttctccgta aagggttttg gaccctttgt ccttatgcca gcttttctta
    35221 actttccact atgataacgt tgttctagaa gcgtgccgat attcgcttcg tcaaattctt
    35281 ttccagagtg atggaacact cgcacaccct tcggcaatac aagtgaagct aaaccataat
    35341 cttgcgtgat caaaaagtca ttcttcttaa ccataccgac gattttataa tccgcactat
    35401 cagctccgcg gtctacgtag acgatttgga catgattagc gtattctttg gtcgtataat
    35461 ggtccacact ggtcacgata accacgtcaa gaccatagga ttctcctaat tttatgacat
    35521 cttcttttac tggagaacca tctccatcaa tgaatagcct cattcttttt cgccttcttc
    35581 cataaatgat aaacagttac taatccgctc agtaaaagac caacattgat ccattttgcc
    35641 aagataagca gttgtacact ttctatcggc actgattcat ggttcagcgt gatcaataat
    35701 agatggattg gctctcccat ttcttttgct aaaacaaaca aaagcgtcat caataaaaac
    35761 tggtggatca cactcccttt attttgagta gtatacttga caacgatccg gcgatccatt
    35821 ggaatagctg tatcagtttg ataatattcc ttatttagcc agctgcctgt tactacgtgc
    35881 cagaaactaa gcagaaagac acctatcgca aaaccatttg gaatcaagag gaccaataaa
    35941 gaagaactca gtccaagcaa ttgcatagcc aatggagacc atgttaaacg tttcaaaatc
    36001 catggtccga ctatttttgc tgcaatcaag cctgctgcat aaggcaaata cataaaaatc
    36061 gaaagttttt ctaatccgta cagtatgcca acataaaaac tgctaaataa aaaaaggaaa
    36121 ttccctagca ttccatttag aaaagtaaac agatttagcc agcttggcaa cttccattcc
    36181 ttcttctggt gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa accataaata
    36241 gcaagatcaa aaagcctttc atcgaataac aatcgggcat ttctcaaaag aaacaataag
    36301 ccaaagaata tcaaaaataa aacaagctct cgcgtagcta ccagatttct tttgatgtct
    36361 ttgaaatcca gttcgtaacg cggataatga ctaaccgtat ggtaggccat gacgtaaaaa
    36421 agtgtataaa tagtaaagct tgctgcaatc tgagtcggtg caggcaactc aatcgcttga
    36481 tacaggagaa atagccagat agctgcaaat aagtactttt tcccatccat attgataaaa
    36541 ccttgcgtct tttcgtgaaa attgaccgtg gtattagcag gtacaagcca tgctgcactc
    36601 agccctagca aaacacttga aaaataaaat aaaggaaaat aaaatacgcc taatattcca
    36661 agcaatgaac cagcaccacc aatcagcaga gcgatcatca gcaacgtgta actatctaga
    36721 ccaaatcgta gactgttgac taaaaatagt ccagtcatcc gaaaggcata aaacagcgta
    36781 aatggcaaaa tcgtcagcca attatgcgag tctgttagtc ccaaaaataa aagataagga
    36841 ataaaaataa ccgtgttact taagataaag ggcgttgttt gtgtaaaatt acgcccaaat
    36901 cgtttgagtt tttcgaacat gacaatggct ccttaaacag tcttataact aatctgctct
    36961 tcgattttag cattaaaggg gaaataaacc aattttacag aatatattct tgtaaaaaaa
    37021 aatacagaaa gaagcataag cttctttctg catttttacg gtacttgatt actcaagcag
    37081 acaagccctt ctccacaaaa gtcttgcccg acacccctgc atccggcagg tgataaagat
    37141 agaaatcaat tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc agagtccttc
    37201 tcgacccgtc gcataaattt actttaacac agttcctatg tttatgcgag agcttttttc
    37261 attttataat cgaccatttg aaatccttga ccgatatctg aaataagttc ttctgtaatc
    37321 tcaaatcctt catgcttata aacctcgatt gcttgatgat tcccctgatt cactcgcaaa
    37381 aagatctctc tttcctttgt ttgcgcagca agttcttcat accagcgaaa aactgaagaa
    37441 gtcaatcctt gaccacgtac tttatttagc aaatataatt t
//
LOCUS       DAGCWU010000009        37101 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.009, whole genome shotgun sequence.
ACCESSION   DAGCWU010000009 DAGCWU010000000
VERSION     DAGCWU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.009"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(60..884)
                     /locus_tag="MBZ71_000363"
     CDS             complement(60..884)
                     /locus_tag="MBZ71_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBT4428344.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(857..1537)
                     /locus_tag="MBZ71_000364"
     CDS             complement(857..1537)
                     /locus_tag="MBZ71_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBT4428345.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1560..2498)
                     /locus_tag="MBZ71_000365"
     CDS             complement(1560..2498)
                     /locus_tag="MBZ71_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBT4428346.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2743..3606)
                     /locus_tag="MBZ71_000366"
     CDS             complement(2743..3606)
                     /locus_tag="MBZ71_000366"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBT4428347.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3748..6321)
                     /gene="mprF"
                     /locus_tag="MBZ71_000367"
     CDS             complement(3748..6321)
                     /gene="mprF"
                     /locus_tag="MBZ71_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBT4428348.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6462..7268
                     /gene="proC"
                     /locus_tag="MBZ71_000368"
     CDS             6462..7268
                     /gene="proC"
                     /locus_tag="MBZ71_000368"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBT4428349.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7347..8903)
                     /gene="rny"
                     /locus_tag="MBZ71_000369"
     CDS             complement(7347..8903)
                     /gene="rny"
                     /locus_tag="MBZ71_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBT4428350.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9314..10363)
                     /gene="recA"
                     /locus_tag="MBZ71_000370"
     CDS             complement(9314..10363)
                     /gene="recA"
                     /locus_tag="MBZ71_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBT4428351.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10472..11713)
                     /locus_tag="MBZ71_000371"
     CDS             complement(10472..11713)
                     /locus_tag="MBZ71_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBT4428352.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11953..12531)
                     /gene="pgsA"
                     /locus_tag="MBZ71_000372"
     CDS             complement(11953..12531)
                     /gene="pgsA"
                     /locus_tag="MBZ71_000372"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBT4428353.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12740..13663)
                     /locus_tag="MBZ71_000373"
     CDS             complement(12740..13663)
                     /locus_tag="MBZ71_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4428354.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14402..15688)
                     /locus_tag="MBZ71_000374"
     CDS             complement(14402..15688)
                     /locus_tag="MBZ71_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBT4428355.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15681..16949)
                     /locus_tag="MBZ71_000375"
     CDS             complement(15681..16949)
                     /locus_tag="MBZ71_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBT4428356.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17201..17662
                     /gene="mscL"
                     /locus_tag="MBZ71_000376"
     CDS             17201..17662
                     /gene="mscL"
                     /locus_tag="MBZ71_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBT4428357.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17721..18167)
                     /locus_tag="MBZ71_000377"
     CDS             complement(17721..18167)
                     /locus_tag="MBZ71_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4428358.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18260..18505)
                     /locus_tag="MBZ71_000378"
     CDS             complement(18260..18505)
                     /locus_tag="MBZ71_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBT4428359.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18620..19198)
                     /locus_tag="MBZ71_000379"
     CDS             complement(18620..19198)
                     /locus_tag="MBZ71_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4428360.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19275..19805)
                     /locus_tag="MBZ71_000380"
     CDS             complement(19275..19805)
                     /locus_tag="MBZ71_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBT4428361.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19809..20669)
                     /gene="rlmB"
                     /locus_tag="MBZ71_000381"
     CDS             complement(19809..20669)
                     /gene="rlmB"
                     /locus_tag="MBZ71_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBT4428362.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20662..21057)
                     /locus_tag="MBZ71_000382"
     CDS             complement(20662..21057)
                     /locus_tag="MBZ71_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBT4428363.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(21054..22466)
                     /locus_tag="MBZ71_000383"
     CDS             complement(21054..22466)
                     /locus_tag="MBZ71_000383"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBT4428364.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22463..22999)
                     /gene="cysE"
                     /locus_tag="MBZ71_000384"
     CDS             complement(22463..22999)
                     /gene="cysE"
                     /locus_tag="MBZ71_000384"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBT4428365.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23085..23324)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23321..24781)
                     /locus_tag="MBZ71_000385"
     CDS             complement(23321..24781)
                     /locus_tag="MBZ71_000385"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBT4428366.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24951..26108)
                     /locus_tag="MBZ71_000386"
     CDS             complement(24951..26108)
                     /locus_tag="MBZ71_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBT4428367.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26142..27512)
                     /gene="radA"
                     /locus_tag="MBZ71_000387"
     CDS             complement(26142..27512)
                     /gene="radA"
                     /locus_tag="MBZ71_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBT4428368.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27575..28102)
                     /locus_tag="MBZ71_000388"
     CDS             complement(27575..28102)
                     /locus_tag="MBZ71_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBT4428369.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28332..29138
                     /gene="proB"
                     /locus_tag="MBZ71_000389"
     CDS             28332..29138
                     /gene="proB"
                     /locus_tag="MBZ71_000389"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBT4428370.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29135..30382
                     /locus_tag="MBZ71_000390"
     CDS             29135..30382
                     /locus_tag="MBZ71_000390"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBT4428371.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30458..30802
                     /locus_tag="MBZ71_000391"
     CDS             30458..30802
                     /locus_tag="MBZ71_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428372.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30919..31245)
                     /locus_tag="MBZ71_000392"
     CDS             complement(30919..31245)
                     /locus_tag="MBZ71_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428373.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31618..32819)
                     /locus_tag="MBZ71_000393"
                     /pseudo
     CDS             complement(31618..32819)
                     /locus_tag="MBZ71_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            32936..33451
                     /locus_tag="MBZ71_000394"
     CDS             32936..33451
                     /locus_tag="MBZ71_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4428374.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33650..34096)
                     /locus_tag="MBZ71_000395"
     CDS             complement(33650..34096)
                     /locus_tag="MBZ71_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBT4428375.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34143..35621)
                     /gene="msr(C)"
                     /locus_tag="MBZ71_000396"
     CDS             complement(34143..35621)
                     /gene="msr(C)"
                     /locus_tag="MBZ71_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBT4428376.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(35975..36604)
                     /locus_tag="MBZ71_000397"
     CDS             complement(35975..36604)
                     /locus_tag="MBZ71_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBT4428377.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(36588..37052)
                     /locus_tag="MBZ71_000398"
     CDS             complement(36588..37052)
                     /locus_tag="MBZ71_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4428378.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
BASE COUNT        10485 a         8076 c         6577 g        11963 t
ORIGIN      
        1 aaataaaaag aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt
       61 tagttatttt ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac
      121 aaagcaataa agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta
      181 gttagtcctg acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg
      241 aagcttttgc ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa
      301 agtgcaccgg caatcgggat catcacagca atagccacac cagtaatcac attgaacatc
      361 atcgacatca aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga
      421 acatacatcg gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct
      481 aaaaagatga cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata
      541 cttgttgctg aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca
      601 gccattaaaa tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc
      661 aatatcacag ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa
      721 aagaatccta acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat
      781 tgtcttctga tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg
      841 gcaagaaacg ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc
      901 tcaccagtcg gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca
      961 ttagaactga tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc
     1021 tggattcttc atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag
     1081 caaaaatacg cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc
     1141 gatttcgcat atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt
     1201 tttcagagag acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga
     1261 tcaccgtact aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac
     1321 ttaatttttt tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca
     1381 gtaatcctaa cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa
     1441 tgacaaattc acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat
     1501 agtaaaaggt caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact
     1561 taattgatac tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg
     1621 cctgctttga tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact
     1681 cctgtttcat tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc
     1741 actttgatgt tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt
     1801 tcctgatctg gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa
     1861 taagcaaaag cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg
     1921 gagtattttt gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa
     1981 gtttcctttt tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt
     2041 atagctaata ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct
     2101 tcttctgatt cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct
     2161 gcttcgatca tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat
     2221 tccatgtctg aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt
     2281 gcacttggtt cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct
     2341 tcatccccta cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc
     2401 aaatcctctt ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca
     2461 cctatcatta ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac
     2521 aaatcggaat tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc
     2581 aatagaaatc catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg
     2641 atgagttgct gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt
     2701 aggattcctc agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac
     2761 taaataaact tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata
     2821 atgttgacac aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc
     2881 gtacttgatc atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc
     2941 ttctaaactg tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc
     3001 agcaagtccg tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt
     3061 acgtgtagag acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg
     3121 taatacttgg atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta
     3181 aggaacatct gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag
     3241 attccataat tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa
     3301 tccagctgct actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta
     3361 tttatttttt agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa
     3421 tgctttattt gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc
     3481 tgccaagtct acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata
     3541 taatacatcc agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc
     3601 acccattcga aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct
     3661 tatgtctgcc tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt
     3721 cataatccaa acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact
     3781 cggaacattt cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa
     3841 attacttcct ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt
     3901 cttttcataa gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat
     3961 gactttttct tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc
     4021 tagattggct tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata
     4081 gtcagctaat tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt
     4141 cttcgtcatc ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc
     4201 gacaccgcta gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg
     4261 tgcaccttga agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag
     4321 ttgatcgtac caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg
     4381 actcagccga gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt
     4441 gaagtgaaaa cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc
     4501 aagctgatag cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat
     4561 tttaggattg ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc
     4621 aaaaacgact tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg
     4681 aagctgatgg cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt
     4741 ttcgtactca ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa
     4801 agctagcaat gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa
     4861 caaaataaaa tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc
     4921 aattgcgtaa acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca
     4981 agaataaacg aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat
     5041 caagacatat accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc
     5101 aaaagatagt aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct
     5161 agcaaggccg agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa
     5221 tgagaacggc aataatactt gaaatagccg actgattgca gacagattag tgattgttga
     5281 tagaagtacc atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac
     5341 cttttggaaa aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg
     5401 tgtgatgaac aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata
     5461 taatagccaa actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat
     5521 caaaacgtca aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc
     5581 aatcatgaac ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat
     5641 gatcaaaaat accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag
     5701 caagcctttt ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa
     5761 aatgactggt gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact
     5821 ttctgactga ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt
     5881 cagcataaat agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt
     5941 tccataaaaa ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt
     6001 gatcgtgttt gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc
     6061 ttgtttttct aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag
     6121 cagtccagcc agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca
     6181 aatatcttgc caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa
     6241 cacggagccg aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat
     6301 caagtatcgt ttatttttca cattttccca ttcttaactt gtatactttt gtgtcatacc
     6361 ccaatgttat cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga
     6421 tagaaaaaga aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg
     6481 gcacagggaa tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga
     6541 aagaagaggt gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa
     6601 ctggcgtcca tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt
     6661 tagccgtgaa accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga
     6721 aagtcttatt catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg
     6781 aagatcctca ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag
     6841 gcgtttctgc gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga
     6901 tgatttttga agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca
     6961 ttggccttgc tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga
     7021 ctggtgtact gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta
     7081 tgggttcttg tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag
     7141 tttcttctcc tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca
     7201 tcccagcaat catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata
     7261 aaaaataatg atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg
     7321 ccgcggctct tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga
     7381 ataacgatta ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata
     7441 tcacgtacta aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga
     7501 acttcgcgtc cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata
     7561 ttttccagac ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt
     7621 cttgctgctg agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc
     7681 acatctccat gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca
     7741 gctaattccg caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca
     7801 atatcatgga gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct
     7861 aagataccag ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta
     7921 gttctgaatc gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg
     7981 acttcaaacg ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat
     8041 ttctcgacca tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc
     8101 aaggtcatcc gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc
     8161 ggtgtatcat cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga
     8221 ccttctcgtc caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca
     8281 gtcgtttctg aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc
     8341 gctttgcgat ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac
     8401 tcatgattca gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat
     8461 aaaccagcaa ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt
     8521 tctcgctctt caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag
     8581 ttctcgcgtt tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa
     8641 cgattctctt gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa
     8701 cggtatttct gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct
     8761 tctttttttg cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc
     8821 tcgtgacgtg actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata
     8881 gcgaggagaa tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca
     8941 tctatacagc ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg
     9001 tgtacaaaaa acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa
     9061 taaacaaaga aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac
     9121 tagtttcata taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag
     9181 atgaacagat atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt
     9241 tattcaataa aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg
     9301 tttatcataa tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg
     9361 ggacagcgac ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca
     9421 tcatttcagg atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat
     9481 cttctttata agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt
     9541 ctagaagttc tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt
     9601 tgaaaggagg tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat
     9661 ctgtaccttg tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt
     9721 tcaatgcgcg tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac
     9781 ggatttggtt gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac
     9841 gcaatgcttg agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt
     9901 cgatttctgc tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc
     9961 cacttgatac taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa
    10021 gaagtagttc atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct
    10081 cagcatcaat gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg
    10141 caactgttgt tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt
    10201 aaccgccgac acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt
    10261 gttggtcgat tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt
    10321 ttttcagtgc agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta
    10381 tatttacaaa tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag
    10441 caagaaaaaa ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa
    10501 gatcaaatcc ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat
    10561 atttttccga aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt
    10621 gaccttctaa cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg
    10681 ctatctcacg tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt
    10741 ctgctaatag ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa
    10801 cgaatccgcc tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg
    10861 cgccggctgt caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca
    10921 ctacgacttt tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt
    10981 cttggatttt ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt
    11041 tcactgtcag tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga
    11101 tctgtgtctc aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa
    11161 agcgcaaaac tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac
    11221 gtacttgttc cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg
    11281 catgtgtatc tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa
    11341 ccccgccttc gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg
    11401 tttcaaaata tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga
    11461 ggtgagccgc agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata
    11521 agagtatgag ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat
    11581 tatctcctac aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca
    11641 agaaagtggc attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta
    11701 tttctgcttt cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt
    11761 tatttaaaaa gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat
    11821 aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa
    11881 gctgtttttt gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc
    11941 actaaacgat acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac
    12001 acctgaatag atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc
    12061 gatcaggctg aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc
    12121 cgtcttgact tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa
    12181 acgcaagcct gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc
    12241 ctttccttgg tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg
    12301 gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa
    12361 ccagtctgtg atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag
    12421 tgtggtgtct cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa
    12481 aatcgggatc atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac
    12541 tcctttcttc cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt
    12601 ccaaatgaat ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag
    12661 aaggaccgcg acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg
    12721 cgtaaactaa tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac
    12781 tctgattgtt ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat
    12841 tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct
    12901 gtgtttcatt tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta
    12961 accatacacg attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat
    13021 ttactgcgat ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac
    13081 ttgattttgt tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg
    13141 aagaggactc ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg
    13201 gatcagcttg acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa
    13261 ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct
    13321 gatgcattgc ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc
    13381 ctgtcaattt gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag
    13441 cttcagcgta ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat
    13501 catcattgtc gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat
    13561 ctaaagatat ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag
    13621 ccactcgttc atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa
    13681 gacgttatgt ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac
    13741 attgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg
    13801 ttttttgaac ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta
    13861 aacggtgttc aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga
    13921 tatttttccg tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct
    13981 taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa
    14041 gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
    14101 ttggtacacg gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt
    14161 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
    14221 ctctttgatt acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt
    14281 tgttttattt tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg
    14341 gaggaggttc ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg
    14401 tttattttgg tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag
    14461 ctgccagtac gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc
    14521 gatcgccaaa taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt
    14581 ggaaatattg tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg
    14641 gatcatcagc gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt
    14701 ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa
    14761 agctgtcatc gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta
    14821 aaatcatttg gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt
    14881 gcggcaatgt gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt
    14941 ccaatgcact ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt
    15001 gctttggagg gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag
    15061 gctccatctt accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc
    15121 acgtatacaa gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga
    15181 tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag
    15241 ggtcgtcttc atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt
    15301 cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct
    15361 gatccgtcgt agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag
    15421 ctccttgttt cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt
    15481 ctaaaaaatg ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa
    15541 attcgttatc gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat
    15601 ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat
    15661 tgatttgctc atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc
    15721 tgcaattcga tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt
    15781 ttcaaccatt cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct
    15841 tcgattgccg cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt
    15901 gtctgagaca gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga
    15961 atcagatgaa gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat
    16021 cctcggaacg tatccacgct actagaggca taataagcta gactttcttt ctctctgaca
    16081 ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca
    16141 aaacgttctg gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga
    16201 acgattggct cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac
    16261 agatctggat aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct
    16321 ttgtccggat caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct
    16381 tgatagtagg acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac
    16441 cctgggatct tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca
    16501 aaagtttgtt tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag
    16561 tcaaacgtca actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt
    16621 aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa
    16681 ctgatccctg cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt
    16741 gcgccataaa gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta
    16801 tttgtttcca acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc
    16861 gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga
    16921 ttcacgcctt ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc
    16981 cgcttactct tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat
    17041 aagttcaggc aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat
    17101 gttttttatc aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt
    17161 tcgatatact aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga
    17221 gttcatcatg cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt
    17281 tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt
    17341 cgcaatgatc acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc
    17401 gacaaaaggc gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat
    17461 cactggtttt gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc
    17521 caaagaagag caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat
    17581 acgcgatctt cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa
    17641 tctttccgat acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc
    17701 ggctttcttt ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga
    17761 atatcatctt ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct
    17821 tgtaggtatt cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg
    17881 ttaatcgttt cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca
    17941 aggacacgtc caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc
    18001 ttcccatcag tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct
    18061 tgataaggac actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa
    18121 aactctgctg ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc
    18181 cctgcttttt tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa
    18241 tctcaggctc gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag
    18301 taagtacatc tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat
    18361 caacaacgaa tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct
    18421 ttcttcctgt ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct
    18481 tgattgaagc taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt
    18541 tttaaaattt tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc
    18601 tcattaagat ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga
    18661 gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca
    18721 tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa
    18781 actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt
    18841 cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg
    18901 agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga
    18961 tttgattgct tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt
    19021 aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat
    19081 aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca
    19141 gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa
    19201 aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg
    19261 attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt
    19321 tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta
    19381 ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa
    19441 gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact
    19501 gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc
    19561 gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg
    19621 cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa
    19681 tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc
    19741 tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt
    19801 ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca
    19861 acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt
    19921 tcttttttca atcctggact cattcctttg ccttcattac caatgactaa agcaattgat
    19981 cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat
    20041 cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca
    20101 tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt
    20161 tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa
    20221 ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt
    20281 gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact
    20341 ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact
    20401 ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct
    20461 tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc
    20521 ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt
    20581 ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat
    20641 tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg
    20701 gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc
    20761 gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt
    20821 agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag
    20881 catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg
    20941 cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta
    21001 aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct
    21061 tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac
    21121 aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct
    21181 tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag
    21241 atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc
    21301 ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt
    21361 gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa
    21421 gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa
    21481 ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca
    21541 ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga
    21601 tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt
    21661 tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc
    21721 cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc
    21781 ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca
    21841 atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt
    21901 gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta
    21961 cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt
    22021 tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata gcctttttca
    22081 accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt
    22141 gtagcaggct cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca
    22201 gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca
    22261 tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt
    22321 gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt
    22381 ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg
    22441 gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa
    22501 cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg
    22561 cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg
    22621 cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc
    22681 cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa
    22741 tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga
    22801 tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc
    22861 ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg
    22921 tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg
    22981 ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca
    23041 tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc
    23101 gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac
    23161 gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga
    23221 ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc
    23281 ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta
    23341 aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat
    23401 gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag
    23461 tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact acttccattt
    23521 gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt
    23581 cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga
    23641 tttctgcagc atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga
    23701 ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca
    23761 tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt
    23821 ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca
    23881 attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa
    23941 agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga
    24001 ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat
    24061 gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat
    24121 tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg
    24181 caacagcaaa attataagtt ggcataccat cgcgtttttg tatgacaaag tcgccaccga
    24241 tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat
    24301 tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt
    24361 cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt
    24421 gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc
    24481 tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag
    24541 ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt
    24601 tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac
    24661 gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg
    24721 catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca
    24781 ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg
    24841 cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa
    24901 tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt ttattttgat
    24961 tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt
    25021 ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata
    25081 cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag
    25141 gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca
    25201 gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt
    25261 tggaattcag agactttatt caagttatag tcattggtta caatgacacc atctaaaagc
    25321 ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac
    25381 atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga
    25441 ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca
    25501 aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga
    25561 ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt
    25621 cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt
    25681 gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag
    25741 cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg
    25801 taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg
    25861 aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct
    25921 gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg
    25981 ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag
    26041 ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt
    26101 ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc
    26161 aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta
    26221 cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact
    26281 cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa
    26341 cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct
    26401 aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga
    26461 ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg
    26521 aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct
    26581 tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt
    26641 gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca
    26701 tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct
    26761 cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata
    26821 tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa
    26881 atggctatcc catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg
    26941 ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg
    27001 ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca
    27061 gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc
    27121 tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga
    27181 gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata
    27241 gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc
    27301 cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc
    27361 aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc
    27421 cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg
    27481 caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg
    27541 agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc
    27601 cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt
    27661 ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga
    27721 acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc
    27781 cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa
    27841 attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt
    27901 tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc
    27961 agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag
    28021 cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa
    28081 cttcaaatcc tctttttctc accggttttc ctccctaaat aaactactcc tattctaaca
    28141 tatccgaggc tgtaaaagac tttctttaca aattcttcaa gaaattctgc tatcttaaag
    28201 taaaccatta attcgttctt tttccaaacg actgcaagtt gtacaaaaaa actatttttt
    28261 acatgacttt actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg gatatttgat
    28321 tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa
    28381 caagtacatt gatttatccc aatggaaata tcaatcttgc ggcaatcgat cagttagctt
    28441 ttgtcctttc tgatcttaga aatcggggaa aggaagtgat cttagtctca tcaggagctg
    28501 taggcgttgg tatgcataaa ttacatttgt cagaacgtcc gactttgatt cctgaacagc
    28561 aagctgcggc ggctgttggt caagccgaac tgatgaatat ctataatcaa agattcagtg
    28621 tatataatca acagacggca cagatcctcg tgacaagaga cgtggtagat tatcctgaaa
    28681 gtcgtaaaaa tgtgaggaat acattggaac agcttttgat tatggacacg atccctatcg
    28741 tgaacgaaaa cgatactgta gcagtggatg aattagatca tctgacaaaa tttggcgata
    28801 atgatcagct gtcagccatc atggcaaaac ttgtcgcggc agaccttttg atcgtccttt
    28861 ctgatatcga cgggttttat tcagataatc cgactacaaa tcccgaggct cagatgtatc
    28921 gctatatcga taaaatcgat gaaaagattc tggcacaagc aggtggtgct ggtagcacat
    28981 ttggaacagg cggtatgcag agtaaattaa aagccgcttc taggatattt gaagcaaaca
    29041 gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa
    29101 aagaagtagg tactttattt aaggaggact tgaaatgact gacttgatgc aattaggaca
    29161 acaggcaaaa gatagcgcgc aacaactagc acttatgcct acaaaccgca aaaatgagct
    29221 acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca
    29281 taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca
    29341 gctgacacct gagcgaatca atgccatggc tacagagatc gaaaaaattg cttcgttaga
    29401 cgatcctatt ggaaaagtag atgagatgtg gacgaacacc gacggattga gaatcggcaa
    29461 aaagcgcgtg ccactaggcg tgaccggcat catttacgaa tctcgtccaa atgtcacgac
    29521 agatgcagct agtctagcat tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga
    29581 agcattcttc tcgaatcaat tattagtcca attattgcag caagtactgg tcagtgctgg
    29641 cgaatctcct tatgccatcc agtttgtcga tgatacttct catgaaactg caggacagtt
    29701 gatgcgattg acagaatact tagatgtgct gattcctcga ggcggagcaa aattaatcca
    29761 acgagtcaaa gaacaggcaa cggtacctat cattgaaaca ggtacaggaa ataatcacgt
    29821 atatatcgat aaagaagcac agttatctat ggcagtgaac atcatcgtca atgcaaaagc
    29881 ttcccgtcca tctgtctgta acgcggcaga aacattgctg atccattctg agatcgcacc
    29941 ttttttcttg ccagctattg aaaaagaact ggtagaacac ggggtctctt tacgtgcgga
    30001 tgctcgtgcg ttagaatatc tagagacggc tgctttagca gaagacacag attgggatac
    30061 tgaatatcta gattatatcc ttgctgtcaa agtggtcgat tctttggatg aagcaattgc
    30121 acatatcaat cggcataata ctaaacattc agaaacgatc gtgacagata gttatgcggc
    30181 tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg cctccactcg
    30241 ttttacagac ggatcggtat ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt
    30301 gcatgcccgt ggtccaatgg ggttaaatga attaacttcc acaaaatata tcatttatgg
    30361 cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt attcccgcct tttgcctttt
    30421 ttctgtaaag gttctatcca actagaaagg tgtcaatatg tttgaaattt taatgatttt
    30481 ttttatttac cttatttctc tgaatatcgc tgcttttctg ggagtcggta ttttatccct
    30541 tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt
    30601 tgacaaaatc gggcaaaaag ggctggttac acgcttgcac attagttaca tggttgcatt
    30661 atgcttatta gcgacaatca attactattc ttttttcgat cattcaatcg cttatacgat
    30721 tacgctactg attgctggga tttttcactt atcttataaa tatcagctga ataaaaacca
    30781 cttgaatcgg actttccgct aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg
    30841 gcaaaaaact gccggacttt ttagaggctg tgacaaagat tttgtcacag cctctttttc
    30901 catataaacg gcgttcgcct accagctgcc tggtaaatct gtttcctctt cgttaagaag
    30961 cgaaaatccg tcaaatgttt cgccatcata ataacgaagc agctcatcca tggacaagtt
    31021 catcgttttt tctttttctt cttccgctga ttcagccagt agctgttccc gatacacctg
    31081 atacatacca gctatttttt gctgcatcgc ttttctagtt tcaccttccg cattgattat
    31141 tggcaattct ttgatatagc cacgataaat caaccgatca tctgaaaatt caatgggtaa
    31201 ttcaacgagt gtcaacccga cttcctgaaa atattcttcc agcatctatt atccccccat
    31261 tctttttctt aaatgaattt tagcttacct actgaaaatt ttccagtttc attattttga
    31321 gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt
    31381 cttctgccaa ctcctcaatc ataagtcaca atattcaaca aacatgagaa actgtttttt
    31441 gaaattgttc cttgtgattc agagttaaac gacagtctca caacctcttg gtaaacggcg
    31501 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt
    31561 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tgatttctta
    31621 ttcttcttca tttgtagcaa aataattacg gtgataaatg atacaacata cactgataag
    31681 aacaatggca cctgcaaaat aaaacgcata gctttcacca atatgttcac caatcacacc
    31741 tgcaataaat ggtgctaaag ctccaccgaa aaagcggaca aaactatagc tggacgaagc
    31801 aacgtttcgt tgcagacctg gtatttccat agcaatcgtc gtcaacagtg tattgataat
    31861 tccttggaaa aatcctgcta acacaatgct gatcggtaca aggactggca tagaagttcc
    31921 agctcctaat aacagcaaac aaataatgaa taagacaaat gctgctaaaa ttgtccgata
    31981 agtcgtaaac ttccgctcaa gagccggtgc aacgaaaatc gaagcaatag ccagaagcac
    32041 accccagcca aaaaatacaa aaccgacttc taattcagaa tagccaatca ataaaaatgg
    32101 cgcatacgca agtaaagtaa agaatccgaa attatataac aatgcgatta gtccaactga
    32161 acgaagttta tgattgctta acgctttgac accttcccaa aggcctactt ttctctttgg
    32221 tttctcagtt tcaggtaaaa gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc
    32281 taccccaaaa aatggataac gccatgaacc actgcccaag accccaccga ctagagggcc
    32341 aacagccatt ccgcagccaa gtgctgcttc atacatcatg attgcttttt ctgtgttacc
    32401 aactaaaata ctgacgatcg ttgctaaagc agtagagaca aataaagcat tcccgacacc
    32461 ccagccggca cgtaacccaa tcagcattcc gatattccca gataatccgc caagaccagc
    32521 gaatacaacg atgataaaca atcctgtcat caatgttttt tcaaaccgat ccgactagat
    32581 aagaaacctg taaatagcat caccacacca gtgactaaca tgtaactagt aaataacagg
    32641 gtcgtttcag ctggcgtagc gtttaactgt gcagcaatcg tttttaagat tgggtctact
    32701 agacccactc ccataaaagc gatcatacag gtagctgcta caactagcgc tgaacttttc
    32761 ccctttgttt cttcatttgt ttgttcttgt atatctattt gattctccaa tatttccatc
    32821 cctttctctc tattagcatc atacatgatt tattttacat gtaaatatgc acatgtaaaa
    32881 cgtggaaact ttactgtgat ataataaaga aaataagacg agaggtagga tttttatgaa
    32941 tactttagcc tatatattat tatgcgtgct tttatccaaa ccacgttctg ggtatgaatt
    33001 gaagcagctg atcacaattt tttgggaagc ccatcacagt cagatctata caacgcttgc
    33061 caagttagac cagcagggtt atgtgaccgt tttggacaaa gacgagcact ctcaaaaaaa
    33121 gatctaccac ttgactgatg aaggacgtgc tcttgtagag gaatggatca aagaggaaac
    33181 tcccgctcct acgcaaaaag atgaatttct agcaaaaatc tatgcttttt cgacgctcga
    33241 taaaacaaca gctactggac tgctattcac tagagaacaa cagctgaaca aaatcatgca
    33301 aaaaaatcaa aaaaaattga atgacctctc gctagacaag aaacaagaat ttggacgtta
    33361 tgttgtgatc cagcgcagaa ttatgctttg tcagcaggaa ctccaatggt gtcagcaagt
    33421 aagagaacaa atgcagcagt tttttgagta agggataaac agaacctttc tagaaatatc
    33481 tattgaagag caaattatct tcaataaaag agcaatacag ttggaaaaga aaaagcgatc
    33541 cgaacgacag gccaaaacct atcgttcgga tcactatttt tctagaactg cattcaagag
    33601 actatgacaa aaagcatgcc aaggcctttt ttcaaataac agtgttccat cattcattac
    33661 tttgaattgc ttttagcaca cttcttgaaa gcgggccaac gatgatcaac tgcaacggta
    33721 aagcggcaat cacattcatt ttccaagcat tcatataagc agagaataat tgatcaggta
    33781 ttccttgttc catgatcaca ccgaataaag acatacatgt caccattcct aaaaccatca
    33841 agcatgaaat agttaagatc atttggacct tgctttctgg atttactggt agtttaaagg
    33901 caattttttt cgccagcaca ccgatgatga agacatccaa aatgaaagcc accacgaatc
    33961 caggcactag accaatcaac aaacttgata aatgaagatc ttgatgcagc cataaattat
    34021 acgcgctcat ccccaagacc atcaaaaaac acatcattgt tgtaaaaatt attccttctt
    34081 ttttatttgt aggcaatctg taccactcct ttaaatttgt cacgaaaatc atcgtagcac
    34141 atttaaaaat ttctcgtaag tacttttttt attttctgcc cttttatttc ataaacttca
    34201 tcagccgtac gctcgacaaa gtaagaatca tgagaagtaa acaaaatcgt tcccggatat
    34261 atttgcatta gcttctctaa agcttcgatc gttgccatat caataaaatt agtcggttca
    34321 tctaacagca acacattact tggcttagta aaaagcaaag caatcgtcag acgggtcgct
    34381 tctcccccac ttaatgtaca taacggtttc gtcaaagttt cctgagcaaa ccctaagtga
    34441 ttcagcaaac tacggacaag cgattctgaa aactctgttt gtctcatgac aaaacgaatg
    34501 atggattctt cagatgtcat ttgataaccc ttttgctgat atacttgaaa gcttactttc
    34561 ggagagagaa ggattccttc tccttgtttg cggatatgtt ctaataaggt tgtcttacct
    34621 gaaccatttt caccgacaat cgcgattttt ttacctaaag aaaactgaaa atcaccattt
    34681 accagtaatg ttctcccact tctttccaat tggacatttt gtcccatgat gggatagcgg
    34741 ctgtggagtt caagagattt tggtactgga aaacggattt gtttcatctg ctgcggcttg
    34801 gttgtttctc ctattttttg gagtcttctc tctaatgttt tcgcattctt ttggatggct
    34861 ttttgtacac tgtctttttg tttagaggaa gacaaccgat ccggtctgat ttgttgcttt
    34921 ttctttttac tggacacttt ttccaacttt tccgcttgtt ttttcttttc ttggatcgat
    34981 ttttccaatc gttttttctc tttcatgatc gactctgctt ctcgtagctg agtctttttc
    35041 tccaattcct tttgagtgag ataggcacta taattccccg aaaactctcg gatttctccg
    35101 tcttttactt cccagatttt cgatgctagc tcatctagaa aatagcgatc atgactaaca
    35161 aaaaccagcg taccataata ataacgaagc tcttcaatca gtcgatcaat actttttcta
    35221 tctagatggg ttgttggctc gtctagaagc atcccttgtt cataagaaga aagaagacgt
    35281 atcaactcga tctttcgttc ttcccctccg ctccatttcc gctctgaagt ttcttccagt
    35341 cttaactgga aatgactggc taattccaaa tttaggtcct ttgcttcagc gagataggtt
    35401 aattgaggca ggtagttgaa ggtgatctct gtttggattt tcccatgatc gggaaaaagt
    35461 tcaccggcta ttagtttgag caaagtcgat ttaccgacac cattttctcc tatgatccct
    35521 atacgatctt gctgatagac acgaagagaa tcaatagata gttctagctg gttgccaaag
    35581 ctgacttgta gatttgttat atttactgct aaattttcca tatgttttac actcctattt
    35641 catgtaaaac aacaattatc gatactaaaa aagagcagaa aaatctgttt gcaaaaaaac
    35701 tgcatcacat cttttcctac tctgaacgaa ataactatga ctaaatcacc aaatctgtca
    35761 tagctttcac ctattctact gtcgttcgaa aaaatggaca tactccatcc atgaaattgt
    35821 tgtttataca tgtcttttga atttatttag ttgttattca aaagttcgaa acgtaatttc
    35881 atcgatgcag tcatttttcc gacctccttt tttattaggt taccacacac gattcaaaaa
    35941 aagcaatagc tactgaatag taactgaaaa ccttttattc gaacgtagtg atcttttctg
    36001 gtgctaaacg aacagactcg taaccttctt ctaccgcttt tcctattgat aaaataataa
    36061 ctggaacata acggtcttta tccaaaccaa atgcctctgc caactgatct gcttcgaatc
    36121 caccaattgg atttgtttca tagccatgat cacgcgcaac taacatgaat tgcatagccg
    36181 ctaagctaga atcgactttg actacatcgt tcatttcttc tcttgaaaaa ctcttgtaat
    36241 aaggaatgat cgcagctaat tgttgatctc tgacttcttt tggcatcttc cctgattcgt
    36301 aagcttggtt atagatttct tcgccatatt cgtaacattg catatcgcca aaaatcatga
    36361 ccattgcaga agaagtatca ttttgtctcg tattgaaacg gatcaaagga cgcaagattt
    36421 cttttgcttc ctcgctttca gcgaccacaa agcgccatgg ttgcatattg acagaagaag
    36481 gtgcagtcgt cgcttctttg atcatttcca gcatttcttc gtgtgggatt ttgtatgttt
    36541 catcatatac gcgaacggat cttctaccag tcataattgt tgaaaaatca tttttttgtt
    36601 ccattattat taacctcttt cgtttctgtt gttatattgc aaagttgggt attcaattta
    36661 tctaaaagtg ttagaaattg tttcatttct acatcgctga aagccgggga tagcacggaa
    36721 cctgtatctt tatggtccat ttcacaatgc tcgattttct tctgggcttt ttctgttagc
    36781 gctacataca cttcccggtt gttctgagga tttctctgac gcgaaacata gccatttgct
    36841 tccaatacct tcagatgacg agtaactgct ccttgatcaa tctgcaactt ttcttgcaaa
    36901 ctgctttgta gcaatggatt ttcttctttc agtctcatca tgatctcgta tctagtaaga
    36961 cttataccga tattttcttc aaagatctgg acgatttttt gatcggctaa tttgatttga
    37021 tataaaaggt gactaatctc ttctaaagac ataatttcct cctttctaat aattgactcg
    37081 tcaacgattg acttgtcaat a
//
LOCUS       DAGCWU010000010        35910 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.010, whole genome shotgun sequence.
ACCESSION   DAGCWU010000010 DAGCWU010000000
VERSION     DAGCWU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.010"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            75..422
                     /locus_tag="MBZ71_000399"
     CDS             75..422
                     /locus_tag="MBZ71_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428379.1"
                     /translation="MDHLYIKYIHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFS
                     YAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYIIVLIF
                     LSFIVWMFYLVKK"
     gene            517..1401
                     /locus_tag="MBZ71_000400"
     CDS             517..1401
                     /locus_tag="MBZ71_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBT4428380.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1625..4792
                     /locus_tag="MBZ71_000401"
     CDS             1625..4792
                     /locus_tag="MBZ71_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428381.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4812..5357
                     /locus_tag="MBZ71_000402"
     CDS             4812..5357
                     /locus_tag="MBZ71_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBT4428382.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5385..5840
                     /locus_tag="MBZ71_000403"
     CDS             5385..5840
                     /locus_tag="MBZ71_000403"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBT4428383.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5837..6595
                     /locus_tag="MBZ71_000404"
     CDS             5837..6595
                     /locus_tag="MBZ71_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBT4428384.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            7096..7284
                     /locus_tag="MBZ71_000405"
                     /pseudo
     CDS             7096..7284
                     /locus_tag="MBZ71_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7330..8295
                     /locus_tag="MBZ71_000406"
     CDS             7330..8295
                     /locus_tag="MBZ71_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4428385.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            8317..8529
                     /locus_tag="MBZ71_000407"
     CDS             8317..8529
                     /locus_tag="MBZ71_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBT4428386.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            8604..9080
                     /locus_tag="MBZ71_000408"
     CDS             8604..9080
                     /locus_tag="MBZ71_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBT4428387.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            9077..10885
                     /locus_tag="MBZ71_000409"
     CDS             9077..10885
                     /locus_tag="MBZ71_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBT4428388.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            10962..11177
                     /locus_tag="MBZ71_000410"
     CDS             10962..11177
                     /locus_tag="MBZ71_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBT4428389.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            11269..12132
                     /locus_tag="MBZ71_000411"
     CDS             11269..12132
                     /locus_tag="MBZ71_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428390.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            12169..12705
                     /locus_tag="MBZ71_000412"
     CDS             12169..12705
                     /locus_tag="MBZ71_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBT4428391.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            12719..13141
                     /locus_tag="MBZ71_000413"
     CDS             12719..13141
                     /locus_tag="MBZ71_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBT4428392.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            13071..15470
                     /locus_tag="MBZ71_000414"
     CDS             13071..15470
                     /locus_tag="MBZ71_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBT4428393.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            15502..17493
                     /locus_tag="MBZ71_000415"
     CDS             15502..17493
                     /locus_tag="MBZ71_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4428394.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            17516..17767
                     /locus_tag="MBZ71_000416"
     CDS             17516..17767
                     /locus_tag="MBZ71_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBT4428395.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            17757..18986
                     /locus_tag="MBZ71_000417"
     CDS             17757..18986
                     /locus_tag="MBZ71_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBT4428396.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            18983..21064
                     /locus_tag="MBZ71_000418"
     CDS             18983..21064
                     /locus_tag="MBZ71_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBT4428397.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            21213..25133
                     /locus_tag="MBZ71_000419"
     CDS             21213..25133
                     /locus_tag="MBZ71_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBT4428398.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            25134..26078
                     /locus_tag="MBZ71_000420"
     CDS             25134..26078
                     /locus_tag="MBZ71_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428399.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRNQLSDSKQAASPKKAAARTKKNELEV"
     gene            26082..26285
                     /locus_tag="MBZ71_000421"
     CDS             26082..26285
                     /locus_tag="MBZ71_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBT4428400.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(26585..26821)
                     /locus_tag="MBZ71_000422"
     CDS             complement(26585..26821)
                     /locus_tag="MBZ71_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4428401.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(27004..27390)
                     /locus_tag="MBZ71_000423"
     CDS             complement(27004..27390)
                     /locus_tag="MBZ71_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBT4428402.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(27372..28700)
                     /locus_tag="MBZ71_000424"
     CDS             complement(27372..28700)
                     /locus_tag="MBZ71_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4428403.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(28661..28990)
                     /gene="mobC"
                     /locus_tag="MBZ71_000425"
     CDS             complement(28661..28990)
                     /gene="mobC"
                     /locus_tag="MBZ71_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBT4428404.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(29248..29619)
                     /locus_tag="MBZ71_000426"
     CDS             complement(29248..29619)
                     /locus_tag="MBZ71_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4428405.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            29764..29949
                     /locus_tag="MBZ71_000427"
     CDS             29764..29949
                     /locus_tag="MBZ71_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4428406.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            30107..30274
                     /locus_tag="MBZ71_000428"
     CDS             30107..30274
                     /locus_tag="MBZ71_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428407.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            30892..31551
                     /gene="vanR"
                     /locus_tag="MBZ71_000429"
     CDS             30892..31551
                     /gene="vanR"
                     /locus_tag="MBZ71_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBT4428408.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            31554..32897
                     /gene="vanS"
                     /locus_tag="MBZ71_000430"
     CDS             31554..32897
                     /gene="vanS"
                     /locus_tag="MBZ71_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBT4428409.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            33068..33874
                     /gene="vanY"
                     /locus_tag="MBZ71_000431"
     CDS             33068..33874
                     /gene="vanY"
                     /locus_tag="MBZ71_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBT4428410.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            33887..34723
                     /gene="vanW"
                     /locus_tag="MBZ71_000432"
                     /pseudo
     CDS             33887..34723
                     /gene="vanW"
                     /locus_tag="MBZ71_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
     gene            complement(34835..>35910)
                     /locus_tag="MBZ71_000433"
     CDS             complement(34835..>35910)
                     /locus_tag="MBZ71_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBT4428411.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         9610 a         8731 c         9334 g         8235 t
ORIGIN      
        1 aatttctttt atttctagaa gttattcacg tatacggatt tgtttttctc acaaataaaa
       61 aggcaggaag tacaatggat catttgtata taaaatatat tcatcaattt tccgttttgg
      121 tgttaggcac actaattata gcggggattg tcgcatttat tattcaaggc cgtgacatat
      181 taacagtaca tcaagagttt ttcagttatg catttggtat tcctctgatc gctataaggt
      241 ctgataacaa aaagtggaag aaacgctatt acattctctt gctatttttg tgtttttatc
      301 tgccacctaa gatttttact ttattaggta tacaatgcct atcattttct tttatttcct
      361 atatcattgt tttaattttc ctcagtttca tagtatggat gttttatctt gtcaaaaaat
      421 aaatgaatgt ttcatgaaca gtacttcatc acagcctaat ttttttcttt atgctatcgt
      481 tatattaaag aaatcaatca gaagggaaag aaagtgatga aaaaagtttt attagtggta
      541 aaccccagtt caggcggtga acaagcaaag gaatttgagc aattagcgat agcaaaattg
      601 gaatcggtgt ttgatgaagt ggtcgtcttg catacaaaaa aagcagggga tgcaaaaaac
      661 tttactcgcg aagcggctac ggaagggtat catagtgtgt ttgtcatggg cggagatgga
      721 acagtcaacg aaggaatcag tggaatcgct gaacaagaac atcggccaaa tttcggattt
      781 ttcccattgg gtacggtgaa tgacttggct cgagcattag ggataccgct agaacctgag
      841 gaagcgatca atcatttttc gattgaatca gtcaagccat tggatattgg aaaaataaat
      901 gacgactatt ttatgaatgt cgtcgcaatt ggctctatcc ctgaatcaat caatgatgta
      961 gatgcggaga agaagacgaa atttggaaaa ctagcttatt tcatgtcagg aatcaaacag
     1021 ttagcttcca cacaatccta ctcgtttcac gtagaagttg atggaaagaa ggaagaaatt
     1081 gagagcagta ccttgctgat aggactaact aactcagtcg gcggttttga aacattactt
     1141 ccaaatgcaa aagtggatga tggtaagctc catttggttt atttaaaaga ttcttctttg
     1201 atcgatacgc ttaaaacatt gccagaccta ttaaaaggag tagatgaatc tacgaaaaat
     1261 ctagcctacc ttacttgtga gcaattagtt gtgtcattag ctgatgagga agaattgacg
     1321 acgaatgttg atggagatga aggggataaa ttacctgtga caatcaaagt attgccctca
     1381 catttacagg tttattgtta aaatggaggt tgtgaccgag gagttggcgg gagaacaccg
     1441 cttgttcggg ataagggact gtgacaagac ttttgtcaca gccttttttt tgtacaaaaa
     1501 agaaatttac acctaataga aaaatttttc cataaaaata catattttaa atatgaaagt
     1561 acaattggaa atagattaaa acgataaaat aaaattgtca aaacgtcaga gaggatggtg
     1621 gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt ttgtttgtct tccctctctt
     1681 gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac gaatcaacgc aatcagagaa
     1741 tcaaagtttt gacaagacta ctttttcaga agaatcgatt acttctgaaa ctacagaatc
     1801 tcaaagtgag gcttctacta ataaaaggca agaagaaaat aagacaagtg agacctcaga
     1861 agtagctgaa caaacgtctg atagcagtga agcggcactg gcaagagctg tttcagcgga
     1921 tgggtacagc gaagctgcca caacagaaga actggcgcag cttctcgcag acgaatcagt
     1981 aactaagatc cgactgattc agcctttgac tttagacagg gaattagaga tcaaacgaga
     2041 tatcgttatt gattttggtg gattcgctca caatttcggc acacaccata tttatatcaa
     2101 tgagatacct cagtcgatcg agttccagaa ttttaaagga acagctgcac atccaggtgg
     2161 attgacacca aatatcgatg gaaatgccat tatcattgct tatatgaccg atttttgggg
     2221 aagcagatat cattttacgg gagaaataaa atttaccggg acacttgatt tgtatgatgg
     2281 gtccaaatta ggactgattt acgctcctcg tgcaactgtc acattagatg gtgtttccgg
     2341 tgtgttggat gtccaaccag tgaaggaagg actgaatgcc gctccgggta aagcctattt
     2401 ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa ctttatggac cgtatctagg
     2461 gaaattctac ggttttctag attcaggaga tgagactggt agtggaaaaa cagcgccagg
     2521 aatccatatc ttaagcggtt caaaggtctc tcttgattat gatcgggcag atggcgttag
     2581 cgatgaagga gaggcagtcg atacgctacc tggtaatgtc atgtttgaag tcagtggttc
     2641 gggaagcgaa ttttcggtaa atacaaagat caatattacc aatgacaaca atcgcggaat
     2701 catccaaatg agaggttcag gatcgaaagt cactgtatcg aatggtggga aaatcaccat
     2761 caatacagca actacgggcg gatttcgctt gcaaggagat tctagtcaaa tccatgtatc
     2821 aagtggtgga caaatctcgg tcaaaatggc tggagatgac gatcaacccg ggaacaatgg
     2881 gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa ggagccggat cagttatcga
     2941 catcgataag cagagtgggc gttccagcgc agttcgtttt gagaatggta cgcaaaagct
     3001 gacagtaacc aatggaggct cattgagggt aagaaataca ggtgatggca aatcttacgt
     3061 gaacagactc aatcaaggaa atcaagcgat ccaattttat gacgcttctg gttttctaga
     3121 aagtccaggt tcagctgatt ttacagtcac tggagaaaaa tcaaatatcg atatacgcgc
     3181 agattccgga gcaactgtag atacaactgg agatattgat ttaaatttta cggcaggtga
     3241 aaaaacttat ttagtcatgg tcgggaaaac aggggaagat aagaccggca tcatcagtgg
     3301 ggatgtgttg aatgtggact tagaatctcc tacctatttt gatttccaaa acaaacggat
     3361 tggaagcgag accaatggca taggcggctg ggtattccaa ggaaatggag attcttcttt
     3421 aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca ggcgcaacat tcgataactt
     3481 tgaaaaagac ccggattatt tttctgatat gacagactta tcgatcaatg ggaccgactt
     3541 aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat gaatttgctc agacaggtgc
     3601 cggaatgaaa aattataatc gaatcagtgc caataatcag ctcccgatga ttgaatcctt
     3661 aagagtgcca acgaatgcag ataaaaaaat ctatgggcat gtcgttgttc ccgaaggtgt
     3721 cgaggcaaag ccaagagatg cttggacagg cgaagtagaa gtgactttac gtattactta
     3781 cgcagatacg acgaaatcat ctgtagaaat tactgctctc acgcaaggac aaacagatga
     3841 aaatgaaggg gcaggatata acccatgggg agaaggcgaa caaggcggct tgttcgaagt
     3901 tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa gtgcaagttg ttgctgctaa
     3961 aaaagtaaga ggtggccaag aagtgacaga actggactct gccaaaacga cagtcgatgt
     4021 gattcctcct gaacctgcta atttaagtac cacattgatc aatgcagctt ctcgcacgat
     4081 cacaggtaca ggtgaagcag gagccttggt ttcattgaaa aaaaatcaag ctgttttgtc
     4141 agaaacaact attgatgata caggggaatt tgttctttct atcccagaaa atcaactttt
     4201 tgcaggagat gtattagaaa ttgtattaaa cgaccaagca ggagaagcta ctgctaaggg
     4261 tgtgcagaac aagccaagta ccaacgatga aattgggaat cacaatccaa gtgatacgcc
     4321 agttgtttat catgatgccc cgccttttgc acctgctacg aagatcgtag tagaaggtgg
     4381 tctctcattt cttgcccctt ccaaactcga ttttggtcag atcaaagcaa ctggtttgac
     4441 acaagaagca tttggccaaa tggatataca ggaaaaatta tggatttatg atcagcgaga
     4501 aacgaaaaat ccgtggatct taacgataaa attatctcag cctttcacaa atgatgcagg
     4561 attttctatg caggatgtcc tatatttcaa gaaaggagag gagtatcaat tagtgaatga
     4621 agaagaactg gaaattgtca gaggacaaaa ttcaacgaat gtgcaagcag attattcaga
     4681 atatttaaat aacggtcggg cgtttaaact tgtcttaacc aaagaaaatc agatagctgg
     4741 taattactca gcagagataa catggacatt agcagatgcg ccgacagaat aaagatcaag
     4801 gaggatgatt gatgaatcga atatggaata aaagtcatcg aaacaactgc tctttactta
     4861 tgatccttct ccttggattc atcctttggc agacacctgt ccatgcctcc gaattcaatt
     4921 ttgcggtaga ttttgaatta ccggctaatc agattgataa gaacaaaaca tatataaatg
     4981 taaggatgga gcctgggaaa atacaggaaa tcccctacac ggtctttaac cgaacagata
     5041 aatcagtaac agttgagaca ttgatccact ccgctacgac gaattcaaac ggtgtgatcg
     5101 aatatggaga atcaaaaagt aaatttgatg aaacattaag ttatgatatg aaaaacttag
     5161 caaaaacagt gggagaaata actgttcctc ctcaaagtgc ggtcactgag acactttcac
     5221 tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg agggatcacg tttcaagaaa
     5281 aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt caatcagttt tctcaagtga
     5341 aagcaatctt gatttgaaat agcgaaaaag agtcttcccc agatttgaaa ttaaaaaaag
     5401 cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc gaatcttcaa aatccaaatg
     5461 cttcttatat gttcggcttg actacatcgg ttgaaatcta caaaaaagaa acaaaagagc
     5521 cttacctttc aataaaaaaa gaaaaaatag atgtagcacc caatacaaat tttgatttac
     5581 ttgtcccatt agaaggaaaa aaattagctc cgggacatta tcaagcggtt gtgaagggtg
     5641 cttggaaaga aaaacgctgg aatctccata ccgattttga aataactgat gatgcagcgg
     5701 accaattgaa tgaaaaggat attgatttaa aaaatcagcc agaaacattt gactggcgat
     5761 ggctgatact tgttggagtc attcttattg gcttatttgc tggattattt attttaaaag
     5821 aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt accgttctag ctggaagtct
     5881 attattatgt acacaaagtg tttttgctga gaataagagc tcagccacta cagaaggaac
     5941 gatttctgtg accaaaccag atgctgcaaa tcctgaagac gttgttccag ttcctcctgt
     6001 tgatccaaat gatcctgata ctccttttac acctgacgaa ccaaatcagg gaacatccgg
     6061 attactgacg attgaccaag ctccagatct tgattttgga acaatcaagt taggtacagc
     6121 aaaaacagta tatgctgcat tacaaagcgg aacggacagt actggagcac aaacacaagt
     6181 acagaatttc gtacaagtaa cggataaaag cggaaattat gccggttgga aattatcagt
     6241 caaacgaaca gaatttattg gtacaacatc tgatacgaat aaactaactg gttcaaaggt
     6301 gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat agcagtggaa tgccatctac
     6361 cgtttctgga actggcgctg aaggaatcga aatcccagca gatcaggcag tagatctagt
     6421 aactgcagga gcaaatgaag ggattggcac atggatttat tcattaggag agaatgtgga
     6481 acaagggaga caatcagtgg aattatttgt tcctgtatcc aattatgctg tagacaacta
     6541 cgtttcaaca ttcacatggt cgttaacaga tgctcctaca gaagaggaag cgtaaaatca
     6601 tatcaacgat ttcagggagt ggagccgatt caaggcccac tccctgaaat ctgctatgct
     6661 tacgtctcag ctcttattca tcaactcgaa gcagtttttt ggaagtcatg gaaacgttcc
     6721 tttgtctttt caaagatctt tcgaccaacg agtaaggagt caccgttgaa aaattctaac
     6781 acataatgag cagaatcaat tttctgcaat tgcgataagt tgatacagta agattttaaa
     6841 agtaaaaatt ttaggttatc gctgaaagcc ccggaaatac gggcatttcg caggatatga
     6901 gaactcaatt tactaccaat ttactacttt cggattgagc cgcatatatg ctgaaccata
     6961 ctgctaatac ccgccacccc aaatacggca catcataaat tgaatacgac accgtaaacg
     7021 cggcgttcct ctttcccgtc atgggagaac aggacgccgc ttttttatgc ccaaatagag
     7081 aaaggaggcg acgccatgct atttacttcc ggccctgacc gggcgtttga acggctgatg
     7141 caggaaaagc ccggtttcga tcactacgac ggcggcgggg cggacgcgcc ggaggattgc
     7201 ggcacctgcc gattttaccg cccggattgg aaatatcaat tctgccagtt tgcagagtgt
     7261 ccctatcagc cgggcaagct gaccgactat gcagcggtca aattcacagt gaaaggagcg
     7321 aacaacgcta tggcagtttt ccgtgtggaa aagaacaaag gctatacggt gatgtcaaat
     7381 caccacctgc ggaataagaa cctgacctta aaggccaagg gcctgctgtc gcagatgttg
     7441 tccttgccgg aggattggga ctatacccta aaaggactgt cctatatcaa ccgggaaagc
     7501 atcgacgcga tcaggacagc ggtatgggag cttgaaaaag ctggatatat ccggcgtgag
     7561 cagggccgtg acgcaaaagg caaaatggcc gatatggtct ataccatcta cgaacagccg
     7621 gtattggaaa acccggtatt ggaaaatcca acatcggata acccggtgtt ggaaaatccg
     7681 acatcggata atccggcgtc ggaaaatcca acgcaattaa ataaagaagt acaaagaact
     7741 gacttatcaa aaaaagaaaa aataattaca gatggacaaa gtacccattc cattcctatc
     7801 ccatccccga cccctgcccc tatggagggc gaggcagcgg ctacgccgcc ggaacggaaa
     7861 aggacaggaa aggatgaggc tgtacggatt tatcgggaaa tcattttgga gaacatcgag
     7921 tatgcctatc tcattcagga caggagcatt gaccgtgaac agcttgacga aatcgtagac
     7981 ctgatgctgg aaaccgtctg caccgcccga aagacaatcc gtgtcgccgg ggacgactac
     8041 cctgccgagc tggtgaaatc caagtacatg aagctgaccg gcgaacatat ccgctttgtc
     8101 cttgactgtc tgcgggagaa cacaacgaaa gtccgcaata tcaaacaata cctgcgggct
     8161 atgctgttca acgcccccag caccatttcc aactactaca cggcccttgt cgcgcacgac
     8221 atggcacagc ccgattgggg aaagcctaaa tccggcctgc cggactattc ctgcggcgag
     8281 ggcgaaagcc tgtaacgaga aaaggaggac gaccctatga agcagggcgc attgattttt
     8341 gacgagcgaa cagaccgtta cgacatccgc tttgatctgg ccgactatta cggcggcctg
     8401 cattgcgggg agtgtttcga cgtgatgatc ggcggcaaat ggaggcccac ccgcattgaa
     8461 atggccgaaa actggtatct tgtcggcatc cgggccgacg acctttccgg cctgcgggtg
     8521 cggatttgac ctatatctac ctgcggcgtc tggtggttcc gccttagacg ccgcttttga
     8581 ctttctgaca aggagggatg cgattgcagg aagaagttac tcaaaagacc attgccctgt
     8641 ccatgaaaac gggcaagctc acggctcaag tgctgcaagc ggcgctgaaa aagtacctgc
     8701 aacaccgggc caagggcaaa aacacgctgc accacggcca gcagagccta aagcagctta
     8761 aaaagcatgg ggctgatctc tccaacattg agatcaccga ggccaacatt ggagcgttca
     8821 agccatgcgc caagaaatac ggcgtggatt ttaccttacg aaaagataag accacccagc
     8881 cgccccacta cgttgtgatt ttcaaggcca aggatgcaga taaccttgat ctggcgtttc
     8941 gggagtttac cgccaagacg ctttccaagg agcagcggcc ctctatccgc aaggttttgt
     9001 ccgctgtgaa gcaaaaggcg gcggcccaga ccaagcagcg ggccaaggag aaaatcaagg
     9061 aaagggggct ggaactgtga agcctgacgt aaagaaattg ctgatcctga acctcccgta
     9121 tctgattttt gtgtacctgt ttgcgaaatg cggggaggcg taccggctgg ccgctggtgc
     9181 tgacctgtcg gggaagctcc tgcattttgc ggatggtttt gcggcggctt ttgccaaccc
     9241 gctgccaagc ctgcacctgt ttgacctgtg tatcggtatc gccggtgccg tgatcgtgcg
     9301 gctggtggtg tactgcaaga gcaagaacgt gaagaaatac cgcaaaggca tggagtatgg
     9361 cagcgcccgc tgggggacgg ccaaagacat agcgccttac atcgacccca agtttgaaaa
     9421 taatatcctg ctcacccaaa cggaacggct taccatgacc ggcaggccca aagaccccaa
     9481 gacagcgcgg aataaaaacg tgctggtgat cggtggcagc ggttcaggga aaacgagatt
     9541 tttcgtgaaa cccaacctga tgcaatgctt tccgaccacg gactatccga cttcgtttgt
     9601 ggtgacagat ccgaaaggca ctttggttct ggaaaccggc aggatgttcc agcaggcggg
     9661 ctaccgcatt aaaatcctga ataccattaa cttttccaag tccatgaagt acaacccctt
     9721 tgtctacatc cattcggaaa aggacgtgct aaagctggta aataccctga tttccaacac
     9781 caagggcgag ggggaaaagt cagcggagga tttttgggtg aagtcggaac ggttgtttta
     9841 ttcggccctg atcggctata tctggtacga ggccccagcg gaggaaatga acttctcaac
     9901 gctgctggaa atgataaacg ccagtgaagc ccgcgaggac gacccggagt ttcaaagccc
     9961 cgtcgatctc atgtttgagc gtttggagca gaaagacccc gaccattttg cggtgcgcca
    10021 gtacaagaaa ttcctgctgt ccgcaggcaa gacccgttct tccatcctga tttcatgcgg
    10081 tgccagactt gcgccctttg acatccggga ggtacgcgag ctgatggagg acgacgaaat
    10141 ggagctggac accatcgggg acgaaaagac ggtgctgtat ctcattatga gcgatacgga
    10201 cacgaccttt aatttcattc tggctatgct ccaaagccag ctaatcaacc tgctttgtga
    10261 ccgggctgat gatgtgtacg gcgggcggct gcctgtccat gtacgcctga ttttggatga
    10321 atttgccaac attggccaaa tacccaattt cgataaactc attgccacca ttcgaagccg
    10381 tgaaatatcg gcctctatta ttttgcagag ccagtcgcag ttaaaagcga tttacaagga
    10441 cgccgccgag atcatttcgg ataactgcga tagtgtgctt ttccttagtg ggcgcggcaa
    10501 aaatgccaag gagatttccg atgcgctggg gaaagaaacg attgacagtt tcaacaccag
    10561 cgagaaccgg ggaagccaaa cgtcccacgg attgaactat caaaaattag gaaaggagtt
    10621 gatgtcccaa gacgaaattg ccgtgatgga cggcggcaag tgtatcttgc agctacgggg
    10681 tgtgcgcccg tttttatcgg agaaattcga tattaccaag catccccggt acaaatacct
    10741 tgccgacgcc gacaagaaga atacctttga cgtggacaga tttttgacta tcacgcgccg
    10801 gaaacggcag caagtggtaa cgcaggatga agtattcgac ctgtatgaaa tcgacctgtc
    10861 ggacgaggac gccgccgctg aataaacagc ggcttttttc attccaacaa caaaatcaaa
    10921 aaatcattat gagccggaaa ccggcagaaa gaggtaaatt tatggcattt tttaattccg
    10981 cagttactac tttgcagact atcgtgatcg gactgggcgg cgctctgtgc gtatggggcg
    11041 gcatcaatct gttggaggga tacgggcagg acaatccggg ggcaaaatcg cagggtgtca
    11101 agcagcttgt cgctggcggc ggcgtggccc tgatcggtgt cactctggtt cccctgctgt
    11161 ccgggctgct gggctgatcc tggcctttgt ccctgtccaa acccttaact aatcgcctcc
    11221 gcgtcctccc ggccagaccg ggagggcgcg ggaaaggagg tgctacccgt gattagtgaa
    11281 atcattgaag aatggataaa aggcatcctc atggacggta tcacgggcaa cctgtccggg
    11341 ctgttcgata cggtaaatgc caaagtcgga gaaatcgccg ccgacgtggg acgaaccccg
    11401 caggcatgga acagcggtat tttcaatatg ctgcacagcc tgtcggaaac cgtgatcgtc
    11461 cccatagccg gggccatact cgcccttgtc atgtgctatg agcttatcca gatgataacg
    11521 gaaaagaaca atatgcacga ctttgacagc tccatgttct tccgctggat attcaaatcc
    11581 gcgtttgcaa tcctgatcgt aacgaacaca tggaacatcg tcatgggcgt attcgacgcc
    11641 acccaaagcg tcgtcaatca aagcgccggt gtgattatcg ggcagacatc cattgatttt
    11701 aacaccctga taccagacct tgaaagccaa ttggaggcta tgagcgtggg cgctcttttg
    11761 gggctgtggt tccaaaccct tgttgtgggc ctcaccatga atatcctttc catctgcatt
    11821 ttccttgtga cctatggccg catggtggaa atctatgttg tcacgtcgct ggggcctatc
    11881 ccccttgcca caatgggcaa cgcggaatgg cgcggcatgg gccaagggta tctgaaatcc
    11941 ctgctggcgc tgggcttcca agccttttta atcatggttg tggtgggtat ctacgcggtg
    12001 ctgatacaga atatcggttc cgcttccgac atatccggcg caatctgggg ctgtatgggc
    12061 tatacggtgc tgctgtgctt ctgcctgttc aagaccggca gtatatcaaa ggccgtattc
    12121 ggggcgcatt gatcccaaaa tccaagcata cacaggagga acttgtttat ttcaacgtac
    12181 aacatcattt acgctgaccc gccgtggcgg tatgactgca aaaacgtaca gggcgcagcg
    12241 gaaaatcact attccaccat gagcattgac gagctgtgcg ccctgccggt ggaacggctg
    12301 gcgtccaaag actgcctgct gtttttgtgg gccacattcc ccatgctgcc ggaagctctg
    12361 cggttaatca aagcgtgggg cttttcattc aagaccgtgg cttttgtatg gctgaaacag
    12421 aaccgtaaaa gccttacatg gttttacggg ctgggccgct ggacaagggg aaacgcggaa
    12481 atctgcctgc tggcaacaca cggcaagccg aagcgtcgtt ctgcctctgt ccatcagttt
    12541 attatcagcc cgattgaaca acacagcaaa aagccggatg tgacccgtga aaaaatcgta
    12601 gagctggcgg gcgatttgcc ccgcgtggag ctgtttgcca gacagaaaac ccccggctgg
    12661 gacgtgtggg ggaacgaggt ggaatgtgac cttatcctga cgtgaaagga ggtgccaaat
    12721 ggcttatgta accgtcccca aggacttaac ccatgtgaaa agcaaggttg tgtttgggct
    12781 taccaaacgg caactgatct gtttttccgg ggcgctggtg atcggcgtcc ccttatattt
    12841 tctcacccgt gactatctta ccaacagcgc ggcggccctg ctcatggtat ttgccatgct
    12901 gccgggcttt ttgcttgcca tgtacgagcg tcacggccag ccccttgaag tggtgattat
    12961 gcagatcatc cagtgctgct tcatccagcc aaaggagcgt ccctatcaga ccgacaacac
    13021 ctacaccgcc cttgtgcggc aataccagat ggaacaggag gtaaaggcca ttgtccaaaa
    13081 aggaaatccc aagagaaacc gaaaaaacaa agctgacccg cgcccagcga aaagaaattg
    13141 acgcggtaat ccgtaaatat aagggcgacg gcaggcccca cacggcgcag cagagcatcc
    13201 cctacgaggt catgtacccg gacggcgtgt gccgggtatc gcccggcgtc ttttccaagt
    13261 gcattgagtt tgcggacatc agctatcagc ttgcccagcc ggacacgcag acggctatct
    13321 ttgaaaaact gtgcgacctg tataactatg tggacgcctc cattcatatc cagttttctt
    13381 tcctcaatcg gaaagttgac ccggtgcagt acgccaagag ctttgagatc gcgccgcagg
    13441 gggacgactt cgacgacatc cgggccgagt acaccggcat tctgcaaaag cagcttgcaa
    13501 acggcaacaa cggcatggta aagaccaaat acctgacatt caccatcgag gccgaaagcg
    13561 tgaaagcggc gcgagccaga ttgaagcgca tcggctttga cctgttaggc tatttcaaga
    13621 gcatgggggc cgtagcccat gtgatggacg gctgggaacg gctgaacctg ctgcatggcg
    13681 tgtaccaccc ggacggcgag atattcaatt ttgactggaa gtggctggcc ccgtccggcc
    13741 tctccaccaa ggattttatt gccccgtcct ccctgtgctt cggcaatgca aagacattcg
    13801 gcatgggcgg caaatacggc gcggtaagtt tcctgcaaat cctgtccccg gagctgtcgg
    13861 acgatatgct ggccgacttc ctcaatacgg aaagcggtgt tttagtcaat ctccatgtgc
    13921 aggccattga acagaccaag gcgatcaaga ccatcaagcg caaaattacc gaccttgacg
    13981 ctatgaagat tgcggagcag aaaaaggccg tccgcagcgg ttacgatatg gacattcttc
    14041 ccagcgacct tgccacctac ggcgaggacg ccaagaagct gctgacaaag ttgcagaccc
    14101 gcaatgaacg gctgttccag ttgacgttcc ttgtcctcaa cgtggccgac accaagcaaa
    14161 agctgaataa cgacgtgttc caagccgcag gcgtggccca aaagcataac tgtccccttg
    14221 tccggctgga ctaccagcag gaacagggcc ttgcaagcag cctgccgctg ggcgtcaatc
    14281 agatcaagat acagcgcagc cttacgactt ccagcgtggc cgtgttcgtc ccctttgtga
    14341 cgcaggagct ttttcagggc ggcgcggcca tgtattacgg catcaacgcc aagtcccgca
    14401 acatgattat gctggacagg aagcaggccc gttgccccaa tgccttgaag ctcggcaccc
    14461 ccggcagcgg caaatccatg agctgtaaat ccgagatcgt cagtgtgttc ttaacgacgc
    14521 ctgacgatat ttttatttca gacccggagg ccgaatatta cccccttgtc aaacggctcc
    14581 acggacaggt aatccggctg tcgcctacca gcaaggactt tgtgaacccg ctggacatca
    14641 acctgaatta ttcggaggac gataacccgc tggccctgaa atccgacttt gtgctgtcgt
    14701 tctgtgagct tgtcatgggc ggcaagaacg ggctggaagc cattgaaaag accgtgatag
    14761 accgggctgt gcgcgtgatc taccggccct atctggcaga cccccgcccg gagaatatgc
    14821 cgatcctgtc cgacctccac aaagcgttgt tagatcagca tgtcccggag gctgaccggg
    14881 tggcgcaggc ccttgacctg tatgtgtccg gctccctgaa cgtatttaac caccgtacca
    14941 acgtggacat cggcaaccgg cttgtgtcct ttgacattaa ggagctgggc aagcagctca
    15001 aaaagctggg gatgctcatt gtccaagacc agatatgggg gcgcgtcacg gccaaccgaa
    15061 gccaaggaaa ggccacatgg tattttgccg acgagttcca tttgcttttg aaagaggaac
    15121 agacggcggc ctattctgcg gagatttgga agcggtttag gaaatggggc ggcattccaa
    15181 caggtgccac ccagaacgtc aaagacctgc tttcctcccc ggaaattgaa aatatccttg
    15241 aaaacagcga cttcattacg ctgctcaatc aggcgtccgg ggatagaaaa atccttgcgg
    15301 aacgcttgaa tttgtccacc gaacagcaga agtacatcga caattccgaa cccggcgagg
    15361 gccttttgat ctttgaaaac gtggtgctgc cctttaccaa ccccatcccg cacaacaccc
    15421 agctttacaa gattatgacg acccgcttaa acgaggtggc gggagtatga cccggcgacg
    15481 ccacaaagga ggtgaaccac catgaaagag ctgaaagcca aggcgaaagt gacgcaggcc
    15541 atgacccgcg acgggctgac gctggaaaat caggccaccg gcgaggtgga gaacatttcc
    15601 agccgggagg ccgaacagga tttgtccgcg ccggggccgg gcggcaccgc cgataagctg
    15661 ctggaacggg ccgacgccat ccatgacggc cataaggcca agaaagccgc gaagcaggcc
    15721 ggggctgtgg ccgccgaggg ggacgctgcc ctgcaccggc catccgcccg gctccaactg
    15781 tcggaggaag aacgggccga cccggattta cagccctata tccgaaaatc ggacaaacgg
    15841 gccgataagc tggataaggc ccgcgccgcc atccccaaga agcgcgtccc ggtcaaggaa
    15901 accgtctttg acgaggcaag cggcaaagca aagaccgtcc tgcactttga caaacaggac
    15961 aaggggcctc cctccctgaa acccaatccg gcaggccgtc ctgtttccga ggtttttcta
    16021 ttcggccacg ggaaaatcca tgaagtcgag catgaaaacg tgggtgtgga gggcggccat
    16081 aagggggagg aacttgccga gcggcaggcc ggaaaagcca tccgcagcac catccggcac
    16141 cacaggctga aaccctactg ggacgcggaa aaggccgagc gcaggctatt gtctgccaac
    16201 gcggagtatt tctaccagaa atctttgcgg gataacccgg agatcgcaca ggcggccagc
    16261 aaccccattt cccggttctg gcagaaacag cagatcaagc gccagtacgc caaggccgcc
    16321 cggacagcgg gccagaccgc aggcgcggcg gcacagggcg cggccaagac agccggggcc
    16381 tccacgaaag cggccaaggc cgcagccaaa gaaagtgaac gggcggcctc ctttgtggcc
    16441 cgccactgga aaggggcgct ggccgtcctt gtgttggggc ttttgatgct gcttatcatc
    16501 ggcggcttac agtcctgcac cgccatgttc ggcagcgccg gaagcggcat cacggcgtcc
    16561 tcttactttt ccgaggatag cgatatgttg gccgccgagg acgcctacgc ggagctggaa
    16621 gccgagctgc aagactatct ggacaactac gaggccaccc atgactatga cgaataccat
    16681 tttgaccttg acgagatcag ccatgacccc tatgtgctga tttccattct gtcggcgctc
    16741 catgacggga tatttaccat cggggaggta cagggtgatt tgtccatgct ctttgacaag
    16801 caatatatcc tgacagaaaa cgtcgtggta gaaacccgtt accgcaccga aaccgataca
    16861 tggacggacg cggagggcaa cacccataca gacacctacc aagtgccgta tgactattac
    16921 atctgctacg tcacgctgga aaacttcgac ctgtcccatg tgccagtcta tatcatggac
    16981 gaggaaacgc taagtttcta ttccctgtat atgtccacgc taggaaaccg gcctgatctg
    17041 ttttccggca gccagtaccc caacgcctcc accctgacgc cgccgaccta ctacgacgta
    17101 cccccggagg ctttggagga tgccaccttt gccgccatgc tcaaagaggc ggaaaaatat
    17161 atcggttatc cctatgtgtg gggcggcagc tctcccagca ccagctttga ttgcagcggc
    17221 ttcatttcgt gggtaatcaa tcatagtgga tggaacgtgg gacggctggg ggcgcagggc
    17281 ctatataaca tctgcacccc tgtttccagt tcgcaggcca agcccggcga ccttgtattt
    17341 ttcaagggaa cctacgacac ccccggcgtg tcccacgtcg gcctgtatgt gggcaacaat
    17401 gtgatgctgc attgcgggaa cccgatttcc tacacgaacc ttacttcaaa ctactggcaa
    17461 tcccattttt acagttacgg gcgtttacct taacgccaga aaggagcttt caattatggc
    17521 aaccaaactt gaccgcatcg aaaaggacat ccagaaaacc aagtctaaga tcgcggagtt
    17581 tcagaaacag cttcgggagc tggaaacgca gaaaaccgaa caggagaatt tgcagatcat
    17641 ccagcttgtg cgcggcatga acatgaagcc ggaggaattt gccgcgttct tacgcagcgg
    17701 cgcaatgcag gccgcccctg ccgcaacccc ttaccatgaa caggaggaca atgcccatga
    17761 agaataagac cagacggatt tcccgtattc tccccgcact tctggccgtg atgctctgcg
    17821 tcacggcctt tcccgttacc gcccttgcag gcggcaatga cccggcaccg gccccgctgc
    17881 cggaggctac aaccgaaccc gctacgggcg gtttggagcc tgaaacagac gaaaccggca
    17941 ccgccacgga cgggacagaa accgaggccg aaaaagatac cggccctctg accggcaagg
    18001 acatcaccga cctgttttcc gcgctgttcg gttccaaggt gagcattgcc gccacggacg
    18061 acggcatcca gatcaccacc ggcaccgaca aaaaagaacc tgaacagacc ggcaccgtca
    18121 ccaccaacgg cggcaggctg aatgtccgca ccggcgcggg gcttgacaag accgcgttca
    18181 cccagcttcc caatgggacg acggttgagg tagtcggcac agacggcgac tggattaaaa
    18241 ttctcttgcc ggagcgcatc ggctatgtcc attccgacta tatgaccgtc agcgaaaagg
    18301 aagtagcggc taccggcgag ggcggttttt ccctctccat tgacccggag gaaattgctt
    18361 ctctgctgga actgttcaat ggcggcgggg ccagcgccgc cctgtccccg gacgggaacc
    18421 tgtcactgat tgacgacatc ggcagcgccg cgaaaaatgg caagcagttt atcaccgtgg
    18481 aaaccaaaaa cggcaatgtg ttttatctga ttattgaccg ggacgacgag ggaaaagaaa
    18541 ctgtccattt cttaaatcag gtggatgaag ccgatctgct gacgctgatg gaggacgggg
    18601 aaaccgtcac ccccgctgct gtctgctcct gtaccacgaa atgcaaggcc ggggctgtga
    18661 acacgaattg cccggtatgt aaaaccaacc tgaccgagtg cagcggcccg gagccgcagg
    18721 aaccccagcc ggaggaaccc gaagccccgg aggaagaacc caagggcggc gcgggcggcc
    18781 tgatcgtgtt cctgctggtg gcgctggcgg gcggcggtgc ggccctttac tatttcaagt
    18841 tcaaaaagcc caaggctgaa accaccggca gcgacgattt gggcgagtat gattttgggg
    18901 aggacgagga tttggaggac gaggaacccg aaaccgagtt tgacccggaa gctgattttg
    18961 tgagtgaaca ggaggacgag gaatgacata taaacttgta atcgcggaaa aacccagcgt
    19021 ggcccgcagc attgcgggcg tgatcggggc cacggagaaa cacgacggct atttggaggg
    19081 gaacggctat ctggtgagct ggtgcatcgg acatctggtg tcctttgccg acgccggacg
    19141 ctatgacgag cgtttcaaaa aatggcgcta tgaggatttg cccattctcc cggaaacatg
    19201 gcagtacatc gtccccgacg acaagaaaca gcagtttgac gttctctgtt cgctgatgca
    19261 gcgccccgac gtgaccggcc ttgtgtgcgc caccgacgcc gggcgcgagg gggaattgat
    19321 tttccgtttc gtctaccaga tggcgggatg ccaaaagccc tttgagcgcc tttggatttc
    19381 ctctatggag gacagtgcta ttaaggacgg ctttgcccac ctgaaaccgg gcggcgacta
    19441 tgatccgctg tatcaatcgg ccctgtgccg ggccaaggcc gactggctga tcggcattaa
    19501 cgccacccgg cttttttccg tcctgtacca caagaacctg actgtgggcc gcgtccagac
    19561 gccgacctta aaaatgctgg tggacaggga cgccaagatt accgatttca agaaagagaa
    19621 atatcacatc gtccatatca cggcgggcgg cgcggacgcg gtaagcagcc gcttttccga
    19681 cgctgccgag gcaaacaccg tcaaggcggc ttgtgcaaaa gcgcaggccg tttgcgtttc
    19741 cgtgacgcgg gagaaaaaga cggaacagcc gcccaagctc tatgacctta ccaccttgca
    19801 gcgggaggct aaccggcttt tcggctatac cgccaagcag acccttgact atgcccaggc
    19861 actgtatcta aagaagctgc tgacctatcc gagaacggat agtcaatatc ttaccgacga
    19921 tatgctgccc gccgtggaaa gccttgtgtc cggcctgtgg gagctggtgc cgtttgccaa
    19981 gggcctgaac ctttccccgc agtttgaccg catcctgaac agcaaaaagg tgtccgacca
    20041 ccacgccatt atccccaccg ctgaatttgt gaagcagggc tttggcactc tggccgagag
    20101 tgaaaaaaag ctgctgtccc tgatctgctg caagctctta tgtgccgtgg ccgggccgca
    20161 tatctatgag gccgtcaccg ccacatttac ctgtgccggg caggagttta ccgccaaggg
    20221 caagaccgtc ctttcccccg gctggaaaga gattgaacgc cggttccgtt cttccttaaa
    20281 gaccgacgcc gacgaggacg ccgaggcggt gcgggagctg ccggagcttt gcgagggcca
    20341 gacctttgca gacgtggccg cctctgtcac cgagcatttc accacgccgc ccaagcccta
    20401 caccgaagat acgcttttgt ccgctatgga acgggccggg gccgaggata tgccggagga
    20461 tgccgagcgc aagggattag gcacaccggc cacccgcgcc gccattctgg aaaagctggt
    20521 gcagatgggc tttgtccaga gaaaaggcaa gcagctcatt cccaccaagg acggcatcaa
    20581 tctggctgtg gtgctgccgg aggcgcttac ttctccccag cttactgccg aatgggaaaa
    20641 ccggctgacc gagatcgcca aggggcaggc cgatcctgac gaatttatgg acggcatcga
    20701 gacgcaggcg cgggagctgg tgcagaccta ttcctgtatc agtgaggaaa agcagaaatt
    20761 gtttcaggcc gagcgtgttc ctatcgggac gtgtccccgc tgtggtgagg ctgtctatga
    20821 gggcaagaaa aactactact gcggaaaccg ggcctgccag ttcgtcatgt ggaagaatga
    20881 ccgatttttt gaggaacgca agaaagcgtt caccccgaag atcgccgctg cgctgctcaa
    20941 aaccggcaaa gtcaaggtaa agggcctcta ttccgtaaag accggcaaaa cctacgacgg
    21001 gacggtgctt ttggctgata ccgggggcaa gtacgtcaac taccgcattg aacacaggag
    21061 ctaaaggaca gtaacacagc aagcataggg agtgtggcta cactcccggt aaaatccccc
    21121 ggccgcgtcc ctgccgggcc ggggattttt gcttgttggg gaagtcccca accccctatt
    21181 ttttgaaaca gaacagagaa aggagtgacc gcgtgaacga agtattttct atccaagtcc
    21241 ataaccggca gtttttggaa cagggcaaac aaggcgtctg gctggatttg cccaccacaa
    21301 ccgaaacgct gcaagccgcc ctgcgggatg ttggcattac tgccgacaat ccgcaggatt
    21361 tttttatcaa cgggtattcc tgcccggagg acagacacct tgccattccc tatgacatgg
    21421 tgctggccgc cgacgtggac gagctgaatt tccttgccgc ccggctgggc cagcttgacg
    21481 ccgccgagat cgcagagctg aacgccgccc tgcaaaatcc ccgtggcggc tttgagaata
    21541 tcggccagat catcgactac accgaaaatg tggattacta tgtgcatctt cccgacgtta
    21601 ggggggatgg acagttgggc gactactacc tgaaccgttc cggcatggtg gatatgccgg
    21661 aggaatggaa agccgggatt gatacggcgc agtttggacg ccacattgcc cagcaggaac
    21721 aaggcgtttt tacccagtac ggctatctgg tgagaagcgg cgacgaatgg cagcgcgtcc
    21781 atgagggcca gccggtgccg gaggaatacc gggtgatgca ctacccccag ccggaagctg
    21841 accgggagga accgcaggcc cagcagccgg aggcatccga aagccccgac aaagcgccgc
    21901 ccccggttat cccgatcatg ctggacggca tgaacagcaa agaacgcatg aaagtgatta
    21961 ctgaccgtct ggaaaccggc gtacaggagc tttttgaaag tagccgctat atggcttact
    22021 tatccgctat gtccaagatg cacgattaca gtctgaacaa tacgctgctg atcgccatgc
    22081 agggcggttc gattgtggcg ggctataaca ggtggcagga tgaatttgac cgccatgtga
    22141 agcggggcga gaaaggcatt aagatactgg ccccggcccc gttcaaggtt aagcagcaga
    22201 tggagaaaat cgacccggcc acccagcagc ccgtgatcgg cgcggacggc aagcccgtga
    22261 ccgaagaaca ggaagtgacg gttccggcct ttaaggtggt gacagtcttt gacatcagcc
    22321 agaccgaggg caaggatttt cccatgccct atatcagtga acttaccggc gacgtgcagc
    22381 agtatcagga tttctttgcc gcgctggaac ggacatcacc ctatcccata agcattgagc
    22441 cgatttcaga cggggccaag ggccgctgct tctacgcaaa acagcgcatt gccatcaacg
    22501 agggcatgag cgaattgcag accctaaaga ccgccatcca tgagattgcc catgccaagc
    22561 tccacgccat tgaccgggag gcccccgccg aggaacaggc ggcccggcca gaccgcagca
    22621 cccgcgaggt acaggccgaa agcgtggcct ataccgtctg ccagtattac gggctggaca
    22681 cgtcggacta ttcttttggc tatgtggccg attggagcag cggcagggag ttatccgagc
    22741 tgaaagcctc ccttgaaacc atccgggcca cggccagcga attgataacc gagatcgacg
    22801 gccattttgc ccagcttcag caggagcgcg aggcccggca ggaacaggag gccgtcacgg
    22861 ttgacgttac cccggaggca gcggccccgg cgcaggccga ggaagaacag gccccggccc
    22921 ttgatccggc agccgagccg cttgtgactg tgctatggag cgaaagcccc cagctaaagg
    22981 acggccagca aatgccgttg catgaggccg acgcgctgtt tgcccgtctg gatgcagcat
    23041 atccggcaga ggcaggctat gacaaaaccg cgttccgcat tgactgtatc tttcagggcg
    23101 agccggacag ctacgagggg cggcaggatt ttggagatag ggacggttcc ctgatcgaac
    23161 atatccagag ctaccaccaa ttttacgcgc aggacgaaaa ctggaaaaag catgtaatcg
    23221 ctacaaccgg catagagggc tgggagcagg acaaggccca tcgggatatg ctgttaggcg
    23281 agtttgtccc ctatatgaag ctgcattgca gccttgccca aatggaacag acggcgcagg
    23341 gccgcttaca gtccggggaa accctgacgc cggaggaaac ggcctatttg ggggcgcttc
    23401 tctcttatgt gcaggagtgc cgccccatgc tcaatcaggg ccagtatcag ttgccggagc
    23461 cgccgaaact ggctgacttc gaccagagct tgcaggacta caaagcacag gtacaggccg
    23521 agatcgcgca ggaggccgcc gcagccggta tgaccgtgga ggaatacgcg gcggctggct
    23581 atgaagcacc ccaacaggac accttttcca tctaccagct tcgaggcggg gaggaaaccc
    23641 gcgactatcg gtttgagcca tacgagcgtt tacaggccgc agggctggcc gtggacagtg
    23701 ccaactatga ccttgtttat acggcaccgc tggataaaag cacgtctttg gaggatattt
    23761 accgcacatt caacatcgac cacccggcag attttagggg acacagcctt tccgtttccg
    23821 acgtggtggt gctgcaccag aatggacagg acaccgccca ctatgtagac agcttcggct
    23881 atatggaagt gccggagttt ttgcgggaaa atcccctgcg taccgccgaa ctgtctacgg
    23941 agcagaacga aaacatgatt gacggcatta tcaacaatac gccgcccgaa ccgcagccgg
    24001 aggaacccga acagctatct tttgaggatg tggcagttgc ttccggccag acacaggagg
    24061 aacccccgga ggctgcgccc ccgcaaacgg ctgtccgcta ttatcccatc aatgagggag
    24121 ccgcccgccg cgccaaggaa gccatgagct tttctgacta ccggcccggc agcgccacgg
    24181 cggaataccg gcactatgtc gatcaggccg tggaaattgc ggagcgccag aaaaagcgtg
    24241 tggagccaga gtatcacgaa aagatagaca gcctccttga cacctacgcc cgcaagctgg
    24301 ccgagaacat gaataagggc tatgagatta ccgcccgcgt cccctctgtc ctgattgccg
    24361 ggccgtccaa tttcccggta cgggcgaaag aaaagcagaa cgccgccagt gaccgcaaca
    24421 tggaggaatt tcagtacatt cagggcttgc ttgacaaaat ccgcagcacc ggcatgggtg
    24481 gtatcagcgc cgacgatccg caggccgttt ccaagctgga aaagaaactg gaaaagctgg
    24541 aagcgtcgca ggagttgatg aaggccgtca atgcctacta ccgcaagcat gggacgctgg
    24601 acggatgccc ccatctgacc gaaagaggca ttgaaaacct gaaagcggat atggcgtcgg
    24661 gctggcacta tgagaaaaaa ccgttccagt cctggcagct ctccaacaat aacgccgaga
    24721 tacgccggtt aaagggccgc attgaggaat tgacccgcca aaaggaggcg gcctatgtgg
    24781 gatgggagtt tgacggcggc actgtggaga tcaaccgggt ggccaaccgc ctgcaaatct
    24841 tctttgaggg gaagccggat gctgctgtac gcgacgagct gaaaagcaac ggtttccgct
    24901 ggtcgcccaa ggccggggca tggcagcggc agttaaacga caccacaatc cgcgtcgctg
    24961 accgtatcaa gtgcatccag cccctttccg gcgaaaagcc cagcgcctta cagcttgcgg
    25021 cccgcaggga gaaagaaaag ccctctatcc gggcgcagct caacgcggcc aagaccgaac
    25081 aggccaagca gccaccggcg cgggaaaaat caaagggatt ggaggtagag taaatgcaga
    25141 agtttgaaaa tgtggacgtt ctggccgcgc tggaacagat catgcggcag aacaccgcgt
    25201 tttatcaaag tgactttggc attgacaaag acattatcag gcgggatgcc gccagcgatc
    25261 aggccgcaga caagaccctg ctctggatgt cccggccctc cgggacgtac tgtttccggg
    25321 aacgtgatgt tttcctgaaa gacacccgac agtacaacac atggaaattt tacggggagc
    25381 agacccgtga caagatactg gcctacgcgg tggagctgac cggcacacag ggcggcacta
    25441 tccggggcaa tctctatgag ctggactatc cgcagcattt ccggcacgtc attgaaaccg
    25501 cccagccggt gagtgtgaac cggctctttt atgagcatgg cacccgtgac atcccggaga
    25561 gccagcgttt tgacggaagc cctgaccggg tattgggaaa ctttttgtac tatgaggccc
    25621 agccccatga cccggctgtg ctgcaggagg cgctacggac ggagcagcac agccgggggc
    25681 gggccgtccc cggcgatttc aaagtccacg tcaccgcttt gcatgacagc cggattgaaa
    25741 ccgaggcccg gcgtattgtg gacagcctga aagatctgtc cgcgcctaac agccccaata
    25801 aaacgcattt catggtggag atctccccgc actttgcggc gattgcttcc accaaggata
    25861 atgaccggct ctttgccatg ctgccctata aatccctgtg ttttaccggg atgaaaggca
    25921 ggcatggcgt gtatgccgtg attaaccggg acgagcgccg ggatgtgagt attcgcaggc
    25981 cccggccctc tatccgcaat cagctatccg acagcaaaca ggccgccagc ccaaaaaagg
    26041 cggcggccag aaccaaaaag aatgaattgg aggtataagg aatgatgcac tttaccgtgg
    26101 aggaagaaaa cctgatctgt atgtaccaca atgcagaccg gcgcagaacg ataaacaggc
    26161 tcaccgccgt catgccggtt atggatgggg atatgcgggc gcaggcccag cagacccttt
    26221 ccaagctgga acgcatgacg gatgccgact atgacggcca gaagttccac ttcacagacg
    26281 aataacgaac agcagacgcc gggcgcggcg gccatgtggt cgcttgcgtc cggcgtcttt
    26341 tctttttgcc tattcctacg catttagaac ccggtattgt cggggatagg atgaagtatt
    26401 cgcttgcccc tgcaaacatg cgggagaagc cttgcagcgg gcggttttca atcccttaaa
    26461 tgcgtaggtt gcagctataa ttagacccct gaaacagata tagccgcgca gcgggtgtat
    26521 ctgtttcagg ggtgtgcagg tgatagccgc aaagcggcgt aagagggtgc agccctcttg
    26581 ccgtctatct ctgttccagc gggaagctcg gcaatgcttc cagcagtttc agggaaatgc
    26641 gctgccgtat atcttcgtca atgactttct tctttttgcc gtttgcctgt ctgatctcca
    26701 ccgtggaaag ctcgttgatg tagtcagcat aatgggccaa cactttttcc gtggcccacc
    26761 tctcaccggc aacggcggct ttgatcgttt catagtccag cagcttttct tgtccgtcca
    26821 tcctgatacc cccgtcagac tgattttttc tatgatacca tattcggaag catggaacca
    26881 gagaaaacgg cgcttttctg ctggtaaatt ctacgcattt agaacagcac ttcggcgggg
    26941 ataggataaa acacccacat accccgctga ccatgccgga aaagccttga aatataaggg
    27001 cttttacagc tctaagtgcg taggtttcca gtatcttttc cgctggcgct cggcctcttt
    27061 gcggcggcgc atccgtttgg agcaggccgc gcagtatttg gcccggttag acttcggcgt
    27121 gaacgtggcc ccgcactcac agcagcgttt cacttcgtcc cggtctttgg tgatctcggc
    27181 atacagggcc gcatccagcg ggaggacagc ggccttaaac cagcggcaca gaagcgaata
    27241 ggaaatgctc tgtacgcaaa tacattcttc gccgtcgtcc agcaacaggc agttgccgtc
    27301 acaatagttg cagcactcat gcgtcagctt ccgggccttg cgatattgag ggtaattcat
    27361 tcgtggaata ctcatagctc ttgcgtccat tcctttcccg gcgtcatacg caaaatgccg
    27421 tccacattct gcttgatgat gccgtattcc tgcatctgct ttttcgcttc gccgtacttc
    27481 tcatacagcc gttccttttc ctcggccaat cgcttgtact cggctttcag ggcgtacaga
    27541 tcggggagct ttttcacacc ctgtcccttt agggcctttg ccgcagcttc atacaaaatc
    27601 aacgtgcttt catgctcacg ccggaatttt gccttatccg ctgcattgcg gtattccata
    27661 gccacgggcc agagctgctt gtaggtagaa atattcttta tcagcaccgc catatccgca
    27721 agccggtgtt ccatagcttt gagggtagcg gcggcctcgt cgctggcggc ggtgatctcg
    27781 gccactttgc tttccaaatc cgggtaggtg tctatcccat gttcagtcag gaaattcaaa
    27841 gtctttgcgg cctgcttcaa attgtggagc ttcgcccacc tctcatagcc tgccgactgc
    27901 tgggccttaa tgttgttttc cagatcaatc cgcaaagaaa tccccttgcg ctctttgggc
    27961 gctctggtga taacgcggcc tttgatacgc tctttgatgg cttcctcggt gtaggcttcc
    28021 cccaatgtct tgcagcgggt aaagcgttcc tgtccgggag cgcggaaaga aataaacttt
    28081 cccggcttga tctcatagcc ctgcgcctgc atgagccgca ggaaatcatc aaaatctttt
    28141 gcctgcggga tagcggcgtc aatggctgct ttgagcttcg ctttccagct cgtccctttc
    28201 cgctgggcgt cccactcggc atagctcttg cccttactct ggccgggctt caccacggac
    28261 aatccgtttt ccttgcacag cctgtcgctg gtgcgccgga tataggcata gctctgtttg
    28321 ttggaaatgt acttgcgctg atttaccata tccacggcac agaaaataat gtgattgtgg
    28381 acgtggccct tatcaatatg ggtagtcagc acatagggat atttcccttg cagcacttcc
    28441 tcggcaagct gcttcccgat ctcatgggcc tgttccggcg tggcttcgcc gggggcaaag
    28501 gattgtatca ggtggtggcc cagattattg cccttttggt atgcctgcgc cagcagcatt
    28561 tcaaattcaa tgtccgctgt ttcataagag caggcaaagg aggccaccag cagcttgccg
    28621 tcagttttgt ccgggttctc gatgtagtca agggcttttt tcagcgttga cttgatagga
    28681 tgtatctttg taactgccat atctgcgcca gcgccccctt tatatcctcg atgtcctgtg
    28741 cgtacaccgg ccccataccg ttcagcctct ttgcaatctg attgacgttg acgccgatct
    28801 tctgtatctc ggcggtctgc gcccggatgt cgctggtgtc catgttgacg atatagccgt
    28861 caatgagcat cttccgggcg taggctgaaa agttcttcgt atgcagcagc gccattttct
    28921 gttcaatcat agcccgttcc tccggcgtca cagggacgcg cagcacgatt tttcgctttc
    28981 tgtttgccat gcttcaccgt cctttccatt caggggtttg gggatttccc caacaagcaa
    29041 aacggacgcg gcccggcagg gacggggcag gccgtttttc cgggagtggc tacactccct
    29101 atgcttgcta cgttactgtt tctccactaa caagacgttt ccaaagcccc aaactacccg
    29161 caaaatagcc ctctggaaag aaaaaagtgc aaaaaaatag aagctacgct gtgcagctcc
    29221 cgctaaaagt aaaacagagg atgccagtta cacatcctcc gtttcattct tcttcggctt
    29281 ttcaattagt atggcgctgt caatcgcgcc ctcgatgatc ggcaggtatt cttcggggca
    29341 gagtttcagc ttgtggctga ccctttgccg cagttcgctt tcctcccgca tgatctccgg
    29401 gttaaagtag cgttccacag gaagcccgca ggctttaata agctgtatga caaccggcag
    29461 actgggaatg gccccgtcgt tttcgatgtt ggcaagatag cgccagtcaa tatttatttt
    29521 ctctgccatt gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca catccgcgcc
    29581 gaaagtttca aagccgggac aatcttcaat attcgccata tcgcacctcc cacttacatt
    29641 gtatatttca tactctgccc gtggaatgtt ggtatatgca cctttcttaa agtttatatt
    29701 tcacattttg tttcttttat tcttcgagca aaagaattat aatgtaccct gtggaggtgt
    29761 actatggact acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg
    29821 ataaactact attgtgctgc gggacgcatc cccggcgctg taaagatggc gacgatctgg
    29881 ctcattccca aaactgcgga aaagccgatt gacaggcgta caaagaaagg aaaggcggtt
    29941 actgaatagt ataatgaaac cggcgcattt tggatatggt tttgaagaga gagaaagcat
    30001 ggtattttgt cacggaaaaa ctataagatg atagatggca ggctgctcca gaccgataaa
    30061 aagtattctc atctgaaaca gaaagaaaag atttacacat ggatgtatga agaagcgaaa
    30121 ctttatcatg acagaaatgg aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc
    30181 agccgtatat ggtagaattg aaaaggccgg catctggatt ctttatggtg aagtctccag
    30241 acattggata actgctggtc taatgcgcct ttgagtatgg ggtgattgat tttaataccg
    30301 gggaggtacg gctttttgag gaaaatattc tgggatttac atttggcgat tattacattt
    30361 cctgtaactg caaggacacg gatgatgaag atatgaggaa tatagacttg ctgattttct
    30421 actgtcttga acggtattag gactgaatct gctgcgcgga gtgcggcaag agcgaccgga
    30481 gggagtacac acaggccctg cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa
    30541 agaggccgcg cattttgctg aggcttgggg tgtccccaac aagcaaaacg agaacgccgc
    30601 aggcgggcaa gtggtggatc agaatatccg taaaggatat tctgatccat tgcttgccag
    30661 tattatcggg atgttttgac aaccgctgta atcgaaacat cccaaacatt aagaaggtgt
    30721 taattattat tatcttgatt taattcgttt aaaccaataa tatagataca tacaccatga
    30781 aaagaagcga gtatcccaat gacggatgat taaaatccga aaagaaatcg ctttgaaact
    30841 acagggaaac tacagactgt tatgttatct tcttaaaatg gagggatttt tatgtcgata
    30901 cgaattctac ttgtcgagga tgatgatcat atctgtaata cagtaagggc gtttctggct
    30961 gaggcaggat atcaggtgga tgcctgcaca gatggaaatg aggcatacac caagttttac
    31021 gaaaacactt atcaactggt tattcttgat attatgctgc ccggtatgaa cgggcatgaa
    31081 cttttgcgtg aatttcgtgc gaaaaatgat actcccattc tgatgatgac agccctgtcg
    31141 gatgacgaaa accaaatccg ggcgtttgat ctagaggcag acgactatgt aacaaagcca
    31201 ttcaagatgc agattttact aaagcgggtg gaagccctgc tgcggcgcag cggtgcgctg
    31261 gcaaaggaaa tccgtgtcgg caggctgaca cttctgccgg aggatttcac ggtactttgt
    31321 gacggtacag agctgcccct gacacgaaaa gaatttgaaa tccttttgct gctggtgcag
    31381 aacaaaggca gaaccttaac gcatgaaatc attttgtcac gtatatgggg atatgacttt
    31441 gagagtgatg gcagcacagt ccacactcat atcaaaaatt tgcgggccaa gctgccggaa
    31501 aatatcatca aaaccattcg cggtgtagga taccgattgg aggaatcata ataatggaaa
    31561 gaaaagggat tttcattaag gttttttcct atacgatcat tgtcctgcta ctgcttgtcg
    31621 gtgtaacagc aacactgttt gcacagcaat ttgtgtctta tttcagagtg atggaattac
    31681 agcaaacagt aaaatcctat cagccattgg tggaactgat tcagaatagc gataggcttg
    31741 atattcaaga ggtggcaggg ctgtttcact acaataacca atcctttgag ttttatattg
    31801 aagataaaga gggaagcgta ctctatgcca caccaaatgc caatacatca aatagtttta
    31861 gacccgactt tctttatgtg gtacatagag atgataatat ttcgattgtt gctcaaagca
    31921 aggcaggtgt gggattgctt tatcaagggc tgacaattcg gggaattgtt atgattgcga
    31981 taatggttgt attcagcctt ttatgcgcgt atatctttgc gcggcaaatg acaacgccga
    32041 tcaaagcctt agcggacagt gcgaataaaa tggcaaacct gaaagatgta ccgccgccgc
    32101 tggagcgaaa ggatgagctt ggcgcactgg ctcatgatat gcattccatg tatgtcaggc
    32161 tgaaagaaac catcgcaagg ctggaggatg aaatcgcaag ggaacatgag ttggaggaaa
    32221 cacagcgata tttctttgcg gcagcttctc atgagctaaa aacgcccatc gcggctacaa
    32281 gcgttctgtt ggagggaatg cttgaaaata tcggtgacta caaagatcat tctaagtatc
    32341 tgcgcgaatg catcaaaatg atggataggc agggcaaaat catttccgaa atactggagc
    32401 ttgtcagcct gaatgatggg agaatcgtac ccatagctga accgttggac atagggcgca
    32461 cggttgccga gttgctgccc gattttcaaa ccttggcaga ggcaaacaac cagcggttcg
    32521 tcacagatat tccagccggg caaattgtcc tgtccgatcc gaggctgctc caaaaggcac
    32581 tatccaatgt catattgaat gcagttcaga acacgccgca gggaggcgag gtacggatat
    32641 ggagtgagcc tggtgctgaa aaatgccgcc tttttgtttt gaacatgggc gttcacattg
    32701 atgatactgc gcttccaagg ctgttcaccc cattctatcg cattgatcag gcgcgaagca
    32761 gaaaaagtgg gcgaaacggt ttaggacttg ccatcgtaca aaaaacgctg gatgccatga
    32821 gcctccagta tgcgctggaa aacacctcgg atggcgtttt gttctggctg gatttaccgc
    32881 tcacatcaac attgtaaata tttaaaactt aaatgatttt gaccgacagg tataaccctg
    32941 ccggtctttt tttgctgcta caggaaaact acagactggc tacagggaaa gtacagatgc
    33001 gcttgccata ataacaatcg taccagccac aaatcgtagt tttattgcaa aggaggcatt
    33061 cgatcaaatg gaaaaaagca actatcattc caatgcggat catcacaaac ggcatatgaa
    33121 acaatccgtg gaaaagcggg cttttctatg tgcgttcatt atttcgttca cagtctgcac
    33181 gctgtttttg gggcggagac tggcttccgt attggaggca acacagatac cgcccatccc
    33241 tgcaactcat acaggcagca gcactgacgt agtggagaat ttggaggaaa acgctcttgc
    33301 caccgccaaa gaacagggag atgaacagga atggagcctg attttagtga acaggcagaa
    33361 ccccatcccc gcacagtacg atgtggagct tgagcaacta tcaaatggtg agcggataga
    33421 tattcggatt tctccctatc ttcaagattt gtttgatgcc gcaagaactg atggagttta
    33481 cccgattgtc gcatccggat accgaacaac agaaaaacag caagaaatta tggatgaaaa
    33541 aattgccgaa tataaggcga aaggctacac ctctgcacag gctaaagcgg aagcagaaac
    33601 ttgggtggcc gtgccgggaa cgagcgagca tcagcttggt cttgctgtgg atatcaatgc
    33661 ggacggaatt cattcaacag gcaacgaggt ttatagatgg ctggatgaaa acagctatcg
    33721 ttttggtttt attcgtcgct acccaccaga caagacagag ataaccggtg tgagcaacga
    33781 gccgtggcat taccgatatg ttggcatcga agctgccaca gagatgtaca accaaggggt
    33841 ttgccttgag gaatatttaa aaccagaaaa atgagaaaag gatgttatgg acagaaaaag
    33901 attgacacag cgtttcccgt tcctgcttcc aatgagacgg gcgcagagaa aaatgtgctt
    33961 ttatgcggga atgagatttg acggctgtcg ctatgcgcag acgatagggg aaaaatcgct
    34021 ttcccatttg ctctttgaaa cggattgcgc attatacaac cacaataccg gatttgacat
    34081 gatataccag gaaaacaagg tgttcaactt aaagctggca gcaaagacct taaacggctt
    34141 attgataaga ccgggggaaa ccttttcttt ctggtggctg gtacgccatg cggacaaaga
    34201 taccccctat aaagacggcc ttacggtgac caatggtaaa ctcaccacca tgtcgggcgg
    34261 cggtatgtgc cagatgagca atttactatt ttggatgttc ctgcatacgc cattgacaat
    34321 tatccagcgc agaggtcacg aagtaaagga gtttccagag ccaaacagtg acgagattaa
    34381 aggggtggat gcaaccattt cagagggctg gattgattta aaagtgcgaa acgataccga
    34441 ctgcacctac caaatatgag tgaccttaga tgatgagaaa atcatcggtc aggtgtccgc
    34501 cgacaaagag ccccaagcat tatacaaaat tacaaatggc agtatccagt atgtccgtga
    34561 aagtggcggg atttatgaat atgcccaggt taaacggatg caagttgcct taggtaccgg
    34621 ggaaataata gattgcaagc tgctttatac aaacaaatgc aaaatctgtt atcccctccc
    34681 ggaaagtgtg gatattcagt ctaatggtga taagtcaaga tgaaatttaa gattttcttt
    34741 attgagtaat atgttttgaa gcgcacgaaa ctagacccga tcaaaatcaa atttttttcg
    34801 ggttgattca aggatttgcg tcaataacgg attgttattt ctctatcaca ctccttggtg
    34861 gcgtatagag ttggtgttta cgtagtagcg tatccaccag acgcgtaaat tttcttgcgg
    34921 ttaggacgag tgcacgtttg tgttgatgct ttggaacctc cttatattta ctttgataaa
    34981 aagctttgta ttcaggaaga taatttttta cagagttggc agcttcaact aagtagtatc
    35041 taaaatatcg atttccttgt tttgtcagag gtgtattttg cgactgatag tttccagatt
    35101 ggttcacttt ccaacttaat ccagcgtatt ttgccaattt tgtctgatct tcaaatcttt
    35161 cgatttgacc aatttcagcg attaaaccgg cagcatagac tttaccaacg cctggaatac
    35221 tggttaagca ttgatattct ggaatgacaa caattacttg ttcgatggct ttatctaatt
    35281 ctttgatatt tttttcaagt gctcgtattt ctcgtacgag aacacttaaa acaacattaa
    35341 ttgattcttg tgcaagagca cctaggcggt aactcatggt aattgcacgt tgaattgctt
    35401 tagcgatttt ttctggttgc ttgaaacggc cttttccttt ttcttgaagt aaatcacaga
    35461 aagcttctaa aggaagctca gcaagttcat ccaatgtgaa atcttccgtc atgagtgaaa
    35521 tgagggttgc actaaaaaga ctcgtcgatt cgtctcgcat ttctcgagca agcgtattac
    35581 atttgtacgt tagattttca agaaaatgtt gctttgtacg aatcaattgc ttaataagtt
    35641 gatagcgagt acgtgttaaa cgctgtaagg ccatgtattt ctcttctttg ataggggaag
    35701 ttgtgaatcg ttgaatacgc aagaaatcag cgattcttaa tgcatcgttt cgatcagttt
    35761 tattctcatc aaacatgcga ctaaattgtt tcacccgaaa aggattttca atcgttacaa
    35821 gtgtgttgag cttctttagt ttctcatctt catgaaaaaa catggaagga tggaagctgt
    35881 acatggacgt tgactccatg ccaatcacaa
//
LOCUS       DAGCWU010000011        35384 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.011, whole genome shotgun sequence.
ACCESSION   DAGCWU010000011 DAGCWU010000000
VERSION     DAGCWU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.011"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(97..1116)
                     /locus_tag="MBZ71_000434"
     CDS             complement(97..1116)
                     /locus_tag="MBZ71_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4428412.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1138..2145)
                     /locus_tag="MBZ71_000435"
     CDS             complement(1138..2145)
                     /locus_tag="MBZ71_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBT4428413.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="MBZ71_000436"
     CDS             complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="MBZ71_000436"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBT4428414.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2796..5090)
                     /locus_tag="MBZ71_000437"
     CDS             complement(2796..5090)
                     /locus_tag="MBZ71_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBT4428415.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5350..7515
                     /locus_tag="MBZ71_000438"
     CDS             5350..7515
                     /locus_tag="MBZ71_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4428416.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7819..8652
                     /locus_tag="MBZ71_000439"
     CDS             7819..8652
                     /locus_tag="MBZ71_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBT4428417.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8754..10454)
                     /locus_tag="MBZ71_000440"
     CDS             complement(8754..10454)
                     /locus_tag="MBZ71_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428418.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10961..12121
                     /locus_tag="MBZ71_000441"
     CDS             10961..12121
                     /locus_tag="MBZ71_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4428419.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12137..12580
                     /locus_tag="MBZ71_000442"
     CDS             12137..12580
                     /locus_tag="MBZ71_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4428420.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13063..13593)
                     /locus_tag="MBZ71_000443"
     CDS             complement(13063..13593)
                     /locus_tag="MBZ71_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBT4428421.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13698..14249
                     /locus_tag="MBZ71_000444"
     CDS             13698..14249
                     /locus_tag="MBZ71_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4428422.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14402..14875)
                     /locus_tag="MBZ71_000445"
     CDS             complement(14402..14875)
                     /locus_tag="MBZ71_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4428423.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15013..16485
                     /locus_tag="MBZ71_000446"
     CDS             15013..16485
                     /locus_tag="MBZ71_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBT4428424.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16482..17192
                     /locus_tag="MBZ71_000447"
     CDS             16482..17192
                     /locus_tag="MBZ71_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBT4428425.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17544..17828
                     /locus_tag="MBZ71_000448"
     CDS             17544..17828
                     /locus_tag="MBZ71_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBT4428426.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18109..18528)
                     /locus_tag="MBZ71_000449"
     CDS             complement(18109..18528)
                     /locus_tag="MBZ71_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBT4428427.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18530..19114)
                     /locus_tag="MBZ71_000450"
     CDS             complement(18530..19114)
                     /locus_tag="MBZ71_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBT4428428.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19161..20636)
                     /locus_tag="MBZ71_000451"
     CDS             complement(19161..20636)
                     /locus_tag="MBZ71_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBT4428429.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20817..21128
                     /locus_tag="MBZ71_000452"
     CDS             20817..21128
                     /locus_tag="MBZ71_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428430.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21243..23579)
                     /locus_tag="MBZ71_000453"
     CDS             complement(21243..23579)
                     /locus_tag="MBZ71_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4428431.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23721..24389
                     /locus_tag="MBZ71_000454"
     CDS             23721..24389
                     /locus_tag="MBZ71_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4428432.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24392..25435
                     /locus_tag="MBZ71_000455"
     CDS             24392..25435
                     /locus_tag="MBZ71_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4428433.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25672..26553)
                     /locus_tag="MBZ71_000456"
     CDS             complement(25672..26553)
                     /locus_tag="MBZ71_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4428434.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26712..27440
                     /locus_tag="MBZ71_000457"
     CDS             26712..27440
                     /locus_tag="MBZ71_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBT4428435.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27596..27913
                     /locus_tag="MBZ71_000458"
     CDS             27596..27913
                     /locus_tag="MBZ71_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4428436.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27906..28514
                     /locus_tag="MBZ71_000459"
     CDS             27906..28514
                     /locus_tag="MBZ71_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBT4428437.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28569..29360
                     /locus_tag="MBZ71_000460"
     CDS             28569..29360
                     /locus_tag="MBZ71_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBT4428438.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29498..30010
                     /locus_tag="MBZ71_000461"
     CDS             29498..30010
                     /locus_tag="MBZ71_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBT4428439.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            30036..30908
                     /locus_tag="MBZ71_000462"
     CDS             30036..30908
                     /locus_tag="MBZ71_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBT4428440.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31084..31446
                     /locus_tag="MBZ71_000463"
     CDS             31084..31446
                     /locus_tag="MBZ71_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4428441.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31455..32207
                     /locus_tag="MBZ71_000464"
     CDS             31455..32207
                     /locus_tag="MBZ71_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428442.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVKPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32221..34023
                     /locus_tag="MBZ71_000465"
     CDS             32221..34023
                     /locus_tag="MBZ71_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428443.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34300..35340
                     /locus_tag="MBZ71_000466"
     CDS             34300..35340
                     /locus_tag="MBZ71_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBT4428444.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT        11053 a         6255 c         7288 g        10788 t
ORIGIN      
        1 tttttacaga taaacaatgt gttagaagta gtatcttctt ttacataaaa agctgaccat
       61 aggataaatg gtcagcgtat ttagtgagtc agctatttag tatgggtttt ctaaaggaac
      121 gactgtctct cttttgatca agcgatgagg gatgaccaaa cgtacgccac tgctcaattc
      181 ttcatggatc aattcgcgca gcttttgcat agcgacacgt cctaattcgg agatatcgat
      241 atcgatactt gtaaggtaag ggtgtgttaa tgttgagaag atcgagttgt tgaagctaat
      301 gacggagagt gtctctggaa tgacatagcc atacatttgc gccaactgca tcgtgcgaag
      361 ggcgaagata tcgtcaatga ccaccaatgc tgtggcttcg ttttctttca acgttttctc
      421 aaaggacaga taatcctccg gatgctctag tactacactg gataacgcag gaagaccagc
      481 catcatcatt gctttttgat aaccgaaata tcgttcgaaa tacaaacttt catgagtcgt
      541 gtttgtcaca aacaggatat tcttatgtcc attctggatc aaaaattctg ttgcttgttt
      601 acctagaagc tggttatcat tatctacata aacgatttcg ttctcatgat gataaggctg
      661 tccgatcaaa gtgaaaggga tgtggttttg gtataaataa tcaatgacag tatcttgact
      721 atctgaatac actagaataa aaccatctac ttgtttttgt aaatgcatac gctggacgtt
      781 ctctaacagc aattcaaaat ttttagcagt agcgattgct gtagtcatat cataattccg
      841 cgcttcttcg ttgattgctt ccatgatctc aagataaaat gggttaccga tccgctcttt
      901 tgaatcaaga gggggtaaga ttacgccgat ggcactggaa atgcgttttc ctagattgcg
      961 ggcagtcatg ttaggaacat aacctagttc atccataatt tttcggactt tcttctttgt
     1021 tgtctcggaa atactaggat gatcgttgat gacacgagag acggtcgagg tggcaacacc
     1081 ggcttttttc gccacatctt ttacggtaat agccatgata aaaacacctt tctcttgtta
     1141 ttgtgcagag aaccgataaa tcgtctgact gatccgttct tgaccaggat ggaggttgat
     1201 cgatccaaat ttggggaaat gaacgatatc agggatttct tgcggttcga ttgctaatcc
     1261 gtagtttgaa tgcatctgtt ttccattgac agagaaatca gcttcgaatc cagtcgtaga
     1321 aaataatacc atacttttat tagaagttgc tatagtcatt tggcgaccag aatgcttctc
     1381 gaataattgt aaggaagttt tggaaagcct aggatgatgc aaaagaaata catcatctag
     1441 tccatctgga taacatgcaa ggatctcttg cagtgttggg gattttctaa agtcgattgg
     1501 cagtttttct ttttttgtcc aatttccagt aggcagtttt tcttgatcta attcaagcac
     1561 accatctaaa aagaccgtta actgatgttc atagatgtct cttgttccat ttcctgaaag
     1621 gttgaagtag gcatggttgg tcggattgca gatggtttca acaaggctac tagcagaggt
     1681 tgtcatctct aattcgtttt ttgttaaacg gtaagtgatc gtagcagtga ttggtcctgg
     1741 ataggtagaa aattcatctt ttaagtaaaa gaccacacct attgatgatg gagttttgaa
     1801 aacttctact tcccaaaatt ggaaagacca gcctttcgtg ccaccgtgga tatggtgtga
     1861 tccgcagttt ttctccaatc tatgctcacc ccaagaagca tcccggatcc ttccggctac
     1921 aggtccgaca gttgctccaa aaaagctttt gtctgttaga acatcttcga aacgatcgta
     1981 agacaataaa acattttctg attttccttc tttgtctggt gcgaagattt ggtgcaggcg
     2041 ggcaccgtaa ttgagaagaa cgacttctaa gtagtcgttc ttcaatgtga ttttctttat
     2101 ttcagtctct ggaactggta cgacggagat ctctggatta tccatggtca tgccactttt
     2161 ctttcaagta atcgatcgta agaaaagtcg tatcagggac aagcgggatc tcctctccaa
     2221 ggtcttcagc acgtcccacg ccaattggtt gagcgccact tgagaggatg gcttggatac
     2281 cggcttgcgc gtcttcgatc ccgatacatt cttctgcttt tagtccgaca gcttttgctg
     2341 caaggaggaa gatatctggc gcaggttttc cgtttgttgc ttctgccggg tcggcaatag
     2401 catcaaagta aggtgtaaga cccatttttt caagtaggaa tggcccgttt ttacttgcag
     2461 aagcaagagc aatcttgatt ttttcttctt ttaaagaatc cagtaaaggc acgaccccgg
     2521 ggaagacatc ttttggttcg atcgtctgga tcatctctaa ataataatcg ttttttcttt
     2581 tcgctaattc tgcaaattct tcctttgaaa aagagtgttc ttttccgcca taagctaaaa
     2641 gcaaagagag tgagtcttct cggctgacgc cttttagctg ttcgttgaat gtgtggtcga
     2701 tcgtaatgcc gatttcctga ccaagctttc gccaagcacg ataatggaat tcagcagtat
     2761 cggtaatgac accgtctaaa tcaaacaata cccctttaaa catttgcttc ctcctttctt
     2821 agcgttattt ctagacgatc cgctaaagaa taagactgtt catagacgta aatttctaga
     2881 ggagaacctt ctaataactc gaaagtaact tgttcatctg tgactgagat gaacaataaa
     2941 cgtccgcgat aattgatatg gaaagaataa gctttccaag tttttggcag aaatggtgcg
     3001 aatctcaact gatcatgggc agttttcatt tgagcaaatc cttggacaat cgacaaccag
     3061 ctaccagtca tggaagtgat gtgcaagccg tcttctgtgt cgttattgta attatccaaa
     3121 tccaagcgag ctgttcgctg gtacatttct acagctttgc tttccattcc taattctgct
     3181 gctaaaatcg aatggatact tggcgaaagg gaagactcat ggacagtcat tggttcatag
     3241 aaatcaaagt ttttttgctt ttcttctttt gtaaaagtat cattgaagaa ataaatcccc
     3301 tgtagtacat ctgcctgctt gatgaagcaa gatctaagga ttttgtccca agaccaatgc
     3361 tgattcagag gaagatcgct tgtgggaata tcggatacag gcatgagatc tttatctaag
     3421 aatgtgtcat gctggacaaa gattcctaat tcttcgtcaa ctgggaagta catgttctct
     3481 gcgatatctt tccatttggc tagttcttct tcgctgatag tgatagcagc ttctttttga
     3541 taagttaaat aattttctcg cgtgtaacgc aagacccaag ctgcgatcgt atttgtatac
     3601 caattgttat tgatattgtt ctcatattcg tttgggccgg tcacaccgtg gatcatgtat
     3661 ttatttgctc gttttgaaaa atggacacgg tctgcccaga agcgtgcgat ttctgctaaa
     3721 acttccagtc cttcattttt caaatattcc gtatcgccgg tgtaattcgt ataattgtag
     3781 attgcatagg caatcgcgcc atttcggtga atctcctcga atgtgatttc ccactcgtta
     3841 tggcattcga caccagtgaa ggtgaccata ggataaagcg cgccttttaa tccttgttgc
     3901 tgagcgttgt ggattgcttg cggcagttga ttatgccgat atttcagcag attcttagtt
     3961 acttcaggtt tggctaaagc aagataaaga gggacagcat acgcttctgt atcccagtaa
     4021 gtggctccgc cgtatttttc tcctgtgaat cctttggggc cgatattcaa tcgttcatct
     4081 tcgccgtagt aagtagagaa tagttggaac aaattgaatc gaataccttg ttgtgcttct
     4141 tcgtctcctt cgatgaccac atcggcaagc ttccatcgtt ctgcccaagc cgctgtttgc
     4201 tcttcttttg cttggatata attgtttgtc tggttcgtta attctgagac agctttttct
     4261 gcttgctgcg cttctggaat atctctacta gtaagaacga taatttcttt ttccactgac
     4321 catgtctcac cttgagataa ttctgcagac aagcaaccat gtaatgctaa agtatcttcg
     4381 tgatagtcag ggatgactga ttttccatta acgaaatggc gcattgctgc agtaacagta
     4441 aattgttcaa tcccaaagtt gtttggaata gttttagtcg tcaaatagcc gatttcttct
     4501 atagttccac gatcaatctc attccaaaaa tgttcatcat agttgctgtc ttcgttttga
     4561 acatttccat ccaattttga tacgagtttg actgttccgc taccttcaag catttctata
     4621 gttaaataga tataagctga ttcttttttg acaatgctca gaaaacgttc gaaggaaatg
     4681 cgtactttat tagtagaagt agtgattgtg aatgtacgag ataatacacc atttttcata
     4741 tctaattccc agtaaaagtc ttctggttcc aaagaagcca agtcgatcag ctgatcgttg
     4801 atcgaaatat ctatcgcgat aaagttcaag gcgttgatca cttttccaaa gtattctgga
     4861 taaccatttt tccaccagcc gacacgtgtc ttatcgggat accagacacc cgccaaataa
     4921 gtaccttgat ggtggtcccc tgagtaagtt tcttcaaagt tcccgcgcat ccccatataa
     4981 ccgtttccaa tacttgttaa agattcttgc agtcgaagat tttttttatc taattgtgtc
     5041 gtgctgattt tccatggatc aatagcaaat agtcgtttga tctgtttcat ttttctcctc
     5101 ctaatgagct gttttatacg ttcataataa acgcaaccga ttgcacaagt caatagacaa
     5161 aagaaaatat ttcatagaaa aaagcttaaa aaaataaatt tttaggagaa aattatagaa
     5221 gggaacacac gtattatagg ttttatagaa tggtatagac agaaaaataa aaaataaagc
     5281 atttacaaaa aagaaaacgc acgctataat acgtttaacg aaaccgattg cgtaaaggag
     5341 ctaaacggaa tgaaaaagat atttagtttt gaattttggc aaaaatttgg aaaggcttta
     5401 atggttgtgg tagcagttat gccggcagct gggttgatga tcagtatagg gaaatcaatt
     5461 cccttgatca atcctgattg gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt
     5521 ggttgggcaa ttattggtaa tcttcatatt ttatttgctt tagcaatagg aggaagttgg
     5581 gcaaaagaaa gagcaggcgg agcatttgca gctgggatct catttatttt aatcaatcgg
     5641 atcactgggg ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc
     5701 cacactctgt tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt attggaagca
     5761 ccagctttga atatgggagt attcgtaggg atcatcgctg gatttgtcgg ggcaatggct
     5821 tataataaat actataatta tagaaaattg ccggatgctt tgtcattttt caatggaaag
     5881 cgctttgttc cgtttgttgt tatcttatgg tcaacaatcg tggcattgat tcttgctgtg
     5941 gtgtggccaa acgtacaggc tggaatcaat aattttggtt tatggattgc agaatcacaa
     6001 gaaagcgcac cgattctagc accgttttta tttggaacat tagaacgttt actattgcca
     6061 tttggattgc atcatatgct gacgattcct atcaactata cacagcttgg cggatcgtat
     6121 gagattttat caggcgcaca ggcggggaca caagtatttg ggcaagatcc gttatggttg
     6181 gcttgggcca ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca atatgagttt
     6241 gtgttgcata actggacgcc ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt
     6301 atcttgatgg gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga
     6361 tataaatcaa tgtatttttc agcagcgttg gctgtctttc taacaggtgt aacagaacca
     6421 ttggagttca tgtttatgtt tgctgctgtg ccattatatg tcatttattc agtgatccaa
     6481 ggggctgcat ttgcgatggc agatatcttg ccattgcggg tccattcatt tgggaatatc
     6541 gaattgctga ctcggacacc gttagctatc aaagcgggtc taggtggaga tcttatcaac
     6601 ttcgtgttgt gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc
     6661 aaaaaattca actttgctac accgggacgt aacggaaatt acgacaatga cagtgaagaa
     6721 accgttgtat ctaacagcgg aacaggaaca gcagatcagc aagtggtcca gatcattcat
     6781 ttgctgggag gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg gttacgcgta
     6841 agtgtaaatg atcgagaaaa agtagggact gaagaagcct ggaaacgagc aggggcgatg
     6901 ggattgatcg tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc agatgtcttg
     6961 aaatcagata tcgaagatct gctgcaatca ggagcagaga ttccagagcc agaaatggtt
     7021 tctacaggaa atggacaagc aggtagtaag caatacttag gtatagaaca agaactagtt
     7081 tccgcagctt ctggagaagt gattccttta tctgaagtaa aagatccggt attctcacaa
     7141 aaaatgatgg gtgacggctt tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc
     7201 gctggaaaag tgacaagtat tttccctagc aaacatgcaa ttgggctgga aacgaaagat
     7261 ggtatcgaag ttttgatcca tatgggaatc gatacagtcc aaatgaatca gccagctttt
     7321 gagattgctg tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc tgagatggat
     7381 cttgatgtga tccaaaacga agaaaaagat ccaacgatca tgatcgtatt tacaaacgat
     7441 aaagtagaag aagtagtgat caagcaatta ggtacagtga cagctggtaa agtgatcggc
     7501 tccattaaaa tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag
     7561 ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg agaagcagca
     7621 gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa
     7681 cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga
     7741 acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg
     7801 aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca
     7861 gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg
     7921 attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta
     7981 atgaagttct gtccggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt
     8041 ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac
     8101 atcggttatg ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt
     8161 caggtcctta ccgtctccca atttcaaaag aaagaagatt atcagacacg caagatcttg
     8221 attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg
     8281 tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg
     8341 gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt
     8401 tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa
     8461 aaaggaaaag cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa
     8521 ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt
     8581 tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat
     8641 gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca
     8701 aacgattata ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca
     8761 cgtaaaagct tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa
     8821 gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg
     8881 attccttttt gctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat
     8941 gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt
     9001 ttgtccccag tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa
     9061 gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc
     9121 ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata
     9181 tctttgctga agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga
     9241 ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt
     9301 gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac
     9361 tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga
     9421 aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga
     9481 atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat
     9541 aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga
     9601 aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt
     9661 tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc
     9721 caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct
     9781 tttccttttt cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct
     9841 aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg
     9901 ataacaggga actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa
     9961 aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta
    10021 ctcatgaata aaacggctac tggtacactt acccaatttc gaacattgac atatctcagt
    10081 aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa
    10141 tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg
    10201 taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg
    10261 aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca
    10321 tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg
    10381 aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta
    10441 gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ctcatgaaaa
    10501 tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat
    10561 aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta
    10621 gctttttcat gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca
    10681 ctagcctaga aaatatgaga agctattttc tgactagcgc tcttattact caaagcataa
    10741 cgcagttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    10801 cccaacccaa caaaaaatat gagtagctat ttttttgggc tgtacttatt acttgatgca
    10861 ggacagcttt ttcacaacct catcggcttt cattttctat ttttttcctg aacccatata
    10921 ataaaagaaa gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa
    10981 acagtctcgt cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg tagggttact
    11041 gatcgcttta tatccatttt atgtggattc tttaaatagt tttattgatc aaaaacgtat
    11101 cgaacaagta caaaaacgga ccgcagctga aattgaagtg cagcgaaaga aaatggagga
    11161 gaaaaaccaa cgattaactg accaaggatt caatcctggt actgatcctt tcaatgaaaa
    11221 gaacagaaat gaaagtacaa catccagtca attagaggaa tggctgatcg gaagtatcaa
    11281 tatacctaag atccaattaa atatttcact ttatgatcgg ctcaatggta ggatccttga
    11341 aaatggagca ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt cgacgcattc
    11401 agtgatctct gctcacagcg gtttacctaa tcgacggtta tttaccgaat tagatcgttt
    11461 agaaaacgga gacacgttta ttttgactgt tttgggggag aaattggctt atcaagttaa
    11521 aaatatacaa gtggtactgc cagatgacac gtctgttcta acgatttcag aaggagaaga
    11581 cttagttacc ttgcttacat gtacgccgta tatgatcaac acgcatcgtt tgctagtgac
    11641 aggacatcgg attccttata acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga
    11701 aagaaccatg cggcaatggt ttattcttgc aggaacgata atagctgtag tcatactgtt
    11761 agtgtttatc ggacgactaa tctatcagta tagattaagt aaaaaagtgc ttgatttttc
    11821 atttattatt agtgattcgt cagggaaacc ggtaaatggt agttcattca ttttgaaaca
    11881 taagaaaaaa acacttacta gaaatggtgt gccgttttca gtacaaagcg accatcatgg
    11941 gaaagtcaag ctggatcaat tgcctggtgg aacttatcga atcgtttcag ctgatgatcc
    12001 taaagttgct gtttcttttg gtattagaaa agtaaaacaa gagaagatat acttttttga
    12061 ggggcgaaaa cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa tcaatgaata
    12121 agtaaggaga gaaaaaatga tccgcacaac aacaaaattg tctgccaaag aactggaatc
    12181 tgttctatcg atttggctgc atgctaactt ggattctcat gaattcattc catcaactta
    12241 ttgggagaat caagtagaat ctgttaggaa agctttacca gatgcagtga tttacttagc
    12301 agaaatagat caagaaatta ttggttttgc tggggtgatt gatcagttta tcgcaggaat
    12361 ctttgtgaaa agggaatacc ggcaacaaag aatcggtacg caactaatga aaaaaataaa
    12421 agaaaccaat aaaacactga ctttgacagt ctatcaaaaa aatcaatcag ctattcattt
    12481 ctatctttca caaggtttta caattcaaac acagcaaaaa gatgaagcag taaatgagga
    12541 ggaacttgtg atgaaatgga atcaaaacgc tgatttttga tgaacagaat aacgaaataa
    12601 tgctcccagc ctaatttttt gctgggagca ttatttcatt ttgaagtcac taatttaact
    12661 gtgtgaatat gcagttgtta caatctttga taaacggtgt tcaatcagct gaccctcggt
    12721 attagctcaa aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc
    12781 aggtcaaaac gctgatttca caacctcttg ataaacggtg ttcaaacggc tgcttctcgg
    12841 taataagccc actagcctag aaaatatgaa aagctatttt ctaactagcg ttcttattac
    12901 tcaaagcata acgctgattt cacaacctct tgataaacgg tgttcaatca gctgaccctc
    12961 ggtattagct caaaaaacgg caaaacatga ggaactattt ttccgtttct ttctctaata
    13021 ctcaggtcag aacgctgatt tcacaacctc tctttcacaa ccttatttta ctcgtttata
    13081 attttcatcg aagtattttc cgctacggat atcatctggc attccatcgt atggttcttc
    13141 gctaataaca tcttcattgt ctatacctgc atcacccata taagtgatcg tagcaaattc
    13201 aggaacaagt aattttggat aagtttcata tttttcacga gctgcttcca tttctgcgat
    13261 atgttcgttt tggaaagtaa cagtgatttc tggatgttct tcaacccatt tagggaaaaa
    13321 gatcattccg tgaagtttct tttcatttgg cataaagtcc aatgccacgt ctgtccctgt
    13381 tggctctgtc caagtgattt tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg
    13441 gtcttttacc caacggcctt tgaccattcc gccatggata cggtaatcaa cagtatggtc
    13501 atttttggca taccattcat attcccaacc attgtcatac gtatagataa agtgagttcc
    13561 aataaaatct tccaattctt taaattcttt catgtttaag catctcctta taaattaaaa
    13621 catgtaataa actacatgtt ttcgattact catttatgat agaatggatt taaagatttg
    13681 tcaaggaggg aaaactgatg gcaagaaaaa atacacttcg atatattttg ttagggttat
    13741 taagtaagaa gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat gaaataggag
    13801 aattttggca agcaaaacat agtcagattt atcctgaact tgcaaaaatg gaagagcaag
    13861 gaatcattca gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat
    13921 taacagaaga agggaaggaa caattggcag agtggattca tacacccacc aatgaactac
    13981 cggttaatcg agacgaattt gttctgaaat tgtatttcgt caaagacgtg aatgatccgg
    14041 cattagtaga aattattcag cagcagcgtg accttcatga agaaaaacga cttcatctat
    14101 tagagcgaaa aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat tacggacact
    14161 acctgatttt agcgcatgcg atcaatcggg aaacagaata tgcaaattgg ctaacagatg
    14221 tattagaaga aagtaaccga aaaaaataaa aaaatatccg aacagtgatg gagaattcct
    14281 ttctatatga aagaaacgca gatcaccgtt cggatattct tatgttcaaa caggcctttt
    14341 ttcaagtatt tatgtagcat ctacttatta aaaagcttgg tacttaggag attagtggcg
    14401 tttagagttc ttttaacaac tgttcgtagt cagctttttc atcgacagga aggagcttgt
    14461 aagttaattc gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc tcttctttga
    14521 agttaccgat ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt
    14581 ttgaaaaaac tttacggaca tggtgcattt tcttttggcg gatttctgcc ttttctgcag
    14641 cttttgacaa agtaaatccg tcatctaaat attttttgat cagttctact tttatgactg
    14701 taggtaaacg atattttcta ggacaattcg catcagcttc gatcgaacga atgaatccct
    14761 tttgttccca ataccttaat tgacgtggag acacacctac gatatcactt aattcactga
    14821 tgccaacaac caattgatca ttttccaaca aacgtttaag ggaagcccca accatttttg
    14881 tcactccttt tgtcctcttg cttcccttaa ttatatccgt attaacttat gtgtcaagta
    14941 ggttgacaaa tagcgaattt aggactaaag tattctagta gtttcagaaa aaattcagaa
    15001 agaaggagaa atatgagtaa caaccaaccc gtagatattt acggaaaacc atataatcgg
    15061 acacttttag ttgttgtctt attaattggt acattttgta ccgttttgaa tcagacctta
    15121 ttgacgacag cttttcctac attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa
    15181 tggttaacga ctggtttctt gctagtcaac gggatcatga tcccaataac agcatggctg
    15241 atcaataaat tcagttcaag aaatttatat ctttctgcca tgacgatctt tttgatcgga
    15301 acgattactt gctttacagc acctaatttt agtacattac taatcggtcg tttgatccaa
    15361 gcttgcggtg tgggagtatc gatgccatta ttgcaaaata tcatgctatc tattttcccg
    15421 ccagaaaaaa gaggatcagc aatggggatg tcagggatcg ttattggact tgctcctgct
    15481 ttaggcccta cattatctgg ttatatcatt gataactacc attggcgtga cttgtttggt
    15541 atggtactgc caatcgttgt tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt
    15601 attccattgt caaatcctag cattgatatt ttatccgcta ttttatcaac gattggattc
    15661 ggtagtttac tttacggatt ttctagtgta ggagatgacg gctggggaag cttgaaagta
    15721 attggtttct tagttgttgg tgtgattttc atcgccttgt tctcatggcg tcaattacat
    15781 ttggaacatc cattcttgga acttcgtgta ttcaaatcat ctacattcac gattgcagct
    15841 gttttagctg gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt accattgtat
    15901 attcaaaata ttcgtggaga atcagcattc cattctggct tgatgttgtt gccgggtgca
    15961 ttgatcatgg gggctatgat gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa
    16021 cgtttagcca tcacagggat gttcattttg acagcggcaa ctgcaccatt tgctttcttg
    16081 acaaaaacga ctccagtttt atatatcgtg atcctttatg cgatccgtat gtttgggatc
    16141 tccatggtca tgatgccggt aacgacttct ggtatgaatg cattgccaaa taacttgatc
    16201 agtcatggga cagcggtaaa taatacattc cgtcaaatcg caagctcgat tggtacagct
    16261 attttgatca gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa atctgtattg
    16321 gataatacgc cattagctta taaagatcag gcaatcaatg ccacactgtc aggttaccat
    16381 gccgcgttta tcgtagcgat catctttggg attcttggct ttattattgc ctttttattg
    16441 aagaaaaaag aacctgttct aatgaaagcg ggggataatg catgattatt ggagttttga
    16501 ttgtcagctt gcttgctttt gctttaacga acgtgttcgc taaaaaaaca tggcaaacct
    16561 ttttatcttt gattttcgga ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt
    16621 tgtccaatca ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca
    16681 gtgctgattc ccaaggagca gatatgcttt tgtataaagc tttagggaat ggaaaagaaa
    16741 aagtgtatct ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact ggtacggata
    16801 acgaaacaaa taaagtagaa aagacagacg gcgatgctga aaaagtgacg aaaactactt
    16861 attgggaata caaaaacgac atgtataaat tctggttcaa tattgctgat aacaatcacg
    16921 aatatgacag ccgagtgaat acattcaaaa tcccagagac atgggtagaa ttaagtacgg
    16981 accaagcagc caagttagct gaattagtga agaaacagca aagtacgatg gaaagtgaag
    17041 caaaagctta tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca
    17101 aagcagagca agaacaacgt acgaaagcct tagctgctga attccaacaa caagcattcg
    17161 caaaattagt gaaagaagca aaaggcgaat aatattttga acaccatgta ggaggttgtg
    17221 aaaaagctgc tctgcatcaa gcagtaagaa caatcagata aaaatagcac tccatatttt
    17281 tcatctgatt gggcttagtg ccgtagatgc aagcttttga acaccgttta aggaagaggt
    17341 tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca gcttctcatg
    17401 tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcgaaa
    17461 aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat gctatactag
    17521 atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca aaaatctatg
    17581 cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata agaaaagaat
    17641 tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat gaaggcagta
    17701 tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt ggactacgaa
    17761 aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt acagtgaagc
    17821 aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac ggtgttcaaa
    17881 agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca tgtttacaaa
    17941 atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa acggcgttca
    18001 atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta tttttccatt
    18061 tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc ataatggttt
    18121 ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt ccaaccaatc
    18181 taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt gtgcattgga
    18241 gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg cagttttgat
    18301 gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat cgtggctagg
    18361 gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc cgatatgtgg
    18421 tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa cagcagcttg
    18481 gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat taatcaccct
    18541 gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc attgtatggt
    18601 gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt ggtggtatga
    18661 tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca tttaatggcg
    18721 tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca gcagcacgtg
    18781 caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata cttgccggta
    18841 cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct aaatctttgt
    18901 ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg gattccagct
    18961 ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc ggatattctt
    19021 ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt acaccaatca
    19081 cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt tatgatgtca
    19141 aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac ttcttggaat
    19201 tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa gatagttttt
    19261 gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc tgatgggtcc
    19321 aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg gaaacgagtg
    19381 ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc gactaaaatg
    19441 actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg ctgaatacct
    19501 tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc accacctgca
    19561 ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa ggcaagaata
    19621 cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc aatcgttgtt
    19681 tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac ttgtacagct
    19741 ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga gttcgtgttg
    19801 atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa ttcagcacga
    19861 gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc gttacgcaaa
    19921 gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc gaaagtcgtg
    19981 ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg tttattcaag
    20041 gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat gtacataccg
    20101 atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat tgttttttct
    20161 ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc aggagtattt
    20221 gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag atgaacgact
    20281 tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt ttctacagcg
    20341 tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc tacacgttta
    20401 cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat attgttgaaa
    20461 gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc gatataacgg
    20521 taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg atattgacct
    20581 ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc tgtcatgaga
    20641 gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag gaaaacaatt
    20701 caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat aatagaataa
    20761 actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga aggaaagtga
    20821 aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg aatattttga
    20881 aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca gaagagataa
    20941 ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt ctcgcacttt
    21001 ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca gtattcataa
    21061 atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa agtataccag
    21121 aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac ttcctcgttt
    21181 cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg gttgtcacaa
    21241 ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg tgcaggaaga
    21301 tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc atggacagga
    21361 tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt gatagggaac
    21421 gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa aatacacgtt
    21481 tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt tagtacaccg
    21541 atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat gatcatactt
    21601 gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc catcaatccg
    21661 ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc ttcatctgat
    21721 ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc aaaattgttc
    21781 ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag caatgtttgt
    21841 ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc tctttgagct
    21901 ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg atcgctataa
    21961 gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag gatccccgag
    22021 atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt agggataaaa
    22081 ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa tgttgtacca
    22141 acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag attcttcaat
    22201 gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt tgctgaggct
    22261 ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt gctagtgtca
    22321 agctgtgtag ggaaacttga cactcccaca cctgtcatcg ctgacatcgt actagataaa
    22381 gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac tttgccattt
    22441 acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt acttaagtta
    22501 ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt gtgctgtgct
    22561 ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc cgctttgctt
    22621 gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat agtaatcgga
    22681 aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc agaagaaagg
    22741 gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt caagaaagta
    22801 cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca aaaactcatc
    22861 tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg atgggaatct
    22921 gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg ggccagttca
    22981 ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat caattccatg
    23041 atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa aaggatatct
    23101 gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc agataactga
    23161 tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc ttctgcccgt
    23221 tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac gttttcaata
    23281 atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga gttgtttcgg
    23341 taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat taccatatca
    23401 ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt accagaacca
    23461 ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac accatccaat
    23521 gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa ttgaagcata
    23581 atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag aacaatgatc
    23641 gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct agaaattttt
    23701 caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat ccgcaattac
    23761 agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc gcattgaatg
    23821 gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt acagatatca
    23881 tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc agatttgaca
    23941 cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc gggttcaaat
    24001 taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc ctacgtgtgg
    24061 aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc ggacaaacaa
    24121 aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt ctgcctcaaa
    24181 aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc actcgccaac
    24241 agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg atcgatgtac
    24301 atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc atcacagtac
    24361 ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt cacaattatg
    24421 gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt gttttctcgg
    24481 ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg gaaaaccaac
    24541 gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag gaatatcgat
    24601 ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata tgagtctggt
    24661 agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag aagtacagca
    24721 actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga cactgaggaa
    24781 tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa tccaaggata
    24841 tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat ttttatatcg
    24901 gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa aattgaacaa
    24961 actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg aacaaatcag
    25021 agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat tggatatcga
    25081 acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt ggctgaatat
    25141 catggataac gccataaagt acaatcaaat caatggacag attcatatca aactatttga
    25201 gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga atgaagagac
    25261 cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa tttctgggaa
    25321 tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac gtattgatta
    25381 tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac aataggaaaa
    25441 agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc gtttttttgt
    25501 gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc ttattgaact
    25561 acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga gagcgaaagt
    25621 catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg cttagttttc
    25681 ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac cgactttacg
    25741 agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag tattttcttt
    25801 taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat ttgcaatcat
    25861 attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa atccagcttc
    25921 gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga gcaaaaagtt
    25981 ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat gatttttagg
    26041 tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac ttggtggggc
    26101 ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat tgtaagttaa
    26161 ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt tcaaaaactc
    26221 ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga tatttaatga
    26281 aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt attgttctac
    26341 gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga tttttctatg
    26401 ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct gtttagaaat
    26461 atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg tttcggctaa
    26521 atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta ttccaataaa
    26581 gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac aatctttctt
    26641 gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta gaaaaagaga
    26701 ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg tttggcggac
    26761 taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag ctgacgatgg
    26821 tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg aaaaatatgc
    26881 cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt catttaggta
    26941 atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc ttccctagtg
    27001 aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc attgttctat
    27061 tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca ggagacagta
    27121 cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt gcgtgtaatt
    27181 tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat attttgttct
    27241 tattggcaaa atttatttat ccattgacta caccagacat gatcttagat tttaaagctt
    27301 gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa atgttcccag
    27361 tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg tggacaaatt
    27421 ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg actaaaaagg
    27481 atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac tatattatat
    27541 gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg acgtaatgca
    27601 catccagatt ccaactgttc ttcttgatgg tactgtgtta gcagcattaa gcaaggaaga
    27661 tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca gtgaatctac
    27721 actatatccg gttttgagaa ggttgaaaaa agaaggatat cttgagacct atgatgaacc
    27781 ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc gtcatttaga
    27841 agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat tggaggagag
    27901 cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt cctgaagacg
    27961 atcgttttga tttgatccag tattatgagg aatatttttt agattctgga aaaacattag
    28021 atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta aaaatcagtt
    28081 acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa agacaaattc
    28141 gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt ccactagcac
    28201 tggggtttat attggttatt gggggactat tggttgcttt actagcgctc atttttagta
    28261 tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc tcaggaatca
    28321 gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt ctgttcgcca
    28381 caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg cttttttatg
    28441 tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga gatctgcatt
    28501 cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg attaccggta
    28561 ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta ggcgctcaaa
    28621 agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag acaaaaaaaa
    28681 tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg gtcatacgcg
    28741 gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa ataaaggaga
    28801 aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc attagtttag
    28861 aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta tctgaattga
    28921 caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa aaagtgaata
    28981 ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca ataactgcga
    29041 aagatgaatt aggctcactt cgactagatg aggccaatgt ggataacttg atcgtcacag
    29101 gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga aaagtcatcc
    29161 taaacggtgg taatttatct ttgcatgaga tggaaaacag tggctatact atccaaacgt
    29221 ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg ttagaacaag
    29281 gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg acggatcaag
    29341 aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa aacagcaaag
    29401 atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt aaaataagaa
    29461 agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag aaggtcaaag
    29521 agaaaagtta aagacagaaa tggaaagatt acatacttat atcactcaac taagccaaac
    29581 cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt cagaaggtcg
    29641 ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga agaaagagct
    29701 ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg ggaagaagga
    29761 acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga tggatctaga
    29821 ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa aattggatta
    29881 tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg aagacaaaga
    29941 gttagaacta gaaggtgtaa tggcgcgcat tcgaaaaaaa attaaaaaag taacattaga
    30001 tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga gcattttata
    30061 tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga ttgtttttct
    30121 tctatttagg catgtggcaa gcgaaagttt gggcactttt tttaagtatt tttctttggt
    30181 tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa gcaaaagtaa
    30241 ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc cttacgatac
    30301 ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt tctgttttaa
    30361 aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc tttaaagtcg
    30421 ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta gaaatccaaa
    30481 gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc aatgagaatg
    30541 atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa gagctgcaag
    30601 agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat ttagcctatg
    30661 caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt ttgtctgctc
    30721 gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag caaatcgaag
    30781 atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat aatttacttg
    30841 tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac atgaaagacc
    30901 gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt attgtcttga
    30961 actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg atagttgatt
    31021 cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag agaaggggaa
    31081 aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact tgcaggggtg
    31141 aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat tacgacagat
    31201 ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta tagatatatc
    31261 ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg acaattgact
    31321 cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa aggtgtcact
    31381 tcgtatgaag aagtcgaaaa aaaagacgtt ccgaaaacag cactggaaaa actaattaaa
    31441 gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa gtatacggaa
    31501 aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta gaaaaaggcg
    31561 aatttattgg gatcatgggc gcttctggtt ctggtaaatc gacacttttg aatattttat
    31621 ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat gtgaccacat
    31681 taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt ttccaagact
    31741 tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt tctttacaag
    31801 gcgtcaaacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt ttatccatct
    31861 ctcatatttt ggaaagctat ccttctgaga tttctggtgg acaaaaacaa cgggtcgccg
    31921 ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct acaggtgcgc
    31981 tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat acaaaagatc
    32041 atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc caacgtattt
    32101 tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa agccgtgatt
    32161 ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg gagattgact
    32221 atgctttgga aattatcact tactgggatc aagagccgtt taagagacta cctggtgtta
    32281 ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat ggcaagcaac
    32341 gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt ccgattcggt
    32401 tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc ctttttgatg
    32461 acgatgcggc aaaaagacta tgcaatgttt atgatgctag gagcaaaagg cagaaaaatc
    32521 gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt agtgggttca
    32581 gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca gttagatctc
    32641 aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt tttgtttttt
    32701 gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa aaaaccgata
    32761 ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa attcttgttt
    32821 ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat gatgaaacac
    32881 gtggggacct atcaagtctt tggtatagga gtagccttag ttacgatcgt attaggaact
    32941 tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa atcagaacag
    33001 atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg tattcgtgaa
    33061 tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg cgctttgacg
    33121 gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc tggatatgat
    33181 ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact ttcaccaaca
    33241 ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa tgcaagtgaa
    33301 tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc gaagatcacg
    33361 aaagaaaaag ttcctgctga aaaaatgaat gaactgactg tacaagagga acttcgaagc
    33421 tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc agaatttgat
    33481 caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt ccaagcaaac
    33541 ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc tttgcaaaaa
    33601 gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct attctctggt
    33661 tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc tagctgtttg
    33721 atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat gcttgaaaag
    33781 ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg cgtgttattc
    33841 ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat gtttggtcta
    33901 ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt cgtattgtat
    33961 ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa aaagagattg
    34021 tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc tcctcctatt
    34081 tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt tgttcggaaa
    34141 aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta ttagaatata
    34201 gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc catgtatact
    34261 ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa tgaacaaaat
    34321 tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc tttgtctgtc
    34381 agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga aaaacgagag
    34441 ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta tatcggtgct
    34501 tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga tgatgaaacg
    34561 ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc acctagcttt
    34621 gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc ggtcattttc
    34681 tatcagacta gcccagacat ggaaaacgga tttcctggaa ctttagatgt agaagtacgg
    34741 tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag tgaccgcaaa
    34801 acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc agcacagtca
    34861 atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa taaagataca
    34921 acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca acgtcctaaa
    34981 aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg cggtatcgat
    35041 catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt gacaagccca
    35101 gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat ttttacagca
    35161 aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca tggcggtatc
    35221 acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg agatgtcact
    35281 ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat aaagaaatag
    35341 aataaattaa aaagggagtg tgacagtgtc acatcccctt ttta
//
LOCUS       DAGCWU010000012        34443 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.012, whole genome shotgun sequence.
ACCESSION   DAGCWU010000012 DAGCWU010000000
VERSION     DAGCWU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.012"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..880)
                     /locus_tag="MBZ71_000467"
     CDS             complement(<1..880)
                     /locus_tag="MBZ71_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBT4428445.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKFRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTT"
     gene            1100..2293
                     /locus_tag="MBZ71_000468"
     CDS             1100..2293
                     /locus_tag="MBZ71_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4428446.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYVTRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAGQLVQLGWQYTFLIYA
                     ISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIVVYNAVSI
                     KLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSLLLMGIGF
                     VLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKHAESFNTSILLVGANLGT
                     FIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIRVDEEIKE
                     QLTTK"
     gene            2315..4330
                     /locus_tag="MBZ71_000469"
     CDS             2315..4330
                     /locus_tag="MBZ71_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBT4428447.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TETGGGILFDFGRAVNGCVELISLDDHIESITLCYRESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4413..5786)
                     /locus_tag="MBZ71_000470"
     CDS             complement(4413..5786)
                     /locus_tag="MBZ71_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBT4428448.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(5812..6096)
                     /locus_tag="MBZ71_000471"
     CDS             complement(5812..6096)
                     /locus_tag="MBZ71_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4428449.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6098..8113)
                     /locus_tag="MBZ71_000472"
     CDS             complement(6098..8113)
                     /locus_tag="MBZ71_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4428450.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     THIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFDSFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDDPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8292..8597)
                     /locus_tag="MBZ71_000473"
     CDS             complement(8292..8597)
                     /locus_tag="MBZ71_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBT4428451.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHY"
     gene            complement(8686..9717)
                     /locus_tag="MBZ71_000474"
     CDS             complement(8686..9717)
                     /locus_tag="MBZ71_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4428452.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNILVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIKSGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTTNSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            9924..10112
                     /locus_tag="MBZ71_000475"
     CDS             9924..10112
                     /locus_tag="MBZ71_000475"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4428453.1"
                     /translation="MLIEEKLTKQELFTTTEKRIADYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKNGIQRI"
     gene            10093..10800
                     /locus_tag="MBZ71_000476"
     CDS             10093..10800
                     /locus_tag="MBZ71_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4428454.1"
                     /translation="MGFSGFKEFRFELGWEVHQLIAQFNQTIDPNFPFSSEDSTQQIA
                     KKMADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKL
                     NKFLIMAEEYSDSSWNAANLISEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGN
                     QHSEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLK
                     NKQELMQKGLLKEYRKT"
     gene            complement(10952..11545)
                     /locus_tag="MBZ71_000477"
     CDS             complement(10952..11545)
                     /locus_tag="MBZ71_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4428455.1"
                     /translation="MHNKIHNKERLLIWAFFLKQIQLTIILDHNFFDLLFFDWKWQRA
                     SSVLLEGTSYPTGGENTHAVISSHRGLPQAKLFTDLPKLTLGDSFYIEINGRTLAYQV
                     DQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMVNSHRLLVRGHRVPYHAKEVQKEVQK
                     VSENKRFFLYGLLAACILIFLLLIYLFRQSFKIRNRS"
     gene            11549..12277
                     /locus_tag="MBZ71_000478"
     CDS             11549..12277
                     /locus_tag="MBZ71_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HBT4428456.1"
                     /translation="MRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLGKLKYSSYI
                     KVVNYFVNTIFSEFSEKEKNEISEQIRKNMSYCSFSSSDCKEKNEEYVVQLLTQIYFF
                     SRIFPYDTTHLTICATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFW
                     MLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSK
                     YNLLEYDLNVSFANEEELIRLFEL"
     gene            complement(12350..12997)
                     /locus_tag="MBZ71_000479"
     CDS             complement(12350..12997)
                     /locus_tag="MBZ71_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBT4428457.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKIGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNKKWLELLRI
                     NHFSIQSSEEAVSFLYFSMSLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(13005..13691)
                     /locus_tag="MBZ71_000480"
     CDS             complement(13005..13691)
                     /locus_tag="MBZ71_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428458.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(13697..14770)
                     /locus_tag="MBZ71_000481"
     CDS             complement(13697..14770)
                     /locus_tag="MBZ71_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428459.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEEGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHELKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            14993..15667
                     /locus_tag="MBZ71_000482"
     CDS             14993..15667
                     /locus_tag="MBZ71_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4428460.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIETLRNVG
                     YILQRV"
     gene            15672..17012
                     /locus_tag="MBZ71_000483"
     CDS             15672..17012
                     /locus_tag="MBZ71_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4428461.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMATIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            17101..18527
                     /locus_tag="MBZ71_000484"
     CDS             join(17101..17674,17674..18527)
                     /locus_tag="MBZ71_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4428462.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKATFMYWQKRLDRK
                     NPDQGIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSLVQFRKTTVTVV"
     gene            18716..19384
                     /locus_tag="MBZ71_000485"
     CDS             18716..19384
                     /locus_tag="MBZ71_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBT4428463.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(19584..19970)
                     /locus_tag="MBZ71_000486"
     CDS             complement(19584..19970)
                     /locus_tag="MBZ71_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428464.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(19942..21324)
                     /locus_tag="MBZ71_000487"
     CDS             complement(19942..21324)
                     /locus_tag="MBZ71_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBT4428465.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(21480..22667)
                     /locus_tag="MBZ71_000488"
     CDS             complement(21480..22667)
                     /locus_tag="MBZ71_000488"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBT4428466.1"
                     /translation="MEIKRSLNTKFAEIFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(22674..23705)
                     /locus_tag="MBZ71_000489"
     CDS             complement(22674..23705)
                     /locus_tag="MBZ71_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBT4428467.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(23751..25175)
                     /locus_tag="MBZ71_000490"
     CDS             complement(23751..25175)
                     /locus_tag="MBZ71_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4428468.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            25350..26255
                     /locus_tag="MBZ71_000491"
     CDS             25350..26255
                     /locus_tag="MBZ71_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBT4428469.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            26346..27023
                     /locus_tag="MBZ71_000492"
     CDS             26346..27023
                     /locus_tag="MBZ71_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBT4428470.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            27020..27190
                     /locus_tag="MBZ71_000493"
     CDS             27020..27190
                     /locus_tag="MBZ71_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428471.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            27304..28200
                     /locus_tag="MBZ71_000494"
     CDS             27304..28200
                     /locus_tag="MBZ71_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4428472.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(28255..29625)
                     /locus_tag="MBZ71_000495"
     CDS             complement(28255..29625)
                     /locus_tag="MBZ71_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4428473.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            29863..30609
                     /locus_tag="MBZ71_000496"
     CDS             29863..30609
                     /locus_tag="MBZ71_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBT4428474.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            30606..31445
                     /locus_tag="MBZ71_000497"
     CDS             30606..31445
                     /locus_tag="MBZ71_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBT4428475.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            31446..32396
                     /locus_tag="MBZ71_000498"
     CDS             31446..32396
                     /locus_tag="MBZ71_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBT4428476.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            32537..33289
                     /locus_tag="MBZ71_000499"
     CDS             32537..33289
                     /locus_tag="MBZ71_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428477.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            33308..>34443
                     /gene="queG"
                     /locus_tag="MBZ71_000500"
     CDS             33308..>34443
                     /gene="queG"
                     /locus_tag="MBZ71_000500"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBT4428478.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLR"
BASE COUNT        10994 a         6423 c         6622 g        10404 t
ORIGIN      
        1 aagtcgtttg tttatatccg atacgattag cgatttcttc caacgaaaaa ctcgtttctt
       61 gaatcagttc caacgcaaag tcgaattttt tctttagaac cagttcttga aaggaagcat
      121 caaaatacat tttcaattta ttactaagat agaccggatt gtagccaaaa tgagctgctg
      181 tttcttttaa cgtacaagaa gtaaaatgct tatcgatata atctaatact ctaagcatct
      241 cattttctgc gcatccatct gctgatgaaa agctttgttg ttctaataaa tgagtcaatt
      301 cgatcagtaa tagtgatgta tagagattta acgaacggaa cttatgactg tatgtatcaa
      361 aataaatcaa cagcatattt tcaatcaaat gcgtcaaacg acttgttttt ccgccgcgaa
      421 aatgaatgaa ttgtgtatgt tccccttttt cttttgatgc attgatcaaa aaagaagaaa
      481 gtagattatt tgtgcttgcc aaatgattca atatctctgt agataaggtt tcttctttta
      541 ataagatgtt gattaaaata tcctcttgcc ctgtgtattc gattctttga gtagcttctt
      601 tatcaatgat caccacgtca ctttcttcca tctctatcct ttctccattg ataatttgta
      661 aacatgtacc gctatacata taattgattt caacaaaatc atgtgtatgc tcgggaactt
      721 ccgcatatcg acggtgttta ctgatatgaa tatttccctg ttgaaataga tctcctcggt
      781 ggatttttgg cggtgtctga agatggtaag ctaagtttaa atcattgata ttcattcctt
      841 gttcacgctg cagagtttcg attgattctt cgttacgcaa aaaagtgtct aattcagcta
      901 gttccaattg actccctcct ttttgtaagt ggtttctttc gcttattagt ttacgctgta
      961 ctgctattat atgcaaagct atttttggtt tcataaagac tttctatggt tgggacatga
     1021 acttcctgtg attccgctat tgagagaata accacttcat actatatttt tgataagcgc
     1081 ttacaaaaat gaaaggaaca tgcttatgaa taaaaagatg atgaaatttt ctctgctgtc
     1141 agtctctctt attctgactt ctgctggtgc agtcacgtct gcaattccca gtatgtcagc
     1201 tacttatgtc acgcgttctt tatctgctgt tgaattatta acgactgctc cctcctttat
     1261 ggtcactatt tttgttttga ttagtagttt tattgctagg aaaatcggac aaaaacaaac
     1321 tgtgatcatc ggattagtga ttgcatcgat ttgtggtatc ctccccctct tctcacaaag
     1381 ttacagtatt gtcttagctt caagagctgg actaggtatt ggctttggat tgatcaattc
     1441 tttggcagtc agcatgattg ctgcctttta tcacggagat gaacgagcct ctatgattgg
     1501 ttttcaaagt gcatttcaag gatttggtgc aggacagcta gtacagttag gttggcaata
     1561 taccttttta atctacgcca tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc
     1621 tacacctgaa acaactgaac ttcaatcaca tggcacagcc ccaacaagac aagttatcaa
     1681 aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg tggtatataa
     1741 tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa acgaaactaa
     1801 tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag tttgttttgg
     1861 ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac tgatgggaat
     1921 cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct
     1981 aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa gtatccaagc
     2041 gcctaagcac gcggagagtt tcaatacttc aatcttattg gttggcgcta atttaggtac
     2101 tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga gtaaaacact
     2161 tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta gccttctagt
     2221 agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa aagaacaatt
     2281 gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag
     2341 atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa atttccaacg
     2401 ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc atcggaacgt
     2461 caagtttaag agatattact ttcttgatca accttccatg tttaaagtga cgatccaagg
     2521 agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc tgacttgtcc
     2581 agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac cagcgatgtt
     2641 tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt ttattgaaga
     2701 atatccagct ggctggagtc cgctttatgt cgatatcgca aaagacccaa atcaaatcta
     2761 ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag actggcggag gaatcttatt
     2821 cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg accacatcga
     2881 atctatcact ctatgttata gagagtccga tgtagaagct atggatatcg attattgcta
     2941 ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt
     3001 gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg aatttttatc
     3061 tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga tctggtcagt
     3121 atcagaagaa accttcaagt tatgtagtga tttgtttttt atcgacggta tcaaacgaga
     3181 tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat atcttttttt
     3241 tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg aaatcaagca
     3301 gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg aaaaccacta
     3361 tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc aatcattaat
     3421 gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag caaaagattg
     3481 gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag aacaagtctt
     3541 attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg aaacatatgg
     3601 agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa
     3661 cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct atgaatctgg
     3721 gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa
     3781 agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca cacagattac
     3841 tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac agacgaatat
     3901 cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg cggtaacttt
     3961 ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt atggagatcc
     4021 ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt taggacgcta
     4081 ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac aacctaatct
     4141 agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga tttcgcttga
     4201 aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag tgaaaatgaa
     4261 tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct attcaaaaat
     4321 aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc ttcttctgat
     4381 attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga aacaatgttt
     4441 gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa agcgatgatt
     4501 ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag tcctggtact
     4561 agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa tactggaagg
     4621 ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa taccgcccct
     4681 cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct
     4741 actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat ggatagtgtt
     4801 cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata tggaaaaacg
     4861 ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat cccttggaat
     4921 gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac aaacccagct
     4981 gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt gcttagttct
     5041 ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat cgacatcaag
     5101 agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc
     5161 tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga agcataaccg
     5221 atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt cataaacggt
     5281 tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc taataagatc
     5341 atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag acaagaaaca
     5401 taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaaccg agcaagtaaa
     5461 atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa
     5521 gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag agctgtttgg
     5581 aacatcgaag caaaaggggt tagtgaatta ctgattagac cagcaccgcc gatcagtact
     5641 aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt cttcttctga
     5701 agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact gacgattgac
     5761 acaaacaaat tcaacaattt atccattgat agttcctccc ttgaattatt tttattcttt
     5821 tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg attgcaaagg
     5881 tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct caatgaagaa
     5941 ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt caaactcgtc
     6001 cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca taaaacttgt
     6061 tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct tttcttatga
     6121 tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt tgtttattag
     6181 aaagtagctt tgttaaattt gccaacgccc gaagatgcgc ctcattatcg atagcagcta
     6241 aacagataaa caaactaatg ggatgggcag gatcatcaag cagatccaca ggttgttgaa
     6301 gtttcagcaa agacatccct acttcattta cgccatcttc tggacgagca tgaggtaatg
     6361 caatgtggtc gccaatatgg ataaacgcac catactgctc cacgcgctcg atcatagctt
     6421 tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca aatgtgattg
     6481 cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt aataattcag
     6541 atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta cgatttaata
     6601 cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc agtagcgccg
     6661 acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct ctttgtactt
     6721 gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca tagagagact
     6781 ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt
     6841 caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc gggaataatt
     6901 gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag acgatcacgg
     6961 ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg ccaaatagta
     7021 tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt tctaacggaa
     7081 gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt tgatcgatca
     7141 tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc aagtgataaa
     7201 agacattcat caataattca cgaaacgaat caaattctac ggccatcagg aattccatgc
     7261 gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc tggacatctc
     7321 cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt tgatgttcgg
     7381 aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga gaagccaaca
     7441 gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt gtcaaactca
     7501 aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat agtgaaatcg
     7561 ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt gcggttaaat
     7621 acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga ttttttgctt
     7681 ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta gtatacgaca
     7741 gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac aggactgtcc
     7801 ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat
     7861 aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct tcaaatcgag
     7921 taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata cggatatggg
     7981 tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt gtatttactt
     8041 cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg agataactaa
     8101 ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta gcgcttttaa
     8161 atcgaggggt gtttggtaca acttacagtt ggattttttt tacgaatgta ccgaacgact
     8221 tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg
     8281 atttcgtccg atcaataatg atcatgttta ttttttgagg tcagttcttc aaatttttct
     8341 ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc attaggcttt
     8401 gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc tgtttcacca
     8461 gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt agttcctgct
     8521 aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt gtgtttttca
     8581 ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca tcgtacaata
     8641 agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata tttttccttc
     8701 tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga ttgtttcttc
     8761 atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt cgtagacacg
     8821 gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt gttcatctat
     8881 ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat gatcaggcat
     8941 gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga acatcccttt
     9001 ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg attttatcga
     9061 aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc gtcctttccc
     9121 taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag aaagaacttg
     9181 atctactgta tgacaagcat gtccatataa atagctgttc gtagtagaaa attcatgcgt
     9241 attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta aaatatcccc
     9301 taattttccg cttttgatta ctttttggac tgtcaagaag tcagaatcga accgtctgtt
     9361 ttggtaacat tggataaata gccctttttc ttttgctaat gcaaataact cttctgcttc
     9421 ctttgacgtt tctgcaaaag gtttctctac tagtatattc ttaccatgtt cgagtgccat
     9481 ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt ggatctcagg
     9541 atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg ttttccacac
     9601 tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt ttcttgaaaa
     9661 aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg taagcatgag
     9721 tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa agtatttata
     9781 atagctattc tggtaaaaaa gttcagaaaa acacttgtta ttattcggtt tgatgaaatt
     9841 aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta
     9901 agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa caagagttgt
     9961 ttaccacgac agagaaacgg attgccgatt acatccgtag gaatatcgaa gctgcagtct
    10021 acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct atcattcgtt
    10081 tatgcaaaaa aaatgggatt cagcggattt aaagaattcc gtttcgagtt agggtgggaa
    10141 gttcatcaat tgattgctca gttcaatcaa acgattgatc ctaattttcc tttttcaagt
    10201 gaagatagca ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca gtcaataaaa
    10261 aaagcacaat tgcagattga acatgaagat ttagatcaaa tcggtaaagt attgaacaag
    10321 gcacagcgaa tctttctttt tgcaaaaggt gattctcaaa tcacggcaag gaaattccag
    10381 aataaaatgg tcaagttgaa taagtttttg atcatggccg aagaatacag tgattcttct
    10441 tggaatgcag ccaatttgat tagcgaggat tgcgccattt ttattagcta cagcggtcgc
    10501 atccatcatt atgaacgaat aatgacctat ttgaagcatg tagaggcccc taccttgctg
    10561 attaccggaa atcagcattc agaaatggcc aaacaggctg atatgtgtct tgttatatcg
    10621 caggaggaat atgattttgc taaagtagca actttctcct ctcaaattgc cttcgattac
    10681 gtattaaata cgttattctc agtgatatat tctcaaaatt ttgaagagaa tatccgcaac
    10741 ttgaaaaaca aacaagaatt gatgcaaaaa ggccttctta aagaatatcg gaaaacttaa
    10801 gaaaagatcc tcatttagta atgaggaaga tagtgtttga taggctctct ttgatatcat
    10861 cataaaaaca ggctgaagtt ttctttttca aacttcagcc tgtttgttta cggaacgatt
    10921 cgaatagata tattatagaa atgaggaata gctaacttct gtttcttatt ttgaacgatt
    10981 gacgaaaaag atatataaga aggaggaaaa tcagtataca tgcagctaac aatccataca
    11041 ggaaaaatcg tttgttctcg gatactttct gtacttcttt ttgaacttct tttgcatgat
    11101 aaggaacccg gtgccctctg acaagtaagc gatgactgtt gaccatataa ggtgtacaag
    11161 ttaggagtgt cacgaaatcc tgcccttttt cgattctcag atcttttgta tcagtcggtt
    11221 ctaccgtctt gatctgatcg acttgataag cgagtgtccg tccattgatt tcgatataga
    11281 aagaatcacc taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga ggcagtcctc
    11341 gatggctgga aataacggca tgtgtatttt ctccccccgt aggataagac gttccttcca
    11401 ataatacgga agacgcacgc tgccatttcc aatcgaaaaa aagaagatca aagaaattat
    11461 ggtcaagaat aattgtcagt tgaatttgtt tcaggaagaa agcccatatg agcaatcttt
    11521 ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg
    11581 gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt
    11641 gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt
    11701 caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg
    11761 aaaaaatatg tcctactgtt cgttttcatc ctctgattgc aaagaaaaaa acgaagaata
    11821 tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac
    11881 tcatttgacg atctgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct
    11941 atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat
    12001 ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta
    12061 tacttctgat aaaaaaatac tgatcatcag tgacttaagt cttcgacata gtcaatatat
    12121 tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat
    12181 tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgaatg tgtcattcgc
    12241 aaatgaagaa gaactcatcc gcttattcga gttatgaata atcaaaaaac agctatgtat
    12301 aaaccaccct gataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc
    12361 cttcagcaat ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat
    12421 tatttgcaat gagatccgtc aatgtgttca ttacgaggga catggaaaag tataggaaac
    12481 taactgcttc ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt
    12541 ttttgttcat tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc
    12601 gctgggttaa caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc
    12661 actctaaata tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta
    12721 agatatcttg atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca
    12781 aatcttgaaa atatttataa aaagcaccgc gcgacatccc tatcttgtca acgatctctg
    12841 agactttgac ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc
    12901 tctgtttctt tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt
    12961 tatctctgat tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc
    13021 tcttcttctg tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata
    13081 cggacatcat gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt
    13141 aatttgacga cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag
    13201 atcaagcttg gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca
    13261 gaaaggtctt taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa
    13321 tcgatttttg ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc
    13381 tttaccgtta aaaaaggaat caaattagag ctttggagaa taaatccaat ttctttaaag
    13441 cgtaaatctg ctcgtttttt ttctggaagc tcagttagag agtgaccgtt gatcactatt
    13501 tctccggaag tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa
    13561 ccagaaggac caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc
    13621 aaagctgaga cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat
    13681 atttttttca tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac
    13741 gtatcgaaaa caatccgcct agcattgcaa caatgatcaa aatcccacta tatagcaacc
    13801 attgtgacca aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag
    13861 caaacaaaag agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag
    13921 atttagcgat gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct
    13981 tctgaagtgt catcacgtac atgaaaatac cgaccacagc agcaaccact aagaacaaaa
    14041 agtatatcat cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga
    14101 atgtcccaat agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg
    14161 aatcctttat tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc
    14221 catacttcaa agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat
    14281 aagtttcagg aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg
    14341 tatctccaat tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg
    14401 ttttaagttc atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga
    14461 aaactgtaac attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg
    14521 ctttctctcc ttcttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata
    14581 cttgatttgc atcctccgat aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt
    14641 taaactcttc tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca
    14701 agaacataat accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag
    14761 ctagaaacat ttgtttttcc tccctatgat agattgatag tgacaaaatg tcactcgttg
    14821 aatagtgtaa ctccaaagtg acattttgtc acttatgttg ttttttttct tatcaataaa
    14881 ttgtctaaga catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct
    14941 tatttatgct ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa
    15001 actattagta gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc
    15061 aatcggcgag ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg
    15121 agtttatgat cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt
    15181 agcaaaaata cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag
    15241 tttggaagat aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt
    15301 ctatcgagaa gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt
    15361 tgatgaaaat gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt
    15421 gtataaagaa caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt
    15481 acaaaataag ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga
    15541 ttctgatacg acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg
    15601 aagtactgga ttggataaag aaatcgaaac cttacgtaat gtcggttata tactgcaaag
    15661 ggtgtgatgc aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa
    15721 atgtagcagc atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa
    15781 accaatctgc atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa
    15841 cagtacaatt agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa
    15901 cggaacaaaa cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta
    15961 acactcaagt gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg
    16021 ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa
    16081 atattgaatt aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa
    16141 aagcggagac gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa
    16201 tcaagaattc attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga
    16261 ttctctctat tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt
    16321 cttggagaag acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa
    16381 cgattattca aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag
    16441 aatccgaaag catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg
    16501 atttgctgac aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca
    16561 atacacaaga atatatcgaa caacttgtta aaccatttca agagatggcc accatagatg
    16621 gaaaagagtt tattctccat aacgatgcaa cattagaagt accaattgac gaaaaaaaaa
    16681 tccatcaagt tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata
    16741 agatcatttt ggactcatct gttaaccata aagaatggct gattcacgta aagaatactg
    16801 gcccttctat cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc
    16861 aatctagggc gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa
    16921 tcgaacagca tcatggacaa atttctgtat ccaattatct tccacaagga gtcgatttta
    16981 aagtccaatt gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca
    17041 aatgtaccga cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtatttttt
    17101 atggctaagt atacttttga acttaaatta aagatcgttc atgattactt ggatggtaaa
    17161 ggcggaagtg attatctggc aaagaaatat tccattaagg ctccatcaca agtgaagcgc
    17221 tggatcaatg cctatcaaga atttggtgaa gagggtctag taagaaagcg acaaaagaaa
    17281 aaatattctg ttcaatttaa gctggatgcg atagagttat atctaacaag tgagctgtcc
    17341 tatcgtgaag ttgccaacac acttaacatg aacaacccta atttgatcgc tagttggatg
    17401 cgtcaattcc gtgagggtgg gatcgatgga ctctcaaaaa cgaaaggatg tccacctatc
    17461 ttgtctaaga aaaatgaacc caaaaataag aaaaaatcta caactaaagc aacgtctaaa
    17521 gaacgtgaac gcatcgagga gttggagaaa cgagtccgtt cccttcaaat tgaaaatgcc
    17581 tttttaaaag aattgaggaa gctgcgaaaa caggaagccc aacaacgacg aatgaagcaa
    17641 tcgcacgaat catcgcaagc ctccgaggac cgtttaaatt agttgagctt ctagaaactt
    17701 taaagttccc taaggcaacc tttatgtact ggcaaaaacg attggaccgt aaaaatccag
    17761 atcaagggat tgaagcggaa atgcttaaag ttcgggagaa gcacaaggat tatggctgtt
    17821 tacggatgac caatgaacta agaaaccgcg gtttctctat caataaaaag aaagttcagc
    17881 gcctgatcaa gaagctagga ataaaagtaa cgacatacac acgtaaatca cggcgctaca
    17941 attcttatcg aggacaagtc ggagcagtag ccaaaaatcg gatccatcgt cgtttctaca
    18001 ccagtatctg ccatcagaaa atcacgacgg atacaactga atttaaatac catgaagtgg
    18061 atgccaaagg cattatccgt caaaagaaat tgtatctaga tccttttatg gatatgttca
    18121 atagtgagat tttatcctat cgtatctctg aaaagcccaa tgcattagcg gtaatggaag
    18181 gtttggagga agctatccag accactaacg attgtcccta tcgtcgtacg tttcactcag
    18241 atcaaggttg ggcgtatcaa atgaaagctt atagaaacaa attgaaggag tacaaaatct
    18301 ttcaaagcat gtctcggaaa gggaactgct tagataattc acttatggag aatttttttg
    18361 gcttattaaa acaagagatt tttcacggga aagtttataa ctgtttcgag gaattaaaat
    18421 cagccatcga tagctatatc tattactaca ataatgaacg gataaaacag aaattaaact
    18481 ggcaaagtct cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta
    18541 gagcataaaa aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac
    18601 tacagtgtca cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc
    18661 gtgttcgtga gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat
    18721 tttttcaatt acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt
    18781 gcttgggaag aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt
    18841 aggtgggatc ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt
    18901 tgccattgcg ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga
    18961 aaaggtcaat cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa
    19021 catgacagag ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca
    19081 gcaagaatgc ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat
    19141 gagtgtattg aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg
    19201 gcaaattcag cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa
    19261 cttgaagaaa gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga
    19321 aaaagggctc tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg
    19381 atagatagac agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt
    19441 tttcacaacc tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag
    19501 ccaaaattcc acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag
    19561 ttgatcactt ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca
    19621 atataccgta cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag
    19681 gcttcaccaa tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa
    19741 ttgagctgaa tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt
    19801 tcttgcaatg tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt
    19861 ttgataccgt tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc
    19921 cgatctcgat actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc
    19981 ttctaatcca ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg
    20041 tcctccttgc aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat
    20101 cgtatcgaca acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag
    20161 gttaagctgt aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat
    20221 atcctggtag tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct
    20281 agcatttgtc gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag
    20341 atagcctaaa gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct
    20401 caagcgagtg ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct
    20461 tgtataatct ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc
    20521 ttgtcccaaa aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac
    20581 ccctagcttg cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat
    20641 acccatttgt ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc
    20701 tgagattttc cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt
    20761 tccgtctttt gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc
    20821 tatagtcgtg tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc
    20881 gtacgcattt gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc
    20941 gacggaatag acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc
    21001 tctgttcttt tccatataat aggccatcct tggggcaaca atatataaat cacttggata
    21061 ggggaaggga agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc
    21121 aggactggaa gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga
    21181 catgactact aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc
    21241 gtaaaaagat ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa
    21301 taccggattt tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt
    21361 ctctttccgt gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg
    21421 aaattgctga gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat
    21481 caaacatgaa cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa
    21541 aattccggag catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca
    21601 ggtatatttt cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt
    21661 cgtgcaccta agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc
    21721 ccagttactt cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat
    21781 gcttctaagt tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa
    21841 acggcatggc gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca
    21901 aattcttctt catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc
    21961 aaggcttctt cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc
    22021 ttattcgtat tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat
    22081 ttcagcgtat ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac
    22141 tgatccatga tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg
    22201 actagttcca agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat
    22261 aattctaatg aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat
    22321 aactcaaacc catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt
    22381 acgtaatttg cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg
    22441 acaccgatat cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct
    22501 gctacacctt gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct
    22561 gtgtgttcac caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt
    22621 ccgaatattt cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc
    22681 tttcttgttt tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga
    22741 atcgaaccaa atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg
    22801 ccaccgtgat gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca
    22861 gtgaaaataa ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg
    22921 ctagtcaagc gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg
    22981 attccgtcaa caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta
    23041 ggtatttgaa aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta
    23101 tctgttcgca aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac
    23161 tgagaagcat ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct
    23221 tggtcactgg tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg
    23281 atttccacat ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa
    23341 atgacagaag cttcattttc gccattcaaa atgacataag accatttttt taattcaaat
    23401 ccaggagcac caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt
    23461 tttccattga gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca
    23521 ccgatatacg gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct
    23581 cgatcatttg attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc
    23641 ttgtttttgt tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc
    23701 ttcattttca agatcctttc ttaatgggaa ggaactactg catcgtgaag ttaatcttcc
    23761 attgtatcag ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc
    23821 tctgtcaatg taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga
    23881 gcatagaatg cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta
    23941 gcattaccag tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca
    24001 ccagcgattt tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca
    24061 tttttccatt gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc
    24121 aattgttgag ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct
    24181 gcgatcgtaa agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag
    24241 cgacgagaga tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag
    24301 gcagcaacta gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag
    24361 tagaataata cggcagttgt tgctgcattg gcaatcgcat aagcgatata agcaagggca
    24421 agccacatca actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt
    24481 tcattttgct gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca
    24541 gtaccgcctg aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca
    24601 atatgtttat gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta
    24661 gtaccatttg cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc
    24721 tttgtttccg tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa
    24781 aaactatcca ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga
    24841 gtagctaagc caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg
    24901 ccgataacta accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca
    24961 ccaatcaaag gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact
    25021 gtagcaatca tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg
    25081 aataccataa aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa
    25141 gcgtaggaca cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat
    25201 atataatact aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt
    25261 aacaagaaat cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca
    25321 ttatactaaa ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac
    25381 atcaacaagt agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt
    25441 ttcattcttt tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca
    25501 aaggatctta tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga
    25561 ttcgtccagg agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct
    25621 ggctttcatt tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag
    25681 accaatacac gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca
    25741 tgaaagatac ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt
    25801 ttttatcagt ccttttaaaa gatggcggaa aggtcagcgg aggtgtacaa aaggttcatt
    25861 cctctttagc tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg
    25921 ttgaggaaat cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa
    25981 atcatatggg caccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt
    26041 ttcttctttc tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata
    26101 gtctagtcgt atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc
    26161 gtaaacgtat gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt
    26221 tggaaaaaca aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat
    26281 cggcaaaaga aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa
    26341 aagggatgcg tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc
    26401 aatttttcaa agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt
    26461 atccagacgg aaaaatagat atagagactg aactacagct cttggcccag catgatcgga
    26521 tcattttcca gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag
    26581 ataatgtctt ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga
    26641 caacaggtgt ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg
    26701 aatttttgag gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat
    26761 tcgttttagc acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt
    26821 atcagcagta tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta
    26881 ttcaaagagt acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata
    26941 tcgtagaagc actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag
    27001 aaatgaaagg aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag
    27061 cgaaaaatca gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga
    27121 gatagacgaa cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct
    27181 caactggtga aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt
    27241 gctacaataa gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa
    27301 aaaatgtatg atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa
    27361 aaacaggcag ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc
    27421 atagcaggag gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt
    27481 acgaccataa cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca
    27541 actaaaacaa aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa
    27601 cttcaagcaa tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt
    27661 gaagaatacc agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt
    27721 gctaaccaaa aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt
    27781 gcagcgtatc aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa
    27841 ttatcggcta attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc
    27901 gcctctcttc aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca
    27961 gcagagtcgg tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc
    28021 tctgaaacta ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga
    28081 gaaggtccaa atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg
    28141 aatgggatga gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga
    28201 taaaagcaat aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt
    28261 tccggttctt ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc
    28321 aataatcaat gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa
    28381 aaaagcaagt gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg
    28441 cattttatct gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg
    28501 aaccatacca ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt
    28561 gatgaacatt ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc
    28621 tacgacagga taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt
    28681 tccatttatg gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc
    28741 tgataatgcc gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac
    28801 tttagaaaaa gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat
    28861 actcacaatg ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg
    28921 gacagcttca atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag
    28981 ttcgattcct tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc
    29041 tccagtgaag aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt
    29101 accaaatcca aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac
    29161 tttgatgatc gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc
    29221 cagcataaga aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca
    29281 ataattcata taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa
    29341 aatattactc caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt
    29401 tgcaaaagaa ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa
    29461 atacaaaaag aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat
    29521 ggttgaggag gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg
    29581 gatatgacgg gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc
    29641 gttgttgagt caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg
    29701 ttagccttat tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa
    29761 ttcgaaaaaa actttttcat cattctactt gaaaatgctt tccattttag ttaaactata
    29821 tttaaataaa aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt
    29881 gtaattcaag acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg
    29941 acgataaatc cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca
    30001 tttatgaaat ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga
    30061 cagccggtaa actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat
    30121 ctttcctttc caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga
    30181 attggacaaa gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta
    30241 gggatggaag aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga
    30301 gtttttattg caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc
    30361 gtagggattg atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat
    30421 tcaggaaaaa cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat
    30481 gaggttattc ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca
    30541 tcgaagaacc ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag
    30601 aaaaaatgat ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt
    30661 tggtcacttc catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac
    30721 gagggatgtc tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt
    30781 cttatatgtt tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag
    30841 cagtgattgg tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg
    30901 tctttagctc cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag
    30961 atttgtatca tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga
    31021 cagcactcgt tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga
    31081 tcacatcatt cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt
    31141 atcttttgat gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa
    31201 tcttggtgat tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt
    31261 tactgaaaat gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt
    31321 ttttcagtta tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat
    31381 tttttatcct tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca
    31441 gatagatgaa aacaaaaaaa tcactctttt taatcttagc agttagtttc ttggtattag
    31501 ccggttgcgg aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag
    31561 tcgtggctac caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag
    31621 atatccacag tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag
    31681 acatcaaaaa ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag
    31741 gtaacagctg gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt
    31801 ttgcagttag caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag
    31861 cagatcccca tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac
    31921 gtatattctc tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat
    31981 atgtggaaaa actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag
    32041 agaacaaaaa attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg
    32101 atttgccagc agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa
    32161 tgaaagcgat cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca
    32221 gtgtagactc aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata
    32281 aactgttcac tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga
    32341 tgaaatggaa tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag
    32401 tgcaaggtct agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt
    32461 atgctgtttt tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag
    32521 gaaggaaggt gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt
    32581 ctttcgattg atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga
    32641 agcagaatta gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga
    32701 gctgtacata ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat
    32761 cggttatttt gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa
    32821 cgacgaaata accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt
    32881 aatcaaaaag atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat
    32941 gaatatgttt tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt
    33001 cacgtacaca cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga
    33061 gttttcgcca tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt
    33121 gaattttttt aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt
    33181 atttgctgca agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt
    33241 tgtccagctg aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag
    33301 gaagaccatg acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa
    33361 aatcgggttc accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa
    33421 agcagctggc catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga
    33481 aatgacattt gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat
    33541 ccatgaagaa gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat
    33601 cgattaccat gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag
    33661 agcagaaaaa tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga
    33721 actgatcgat gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt
    33781 gttgatcact gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt
    33841 atttgagcct gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc
    33901 ttgtccgaca aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta
    33961 tcaaactcag acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat
    34021 ttatggatgt gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt
    34081 tcatgaagag atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc
    34141 gatgtctaat aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa
    34201 gaaacctttg cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt
    34261 gcctgagatt tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg
    34321 ggctattgga cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat
    34381 attagctaaa gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt
    34441 gcg
//
LOCUS       DAGCWU010000013        32930 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.013, whole genome shotgun sequence.
ACCESSION   DAGCWU010000013 DAGCWU010000000
VERSION     DAGCWU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.013"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            44..1185
                     /locus_tag="MBZ71_000501"
                     /pseudo
     CDS             44..1185
                     /locus_tag="MBZ71_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1311..2390)
                     /locus_tag="MBZ71_000502"
     CDS             complement(1311..2390)
                     /locus_tag="MBZ71_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4428479.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2493..2933)
                     /locus_tag="MBZ71_000503"
     CDS             complement(2493..2933)
                     /locus_tag="MBZ71_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4428480.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2990..3400)
                     /locus_tag="MBZ71_000504"
     CDS             complement(2990..3400)
                     /locus_tag="MBZ71_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBT4428481.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3580..4014)
                     /locus_tag="MBZ71_000505"
     CDS             complement(3580..4014)
                     /locus_tag="MBZ71_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4428482.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4114..4962)
                     /locus_tag="MBZ71_000506"
     CDS             complement(4114..4962)
                     /locus_tag="MBZ71_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4428483.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5351..7117
                     /locus_tag="MBZ71_000507"
     CDS             5351..7117
                     /locus_tag="MBZ71_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBT4428484.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7219..8478
                     /locus_tag="MBZ71_000508"
     CDS             7219..8478
                     /locus_tag="MBZ71_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4428485.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8625..9926
                     /locus_tag="MBZ71_000509"
     CDS             8625..9926
                     /locus_tag="MBZ71_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4428486.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9929..10780
                     /locus_tag="MBZ71_000510"
     CDS             9929..10780
                     /locus_tag="MBZ71_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4428487.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10793..11608
                     /locus_tag="MBZ71_000511"
     CDS             10793..11608
                     /locus_tag="MBZ71_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBT4428488.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11759..12925
                     /locus_tag="MBZ71_000512"
     CDS             11759..12925
                     /locus_tag="MBZ71_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4428489.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13155..13802
                     /locus_tag="MBZ71_000513"
     CDS             13155..13802
                     /locus_tag="MBZ71_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBT4428490.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13886..14830
                     /locus_tag="MBZ71_000514"
     CDS             13886..14830
                     /locus_tag="MBZ71_000514"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBT4428491.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14965..16416
                     /locus_tag="MBZ71_000515"
     CDS             14965..16416
                     /locus_tag="MBZ71_000515"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBT4428492.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16437..17273
                     /locus_tag="MBZ71_000516"
     CDS             16437..17273
                     /locus_tag="MBZ71_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBT4428493.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17273..17932
                     /locus_tag="MBZ71_000517"
     CDS             17273..17932
                     /locus_tag="MBZ71_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4428494.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18058..18747
                     /locus_tag="MBZ71_000518"
     CDS             18058..18747
                     /locus_tag="MBZ71_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428495.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18753..19709
                     /locus_tag="MBZ71_000519"
     CDS             18753..19709
                     /locus_tag="MBZ71_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBT4428496.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19753..20265
                     /locus_tag="MBZ71_000520"
     CDS             19753..20265
                     /locus_tag="MBZ71_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4428497.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20521..21069)
                     /locus_tag="MBZ71_000521"
     CDS             complement(20521..21069)
                     /locus_tag="MBZ71_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4428498.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21158..22339)
                     /locus_tag="MBZ71_000522"
     CDS             complement(21158..22339)
                     /locus_tag="MBZ71_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBT4428499.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22575..24083)
                     /locus_tag="MBZ71_000523"
     CDS             complement(22575..24083)
                     /locus_tag="MBZ71_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBT4428500.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24242..24433
                     /locus_tag="MBZ71_000524"
     CDS             24242..24433
                     /locus_tag="MBZ71_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428501.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24636..25553
                     /locus_tag="MBZ71_000525"
     CDS             24636..25553
                     /locus_tag="MBZ71_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBT4428502.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25546..26619
                     /locus_tag="MBZ71_000526"
     CDS             25546..26619
                     /locus_tag="MBZ71_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428503.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26767..27285
                     /locus_tag="MBZ71_000527"
     CDS             26767..27285
                     /locus_tag="MBZ71_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428504.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27266..27658
                     /locus_tag="MBZ71_000528"
     CDS             27266..27658
                     /locus_tag="MBZ71_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBT4428505.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27734..28669
                     /locus_tag="MBZ71_000529"
     CDS             27734..28669
                     /locus_tag="MBZ71_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBT4428506.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28802..29581
                     /locus_tag="MBZ71_000530"
     CDS             28802..29581
                     /locus_tag="MBZ71_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBT4428507.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29574..29891
                     /locus_tag="MBZ71_000531"
     CDS             29574..29891
                     /locus_tag="MBZ71_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBT4428508.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30135..31745
                     /locus_tag="MBZ71_000532"
     CDS             30135..31745
                     /locus_tag="MBZ71_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4428509.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTIQSTM
                     LQSLNHHRFAIRLTVEAIILKVIFEVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31902..32750
                     /locus_tag="MBZ71_000533"
     CDS             31902..32750
                     /locus_tag="MBZ71_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4428510.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHVDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        10872 a         5900 c         6421 g         9737 t
ORIGIN      
        1 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
       61 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      121 agaggagaca gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
      181 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
      241 agcgaccatc aaagcaaaaa ttgactattc gggagactta gcacctatac tacaatcagc
      301 tgttacattt gagaatgttg atcccacttt gtcgattggc tatgacgaga acactttgac
      361 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
      421 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atttaacgcc
      481 gtatagtcag cggcagacaa ttgagcaaga ttcagccatc gactcaggag gagatcttgt
      541 tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa
      601 tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca
      661 accagagcca gacaataaat caaaacctga aataacccct ggagaaaaac cagatgtaac
      721 tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga
      781 taatgaaact gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac
      841 tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac
      901 aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa
      961 tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt
     1021 aaaaagtagc aaggatacaa cctttgctgt cctgaaacga aaaataaaca gctaccaaaa
     1081 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
     1141 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
     1201 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt
     1261 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
     1321 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
     1381 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
     1441 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
     1501 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
     1561 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
     1621 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
     1681 ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac aacaaattga
     1741 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
     1801 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
     1861 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
     1921 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
     1981 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
     2041 tcgtaaaaat taataacgga ctggatcatg tcaaatgttt gatgaataag catcgggata
     2101 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
     2161 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
     2221 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
     2281 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
     2341 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
     2401 aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg
     2461 gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc ttgatgaaaa
     2521 ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg ttcaagtgga
     2581 agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc agcaccttga
     2641 gcagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg attaatataa
     2701 tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag acacaaatca
     2761 tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat cacaagatac
     2821 tgggtatagg taagattcag cggcttgagg aggggagtgt agtgtctaat gatttcttta
     2881 gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc catttttcca
     2941 cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc tattcactaa
     3001 ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga cacactttat
     3061 gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata gatccttcaa
     3121 tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact tgtgcactga
     3181 cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca ctgttaaaac
     3241 atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg acgcgtttac
     3301 caggagctgc aatattcaat tcgaatgtac gatcgggatt atgcacttct ccagagcgtc
     3361 cgcctgtgtt gatcattttc gtagaataca tttttttcat aaagttacca cctttaatta
     3421 tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa tggattggca
     3481 taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc aaacatatac
     3541 aaaaaatatc agagacaaaa aataatctca tcataaacat tacatatatt tctcaaaaag
     3601 ttgttttctt tcttcaaaaa ttacttcacg atggacctga tgaagcatcc ataaataacc
     3661 aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg
     3721 aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat
     3781 gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt
     3841 gttttcatcc agcatatgga atctcgtacc atacaaggta aaaatgactt cattattgcc
     3901 tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc
     3961 tagtgctttc aaactatcat ttaatacaaa atcgatttcc gttcctttat gcataaagat
     4021 ctcctctcct tttcagtcga acagttacta gattctttta ttataccagc taaagaaaac
     4081 gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc
     4141 gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc
     4201 tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt
     4261 tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc
     4321 acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt
     4381 tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat
     4441 agcataaaga aactcttcta ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc
     4501 tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat tttttaaaaa tgaggggagg
     4561 gatcgtcgta ccgccgctag aagagttgtt tttatataaa atcgcaactc ctaaagcaaa
     4621 aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg tttctggaac
     4681 tattgcaatg attaaaggaa aaaccaagct tccgaaaagt actttaaaaa agggtttttt
     4741 acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc
     4801 tcggtcaaag cctaaagcag cttccagtaa tattcctaga ccgctgacac ctccagcagc
     4861 aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat
     4921 aatcttaatg tatgtccata taaattgtct cgtttctttc atttattcac ttccttaatt
     4981 cattaataat actcttatct tattataact tacttttcac cttaagttaa ctttaattaa
     5041 taaaaaaatt aggctagtct aaattattat gtttcattct tctagaatat acttctacgc
     5101 caaaaactgt taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa
     5161 ttcaggacac taaatagtac aggaagtaga ctaaaacttt taaaagtttt aagtctatct
     5221 atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt
     5281 gaaagcgttg tcttttgctt ctctatcact tatagtgatg acgtcatatt aaaacacaag
     5341 gaggaaatca atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct
     5401 atatacgaat acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt
     5461 atatgtgcta tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg
     5521 accaatgaaa aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag
     5581 cgaaacaagt cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt
     5641 ttacggtgat caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta
     5701 ttttcgaata ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga
     5761 aacagtatgg tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc
     5821 cgaacacact ttaccttggg gaagtaaaga tccagaccga gaagactttt tcgggggaga
     5881 tttgcaaggt gtgatggatc atttggatta cttgacggac cttggcatca atggcatcta
     5941 tttttgtccc atatttaaag ccacatccaa tcataaatac gatacgattg attattatga
     6001 ggtggatccc gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa
     6061 aagagggata cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca
     6121 gtggcaagac gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg
     6181 ttttttccca gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc
     6241 atatgatact tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt
     6301 gcaaaaatat ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg
     6361 gcgtcttgat gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt
     6421 tacagaaatc aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg
     6481 gctgcaaggg gatgaattcc atgcagtgat gaattatgca tttacagatt cgattaaaga
     6541 ttattttgca aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca
     6601 aatgctttac agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac
     6661 tgccaggatc ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt
     6721 tatgtttctt caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg
     6781 tgaagatgac ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga
     6841 gctattcggt tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc
     6901 tgaaggttct actcagtggc taatagtaaa tgatcgtgaa gataagctct atttcacacg
     6961 gaaactagag ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt
     7021 agaacaggaa aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc
     7081 agagatacga caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag
     7141 aaagcgtttg caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat
     7201 acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc
     7261 tctttaggaa tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg
     7321 gaagccgatg aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata
     7381 aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag
     7441 gacatgttcg atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac
     7501 gttatgatgg cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa
     7561 gtaaaactgg gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat
     7621 ggaaaaatct acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat
     7681 ttagtcgata cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt
     7741 tttgcttttg agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt
     7801 tattattcct atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca
     7861 aatccttcag atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca
     7921 gattggttca aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc
     7981 gccagccagc aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg
     8041 caaacataca aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat
     8101 ggacagcctt atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa
     8161 aacaaagacc tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa
     8221 ttctttgaaa tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct
     8281 aaaaacgatg aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca
     8341 aacatcccag aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca
     8401 tcaggttcaa aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc
     8461 atagaacaaa aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt
     8521 gcttcagata aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc
     8581 ttaaaagcag tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa
     8641 caagcaatat caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt
     8701 ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg
     8761 gtcagatgct tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac
     8821 caatggaaga tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa
     8881 tcattttctt gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc
     8941 gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt catacataat ataggtgatg
     9001 aaggttttcc ttatctattg acacttcccg cttatcttct catgattttt acgatcattt
     9061 tcccagtcct agttacattg ttcactgcat ttactaatta tgacttcaat catattccac
     9121 cagccagttt aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct
     9181 catatcgtaa tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa
     9241 caactttgca aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta
     9301 aattcaaacg tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta
     9361 ctatcatgag tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga
     9421 ttcctttcat aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc
     9481 cgaactggac gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct
     9541 atgtgatggt tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa
     9601 tcgacggagc aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag
     9661 tggcagcgcc aacatttatt acacagtata cggggaattt caataatttc tccatgatct
     9721 atctgtttaa caatggcggt cccggaagcg taggcggagg agctggttct actgatattc
     9781 ttatttcctg gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag
     9841 caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga
     9901 caaatgcgtt caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca
     9961 aaaacgactt tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat
    10021 ctatccgctt ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag
    10081 tttagatctt tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact
    10141 ttacgggact tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt
    10201 gactgtagta actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc
    10261 aagtttgatg ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt
    10321 ttatgtcatg gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta
    10381 tataggcggt ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc
    10441 aatagattta gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca
    10501 gattgtatta ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg
    10561 accttttggg gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt
    10621 tgctgtcggc ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc
    10681 tgctggggca attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa
    10741 ctttgttacc ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac
    10801 atttccgtta aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa
    10861 actattatca tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat
    10921 gcctgtgact cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc
    10981 cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga
    11041 aactgggtta caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt
    11101 gtctttatct gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca
    11161 atgggtgatc caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc
    11221 atctttccaa ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga
    11281 attttatgcc agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt
    11341 cctatttgtc atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag
    11401 aaaaagccaa ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt
    11461 tatgggaata ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt
    11521 gatgctaagt gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac
    11581 aagattcaaa gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt
    11641 gaaaaaaatg aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt
    11701 tgattctttt cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat
    11761 gttagagaaa actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt
    11821 aaattattgg gacggtacaa cgaaacaata cggagatacg gacactcctc cccaaatcaa
    11881 aatcaatatc catgaagaaa tcccaatgaa agagattaca agtaacgctt ctttggcact
    11941 aggagaagcc tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa
    12001 cgatgcttat cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc
    12061 aaagaaagaa aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt
    12121 aggtaacgac ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt
    12181 caatacacct gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga
    12241 caaactcttt atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt
    12301 gatgttcact gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga
    12361 acaatacgat ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt
    12421 attattaatg gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg
    12481 tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt
    12541 attgaaacca aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac
    12601 aaatgcttgg atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt
    12661 agttgataat attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga
    12721 ctaccaattg acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac
    12781 gaaagaaatg ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc
    12841 ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa
    12901 tgacatccca atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa
    12961 agaagctaag ttttatgatg ctccagaagt gaactttttt gtctaacttc taggattgtc
    13021 tctaaagctt agtttctttt tctttatatt ttcataataa aagtttggtt ctttaatttt
    13081 taaacatgac agaaaatttt ttcttcgtta tacttgtaat agaatactat tttataacaa
    13141 aggggatcga atcaatgaaa aaaactgtta tttatactag tttatttgca gctttgtttt
    13201 tagctgcttg tcaatcaaat accaatcaag gcacatctgg gaatacgtct tccagtagcg
    13261 aacagcgctc ttcacaggta tcggactcct caacaaaagc aagcacatct tcgtctagcc
    13321 agacaaaaag ttctagtgaa gcaagtactc agtcagatat caaagtgtct gcagctcgtg
    13381 cgatcgaatt gttccaagat aaatacccag atactgcaat tactagtttg gaattagata
    13441 gtgattgggg atcttacttc tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag
    13501 caagaattga tgctcaaaat gaaaaaatgg agacagagaa tccagaaact ctagatcgag
    13561 atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga tgttgataaa ttgatcacga
    13621 ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag cggtacggca acagattggg
    13681 atttggataa agatttaggt actacttatt gggaagtcaa agtaaaagac ggcaatcaaa
    13741 gtacatcagt caagatcaat agccaaacag gcgaagtact gtcaactgaa caagatgatt
    13801 aagaatttct tcggaaaaca agagttgatt tttcttttag tcatgataca ctagacaaaa
    13861 taacttgttt gaaggagcga tttacttgag tttagaaacg acctttgcca atcatatctt
    13921 tgccaatcct ttgatgaatg catctggtgt ccactgcatg actacccagg aattagatga
    13981 actggctcat tcagaagcag gagcatttat cactaagagc tgtacgatca atgaacgaaa
    14041 aggaaatccc gaaccacgct attttgatgt tcctttaggg agtatcaatt ctatgggact
    14101 tccaaactta ggtttttctt attatctaga gtatgctctt gcgtatgaaa aagtgcagga
    14161 aaaccaaaat caaccgttat ttttttccat tgccgggatg agtgtccagg aaaacttgga
    14221 aatgcttgag aaaatagaaa aaagtgggtt taatgggatc actgaattaa atttatcttg
    14281 tcccaatgtg cctggaaaac cacaactagc ttacgatttt gaggccactt atgaaacact
    14341 gaaagaagta ttcagcatct tttctaagcc gctaggaatc aaattgcctc cttattttga
    14401 ttttgctcat tttgatcaga tggctgatat cttgaatcag tttccattga cctatgtcaa
    14461 tgctatcaac agtgtgggca atggtttgta tatcgataca gaacaagaag cagtagtcat
    14521 caaaccaaaa gaagggttcg gcggcattgg cggagagtat atcaagccga ctgctttggc
    14581 gaatgttcgc gctttttata cacgtttgaa accagaaatc caaatcattg gtacaggtgg
    14641 gattcggaca ggacaagatg catttgaaca tctgctatgt ggcgcaagca tgttacagat
    14701 tggtaccgag cttcataaag aagggccgga aattttttct agaatcataa aagaattaac
    14761 gcagattatg tcagaaaaag gatatacgtc aattgacgaa tttaaaggca aattacgcac
    14821 catttcatag caaagagacg aaggtcatga ttgatcttcg tcttttttta taaaaaacac
    14881 tcgtctgttc attcctgtgc aagcgtactc attgtatgtt agacgaactt gtgggaaaat
    14941 aacagaggca aaaaggagga taatatgaat gatgcaacag aattagcaaa agagcttaaa
    15001 gaaaaagaat ttagttttca agaactagtt acggatatac agaaaaaaat aaagaagcaa
    15061 aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg agagtaattc ccacgagcta
    15121 ttgattcccc agtcagaaaa agggccattg gctggtatcc catttccttt aaaaatgctc
    15181 ggtcaagaaa aaaagggatg gctagctact tcaggatctc gtttatttga aacacaccga
    15241 gcttctcata catctaatta tgtgcggcaa gcagaagcga taggtctagt tccttttgga
    15301 caaaccaatg cgccagagtt tggtttcaaa aatattacag acccagtcat ctatggccca
    15361 gcaagaaacc cttggaacct tgaatgtaca ccgggcggat caagtggagg agctgctgct
    15421 gcagtttcat caggaatcgt tcctttagct ggtgctagtg atggcggtgg ttctattaga
    15481 atacccgctt cgttttgcgg attgattggg ctgaaacctt cgcgagggac gatgccagtt
    15541 ggtccatatg cttggagagg atggcaagga gctgctattg atttcggctt gacagtttct
    15601 atgagagaca cggaggcttt attttatggg atgagaagta tccattccgg tgcgccatat
    15661 caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa aacggttgaa gatagctttt
    15721 tgtgttgatt caccgatcca ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa
    15781 gctgtagatt tccttggaaa agaagggcat gatttgacag agattcctta cccactagat
    15841 ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg cagaaacagc agcaatgatc
    15901 cgctcgattg aaacaggcat ccagcgagct gtcagaaaag aagaacttga acccatttct
    15961 tggactttgt tccaatatgg tcagaagctc tctgccgcta cttacatcga ttctctgcat
    16021 gtatgggatg aagcagcaat tgtcatggaa aacctacatc aagaatacga tctattcctt
    16081 tcaccaacga ctgcatttac cgcccctaaa atcagcgaag atttgcaaag tgatgcgata
    16141 agagcacaga tggaacacat cagtgattgt tctgaagtag aaggtttaga cgttgtttct
    16201 tccatgtttg aggagagctt gaagatcaca ccttatacac agttggctaa tctgactgga
    16261 caaccagcaa tcagcttgcc tactcatttg acaaaagaaa acatgcctct aggaatccaa
    16321 tttatggctg ccagaggaag agaagatttg ctatttcaaa tcggctatct atttgaagca
    16381 aatcatcagt tccatttacc aaatccatgg aaatagcaga aacaaggaga agaagaatga
    16441 agaaaaaaag attgtttgct ctaataacta gtgccttttt tattttaagc gcttgcggta
    16501 cagctgccgc taatcaggaa gataacagct ggagtaatgt agaaaaaaag aaagaattag
    16561 tagtagcgac ttctggcaca ctgtttcccg catcttatta taatgatcaa aatgaattag
    16621 ttggttatga tgtagatttg gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct
    16681 ttaaagaata caacgtggat ggacaaatca cttctgtttc aaaaagagaa tcagattttg
    16741 ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa attctccctt tcagagactt
    16801 tcaaatattc atttgacagt atgatcgtac gaaaaaaaga tgattcagga atccactcac
    16861 ttgaagatct taaagggaaa aaagcagctg gcgaacccaa tactagctat atgcgtttag
    16921 cggaaaaaca cggagctaaa ttagtcacat atgacaatgc cacgaatgat cagtatttaa
    16981 gcgatgtagc aaatggtcga acagatgtta ttctaaatga ttattatctg cagaaaatgg
    17041 ccgtatctgc tttaccagat atccctgtaa aaatactgga gaacgtttat ttcaatgaaa
    17101 acgaaagtgg ttttttattg aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg
    17161 tattaaaaga gatgaaagaa gatgggacga caaagaaaat atctgaaaaa tattttcaaa
    17221 ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc ggtgagtgag tgatgtactt
    17281 tcctctaatt gatttttccc tcatgtggac ttcacttcct ttcgtactgc aaggcttggt
    17341 atacacatta gggattggct tcgtgtcgtt tcttttagga aatctggtag ggttgctttt
    17401 gacagtttta ggtcttttgg attggctgcc tctgaatgta tttattcgtt tttatttatc
    17461 gttctttaga ggcattcccg cactagtcct gctgtttctg ttatattttg ggttacctta
    17521 tcaattaagt gccttaacgg cttcagtcat ctgctttacg attaccagca gtgcatttat
    17581 tggtgaaatc tatcgaggat cactagcagg cgtttcttca ggtcagtggg acgctggttt
    17641 agcttctggt ttttctttct ttggggtatt gaaatatatc gttttgccgc aagcttttcg
    17701 aatctcagta ccggcactca gtaacgtagc tatggatcta gtgaaaggta cttcgttagc
    17761 agcaacaatc acagtgccag aaatatttca aaaagcaaag atcataggcg gtcggacatt
    17821 cgattatttt tctctctata ttttagttgc actgatttat tgggtcattt gtatcgggat
    17881 cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat cagtcaatct gaaaaataga
    17941 aatggtgcta gcgtggctgg caccatttct attttttgtt tcttgtatat tctgcctagt
    18001 attttaaaac catttcatgt aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg
    18061 aagaccccgc taaccatcga aatagaatcc tcgttatacc aatattgcat cgagcaaggc
    18121 ggcttggttg tagaagaagt cacgatgcct gatgaccaag gaattgtaga tacgcttagc
    18181 tgcttgacaa aatttgatgg aagccgagaa tggcgatgct atgagctgaa agtgacaaaa
    18241 gcagattttc gctctacagc caaactgtca tttgttggac attataatta ttttgtttta
    18301 ccaagtaaac tatatgaaga agtaaaaact gaaatcccta caggaatagg tgttttagtg
    18361 tatcgtccgt acacggttga agaggagatg cctgcctctg gtacatttct gattgcgaaa
    18421 aaagcagcaa agaaagagct tgctgtaccg gaagaagctt tgacaaaccg cttcatggct
    18481 tctttattca gggaagtaag aaaagcaaag caaatggagc gaggcgtaaa atattttcca
    18541 acagatcaat tgtacaaaga attgaaaaga cgttcagaaa atcaagatcc attttctaac
    18601 gaacgatatt atgaacggtt tttagaggaa atagctagtc aggcaatcaa tgagttgcaa
    18661 gaagaagtta tcgcgcttca gcatgattat gaatatttga ggcaacaaca gcagatccgt
    18721 cggttgccta cagagccatt ggagtgaaaa atatgtacta tccttattta agaggaaaac
    18781 aatttgacct attagcatta aaagaagcac tgtcgcgagg attgctctcc aataaaatcc
    18841 aaccagtgat cgaaccagta cgtgattccg ctacgcttaa aaatgtgatc gaattatttc
    18901 aaaaaaagca ccatccaatc gctgtgatcc aaaatcccca agtaggacaa ttcaaattat
    18961 tcgatcagca tgtgcacagt tggacagtca aagaaaatag ctctgtcgtc cctgcacaga
    19021 tcttaacgcc agaaaatctc agtgaagtac tggattcacc tccagcgttt cttgttttcg
    19081 atggacagca ctacccaaaa gatacagagg tgtggaaaca gttggcagga gttgattcga
    19141 aatttttgat accagatact agtcgctttc gcatctggct gcccgaaaac aaaattgtga
    19201 tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta tgcagataaa acagatgact
    19261 tttttagtga tgactatctt tttttccacg cagacggata tattggattt tctgatttca
    19321 cgatcgaagg tagccgttat ttcgataaag ggttccctag ccgagcgctt gctatccatc
    19381 taacgtatat tgatgcttac ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg
    19441 actctgctaa agaccaagca ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga
    19501 tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat gattgaatta ttggctcttt
    19561 accagcaaca aaaattccct ggactgggag tgttgaaaaa gtggtcactt atgcatcatt
    19621 tggaacttat tagtcacctg ctagatcatc caaaagattg gcttaaatat tattcaaaag
    19681 ttcaggaact atatgaagtg atccaatgat tagataatgt aaatgaaaaa ggaatagaaa
    19741 agaggaaact ggatgtatat tcggcaaaca aggaaagaag aagtagaaga agtggtagaa
    19801 atcattaatg ccgcagcgga tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg
    19861 cagaaaccaa caaaagaaac gattttgaac gatattgaaa gaaaagaaag ctatgtatta
    19921 atagtggaag atcaaattgc tggaacagct gcattagtct caagcgtgga tcccgtttac
    19981 acaaaaatag atggtagttg gaaagaatca actggtcctt atttatcgat tcatcgtgtc
    20041 gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg
    20101 acgattgcag ccagcaaaaa tatccaagat atccgtatcg atacttatcc agctaatgaa
    20161 ccgatggaaa agaccatcta ttctgctgga ttcacttatc aagggatgat tgaatttcct
    20221 tttgaacacg gcgaaagaaa agcatatcaa ataatcatca attgactata aaatcatctg
    20281 agcaaagata aagacattaa cactactgaa aaaaacaatc atatgatagc caagaggttg
    20341 taacagaagt ggccagcttc aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt
    20401 ttgtggaatt ttagcgtatt ttacgaaata gctgctttct gaaactccct tcatcgagag
    20461 gtcgtgacat agcttttgtc acgacctctc ggcttatcct acgagtatta ttcgatacat
    20521 ttatatcaat aatccttttt ttattttatc tgcagcttct tggaacgctc catcgtttga
    20581 cacgactaaa agtgagggga tagctttcaa acgtttcaac tgtgctaaat cttctacata
    20641 aaccgccagt ctggctgaaa tctgttcaga tgaatctcca cgttttttca ggcgcgattg
    20701 caatgtttct ttagagatcg taataaagat aggacggact accgtcccga aatactcata
    20761 taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt
    20821 tttaatttct gctttactgg aaccatattt atgttgagca tattggtccc actctgcaaa
    20881 ctcatttccc ttgatcatct gattgaatgt attgtcagaa acaaaataat aatcctcttg
    20941 atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta atgatctttt gttccggctg
    21001 aaaaatagct tctgcgagtg aagtttttcc agaaccagaa ggaccgataa atacatagca
    21061 ataattcatt gtcatttctc ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt
    21121 tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta taccagcaag ccagtcacaa
    21181 tagcacttaa aaagctgact aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga
    21241 caagatttcc tttttcttca tttaatccct tcatcgcacc agaaatgatc ccgatagaag
    21301 agaagttggc aaatgaaacg aggaagacag atataattgc tgttgtacgc tcagtaaaat
    21361 ggaaattacc atttgctaat tctgtcatag caacaaattc atttgtaacc agtttagtcg
    21421 ccatgatact accggcatcc actgcttctt tccatgggat tccgctgatg aaggcaagag
    21481 gggcaaagat gtaacctagg atctcttgga aagtgatgcc gaatatcccg tggaagatag
    21541 cattgatcat ggcaatcaaa gcaacaaaac cgataagcat agcagcaacc gtgatcgcca
    21601 catggaaacc atcaagaatg tattctccca acatttggaa aaatgtctgt tctttgtttt
    21661 cttcaatgat cagctcatct tctttttctt ctaatacata gggatttaca attgaagcca
    21721 gaatataacc gccaaataag ttcaaaacaa gggcagtgat cacatattta gaatcgatca
    21781 gtgccatata agatccgacg atcgacatag aaactgtaga catagccgat acactcatgg
    21841 tgaacaaacg tttctcagat aggaagggta attccttttt tatggaaata aaaacttccg
    21901 attgtcctaa aatagcagaa gctactccat tataagattc caatttgccc atcccgttga
    21961 ttttacttaa tcctagacca acagctttca taaataatgg aagaatcttg atatagcgta
    22021 gaataccgat aatagcagaa ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg
    22081 cacttccttt atttgctaca ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa
    22141 gaagatgatc aaacattttt gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta
    22201 ataatacgaa accaagaaca agctgtaaaa cgaacatcac aattagtggt ttcaatttga
    22261 ttgctttttt gttactgctg atcaaaaaag caagccctaa tgtgacaaat aaaccaataa
    22321 tacctaataa ataactcaaa taatcactcc tattccaaat tccgttttat tttacctgtt
    22381 ttgcaaataa aatactaatc tttagtgaaa ttttcattac ttttttagag tgaaacgcat
    22441 tcattttcca ttagaaaata aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta
    22501 gtaaagtcta taaaaaaacg aaactatcga ttaagctaat agtttcgttt tcaacattta
    22561 taagtaaact ttttctattg gtaatataga ttttcggaag ggtagacagg tgcgcaggta
    22621 atatcgattc ctagtttttt caatgtttgt tcatcatttt tattgaccat cacagtagag
    22681 tgggcttgtg ctccctcaag ttcagctagt ttatcataag caacttgagc gattggattc
    22741 gttactgcac tgattgataa agcgatcaag acttcattga tgcttagtgc atccaaagag
    22801 gaatgaagtg tctttgattt caatccttgt attgtttcaa gaactaatgg tgacaataaa
    22861 ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat ttaagataac tgccgctgac
    22921 gcatccatca atgaacttgt acgcccggta atgatgactt catctggtaa ttcgattgca
    22981 atcacagcag aaggatcttt tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg
    23041 gcagggacta ccacgtttcg gtcttcagga cgtaaaccga cttcttccat aatcagtttg
    23101 attcgattaa ctgcttcttc gtcgatcaat ccttttttga agtcgcattc tgtttggaaa
    23161 taacgacgga tgatttcttg tttagaggct tcttggacaa cggtatcatc cacgatccca
    23221 aatcctacac gattcacacc catgtcagta ggagattgat aaacagattc tttaccagta
    23281 attttttcaa taatacgttt gattacagga aatgtctcaa catcccgatt atagttcaca
    23341 gctactttat tgtaggcatc aaagtggaag gaatcgatca tattgacatc ttttagatcg
    23401 actgtggctg cttcataagc aatgttcagc ggatgtttca acggaacatt ccatactggg
    23461 aaagtttcga acttggagta accagctgca tgtccttttt ggctttcatg atacagttgg
    23521 ttcaaggaag tagcgagctt tccacttcct gggccaggag ctgtcactac aacgattggt
    23581 ttagttgttt caatatactc attttttcca aaaccatctt ctgagacgat tttttctacg
    23641 ttgactggat agtcttcgat ttctgagtgt ttatagacct tgatgtttcg tctttctagt
    23701 ttattgataa agactttcgt ggcaggctgt ccactatagc gagtgataac gacactgttg
    23761 attaataagc cataaccacg taattcatcg attaaccgca agatatccat atcataagta
    23821 atcccataat caccacggat cttattacgt tcaatatctc cagcatagac gcaaatcagt
    23881 atttcagctt gatctttcaa tttttgcagc aatttgattt tcgcatcttc ttcaaatcct
    23941 ggaagtacac gtttcgcatg cttgtcgtca actagcttcc caccaaattc taaatataat
    24001 ttgtcatagt ggttcactcg ttccaaaata tacgctgact gctcttcaat atatttttct
    24061 gaatcaaaac caattttttt cactaggttc cctccttaag tactaaagca ttttttcatt
    24121 tttttgagga ttcgtcaact ggaagagcga aattcctcaa aaaaacaaag aaaatggttt
    24181 aaaccaactt gtctagatag ttattcattg gtatgatagt ttttgataag gaaggtgcta
    24241 catgaaaaaa agacagaaaa agaaaaacgc atataaacac tatatccgtt cgatttttac
    24301 cggttatgaa agaatgcttg aagatcccga attggaacaa ttgacgttta cttatctaaa
    24361 cgaagaaacg cagctaactc gtgatgagca tcagcgcatt catttcacaa caagagatct
    24421 gcctagtaaa taaaaaataa gataaaggtc atcggattcg attctgtcct atcagatgga
    24481 aagactagtt gatcgaaaaa aaggctgtac caaattttgg tgcagcctgt tttatggagc
    24541 attgcccgct cttttttata acaatttttt acagaaaatg gtactattga agtattaata
    24601 agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa agagccagat acaaatgcaa
    24661 attatgagcc agacgaacta gaggagacga atgcggatca ggatacagga caagaatcgt
    24721 cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct ttcccaatta ttagaagact
    24781 tacaggcttt tgaaaaagct gtccgcgaag aagatatccc tgagatctac cgtttataca
    24841 atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat
    24901 tgctgatgcg taagatccat gatcgttttt tacaggaatt tccttttatg gaacaagtag
    24961 aaaacatctc gccaacgatg agctattaca aaatcggcac ttattaccat gatcggccca
    25021 cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt gcctaagatc gatgaagagt
    25081 gggagaaata ttcccaaccg gagtcaacgg actatgacaa aaaaatcaat gagctagacg
    25141 caaaggtgat cactgctcag acagaaattg aacgtttaga tgaacaaata aaagaaatca
    25201 atcgacaaat gactgatttg aacgacaata aaggattctt gaatcgtaaa aaaatcgagg
    25261 aagaaattca agaattagag aagaagaagc aagtactatc taatgaaaaa ttaggctggc
    25321 ttccttatat tgagacgcct gaaacaatcc agcagcaaaa agaagcattg aagcaagaag
    25381 caagagcaga ccagcttcgt gcagccatcg tagaaaaaga acaaaggcaa atcagacgct
    25441 atttcggtag caaagaggga ttcggtcaag caatccatga attcttgatg aattatcttg
    25501 gcaatgagaa tcctaataat caatctaatg aaggaggttc cgagtatgaa tgaacacgat
    25561 gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc atgaagaaac gaacacttca
    25621 ttggaagaac aaacaagtaa tcctctggat gacacagaag aaatccctat gctagaggaa
    25681 acaaaaatac acgaaaaaga aaaagtaact ccgacgaata cgaatactgc agaaaacacc
    25741 catgcttacg aaataactga aaatcccggg tatttagaag gaaaagagca agatcatcaa
    25801 gatatcgtca gtttgattga aacagcggaa gcagaattag cggatttacg tatgagaaaa
    25861 gatttgatcg aatcagattt tccacaatta ttagaatcat atgatcgttt tatattgaat
    25921 gaagaaccaa tctccacgat cggaaagaca agcctgatcg aatacttcac gtatgaatta
    25981 ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt cagcttatga cacatggaaa
    26041 acaagacatt tttcgatcaa ttatacgttg aatggacaaa acgaattagt cttttccttt
    26101 gtattaccga ccatcgatca acgataccaa gtggaatcga tgagtctcct aaacgtatca
    26161 ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc tttctttgat ccgacactgg
    26221 tcagtggatc gagtattctc tgcagggcag atcacgatat tcaatcacaa attgaatcaa
    26281 ttattagccc atgcaagaac acttggtttt accgttaatc agacattact agacaatacg
    26341 aagccacttc gtttgaatct tcagagcgaa tttgaattga cgaatgaagt attggatgat
    26401 atttttatcg tggcaatgaa aaatccatcc tacgatatgg aaaagatcaa cgaaactact
    26461 tatcaagtac gattagataa aggacagtct ttaacgataa caacaaacga aaaagatcaa
    26521 acagtcttgt cgatctcatc tggagactac ccacgatctg tcattgattt ctttatcaat
    26581 tacgaattct tggtcccatt aatggtgaga aagtcctgat tggataagtt ggattaccag
    26641 atgtcattca gataattctg ttccaaagga cttgcggcag ttatccagaa taattgtcac
    26701 aagtcttttc tgtatgtact aaatggacgt gaataacttg ctgatgaagg agggatttca
    26761 ttaggaatgt ccacaaataa aatagcacag cttagcttat tgtctgctgc ttgtgtagca
    26821 ggtaggatcg cttttcaatt tattccgaat ttccagccaa tgactgctat ttttttattt
    26881 atagtacttt acttaagctt aaaagatgcg ttaattgtca tgagtctttc tattgcgatc
    26941 agcagttttt attttggtgt tggaccatgg gtcatcggcc aatggatcag ctatactgtt
    27001 gttctgagtt tgttttcaat cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta
    27061 aagggagtct gtttttttct ggcgggcatg gtttacggaa taggtatgac cgtttttgat
    27121 acgctgttat atcgtttgcc tcaaccttgg atctattacc ttcaaggggt gtcgtttgat
    27181 ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt
    27241 ttttacaatc attcaggagg aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag
    27301 tgtcagtata ctcattttga caagttgcag tacaacctat acacaatcgg cttccaacca
    27361 aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa
    27421 cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg
    27481 aaattttgaa gtcaaagaag ataacggctt catcacttcg cttgaagggc actcacagga
    27541 tgagaaaaat caactctatt ggatgtttac tgtggacggg aaaagtgcta ctactggtgc
    27601 caaagagatc aaattgaatg atggacaatc ggtcattttc aatttgtcta aactataagt
    27661 ttcataaaaa actgttaaag ggcgaatatt gccataattc agtgagttca gttgatgaaa
    27721 gaaggaaaat aacatggaaa attttaaaaa aagcggaatg ttttccgtaa ttgctgctct
    27781 aggggctaat attctagttg ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc
    27841 cgccatgcta aacgaaagca tccacagtat cgttgattgc agcaatcaga ttttgctgtt
    27901 attcggtgat aaacaagcca ataaaggaca gagcgagttg catcaatttg gtgaaggacg
    27961 agcaaaatac tttttcagta caatcgtcgc aatgatgctt ttcttcggcg gtggggcact
    28021 tggggtgatg gaagcttttg aaaaattgac gcatccatcc catgaagtgg gtaatccgat
    28081 cattgttata gcaatactgt tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc
    28141 aatgaaagaa atcaaagaat tgaatactga aaaactttcc acgatgcgct ttttgcgcga
    28201 aagtcgacac agtgaaatac tgattatttt tacggaagac ttgtgtgctg taatcggttt
    28261 ggtcttggct ttagcaggaa caatattgac ttatgcaacc gggattgctg catttgatgc
    28321 gatcagcgga ttgctgatcg gtttcttgct gatgggagcc gccattttcc tagcaaaaga
    28381 attttatagt ctgatcatcg gtgaaagcgt gacagcaagc gatttgtcaa aaatcaagtt
    28441 agcctttgaa cgacctgaaa tcagtcggct aattgatatc aaaacagttc atttaagtcc
    28501 aacggagatt ttagttgcag caaaagcaga tatcgttggt aacaaagaga atgaagctta
    28561 ctcggtcatc aacgatattg aaaaaaatat gcgtcagtct cttcctgaca agaagatgta
    28621 tatatacatt gaaacagata aatatgaccc aaattattct cgtaaataaa acgtttattc
    28681 atgaattgaa gcaaaaatga gaaaaatata atcttgtttt taaataaaac ggctacaatt
    28741 tagcgaattt atgttacaat gagtccgctg tttatttgtt gtaattaaag gagatatttt
    28801 aatgaacaaa aagaaaagtg cgccatggct tcaagctttg catgcagtga tgccactttg
    28861 tataagctat ataccagtag gattagcttg cggagtattg ttgcaaaaag ttggatttga
    28921 tcctctctta tcaggactgt tatcgattct ggtcttttcc ggtggcgcgc agtttttagt
    28981 tgcatctatg cttacgacgc aagcatcatt tgcaacgaca ttattgatgg ttttcttttt
    29041 agaattacgc tatacattat taggagcaag tcttgccggt tttatgaaaa aagaaaagcg
    29101 gacgtttttg gctgtctttt ctcaatcact gaatgatgaa aactatgcgg tgaactactt
    29161 aaagttttca actgaccctt cctggaataa aagaaaggct ttatatgtca attggttctc
    29221 aatgactgca tgggccggta gcaatatgat cggtaatttt ttcggctcgg tgattcgagt
    29281 agatgctgac cttgtccatt ttgctttgac ggccatgttc attttcatgt ttatcatgca
    29341 gatgaaaagc gcactactga tctttacagg tttattctca ggcgtattag gggtcgtatt
    29401 catggtatta ttccaaaata ctttcggatt gattgcagca acagtcattg cttcatttgc
    29461 cggatttatg ctggagaagg cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg
    29521 taaagatgtc aatgagccgc tcttcaactt cccggaacag gaggccaatc accatgacta
    29581 aattatattt gctactttta gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt
    29641 tcccgatgct ttattttact catcgaaaag tgccatcctg gttcagtgac tggatgaaat
    29701 atgtaccggt ggcattattt gcggcgttag cttttaaaga tgttttcatt actcatgaac
    29761 atctggatat tgcttggaat atcaaaatag ctgcaatgat tctagtagca ggcgtggctt
    29821 acaaaacacg ttcgatggcg ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt
    29881 ctatgcttta aactgtttta ttttattaaa aacagagatt gcgacataag ttgttcaaac
    29941 tgatctatcc gaactgcatt tgcaacagtc aattctggat gcaagtttat tcgtagggat
    30001 aaaaaagatt agtgttggct tatgatgcaa gctctttttt attttaggaa ttagagaggg
    30061 atttgtcctt ctatgatttt ttgctataat agggaagatg tttattttat gaaagaatag
    30121 ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac attttggcta acctttgcta
    30181 atcttctatg taaagtttta ggggtagtct atttgattcc ctggctaagt atgatgggga
    30241 acaatcaaga tggtatgctt gctacgacat tgtataatgt tggctatctt ccttatggat
    30301 tgttcctgat gcttggtaca gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag
    30361 ccacaaaaga aggagataac gctgcttgtc ggctgatttt tagaagtacg atcaatatca
    30421 tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt atttgctcct ttactagccg
    30481 gaatcagtcc gattgctaac gttaataatg ggatactagc tatcagaagt ttatgcccat
    30541 ctttgattgc tatcccaatt cttagtgcaa tgagaggata tttccaagga aaaaatgatc
    30601 tgcggcctta tggaacttct ttgattatcg aacaggctgt gcgagtgatc gttatcttgg
    30661 ctggtactta ttacttgcgc gtattgactg acggaaccat attagaagct gtattgatca
    30721 gtacagtcgc ttctttcttc ggtgggttag ctgcgattat ccatatgtat atcgtcggac
    30781 gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc cagccgttat ttcgaaagag
    30841 aaaaccgatc tgcctctgtc tctatcattc gggaaacact accgtttatt tttgtcggct
    30901 cagtcattac tatcgttcaa atgatcgacc aagtaacgat gaaaccactg cttcactttt
    30961 tccgtccaga gatcgcagat cagcaactgg aattcttatt cagccgtgct tctgtcaatc
    31021 caaataaatt gacattgatc ctgatctcca tggtaggaac agtagctata agcagcttgc
    31081 caatattaag cacgatgaag aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata
    31141 gtttttcgat tgcgttattg attcttcttc cttcattgac tggaatgtct ttattagctg
    31201 gaccactata cacattgttt tttggctatg atcctgaaag tgtcggatat ttccaaatgg
    31261 ctttgctagc ttctttgttt ttctctttat tcacgatcca atctacgatg cttcaatctt
    31321 tgaatcacca tcgttttgcg atccgattaa ctgtagaagc aattatcctg aaagtcatct
    31381 tcgaagtaat cggattagga ttagttggag gatacgggat gagcttatca aatggcttag
    31441 cattcggtat tgtctttgtt cgcggctatc actttatgtg caaagaatac cgaatagcac
    31501 ctttagcaaa gatcagcaat tttttcttaa aaactttccg ctccaccctg attatgcttg
    31561 cagtatgttt tgccgttttc tttttgctga atcagcgatt gtcaatggaa tcaaaaagct
    31621 atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg cttagtattt ttattttctc
    31681 aattcggaaa aaatgggttt cgaatgttga aaaatgttaa acatcatagc agaagcaaag
    31741 aataggttaa ctaatcgttt tttgctgtca cttgaaaaac ggcaactcgt atgattacgc
    31801 ttgtcgtttt tctattttcc aattttagtt tcatatctaa tcttacttat gatatagtta
    31861 taaggtctgg aaaaattctt tgaaacgagg aaattaaaca catgaaactt gcagtagtta
    31921 cagatagttc ggcatatctg ccggaacgta tcaagaacca tcccgactta ttcgttattc
    31981 caatcccttt gattatggat gggaaaattt ataacgaagg aatcgatatc gaagcggatg
    32041 aatattatgg acttttaaaa aacagtaaag attttcctac tacttcacaa cctgtcgtag
    32101 gggaagtttt gggtttatat gaggaattga aagagaaagg gtatgacacg attttaagta
    32161 tccatctttc ttcagggatt tctggctttg tcaacacatt atttgcgatg aaagatgaca
    32221 tcagcggagt aacggtcatt ccttatgact ctaagatcac tagtatgccg atgggacaca
    32281 tggtagaagc tgccttagac ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac
    32341 atgtcgatcg aatccgagat aatacctacg cttatctgat tgtagatgat ttgaacaatt
    32401 tagtacgagg aggacgtttg acaaatggtg cagcgctgat tggcagctta ttaaaaatca
    32461 aaccgatcct gacattttct gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta
    32521 aaaaagcatt tgctcgagct gaagaaatta ttggtagaag aaataacgaa atacaaccgc
    32581 cagtcaaact ttacgttata catgcaaata atttagaagt agcgattgcg gagaaagaga
    32641 aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg
    32701 gtacacattt aggtgaaaaa gccatcggca ttgccatttc agcgcaataa ttccttttat
    32761 tcaaaaaaca taccaaaaat attccaacat catcttggac tttctacatg ttactaatga
    32821 gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaac gaagctcttt ttctttgcaa
    32881 tccacacttc aatcctctat aatgtcgcat atagtttagt aaatgagtaa
//
LOCUS       DAGCWU010000014        32520 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.014, whole genome shotgun sequence.
ACCESSION   DAGCWU010000014 DAGCWU010000000
VERSION     DAGCWU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.014"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="MBZ71_000534"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="MBZ71_000534"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HBT4428511.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="MBZ71_000535"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="MBZ71_000535"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HBT4428512.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="MBZ71_000536"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="MBZ71_000536"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HBT4428513.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="MBZ71_000537"
     CDS             complement(4028..5044)
                     /locus_tag="MBZ71_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4428514.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="MBZ71_000538"
     CDS             complement(5146..5343)
                     /locus_tag="MBZ71_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428515.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="MBZ71_000539"
     CDS             complement(5315..6634)
                     /locus_tag="MBZ71_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBT4428516.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7375..8355)
                     /locus_tag="MBZ71_000540"
     CDS             complement(7375..8355)
                     /locus_tag="MBZ71_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBT4428517.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="MBZ71_000541"
     CDS             complement(8368..9246)
                     /locus_tag="MBZ71_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBT4428518.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..10062)
                     /locus_tag="MBZ71_000542"
     CDS             complement(9262..10062)
                     /locus_tag="MBZ71_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBT4428519.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(10337..10486)
                     /locus_tag="MBZ71_000543"
     CDS             complement(10337..10486)
                     /locus_tag="MBZ71_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428520.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            11454..11780
                     /locus_tag="MBZ71_000544"
     CDS             11454..11780
                     /locus_tag="MBZ71_000544"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4428521.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            11761..12696
                     /locus_tag="MBZ71_000545"
     CDS             11761..12696
                     /locus_tag="MBZ71_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBT4428522.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            13325..13576
                     /locus_tag="MBZ71_000546"
     CDS             13325..13576
                     /locus_tag="MBZ71_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HBT4428523.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            14118..14276
                     /locus_tag="MBZ71_000547"
     CDS             14118..14276
                     /locus_tag="MBZ71_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4428524.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            14348..15574
                     /locus_tag="MBZ71_000548"
     CDS             14348..15574
                     /locus_tag="MBZ71_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4428525.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="MBZ71_000549"
     CDS             complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="MBZ71_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBT4428526.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16071..16514)
                     /gene="rplM"
                     /locus_tag="MBZ71_000550"
     CDS             complement(16071..16514)
                     /gene="rplM"
                     /locus_tag="MBZ71_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBT4428527.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(17033..17500)
                     /locus_tag="MBZ71_000551"
     CDS             complement(17033..17500)
                     /locus_tag="MBZ71_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBT4428528.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(17620..19158)
                     /locus_tag="MBZ71_000552"
     CDS             complement(17620..19158)
                     /locus_tag="MBZ71_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBT4428529.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            19238..19924
                     /locus_tag="MBZ71_000553"
     CDS             19238..19924
                     /locus_tag="MBZ71_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBT4428530.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(20125..20652)
                     /gene="msrA"
                     /locus_tag="MBZ71_000554"
     CDS             complement(20125..20652)
                     /gene="msrA"
                     /locus_tag="MBZ71_000554"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBT4428531.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(20736..21413)
                     /gene="pgmB"
                     /locus_tag="MBZ71_000555"
     CDS             complement(20736..21413)
                     /gene="pgmB"
                     /locus_tag="MBZ71_000555"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBT4428532.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(21410..22540)
                     /locus_tag="MBZ71_000556"
     CDS             complement(21410..22540)
                     /locus_tag="MBZ71_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBT4428533.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(22540..24723)
                     /locus_tag="MBZ71_000557"
     CDS             complement(22540..24723)
                     /locus_tag="MBZ71_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBT4428534.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(24720..25745)
                     /locus_tag="MBZ71_000558"
     CDS             complement(24720..25745)
                     /locus_tag="MBZ71_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4428535.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(25790..26617)
                     /locus_tag="MBZ71_000559"
     CDS             complement(25790..26617)
                     /locus_tag="MBZ71_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBT4428536.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(26614..27681)
                     /locus_tag="MBZ71_000560"
     CDS             complement(26614..27681)
                     /locus_tag="MBZ71_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBT4428537.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(27707..28543)
                     /locus_tag="MBZ71_000561"
     CDS             complement(27707..28543)
                     /locus_tag="MBZ71_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4428538.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(28540..29409)
                     /locus_tag="MBZ71_000562"
     CDS             complement(28540..29409)
                     /locus_tag="MBZ71_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4428539.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(29479..30762)
                     /locus_tag="MBZ71_000563"
     CDS             complement(29479..30762)
                     /locus_tag="MBZ71_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBT4428540.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(30776..32461)
                     /locus_tag="MBZ71_000564"
     CDS             complement(30776..32461)
                     /locus_tag="MBZ71_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBT4428541.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
BASE COUNT         9176 a         7025 c         5763 g        10556 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt
     9841 tgccgagatg gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt
     9901 taatagtttt tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac
     9961 ttcatatcct gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc
    10021 tgaaaaatca tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta
    10081 tttttagtag ctactacggt aacaactata aattcttttt gattatctgt caagagttag
    10141 ttcaataaca cacatataat aattagctta aaaacaatct ttttattaaa aagaaacatt
    10201 ataatatgaa tgtttgtgaa aaaataatac aacttagtca ttctttacaa cgatttatag
    10261 aaaaacaacc tctttaaata taaaaggtac gggttactga ttacttgtaa atctttacgt
    10321 tccagtttta ttctttctaa aaaatatact cacttctttt cttagacttc tttttttgat
    10381 tctcctcttt atggtcttgt ccttttttgc cttcaaaata tttaaataaa cccctttcaa
    10441 aagcatttct aggttttgga caataaggaa tgactttttt cttcatataa atacctcatt
    10501 taactatttg atatcatcac attatcaaaa aaaataggtt ttttattaaa actatcttga
    10561 cctttcggga gatttcactg tgatttattt ttattcccat acttttttcg ttggtcactt
    10621 tcgtatacct aatctaagcg atcatcggcg acggtgcttt tacaacgaat ggcaggacaa
    10681 aacaaggggc ggaaaaactt ttttgcctat ttcataaaaa actttttcct acagcaatcc
    10741 cttgttttgt cctaccacac gttgtgaacc caaagcacgc cgacgaacgc ttagctatac
    10801 gaaaatgacc tccaaaaaca aaaaagtgag gtgaatagaa acaaaaagaa attgaaatct
    10861 cccaaaatgt cgtccgcctc ggagacaact tgctgctgaa aatggttggt taattcattt
    10921 aagtattaaa taagataaat catcaaatta atcggaaaaa tagcatgatt ggccttatca
    10981 aaaataaact gtaaaaactt atatttctat gagtggtgga acaatgctaa taccaaatta
    11041 atttatcgat tacccacctg cacaaaccaa agatagcatt attaggaaac gaatcaatca
    11101 gcgaatccag cattataatg aggatactaa aaataaagtt ttcataatta taaatccgcc
    11161 ttcctacgaa aggcaccaat gaggtgatga aaaccttaga gcgctttctt tcttttttta
    11221 ttccgtgtta ccctttcctt gcaggtccct ggtacactac agaacccgag gaggtgagtg
    11281 gcggggctgt atagcggcaa ccccgcataa ttatatggtg tcggtcatcc gttaaggcat
    11341 caatgaatgg cgcaaacccg tagcccgtaa acttatctct tccgaagttg actcgatttc
    11401 gccggatgac cagtccctac caaacctgta ctattttttt acaggaggtt gctatgttca
    11461 aaatctttcg taaaaattgt tgtggcttgg atgtccacaa aacgtggatc tatgcgtgta
    11521 tcggaatcac agatgcgaat ggtcgtacag tgtataagca agctcgcttc tcttccttta
    11581 caaaaggtct caatgaatta gctgaatggc ttgctaaata caactgtaag gatgtgtgtt
    11641 tggaatcttc cggtaaattt tggattcctg tttttaatat tcttgaaaaa acttgttttg
    11701 tcacactggc tcatccgaag tacaccaaac cccagaaagg aaataaaaca gatcgcaaag
    11761 atgcaaaaat ggatttgtga tctctttatg tgcgacatga tcaaacccag ttttattcct
    11821 ggtcttgaaa tccgccagct tcgtgatcta atgcgttatc ggacgaaact gaccaacatg
    11881 aaaacaagtg aaaagaatcg tgctttaaac tgcttaaccg tttctaatct gaaactagat
    11941 gatgttttta gtgatgtgtt cggaaaatca tccacttcta tcatccaata cttgttagaa
    12001 catcccggtg aatcatttga tgttcgacca tttatccatg cccgttgtaa aactcccgtt
    12061 gaagaaatcc aagcggcagt tgagggggcg atttctgtac ctcaagcgat taagcttcgc
    12121 caatgcctca accatattaa cgagattgag aagcatcgag aagagatcaa tctagagatt
    12181 caagctcttg cagaaccctt ctcccctgtt ttggaactac ttcgtaccat accgggctta
    12241 gacactcagc caataacagc gattgcgatt ctttcagaaa taggaccgga tatgtccgtg
    12301 tttccaactt cgaaacactt tatttcttgg gctggatgct gtccaagaaa cgatcgcagt
    12361 gcccgacgaa caaaatcaac acgtatctct cgtgcagggc agtcgttgaa accgctactt
    12421 gtacagattg ccaatgctct tctcagatcc aaagaacatc cagaatttaa aaatcgctat
    12481 cgtagaatca aagcgaatcg tggacacaag aaagctatca ttgctatttg taagatgctc
    12541 ctgaccgcta tctggaatgt cttatcaaag gtggaaccct attctgctaa aggatatctg
    12601 gagccaaaac ctgcgaaaga agagaaagtt ctgacgcaat cccaagctct ggacctttta
    12661 agaaaacgcg gatataccat tatagatagt gcttaaacca aagttacgtt gattcttcct
    12721 aaatagactc gttgtcttcc atttcaaaaa ttttgaaaac ggtaggctta tttagtgtac
    12781 cgttcttcag gtatcttcaa atagatttat tttcaaactt atttcctttc ttcttttact
    12841 ctcctatagc ttagcattta agtgggcact cgtgtcacct tgctagatag ctatgaatgc
    12901 cgtaataagc ctcctagtat ataggcagac tgccaattat aactgattca atccacatta
    12961 tgataaaatc caccttttcg tttcatatgt gttgaaatat cacatatgtc aaaagtgcac
    13021 gagtcttttc attcagtgct ttgtaaatac tcaaaattat tccttttcac tttaaaaaaa
    13081 agataattag tacaaaatca gaaatgagga aagtattagg atggactaga aaggagttgt
    13141 tgaatttgtt gcaacagatt ggtcatttat caattaaaaa ttaggtgaag ttttattcat
    13201 cactgcagct gctgtttccc agagaataaa acgtctagaa gaaactggtc agttactgta
    13261 ttatcaagca gcaattgact acgaacaagc ttgattacta accaaagcgt ttatccacct
    13321 gttgattaaa tcaaatcaaa aatcaggttt ttatacgttc ataaaagcaa tctccagtgt
    13381 actcgaatgt gactgtatca taggtcctta ttcgatgtta atcaaaatgg ggtttccttc
    13441 taccgaatca ttggacagtt ttatcaatga tttatcaaag tttggacgaa ccaacaccca
    13501 aatcgttttc tcgactcttg tttccttaat ggtttatcag ttctggaatg tttatcatgc
    13561 gcttttatta aaatagaaaa gagctttttt catgcgacaa agcagtgatc atttattggc
    13621 acatgcaatc tttgtggatc cattttttga aaatacgata tttagttttg acccaaaaat
    13681 ctttaaatac gtatttgata atttatctta acaaccagat aattttattc agcaccgtat
    13741 tatctatatt gaattctcgg aatcaagata aatttttttc cgtttttgtt tcttttttaa
    13801 tcttcttctt gttattaata gtgattagga tgatattcct atattgatct ttgataatta
    13861 gatataattt ttgaataacg ttccctgcta ttcgagctag aaatacaagt acgccatgac
    13921 ctagttagcg acaactactt agagatttct tttgttctgg gacaacaaaa tgccatgaca
    13981 atagcagtaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc
    14041 ctatgtgctt acccaaagcg gttcaaaaat tttaaactat tattttagga gatgatttta
    14101 taaaatcgaa agcctacttg aataaaaaac aagcctgtga atttctagga attgataata
    14161 atactttaga taagtggatt cgtgtatata attttccagt aataaaaatt gatggcgtag
    14221 ttcgctttga cgtagaggat atcaaagatt tcatgaatac attcaaaaaa aattgaaatt
    14281 ttcttctcgt cacatatact ataaatagcg aaatgtactg ccttactttt taaaggagtg
    14341 aggaagtttg cctacaaagc tttcaaatgg gaaatataaa actaatctga ggtatccaaa
    14401 aaaattcaga gaaatcactg gaattgcatc agagaagtat caaaaagtat ttccaactag
    14461 gcaattagct attaaggccg aaagggaaat gaaaaagaag attgaaacag ttttaagaga
    14521 ggagaatgca aactcactag aattgaaagg aaaaataaaa tttaaagatt tttatgaatc
    14581 aaaatggctt cctagatatg aactaggtca aactactagg tcaaacaggg ctccaagtaa
    14641 tgtaacaatt agtaatacta aggatatatt ccgattgcac atccttccaa tgtttggtga
    14701 atatgcaatg aattatctga attcaaatac tgagataata tcagatgaat taacaaagaa
    14761 atcgaaagaa tacgcaaata taaaaattat caaaggctat gttcgctcta tgtttgatat
    14821 tgccgaaata ctatgttata tcgaattcaa ccgaactacc aaaattattc aatgtattac
    14881 cgcgcctaag aaaatggctt tagaagaaaa aaggataagg gaagggaatc aagcattatc
    14941 atcagaagag ctgactgatt ggatagaagc agtaaacaat gacttaaata atcacttgct
    15001 tacacttcac gattacacac ttttcatgct taccttatat ctaggtgata gaaaaagtga
    15061 gacttatgct ctgcaatgga aatatattga ttttaataaa aatacggttc gtctcaaata
    15121 tgctttagat aaatatcaaa gaaagacaca tacaaaaggt tggaaagaca ccgttattca
    15181 ggcaccagaa gttgtgatga cattattaag agaatggaaa tcagctcagg ctgaacaatt
    15241 gttgaaacta aaaattatac aaacacctga ccagtacctc tttacctatt ccaaaccttc
    15301 tggagaagta aattgtcctg ttcacgctga ctacttaaac tatcgaatca atgcaataaa
    15361 aaaaaggcat cctgaattgg ctcaattatc tccacacaaa cttcgtcata cctatgcaac
    15421 tatagctagg gaaggtggag caaatatgaa tcaaatctct aatgctttaa cgcactcgga
    15481 tatttctact acaaaaatat acgtgaacac acctgacgta gttgataaat ctgtttttga
    15541 agcatttcaa aaaggattga ataagtgtga ttaacatgtg ttctcaaatg tgttcttatt
    15601 ataaaaaaag acactttcca aataattgaa aagtgtcctg aaatattgat ataaaaagaa
    15661 tattaacgtt ttgagaattg tgaagcttta cgagcttttt taagacctgg ttttttacgt
    15721 tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca
    15781 ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg
    15841 tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg
    15901 aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt
    15961 ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta
    16021 cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag
    16081 tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat
    16141 acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca
    16201 gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc
    16261 aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt
    16321 aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg
    16381 aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat
    16441 ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata
    16501 tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga
    16561 gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt
    16621 caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt
    16681 cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa
    16741 gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca
    16801 agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga
    16861 gctgaacggc aggcttacaa cctcttggta aacggtgttc aatcagctga ccctcggtat
    16921 tagctcaaaa aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag
    16981 gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt
    17041 cgatctctgg cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg
    17101 ttttgaattc gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga
    17161 ttcctttttg gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt
    17221 ctggaatagt acgatcccag cggccaattt catcagggat ttttgtgtta tctgcaaatt
    17281 cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta
    17341 actgagcgtt caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg
    17401 gtcctctcat gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa
    17461 ctaattgatt taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt
    17521 ttcttacact taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg
    17581 agaacgattt tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa
    17641 ttcttctcgc agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac
    17701 aaataatgga tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc
    17761 tttcaattct atcaattcct gtgcttcact gatactttgg atctttccga tgcttctcca
    17821 gcctaaaatc acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta
    17881 acccagtaca tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc
    17941 acggatctct gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag
    18001 acagatccct tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc
    18061 gataaaactg ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg
    18121 atgaaaaatc agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc
    18181 atatggattt tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc
    18241 tgcccaatca aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg
    18301 atccagaaca taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa
    18361 acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa
    18421 ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa
    18481 acctagttct gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac
    18541 ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa
    18601 ttcatggata tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc
    18661 aaacaatcta taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa
    18721 ttcaggttgg ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg
    18781 cgtcccagtc tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc
    18841 gtccgctgct cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc
    18901 caacaatatc aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga
    18961 aggcaactgt ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg
    19021 atcttgttct gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc
    19081 tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa
    19141 tccgattgaa acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta
    19201 agcttgcgta tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg
    19261 ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat
    19321 tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat
    19381 tccgattctc tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc
    19441 cttctactat ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt
    19501 ttcttccgca agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt
    19561 gatggattta ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt
    19621 atggattttt tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat
    19681 cgtaggcggt gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt
    19741 gatgctttct tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc
    19801 tagtctgttt ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga
    19861 aaagctgcct ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta
    19921 ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc
    19981 aaatccttgt gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca
    20041 atcagctgac cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt
    20101 tccataagta gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc
    20161 tgcacgtcca gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc
    20221 ctgatggtaa tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg
    20281 cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc
    20341 ttcttcatca ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc
    20401 taatgcgtca gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat
    20461 aacttctgga tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac
    20521 ttgttcataa gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat
    20581 ccctggctgc tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc
    20641 tttttttgtc atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga
    20701 taacgttttg cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat
    20761 aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca
    20821 gaaggaagat ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct
    20881 tgacaaccag cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc
    20941 tgtgctcctt tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgccgtca
    21001 ataaggacat caaaataact tgtcaaattc gttttttcta aaatgaggcg tgcatttttg
    21061 cttgctgatc ccaatccgat cttgatgttt gtttttttca agtactcaag aacagctatt
    21121 gcaccaggca aaacggatgt ttcatccatt ttttgaatcg cttgaagata tagattgttt
    21181 ttttgctcag caaaggctga tttctctttt tctgagaatt tctgttccat ttttccaaat
    21241 gacagcaatc gctctagtga ctcggttctg gatattcctt tcagttgctc gttttctttc
    21301 tcagtgaatg gaatggacag ctggttagct aaaaccagcc acgcttcgta atgataacga
    21361 gctgtgtcta caagaacgcc atctaaatcg aatagtgctg ctttgatttt catcgattac
    21421 ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat
    21481 tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa
    21541 cggccgaaat gccaacatct gctgcttttt cgcttccttt accaacactt ccgcaaatag
    21601 ctatcacagg aacgccctct ggtactcttc tagcaatccc aactggcgct tttcctttta
    21661 gactttgtcc atctagacgt ccttcgccta cgattacgag gtctgcttgc tgtacccgtt
    21721 tatcgaactc cagtaaatcc aatacgtagt cgatgccaga gactattttc cctgaagcaa
    21781 atgctaacag tcctgcaccc attccaccac cagcactact gcccgatgcc tcgattactt
    21841 gaggggcaaa tctcatataa aaatcatgca tggcttggtc agccactgct aattcatttt
    21901 ctttcaagcc tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat
    21961 tagtaacatc agtaatgaca gtgattttgg ctttaatcag ctcaataggc acgttcgttt
    22021 tatcaactgt agcaatttta cctagatttt ctcccagtgc ttctactttt tgattatctt
    22081 gatcgtaaaa agcataacct agaccttctg ccatgccgat gccgccatcg ttggtactac
    22141 ttccgccaac gccgatgatg atgtgtcgaa caccttgttg caacaattgt cgtatcagtt
    22201 ctcctactcc tcgcgtggat aacctaagtg gctgttgttg gtttagtggg acatctttca
    22261 atccgacgat ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg tactggattt
    22321 ctttgttttt gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt
    22381 ctgctaatga tgaaaccgtt ccttctcctc catcgcctat aggtagcaga tcatattcag
    22441 cctgaacaaa aacttcttga aaacctgatt gaatggcttg tgctgcctct aaggctgtca
    22501 gactttcttt gaaagaatct ggcgcaatca gaatacgcat tattttcccc tccgttccaa
    22561 gatcagttct ttttccagct ggtattggtc tccattgaca aaaataggta atgtttcttt
    22621 cgtctctttt tttattacta tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt
    22681 ccaaataaaa ggaaaagaaa ttttttccca tgctgaagga agtcttggag aaatagaaag
    22741 agttccattc gttactgtaa tacctgaaaa accaaatata actgctagcc atagtgctcc
    22801 taatgaagca gcatgaattc cgtcatctga tgaatgcgga tctggtccta aatcaatcag
    22861 gcacgcttct tggaataaat gatacgccca ttccatttct tggatacggc aagcctctat
    22921 ggcgtgaatc gctttactca acgatgagtc gtggattgtt ttgttttcat agtaatgcaa
    22981 attcttagaa acgattggtt gagaaaaata ctcaggtaat aagtataaca gcatgacaag
    23041 atctgcttgc ttgctgactt ggagttcatt tacttcgcta cgggcataat caagtaaaat
    23101 agactggctg ccaggacgtt ctttgtaagt ggtcaaatca ataactggct tactgagata
    23161 cgtgtcatct tgcggaatca gctgttcttt gttaggcact ggcaaatata acttatctaa
    23221 aaaattttga cattctttca aaaaaagcgg atcgccagct tgatatttaa cagctaaggc
    23281 cacattataa tgcgccagat agttcgtata tgcattattg tcgatatatt ctgtgtattc
    23341 atctggacca atgactctgt gtatctctaa atgatcatcg acttttacag ctctgctcag
    23401 ccaaaaagct gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa acgattgatc
    23461 ctctgttgct tggacatact cttgaacggc ataagcaagg tctgcactga tatgatgttc
    23521 tgcggcagct gaagcaactt cttgtcttgt tccggttttg atattcaatg cagcaaattt
    23581 gggtgtctct tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg
    23641 ataatctctt gctttttgcc gtgcgccttc taatcgatca taacggtaca gcagcaaatt
    23701 ctttgctact tcaggaaact gatataaaaa gaaaggcagg ataaaaattt ccgtatccca
    23761 gaaaacatgc cctttataac cttcgccagt tagtcccttc gcccctacac taacacttgg
    23821 atcattgcgg ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc
    23881 gaaagaagaa tcgccttgga tcgttatccg atgctgatgc caaaaatctg tccaataaga
    23941 catcgactgc tctaaaattt ctgtataact aacgcttttc tgggcttggg caatactttt
    24001 cttaaacggg tctgtttgct gaagagaggt agagagcacg atgatcttct ccagcgtaaa
    24061 ggacgtattt tttctgatct tttctgaaaa ggaagcggtg atccggcgat tttttgcttg
    24121 gaatacacct gctttattga atttagctgc aatcccaata tcaattttac tttctgttgt
    24181 ctggtaggat gcactgatca aatcgtcacc aaatactcgc agttcttttt caaccaactg
    24241 ctgtgtacca aaattggttt gctgtccatc gatacccgtt ttcacatcta cttggcagtc
    24301 ttcggtcaac ggcttgatga ccactttcag ggctgcctga tggagattat tttgatcagc
    24361 aacacgacaa gtaatgactt ccactttttt tccttgagca gtttcaatga gcgcatagcg
    24421 tttaagctct cccatttcta aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc
    24481 aagagaaaaa tgttcaccgt cgatttttat ttcccatctg atgacatctg ggagattcac
    24541 taactcggct gattctgctc ctaaaggtcg gttatataca ccagctgcaa acattcccct
    24601 tacttgattc cgatagtctt cctcctggct tcctctgata ccaaaaaatc catttccaat
    24661 ggtcatctga ctcgcatact tattgatata ctctaaatca aactgttttt cttctacttt
    24721 cataaggtaa taacttctcc tgtttctgcc gaacgatata aattttccac cagattctgg
    24781 atgacatatc cttgccagcc atctgcaatc atctgagtct ggtcaccgag cgcccgattc
    24841 acaaaatgag ccatacttag ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa
    24901 accagctttc catctacatc ttcgaatagt tcgcctggat acagattagc acctgcctga
    24961 tcgccacaaa aagccacatt cagtttgtct tctctgatat tcaaagcaaa cgaagtattc
    25021 agttggatga tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc ttctactgtg
    25081 aatttgcttg gatcccattt cccgaatttc cctgaatttt ttttcgtacc aattttttgg
    25141 aagctgtacg cccgaacgct ttttacttta ggaaatccag taatatacat cgctgtatca
    25201 agcatgtgaa tgccataatc gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac
    25261 gttccccagc ctggaacacc agaccgacga agcgcattcg cttctacgta ataaatatcc
    25321 ccgagaacat caagatgatt cttgatcatt gttgcttcgt cagaaaaacg atgctggaat
    25381 ccataagcca aaatctgccc gtttttatcg gctgtctctg ccatctttct tgcttgatca
    25441 gctgtcatag ctggtggttt ctcgcacatc actgaagcat tagcttcgag ggcagccaat
    25501 acatgttcga agtgaaagcg attgggtgta cagacactca cgatatcggg agaaatctca
    25561 ttcagcattt gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct
    25621 tctgctcgct ggctgactgg atcaaccaca gcggccaatt ctaagacatc tgatcttttt
    25681 tgataataag cagcatgtac tttttctgct acttgtccag cgcctatgat tgctactttc
    25741 ttcatttatt tctctctcct ttgcttattt acttattgtt attctttctc tattctttct
    25801 ctaaatcatt taaatgacgc gctgtttcca gacagttttt caagtattgc aatgattcct
    25861 gataaatttt cacttcattt tctcctcgca aacgacattc atataccata taaccttcat
    25921 actcattttg aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt
    25981 ggtagcgatg attatctgct aaatggatat gacccacata ctctttttgc gtagtcaatg
    26041 cttttgaaat atcatcttct tcaatattca tatgataaaa atcagcgatg atctgcacat
    26101 gtttcaattg attagagtca atgtaatgac gcgcatccgc taacaaattg atcatatggt
    26161 cttggtatcg gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg gctactttat
    26221 ctaaataacg aagtgattcg ctgactgctt tatagtctcc ttctttgctt cggggtgaaa
    26281 ccatcggcgg taatcgataa gtgaacattc cccaagctgc tgggataacg attcctttgc
    26341 ctccaacctc gcttagtgca cgcaaaatct cagcaatttg acgtacacca ttaagacgcc
    26401 gttcttcgat aaagtcaccg atccacccat cgtagccgtt acaagctgtc actactggga
    26461 tccctgtttc tttgattgcc tgtttgactg tgtcgatatt ttctattaac aacttaccgt
    26521 cgatttcgta ttcttcaaat ccacagtctt tgataaatct aaatttttct aaaatgtctc
    26581 ttgggaaaaa tgcttgatct tgcgtaccga atttcattgt tctcctcctc ggacttgaac
    26641 gcccattttg atactttctt ctgggtgctg gtctacataa cgcatgtaag cttctgcgct
    26701 ttctgcaaat ggcacaactg gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa
    26761 taattcccaa catgtgtcct cgattcgttt acggttccat cttgggtaat ctgggtttgg
    26821 ttcactagat gctcgagcaa agacgatttt tgcattattg aaatgtgctt cgcgaccaag
    26881 attaaaccca gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc
    26941 gatccctatt aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa taactgaatc
    27001 tactccacgt ttatctgtca attcctttag acgaaaaccg acatctgctt tcataggatc
    27061 tagcgtgtaa tcggcaccgt gtttttctgc taattctcta cgctgcgcta tgggatcgat
    27121 cccaacgatg gtagtagcac ctgctttttt agctaattga atcgcaattt gcccgatagc
    27181 gcctaaaccg aagattgcta cagcatcacc tgctcgaaca tgtgcttccc ggataccacc
    27241 taaagcgaat tgagcaggat cgtaacatac tgcatttttc cattgatcca accgatcaag
    27301 tttacgtagt ttataattat caacaccgtt tgtaatcagc gtttcacgaa tgggtccata
    27361 agtgcagact aactctcctt tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt
    27421 accgacaatc atatttccta attgaaattc gccaaatacc acaccgcgtt ggcttccttc
    27481 ttctcttggc aagaataggc gccattcttc tgaaaattct tcatcgataa atggacttaa
    27541 tcctctaaag tcgacaattt ctgttcctgg ttttggagta gcgaaaatta ctcggatacg
    27601 tacttcgttc ttttcgatct cacggtcttc gtaatctttg aatccggcta ttcttggttc
    27661 taatgcaact aattttttca tcttttcttt cctctttctt ttctttttat ccttttacgc
    27721 cgccttcgac taaatcacct ttaataaatc gttcagataa cgcatacata atgatgactg
    27781 gtaaagcggt caccaatgaa gcggccatca ttcgtcccca aatgtagtct ggtgtactga
    27841 ataatgtgtt caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag
    27901 caaataaata atcattccac gaaaccataa agcaataaac gaagacagaa atgattccag
    27961 aaatagccaa tggtataatg atccgaataa tgatctggat ccggctaaga ccatcgatca
    28021 ttgctgcttc ttcaatatca gtcggaatcg tgtcaaaata actttttagc atgaatatcg
    28081 cagtaggaag tgtttgaacg accatcgtga taatcagtgc tgtattggta tcatacaaac
    28141 ctagaccgga aatgatttta aataatggaa caacgatcaa aataccggaa aacatataaa
    28201 cggtgtaaaa gctagcattg attttcgagc gagctttgaa gcgtagtttt gacaaagcgt
    28261 atgccccgct gatcccaatc actactgcag ttacggaggc tatcaaggag atcactaaac
    28321 tatttttgaa atacatgatg aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt
    28381 caaaagtcca ttctttcgga aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct
    28441 tgaatgatgt catcagcatg atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa
    28501 tcgtcgcgta aaataagatt ttcctgctct ttttattttt catttcaagt ccaccttctt
    28561 tcgtgagagc aagacgaccg ctgcaataat gatgaacaat agaatcgata tagctgctgc
    28621 ttttccaaga tcattgaacg caaaggctgt ctgatacaaa taaacgccta agatattgac
    28681 tttattcgtc agtagataga catctgtaaa catatagaac atccagatag atcgtaaaac
    28741 aatcacagtt gctagtactg gtttgattgc cggcagtgtc acgatcttga acttctgcca
    28801 agcatttgca ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat
    28861 agccaaaaat gaaataaacg catacggata gtatctccag atagcaaaca aaacgaccat
    28921 aaagaagcta gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct
    28981 atctactaac agatcattga cgattccata actattattg aacatatatt tccaagcaaa
    29041 aatcaaagaa atagatggcg taacataact taataaaatc agtgaacgca ccgttttcct
    29101 aaatctgaac ttgcggttaa gcaaaatagc tacggctaat cctaaagctg tactgcctgc
    29161 tacaactaag aatgtgtaag caactgttaa acctaaagaa cgataaaatt cactatcact
    29221 taaaatttca agataattgc ttaatccgat aaaaacggcg tctgagttag gattcagcgg
    29281 catatcgaaa aaactgatct gaatattgga aagcattgga atcagcacta aaacagctaa
    29341 taaaagcatg cttggtgcta aaagtcccat ggctaatttt aagtcagaat gtttccttgg
    29401 acgattcatt gaaacctccc cctgataatc tgccaatagc tatctacttt tttcgaacac
    29461 cattggcaga acttcttgtt atttcgtatt tttttgtgcg tcagctaaag cttgatcgac
    29521 atcttcgttt ccgacagtta cttggttcac tgcttttccg atcacacctg agcttgtgat
    29581 atctcccatt ttcgtgaaat tcttttcccc gactagtcca aagacttgta cttcatcaaa
    29641 agcactagca atctcagatg gcagctcgcc aaaagacttg atgacttcat tttctttgta
    29701 accatccagt tcgactactt gtttattcac tggttgagca ccaccaggag acatcaagac
    29761 ccacttttcc atgtttttcg gttgagataa gaaagctgtg aatgcttcag cagcctcttt
    29821 ttgtttctcg tctagaccag aagaaatcgt caatccagat actgtgccat agacagcttg
    29881 atctttttgt gttgggatag caaaaccgat attttctgag ttccccgctt cataaacaga
    29941 aggcaaaatg taagttgaat agattgccat tggtgctgtt ccattcatga aagcatcttt
    30001 gatttctgtc acatcattag agcctggcat cgtgtattgg gataagtttt gataaaacga
    30061 caatgcttct ctcatctttt ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt
    30121 tgctccattt gacaaagcaa attgagagaa cgcttgctca ctcattgttc cttcagcagt
    30181 aggcatagca attccatatt gtttattctc tttatctgtg aaatgttgag caatttttaa
    30241 aatatcatcc caattttctg gttctgaaaa accagcttct gataattttt ctttgtcata
    30301 ccaaatacct tgaacccagc cgctaattgg tgcagcaata tagctttttc catcttctga
    30361 acgaacaagg tttttggctc cgtcataata attatcttct ccagcttcct cgataacgtt
    30421 ttggactgcc gtctgatcaa tcagttcatc tttatccatc actttagcaa aatcctgact
    30481 gacttccatt acttctggta atttaccgga acgagctaac gtaacgattt ttgtattgaa
    30541 cgctgattct tctaccggca cttgcttgat tttgatgtct ggattttctt tttcaaaatc
    30601 agcaaccagt tcattgatca cacttaatcg ttcctgttcg acagaagagt gcatgaattg
    30661 aatcgttgtc tgttgtgatt cgttagagct ttgactttca ccacaagcag taagaagaaa
    30721 tccagtagct agcagacagg tagtaacaat aatttttttc atttttttcc acctcttatt
    30781 ttattatttt cagccacata tattcatacg gctggaatat caactttcgt tcttgactgc
    30841 aatggataat ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa agataacctg
    30901 ctgattgatt ggaaaaatta tggataacta agactttctc ttccttcgct tcgcgaataa
    30961 aactgataaa agaatctgct gagtccagta agtaaatcaa actattcgga tggaaggctt
    31021 tttctttttt tcgaatagat agcatctggg tcaattcacc atatactgct gttcttaatg
    31081 gatctgtctg caactctttc tcaatatctg ctaacaaaaa tttttgccga ttgatttttc
    31141 gattctctcc tgttttttct acgcctttgt aatcatttcg acttcccagc acactttgaa
    31201 tataaattgc cggcacgcct ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt
    31261 tgcttctcca ttccgtcgta tctgttggag aacttaacgc atctagatac gtagcgttta
    31321 tctcatatgg actggtcgtt ccatctgggt ttttcttgta gtttactcgg gcaccctctt
    31381 tcttcagctg ttccactaat tcaaggatcg ttttttccgg cagaatacct cgtaccggat
    31441 tcaatccgat accgtcatgg gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga
    31501 aatcgatggt ttttaaccat tctttcaact tacttgtatt acccgaacga atcgcttgaa
    31561 gggtcaaagg tggtagcgaa aactgataga ccatctgtgc ttcattctcg ccattcccaa
    31621 aataactgat attatctttg tgtggcacat ttgtctcagt gatcaatacc gttcctggag
    31681 ctgcttcatc agcgattgca cggaataatt ggatgatttt atgcgtttct ggcaggtgta
    31741 tacaagaagt tcctagttct ttccataaaa atcccacagc gtccaagcga ataaatgaag
    31801 cgcctttttc taagtaaaac aaaagaatat cgatcatttt caataaaact tctggatttt
    31861 taaaattcaa gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt
    31921 ttttgtcaat gaacgttgta agtaacgggc tggtccttgg tctagtcacg ctgcttgtat
    31981 ctgtatccag tggcaaatca ataaaaaaat ctttgtacgt atcattcaag tttaaatatt
    32041 cctgaaacca agtactttcg gcagacatat gattgcagac aaagtcgaac attaaccgtg
    32101 tttcgcttct catttcttct atatcttccc atgtgcccaa acggtcgttg attgcctgat
    32161 aatttacaac ggagaaacca tcatcagatg tatatgggaa aaatggcaag aaatgtacaa
    32221 ctctaaatgt atctgccaaa tacttttgat aaaatttctt aaatgtacgc aaagttggtt
    32281 gctgctcttc aaaaaattga tccccataag taatcaagaa aatatcttct tcgtcccaat
    32341 gcagtttgcc tgcctggcct ttcttctgct gataaacact gactttttgt tcaatcatag
    32401 aggcggtttt ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca
    32461 tcggattccc ctttcttttt tgggagcgtt cccaacgatt tgatcatatt atggaaaacg
//
LOCUS       DAGCWU010000015        31468 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.015, whole genome shotgun sequence.
ACCESSION   DAGCWU010000015 DAGCWU010000000
VERSION     DAGCWU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.015"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            189..3467
                     /locus_tag="MBZ71_000565"
     CDS             189..3467
                     /locus_tag="MBZ71_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBT4428542.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(4008..5915)
                     /locus_tag="MBZ71_000566"
     CDS             complement(4008..5915)
                     /locus_tag="MBZ71_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBT4428543.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6045..6920
                     /locus_tag="MBZ71_000567"
     CDS             6045..6920
                     /locus_tag="MBZ71_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBT4428544.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            7000..7974
                     /locus_tag="MBZ71_000568"
     CDS             7000..7974
                     /locus_tag="MBZ71_000568"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBT4428545.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8154..9299
                     /locus_tag="MBZ71_000569"
     CDS             8154..9299
                     /locus_tag="MBZ71_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBT4428546.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9436..9780
                     /locus_tag="MBZ71_000570"
     CDS             9436..9780
                     /locus_tag="MBZ71_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBT4428547.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10051..11337
                     /gene="efmA"
                     /locus_tag="MBZ71_000571"
     CDS             10051..11337
                     /gene="efmA"
                     /locus_tag="MBZ71_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBT4428548.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11475..12074)
                     /locus_tag="MBZ71_000572"
     CDS             complement(11475..12074)
                     /locus_tag="MBZ71_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBT4428549.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12478..13599
                     /gene="mnmA"
                     /locus_tag="MBZ71_000573"
     CDS             12478..13599
                     /gene="mnmA"
                     /locus_tag="MBZ71_000573"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBT4428550.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13845..15266
                     /gene="arcD"
                     /locus_tag="MBZ71_000574"
     CDS             13845..15266
                     /gene="arcD"
                     /locus_tag="MBZ71_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBT4428551.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15280..16398
                     /locus_tag="MBZ71_000575"
     CDS             15280..16398
                     /locus_tag="MBZ71_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBT4428552.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16458..17750)
                     /locus_tag="MBZ71_000576"
                     /pseudo
     CDS             complement(16458..17750)
                     /locus_tag="MBZ71_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
     gene            17882..18460
                     /locus_tag="MBZ71_000577"
     CDS             17882..18460
                     /locus_tag="MBZ71_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4428553.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18589..18966
                     /locus_tag="MBZ71_000578"
     CDS             18589..18966
                     /locus_tag="MBZ71_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBT4428554.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18899..20971
                     /locus_tag="MBZ71_000579"
     CDS             18899..20971
                     /locus_tag="MBZ71_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBT4428555.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21182..22129)
                     /locus_tag="MBZ71_000580"
     CDS             complement(21182..22129)
                     /locus_tag="MBZ71_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4428556.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22375..22839
                     /locus_tag="MBZ71_000581"
     CDS             22375..22839
                     /locus_tag="MBZ71_000581"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBT4428557.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22901..23944)
                     /locus_tag="MBZ71_000582"
     CDS             complement(22901..23944)
                     /locus_tag="MBZ71_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBT4428558.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24321..25286)
                     /locus_tag="MBZ71_000583"
     CDS             complement(24321..25286)
                     /locus_tag="MBZ71_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBT4428559.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25520..26365
                     /locus_tag="MBZ71_000584"
     CDS             25520..26365
                     /locus_tag="MBZ71_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4428560.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26556..27743
                     /locus_tag="MBZ71_000585"
     CDS             26556..27743
                     /locus_tag="MBZ71_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBT4428561.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27749..28105
                     /locus_tag="MBZ71_000586"
     CDS             27749..28105
                     /locus_tag="MBZ71_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBT4428562.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28107..28445
                     /locus_tag="MBZ71_000587"
     CDS             28107..28445
                     /locus_tag="MBZ71_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBT4428563.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28521..28777
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28840..29874
                     /locus_tag="MBZ71_000588"
     CDS             28840..29874
                     /locus_tag="MBZ71_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4428564.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29867..30553
                     /locus_tag="MBZ71_000589"
     CDS             29867..30553
                     /locus_tag="MBZ71_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428565.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30577..31425
                     /locus_tag="MBZ71_000590"
     CDS             30577..31425
                     /locus_tag="MBZ71_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBT4428566.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT        10270 a         5728 c         6670 g         8800 t
ORIGIN      
        1 ttttatacgt tattttaaaa aaacttattc tttttccgtt aataaatctc tttattatca
       61 acaacttaag ttatattttt tctttttata aaatacactt attataattt tgttttttat
      121 tgacagtaaa aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag
      181 gaatacactt gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc
      241 tcagtacccc ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga
      301 acgaagaaaa tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa
      361 ctagtgaaga tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg
      421 aaaagacaaa aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag
      481 aaagcacaag tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt
      541 ccacgaataa cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc
      601 aagcaatccc caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga
      661 cctatacgaa tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc
      721 atttgtttgg gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat
      781 atgtctggga ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc
      841 gacttggatt ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc
      901 cagctcctgg gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg
      961 aaaaagaaac aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca
     1021 cgaactcgat tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat
     1081 attattacga tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa
     1141 aattatacgc ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga
     1201 cgatcaaggt agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc
     1261 aagaaagacc tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag
     1321 gaaaccaaga tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa
     1381 ataaagcaac aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg
     1441 aatttgatta tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc
     1501 cagataaaaa ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga
     1561 ttcttcatac agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag
     1621 ggtcagatgc aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat
     1681 acgcatatgc tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat
     1741 tgacgattcc aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga
     1801 aaaatccaga aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac
     1861 tgttcagtca ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa
     1921 aaggattaca gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg
     1981 gaaatacagg aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc
     2041 ataacttagt caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg
     2101 atggatttgc ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt
     2161 atcataatgc agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa
     2221 aagtaatcat caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag
     2281 aaggtaacgg taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga
     2341 tcaatagttt gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata
     2401 ttatcgtcaa tagcagtaca agttttaata acggttcata taatgtggct ctttatacct
     2461 cttcagcagg aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc
     2521 ttgagatgag agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt
     2581 tgaactacta ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat
     2641 caaaagattg gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc
     2701 gctatgaaaa tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc
     2761 aaacagaaaa ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag
     2821 aaacctcacc gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa
     2881 aaccaaatga acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg
     2941 cagaaggaaa tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa
     3001 atcaaggaac gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg
     3061 aaacacagtc aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag
     3121 gagaatatac gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg
     3181 ctcatacaga atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc
     3241 ccccgggaaa tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac
     3301 caaatggaaa accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc
     3361 aaggactccc aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt
     3421 tgttaatcgg tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc
     3481 ttttatgatg taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag
     3541 atgtctcact tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct
     3601 gttcttatta cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat
     3661 cagctgaccc tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt
     3721 ttttctctaa tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa
     3781 aagccagttt ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa
     3841 aatttttctt actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc
     3901 agaagtaact tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc
     3961 caaattcctt cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc
     4021 actctttttt ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag
     4081 aattcgtcaa ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg
     4141 atttgcagta aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa
     4201 ttgacaaaga attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag
     4261 cttagaccgc ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg
     4321 tcagcaaaag gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt
     4381 gtaagatctt cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg
     4441 ttattatcaa atgcatggga aatctcatga gcgataacag ctccgattcc accataatta
     4501 gcagagcttg attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg
     4561 atcacattcc gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct
     4621 gaacgatcca ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa
     4681 acaagagtat ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg
     4741 aataatgctg ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg
     4801 acggcttttt cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa
     4861 acggcgatca tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat
     4921 ttttttccgt agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct
     4981 gcttttttct gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg
     5041 acttggcgga attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc
     5101 cagcttttga ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac
     5161 actggatcag ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt
     5221 ttcaagtcca gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt
     5281 ttgctgtaat ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat
     5341 tgcactgctt gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat
     5401 aactgaacag ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat
     5461 gcataatacg tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt
     5521 tgcgcattct tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac
     5581 agccattctg gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg
     5641 attataggtt gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt
     5701 tgataatatg caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc
     5761 agatcgtctg tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg
     5821 cctgtagctg gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct
     5881 tcatagaaat cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc
     5941 gtgtgcccat tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat
     6001 tcgaacaatc aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc
     6061 gatcccaact gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta
     6121 aaaaattagc cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc
     6181 tggtttttga agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt
     6241 ctccagcaaa atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg
     6301 aagagccaat cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg
     6361 aagaaaaaag gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt
     6421 ttttgaggta taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag
     6481 cagttctcct tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc
     6541 gcaagaaaaa tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac
     6601 cactaggact tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga
     6661 agaatggcaa tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa
     6721 aacgtatacg tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat
     6781 atggcaatca ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt
     6841 acaaagtaca cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg
     6901 gaacagtttc acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt
     6961 taaactatcg aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat
     7021 gacacgttga agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa
     7081 gtgttcggaa cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc
     7141 atcaatatcg aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca
     7201 cctgtcaatg attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt
     7261 gcgaaaacga tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg
     7321 aaacctcatg aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca
     7381 actcgagtac tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct
     7441 gtagataatt tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg
     7501 ggagatgaca tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt
     7561 tcagaatatt tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag
     7621 ggtggttatg tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg
     7681 aacacaggag caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa
     7741 gtatatgcct gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat
     7801 gcaccaatca aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca
     7861 gaaaacttaa caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat
     7921 gaaaatacac cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa
     7981 aaaagaggga aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt
     8041 tgtcacagct ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct
     8101 ttggtttgtt cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg
     8161 aagtgaaaac tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg
     8221 aagcgatgac ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg
     8281 gtcgccacgc gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag
     8341 caaaacctca cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct
     8401 tagaaacagc gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac
     8461 atcctgctgt tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc
     8521 tgcctgtaga tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag
     8581 atacgatctt ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca
     8641 aagaaatcgg tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag
     8701 cgtacggaaa ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg
     8761 ctcataaaat caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata
     8821 tccctgcatt gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt
     8881 tagcagggat ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag
     8941 cacgtaacaa caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg
     9001 aaatccctta tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc
     9061 gcttaattgg cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta
     9121 tttctactgg ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa
     9181 tgtatggaga agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa
     9241 ataccgaaga cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac
     9301 tagtgaagtc attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac
     9361 ttaactgaaa aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt
     9421 atcgaggtga aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc
     9481 tatcgtttgc atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca
     9541 aacggaggca attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga
     9601 ttcaaattaa aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca
     9661 gcaaatggat tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat
     9721 aaattttatt ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga
     9781 tctgtggata taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg
     9841 taaggacgac gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt
     9901 taatattttg ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt
     9961 tagggaaaaa ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta
    10021 tacaaaatga aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg
    10081 aattggaaga gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt
    10141 atggtcgtcc aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt
    10201 ttgagttttg ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga
    10261 ccgctagtgg atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg
    10321 attttcgcgc ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta
    10381 gtttttgtgt ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag
    10441 tcgattatgc cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga
    10501 atggtccaat cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc
    10561 gttcctgtca attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta
    10621 ttgatttttg tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt
    10681 tttaaagatg caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata
    10741 acgatcaatg gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg
    10801 atgacgctag attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac
    10861 gcagtcggaa tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga
    10921 atgaaaccta ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg
    10981 gttccaaacg atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt
    11041 gcaacgccat atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat
    11101 gttttaggtc gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga
    11161 ttattatttg caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc
    11221 ggaatcggga ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat
    11281 gataaagaat tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat
    11341 ggaatggagt tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg
    11401 gcaataagcc tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat
    11461 atagggcagt tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt
    11521 tgttgctcag ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt
    11581 gcgtctacgc ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca
    11641 tgaagattgc ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat
    11701 tgttgatggt cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg
    11761 tcagtaagcc taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc
    11821 cctttgatca agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt
    11881 gctttttcat attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct
    11941 tttattactg cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag
    12001 agccgcggag caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg
    12061 ataaagtaat tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat
    12121 ctgagcaact cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg
    12181 ataagaagca attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga
    12241 aaggctggcc ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg
    12301 attttttggt tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac
    12361 gacttgataa ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa
    12421 agttgcgacc ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg
    12481 acagacaaca gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta
    12541 acagcacttt tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg
    12601 gatgacacgg acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa
    12661 gtagctgctc aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat
    12721 cgtgtatttg aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg
    12781 atgtgcaaca aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc
    12841 gattatgtag ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac
    12901 atgcttcgtg gagtagacaa taataaagat caaacttact tccttagcca attatcacaa
    12961 gaacagcttt caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca
    13021 attgctgaac gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt
    13081 attggtgaaa agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat
    13141 atggtcactc ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt
    13201 ggtcaacgtc aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt
    13261 gtaggaaaag atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca
    13321 ttgtatgcca caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca
    13381 aaagaattca agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt
    13441 cgcttattgg atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact
    13501 cctggacaag cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac
    13561 cacgcatatc aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa
    13621 cagctatggc taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa
    13681 taaaattaat ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc
    13741 aataatatga ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc
    13801 ttttgcaagt gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga
    13861 cgggtattag caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg
    13921 tgtttgggat cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat
    13981 ggataatcgt tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa
    14041 agcgtccaga tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag
    14101 gtggcttcat cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg
    14161 caacgatgct gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg
    14221 ttccttctat tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg
    14281 gagttgaagg agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat
    14341 tcctattttt aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt
    14401 ggggtaacgt atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc
    14461 agattaaagg ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg
    14521 ttttagccaa ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc
    14581 tttgtttatt gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag
    14641 aagagttagc aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa
    14701 attggggagc agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt
    14761 cttggacgat gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga
    14821 aaggttgggg aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa
    14881 tcttgactaa tctattctta ttgactttct tagttactga ttatgcctat caatttgcct
    14941 attctttatg tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc
    15001 agttttcttt aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt
    15061 ttgaattgat gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct
    15121 atcttcctgg tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg
    15181 gaaaagaaaa agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc
    15241 ttgctgtagg ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt
    15301 gtagaagaat ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg
    15361 atcgaagcat tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt
    15421 ttcgaaaaac gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc
    15481 aaacaagaag tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat
    15541 ggagcgactg gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt
    15601 gtttcgatgc ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct
    15661 gttgactttg ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa
    15721 gccttagtca aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc
    15781 acattgctag atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca
    15841 tatgtattga tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc
    15901 gtagatgtct atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca
    15961 ggtattcgta ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat
    16021 cgagactaca cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg
    16081 aagaataaag aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt
    16141 ttaaaacaat gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct
    16201 acttctttca ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta
    16261 gaagaaacag gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga
    16321 ctaggttact gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc
    16381 ttgaaaaaac aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca
    16441 gtgtttttca tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact
    16501 cctgaagctt tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca
    16561 agcaagaagt tgaccaaaac aagaagagaa gtcgaaagaa agacgccgcg ataaccaaat
    16621 gcactggaaa cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat
    16681 tggttataac tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct
    16741 tggacagcag ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat
    16801 tgccaaacat tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca
    16861 atcgcaagta ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct
    16921 atcaaagtgg caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca
    16981 tgtccatgca gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat
    17041 gcttggatga tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct
    17101 ttgaaaatct gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg
    17161 acgaaaaaca gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta
    17221 gggcgatagc caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct
    17281 gtaacagaac cagttgctaa ggttcaaaga acttgaccac ttttttcttt tggcgtccca
    17341 gttgcgacaa gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc
    17401 aatgcgacta attgatacac actagtcacg caacccatca aagaaatgac gattgccatt
    17461 cctaatgaag cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac
    17521 caaggagaga taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag
    17581 ttcaattgtg acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg
    17641 actaaactaa atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt
    17701 tcccaagttt ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct
    17761 tctttctgaa aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa
    17821 tgaaagtctc ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt
    17881 tatgagagga aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg
    17941 ctttcgagaa ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa
    18001 acgcttggat atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa
    18061 aacagcgcta acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt
    18121 gggaaaaagt ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt
    18181 ttcaaatcaa gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt
    18241 attagatgga atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt
    18301 aacgaaagaa gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct
    18361 ttccttacgt tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta
    18421 tttttggatg atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa
    18481 caattttaag atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg
    18541 aaaagtgact aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta
    18601 attaccatgt tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt
    18661 gaagaagaag aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg
    18721 aacgaaatcc atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt
    18781 tggaatgacg gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag
    18841 ggacttgcgt tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat
    18901 gaaacaacta attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg
    18961 tgctaatcaa aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct
    19021 tggtcattta ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca
    19081 aatggacacg ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg
    19141 acatcaatta aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat
    19201 tgcgacagat gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca
    19261 tttcaataaa ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac
    19321 tggattcaag caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc
    19381 ccgggcaaaa gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta
    19441 cgcggataat ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca
    19501 agaaaagaaa atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg
    19561 aaaagaacaa gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc
    19621 acaaaaactt gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa
    19681 attaaaaacg gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa
    19741 tcaacgctat ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac
    19801 ccataagatc gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag
    19861 tacgatgaaa gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt
    19921 gaaaaacgga gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca
    19981 ccatgcactt ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg
    20041 aaaaatctat caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt
    20101 cagccaaaca aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg
    20161 tgtcgtccaa gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga
    20221 aaaaggaatg agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa
    20281 accattgtca gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc
    20341 tacgcgtgca gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca
    20401 aggactgcaa gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt
    20461 aacaccagat ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa
    20521 acaagcagga accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat
    20581 caaatcaagc aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg
    20641 tggggccaat ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc
    20701 agattgcaac taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg
    20761 tcaccggaaa atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga
    20821 tggaacgaca gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga
    20881 agaacgacgc ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc
    20941 agcagcacta aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt
    21001 ggtcagcttc aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat
    21061 ttcggcttat ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt
    21121 gacaagactt ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat
    21181 attattggtg aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa
    21241 caggacgctg tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac
    21301 ataacaattg gattccacca atgaacagga agatcgagac catggaaggc catcctgatg
    21361 taggatcacc aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga
    21421 cacaagacaa agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga
    21481 taccatcaat cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac
    21541 gttcgcggtt ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt
    21601 ttgaaaatcg gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca
    21661 agcggaaatc acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt
    21721 agaatttttt cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg
    21781 tgccgacaca atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg
    21841 gcggatcttg cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc
    21901 cagcgtacag agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag
    21961 gataagctga ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa
    22021 taaacaaata ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag
    22081 gaacagtttt ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc
    22141 tttcaatcgt atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc
    22201 tttaaaattt gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt
    22261 ttaccatgtt tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag
    22321 ttttggatag agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa
    22381 atacacacac ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta
    22441 tcttttcagc caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa
    22501 gaacagtttg aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata
    22561 aagagaggta cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga
    22621 gaaatccgaa cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg
    22681 attgggcagg caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc
    22741 gggcaaaatg gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa
    22801 cgccaattgt tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat
    22861 tgttccatgc gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa
    22921 taaatagtaa agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc
    22981 gtgtaccttc tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag
    23041 tgatctcaaa ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa
    23101 acaagctgtt gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt
    23161 ttcgtttttt ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga
    23221 atttttcaaa cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca
    23281 tggcgaattc gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag
    23341 attggagtgt accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta
    23401 atccaagaaa agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga
    23461 tctgtccttt ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag
    23521 aaccacaaac agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt
    23581 ttttaccaat caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa
    23641 ttccttttac accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa
    23701 cagagaattc taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt
    23761 taaaaaaagt atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact
    23821 gtggcagata aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac
    23881 cgggtaagat ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt
    23941 tcatttgatc ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact
    24001 aaaccagtat tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt
    24061 ttcgaataaa cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa
    24121 tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg
    24181 acctcttggt aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa
    24241 aaacatgaac agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc
    24301 acaacctatt gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta
    24361 tgttctttaa ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca
    24421 aacagacatt ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg
    24481 aagtaatgaa cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct
    24541 gcatagcttg gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa
    24601 tacaatactt gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag
    24661 tttttaagat aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga
    24721 cgtgtcggat gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag
    24781 aacgcatagc agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc
    24841 ccggcagtta cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt
    24901 gcttcaattg aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc
    24961 acttggaaga taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat
    25021 gaaacaaaat aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg
    25081 atgattggat catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa
    25141 atgaatatca taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga
    25201 tagtctttaa acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc
    25261 ggaataattt tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta
    25321 gtaccaagat gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag
    25381 acagctgatt gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt
    25441 ctattataga tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa
    25501 aagttatgga agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac
    25561 aagaaatttt tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca
    25621 aatcaaacag ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga
    25681 gatgcttcca actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga
    25741 atgggaagaa actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat
    25801 cgttgcttcc cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa
    25861 aaggtttcct aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca
    25921 agtagcgaaa catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt
    25981 acaaagatac tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt
    26041 gtggcttgta agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga
    26101 gatgaatatc caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct
    26161 gaaggaagga atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat
    26221 gactccgttg aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga
    26281 tcagctgcta gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca
    26341 cagcgaggag aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca
    26401 ctaaagataa gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag
    26461 ttaatttttc ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata
    26521 atcagcagta tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga
    26581 acgataatcg tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc
    26641 tgctctcatt atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg
    26701 tagggatgca agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt
    26761 tgaattccaa atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa
    26821 tgggactctt gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact
    26881 ggtttcgatt cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct
    26941 tgatgatggc actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat
    27001 cggacttttg gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca
    27061 tggcccaaga tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc
    27121 tgatgtctgg aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg
    27181 gagcaggaac gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg
    27241 gattcaaatc ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt
    27301 caggaatgag ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg
    27361 gtaaaggatt caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta
    27421 ccgttatcgg tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta
    27481 ttttaatcta tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata
    27541 tcgcaagcgg tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata
    27601 tcggattact tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga
    27661 ttttagggaa catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac
    27721 ctgcaagatc ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc
    27781 aacatgcaaa aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga
    27841 tatgatcaaa gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt
    27901 accaatccgt cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga
    27961 tcaagtaggc gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct
    28021 gatcaagcga ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa
    28081 aatttatgaa ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc
    28141 tttggatctt atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag
    28201 aacttggaga catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg
    28261 aacctctgat cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg
    28321 gtacagtttc ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag
    28381 atgaaatgaa tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat
    28441 tataaaatca gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca
    28501 taagcaatcc tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt
    28561 gaagagagcc ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag
    28621 cagagtaagc gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata
    28681 cggaaagaag ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca
    28741 aggtggtaac acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct
    28801 tttttgtttg aaaaattata agagaaagat gtgaatggca tgtcattaat ccaactaacg
    28861 aatgtcaaaa agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca
    28921 ctaaacgttg aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt
    28981 acattagtcc gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac
    29041 gatcaagata ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt
    29101 atgatcttcc agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg
    29161 ccgctggaat tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg
    29221 cgtttagtcg ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag
    29281 aaacaacgcg tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat
    29341 gaggctacta gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg
    29401 atcaataaga aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc
    29461 aaaatctgca acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta
    29521 ttgactgtct tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc
    29581 gaaccagaag ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc
    29641 cgtttggcaa ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg
    29701 attcgacgct ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa
    29761 ggttcctttg gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca
    29821 gaagtgttca tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt
    29881 ttcagaaaca tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct
    29941 tgatacacta tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt
    30001 gggattgatc ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg
    30061 aatcgtatcg atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt
    30121 gatcattcca tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc
    30181 agctcttgtt ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga
    30241 agtcagccct ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat
    30301 ctggaaggtc ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac
    30361 gatctcgatg atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg
    30421 tcttgcttgg caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt
    30481 aattatttta ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac
    30541 agacaaaaga taataggata tagggggatg taggatatga aaaagaaaat ttttggattt
    30601 gcagcagcgc ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa
    30661 gattcagcaa ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac
    30721 gcagagattt tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc
    30781 gtcaaattcg atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc
    30841 aattacttcc agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt
    30901 gtcaatgccg gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat
    30961 gtttccgaat taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga
    31021 cgcatcatca caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg
    31081 gaaacagcaa cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg
    31141 atcgacccat cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc
    31201 aacgcaaact tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa
    31261 aaggacaact ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac
    31321 aaaatcaaaa aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac
    31381 aaatggggcg gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa
    31441 agatgagaaa taagtctgta atggctta
//
LOCUS       DAGCWU010000016        31388 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.016, whole genome shotgun sequence.
ACCESSION   DAGCWU010000016 DAGCWU010000000
VERSION     DAGCWU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.016"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            1..369
                     /locus_tag="MBZ71_000591"
     CDS             1..369
                     /locus_tag="MBZ71_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBT4428567.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="MBZ71_000592"
     CDS             549..1490
                     /locus_tag="MBZ71_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBT4428568.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="MBZ71_000593"
     CDS             1559..2290
                     /locus_tag="MBZ71_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBT4428569.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="MBZ71_000594"
     CDS             2434..4614
                     /locus_tag="MBZ71_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBT4428570.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="MBZ71_000595"
     CDS             4634..5116
                     /locus_tag="MBZ71_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBT4428571.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="MBZ71_000596"
     CDS             5132..5893
                     /locus_tag="MBZ71_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBT4428572.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="MBZ71_000597"
     CDS             6024..7358
                     /locus_tag="MBZ71_000597"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBT4428573.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="MBZ71_000598"
     CDS             7665..8150
                     /locus_tag="MBZ71_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBT4428574.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="MBZ71_000599"
     CDS             8143..8826
                     /locus_tag="MBZ71_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBT4428575.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="MBZ71_000600"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="MBZ71_000600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBT4428576.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="MBZ71_000601"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="MBZ71_000601"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBT4428577.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="MBZ71_000602"
     CDS             12442..13071
                     /locus_tag="MBZ71_000602"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBT4428578.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="MBZ71_000603"
     CDS             13123..13455
                     /locus_tag="MBZ71_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBT4428579.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="MBZ71_000604"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="MBZ71_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBT4428580.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="MBZ71_000605"
     CDS             14281..15654
                     /locus_tag="MBZ71_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBT4428581.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="MBZ71_000606"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="MBZ71_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBT4428582.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="MBZ71_000607"
     CDS             16709..17149
                     /locus_tag="MBZ71_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBT4428583.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="MBZ71_000608"
     CDS             17293..17994
                     /locus_tag="MBZ71_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBT4428584.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="MBZ71_000609"
     CDS             17991..19112
                     /locus_tag="MBZ71_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBT4428585.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="MBZ71_000610"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="MBZ71_000610"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBT4428586.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="MBZ71_000611"
     CDS             20491..21399
                     /locus_tag="MBZ71_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBT4428587.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="MBZ71_000612"
     CDS             21434..21706
                     /locus_tag="MBZ71_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBT4428588.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="MBZ71_000613"
     CDS             21717..22763
                     /locus_tag="MBZ71_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBT4428589.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23014..25623
                     /gene="clpB"
                     /locus_tag="MBZ71_000614"
     CDS             23014..25623
                     /gene="clpB"
                     /locus_tag="MBZ71_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBT4428590.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25774..26460)
                     /locus_tag="MBZ71_000615"
     CDS             complement(25774..26460)
                     /locus_tag="MBZ71_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428591.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26438..26932)
                     /locus_tag="MBZ71_000616"
     CDS             complement(26438..26932)
                     /locus_tag="MBZ71_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBT4428592.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26952..28172)
                     /locus_tag="MBZ71_000617"
     CDS             complement(26952..28172)
                     /locus_tag="MBZ71_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4428593.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28319..30154
                     /gene="lepA"
                     /locus_tag="MBZ71_000618"
     CDS             28319..30154
                     /gene="lepA"
                     /locus_tag="MBZ71_000618"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBT4428594.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30248..31375)
                     /locus_tag="MBZ71_000619"
     CDS             complement(30248..31375)
                     /locus_tag="MBZ71_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4428595.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10450 a         5612 c         6724 g         8602 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccggc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag
    22801 aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat atacatacaa
    22861 tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa actatgtgta
    22921 tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag
    22981 tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg
    23041 cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc
    23101 gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg aaatttctat
    23161 acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag
    23221 tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt
    23281 cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg
    23341 gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat
    23401 aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa
    23461 agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat
    23521 ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc
    23581 cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa
    23641 cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat
    23701 gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct
    23761 ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa
    23821 aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga
    23881 aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa
    23941 ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa
    24001 gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc
    24061 agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac
    24121 aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg
    24181 gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct
    24241 atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct
    24301 gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa
    24361 ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa
    24421 gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa
    24481 gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct
    24541 caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg
    24601 gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc
    24661 cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt
    24721 cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt
    24781 tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca
    24841 actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa
    24901 gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta
    24961 gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc
    25021 agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc
    25081 ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta
    25141 gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg
    25201 gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta
    25261 ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca
    25321 ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa
    25381 cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct
    25441 gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa
    25501 gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta
    25561 acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc
    25621 taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc
    25681 cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct
    25741 tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt
    25801 ataactttca aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa
    25861 ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc
    25921 gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc
    25981 gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa
    26041 taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat
    26101 caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca
    26161 aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa
    26221 aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac
    26281 ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg
    26341 agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc
    26401 aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat
    26461 ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca
    26521 atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt
    26581 gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc
    26641 cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga
    26701 tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag
    26761 caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt
    26821 gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt
    26881 tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat
    26941 catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct
    27001 agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca
    27061 cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct
    27121 ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca
    27181 ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca
    27241 aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg
    27301 atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata
    27361 tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt
    27421 cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag
    27481 tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca
    27541 tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca
    27601 tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat
    27661 cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag
    27721 gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga
    27781 tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga
    27841 tcttgcatca atccatggtt agcatattct ggataagttc caactccagt cacaccaccc
    27901 attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc
    27961 agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa
    28021 cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg
    28081 aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat
    28141 tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata
    28201 acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa
    28261 attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat
    28321 ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca
    28381 tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac
    28441 gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat
    28501 cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat
    28561 ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct
    28621 tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac
    28681 attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa
    28741 aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg
    28801 aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat
    28861 tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa
    28921 agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat
    28981 cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt
    29041 tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt
    29101 cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga
    29161 tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa
    29221 tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga
    29281 agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca
    29341 agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca
    29401 agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta
    29461 ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga
    29521 acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa
    29581 tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat
    29641 ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt
    29701 ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga
    29761 aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa
    29821 agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat
    29881 cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc
    29941 tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc
    30001 taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg
    30061 gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt
    30121 tctgaaaatg gacgaggacg aaccgaagaa atagcaaggg tttgtttgaa aacagtgtaa
    30181 aaaataaacc atgtttacat ggatttgcca ccggtttgcc accggtggtt tttttcggtg
    30241 gcaaaaatca agaattcttt ctaagatttt ccatgatatc gaccgtttca tttctcatct
    30301 tatctgttac atgcgagtaa gtatccatcg taattgaaat tctgctgtgg cccaatcgtt
    30361 cagagatttc tttcattttt gcgccatttt cgagaagaag tgtagcatga gtatgtctga
    30421 gagaatggaa gttaaaagag agggagagtg cattcgatat cctcctagta ttccatttca
    30481 ctacacttgg cgtaactagc tcaccgtctt ccttcgtaca tactgcattt gaatcaatgt
    30541 agagctttcc atacttcatt cgattttcta attgttgttt cttatgtttt ttcagaattg
    30601 ctagcaaggt ttgtccaata aaaatcgttc gattggaact gcttgtcttt ggcgtaccat
    30661 atacccatgc gccgtcattc tttaccattt gtttctctac agtaattgtt ccatttgaaa
    30721 aatcgacatc atcccacgtc agaccacaaa cttcgccaac gcgcattccc gtataaaatc
    30781 caatattcaa aggaatatag aaaggatgtc cttcaggagt aatttctagc atatgatcaa
    30841 agtcctcaag agaaatgatt tttagatctt ttttagtcgt tggtcgtgct tcatattttg
    30901 gtatttttac atacagcata ggattttgct tgattaatcc ccaaggataa accgccatat
    30961 tcagcgcatt cttaaggaca gagtgagtaa tagtcattgt tttcttcgag taaccctttt
    31021 taaattcagc attgatgaaa ttttgtaaaa gagcagggga gagatccgta agttttttct
    31081 ttcctaaata accgtttata tgatttttga tggtaaatcg gtagttttca taggtattgt
    31141 attttagatt tagtttaacg tattcctcca tccaaaaatc aaggtattgt tttactcgag
    31201 tatccgtacc taaaaagtat tgtcctgttt cgtcaatatc tgataaaact tttcgtaaag
    31261 cagcttcggc ctctggtcgg gtgtctccgc caactttctc cacttttttt cttgagccat
    31321 catcattgat atcttcgccg tttgtataat taagcgagac aaataaaaaa gccatttatg
    31381 ttacactc
//
LOCUS       DAGCWU010000017        29026 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.017, whole genome shotgun sequence.
ACCESSION   DAGCWU010000017 DAGCWU010000000
VERSION     DAGCWU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.017"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1030)
                     /locus_tag="MBZ71_000620"
     CDS             complement(<1..1030)
                     /locus_tag="MBZ71_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4428596.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="MBZ71_000621"
     CDS             complement(1112..1801)
                     /locus_tag="MBZ71_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBT4428597.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="MBZ71_000622"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="MBZ71_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBT4428598.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="MBZ71_000623"
     CDS             complement(2328..2831)
                     /locus_tag="MBZ71_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBT4428599.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(2951..3078)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3157..3846)
                     /gene="rplA"
                     /locus_tag="MBZ71_000624"
     CDS             complement(3157..3846)
                     /gene="rplA"
                     /locus_tag="MBZ71_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBT4428600.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(3950..4372)
                     /gene="rplK"
                     /locus_tag="MBZ71_000625"
     CDS             complement(3950..4372)
                     /gene="rplK"
                     /locus_tag="MBZ71_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBT4428601.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4715..5383
                     /gene="sdaAB"
                     /locus_tag="MBZ71_000626"
     CDS             4715..5383
                     /gene="sdaAB"
                     /locus_tag="MBZ71_000626"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBT4428602.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5396..6268
                     /gene="sdaAA"
                     /locus_tag="MBZ71_000627"
     CDS             5396..6268
                     /gene="sdaAA"
                     /locus_tag="MBZ71_000627"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBT4428603.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6359..7378)
                     /locus_tag="MBZ71_000628"
     CDS             complement(6359..7378)
                     /locus_tag="MBZ71_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBT4428604.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7551..8456)
                     /locus_tag="MBZ71_000629"
     CDS             complement(7551..8456)
                     /locus_tag="MBZ71_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBT4428605.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8515..9060)
                     /gene="nusG"
                     /locus_tag="MBZ71_000630"
     CDS             complement(8515..9060)
                     /gene="nusG"
                     /locus_tag="MBZ71_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBT4428606.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9161..9331)
                     /gene="secE"
                     /locus_tag="MBZ71_000631"
     CDS             complement(9161..9331)
                     /gene="secE"
                     /locus_tag="MBZ71_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBT4428607.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9355..9507)
                     /gene="rpmG"
                     /locus_tag="MBZ71_000632"
     CDS             complement(9355..9507)
                     /gene="rpmG"
                     /locus_tag="MBZ71_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBT4428608.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9669..10313
                     /locus_tag="MBZ71_000633"
     CDS             9669..10313
                     /locus_tag="MBZ71_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4428609.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10369..10872)
                     /locus_tag="MBZ71_000634"
     CDS             complement(10369..10872)
                     /locus_tag="MBZ71_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBT4428610.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10885..10931)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11469..12434
                     /locus_tag="MBZ71_000635"
     CDS             11469..12434
                     /locus_tag="MBZ71_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBT4428611.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12533..13705)
                     /locus_tag="MBZ71_000636"
     CDS             complement(12533..13705)
                     /locus_tag="MBZ71_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428612.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13692..14837)
                     /locus_tag="MBZ71_000637"
     CDS             complement(13692..14837)
                     /locus_tag="MBZ71_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428613.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14851..15783)
                     /locus_tag="MBZ71_000638"
     CDS             complement(14851..15783)
                     /locus_tag="MBZ71_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428614.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15900..16979
                     /locus_tag="MBZ71_000639"
     CDS             15900..16979
                     /locus_tag="MBZ71_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBT4428615.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            16976..17605
                     /locus_tag="MBZ71_000640"
     CDS             16976..17605
                     /locus_tag="MBZ71_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4428616.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17715..17942)
                     /locus_tag="MBZ71_000641"
     CDS             complement(17715..17942)
                     /locus_tag="MBZ71_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4428617.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18045..20633)
                     /locus_tag="MBZ71_000642"
     CDS             complement(18045..20633)
                     /locus_tag="MBZ71_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBT4428618.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20653..21312)
                     /locus_tag="MBZ71_000643"
     CDS             complement(20653..21312)
                     /locus_tag="MBZ71_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4428619.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21361..21864)
                     /gene="trmL"
                     /locus_tag="MBZ71_000644"
     CDS             complement(21361..21864)
                     /gene="trmL"
                     /locus_tag="MBZ71_000644"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBT4428620.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22085..22951)
                     /locus_tag="MBZ71_000645"
     CDS             complement(22085..22951)
                     /locus_tag="MBZ71_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4428621.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23060..23701
                     /locus_tag="MBZ71_000646"
     CDS             23060..23701
                     /locus_tag="MBZ71_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBT4428622.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23763..25124)
                     /gene="mgtE"
                     /locus_tag="MBZ71_000647"
     CDS             complement(23763..25124)
                     /gene="mgtE"
                     /locus_tag="MBZ71_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBT4428623.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25142..26041)
                     /locus_tag="MBZ71_000648"
     CDS             complement(25142..26041)
                     /locus_tag="MBZ71_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBT4428624.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26043..26840)
                     /locus_tag="MBZ71_000649"
     CDS             complement(26043..26840)
                     /locus_tag="MBZ71_000649"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBT4428625.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26818..27522)
                     /locus_tag="MBZ71_000650"
     CDS             complement(26818..27522)
                     /locus_tag="MBZ71_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBT4428626.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27653..28228
                     /locus_tag="MBZ71_000651"
     CDS             27653..28228
                     /locus_tag="MBZ71_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBT4428627.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28346..29023
                     /locus_tag="MBZ71_000652"
     CDS             28346..29023
                     /locus_tag="MBZ71_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBT4428628.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8547 a         6146 c         5221 g         9112 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tgttcatcat ctcttcactt
     2941 cgttcatagt gattcacaat aaaaaactct atgccaccgc agacatagag ggactattga
     3001 atatattcaa taaacgtcct cggtaggaaa ttaaggcttg atgccaccta ctgtcttcgg
     3061 tacaagttaa ttattaacct caactagctt agcatgctaa actagttgag tcaagaatta
     3121 tttttcagtt ggatcaatca acattctaat atttaattag aaagaagctt ggtcaacatg
     3181 gataccaggt ccaaatgttg ttgtgatcgt tacatttttc atgtattgac ctttagctgc
     3241 tgatggttta gcttttagca atacatcatg gattgctgca aagttttcaa ctaatttttc
     3301 atcgtcaaat gaaacttttc cgattggtac atggatattt ccagctttgt ccacacggta
     3361 agttacttta ccagctttca cttcgtttac tgcttttgtt acgtccattg taactgtacc
     3421 agttttaggg tttggcatta agccttttgg tcctaatact cgtcctagac gacctacagt
     3481 agccatcata tcaggtgttg caacaactac atcaaagtca aaccatccgt tttggatttt
     3541 ttgaaccatg tcgtcgtcac ctacgaaatc tgcaccagct gcttcagctt cttttgcttt
     3601 gtctccttta gcaaaaacta aaacagtttg tgttttacca gttccgtttg gtaatacaac
     3661 agcaccacgg atttgttggt ctgctttttt agggtctacg ttcaaacggt atgcaacttc
     3721 aactgttgca tcaaattttg caatgtttgt ttcttttgct aaagctactg cttcggcaac
     3781 aggataaact ttacttgaat caactttttt taatgcatct tgcatttttt tgctcttttt
     3841 agccattttg gttcctcctt gattgtggtt ataacggttg tacctcccac gtgttctata
     3901 tctttcaata tagagccaac tgagaagctg ctgtcttagg gtgtatagat tattctacag
     3961 tgatccccat gcttcgtgca gttccttcaa ccatgcgcat tgctgcttca acgttagctg
     4021 cgtttaggtc ttccattttt aattcagcga tttctcttac ttgatcgcta gaaactttcg
     4081 caactttatt tttgtttggt tcgcctgaac ctttttcgat tttagctgct tttttcaata
     4141 gaacagcagc tggcggtgtt tttgtaacga atgtgaatga acggtcttcg tatacagaaa
     4201 taacaaccgg aatgatcaaa cctgcttgat cagctgtacg agcgttgaat tcttttgtga
     4261 atcccatgat attgataccc gcttgaccta gtgcaggacc tactggtgga gctggagttg
     4321 ctttacctgc aggaatttgc aatttaacga ttttttctac tttctttgcc acgagacata
     4381 cctccttaag tccgtgatgt ggttaattgg agatgcatat ttctcctccc acgttttatc
     4441 tgatctgaaa aaatccagac aaataatgtg caaaacaatg cacactggta cagtatagca
     4501 aatttatttg ttttttcaag atttttttct gctattttta cattttgctt gaataaacgc
     4561 taaatttatt ctaaaaaaac aacaattttt cgttctcatt tcttctttta tgttacactt
     4621 gcgctagcat ggttcaacag atgaaagcaa acaataacac ggttttttag acccttgtac
     4681 aaagattcct acataacaaa atgaggtgga gaaaatgaaa gatttaaggt atagaagtgt
     4741 ctttgatatt attggacctg tgatgattgg tccaagtagc tctcacactg ccggagctgc
     4801 tagaatcggg aaaatcatac gtagtatatt tggcgaacag cctgattctg tagatattta
     4861 tctatatgaa tcatttgcaa aaacctatcg ggggcacgga acagatattg cattagttgg
     4921 cggcctgtta gggatggaac ccgatgatga acgtttagct gattctttga aattggcata
     4981 tgaagccggt atggaagtct tgtttgttcc taagagtgaa aaagccgaac atccaaactc
     5041 tgtaaagatg cttctaaaaa aaggaacacg aaagctttca gtcactggca tttcgatcgg
     5101 cggtggcaat atccagattt ctgaattgaa tggattcaaa atttctttaa gcatgggtac
     5161 tcctaccttg atcgttgtcc accaagatgt tccaggtatg attgctaaag tgactagtat
     5221 actttctgag accaatacga atatcggcac tatgacagtg acacgggaat caaaaggtga
     5281 aaatgccatt atgataattg aagtagatga tcctcaagta gaagaaacgg taaaaagact
     5341 caaacagctg ccaaatatcg acagcgtaaa ttatttcgat taaggagcta aaaaaatgtt
     5401 tttatcgatt gaagaattag tagatcaagc aaaagcttat ccaagtgtag cagaattgat
     5461 gattgcagtt gaaatggaaa tgagtggccg ttcccgtagt cacatcatcg agatcatgga
     5521 aaaaaacctc tcagtaatgg aacaatcaat caacgaggga aaagaaggtg ttacctccgt
     5581 cacaggaatt accggtggtg atgcaaaaaa attagatgat tatttaaatg aggcagactt
     5641 tttaagtgga gaaacaatct taaaagctgt acgcaatgct gtagctgtta atgaagtcaa
     5701 tgcaaaaatg gggttgattt gtgctacacc gactgccgga agcgcgggtg ttgttcctgg
     5761 tgtgctgatg gctgtccgag atcggcttga tttgacacat gaacagcaat tggaattctt
     5821 atttaccgct ggcgctttcg ggttagtcat cgcaaacaac gcttctatca gtggtgcaga
     5881 agggggatgt caagcagaag ttggttctgc aagtgcaatg gcagcagcag ctttggtttg
     5941 tgcaaaaaat gggactcctc atcaagcagc acaagcagta gccattacat tgaaaaatat
     6001 gatgggactg atttgcgatc ccgttgcagg cttagttgaa gtcccgtgtg tcaaacgtaa
     6061 tgctttaggc tcttctcaag cttttatctc tgctgatatg gcattagcgg gtatcgaaag
     6121 tgtcattccg ccggatgaag tcgttgctgc aatgtaccaa gtcggacgtc aaatgccgtc
     6181 gattttcaaa gaaacagcag aaggtggctt agctgttacg ccaactgcta aacgacttgc
     6241 ggaagagatt ttaaataaga gggcgtgata aagctcctta atttcgagta ttaagagatt
     6301 agttatcttc ataacaaaaa acgaagagag gactaattgc ccttctcttc gttttttatt
     6361 aatccatcaa tttcgtattt tttacttcat cgatcgtttt tgttcctgca agttgcatcg
     6421 tgatcgacaa ttctttgttc aggtgttcaa aaacagatgt tactccttct gccccaccta
     6481 gattcaaccc ataaatcact ggtcggccaa tcgcaactaa gtcagcacca cttgccagag
     6541 ctttgaagac atgctctcct cgacggacac cagagtcgaa aatcactggt acacgtttgt
     6601 tgactacttc ggcaattttt ggcaatactt caaaggacgc aggacctcca tctaattgac
     6661 gtccaccatg attggatacc cagataccat ctgcccctgc agagatagcg attactgcat
     6721 cttctggcga ttgtattcct tttacgataa ctggaagatg ggtgatttct ttgattgttt
     6781 tgatgtcatc tggtgtcaag ccttgttttg cagctgcata aatttctgcg attccttttc
     6841 cttcgccatt accacttgca ctttgttcgc tatacgcagc taagttaggc attggtaatg
     6901 gaaattggaa ttgattgatg acgtcttctt cacggtaacc accgagcgtt gaatctgccg
     6961 ttaaaatgat agctttagcc cctgcttcta ctgctttgtt caaaatgaac tcattgaatc
     7021 tgtcgtcttt actcatatat aattggaaaa actgcggtgc gcctggcgct gcatcggctg
     7081 cgtctttgat tgtcgtattt gcataagtgc tgatggaaaa gattgatcca gcagcagcta
     7141 ctcctttagc tgtatcagct tccccttttt catgtgccaa tccttgggcc gctgatggcg
     7201 cttggataat cggtgtatct aattcaattc cgaaaacaga tgtatgcaaa tcggctgagt
     7261 cgattccgcg taatatccgt ggcatgattt tctttgtatt aaaagaagtc gtattttctt
     7321 tcatcgtcca ctcatcttca gaaccaccgc gaatatagcc aaaagctcct gcttccatgc
     7381 gctcttttac acgaccttct agttaggcaa tgtttacaat atcgattgga tgttcttcat
     7441 tacttgtatg ataagtcata gtagatcctt ctttcatctt cttgattaca tataaatcat
     7501 actaaactta caaaaagtaa gcaaacattt actctcagat acttttatcg ctattctttc
     7561 caaataaatc gacttacaag ctgatctact tcttggtttt catgaagcat actatgagag
     7621 gcattttcac ctgtcactac tttttcctct atttcatttc cgcgttgctt caacaacgca
     7681 tagaccgcta atgcactgtt taacggtact gttccatcac tcaagtctgt ttcatctagt
     7741 tgaccagcaa tcaagaaaaa tcgagtggtt ataggaatat taccgatcat ttgctggtaa
     7801 tcttgatagc ggttagaaac gacagcaggc ccctttgcta actcatctgt cagagactgc
     7861 gcactatcat cgacaaaatc attaaaaggc gcgccaattg ctgttaatgt ctgaacttgt
     7921 ggcaatgacg tatcctgtcc atacgttcct aaatagcgta gtgcactaac tcctcccatg
     7981 gaatgcccca ctatgttgac tttatctatg ccgtagtttt ctttcagata caaaagacaa
     8041 gttttgatcc attctgcttg attccactcg ttatttttat tatcttcaaa caatacttgg
     8101 accatcggat tgtctgcttt tccagacaaa tctcctgttg cttgtatttg tccatctgtt
     8161 cctactgtta aaaccaattc ttttactgct tgctgatttt tttccaaacg ccgcaacatc
     8221 cctttaaagg aattttctgt cccgctgtag ccgtggaaaa aaagtgttgg aatagtggtt
     8281 ttttctatca ctttactgga ttctgccgtt ttttctttat cagaaacttc agtagtattt
     8341 atcttcgtcg tctggcaacc acttaacgca aacaaactga taaggactaa agctgctgca
     8401 gttgcacgtt tgcataaatt tttgattggt tctgtcatac acctcttcct ttccatatag
     8461 aaaaaagaaa gacttgaaag gccaatcata tgatcgacat ttcaagtctc tttattaatc
     8521 gattttatct acttgatcga aatcaagttc tgtactggtt tcacgtccaa acatatcgat
     8581 gttgactttc aatttttctt tttctccgtc aacttctgtt acttgtcctt ctaatccagc
     8641 aaatgcgcct tcgatgattt tcacgacttc gccaacttct acttctagat cattttgtcg
     8701 caggctcata ccgatagaac gcaagatatg attgacttct tctggaagta atggtgctgg
     8761 tttgcttccg gctccgtgtg aaccaacgaa tcctgtcaca ccaggtgtgt tgcggacaac
     8821 ataccatgaa gcgtctgtca tgatcatttc aacaagtacg tatcctggga atgttttatg
     8881 aacgatttct ttttctttcc catttttgac ttcagtttct ttttcttcag gaacaacgac
     8941 acggaaaata aagtcggcca ttttcatact ttgcgcacga gattcgatat ttgcttttac
     9001 tttgttttca tatcctgaat atgtatgcaa gacataccag tttttttcaa aagattccat
     9061 tgaaggctcc tcatttcatt gtaaaataaa aaaacctcag tacacccgaa gtttttcttt
     9121 cgcttcatta tagcactaaa agtacgttgt ggctagcgag ttattttaaa atccagccaa
     9181 ataagctttg gatggctgta tccattacgt aaaacatcaa tccgaaaata atagaagttt
     9241 cgatgactac taacgtatct ttacggagtt gttttctgct tggccaagtt acttgtttga
     9301 tttcatcttt tacgcttttt agaaatttca tgagacaacc tcccttgcta ctgtttattt
     9361 cgtttcttta tgtaatgtat attgattaca atatttacaa aacttattga tttccaaacg
     9421 ttcgccgctt tttccttcac tgactgattt tgtgtagttg cgagacccac aaacggaaca
     9481 agcgagtgct gcttttttct ttgccattta cttctgcccc cttgctgaga atcagactta
     9541 tacttcataa cagtatcacg taacttttta cttgtcaatt tgtgaaataa actatctatt
     9601 gttttggata aaattatgat atgattaatt tgaaattaag ctaaaatgag acaattggag
     9661 gtatagcgat gctattaaaa acacttgtat ataagaaaaa agatctgaca accgttacag
     9721 agactagtac attagaagaa gcattaaaaa ttttagaaac ttccggatac cgttgtgtgc
     9781 cgatcttaga ttcttcaggt aatattttcc gtggaaacat ttacaaaatg catatctatc
     9841 gtcataaagc aaatggcggt gatatgacgc ttcctgtcac tcatcttttg aaaaacgcta
     9901 caaagttcat ttacctggat acttctttct tcaaagtatt ctttacgatc aaagagcttc
     9961 cttatattgc tgtccttgac agtgaaaatc atttttatgg tattttgaca catagtacgt
    10021 tgcttaatat gctctctcaa tcttggagcg tagaacaagg aagctatgta ctgacaattg
    10081 cttccacagg aaagcaagga gatttggctg ctattgcaaa aatcatttca aaacacagca
    10141 gcattgccag ctgtattact cttgatatcg aaaaagacga atacattcga cgtacattga
    10201 tcacgctacc ggctaacact gaagcgaaag tatgtgaaac gatcgtcaac cacttagaga
    10261 aaaagaatta taaagtggta gaaattgaaa atttgcaaaa ccaagaagca taatcaaaaa
    10321 gcgaaaaaag aaaacagcta atctgctttc ttttttcgct ttttataatt agacacgctt
    10381 ttcaaaatca agcgcgcgat ctaaataata catcaatggt gctgaaacag ccatgacgat
    10441 aaactcactt aatgcaagcg tgccataggt tgtccaaaat gcttggccac ctgttggaac
    10501 taacatataa gcaatcagtc ccatgctgat ggtaaagaag atcgtgttca atactaaacg
    10561 catctttaca tttttcactt tgttttgcaa taaagctgtc aggcctaaag aaagcaatgt
    10621 ttggccgcca ccaaaaacca catccatgat accaaaacca aagaaggcat tatagacgat
    10681 gaccccgcct aaaactcccc aaagcaattt acggttaaag acaactaaat gattcaaact
    10741 ttctgaaata cgaaactgga tcggccctga ggatacaggc gcaactaaaa tagttaacgc
    10801 tagataaaga gccatgatga tgccatttac aacgattacg gataaccgtt gtctgctttt
    10861 cgttttttgc attttctctc ctcttttctc ctagtttttt taacgcggga tggttgatga
    10921 accgcggtta tgtgaaacaa acaataatta taacagactt ttcatttatt tcaatccgtc
    10981 ttatctgctt ttaaaaaagc aaaagcttaa acaatgtaaa cgtttgctaa aaatcgtggg
    11041 atatggtata aagtaagtgt aaggggtcat aagaattccc agtaaagcag acgaagtagg
    11101 atctgcaaga acagaattgt tgtgaaactt aaataaataa aaaaggatgt gggaatttga
    11161 gcaacctctt actagagaac ggtctagcaa gcagctgaag atctaagtgg acatgccgaa
    11221 ccttggaact tctccttacg taagtaatgg atcatgcgct tgctaggctt ttttgtgttt
    11281 ttcggtcttc ggaccttttt cattttccta gacttctgct ataataacca agaattaatt
    11341 ttgtaacaga tttttttgaa aaaacaagtt tctttcattc aaaaaataga aaaagcttgc
    11401 taattattgg ttaaccagca aacaggagta ttaaaatatc ttgttttatt gaatagcagg
    11461 aggaaatcat gaaattccat tatcatatat tgatcaatcc atcggcaggc agcggaaatg
    11521 gctataaagt agcagaaaga attttaccgg tattaaaaaa taagcatatt gactatacac
    11581 ttcattatag tgaatacaaa ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa
    11641 cattgattcc ttggatagaa gaagaacaga aaacaatcga tactttcccc cttttgatca
    11701 tcgtaggagg tgacggcacc ttacatcagg tacttgatac tttctatcag atggaagtag
    11761 aattcccagt tgcttatatt ccagcaggtt ctggaaatga ttttgctcga ggggcaggcc
    11821 ttccaaaaaa tcccaaaaaa ggacttcagc tgattttagc agctcaatca ccagaaaaag
    11881 tccacataat ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt
    11941 tcggtatagg attagatgca gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc
    12001 gattgaacaa atacaactta ggatcatttt cgtatttatt ctctattctc cgtgctttat
    12061 tcactcaaaa aggatttcca attctggtag atgcaggagg aaaacaatta agtttttcaa
    12121 acgcattttt atgtacagca acgaagcatc ctttttttgg cggaggaatc gcgattgttc
    12181 caactgctga tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga atcaatttct
    12241 ttaaaatctt gtggctcatt cttttactta tacagaaaaa acaattgaaa tcgaaatatt
    12301 tccatcacta cacaacaaac agattacgta tcgtttcaac aacaccgcaa catggtcaag
    12361 aagatggaga agaaatggaa aaacgacctt ttgatatcac aataaccaca actaaacagt
    12421 tgttttggtg ttaataaaag aggttttgtc agaagctaca tctggaacac cgtttattcg
    12481 gaaaaaagga ctgtgacacg acttttgtca cagtcctttt tttactatgc agttaattct
    12541 ttgcccaatt gggttgccat cgatagtttc tttctttacc caaaataaat tggatcattg
    12601 aaaacaccgc agcaaaagca actaagacac aaccattgaa ccaaaaagat tctgcaaatg
    12661 tttgattgta actgagtgtt tcactgatca agttattatt ttgaacaaac caatatgttg
    12721 gtgtgaatgc cgctattttg ctaaccattt ctggcaagta ttctgctggt acaaacaccc
    12781 caccgataaa gcaactgcct gtgataaaaa tatctttgat cccaccgctg gtttctgagt
    12841 tttttaccaa tgaagtgata caagccgaaa agctaatgat cggcaataaa aacaagaaag
    12901 tatttaaaat atgataccat accgttatat cccacgaggt gtggatcact aacagaacca
    12961 ttagaagtaa gaagccactg aacaacagca gcgagtaact aatcaaacta aaggttactt
    13021 tgcgattcaa gtttcgtttg gtaatgggcg cactctggtt tcgtttactg atctctttgc
    13081 gattaaacgc tagattgatg acgctgaaac cgctaaaaat ggatagaaac aacccataag
    13141 acaagaaatt aaaggttgtg cccgtcaaca gctttttcat ccgctgacta tatcccgcac
    13201 ttagctcaac ttctccagta accgagagtg ccatttgtgt ctgttccata gctgcatcga
    13261 ttgttttatc ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg
    13321 taacaactgt tttagaaaag gcatctgggc gactctgtat cttgatcgtc gccatctttc
    13381 ctacttcgac ttgttcggta aagtctgcag gcagatgtaa gataatgttt acacgattaa
    13441 aataaagagc atcatcgatt tctttttgtg aggttccatc caatgtaacg actgtttcct
    13501 tgtcattcaa atatttacga aggccatcga ctacaggcga ctcttcttca ctaatcaagg
    13561 cgattttcgc atcttgcaga gaggtatttt caacatcaat attttgcgca aaaaagaatg
    13621 tggcaacaac agtgataatt acacctatca gtaaattcga tttatttgct ttaagcaatg
    13681 tccaaatcgt tttataaagc gtcataacgc acccctcttt ccatccaagc atttcctaac
    13741 gcaaaaagga aaataaatcc gatcaaccat agaagattgg tataaaaact agccaaatca
    13801 gtgtagaaat aaagtttata caaactttcg ctgatcaagt tgacgagatt taattgacca
    13861 acaatcggaa tatgaagatc aatccaatac ttcacttgtt ccgaccccat cataccagca
    13921 aaaaagctca tcgcaatcgt cactgaaatc aagatgctat ccttttgcac cagattcatt
    13981 tttggtatca aattaccaac caatgtacct aacaaaatcg cacacaacga ccccagagcg
    14041 gtaacaatca tcaaccattg ccaacgttca ccaaagtcta cttgatagat aaaacgaaag
    14101 acagctaaca taatataaat ctctgtcaaa acaatcgtaa agcttgctgc caaattagcg
    14161 attgaaacta acgattgctt tctaggaatc aaggacaagc gaataccatt tgacgattga
    14221 tttgcttgct gatcctgtac attgcggacc ccccacatcg tcccgtagag aatcgccatt
    14281 ccaatcagag tgaagaaata aaaacttttg aaactcatat tacgacctgc atgaacttct
    14341 tgaaaagata cttcttgttc aaaaacagtc atgatgggcg cgagatcaac gttctccact
    14401 tctgccactt tactcgaaat tataccaata cgctgtaaaa attgatccat gacgtttttc
    14461 atcaacgtct gactcattcc tgcttgaccc acatggagcg aaacagatga accatcaatg
    14521 agataaaaac cagccaaatc atctgcattt agcttttcat tcgcttctac ttccgatagt
    14581 tcctgtactt caaataatgg tttatcattg acttcgactt gttctaagac tgtggtaaaa
    14641 ttttccgaaa gcgctgtttg atccgctttg attacaattc cagtattaat cgtttcaaga
    14701 ttgccacctt gatcaatctc gccaaaggcc gccataaaca tcaatcctaa aagaattgga
    14761 aaggccaatg tccaaaacaa catggattta tttcgcagca gtatttttgt gcgatattta
    14821 taaaaatgcc aaaacattat ttcccctcct ctagtctctc aaatccttgc cagtaatctc
    14881 taaaaagaca tcattcaagg acgcttcttt ggttgctatt tttccgtaac tgatcttttc
    14941 ttcttgtaag attgtaagaa tcggcaacaa tactgtattt agttcagtag ttgttacttg
    15001 gagctgacct tttttataag cgattccatc agaagctatt gttcgctgaa tcgctgacaa
    15061 cacattttct gggagtaatg ggatgtcgat cgtcaaacga tccgccgtcc gtaccatatc
    15121 ttttagagat tctttggttc ctttagcaat cacctgtcct tgatccatga tcacgatctg
    15181 gtcgcaaaga atttctactt cttccatgta atgtgttgta tagacaatgg ttgctccttg
    15241 tcgattcagc tccttgatac tttctaaaat tttattccga ctttgggggt ctacagctac
    15301 tgtcggctca tctaaaaaaa ttaaccgcgg cttgtgggca atcccacatg cgatgttcaa
    15361 ccgacgtttc aagccaccac taagttctcc tggacgaaac gaaacatatt tttctaatcc
    15421 cactaattga atcacctctt caatcaaggc tttccttttc tctttttcgg aaacatataa
    15481 accgcaaaaa taatcgatat tttcttgcac acttagttcg tcaaagaccg caacgtcttg
    15541 aggaacgacc ccaatcattg ccttgacagc ataattttca ggagtcattg ttgtcccgaa
    15601 caatttaatg gttcctttgt cataatttaa caaagaaagc atacagttga tggcagtcga
    15661 tttcccactg ccgtttggac ctaacagacc taagacttct ccttcttgta cttgcagatc
    15721 aaaatgattc aatgctacaa aatctccata gcgtttgact aatttatcaa tttcgataat
    15781 catcatgaaa aactccttcc aaatttcttg ttgatctgac tataataaag tgcaagaaaa
    15841 gaaactagtg acgattggct aaaccacaca tgacatttgt cacaatgaag gagtctgcta
    15901 tgcataaatc tcggctactc aacgaaacta gctttctact ggttatctca ttgcttttgc
    15961 tggtaagtca aggattcgtt gctatgcaag ttatctattt attgatcgcc gttttattca
    16021 ctggtgtcag ttatttactg cctacaaagc caaattggat tttccaagtt attctatttc
    16081 ttttatgtgc ctttttacct gatcttgtcc tgtttctgcc agcgacgatg cgagtgatcg
    16141 cccctgaaga aagccacctt gattacttgt ttttgttagg ttttttgttg atttcgacgg
    16201 ttggtaatca tccccccatc aaggtcgcct ttgtcgtaat tttgctgttt ttttcctttt
    16261 acttacgaaa aaaagatcaa cgaacgaccg atcttgagaa aaagtacatt gtctccaaag
    16321 atactcattg ggaaaaacaa caagagttat cggaacaaaa taaagcattg gccctgtcac
    16381 aagaaacaca attagccttg caaattgtgg aagaacggaa ccgaattgcc cgagacatcc
    16441 atgacaatgt cggtcatctg ctgtcaagtg cgattttaca aattggtgcg cttgaaatga
    16501 tcaatcaaga accaaaacta aaagaaccat tagaaaattt aagtgccacc gttcacactg
    16561 ggatggatcg gattcgtcaa agtgtccatg acctccatga gacatcgatc tcttttcaac
    16621 actccttaga gtttctgatc gctgattttc cttcttctgt tacgattgaa ggaaacttgt
    16681 ttgaaggatt gaatgaaacg cagcaaaact gcttcttaat ggtcatcaaa gaagccttaa
    16741 ccaacagtat caaacatagt catgccaaaa ccgttgtttt acattttcga acgttgccgg
    16801 ccttttatcg cttgcaaatc acgaatgatg gcaccagccc acaaaaaaat tctgctcccg
    16861 gtattggttt gaaaacgatg cgtcaacgtg ttcaagaaca aaagggacaa gtccatattt
    16921 tccaagaaaa ccagcaattt cagttaacga tcattttacc aagggaggga caccaatgat
    16981 aacagtagtc attattgatg atgatccatt tgtgacgaaa tcgttacaaa ccattttaga
    17041 aagcacgaaa gagatccgtg tgttaggcat tggtcattgt gccaaggatg cgctagtctt
    17101 atacgaaacc catcgtcccg atgttttgct gacggacatc cgcatgccta aacaaacggg
    17161 catcgatgct gccaaagaaa ttttggctaa atttcccaaa gccatccttt tgcttttgac
    17221 gacattcaaa gatgaagaat acatccgcga agcattttcc atcggtgtca aagggtattt
    17281 aatcaaacag aacttgcaag cgattattcc ttctgtcaaa tccgcttata atggacaggc
    17341 cgtatttggt aatgaaatca tcgagacttt cactcaaatg atgaagaata aaccgacgat
    17401 tgatagtcat gcttatgctt cttttagtga acgtgagctt gcaatcatca aagaagtagc
    17461 tgccgggaaa aacaataaag aaattgcaga tgccttgtac ttgagtgacg gcactgttcg
    17521 caattacatc agccaattac tagaaaagct agatttacgg gatcgcacgc aattggctat
    17581 ctatttttat cagcatttgc aataagtctt atctaacaca aaaaaacctg aatgatgctc
    17641 cttcgcatca ttcaggtttt tttcacatct tctattcaat tgtcccgcaa accatttcct
    17701 cacgattgaa agaatcaagg attcgcaggg ctgccaggat tcgttggatt gttcggcgtg
    17761 ttaggcggaa aaataggcgc cttgtctaac gttttttcta gttttgattc ttcacgatcg
    17821 tctcgttcta catcaggact ttcattcaat ttgaattctt tttccatctc atctaacttt
    17881 ttaaagtcat tgccagattc ttttgctaat ttcgctaatt tatcttcggt ttctttagcc
    17941 ataaagtaac gccccccttt tgatttcagc ttagcatatc acactgaaaa aacaaaagaa
    18001 aacgctaacc aacgatcatt tttagctatt tctcatcttt aaaatcaagc attttttgcc
    18061 ggtataaaat cgtagggaga actgttttcc ataccaatca tcggatctac taactggtta
    18121 tcgatcactt ctgctgtaag aacgaaatct ttagaaacag caggttctac ctcttctttc
    18181 ttttcttctc caacaaatga agaaaacaga tctggttcca ctgtttccat tacttggttt
    18241 ttcgtttcat tcgtcccact gtcatcttct acaggtagct tttcttttac aacccgtggc
    18301 tcttttgtat agttcccatc ttcaaatgga tcatttccga ccaaagaatc ttcataagct
    18361 gctagtttgg tccggagagt gatattcatt ttatcattat ccatgatcct tgatttcaac
    18421 gtagtcatcc ggcttgctga ttcattcgct acacaagtga cataggcttg ctcttcccct
    18481 aacaaaatca acagttcctt actgcgagtc actgccgtgt agagcaaatt acgctgaagc
    18541 atccgctgat attgatggac catgggcaaa ataaccatct tgaattcact gccttgtgat
    18601 ttatggattg agcaacaata agaaagagtg attttattcc attcgtttct tttataagtg
    18661 acctcatttg catcaaactg gataactaat tcatccactt tatcttcaga atcttttgcc
    18721 aagatgatac ccgtgatttc tcccatatct ccattgaata cattcaattc gggagtattg
    18781 actaattgga ggactttgtc tcctatccga taaacggtgt cgttccaatg tacttccttt
    18841 tttccaggtt tccctggatt gaatatctcc tgcatcattt tatttaacgc atcgatccct
    18901 gcaggtcccc gatacattgg tgctaatact tggatatctt gtgcacggaa tcccttattt
    18961 ttcgcttttt ccacaatttt agagatcaag ggttcaattt gataagcatc acttcggaaa
    19021 taggaacgat ctttttgatt ctttgtgaaa tcttttggca gacgtccttc tttgatctca
    19081 tgggcaagag gaataatact agaaccatca ccttgccgat aaatctgatt gagttccatt
    19141 ttgggaattt gttcgatttc caaaagatca tggagtactt gaccaggacc gacagaggga
    19201 agctgatctt tgtctcctac taagataact tgcatattcg tcggaatcgc tttgaataat
    19261 gtgttagcca gccatgtatc gaccatagac atctcatcaa caatcaaaag tccgccttct
    19321 aattctttcg tactgactga aggtgttttt tcccgtccat tcagccccag aagacgatgg
    19381 atcgtactgg caggcagacc tgtcgtttcg ttcattcgtt tagctgctcg gccagttggt
    19441 gctgcaagta gaataggaaa aatttcttgg gtatattttg aaggatccaa ttccagtccg
    19501 ttcaactctg caaaaagatt gacgattccg ttgatcacag tagttttccc cgtacctggt
    19561 cctcctgtta aaataaatag aggggaacgg attgcttcag cgatagcttg ttcttgcgaa
    19621 ggtccatact gtatgccgaa tattttttcg atacgccgaa tattcttctg cacttctttt
    19681 tcaggataat ggatttcttt tttacgactg agaaggcgct ggatcgaatt accgatcccc
    19741 cattcagaaa agtacaaact gttttcgtaa agattagtct cttcttgttg tatctttcct
    19801 tcttcaacaa gttggataat tacagtagca acctgatcag gctggatttc aattgggcga
    19861 cttgtttcta aaatacggat cgtttgctga agcaatgtct ccgccggtac gtatgtatct
    19921 cctgtttgaa ctgcatgctg aaaaatctga tgcaagatag ctgctcgaat tcttctcggt
    19981 gaatctgctg caataccgat ttgttcggct agattatctg cacgtttgaa cccgattcct
    20041 tctatgtctt cgactagctg gtacggattt tcttcgatga tatccaaaga ctcgttctta
    20101 taggcctggt agatactgaa agctaactga ctaccgaacc cgtaacgatt cagtcccatg
    20161 atcacttgat ccatcccgtg gtttagccga atcgtctcag caagcatttc tctttttggc
    20221 ttcgtcaaac cggagatttg ttttaatacc gaaggatcag caatgatttt ttcgatagca
    20281 tcttctccca gcaattctac aattttttct gctgttttct tcccgattcc tgggaatttt
    20341 tcgctggaaa gatacgctac gatcccattt gcagttgttg gcttttcttg ctcatatctt
    20401 tcgagtttaa gctgccttcc gtatttagga tgatccacta attctccata aaaacgataa
    20461 gtctcatttt cttgaatatc gccgaaactt ccggtgacta caatctcttt ttcacggtaa
    20521 tcagctgtgg tttcactgat gtgtaccaaa agcactttgt aaaaattact aggattttga
    20581 aaaaaaatcg cggcaactgt accaacgaaa taaggcattt cttctggctg catcggaagc
    20641 cgcctccttc ttctatagta aaggttctgg tgctgtttca gttcctaaaa aatctgtttg
    20701 gttccatact ttcagttcat atggcaataa tctgcaaggc tcttcagtct ctaaaatcgt
    20761 caaactacta ttgaacaatc cacccatttc acgcaactca cttagctctt ttcctgtcat
    20821 ccattgaatg gctgcagtta aagaagctcc atgtcctacg aataaaagtg ggccatcttt
    20881 atgtcgggca accgcatctg ctacaacagt ttcgatacgt gtgattgccg attcaatcgt
    20941 ttctccgtga aaaggagaag gatcatacag atctaaatga ttacgcaaat tcggcagatt
    21001 atccggatat ttctggtaca tttcatcgat cgactgacct tctaaagtac ccagccctag
    21061 ttctcttagc ttatctgtat aaaaaatctc taccggacga gcaagctctt tgttgatacc
    21121 ttctgctgta tcacgtgctc gctttgctgt actggaataa attgctttaa aaggaacata
    21181 ttttaccttt ttcccaaatg ctttgatttc ttcgtaactt tgtggcaaaa gcggagaatc
    21241 gccattgctc ccttgaaaac gtctttcctg attccattgt gtcttcccat gtcgggtaaa
    21301 atatagttcc atacgcagca aactcctgtc tttcttttat tttactctat aataaactgt
    21361 ctaatccagc ttgtcatttt catagtgatg cgtcaattct aaattaggga atccttgctg
    21421 gcgaagtgct tcatacacta tcattgcagc tgtattcgat aaattcaatg agcggacatg
    21481 ctcatcattc atagggatgc gtaaacactt ttcttcgttt tcacgcataa atgtttctgg
    21541 caaaccggtt gtttcttttc caaataaaaa gaagtgctct tctccatcag caaaattttc
    21601 gtcactatat actttatggg caaattttga gatcaaatgt agtttacgat tacctaaaaa
    21661 tgttaaaaaa gcttctaaat tactgtggta agtaatattt acatcattcc aataatccaa
    21721 ccctgcccgt tttagatgtt tgtcatcagt tgaaaaacct agtggctcga ttaaatgaag
    21781 cggagtattc gtggctgcac acgtacgggc aatatttcca gtgtttgcag ggattaatgg
    21841 ttcaaataaa acgatatgat tcatcaggat cattcctctc atatttatgt tcgtttctat
    21901 tttctgacaa tttgattttt gcataaaaaa aaacgtatca actcggtgtc agtcactgcg
    21961 agtcgttacg ttagtgaagc tcttctcact aacttttatc agaacaggaa cctgataaaa
    22021 accaatgaaa aaacaacttg ttgctaatat aacaccctca actttttttt gcaacctcta
    22081 ctcattaaaa actgctagaa aaacgttttc tcttagttcc ttttaataat tctatgtcaa
    22141 ttgtaatctt cttcaaagga ttcttatata agtctgcttt gattcgctct tctacctgcc
    22201 attctaaagg tgacatggcc aaaagatctt tttgattttc ctctggtatc tcaaattcat
    22261 aggttacacg ctgacgttca gttaatttca ttgcttcagc aaacttggct actacacgtt
    22321 cattggaata actctgtttt tcaggttgat tcggataaaa agcctgacgt agttccttta
    22381 aataattttc ttgtggcacc accttcaaca cttctccatc ttctgagagt acacgttgga
    22441 attcttggta atgagaaggc gaaaagatat ttaaaaccgc gttgaatgtt tgatcagcaa
    22501 acggcaaatt ggtcaaatca gctacacacc aaaatgcttc ggcagattgt tctgtagctg
    22561 cgtagactcc ttcttttgaa atatcaaatc caacagcatt ttgaatcttt ttcaattttg
    22621 aaagttcatt caagaagcta ccttctccgc agccaacatc taatagcgag acttcttcag
    22681 gtaaatgctg cgcaatagtc tcaataacgg gctgatacat gccagattgg atcattctac
    22741 gccttggaac gaacattcct tgatcgtatt ctgttttaac cgcatgggat aaaaaataaa
    22801 gtgttccttt tttggaaagg tcaaaacggt gattattttt gcatatcaag ccattatcag
    22861 acttttttac ttcttcttgg caaaccggac agcggaacag ctgatgattt tctgccaaaa
    22921 actggcgtcc tcggtcaatt ttttttaaca tagttccccc tcatttctgt tttatcttac
    22981 cgttagatgc tcactttggc aactgctaaa aatatgatac aatgcctaga agaaagtgaa
    23041 agataaagga gaagattaga tgatcaaaga attttgcgct gaaaactaca catctattcc
    23101 tctagcaatt gctagaggag ccgatcggat cgaattgtgt gacaacttag cggtcggtgg
    23161 cacaaccccg agtactggtg tgatcgaaga aacgttaagt tatgctgggg aaaaagatat
    23221 ccccgtaatg acgattattc gtcctagagg gggcgacttc gtctataacg atatcgaatt
    23281 gaaaatcatg catactgatt tgattgaagc aaagaaatta gggacggatg gcgtagtatt
    23341 tggttgtctt actccttcag gctggctaga cgaagaggcc ttagaactat tgatcgaaac
    23401 agcagaaggg ttgcagatca ccttccatat ggcttttgac agtattcctg aagatcggca
    23461 attcgaagca atcgattggc tggtagaaca tggtgttgac cgtatcttga ctcatggcgg
    23521 tcctgctgac aaaactattg tagaaaattt cccacggctt aaagaattga ttgcctatac
    23581 agatggccgc ttaatcattt tgcctggtgg tggtgtcact gcagagaatg cagcacatgt
    23641 agctaaagaa ttgaacgtaa gcgaagtaca cggaaccaaa atcgtggatc ttcagttata
    23701 atcttataaa caaaaaaaca tttgcaacag gcataatgct tattgcaaat gtttttgttt
    23761 ttttacataa aatagcccat aaataaagaa gcaatattga aataaatcag tacactcgtc
    23821 aaatcgctca aagtcgtgat aaagggaccg cttgctactg ctggatcaaa tcctagacga
    23881 tccattagca tcgggattaa actacctgca agattcgcca ctgtgattgc aaatagcata
    23941 gccatcccta tgacaaaccc caagataaaa ttatgtttcc aaaaacctac gattagaaaa
    24001 atcgtgatcc ccgtaacagc tcctgtgaca acgccggtca agacttcagc caaaacaagt
    24061 cgaacaaatc catgatcttt ctcatcagaa atcgcaagtc gacgaacagc cacagctagc
    24121 gactgggttc cagcattacc ggcagttcct gtaatcagcg aaatgaagac tgctaaaata
    24181 ctcgcttcac ttaccaagtc ttcatagtga ctgattaaag aagcggtcga catacctaaa
    24241 aacaacagag tgatcagcca aggcaaacgt cgagacgctg cctttactgg attttcatta
    24301 atttcttcca cattgacccc ggcaagtcct gaataatcgc tagcagcttc atcatcaata
    24361 acgtcgatga tatcatcgac tgtgacgatt cctaacaaat gatcatcata atcggtaaca
    24421 ggaagagcta agaagtcata atctctaaat gtttgtgcca catcttcttg gtcgtcgcct
    24481 acatgaactg aaagcactcg ttcgctcatc agatcagaaa tcattgcgtc gtcatcattc
    24541 acgattaggt cgcgcagaga aataacccct actagctgac cttcttgatt aacgacatag
    24601 atgtaataga ttgtttcagc tacatctgct tcgttcttca gcacgtacat cgcagagcga
    24661 acagtttgat tggctacgat cgaaacaaat tcagttgtca tgatcgcgcc agccgtttca
    24721 tcctcgtaat ggagaagttc cttgatttca ctggcatcgt ctgccgacat caaacttaaa
    24781 tatttagcaa tttgcttttt atccagcgta ttaagcaagt caacagcatt atctgtatac
    24841 atctcagaaa gcatatcagc tgcatagcta ggacgcattt catctaggta ttctttcata
    24901 tgttcgttgt cttcttcaat cacatcgaac atatccgcta attctttagg cgacagatag
    24961 gaatagatca gctgcctgtt ctcttctgta agagattggt agaattgccc ctgctcatag
    25021 atatgcatct ctaaaaaact gtttctgaat gcctgaatat tattttccct taaatcttga
    25081 gtcaactggg caaatctttc ctctaattct tgattttcat ccaaaccaat ctcacctcgc
    25141 cttattcttc aaaccgtctc acaactgcct ccatgtctgc ggctaaaggc tgcttgaact
    25201 ccagcaactc ttctgtaaat ggatggtaaa aacgcagatc ataacaatga agggcttgtc
    25261 tttttattcc cagatccatt cgcccgccat acatatcatc accgagcagg gcacagccaa
    25321 ttgcggaaaa atgaactctt atttgatgtg tccttcctgt atgcagctgg atatcaacta
    25381 acgcgatttc tgttagtcgc ttctctatcc agtactcagt ctgtgccgaa cgtccttcat
    25441 cagagaccat tcgcttcaat agcgaagaat aatctcgggc gatcggcaaa tcgatatgtc
    25501 catgtgccga taaatctgaa agccttccac tgactaaggc ctgatatttt ttcacaaact
    25561 tcttttcacg aagttgcata tctaattttg cgtgcgcaaa tccatgcttt gcaaaaagca
    25621 tcaagccaga tgtatcccga tctagtcgcg tcaccacatg gattacttgg ttttcgtaac
    25681 cttgtttttt ataataagcc tttacacgat tagccattgt cccatttgga tggtattggg
    25741 caggaataga cgaaacgcct gctggtttat ttactaccag aacgtgttca tcttcataca
    25801 cgatatcgat cggtgtttca tccaaaagta cggtttcatg ttctccttca gcaggaatca
    25861 caatggttac cttatcatcc ttagctagag aaaacaagac attttctact tgatcattaa
    25921 ctttgatttg accgccttga aattttattt tcgctaacag accctttgag atcccttttt
    25981 cttttaaaaa atattttact tgctgagaat gatctttctc ataatcccat gtaaactgca
    26041 tatcaatcct cactaataaa ggcatctttc acacgatgcc aaaaatgcat atgtcgataa
    26101 gaagcaaaat ggatccgttc ttctgctatt cgataataaa ttgagcggat atttgctttt
    26161 gcgacattca attgatcaac cgttacaaga taatcttctg tattttccaa ttttatttct
    26221 agccattcat catgggcaat cacgatcgga gaacccaaag tacgaaacac tcgattattc
    26281 agtgaagcaa tctctgccaa ctgaaaggca ttgatactag gatggatcac agcaccgccg
    26341 acacttttat tatatgctgt cgaaccagta ggcgtagaaa tcgtcaatcc atctcctcta
    26401 aaactttcaa acagctcgtt tttgatgtat acatcggcta ccatcgtacg attggcccgt
    26461 ttaatcgttg attcattcag tgctaaaaaa tgctggtccg gtgtttcatc caaataactg
    26521 atccgaacat ccagcaaggg ataactaaca ctttgttccc ggttcgttcg taagctgtct
    26581 actagttctt ccagctcgta atcccgccaa tccgtgtaaa accctaaatg acctgtatgc
    26641 acgcccaaga aacgcacatc attcagacaa tgactgtagt gatggaacgc cgacaataat
    26701 gtgccgtctc cgccgactga gatgaccaat tctggctcat gatcatctcg ttgaatccct
    26761 gcttgttcta aaagtgcgtt caaacgttct gtcacttcta tagtctttgc ttctttatta
    26821 tgaacgatcg ctactttcat cccctgcctc ctctctttcg ctttctttct tcaaaagcgt
    26881 tcgatagtag acatcatctg attttcctct tccatgggag aataaccgct gtgcttcttg
    26941 gatttcttca cggatactgg acatctcttc atccaactga taggcagctt ctgccgcacg
    27001 ctgaagtcgt tcactcattt cttctggaaa aacgccttga tatttataat tcagcgaatg
    27061 ctcaatcgtt gcccagaaat tcatcgcaag agtccgaatc tggatttccg ccaaaatttt
    27121 cttctcaccg ctgatcagct gtacaggata ctcgatcacc agatgataag aacggtagcc
    27181 acttgctttt ttatttgtga tgtaatctcg ttcttggaga actttcatgt cttttctttt
    27241 acggatgatc tcaacaactt gatggatatc ttctacaaac tgacacatga tccttaaccc
    27301 ggcaatatca gacatttctt cttctaaacg tcccattgga atatgccgta attgtgattt
    27361 tgttacaata ctatcgactg gcttgactct cccagtgaca aattcaatcg ggacatgtcg
    27421 attttgttca cggaactgtt tgcggatccc ccgcagtttc actttcaatt ctgatacagt
    27481 ttgttcatat ggtgctaaaa atgcttccca atctcgttcc atagctgata cctctacttc
    27541 attaacattc attgtttatt ctaccatata tgcaaagttt tttcgtactc ttgatttatg
    27601 cattgaccaa aaaaagaaaa ttaggcaaaa tagacaaaaa ggagtcgatc aaatgacaca
    27661 aatgcttgaa atcgaatata aatctatgct gacaaagcaa gagtacgaga tattcatcgc
    27721 tcactatcaa ttaacagata aagatttccg tgtacagact aatatttatt tcgataccgt
    27781 tgatgaacag ctgaagaaac aaaattgtgg cctacggatc cgtttcgttg gtcatcaagc
    27841 tgaatataca ttaaaaacac ctgctgaaaa agggcgtctc gaaacaacgg atacctttat
    27901 aacagaagaa gcagaagcat ttatcttcga aaaacgtctg cctcgttcag gcgcagtttt
    27961 tcaaaaactt tctgatttga atatcgatcc tgcttcatta atccaaaccg gaaagcttac
    28021 aacaaaacga gctgaattcc ctattgaaga aggactttta gctattgatg aaagctgggg
    28081 agacaatcta cacgattttg aactggaact agaggtaggg gatgcatctg tgggaaaaat
    28141 tgcttttata aattttttaa aacgtttttc tttaccttat cgtcctgcta agaacaagat
    28201 ccaacgtatg cttgaagcta aaaattagaa acctttcatt ttcacaaggc acttgagttc
    28261 atttttttac ttttttctgt taagttatag gatgtgaaag aatctagtca ctagttgtat
    28321 agaatctatt ttgggggaga ttcatatgat cgaaatctat ttatttgtta atcctttagg
    28381 ggaatactgt ttcgaaatgg aacaacagtt attacaattt atcgaagaag agtacgggac
    28441 accatcaaaa gaaaagatgc aatttcgctt tcttcctttg gtcaatttgc agacaattgg
    28501 ggatgtcatg caacaaaaag gaatttctca gaacaatctt gaggaacgta atcacttatt
    28561 ctcaatgact tattctgcag cattggactg caaagcggcg cagtttcaag gaaagaaaaa
    28621 aggccgccgt tttttgatca aattgcaaga agcagtcggc tgtaatgaca taccatattc
    28681 tcaagaactt gctgaatcca tatttgaaga aatcggcggc gatttgaata tgtttaaaga
    28741 agatcgtcag tctgacttcg ttaaagaagt attcttttct gatcaaaaaa ttgctcggga
    28801 aatgggcatc acaaaacacc cttctgcagt cgtttacaat tatgcctgcg accgtgattt
    28861 tggtgtttta gtagaagtaa aaggtacaag tagcctttgg agaatcacag agctgtgtaa
    28921 aacaacagat aactcatatc aagtttttaa tggtgaacat ttacatacaa atgattctca
    28981 gttccattca agaactagcc atttacactt gctttctaat taaaaa
//
LOCUS       DAGCWU010000018        28034 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.018, whole genome shotgun sequence.
ACCESSION   DAGCWU010000018 DAGCWU010000000
VERSION     DAGCWU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.018"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(32..796)
                     /locus_tag="MBZ71_000653"
     CDS             complement(32..796)
                     /locus_tag="MBZ71_000653"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBT4428629.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(797..1744)
                     /gene="prmA"
                     /locus_tag="MBZ71_000654"
     CDS             complement(797..1744)
                     /gene="prmA"
                     /locus_tag="MBZ71_000654"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBT4428630.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(1757..2245)
                     /locus_tag="MBZ71_000655"
     CDS             complement(1757..2245)
                     /locus_tag="MBZ71_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBT4428631.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(2211..2882)
                     /locus_tag="MBZ71_000656"
     CDS             complement(2211..2882)
                     /locus_tag="MBZ71_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBT4428632.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            3020..4306
                     /locus_tag="MBZ71_000657"
     CDS             3020..4306
                     /locus_tag="MBZ71_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBT4428633.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            4378..4572
                     /gene="ssrS"
                     /locus_tag="MBZ71_000658"
     ncRNA           4378..4572
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="MBZ71_000658"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            4650..4934
                     /locus_tag="MBZ71_000659"
     CDS             4650..4934
                     /locus_tag="MBZ71_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428634.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            5007..5486
                     /locus_tag="MBZ71_000660"
     CDS             5007..5486
                     /locus_tag="MBZ71_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4428635.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            5709..5852
                     /locus_tag="MBZ71_000661"
     CDS             5709..5852
                     /locus_tag="MBZ71_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBT4428636.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            6430..8013
                     /locus_tag="MBZ71_000662"
     CDS             6430..8013
                     /locus_tag="MBZ71_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4428637.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPINHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(8450..9634)
                     /locus_tag="MBZ71_000663"
     CDS             complement(8450..9634)
                     /locus_tag="MBZ71_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBT4428638.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(9659..10666)
                     /locus_tag="MBZ71_000664"
     CDS             complement(9659..10666)
                     /locus_tag="MBZ71_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4428639.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(10838..11197)
                     /locus_tag="MBZ71_000665"
     CDS             complement(10838..11197)
                     /locus_tag="MBZ71_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428640.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(11169..11540)
                     /locus_tag="MBZ71_000666"
     CDS             complement(11169..11540)
                     /locus_tag="MBZ71_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBT4428641.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(11557..11859)
                     /locus_tag="MBZ71_000667"
     CDS             complement(11557..11859)
                     /locus_tag="MBZ71_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428642.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(11876..12346)
                     /locus_tag="MBZ71_000668"
     CDS             complement(11876..12346)
                     /locus_tag="MBZ71_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBT4428643.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(12321..12599)
                     /locus_tag="MBZ71_000669"
     CDS             complement(12321..12599)
                     /locus_tag="MBZ71_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBT4428644.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(12596..13645)
                     /locus_tag="MBZ71_000670"
     CDS             complement(12596..13645)
                     /locus_tag="MBZ71_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBT4428645.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(13602..14531)
                     /gene="tadA"
                     /locus_tag="MBZ71_000671"
     CDS             complement(13602..14531)
                     /gene="tadA"
                     /locus_tag="MBZ71_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBT4428646.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14663..16417
                     /gene="ade"
                     /locus_tag="MBZ71_000672"
     CDS             14663..16417
                     /gene="ade"
                     /locus_tag="MBZ71_000672"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBT4428647.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(16515..17177)
                     /gene="trmB"
                     /locus_tag="MBZ71_000673"
     CDS             complement(16515..17177)
                     /gene="trmB"
                     /locus_tag="MBZ71_000673"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBT4428648.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(17247..18029)
                     /locus_tag="MBZ71_000674"
     CDS             complement(17247..18029)
                     /locus_tag="MBZ71_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBT4428649.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(18233..19444)
                     /locus_tag="MBZ71_000675"
     CDS             complement(18233..19444)
                     /locus_tag="MBZ71_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428650.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(19437..20174)
                     /locus_tag="MBZ71_000676"
     CDS             complement(19437..20174)
                     /locus_tag="MBZ71_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428651.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            20312..20746
                     /locus_tag="MBZ71_000677"
     CDS             20312..20746
                     /locus_tag="MBZ71_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBT4428652.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            20739..21083
                     /locus_tag="MBZ71_000678"
     CDS             20739..21083
                     /locus_tag="MBZ71_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4428653.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            21263..22273
                     /locus_tag="MBZ71_000679"
     CDS             21263..22273
                     /locus_tag="MBZ71_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4428654.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(22342..23286)
                     /locus_tag="MBZ71_000680"
     CDS             complement(22342..23286)
                     /locus_tag="MBZ71_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4428655.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(23270..25990)
                     /locus_tag="MBZ71_000681"
     CDS             complement(23270..25990)
                     /locus_tag="MBZ71_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4428656.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(25987..27207)
                     /locus_tag="MBZ71_000682"
     CDS             complement(25987..27207)
                     /locus_tag="MBZ71_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBT4428657.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(27488..>28034)
                     /locus_tag="MBZ71_000683"
     CDS             complement(27488..>28034)
                     /locus_tag="MBZ71_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBT4428658.1"
                     /translation="KIYTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSI
                     CSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIV
                     PQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKAD
                     YVTVSNDKDGVSQAFERILTI"
BASE COUNT         8473 a         5741 c         4891 g         8929 t
ORIGIN      
        1 tctgtcgata ctcttttatt aatgaagaac gttagctgaa agaaggattc attaattccc
       61 attgatagct agcagcactt aataaataaa gaggtgctgt ttctgtacga aggattcttg
      121 gtcctaatcc acaaagtatc cctttgtgtt ctacaaaata atcgacttca tcgcgggata
      181 atccaccttc tggtccaaag acagctaaga ttcgactatc tggttttgtt tcactaagaa
      241 ttttggctgc atttgatttt tcgccttgct ttgctgattc ctcataagca accaagacca
      301 cgtcataatc atctagccgc tcaaataaat cttgtttcgt ttccaataac tctactgatg
      361 gcacgatctg ccgatgggat tgttccgctg cctcttgagc aatcttttca agccgttgtt
      421 gtcgtttcat acgttttttt gcatcccatt taacgacaga ggctgatgcc gggaaaccaa
      481 gaaaagcttg cgctcctaat tcagtggatt tctgaacgat ccaatccagc ttttcccctt
      541 taggatagcc actcgctatg gtaatcgaac ttggcagttc cttttgattt tcttcttttt
      601 taatttcttt cagataaaca gcctcttcag taatctccgt gatttctgca acaatggcta
      661 tttgatcatt gaatgccaaa aaaacttcat cctcgggttc catcctcata acccgtacaa
      721 tgtggtgata gttttcccct tctaaaacaa aatagtcttt tgattcatat gattctttta
      781 aaaaataccg ctgcatctat tccacctctg gttttttcaa aataattgca taccagtctt
      841 tttgctggaa aatttgatcc acgataaacc cttgttctaa catggcatcc agaaccattt
      901 gcttcttctc ttcaatgatt cctgaaacga tcagcgtgcc tgtttgcttc agtaaacgcc
      961 aagcatctgg gatcatcaat aagatgatat ctgctaaaat gttcgcaacg attacatcac
     1021 tttcaatctc gattcctgtc aaaagatcgt tagctgatac atggacatct ttggctattt
     1081 cattcatatc catattttct ttcgccgaac gaacggctac ttcgtctaaa tcaaaggcat
     1141 agacatcttt tgcaccaaga tattttgctg caatacttag cacgcccgac ccagttccca
     1201 catctagcaa tgtttcacca ccacgcaaga ctgtttctag tgctgtcaat gtcaaggaag
     1261 tcgtgggatg agtgcctgtg ccaaatgcca taccaggatc tagtgtgata attttttcta
     1321 gaggatcttc tgtctggtag ttttcccagc ttggcgcaac cgtcagcatt cgcgtaatac
     1381 gcaccggatg atagtatttc ttccaggctg ttgcccagtt gctttcctct acttcactaa
     1441 ctgttacttt attttttcca atagctagac caaactcagg taattgtgtg atacgatctt
     1501 tgataaaagg caagatttcc ggtaagaaag tcgtttccgg aaaataagcc atgactagtg
     1561 cgccttcttt cagcgatgta aaatcatttt tatctaaaat ctcaccatat tgatccgact
     1621 gaaaatgttc aacatctaaa gaatcttcaa tcgcgacacc actcgcccct gcctccatca
     1681 aaatattagc aactgcttcg actgcttcac tttcagtttc tacttttact tcattccatt
     1741 tcatatctgt cactccttaa taacttgggt acggtaaata agtatctgct tcattttgct
     1801 gttttacttc tactaattct gctagttgct gattatccca gtgaagctcg aatacagctt
     1861 cgtcatcgtc ttcatccaaa aaatcaagca aatcgctttg cccttgagct aatacttcat
     1921 ttagatattc cactaaagaa tcagctactg attttgccaa cccttttttt ccttcataag
     1981 ggattactgc tagataattg cgttcatcaa aaacagattt ctgaggatta aaaagaagta
     2041 cgccgtcttc aaattcgata attggttctt gcgaaacgat cccttctgca tcatcaatct
     2101 caaattgtgc tttattttct gcaaacaagc gaacaacgat ctcgatcgta tgattttttg
     2161 tatcccaatc taaagctaca tcatagtctg tcacttgttt ttcaattatt tcatctaaat
     2221 atgttaatag atccttcttt ttcattttct ttcctccaac cccaatcttt ctcgatttgg
     2281 ttttttcttt gtaaagagat ataaggattt cccgacacaa caaaacgcaa cggcttttct
     2341 gtccatacac ctttgtttgg gataccgatt ctaggcaaag cattgatctt ttttggtgtc
     2401 ttttttttct caaaaactaa gcgtaacgaa ctatctgcta tagactgacc ataaagttct
     2461 tgaggaattg ctaatgcttc cactaatttt cccggaccgt tgctgatatc tggaccagtt
     2521 tttccgccac gatttttact catttgatca atcattgcgg cgggttcgat tgcacgtatc
     2581 attactcctt gaggaatccc ttctggctga gtaatgatgt tcaaaatccg atgagtatgc
     2641 atcgtgtaaa ggtagatcgt acctggtttt tcatacattg cccgcacacg cggtgttctt
     2701 cttaaaccat agctatgggc ggcctcgtct tctggaccta ggtatgcttc cgcgtctaca
     2761 atatatccag ctaatcttcc ttctggtgtg tcatgctcca aatacatccc caataaatcc
     2821 cgtgccactt ctggcgtcgt tttattccaa aaaaattcat ctatcttttg tttatccgtc
     2881 atttttctca cctactttat catacacaat attagttgga taagctatga aaacaaaaca
     2941 acagaaggct gttctttcta ggcatcttgc taaatatgat ataattaagg gacgtaaaaa
     3001 caagaaggag gaaaatccta tgcaacagcc attggcttat cgcatgagac ctcgaacgat
     3061 cgatgaagtc gttggtcagc agcatctagt cggggaagga aaaatcatac gccgcatggt
     3121 cgatgctcgc atgctctctt ccatgattct atatggacct cctggtactg gaaaaacgag
     3181 tatcgcaagt gcgatcgccg gctctactaa ttatgcattt cgcatgctaa acgccgctac
     3241 agattcaaaa aaggatctac aagttgtagc ggaagaagca aaaatgagtg gtacagtgat
     3301 cctcttattg gatgaagtac accgattaga taaaacaaaa caggattttc tgcttcctca
     3361 tttagaaagt ggccgaatca tcttgatcgg agcgactact gagaatcctt atattaccat
     3421 caatccagcg atccgcagcc gtacacagat ctttgaagtc aaaccattaa cagaacaaga
     3481 catccagttg gcagtcgaac atgctttaag ggataaagaa cgcggccttg gacaacaagc
     3541 aatccagtta gacgaagatg ctcttcttca cctttcgcgt gcaacgaacg gcgatttgcg
     3601 gagtgcattg aatgggctgg aactggctac actatcgact ccttcagata aagaaggaag
     3661 gattcatctt acactgtcga tcattgaaga atgtgtccaa agaaaagctc ttacccatga
     3721 caaaaatgga gatgctcatt atgacgttat ttcggctttt caaaaatcga ttcgtggaag
     3781 tgatgtggat gctgcacttc attatctcgc ccgtttagtt gaggcaggtg atctggcaag
     3841 tatttgccgg cgtctgatgg ttatcggtta tgaagatata ggtttaggta atcctgctgc
     3901 tgcggctcgt acagtgaacg ctgtattagc tgctgaaaaa ttgggattgc ctgaggcacg
     3961 tatcccacta gcagacgtcg tcgtcgatct ttgcttgtcg ccaaaatcca attctgccta
     4021 catggcatta gatgcagcac ttgctgatat tcgagagggc aaagctggag atgttcctga
     4081 tcatttgaga gatagccatt acaaaggtgc gaaatcactc aaccgtggcg tgggttatca
     4141 gtatcctcat cattttgatc aggcttgggt caatcagcaa tatttaccag ataaactgaa
     4201 gaatgctcag tattatcagc caaaagatac agggaaatat gaacaggctc tgggtcaaca
     4261 gtattaccgt ataaaagagt ggaaaaagca tcctccaaaa aactagacaa gaaaacggtt
     4321 ctgtttctgc gagctctctc attgctcttt ttaaattcat atgctactat agaggtggga
     4381 aatctgtgat gttcgaattg tcctctttgt gtgttgaccg aacattttta ttgatatctt
     4441 gggacccgtc ttgtgtcact gctggtaaca tgccttatca tttggtagtt cgaagctacg
     4501 accatgggac ccacctgctt gaattcgcgg gatcaatact acaggacaaa caacggcatt
     4561 aacggagatt accatctttt aaagaataaa gcaatttgct cttggcaaat tgctttttct
     4621 atttgttgct tattttggag gatttgttca tgattaggat tttaatgatt atcgccactt
     4681 tagtattatt attcgttagc tattatttat ttaataaaca ggatatcttt tttgtattga
     4741 tcaacaaaaa tgataaaaat caaggattcc tacaatttta cggagctgca tatgctgttt
     4801 taggcgtaat gggtatttta acagcttttt tcaatcaacg ctttattgcc ttggtctttt
     4861 tactgatcgt aattattgtt tctgccacct tcagtatccg atttgcaaaa aaaattgctg
     4921 aaccaaagca gtaaataatt ctctttttcc ctaaagtgtt ctacaataaa agagtacaat
     4981 acgattgact tgaggagtga atgcttatgt tacaacaata caaaaaaatc atggttgccg
     5041 tagatggttc tgatgaagct gaattagctt ttaaaaaggc ggtcaatgta gctatccgaa
     5101 ataacggaga acttctatta gcacatgtca ttgacacacg ttctttccaa accgtttcat
     5161 cctttgatgg aatgcttgca gaacaagcta ctgaaatggc aaagcaaaca ttggcagatt
     5221 atgaaagcaa tgcgaagaaa gctggcttga ataatgtaac ctctgtcgta gaatatggct
     5281 caccaaaaca aattattgcc agagaaatcc cagaagataa tcaagtagat ttgattatgt
     5341 taggagccac aggattgaat gctgtagaac gtttatttat tggttctgtt tctgaatatg
     5401 ttattagaaa cgcagcttgc gatgtgcttg ttgtccgaac tgatttagaa aaccaattgc
     5461 cagctaaaga ccacaccaaa gcttaacacg tagacgacct gccaactatc ggcaggtttt
     5521 tttgcacaaa aaaccgtgat aaaaacaaaa gacgcggcaa gcactcatcg gtctttataa
     5581 tgtttaaaat aaagggaaaa cagtcaatga gttcaagtaa aataaaaaaa agtgaacacc
     5641 agggcgatta gtcgccctgg tgtggtttaa aacgacctaa gcatgccaaa gcttattggt
     5701 tcgtcaatgt ggaagtttcc gatgaatctg atacgaaaaa atatgaagta tcgatggcgg
     5761 ataccgattt tatcgccaaa ctaaaatttt atttcgggtt cgggtaccgc gagaaggcaa
     5821 gtgctacatt cggtaaatca ttcaagaatt gatgaaaggc taccgcttgc cattgcaacc
     5881 acaacgatac tcctgaacgc gatgttggtg agatttccaa agagaagagc tgtttaatgc
     5941 aacggtatta agcaaatggc aaaaggaagt catgtagaca aagtttttta tcaaacacca
     6001 tatgatttcg cctgaacgct ggtttagctc agaatacaat gatgtggtcc acgagacatt
     6061 accctcagtc agataaaaaa agctctctga cagaattaca catcgataaa tattaagtaa
     6121 atgtcagcgc tagttgaact ttctgacact ttcaggtaca ctggaaaaga caactacacg
     6181 aaggagggaa aatactatga tgcgcatgac taaaataaca agtaatgaaa attaccaacg
     6241 ataccatatc gcatttagcc atgcaattgg ttttacagtt gctgttgacg ggacaagttt
     6301 gtccaaaaca gttgaattca cgtaaaatca atggccatct caaggtattt ttctcatgat
     6361 aatctgagtt taaataacac aggccatgga taaccatggc ctttttgagt ggtctcaagg
     6421 agagagatca tgatcataca agcaatttct attaaaaaaa caatcggcgg gaatccatta
     6481 tttgaggatt tatcctgtca aattgaagct ggtgaaaaaa ttgccttagt cggtatcaac
     6541 ggaactggca aatcaacctt tttacaaatc ttgaccggca gagaaggtgt cgacagcggt
     6601 gttatcagtc gcaaaaaagg cttaaagatc gggacggtcg aacaagaact tacagtttcc
     6661 gaagcgaccg tcaaccacta cttgctccat tctgccgctg agatacagga cctcaaacag
     6721 caaatgagtc gctacgaagc attgatgaca gaaccagata caaacttaga gaagtgtctg
     6781 gcccgttacg gtgagctgca gcatcgcttt gaagaactag gcggttacgt attagaagac
     6841 cggattgcag cgatcttgca aggcttaggg attccacacc gtcaggaagc acggttaaca
     6901 gaacttagcg gaggcgaacg agttaaagtc gctttagcca aaattttagc agccgatgcc
     6961 gatttacttt tactcgatga accgatcaat catttagatc ttaacagtat tcgctggttg
     7021 gaaaactatt tacagaatac aaaaaaatcg gttctggtcg tttctcatga tcggcaattt
     7081 ctcgatcaag tcaccacaaa aacatgggaa ctggaagaag gtgcattgat tgagtatccc
     7141 ggaaactaca gccgttttcg agtcttgaaa gaagctcgct tggctgagct gacaaaaaat
     7201 tacgaactgc agcaaaaaga agtccagcgg ttgaaagtca tgattcgtcg ttttcgtcag
     7261 tgggcacatg aaggagacaa tgagtccttc ttcaaaaaag ccaaagaact ggaacgccgc
     7321 ctggcgaaac tgacactcgt caaaccgcca cctcccccga aaaatcggct gcaaagcttg
     7381 tctaatggtg gaaaatctgg caaagaagtt ttcatcattc aaaacttgca ccagcagtac
     7441 gctgatcaag tactattcaa agacagcagt tttgcagtct atcgtggaga tcatttagcc
     7501 atcatcggcg acaatggtgc aggaaaatca accttactca aattattact cggtgaggaa
     7561 aagccttacg gtggcaccat caaattaggt agtagcttgc aaatcggtta tttgccgcag
     7621 cagctgcagt ttgccgatcc cgatagccgt ttgctggctt acgccatcac attgactgga
     7681 aacgaagaag tcgctcgacg gcaattagcc aaaagtggct tttatcaaag cgatgtgggt
     7741 aaacggatca aagatttatc cggtggtgaa aaaatccgtt tggcgttgct gaaactcttt
     7801 ttagaaaaaa tcaacgtcct gattttggat gaaccaacca atcacctcga cagctatgct
     7861 cgagaagaaa ttgaagaaat gctgcaagac tatcaaggaa cgctcctagc agtcagccat
     7921 gaccgctact ttttgcagca acacttccaa gaagcactcg ttattgatca ggagcagatc
     7981 agccgccagc cgctcggcca actgattgac taggacgttt tttcgcaaca aaaaacttga
     8041 ttttcatacc tgtgctttca cgattcaggc aaagtacagg tggaaaatca agtttttatt
     8101 attcgatcaa tgaatgtttc ggatcggttt tctagctaaa cataaagcga agaataaagt
     8161 cgacagccca cgaggctgat ctaaaaatgt tttttctttt tacatatgta atgtccctta
     8221 taaagatgaa tgaaaataaa atattgggat tttcctgaaa atcaataaat aaaatgccag
     8281 tcttttccct tttgaccact tttccaagca aaattcccta agaagatcct gtaaaaaaga
     8341 agggaaattg aagttaaatt accattttgc ggtcttctcc gcaaaaaaaa cacatgcaag
     8401 aattccctct tgcatgcgtt tcatctttta tcctaacgaa tgctttggct tagccgcgca
     8461 gacgctccac atcacgggca atcattagtt cctcatctgt tgggatcact aatactctta
     8521 cttttgattc ttttgtagag atatctactt cttcaccacg caattcattc aacgctggat
     8581 cgatttcaca accaaaccaa gacatacctt tgatgatttc actacgtaca tgtccgtcat
     8641 tctcgccgat accagcagta aagacaattg catcaacacc attcatcgtt gtcacataac
     8701 cgccgatgta ttttcggatg cggtccacga aaatgtcata agctaaacga atatgttctt
     8761 tttcatagtt cttttcaaga tcacgcatat cactagacaa tcctgttagg cctagcagac
     8821 cagatttctt gttcaaaaca ttgatcattt catcaatatc aatctctaat ttttccatta
     8881 aatatgggag aacagctgga tcgatatcac ctgaacgtgt acccattgtc accccagcta
     8941 atggtgtgaa tcccatagaa gtatccatgg atttacctcc atcgactgct gtgatcgata
     9001 caccatttcc taagtggcaa gtgatgattt ttagttcttc gatcggtttg ttcaataatt
     9061 ttgccgcacg ttccgacaca taacgatgac ttgtgccatg gaagccatat tttcttactt
     9121 tgaaatcttc ataataagac accggtaagc tatataaata attttgcttt ggcattgttg
     9181 agtggaaaga tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc tttttaaacg
     9241 ctttgatccc cattaagttt gcaggattat gcaaaggagc aaattctgct aattcagcaa
     9301 tgcggtccat tactttctca tcgatcacta ctgaatcccc ataagtttct cctccgtgaa
     9361 caacacggtg ccctactccg gtgatttcat cataagagct taaaatttgc agttcaatca
     9421 actgatctaa cagcattttt actgctactt cgtggttttc gatatccatg atttgttcga
     9481 atttttgatt atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg atacgttcga
     9541 cgatcccttt cgcaactacg gtttcttctg gcatttgata taattgccat ttcaaacttg
     9601 aactcccagc attgattgca attgttttag acatatgact ctccttctcg ttgaatgatt
     9661 acagagcaga tgatttccag tttttaaact cgttgaaaaa ttccgtcacc ttctctggtt
     9721 cttttaatga agcaagtttc acaagcaaga cttcttttac ttgttgtgct tgtggtcctt
     9781 ttttctgtaa gatcaaaata cttttttgcg attgtttatt acggaacaat tcatctggca
     9841 attgaatcat tccttgaaga taaccttctt ctttaaacca ttttttgatt tcttcacttt
     9901 gatccgtttc taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc ggcttcacat
     9961 atttcattga ttgctctagc aataaatgat gtgcataact atggccttct tcggtacttg
    10021 ttaaaaactc ttttgctttt tcgtcattcg gataataacc gattggcaaa tcgctgattg
    10081 caaaatcgac aggatctatt aataattcct gcaacccgtc ttgatgaaag tattgaacat
    10141 ttgcttgtgt caaatcactt gtagaagctg cgacagccaa caatgtatca tcaatatcca
    10201 caccaattcc ttctgtttgg taccccgcat tcgataggtt taaaagaact gttaataaca
    10261 agttgcccat tcctgccgca atatccagaa tcttaaccgg tgctttttta tttgtgtata
    10321 gttgttcaat aagaaaaaca aataaaaaac cgatactatc aggagtgagt tgatggttcg
    10381 cctgcaaatg ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt ctgcgccatt
    10441 cttctggctc gaaagaaagt ttcttcaact cttcataaag agctgtgatc cgttgcgttg
    10501 tctcttttgt cggcactcca tccaccacac gtacttgata atcatctatt aaattttctg
    10561 cattttcgac ataggcttct aaaaaggaag ttcccaatgc attctgtaaa agctgaatag
    10621 cttccaggtt ttgattaaac ccttgttcca ttttttctgg taacatggtg cacctcattt
    10681 ctagatatat tataacaatt ttttcacctg tttaattcta tcgaacaatt gacaaaattt
    10741 caatcgtttt cactgttttt tatttgtgaa acggcaaaca tttttttatt aaaaacagtg
    10801 ttataacaga aaagagggcg ttttcacgaa cattccatca ttctgtacta gtatctgtat
    10861 cattttttac ttcttctgtg aaccgaaaga taaacggtga gacttccacc tctaagaata
    10921 gggactgttg tacccactga tatgttacag atccagttgt gtagtaaaaa actccttccg
    10981 aactttcggc aagcgtagac tgttccgata aagccatctg cttcattatt tttgctatat
    11041 aataattttt gattcgtata ttaagatcca ttgacaaacg gtagctttct aatgctgcca
    11101 tcaataaaaa cgtacataaa agaaataaaa gcaagacaga aaataaaata gagcccttat
    11161 aacattttct actgatcgtt ttttgtccat atggcatatc catcttctcc attttcaaag
    11221 tgtacataaa aagaaattgt gtgttctttt tccaataatt gaagctcagt gatacttgtt
    11281 aacataggtt gatgtccgcc ggaagttctt cttttacgta tcattgcctg atatctttcg
    11341 atgatatact gtttatcctc gatcgtgtag tgcagttgct gatttttcac agagacattg
    11401 tgactgctct tgagttcttc ttctaattgt gctaaaaata ttagccactc tttttgatct
    11461 ttacgctgca aaaattcact tacttggatg gtttgctgaa tcaaaagatt gaatgtgagc
    11521 aatagactgc tcaatatcat cagtccaatt agacattcaa tcagcgtaaa tgctggaatt
    11581 acttgttttt tcaatactga tcgcttcttt ctccatattg acaaacactt tttcgattac
    11641 tccattatta tcgacaattt ggatatcgta tgggatcatt tcttcccttc gctctggtct
    11701 tttgtgatac atccgctgcg tcttgatatc ttcataaatc attcgaataa atagcagttc
    11761 ctccccactt ttcttttctg cctgaagcaa aaacagctgg ccaaagctca ctaagtagac
    11821 acagacagaa gcgatcataa gcgctgtaag ggactccaac aaaacaaatc ctgttttatt
    11881 caattctttt tgtatagcga ccactcccta tctggaattg atacgtaatc gtccgctctt
    11941 tttcttccca ataaaattga taagctttta gtgaactttc attcccggtt ctagcagcaa
    12001 aagtaataga agcatggcgt ttcaaggtaa tctcatcagg aatatctaat acttcccaat
    12061 ttattttatc tggattcgga acatgaaaaa tgatttgatt ctcttcacta ttccaataaa
    12121 agcctgtttg gatctgattc acaatagcga ttttttgcgt agaatagatt cgtttttcaa
    12181 attggccgaa aaattgatag actgccaatt cattcttcca tgcagaaaat gcgagaactg
    12241 gaaaagaaag gatcgttgta agtaataaaa tcagtaagag tgattcaaac aacgtatatc
    12301 ctgatgctaa aaccttacct tcaaggccgc tgatagattt ctgcatgttc tcgtttgata
    12361 tagccttcat tcaacaattc ctccacagta ggaactcttc cattattttg aagcgcatag
    12421 agctcttttt gtgtgtctac caactggatg atcgcttcac gactttcttg gtctacatga
    12481 ttcctataac gcgacaaatt agggacgaac aacaacacta atacactgat gatcagtaag
    12541 acgattagca tttcgatcaa tgtaaaaccg ctataataag atatttttct ctttttcatg
    12601 gcaaaacctc ctctatgctt ccgtaaacag gtaataaaac agcggcataa actaataaaa
    12661 tcaaagccgc tacgcccaaa aaaatgattg gctgaataaa atgcatccag tgatgaagac
    12721 gtttgaaaag ctgttttctt gtccattcgc tgtaaaaaat cagttcttta cctaaacttc
    12781 cctttgcctc tccctgtaaa acaattttac tgaattcttc tgatagaaaa cgataattct
    12841 ggaactgctc agccaatgac atacctgcct ccaatccttg aatcaactta gaggcaagat
    12901 gctgaatcag actttcttgt ttcgtttcct ttaagcagac tatgatttgc cttaattcga
    12961 aaccttcatg aaaaagcttt ccaatctcta gtgacaaata agccgattga tacaaggaat
    13021 atatcggtcc aatcaataat tgtctgctta tccattcact ctgatcaaca ggagacaact
    13081 tcatcagacg ataccttact agtaaagcca atagagtaac cgctaggatg aatagtagaa
    13141 cataccaatg aaagacttgt atcagctgaa taccccaatg ctcttgcatg accattcctg
    13201 aaccaataag ctgaggcaaa acgaacaacc gcatactaaa caagatacct agcaaaaagg
    13261 tgagcaatag catcggatat gcaagcagct ttttcaaatc attgcgaaat ccttcaacaa
    13321 gccgaaactg tttggcaatt tccaacaaag ttgtcggcag attcccatga cgttcagcta
    13381 actcgatctg taccagttgc tctgctccat atcctatttg cgaaagtacc tcactaaacg
    13441 cttgcccttc ctttagctga tgttggatat ttgccaaaat aacaggtgga aacgaacgga
    13501 tcgtgagtaa taaataaagg gattcttgca gactaaaacc agataaaagc agttctccca
    13561 tggtttgtgc aaactggttt tgctggcttc ttgttaatcc tttagaatag ttctgactgc
    13621 caaatatttt cctgagcttc ttcataactt tcctcccatg atttttttac tgattcttcc
    13681 tccataaatt gataactcca taaaacagaa ggatggtgat tggcatcttc caaaagttct
    13741 tgataaatga caccagctaa acattcagat agctcttctt ttttctttgt cagttcatgg
    13801 attctgacca atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac ccggtgccct
    13861 gttagtgctg ctcttaccgc agcatgtgca gttgattcat ctcttatctc gccaatgatt
    13921 agcaagtctg gacgatgccg taatgcgagt ctcaaaagtg tatcataagt ctgtgctatt
    13981 ttcggattga tctgtagctg cagaaaaaac ggttcttcta tctctacagg atcttcgata
    14041 gtaatcactt gcaatttttc ttccaaagcc aaccgataca ttagagaagt cttgcctgat
    14101 ccgactggtc cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa aatagttgca
    14161 aaatctcgag aactaaagca acgatggggt gtttcaaaag taccgtataa tagccgaatc
    14221 actaaactct ctcgttgaag gtaatctcct acagaagaca accgcagacg aacttcctgt
    14281 ttttctaaag aataagtaaa tgcacctaac tgagcttttc ttttttctcc gatatccatc
    14341 tgtcccaaat acttaaatct tgagatcagt tgctgtccat ctgccgaaga gactttcttc
    14401 cagaatgttc gtttttttcc atagcgaaaa tagatgtaaa atacttctga agctggcaat
    14461 atatatacgt cttgggcatg tttggcaaaa ctttcactta tcatttgatc cgataatcgt
    14521 tttatttcca ttattctccc ctccacaaaa aacatacgtc tttttgatct ttttgtttca
    14581 aataaaatga atttacaaaa aaatattggc aaaaaccgaa tttatgttat acttataaaa
    14641 atttatcttg ggaggcactc cattggaaac aacattaagg aaatatatcg aagtagcaag
    14701 taaaaaaaag cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg tctttacgaa
    14761 agaaatcatg gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa tcggtgaata
    14821 cgaaggaaaa agcgtacata atgctaatgg gcaatatcta gtgccaggat tcatcgatgg
    14881 ccatgtgcat atcgaaagct cacttttatc accaagcgaa ttttcaaaag tatccttgct
    14941 tcatggagtt actactgtag tgacagatcc ccatgaaata ggaaacattg ctgggagtac
    15001 aggtcttgag tttatgatag aagatgcaag acaaacactc atgaatatct tttttatgct
    15061 accttcttgt gtacctgtaa ccccttttga gaccaatggt gcaaatttaa atgcaaaaaa
    15121 attagcccct tttttacaaa aacccgaagt tttaggctta gctgaagtca tgaactatca
    15181 agcagtagca aataatgaaa cagacatcct agaaaagatc caattgatgc accagcataa
    15241 gaaaaaaatt gacgggcatg cagctggtat cggcatggaa gagttaaatg tttatcctgc
    15301 agcaggtatt cggacggatc atgaagctac aactgcaaaa gaagctaaag aacgtttgga
    15361 tcttggtatg tatttaatgg ttcgagaagg gaccgttgca aaagatctgg catcgctact
    15421 accggcgatc acacctgaaa attcaagaag atgcttcttt gtcaccgatg ataaactgat
    15481 caatgattta gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta ttgcacttgg
    15541 aatcgatcca ttacaagcca ttcagatggc aacattgaat gcagcagaat gttttgattt
    15601 gaaaacacta ggtgcgattg cccctgggta tcaagctgat ttcttcttaa cagacgatct
    15661 taccaagctg ccaattcatg aagttttcta taagggaaca ttagttgttc aatctgggca
    15721 actccaagac catttatttg ctgatcattc aaattcatat acgtgttgtc tgccaaaact
    15781 aaacgctcaa ccattaaatc cacattcgtt agagctgctt ctttcttctt cacaagccca
    15841 tgtgatcgaa attatcccga atagtctagt gactaaagat ttggtagaaa aagtggaccg
    15901 acaaggcgaa cgattcattc cttcaatcga aaaagaccaa ttaaagattg cggtgatcga
    15961 gcgtcaccat caaacgggaa acattggatt aggcatagtg aaaggatttc agctgaaaca
    16021 aggagcgata gcgacaacga ttgcccatga ctcgcataat ctagttgtcg ttgggacaaa
    16081 cgacgaggat atgctgtatg ctgctaatct tttgattcaa aaaggtggcg ggatgaccgt
    16141 tgtcaacaaa cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa tcatgacaca
    16201 ggaacctttt tcgatcgtct atgaacagtt gaatgaatta ataactaaag cacatcaact
    16261 aggagcagct aaaacctttg atcctttttt gacactttct ttcttaacat tatctgtcat
    16321 acccgaattg aagatcaccg acaaaggatt attttcattt tctcgcttcc aattgattcc
    16381 accttgtgga aaaaccgatg aaataaatac attttaaatc aaataaatcc cagaaaatac
    16441 attacatctg taatgttcat tttctgggat ttatttatgg atattaatgt ttttatgaaa
    16501 gcagaaagtt agtttcacag acttttcttt ttggtcttat cttgaaattg agcttctact
    16561 cgataaatac gttgaccttt tgaagaaaat ttttcttcat actccgtcat aatattgcct
    16621 tcaaattgac tttgatgtaa atctaaccat acctgtttga ggatcatgcc atattgagaa
    16681 aaacttgcca gagaatactc aaacaatcct tgattatctg ttttgaaatg gatttctcca
    16741 tttggacgaa gaatctgttc atctacagca agaaaagtct tatatgtcaa tcgccgtttt
    16801 tcatgacgtt tttttggcca tggatcggaa aaattcagat agatttgatc tacttcgctg
    16861 tcagcgaaat actctgtcaa cgctgatcca tccacatgta gaagtttcag gttaggaagc
    16921 ggttgttcga tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat ttctactcca
    16981 atataattga tttcaggatg ggcttttgcc attccagtga tgaactggcc tttccccata
    17041 ccaatctcaa tgtggattgg atgatcgttt tcaaacaatt cattccattt tcccttccac
    17101 aatgttggat cactaattac aaaattcggg tgagccgcta acatttccgc tgctcccgga
    17161 cgatttcgga cacgcattat ttatctccat tcctttcctc taattaaaaa ggtctggcca
    17221 aatctccgca gagtttgggc caaacctcag ccactgaaaa ttcgttttaa ttgtaaaatt
    17281 tcttcgttca tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc ttggagcaaa
    17341 ttcatggccg cataccaatg aaatttttca agcatttcgt cagtcgggcg tatgccataa
    17401 ctgatcagcc actgattcca gctagacagt ggcacataat ttcctaaaat cgttgcaata
    17461 tccaccgcag ggtctgcaaa catgattgaa tcccaatcga ctagatataa ataattttta
    17521 catactaacc aattacgatg gtttacatct ccgtgtacta ctgataagtt ttccttatct
    17581 attgacggga tctgctcacg aagaaaacga tggacaattt gcagaaattg atttttctgg
    17641 agttcttccg gtaattcttt ttcatatttt tgcagcatct tctccggcgt catgacacga
    17701 ccaccgatac gggtcagcat ggttttaaga gaatttgaat gatgtaaatg gtacaaaaca
    17761 tcaatcacgt cgtttctttg accaatctca ttaggagtca gcaagcgacc ttccagccac
    17821 tcttgtgctg tcaaagtatc gccattcccc gttcgttttg tccaaacaag ttttggtgta
    17881 attccttccc ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat aaagactttg
    17941 tcctcatcac gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg acgtaaccgc
    18001 cactcttggt ctaactgaaa ctccatcatg cattctcccg tcctcttact ctttgttttg
    18061 cagtacttat tattctagtc gcctgaacac tacacgtcaa gcacatatac gttctttttc
    18121 atttaattgc cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa cagcattctt
    18181 actgtcaacg ctctattttt cattctcatt tttaccctac acagctttgt tttcagtctg
    18241 acatcttctt caaacgagtt ggtacatata gtttaacaaa gagcatagta acaatcaaat
    18301 aagccgctag cggaacccat cgctgctgcc catctaatac gactgcagca atcaaaccaa
    18361 aaataaccga cgcgataatt aatacccatg tgagtagata ctgaatcgcc tgcttcttct
    18421 gattcttatg gattggataa agctgaacga gaaccatata gcggaactgg ttcgctaaag
    18481 gtaacagttg gaaaccgatc aaatataaaa ataagaaacc gataatcaga ctaaaataaa
    18541 agtcgactac tccggcaatc aataacccac ctataattac taatcggaaa taaagaccac
    18601 taaaatcaga acctcttgcc aatcttcttg caaacaagta aagataagtg ttttttgatt
    18661 ctcttttaat atgagtcaat ataggatcca aatagcgtct tcttttgatc tttgccgtaa
    18721 tttcaggaac atctgtgaat aaattgataa attgatagat ctgatgcaac ctatgttctt
    18781 ctacatgcac cattttctcc caatccaatc ctttttcttg ttgcttccac aaaaaccagt
    18841 aatatagact tgcctgtact aatgcacctg ctattcctgc ccatattagc caataaaatg
    18901 atagaaacaa aaagatcaga gaagaaaaaa accagcctaa tttccaataa cgaataactt
    18961 gttgatctag tcgaaacaat tctgctctct tgatttgcag atgactgaat ttcaaactcc
    19021 ataatatgat cagatagacg aaaaacatcg aaaaggattg ctccgtacta accaccacta
    19081 atggcatggc caatccgcat gccaatatca aaaagatcaa cggaaaaatg caagaatagc
    19141 gaaaagctcg ttccaaatag gtttgcattt ctttttcttt aggaagtaaa aaaacaatat
    19201 ctgctgcctc tgcaaaagta gccatcttac ccacgaataa acagagcatc caaaatacag
    19261 agattatgag ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa ttagaataat
    19321 ataatcccac accgccaatc aagaaaagac aaactaaaac aaaatgatcg ttcaatacat
    19381 aacgcatata tttcatcatt ttcttttgat gtcttgctaa acgtttttga aaaaaactac
    19441 tcatgtgcct gctcctttgt taaagcaata tagatttcgt ccaaagaaga atccggcagc
    19501 tgaaattctt ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc gttatgtaag
    19561 accacaaaac gatcacaata tttttctgca gtagctaaaa tatgcgtaga catcaaaata
    19621 ccggctcctt cttttttcat ttctaccatc aattcaagca atgcattgat agctagtgga
    19681 tccaagccta aaaatggttc gtcgatgata tataaagaag gctcgatcaa aaaggcgcat
    19741 aagatcatca ctttctgttt cattcccttt gaaaaattag ctgggaacca gtcaagacga
    19801 ttagataacc gaaaagtctc caacaacttt tctgctcttt tcattgctat atccataggt
    19861 atatcgtagg ccatcgcagt gacttcgata tgttctctca atgtcaattc ttcatataaa
    19921 gaaggagtct ctggaatata cccaatttgt ttgcgatatt cttccgtatt tttctttaag
    19981 gatagatgat tgatttctat cgttcctttt gaagcttcca gcagaccgat aatgtgcttg
    20041 atggtcgtac ttttccctgc tccgttcagt ccaatcaacc ctactatctc tccaggctgt
    20101 acatcgaaac tgatatcttt taacacaggg agatgtccgt acccccctgt gacatgatcg
    20161 ataattaaac tcattctttt tcctccgcca tttattttta tttattatac cacgaagaaa
    20221 atgacggtat tttgtttttt tccttacaat agctttaact tatggtaaag ttaagctgaa
    20281 gtatttattc tttatagaaa ggatgtttta tatggaaaac tgtatttttt gcaaaatcat
    20341 caatcaagaa atcccaagct ataaaatata tgaagacgac aaggtctatg cttttttaga
    20401 catctctcaa gcaacgaaag gacacacatt agttgtgcct aaacgtcatg tagcagatat
    20461 ttttgaatat gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta agattgctca
    20521 agcattggaa aaagcttttc cagaaatgaa aggtcttaat atcttgaata acaaccgtga
    20581 actagcctat caatctgttt ttcattcaca tatccattta gtccctcgct acagcaaaga
    20641 ggatgatttt tctattcact ttgtcaatca tcaagatagt tatggatcgg aagaactaaa
    20701 agctattcaa gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac tttttcaaag
    20761 gactgttatt tggagctgct gctggcactg tcggaggtct gttattggcg cccaggagcg
    20821 ggaatgaaac aagaaagaaa ctgatcgaag aattggaaga agcaacaact ctgacaaatg
    20881 atttaaataa cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa gaaacagctg
    20941 aatctgtgat tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat tttaaattcc
    21001 aagccgaacc acgtatcaca caaatcaatg aacaagtaga aaaactaaaa gctgatctgc
    21061 cagagtatcc tgaacaaata taataatgat tattttctca acagatttaa ctatataagt
    21121 gagaaaagtt tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt ttgaaactac
    21181 cacgacaacg tttttaaagg aaatgggata tggtatagtt aatatataaa aataataact
    21241 aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag ctactagtgc
    21301 gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata tcgcaacgat
    21361 gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg aatcatcgaa
    21421 ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat atggcgataa
    21481 agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat taggtgatac
    21541 ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag aatatattcg
    21601 taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag atgacgattt
    21661 gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca aattatcaag
    21721 tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt tctctaaatt
    21781 agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag ttaaattcga
    21841 ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat tgaaagacgg
    21901 tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag aatactacgt
    21961 tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca aagatcaact
    22021 aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta aagtaatcgg
    22081 tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa atgtgttgag
    22141 tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag cttcaacaaa
    22201 gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag aaacaactga
    22261 ttcatcaaaa taaaagaaaa caaaaaaagc gaagacttat gtctcgcttt ttttgttttg
    22321 aatgaaaaaa ataaattttt gctataaatc ttttggtaca tagaaacttc ggttgtccat
    22381 accgaaaatc cgatcggtgt attttcctgg ttctgtttga cgaatcgaag tcaacatcat
    22441 ctgcatcgaa gcatcgatat tgtcgatttg atgtaatatt tctgcttcca ttattcttgg
    22501 acgtacaggt gagccatatt ctaataagcc atggtgtgct aaaaccatat gacgtaatac
    22561 gagaacatct tcgtcagctt cgctgatttt caacgcaaga caggctttag ttatttcttc
    22621 gtcaatcaat accagatgac cgatcaaatt tcctgcaagc gtatattctg tcgacatagg
    22681 accggaaagt tcaatcacct ttcctaaatc atgcaaaatg ataccagcat acaacaatgc
    22741 tcgattcagc tcaggatatt catcggcgat tgatttccct aagtgaagca ttgtcaaagt
    22801 atgataagct aatccactgg caaatgcatg atggtttctt tttgctgccg gaaactcaaa
    22861 aaattgtttt tgatatttat taagtagata gcgaacaata cgattccaat gcgcattggt
    22921 gatctcaaac aacaattgat tgatttcttc ttccatttct tctttcttca aaggggctct
    22981 ttccatgtat aaactaggct ctcctggctc atctaccctt gttaagcgca tatgaagaat
    23041 ctttacttga gggtttcctt gataaacctc tcttttaccg tttagaaaaa cgactgtgcc
    23101 ggctgtaaat ttattgattt cctcctccga agcatcccag tatttaccat cgatagtacc
    23161 tgaagtatct tggaaagtaa aagcaataaa tttttttccg tttttagcga ttcttacatc
    23221 tgcacttttg atcaggacaa attgttcaaa ctgctcatcg attactaaat cacgaatttt
    23281 tttcattatt tgctccttct aattgtttta cgatttggtt catctcatgg taatagctta
    23341 ccatttcttt atcagaagaa aaacaaatta tttgatactt ttctccaaat ttagctaaaa
    23401 gttcagctaa ttgatacttt cgctgatgat cataatgaag ccagccatca tcaatgatga
    23461 tcgggcatat cgaacggtta gcttctaatg agaggtaagc aaatctcatc gccataatca
    23521 gctgatcttt cgtacctgta gataactcat agatgggaaa ttctgtatat ccgtctgtta
    23581 aagtcagtgt atcatttttc agtaatatct tctgataatt tccttttgtt aaaataccta
    23641 aataatcacc tgctgctctt aacaactggg gtaattgtct ttcactcatc tctgttgaaa
    23701 gatcacctaa gaatgtcccg gcgatttcaa gtgctcccca ttcctccagt aattcctgta
    23761 attttgcttt ttgccggcta gcttgttgat aaagttcgtc caatgtgccg tctctttgca
    23821 actcttctac ttgaagttgg agtcgttgcc gattttcact tgcttctttt tcttcttgct
    23881 ggagttggga ttgcttttct ttcagttggc gcagtcttac agataatttc tcttttgtca
    23941 gctcttcagg aaatagtgga gtaagcattt ccgataattc attgtatctt ttcatctgct
    24001 gttcggcttg tttggctttt tgtaaaaaga caggtacttc cgaaggataa gataagcctg
    24061 cttgagtcaa ttgttcgctg tattcttgta tccatacttt ttgtttcttt ttcaattgat
    24121 tgatttcttg cttcaataaa gtattttgct gatagcttct ggcaaatttc atctgttcca
    24181 tctcttgtat aaaacgggaa agcaatacca ttttttctga aattgtctta tcttgtatcg
    24241 gtaaccattc tttaacaaac tcaaattttt ggtcgatttg tcgtagtttt tcttgtattt
    24301 cctgttctct tttatgtttc cgctcaaata cgccatataa tttctgataa tctatgactt
    24361 cctgtccata ttctttcatg agtttgagcg tatccatctt tcctaagtgc cgatgatccg
    24421 caaaacgata tacttcttgc tgaagaatct gtttttcttc tccagttctc ttttgttttt
    24481 cttctgcttc agctaattga gctaaggcta gatcaagttg tcctagtttt tcttgccaca
    24541 ttccttttat ctcagagaaa tcatttttag ttttaatttt tggttgcctt ttgtagaaga
    24601 aatagccaat catgcctgca ccaattagta aaaggatcac tttcaagggc attggtacaa
    24661 atatacctgc aatgattatt gctccgccag ccatcagcat atacggttta gatgaagaga
    24721 ctggtgctat ctctgactga ttttgtaata atgctggata ggtctcttcc aagtcattca
    24781 cttctgcttc aatcatctct tgctgttctt tagctatttg gagacgcaca gaaatctggc
    24841 tttctagatc agcaatcctt ctttcttcag ccagaagatg cttccattct tcttcatata
    24901 aaaattcttc cggtaacgga tttttctgcc aatgccacct ttgttccaaa atcttcaatt
    24961 ctgaatttgt ttctctagtc tcaatttgta atctttgcca ttcttcgttt aattgttccg
    25021 ctgtaaattg aagttgctgg agatctttta gttcctgctc ctgctctaaa taaaaataat
    25081 atctggctga ctgctgatcc atcccgctat gcttttccag tgttgcttct aattcttcca
    25141 aacgctcatt tgtttgctga taccgtttgg agaaagactc cagatctaaa agaagttgct
    25201 catcaggaag acctgtagtg ctgttttttt tgagagattg ccattcttca tataaagaaa
    25261 aattaagttc ttgctgtctg gctaatgaaa attgtttgtt cgcctctttt aattgtgcca
    25321 ataactgctg ctgttgcgac tcgtattcct gttcttgttg gatcaacttg ccaaaataag
    25381 cttcctgttc ttgtttttga tgaatttttt cttgcagctc tttccattca tttaattttt
    25441 gattgattag taatttttgg ccttttttct tgaataattg ttgtgctttc tgataatatt
    25501 cttgccgctt ttggaatact tgagaactgc ccgtgatgcc taaagaaatc agcgcatcat
    25561 gtagctcttt ttctctcaaa tgatctagtt ccatcaactg ctcttgctgg aaagtaaata
    25621 cctgctggaa taactctttc gtcaaaggtg cgatcagttt ttctagtaac tcatcacttc
    25681 ccacttgatc ccccagccac accttcgatt ttccgcgatt ttgctgcttg tagcgctcga
    25741 cagcaaattc accatataca ggatgtttaa gccatatttt gccgccatag gctgagccgt
    25801 cttttggtgt ataatccttt tttttacggc cttttgaagg gaaaccaaat aatattgctt
    25861 gaataaattg ataaatcgtt gattttcctg cttcgtttcc cccatagatc agctgattgc
    25921 ctgaataaaa gtcaaaactt ttttgacgcc attttccaaa agcagcaatc tcgatcctta
    25981 aaatcttcat tcctgatcct cctcaaataa aaaatcttga ttaagcagct gcttggcttt
    26041 ttcaagtgtt tcttcttgat attccggcag ttcgctcaat attctagccg cttcttgatg
    26101 actatatact tcatttaaaa tttgttggaa atcttgaggt gtctgatagt attgaaataa
    26161 ctgttccatc aatttagcag aagcagcaag tggggtcttc gtataaaaag tattctctat
    26221 gagagaaata cgccaaataa agaaatcatt tatcctatct gagaattcct ctaacaaata
    26281 gtctaatagc tctcctgatt gtatccgttc caaaacatct gtattgagat ggtcataatc
    26341 ttttaatttt atctcaatta aactaaatcc atcctctact tttgaaagct gctgacgaat
    26401 gtgaacaatg acctcttttg tcgttcttgc tttcctcaat gatatttcct ttgtcttcca
    26461 atagacctct gctactttaa tcggatgtat ttggcaactt cctttactta attcaactag
    26521 tagaatagat gtagaagctt gttccttttt agtatgacct tgaggcgcac ctggataaac
    26581 aattgtttgc ttttcattta agaccgttgg cacatggata tgccctaaag cccaataatc
    26641 gtatcccttt tcttgcatct ttgagggttg gaaaggagca tagtttcctt tttgagagac
    26701 gccaggttcc ccgtggtaca acccaatatg gtaatctgtg agttctctag aaggaaattc
    26761 catcactttg tctttttgaa tccacggttg acgataacta aatccactga gagagacagt
    26821 ttctccactt tttatcgtta ttttttttgt ctctaccgtt tcggatgtaa ataaatggac
    26881 atttttagga aaggagaacc aataccgctc tttttgatag aaatcatgat ttccgaatat
    26941 tataaataca ggtatatggt tcttttctaa ccgtttcatc tgttctgaaa aatgtttctg
    27001 tatttttaat gaaggacgat tttgatgaaa attatctcct gcaagtaaaa caaaatcaac
    27061 gttatttatt attgcctgtt caatgatatt tgataaaacg gtcagattcg tcttcaatag
    27121 tttttcctgt acagttttat caagagttgt cagtccttca aatgaccgat ccatatgtaa
    27181 atctgccgca tggataaatt tcaccattaa aaatttcctc ctttttttct tttatcatac
    27241 cattttcaat gttttttttc atcaataacc gaaaatttgt tcgtattatg caaagaaatt
    27301 acgataaatg gccgacttca aaaaataagt ttattcgaaa aaaagattgt gacatgattt
    27361 ttgtcacaat cctaaatatc tatgttatat tcgcttcgtt tgacactgaa gaagtactat
    27421 ttgtactgga atttatgctc catttttccg tatttagaat tctatgttct atactacctt
    27481 aattagttta tatggtaaga attctttcaa atgcttgaga tactccgtct ttatcatttg
    27541 aaacagtgac ataatcagct tttttcatga tttcttgatg actatttccc attgcaaaac
    27601 taatatctgc actttcaaac atagataagt catttaaact atctccgatc gcgattctca
    27661 aaggtttctt taaattgtag attgattcca aacgatcaaa caccacacct ttagatacat
    27721 tttgaggtac gatttcaata tttacaggtg ccgattcagt aacagacaca tttagcatct
    27781 ttttcaaatg gttaaatata ttagctttca attgacgatt aggaataaaa ataaaaaatt
    27841 tgtaaatgct gatagcatcc ttttctataa tttcctcaat attattataa gggattgaac
    27901 aaatagattt ttcatactca agctcatagt ttagctcttt gatagaaaat tcactcccta
    27961 cctcgtgaaa aatttttttc attttatttt tataattctg agattcataa cttccgagat
    28021 ttgtatatat tttg
//
LOCUS       DAGCWU010000019        27706 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.019, whole genome shotgun sequence.
ACCESSION   DAGCWU010000019 DAGCWU010000000
VERSION     DAGCWU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.019"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(76..2157)
                     /gene="cadA"
                     /locus_tag="MBZ71_000684"
     CDS             complement(76..2157)
                     /gene="cadA"
                     /locus_tag="MBZ71_000684"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBT4428659.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(2681..3040)
                     /locus_tag="MBZ71_000685"
     CDS             complement(2681..3040)
                     /locus_tag="MBZ71_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBT4428660.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3070..3411)
                     /locus_tag="MBZ71_000686"
     CDS             complement(3070..3411)
                     /locus_tag="MBZ71_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428661.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(3408..4082)
                     /locus_tag="MBZ71_000687"
     CDS             complement(3408..4082)
                     /locus_tag="MBZ71_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBT4428662.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5135..6433)
                     /gene="asnS"
                     /locus_tag="MBZ71_000688"
     CDS             complement(5135..6433)
                     /gene="asnS"
                     /locus_tag="MBZ71_000688"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBT4428663.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6473..7663)
                     /locus_tag="MBZ71_000689"
     CDS             complement(6473..7663)
                     /locus_tag="MBZ71_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4428664.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIETIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7684..8211)
                     /locus_tag="MBZ71_000690"
     CDS             complement(7684..8211)
                     /locus_tag="MBZ71_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBT4428665.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8258..11047)
                     /locus_tag="MBZ71_000691"
     CDS             complement(8258..11047)
                     /locus_tag="MBZ71_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBT4428666.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11194..11394)
                     /locus_tag="MBZ71_000692"
     CDS             complement(11194..11394)
                     /locus_tag="MBZ71_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4428667.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11846..12166)
                     /locus_tag="MBZ71_000693"
                     /pseudo
     CDS             complement(11846..12166)
                     /locus_tag="MBZ71_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12669..13787)
                     /locus_tag="MBZ71_000694"
     CDS             complement(12669..13787)
                     /locus_tag="MBZ71_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428668.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYYEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAIFRY"
     gene            14259..15566
                     /locus_tag="MBZ71_000695"
     CDS             14259..15566
                     /locus_tag="MBZ71_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBT4428669.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSESKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            15685..16883
                     /locus_tag="MBZ71_000696"
                     /pseudo
     CDS             15685..16883
                     /locus_tag="MBZ71_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            complement(16910..18178)
                     /locus_tag="MBZ71_000697"
     CDS             complement(16910..18178)
                     /locus_tag="MBZ71_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBT4428670.1"
                     /translation="MEKQLPSTVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(18341..18988)
                     /locus_tag="MBZ71_000698"
     CDS             complement(18341..18988)
                     /locus_tag="MBZ71_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBT4428671.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(19396..19899)
                     /locus_tag="MBZ71_000699"
     CDS             complement(19396..19899)
                     /locus_tag="MBZ71_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4428672.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(20022..20786)
                     /locus_tag="MBZ71_000700"
     CDS             complement(20022..20786)
                     /locus_tag="MBZ71_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBT4428673.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DVEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSK
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            20894..21169
                     /locus_tag="MBZ71_000701"
     CDS             20894..21169
                     /locus_tag="MBZ71_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBT4428674.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            21765..22715
                     /gene="yidC"
                     /locus_tag="MBZ71_000702"
     CDS             21765..22715
                     /gene="yidC"
                     /locus_tag="MBZ71_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBT4428675.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(22894..23868)
                     /gene="lacD"
                     /locus_tag="MBZ71_000703"
     CDS             complement(22894..23868)
                     /gene="lacD"
                     /locus_tag="MBZ71_000703"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBT4428676.1"
                     /translation="MKKMSIEKQECLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDESVEINVQKQIVIERIDSECEAEGMPFF
                     LEILSYDATISDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVKGYG
                     QETCYSQAEASTYFYEQSKVTEQPFIFFSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(23865..24323)
                     /locus_tag="MBZ71_000704"
     CDS             complement(23865..24323)
                     /locus_tag="MBZ71_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4428677.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKTEIATILTKEIGEDI"
     gene            complement(24320..25783)
                     /locus_tag="MBZ71_000705"
     CDS             complement(24320..25783)
                     /locus_tag="MBZ71_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4428678.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEGLEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(25765..26715)
                     /gene="pfkB"
                     /locus_tag="MBZ71_000706"
     CDS             complement(25765..26715)
                     /gene="pfkB"
                     /locus_tag="MBZ71_000706"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBT4428679.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMGCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            26858..27607
                     /locus_tag="MBZ71_000707"
     CDS             26858..27607
                     /locus_tag="MBZ71_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4428680.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFTDRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMREN"
BASE COUNT         8030 a         5928 c         4849 g         8899 t
ORIGIN      
        1 tatctatttt ctttacattt ttcattaaga aaaggatata aaaaaaagag gacgttcatt
       61 cgtcctcttc attgatcaac gtttattttt tacatgcaag cttcgcatgg cattcaaaac
      121 agctaatact gtcaccccaa catctgcaaa tactgcatct cccattgatg caaaaccaaa
      181 agcacctaag gcaagtacca aaatttttat cccaatagca aagacaatgt tctgtttgac
      241 gattttcaat gttttctttg ctaaatggat tgcttctgca atcttcgatg gttgatcatt
      301 catgatgact acatctgctg cttcaattgc agcatcgctt cccaatccac ccattgcgat
      361 tcctacatct gctcttgcta gcaccggtgc atcgttgatt ccatctccga caaatgcggt
      421 ctttttattg tttctttgta agatttcctc taaccgctgc actttatctt ctggaagcaa
      481 ttcgctatat atcttgtcca cgcctacttg cttcccgata tgatcagcaa tttttttcga
      541 atcgcccgtc aacatgactg tgttcttcac accagccttt tttaattgaa ccaatgcgtc
      601 tacggcatct tctttcaagg tatctgcaat aacaagataa ccactgtaac tttgatcgat
      661 cgctagataa agcaatgtac cgatttcttg acttgaagga aaagagattt caaatctttc
      721 catcaatttg tgattacctg ctaatacttg tttgccatca acttcagcta agatcccttc
      781 acctgcaaat tcttggatat tagctgttgg taaaagcgga cctgcgtagc cttctaaaac
      841 agattgagca atcggatgtg tagaaaactg ttctgcactc gcaacatatt gcatgaatat
      901 ctttggatcg atttttgtat cgatcgtctg gatcgaaaag tcacctttcg ttaatgtgcc
      961 ggttttatcg aataccactg tctcagtttg tgcaagcaac tccaagtaat ttgaaccttt
     1021 tactaataca ccgattttac ttgctcctcc gataccgcca aagaaactaa ggggtacgga
     1081 gatcactaat gcacatggac atgagatcac taaaaatgtc aatgctcgat agatccattc
     1141 agaaaacgcc gctccagaaa caacaagtgg aggtaaaaca gctaataaaa cggccaatcc
     1201 tacaacgact ggtgtgtaat agcgcgcgaa tttagtaatg aaattttcag ctggtgcttt
     1261 tttactgctg gcattttcta ctaattctaa taatttgctg atagtagaat tttcataaga
     1321 tttttctact tgaactaaaa atggtttatt tttattgata aatccactta atactgtttc
     1381 accaacatat acagatttag ggactgattc tcctgtaagt gctgacgtat cgactaatga
     1441 ttctccttcg atgatcgttc catctaacgg tacacgttca ccaggcttca caagtacaaa
     1501 ctgaccgatc tgcacgtctt cagggttcac ggtcatcaat ccttccgtcg tctgcacgtg
     1561 agctgtttct ggacggatcg ccatcaattc gcgaatcgat tttcttgaat gactgactgc
     1621 caatccttgg aaatactcgc cgatttgata aaatagcatc actgctacgg cttctggata
     1681 ttcaccgatc aacatagcgc caatcgtagc aacagacata agaaaattct catcaaagat
     1741 ttttcctttg acgatattca tcactgcctt cttcactaca tcatatccta tcagcagata
     1801 aatcaataaa taccctatca agcgaattgg ttcattaaaa tcaaataata gcaataaagc
     1861 gatacctgca agggaaaata aaatccggaa cagcgtattt ctctcttttt catttccatg
     1921 ttcagatact ttttccttat ctatttctgt tactttgaca tctggttcta attccttcac
     1981 tacttttttt atttcttgtt caatcgtagc caaatgatct tcctctgcgt caatcgttag
     2041 ttttccagaa gtgaagttta ccgttgcttc cttgactcca ttgatcttct gaactccttt
     2101 ttcgattttc attgcacagt tcgcacaatc taaaccttct aatctgtaac ttttcatttt
     2161 aatgacctcc tcgcctcaac atatgaatat ctcttcataa ctaattataa atgaacggtt
     2221 attcatatgt caaggtatcc gaaagaaaaa atgaaaaaca tctgcatcgg aaaatttctt
     2281 ccatacagat gttttggctt attctttctt tttcttttcg atcattgcca ctgctcttat
     2341 ccaacccgca cttgccttct tgatcttgac aggatagcaa ctaagtttga agcctgttgc
     2401 tggcaatttg tctaaattca ttaatttttc gataggacaa tatctttttt caatccctgc
     2461 aaaatgtcct tcccagatat tcgaagctcc actacgcttt caaaaatata gaaagagttc
     2521 tttaatgaaa attatttcaa ctttccataa acagtaaccc catataaata aaagacgtaa
     2581 aagtcacatg gaagaactta tacaggagaa cgcatagagt caggtgaaat acatgtatcc
     2641 cacctgactc tatgctttat ctaaccttta ttattctctt ttatacaatt tctgcaccga
     2701 atggcatcaa cgccaatcga gcaagtttga aagattgctt ggcaaaaggt atccctatga
     2761 tcgtgataca tagaaggatg ccgctgacga catgtgcgag agcaattggt aggcctgaaa
     2821 cgatcagcca gatgatatta acaatcaatg acatgccaga ctggtgataa attacttctt
     2881 tgccaaaagg cgcaaaagaa agggaggcga tcttaaaaca ttgcataccg attgggatac
     2941 ctatgatcgt gatacaccaa agtatccccg ctgctaacca gctaagacca ccgattagtc
     3001 cgccgaatat aaaccagatg acatttccta aacaactcat tgtatttctc cttctattac
     3061 tgtttttgat taaaactggg caaaaaatga ttttattctt ctttttttag cttccagtat
     3121 caaatattct gcaaattttt tttggatttc ttccgtattt tcttttgtta ctaatgtgtg
     3181 ttggattagt tttcctgatg gtcgctgatc caatcgttct tctgactgtt gcacgtccgc
     3241 ttgcgcaaat ccgataatcc gtttttcttt tgttacagca acgagcaata gtttattctt
     3301 atcatttagg tcagctgccg tttttgcttt tacgaatgat tctggatgat cagtaatcag
     3361 ctgattgatt tgaggtagat cttcttttac tgcccaccgt atttccatca tttctcactt
     3421 ttctttcgct catttttatc tccactaaaa aaatcgactg cggcaattgc atctatccag
     3481 ctttgtgcaa tgatctcatg tccgccaatc gttgggtgga ccccatcttc tcttgtatat
     3541 ttcgaaaaac cataaacaat ccctaaagca ttgaatatac catctaaagg aaccagcaat
     3601 gcgccatatt ccttagcaag tttacgaaca atttggattt tagggtctaa gtctttacgc
     3661 cagccatgac ggtcgtcggt tataggaagt acaaacggct ccatcaagat gatgttgcag
     3721 attcccactg tttgaagttc atttagtaat tttcgataaa cggattcaaa ttgctgagca
     3781 gatttctcag ttccgaatat agcctgatcg ctagtattat gccaggtatc attgattccg
     3841 atcaaaatcg atacatagtc aggttgtaaa gctaaacaat cttcttgcca gcgtacttgc
     3901 agatctgcaa ttttgtctcc tcctatccca cggttcatgc attcaaattc catcgttgga
     3961 tattgggaga ttagttttcc ggcaatcaaa agtggataac ctttacctag atcagttgct
     4021 tctgctcggt ctcttcctgc gtctgtaatg ctatcaccga taaataatat acgttttttc
     4081 atttgtcaaa aactcctttt tgaataaaac gatgattttc ttccttatat tatcccataa
     4141 gccctatcaa gacgaaagag aaaatatatg tataaaaaat gacaccagcc tagtcccttc
     4201 aaacaattga atgaacgctc atcaagtgtc tagtttttta tgctattttt ttcttaacgt
     4261 gccggattcc ccttgattcc ttcgttgttt ggtccagaga gatcgatcac acctaagctt
     4321 ccgtcaatca gaccactgat gacgccatca gaacatatat ttataggctc atctaagact
     4381 tctagtaaat gtccctttac taatatctct tctagattgc ttgggataga ttggtcataa
     4441 ctttatctgg tccgatgatt tcaagggtga attgaactta catatggatt attgcatgct
     4501 gaaaatacgg acctactcca tcttcgaatg ttactattag tttgttttca ggtgctagat
     4561 gctgctttat ttcaacagca gaagcatcat caaatcagat ttccatttga attaccttct
     4621 ttgtaaatgt ttgattgatt gcgatttcgt tatagaacct tgtgaatcaa atagcaattt
     4681 cttttgcttt cttctcttgt ctgctggaaa cttcctttat cccgtatttt aaaaaaagaa
     4741 ctgcgagagt tcgcagttct tttttagaat aaacggtgct ctccggccaa ctcctcggtc
     4801 acaagtcaca atagtcatca aacatgaaaa actgtttttt gaaattactc cttgtgactc
     4861 ggagctgaac ggcaggcgca caacctcttc cttaaacggt gttcaaaagc cagtttctcg
     4921 gtattaagcc aaatttatgc aaaaatgtgg aaagccattt ccacaaaatt tttcttaata
     4981 ctcgaaacta aacagctttt tcacaacctc tccactaaac ggtattcaaa agcttacatc
     5041 tgcggtaata agtcccgcca attcaaaaac atgaacagct gtttttgctt ggctgttcta
     5101 attacttgat gcagaacagc tttttcatca cctcttatgg ataaattcgg ttcaacaaac
     5161 gtgggaatgg aatagcttca cgtacatggt cgataccagc aatccaagtg actgttcgtt
     5221 caagacctag accaaaacca gagtgcggaa cagaaccgta tttacgtaga tcaagatacc
     5281 atgaatattc tttttcatcc aaaccgtgat tacggatttg ttctagcaag aaatcatagt
     5341 ctgttgcccg ttcgcttccg ccaataattt caccgtaacc ttctggtgcg atcatatctg
     5401 cacagatcac cacgtcttct cttgttggat gtggtttcat gtagaatggc ttgatggctt
     5461 ttggatagtt taggataaag acaggctgtt cgaatgaatt cgcaatgaac gtttcatgag
     5521 gtgaaccaaa gtcttcgccc cattcgatgt catcaaatcc atttttcttc agtaattcga
     5581 tcgcatcatc ataagtaata cgagggaatg gcaattttgt ataagttttt aagacttctt
     5641 tgtctcgtcc tagtacatct aatgcatatt cgcaatgatc taaaacattt tgtactaaga
     5701 atgctacata ttgttcctgt acttctaaac tttcttcttg atgagtgaaa gccatttctg
     5761 gttcgatcat ccagaattcg atcaaatgac gtcgtgtttt tgatttttca gcacggaaag
     5821 tcggaccgaa tgaaaagact tttccgaaag ccatagcagc agcttccatg tataattggc
     5881 cactttgaga taaataagct ttttgatcaa agtaatttgt ttcgaataga tccgtagtgc
     5941 cttctggtgc agaaccagtc aagatcggcg gatcaatttt gacaaaatga tgttgattaa
     6001 agaattcata cgttgcacga atgatttcat ttctgatctg cataatcgca tgttgtcttg
     6061 aagaacgcag ccataaatga cgatgatcca tcaagaagtc tgttccgtgt tcttttggtg
     6121 tgatcggata atcgtggctc tcgcttacca cttccacatt tgtaattccg atttcataac
     6181 caaattttga acgactgtct tcacggattt ctccagttac taacacggat gtttcttggt
     6241 gtagattttt agctgtttgg aaaacttctt ccgacacttc gctttttaca actacacctt
     6301 ggaaaaaggc tgttccgtct cttaattgta aaaatgcgat ttttccgctt gatcgcttat
     6361 ttgcgaccca agcgccgatt gttactgttt ctcccacatg gtttttcgaa tcgatgattt
     6421 gaatttgttc cacgtatgac tctcctttta tattccttga atttctttag gattaatttt
     6481 gacttttact ctcaataaat tgcttgattc tgcgtaccgc ttcttctaac gtagcgcgat
     6541 cagtagcata actcatgcgg acattttctg gtgccccaaa gccttcgcca gtcacaagcg
     6601 ctacccctgt ttcttctaaa agatcttcta cccattgtgt gacattttca tatttacaca
     6661 tgcgtagcgt ttcttttaca ttaggaaaaa gataaaaagc tccttgtggc ttttcaagag
     6721 aaacacccgg cagttctgcc acaagcgaat ataacgtatt caggcgttct tcaaaggcgt
     6781 tccgcatttc ttccactgta tcttgctcgc ctttcaatgc ttcaacagct gcatattgac
     6841 tgactgctgt cggattactg gttgactggg aagcaacagc gatcattcca ttgatgatca
     6901 caggatttcc caccgcataa ccaatgcgcc agcctgtcat cgcatatgtt ttagacacgc
     6961 cgttgatgat gatagtctga tttttgattg cttctgaaat cgttgcaatt ggagtaaact
     7021 cattgccatt ataaactaaa cgaccataaa tatcatcagc aactatcaaa atatcatttt
     7081 ttactgccca ttcaccgatt gcctgcagtt cattcttgct ataaatcatt ccagtcggat
     7141 tcgatggaga attgatgatt acggcttttg ttttttctga acgtgcttgt tctagctgtt
     7201 caacagtaac tttccagcta ttggattctt cccctttcac aaagacagga agtccttctg
     7261 ccagttttac ttgctcaccg taactgaccc agtaaggcac aggaatgatg acttcatctt
     7321 gcgggtcaag gatcgtttgg aaaagggtat acaaagcaaa tttcgctccg tctgttacga
     7381 ctgtttgtga aggttcatag acaagtccat agtttttctt cagataatca acgattgcct
     7441 gtcgaagctc tgggattcct gctgttggtg tataatagct agctttccca ttgcggattg
     7501 tttcgattgc tgcttcttgg atgttttctg gtgtcgcaaa atccggttct cccacagtca
     7561 aactcagcac atctctgcct tttgctttta atgccttggc ctttgccgca gctgccaaag
     7621 tgacagacgg ttctaatcgt tttgttcttt ttgataactc catcccattc attcctttac
     7681 cttttaaatt cccttgattg tcttgacttc atctccagat tcaaagccaa tcaggtaata
     7741 attcagtttc ccatttgttt cttttcctac tacttcccac acaggctgat catcatacat
     7801 ccctaaagca gctttttcga tttggatttc aggatgggcc gcagcaattg tctgttttgc
     7861 ttcgttttca gtcagtccct ttgtttgatc caatactttt actttttcac cagatttagg
     7921 aatgataacg gcgatttctt ttcctttatc atttgtacct gtcagtgaaa agtatgtctt
     7981 ctttcgagta aaccaataaa aatgatcggt ttcctcaaga tttgcatatt tctttgcgat
     8041 ttctactgct tctttcttcg cctgcaccgc cggacgactg gaacgcaagt aaaaaatcga
     8101 gctgatcacg atgaccgcta ataaaaatac gatcaagcca agtaacacgc gattaatcgt
     8161 tttttccttt gtctgttttt tcttcaaggg tcgtcgctcc tttatcttca ttccttttca
     8221 ttatagcaga ttttaattat gaaaaaagac agctcctcta acttccttct tcatttttct
     8281 ttaagaattc atggatatcc tgatagattt cctccatagc tagctctttg atcggtactt
     8341 ttttcggtaa tgcatggatg atccgtgcgc catattttgc agtgactagt cttcgatcca
     8401 aaataagcaa tacgccacga tctgtctccg tccttatcaa gcgacctaat gcttggcgta
     8461 acttcaaagc tgctttaggt acggattcct ggtaaaaagg attgatcccc tctgattcta
     8521 aataagcgtt cctagctttg accatcgggc gaagcggatt atcgaagggt aaacgtgtga
     8581 ctacaacgat ctgcaaagca tctccaggca aatctacacc ttcccaaaag ctgtcagcgc
     8641 caaacaatac gcttttttct gacaaaatga agcgtttcaa aagcttctcc cggcttccac
     8701 caaacccttg agctagtatt tctcgtcctt tttctagcat agacagatgc attttctgat
     8761 agaccttctg caaaacatca tgagaagtga ataacacaag tattggccga tcttcttgtt
     8821 cgatcagacc tgtaagtgct gtctctaaat aatcgatata tgcttgattg ctgacttgat
     8881 ggacggcagg tgcctctttt ggcacataga tccttgcctg tttctcataa tcataaggag
     8941 aggcaatcac tttaagaggt gctgcttcaa tgccaagcct cttagagaga tagtgtcgat
     9001 tagggccgat cttcagcgtt cctccaatat aaagaatacg atcatatcga ggataccaaa
     9061 cagtcgacgg caacatagcc gcttgaaaat catggagatg aaaaatcatc tgctgcgctg
     9121 tgttcccagg aacgaaccaa tggatcaaat gatctcgcca gtcttccagc cattgatcca
     9181 tcagcaagta ttgcttgtct aattgatcaa ataggctata taaacttgct aaatcgatcc
     9241 gcatctggtt tgtccagctt tcttggataa tctcaaaagt cagacgcatt tgtgactgta
     9301 gctgctggat ctcctgataa aaaagctgca ggtgatcaat ggctgtccca gattccagtg
     9361 gcagctgatc caacatctct tttgtcatca tgacttcttc tggatactgt ccgttaggtt
     9421 gcttgccgct cactaaactc attaatcctt ctgccataca aatctgtgct tcgcatagcg
     9481 cagaaagttc ttctcgataa atcgataacc agcgtaaggc ttcatgttct ttcggtaaat
     9541 agctttgtat tcggtcaaac agctgatttg gttcttctaa ttgctggatt tgtttatgga
     9601 aatagttaat acttaatcgt ttgtcattta ctttttcagc gatttccggc aaatgatgta
     9661 cttcatcgat caacaaaaat ggactagctg gaagcaatgg ttcttttcgc tgtgtttctt
     9721 gtactaaaaa cgcatgattg acaatcaaaa aattactttg cctgattttc ttttgcaaga
     9781 aacgaacaaa gtcttgttcg taaaatgact gatttcttgc taaaaatcca atacctctgt
     9841 gcgcgatatc tgcaaagaat gaatggttca aatttgttaa atgtaattca tccaaatcac
     9901 cagtcttcgt ttgcgtcagc caaactaaga ccgccatttg atacaaagca tattgttttt
     9961 gttctggcgg ttccacaagt gtgcctttaa aacgctgcaa atcaatatag tggcgatagc
    10021 ttttgataat tacaggttgt atgggctgat ctagcaattg attcagcaat ggaatgtctt
    10081 tttctatgat ctgctgctgc aaaactagcg aaacggtact gatgacagcg ggtttttcag
    10141 gtgtagccaa ataactcaat ggcagcagat aaccgattgt tttacccatt cctgtagctg
    10201 cttcgatcat caaatctttt gattcatctt ttgtgaaatg gtcgtagacc gcattcatca
    10261 gtctgttctg ttcttttcga tacatcagtg ttttaccaaa cattttttct tttgcttgtt
    10321 tttttcttgg atatgtccgc tcgccataat ggacggatgt gaatagttcc acttcttttt
    10381 tctgcaaagc tagcccatcg acgatttcta atgagggatc aagtggctct ggtttttctt
    10441 tcatttcttc aatcacatga taaatgaaac gactcgtatc catacctgtg tgtctactta
    10501 atcgcgcaat ctgctctaac gtgatgattg gcaattttct catccgttct tcgatcagca
    10561 aaagaagttg acctgtcact tcggcatcac tatctgcctg atgaggattc tcatgaataa
    10621 atccaaggct ttctgacaaa tcagctaagc gaaagcttgg ttccgtcggc aagaaaattt
    10681 gtgcaagttc tactgtatcg atgcctggta tcttcaaact aggaagtccg catcgcatga
    10741 gttcatggtt caaaaaatta taatcaaaat aaatattatg cgcgacaaat atggtgtctg
    10801 ccaaaagatt gtaaatcgtt tgggcgacat cttcgaaata aggcgctttt tggacgcgtt
    10861 gattcgtgat ccctgttaaa tgctggattt gttttgaaat cttccgccct ggatgaatat
    10921 cgatcgaaaa acgtgaagtg atccgacctg attcaatcat gacgcagcca aattggatga
    10981 tccgatcttc ttttggattc gtcccagtcg tttcgatatc aacgactgca taagttcttg
    11041 gtttcaaaat atcttcctct tctcctgcct ctaatgttct tttgaaatta tactacaaag
    11101 tgcaaaaaaa tactctcatc gctttttaaa gaaaatagac aaaaacgccc agacacgcaa
    11161 atgtctgggc gtttattttt tagtataggt taattaagct ttagtaacgt ttgttgcttg
    11221 aggtccgcgt tggccttctt caacatcgaa agttactgct tggccttctt ctaaagtttt
    11281 gaatccgtcg ccttggatag ctgagaaatg aacgaataca tcgtttccgt tttctgcagt
    11341 gatgaatcca aatccttttt ctgagttaaa ccattttact gtacctgttt ccataataaa
    11401 aatcctcctt gtgtatcaac acatattttg caattacttg aatggtgtat atctggaaga
    11461 tgtttatttg aaacaagcta ccacagatta ccgaacaaaa actactccta tagtatatca
    11521 taccgtttac aaaaaagcta gagtttttcg acttttttta ttaaaaaaag ttttctcagc
    11581 gattacataa agtgaagaac cgaacttttt ttaaaataaa cgacatattg ttcagttggt
    11641 gtttttttgc tctgatgttc ttctttaaac taaaaaaatt attctttctg actacttatc
    11701 ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg
    11761 tatttctgat agcctatgac agtgaccaga agaacaaggc aaaccaatac gatcaacgag
    11821 attagttttt cttttttgct catatctata cctcataaat aacaggatga tgtccatcat
    11881 atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac
    11941 tttcttaatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt
    12001 cttctgtttc aggaatcgca gaatcaagat ttggtgtctg tacataaaca tttttgtaca
    12061 gacgtgtgcc tgtaatagga gaccgctgtg tttcttcagc aaaaactggc tgacttgtta
    12121 aacttaggga tagaaacagc aaacaccata acagtctttt tttcataaag acttccttat
    12181 cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc
    12241 ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc
    12301 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
    12361 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
    12421 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
    12481 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
    12541 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggcaaa
    12601 atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac
    12661 aacctctttt aataacgaaa aatcgctgtc agactctttt catctggcgc atctttttga
    12721 tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc attcaacact
    12781 tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta
    12841 gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
    12901 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
    12961 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
    13021 gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga
    13081 ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa agaatccttc
    13141 aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
    13201 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
    13261 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct
    13321 tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt catggagtcg
    13381 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
    13441 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
    13501 taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
    13561 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcgtaatat
    13621 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt
    13681 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg
    13741 ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa cgtacccctc
    13801 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
    13861 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
    13921 agttatcttc ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat
    13981 gctgataaac taagaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
    14041 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
    14101 aacacagcct attcctgtaa aagtacgtaa aaacgaccgc taaccttcca taacgtgcgt
    14161 tcttatccta aagttttttc agaaacattg aaactctctc ccctctctga taaaatttta
    14221 atttgtagcg aaacaaatta attacaagga gatcggatat gtccataaaa aaaacacgtc
    14281 tagtcctatt attggctagt ctcattcttt gccttgcttc ttttctacca gctgttgctg
    14341 tgcaagctga agatacgttc aaagtaaatg caaaagcagc ttttgctgta gatgcgcaaa
    14401 gcggtaagat tctttatgat caagatggag aaaaaaccat ggggatcgct tcaatcacaa
    14461 aaatcatcgg tctttacatc gttcttgatc aagtaaaaga aggaaaacta tcatgggatg
    14521 ataaagtttc gatttcagat tatgcagaaa atttgagtat caccccagat ttatcgaatg
    14581 ttcctttgca taaagaaaac acctacactg tcaaagaatt attcgactct gcaatcatcc
    14641 agtcagccaa tgcttcaatg gtggccttgg cagaaaaaat ctctggcagc gaatctaaat
    14701 tcactgaacg aatgaaagaa caattgaaag attggggaat caaagatgca acgatcgtta
    14761 atgcatcagg actgaataac tcttatcttg gcgaaaaccg accagaaggt actggagaaa
    14821 acgacgaaaa tcaaatgtct gctcaagatg tggccatcgt tgcccgccat ttgatcttgg
    14881 attttccaga aatactggac gtatctagca ctacgacaca aatgtttggc gaaaacacgc
    14941 aatcaccagt ggaaatggtc aactggaact ggatgctccc aggattcgtt aattacaagg
    15001 aaggcgtgga cggattaaaa actggtacga ctgagcttgc aggtgcttgt ttcgttggta
    15061 cgatcactaa aaacgatcaa cggatcatca ctgtcgtatt gaatgcggac ggccatgcgg
    15121 aaaacccgag tgcccgcttc aatgaaacag accgtttgat ggactactgc tatgacaatt
    15181 ggtcagaaaa agagctcgga aaagctaacg caagtattcc tagcctaaaa accattgatg
    15241 taaaagacgg aaaagaatca acagttcctg ttgtgcttaa aagccctgta aaagtatggg
    15301 tacgaaacga tatggatact ggcaaattaa cgatcacgcc aacacttgac aagaagcaag
    15361 tcactgacaa cgagatccaa gcacctatgg ataaaggtac aaaaatcggt accgctacga
    15421 tcaccttggc agacgataaa ttaggctatt tagaagacaa caatgcgcca tcaacagaga
    15481 tcattacgga taaaacagtg gaaaaagcga atatgttcgt gattggctgg cgacgcgtcg
    15541 cagaattttt cggcaacttg ttttaattga acaataagaa aagcatcttt ccatcggaaa
    15601 gatgtttttt tgcttttact agtttaaata acagcttttt tcatctattt tcgttatact
    15661 gtataagaaa aaggagttga atttatgaat ctaaccagag atctgatctt tttagttctc
    15721 tatcttattg gggtattctc tatcgtttat tggcgaaaac agaagaagga tccttccttg
    15781 ttcttttacg gcagtacgtg gacatttacg gtaatcttaa cttattttgt cgtgttggaa
    15841 attttttatt cgacctttta ttttttgatt ccattgttgt tctttagtat ctttgctttt
    15901 tcttatttca cagaaaaacg aaggctgctg aatggtcttt tattcaatgt tttcctcatc
    15961 agtttcggca tctacttgtt tgtcttgctg tatgaaacgc aaaatatttt ccttggcgga
    16021 ctgatcgcct tgatcacgat tccattgcta cttgtccttt tatttggtat ttatggattg
    16081 attgtttttc ttttttggaa cggggtcaca gttttacgta gagaatcgca ttcattagct
    16141 aatcttttaa cgttgatact agccattttt cttacactgt ttcttgtttt tgactttttc
    16201 ttgttaaaat atttgccgca atggataaat gctttgtttt tctgcgttcc cctgatcttg
    16261 atctatctat ttatcgtatt ctacaacttt ttgactgtct cattcttgta tcagttcaac
    16321 cggcctcgat acaatcaaga cttcatcgtt gtgttaggcg ctgggttgat caatggagaa
    16381 acagtttctc ctcttctagc caagcgaatc aataaggcaa ttgcttttta tcgggctcaa
    16441 agccgtgcta cattaaatcc gcccatctta ttgatgtctg gcgggcaagg agcagacgaa
    16501 aaagtaccag aggccattgc gatgaaacaa tatgccatgg aacaaggaat tcccgaaaga
    16561 gacattcttg tcgaaaccaa ttcgacaacc actttggaga acatgctcta ttcaaaggaa
    16621 atcatggatc agcaaatgaa agggccatac cgcgcgatct tcagttccaa caattaccac
    16681 atttttagag ccggtcttta tgcgcgacaa gccaaattga aagcaaatgg tattggtgca
    16741 aaaacagctt tctactactt gcctaatgcg ttttgagaga atacatcgcc atcttgatga
    16801 tgaacaaaaa aagacatatg atcatttgcg gattgctttt cgcaggctcc gcattcttat
    16861 cgctgattac tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt
    16921 ttttttcttt cattctctta actattggca aaaagagtat cccttttttg accgctactt
    16981 gctgagcaga ataaataccg ctgttgcctg aacacataaa gctgatcaag cagatcataa
    17041 agaagtatac tgccgcttgt gaaccgaaca attcgatccc catcacaaag caggcaatcg
    17101 gtgtatttgt cgcaccagaa aatactccga taaagcccaa tcctgcaaga aaaggaacag
    17161 aaacatggag caaaggcgct agtgctgcac caagcgttgc accaatttca aacaatggtg
    17221 tcacttctcc cccttgatat cccgcgccta aagacaaaac tgtgaagaat aacttcccaa
    17281 taaaatcata gacatgtgcc tgtccattaa atgattcttg aagtaacgga agactcaatc
    17341 cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac aactacagct ccacctatcg
    17401 tatttcgcca aacaggatga ggaatccatt gtgcataccg tgcttttaaa aagacgatcg
    17461 aacggctgaa tacccagcca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga
    17521 acagcaacat actccattca gggatcacac ccatttggta atgtaggtga gaaacaccat
    17581 agctttctgt cataaaattt gcaaacagtg cagcaaagaa gctagggaag atcgcttctg
    17641 ttcggacttt cccgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc
    17701 caaatacaga actaaaaccc gcactgattc cactgataat caagatttcc cgttctgctt
    17761 tatccaatcg gaacaaacgt gctgtattat ctgctaacgc tccccccatt tggactgctg
    17821 ttccttcacg gccaacagat cccccaaata aatgggtagt tatggttcca aacagtgtaa
    17881 gtggaataag tcgcaacggg attttttctt ctccaccatt tccttgatcg atcactaagt
    17941 tgtttccacg actagaaaga ccgccgtgat acgcatagag ataagcaaaa agcgcaccac
    18001 taactggtag tagataaagg agccaaggat gtgatagtcg gatatctgtc acaactgtca
    18061 ggctatttaa gaaaaaagca gacaagctcc ccatgaatat accgagaatc gctgtgatca
    18121 ccagccactt cagcatgtac actaaaatgc gtactgtact cggtaattgt ttttccataa
    18181 aaaattcctc ctcaataaaa aaacgcctac taaagaaagg ggctgtcccc agactttagt
    18241 aggcgtcatt agctatcttt cgttagcatt taaaggcgaa caccatcacc tattcacttc
    18301 cattaaaaga tagcatattt ttctgtaaaa aacaataaaa ttatcgttct ttctttacac
    18361 gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg ttgattacat tggtcactat
    18421 catgacaaat ataatttccc tttttcgtat acgtgccatc agaacctgct ttcgttgtag
    18481 aaagaaaaag cgaaacattc gatgttttct gacagattgc acagattccc ttatttatcg
    18541 ttggtgataa agtaccgcta atccctttta atttttcatc ttcgtaatag accatgaatt
    18601 ttttctgcgt acctggatca ttccagccga tgtaagtatg ttcttgcaag tcgatttcat
    18661 tccaagctgg ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc
    18721 taggctgcat aaacggctga acgatttgct tcagctcctc caatgctctt tctgcttgcg
    18781 cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt atcgatttct ggcagttcgt
    18841 gatctaatat gtctcgaatc ttttcagctg ctagtgcctg aactgtttgt accgtttttg
    18901 gatcattgac agatgagtac gcatttaata actgttctac ttctcttttt ataaaaaaat
    18961 attggtatgg ttttattgtt tgttccatga ttttaaagtc ctttcttcta accaaagtgc
    19021 atactttgta gaaaaaagtg atcttctgca aagtatgtgt tacctgaaga tcccggactc
    19081 ttttaaataa gatacgtttt gcatcttatc atcaccccct ttaatgctta ttattctatg
    19141 tacatgattc attcgataaa taaaagtaaa ccgtatcttg aacagatagt caactgcaaa
    19201 cagaaaaaag gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat gaatggtgtt
    19261 caaaagcttg catctgcggt aataagccga aacttcacaa aaatttgaga agtaattttc
    19321 gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcaa tatggtctat
    19381 tattggtttt acctgttaaa tcgaggattc tttcccaaag taacgttgcc atctcttacg
    19441 catacttgca gacatgggct gagccgcttc tttgactgcg cagtatttgt ccacgaatgt
    19501 cacaatatat ccttctttat acttaggcgg tgcgatcgtt gcaccccaca tgtgcttcaa
    19561 aatgatatct cgctcaagat ccgacaaatc ggtgattttt tctgcattct ttacagcaat
    19621 tcttggatgg atatacgcat gtgtcccctc gtcaaatttc gtcgtacgcc aatcatagta
    19681 gaacagatca tgcaaaagtc cagcacgcgc tgttgcttta gcattgccac cccatttttt
    19741 tgcgagcaga taactatagt aagaaacact gatggaatgc tccaaacgag tggaatgaac
    19801 atgctgtgtg taatttgcta attttttcac tgcttctgtt tctaaaagat cttgtacata
    19861 agacatatat tcttcgtctt cacgccattt ttctgtcata attatcgatc tgccaccttt
    19921 ttgaatttca ttttcttctg aaattgatct aagtatacca ccagtgttcg gattattcta
    19981 gtcgctctag ataaaatagc taaagcttaa tagttcctta atcataaaga aaaatgataa
    20041 agaacgatcc ctgctgcgac tccgacattc aatgattccg catttccttt cattgggata
    20101 tatacgttct gatcagtcaa agacaagatt tcctgactta ctccctgacc ttcgtttccc
    20161 atgattatag cgaaatcctc tgttttttct actttgtcta atgcatgtgc ttcttcattt
    20221 aattctgtac cgtacaccgt aaaccctttg cctttcatta cagggatcac ttctgccaaa
    20281 tctttttgta acacaggcaa atgataattg cttccctgca tgcttcgcag cacctttgta
    20341 ctataaatat ctgccgttcc tttacctaat aaaacaccag ccataccaaa cgcatctgcc
    20401 gtacggatca tcgttcccac atttccagga tcctgtacat tatctagtaa aagccagcct
    20461 cctgaaaaat ctggcgtatc tttagatgtt aacgtcatca ctgctaagat cccttgcggt
    20521 gtaggtagat ctgacatagc ggtcatgacc tcatctgaaa ctagaaaagt agaaatctct
    20581 ttgtttacag ccagccagtc tccccattct ttcatacctc tctgtgtcac aaaaagttct
    20641 tttatttcta catcagacga cgcagcttct tcgatcagat ggaacccttc caaaagatat
    20701 tctgcctttt cttctctgta tttcttttta tgtagttttt ttaattcttt gattcttgga
    20761 ttttttgctg attggatctc tttcacattc cttcacctca cctgattata tcatggaata
    20821 ggtgcttgag cttgtttcat cctaaaaaaa tcggtatcat tgatttataa aacaggaaag
    20881 aaaggtgacg aacatgcgca aagttaaaat gaatgtccaa ggacgtgtcc agggcgtggg
    20941 atttcgctat atgaccaaga tggttgctga tcagttaggc gtgaccggaa cggtaaaaaa
    21001 cgaagacgac ggctcagtca caatcgaagc aatcggaaat gacgacatca tccaaaaatt
    21061 tatcgaagaa gtcaagaagt ctccgagccc aagtggacga gtgcagtatg tggatgtcca
    21121 agaggatccg atgattgagg aacgaaagaa atttgatgtg gtgggataag gttgtgaaaa
    21181 ggtcgctgag ttttgagtat taaggaaaat tttgtgaaaa tagctttcca catttttgca
    21241 taaatttgac ttaataccga gaaactgact tttgaacacc gtcgatcagg ttgcgaaaaa
    21301 gctgatctgc atcaagcatt aaggactggt tgtgaaaaag tcgcttagtt ttgagtatta
    21361 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttgactt aataccaaga
    21421 aattgacttt tgagcaccgt cgatcaggtt gtttttgagg cgctttgcct caagtattaa
    21481 agataggtag taaaaaagct gatctataac ttgcaataaa tttgcttaac ccatattttc
    21541 cagacagaac aaaaaagttt ttagtttttt aaataagaaa gaaacacttg gaatcactct
    21601 tttctttcca agtatttctt tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa
    21661 aaagaaagaa tatttatatg aaatttctat gaagttgatt gaattccatg cgctttttta
    21721 gtatagttaa gtttgtgtaa aaattataga agaggaagta ttctatgaat aaatggaaaa
    21781 actggttatt gggatccgga cttgtcagta tcctgctctt tttatctggc tgtgtgcgga
    21841 tggattcaaa cggaaaccct gacacttcag gaatcgttta tcgagtactc gtccatccta
    21901 tgggccaagc gattacatat ctagttgaga atttcaattg gtcttacggc tgggcagtta
    21961 ttgcaatgac tgtgatcgtt cgtatcatca tcttgccact aggtatcagt caatcgaaaa
    22021 aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa gcagcacagc
    22081 aaaagctaaa agctgcaaca acacgtgaag aacaaatggc agcacaagca gaaatgcagc
    22141 aagtatatcg cgaaaacgga ttgagtatga ccggcggaat cggctgtctg cctctattga
    22201 tccaaatgcc gatcttctct gccttgtatt ttacggctcg ctacacagaa ggtatccgtg
    22261 aatcatcttt ttacggcatc gatctaggct caccaagtat ggtgcttgtg gccatcgcag
    22321 gggtcgctta tctgcttcaa ggatatattt caacaatcgg tattcctgaa gaacagaaaa
    22381 agacgatgcg tacgatgctg atcgtctctc ctgctatgat cgtcttcatg tcgatcagtg
    22441 cgccagccgg tgttacactt tactgggtag tcggtggtgt gttcagctgt ctgcaaacat
    22501 tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac
    22561 aaaacccgcc aaaacaagtc gtcactccaa gaaaagacgt gacagaatca gcaaagccag
    22621 ctgacaacaa acaagcaaca aaacaaatca aacaaccaaa aaacagtaca aataacaaag
    22681 gccgcaacgc aggaaagcag cagaagcgga agtagatagt aaataagagt gtaataaaaa
    22741 ttcattaaca gttttagtaa ttaaaaatat cgaccaagat ttttgaaaaa gaaatctggt
    22801 cgatattttt atttaagtga atgtttaatt tgaatcatcg attgatcgct atctaattgc
    22861 agcagcatct ttttatgaag aaacaacttt ttcttatcta tcaacgtaga caggtgttgc
    22921 tgtttcttct aatagtgtac ttaattcttt aatattttgt tttccttctt ctaacatcca
    22981 actactcgct ttttcttcgc cttcggtaac aaaaggtgct actcccttcg cccatgttgc
    23041 tcgtccacat aagacaccat tgaatgtcga acctgattgt tttgcaaaac gcaatgtatc
    23101 ttgaaatagt gcagcgctga ctcccgcact aaaaaaaata aacggttgtt cagttacttt
    23161 cgattgttca tagaaataag tggatgcctc tgcttgacta taacaagtct cttgcccata
    23221 tcctttcaca aatctcatat tcactggtac ttctactttc aatacatcca cttgataacg
    23281 ttcttttgaa aattctcgca tggcttcaat cactttatgc ggttttactt ttgcataggc
    23341 aagagaagac ggatcgctaa tagttgcatc ataagaaagg atctctaaaa agaaaggcat
    23401 accttctgct tcgcattctg aatcaatccg ttctataact atttgttttt gcacattaat
    23461 ctctactgat tcatcaacgt catagtacaa taaaaattta caagcatccg cacccacttc
    23521 ttttaacctt ttcactgacc aactattcag taaatctggt agtcggcccg gcattttcgc
    23581 atcatagccg gttttttcat aagctagcaa caagccacat tctttctctc gaagcgctgc
    23641 tgcaggcaga ccatattctg gatcgagtaa aatagcggaa gcatacggag tcaggtactg
    23701 agaaaccatc tgtttaaatt ggatgatatc ttctgatgta gcttctttat attggttgat
    23761 catttttttc aaagcaccac gctggtcgat tgctaacgcg ctaataattc cagcttcatc
    23821 cgtcaatcgc ttcaaacatt cctgtttttc tatactcatt ttcttcatat atcttcacct
    23881 atttctttag ttaatattgt ggctatttct gtttttgatt ttgctgctct caactgttct
    23941 gtaacctctt ttcgcatcaa tgatcgtgca acgagagaca ataatttcaa atgagtcgtt
    24001 cctgcttctg aatctgggat aaataacgca aaaataaaat caactgactg tctatctaaa
    24061 ctgttccact caatcccttt gttcagtgtg acgataatca aactaggttc gttgattgtt
    24121 tgagacttcg catgagggat agcaaaacct gacattattc ctgtagtacc ttctttttct
    24181 ctttctagaa gttttcgata tacttctttt tcgtcatgag cgattcctaa acagaatgtt
    24241 tgctttgcta aaaacttaaa tgcttcttct tgactatcta atggctgttg taaaaaaatg
    24301 ttctcttcat gaatgattgt catgataagc ctcctgctct tccttcttcg ataactgtac
    24361 gatttgctgc tttattatcc atcttcttgt ctttatcagt tactcttttc ttttggatca
    24421 aacctagtat cactccacca acgatcgaac caataagaat cgctgtaacc cattggattt
    24481 ttcccgtcac tacaggtaac actaaaaaac cgccatgtgg ggctggtact tggacaccaa
    24541 acagataggt caagacagca gcaattgatg agcctaacat catcgttggt aatactcgga
    24601 tcgggtcttt cgcagcaaac ggaatagctc cctccgtaat atgagttgat cctaaaataa
    24661 aattaaccaa acctgcatta cgatcattag tatcaaaata tttaccaaac aataaagttg
    24721 caaaaccagt aatcaacggt ggtgcaatgc aagctactga cacgccagcc ataaaagtcg
    24781 tgtttccttg tgccaaaaga gctgtacctg ttacatatgc cgccttatta atcggtcctc
    24841 ccatatcaaa cgcacacatg caaccaacaa tcacgccaag aacaagtgga ttcgaatgtt
    24901 caaatccagc taaaaagctc atcattcctt catttatagc cttcattgga cccgccagca
    24961 aagacatcac gatgccgata aacactacac caataactgg atataagaag attgccttca
    25021 agccatctag ttgtttaggt aaaattgtca gtagcttttg caaaaataag ataaaataac
    25081 ctgctaagaa cccagctaaa atacctccta aaaatccagt accgccactc atggccacga
    25141 ttccaccaac aaatccaacg actaaccctg gacgtttcgc gatcccttct gcaatataag
    25201 ctgctaaaac tggaaccatc atccccattg ctgcatcgcc aaccgttttt aacatcgccg
    25261 ctgtagcatt gtattgtgca tggtctggat caaaagaata gattccccaa acggcaaatg
    25321 aaatagcaat caatactccg ccagctacca cgaaaggcaa catatgagag acgccattca
    25381 ttaaattttt atagatgctg cgaccaaaat tagcaccact tatcgtttct gattctaaag
    25441 tatcaacagc ttcattatct gctaagatgg tcccttttcc tgccaatgct tcgtcaatca
    25501 gccgttgtgc atccttgatt cctgtactga cagaaacatc aatgactcgt tttcccgcaa
    25561 atctttctga atggacatct ttgtctgcgg caataatgac agcatctgcc tgtttgattt
    25621 cttcagccgt taattcattc tcgatcccaa tttgtccatg ggtctccact ttgatcgtga
    25681 tgcctttttt tctagctgct tgctctaatc cctcttgcgc catgtaggtg tgtgcaattc
    25741 ctgtcggaca accggttgct gcaattagtt gatattttcc catctttcga acctctcttc
    25801 cttgattttg atttgtttac ttaattcctc cacatccaaa aaatcagtca gtcctttacg
    25861 aaaagcagta gagctgcctg ctgctatgct tttacgtaac gtttcatcaa gcggtcgtcg
    25921 attcatgtat cctactaaaa aagtgcctaa catggcatca cctgcacatg ctgtattgac
    25981 aacttttcct tttggcgagt ttcctcgaat caccctgtct ttcgtaaaaa gaatcgctcc
    26041 tttctcacca agggataaca aaacattttc tgcacctgac tctaccagtc gttgtccata
    26101 aacaaagtaa tcttcatctg taaccatttt tcgtccgaac caagaagaaa gttcttcctc
    26161 attcggtttc agcaaaaatg gtcgataggg caaacaaccc atgacctctt gatcactgct
    26221 atcaataatc aaatgaatct ccttttcctg acaaatccga ctgatttcca ctaaaacctt
    26281 cggagacatt cctcttggta aactcccaga aacacaaaga taatcctctg cttgtaatgc
    26341 tcttatttga tttaaaaaat tatggacagc tgcctgcggt atttcaggtc cttgattcac
    26401 taatttgtat tcttctctcg tttgattgac ttgtgtaaat acattgatac gcgtcatacc
    26461 aggcacttca ataaactctg tttggatttg tttcttctgt aaatagtctt ctatatattt
    26521 tcctgtgaaa ccagcagaaa aacctaatgc cgtacttggg aaatctagca tctttaatac
    26581 tagtgacaca tttacccctt ttccgtttgc ctgaatgtca tcctcctttg tacggtttac
    26641 cataaaagga tgcatctcct ctgtttcaat aaatagatca attgccaaat tcatcgtgca
    26701 tgtgtagata gacataacca atgacctcct tgaatctatt atgaaatacg cgaaagagaa
    26761 aatgttttct taatcgttcc aacacaaaga aaacctttac atttataaac acatcgtgct
    26821 attttttaac taacttttag tgaaagaagg aaagaatatg gatcacaaat taagtgaaac
    26881 agaacagtac ctctggaact ttatcgaaca ccacatccta gagatcccta actactctat
    26941 cgtgaaacta agtgaacaag caaatgtctc aacagccacc attgtgcgga caatgaagaa
    27001 aaaaggctat gaaggattta cttcgtttaa gcaccatcta aaagaaggag aaaacaagaa
    27061 catcaatttt tctttcttag ataaagtaga caaaggtatc cgtcgagcca ttctaaaaaa
    27121 tgaacaagaa gtggtacgta cgatcaatat gttggaaatc gggaacattg aagatgcaat
    27181 acaaagaatc aaatttactg accgggtttt tgtctttgcc cgaggatttt cagaaatgat
    27241 tggtcaagag atgctcgtaa aacttcaatt aacaggaaaa aattgtcaaa tgcatactga
    27301 tccagagatt atcaaaagca tcagtcggaa actaactaag aaagacagcg ttatttttgt
    27361 ttctttgaac ggcgagacaa aagaactcgt caccgccgca aagaactgct atgatgcaga
    27421 gattggttcg attctagtta cagccaatca ttcctctaca ctcaaagatt attgcgagat
    27481 aaatctcgta ggtttcaaat cagaaggttc ttatttccct gattatgaag tacgttcacg
    27541 cttgcctctc cagatcatcg cgagaatcct gcttgacgcc tatgtattgc gtatgaggga
    27601 gaattgagaa gcagtttatt atggttggtt acagtttaaa acatcacatt ttttattctt
    27661 tatattgtat ggactcacgt aaacttttta tgtataaagt tattca
//
LOCUS       DAGCWU010000020        26104 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.020, whole genome shotgun sequence.
ACCESSION   DAGCWU010000020 DAGCWU010000000
VERSION     DAGCWU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.020"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            110..1114
                     /locus_tag="MBZ71_000708"
     CDS             110..1114
                     /locus_tag="MBZ71_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428681.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1115..2059
                     /locus_tag="MBZ71_000709"
     CDS             1115..2059
                     /locus_tag="MBZ71_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428682.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2059..3021
                     /locus_tag="MBZ71_000710"
     CDS             2059..3021
                     /locus_tag="MBZ71_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428683.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3048..3962
                     /locus_tag="MBZ71_000711"
     CDS             3048..3962
                     /locus_tag="MBZ71_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428684.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3988..5769
                     /locus_tag="MBZ71_000712"
     CDS             3988..5769
                     /locus_tag="MBZ71_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4428685.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6117..6803
                     /locus_tag="MBZ71_000713"
     CDS             6117..6803
                     /locus_tag="MBZ71_000713"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBT4428686.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6823..10404
                     /gene="smc"
                     /locus_tag="MBZ71_000714"
     CDS             6823..10404
                     /gene="smc"
                     /locus_tag="MBZ71_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBT4428687.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10413..11222
                     /locus_tag="MBZ71_000715"
     CDS             10413..11222
                     /locus_tag="MBZ71_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4428688.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11233..12231
                     /gene="ftsY"
                     /locus_tag="MBZ71_000716"
     CDS             11233..12231
                     /gene="ftsY"
                     /locus_tag="MBZ71_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBT4428689.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12418..14685
                     /gene="gshAB"
                     /locus_tag="MBZ71_000717"
     CDS             12418..14685
                     /gene="gshAB"
                     /locus_tag="MBZ71_000717"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBT4428690.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14774..15226)
                     /locus_tag="MBZ71_000718"
     CDS             complement(14774..15226)
                     /locus_tag="MBZ71_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBT4428691.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15376..16326
                     /locus_tag="MBZ71_000719"
     CDS             15376..16326
                     /locus_tag="MBZ71_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBT4428692.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16319..17278
                     /locus_tag="MBZ71_000720"
     CDS             16319..17278
                     /locus_tag="MBZ71_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBT4428693.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17275..18030
                     /locus_tag="MBZ71_000721"
     CDS             17275..18030
                     /locus_tag="MBZ71_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4428694.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18053..19006
                     /locus_tag="MBZ71_000722"
     CDS             18053..19006
                     /locus_tag="MBZ71_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4428695.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19650..19946)
                     /locus_tag="MBZ71_000723"
     CDS             complement(19650..19946)
                     /locus_tag="MBZ71_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBT4428696.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20301..20657
                     /locus_tag="MBZ71_000724"
     CDS             20301..20657
                     /locus_tag="MBZ71_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4428697.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20667..21566
                     /locus_tag="MBZ71_000725"
     CDS             20667..21566
                     /locus_tag="MBZ71_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBT4428698.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22083..22814
                     /locus_tag="MBZ71_000726"
     CDS             22083..22814
                     /locus_tag="MBZ71_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBT4428699.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22959..24986
                     /locus_tag="MBZ71_000727"
     CDS             22959..24986
                     /locus_tag="MBZ71_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBT4428700.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            25094..>26104
                     /locus_tag="MBZ71_000728"
     CDS             25094..>26104
                     /locus_tag="MBZ71_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBT4428701.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIY"
BASE COUNT         8712 a         4561 c         5543 g         7288 t
ORIGIN      
        1 ttcattacac aaagataaga ataacctttg ttataaaacg atttattctg tgtaatgtcc
       61 ttttttatta agaaaaactt ccatttagga agcagagagg ggtattcgat tgtccgataa
      121 ccaattatta gaagttcaga acctacatac aggattccga ctcaaagatg actattacga
      181 tgcagtagat gacgtttcat ttactttaga caaaaacgag attttagcaa tcgttgggga
      241 atctgggtgc gggaaaagta ccttggctac tacgattatg ggattacttg atcctaataa
      301 cacaaaaatt acaggtgaga ttttatataa cgatctgaat ttaatcgacc tgaacgaaac
      361 gctctataat aaaatccgcg gaaatgatat cggaatgatt ttccaagatc cgttgtctgc
      421 attaaatcct ctcatgagaa tcgaagacca gatcaaagaa ggactttcct atcacacaaa
      481 gatgaatgca gaacagcgtc aagctcgtgc actagaattg ctagaacaag tagggattcc
      541 taatcctgcg agagttggac gccaatatcc ccatgaacta tcaggcggta tgagacaacg
      601 tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc gtcattgcag atgaaccgac
      661 gacagcccta gatgtaacca tccaagcaca gattcttgat ttactgaaag accttcagga
      721 agaaacaaaa acagggatca tcttgatcac tcatgactta ggagtcgtag cagaaatggc
      781 tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa gtagcaggtg taaaagaact
      841 gtttgatcat cctaagcatc cttatacacg ctctttactg aactctatac cacaagaagg
      901 aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt ccttctctga aaaacatgcc
      961 tcgtaaaggt tgtcggtttg caccacgtat tccatggata ccagcaagcg atcatgaaga
     1021 agagcctatc cttcatgaag tcgcaccgaa ccacttggta cgttgctcct gctataagca
     1081 tttccacttt agagacgaaa aaggagaggt gtagatgaca gaattaatca caatcaaaga
     1141 cctgaaagtt cattacccta ttcgcagtgg attcttcaat cgagtaacgg atcatgtata
     1201 tgctgtagat ggtgtggatt ttattatcga aaaaggaaag acatatggtt tagtgggaga
     1261 gtctggctct ggaaaatcta ctacgggaaa agccgttgtg ggattagaaa aggtaacagc
     1321 cggacaaatc atgtatgaag gaaaagatgt aacgaagcga agcaaccgca aaaaaatggg
     1381 ttataacaag gatgtgcaga tgatcttcca agattcaatg tccagtttga atcctaaaaa
     1441 gcgagtgttg gatatcattg ctgaacccat ccgaaatttt gaacggttaa gcgatcaaga
     1501 agaaaagaaa aaggtaaaag gactgctaga tatcgttggg atgccggaag atgcgctata
     1561 taaatatcct catgaatttt caggcggaca acgtcaacgt ctaggagtag cccgtgctgt
     1621 tgctaccaat ccaaaactga tcgtagccga tgaacctgta tctgctctgg atctatctgt
     1681 tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa gaatacggcc tcagctattt
     1741 attcatttca catgatttag gtgtagtaaa acacatgtgc gacaatatcg caattatgta
     1801 caaaggtcga tttgtagaaa tcggtacaag agaagacatt tataatgacc cgcgacatat
     1861 ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat gtagaaaata gggaaagcca
     1921 caaagaaaat cgacgaagag tagaacaaga gtatatccaa aaccaaaaag aatactatga
     1981 tgcgactggt cgagtctacg atttgcgtac gatcacaact acacacaaag tggcgttgaa
     2041 agatggaggt gcaagctaat gtggaaaacg attctgcgac gtgttttatt gatgatcccg
     2101 caagttatta ttttaagtgt tctgatcttc ttattagccc aagcgatgcc gggggatcca
     2161 tttaccggtt tgatcaatcc aaatcaggat ccggcagtga tcgaacagat gcgacaagca
     2221 gctggcttga atgacccatg gtatgtccag tacttccgtt ggatcggaaa tgctttgcaa
     2281 ggcgattttg gtcaaagttt cttatacaaa cgctctgttg ctgacttgat cggcgaacgg
     2341 atcatcaaca ctttatatct atcgatcttg acagttgtcc ttacttattt gattgctgtg
     2401 cctctgggga tgcttgctgg ccggtatcaa aattcaatct tggataagat tgtcgtaatc
     2461 tacaacttct ttagttttgc tgttccttta tttatcttcg gcttgatcat gctatttgtt
     2521 tttggttatc gtttaggctg gttcccaaca agtggatcac aaacactagg tttgcaaggt
     2581 ggatttatca ctcaatggat agaccgtttg aaatatattc tgttaccatc aattacccaa
     2641 gcatttttgg cgactgccgt cacaatccaa tatctgcgaa gtgaagtaat cgattcgaaa
     2701 tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac cgacgaataa agtattcagt
     2761 cgacatattt tccgaaatgc agcgttgccg atggcttctc aaatggggta tgaaatcact
     2821 tcgttgattg ctggttctgt agttatcgaa aagatttttg gttatccggg agtcggaaaa
     2881 ctatttattg attccatcgg ccaacgggac tataccgtga tcacatcctt agtattgatt
     2941 ttaggaatcg cgacgctagt ggggacatta ctttcagata tcatcatgag tatcgttgac
     3001 ccgcgtattc ggattcaata aaaccaatca gaaaggagaa tggatcgatg agtgaaaaaa
     3061 acaatacagc agttcaagaa agtattcctc ctatggggat acgaatgatt gctcgagaat
     3121 ttaaaaaaga taaaattgct attttttcat taggtttgct agtggtttta ttattagtga
     3181 tctttatcgg tgcgatgctg accgatcaag acaaagtgat gtatgttagt atcttcgaca
     3241 aatacgctgc accaggaaca gtttcgacac aaggacaaaa gttcattctg ggagcagatg
     3301 aaggcggacg tgatgtatta ggacaattga tcataggtgc tagaaactct gttacgatcg
     3361 gatttgcgat cacgatcctt acttcgatca tcggggttgg attagggatc atctctggct
     3421 actacggagg gatcattgac aacatcttga tgcggatcgt tgattttatc atgattctac
     3481 cgatcatgct gatcattatc gtcttcgtat cgattatttc cagatacaat gtctggtcct
     3541 ttatattcat catgagtgca ttttattggg tagctaaagc ccgattattc cgaagcaaga
     3601 cattatctga agcaagaaga gattatgtca gtgcctcaaa aacaatggga accaatgatt
     3661 ttaaaatcat gttccgcgaa atcatgccga atctaagttc attaatcatt actaatttga
     3721 cgatgaactt tgcggcaaac atcggtatcg agacaacttt gactttctta ggtttcggct
     3781 tgccggcaaa tgtacctagt ttaggtacat tgatcggtta tgcaagtaac ggagaagtct
     3841 tagtgaatcg gcaatggatc tggttacccg catcaatcct tattttagtt ttgatgttga
     3901 gtatcaatta tgttggacaa gcattcaagc gttctgcaga tgcgcgccaa cggttaggct
     3961 agtatagaag cgaaggggga aataggaatg aagaaaacga tttttggatt tgcagcgctg
     4021 atgtctgctg tcgttcttgc agcatgcggg ggcggaggta cagatacctc tggaaacaac
     4081 tctggaggca acggcaataa caaagaggaa ttaacttttc caattgcaac aaaaaataat
     4141 gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg caacggacag tcagtttaaa
     4201 ggattgttcc agtgggagtt ttaccaagat tcatatgatg cagcttttat ggctccttct
     4261 catgaaccgc tatttgcaaa cgatgacgat ttcagaatca cagacgacgg agctgcaacg
     4321 cttgatctag atgaagatac gaaaaaagcg acaatcaaag taaaagacaa cgtcaaatgg
     4381 tcagatggtg aagatgtaac ggcggacgat gtgattttcc cttacgaaat cattgggaat
     4441 aaagattaca ctggtatccg ttatgacgac actttccgta acatcgtagg tatggaagaa
     4501 tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa aaatagacga caaaacagtt
     4561 gaaatcgaat ataaagaagt aaacccaagc atgcttcaat cgggtggtgg tatctggtca
     4621 tatgcagcac caaaacatac gttagaaggt gtagcaatca aagatatgga atcaagcgat
     4681 caagtacgta aaaatccagt gacttttggc ccttattaca tgagtaatat cgttgcagga
     4741 gaatcagtag aatacttgcc aaacgagcac tactggaatg gtacgcctaa attgaaaaag
     4801 atcacgatga aatctgtacc gatggcaagt gcaactgaag cattgaactc taaattatat
     4861 gatatgattt tccaaatgcc aacagatact tacgatacct ataaagaaat tgctggctat
     4921 acaaacttag gccgtgaaca aacttcttat acttatctag gattcaagtt aggtaaatgg
     4981 aatgcagata caaagagtgt ggaatacaat ccagattcaa aaatggctga taaatcatta
     5041 cgtcaagcaa tgggctatgc attagataat aatgcagtag gtgaacgttt ctatgcaggt
     5101 ttgagaagca atgcgacttc attgattcct ccagtattcc agtcatacca tgatgatgag
     5161 atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac tattagatga tgctggctac
     5221 aaagatgtgg atgacgatgg tttaagagaa gacaaagatg gaaataaact aacaatcaac
     5281 tttgcttcga tgtccggtgg ggaaacagcg caaccattgg cagattacta tatccaacaa
     5341 tggaaagaaa ttggtttgga tgtccaatac acaacaggtc gtctgatcga attcaattct
     5401 ttctatgata aattagaaaa cgatgatcct gaaattgatg tttaccaagc tgcttggaat
     5461 acaggaactg atccaaatcc aagtggacta tggggaccaa atgctgcgtt caactacact
     5521 cgttatgaat cagaagaaaa tacaaaattg atcaacgata tcagttcaag caaagcattt
     5581 gatgaagagt accaaaaaga agcatatcaa aaatggcaac aatatgcatt tgaagaagca
     5641 tttgcgattc caacgttgtt ccgtaatgaa gtattgccag tcaacgaccg tgtcaaagac
     5701 tgggattggt cttatgatgt accaaaccca tgggcaaatg tttctgttac agcagattca
     5761 cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc ctttttcggt tttttgatat
     5821 ttttctgttt atttctctaa tattcaggtc aaggcgctga tttcatgact tctccaataa
     5881 acggtgttca aagtccagtt tctcggtatt aagtcaaatt tatgcaaaaa tatggaaagc
     5941 cattttcaca aaattttttc ttcatactcg aaactaaacg aatttttcac aacttctcgt
     6001 caatcactag gattattaaa gctttactta ccttttgacc gaaccatttc aaatttgaca
     6061 aaaaaccagt ataataagga agtatcgaaa aaatatccat aaaaggaatg tggaaaatgg
     6121 acaaccagct gacaaaagaa ctaaaagaaa actataacat cgtttttcac gatctaaatt
     6181 tgcttgaaca agcttttacc cattcatcct atgtgaatga gcatcgcaat ctgcaattat
     6241 cggataatga gcgacttgaa tttttaggag atgccgtttt agaactgatg gtttctcagt
     6301 atttattccg tttatatcct catttgccag aaggaaaatt gacaaaaaca cgagctgcga
     6361 tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg tcactttgat caatatgttt
     6421 tgttgggcaa gggagaagag aattcaggag gacgtacgcg accagcattg ctttgcgacc
     6481 tattcgaagc ttttttaggc gcgttatatc ttgaccaggg attcgacacg acccatgcat
     6541 tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc tttttcacat gagatggatc
     6601 ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga tgtatcaatc gaataccgat
     6661 tgatcaatga agaaggtcca gctcatgaac gagtattttg gatcgaagtt tatgtagatg
     6721 atcaattgat tggcactgga caagggaaat cgaaaaaatt agcagaacaa gctgcggcgg
     6781 aaaatgcatt ggcagcactg taaaagtaag tgagggaaag ctttgtattt gaaacgaatt
     6841 gaaatagccg gttttaaatc atttgcagac aagacagtca ttgattttga aaatagcgtc
     6901 acagcagtgg ttggaccaaa tggcagcgga aaaagcaata ttacagaggc tatccgctgg
     6961 gtattaggag aacaatctgc taaaagtctg cgaggcggaa aaatgccgga tatcatcttt
     7021 gccggttccg atacccgtaa gcagctgaat attgcagaag tcacagtgat tttagacaat
     7081 agtgatcatt atctgccatt ggagtacaat gagatcagtg taacccgccg ttatcgacgg
     7141 actggggaaa gcgagttttt catcaataaa caaagctgca gattaaaaga tatccaagaa
     7201 ttgttcttag attctggatt aggaaaagaa tcgttctcaa tcatttctca aggaaaagta
     7261 gaagcgattt tcagtagtaa accggaagac cgtcgtggga tctttgaaga agcagctggt
     7321 gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga agctttttga aacagaagac
     7381 aacctcagcc gcgtccaaga catcatccac gaattggaag aacagttaac tcctttggcc
     7441 gcccagagtg aggcagcaaa ggagttttta cggctaaaag aaacactgac tcaaaccgat
     7501 gtctctttga tggttgctga aatcaagaca gcaaaaaaag attgggacaa caaacaagca
     7561 cagcttgcga aattcaatct agaattaggc aaactatctg agtcgatcca agaacaagaa
     7621 tccattttgg cgaagcagcg taaagaaaat gcgcaagccg atcgtctgat cgaaaaaaat
     7681 cagcaagtat tattagatct ttcggaaaaa ctgaagcaga cagaaggaca aaaagatgtt
     7741 ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag aatatcaaac ttcattagca
     7801 gaagcacaaa aaaaggttaa acattttgaa aagcttcaag aaagcttgat gaaagcagca
     7861 gcagaaaaag aaacagagat ccaaaaagct gaagcaaatc tcataaaaac acagcaagaa
     7921 ttagaaaaat accaaaaatc aacgaaagaa ttattggcag aattaagaga tcaatatgtt
     7981 gatttgatgc aagaacaagc agctgtagga aacgaactga agtatctaga acgtcaatat
     8041 atacaagaaa cagcaaaaag caagcagacg ttagccaagc aatcggaagt agaagcatca
     8101 gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa agcaggcgca gctaaaatca
     8161 tctttaactg aaacaaaaga gaagttagaa atgatccaac aaaacgggaa aaagttccaa
     8221 gaagcattag caaaagaaca gcctaaaatg tatcaattaa tgaatcaagt ccagcagcta
     8281 cgtgctcgta agaaaagcct tcaagaaatc caagaaaact attttggttt ttaccaaggg
     8341 gttcgattgg tcttgcagca taaacagcag ctttcgggta ttgtcggagc agtggctgaa
     8401 ctgatcgatg ttcctgcaga tttcacgtta gctatcgaaa cagctttagg tggggcagcc
     8461 cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag ccatcacgta tttgaaacag
     8521 caaagaggag gaagagcaac attccttcca ctgacgacaa tcaaaccaag acaattaccg
     8581 gctcatatat tgacacaagc agcagctgta gaagggttta tagggattgc cagtgaacaa
     8641 gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc tgctaggaac gatcctttta
     8701 gccaaagact taactagtgc caatgcaatc gcgcaaacga tccgctatca atatcgagtg
     8761 gtttccttag aaggtgatgt gatgaatgcc ggaggatcca tgacaggtgg agcgaataaa
     8821 cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat tgaaacagtt gactagtgaa
     8881 tttgaagaag cagataaaca actccaagca caagaaaaaa aagttcagga gctgcaacaa
     8941 gaaacagctc gtttagccga agaacaagaa gttttgcgta cacgaggcga acaattacgt
     9001 tttgaagaac aagaagcgac aaatcagcta caaaacatca taaatgaact tgaacgtttt
     9061 gaaaaagaaa agcaaatctc aacttttgag acacgtgaac tgcagcagtt tatcgaagat
     9121 tacgaaaagc aaacgaacga attgaaagac aaacaaacag atcttgaatc gcaacgacaa
     9181 aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg accagatgga agccagaaga
     9241 gcacaagtac aaagtcaaaa agcacaagaa caagcagatc ttgctgtctt gaaagagcag
     9301 tttaatcatc tgcagatcca gctccgcggt gcccgagtcc aaaaagcaga ggcaaccgaa
     9361 cgtcaagaag cgatagaaaa gcagttggcg acattgacag cagacttttc tgatcatgaa
     9421 gtcacagaag aaagtttgga aaaacaaatt aacgaattat ctgctcagcg agaaacatta
     9481 aaagccgagt tggcaaaagc caaagagcag cgagatagaa ggcaaaagga gatcgataag
     9541 cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag cacgactgtc ggaacaatcc
     9601 aaattagaag tccaaaaaga ccgagctgaa atgattttag ataatcattt gacctatctg
     9661 cagtcagaat atcagctgac atttgagaaa gcttcccaag actatcaaga aacaaccgat
     9721 atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac agattgaaaa actgggacca
     9781 gtaaacttaa attcaatcga acaatacgaa caagtcagtg aaagacatac attcttagca
     9841 acacagcgag atgatctgtt agcagcgaaa aaccaattgt ttgaaacgat ggatgagatg
     9901 gatgatgaag tgcggacaag attcaaagaa gtatttgaag ctatccgtca agaattcaaa
     9961 attgtctttc caaatatgtt tggcggcggt aaagctgaat tagtactgac ggaaccaact
    10021 gatttgctga aaacaggtat tgagattgaa gtacaacctc ctggcaaaaa actgcaaagt
    10081 ctaagtttgc tttctggagg agaacgagca cttacagcca tcgcgttgct cttttccatt
    10141 atccgtgtct gtcctgttcc gttttgtatc ttagatgagg tagaagcagc gttagatgaa
    10201 gccaacgtga aacgttttgg tcgttacttg agcgatttcc aagatgacac acaatttatc
    10261 gtagtcacac accgcaaagg aacgatggtc gcagcagatg tactgtacgg agtgacgatg
    10321 caggaatccg gtgtatcaaa aatcgtttct gtacggatgg aagatatcaa tgaagaggcg
    10381 aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa attagtagca atcgatttgg
    10441 acggtacatt attaaatgaa caaaagctga tttcagatga gaataaacaa gcattagcac
    10501 aagcaaaaca gcaaggggtc aagatcgttc tatgtacagg acgcccctta gctgcgatgg
    10561 cgcattatct gcaagaatta ggactggtag atgaaggtga ctacagcatc actttcaacg
    10621 gcggtttggt acaaaaaaat gatactggtg aaatcatgga aaagaaagtg atggaagtat
    10681 cagatatcca acgcttatac aaactggctc agcaattgga tctgccacta gatgttttgt
    10741 ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa gcaatccttg tacaatgtgt
    10801 tgaataagct gctgcaattc caaccagcgg atataagcga tattacagat gaatttgtgc
    10861 ttaataaggc agtaatcgct tatgaccaag aagaattaga tcctaaaatc aaagaaatac
    10921 cagcagaatt ccatgaaatg tatgagatca tcaaaacaag aagtatgctt ttggaattta
    10981 tgccaaaagg tgttacaaaa gcttatggta tttctttatt ggcaaaagat cttggtttga
    11041 aacaagaaga aatcatggcg atcggtgatg aggaaaatga tttaccgatg atccaatatg
    11101 ctggactagg cgtagcgatg gagaatgctg tgcctttcgt caaagaagca gcagatttcg
    11161 tgacagccag caatgaagaa aatggcgtag ccaaagtgat ccaaaaattt gtattagagt
    11221 agggggagtc aaatgggatt ttttgataaa atcaagcgtg cttttactgg cgaagagaaa
    11281 acagaagaag ttgaagtaca ggagaagtac gacaagggtt tagaaaagac gcgtaaaaca
    11341 tttggtgaac ggatgaatga gttgtttgcc aattttcgaa cagtcgatga agactttttt
    11401 gaagaattag aagaaacact tatcggtgca gatgtaggtt ttgaaacagc gatacgaatt
    11461 accgaagcat tacgtcaaga agtgaaactc agaaatgcga aaaaatcggc agaagtccaa
    11521 aatgcgatca tcgagaaact tgtggatctc tatgaagcgg aaggaatcaa tgaagtaaat
    11581 gaactaaata tccaagaaga cggactaaca gttattttgt ttgtcggtgt taatggtgta
    11641 gggaaaacaa caagtattgg taaacttgcc catcagttca aaaacgaagg taaaaaagta
    11701 ttgatggcag cagcagatac cttccgcgct ggagcaatcg accagttagt tgtctggggc
    11761 gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggag gcgatccagc agctgtcgta
    11821 ttcgatgctt tggaaagagc aaagacagag caggcagatg ttttactagt tgatactgca
    11881 ggccgactgc aaaataaagt gaacttgatg aacgaattag aaaaaataaa acgagtaatc
    11941 cagcgggaat taccagaagc accccaagaa acacttcttg tattagatgc aaccactggt
    12001 caaaacgcga tggttcaagc aaaacaattc aaagaaacaa cagatgtgac cggtcttgtt
    12061 ttgacaaaat tagatggtac agcaaagggc ggtatcgtac ttgctatccg taatgaattg
    12121 catttaccag tgaaattggt cggtcttggt gaaggaatcg atgacttgga gccgtttgat
    12181 ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa aagaagaata acccatgtct
    12241 aaaaagcagg agctgtgaca aaagttgcag ctccttgttt gaatgattgt ttttaacata
    12301 gctgtgtttt tgatgccttc atttttatac ttatttgtaa gcggttctca aacaatttcg
    12361 ttctttttct tagacttttt gattataatt agattcatat gaattggagg tcacttaatg
    12421 atgaatttta agcaattatt attgcatgtc aatgcgcgcc cttttatcga ccaagctcga
    12481 tttggtatag aacgggaagg acagcgggtt gatcttgcag gaaatctagc aaaaaccgat
    12541 catccagcaa tctttggcga tcgatcctat cacccctata tccaaacaga ttttagcgaa
    12601 acacaaacag agatgatcac ccctgttacc gattctattc ccgaattatt tcagtatctc
    12661 gctgctgttt atgatgtgac tgctcgttct ataccgaaag aagagatgat ctggccatta
    12721 agtatgccac ctgccttacc ggaaaaagac gaagagatca ttattgcaaa attaaaaaat
    12781 ttcgaagatg tcttgtatcg acgttattta gcaaaagaat acgggaagcg aaaacagatg
    12841 gtaagcggta ttcatttcaa ttttgaattt ggcgatgagt tgctaagaac attgttcagc
    12901 catcaggaag aatttcaaga tttttccgaa ttcaaaacag aactttattt gaaaacagcg
    12961 agaaacttta tgcgctatcg ctggatgatc acttatttat tcggtgcttc accaatgagt
    13021 gagaagaact actttttaga tgaatcacat ccgcaagaac ctgttcgcag tattcgcaac
    13081 agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt cctatgcttc tatgaaacag
    13141 tatttagcag atatcgagcg aatgatcgaa gaagggaaac tttcggagga gaaagaattt
    13201 tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag atttagcaac aacaggtgtt
    13261 cgttatattg aattgcggaa tatcgatttg aatccttatg caagattggg tatcaatcca
    13321 gagcaagttc ggttcttaca attattcctg atgtacatgt tatggacaga agaaaaagaa
    13381 gactgtgacc aatgggtagc agaaggtacg actcgaaata acaaagtagc actagaacaa
    13441 ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa ttcttgaagg aatgaagcaa
    13501 atgctggttg aattggattg gttggattct ctttatttag tagaggaagc attgactcaa
    13561 atggatcatc ctgaacaaac gttagcagcg aaactctatc aagaagcaca gctatcgagt
    13621 cagcaagaag ttgctgtggc attgggacat caatattata aagaaagcca tgaacgtccc
    13681 tatcagttgg ccggttttcg cgaaatggaa ctttctactc agatatttat gttcgatgcc
    13741 atccaaaaag gtgtccaagt caaagtatta gatgagtcgg atcaattttt gcgtctgcaa
    13801 ttccaagacc atgtagaata tgtgaaaaat gcaaacatga caagtaagga cagctatatc
    13861 gtcccactta tcatggaaaa caaaacagta acgaaaaaag tcttaaaaga agctgggttc
    13921 cgggtaccag gaggcgcaga attttcatcc atggaagaag cggtaaaagc ttatccgaga
    13981 tttgccgagc aggcatttgt gatcaaacct aaatcaacca attacgggtt aggtatcacg
    14041 attttcaagg aaggagctag tctggaagat tatcaagcag gtttagccat tgcttttcgt
    14101 gaagacagtt cggttttagt agaagaattt atgccgggaa cggaatatcg tttctttgtg
    14161 atcgacggag aagtccaagc catcatgttg cgagtccctg ctaatgttat tggtgacagt
    14221 atccgaacag tgaaagaact tgttgaagaa aaaaatagtg accctttgcg tggaacgaat
    14281 catcgtgcac ctttagaatt gatccaatta ggtgaattag aacaattgat gttgaaagaa
    14341 caagggttga cgatcgaatc agttccccaa gccaatcaaa ttgtttatct aagggagaac
    14401 tcaaatatca gtacaggtgg tgattcgatc gatatgactg atgaattttc tgaagcttac
    14461 aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta agattagtgg tatcgatttg
    14521 attataccag ataaagaaat cgatcctaca actgataaaa aagcatatgg gatcatcgaa
    14581 gcaaatttca atccagccat gcatatgcat gtttatcctt ttgcaggaaa aggcagaaga
    14641 ttgacgatga atgtattgaa attgttatat cctgaagtat tttaaaagaa aaagcttttg
    14701 acagattgtc ctatttgatg tcaatctgat caaaagcttt ttttgcatgt aaaaatttat
    14761 gtgttttttc gttttaaaat aaacgatccc agaagctttt tttctttttt ggtttttctt
    14821 tttcagctgg cgaagagttt tcttctgggt attttttttc gatatcaatc acaggttcat
    14881 ttactgcttc ttttgctgtt aagagcaatc cgaattcctc aggatttgca gtgacgtaat
    14941 cgttgactac agtgaaacgg ctattgtttt tcgttgctag ctggatatat tggttttgga
    15001 cagaaagggg tagagcccca ttcaagagta tggaagcatc tggatgtttt ttgagttctt
    15061 ttaaaaagtt ttctttgttt tttggttgga tcatttcagc aacagtcata ctaagatagc
    15121 agcgttccct gaatgtccct aaatatttat gctgttcttc agggttcact aaaggtgtac
    15181 cataaagtcc cttatccaaa tggcttttta catcatcttg tgccataaaa aataccctcc
    15241 ttcttctctt tgtctttctt acgattatag catattccca ataataccta aaacatatga
    15301 aaaagcggtt gcttttgttc aacgattcac ttaaaataag aatgaaaatc attctcaatt
    15361 agaaaggtgg attggatgcg ctacggacga ctagtagcat tattagttat tttaagtagt
    15421 atttcattgt tcatcggagt atcgaatgta caattgtcag aactgctcca gttagatggg
    15481 caaagcttac aaatcttgct agtgactcgt ttgccacgaa cgatttgttt gatccttgtg
    15541 ggagcgacca gtagtatttg tgggttgatc atgcaacatt tgacccaaaa taaatttgtt
    15601 tcaccaacga ctgcgggcac gatggatagt gcgcgtttag gtattttagt ggctatgatt
    15661 ttcctacctg gtgcatcctt gattgtccga tccttgacag cattttgctt tgcttttgca
    15721 ggaacattgc tttttttatc ccttacgcga ttgttcctcc agaaaaatca attgatgctt
    15781 ccgttgattg gagtcatgtt agggaatatc attggttcag ttgctacttt ttttgcttat
    15841 caattccagt tggtacaaaa tatgacttca tggcttcaag ggaattttgc gactgtgata
    15901 cgaggaaatt atgagttatt gtatgctgtt gttccattgt tgctcttggt ttggctattt
    15961 gcttaccgat ttacgatcat tggtctgggc gaatcgtttg cagtaaatct gggaatagat
    16021 taccagaaga tgcagttttt aggcattggc ttagtatcat tggctagtgc agtcatgttg
    16081 atcatggtag gaagcattcc ttttatcgga gtgatcattc cgaatcttgt ctctagaata
    16141 tatggagatc aagtgcacaa aacaattggc gtaactgctt tagcaggtag tttgttctta
    16201 gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt atgaaatacc agtcagtgtg
    16261 atcgttggaa tcatcggtgg ggctatcttc ctttatcttc tgataagggg acgacgatat
    16321 gcttaaaaaa aagccatcta tttttattgg tatgcttgtt cttgttttat ttgttacagg
    16381 aatgtacttg acgatccata catatggaaa ctggcatttt gcatgtgcat tgaggagtaa
    16441 gaaaattttt gcatttatcc ttgtctcgct agctgtcagc agtgccacaa tcagctttca
    16501 gacaattacg cataaccagt ttttgactcc ttcagtattg ggacttgagt cgttatatgt
    16561 ccttgtccaa acggtccttt tcttctttat cggaggagtg acgatgcttc agcagacagg
    16621 tacagttttt ttcttattga acgttgggct gatggtcggg ctgagtctat tgttattttt
    16681 ctttcttttg aaaaaaatga gagcgaatct ttttttatta ctgatgtcag ggatgatttt
    16741 aggtacgctt tttggtagtg tcagtacctt tttacaagtc atcatggatc cgaatgaata
    16801 tgatttgctt caaggcagat tatttgcaag ctttggcaat atccagagtc agtatctttt
    16861 gcctgcttct ttgatcatta tcggctgctg gtacctgtta tttcgatatc gacatgtgct
    16921 agacgttttc catctcggaa atgaacaagc agtaaacctc gggatagaca cagatcgttt
    16981 tgaatttttg attttatgtt gtgtcagtct tttgacaggg acatcaacgg ctcttgtggg
    17041 tccagttact tttttagggt ttattgtcgc aaatatcagt tatcgtgtct tttctactta
    17101 tcgtcatggt cagctattta tcggaagctt tctggtagct atgctgtttc tgacaggagg
    17161 acagctcgca gtagagcaga ttttcggctg gaatacaaca gtgagtgtcg tcgttgaatt
    17221 tgtcggagga atttattttg tcacaaaatt gattcgcgaa aggaagagta tcctatgatt
    17281 gatttacaaa aggtcaataa actgtaccat ggaaaatcag tggttaaaga tgtacagtta
    17341 acgattcctg aaaagaaatt gactgctttt atcggtccaa atggtgctgg aaagtcaacg
    17401 gtgttgtcga tgatcagtcg tttgatacca aaagatacag gtgaaatcta tttagaccat
    17461 aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa aattatcgat cctacgacaa
    17521 tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag tagaatttgg acgatttcct
    17581 tatacgaaag gaaaattgac gacaaaagac gaggaaatcg ttcaggaatc actggaacac
    17641 cttgggttag aagaaatagc ggatcaatcg attgacactc tttcaggcgg tcaactccaa
    17701 cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt atattttact ggatgaacct
    17761 ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc agattttaaa aaatttagta
    17821 caagaaatcg ggaaaacaat tgttattgtt cttcatgata tcaactttgc tgcaagttat
    17881 gcagacgaaa ttgttgcaat gaaagatgga cagttattta cgcaaggaac gacccaagaa
    17941 atgataaaaa aagatgtatt ggatgaatta tacgaaatgg acatacggat ttgtgagatt
    18001 gaaggccgca gattctgtct ttatttctaa aactaacatt aggagagaag agatgaaaaa
    18061 attagtagta ggtatcttga ttctagcatc atttggttta gcagcatgtg gaaatagcaa
    18121 caacaccagt caggcagaca caaaaaaaag cagcacacaa actgaaacta cattgacgat
    18181 aacagatagt aatggagacc aaatcgaggt gccaaataat ccagaaaaag tcgtcgtatt
    18241 cgacaatggt tctttagata cgatggatgc tcttggtgtt ggagataagg tagttggagc
    18301 agctacaagc agccttcctg agtatctttc atcctataaa aaagtagaat cagcaggtgg
    18361 aataaaagaa ccagacttag aaaaaattaa tcagcttcag ccagatctaa tcatcatttc
    18421 tggccgacaa agagattttc aaaaagatct aagtcaaatt gctccgacca ttttcctttc
    18481 tttagatgcg aaaaatccat gggaatcttt ccagcaaaat gtcactgctt taggggagat
    18541 ctttggtaag caagaagaag ctaaaactca attagaggaa ttatctagtg caatcgatca
    18601 gacgaaaaag aaagcagaag ctactgacaa aaaagcacta gtcacacttg tgaatgaagg
    18661 gcaattatca gcctacggat ctggttctag attcggtttc atccatgatt tatttggctt
    18721 tgaacaagca gatgatcaaa tcgaagcatc cactcacggt caaagtgttt cttatgaata
    18781 tgtgctggaa aaaaatccgg atatcttatt tgtagtagac agaacaaaag ctattggtgg
    18841 agacgacagc aaagatgata tttctgccaa tgaactggta gcacaaacga atgctggtaa
    18901 aaatcagcag atcatctccc ttgaaccaga tgtctggtat ttaagcgggg gcggtttaga
    18961 atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt aaatagcata aataacagag
    19021 gattaaactc tcatactatg taacaataat gtttcttaca ttcttaaata gcaagagcct
    19081 atcctcaaag aagttatcaa aaaccatccg taaaatcgta ttgatgattt tacggatggt
    19141 ttttatttat ttgagaaatc ttttttcaac gtgaattcat tatctacatc atacgggttg
    19201 attctagaat ggggagtggc aaaaaatagt gtgatccatt ttttcagcat caagatcgcc
    19261 tcctttgatt tgaatcaaag gtacaaagcg aaacttagat aaaacttaaa tttagatatt
    19321 tttattttgt accgatctct tctttaaacg atgttccatc tgctgcttct cggcaataag
    19381 tccactagac tagaaaatat gagaagctat tttctgtcca gcgttcttat tactcaaagc
    19441 aaatcgcagt tgtcacaatc tcttctaaac gatgttccat ctgctgcttc tcggcaataa
    19501 gcccactaga ctagaaaata tgagaagcta ttttctgtcc agcgttctta ttactcaaag
    19561 caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa tcagctgacc ttcggtatta
    19621 gctcaaagca ctgatttcac aacctctatt taggcatgtg tctcagcaag cggttgatag
    19681 ttgatgatct gcatttttcg ataaaaatcg gcgggaagct tactccaccg ttttaataat
    19741 tgatagaaat aactctcttc tgctttgatt ttatactcgc ttaatatcaa tccaaaacta
    19801 ttcgtttttt ttgaagctaa ttttttataa atatccaatg ctttggtttt atcttcattg
    19861 acaaagagtg tttttacttc aatataagaa ccctcgatat tttgtgtttc ttcgataaaa
    19921 tagatgtttt ttatatattc cttcatagtg attcctccaa agaagtcttt tatttgataa
    19981 gtttactcta ccgaagagtt gttaataatc cgtgaacaaa aagtaattct ttttccaata
    20041 gttgttaaag gttttttatt ttttatagta ttttttgtta tttaagaaag gaataaggtt
    20101 aaacaaataa caggttgctt tttgggtctt tttccgttca agaaactgaa cgaagaaaag
    20161 ggaaagttgt ctcttttgtg ataagagaaa tcataataaa cgaatgactt actttccaat
    20221 aaattgagaa cagtcagaac cagacaaatt agttgcatct gatgaataaa gtgattattc
    20281 tatgacatga ggtgatacag atgaaattag aagtgacaaa agaagcacaa gaagtattga
    20341 agaataaatt agaagcagac gatcaacttt tattagatat cgaaaacggt gacggtccat
    20401 ttgcagaaag ccaagtcagt tgccaattgg atacggcatt tcgattgatc attgtcaaaa
    20461 aagatacaca aacagatcta agtctttatt ctgtagtggc tgatacacca gttggaccaa
    20521 tcaaaatcaa agacagtgca atactttaca tggatgatcc atccacactt gctttagaac
    20581 caacttataa tagtttacaa ttaaaaggac caagcggctt gagaaaaggc aacttgcaag
    20641 ttgttcgaaa agactagagg gataaaatgg gaaaattggc gatcatcagc gacctacatg
    20701 tggacatcaa taaattcggc gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc
    20761 acaagatcac tcggcttcat ctagccggag atacggctaa taaagtagac cgttgcctag
    20821 ctgttgtcga atttttcgtc aataaaggat tatctacgac atttcatttc ggcaatcacg
    20881 agttggcaga tgtgcccaat gaacagatga tggaccattt tccagaccca cattttttaa
    20941 atgaacggta tattgcttta aatgagcaga ccgttctttt agggatgaat ggctggtacg
    21001 actatcagtt ttccgaattg aaagattacg aggcaatcgt acgaatgaag cgtttgtatt
    21061 ggtatgatcg gttcatccag cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg
    21121 cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg attttatcca
    21181 ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc tgctccttat gagcgttgga
    21241 acaaattaaa tgcattttta ggatcagaat cattcggaga attacttgac cgttattcaa
    21301 atatcaaaca agtagttttt ggccatacgc atcgtcgctt tgaagataaa tccatcggtc
    21361 acacgattta cagctgcaga ccttttggtt attattatga atggcagctg acccgagatt
    21421 ttgtcttgaa agaacagttg ctagacagct atgatcctat gaaattacgt gtcctcttgc
    21481 gtgcccatca cccagctttt caggcctatc aaacacaaca tttgcaagag gaatttgaac
    21541 aagcgatgac aatcgttccg tattgagaca gcattagtaa tgtgtaaaag aaaatacctt
    21601 gtaaaacata aggataagta agagcagtag atccatttat cgtggatatg ctgctttttt
    21661 tgcggaaaat aatcagcgaa tgtttatttg atcggatgat ttgtaactcg cagagtacta
    21721 gatgcaagcg tgtattcttt ttttgaacga taatcacaaa aaaaaatata atatacattt
    21781 tttatttaat tataattaat attgattgaa gtctaggcag tcaagtcaca ttttgataga
    21841 taggaggggg acttacgaat tattttaaag gctatgacag ctgtatttct agattgtgtt
    21901 tgcatcaagt tgcaataaaa actagaaatc aaatcgggct gataatcatt tataactaat
    21961 cgatacatag gaccaaatat aggtctatgt tcttttattt cataagtatc gtaaaacaga
    22021 attttgctat ggattaatcc aatcgactga tatgtttagt aaataaagaa agaggaatgt
    22081 caatgatgaa aaaagaaatc gtattaacag cagcaatgat gttattcagc atggtagcaa
    22141 gtacgacttt tgtttctgca actgaagtat acccaaagga atataacaca gaaggaacga
    22201 ttacatttga agcaggagat gagggtgtaa ctcctcctgt tgatccagag aatcctgatc
    22261 ccaataaacc tgtcgatcca tcagatcctc caagccctgg aacaggaggg gcattatcga
    22321 ttgattatgg atcaaaattc aaatttggga ctcaaaaaat ttctactgcg gataaaacgt
    22381 attatgcagc tgctgatgtg atgaacgatg ggtctagaaa accgacatat gtccaagtga
    22441 cagataggag aagtacctta tctgggtgga aattaagtgt ctctcaacca gaacagttta
    22501 aaactgccag tggggatgaa ctagttggag cacagctgaa atttacgaaa gggcaggctg
    22561 tttctttggt tgatccaaca tatacgccgc aaacggtgaa ttctgaatta acgttgacac
    22621 cgggaggaaa caatacgtta gcaattaatg ctaaatcagg aacaggtgtg gggacttggg
    22681 tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc ggtacagctt tcggtacccg
    22741 gtaaatctgt caagttagcg caacaatatt ccactaaatt agtttggaca ttagaagata
    22801 cacctaacaa ctagtttact gaaaagaact gttaaaaata aatacgtagt catattgtct
    22861 tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa atttgtttgg atacgaatag
    22921 ttcatctagg aatttaacga tcaggaggaa aagatatatt gaaaaagagg aggatcgtgc
    22981 tcattttaag cagtctctta gtgactaata tctttccgat catagggtac tctttggaat
    23041 tgaatcagtt ggagcatgga aatagaagag acgaagaaag caaactcact tcgacattcg
    23101 acagtttaat agaatccaac cagacacaag aaagcagtga ggatttaagt atttcgagta
    23161 gtggagagac cactgacaac gtagaagctg agggttcgga caaacaagga ctagaagatg
    23221 caccaatttc tgcggttgag ctgcctgacg atcccaatat tatttctatc gataaaattt
    23281 ttcaaacacc tatcggtact agcacgtcca tattggaaaa tgggaagtta ctgcagttga
    23341 acccagcaga aaggtctcaa agaggagcta tttggtcaaa taagaagatt agtttactct
    23401 cggattttac atttaaatct tatttatact taggaaatca acgagataga gcgggagatg
    23461 gcatgacctt tacattgact ggcgatccta gaatggctag taatcccagt caagttattg
    23521 gatcgccagg tatgggaatt ggctcatatt ctactcgttc tggccaacct tacattgcga
    23581 atgcgctctc gattgagttt gatacgtata aaaatagtgg ttcttctgat cgaatggatc
    23641 gagagataac tgctgataat agagggcatg gacatgtcgc ttttgtcacg cctaaaccaa
    23701 ataataataa ttattctgga gagcatactg gcgtgacggt agctcctaca tttctttcta
    23761 atggcacatg gcgcttgtta ataattaaat gggacgcgga gcaaagaagt cttacttatg
    23821 atttggaagg tgtcggtcac tcttctcgga ttatacctga tctcattagg cagtttggtt
    23881 ctacagaagt aaattggggg tttacttctt caacaggtac tttttttcaa gaaaatgctg
    23941 tcgctgtcac gcgacttcca tcgaatgtca catcaacagc tgatgtaaaa gtcaacgata
    24001 gtgattatgg ggccatcgct gaagctggag gaaacgataa gataactatc agaaatacat
    24061 tgaaggtcga gggggatttt cttgaatata agaaaatgca gactagtatc gacctcccac
    24121 aagaattaac tattcccgag catatcttga tcaatggaaa tcaaattgat gccaaagata
    24181 tcttgataac cggaaatcga gtcacgctaa atctgacaga ttattttatt gctggagata
    24241 cgttaataat cgaattggag acaactttgc aggaagtaat tcctgaaaaa gtactcacta
    24301 tgcattttga ataccttgaa aacgggaatt tgattgagca atcgaatgaa ataaacctaa
    24361 agatcgcaaa gctgaaggag atagatatcc atgtttatta taaagataaa gacagtcaga
    24421 ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg agagaagtat aaagaaactc
    24481 ctattgatat tgaagggtat gtttttgact cggattcagc gaatacagaa ggcatcttct
    24541 ccgaaaatac agaggatatc tatttttatt acagaaaagg caaactttat ctctcaactg
    24601 ctcctcaata tgtcaatttt ggaaaacata aaatatccag tagaccatta agtgaattcg
    24661 gacagttttc aggtgggttg aaaattatag atgaacgggc ttttggaagt tggaaattac
    24721 aattgaaaca agagaaagtt cttacaaatg gagattttga gcttccagaa gctctttcct
    24781 ttgttacctc tgaaaataca acggttatag ggacatccgc tgttacgatt ttcgaaagtg
    24841 atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc tacagctcat cgtggaatca
    24901 gaatagatat tcctgtagaa aaccaaagaa tcggaacctt tgaggggaaa ctatcttgga
    24961 tacttgcaga tgtcccagga aattaacagg aagagaagaa tataacaaaa ttattgagtt
    25021 tcaagaaggg cacaggtacc ataggctctt gtgtcccaca gagacaagat tttgttatag
    25081 tcttctctta catatgatta aaggagcgat tttcttgaga ccaaaacaac aattatttat
    25141 aacactccta gttattttag gggcactttt taacaatata tcagtgtcag ctagtgcatt
    25201 taatttttca gtgacgccga taacttcaga gaatcaaatc gacaagagaa aaacttactt
    25261 cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa gcggaattga gaaatgatac
    25321 agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca accaccaatt caaatgtgat
    25381 ggttgaatac ggaaaaaacg agattgaaaa agacatgagc ctgatctttg atctggtaga
    25441 ttatgtgagc tatccacaga tagtgacgct aaaacctaaa agtgtccaaa ccgttgcttt
    25501 tcatgtcagg atgccgaatg agcgatttga tggtgtattg gctggtggga tcacattcca
    25561 agaaatccag acggaaaagg accaaacaga aactaaggat caaagcttat cgattgagaa
    25621 tgaatacacc tatattattg ctttgttgat gcaacagaca ctgaacgaag ttgctccgaa
    25681 ccttactctt catgaagtta aaccagatca aatcaatgca agaaatgtca tcttagcaaa
    25741 cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt attgaaacaa aaattacaaa
    25801 aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa ggattgcaga ttgcgccgaa
    25861 tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg ctaaccccag gtgaatatca
    25921 ccttacaatg actgttttag gaaatgaaaa tgaaaatgga aaattttcga gaaaaaaagg
    25981 aaatactacg atcaactata ccaatcaatg ggtatttgaa aaagatttta caatagacgg
    26041 aaaagtagcc aaggaattga acacgacgga tgttacgatt aaaaaagaca ataattggat
    26101 ttat
//
LOCUS       DAGCWU010000021        25216 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.021, whole genome shotgun sequence.
ACCESSION   DAGCWU010000021 DAGCWU010000000
VERSION     DAGCWU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.021"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(8..2389)
                     /locus_tag="MBZ71_000729"
     CDS             complement(8..2389)
                     /locus_tag="MBZ71_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBT4428702.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="MBZ71_000730"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="MBZ71_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBT4428703.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="MBZ71_000731"
     CDS             3090..3629
                     /locus_tag="MBZ71_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBT4428704.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWDEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="MBZ71_000732"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="MBZ71_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBT4428705.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="MBZ71_000733"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="MBZ71_000733"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="MBZ71_000734"
     CDS             4736..5902
                     /locus_tag="MBZ71_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBT4428706.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="MBZ71_000735"
     CDS             5948..7444
                     /locus_tag="MBZ71_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBT4428707.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMEDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="MBZ71_000736"
     CDS             7525..8208
                     /locus_tag="MBZ71_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4428708.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8875..10044
                     /locus_tag="MBZ71_000737"
     CDS             8875..10044
                     /locus_tag="MBZ71_000737"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBT4428709.1"
                     /translation="MYLEKELDQLKEKGLYNTIDILESENGACIIVDGKRMINLASNN
                     YLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGTEAAIAFQ
                     SGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKDLEKKAKE
                     AIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLVTYVDDAHGSGVTGKGAGT
                     VKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSLTPGAAAA
                     ALASITLMQEHPELVEKVWENANYFKEELKKAGYNIGMSETPITPVILGDEKVTQTFS
                     KKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKEMEII"
     gene            10146..11099
                     /locus_tag="MBZ71_000738"
     CDS             10146..11099
                     /locus_tag="MBZ71_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBT4428710.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWDLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDAMTKDMLEKLKEKLVETV"
     gene            11168..12517
                     /locus_tag="MBZ71_000739"
     CDS             11168..12517
                     /locus_tag="MBZ71_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4428711.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETRGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="MBZ71_000740"
     CDS             complement(12699..13022)
                     /locus_tag="MBZ71_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428712.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPQALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="MBZ71_000741"
     CDS             13151..14314
                     /locus_tag="MBZ71_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBT4428713.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKLAFDSGTVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="MBZ71_000742"
     CDS             14384..15733
                     /locus_tag="MBZ71_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBT4428714.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="MBZ71_000743"
     CDS             complement(15778..16209)
                     /locus_tag="MBZ71_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4428715.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMEKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="MBZ71_000744"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="MBZ71_000744"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBT4428716.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDAPTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="MBZ71_000745"
     CDS             19439..20035
                     /locus_tag="MBZ71_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4428717.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..21305
                     /locus_tag="MBZ71_000746"
     CDS             20157..21305
                     /locus_tag="MBZ71_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBT4428718.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFDDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21343..21756)
                     /locus_tag="MBZ71_000747"
     CDS             complement(21343..21756)
                     /locus_tag="MBZ71_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBT4428719.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21835..22266
                     /locus_tag="MBZ71_000748"
     CDS             21835..22266
                     /locus_tag="MBZ71_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBT4428720.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22365..22598)
                     /locus_tag="MBZ71_000749"
     CDS             complement(22365..22598)
                     /locus_tag="MBZ71_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBT4428721.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22780..24450
                     /locus_tag="MBZ71_000750"
     CDS             22780..24450
                     /locus_tag="MBZ71_000750"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBT4428722.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGIIAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNEWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24615..>25216
                     /locus_tag="MBZ71_000751"
     CDS             24615..>25216
                     /locus_tag="MBZ71_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HBT4428723.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSL"
BASE COUNT         8115 a         4436 c         5310 g         7355 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagggt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactggg
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atccttgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgga agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaatttac ttctggaaca
     8281 ctgtttacca agaggtcatg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcatgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaata
     8521 aagatggaag ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atggcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaaagaact aacaatgtat
     8881 ttagagaaag aattggatca actgaaagaa aaggggttat acaatacgat cgacatacta
     8941 gaaagtgaaa atggtgcttg tatcatcgta gacggaaaaa ggatgatcaa tctagcatcc
     9001 aacaattatc ttggatttgc caatcgcgaa gaattgaaaa aagcatgtat tgaagcaacc
     9061 gaaacttatg gtgtaggagc aggagcagtc cgaacgatca atggttcgct gaaaatccat
     9121 cagcaattag aagaaaaaat cgcagaattc aaagggacag aagcagcaat cgcctttcaa
     9181 tctggattca actgcaatat gggcgctatc tctgcagtaa tgacaaaaga agatgctatc
     9241 ttatcggatg aattaaacca tgcatctatt attgacggtt gccgcttatc cggagcaaaa
     9301 atcatccgaa ttaaacatca agatatgaaa gaccttgaga aaaaagcaaa ggaagcgata
     9361 gaaagtaaga aatataaaaa aatcatgtat atcacagatg gtgttttctc catggatgga
     9421 gacattgcac gattgccgga aattattccg attgttgaaa aatatggact ggtcacctac
     9481 gttgatgatg cgcacggctc aggcgtaact ggaaaaggtg caggaaccgt caagcatttt
     9541 ggattaagcg acaaaatcga tatgcaaatg ggcacgttat cgaaagctat aggtgttgtt
     9601 gggggttatg tagctggatc gaaaacacta atcgactggt tgaaagctag aagtcggcca
     9661 ttcctatttt ctacttcgct aactccaggt gcagctgcag ctgcattggc ttcgattact
     9721 ttgatgcaag aacatccgga acttgtcgaa aaagtttggg aaaatgctaa ttattttaaa
     9781 gaagaattga aaaaagcagg ctataacatc ggtatgtctg aaacacctat tactccagtc
     9841 attttgggag acgaaaaagt gactcagact ttctctaaga agctgatcga acatgggatt
     9901 tatgctaaac caatcgttta tccaactgtg cctcttggga caggcaggat cagaaatatg
     9961 ccgactgcgg aacatacgag ggaaatgctt gatgaagcag tagcagttta tcaaaaaata
    10021 ggaaaagaaa tggaaattat ttaactaaat ttagtttttt cgtctattta aaattagaga
    10081 acatgttcac gcatatattg ttaatcatct gaagatagta gtaaaaaaga tcagggagga
    10141 tcatcatgaa aaaagtattg gtgacaggat gtttaggaca aattggttca gagttgatca
    10201 cacgactacg tcaagaacta ggcagtgaaa atgtcattgc gacagatata cgcatgcctg
    10261 aagtcattga gaaaaacgat atctttgaga tcctagatgt aatggattac gaaaaaatgc
    10321 gcagcatagc agaacaatat caagtggaca cattgattca tttggctgct cttttgtcag
    10381 ctgttgcaga agcaaaacca caattagcct gggatttgaa cgtgactggg ctggtcaatg
    10441 cattggaagt cgctagagaa ttccagctga aattttttac gccaagttca atcggcgcgt
    10501 ttggtccaaa tactccaaaa gatcataccc cacaagatac tcttcagcgt cctacaacca
    10561 tgtatggggt aacgaaggta gctggagaac tactatgtga ttattattat gtgaaatatg
    10621 gcgtagatac tcgtggtgtc cgtttccctg gattgatttc ttatgcaacc ttgccaggag
    10681 gcggtacgac agactacgca gtcgatattt attattcagc aatcaaagaa ggaaagtaca
    10741 ctagtttcat tgatcaaggt acatcaatgg atatgatgta tatgccagat gcgatcgatg
    10801 ccattataaa actcatgaat gcagatcctg atcgattggt tcatcgaaat gccttcaata
    10861 tatcggcgat gtcttttgac ccggaagaga tcaaagcatc tattcaaaaa atcataccag
    10921 attttgaaat ggattatgat gtggatcctg tacgtcagtc aatagcagaa tcttggccaa
    10981 attcactaga tgtttcttgt gcgcaagagg aatggggctt ttcgccgaaa tacgacttag
    11041 atgcgatgac aaaggatatg ttggaaaaac tgaaagaaaa attggtagag acggtataaa
    11101 acaagaacca catccaaaaa gttttttata cttttgggat tggctatggt atgaaagggg
    11161 acaagggatg gaagaatatc aagccacgcc agtaaaagaa gtaaaaatag aaaatgaatt
    11221 aaaacgtacg atgggatttt ttactgcttt atcgactgtt atgggcacag tgatcggtgc
    11281 aggggtattt tttaaagccg caagcgtagc agaagttaca ggatccgcca gtttgcatat
    11341 gttttcttgg tttctcggtg gtatgatttc tgtctgtgca ggactaacag gagctgaact
    11401 ggcagctgct attccagaaa caagagggat gattaaatat atcgaacgga tttacggaaa
    11461 tacagcagct tttttattgg gctgggcgca agtcgtgatt tattttccag caaatgtagc
    11521 agccttgtcg atcattttcg gtacacagtt cgtcaatcta tttggtcttt cccagtcaat
    11581 gattgttccc gtagctgtta cagctgctgt atcgatcctg ctgatcaatt ttttaggttc
    11641 taaagctgga ggggcttttc agtcgattac gcttgtctgc aagcttatcc ctttgtttgt
    11701 gatcgtcatt ttcggtttgt tccgacaaga aggagtagac ttccaactgt ttccgatcca
    11761 agcaggagaa aatctttcat ttttctcagc cttgggagcg ggcttgctag caacgatgtt
    11821 cgcatacgac ggttggatac acgtaggaaa tatttcggga gaattgaaaa agccggcgaa
    11881 agatttgcca aaagcaattt ctttaggaat catcggaatc atgatcgttt atttattagt
    11941 aaatgcggtg ttcttaagaa ccgcatctat cgacggagtc gcaggaaaca gcaatgcggc
    12001 aagtgacgtt gcaaaaatga tttttggcgg cttcggtgga agactggtca cagttggtat
    12061 cttgatttct gtttatggaa ccatcaatgg ctataccctt actggaatgc gtctccctta
    12121 tgttatggca aaagaaaaca acctgccttt tagcaagctc tttgctaaac ttcatgataa
    12181 aactaaagtt cctgtagccg caggtatctt agagctagtc atcgctattg gcatgatgat
    12241 gatcggtggt tttgatacac taacggatat gctgatcttt gtcatttgga ttttttatac
    12301 gatggtcttt gttggtgtca ttcttcttcg taaaaaagaa cctgatttgt ttcgaccata
    12361 caaagttcca atgtatccgt ttattccact agtagcaatc attggaggga cttttatcgt
    12421 atcaagcacg cttatcactc agaccttttt agcttcgatg gggatagtat tgacacttgc
    12481 cggtatcccg atttatctct atttaaaacg tagatagcta aaacattcga attttattag
    12541 aactgcactc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacaatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgcctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcttg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgactg ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa cttgcgttcg attcagggac agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tgcaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaaccctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 cgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgttt ctcatgttat caatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt ttttccattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgccaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gattgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaagg aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gctggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gattctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt atcgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgatgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21301 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21361 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21421 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21481 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21541 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21601 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21661 tagctgttct tgtttacctt gatagacaat caagattccg cctccactag gaccagggtt
    21721 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    21781 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    21841 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    21901 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    21961 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcgacg ttcttttatt
    22021 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22081 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22141 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22201 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22261 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22321 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22381 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22441 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22501 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22561 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22621 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22681 gtaattttat gtttttttta ttataaaaaa aggtataatt ggcatatctt aacaaagaag
    22741 atattttgtt gaataaagtt cgtgaaaagg ggtaatcaaa tgaagaatga tctgcaaatt
    22801 tcacaagaaa cagagctaga accgattgtt gaagtggcta gtaaattaga tcttaaggaa
    22861 gacgatttgg aactgtatgg gaaatataag gcgaaaatca atttttctgg aatcaatcga
    22921 tcaaaggata acgctgaggg gcatttgatc ttagttactt ccatcaatcc aacaccagct
    22981 ggcgaaggaa agtcaacgat cactgtaggg ttaggagacg cgctcaataa aatcaataaa
    23041 aaatctgtta ttgcattgcg agaaccctca cttggtcctg tgatgggaat caaaggtggt
    23101 gctgcaggcg gaggatatgc acaggtattg cctatggaag atatcaactt gcattttact
    23161 ggtgacatgc atgctatcac aacagctaat aatgcattat ctgctctttt agataatcat
    23221 attcatcaag gaaatgaatt gaacatcgat gcccgccgag tgatttggaa acgggtagtc
    23281 gatctcaatg atcgtgaact gcgtaaagta attgttggct tgggtgggcc aatccaaggt
    23341 gttccaagag aagatgggtt tgatatcaca gttgccagcg aaatcatggc tatcttgtgt
    23401 cttgcggcag accttgaaga tttaaaggct cgtcttgcaa ggatcgttgt cggctataca
    23461 tttgaccgtc agcctgtgac tgctggagac ttgaaagcag aaggcgctct ggcattactg
    23521 ctgaaagatg caatcaagcc gaatcttgta caaacaattt acggaacacc agcttttgtc
    23581 cacgggggac cgtttgccaa tatcgctcat ggctgcaata gtattttagc taccaagaca
    23641 gcattgagat tagcagatta cgttgtcaca gaggcaggat ttggtgcaga tcttggcgga
    23701 gagaaatttt tagatatcaa agtacctaac ttgaaaaaag cgccggatgc tgtggtgatt
    23761 gtcgccactg tccgtgcatt gaaaatgcat ggcggaatga aaaaagatga acttaaaaat
    23821 gagaatcttg atgctttgaa agtaggtttt gcaaatttaa agcgccatat acgtaatatg
    23881 gaacaatatc agctacctgt gatcgtcgca atcaatgaat tcgtaacaga tacagacaat
    23941 gaactcacac tgttggagca cttgtgtgaa aatcagggga ttattgccaa gagagcgagt
    24001 gtatgggcag atggagcaga aggcggcgtt gatttagcaa aagctgtagt acgattgatt
    24061 gatagaaaag aagctaatta caagccatta taccgcctag aagaaacgat acaagaaaag
    24121 acagaaacaa tcgtcaagaa aatttacgga ggaaatggtg ttgttttcag caaaaaagcg
    24181 caaaaacaaa tagaagagtt tacgaagaat gaatgggaca aactgccgat ttgtatggca
    24241 aaaacacagt attcattttc tgatgatcct tcactgctag gtgcaccgga agatttcacc
    24301 atcacgatcc gcgaaatcat cccaaaatta ggggcaggat ttttagtagc cttgactgga
    24361 gatgttatga cgatgcctgg tttgcctaaa aaacctgctg cattgaatat ggacgtgaca
    24421 aatgacggag aagtgttggg attgttttaa gcttgattat aatctaccgt atactaaata
    24481 actttcatta aacatcgaag gagctgtatt ttgactacag cttccagact gtagacaaac
    24541 tcctaaattg ggagtgttgt ttgcagtttt tttgttttat actcattgta taaactattc
    24601 tgggagtgat tctaatgatg agaaaacaat cgattgaagg tagaaatcaa tttgctatgt
    24661 taacaattga tgatcttgta cctaaagatc acttagttcg aaaaattgat gctgcgattc
    24721 agtttgattt catttatccc attgtagaat ctacgtattc aacctatggt cgtcccagta
    24781 ttgatccagt ggtcttaatt aaattagtat ttatccaata tttatttggt atccgttcga
    24841 tgagacagac aattaaagac gttgaaacaa atgttgctta ccgttggttt ttaggttatt
    24901 cattcgagga tcctatccca cacttctcta cttttggtaa aaactatgtt cgaagattta
    24961 gagaaacaac tttatttgaa gatattttct ctcatattct agaacaagct gtaaaagctg
    25021 gcttcgttac ggaagacaac ctttatattg attctactca tatcaaagca aatgccaata
    25081 aacataagtt cacgaaagag atgacctacg gtcaagcaaa agcttatcaa gatgaattag
    25141 aagatgaaat aaataaagaa cgtattgctg taggaaaacg tccttttaca tgggacacag
    25201 aaagtgagtt gagtct
//
LOCUS       DAGCWU010000022        24555 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.022, whole genome shotgun sequence.
ACCESSION   DAGCWU010000022 DAGCWU010000000
VERSION     DAGCWU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.022"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="MBZ71_000752"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="MBZ71_000752"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBT4428724.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="MBZ71_000753"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="MBZ71_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBT4428725.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="MBZ71_000754"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="MBZ71_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBT4428726.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="MBZ71_000755"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="MBZ71_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBT4428727.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="MBZ71_000756"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="MBZ71_000756"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBT4428728.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="MBZ71_000757"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="MBZ71_000757"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBT4428729.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="MBZ71_000758"
     CDS             complement(8053..8523)
                     /locus_tag="MBZ71_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBT4428730.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="MBZ71_000759"
     CDS             complement(8569..8994)
                     /locus_tag="MBZ71_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBT4428731.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="MBZ71_000760"
     CDS             complement(9061..9330)
                     /locus_tag="MBZ71_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBT4428732.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="MBZ71_000761"
     CDS             complement(9341..10936)
                     /locus_tag="MBZ71_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4428733.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="MBZ71_000762"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="MBZ71_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBT4428734.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="MBZ71_000763"
     CDS             complement(14489..15049)
                     /locus_tag="MBZ71_000763"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBT4428735.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="MBZ71_000764"
     CDS             15402..16376
                     /locus_tag="MBZ71_000764"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBT4428736.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="MBZ71_000765"
     CDS             16546..17946
                     /locus_tag="MBZ71_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBT4428737.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="MBZ71_000766"
     CDS             complement(18024..18746)
                     /locus_tag="MBZ71_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4428738.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="MBZ71_000767"
     CDS             complement(18825..19331)
                     /locus_tag="MBZ71_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBT4428739.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="MBZ71_000768"
     CDS             19454..21253
                     /locus_tag="MBZ71_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBT4428740.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22859)
                     /locus_tag="MBZ71_000769"
     CDS             complement(21321..22859)
                     /locus_tag="MBZ71_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4428741.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     STTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
     gene            complement(23114..23623)
                     /gene="mreD"
                     /locus_tag="MBZ71_000770"
     CDS             complement(23114..23623)
                     /gene="mreD"
                     /locus_tag="MBZ71_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBT4428742.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23627..24481)
                     /gene="mreC"
                     /locus_tag="MBZ71_000771"
     CDS             complement(23627..24481)
                     /gene="mreC"
                     /locus_tag="MBZ71_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBT4428743.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
BASE COUNT         6790 a         5337 c         4459 g         7969 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgattct tctgttgaag attcaggtgt tgttgattct
    21961 tctgttgctg aagattgtgt agctgatgat gattcttcag tcattgaaga ttgtgtagct
    22021 gatgattctt ctgttgctga gctttgtgca gtagatgaag cttgagtagc ttgtgcttca
    22081 gcctctgctt gttcgcgtgc ttctttttca gctttttctt gtgcagcttg ttgacgagct
    22141 tgttctgcta gacgttgttg ttcacgaata cgagcttgct ctgcttcagc ttctgctttt
    22201 tgacggttca agtcagcttt cttgtcttca gcagttgctt gttcagcagc taatgaagtt
    22261 ttcagtacgt tcaaatcagc ttgtttagat aaaagatcgc ctttttgaga ttccaaagca
    22321 gcttggtttt cagctaattc ttttagttta gcatcgtttt cagctttttt gtcttctaca
    22381 gcttttttat cttgtttttg ttgttccatc aagtcgttgt ttgcttttac catcgttgtc
    22441 attgcttgaa cgcgtccaat agcatctgct aatgaatcag cattcaatac agcatcgatg
    22501 tagtttgagc ttgtgttgct tacttgtgct tcgcgtgctt gtttttggat tgtatcttca
    22561 cgtttttcaa tacgttcttg taaatctgca atgtctttta ctaattgtgc agattcttga
    22621 cgcaatgtat cttgttttgc taataggtct tgtgcttgtg tgttgatttc agaaacttgg
    22681 ctttctaagg catcgatttg tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc
    22741 ttttgatctt gctgttgtat ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat
    22801 gctacggcag tcaaggtcat tgaacatacc attactgctg atattaaact ctttttcact
    22861 cttcattcct ccgactggct taatttaata aatatctaaa tatatttaag tctttcttaa
    22921 cgatataaaa attgtaccat agcagtgtgt caacgatata atagttatgt tacaaaaatg
    22981 tttcaaaaca acttttaaaa atgacaaaac tgttcgtttc agaaaatgat catgaaaaag
    23041 ccgcgttaag tctagcattt cttaagataa caacgattct ttttcgcttt cgacctaaaa
    23101 ataaaaaaag aaattattca tcactgaata atttcttaaa gggaaaaatg aataatacaa
    23161 atataatcat gttcaacagc agtgtaggtc ctaaaaacct tgtaataaaa tacgtgttat
    23221 ttactactgc taatttaaaa atcaactgga ccaccatcgt aaacaattca taacccgtga
    23281 caaagatgat catactgaaa aattcagtaa agatgttgac atggataacg tctttcatta
    23341 catacatcaa ccatacaatt aaagggagag ctactgcata gataccgata acgccaatat
    23401 agtaagcgtc atagatagcc ccgagaacaa tcgtggtaat cagtaaataa cgtttttcaa
    23461 acgccatact gcaacataat aatgccaata tcagaaagtg ggcatttgac atataggtgc
    23521 ctttcgacca ctcccctagc attcttgtta aatgaccatc tatcaacatc aaaaagaaca
    23581 aaacgattgg cagatagtac ttcattgttt ctttcttcaa catggactaa tcctccgcta
    23641 gccgttggac aatcgtcaca actgacacgt catacatttc tgcataaggt ttcacataaa
    23701 cttcccgatc cagcccataa ctatctggtt tcgttttaat aaccgtaccg atcggcaagt
    23761 tagctggaga attccctcca agaccggatg tctggacaac atccccttct ttgatatccg
    23821 tatcaccagt taattgggtc accactaagg catggagctt ttcatcatag tttttaagca
    23881 aaccaaacgc ttcgccatta gccgaagata cccgtactgg aaaatgattg gagctttcat
    23941 tagaggatga cagtaattcg attttagacg aagccgtatt gacctcgatc actcggccaa
    24001 tcaatccttt ttgcgacatg actgccatat ttgcttcgat accgtcttta gaacccttgt
    24061 ctacgatcag catgtcctgc caactgtcag gcgaacgagt aatgacattt gccgttactt
    24121 tttcgtaact agtcagcgtc tgattcaact caagttcttt tttcaacgct tcgatttctc
    24181 tctcgtaatt cttgttttgc tgtgaaacgt catcatagct atcgattttt tcttttagcc
    24241 gctcattttc ggcataagtt gaaaaaagat tatggacaga atctactcca tttcgtaccc
    24301 accgtactgg ggcagagatt gttttatcaa ccattgcaac actgtcatta acgatggatt
    24361 ggaagaaatt gggctttcct tcattcgctc gctgcgcagc ggtcacactt agtatcgtca
    24421 ccacgatgat aacaataacc aaggtaataa taatattttt attaggattg aacttcttca
    24481 caaagacacc tcgaattaat tagactcttg tgttcaattt tatcactaaa tgccgtgaaa
    24541 caaaaggtaa gttgc
//
LOCUS       DAGCWU010000023        24300 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.023, whole genome shotgun sequence.
ACCESSION   DAGCWU010000023 DAGCWU010000000
VERSION     DAGCWU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.023"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            32..664
                     /locus_tag="MBZ71_000772"
     CDS             32..664
                     /locus_tag="MBZ71_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428744.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(798..923)
                     /locus_tag="MBZ71_000773"
     CDS             complement(798..923)
                     /locus_tag="MBZ71_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4428745.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1057..1950)
                     /locus_tag="MBZ71_000774"
     CDS             complement(1057..1950)
                     /locus_tag="MBZ71_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBT4428746.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2132..3058)
                     /locus_tag="MBZ71_000775"
     CDS             complement(2132..3058)
                     /locus_tag="MBZ71_000775"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBT4428747.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3143..3904)
                     /gene="pflA"
                     /locus_tag="MBZ71_000776"
     CDS             complement(3143..3904)
                     /gene="pflA"
                     /locus_tag="MBZ71_000776"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBT4428748.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4148..6379)
                     /gene="pflB"
                     /locus_tag="MBZ71_000777"
     CDS             complement(4148..6379)
                     /gene="pflB"
                     /locus_tag="MBZ71_000777"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBT4428749.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6656..9106)
                     /gene="parC"
                     /locus_tag="MBZ71_000778"
     CDS             complement(6656..9106)
                     /gene="parC"
                     /locus_tag="MBZ71_000778"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBT4428750.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9118..11163)
                     /gene="parE"
                     /locus_tag="MBZ71_000779"
     CDS             complement(9118..11163)
                     /gene="parE"
                     /locus_tag="MBZ71_000779"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBT4428751.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11329..11763)
                     /locus_tag="MBZ71_000780"
     CDS             complement(11329..11763)
                     /locus_tag="MBZ71_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBT4428752.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11890..12528
                     /gene="plsY"
                     /locus_tag="MBZ71_000781"
     CDS             11890..12528
                     /gene="plsY"
                     /locus_tag="MBZ71_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBT4428753.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12660..13535)
                     /locus_tag="MBZ71_000782"
     CDS             complement(12660..13535)
                     /locus_tag="MBZ71_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBT4428754.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13617..14408)
                     /gene="codY"
                     /locus_tag="MBZ71_000783"
     CDS             complement(13617..14408)
                     /gene="codY"
                     /locus_tag="MBZ71_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBT4428755.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14426..15826)
                     /gene="hslU"
                     /locus_tag="MBZ71_000784"
     CDS             complement(14426..15826)
                     /gene="hslU"
                     /locus_tag="MBZ71_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBT4428756.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15840..16388)
                     /gene="hslV"
                     /locus_tag="MBZ71_000785"
     CDS             complement(15840..16388)
                     /gene="hslV"
                     /locus_tag="MBZ71_000785"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBT4428757.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16425..17330)
                     /gene="xerC"
                     /locus_tag="MBZ71_000786"
     CDS             complement(16425..17330)
                     /gene="xerC"
                     /locus_tag="MBZ71_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBT4428758.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17528..18904)
                     /gene="trmFO"
                     /locus_tag="MBZ71_000787"
     CDS             complement(17528..18904)
                     /gene="trmFO"
                     /locus_tag="MBZ71_000787"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBT4428759.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18873..20951)
                     /gene="topA"
                     /locus_tag="MBZ71_000788"
     CDS             complement(18873..20951)
                     /gene="topA"
                     /locus_tag="MBZ71_000788"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBT4428760.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21073..21930)
                     /gene="dprA"
                     /locus_tag="MBZ71_000789"
     CDS             complement(21073..21930)
                     /gene="dprA"
                     /locus_tag="MBZ71_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBT4428761.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21986..22753)
                     /locus_tag="MBZ71_000790"
     CDS             complement(21986..22753)
                     /locus_tag="MBZ71_000790"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBT4428762.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22746..23606)
                     /gene="ylqF"
                     /locus_tag="MBZ71_000791"
     CDS             complement(22746..23606)
                     /gene="ylqF"
                     /locus_tag="MBZ71_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBT4428763.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23866..24264
                     /locus_tag="MBZ71_000792"
     CDS             23866..24264
                     /locus_tag="MBZ71_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4428764.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         6385 a         5187 c         4185 g         8543 t
ORIGIN      
        1 tatatactta tcaatttaat ttggagatat tatggaaatt caacgtagaa ccactaattt
       61 tttacattta ggctcttttt ttgcaacttt gacgatctta ttttttctat gttattcata
      121 ccttaagacc tcacatgcgc cttctagtgc attaggctgg ttgcttttgg atcattctga
      181 tatcacagaa gccgaagaaa atccgttttt tttgattttg tcttctcttt gcggcttact
      241 gttttctgtt tatttcggtg cctatttttt tcatcgacat cctggtacgt taaacgaagc
      301 ttcaagcgta ttgattgcat ttaaaacgtt ttctttttta gcaattcttt tttattcgtt
      361 taatcacaat acgcttttct tctttatcat gaatggttta gtcgtgattt tatccatttt
      421 tacttatttt attttttcac tcatttttag ccaattgaaa tgtccgctct tctttcgatt
      481 agttcctgtc acctgtttta tttacagctt gttttccttt tttgtccttg ctttaatgcc
      541 actgaccaac ccttcgacga tccaagtcgc aggaaatctg ttggatatgc tcttcatcgt
      601 cagtctaggt gtatttatgt ggcaaagaaa aaacaaacaa aataaaaagc attttgaagc
      661 ctgactatta cgacttcata aatgcttttt tagttttaaa cacttcaatc taaaagaata
      721 acccaagatt ggataaatta ttaatgactc gccattcgtt tcttagtgat tctggattat
      781 caaatggact tcctaatcta atcagctagt tcttcccata aaatgccata cttgactgaa
      841 tcataatatt gattatttaa aaatctaact tttcttatca ctacttcctt tgtcattcca
      901 catttctcac catttttttg cattcctttg tttccagacc atgtagtgaa tctaataggt
      961 ggtagatagg caaaatactc aaataattct tttaaccata aaggaagaga aaggctcctc
     1021 ctgcaagagt cagtataatt tttgcttact gctctcttag gttttactca ctaaaactgt
     1081 tccatattct ttgatataag aaaatgcatg cggtataaat ttcttgctca gctcagaaaa
     1141 gatttgtcgt ttaagaaagg ccatttcctc cattgtcaat gaaggatcca atgttggtaa
     1201 ttcaatctca aaattgtttt tttcgacaat aggtaattct aatgtgaatg attcttcttc
     1261 ctttatagta ttcgtcacaa gtagagactg atggaaaata ctaggccggc gatttttata
     1321 tttattctta cgtatcctgt caacgataac ttctatttga tcaaaaaagt acgattcatt
     1381 tacgtctcca ataagttcgg ctaattcttt gaatgccttc gttacaggaa gctgattctt
     1441 ctgtacaaaa aagtaattag ccaaagtaag cgggaagtgt tctatagagg aaagtgtgat
     1501 aaaacggtac ttctcatttc ctgcagttgt cctcagcaat tctgtctctc ggctgtttct
     1561 tactccataa aaatacgctt ctttccgttc atcttcgaaa caatacgccc aaataacttc
     1621 tcccatcgct aatttttgtt catcaacaga gagtctcttc aattgttcag ctatggtttc
     1681 agcagccgaa gtggcttgtt gcaagtaatc tttaggatca aatattggaa agttcaaatg
     1741 atatcttcgt tcttccctct tgattaatcc tgcatcgatc aataaatcaa gaaaatggga
     1801 aaatttattt tgcgaaaatt cttttttcaa ttctcttaga atcgtttctt gttctcgatg
     1861 ttcatataga taatcggtca atgaagaaaa aagcggatca tcggtcagtt tttgataaaa
     1921 gttttcctta ttttttgaat aatagttcat atatgttctc ttttctatcc gttttatttt
     1981 acttaaaagg cttttacgta aaaaggagga agaacaagag tcataattga ctgttattct
     2041 cctccatttc ttcggccttt agtatgttct atgtttcttt ttatatcaat cgcttatcca
     2101 ttcattttga atgcttcata ctaccatttg attagctaaa tacagccgtc aattgaggta
     2161 cgacttgttt tttacgtgaa acgacacctt taagcaaagc acggttgttg gtcaaagttg
     2221 tttggaaagc ttcttctact ttttcttttc ctttaccagc aacaagtaat tctgaatcac
     2281 tgtctaagat gtttgtgaca actaatacga acagatcgta gccatttgct ttcatttcat
     2341 cttccattgc tgcttctagc tctgcttgtc gatccatcac ttcattaagg tccactgtat
     2401 tgacttgagc gatacgaacg ttggaactgc ccattgggaa actttttgca tccaaatcaa
     2461 gaagaaccgc agctgattta tcacttagat ttgttcctgc tttcaacatt tcaagtccat
     2521 aagtgtttag atcgacttca gcgattgctg caagttcttt tgcagctgct tcatcttcag
     2581 gcgtacatgt tggtgatttg aacaacaatg tatctgaaat gatagctgag agcatgattc
     2641 ctgcgatttc tttaggaatt gttacttggt tttctttata tagttttaaa ataatcgtac
     2701 ttgtacatcc aactggttct gcacgatagt acaatggatt agctgtttca aagtttgcaa
     2761 tacgatggtg gtctactaca gctaaaatat ttacttcgct gatatctgaa atactttgtt
     2821 ggaattcatt atgatccaca agcatgacat ctgcattttc tgctttagct gactctacta
     2881 ctcgtggtgc agatagctga aagtggtcta atgcgtattg tgtttcttca ttaggtgtac
     2941 ctagagcaac tgcttctgtt tctttaccta attcattttg caagtgtgaa aacgcaatgg
     3001 ctgccccgat tgcatctgta tctggatttt ggtgaccaaa aactaaaatt tttgacatgt
     3061 cattcgctcc tttgtgattt ctcctattta ataatagcaa aaaaacggaa agaagcaata
     3121 cttatcttct ttccgatgcg acctatcgcg ttaaatattt tgtatactct tctgtatgaa
     3181 gcaatcgttt agcattttcg acacgttctt ttgtaggtgg ttcgatccct tctaatggat
     3241 atttcaaacc aagtgtttcc cacttgtatt ttcccatcgt atgataagga agaatctcta
     3301 ctctgtctac atttgacaag gtttgaataa agttgtctag tcggatcaaa tattcatcat
     3361 aatcacttcg ctcaggaact agtacatggc gaatccaaac cggtttgttg atttttgaca
     3421 aatattgagc catttctaaa atattttcat tgtcccaatg cgttagtttt ttatgctctt
     3481 cattatcgat gtgtttgatg tcaaataaca gtaaatctgt atacttcatc aattcttgaa
     3541 aacgtgaaaa gaaaggttct tcatacgtaa aaggttttcc gcatgtatcc aatgtagtat
     3601 gcatcttccg ctcttttgct tttttaaata gttcgatcaa aaaatcgatc tgcaatagag
     3661 gttctccacc actaacagta ataccacctt tatctcccca gtaaggtctg aagcgttctg
     3721 ctttatctaa aagttcatct gctgtgtatt ctttccctcc gccaatattc caagtatctg
     3781 gattgtgaca aaattggcaa cgcatgcggc atccttgcat aaacacgaca aagcgcagtc
     3841 caggtccgtc tactgaaccg aaagtttcga tagaatgaac atagccgatt gttttttctt
     3901 ccatttaagt tcacatcttt tctttgattt cattttaagt acaagttagc tgtcgaagcg
     3961 aagctttctg aacaaaattt ttcgcaattg ccgcttcgtt atgtgtaaaa tttacaggga
     4021 cggaacatac aagtcctgcc cctgtaaatc gtttctttca aataagcata ctgattattc
     4081 gattttgatg cgatggctat tcaacgtcct ttcgacgcta agtcgaaaaa cttttttcac
     4141 gaacttttta cattctgtcg tgtgaagttc tcatcaagac atctaattgt tgttctcttg
     4201 tcaagtcacg gaatttaact gcataaccag atacccggat cgtcaagttc ggatatttca
     4261 ttggattttc cattgcatca cgcaataaat cattactgaa tacattgaca ttcaaatgat
     4321 aagctccttt tgagaagtat ccatctaaga cattacgcaa gttttcgatt cttgtatctt
     4381 gttcttttcc aagacctgaa ggattgattg tttgggtatt tgaaattcca tccaatgcac
     4441 agccgtaatt cattttagct gttgagttca ggctagctaa taacccactt tgttccgcat
     4501 tgtaacttgg atttgctcct ggcgcaagtg gttttccagc ttcacggcca tcaggagtat
     4561 ttcctgtatt tttaccgtaa acaacatttg aagtaatcgt caataatgaa gttgttggtt
     4621 ttgagttgcg gtaagtttta tgacgtttga tttgtcccat gaagtattcc aagatccatg
     4681 aaactaattc atctgcacgg tcatcatcat ttccgtattt agggaagtcg ccatccactt
     4741 caaaatcaac tgctattcct ttttcattac ggattgcttt tacttttgca tatttgatcg
     4801 ctgacaagct gtctgccgca tgagagatcc cagcaatacc tgtagcaaat gtgcgttgca
     4861 agtcagaatc catcaaagcc agctgagctg cttcataaga atatttatca tgcatataat
     4921 gaatgatgtt caacgtattg acatatagtt ctgctaacca atccatcata ttcatgaagt
     4981 tatgtttcac ttcttcaaag tctagtattt ctgatgtgat tggacgatat tctggtccaa
     5041 cttgggcgcc agtcacttca tcgactccgc cattgatggc atataatagt gttttagcca
     5101 agttagcacg agcgccaaag aattgcatgt cttttccgac aactgttcct gatacacagc
     5161 aagcaatcgc acaatcatca gaaccccatt gttcacgaag ctgattatca ttttcgaatt
     5221 gaatcgaaga actttcgata gcgattctgc tagcatatgt tctgaatcct tctggaaggt
     5281 gttcagaata caaaacagtc aagtttggtt ctggtgaagg tcccatattt gttaatgtat
     5341 gaagcagacg gaaatcattc tttgtaacta atgaacgacc atctaatccc atacctgcaa
     5401 tggataaagt agcccagata gggaatcctg agaataattg gttatattca ggtgtacgcg
     5461 cgaatttgac catacgtaat ttcattacta agtggtcgat caattcttgt gcttcttttt
     5521 ctgtgattac tccattatcc aaatcacgtt ggatatagat atcaaggaat gctgagatac
     5581 gtccgattga catcgcagca ccattttgtg atttgattgc tgctaaatat ccgaagtata
     5641 gccattggac agcttctttt gcatttgcag ctggtttaga aatatcatag ccatatttcg
     5701 cagccatttc tttcatttgc gctaatgcac gatattgttc agtgatttct tcacgtaaac
     5761 gaatcacttc ttcactcatt gtatgataac cagtcatatc atgatctttt ttcttttgtt
     5821 ccatcaaata gtcgattcca tacaatgcca cacgacgata gtcaccgatg atacgtccgc
     5881 gaccgtaagc gtctggaagc cctgtaatga ttttgttttt acgagcagca cgcatttcag
     5941 cagtataagc gtcaaataca ccttgattat gtgttttgcg ccagtcagtg aaaatatgtg
     6001 tcaattcatc gtttggtgta tagccattgc tttttagcgc attgttagcc atattgattc
     6061 cgccgaacgg catgaatgct tgtttcaaag gtttgtctgt ctgtagaccg acgatttttt
     6121 ccaaatcttt atccaaatag cctgcatcgt gtgaagtaat agttgatacg atttctgtgt
     6181 ccatatcata gacaccgtta cgctcgaatt ggatatcatt taacttttgc aattcatccc
     6241 ataatttttc tgttgcttct gttgggcctt ccaaaaagct gtcatcaccg cgatattcag
     6301 tgtagttatt ttgaataaag tctctagtgt ctacgccttc tttccatttg gtacctttaa
     6361 atccattcca ttgctccatt gtatggacct cctaaatttc tgttttatgt aacagttttt
     6421 acgtgattag tataacacag caccctatct ttgcaagggt tttcttgttt tttatttaac
     6481 aatttttatg ttttatttac atatatttaa atcaacgttg acataccaac atttaagcat
     6541 tgtgaacact ttcatctttt tttgcaaacg tataaggatt tattaaaaat gaatgaaaaa
     6601 gagacgccac ctgtattaat acagatggcg tctcttcatt aaaatactac aacagttacg
     6661 actcgatgaa aatctcagga gcatcgataa cttcgatgac ttctccacct tttttctcat
     6721 ccatcgcaaa tgatccattc gatgttcgat cacctatagg gaaatcagtt gggtcaatcg
     6781 ttaactgctg ccctttttga ttcatcaagg ttatttcttt tgatcttcct tccgacatat
     6841 agataatacg gtgcggattt tttttcagct cacgcatgac cgtcactccc cgcttcgcac
     6901 gtgaagtttg gctgatttct tgagcaagca tgcgtttaac agctcctcgc tgagtaacga
     6961 ttacgatcgg cgtatcacct tctgctaaca caagcagccc gttgataatc ctatctccat
     7021 ctttcaaatt catagacttc actccagctg cctttgctcc aacaacggga acatctgtta
     7081 aaggatagcg caaacccatt ccttgatgcg ttactaagaa aacatctaag cctggctgat
     7141 cttctttcag atacacagca atcagttcat cttcttccgc ctttagcttc atacagctga
     7201 gcggacggct cttataggtg cgccatggtt cgaactctgt cattcgtgtt tgcttgatca
     7261 atccattctt gctgatgaat acaaatgttt tctctgcttg aattttttga tatgggaaaa
     7321 cagctaaaat cgattcgtct acagacagat tggagattgt ttgagaaatg tgctcaccag
     7381 catctttcca tttcaaatcg ggtagttcat ggacagggcg gtagatcaca tttgctttat
     7441 ttgtgatcag aagaacatgg tccaatgtac tcaattcacc tgagtatagc aagtagtcgc
     7501 cttctcgcat cccgatttct tctggtttgg aagcactata agaacgaagg cttgttcgct
     7561 tcacatagcc ttctcgcgtc acactgacaa ttacgtcttc ttgtgccact aagacttgcg
     7621 tatcaatttt gatttcttcg atttcgtctt cgattgtcgt tagacgagcg gaagaataat
     7681 tttttttcac ttctcgcaat tcttttttca taacagagaa aagctctttg tcatttgaaa
     7741 ggattttatt caactctgtg atttgcgcaa tcaagctttc ggattccttt tgaagctctg
     7801 tgatatccgt gttcgtcaaa cggtataatt gcaatgtgac aattgcttct gcttgttctt
     7861 ctgtaaattg aaagacgtcg actaaattct ttttggcgtc ttttttgtct ttactttcac
     7921 gaatcgttgc gattacttca tccagaatcg ataaagcttt catcaatcct tctacgatat
     7981 gctgtctttt ttgagctttg gctaaatcga attgactacg ttttgttatg acttgctttc
     8041 gatgctcgat ataacttctt aaaatgtctt tcagtccaac ttgatgcgga gtcatatggt
     8101 cgatagcgac catattgaaa ttgtaattga tctgtagctc agtgtttttg aacaaataat
     8161 tcaaaattcc ttgagcattg gcgtcttttt tcaattcgac gacaatttgc agacctgttc
     8221 tatcactttc atctcgtact tcagctattc catcgatttt cttattcaaa cggatctcat
     8281 ccattttttt caccaaggtc gctttattta cttcatatgg gatttctgtg atcacgattt
     8341 gctgtttatt acccttcatt gcctctatct ttgttttcga acgaagaata acttttcctc
     8401 gtcctgtttc ataagctttt ttgatttcat cttttccttg caagatccct cctgttggaa
     8461 agtcaggtcc aggaatatat tccatcaatt tttcaaggct cgcttgtgga tgatcgatca
     8521 tatagattgt gccatcgatc acttcagcta agttatgagt cgggatttct gttgcatatc
     8581 cagcagatat acctgtcgat ccattgacga gaaggtttgg gtatcgagct ggaaggactg
     8641 ttggttcttt ttctgtatca tcaaaattcc acacgagatc gactgtttct ttctcgatat
     8701 ctgctagcat ctctccactt aacttggata atctggcttc agtataacgc atcgcagcag
     8761 gtggatcacc gtccatactc ccgttattcc cgtgcatttc gatcagtact tcccgcaact
     8821 tccagtcttg gctcatgcgg accatcgctt cataaatact gatgtcacca tgaggatgat
     8881 aattccccat gatatttccg acagattttg ctgatttacg gaatcctttg tcatacgtat
     8941 tgccatcttt gttcatggca aataatatcc ggcgttgcac tggcttcaat ccatcacgga
     9001 tatcaggcag tgcacgttct tggataatat atttggaata acgtccgaag cggtcaccca
     9061 tcacttcttc taaagttaat tcttgtactt cttgtcgatt ttccatcagg tgacctccta
     9121 ttctacttca aataactgat tgttttcatt tttatctgct cgttcttcat ctaataaatc
     9181 attagaaact gaaggagaga tttcagtatc ctctttttta tctaagatac tgccatcttc
     9241 ttcgagcgta aattgcacgt gattttcgat ccattttctt cgaggttcta ctttgtcacc
     9301 catcagtgtg gttactcgac gttcagcttg cgcagcatca tcgatacgga cgcggatcaa
     9361 cgtacgggaa gtcggatcca tcgttgtttc ccacaattgt tccgcattca tctctccaag
     9421 tcctttatat cgctgcagca tatatccttt gccgactttt ttaatcattg ctgccaattc
     9481 gtcatctgtc cacgcatatt caatgacttg cttctttcct tgtcctttgg atactttata
     9541 taaaggcggt aacgcgatat atactttccc agcttcaatc agcggtttca tataacgata
     9601 aaagaaagta agcagtaata cttgaatatg ggcgccgtct gtatccgcat cggtcatgat
     9661 gattactttg tcgtaattac agtcttcgat agaaaactca ggaccgactc ctgcaccaat
     9721 cgtatagatc atcgtattga tttcttcatt tttcaagata tcttgcattt tagctttttc
     9781 ggtattgatg acttttcctc gtaaaggtag aattgcttgg aactttctgt ctcttccttg
     9841 tttagcagat ccgccagcag aatccccttc gactaaatac aattcatttt tcttagggtt
     9901 tcgggattgt gcgggtgtca attttcctga aagaagagat tcgccttttt tacgcttctt
     9961 cccatttcgg ctttcttctc tggctttacg tgctgcttct ctcgcttctc tggctttgat
    10021 tgctttacgg accaacatct ggctcatttc gctattttcc tgcaaataaa agcccatttg
    10081 ttcactgatg acgttatcta ctacggtacg agcaactggc gtaccaagtt tttcttttgt
    10141 ttgcccttca aattgcagaa gattttcagg tacacggata gaaagtacag cagctaaccc
    10201 ttctctgaaa tcactacctt ccagattctt gtcccgttct ttcagcaaac caacttttct
    10261 cgcatattca ttataagatt tcgtcatcgc cgctttcata ccagcttcat gggttccccc
    10321 atcttttgtc cgaacgttat tgacaaaaga taaaacattt tctgaataac cgtcattgta
    10381 ttgataagcg acctctactt cgataccttc tttttctcct gaaaaataaa caactggtgt
    10441 caatgtatct ttttcttcat tcagataatc aacaaattct ttgattcctt cttcgtaatg
    10501 aaaaatttcc ttgactggtt cttctccacg aagatcagaa agagagattt taacgccttt
    10561 tagaagaaag gcagattctc ttaaccgttc ggctaaaatt tcatatgaaa atttcgttgt
    10621 actgaatatt gtatcatctg gtaaaaaatg gacagtcgtc ccgtttgctt tttttgtttt
    10681 tcctatcttt ttcaaagtac cgtcaggttt accgccgttt ttaaatttct gctgatactc
    10741 tacgccatct ctaacgatcg ttacaattag ccatttcgat aaggcattta ccacactggc
    10801 tcctacaccg tgcagtccac cagaagtttt gtaccctcct tgaccaaact ttccgccagc
    10861 atgcagcaca gtaaaaatca cttcaactgt tggtattccg gaagcatgca ttcctacagg
    10921 catcccacgt ccgctatctg caacagttat actattgtct tcatggatgg tcacatcaat
    10981 ctcactgcca taacctgata atgcttcgtc aactgcatta tcgacgattt catagactaa
    11041 atgatgcaag cctcggctgt cagtggatcc aatgtacatc cctggtcttt ttctaactgc
    11101 ctctaatcct tccaatactt ggatggacga gtcattgtat tcgttgttta ctttttttgc
    11161 caaggcaaac gctccttcaa taatactttg cggcaaaacc gcaccacgat tatcatacta
    11221 gaaaaggtcg tgaaaaaaaa gagggtagac gagaaatcaa ttttaattat ctcctaaccg
    11281 ctttttcgac attcccctct atcgatcatc tcttctttgt ggtcattttt atttttggtt
    11341 ttctttcaac ttaccaagtt caatcttaat acaacgatcc attacgatat ctgtacgtcc
    11401 tgcttttctt agcatctcag ctgcttcttt gttttcaatc cctaattgtg cccagaacac
    11461 tttagcatca gttttcaaaa aatcacctgc tatttcaggt aaaaattcac tccgtctaaa
    11521 tacatctacg atatcgattg gatcatcgat ttctgtcaaa ttcgcataga ctttctcgcc
    11581 tagaatttct tcaccagcca ataatggatt tactggtatg attttatatc cattgtcttg
    11641 caatacctgt gcaaccatgt aacttgttcg ttctggttta ttgctcaagc ctacgacggc
    11701 aatatttttt gcttcttcca aatagttgaa aatcgtttct tgtgttggat tttttacaga
    11761 catttctgcc accccttgct tttcttttta ttccatctat tatacttgta tctttcattt
    11821 tttcaattgt tcctgttacc tattcatgct aaaataaagg atagttttca acttgaaaga
    11881 gggtaaaaaa tgaaattgat cttattactt attttagcat atcttttagg ttctattcct
    11941 tccggtgtct ggattggcaa attgtttttc aaaaaagata tccgacaaca cgggagcggt
    12001 aatactggaa cgaccaatac atttcgtgta ttaggcaaaa cagccgggat tgttgtcctg
    12061 cttatggata ttttaaaagg tacattggct acttgtttgc cgatgatctt ccacctttct
    12121 gggattaacc ctctttggtt tggggtatgc gccattcttg gccatacatt tccgattttt
    12181 gctgggttca aaggaggaaa agctgttgcg acaagtgcag gaatgctttt aggatttaat
    12241 cctgtatttt ttgtttattc ttgtatcatc tttatcgtta gtttattttg tacaagtatg
    12301 gtgagtttga gcagtatgat cagtgcagta ttgatcactt tatctacgat cgtccttcct
    12361 tatgtagctc ctgtgatttt ggctaagccc aattggttac tgacgatcat tgccttttta
    12421 gtttcttcct ttatctttta tcggcataaa gacaatatca agcgtatcag aaaccatact
    12481 gaaagtcgaa tcccgtttgg tttgaatagc agcaagaaaa aatcctgata ctttttcatt
    12541 ccaaagagat aaaacgggag tgtgacaaat cgatgtcaca ctcccttatt acttacagga
    12601 tagctttttt acacaccttg gtaaacggtg ttccctgcta acttctcggt cacaacctct
    12661 tacttcactg tgatggaata ttttgtcttg aagatttcat gggcacctag ttgattgatc
    12721 cctacttttt ccatcaagtt tccactactg ttcaccgtgt cagcgattcc gcaccacggt
    12781 tcgatacaaa cgaaaggcgc ttcttttgga taaggagacc agatccctac ataaggcata
    12841 tctttatagc ttaaagttac gctatgggaa gatttttcac ttcgaatggc gtacgcattt
    12901 agaccccttg tttcaaaaac caaggcatct tgctcaaaaa gttcacgatt caaagccaga
    12961 ttcgtatttg tctgacctaa cgtcttttga tccatatcga tgaatgggcc tttcaatggt
    13021 aggcgtaatc ttgatttcaa tggagaaaat gccagataga aatcttcaaa tgacaatcct
    13081 tcttcaagag gtacattgaa agcaggatgt ccaccgatag aaaagaacat ttctttttca
    13141 ccggtatttg ccacttgata atgaaccgtg atgccgtctc ctcctaattc atagctgatc
    13201 actaattcaa aatcaaatgg atacttttct aatgtctccg gcgtactcct taagatgaaa
    13261 ctagccattt cttgtccatg ttcaatgact tcaaaatcca tatcccgagc aaatccgtgt
    13321 tgccccattt gatatgtttt cccttgataa acatactgat catcttttaa acgtccaaca
    13381 aaagggaata aaaccggtgc atgtctgccc caatacgctg gatcaccttg ccagatatac
    13441 tctaaaccat gttcttttga tttcagactg atcaattccg caccgtgttc agcgatttcg
    13501 gcgatcagct ggtcattttg aataataaca gtcattgcca ttcctccaac ttcgacttac
    13561 tttttaaaat gggaaggctg gaacaaacat ttctatctgc cccagtcccc ttacttctaa
    13621 taactgtgtt ttgctaactc gtctttaaaa cgggaattca atacttttaa ataagttccc
    13681 ttcatgccta acgaacgaga ttcaatgatt ccagctgatt ccaattttcg taaagcattg
    13741 acgatcacag aacgagtgat gccgatacta tcggcaatat tagaagcagt cagacgacct
    13801 tcatctccat ccaatgcttc aaagatagct tgtacagcct tcaattcgct ataagataat
    13861 gtgttgattg ccatttggac agccgttgca ctccgtacat ctgcttcgat gcttcgagac
    13921 ttctgataaa ggatctgcat accgacaacc gtagctccat attctgctaa gactagatcg
    13981 tcatctgcaa acgattgttc catacgagat aagataatcg tacccaaacg ttcgccagct
    14041 ccaaagatcg gaacgacagt cgtcagacca aatggatatt tctctcttga ctctaccgga
    14101 aatgcagtaa tatcactagt aattgggata ttagcttcag ttttcatcaa ctgtgccact
    14161 gtatcagtat aagattcagg aaattgtttc tcaacaaaca ttgttttgat tcgttcattg
    14221 ttcacatcgt gacgttcatt ataacctagt aatactccgt cacttcctat gatataggtg
    14281 ttgctgtcaa gaataccacc tagtgtgaca gccattttat cgtaaggcaa atctgcttgc
    14341 atgtcaaaag tattcttttg ctgcaaaagt gtgtttatct tgcgggtttt cgttagcaag
    14401 tctgtcattg tcgtcctccg attttttata aaatatagcg acttaaatct tcatttgccg
    14461 caattgagcc aagtttgtca tccacatatg cttctgtgat cgttatttct cccatttgca
    14521 tatctggtgc ttcaaataat agatcttcta gtagtttttc caaaatggta tgcagtctgc
    14581 gtgcgccgat attatctgtg tcacggttga catcgaaagc gatttgagcg atccgttcaa
    14641 ttgcttcttt tgtaaacgta actgacacat tttctgtacc gatcaacgcg acgtattgtt
    14701 ttactaaagc attatttggt tcggtcagaa tcttcacaaa atcttcagct gtcaaatcat
    14761 ctaattctac ccgtatcggg aagcgtcctt gaagttctgg aattagatca cttggtttag
    14821 acaaatggaa agctcctgaa gcaatgaata ggacgtgatc tgtttggatg atcccgtatt
    14881 ttgtgtttac ttgtgaacct tcgacaattg gcaatatgtc tctttggacg ccttctctgg
    14941 atacttctcc agattgctgt gatttagaag tgatcttatc gatttcatcg ataaaaataa
    15001 ttccactttc ttccgctaat ttgattgcct cactatggat atcagcatcc ttgactaatt
    15061 ttgctgattc ttccttgatc aataactcta aagcttcttt gactgttacc gtccgttcca
    15121 cttttttctt cggtgtcaaa gcacctagag tttcatttag gtcgatcccc atttgctcca
    15181 atccattgtt catcattggt gcaggtttct tttggtcttc aacttcaatg gttacttcac
    15241 gactatccag taaacccttt tgtagttgtt ccaagactgt acggcgattg attcgaatat
    15301 cttctgtcac ttcttcttgc ggttcagcgg tattttgctg actagcatta aaaagattca
    15361 tcatttgttc aaattgatta gtggattgtt tctgttcttt tttgatacca ggtaccaaga
    15421 tttttaccaa acgtttttct gcattttttt tagcacttgt gtatacacgg ctgtactgct
    15481 ctttttctac gatttggatc gcattttcta ctaaatcccg aaccattgat tctacatctc
    15541 ttccgacata accgacttca gtaaattttg ttgcttcgac tttgacaaat ggtgcattga
    15601 cgatttttgc caaacgtcgt gcgatttctg ttttaccgac ccctgtaggt ccgatcatca
    15661 gcatattttt tggtgtaata tcttgttgca ttttttcatc taactgcaaa cggcgatagc
    15721 ggttacgcaa agcaacggca acagattttt ttgctgcgtg ctgtcctacg atatattcat
    15781 ctaattcact tacgatttgt cttggtgttt gttttaattc atacatatct actcacctct
    15841 tacagttctt ctacgataat attatgatta gtaaagatac agatatctgc ggcaatattt
    15901 aacgcatttt ctgcaatttc tttcgcactc atgtcgtttt gagcatagtt tttcattgct
    15961 cgggctgcag ccaacgcaaa attccctcct gatccgatag ctaagatacc atcatctggt
    16021 gtgataactt ctcctgtacc tgaaacaaga agcatttctt tatcattcat tacgatcagc
    16081 atcgcttcaa gtttttgcat agattgctgt gtccgccatt cttgagctaa ttcgacagct
    16141 gcgcgttgta gattcccgtt atattcgttt agtttgcctt caaatttttc ttctaaagta
    16201 aacgcatctg caacacttcc ggcgaaacct acaacaactt ctccattgta gatacgtcgt
    16261 actttctttg cagtgccttt catgacaaca gattcaccca ttgttacttg gccatctcca
    16321 gccatagcaa atttcccgtc tttttcgacg gcacagatag tggttgaatg aaatggtgat
    16381 tcgaccatgt ttgtttcctc ctaaatttgt tcttttcctt atttttacgc tcttggatgg
    16441 aaagaacgat agtttttttg taaactttct tttgttacat gggcatagat ctgggtagtc
    16501 gataaattgg catgtcctag caattcttgg actgtccgca aatctgcccc attatttaat
    16561 aaatgagtag caaatgtatg gcgcagcatg tgaggatgga tatcactatc caaactgctt
    16621 tttttgatca tttgattcaa gacatattca attccagtag gcgtgattgg ttttccatga
    16681 tgattcacaa aaacgatatc atgctcttcg tgatattttg tcatcaatac ctggcggcct
    16741 tccctgaaat attcttgcag agcatcttga gcaaaagaac ctagaggagc atatctctct
    16801 ttgttccctt taccatggat caacatgacc tctgaatcaa aatcaatgtc tgataccgac
    16861 aaactgctac actcacttaa acggatgcct gacccgtaaa ggacttccag taaagcgcgg
    16921 ttcctcaaat ccaaaggact atctcctgct acgctgtcaa aaagtgcttg catttcattt
    16981 tcataaaaaa aacgtggcag tctcaaattt ttctttttga gatgaacgta agaaaatgga
    17041 ttttctttta tcacttcttc tttgagaaga tactgataaa acgaacgtag gctggctatc
    17101 ttgcgactga tagaatttct gctatagcta ggacgatgat cagctaatgc actcaaatag
    17161 acacgaaccg taaaatattc cgccttcagc agatcagcgt ctccagattc ctccaaaaaa
    17221 tgtttgaaat ggagaatgtc ttcttcatat gcttctttcg ttttttctga atatcctctt
    17281 tcgacaatca aataacgcaa aaatttttgt tcccacgtgg cttctgtcat tttctcacct
    17341 tccttttgtc ccagagaata atttagcaca gcgtttataa aaagacaaat aaattgtcag
    17401 aatttaacca atatttctaa gtttgttcac aattggcgca attcctcaca ttttgtttga
    17461 gaaattctta agaaaaacgc aagaaaaaga gcgagactat ccgtctcacc ctcttcatgg
    17521 agtttcctta ctcatttggg attgcagttt tttctaattc tgtttttatt gcctcattag
    17581 cttttaaagc tcgttgtgca attgcttcat atcttgcttt tttgtctcga atgcgctctg
    17641 gcagttcagg aaacagacca tagttggcat tcatcggttg gaaatgtttt ccttccgcat
    17701 gagtaatgta gtaagccata cttccaatag ctgtttcttg tggaaactcg actggttcca
    17761 tttcttttgc taagcgtgcg gcgttgattc cagcaagtag tccgctcgct gcactttcaa
    17821 catagccttc tacaccagtc atctgacctg caaagaacag atcttcgcgt ttcgttgatt
    17881 ggtaagtcgg tttcagcaat tctggcgaat tcataaagct gtttcggtgc atcacaccat
    17941 agcgaacgaa ttcagcattt tctaacccag gaatcatccg gaaaacacgt ttttgctcgc
    18001 cccatttcaa gtgagtctga aagcccacga tgttgtataa agaagctgca gcattgtctt
    18061 gacgcagctg gataacagca taaggacgtt tgccagtttt aggatcttcc aatccaactg
    18121 gtttcatagg tccgaaaagc atcgttttga atcctcgctg tgccattact tcaattggca
    18181 tacatccttc aaaatatttt tctttctcaa aagatttaag ttctgccact tcggcattga
    18241 ttagtgcctc atggaaagct ttgaattcct cttctgtcat cggacagttc aagtaagctg
    18301 cttctccttt gtcatagcgg gacttcaaat atactttatc catatcgatc gtagctttgt
    18361 ctacgattgg tgcagctgca tcatagaaat aaaaaccatc tgaaccattg aatgctttga
    18421 tgctttctgc taatggatga gaagtcaaag gtcctgttgc tactacggtg attccttccg
    18481 gtatttcagt aatttcttcg tttatcactg ttactaatgg atgctctttg acttttcggg
    18541 taattgtttc agaaaaagaa tcgcggtcaa cagctaaggc accaccagct ggcactgctg
    18601 tttcatctgc cgaagtgatt actaccgaat tcaatcggcg catttcttct ttcaataacc
    18661 ctactgcatt agccaaactg tttcctctta atgaattgga acatactagt tcagcaaaat
    18721 tacttgtgtg atgcgcctca gtcgatttga ctggacgcat ttcatataaa cggacaggga
    18781 ctcctgcttg tgcgatctgc caagcagctt cacttccggc aagccctgct ccaacaacat
    18841 ttaccatttt caatgaaaga acctcttttc tattattttt ggatgtcttc ttcgtaatct
    18901 ccatttacac agactacttg tttaccgcct ttgactttct tctcgaccag atatccgtca
    18961 cattttggac aatttctgcc cactggttta tcccaagaag tgaattcaca ttctgggaaa
    19021 cgggaacaac catagaatag tctgtttttc ttagattttc gttcaatgac ttgtccttca
    19081 tgacatactg ggcaagtcac accgatttct tttacgattg gtttcgtatt ccgacaatca
    19141 ggaaaattac tgcaagcata aaacttccca tatttcccta attttatcac catcggatgt
    19201 ccacagacat cacaatcgaa tcctgctggt tcatctttga tttgaatttt ttcgattttt
    19261 tcttccgcat tcgtcagttc tttttcaaac ggtttataga aacgatcaac cactttgacc
    19321 cacgcttccg tgccttcttc aattttatcc aaatctcctt ccatttctgc tgtgaaatgg
    19381 atatcgacga tttgagggaa gaattcacag attaatgaat tgacaatttc tcctagttca
    19441 gtcggctcaa atcttttttg tgtcaatttt acatagtagc gtctttggat cgtatcaagt
    19501 gttggtgcat aagtggaagg tcgccctacc ccgttttctt ctagtgcacg aatcaacgta
    19561 gcctcactaa aacgtgctgg tggttgagta aaatgttgtt ttggttcaat atcaacagat
    19621 tggactacat cgccttcttc aagttctgga agaatatttt ctttctcttc tttgccgtca
    19681 tctctacctt ccacatacac ttgcatgaat cctttgaatt tgatttttga accgttcgca
    19741 atgaagatga caccattttg ttctaatgtc actttcattg tatctaaaat agctggtgtc
    19801 atctgactag ccacaaaacg tgaccagatc aatgtataaa gtttcaattg atctttatct
    19861 aaatattgtt tgagttcatc tggtgtacga agcacactcg ttggacgcac agcttcatgg
    19921 gcatcttgtg cgccttgagc atttttgatc tttcggtgat catgtctaga aaattctttt
    19981 ccatacgtat cttcaataaa tgctgcggcc tctgcttttg ctgtatcggc aatccgggta
    20041 gaatcagtac gcatgtatgt aatcaagccg actgttcctt gtttacccaa agtgattcct
    20101 tcatataatt gctgtgctac catcatggtt tttcttgttc gaaagtttaa cttacgggca
    20161 gcttcttgtt gcaggctact tgtagtgaaa ggattcgcag ggtttctttt gcgctctttc
    20221 ttttctactt ttttaacttc gaagtctgtt ccatcaattc tagcagtgac ttcttttaca
    20281 ctttgtgcat ctggtaattt ctttttctta ccgtcgattc cccagaaatt tgctttgaat
    20341 ttcttgcgag cttttttaaa attaccgtcg atgctccaat attcttctgg aacaaattct
    20401 cggatctctt tttcacggtc aataataatt tttaaagcga cagattggac acgtcctgca
    20461 cttaaccctt tcttgacctt acgccagaga ataggactga tcgaataacc aacgattctg
    20521 tctaaggtac gacgagcttg ctgtgcatct actaaatcta catcgatcgt tcttggttcc
    20581 ttaaaagctg ctttgactgc gtcttttgtg atttcattga agaccacccg atttttttct
    20641 ttcagatcca acccaagtag gtacgctaaa tgccaagcaa tcgcttctcc ttctctatcc
    20701 gggtcacttg ccaagtaaac tttttgagct ttttttgctg cggctttcag gcttttgatg
    20761 acatcgcctt ttccgcgtat agaaatataa tggggttcat aattgttttc aaaatcgata
    20821 cccattttac ttttcggtaa atctcttata tggccaacac tggccattac tttatagttt
    20881 cgtccaagat atttctcaat tgtcttggct tttgcaggtg attctacaat cactaaatat
    20941 ttatacgcca ttactgaatg gactcctttc cattgtcgtg ttcctactat taaaagagga
    21001 ttcatacaaa tcgtagctaa tcatatccat tcagaaaacg tttgtcaaga aaagcaaatg
    21061 aaaagtatgg tgttattctt taaattctgc caagatatct tcacccgaaa ccgtgcaaac
    21121 tgcaccgtct tggatcaaat gatgacatcc ggtagaatgt tctgttaaaa tatttcctgg
    21181 aacagaaaaa actgttcttc caagatccaa tgcttgctgt gctgttatca gagagccgct
    21241 ttttttcttg gcttcaaaga cacatgttcc tttagacaaa gctgcgataa tccgatttct
    21301 cattggaaaa caatgctttt tcgggccgga ttgaggagga tattcactca aaagaagatg
    21361 atcatttgca atcacctctt gcaagtcacg attttcagca ggatagactt gctctatccc
    21421 tgttccaatg actgcgattg tccgtccacc atgagagatt gctgcttggt gaccgtatgt
    21481 atctatccct ttcgcgcagc cactgacgat cacatatcca gcttctatca ctggttttag
    21541 taaataataa actgtttttt tggctaaagc agaagtgtgt cttccaccaa caacgcttaa
    21601 catgttacaa gaaagtaaag atagatcacc attataaaac agtacaattg gcggataggt
    21661 caagtgaagc agctgagggg gatatttggg atcaaagcaa gtgataaacg actgtttcga
    21721 ttggattttg ccccagttag cgtgaatttc ctgccaactt ttgcgaaaca gctcccggtg
    21781 tttggttatt ttacctatct taatgatgtc ttcaaatccg atttctttat aaggatactg
    21841 catcatccag ttagcaagtg cccattttcc ttgactactg attccttttg tataagcaag
    21901 tttcaataaa ttttcttcta tttgatacat cataaaaccg cctttcgtct ctctttctaa
    21961 taagatacgt aaaagcggct gttttttatt gtaaatattt ttttacaggt gcaaaacttt
    22021 tacgatggat tggcgtaatt ccttgtcgtt caagtccttc taagtgttcc tttgttccat
    22081 aacctgcatt atgcgagaaa ccatatccag gataaagcac gtcatattct ttcatcaatt
    22141 ggtctcgata tactttagca atgatacttg cagcagcaat cgacacagat cgtgcatccc
    22201 ctttgattat tttttcctgc tgaataggca aatccaaggt catcgcatcg atcaacaaat
    22261 agcttggtac aatggacaat ttttctactg cttggatcat cgctaattta cttgcttcat
    22321 aaatattcac atcatcgatt gtctgctgat caacgatccc gatcccgatt gccagggctt
    22381 tttcttgaac ttgttgataa agaagttctc gctttttctc tgataattgt ttggagtcat
    22441 tcagccctaa tatgatctca tttttcggca agataactgc agctgccacg acaggtccag
    22501 ctaatggtcc tcttcctact tcatcaatgc cggcaatcca ttggtgacct tgtgcgtacc
    22561 cttgcttttc aaagcaaaac atttcttcat atttttcctt aattaatgct tcctttttca
    22621 atcgtcgttc aaaagaagcc agtgcttgct gcacgccttt gcgtggatca tttcttaatg
    22681 attctagtgt aggatcatct attctctgta tgcttttcaa tcgttctttg atactttgga
    22741 tggattcagt catcactttt ttgctccatt tcttcgcaac gatccagtgt gtatcgtcca
    22801 agttttcctt gacgaatatc atgcacgact aattcgctgc cacgatcata gtcttcttta
    22861 tacccacgtt ttgctgtaat atccatcagt aattccggtg ccaatttttc ttcttgctcg
    22921 attgataaac cataacgatc gctgattctt cctgaataat atcgggcaaa aaagtctaat
    22981 ccgtaaatcg ctaaatcatc taaatggaca agctggtctt tgatcgcgcc tgtcagcgct
    23041 agttttttgc cgatttcttc atcttcaaat ttgggccata aaatccctgg tgtgtctaac
    23101 aattccaact cattacctaa acgaagccac tgctgacctt ttgttacgcc aggcttattt
    23161 cctgtctgtg caattttctt gcctactagt cgattcatca acgtagattt cccaacattt
    23221 ggaataccta agaccattgc ccgaatagcg cgaggcttga tcccccgagc agcatctctt
    23281 gcccgttttt ctttcaatgc ttctttagcc gcaggtacta gacctttaat tcctttactt
    23341 tcttgtgccg tgatagctaa tgcaagatgg ccttgtttac gaaaatattc ttgccacagc
    23401 tttgtctgtt gtggatctgc cagatccgct ttattcaata aaacaaggcg aggtttttgc
    23461 tgaatgatct gatctagcat tggattacga gaagataaag gtaatcgtgc gtctaccaat
    23521 tcaaatacga tgtccacata tttcattttt tcagaaactt ctcgtcgggc ttttgccata
    23581 tgtcccggaa accattggat ggtcattgtt ctctccattc ctttcatacc ggaatattcc
    23641 ccggctttac ttgttttttt ctttttcaat ataaattcac ttttttatta taaagaaatt
    23701 ccatctatta gttaagtgaa agtgaaccag tcctcattct atcacatctt cacttttttg
    23761 atgatgcaaa tttttatttt ggtttctata tatgaatcta aatttttgac agcgtttcct
    23821 aaataagaga tgatagacga acaaaacaga aaggagacga aacaaatgaa tagtatggtt
    23881 tttgttaatt ttcctgttgc tgatgtcaat cgttccgttg ctttttatga aaagctgggt
    23941 tttaaaaaaa atgaagaatt ttctactgaa gaagctggtt caatggtatg ggatgacact
    24001 ttttggataa tgcttctctc tcatgatttt tataaaaaat ttataaaaga taaagaaata
    24061 gctgataata aaaaaacaag tggtgctttg atttcgttta gtgttgaaaa tgcggatgaa
    24121 gtaaaaaaat ttgctcaaat tgcaaaagaa aatggtggta ctttccacga agtagatatg
    24181 ggaatgcctg aagaagaaat gttttcatta gaagtccaag atctagacgg aaatacatta
    24241 gaacccgtat ggatgaagat gtaagcagat aactaatctg acgtttttta taacaaaaaa
//
LOCUS       DAGCWU010000024        23431 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.024, whole genome shotgun sequence.
ACCESSION   DAGCWU010000024 DAGCWU010000000
VERSION     DAGCWU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.024"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(6..3680)
                     /gene="rpoC"
                     /locus_tag="MBZ71_000793"
     CDS             complement(6..3680)
                     /gene="rpoC"
                     /locus_tag="MBZ71_000793"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBT4428765.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3738..7355)
                     /gene="rpoB"
                     /locus_tag="MBZ71_000794"
     CDS             complement(3738..7355)
                     /gene="rpoB"
                     /locus_tag="MBZ71_000794"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBT4428766.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8083..9051
                     /locus_tag="MBZ71_000795"
     CDS             8083..9051
                     /locus_tag="MBZ71_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4428767.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9069..9974
                     /locus_tag="MBZ71_000796"
     CDS             9069..9974
                     /locus_tag="MBZ71_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428768.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9971..10777
                     /locus_tag="MBZ71_000797"
     CDS             9971..10777
                     /locus_tag="MBZ71_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4428769.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10913..11902
                     /locus_tag="MBZ71_000798"
     CDS             10913..11902
                     /locus_tag="MBZ71_000798"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBT4428770.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12219..12743)
                     /locus_tag="MBZ71_000799"
     CDS             complement(12219..12743)
                     /locus_tag="MBZ71_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBT4428771.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13030..14727
                     /gene="scm"
                     /locus_tag="MBZ71_000800"
     CDS             13030..14727
                     /gene="scm"
                     /locus_tag="MBZ71_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBT4428772.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(14882..15868)
                     /locus_tag="MBZ71_000801"
     CDS             complement(14882..15868)
                     /locus_tag="MBZ71_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBT4428773.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(15891..16457)
                     /locus_tag="MBZ71_000802"
     CDS             complement(15891..16457)
                     /locus_tag="MBZ71_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBT4428774.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16471..16887)
                     /locus_tag="MBZ71_000803"
     CDS             complement(16471..16887)
                     /locus_tag="MBZ71_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBT4428775.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17344..19278)
                     /locus_tag="MBZ71_000804"
     CDS             complement(17344..19278)
                     /locus_tag="MBZ71_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4428776.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19559..20515
                     /locus_tag="MBZ71_000805"
     CDS             19559..20515
                     /locus_tag="MBZ71_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBT4428777.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20605..21708
                     /locus_tag="MBZ71_000806"
     CDS             20605..21708
                     /locus_tag="MBZ71_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBT4428778.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21721..22674
                     /gene="menA"
                     /locus_tag="MBZ71_000807"
     CDS             21721..22674
                     /gene="menA"
                     /locus_tag="MBZ71_000807"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBT4428779.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT         6893 a         4930 c         4557 g         7051 t
ORIGIN      
        1 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
       61 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa
      121 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
      181 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
      241 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
      301 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
      361 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
      421 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
      481 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
      541 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
      601 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
      661 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
      721 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
      781 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
      841 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
      901 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
      961 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
     1021 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
     1081 aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct
     1141 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
     1201 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
     1261 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
     1321 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
     1381 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
     1441 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
     1501 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
     1561 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
     1621 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
     1681 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
     1741 agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
     1801 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
     1861 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
     1921 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
     1981 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
     2041 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
     2101 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
     2161 tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc
     2221 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
     2281 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
     2341 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
     2401 cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
     2461 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
     2521 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
     2581 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
     2641 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
     2701 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
     2761 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
     2821 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
     2881 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
     2941 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
     3001 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
     3061 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
     3121 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
     3181 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
     3241 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
     3301 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
     3361 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
     3421 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
     3481 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc gcgttcaggt
     3541 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
     3601 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
     3661 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
     3721 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
     3781 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
     3841 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
     3901 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
     3961 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
     4021 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
     4081 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
     4141 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
     4201 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
     4261 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
     4321 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
     4381 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
     4441 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
     4501 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
     4561 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
     4621 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
     4681 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
     4741 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
     4801 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
     4861 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
     4921 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
     4981 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
     5041 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
     5101 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
     5161 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
     5221 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
     5281 gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca
     5341 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
     5401 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
     5461 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
     5521 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
     5581 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
     5641 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
     5701 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
     5761 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
     5821 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
     5881 gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
     5941 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
     6001 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
     6061 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
     6121 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
     6181 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
     6241 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
     6301 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
     6361 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
     6421 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
     6481 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
     6541 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
     6601 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
     6661 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
     6721 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
     6781 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
     6841 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
     6901 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
     6961 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
     7021 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
     7081 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
     7141 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
     7201 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
     7261 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
     7321 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
     7381 tcaaaaagac tacccgatca attgttacaa taatatttcg aatattattt ttaggtaaca
     7441 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
     7501 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
     7561 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
     7621 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
     7681 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
     7741 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
     7801 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
     7861 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
     7921 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
     7981 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
     8041 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
     8101 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
     8161 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
     8221 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
     8281 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
     8341 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
     8401 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
     8461 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
     8521 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
     8581 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
     8641 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
     8701 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
     8761 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
     8821 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
     8881 gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag
     8941 aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
     9001 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
     9061 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
     9121 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
     9181 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
     9241 atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt
     9301 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
     9361 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
     9421 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
     9481 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
     9541 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
     9601 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
     9661 ctggctcctt acttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
     9721 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
     9781 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
     9841 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
     9901 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
     9961 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    10021 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    10081 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    10141 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    10201 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    10261 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    10321 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    10381 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    10441 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    10501 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    10561 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    10621 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    10681 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    10741 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    10801 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    10861 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    10921 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    10981 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    11041 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    11101 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    11161 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact
    11221 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    11281 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    11341 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    11401 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    11461 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    11521 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    11581 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    11641 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    11701 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    11761 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    11821 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    11881 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    11941 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    12001 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    12061 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    12121 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    12181 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    12241 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    12301 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    12361 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    12421 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    12481 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    12541 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    12601 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    12661 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    12721 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    12781 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    12841 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    12901 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    12961 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    13021 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    13081 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    13141 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    13201 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    13261 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    13321 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    13381 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    13441 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    13501 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    13561 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    13621 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    13681 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
    13741 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
    13801 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
    13861 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
    13921 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
    13981 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
    14041 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atcaacttca
    14101 agcgacagta cagatactac ctctaccaca agtgatagca cggatacatc agcttcaagc
    14161 gacagtacag atactacctc taccacaagt gatagcacgg acacatcagc ttcaagtgac
    14221 agtacagata ctacctctac cacaagtgat agcacggaca cgtcagcttc aagcgacagt
    14281 acggatacat ctaccacaac aagtgatagc acggacacgt cagcttcaag tgacagtaca
    14341 gatactacct ctaccacaag tgatagctca gattcatcta ccactacaag tgatagcacg
    14401 gacacgtcag cttcaagtga cagtacagat acatcctcta ccacaagtga tagctcagat
    14461 tcatcaactt caagcgatag tacagacacg tcgtctacca caagcgatac acgtcatgca
    14521 agtgtctcct tctcaaaaac aagttcggat aacaggacga actttaactt aactaataaa
    14581 acaagcaatg acaatggaaa caaatcaaaa agagcacttc ctaaaactgg aagtcaatcg
    14641 aataattgga taacactcgc aggcgttata ttacttgtaa tcggtttaag aatcactttt
    14701 atgagttata agaaatcgaa aaggtaaaaa aatattcaag ataaaaaaga gcaacgtcgc
    14761 taacggacgt tgctctttat ttttgctttt tatcataaat acaagaacta caattcaatt
    14821 tgtcaaaaaa cacgtagatt tccttctgag aaatttattt acgtatcgaa cctttcccta
    14881 ttcagtcttt gcgatccaaa atcagttggg taagggaaaa gagattctct tttgttcgaa
    14941 gagaggtggc aggtaatttc tttatttcct tcaaagcctt ttctgtgtaa gaggccgcta
    15001 acttcctagc ttcttccacg cctcctgtat gttcaacgat tttgcgtacg gcgtcgacat
    15061 cttcttctgt cattgccgct ttcttctcta aataaggtag tagctttggt ttgttttttt
    15121 gcaaagagta gatcaacggt aaagaataga ccccttgacg catgtcttct agtacaggtt
    15181 tgccgatttc ctggctctct tttgtataat ctaaaatatc atcgataatc tgaaaggcca
    15241 tcccgataga aagtccgata tctcctgctt tttttgcgaa gcgctcgctt gttccacttt
    15301 caaaagcgcc aacagaacaa cttaattgga acagttcagc tgtcttacct gaaatatttt
    15361 ccaaatattc ttttacagaa agttcataat tatagcgatc atccatctgc cctaattcgc
    15421 cattcaatac tttttccata ctgcgagaat tcaattggag acttttcaag gaagtggagt
    15481 aatcagacaa tagtttaaaa cagcagacaa ataaatagtc tcctgcatag acagaaacga
    15541 cattaccaaa ctgactacgg atattaggct gaccacgtcg cagatccgct tcatcaacga
    15601 tatcatcatg gatcagtgtg gccgtatgga gcatttcgat agaagctgcc aaagcgatgg
    15661 ctttgtctcg atcttgctct ggtccaaatt gtgagaacaa tagctgatag gcaggacgca
    15721 acagctttcc gccggcatgg atcgtttctt tgatggcttg ttcgacaggt ttgtttttca
    15781 ggcggacagt ttcttccatc cgttttagtg tttctgtcaa ttctttactt aaagcaggat
    15841 agttttccca cattggatgt agcttcataa aaaaactcct tgtgtgacga ttattttttg
    15901 gtaaacattg ggctccattt ttgtttggtt ccctgctcat aatctttttg gaatcgttga
    15961 agtgtctgca catgtcgtaa aagataattc gctgcgatcc cgattgcaat ccctgataaa
    16021 atccctaaaa ctgaaaggac tggcaagtac aacatgactg tccatgattg tgcaatgaaa
    16081 gaagccatca aaagctgtcc gacattatgc atgaaaccgc cggccgcact aattccgatg
    16141 atactgacac gttttggtcc taattgcttg accaataaca ttccaaaata actaagcaaa
    16201 gcaccactca tactataaag gaatgtggaa atcgttccgc ctagcaaagt agtcaggaat
    16261 agtcgtagcc aaagtagtaa gaagctgtct tgttttttca tggtaaaaat ggcaacgatc
    16321 gtgattagat tggctaagcc tagttttgca ccaggcgcaa aagaaaaagg ataagggatc
    16381 atattttcca ataaaccaat gatcactcct tgtgcaacaa gtagcgaaat ataaatcatt
    16441 ttttgtaatt tactcatggt gtgtgaaggt ctaataaatc agactgtctt cgtcacctcc
    16501 atctgttgaa cggacttcga tcactaattt atgtggcaaa cagacgatgg tttcgccatt
    16561 tttcgttgcc cagcctcgac gtacgcagat ctggtcatcg caatctgctt cttttatccg
    16621 tactcgatcg ccatctatct cgatcaagtt ataatctccg tgttcatctt tgtaagtata
    16681 tgtgttgctc tcttctccag ccatcaaagg gaatgttttg atttctgtgc catccacacg
    16741 cagtaccgct tctttttctg ctgttgcggt gttttgctga agaccaaaaa tcatgactgg
    16801 taaaaaagaa agaacagtca aaagcaaaat aataaaaaca tcccaaggtt tgagcagact
    16861 tttttcagca aattctttaa ggctcatttt tgttctcctt tttccagatt aactcaccca
    16921 attttagcat aaaaacaaga ggttgtgaca cctgtgtctt gctttgagta ataagaacga
    16981 agaatagaaa acagctttcc atgttttcta attctttggg cttattaccg agaagcagca
    17041 ggtggaacac cgtttataac aagaggttgt gacagaagtg ggcaacttca agaaataaga
    17101 aggaatttac gaaaattact tttcaagttt ttgtgaaatt tcggcttatt tttcgaagaa
    17161 gctacttctg gaacaccgtt tataacaaga ggttgtaaca gaggttgtga cacctgtgtc
    17221 ttgctcatgg aggcttatct cgggcatctt attcctttaa ttttacataa aaaggaggct
    17281 gtgacggttt gtctcagccc ctttctattg aaatcttgca ttcaaataac gttctttatc
    17341 tatttatttc ttgccgtact gggaacttgg tctaccgtac caccaattac ctagtgtacg
    17401 ttgtagccat ggaattaccc agcgatccaa cccaatcgca cgtccggaac cgttcatcaa
    17461 tgcgaaagca acaaaaatga accaaatgtt tacccagtaa aacatgcctg ataagcagaa
    17521 cgtgatcacc aatacaatcg ttgtcgcact gcatagccat gtgaataatc cagcaatcaa
    17581 tgctaatgcc aaggcaattt caaagaatac catgaatttt tggaagaaca ttgctacatc
    17641 attgtttggc atgatcactt gcatgatctt tgtgaaccag tctggtgact tctcaatcac
    17701 agccattggt tcttcaccgt atgcataact caaaccaaaa tgtgcagctt gagtcgctgc
    17761 ttctccggca cttgcccctg ctccgctggc agctgtggtt gcatcaccag cagcagaagc
    17821 accactggca acatctgtta cattgtattc ccatgtccaa gggaacacac tgtttccagt
    17881 gaaccatgat ccttcaccaa accaagtact tggtttgaac acttcaccat tcccaatgat
    17941 ttttttggaa gcttcaacga accaaactaa accatagaaa atacgaagtg gcacactcca
    18001 taagacattg ccgtaacgag aagaatgtcc tcgagtcacg ttgcgatcat ctttgatatg
    18061 gaaaaactca tgcattaaat attggaacat ataataacca gaacgaatat caaagaagta
    18121 tttcaaattc acgatatgtt tcattacgat agctaaaaat ccgcttaaat ggatcttatc
    18181 aaataggtta gctacccccc atttggcacc aatagaaacc ataaatcctt gatagtttcc
    18241 tttaaattta tgcttttctc cgcctttgat cgaagccacg atattcgctg cggctgtgtg
    18301 tcctgtttgt tcagcagctt gaacgatctg cggtgtcggt gttcctggga attcttcata
    18361 aaagactaaa tcaccaatga tatagatgtt tttatcttcg tagccttttg cctgcatata
    18421 ttcattggcg atcaaccgct gtccgcgtgc tgattctaat ccaaagtcag cagcgtcaga
    18481 agttcctttg acaccagctg tccaaatcag tgtgtgagtc ggaatgttgc gtccgtcttt
    18541 caatttgata tgatcagcag ccacttctac gattggtgca ttcaataata attccacgtt
    18601 gtgcttacgc aaatagcgtt cagcttttgc agcatcattt cgatccaaca tattcaagat
    18661 cgtcggcatt gcctctacga ccatcagtgt gatttcgttt ggatctaatt tataatcttt
    18721 ggctaggcga tttttccagt cgatcagttc cccaatcatt tcaataccgg taaatccaga
    18781 accacagaca acgaaagtca gcatggcttt acgcagttca tcatctggtt ccaatgcagc
    18841 ttttgctact gtcttctcaa tatgttcccg aatccttaca gaatcttcaa atgaccaaag
    18901 ggtgaatccg tgtgctttga caccaggtgt accaaagtca ttcggctctc cgcccattcc
    18961 aaggatcagc tgatcaaaag gataggaacc taatttggtc ttgacgattt ttcgttcttt
    19021 atcgataccg attacagtgt ctgtgacaag ttgtacattc ttttgacgag caaacaaatg
    19081 ctgcagatcg tattggattg cttctggttc tacacgtccg ccagctactt catgcaactc
    19141 ggtcatcata gtatggtacg aatgacgatc gatcagagta attgctacat cttctttttt
    19201 cagtttcttt gccaagtatt ttgtagctga aacaccggca tatcctgctc ctacaacgac
    19261 aatattttgc tttgccattg atcatccgct ccttatagac tatctatata atgaatatat
    19321 gataattctt ttagtttgat acttattaca caatctccat atattatata gatgttctgt
    19381 tcttttgtct aactcctttt ggattttttt caaattttaa aataaagcgt tttttcacga
    19441 aatttgctga aagaaaacgt ttgaaatatt acatgggtta tttatcaaaa atagttgact
    19501 tataggctaa aaagattaga atgtataatg aatcacaaag aaaaattgtg gactagaaat
    19561 ggaatggtgg ggtattaaaa tgaagaaaaa acgttttgta actggatttg ccgtattagc
    19621 attttcagct ctagtgttag gcgcttgcgg cgcagatgac aacggaagct caaactcttc
    19681 ttctgaatca tctacagcac aatcttcgac tgcaaaaagc acaacagaat catctgcaaa
    19741 agttgtagct ggcggagatt tgcaagacgg cacatataaa ttagaagaaa aaaattattc
    19801 aaatggttac cgtgcagtct ttgaaatggt tgtaaaagac ggcaaaatca ctgaatcaaa
    19861 atatgataac gtcaatgaaa acggcgaatc aaaaacaaaa gatgcagaat acaacaaaaa
    19921 catggaagca aaatctggca caaacccaga aaaatacatt cctgagttga atgaccaatt
    19981 cttaaaagca caaagcgcaa gcggtgtgga agtcgtaact ggagctactc actcatctga
    20041 aagcttccaa aactacgcac aacaattgat ccaagctgct caagctggta acacagatac
    20101 aatcgaaatc gacaatggtg ctgatttgaa agacggcact tacaagcttg aagaaaagaa
    20161 ctactctaac ggctaccgtg tgcaattcga aatgacagtt gctggcggca aagtaactga
    20221 atcaaacttt gattatatcg acaaagacgg taaatcaaaa caagatgata cagaatataa
    20281 tgaaaatatg aaagcaaaaa gcggtacaga accaaaaact tacatcccta ctctgaacga
    20341 cgaattcgtc aaagcaatgg gcgaagaaga tggctcacct gctaatgtag aagttgttac
    20401 aggtgctaca cacagctcac attcattcat catgtacgca caacaattag tgaatgcagc
    20461 tgaaaaaggc gacactcaaa caatcgaagt agataatatc gtaacagaaa aataatcaca
    20521 taatcatact aataaaagtt ggagggtggg acgatagcca gattaggcag tcgtctcacc
    20581 tttcaaaatg aagagaggaa aaagatgcgg aaaaaactac tattttcctt aatcacactg
    20641 ggcatctctt ttatggtgct aactggctgc agcaactcag caaataaaac aaccactgaa
    20701 agttcagcag ctaccaagat attgaaagat ccatattctg atgaacagtt cttgttaggg
    20761 acttacgttc gtatccgtgt gtatgatgaa ggcaaagaat cagcattgaa acctgccttc
    20821 gatcgagtaa aagaacttgg tgataaaatc acgatcaatc aaaaaggttc tgagatcgat
    20881 gaagtgaatg agcaagctgg gatcaaacca gtaaaagttt ctgatgacgt ctacacgtta
    20941 gtcaagcggg cctacgaata tagccaagat tctcaaggcg gattcgatat ggcgattggt
    21001 gcaatcacac aattatggcg aatcggtttt gatgacgcca gaaaaccttc ccaagaagaa
    21061 atcgatcagg cattgaaatt agtggattat cataagatcg aactaaatga caaagaaaaa
    21121 actgtttatc taaaagaaaa aggaatgatc atcgatttag gtgcaatcgc caaaggttac
    21181 attacggacg aagtagtgaa agtcttgaaa aaacaaggag tgaccactgc catcgttgac
    21241 ttgggcggta atgtctatgt cttaggccac agcccaagag gcgaaaacca agactggaca
    21301 gtcggcatcc aagatccgaa catggctcgc ggatctgttt taggttctat taaagaacgg
    21361 aacaaaacat tagttacctc tggaatttac gagcgttatt tagaagtaga tggcaaaaaa
    21421 taccatcatt tgtttgatcc aaaaacgggt tatccgttcg acaatgatat cgcaagtgtc
    21481 acgatcataa cagataaatc aattgatggt gacgggttgt ctacagcagt cttttcaatg
    21541 ggtgtgaaaa aaggactgga atacgtcgaa agcgaattga ataacgggac ggaagcgatc
    21601 ttcgtgacaa aagacgataa agtatatgtg accgatccaa tcaaagacac cttcaaatta
    21661 tcggaggact cccattacac gatgggtgac cgttctgaac taaaataagg agtacgaacc
    21721 atgacactaa aaatcttttt agaagtagtt gaaatacgaa caaaggtggc tagtgtattt
    21781 ccttttgcgg taggggtatt attctctatc gctttttttc atgagataca ttggctaaac
    21841 acttttttat tttttattgg aatgctagtt tttgatttag cgactaccgc tatcaataat
    21901 tacatggatt atgaaaaggc tcattctgaa gtatataagt atgaagaaaa tgtcattggc
    21961 cgcagaaatc tatctcctgt attgattcga aacatgattt tagctatgat tgcatttgtt
    22021 tttgttatcg gcgttattct aacgatcaat acgggttggc ttttactttt gatgggattt
    22081 atctgttgtt ttattggagt attctataca ttcggaccga ttcctttatc tcgaatgcct
    22141 ttaggggaaa ttttcagtgg ggtgactatg ggccttggaa tttttgccat ggtcgtttat
    22201 gtcaatacat tcggttcaga tgtctatgat ctgacagtca acttttcatc tggacaattt
    22261 gatttggtag gaaatctttg ggctatagca gcaatcgtat tagcttcctt acccttggtg
    22321 tttacgattg ccaatatcat gttggctaat aatttgcggg atcttgaccg tgatattgaa
    22381 aatcatcgct atacactagt tttttatatc ggacgtcctg cgggaacagt cttattccag
    22441 ctattgatgt atgcttgcta tttcgtgatt ttaatggggt tgttgagcgg tatctatgaa
    22501 tggccgatct tggtcacctt cttgaccttg ccaattgtga agaagcattt ggatcaattc
    22561 aagcaaacgt taccccagcc tatcagtttc agctattcca tcaaaaatat ggtcttattc
    22621 aatgccagtt atgtattggg gctattacta acaatcatta tgcaagccat ttaacagcat
    22681 gaagaggttg tgaaaaaact gccctgcatc aagtaataag aacagccaaa taaaaatagc
    22741 tccttatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    22801 tatcaggagg ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
    22861 ggctttccac atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg
    22921 tttatcagag gttgtaaaat cagtgttttg tcctgagtat tagagaaaca aatggaaaaa
    22981 tagttcctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacta
    23041 tttgttcgga aaaagtgact aactatgaca caattttttt cacagtccct tttttcactt
    23101 gatttgattc gtaccagcga acttgacgag aactttcttg gtttggtaac tatatggtat
    23161 tcttttaaca tcctcgtttg cttcttctaa tttatttata caaagtcaca cttttttaaa
    23221 catctgttct tcgatacatt tctatttctt ttaaacatcg ataccaatat tttgaactta
    23281 agagtgcata ccaaatggat tcatttactc ttcgtcagtt acgaagataa tcaaaatctc
    23341 caacttccct acttatttcg ttttgagaat tatcgaagca atcttacata aataactaaa
    23401 aaacaaaaaa ccagaaaatc agtatactga t
//
LOCUS       DAGCWU010000025        22804 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.025, whole genome shotgun sequence.
ACCESSION   DAGCWU010000025 DAGCWU010000000
VERSION     DAGCWU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.025"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(482..2374)
                     /locus_tag="MBZ71_000808"
     CDS             complement(482..2374)
                     /locus_tag="MBZ71_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428780.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2371..4098)
                     /locus_tag="MBZ71_000809"
     CDS             complement(2371..4098)
                     /locus_tag="MBZ71_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428781.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4114..4764)
                     /locus_tag="MBZ71_000810"
     CDS             complement(4114..4764)
                     /locus_tag="MBZ71_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBT4428782.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4968..5264
                     /locus_tag="MBZ71_000811"
     CDS             4968..5264
                     /locus_tag="MBZ71_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBT4428783.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5456..6076
                     /locus_tag="MBZ71_000812"
     CDS             5456..6076
                     /locus_tag="MBZ71_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBT4428784.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6168..7334)
                     /locus_tag="MBZ71_000813"
     CDS             complement(6168..7334)
                     /locus_tag="MBZ71_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4428785.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7346..7699)
                     /locus_tag="MBZ71_000814"
     CDS             complement(7346..7699)
                     /locus_tag="MBZ71_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBT4428786.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7827..8849
                     /locus_tag="MBZ71_000815"
     CDS             7827..8849
                     /locus_tag="MBZ71_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBT4428787.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8868..9029
                     /locus_tag="MBZ71_000816"
     CDS             8868..9029
                     /locus_tag="MBZ71_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428788.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9288..9722)
                     /locus_tag="MBZ71_000817"
     CDS             complement(9288..9722)
                     /locus_tag="MBZ71_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428789.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9832..11691)
                     /locus_tag="MBZ71_000818"
     CDS             complement(9832..11691)
                     /locus_tag="MBZ71_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBT4428790.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11809..12968)
                     /locus_tag="MBZ71_000819"
                     /pseudo
     CDS             complement(11809..12968)
                     /locus_tag="MBZ71_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13275..14699)
                     /gene="araA"
                     /locus_tag="MBZ71_000820"
     CDS             complement(13275..14699)
                     /gene="araA"
                     /locus_tag="MBZ71_000820"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBT4428791.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14715..15413)
                     /locus_tag="MBZ71_000821"
     CDS             complement(14715..15413)
                     /locus_tag="MBZ71_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBT4428792.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15370..17031)
                     /locus_tag="MBZ71_000822"
     CDS             complement(15370..17031)
                     /locus_tag="MBZ71_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBT4428793.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17249..18649
                     /locus_tag="MBZ71_000823"
     CDS             17249..18649
                     /locus_tag="MBZ71_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBT4428794.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18901..19980)
                     /locus_tag="MBZ71_000824"
     CDS             complement(18901..19980)
                     /locus_tag="MBZ71_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4428795.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20066..21025)
                     /locus_tag="MBZ71_000825"
     CDS             complement(20066..21025)
                     /locus_tag="MBZ71_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBT4428796.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21037..22548)
                     /locus_tag="MBZ71_000826"
     CDS             complement(21037..22548)
                     /locus_tag="MBZ71_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBT4428797.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22570..>22804)
                     /locus_tag="MBZ71_000827"
     CDS             complement(22570..>22804)
                     /locus_tag="MBZ71_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428798.1"
                     /translation="FLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLG
                     FVLLIFLFPPFIFIFAGGVWKLVSIILTIGNER"
BASE COUNT         6502 a         4811 c         4051 g         7440 t
ORIGIN      
        1 tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
       61 aaaagtcgct tagtttcgag tagtaagaaa aattttgtga aaatggcttt ctacattttt
      121 gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttacc aagaggttgt
      181 gagcctgccg tttaactccg agtcacaagg agcaatttca aaaaacagct tctcaggttt
      241 gttgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta aggaagaggt
      301 tgtaaaatca gcggtttgac ctgagtatta gagatacaaa acggaaaaat agcttctcat
      361 attttgccgt tttttgagct aataccgaag gacagctgat tgaacaccgt ttattcggaa
      421 aaagggactg tgacacgact tttgtcacag cccctaaaaa aaataataaa tttgtactat
      481 tctatgaagc ttcttcttca gaaaactgac tgttataaag atctgcatag aatccgtctt
      541 tttccatcaa tgtatcgtgg tttcctgttt ctacgatcga accatgattc atcacaataa
      601 tgttgtctgc atcgcgtatt gtagacaagc ggtgagcaac aacgaaactt gtccggtttt
      661 ccaataggcg gttcatcgct ttttgaatca gtatttctgt tcgtgtatct acactagatg
      721 ttgcttcatc aaggatcagg acatctggat ttgctaagaa tgcccgcgca attgtgatta
      781 gctgacgctg cccttgcgaa atattgcttg catcctcgtt taatacagtg ttatatcctt
      841 ctggcaattt acgaacaaaa tcgtctacgt gagcagcttt agcagcctca atgatttgtt
      901 cgtctgttgc atcgtcgttc ccatatttga tattgtcata gatacttcct gtaaacagcc
      961 aagtatcttg cagtaccatt gaaaactgtg cgcgcaattc atcacgtgaa agatcacggg
     1021 tatctacacc atcgtaacga atactacctc cagaaacatc gtagaaccgt tcaagaaggt
     1081 tgatcagtgt cgtcttccct gcaccagtcg gacctacaat agcgaccatc tcaccaggtt
     1141 tgacatccaa gttaaaatca gtcattagca agttatctgg tgtgtagccg aattgaacat
     1201 tttcaaaacg aactttgtat ggtgtatctt tttcttctgg aaggccagat ttttcattgg
     1261 acatctcttc ttcatccaac acttcaaata cccgttcggc tgaagcaact gttgattgga
     1321 tagtgttcat caaacttgca agttgagtga ttggttgaga gaactgtgtt gtatattgca
     1381 agaacgcttg aacatcccct aatgtcatgt tcccatttgc aacttttact ccgcctagta
     1441 cagcgacgaa cacatagcct agatttttaa cgaaattcat caacggcatg atgattgccg
     1501 aaataaattg agctttccaa ccagcggcat acagattctg attttctttt tcaaatactt
     1561 cctggtcttt ttcagtatga ttgaatgttt taacaatggt atgaccacca tatgtttctt
     1621 ctacttggtt attcaataaa cctaaacttt tttgctgatc tgcgaaaaac acttgtgatt
     1681 tcggcgctac gatcatcacg atgatcaagc ttaaagggat catcaataac gcgactaacg
     1741 tcaattgcca gctgattgaa agcatcatca cgatgacccc aatcaatgtg actgcactag
     1801 ttacaaattg agtcaaattt tgctgcagcg tgcttgcgat gttatccata tcattgattg
     1861 ctcgagacat gatatcccca tttgtatgtg tatcataata ggaaatcggt acacgattca
     1921 ttttggcttc caaatcatga cgtaattcat aaactgttcg ttgggaaaca cgtgtcataa
     1981 taaattgctg taaaaagttg aatacagcag aaatcaaata catcacgatt actgtcgcaa
     2041 tgatctgcgc gattttatca aagtctatcg gaaattttgt tacttgttgg ccttgttgca
     2101 tctgttgagc gccagccatt acacctttga atatttctgt ggttgctttc cccaagacct
     2161 ttggtgtttg gatttggaag atcacggctg ctattgctag tacaaataca gcgatgatcg
     2221 caaccatacg tttagacata tagccaaaca atcggcgtac cgttccccag aaatttttag
     2281 gcttttcacc ttttacaccg agatttctgc caggtccacc atgtcctgga ccgcctgctg
     2341 gtccatgaga gggacgtgtg tttgttttac tcatgctaag tcctcctctc ttagttgtga
     2401 atgaacgatt tcttgataag tttcgtttgt tgctaatagt tcttcatgtg ttcctttgcc
     2461 aaccagtttt ccttcatcta gcacaaggat catatctgca tccattactg tacttactcg
     2521 ttgagcaact aaaacggtga tactttcttt catattcgtt ttcaatgctt ggcgaagatt
     2581 ggcatccgtt ttgaaatcca acgctgagaa ggaatcatca aacacataga tatctgctgt
     2641 tttgactaat gcccgtgcaa ttgccagacg ctgtctttgt ccaccagaga agtttcctcc
     2701 tccttgttcc acatggctat ctaagccatc ttccatctct gaaacaaaat ctttagcttg
     2761 tgcgatttct aatgctttcc aaatcatttc atctgttgca tccggcttac catactgcat
     2821 attgtctcgg atcgttccag agaacagaac tgctttttga ggaacaaagc ccattaattc
     2881 tcgcaaatta tattgagtca tgtcactaat gtccgttcca tttatttgga tcgaaccaga
     2941 ttcgatgtca tataatcgag ggatcaaatt gactaaggtc gttttacctg aaccagtccc
     3001 ccctatgatt gcgacgatct cgcccgactt ggcttggaaa tcaatatctt ccaatgcaag
     3061 tttttctgct ccatgatatc gataattgac atgattgaag gccaatgttg cgttttttcc
     3121 agcaaaagaa actgttttag gattttcagg gtcatggata ccgatttttt catccaacac
     3181 ttcattaata cggtcagctg aagcttgtgc acgaggcacc atgataaaaa tagcggaaag
     3241 catcataaag ctgatcaaga tctgcatcgc ataagtcata aatgcaatca gattaccgac
     3301 ttccaaagtc atatctgcta tgtagtgtcc accaaaccaa gtgattgcta cgtttgtacc
     3361 gctcatgatc aatgtcatca ttggcatcat caatgcgacg atcgtattaa ctttgattgc
     3421 cgtatttgta taatctttat tcgctaaatc aaaacgattt tcttcaaagc gtgtcttatc
     3481 gaatgctcga atcactctga cccctgtcaa tccttctcga aataccaggt ttaaacggtc
     3541 agttttcttt tgcatacttt tgaataatgg aacagcaaag tacatgattc cgccgatcaa
     3601 aataatcatt actgggatga caactaagaa gatttttgtc aactgatgat ctttttgata
     3661 agcaagtatg ctcgcgccaa tcaacgtgat tggtgcattg atcatcatac gtaaaaacat
     3721 ttgtgtaacc atttggattt gagtcacatc attggtcgtt cttgtgatca aagaggccgt
     3781 tccatattta tcaaacgcat cttttgtcag actctctgat tttttataga catccgtacg
     3841 caattgctta cccagtttct gtgattcacg agtagcaaaa aatgtatttg cgatagcagc
     3901 taagatactg atcaaagaaa agccgatcat cacaaaacct gtatgccaaa tataatcgac
     3961 atctccttgc gctacccctt tatcaataat attggaggta agtgtcggca aatataaatc
     4021 tgccagtact tggatcacca tgaaaactgc agctgcaatt gctgaagtca aggagatccg
     4081 ttttacgatt tttagcatct agttcccttc tttctattta tatactccgt ttttcagcca
     4141 agaaagtttc aaattgaatt cactgattgc ttgctctact gagtaatctt cagatgttga
     4201 aaataagcga tacgaatgcg caattgttgt aaatacagta ttcatcacta attggatcag
     4261 taattccaat tcatgatcat tttttacttt cagcttcgtc cgatcgatca attcaagcaa
     4321 ctcttttgca tcttgtttgt gttctttatg tggatcgtga ggcgtttttt ggcgactcgt
     4381 atgaggagcg acttttcttg aggcacggta atccatgttc ataaacaagt tacgataaaa
     4441 agaagcattc tcgccttcca aaatctcata aatcatttta gaaaaatagg attcgaatcc
     4501 tttgaataga tcaccatcgg attctttcat tagttgtaac atatctttag agctgtcacg
     4561 tctcattgtc tcaaagtaat aaaaataaag atcctcttta tcttcaaaat actgatagaa
     4621 actgcctcta gggatatccg ctaatttgac gatattagca atcgaagctt cttttaaggg
     4681 tactctagaa aactcgattc tcgcagcctc gattagtctt ttttgttttt ctttaggcaa
     4741 atggaaaaat gtctgtgtcg gcatatggat tctcctttct ttcaagatga cacattgtca
     4801 tatgacgcta tgtcattata acacctccta cctgttttgc aagacatttg catagttttt
     4861 ttcattatca tccgatacat taaaaaagtg ataagaggaa tccttccatt tgaattaatg
     4921 agagttgccc attaaaatag ccacatacta aacaaaggat gagataaatg gcccaatcaa
     4981 ttagtgtcca agacttcaat aaattgcctc tcgatgctga cagcatcgtt ttagatacaa
     5041 gaaatacctt agattacaca aaaaaccact taagccactc gctttctatc cccgctgcta
     5101 tcttgccaag acaattaaaa aatctagatc caaagaaact ttattatatt ctaagctata
     5161 caggcaggcg atcagaagca ttagccgatc aacttactgc taatggtttt cgtgctgttt
     5221 atatcattgg tgggatggaa gctcttcagt cgagtgttgc ttgaatattg cttgttagat
     5281 tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt ttataccctg tcttttttta
     5341 gttatttatt cttcaacctt atcaatatga cttgatagcc ttttcttttt cgagtaaact
     5401 gattttgtta gtcatagtta taacaatcat agcaataaaa ggagaaggta agcatatggc
     5461 atctgtttta gtagtcaaag gtcatcccct aactgcagaa gaatcgcgca cagttaaggc
     5521 attgacgtct tttttaacaa gttataaaga aaatcatcca gatgatgaag tgactgtttt
     5581 ggaactttat cgcgatgata ttccggaaat cgatgaagaa ttattatcag gttgggaagc
     5641 attacgtgct ggtgcagaat tcacttcctt atcagaaagt caacaacaaa aaattgctcg
     5701 tttcaatgaa ttgactgatc agttccttgc tgctgataaa atcgttatcg caaacgcctt
     5761 atggaatcta aatatcccaa caaaattaaa agcttggttc gataccgtca atgtagctgg
     5821 aaaaacgttc cgctatacag aaaacgggcc agaaggatta gtgacaggta aaaaagcact
     5881 gcacatccaa tctaatggcg gtgtatacaa cggacaagac tttgcctctc aatatgtaaa
     5941 aggtatttta aactttgtag gcatcgatca agtagatcag ctattcatcg aaggaatcga
     6001 ttatgaccct gaccgtgcag atgagttgat gcaaaatgca ttagataaag ctgctgcttt
     6061 aggtaaatca ttttaaaaat tgagataaat gcatacaaaa gagacagagg aaaaaatctt
     6121 caaagcagca tttgtgcctt ggatattttc ccatctgtct ctttttgtta ttctttctgc
     6181 tttttcaacg gattttcttc ataaagagaa gagattgttg tgtaccattc aaaaacatga
     6241 gtaatcacta ctttagctgc tgcataaaca ggaatcccca agatcacacc gatgattccg
     6301 aacaatttcc cagaagtcag cagaactaat aaaatcgtta ctggatggat tgctaactga
     6361 ttccctagta ctaatggaga gatcaagcgt ccttctattg tctgctctac tgcaaaaacg
     6421 atcagtactt tgatgatcat gaatggacct gctacaatgg ctaaaaagat agccggaatc
     6481 atcgctaaaa atgatcctaa gtaagggatc aggtttaaaa aacccgcaat aatccccaac
     6541 gtgacagcat actctaaacc aatgaccgca aaaccaatca taaacatgat tgccacagca
     6601 aaagccacag ttagttgtcc tcttatataa gaagaaactt gactattcat ttcactcagc
     6661 acattcaacg tcggtttttg catgcgtgtc ggtaaaaatt tgacaaaata aggtgctaat
     6721 tgtttaccgt ctttcaatag ataaaataag ataaacggca tcgtcagcag agccactaaa
     6781 atagtcgcta ccgcgccgac aaaacttcca agactctgaa ctgtactctt agatatatct
     6841 tggatcatat cgcctgccga attcatgaac ttatcacctg ctgtttccaa ctgttcgcga
     6901 aattggttga acagcgggtc ccgcaaaatc tcggttaatt tattatcaat cgtatctaca
     6961 tattgaggga agttactgat gaagctcact gtttgttcct gaatcttagg gatgatcact
     7021 acgctccccc atgcgattag tgcaacaacc aaaacaaaca gcccaatgat actgtaaagc
     7081 cttggtactt ttcgcttttc aaagtaatca actaccggat tcatcaagta atacaagatt
     7141 cctgctaaaa tgataggcag acctacgatt gctaaaaatt gccagattgg tgcaaacaaa
     7201 tatgaaactt ttgtaaataa aaagacgatc agcaatatta acaatacaac aagtaaggct
     7261 gtgactactt gattatttaa aaaccagcgc caaaaccacg ataccggtct gttcttctta
     7321 ttttttcctt ccataattcc tcctgttagt tataaaaaat ttggcttccc aaaccaatag
     7381 caggcagtct atgcggatga agatagattc catttgcaat ttgtccttct tctggacaag
     7441 catcaaaaat caatgatacg tgagctactg tctttaaatt cccattggcc atcggaccga
     7501 cataatcgat cgtatatacc tgatcatcaa aagcaattgt tccgcctgct ttcaaagaaa
     7561 aaggttcgtc ttttgatact tcttgaatca ccgaatattt tcgtaaggca gctgttgctg
     7621 ttttatcgaa caaaatcagc atgggttctt ccttgtttat tgcttctgtc ccaatttctg
     7681 taatctttgc ctgaatcatt gcactctctc ctttaattgc tttcaaaaac agtataacac
     7741 attctcattc tttagccgtg tggaataatt atgtgataga ataaaaacga aaactatttc
     7801 attacaatta ggaggaatca gctcatatga tcgaaaagat aattttagga acctatacgc
     7861 gtcgcgaaag taaaggaatc tatacgatcg accttgatac agaaaaagaa gaattagcca
     7921 acctttcatt agctgttgaa gaaaatagcc ctacctatgt agcaaaaagt aaagctggca
     7981 atctttacac agttactagt gtggatggat taggtggtgc cggtgcgtac gataaggatt
     8041 ttcatttttt gaatgcggta acagagtctg gcgctccttt gtgttatgta gcggttgatg
     8101 aagcacgtca attactatac ggggcgaact atcataaagg tgaagtaaat gtctaccata
     8161 tattaggaga tggcgaactg aaagcagcag attcaatatt ccatcaagaa gcgacaggtc
     8221 ctcataaaaa ccaagatcat gcgcatgtcc actatacgga tctgactcct gaccaacgat
     8281 tagtcgtatg tgatcttggt acagatcgtg tgtatactta tgatgtgtcc gaaaacggca
     8341 aattgaatct tgtaactacc ttcactgcag agccaggaac aggccctcgc catcttgtct
     8401 ttcatccaaa tggacaattc gcttatttat ttggtgaatt agacagcaca gtccgcgtgc
     8461 tttcttataa taaagaaaca ggtgcttttg aagaattaca gaaaatatcg accttgccag
     8521 aagaattcga cggtgaaaat ggaggggcag ctatccgcat ttcaaatgat ggcaagttcc
     8581 tttatgcttc taaccgagga cataactcga ttgctgtatt cgcagttttt gaagatggtt
     8641 cccacattca aaacaagcaa atcatctcaa cagaagggga ttttccccgc gatttcgcac
     8701 tagatccaag caataatttt gtcgtgtgtg ccaatcagaa caccgataat ctgacattat
     8761 atcgccgtca tcctgaaaca ggattactgg aattactgaa aaaagatgtc tatgtaccag
     8821 aatgtgtctg tgtttatttt gatcgttagt aagaaaagga gaatgaaatg aatcagcaag
     8881 acgtattaga cagactacgg gaagaactaa atattccttt ctttgatggg aaaatcgaag
     8941 ataaagagta tagcgaagag gaatatcaga aactcaaagc cgatttacag aactactttg
     9001 aacaatatgt acagaatgta gaaaactaat cagctttcca tactatctga aaagaacatc
     9061 aggacaagac ttttccgtct atcctgatgt tcttttctac aaataaaaaa ccgccgagat
     9121 ggttagactc gacggaaaag gagtatttac tcgattttga gtaagtacaa ggttgttgtt
     9181 ggttataggt atgtcattat cataaaggat tttgatcact ctatcataaa ggtaatatta
     9241 catttatatt agataactct agtttttatt gttatcaaag atttctttta ttttttcaac
     9301 aacaaattag tcattttttg gatggcagct gtcaattggt ctgtcgcttg gtcttgtttt
     9361 gcttcagatg tattgaatag atcttcatct acccctgatt caagatcgat actttggatc
     9421 atttcttcac agcctgctac atatgaaaga taagcttttt caaaattttt atgaatgccc
     9481 attgcttttg ctggtggacg caaagtactg actttcataa gcattgcccg atattttttt
     9541 gttccttctt caaataacag gatgatctct tcaagagtcg atttgtccag ttcacttgtt
     9601 tttccttcat caattgcgtt tctgatcact tcatatttcg gatgcattcc ttctccgatt
     9661 gcttctgttt ccttgacgat ttgatcgatg gactgcacat aatagccaat attcggtaac
     9721 atgttcgttt tcctactttc tccattgttg ttattattta ttctacttgt ttttatcctc
     9781 aaatgacaag tacttgaaaa aatttaatgg tttctataca gctcgccatt cttattcgtg
     9841 ctcaaagaaa ccatctgcta aactatattg tttccctgcg acaatgtcat attggatcaa
     9901 ttgatattcg tcgtaaatct cttgttgcga gcgattgaat tgcgcttctt tataccactg
     9961 tccgttttga tagtacagtt ctcgttcaaa cgccggaagt tcctgttcta atgataaaag
    10021 cagctgataa aagccatttg ttgtctgatt cgtctgttcc attagattcg ctgcaaaata
    10081 aaacggactg gtgattgcat cgtgagtcgt ttgcttttct aattctcctt tactgtcgaa
    10141 cataagaaat tgtgtttcat gcaatgtttg tttgtcattt tttgcttgga tcgtatctga
    10201 atagatccct ggaagatgat ccccccaaaa aacaactaaa gtccttctag aaagattctt
    10261 caattcttcc gtgaactgct tcagtgccgt gctgctatag gaaatatctt gcagataatt
    10321 ctctaagtca agtgtcccac ttccatctga agtttttgca ggatagtcta gattatcata
    10381 ctttccatcg tagggcatat gtgtctgcat cgtgactaaa tgaatgaact gaggtgtttt
    10441 ttcttccttg agaagtgtca ttacttcttt gtaagctgac tcatcagaaa tatatggatt
    10501 attctctatc gtatccgtat aggtcatcgt acgctcgctg atgaactggt caaatcctaa
    10561 tgtctgataa acatcctctc ttttatacat cgaagtattg tatggatgga tcgctgtcgt
    10621 ctggtatgat tgtgcgttta acgctgaaac gatagaagga agctgatcca tttttggtac
    10681 aagcatcgtg taaggggtgg tgagttgcgc attaaataac gccatagaaa aaccagtcaa
    10741 tgcttcaaat tcgatatttg ctgttccacc gccatagttt tgagaaagca tattgccact
    10801 atatgtttga tcagctactt cataataatc tgccaatggc tcaccagata cttctacgcc
    10861 gtttaatcga ctaggatctg agaaactttc actcatcacg aacacgatgt tcggcgactc
    10921 ttcctctaca gttttatttt tttcatcggc tagcttctgg tatttttcag tgatttcttt
    10981 gatcttttct ttcgaatatc cttcaggttc atccattggt tctaccttca gattgtacaa
    11041 aaacccaccg ataaaaccag tgttatagta gttcattttc tggctgtaag gtatccatag
    11101 tgccgtttta ttatatgctt ttcgcaacaa attatcggga ttgttgaaat tactgatata
    11161 ccctagcagt ccaattgcca caagcagtgt cgttaatcgg atcaattgga atcgccttcc
    11221 ttttttcagg cttcgaaaca tgtaccagca aaacaatcct atcacggttc ctgcggcagc
    11281 taagatcact gtaaacagca tagtgccggt catatctttc agcaatccga tttcagtgat
    11341 catcttcaaa tcgtctggat aaatcggttc ttgccgataa ctcattttca tataatttgc
    11401 aaaacctaat acaccgatgg ctacgacata cgtaagggaa cctaaaggga cagatcctgc
    11461 tagtgcaatc agaaaaataa gaataatcag caagacgaga caggctaaga agaatttttc
    11521 cgtatgccaa gaaaaagcaa atttcaacgc aaggtctacg gatagatcat tttgacacca
    11581 ctgcaaaaat aaattactaa aaacaacaag aaaaaagata ctgatgattg ctgctgttcc
    11641 ttttaaaatc tttttctgtt tggtcgaaag tctgcttata tatcttttca aatgcttgct
    11701 ccttcatttc tatcaataaa agaatgttgc ttctcttatt ttagttgtac gaaattcgaa
    11761 agtaaagaac ggaaagaaga attaatgaaa tccaaatatt ctctttcttt atggcctttc
    11821 tggtttcttt aataagcgga cctttcgtac tccagaaatt ttttctagct gttgtttcac
    11881 ttgattgagc tgttcaaaat cagtactgtc tatgtcaatc aatatgtaga catgctcatt
    11941 cttatggtta cgcgatatat ctgcgatcgc tagctcgttc tcattcagca gagaaaatat
    12001 ctctgctaat atattggttt tcttttgata aaatatagtg aatcgaaacg gcgcttggaa
    12061 aaacattcgt gcggatggat aattgaccgc ttctctaacc gtaccgaaca aaagataatc
    12121 ttttaaggct tcaactgcca aacgtccgct gtctgccaaa gcttcctgag tcgatcccct
    12181 agatgaggca acataagtat acggtcattt tctaagaact cttcttctgg aaaatcagtc
    12241 aagtatttag ccagatgttg tttttccaat gcttgcataa cagcatattt atcaacaatt
    12301 tcacttcttc ctacattgat aagtacagca tcttttttca ttttttctat attcgcctga
    12361 tcgatcaatc cttttgtatc ttctgtcaac ggcagcatga ctacgatgta atccgaggta
    12421 gaaagaactg tttctaaatc tgcttgccga acataatcca actgggcatc gcggatagaa
    12481 taacccaaga catccatccc taagctatag caacttagcg ctacttttgt gcctatcgct
    12541 ccaagaccaa gaagaccgat ggttttgcct tgcaattctc gtcccacata agcacttctt
    12601 ttgttttctg cctgttctaa aatattagga cctgttagtg tttgtaccat acgacttgct
    12661 tcgattatcg gacgggaaga taagagcaaa cagcacaaaa caagctcttt tactgcatta
    12721 gcatttacac ctggtgtatt catgacgatc gtcccgtttt cagaagcttt ttcaacgttg
    12781 atcgtgttca ccccaacacc agctcttgaa attgctagca aatctggtgt aagccattca
    12841 tctttcactt tcgtactacg aataactatt ccttcggctg gcaaatcatc tattattttg
    12901 aaatattccc cttgtaactt ttctatctct tgttctggaa tagcatcaat caaatgaacg
    12961 tgatacataa aaccccctcc ttactaacaa gtatctcgaa atttctgcaa ttagacaact
    13021 gtttaccatt tgacttccta gaagcaaaaa gagcagttag agcagagtag gctgagtgat
    13081 aaaaataagg ctatgacaag ttactgccat aagccttatt ttcgagagca aaatattgag
    13141 ggtgagatat cttgataaat ggtgttccgt tagctgacat tgataataag actaaatttc
    13201 aaaaaagttg aaaagcaaat ttcgtcaatc tcttcttatt tcttaaaact aagtggcttt
    13261 ttcacaacct ctttttatct aataaccact gtttccaact caactaattt tgcccacgtt
    13321 tcaatttgat ctgttgttag atttagcgag acaacggtat gatgaccgcc acctgcctga
    13381 atccatgaat ggacaccttc atggaaattc ggtttagttt tccataacac acgtgcaaca
    13441 ggtaaattag gtgcttcagt cgttggttca aaggcttcta cttcattgat cagcagttta
    13501 taatgcgtac cgaaatctgc catcgatacg actacccctt ctcctgcttt cccatcaaat
    13561 acaagacgcg ccggatcttc tctatctccc attgataaag gagaaacaac gattcttggt
    13621 ttcgtcgcag caagcgtcgg atctacttcc atcatatggg attccaagat tgcttcttgg
    13681 ccactagcta gctcgtaagt ataatcttcc atgaatcctg tatctttccc tcgagccatg
    13741 attttcagca accgatcaat cgctgcagtt ttccagtcgc cttccccagc aaaaccgtat
    13801 ccttctgcca tcaaacgttg tacagctagt ccaggtagtt gtttcattcc atacaggtct
    13861 tcaaaattag tcgtaaaagc gttatagcct ccttcttcta agaaacggcg aataccgatt
    13921 tcctgctgag cttgtacttt tacatgttcc tcccatgtct tttcttcata atcaccatag
    13981 tcgaaggtat acaattcctt gtactcttca aataattgat cgacttcttc gtctgatacg
    14041 cgatcgatta cttgtacaag atctccgata ccaaaataat caacagtcca accaaattga
    14101 atctgtgctt ccactttgtc tccttctgtg acacccacgt ttctcatgtt gtcaccaaaa
    14161 cgagccactt ttattccaaa actttcatta taggctactg caacatccat ccaatcagca
    14221 atctcttttt ggatctcagg tcgtttccag tagccaacga cgattttatt ttgtttattt
    14281 aatcgagcat tgatgaaccc atattcgcgg tcaccatgag cggattgatt taaattcatg
    14341 aaatccatat cgatcgtttt ccaaggaatg ctttcgttat attgcgtagc taaatgaagc
    14401 aatggttttt gtagaagctt tgtcccgcgg atccacattt ttgctggtga gaaagtatgc
    14461 atccaagtga tcacacctgc tacttcttct cgataattga cctctttcat gatttttgtg
    14521 atcgtatcag cagtaactgc taattcttga aggcggattg gataaggcaa ttggccattt
    14581 tcattcagtt catcgaccat ttcttgtgca tgtttcttta cttctcgcag tgcttcttcc
    14641 ccatataaat gctgtgatcc tacaacaaac caaaattctt tttctccaat attcaacata
    14701 attagccact ccttttattt gttttgtccg taatacgcat tttttccgtg ttttcgtaaa
    14761 taatgtttat ctaaaatccg ttgaggcagc tcttctgaaa atgaattgag ctgtctggta
    14821 aataaattca tcttgcatac ttcatctagt acgactgcgt tcattaccgc actttgcgca
    14881 tctttccccc aagtaaatgg accgtgtccg tgtaaaagta cccctggaat tgctaaggga
    14941 tcaattttgc gttctttgaa tgtttctatg ataacttttc ctgtttcata ttcgtacccg
    15001 ctgtcgattt cctgttgtgt caaaaatcga gcacaaggta ccgatccata aaaagtatct
    15061 gcatgcgtcg tccccatcgc aggaacatcc aatcctgctt gtgcccagat cgtcgcccat
    15121 gtcgagtgag tatggacgat tcctccgatt tgaggaaatg aacgatataa aacagcatga
    15181 gtaggcgtat ccgaagaggg attgagttta ccttcaatga tatgattgtc caagtccaca
    15241 acgaccatgt cgtctgctgt catctcttca taactgactc cgctaggttt gataacgaaa
    15301 tatcgtgttt caggatcaaa agcgctcaca tttccccatg tatatttgac gagtccgtgt
    15361 ttaggcaagt caagatttgc ttggtatact tcttctttca gttgttccag cattttctat
    15421 cactcctttt tcttgcaatt gatgaatcgc tgttttttca atgtcgatcc cctcacggta
    15481 acgtctcata aatatctgat agcctgctat ttcttctgct gaaggtgtga tggtgattcc
    15541 ttggtcttgt ccaaacacgt cttctgccaa gaagttttct aaagacttcc catgtcgatc
    15601 catcatatag gctgctaata acgcgattcc ccacgctcca ccttctccgg ctgtgtccat
    15661 cactgtaact ggtgcctcca tagcagatgc tagaatccgc tgtccaactt ctggtgtttt
    15721 aaaaatcccc ccatgcccca ctaaacgatc aatttggaca ttctcagatt tcaatatatc
    15781 cattccgatt ctcattgcac caaaagcact ggctaaatgg atccgcatga aattcgctaa
    15841 atcaaaacgg ctacctggtg tacgtacaaa taatggcctt ccttcattta ctcctgtaat
    15901 gttttcaccg gaaaaataac cataagaaag taatcccccg caatccgtat cgccttctag
    15961 cgctttgttg aataataccg tgaataattt atctctgctt atctccatac ccaaagaatt
    16021 ggtaaattct tcaaaaatac tgacccaagc attgatatcc gaagagcagt tattcgtatg
    16081 aaccatgcct actagttctc ctgaaggtgt agtgaccatg tcgatttccg gatggacatt
    16141 ttctagttct ttttctaata cgatcatagc gaaggcggaa gtaccagccg aaatgttccc
    16201 tgtccctttg gctacactgt tcgtagcgac cataccggtt cctgcatccc cttctggcgg
    16261 acaaaccggg attcctggtc tcaaatttcc cgtagggtcg atcagataag cccctgattc
    16321 gcttaacttg ccagcttctt ctcctgctac tagaacttta ggcaaaatgt gatccaagtc
    16381 aatcgctacg tgcgcttttt cgattaactg accaaaaata tttttcattt cttgatggta
    16441 ggttttcgtt gaaggatcga taggaaacat gcctgatgca tctcctacgc ctagtacttt
    16501 ctcaccagta agctgccaat ggacataacc tgcaagcgtt gtcaaaaaat taatttcttt
    16561 cacatgttct tctttattca aaatagattg atacaaatga gcgatactcc atctctgcgg
    16621 aatattgtat tgaaattttt ccgttaatat tcgttctgct tcttcagtga ttgtatttcg
    16681 ccaagtacga aatggtacta gtaactgttc cttgctatca aatgctagat aaccgtgcat
    16741 cattgcactg aacccaatcg acccgattgt cgtaagattc tcgccataga tttcgaatac
    16801 ttctgctgcc atttttcggt aactgacgcg cagtcctttc caaatatctt ctaatgaata
    16861 ggtccagata ccattttcta gtttgttttc ccactcataa ctaccagcgg caataggagt
    16921 aaaagatgaa tcaatcaata cagctttgat cctagttgat ccaaactcaa tccctaaaga
    16981 tgtacgacca tctatgacat ctaacatact ttgcgcattt acttccttca taatgattta
    17041 actccctcct tctcgtcaaa cctgatcaaa ccgtcttttt agtagcgctt acaatgatta
    17101 ttgtatactt ttgtacgtac atgtcaagca gttatttaca agaatacaat aaaaaacact
    17161 tgttttgtac gtacataagt tataaaatga aattgcttac aaaatctgat caaccgcctt
    17221 ctcgaaattc tagaaggaga gaatacttat gaaaaaagaa aaaaaagttt cctcatcgtt
    17281 tatttatttt tttggcgcat ttggcggcat tctatttgga tatgatattg gtgttatgac
    17341 cggcgcctta ccttttttac agcatgattg gggtttggca ggtaaagctt ctttgatagg
    17401 atggatcacg tcttcggtca tgttaggtgc gatccttggc ggctctttat ctggtcttct
    17461 ttctgataag cttggacgac gaaaaatgat tcttctttct gcacttattt ttatggctgg
    17521 ttctgtcctt tctgcatctg cccctcacaa tggttcttat tttctgattg cagcaaggat
    17581 tcttctaggt ttagctgttg gcgctgcatc tgccctagtt cctgcttata tgtctgaaat
    17641 ggctcctgct agactaaggg gaagattgtc agggatcaat caagtgatga ttgcttctgg
    17701 catgctcctt tcctatgttg cagattattt gctaaaaggg ttgcctgaaa cgatggcttg
    17761 gcgcgtgatg ttagggctcg ctgctgtccc cgcgctgatt ttatttttcg gtgttttagc
    17821 tctaccagag tcaccgcgtt ttcttatgca atctggccgc ttggaagagg caaaaagagt
    17881 attgaactat attcggacac caaaagaagc tgaacaggaa ttcgaacaaa tccagctgaa
    17941 tgtcaaacaa gaaaagacaa ccggaacttc ttggcatact ttgtttttag aaaaatatcg
    18001 ttccttagtc attgccggta ttggtgtagc agtattccag caatttcaag gagctaatgc
    18061 tattttttac tatattccgc tgatcgtaga aaaagcgact gggcatgcag cgagcgatgc
    18121 attgatgtgg ccgatcatcc aaggaatcat tttagttgct ggttctttgc tctttttggt
    18181 tattgcagac aagttcaatc ggcgaacttt attgaaaata ggcggtagcg tgatgggact
    18241 ttctttcatc ctaccagctg ttttaggaac tgtactggat gctcatacaa attcgttgtt
    18301 gattctcttg tttttatgta tttatgtcgc attttattct tgcacatggg ctccactgac
    18361 atgggtaatc gtaggcgaaa tattcccact agctgtccgc gggcgagcat ctggcctagc
    18421 ctcttctttt aattggattg gttctttcct agttggcttg ctttttcctg tgatgactgc
    18481 ttctatgtct caagaaatcg ttttcggcat ttttgggatc atctgctttt taggggtttt
    18541 atttattcaa gagatcgtac cagaaactcg tgggaaatct ttagaggaaa tcgaacaaag
    18601 tgcatctaaa aaaacttatc ctaaacgtat ttctatccat cattcttaaa gaggttgtga
    18661 cagaagtggt cagcttcaag aaataagaag gaaccgctta gtttccagtc acaaggaaca
    18721 atttcaaaaa acagcttctc atgtttgatg aatattgtga cttgtgacca gttggtagga
    18781 gaacaccgtt tagtggagag gtggtgaaag aaacgttcag ccttgagcga tgacaaaagc
    18841 tctcacaaac attgcgacga gtaaccgtag gagcaataag aggcggtaaa aacccgtctc
    18901 ttatttttcg ttttctttca ctgtttcccg ttcaactagt acaggttcat agagaatatt
    18961 tggctgtgcc tgtccttttt caatcgttgt gacgatccac cgtgcggcat cttgtcccaa
    19021 cttttctttt ggatgcgtca atgttgtcaa ttttttattc cctaaatggc ttaaagaaga
    19081 atcgtctgtt cctacgatcg ataggtcatc tggaatatga tagccatttt ctgtcaactt
    19141 atctagcatt aaactagcta tttgatcgtt atagcaaatg atgccatcta aagcttgttc
    19201 ttctttcaac cgttgtactg ttttttcagc gattgtttct cgtgtttccg tggtgaaagt
    19261 aatgatatct tccgtcaaaa aacgcacatt atactgttca cacgcgctga taaaaccttt
    19321 catcctgtat tttccttgaa gatcgtcgat ttttgtgatg aacaacagac gctgatgtcc
    19381 tttttttatc aaatattctg ctgccataaa accgactttc acatcatcta aacaaatata
    19441 agaggcattt aactcttcat aagcagcatt gatcatcacc atcggaatgc cttgttctct
    19501 caaagcaaca taaagtgcta agtttgggtt atactgatta cttttcgtag gctcaacgat
    19561 caaaccatct accccataag caatcatttt cttcaaacac tctttctcca attcgtgatc
    19621 gttattcgta ctggctaaaa gcaatgaata cccagattct cgtaattcat tttcgattcc
    19681 tcggataatc gacggaaaaa tataatcaga gagatatgtg gtgatgacac cgatcgtttt
    19741 tgttctgttc aatggtgttt tttctttctt atagctgtca tctacatatg tgcccgaccc
    19801 tttttcttta cgcagatagc cctcatttac caacacagcg attgcttgcc gaatcgtatg
    19861 tctgctgacg tcatatattt cttgcagctt gaattctgag ggaatggttg tatttacttc
    19921 gtattcctca cgaaggatct tttgttttat ctcttctgca atatcttgat attttgccat
    19981 ttgccttgcc cctttttatt tgtacgtaca tatatcataa cgagtctgca aggtttattc
    20041 aagcaaaaat caaggtttct tgtgctcata aggcatacgg aacaggttca ccaaattctg
    20101 gaaatccttg ctcattccac gtaaagcatt gcgcgttcgc gtgccgatta ggattgtcta
    20161 acggatcccc tttttggttc ttctctggtc gagcatgata gatcaaaata tcaaagcacc
    20221 cgtcttctga aaccgtaaac gagttgtgac caggaccaaa ttgcttattt ttttcagaag
    20281 aaacgaatac tggctccgat aatttatgcc atgattcttt taataaaaga ttcttgtgct
    20341 catcagccca cagcaggccc atggcatagt tttcatctgt tgcactacca gaatacgtaa
    20401 tgaatatttt cccattgcga gtaaggactg ctggaccttc atttactgaa aaaccccgtt
    20461 tttcccattc atactcaggt atagaaagga ggatctgctt cccctttaac gtccaaggat
    20521 tatccatttc ggatagataa agattagaat tcccagtaat ttcaggatct tgttgggccc
    20581 acacataata tagctttcct tgagaagaga aagttgttcc gtctaaactg aaagtttccc
    20641 ttttcgtttt tatttgtccc ttttctatcc aatctgtatt cattggatct atatgctgat
    20701 tttcaagtac gaacatgcgg tgatgatgag attcatcacg tatagtttta tgattgcttg
    20761 cagcaaagta aatataccat tttccattga tataatggac ttcaggtgcc cagatcaatt
    20821 cactcatcgg tccttcttca tgagcctgcc atacaatcag gcttgcagca tgttctaatt
    20881 cattcagtga ctttgccctt cttaattcaa tcacttggta gcctggaacc gatcctgtaa
    20941 aataataata accatctgta tgtttatata cccaaggatc tgcccgttgc cgaacgatag
    21001 gattattata aagtgtcttc atcataacaa atttcctcac attttcttta ttctaattac
    21061 attccaagag tatttttcta tccctacatt taattcgtca tgtttttcta ttgaaaaata
    21121 gtttatttca ggttttattt tttctggttc ttcacgtgta tttttgtcca acagatcgct
    21181 actggttaat gtgtagtgct ctccttgatc tagcaaattg aaatcatgtt caatcgttat
    21241 cttttgttgt tcatttgtgc gattgatcaa gaaaatagtc agctgattgc cgttatctac
    21301 gatcacactg tccacataag gaacattttt tatcttttca caatcataaa cagggcaaat
    21361 gatttgttgt ttcaatactt gacctttagc atatttcgat aagtgttgca tcacataata
    21421 agttggattc ctccatgctt gtcctttagc atctgtcagt attaaaggta ttgtgtttac
    21481 taacaatgat tgacaactaa tctttacgcg atctgcgtgt cgtaaaatag ccagccctaa
    21541 tgaaccaaat aataatgtat cttccatagt aaagtaacac caatcgatgg gactaccgac
    21601 ttcaaaatcc ctatgtcttt tttccggttg ggcatgtaca ttccattcat caaacgctag
    21661 atacattgtt ttctcacttc tcaaatatcc ttttacataa tcacaagtag caacgatttc
    21721 ttcaatttga cgatcgaatc gataagcttt tgcaaggtat gtcgctgtat catcacgctc
    21781 atttttagga ggtttagtca agtcttcttc atcatactta tcaatatagt tatgcaaagc
    21841 taaataatct acattctcat aagcttcttc taatgcgata cgatcccatt cagggtacga
    21901 tggtaaacgt ggcgtagagc ttccaactaa gattgtttca attgaatcat ccactaactt
    21961 catgactttt gaggcttcat ttgctaacct accatattca taagcactct tagcccccac
    22021 ttgccatgga ccgtctagct cattccctaa acaccataat ttaacgccaa aaggcttttt
    22081 ttgcccattt tttattctta aatcactcca gaaggttcct tttggaaaat tacaatattc
    22141 tactaaattt tttgcttcat caattccttt tgttcctaaa ttcaccgtga atatcggttt
    22201 cgcttgtaca tgactgatcc atttaaaaaa ttcgtggagt ccaaaagaat ttggttctat
    22261 acctcgccat gctaaatcaa ttttttttgg tcgttgttct ttcctaccta cagtatccac
    22321 ccaatcataa ccagatgtaa agtttcctcc aggatagcga atgacaccta attctaattc
    22381 ctttacataa gagaggacat cttgtctaaa cccgtcacca tcagctgttt catggcccgg
    22441 ttcataaatc cctgtataga cgacacttcc catatgctca acaaatgagc caaacagccg
    22501 agaactgatc tctcctaccg taaaatcctc tctgattttt actttcactt cttcgtccaa
    22561 cagctttccc tacctttcat ttcctatagt caagataata gatacaagtt tccatacgcc
    22621 accagcaaag ataaaaataa aaggagggaa aagaaatatg agcagtacaa atcctaataa
    22681 caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat actttccatg cactttgttc
    22741 aaaaatttgt aaaaatgtga tatctttgtt ttcagaaatc acatatatcc aggataaaag
    22801 aaac
//
LOCUS       DAGCWU010000026        22641 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.026, whole genome shotgun sequence.
ACCESSION   DAGCWU010000026 DAGCWU010000000
VERSION     DAGCWU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.026"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            138..2351
                     /locus_tag="MBZ71_000828"
     CDS             138..2351
                     /locus_tag="MBZ71_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBT4428799.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            2362..2808
                     /locus_tag="MBZ71_000829"
     CDS             2362..2808
                     /locus_tag="MBZ71_000829"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBT4428800.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            2979..4238
                     /locus_tag="MBZ71_000830"
     CDS             2979..4238
                     /locus_tag="MBZ71_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBT4428801.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(4327..5274)
                     /locus_tag="MBZ71_000831"
     CDS             complement(4327..5274)
                     /locus_tag="MBZ71_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBT4428802.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            5668..6960
                     /locus_tag="MBZ71_000832"
     CDS             5668..6960
                     /locus_tag="MBZ71_000832"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBT4428803.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            6975..8744
                     /gene="aspS"
                     /locus_tag="MBZ71_000833"
     CDS             6975..8744
                     /gene="aspS"
                     /locus_tag="MBZ71_000833"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBT4428804.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            8853..9728
                     /locus_tag="MBZ71_000834"
     CDS             8853..9728
                     /locus_tag="MBZ71_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4428805.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            9994..11877
                     /locus_tag="MBZ71_000835"
     CDS             9994..11877
                     /locus_tag="MBZ71_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBT4428806.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(11952..12236)
                     /locus_tag="MBZ71_000836"
     CDS             complement(11952..12236)
                     /locus_tag="MBZ71_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBT4428807.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(12226..12960)
                     /locus_tag="MBZ71_000837"
     CDS             complement(12226..12960)
                     /locus_tag="MBZ71_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBT4428808.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            13089..13712
                     /locus_tag="MBZ71_000838"
     CDS             13089..13712
                     /locus_tag="MBZ71_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBT4428809.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            13904..15025
                     /locus_tag="MBZ71_000839"
     CDS             13904..15025
                     /locus_tag="MBZ71_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBT4428810.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            15032..18160
                     /locus_tag="MBZ71_000840"
     CDS             15032..18160
                     /locus_tag="MBZ71_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBT4428811.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            18179..21373
                     /locus_tag="MBZ71_000841"
     CDS             18179..21373
                     /locus_tag="MBZ71_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBT4428812.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILPLYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(21519..22226)
                     /locus_tag="MBZ71_000842"
     CDS             complement(21519..22226)
                     /locus_tag="MBZ71_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBT4428813.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         7631 a         3998 c         4797 g         6215 t
ORIGIN      
        1 aaatcgtttg aaaaagcttt taaaaagcga acatttccgt aaaaaaacgt ataactgcac
       61 ggattttggg acaattatca aacagatatt tgtcccgatt cccgttttac cgaaaaaaga
      121 aagggggatc gattcctgtg acaaaagaag aaattttaac gggacccggt gtcataaagc
      181 tcgttagtca atacatgggt cccgaacatg tagcatttgt tgagaaagca tgcgaatacg
      241 cgactgctgc acatgacgga cagtttagaa aatcaggcga accctacatt attcatccta
      301 tccaagtcgc aggtatatta gcagatttaa aaatggatcc ccatacagtg gctacaggct
      361 tcttacatga tgttgttgaa gatacagaaa tcactttaga agatctgaga gaagaatttg
      421 gcgatgacgt tgctatgtta gtggacggcg taaccaaatt agggaaaata aaatataaat
      481 cccacgaaga gcagctagca gaaaaccacc gaaagatgct gctagcaatg gctcaagatt
      541 tacgagttat catggtcaaa ttagctgata gactacataa catgcgtacg ttgaagcacc
      601 tgcgagaaga taagcaaaga aggatcgctc aggaaacttt agaaatctat gcgcctcttg
      661 cacatcgtct agggatcagc cggatcaaat gggaattaga agatacagca cttcgttatc
      721 taaatccaaa acagtattac cgtatcgtcc acttgatgca gacgaagaga gaagaacgtg
      781 aaaaatacgt aagtggtact gttgaagata ttcgaatagc gacagaagag ctggggattt
      841 ttgcagaaat ctatggacgg ccaaaacaca tttattcaat ctatcgtaaa atgaaagatc
      901 agaaaaagca gttcaacgaa atttatgact tgctagctat tcgagtgatc gtagattcaa
      961 tcaaggattg ttatgctgta ttaggagcaa tccacacaaa atggaaacca atgcccggca
     1021 gatttaagga ttatattgcc atgccaaaag ctaatatgta ccaatctttg catactaccg
     1081 tgatcggtcc ggcaggaaat ccggtagaaa ttcaaatccg aacacaggaa atgcatgaaa
     1141 tcgctgaatt cggggttgct gcacactggg cctataaaga aggaaaaaat gaaaaagtag
     1201 aaccagatgg tatgacgaaa caattaagct ggttccatga gatactcgaa cttcaagacg
     1261 aaagctatga tgcttctgaa tttatggaag gcgtaaaagg agatatcttt agtgataaag
     1321 tctacgtctt cacaccaaaa ggagacgtta ctgagttacc aaaaggatcc ggaccattgg
     1381 actttgcata cagtatccat acagatatcg gtaacaaaac cactggtgca aaagtaaatg
     1441 gcaaaatggt gcagcttgat tacaaattga aaaacggaga tatcattgag atcatgactt
     1501 ctccaaattc atttggccca agtcgcgact ggttgaaatt agttgctact agcaaagcaa
     1561 gaaataagat caaacgtttc ttcaaagccc aagatcgaga agaaaatgtg atcaaaggcc
     1621 acgaatccgt ggtcaaatgt attacagatc taggatttac gcctaaagat attttgacga
     1681 agaacaaact gcaagaagca ctcgatcgtt ttaattatca aacagaagat gatctctatg
     1741 cagctgtagg gtatggagaa gttagcccct tgacgatggc caatcgtctg actgaaaaag
     1801 aacgtaaaga acaaaaaatc gagcagcaaa agcaagaagc agaagaaatc atgaatcagc
     1861 cgaaaaaaga acctgacaaa atgaaagtac gtcatgaagg tggcgttgtc attcaagggg
     1921 tagaaaactt actaattcgt atcagtcgct gctgtaatcc gattcctggt gatgatatcg
     1981 ttggttatat cactaaaggc agagggatat ccattcatcg tcgagattgt ccgaatgttc
     2041 agcctgacaa accaaatgta gcagaacgtt tgattgaagt cgaatgggaa gatacatcga
     2101 atacacgaaa agagtatgat gcagatttgg aaatttacgg ctataatcgt tcaggcttat
     2161 tgaatgatgt acttcaaaca gtcaatgcgc taacgaaaaa tctcaacagc gttgaagcac
     2221 ggacgaataa agataaaatg gcgacgatcc atttgacggt tggtatccag aatttatccc
     2281 acctaaagag tatcgtggat aaaatcaaag cagtacctga tgtctacagt gtacgccgga
     2341 cgaatggata ggaggacaga tatgagagca gttgttcaac gggtaagtaa agctgctgtg
     2401 tcagtcgatc agcaagaaat tggaaaaatt ggctacggcc tacttgtttt attaggcatt
     2461 catgaaaccg atacgcaaaa agatgtggac tatctggtaa aaaaaattgc acaaatgcgt
     2521 atttttgaag atgaacaagg aaaaatgaat attagtgtag aagatgtggg aggacaaatt
     2581 ttaagtatct ctcaattcac actgtttgcg gatacgaaaa aaggaaatcg accaagcttt
     2641 gtggaagccg cacgaccaga aacagctatt cctttatacg aatcattcaa tgaaggaatc
     2701 agaagtcgcg gcatcacagt cgctaccgga caattcggag cagatatgtc ggtttctctc
     2761 gtcaatgatg gacctgtaac gatcattatt gattctaaaa acaaataaag taatctggtt
     2821 ttcactagta aaaactatga aacacgtcgc ttaagttaga ctgataaact ctaacttaag
     2881 tggctttttt gtctgcaaaa agcgcttctt ttgtcagcag tcggaaaaaa agttaagctt
     2941 aaattataca agttattttt tataggaggt tgctgaagat gaaagaagta ccagtagtag
     3001 atatcaaaat cacagattta aaaaaattgt atcaaaaagg atacaacatg ttggaggagt
     3061 tacgccatga agcggacgct cccgtcgtca aagcaaagat tttcaataaa gaagctatca
     3121 cgatctacgg atcatcagca gcaaaagtat tttatgatcc aaggaatttc aaacgaaaag
     3181 gtgctatgcc aaaattagta ttgaagacac tttttggtca aggtggagta cagactttgg
     3241 atggagctgc tcatcaccat cggaaaaata tttttatgga tctaatgaca ccggaaagaa
     3301 tggaagatta tcatcgtatc ttagataaga atctgacaca agcacttgaa gcacagcatg
     3361 gtcaatttga actatttgat ttaagtaaga tggttttctt tacatctatt tgcgagtggg
     3421 caggtatcaa tttatcagcg atttctaaag atgaggtcga aaaattagca gaatatcaaa
     3481 tctctatgat cagcggaacg tttacttctc cgattgatca cataaaaggt gtcgaaaatc
     3541 gcaaaaaatc agaaaaatgg gcgcaaggct tgattgaaga agctcgacaa aatcctgtgg
     3601 ctggaaaaga aaatgtggca ttatatgctt ttgccaatgc cacagattta gatggtcagc
     3661 ttttacctct ggaagtggca gctgttgaat tattgaacat catccgtcca acggttgctc
     3721 taactgtgtg ggcggcattg atgggacatg cacttttcag tagaccagat ttgtatcagc
     3781 aattaaaaaa tgacttttcg acgttacaag atccgtttat tcaagaaatg agacgctatt
     3841 atcctttctt cccaatgcta cctgcgattt cgctgaaaga agtagaagta gatggttatc
     3901 gtataccaga aggcagttgg gttattttag atttatatgg aacagaccat gatgaacgaa
     3961 cagttgaagc gccagattcc tttatgatca aacggtatgt agggaaagca aaagatattt
     4021 cctataaaga agaatatgag atgattgcac aaggcggggg aaattttcga caaatgcatc
     4081 gctgtgcagg agaatggatc actcttcata gtctacgtgt gttttccgat cagctagtaa
     4141 ataagtttga gttctctgta cctgaacaag attggacgat tccattcaat cagtttccga
     4201 cttatccgaa tagccgagcg ctactttaca agaattagat tagcataaac ttatcgaaaa
     4261 aaaggaagat caaacagtgc tgatcttcct tttttcatat tactataaat tgataaggtc
     4321 aattgatcaa aatccacgaa atgcttcatc aatggtttga taatcttcat tggataaatg
     4381 caaatctaaa gcttggacat tgtcagatac ttgctctgga cgcttagctc caggtataac
     4441 tacagacact cgtggattct tcatgtacca agctaaaaca agttgcgcag gagtggcttg
     4501 gtatctttta gcaatggggc gaagtacatc gacagcggtt aaaatttctc tgaaacgttc
     4561 tccttggaaa tctggatttc tttttcgagg atctccttca ccaaattgtt tttcctcacc
     4621 aagttcatat tttcctgtca gcaaaccgga agcaagagga aaatagggaa caaaactgat
     4681 tttgtttttt tcaagataag ggaataattc tttttcagcc tgccgatgga tcaaactgta
     4741 tttatcttct actacatcga catatccatc tgcattcgct tctttcaatt gttctaacgt
     4801 gaagtttgac acaccgactg ctcggatttt tcctgcttct tttaattcgt gcaatgtagc
     4861 aactgattca ttttttggtg tgctttcatc cggaaagtgg atataaaaaa tgtcgatgta
     4921 atcagtttgc agacgtttta gtgcatcttc caccgattgt ttgagaaact caggagaatt
     4981 atcaaatgtt cgccctttat ttggtacatg agcagctttt gtagcgatga cgacacgtga
     5041 gcgatcatat tctttttcct tcagtacctc gccaatcagt tcttcggaac ggccgtttcc
     5101 gtaagcataa gcggtatcga tcaaattgat cccgctattc aaagctgtac gaactacttg
     5161 ttttcctgtc tcatcttcca aaccgtcaaa aagattatgt cctccaacgg catttgctcc
     5221 taatccaagc tgttctgcgt agacttgtga atgtccgatt cttatttctg ccatagctga
     5281 ttcctccttt ttgtctagtc taaaacaaaa taccaaaaag ctgaactaaa acgtttttgt
     5341 gaggggaaat agtcagatat ctcttgactt tgagactggt ttttagtagg ttataagtaa
     5401 tatcgaatgt caatgaaaga gcgagtaagt ttttgaaact ttacaagaga ggatcatcga
     5461 ggctgaaaat gatctaaagt ttcattagcc taaagacact ctggagatgg acgagtgaaa
     5521 agccgtcccg cgtacgagcg ttattcgtaa gaggaaagag aaatctttca aaattaggtg
     5581 gtaccgtgta tgaatttcat tcacccttgt tcatgatgaa caagggtgat ttttttattt
     5641 acatagctaa gaaagggaga gaaaactatg agtttccaaa gaccaaaagg aacaaatgat
     5701 attttgccag gcgaatcaga aaaatggcag tttgtcgaag aaacagcacg tttattattc
     5761 agagattatc agtataatga aatgcgtaca ccgatttttg aacactatga agtgatatcc
     5821 cgaagtgtag gggatacaac ggatatcgtt tcaaaagaga tgtatgattt ttatgacaaa
     5881 ggggaacgcc atgtgacgct tcgaccagaa ggtacagcac caatcgttcg ttcttttgtt
     5941 gagcataaat tgttcggccc agagtatgcg aaaccgttca aaacttttta tatgggacca
     6001 atgtttcgct acgaacgccc tcaaaaagga cggttacgtc aattccatca aatcggagta
     6061 gaagcatttg gatcagaaaa ccctgcaact gatgtggaga gcatggtgat ggcacttgat
     6121 ttcttcaaac agttggggat cacgcaaatc cggctagtca tcaattcatt aggtgataaa
     6181 gaaacgcgtc aagcctatcg tcaagcattg atcgactatc ttttaccttt tgaatcagag
     6241 ctgagtgaag actctaaacg ccgccttcat gaaaatccat tacgcgtatt ggacagtaaa
     6301 gacaaacgag atcaaaaatt tgtggcagaa gctccatcta tcttagatta tctaagcgag
     6361 cctgctcaaa aacattttga taccgttgtt tccatgctgg atgcattggc tgttccttat
     6421 gaaatcgaca gtaatatggt tcgcggacta gattattaca cacatacgat ttttgaaatc
     6481 atgagtgacg caccaaaaat gggggcacaa gcaacgattt gcgcaggcgg acgttatgac
     6541 aatctagtgg aagaactagg tggtccttca acacctggct tcggatttgc aatgggtatc
     6601 gagcgattac tcttgactat ggaggcagag gaagtagtta tccctgcatt taatgagttg
     6661 gatgcttacg tggtcgcact cggagacgaa acaaatatcg aagcattgaa agtggtccaa
     6721 gcgattcgaa actttggttt ttcagctgac cgcgacttca tgaaccgtaa agcaaaagcg
     6781 caatttaaga cagcagataa agcgaatgcc aaattagtat tggtgatcgg tgaagatgag
     6841 cttgctagtc aaacgattaa tgtcaaagca atggcaagcc gaaaagaaaa aagttttcca
     6901 ttagaagaga tatatgaaca ttttgatgaa gtctatgatg aaatgacttt attcgagtag
     6961 gaggaaaaaa ataaatggca caaagaacga tctattgcgg attagtttcc gaacaacaat
     7021 tagaacaaac agttacatta aaaggatggg tacaaaaaag acgagacctt gggggagtaa
     7081 tctttatcga cctacgtgat cgtgaaggaa tcgttcaaat cgtctttaat cctgaaatca
     7141 acaaagaagc atgggaaatc gcggataaat gtcgcagtga atacgtgatc gaagtgactg
     7201 gggaagttag aagacgcgga gaaaatgcag taaatcctaa aatggccact ggtgaattcg
     7261 aagtgattgc aagtgaaatc gtgattttga atcaagcaaa aaccccgcca tttcaaattg
     7321 aagatgatca agcaatcagt gacgaaatac gtttgaaata tcgttatttg gatctgcgtc
     7381 gtccaaaaat gactaaaaac atcaaattaa gaaatgcgac aacaaaagcg attcgtcatt
     7441 atttagatga taatgacttt ctcgatatcg aaacacctta cctagggaaa tctacaccag
     7501 aaggcgcaag agactaccta gttccttcac gtgtccatgc tggacatttc tatgcgttgc
     7561 cgcaatctcc tcaattgttt aaacaattgc tgatgaatgc tggattcgac cgttattatc
     7621 aaatcgttcg ttgtttccgc gatgaagatt tacgtggaga ccgtcagcct gaatttacgc
     7681 aagtagatat tgaaacaacg ttcttaacag cagaagaaat ccaaacttac acagagggat
     7741 tgattgcgaa ggtaatgaaa gaagttcgcg gtatcgaagt cacattgcca tttcctcgta
     7801 tgacctatga tgaagcaatg gctcgctacg gcagcgacaa accggataca cgttttgata
     7861 tggaattgat cgatctaagc gatacagtca aagaagtaga attcaaagtc ttccaaatgg
     7921 ctcttgaaaa cggcggtgtc gtgaaagcat tgaatgctaa aggcgctgca gatcgctact
     7981 cacgaaaaga catggatcag ttaggtcaat atgtaggaca attcggagct aaggggcttg
     8041 catggctgaa agtagaagaa gacggattga aaggtccaat tgctaaattc atgggagaag
     8101 caacagaagc aatcattaaa gcaacagatg caaaaccagg tgacttgctg atgtttggcg
     8161 cagataaaag tgaaatcgtc gctgctgcat taggtgctat ccggacacgt ttaggaaaag
     8221 aattaggact aattgatgaa tcgaaattca atttcttatg ggtaactgat tggccacaat
     8281 ttgaattttc agaagaagaa ggtcgatatg tctctgctca ccatccattc acgatgccaa
     8341 aagaagaaga tatcccgtta ttggctgaag accctgcaaa agtctatgca gaagcttatg
     8401 atatcgtctt gaacggttat gaactaggcg gaggttctat ccgtattcat actcgtgagc
     8461 ttcaagaaaa agtactagaa actctaggct ttacaaaaga acaaatggaa gaacaatttg
     8521 gtttcttgtt aacagcttta gattacggat tccctccaca tggcggtatt gcattaggat
     8581 tggaccgttt tgcaatgtta ttagccggag aagataatat tcgtgaagtc attgcctttc
     8641 ctaaaaacgg aaaagcagca gatgtgatga gtgatgcacc aagtatggtt tcgcctttac
     8701 aattgttcga attgaatatt gatgtaacga atatagaaga atagccgata aaaaggtaaa
     8761 aaggatttgt cgaatacgct tcttttgaca accttgattt aattgaaaaa cactttataa
     8821 tgaagccata acatataaag cggggctttt ccatgaaaaa aacattcgaa aattgggctt
     8881 atcatgatta cacgaccaag ctttcggtat ctatcgttta tgcgttgatc gcatcagtcg
     8941 cattgaattt cttttatcag ccaggacata tttattcaag tggtattact ggtctagctc
     9001 agattttgac gactttgtca aaatcactga taggattcga agtaccggta tcaacaactt
     9061 tgtatttact gaatatacct ttgtttgtat tggcgtggtt aaaaatcggg aagaagttta
     9121 caattttcac ttttatcaca gtcactttga cctccctttg tatccatatt ttaccggaaa
     9181 cagtcctatc taccgatcca atcatctgtg cgttgtttgg gggggctgtg atgggactag
     9241 gtattgggct agcgttgaga aacggcctat catcgggcgg tctagatatc gtgagtatca
     9301 cgattcgtaa aaaaacagga aagtcgattg gttctatttc gatctacttc aatgcattga
     9361 tcgtttttgt tgccggaatt ctatttggct ggcaatatat gttttacagt acgttgtcta
     9421 ttttcgtcag tggaaaagta actgatgtcg tttatactaa acaaaagaag atgcaggtaa
     9481 tgatcgtaac gaaacatcca gaaaaagtag taaatgaaat ccaaaactgt atgcgtagag
     9541 ggatcacgat catcaatgaa gcagaaggtg cttataagca tgataagcag acagtattga
     9601 tgacgatcgt tacacgatac gaactgcctc tacttagaga agcaatgaaa gcttctgatc
     9661 cacaagcttt tgtaagtatt gcagaaaacg tccagatact tggtcgtttc tatgaggatg
     9721 gcatttagat cgagtttgac aaaaattcat gaaatactgc tgtagacaga cgttcgtttg
     9781 tctgcagttt ttttctacca tagtagaatc agcaaagaaa aactgctttt ttatttgaaa
     9841 ttttcttaaa gttgaaaaaa agcatgttta tttttttaaa tttatgggat gatagaaaaa
     9901 aatagtagag aaaggacacg attctacttg ctattttcac ttaatacccg tatattacga
     9961 atgaaaaaag tttatgaaaa gttggaatat tgaatggaaa aacaaaaaag gttaaaaaac
    10021 agccgctaca tcactgggtt tgatgggctc cgttcattag ctgtggtggg tgttatcttc
    10081 tatcaccttc tgccaactag tatgaaaggc ggctatttgg gcgttccgat ttttttcgtc
    10141 gtatctggtt atttgatcac ggatctgctg cggcaggaat gggaacaaaa tggaaaaatc
    10201 aatatttggc aattttatgt cagaagaatg aagagactct atccggcact tgtttttatg
    10261 cttgtgacag cttcggctta tatcactttg tttcagcgag ggctgttaaa caatcttcga
    10321 ggagttgtgg tcagcagctt actgtatgtc aacaactggt ggcaaatcaa taacggttta
    10381 tcttatttcg atcgatttgc taatgagtca ccgtttacgc atatctggtc tttagcagta
    10441 gaaggtcaaa actatttggt ttggccgatc ttgtttgtat tgttgatggt atttgttcgt
    10501 aaaagaaaat ggatctttta tactgtgtta ggtacatcct tcctctccgc catcttgatg
    10561 atgatcttgt atacaccagg aggcgatcca acccgtgttt attacggtac cgacactcgt
    10621 ctgttttcta tatggttggg aagtgcgctt gcgtttgttt ggccaagtac gcgtttaaag
    10681 aaaaacatcc cgaaacaagc gaaaaatatt ctgaatatcg ctggaggaat ctcgcttttg
    10741 gcactgattc tttgcttctt ctttttagat gatcattaca atttcatcta ttacggagga
    10801 atgctcctag tgagtctgtt atgcgtagtg ctagttgcag tgacagcaca ccccggagca
    10861 agtttgaatc gatggctgac aaatccagta tttacttgga ttggcaaacg aagctatggc
    10921 atttatctgt atcagtttcc agttatgatt ttttatgaag caaaggtgac tgatattgcc
    10981 gatcatgttt tgttgcatac tttagtggaa atcacattga ttctaatcat cagtgaactt
    11041 tcttatcgat atgtggagcg tccgctagca cgttttgatt atggtcgtac attccaagtg
    11101 atgaaaggat ggttcagcaa accgatcatc agtaagagca aaccatgggt tgttccaggc
    11161 acattacttg tagcaattgc actcgttggt ttcgttacgg caccgaaaaa cgctgtaaca
    11221 gccgatcaaa aacaactgca ggcaaaaatc gctgaaagta aaaaattagc agaagaaacc
    11281 agaaacaatt cagaaaacac cactagttcg gttgaccaag cgatccttga taagtacgag
    11341 ttgtccaagg cacaagggaa taaggcacaa aaattagaac taacggcatt tggtgattca
    11401 gttatgttgg atgctgcaac ggatctacaa gaactttatc caaaagtagt ggtagatgga
    11461 gatgtcggac gacaattata tgccagtgta ccgtacattg aaaaattaaa ggaacaagat
    11521 ttgttgaaag acactgttct gatcggacta ggaacgaatg gctcattctc ggaagctcag
    11581 tttgatagtg tgatggatgc aatcggtgat cgaaaagtat actgggtcaa tgtccgagta
    11641 ccgacgaagc gctggcaaaa tgacgtgaat gcgatgcttg aacagatgac gaaaaaatac
    11701 gacaatatga cgctgattga ttggtacagc tacagtaatg accatagtga ctggttttat
    11761 gatgatcaag tccatccaaa tcctgaaggg atgacacatt acattcactt agtcagcgaa
    11821 gccttgctag gaggaagttc aagtacgaac agcagtgaga caaacgtgac agaatgatag
    11881 ctacaaaaaa cttcaggcaa aaacatttgt ttttgcctga agttttttta tatttaccgt
    11941 gtttttaaca ttcatataga ttcaggaagc aaaacggttg agtgtgtctg taaataacgt
    12001 tctttttgtt ttcgggtgag ttttttgaat gcagcttctg cttttgttgc ttcgcttctt
    12061 gaagaaaata cttctgcatg gatcatctta acaggtcttc ttttttgaag acgagtatat
    12121 ttagcaccag taccattgtt atgttcctct aatcgtctag aaagatcggt tgtatatcca
    12181 ccataaaatg tctgatcttt acagaataat acgtaaaaat aatgttcatt tgccatagag
    12241 catctcacga atctctgcag tgtattctcc atccatattg taagtgatca atggtggagc
    12301 gactttgaat ccgtctaatt tgccttcttt gattccttca actaacaaaa tatttgcctc
    12361 ttttccaggt tttgggtaaa caaattggat ccttttaggt gcaattcgat agtcttccat
    12421 gacctgaatg atttctaaga accgatcagg acgatggacc atagtaaagc gtccatttgt
    12481 ttttagtagc ttacttgaaa tatcgatcac ttcttctaac gtcgtatgaa tctcatgacg
    12541 ggcgattgcc aggtgtttat tagggttcgt tttatttgtt ggcagccctt tgaaataagg
    12601 tggattacag acgactaagt cacaagaatt atgtcgaacg acagataagc tgtctttcag
    12661 atcgatggtg tggacagtca tttgttcatt taaatggtta agcgcgatac tacgtcttgc
    12721 catgtctgct agacgaggtt gaagttcgat agcgtcgata gccgcctggg ttcgtttact
    12781 taaaaaaagt ccaactgcgc catttcctgc acaaaggtca acaatcttcc cttttttagg
    12841 tacacgggaa aagtgagcaa gcagcacagc atctaaagaa aaagaaaaca ctttggaact
    12901 ttggataatc ttgatatctt ctgcgtataa ttggtcgatt cgttcgtctt cgtgaagcat
    12961 ggattccctc caaaatgaaa aaaatagtcg tctgtattat actattttta ttaaaaagaa
    13021 ccaaggtggc ttgtcaaacc tctatttttt atgcatacta tgaccataag aagggaaagg
    13081 aagagactat gttttatcga tttatgcgcg gggtagttcg agtactatta gccttagtta
    13141 atggaaatgc ccgctatgag aataaagaag ttttaccgca agatgagaat tacatactag
    13201 ttgctcccca tcgtacttgg tgggacccat tatatctggc ggtagctgca gcacccaagg
    13261 aattcagctt tatggcaaaa gaagaactgt tcaaaaatcc agttctgcga tttatattaa
    13321 ctcgatccaa tgcttttcca gttaaaagag aaaatccagg accaagcgtc attaaaacac
    13381 ctgtaaagat tttgaaatcg acaaatttga gtttgatcat gtttcccagt gggacaagac
    13441 atgctacaga acttaaaggc ggaatggcat tgatttccag aatgtctaaa gcgaaaatcg
    13501 tgccagctgt atatcaaggt ccgttgacac ttggggatct gttcaagcgt cagagagtga
    13561 cagtgcgttt cggcgaacca atcgacttat cagatatcaa gaaaacggat aaagaaggta
    13621 tggcggagat cgaacgacga acacaagcag cttttgacaa attggacaaa gaagtgaacc
    13681 ctgatttcaa atatgaagtc aaaaaaaaat aaaatgataa gtttatgcgt gggcggtctt
    13741 ttgaatctac tgtcctgttc agttcagact ttttcaatga aaacgaggga gagacgtcaa
    13801 gtctgccctc tttttgcgtt ccaatatgta aaatagaaga caggtgtcat gtaatggatc
    13861 aaagtgttat aataattaga aggacatttt ttgaggtgat tgaatgcgtt ttttacatac
    13921 agccgattgg catatcggaa aaaaactaca aggttatgac ttgattgaag accagcgtca
    13981 tgtactatct tctatactag atatcgcgaa agaagaaaaa gtagaggcaa tcgtgattgc
    14041 cggagactta tatgatagga gtgtcccagc tgttgatgcc gtagaattat acaatgaatt
    14101 gatggtcgat tggaatttaa aggaaaagat cccgattttt gctatatcag gaaatcacga
    14161 tagcagtacg agactttcag cgggtacgcc atggttcagt tattcgaact attttttgcg
    14221 aacacagcta gcagaagcat ttgaaccagt cgagtttaaa aacgtacaat ttttctttct
    14281 tccttacttt gaaccaattg cagcgagatt atattttgaa gatgacaata tccgaacgat
    14341 caagcaagcg gtagccaagg tgatagataa aatgcaggag ctatttgatt cagaaaaaaa
    14401 acaagtattg attagccatt tttttgtaat gggaagtgta aaatctgatt cggaaacgaa
    14461 aatcgaagta ggcgggttgg attcgatccc tggtgaacta cttacaccgt ttgattatgt
    14521 agcactagga catttgcata atcaagctgc tttgagacag gataatgctc gctatagcgg
    14581 ttccccattg aaattctctt tatctgagat caaccaaaaa aaaggcgtat ggatcgttga
    14641 cctgacaaca ggcctggagt tgaattttag ggagatcgaa cccctgcatg atattgaaga
    14701 aataacagcg agtttcaaag aattgctgga tcctgccttt tatcagtccg tcaatcggga
    14761 gaattacatc tatatctatc tcactgatag aacgatcatc ccgaatatga tgaaccagct
    14821 tcggcaaata tatcctcgta ttttggggat tgaacgggca aacggtcgag cagaacaaac
    14881 aaagcgcaaa cgaaaagtac tagaaatgaa agatcctcaa aaattagcag aagcattttt
    14941 tagtgaaatg acggaagagc agctaactgc aaaccaagaa aaatggctcg gagaaacgtt
    15001 gcaagaaatc atggaaatgg aatgatcaag aatgaaaccg aaaaaattac aacttaaaaa
    15061 ttttggtcct tttatcaatg agaccgtaga tttttcacgt ttgacagaag caccgctttt
    15121 tttgatcagc gggaagacag gagctggcaa aacgacgatt tttgatggga tcaccttcgc
    15181 actttttggt gaaacttccg gaagacttcg ctctggaaaa gagatgcgtt ctttgtttgc
    15241 tacaccagac gaagaaacaa gcgtgacttt ttcttttgaa catcagcaga tgagctatga
    15301 aatcgaacga aaaccagaac aagttttagc aaaacgaaaa ggaaacggaa caaaaaaaca
    15361 ggcagccaaa gtaacgttga ccatttttga tgggaaagga aaagaaataa aacaaatcac
    15421 aaaacgtaca gaagcagatc agttgatcag agaactgatg aatctcgatg cgaaacagtt
    15481 ttctcaaatc gttttactac cccaaggaga attccgtaat tttttggttt catcaagcag
    15541 tgataaagag actgttttac gtcatttatt cggtactcaa tttttccaac agttcaatga
    15601 gcgattaaaa gaaaaagcaa aacaacagca acaaaaattg gatcatttag agcaagagct
    15661 gcatcttctg caaaaaaggt ttgttttagt caaagaagag gaaccagcag cagggtttga
    15721 agccgtttta gcccaatgga caaaccatca ggcaatactc gaaaaacaaa tcacggaaga
    15781 aaaagaagct ctaacggaac ttcaggaaaa acaaaagcaa ctagaaaatt cctactatcg
    15841 ttttgaaagc ttgaaaaaaa gttatgaaga aaaaacgata ttaataaaca agcaaaagga
    15901 attagctgct caaaaagacg aaatagagga aaagaaacga tggattgggt attatgaatt
    15961 tacgcaacga ttagtcgaac caatccagcg cctacaagaa ataaaagttg aaaaaataga
    16021 actgtcacaa aaaagtagtg agcaaacaaa gaaactagcg atgactacgc agaattatga
    16081 agagtggcaa aaaaatgaag caaaccgcgt acgccgacaa acaaaaatca aagaagctgc
    16141 tcaaaaactg caaaacaaac agatgatggt gccaattgtc gaatccttac gtagtaaaaa
    16201 acaagacgca gaacaattaa caatttcatt ggataaagaa gaattgaatg ccgagaagct
    16261 gtatcaaaat ctcgctatct atcaggaaag aactcgaatt cttcaagaaa atttactgga
    16321 tcaggaaaaa ctgcaagaag atcgtcttgc ttttggacag ttaaaatatc tagatgaaaa
    16381 gcagcaagaa atagaaaaac aaaaagcaaa agacactggg tcgctcgcac agataagaga
    16441 agaactcaaa aaagcaactg aagaaataac aaaaaatagt cagctattga aagaacagac
    16501 agaacaagca gaacagctga aaagccaatg ggcaaagcag caaattgctc gtcttcagct
    16561 ttttttgcgt ccaggggagc catgtcttgt ttgtggatca acggaacacc ccatacaaca
    16621 attaaaacac gaacagccaa ataaggaaga tatcttaaaa gtagaacaac aactggcaga
    16681 agctgagtct gctgtagaaa gaactcagca aatcattgcc aaaagtgaag cacatgaaaa
    16741 taatcttcaa caacaattat cagatttaga acaaaaaatc atggcaacga atatacaatt
    16801 ggctgaacaa gaagaacact tgcaacaggc tttgaaaaag gcatttcctg agataacaga
    16861 acaaaaaaca aaaacaactc ttgtagtaat cgaacaatct ttagatagac gaaaaagcaa
    16921 gatggccaat gcaagaatag agctggaaga aataagacaa caagcagaaa agctcgaaca
    16981 acaattagct gtctacaaac agcagcagac agaaaaaaat aatcagctcc agcaactgca
    17041 aggagaaatc aaaagtttag aacaacaatt gcaagatacg gatagtaact gtttagaaga
    17101 ggagatacga cgacttgaaa aacagttaga agaagactcg aatcaactag ctgttgagca
    17161 gaaagcagga gtgcaattgt tgcaagatct tcatacgctt cggacacagc ttgatcttca
    17221 acatgaacaa atagaaaaaa tagatgaaaa aatagaaaag gaacaagaaa gaatagctaa
    17281 aaagttagaa agccagtcgt atttttcaac aattgaagaa atctgtgcat ttgcacaaca
    17341 gcaagcacaa tataaagaaa accaacgtac agttacttct tatcatgaag agcaattagt
    17401 gacagctgac cggctgcgtc aattagaaaa tatccagcta actgagacat tcccagatac
    17461 agaagagatc agccaacagt tagaagaaat aagacgtatg atcgaaataa agcaagaaaa
    17521 gatatacggt ttacaagaac aaaaaattag taatcaaaaa atctacgatg aatgtctttc
    17581 gatttatcac acaagccaga cgaaattaga tgagcttacg caattacagc agctttcgca
    17641 aacattcaac ggagaaaatc ctaagaagac tagtttggaa cggtatgtgc ttcaggttta
    17701 tcttcaagaa gtgttacaag tagcaaatga tcatctccaa cgtttaacga aaaatcgtta
    17761 tcaatttgag ctagcggata ccattggcag ttatcgaggg aaaaccggat tagaaatcaa
    17821 tatctatgat gatcaggcag gtatgtctcg aagtgcgcat accctgtctg gcggtgagag
    17881 ttttattgca gcactttctt tagcattagc tcttgccgat gtgatccaga cacaagctgg
    17941 tggtgtagca atcgatgcat tgtttattga tgaaggattt gggtcattag atgaagaagc
    18001 tttggaaatg gcaatggaag ctttagaaac gatcgaaagt gaaggaagaa tgatcggcat
    18061 cattagtcat gtaagagaat tgaaagaacg agtattacaa caaataagaa ttgatacaga
    18121 aggtagcgga caaagcaaaa tacgctatca acttggataa aaagggagat tggtaactat
    18181 gaaatggagt attccagaaa aagtcattgc acgtggacgt caatacttgc aagaaggccg
    18241 agtgttatcg gttgtccctg accaagaaga aaacgtgtgg catgcagaag tcttgggcag
    18301 tgaattgtat atggttgatt tggatggtac agcaaaagag atagattact gccaatgtcc
    18361 ctattgggag gaacataaat attgtaagca tacagttgct gtggaacttt atttacggtc
    18421 gaaaaatcta tctcgtatca tgaaaaaaga ccagccaatg ctagaaccgg taaaagcaac
    18481 aagtgaggga gaaatcattg caaaaggttt ttctcgtcta aaaacacaaa acaatcctca
    18541 agcggtgcaa cctttgatta ttgaatacca agtagaaacg attgagacta atcagtatca
    18601 cccagagctt tctattttgc cactttactt gcgtatcggt tcattggcat caccgaaaaa
    18661 aacatatatc gtaaaaaaca tctatgattt tttacaagct tacacagaaa aagaaactta
    18721 cactgttaat aaacagtacc agtttcgtct agataaacgg gcgtttcaaa aagaagatca
    18781 aaaaatactc acgcatcttg caggaagtgc tcagacgcat caattattag gtacgaacgg
    18841 cttacaagtc aaaggaaaac tagataaaag atatctgctt ctcccagtag aacaagcccg
    18901 cttcctgtta gagcagatga atcagactac acgtttgcat ctgcagataa atgaacaaaa
    18961 cgatcaagga atacaatttt ccgatcaaaa caaaccttta tcattcaaag tggaaaaatt
    19021 tggagaagat tatttgctgc aagcattgaa tgattttgat tttttcttta tgcattatca
    19081 gtggggagtt atccaaggga cattttattc tctgacgaaa tatcaacaaa cggtttatca
    19141 aacttggaaa caattgattc gccgattgga gaaaccagaa gtagtgttca agaaaaaaga
    19201 attaccgtct ttgtttaaag agattatccc tctgctgtca gagatcggcg acgtagctat
    19261 tgcccctgca gtagaagagg aagtagcaga tatccccgtc gaatttattt tctttttccg
    19321 aaaagcaaaa ggaaaaatcc aagctcgagt cgatttcgtc tatggcgaag tcatctattc
    19381 gactgacccg aagcatgagg tctcgacaga acaggctttt caggtgttac gtgacacagt
    19441 agaagagaaa cgagtacttg atctattcag aatgtatcgc taccagaaaa atgaaactgg
    19501 ttatgaacgt ccattgccaa gcggggaaga actatatgcc tttttccgca cagaactatc
    19561 tgtcttccgt cagtttggtg aagtcagact tggaagaaaa ttgcgagagt tgtatttaga
    19621 tgcgcataaa catcaaccga aaatcgaagt aaccgagacc ggttcatggc tcgatattcg
    19681 attcgatgtg acagggatag aggagcaaga aatcgatcat gtcctgcaaa gcttgctgag
    19741 aaatgatgct ttttatacat tagaaaatgg tcaggttctt tcttttgatt cagaagaatt
    19801 tcaacaaaca agccaagtgt tgcaacaatt acgggaatcg atacgtacag aagaaggaac
    19861 gatccatgta cctaaaaatc aaggattgat cattcaaaat caattggaaa aaagcaatgc
    19921 tacttttagt gaatcgttcc aaacgatggt acaagatctc attcatccag aaagatacca
    19981 ggcacagctt cccaaaggat tgaatgcaac gatgagagat tatcaaaagc aaggttttcg
    20041 atggttgaag atgttaggtc attatcagtt tggcggtatt ttagccgatg aaatgggact
    20101 tgggaaaaca ctacagacaa ttgccttttt actttcggaa aaagaagaac ggaaaagctt
    20161 ctctgctttg atcgtagcgc ctgcgagctt aatttacaac tggcaggcag aggtgagaaa
    20221 attcgcccct tctttaagta tccaagtaat caatgggaat aaaaaagaac gtgaagaatt
    20281 actggctaaa gacacagata tccgagtgac ttcttatgcc agcttaagac aggatctagc
    20341 taattatcag tcgcaaaaaa ttgattattt gattcttgat gaggcacaaa tggtcaaaaa
    20401 tagcagtacg aaaactgcac aagcattgag agaattagct gtacctcagc ggtttgcact
    20461 gagtgggaca ccaatcgaga ataatttaga agaattgtgg tcattgtttg caacgattat
    20521 gccaggattt ttcccgacta aaacaaaatt cagggaactt gccgcagaag aaattgcgca
    20581 aatgattcga ccgtttattt tacggcgaga taaacaaact gtgttgaaag acttgccgga
    20641 aaaaacagag atgaatctat attctgcact tacagaagaa caaaaaactg tttatttagc
    20701 ttacttgcgt caaatgcgtg aagaaatcac atcaatggat tctgaagcat tcaagaaaaa
    20761 ccggatcggg atacttgctg gtctgacaag actgcgacaa atttgctgcg atcctcgtct
    20821 gtttattgaa gattaccaag gcggttccgg caagctcgaa caagtaaaag atctactggt
    20881 agctgccaaa gagaacaaac gaagagtttt gctgttttct caatttacag ggatgctgac
    20941 tatcttgcaa gaagagttag ccgagctagg gatctcgaca ttttatttaa gaggcagtac
    21001 gaaaccacaa gatcggttaa gtatggtaga cgcatttaat gctggcgaaa aagatgtttt
    21061 tctgatttcc ttaaaggctg ggggaactgg attgaatctc acgggagcag atacggtcat
    21121 cttgtatgac ctttggtgga atcctgccat tgaagaacaa gctgctggac gtgcacatcg
    21181 gatcggacaa aaaaatgtgg tagaagtgtg gcgtatgatc tctgaaggaa cgattgagga
    21241 acgcatggat tcactccagc aagaaaaacg cgaactgttc caaaaagtga tccaaggtaa
    21301 tgaagaacag ttgacgaaac tgactgaaga agacatccgc atgattttaa gtgtaggaga
    21361 aatcgaggag taagagactc gaaaaagcgt tcagtaatgg gtaagaaagc ctttctaaag
    21421 aagcgattgt ttatttgatt taaaaaatag caaggaggct gggattccat tttatcccag
    21481 cttccttgct atcatttttt tatttctatt tttaaaggtt atacaaagat tgtcttcatt
    21541 gtctctaaca gctgatcaaa agaagaaatc tcgatatcgc aaattggagt ctgttcaatt
    21601 ttgcgttctc gatggttgaa ccaaagcgct tgccagttag cactttttgc acctagaaca
    21661 tcattgtcgt aattatcgcc aacgtataag gtctcttctg gaagcatatg aaattctttc
    21721 tcagctagct gaaaaatctc tttttctggt ttttggaaac ctgttgcttg agaaatgagc
    21781 atattgcggg aaggaatcca attgttcaat tgaagttgct tcagtttttt tgtctgatgg
    21841 tcagtcggcc catttgtgat aattcctaat gggacattct tttccttcaa gaaatcgagg
    21901 gtttttttga cctcctcatg caagcagata ttatcaagtt cttcctcgta aattttttga
    21961 aataagaggc catcttcttc cgtaatatga gggtagtcta aatcttttag tgattggctg
    22021 atccgatgtg ctcgcatata ttctagtgtc caatctcctg ccattacttt tgggaagttt
    22081 tcatcactat gatggcggaa ccggatatat agtggatgca tatctgcatc tgaaacaagc
    22141 ggaacgactc ttttgaccgc gtttcgaaat ggctgctgct gatcatacat cgtatcatca
    22201 acatcaaaca caactgattt aatcaaaatt ttacacgcct ttcggttttc attgattttt
    22261 tcacaaatcc ttgactattg taacagaaaa aattgtctaa aaaagctgat aaataaaggt
    22321 ttttcttact atatttctat ttccttgaca gaataaggtt tttatatttc agtttttttc
    22381 actaaaaatc gtgaaacacg tcaataagtt tgatgaacaa attaatcatt atggtagaag
    22441 tcgattgatt tattttaatg caatctctgt ggaattttcg aaagatttat ttttttaata
    22501 cagtctcaat ataactattc aaacataaaa taacgttatt tgatttattt gtttatagta
    22561 aattttattt agtaatcatt gctttaaaat actacgctta tataacagag aaaataagcg
    22621 ttataataaa gaacagtatt a
//
LOCUS       DAGCWU010000027        21903 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.027, whole genome shotgun sequence.
ACCESSION   DAGCWU010000027 DAGCWU010000000
VERSION     DAGCWU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.027"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1415
                     /locus_tag="MBZ71_000843"
     CDS             <1..1415
                     /locus_tag="MBZ71_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4428814.1"
                     /translation="LLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMV
                     IAAALVYPTIVSAYSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLH
                     ESIRNLLTPFICIIVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFW
                     QVLVIFGLHWGLVPVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSL
                     SLSAFITALFGITEPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSIL
                     SFPIYIGEGFVWLVASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDL
                     SEVNDPTFASGSLGEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHI
                     GIDTVNLNGKYFQSAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNI
                     ITSHKKNNQTRKLASLGGSI"
     gene            1412..2869
                     /locus_tag="MBZ71_000844"
     CDS             1412..2869
                     /locus_tag="MBZ71_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4428815.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            3453..3887
                     /locus_tag="MBZ71_000845"
     CDS             3453..3887
                     /locus_tag="MBZ71_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4428816.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            3988..5079
                     /locus_tag="MBZ71_000846"
     CDS             3988..5079
                     /locus_tag="MBZ71_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBT4428817.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            5109..5786
                     /locus_tag="MBZ71_000847"
     CDS             5109..5786
                     /locus_tag="MBZ71_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBT4428818.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            6118..7413
                     /locus_tag="MBZ71_000848"
     CDS             6118..7413
                     /locus_tag="MBZ71_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4428819.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            7403..8863
                     /locus_tag="MBZ71_000849"
     CDS             7403..8863
                     /locus_tag="MBZ71_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4428820.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            8999..10495
                     /gene="glpK"
                     /locus_tag="MBZ71_000850"
     CDS             8999..10495
                     /gene="glpK"
                     /locus_tag="MBZ71_000850"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBT4428821.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            10509..12332
                     /gene="glpO"
                     /locus_tag="MBZ71_000851"
     CDS             10509..12332
                     /gene="glpO"
                     /locus_tag="MBZ71_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBT4428822.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            12333..13049
                     /locus_tag="MBZ71_000852"
     CDS             12333..13049
                     /locus_tag="MBZ71_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBT4428823.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            13355..15283
                     /locus_tag="MBZ71_000853"
     CDS             13355..15283
                     /locus_tag="MBZ71_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBT4428824.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            15409..15963
                     /locus_tag="MBZ71_000854"
     CDS             15409..15963
                     /locus_tag="MBZ71_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBT4428825.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16448..16762)
                     /locus_tag="MBZ71_000855"
     CDS             complement(16448..16762)
                     /locus_tag="MBZ71_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBT4428826.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(16776..17036)
                     /locus_tag="MBZ71_000856"
     CDS             complement(16776..17036)
                     /locus_tag="MBZ71_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBT4428827.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            17206..17520
                     /locus_tag="MBZ71_000857"
     CDS             17206..17520
                     /locus_tag="MBZ71_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBT4428828.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            17522..19177
                     /locus_tag="MBZ71_000858"
     CDS             17522..19177
                     /locus_tag="MBZ71_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4428829.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            19260..21395
                     /locus_tag="MBZ71_000859"
     CDS             19260..21395
                     /locus_tag="MBZ71_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4428830.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            21570..>21903
                     /locus_tag="MBZ71_000860"
     CDS             21570..>21903
                     /locus_tag="MBZ71_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428831.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
BASE COUNT         7291 a         3689 c         4772 g         6151 t
ORIGIN      
        1 atttattaac tactgaatca ggaacttata tcattttgaa tgcagcagct gatgcatttt
       61 tctatttctt gcctatattt ttggcatata ctgctgcaaa aaagttcaat acagatcgtt
      121 tcatcgctat ggttattgca gcagctctcg tttatcctac gattgtcagt gcatatagtg
      181 atagcataac cttgcgattt ctagggatgc ccgtgattct agcaagatat acaagtacgg
      241 ttattccagc aattttagct gtatgggttc tttcttatat tgagcctaag attcgcaaaa
      301 gcttgcacga aagtattcga aatttattga caccattcat ttgtatcatc gtgatggtcc
      361 caattacttt attagttgta ggtccaattg cagattatgc aagtcagctg attgcagctg
      421 gatatttagc tgtttataac tttagccctg ttttatctgg tgctgttatc ggcggatttt
      481 ggcaagtgtt agtgattttt gggttacatt ggggacttgt accagtaatg accaacaatc
      541 tttctttcta cggtcgtgat acgctaggtc cagcatgtat gactgccgta gctgcgcaag
      601 caggagcagt acttggggta tttttgaaaa cgaaaaataa gaaagtaaaa agtttatctt
      661 tatctgcatt tatcacagca ttatttggaa tcactgaacc tgctgtttat ggtgtgactc
      721 ttaaatataa acgaccattt tacattgctt gtatatgtgg agctatattt ggtggagtag
      781 ctggtgccgc aggagccggt gctttggcag ttgctacaag aagtattttg tctttcccga
      841 tttatattgg tgaaggattt gtttggctag ttgctagtta tttcttagcg atgatcagtt
      901 cctgtatgct aacttttctt ttcggctaca aggatgaaat cgaagaagaa tcctcaaagg
      961 atatagtgtt aagtactcct gcagcaggtg aaataattga tttatctgaa gtaaacgatc
     1021 caacatttgc tagtggatct ttaggagaag ggtttgcgat cattccaacg gacggaaaaa
     1081 tctacagtcc tgtaaacggg gaagtttcaa cagtttttcc aacgaaacat gcaattgggg
     1141 tagtcagtga agaaggagca gagatactga tccatatcgg tattgataca gtgaatttaa
     1201 atggcaaata ctttcaaagt gcagtatctg atggaaaaaa agtacgtaaa ggcgatttat
     1261 tgatggaagt agatttgcaa gaattaatca aagagggata tgatccgacc acaatggtta
     1321 ttgttacaaa tagtagtaga ttcaaaaata tcattactag tcataagaaa aataatcaaa
     1381 caagaaaact agcaagttta gggggatcga tatgaaaaaa tttccagaag gatttttatg
     1441 gggcggagcg acagcggcca atcaatgtga aggtgcatgg gatgtagatg aaaaaggatt
     1501 atctgtgtct gatgtgtata cattcgacag cgatttacct aaagaaaaat ggacagatca
     1561 atggcatatg atgacgcatc agcaagtaaa agcggcgcag gatcccaaca gcaagtatta
     1621 ttatcctaaa agacacggaa atgatttcta tcatcacttt aaagaagaca tccgtttatt
     1681 tgctgaaatg ggttttaaat gctatcggat gtctatcgca tggacaagaa tttttcctca
     1741 tggtgatgaa gaaacaccca atgaagcggg attatttttt tatgatcaag tgtttgacga
     1801 atgtctaaaa tatgggattg agccagtcgt ttctctttcc cattatgaaa tgccgttata
     1861 tttggttaca gaatatggtg gatggcctaa tcgaaagctt attcagtttt acgttcgttt
     1921 cgctgaaaca gtattcaaac gctataaaaa taaagtaaaa tattggatga ctttcaatga
     1981 gatcaactgt gtaaaacatc acccttttgt tagcgtagga gtaattgaag aaaaccatcc
     2041 ccatttggaa caagcgaaat accaaggtgc acaccatcag tttatagcaa gtgctttagc
     2101 tacaaaagct tgtcatgaaa ttatcaaagg ttcacaagtc ggttgtatga tcagctatca
     2161 gcttcttgtt ccgtattctt gtgatccgga tgatattcaa aaaacaattg agcagcaaag
     2221 aacttctttg ttctttagtg atgttcaagc aagaggatat tatccagcgt atacgcaacg
     2281 aatgtttgaa gaaaaaggtg tcaacttaaa aatagaagta ggtgatgaag aaatcttagc
     2341 agcctaccct gttgattttg tttcttttag ctactacatg tctagtgcag tcagtgctca
     2401 tccagaaaat ttggaagggg tccaaggaaa cttaataact ggaggaatta aaaatccgta
     2461 tcttccatca agtgaatggg gctggcagat tgatcctaag ggtttgagaa ttgctttgaa
     2521 tcaattatat gataggtatc aaaaaccact gttcatagct gaaaacggac tcggagctat
     2581 agacaaagta gaagaagatg gaaccgttca agacgattac cgcattgact atttgaagca
     2641 gcatattgag cagatgaagg aagctatcga agatggagta gatttatttg gttatacgtc
     2701 gtggggctgc attgatatcg tcagcgcttc tacttcacaa atgtcaaaac gttatggata
     2761 tatctatgtt gatcaagatg acgaagggaa tggcacaaag aaacggttaa agaaaaaatc
     2821 atttgattgg tacagacaag tcatccaatc aaatggcgaa atcctataat aaaaggaaat
     2881 aagggactgt gacaaaagtc ttgtcacagc cccttcttta cgaataaaca gtattctcct
     2941 gccaactcct cgatcacaag tcaccatagt cataaaacat gagaagctgt tttttgaaat
     3001 tgctccttgt gactcggagt tcaacggcag gctcacgacc tctcaataaa cggtgtacaa
     3061 tcagctgacc atcggcatta gctcaaaaaa cgacaaaata tgaggaacta ttttggtttg
     3121 gctgttctta ttacttgatg caggacggct ttttcacaac ctctgatata aacggtgttc
     3181 aaaagccagt ttttcggtat taagccaaat ttatgcaaaa atgtggaaag ccattttcac
     3241 aaaatttttc ttcatactca aaactaaacg actttttcac aacctctgat tcacggtgtt
     3301 ccagaaggag ttttgtcgaa aacaagccga aattctttga agatggctac ttcttttttt
     3361 caaatctaga cagaggaggc ggacattatt ccgtgttttt tgacaaaaaa taaaaatcat
     3421 actatgatat gtacgccaat ggaggggatg aaatggaaaa gccaattgaa aaagcgttag
     3481 atgaatgtct tttctttagc gtgaaaaagc tagatcgcat gttgaataaa ttagctgatg
     3541 aagcgtttag aaggacaggt ctggcaccaa cttatggttt tattttattg atattaaaag
     3601 aaaaagatgg aataccacaa aaagatattg cacaaatgct ctattccgcc ccatcaacga
     3661 ttgctcgttt tgttgaaaaa ttagaatata aaggatacgt caaaacagtt agcgaaggtc
     3721 gcttgtcatt agtttatctt acagaacaag gacgccaatt tgctaaggaa gtcgatggta
     3781 gctgggaaga acttcatcag tcatacaaag cagttttggg aaatgaagca agtaaagaat
     3841 tagcccagac aatgaatgat gcaacagata agttacaaaa aaaataaaat tttttaagca
     3901 aattagttgc atgtacaact aatcgagtgg tttaatgata gatgattctc gataggttaa
     3961 tcagagcgaa ttatgggagg aatgacaatg tcacaatttg atcaatcaat tactttacct
     4021 tcaggagttc aattgaagaa ccgattgatg ctgtcgccta tgacgacaca actcagcttt
     4081 ttcaatggag tgatcaccga agatgagatc acttattata aacaacgttc taaaggtctt
     4141 ggtgctgtta ttactggtgc tgcaaatgtc caagatatcg gaaaaggctg gccaggtgaa
     4201 ctcagcatcg ctcatgatga aatgattcct cgattaagtg agctagcgaa agcaatccaa
     4261 ggagaaggcg caaaagcgat tattcagatt ttccacggtg gacgaatgac aagtcgagca
     4321 actttatctg gagaacagcc tgtatctgca agtgcagttg cagcaaaacg tcctgatgcg
     4381 gaaacaccac gagcaatgag tgaagaagaa atcgttgaaa caatccaagc atttggcaaa
     4441 gccacacgta gagcgattga atcaggattt gatggaatcg agttgcatgg agcaaatacg
     4501 tatttgatcc aacaattttt ctctccgcat tcgaatagac gagaggatca atggggcggt
     4561 agcttggaaa atcgttatcg ttttattgaa gaattattaa caacggtttt ctcagcggtt
     4621 gaacaatatg ctaaaaaacc attcatcgta gggtatcgtt tctcaccaga agaatatgaa
     4681 acaccaggta tccgttttga agacacgatc tggcttcttg aacgattacg tgagtcaaaa
     4741 ctagattatc tgcatgtttc attaaatatg tatgatcgaa tagctcgttc tgataaatac
     4801 aacgataaat cgattcttga agtggtccat aacacgattc aaggaaagat ccctttagtt
     4861 ggtgtcggtg ggatccgcaa tcgcgaagat gtagagcagg tattgaaata tgcagaactt
     4921 gcagcaattg gacagcaaat gatcgtcgat cctgactggg cagagaaatt attggaaaac
     4981 cgtgaggatg aatttgtaac gaaacctttt gaagaagcat ataaagagct ttatttgcca
     5041 agcccattat ataatttctt gaaagcacgt tatcaataac gcaattttta tagaaagagg
     5101 atgggaaaat gaaagtattg atcgtattaa caaatgtaga aaagtacgaa aagatcgagc
     5161 gtcctacagg tttgtggtta agtgaactga ctcattttta tgatgtatta gtaaaaaatg
     5221 ggatcgatgc agattttgtc agtcctaaag gcggatatgt acctttagaa ccgcaaagtg
     5281 ttttgaatat ggatgaaatc gattgggcct attatgaaga tgaagatttc agaaatcggg
     5341 ccttgggaaa taccttacgt cctgatgatg tggatgcaaa tgattatgca gcggtttatt
     5401 atgctggtgg ccacggaaca atgtgggatt ttccagaagc aatagatctt gggaaagtcg
     5461 cttcacaaat ctatcgaaat gaagggctgg tctcagctgt ttgtcacggt gttgtaggat
     5521 tgttgccagt tacagatgag tctggaaaat ctattttagc tggacgtaca gtgactgggt
     5581 tcagtaatga agaagaggaa gcaaatggta caacgaatga agtaccattc ttagcagaag
     5641 atgctttgaa agaaaaagga gcaaactatc agtctggagc agctttttca gaagtggttc
     5701 aaattgatgg gcggcttctt acagggcaaa atcctcaatc tgctcatcaa ttaggcgaag
     5761 cagttgttga agcattaaag aattaaaaat aaatgatgtg tctattctaa ctattacatt
     5821 acacacgcta aaactcgtct tttcgttctt ttctgtttcc tatttggaca gaggtacgta
     5881 aaaggcgagt ttttttcttt tataagagta cattttttga aacatctctt cagaaatgat
     5941 actcttcaat taggtccact agtacattat tgagaagcag acctagaaca cggtatgatg
     6001 gagagggttg attctgctgc atcgctatgt atcataaggt tgaaagcgag ttatgaatac
     6061 tgtgttttct gattgagaag ttagcagtag aacaccttat aagaaagagg aatgcagatg
     6121 aacattgtaa ttgtcggcgg gtcgtttgca gggattcatg cggctattta tcttcgccaa
     6181 ttgatgccaa caagtgaaat atatttgatt gaaaagcaat cgaagttagg atggattccc
     6241 agcgggttca atttgatatt aaaaggaaag gttaccagtg aaaaacaact ttcttggatc
     6301 acgaaagaag aactgactga ccgctatcga atccatgttt acacagaaaa gacagtaata
     6361 ggattgaaag aaaaaaaagt cgttttagca ggaggaaaaa aattggattt tgatcgtttg
     6421 attctagcga ctggttcaaa tcaaaagttt cgaaatattt ctgcagaatc aacgcttatt
     6481 cacccagtta aaaagataca aaacgtaacg gctcttcagc aaaagatcat gaatgcaaag
     6541 aaaattgcaa ttattggtgc aggacaagca ggaatcgaaa tagctgaagg gttggcatca
     6601 agaaaaaaac aaatccatct ttacgaaagc cgaaagtcaa ttctttttcg ttatctagat
     6661 cctgaaatga cagaaccgtt agtaaaggaa atgaaaaatc aagggctttc acttttttta
     6721 gaagaacaag ttgtgtcttt gaccgaaaat gaaaccgcta ttgtagagac ggaaaaaaga
     6781 aaggagaact atgatcttgt tttgctggct aatcattctc gccccgacaa tcagatgtgg
     6841 gaagaacaat taacattgaa tgatgatgga acgatctggg tagatgacta cttgcaaacg
     6901 tctcaaaaag acgtttatgc gattggcgat gcgatccaag taacttttcg tccaacaaat
     6961 gaaaaaatgt atgtttctct agtaaataac gctgtccgta cagcacgaaa tgtcagcaag
     7021 acaattagtg gctcacagac aaaggatcag ggaacctatc gtccagtcgg caatcagtgg
     7081 tttggctatt tcttaggaag cgtaggtttg acagaagaag agagcatttt ttatccccaa
     7141 aaaattgaaa caggatacgt agactacaag cttacagcaa taaatcaaag caaagttaaa
     7201 ctcaaatttc tatttgattc tgaaaaaaag attcttggag cacagctcca aagcaaaaaa
     7261 gagatgttcc accttcttga ccaactgaca attgcagtaa aagaaggatg gacgtttgaa
     7321 cagttagaag aacatgagct atttttccag ccagaatacc gtgtgccaga tatggtgtgg
     7381 tcaaaggtgg cgaatactgc ctatgaagat tgaagcatta ttagaaagaa aagagcagtt
     7441 acagatcctg attttgcgaa accttgttct gcaaggggga acagcctcta tcaatggttt
     7501 gagagagcat gtccaactgt caaaagcctc gtttgatcaa tatttggaag atattgaatt
     7561 gattggccga atgatggaaa aaaaagtaga agtacagcga aatgagtatc aggcgctgct
     7621 tgtgttggac gaagatgtaa gtttggaaaa aattctattg tttttattgc aggaaagctt
     7681 gaagtttaag atgctcgtct atttattaga acatcagcaa gtatccatcg ttcgattagc
     7741 gacagcgttt aatatcagtg agtcttctgt ttttcgaaag atcaaagaat tgaaccagct
     7801 cttagaggaa tttggattac aaatcaaaaa cggtcaatta tacggagaag agctacagat
     7861 acgatatttt tattatgaac tattccaata tatcccggaa gatcagcgac cgcttttttt
     7921 gcaaaataca ccagaaaaaa gaccgttcat tctaggattg gaccgtgtat tagaaacaac
     7981 gtttacggca tcagcagaag cacagattgc ttgctggctc gggattacga aaaaaagact
     8041 attgaatgaa aaaagtacgt acgcaacttt gaaagaaaag aaattactct atcagtcgga
     8101 ccggctttat caagcaatcg atccaatcat cgtgatgcat cttagtcgaa cagcagcaga
     8161 actgaatgta tacgaaacaa tgatgtttta cagctttttt gtttcttttt cgatagtaga
     8221 cgaagaaata ttttatcagt atgatttgac acggagcaaa aagttaccaa ccgctgtttt
     8281 agatacttac attcgggaaa cgatgttatg gcattatcgt cctcgtcgtt tgaaaatcaa
     8341 agaagaaaag gcagtaggct accaaatttc ccagatcaac aatgaatggt ttttctttgt
     8401 tggaaagatc gaagtctatg agcgtgatcg gcttttggaa cagcagcaga aaatgctcgg
     8461 gcattcctta acacagttac tggcgaaatt acaggaaaca gcggtgcagc aattgccgag
     8521 gaaaagagct gaagatagtg agcttagtta cctgatgatc cagtatgcca atgttctttt
     8581 gatgatcgat ttttatattg caaaaccagt agtgattgga atcgatttag aaagtctgcc
     8641 gatttatcgt attgcttttc aacaatatct gatcagagaa ctgaggggaa tcggcggtat
     8701 tgagattgga agttatgaag aaggtaaaga atatgatttg gtgatcacat tttgccagag
     8761 aaacaaaaaa cagtgtgaat attatctgtc agaatttgcg tcaccttacg acattattcg
     8821 attgaaaaga agaatagaga cgctgaaaaa agaaaagaat tgattaaaaa atagcttcat
     8881 aataaaaaac tgtcaaaaaa agaaaaaaag aataaaattt tgacagtttc tttttttaaa
     8941 acagtagtat gataagagta gttaaagaaa gcgtttgcat ataatggagg gaatcacaat
     9001 gacagaatca acgtatatca tggcgattga ccaaggtaca actagctcac gagcaattat
     9061 ctttgacaag aaaggaagac acatcggcag ttctcaaaaa gagttcacgc aatatttccc
     9121 gcaagaaagt tgggtggaac atgatgcaaa tgaaatctgg aattctgtac agtcagttat
     9181 tgccggagcg ttcatcgaat cagggatcaa accaaatcag attgctggga ttggcataac
     9241 gaaccagcgt gaaacaacag taatatggga aaaagataca ggacgtccaa tctatcatgc
     9301 agttgtttgg caatccagac agtcagctgg aattgcagat aacttaaaaa atgaaggcta
     9361 tcaagaattt ttccacgaaa aaacagggtt agtgatcgat gcctatttct cagcaacgaa
     9421 gatacgctgg attcttgatc atgtagaagg cgcgcaagaa cgagctgaaa aaggcgaatt
     9481 gatgtttgga acgatcgatt cttggttagt atggaaactg accgatggac aagcacatgt
     9541 tactgattac tcgaatgcca gtcggacgat gttgttcaat atccatcagc tagattggga
     9601 tcaggagatt ttagatttac taaatattcc tcgagtgatg ttaccaaaag tcacttctaa
     9661 ctcagaagtt tatggttata cgcaaggtta tcatttttat ggaagcgaag caccaatttc
     9721 agggatggca ggagaccagc aagccgcatt attcggacaa atggcattcg aaccaggcat
     9781 ggtcaagaat acttatggta caggttcatt tattgtcatg aataccggtg aaaaaccgca
     9841 attatccaaa aataatttat tgacgacaat tggttacggc ataaatggaa aggtttacta
     9901 tgctttagaa ggaagtattt ttgtggctgg ttcttcgatt caatggctga gagatggact
     9961 tcaaatgttg caaaaagcta gcgattcaga agacgccgcg aaacgctcta caagcgaaga
    10021 tgaggtttat gttgttcctg cattcgttgg gctaggtgct ccttattggg atcaggcagc
    10081 acgaggggct atgtttggct tgacacgtgg tacgacaaaa gaagatatca ttaaagctac
    10141 tttacagtcg attgcttatc aagtgagaga tatcatcgat acgatgcaag atgacactgg
    10201 aatcaagatt cctgtattga aagtagatgg cggtgcagca aacaatgaat atctgatgca
    10261 attccaaaca gatatcttga atgtaccgat ccaacgagca gaaaacttgg aaacaacggc
    10321 gctaggggca gcatttttag caggattagc tgtcggttat tggaaagata cagacgagat
    10381 ccgcgaattt tatgaagctg gcaagctatt tgaagttcaa atggaggaag agcgccgaga
    10441 aaaactttac aacggttgga aaaaagcagt aaaagctacc caagcatttg aataaggagg
    10501 aaatccatat gttttcaaac aagacaagac aagatagcat tcaaaaaatg cagcaagaag
    10561 aattggatct gttgatcatc ggtggcggaa tcactggtgc cggtgtggca gtccagtcag
    10621 cagcatcagg aatcaaaaca ggattgatcg aaatgcagga ttttgcagaa gggacttcct
    10681 ctcgctcgac caaacttgtt catggcggta ttcgttatct gaagacattt gatgtggaag
    10741 tagtagctga cacagttggt gaacgtgcag tcgtacaaag tattgcccca cacatcccaa
    10801 aaccagatcc aatgcttttg ccaatctatg agggcgaagg agcaacaacc ttcaatatgt
    10861 tctcggtcaa agtagcaatg gacctttatg acaaactggc aaatgtgaca ggaaccaaat
    10921 atgagaatta tacgctgaca ccagaagaag tattggaaag agaaccgttt ttgaaaaaag
    10981 aagggctaaa aggcgcgggt gtctatctag atttccgcaa caatgatgcc cgtttagtga
    11041 ttgataatat caaaaaggct gccgaagatg gggcttatct agtaagtaaa atgaaagcgg
    11101 ttggcttttt atatgatggc gatcaaatcg ttggcgtcaa agctcgtgac ctgctgacag
    11161 atgaagtgat cgaaatcaaa gcaaaattag tgatcaatac gagtggtcct tgggtagata
    11221 aagtacgaaa cttgaatttt acgcgtccag tctctcctaa aatgcgtcca actaaaggga
    11281 tccatttagt tgtagatgcg aaaaaactgc ctgtaccgca acccacatac ttcgatacag
    11341 gaaagcaaga tgggcggatg gtttttgcta ttccaagaga aaacaagact tactttggta
    11401 cgacagatac ggattaccaa ggagatttta cggatccgaa agtcacacaa gaagatgtag
    11461 attatctatt ggatgtgatc aatcatcgct atccagaagc aaatatcaca ttgaatgata
    11521 tcgaagcaag ctgggcagga cttcgtccac tgttgattgg taattctggt tctgattaca
    11581 atggtggaga taatggatcg atttcagaca agagcttcaa taaagttgtc gatacagtaa
    11641 gtgaatataa ggaaaacaaa gtttctcgtg ctgaagtaga agacgtgttg aaccatttgg
    11701 aaaacagccg tgatgaaaaa gcaccttcta cgatttccag aggtagttct ttagaaagag
    11761 aaccagatgg tttgctgact ttatctggtg ggaaaatcac cgattaccgt aagatggcag
    11821 aaggagcttt gcgattgata cgccaattat taaaagaaga atacggaata gagacgaaag
    11881 aaatcgactc taaaaaatat cagatttcag gtggaaactt cgatccaact aaattagaag
    11941 aaacagtgac agaattagca aaagaaggag tagcagccgg tttagaggaa gaagatgcta
    12001 cttatatcgc tgatttttac gggactaatg cccgacgtat ctttgaatta gcaaaagaaa
    12061 tggcacctta tcctggtttg agtctcgctg agtcagctcg gttacgttat ggtttagaag
    12121 aagaaatggt tttggcccca ggcgattatc tcattcgtcg tacaaatcat ctgttgtttg
    12181 aacgagatca gcttgatgag atcaagcaac ctgtgatcga tgcaattgct ggatattttg
    12241 aatggacaga agaggagaag gcgcaacaga ctaaacgttt agaagcattg attgcagaat
    12301 cagatctgcg ggaactaaag ggggagaaat aaatgaacag tgatatgact caaatcatgg
    12361 gggagtttat tgggacactg atcctagttt tgcttggaga tggtgtgtgt gcggctgtga
    12421 atttaaacaa aagtaaagca caggcttctg gctggattgt catcgcattt ggttggggac
    12481 tcgctgtaac aatggctgtc tatatttctg gattcatggg cccagctcat ttgaatcctg
    12541 cagtctctct agctatggcg atgacagggg caattagttg gaatcttgtc gttcctttca
    12601 tcattgcgca agttttagga gcttttgcag gggccattct tgtttggctt tcttatttgc
    12661 cacattggaa tgcaacgaaa gatgaaagcg cgattttagg aacgtttgca acaggtcctg
    12721 ctatacggaa ttatccagca aatgtgatca cagaattaat tggtacattt gtattagttc
    12781 ttgggttgct agcttttgga caaaacgaat ttgcacctgg aacgaacgta ttcgctgtag
    12841 gtggattgat cttagccatc ggtctttcat taggagggcc aacaggatat gcaatcaacc
    12901 ctgctcggga ctttggtcct cgattagcac atgcggtatt gccaatagct aataaaggaa
    12961 cttcagattg ggcatattca tgggtaccaa tcgcaggtcc aatgatcgga gcaattatcg
    13021 cggtaggtgt ctattcatta atggtgtaaa tctataaaac atactaaagt ttgacaacca
    13081 aaatgtgtct tgctatagtt aagtggaaaa taaataagac ctagaagaat ggggtatgtt
    13141 ttgtgagtgt agctggtatc taatcagtaa gtcgaatagc caataaacaa agtttatttg
    13201 gtgtgcttat gaaacggtac ctgtcaggaa aacaatatct ttgtattcta ataaggatag
    13261 gacatgactt ttttaagctg tgttctatcc ttttatttat ctctaggccc ttatttattt
    13321 tgggacaggt atctcttcaa gaaaaggaga gaatatggac aacgaaatga ttagtcaatt
    13381 acaatttgat cggatcaaaa aggaaatcca agcaagagca attggaaact atagcaaaaa
    13441 acgaatcagc gatatgacgg tctcgacaaa cctacaaact gtcttggatc gtcaagaaga
    13501 aacaagagaa gcacgcttga tcttagaaag cagtcagcat gttccattta tggggttgcc
    13561 gaggatcgat gcactgacag aacaagtcaa aaaagggttg attctgcagc caaccgattt
    13621 gatcgaatat gcggattttc ttagaagcag tcggatgatc acaaagtttt ttgataagaa
    13681 tcaataccag acacccttgt tgtttgctta tagcaaacat cttcctgatt tgataaatgt
    13741 agaagagttg attgaccaga aaataaaaaa taataaagtc agtgacgacg cgtcacgtaa
    13801 tttgcgtaaa gtacgcaaac aattacaact aatcgaaaaa gaaatccagt caaaattact
    13861 gaaattcttg cgtcatccga aaaacaaaga aatgatccaa gaagcaatga ttgttcaaaa
    13921 aggcgaatac tatacgattc ctatcaaagc cagctataaa aataaaatag atggaacgat
    13981 tatcgatgaa tcaaataaaa gaacgacggt ttttattgaa ccaactgtga tttctaaatt
    14041 gaatgaacac tatcagttgt taaaagctga agaaatcagt gaagaatatc aagtcctggc
    14101 ggcattgact ggtgctatcg cagaaaacga agaagtgatc gatttattga tcgaaacgat
    14161 cacggtgtta gatatcatct ttgctcgggc aaaatttagc cgagaaatca atggaagcac
    14221 acctagaatc aataaatcgg aacatatcat tatcaagcaa ggcagacatc catttttacc
    14281 tgagcatgcg gtaccattgg atgttgaaat cggtaaagac tatcgaggct tgattatcac
    14341 cggagcaaat gccggaggca aaacagtcgt gttgaagaca gtcggtttat tgactttgat
    14401 ggcgatgttc ggtatgcaag ttccggcaaa agaagggaca gagcttgctg tctttgatga
    14461 gattttcgtc gatgtcggtg accaccaaaa tttagaaaat gcgttgagta cattttctgg
    14521 tcatatgcaa aacatcgctg ctattttgaa aaagataaaa aggaatacgt tggtactttt
    14581 agatgagatt ggcagtggaa cagagccaaa tgaaggggct gcactagcga tcgcaatcat
    14641 ggaatctatg tatgagcaag gggcattgat cattgccacg acccattatg gagaaatcaa
    14701 gaaattcgcc ggagatcatg aagattttgt gccggctgct atggctttcg atcgggaagc
    14761 acttcgtcct aaatatcagt tgcgggttgg agaaacagga gaaagccaag ctttgtggat
    14821 tgctcataaa atggatatgt cgatgaaatt gatccatcaa gcagagcgtt acttggcgga
    14881 aaaagcctat cggacacaga aaaaagaatt tcttcctaag acagcagtta caaataggaa
    14941 ggagaataaa aaagaacgcc agctctttgc aaaaggtgat cggatatttg tcaatgaata
    15001 ccaaaaagaa gcgttagttt atgaagatat aggtgaagat acgattgatg tatatttaga
    15061 caaaaaaata atccacgtgc ctcgtcagcg tgttcgtctg gtgcgttcag cagaagatct
    15121 gtacccgaca ggttacgatt tagacagtct gtttatcgat tataaaacac gtaaacggca
    15181 gcgtgaccta gagcgaggat caaaaaaagc tcataaagta ctagtaaaag aaatgcgcaa
    15241 acgacaggaa gaaagaagag taaacgatga aaacagcaag tgattatctc aaattagctc
    15301 ataatatcag tggctgtaaa gaaaaataaa aacagtgaaa tcattggttt tattccccgt
    15361 gtaagctaaa ataaaaagaa aagagtgctg aggaggtagg gcgataaaat gaaattaagc
    15421 acagactggc gtaaagaaat acaaacgatt ccgaatcttt tatcgatatt ccgtattttt
    15481 ttgctgccga tttatcttta ttttgtgctg agacagtcat tttatatagc aggagcggtc
    15541 attgttgtct caggtttgtc tgattatcta gacggggtca ttgctcggag atataatcag
    15601 gtaacagact tagggaaggt gcttgatccg tttgcagata aattgacaca actttttctg
    15661 attttgtcta tggcatggta tcgaccgtgg ctttggctgt tgtttggtct attcttgata
    15721 aaagaaggct ttatgtttgt agctggatta atcgggctat ctaaaaatat caaattaagt
    15781 ggagcgaagt ggtatggcaa agtagcgact gctgtcatct atgttggcat gatccttttg
    15841 ctgcttttcc cagaattacc aacgctgtgg gtgagagtca tttttgccgt catcacttac
    15901 ggtcttttac aatcttttgt cttgtatgct gtcgagtacc gaaagatgtt tcaaagaaaa
    15961 taaaaaaggg gtgtgaccta tgtcgcattc ctttttttga taaatagact cccagtagct
    16021 acttctcgat aataagccaa ccaaaccaaa aacaggagaa gttgtttttg aaattatttc
    16081 ttgtgattcg gagttaaatg gtaggctcac aacctcttgg taaacggtgt tcaatcagct
    16141 gaccttcggt attagctcaa aaaacggcaa aatatgagga gctattttcc cgtttttttc
    16201 tctaatactt aggtcaaacc gctgatttca caacctcttc cttaaacggt gttcaaaagc
    16261 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agaagctatt tttgtttggc
    16321 tgttcttatt acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga
    16381 agtagcttct tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct
    16441 ctactgacta aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga
    16501 cacctgctgg tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac
    16561 gcatagcatt ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg
    16621 cagagtctag ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg
    16681 gagaagcttt tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat
    16741 caatatggaa aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc
    16801 gcttgttcta gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt
    16861 ttcagattat ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt
    16921 aattgagtga tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga
    16981 atttgtccct ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca
    17041 cattctcctc tatttttata attagataat accccttatc ggtattttat gcaaatataa
    17101 aaagtagtga aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa
    17161 tataccccat gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt
    17221 caatcaataa aatcgataac agtcccagaa ttaaaggaaa agcttttgga aaatccagcg
    17281 ttacttgatg ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat
    17341 gttccattac aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt
    17401 tgtcaatcag ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat
    17461 gtagtgaatg tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa
    17521 gatgaaaatc gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg
    17581 ccgattaatg gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc
    17641 aaattgcggc ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt
    17701 ccaaacgcca gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt
    17761 gacagccatc tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat
    17821 tgaacagtac gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc
    17881 agggataaca gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt
    17941 gatgcatgca ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg
    18001 attggaaatg gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc
    18061 accacatatt cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc
    18121 tcatcaagga gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa
    18181 acgattgcga ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg
    18241 tgttcgtcct gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg
    18301 tatcctagta gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc
    18361 tatcgttgta aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc
    18421 caatcgtcaa ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg
    18481 cggtattgga acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag
    18541 tgaaagaaca gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga
    18601 tcatgcaagc tattatccag aagcaacaga tattttactg aagttgatct tccatccaga
    18661 gactggcgag atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga
    18721 tattttagca acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt
    18781 gacgtatgca ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc
    18841 aatgaacatt gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact
    18901 agcaaaagga aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt
    18961 caaggaagcc aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag
    19021 ccaacaagaa tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat
    19081 cttgaagcag tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac
    19141 tgtccgacct gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc
    19201 gactcttaaa agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta
    19261 tggtcaacac acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa
    19321 ttgataaaaa aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt
    19381 cgttgaaaga aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg
    19441 atactttttc gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga
    19501 aaaacgcaga aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa
    19561 aagtcgttgt agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa
    19621 atttcacaga tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac
    19681 ttttttacaa taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata
    19741 cggttattaa agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg
    19801 atacgtctgt agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca
    19861 atgaaatgaa ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg
    19921 tgaaaagtca aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca
    19981 tgcagcgtat tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc
    20041 tgattctatc acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag
    20101 gtgaaagaaa tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg
    20161 aaatagaagg tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga
    20221 cagaacgttt gcgtagcaaa aagtttatcg ataatctaaa acaatcagat cctttgtttt
    20281 tagatcatcc gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa
    20341 aaacgatcga aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc
    20401 aagcaacgaa aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa
    20461 gtcctgctgt gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg
    20521 gtaaattgat ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac
    20581 aaaaaaaata tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc
    20641 agttgtttga aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac
    20701 gtactgttga tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa
    20761 atgtaccagc atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat
    20821 taggtctatt gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc
    20881 aggcaatctt taattcttcc actacttttg ctgatattat gcgttgtttc gatgactacc
    20941 atctgccgat ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag
    21001 agcttttcaa aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc
    21061 aaggaaaaga acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag
    21121 ctgagatttt ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg
    21181 aaacccaaac gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa
    21241 tcattccttt gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg
    21301 aaaacgatgg agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa
    21361 tgaaaagcat tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac
    21421 aactgataaa aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc
    21481 ttaggaaaaa tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac
    21541 gtcgtaacaa aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat
    21601 caattttggt gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa
    21661 cgttagtggc tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc
    21721 cattaacgag accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga
    21781 aaatgctgta tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct
    21841 catttgtatt gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg
    21901 aat
//
LOCUS       DAGCWU010000028        21583 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.028, whole genome shotgun sequence.
ACCESSION   DAGCWU010000028 DAGCWU010000000
VERSION     DAGCWU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.028"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(595..1986)
                     /locus_tag="MBZ71_000861"
     CDS             complement(595..1986)
                     /locus_tag="MBZ71_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBT4428832.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAVAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2199..2585)
                     /locus_tag="MBZ71_000862"
     CDS             complement(2199..2585)
                     /locus_tag="MBZ71_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBT4428833.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2641..3264)
                     /locus_tag="MBZ71_000863"
     CDS             complement(2641..3264)
                     /locus_tag="MBZ71_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBT4428834.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3328..3996)
                     /locus_tag="MBZ71_000864"
     CDS             complement(3328..3996)
                     /locus_tag="MBZ71_000864"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBT4428835.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4008..4448)
                     /locus_tag="MBZ71_000865"
     CDS             complement(4008..4448)
                     /locus_tag="MBZ71_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428836.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4507..5178)
                     /locus_tag="MBZ71_000866"
     CDS             complement(4507..5178)
                     /locus_tag="MBZ71_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428837.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFLPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(5363..5761)
                     /locus_tag="MBZ71_000867"
     CDS             complement(5363..5761)
                     /locus_tag="MBZ71_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBT4428838.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5758..6531)
                     /locus_tag="MBZ71_000868"
     CDS             complement(5758..6531)
                     /locus_tag="MBZ71_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBT4428839.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6528..7211)
                     /locus_tag="MBZ71_000869"
     CDS             complement(6528..7211)
                     /locus_tag="MBZ71_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBT4428840.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7378..8265)
                     /locus_tag="MBZ71_000870"
     CDS             complement(7378..8265)
                     /locus_tag="MBZ71_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4428841.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8262..9131)
                     /locus_tag="MBZ71_000871"
     CDS             complement(8262..9131)
                     /locus_tag="MBZ71_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4428842.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9268..10554)
                     /locus_tag="MBZ71_000872"
     CDS             complement(9268..10554)
                     /locus_tag="MBZ71_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4428843.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10827..11405
                     /locus_tag="MBZ71_000873"
     CDS             10827..11405
                     /locus_tag="MBZ71_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4428844.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11405..12334
                     /locus_tag="MBZ71_000874"
     CDS             11405..12334
                     /locus_tag="MBZ71_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4428845.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12331..14067
                     /locus_tag="MBZ71_000875"
     CDS             12331..14067
                     /locus_tag="MBZ71_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBT4428846.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14130..14966)
                     /gene="pheA"
                     /locus_tag="MBZ71_000876"
     CDS             complement(14130..14966)
                     /gene="pheA"
                     /locus_tag="MBZ71_000876"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBT4428847.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14963..15472)
                     /locus_tag="MBZ71_000877"
     CDS             complement(14963..15472)
                     /locus_tag="MBZ71_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBT4428848.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15465..16760)
                     /gene="aroA"
                     /locus_tag="MBZ71_000878"
     CDS             complement(15465..16760)
                     /gene="aroA"
                     /locus_tag="MBZ71_000878"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBT4428849.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16773..17864)
                     /locus_tag="MBZ71_000879"
     CDS             complement(16773..17864)
                     /locus_tag="MBZ71_000879"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBT4428850.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17881..19047)
                     /gene="aroC"
                     /locus_tag="MBZ71_000880"
     CDS             complement(17881..19047)
                     /gene="aroC"
                     /locus_tag="MBZ71_000880"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBT4428851.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19049..20113)
                     /gene="aroB"
                     /locus_tag="MBZ71_000881"
     CDS             complement(19049..20113)
                     /gene="aroB"
                     /locus_tag="MBZ71_000881"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBT4428852.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20146..21168)
                     /gene="aroF"
                     /locus_tag="MBZ71_000882"
     CDS             complement(20146..21168)
                     /gene="aroF"
                     /locus_tag="MBZ71_000882"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBT4428853.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         6246 a         4717 c         3770 g         6850 t
ORIGIN      
        1 gatctgttct aacattctca actttgtcag tttaccatag gaaaatggga aaatctacga
       61 aagctctcaa cagaatcgtc ctacttaaat acgcttttct ttaataataa ataagttggt
      121 tgtttttgtt atttgcgctt tcacttttcg gaataaataa tgtgtattta tatggaataa
      181 ataaaaaaag aggtcgtgac taaaaacaag tcacaacctc tggtaaacgg tgttctcctg
      241 acaactcctc ggtcacaaga cacaatattc aacaaacatg agaagctatt ttttgaaatt
      301 gctctttgtg actcagagtg aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa
      361 aagcttgcat ctgcggtaat aagctcaacc aaacgtaaaa tatgaggaac tatttttgtt
      421 tggctgttct tattacttga tgcagggcag ctttttcaca acctcttatt cacggtgttt
      481 cagaagtagc ttctcggaaa taagccgaaa tttcacaaaa atttgagaaa ctatttttag
      541 gaaacttctt cttatttctt aaagctgacc acttctatca caacctcttt ttgactatgc
      601 attcacatcg atcactttag aatcatcatt gatgacatcg cgatctaaat ctttgatgcg
      661 tgcacttgtc acgataccag ctaccatgct accgctgacg tttactgctg tacgtgccat
      721 atcgattaat ggttcaacag agataactaa cccaacgatt gctactggca agttcattgc
      781 ccctaaaacg atcagtgaag caaatgttgc gcccccaccg actccagcta caccaaaaga
      841 actgattgtt acaattgcca caagcatcaa aataaattcc aaactaaaga cattcactcc
      901 agctgctggt gcgacgatcg ttgccaacat agctggataa acgccagcac agccgttttg
      961 tccaatagac aaaccaaaac ttgctgagaa attagctgtt gcatcatcta ctcctaaagc
     1021 ttttgtctga gtctcgatat tcaatggcaa agctccagca cttgaacgtg aagtaaacgc
     1081 aaagctcaat accggaaagg cttttttgaa atagttgatt ggattgactt ttgcaccaat
     1141 caaaatcagt gagtggacaa tcaaaacagc aagcaatgct acgtaagaag caacgacaaa
     1201 tttacctaaa ttgacgatag ctgcaaaatc acttgttgcc aaaacgtttg tcatcaaagc
     1261 aaatactcca taaggtgtca aacgcaaaac aagcgtcacg atccgcataa tgattttata
     1321 cagactgtcg actaaatctg aaaagaatgc tgcttctttt ggcgcttttc ttttcacgcc
     1381 tagataagcg attccgacaa atgccgcgaa aataacgact ccaatcgtac ttgttgcacg
     1441 actacctgct aaatcagcaa aaacgttttt agggataaag gataaaatct gctgtggaat
     1501 cgataagttt tcaactgttt cctgacgtgt tgccagttca gagattcgtg ctgtttctgc
     1561 agctccttgt gtgaatttag ctccttggag tccgaacagc attgttgaac caattccgat
     1621 caatgctgca acagctgttg ttccaagtaa agaagtcaat acagtcgcac taattttccc
     1681 tagcttttca gaaactttca tttttgtgaa tgcaccaatg atcgaaataa acactaaagg
     1741 cataactagc atttgcaaga atgatacata tccttgacct acaatcccga tccattccat
     1801 tgattgagta acgactttgt cgtctacacc aaagatcaat tgaagcagac cgccaaagac
     1861 gataccaact cctaatgctg taaagacgcg tgttgaaaat tttgtatggc gcttttgcat
     1921 acgatagaac acatataata atgccgcaaa aactagaacc actaaaactg tgatgagtgt
     1981 tgtcattttt ctcctcctca tattattaat gacaagtcca tcgaaggaag taaaggttcc
     2041 cgagaatgat tcttcaacca tcctcctgaa aaaatttttt tcagtgactt ttatatttta
     2101 gacgattttt atcgaaatga cattcttttt gcttacaatt agtaataaaa aaacatacct
     2161 gtgatagctt taatctatcg caggtatgtt tttactgtct attcttgttt ttctgattga
     2221 aaattcaatt ttaccccagg ctgatctaag gcgatctctg tcacctcata agacattcgg
     2281 cgtacgatat cgaataacgg tgctactagc tcgtccactt cttcttgtgt gacatcattt
     2341 tgaccttcaa ttttccgatt gaggatatga ttgatctgac tcacacttcc agataaaaca
     2401 tatgtatcaa acactaaacg gaactctaag cgtgctccta cgatgctatg ttctttcgga
     2461 tagttttcga tcgtttcttt taaaggcgag aagcctactt tcaaatctgt ttctacttct
     2521 tggttcggca atctcatatc ataatgaaat gcttctacaa cttctttttg tcttctgatt
     2581 tccatataat tttatcactc ctttatgaaa attataacat atctcctttg aaaggggata
     2641 ctacttttta atgatgctct gcaaggtaag gatttcattc agatttccta ccacataatc
     2701 tgccgggatc tcacccagaa aatgatcgat gtcgtaaaga caagtagcga ctcctgcatt
     2761 tttccctgct tcgatatcta aacgacgatc gccgatcatc atcgtgtctg ttctttctaa
     2821 atgaaaagta tcgatcaaat aatttaaaga tgctggatca ggtttacgag gaaagtcctg
     2881 atcgatccct actacttcct caatcagatg tgccaatcga tgttctttta acaggcccca
     2941 tgttgattct gtcaaacggt gggtcaggat gaaatgacga ccgccgttgt cttttagcat
     3001 ttccagtact tgtttcgttt ctggaaatgg acgggaaact tccgtacttt ctttttcata
     3061 tcggtgaaat aattcattaa actctctggc attcaagttt gattcattga tcaaataact
     3121 agtagaatac tttttcattt tgaagtagat ttcttttttg tccatactga taccaaaatc
     3181 ttttagtgct tgccatgcac catctaccat tgcgggatac gtatcaaaca atgtaccatc
     3241 aaagtcccat atataatttt tcatgttcct tttgttccat tcctttgctt cttttttcta
     3301 tctctcactt ttagataaag gtgttcttta aagtattttt ttgtccagaa ttgtcaaatc
     3361 atctgctaat gtatactgga ttggttgtgc atttgggata tcgatcgtat ccatttgatc
     3421 ttcaggaaca tcttctaaat attttgtcag tgctcggatg ctattaccat gacccacaac
     3481 tagtaaatct tgccgatctt ttagttttgg tgctagatga tcttgccata agggaatgat
     3541 ccgattcact gtcatttcca gactttctcc gtatgggatc aaatgcggat ccaagtgatc
     3601 atatctgcgg tcaaatctat tttctgcggt taacggcgga acttccgtat atcctctgcg
     3661 ccatgttttt acttgatctt ttccgtatat ttctttcatc ttatctttgt ttttacccac
     3721 aagatcccca taatgccgtt catttagacg ccatgttttt attataggaa gccataactg
     3781 acccatttct tctagtgcga cttgtccggt aagaatcgca cgtttcaaaa cagaggtata
     3841 aacggcatcg aattgaagtc cggcttcaag cattttttga ccggcaactg ctgcttgttc
     3901 ttttcctttt tctgttaagg cgacatctag ccatcctgtc cagtaatttt caaaatttgc
     3961 ttcgctttcg ccatgacgta ataatactaa tttcattctt ttctccctta aacattcgtt
     4021 tcgaatttta taccacatgt tttgcatgtc acacgtattc gtccttttcc tttaggtgca
     4081 cgctgctttt gatgacaatt cgggcactca aaaaaagtat aactttttcg atccattttg
     4141 acttgctttt tgttttttat ctgatgatat ttttctttga ctctatcaag atatttcaaa
     4201 taacgttgat tctcatttag tctggggtag attttttttg ataggaaacg aaatatcatc
     4261 catccgaaaa agacgatgaa caacaattgt ccgattctat agggcaacca acgacttagc
     4321 aaactaaaga caagcgcagc gatcaataga ccgcgattca actgatcaaa tttcgcatat
     4381 cttccctgca taagtttgtg atagttctgc tgaaatcgta ttagcctttc taaccatgtt
     4441 tgcatcatat tatctcctcc atcatctaat atttattatg acaaaaaaat ccaaaaagaa
     4501 aaagccttaa ttggcctttt ccattgcaac ttggtgaagg acattccgat ttctggtaaa
     4561 gtcaatcaca atcactttct ttttatcttc tctgttgact actcgaaatg tgtccgtcaa
     4621 tggaagaaac agcttcttag atggtagctg ttgcatacgt ataaaacgat ggatcatcgt
     4681 aatcaattga ggaaattgaa tagacgtgtg aagcagacct ggatattttt tcaacagctg
     4741 tgtcgttttg atcagattcc gttttttact gatcactttt aaaaagcttt ttagaatctt
     4801 ttgctgacgt ctaaaaatag aaaaagaatt ttcgtcatct atttgataac gcatttgttt
     4861 ttgcagttct gctgcagtca gatggatctt cccatcgttt gtatcgatca ttaaaccttc
     4921 tggaaacagt tcaaccactg ctcgatccca agattcaaga gaaagctcga tataatcttt
     4981 tactggtaat tccaattcta ccttaaaaaa gtgcaaaaca gccgacagcc cttctgcttg
     5041 atagatttct cctaatgtta gcccctctag atggaactct ggtaaaaaat gcagcaatgt
     5101 tttattttcc tgcaattgaa catatagaaa tgtagatcgc tcaatcagag tatcttttaa
     5161 aacaactaaa aaattcattt ttctccccct tttttcatca tttttaaaat agcatatttt
     5221 gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt cataagtatt
     5281 cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct tgtttgtgtt
     5341 tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta ttttcatcta
     5401 aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata ccaatgactt
     5461 tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg atgccttgac
     5521 caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac acaatatctt
     5581 tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca gaacctcttc
     5641 catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata acatcttcgc
     5701 ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca agatctttca
     5761 tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt catcttttcg
     5821 tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa tcattgcaac
     5881 cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga ttgccacgct
     5941 cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt tatcaaaagc
     6001 cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc cgtatcctgg
     6061 gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca atcctactgc
     6121 tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc tcccctcgct
     6181 caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc ctactaagaa
     6241 ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg caatagcact
     6301 gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga aaaggatcaa
     6361 cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt tgcgcatacg
     6421 aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg ccaaaatgac
     6481 ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca ttggaagatc
     6541 acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac cattccaaaa
     6601 ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc aagggccaag
     6661 ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc aatcggcata
     6721 aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat agccatcaat
     6781 tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa tactccagtg
     6841 ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga tatatcatat
     6901 acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat cccgatagca
     6961 aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt ccctactaga
     7021 tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat tccgatcatc
     7081 atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg tgcaaaaaca
     7141 aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa
     7201 ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt
     7261 cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc
     7321 aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta
     7381 ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc
     7441 agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga
     7501 agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac
     7561 taagggccat aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata
     7621 ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc
     7681 taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa
     7741 gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa
     7801 aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg
     7861 aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat
     7921 ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc
     7981 gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg
     8041 aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt
     8101 attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct
     8161 taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt
     8221 gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct
     8281 actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct
     8341 gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg
     8401 gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc
     8461 aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc
     8521 gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc
     8581 tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc
     8641 caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc
     8701 ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat
     8761 agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg
     8821 gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt
     8881 gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct
     8941 tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata
     9001 ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc
     9061 atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct
     9121 ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa
     9181 agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag
     9241 cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg
     9301 tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc
     9361 tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt
     9421 ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata
     9481 agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt
     9541 gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc
     9601 tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt
     9661 ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc
     9721 tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga
     9781 ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa
     9841 aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt
     9901 ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc
     9961 aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc
    10021 tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc
    10081 tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta
    10141 tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac
    10201 aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata
    10261 atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt
    10321 cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt
    10381 ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca
    10441 gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa
    10501 acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat
    10561 ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga
    10621 aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac
    10681 aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg
    10741 tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca
    10801 agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac
    10861 agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa
    10921 tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata
    10981 tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa
    11041 aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg
    11101 ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg
    11161 atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa
    11221 gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac
    11281 aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac
    11341 cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc
    11401 attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc
    11461 cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg
    11521 tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat
    11581 caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg
    11641 aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc
    11701 aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga
    11761 gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc
    11821 aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga
    11881 actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga
    11941 tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa
    12001 aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa
    12061 agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc
    12121 catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag
    12181 taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt
    12241 cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc
    12301 ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc
    12361 tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct
    12421 ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat
    12481 gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca
    12541 ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa
    12601 catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa
    12661 atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc
    12721 ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag
    12781 agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt
    12841 cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac
    12901 gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct
    12961 gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca
    13021 tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt
    13081 gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt
    13141 acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg
    13201 attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg
    13261 gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat
    13321 ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg
    13381 atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag
    13441 aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat
    13501 aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa
    13561 ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt
    13621 ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat
    13681 ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg
    13741 aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg
    13801 caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt
    13861 gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga
    13921 atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta
    13981 acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct
    14041 aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag
    14101 ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact
    14161 ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc
    14221 tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag
    14281 ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct
    14341 gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt
    14401 tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc
    14461 tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct
    14521 gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct
    14581 gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac
    14641 tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga
    14701 ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga
    14761 tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga
    14821 acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca
    14881 tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt
    14941 tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga
    15001 tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag
    15061 aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc
    15121 gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt
    15181 aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc
    15241 caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct gactccactt
    15301 ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt
    15361 cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac
    15421 cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct
    15481 cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct
    15541 tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata
    15601 ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg
    15661 atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc
    15721 accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg
    15781 attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga
    15841 atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc
    15901 gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca
    15961 tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc
    16021 ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt
    16081 atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg
    16141 atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca
    16201 acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc
    16261 gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg
    16321 attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt
    16381 tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta
    16441 aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg
    16501 tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc
    16561 tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa
    16621 tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg
    16681 atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt
    16741 atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta
    16801 ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc
    16861 agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc
    16921 gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata
    16981 aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt
    17041 tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct
    17101 gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg
    17161 ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga
    17221 aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga
    17281 ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca
    17341 tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt
    17401 ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat
    17461 agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg
    17521 acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca
    17581 tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag
    17641 gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa
    17701 tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga
    17761 ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa
    17821 gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt
    17881 ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga
    17941 atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc
    18001 aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg
    18061 ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat
    18121 gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt
    18181 attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga
    18241 gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat
    18301 actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc
    18361 taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt
    18421 tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg
    18481 tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc
    18541 tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag
    18601 aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg
    18661 ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg
    18721 tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac
    18781 agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga
    18841 accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt
    18901 catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc
    18961 ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg
    19021 ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct
    19081 ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt
    19141 ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta
    19201 gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc
    19261 cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta
    19321 ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg
    19381 tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt
    19441 ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt
    19501 cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga
    19561 tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat
    19621 caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat
    19681 tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt
    19741 gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca
    19801 ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat
    19861 cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa
    19921 ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag
    19981 caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg
    20041 atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat
    20101 gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg
    20161 aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt
    20221 ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac
    20281 gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca
    20341 aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa
    20401 tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg
    20461 ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca
    20521 ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac
    20581 agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta
    20641 ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa
    20701 ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata
    20761 tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc
    20821 cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt
    20881 cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg
    20941 gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc
    21001 atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac
    21061 tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac
    21121 tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc
    21181 tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac
    21241 attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact
    21301 tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac
    21361 atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt
    21421 ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt
    21481 tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat
    21541 tcagttttgt aagttttcta gatttcataa aaaatcgctc caa
//
LOCUS       DAGCWU010000029        21221 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.029, whole genome shotgun sequence.
ACCESSION   DAGCWU010000029 DAGCWU010000000
VERSION     DAGCWU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.029"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(117..1004)
                     /locus_tag="MBZ71_000883"
     CDS             complement(117..1004)
                     /locus_tag="MBZ71_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBT4428854.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            1189..2028
                     /locus_tag="MBZ71_000884"
     CDS             1189..2028
                     /locus_tag="MBZ71_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBT4428855.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2224..3198
                     /gene="sgrA"
                     /locus_tag="MBZ71_000885"
     CDS             2224..3198
                     /gene="sgrA"
                     /locus_tag="MBZ71_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBT4428856.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            3496..3852
                     /locus_tag="MBZ71_000886"
     CDS             3496..3852
                     /locus_tag="MBZ71_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428857.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            3855..5012
                     /locus_tag="MBZ71_000887"
     CDS             3855..5012
                     /locus_tag="MBZ71_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4428858.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5038..5478)
                     /locus_tag="MBZ71_000888"
     CDS             complement(5038..5478)
                     /locus_tag="MBZ71_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBT4428859.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5638..6144
                     /locus_tag="MBZ71_000889"
     CDS             5638..6144
                     /locus_tag="MBZ71_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4428860.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6312..8225
                     /locus_tag="MBZ71_000890"
     CDS             6312..8225
                     /locus_tag="MBZ71_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBT4428861.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            8230..9231
                     /locus_tag="MBZ71_000891"
     CDS             8230..9231
                     /locus_tag="MBZ71_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4428862.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(9433..9639)
                     /locus_tag="MBZ71_000892"
     CDS             complement(9433..9639)
                     /locus_tag="MBZ71_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBT4428863.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(10285..11133)
                     /locus_tag="MBZ71_000893"
     CDS             complement(10285..11133)
                     /locus_tag="MBZ71_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4428864.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            11132..12199
                     /gene="gnd"
                     /locus_tag="MBZ71_000894"
     CDS             11132..12199
                     /gene="gnd"
                     /locus_tag="MBZ71_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBT4428865.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            12201..13202
                     /locus_tag="MBZ71_000895"
     CDS             12201..13202
                     /locus_tag="MBZ71_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBT4428866.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            13199..13483
                     /locus_tag="MBZ71_000896"
     CDS             13199..13483
                     /locus_tag="MBZ71_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBT4428867.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            13516..14313
                     /locus_tag="MBZ71_000897"
     CDS             13516..14313
                     /locus_tag="MBZ71_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4428868.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HVAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            14327..15160
                     /locus_tag="MBZ71_000898"
     CDS             14327..15160
                     /locus_tag="MBZ71_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4428869.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            15178..16035
                     /locus_tag="MBZ71_000899"
     CDS             15178..16035
                     /locus_tag="MBZ71_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4428870.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            16057..16566
                     /locus_tag="MBZ71_000900"
     CDS             16057..16566
                     /locus_tag="MBZ71_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4428871.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYSVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            16603..17004
                     /locus_tag="MBZ71_000901"
     CDS             16603..17004
                     /locus_tag="MBZ71_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428872.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            17116..17469
                     /locus_tag="MBZ71_000902"
     CDS             17116..17469
                     /locus_tag="MBZ71_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428873.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            17503..17946
                     /locus_tag="MBZ71_000903"
     CDS             17503..17946
                     /locus_tag="MBZ71_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBT4428874.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            17963..19048
                     /gene="uxuA"
                     /locus_tag="MBZ71_000904"
     CDS             17963..19048
                     /gene="uxuA"
                     /locus_tag="MBZ71_000904"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBT4428875.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            19041..19754
                     /locus_tag="MBZ71_000905"
     CDS             19041..19754
                     /locus_tag="MBZ71_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4428876.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(19961..20110)
                     /locus_tag="MBZ71_000906"
     CDS             complement(19961..20110)
                     /locus_tag="MBZ71_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428877.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            20506..20706
                     /locus_tag="MBZ71_000907"
     CDS             20506..20706
                     /locus_tag="MBZ71_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4428878.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7271 a         3258 c         4402 g         6290 t
ORIGIN      
        1 aaataaaaaa aggccccgat gaagttgtgg caaaagtggt ttgcctcaaa aataagaagg
       61 aatttccaaa aatcacttct caaattttgc cacatcccca attgggtcaa atttgtttat
      121 ttgaatatat tttttacata aggatcttta atagttacat tgtctttttt catcacttta
      181 cggattgttt tatccatgaa ttccgtatca agagtttttg cttcggtagc aattttttct
      241 agctcagatt tgtatttgtc tttattagaa cctttatcct gtttttttac catttttact
      301 agataatagc tttgctgata agtcgttgga tctgttacag gaatgatatc agaaacttcc
      361 ccgtttttta gtttaaatgc tgcttttttt acttcagtag gaatctctgg attggttgaa
      421 tcaaaattca tttttccatc agtttccttt aatgttttat tttttccata agcttgaaca
      481 agcttggaga aattttctcc ttcatttgct tctttttttg ctttatctgc ttcttcttta
      541 gtcgaaaata gaatgatctg tgtactaact tctggataga attctttcca agcagtgtct
      601 agatctttat ctgttaattt tacattggct ttcaaaccgt attggaaagc cagcatctgt
      661 ttaaagagtt tcttatatgt ctcttctgtt tggccaactg cttttaattg ttccttgtag
      721 tttggaccga gttttttgat ttgttcatta tattcttttg ttacttcctt tgaagaaatc
      781 tcttttccgt atacatcatc aaatgctttg aagacaatca ttttttttaa tagtgtttga
      841 ttatttgatt taacttttgg gttatcataa aaatcctgaa ctgtaagtgt tccaccttta
      901 aacgttgcaa tcgtttcatt ggacttgcta caacctgaga atagtaaaat tgcacatgct
      961 gaaaggcaca taattagtag ttgttttttc aaaaaatttc ccatcctttc cttattaaaa
     1021 acagtctaac agaagaatta gggagacagt ctttcatttt cgtaaggttg ctgtagatat
     1081 tcttagttcg taaaaatcta ttctctctat gatggagaac atttacatag ttttcacgag
     1141 acaagaaaga aaagggttgt taaacttaat ataaaggagg gatgaaagat gatcaggatt
     1201 attcggttga ttggatctgt tttaatagtc ttagttggtt ttgttttagg aattcttgtc
     1261 aataatgcac ttgcggatat gtttattgga aatagtgaat ggattggagc aattgccggt
     1321 acggtcggac atttagctgt ttttttacta gctttatttc ttgctagtaa ggtcgaagga
     1381 aaaagaatag aagattatgg tgtttctgtt cgaggaaaag actgggggta tttagcggga
     1441 ggtctcgttg ttggaattgg agtgtttctg cttattacta gtccattata tttgacgggg
     1501 gcgtatcggt tagattcagg aaacgcaaac attgtgccgc tgataacgag ttttatttta
     1561 tttattgcag tgggggcatc agaagagctt ttattccgtg gcttttttca gcatcagatg
     1621 cttagatttg gtccgttaat agcaatgatt ggatctgctg cattatttgc tcttttgcac
     1681 ggattgaatc ctaacatgac atttctggca gttttcaata tctttttagc aggttgcttt
     1741 ttcagcgcac ttatctacag tacaggaagt ttatttacgg ctattggtgc tcatattact
     1801 tggaattgga cacaaggagc tattttgggt ataccagtca gcggaacgca agaacaaggt
     1861 tttttctcca ctctgatcca atcgcaaaat acaataatta ctgggggaaa ctttggggct
     1921 gaagcatcta tatcaactac attattcttg ttagtcttga ttgttggttt gttactctat
     1981 gcatataaaa caaagcggat gaagaaatac actgctgttt atcaataaaa gatgaagaaa
     2041 gcaaggctga tatcggtctt gcttttttaa gtctgtcatt gattaaacga aaaagtaaac
     2101 aaattcaagc gaatacacga catcttttgt tagattttca caactgactg tcgtgttctc
     2161 atcattttca ttttatataa ttcctttatc aaatagaagg taattatatg gagagtgata
     2221 gaaatgaaga aaacagcgac cgtattattt gttattcttg ggctgagtat aattgcagca
     2281 atgattggaa tcagtacttc tgcaaatgaa cgggcaaatg agaatctttc atttactgtg
     2341 aaaactgatc ggattgttta tgacatgaca caacaagtca ttactattcc agtcaaacca
     2401 aacaaaagcg tgaatgctag tgatgtacat gctgttctaa catatggttg ggatggtaat
     2461 ggaagttcag aaaaagttat tggggaagtt tatttaaaag acgtacaatg gaccgctggt
     2521 attgagtata caataatgat ttctgcagaa ctatcgattg atgagatcaa gagtaaagat
     2581 aaagttgatt tgattgtttt ttatgatgga cagatgacga tcacggagaa tttgaaacca
     2641 agcagttgga cagttgttgg accgccaagc agtacggata gcacaagtag tgaaagcagt
     2701 acagaaaaca cgagcggtga aagcagtaca gagagtacga gcagtgaaag tagcacagaa
     2761 aacacgagca gcgaaagcag tacggagagt acgagtagtg aaagtagcac aggaagcacg
     2821 agcagcgaaa gcagtacgga gagtacgagt agtgaaagta gcacaggaag catgagtagt
     2881 gagagtagca caggaagcac gagcagcgaa agcagcacag gaaaagtaaa taatgaactt
     2941 gatttggtac ctatcatacc aactaaggaa caaaagagaa atgcagggag caacctaaaa
     3001 agtgtgctaa aacagcctga ttttgtttct cgtggaattg atcatcaaga aaaagcgaaa
     3061 gaaacagata atagaaaaag cgatttacca gaaactggta gtagaacctt gaacaagcta
     3121 attacatgga tgggtgtgtt actcattttg attgtaggag caagctattt tcgttctctc
     3181 ttccatcgag tgaaataaag aataaaagca acagatctaa aattgaataa aacgatagat
     3241 ttttgagtga atatttcatt cgagagtgct gtattgcctg gcgaagttgt agctttgcca
     3301 gatctatttt tgtttactaa catatttgag aaacaggaaa taacaggaac acaaatggaa
     3361 aataagagca agaataattg acaatgcaca ctgcgcatgg tagtatctct gaaaaatcag
     3421 taacgaatct atttgtacgt tattcatgta gctgtcggaa taagtctaaa taatagataa
     3481 aaaggagtat gtactttgtt tgaagaattt attgatatca atgaaaggca agtatatcaa
     3541 tttttgaact attgttatga aagagacgag aaattatatg tggtgaagga tatcgcttta
     3601 gatctaaact acaccctggc taagatgaat tcggtgattc aacaagcaga atcgttttgc
     3661 gaaaggtatc cagaatataa attatctttt ctttcagaaa acaagatgat aaaagtcgag
     3721 ttttcttctc agtttttact gagtaaagtt tattctatac ttttagaggg aacgattggt
     3781 tatattttac tggattcact ttacaaaggt acttatcaat ccttagaaaa tcttagtcaa
     3841 aaaattatat aagtttgcgt acgattcaaa gaaaattaag agaacttaat actattttga
     3901 aaaactatca tttatcttgc agcttgaaaa aaggtaatcc gatagaagga gaagaatacc
     3961 gtatccgata cttttttcac ttgatgtatt ggcagtcatt cgacgaaaat cattctgata
     4021 tattatcaag tataaaaaat aaacgtcaac tattagaaaa atttgcgaaa catgcgccat
     4081 atacgcgtag tatagataga aagaaatgga tcagcctact aagaatttcg ttgcaacgaa
     4141 tgaaacaagg ccactcgatt acgtatgtcc cagaagagat gaacaaattt gtgcatccat
     4201 tgataacaaa agaagagttt acgcattaca tactagagcc tttttttgaa gcaaacttct
     4261 ggtcgatgaa tcacatcact ccaacagagc aagcttacct ttattttatg tttggtgtaa
     4321 tgaacaccta tttggaagaa gatttgttgg atactccaga taaagtacaa gaaagttggg
     4381 gctggattga atcgtctatt gtagaaaatt ttgaaaaaaa cttcaagatt tgttttacaa
     4441 cttcagaaaa aaattatcta tatgtcaatt tggcaatgat ccatttgtat tcaagggttt
     4501 ttgggacaaa aaagaaggtg gatgcttttg gaaaatcagc agaagacaga gattttcagc
     4561 atatatttcc agtgatgtat ccaatcatgc aacagttttt tagtggggta gctcgcagaa
     4621 ttccagagct taatacttat tatgaaaaga acagacggtt gatttttcaa tactgtatgt
     4681 tagtcaggga tatttttata aaacacgaac aacctgtcat attctatatt caatcaaagt
     4741 atggtaaaac acaggaatta tgggcaaaga aaaggatatt atctatgact gaacgcccaa
     4801 taaaatatag tgcatccgct gatcagttac ctgatattgt cattagcgat tatcctatcg
     4861 acaaaaaggc atatgatcaa acgaatacac agatatttta ctggaatgga caacctacaa
     4921 aaaatgattg ggcgcgtctg aaagctgaaa tcataaaaat caggaatgag aaaaatagat
     4981 ccatattaga aaaattggat ttcgttaatt aagaaaagca gattgtttat gccaaaatta
     5041 agacattgca aaaagattat tttctttaaa atcgacaaga tgaccagaaa gcaagagttc
     5101 gttgagctct tgctttcttt tagttttaga aaggaacatc ctgattttat cttcgctaaa
     5161 tccagtcaaa agttttgcag attggtcgta gataatcggt tttttcaata aagaagggga
     5221 tgtacgaatc tcaatatata gttcactcaa actgagttca tctattctgt gaacaagatt
     5281 tttataacta atagaatgag tagagagcat ttcggtcacg ccgttgtttg tcaattttag
     5341 taagtggata aattcatttt tggttagtgg ttgacgaatt aagttctgtt cgataaacgg
     5401 tatatcatgg acgataagcc aacgttttgc gcgtcttgaa gacgagcagc cagaacctgt
     5461 atacaattta atcatcatac attcctccaa tagctaagta tttatgaaat aataacatga
     5521 agaaatagtc gaaaagtgcc atttagaggt gtaaaataag aaaatgttgt cacgtataat
     5581 ggaactagct agataagaaa tactattaaa tcaagaaaaa agaaagaagg cacaagtatg
     5641 tcaaacacag ccgacgcaat agtagtgatc gatttacaaa aaggggtatg ttttgattct
     5701 tcgccgatac acgaattaga aacagtgaca gcattagtga ataaaagaat caaaacatac
     5761 gaaacgattg ggaaaccagt tattttcgtt caacacgaag atgctcaatt agtgccacaa
     5821 acaaaaccat gggagctatt gtcggaatta tctattcctg agcacagtta ttttgtgaga
     5881 aaaacccatg cgaattcatt ttataagact aatttgagtg ttttattaac tgaattggac
     5941 tgtcattcga tcgaattatg tggggcacaa gcagagtatt gtatagatac gactgtgaag
     6001 tttgctcatg gattaggata ccagcttatg atgtgtagag gagcaacgac aacttatgat
     6061 aatcctttta tgaaagcaaa ggaaacaatc gctttttacg agaaaatttg ggataaacga
     6121 tttctgacat ttttggagca ataagttgtt tcttcgttta aaaaaagcag aatggatact
     6181 ggtttatcca ttctgcttta ttacgtaaaa tgagaaaata atcctgttct atttttatgg
     6241 gataaaccat ttattttttg tataaaaaat gtttgcgttt acatataatt ctcctagaaa
     6301 cataaaaagg agtgaatagc ttgagaacaa aacaagtatt aaagggagcg ttggtcatct
     6361 tatttgctgt aagtacaagc gtcccgcaat ggacagtata tggagagagc atttcttccg
     6421 aatcggaatc tcgggaggta gaaaccacag aaactactga tgctggaaat gaaacgaaat
     6481 cgagtacttc acaaacagaa acaaatactg aaacaacggt tgagacatct gttactagca
     6541 cagatcaaga aacgaccgct accacagata accaacaaac agaagaagca acagctacaa
     6601 aaatgtcaaa cgacgaatcc tcttctgcta aagtaagtga caaagagaat gggacagaaa
     6661 atgatgatat cggtaaaggt gaaaatttca atggtaaaga atatttgaat tacttcggta
     6721 aacagatgac agaaatactt aatggaccaa cagaagacat caagtcaaaa ttaatagagt
     6781 tattagtcac tgagcttgct gttaatgatt atggtgtaga taaagagatt atcaaacatc
     6841 tgattgctac ttatctattg tacgatatcg cttttgcaaa aaacagtgtg ttgatgtcga
     6901 tgcttggagg tacagatgat gggacaacgc ctacagaaaa cacaggcttc tatccaaata
     6961 caaaacaact tcatcaaact gtactaaaca aacaagatgg caaaatgatg gatgtatatg
     7021 catattatgt cgatcaaggt tcagataaaa cagtcatgat ccatggcgga tttcgaggga
     7081 attggaataa cgggatcgtt acagaagaat acaatgattt ttataaggcc gggtataatt
     7141 tattatttgt tgattccaga gcgactggta atagcggagg cgattatgtg acctatggac
     7201 aatatgagtc agatgacgtc ctttactgga taaatcaaga agttcgagag cggccatctc
     7261 aaaagatttt actttatggt ggttcgatgg gagcagctac gatgatgtct gtattagcga
     7321 aagatattcc tgtcaatgtc aaaggaatta ttgaaaattg cggatttgca tcaattgatg
     7381 aacaactacg ttttacgtac agccagacag tagcccccgc actaccggat gccataaaaa
     7441 atcagttaga catcattggg gatcaagagc atgaagattt atttatgggc cttttgaaac
     7501 aatactattt tgatcaggag atgcatttgg atacaaaagc tgctctacct acgattggaa
     7561 tgtcagattc attgcctaaa ttgattatcc atggaacagc agatgatgta gtacctgtgt
     7621 ctaacgcgca aaaattatat gagttatcag gagggtataa agatctttta ttagtagaag
     7681 gagcagggca tggaaaggca caggaagttg accatgctgc ctacacgaaa catgtaacgg
     7741 actttttgaa agtagtgttc catgatcaga taaacgtaaa gtatgtggat gaaaacaata
     7801 aaagtctact caaagaccag gacgaaatcc agctttacgg agcttatgga gaaaattata
     7861 tgacagagca aaagacattc gaaggatacg aattagcaaa tgttgaagga ccgactgaag
     7921 ggatttttaa tgagacgata ccaactatta ttttctatta caaaaaaatc ccagtagctc
     7981 caccaaaaaa gcaagatccg acagagaatg cagacaataa gggaaagaat gaacctcgag
     8041 aggatcaatc aagtgatgat acgcaaccga ttcagatgac agtgaaagta caaaaaaaga
     8101 cagatgcaaa aaaaagaatg ttgccgaaaa ctggagaaaa gaaacattcg cttctcattt
     8161 attcaggtgg atttttatct gttagcacga ttatcacatg gatttggcga agaaagagag
     8221 ggtaaatgaa tgaaatggtt caagcgaata gtgattgcag taccagttat catcgtagtt
     8281 attttagtca ctgtattttt gattaatcaa atcacaccaa agccgattag cttgcttgtt
     8341 agaaaacaat ttgacacaag tgaaaaagaa caagtatatg cccgaccaga tgatttccag
     8401 caaaaaatcg aacaaatcga tataaaaaaa gaaatatcct atccttcaaa gtacaaaaat
     8461 aataatatgg atatctacac accgaaagtg gaaaagaaaa aattaccgat tcttttctgg
     8521 atgcatggag gcgcatatgt aggaggggat aaaaatgatt gtcgtgatta tttggaatat
     8581 ctatgttcag acacgcagca aatcatcgta aatatagatt atgaacgatc accggaagca
     8641 aaacatccta caccagttat ccaattgaac gaagccattc aagcagctaa aaaagagata
     8701 aaagatcaag cagattggtc gaatatttcg attggtggaa attcggcagg tgcacaaatt
     8761 agtggagaat atttgttagc acttcaaaat gaagaaatta aaaaagctga tcatcttgat
     8821 ccgactttgg agaacaagca gattacgaag tttatttctt tgtctggtct gttggatcct
     8881 tctgcactta ctcaagtaag tgataaaatc tctagctttc tttatgaaaa atgtggatgg
     8941 gcttactttg ataataagga ctttgaacaa tcagagaagg tcaaaagttt agccttggtc
     9001 agacatgcgg atcgttggac gcaaaacaca ttcttaacgg atggaaatac aaatacgttt
     9061 acgaaacaaa tggaagaagt aacttctgta tttgagaaag cgggggtgaa agtcacaaaa
     9121 gtagattatg cggagaaaaa tgctgttttg aaccatgaat atcagtttga tttcagcaat
     9181 aaacaagcgc aagaaacata tcaaaagtta gtaatgtttt tcaaggagta aagtcttatg
     9241 atgcaaaaga ggttgtaatc tgtcacttag ctccaagtca caaggagcaa tttcaataaa
     9301 caagcttttc atggtttatg aatattgtaa cttgtgactg aggagttaga gatggcaagg
     9361 ggcacctttt attcgaaatt tgggaatatc acacgattat tgtcacgatc tcttttacgt
     9421 aaattttata agtcagattt gttgttttga tcgtttaaaa aaagcaacaa atccgaagcc
     9481 tgcggaggca agaagggaca aaaagccgag tggtaagcaa aagaatgctg gttccactaa
     9541 tattccgttg ttattgatat aaggctggga acaaagatga atagcaaata aaatcaatgc
     9601 actagcgaaa aaaagcaaag aaatatggat agctttcata agcagcacct ccttttttat
     9661 aattgtatga taaatagatt aagaatctat aaatgtgaca agtttgttag gatgtgaata
     9721 aaatgatcat ctagcgaact tgaaacaaag atggctaatg ataagcaatt aatgattcag
     9781 caatcagata ggtacaaagt ttaccatcat acgtctagac caataataga aaatatggga
     9841 atataataga aaagatttag gataccccca aaagaatttc cccaaaaatt cgatgtatcc
     9901 taaacttttt tattttacat aacaatcata tcaactgtga aataaaatta caaatgataa
     9961 cctaaaatga ggccaaaagc cagtgttatc aaggaaatac ttaattcacc aaaatttttt
    10021 ttaatttttt agaaaataag aaacaaaaat aataaaaaaa agaaaagata aattttttac
    10081 tactgtatac taactttgtt atcgcatcta acaagtaact cgaataatac caatacaacc
    10141 ttctccaaag atgcgatgac atacgttcta acccaccgac tattgatgtt ctgtaccacc
    10201 cttgcagaac caaaaaagcc actaccaaat atttggtagt ggcttttttt gttttatgga
    10261 tttttcgctt tttctgttat ggtgctattc tttgatttca gaaatagtat gtcgaacttt
    10321 agcgatagaa cgacgtgatt ctttttcgtt gtaaaacatc aaaatcacat atagagagtc
    10381 caaaatgctt agttgagaaa tacgggaagc taaagcttca gatcggaatt ctgtttcttc
    10441 agaaatagaa ataaaaacga catcgccaag tttagcaagg gaagaattag cttggctagt
    10501 aataacgatt gtttttgctc cagtttttct tacggtttca gcaatatgga ttgtttcttt
    10561 tgattgccct gtgtgagaaa tcaaaacagc acagtctttt tccgtcaata aagaagcttc
    10621 catcaattga atatggtaat ccgtgctgtg agaagttgcg ataggggaac gcaaaaattt
    10681 atgataagcg tcagttgcta caatctctga accgccaaca ccgaaaaaat agacacgttt
    10741 agattcattg ataatagtag ccgctttttt tagatccttt tgttgtaaca acttttttgt
    10801 gtcatttaaa gttgaaatac tagactcgaa caccttttgc gccatagtga gctcagtatc
    10861 tgttttctga atattttcgt ggatagaaat agcagataga tcattttctt ggatcaacat
    10921 ggccatttta aaatctttaa atccattata accaagtttt tttgtaaatt gaaaaaaagt
    10981 agagtcagca atgcccagtt cacttgctaa attgctaata ggactatgac ttactttgct
    11041 tggattttct aaaatataat cggcgatagc ctgttctttt gggctgaagt cgttgtacat
    11101 tgttttgatt tgtagtctta tggagacgct catgagaatt cctcattttc ataataagat
    11161 tatcattttt tccgttctca ttaatgttaa agatacaagg agagtaggaa aaaatcaaac
    11221 aaaaaatatt tttgtaaaat ctatttacaa aaaaattttt ttgtataatg agggtataag
    11281 aaatggaggc gctatcatgg aaattggtgt aattggtttg ggaaaaatgg gattgaattt
    11341 agctttaaac ttaaaagagc accatcacaa agtacaagga ctcgacattt caagtacagc
    11401 tgttagtcag gcaagagaaa atgacattcc tgcttatcaa gagatctctg aatttctagc
    11461 tacttttgag cagaaaaaaa tcatctggtt aatgcttcct gctggagaga taaccgaaaa
    11521 catgttaaga aaactttttc caaaactcgg accaggagac attattattg aaggcggaaa
    11581 ctcttattat aaagattcaa tccgtcgagc agaagagttt cagaaaaatg aaatcggtta
    11641 cttggattgt gggacttctg gaggaatcga aggagcacga catcaagcct gcttaatgat
    11701 tggaggagat aaagaaactt ttctatccgt ggaacaatta ttcaaagatg tagcaattga
    11761 aagaggatat ctgtatgctg gtgcatccgg aagcggacac ttcttgaaaa tgatccataa
    11821 tggaatcgaa tatggaatga tgcaagcaat cggtgaagga ttccaacttg tacaaaaaag
    11881 tgagtatgct tttgacttgg aacaagtcgc acgtgtttgg aatcatggtt cggtgattcg
    11941 cggctggctg atggaaatca tggaagatca gttaaacgaa catcccaatt taagtaatta
    12001 tcggggaatc gtttctgcat caggtgaagc aaaatggacg gttgaaacag cgctagaaat
    12061 ggctgttcca gttccagtaa ttgcaactag tttgttcatg aggaatcttt cacaggaaga
    12121 agatagtttt tcagcaaaag tagtttcagc actaagaaat gggtttggcg gacatgaaat
    12181 agttgaaaag ggtgagtaaa atgccagatt atcatatagc aatcgttaat tccagcagtt
    12241 tcggaagagt ctttccacac cacttggaaa gattaaagtc agttggagac gttcatttct
    12301 ttactgttga tcaagagatt ccaggaaaag aactagcgga gttacttaaa ggattcaata
    12361 tcattattgc aagtgttacg ccattttttc gacgagattt ttttgaaaac aaagatgaac
    12421 tgctattgat ttcccgacat ggtattggtt acaacaatat tgatttaaaa gcagcaaaag
    12481 aacaccagac aatcgtatct atcatacctg ctttagttga acgaaatgct gtagcagaaa
    12541 ataatataac taatttgttg tccatgcttc gacgcacgac acaagcacaa gcaagagtca
    12601 aggacgatcg atgggaagat cgggcggagt tcgtcggtag gacgctgttt aacaaaacag
    12661 ttggtgtgat tggtgcagga aataccggaa gcggagtaat cgaaattttg cgtaacggtt
    12721 ttcgctgtga tgttctagct tatgatcctt acaaatcaga tttatacatc gaaagctttg
    12781 gtgcaaaaaa agtagaactt caagaattac tggaacaatc agatatcatt tgtttatgtg
    12841 cgaatttgac agaagagaac tatcacatga tttctgataa agaagtcgat cagatgaaag
    12901 aaaatgttta tctatctaat agtgcaagag gcgctctagt tgatgaagaa gcgatgatta
    12961 atggtctgaa aagcggaaag atcgcaggat atgccacaga tgttttagaa gaagaaccgg
    13021 gacgtcaaga tcatccgtat ttagcgtttg aacaagtagt gattacgcca catatatcgg
    13081 cctacacaag ggaatgttta gaagcaatgg gtgaaaaatg tgtcagagat gtcgaacaga
    13141 tcagtcagca ccttcttcct gaacgttctg tccagacaaa gagcaactat ataaaaggat
    13201 gatgaagaaa atggtgaacg gtttaaaagt gaaaacaggc cctcaatttt atttatatga
    13261 agaaggtggt atttcaaaag tttctgatct cttgaaatca tacggcgcga aaagagtact
    13321 ggtcacccac ggaacggtat catgggaaaa ggcgctacct aaattagtat ttttgaatga
    13381 cgaaacgatt caattttttt atcatcgtta ttctggagaa tgtagttatg ctgaagccag
    13441 acgaatagct acaatcatca aaaaaatgaa atcgatttcg tagttggcgt tggcggagga
    13501 aaactgaccg atctagtggg ttatagtgcc aatcttgcaa gaatcgattt cggagtcatc
    13561 cctactttag ctagtaattg tgctccatgg acacctcttg ctgtcatgta taaggagaat
    13621 ggacaagcag aagggaaaac agaacacttt tacagacaag ctgctttttt tataacagat
    13681 ccccaattag tgattgattc ccctgttcca tatttcattg ccgggttagc agatacacta
    13741 gcaaaatggt atgaatctga gctcatctta gaacaagatt ttttgcaaga tgaatcattt
    13801 ttgaagctag cacaagatac tgcgaagatc tgcaaagaag agattttgaa tcattctgct
    13861 aaagcaatcc aagatatggc acaaagaaaa ctgacatcgg agttcaaaca gctttcagag
    13921 attatctttg ctgttgctgg tttagttgga ggattaggag ataaatacgc aagaaatgcg
    13981 gcagcacatg ctatgcatgt cgctatcagt aaatatattc cggaaagcca tcgttttcta
    14041 catggagaaa aagtagcgta tgggattttc tatcaactgg cactagaagg tcgctgggaa
    14101 aaaatagacg agttatatcc tttgtatcgg gaattagaac tgccggtttc tctagaacaa
    14161 atgggattat ttccgaaaga taaacagata atagataaaa tggctgaatt tattgattcg
    14221 aaagagaaag tgcatttgat tcctatagag gtaacacaga aatcactgat acagtcaatg
    14281 atggaactgg aaggttatat cagaaagatc tgaaggagga ggacacatgg aaacattaag
    14341 tgtagtacaa agtttgttga ttgctttatg ggtagccgcg gttatgtccc gctggttagg
    14401 tggaggagcc acgttgacct tacgtttttc tcctttgatg actggattag tcgtcgggtt
    14461 gatcatgggg gaagttgcac aagcgatgat tgttacagcc gctttgcaaa tgatttatat
    14521 gggggttttt tctccaggag gatcaatgcc agctgaacca tctattgcag cagctatcgc
    14581 agtaccagtc gcattattag gggatctgtc gccagaagca gcgattgccg ttgcggtgcc
    14641 agtcggattg ctcggaagtt acttgtatca gtttcgtttt ttcatcaata cttttttagg
    14701 taaatataca gacaaagctg tagaagattt gaatacaaaa gctatggccc gatcaattat
    14761 tctttatcca acggttgcct catttttatt attcgttccg cttgttttca ttgctttata
    14821 tttaggggcg ccattgatcg ctgatgtaat caaagcttta gagggaacag tcgttatcca
    14881 cattcttgaa gtagtaggtg gagggttagc ggcaatcggt atcgcgacaa ctgtatacgt
    14941 tatcggtcga aaagattatc tggtcttttt cttccttgct tattttatga gtgttgtttt
    15001 gaaatcttta gatattacga tggttactta tgccattttt gggattatta tcgctttgat
    15061 ctttgtccag tcgcaaaaag ggaaacaagt acagcctgcc acaagcaata acaattcagc
    15121 atcttttgat gacgaagatg actatgatga cggattttaa tttaggagga atgggaaatg
    15181 gctgaaataa tggataatca aacaggtgct cctgaagaaa tcacgaaaaa agatgtaaca
    15241 aaagcctatc tgagatggca ttttgcaaac gaaattcctc actcttttga acgttacttg
    15301 gcgccatcac tactatacgg aatgatgcca atcttgaaaa aactttataa agatgaagac
    15361 tctttaaaag cagcatataa acggcagtta ctgttcttca acactcaatt aagctggggt
    15421 ggcggtgtga ttactggcct aatgtcttct atggaacagc aaagagcaga agaagagtat
    15481 gaagaaaaag agatcttgat gcaggatgac ttgatgtata acacgaaagc gggattgatg
    15541 ggggcactag caggaatcgg agatgcaatc gattcaggaa ccgtccaata tatttttatc
    15601 gcaatcgcgg taccttgggc acaacaggga agtcctttag gcgcactttt tccttttatc
    15661 tgctttgggt tgtatcaggt tttactaggg gtatttttcg caagacaagc tttcactatg
    15721 ggccgtaatg ctacaggcct aatgcaaaac actggtgttc agacagcgat tgaaatgcta
    15781 tcagttttag ggttgtttat gatgggggtg cttgcgggaa actatgtaaa agtagaatct
    15841 tcattgaaat ttgcgatatc cggacgtgag tttgttatcc aggaaattct tgatcaaatc
    15901 gtgccaggga tattaccttt agctgtcgta atgggtgtgt acttgttcta tacgaaaaaa
    15961 ggattgaaag tcacacaagc attattatgg ctgactggaa tattaattgt gctagcagga
    16021 atcggtattt tataaaaaga agggaacgtg aataagatgg gtgtagtcaa tttagcgcgt
    16081 gtggatgagc gtttgatcca tgggcaagta atggtaacgt tatctcaaaa aagtggagtg
    16141 aattccattt ttgttgtaga cgaagttgta gcaaaagata aatttatgag agatctttac
    16201 aaaaatgcag gaagccgtac aggacaaaaa acaattgtca ttacaccaga gaaagctaaa
    16261 ttctattggg atgaatatca atttaaagaa tacaattgta ttttgatcgc aaaaacagtc
    16321 caaacgattt acgacttagt aaaacacgga atccccatgg aagaattgaa tattggaggg
    16381 attgctcaaa aaaatcctga tacagatctt ttagtgacga aatctgttta tttaaataaa
    16441 tcagatgctg aaaaattgaa agaaatgaat gaacaatata gcgtaaagaa tatttatttt
    16501 caagccactc catctgcacc taaaacagga ttaaaagatg tattgaacaa gtttgatttg
    16561 tcataaacaa atgcttagaa aaaagataaa gagggcgaaa aaatgaagaa gaacgagctg
    16621 tttcgtgatt gggaatttcg ttatcgctat atttaccgta agcgccgtac aaaaaagagt
    16681 aaacagcgtt ttctttcagc attagtatca gatatttaca gtatgagaac agatgtgaca
    16741 gtcattgctt acgatacgct agcctaccga tcaaaaaata tttatgttgg agatatcgaa
    16801 aaagcagaga aagttatctg tacttattac gatacacccg ttcatgcatt aggaagttat
    16861 ttcatgtttg attggaaaga ccaaaggaaa aaaacaatct actctatatt attgtccttc
    16921 attttgttgt tcagtttagg ttggtgggga atgatgatat ataacaaaaa tcctcatcac
    16981 gtattcgatc tgctatccgt ataaacaggt atcactgtac tagcatttgg gagctacttt
    17041 ttcttattgg gaaaagcagc tcgcggatgg cctagcagac aaacttttat tcgaaatact
    17101 tcatccatat taacgatgct agaaatgatc cgtacgattg atgatccgag tgtagcgtat
    17161 gcgtttgttg atgaaggctg ttatggagaa aagggcttag acagcgtgag atcgggtatg
    17221 aaaaaagagg ctattctttt ttatctagac tctgttgggg cagatacacc gctacagttt
    17281 tcgggaaatt atttttcaaa taaagaacag tggctgaaac aagttgataa gttgaaagaa
    17341 aaaaacgtca attatatttt tagtgcaaga aaaaagcaag cgcagttctt ctatttgacc
    17401 aaaacagatt tacgagggaa aacatttaat tggcaaaatg ccaatcagat tattgctctt
    17461 tttcgataag taaaaagtgc aaaacaagga gtgagaagac taatgttgga aatcatgatc
    17521 gttacacatg gtaagttgag cgaaggactg aaagatgcgg cggaagtaat tgtcgggaca
    17581 tcgagccata ttgaaacaat gtgcttagag tctggggagg acatccaaac attcggagag
    17641 aaggtcaaag aaaaattaac tatgatcaac acagcagaac ccatgctagt atttgttgat
    17701 ctaattagcg caagtccgta caatcaagcg ctcttggcag tcaatgaact gactgaagaa
    17761 aagaaagcag ccacttatgt catagggggc gtgaatctgc caatgttgtt agaagcaatc
    17821 aatcatcaga tgcttcacac accgatagaa gaagcggtgg aacaaattgt caaacaagga
    17881 aaagataatt taactgtttg gcatgcttca gcggaagaaa aaaaattaga tgaggacgat
    17941 ttttagaagg aaggctgaaa agatgaagtg gggatttaga tggtatggtg aaaaagggga
    18001 ttcgatccca ttaaaatatg ttcgccaaat accgggaata aatgggataa ttggaacttt
    18061 gctgactaaa atgcctggag atgtttggga gatttcagaa attgaggagt tgaaagcatc
    18121 tgttgagagc caaggactgg aactgcttgg aattgaaagt gtagcgatcc acgatgcaat
    18181 caaggcaggg acagatgaac gagattacta tattgataac tatatacgaa caatcaaaaa
    18241 cttgtctcga tgtggggttc gattgatttg ttatagcttc aaaccaattt ttggatgggc
    18301 gaaaactgat ttgtttaatg aaaacaacga tggaagctat tctcttttat acgatcagaa
    18361 aatcgtcgat cagatggagc caggagaaat gtatcaattg atacacagcc agtcaaaggg
    18421 attcaaattg ccgggttggg aagaagaacg gttgaaaaaa ttcgctcagc tggaaaaact
    18481 gtatgaagga atgaccgaag aagaattatt tgaaaatctg acttattttt tagaaaaagt
    18541 gattccagtg tgcgaaaaag cagatgtaaa aatggctatc catccagatg atccgccatg
    18601 ggaacttttt ggttttccta gagtgacgaa aaatcttggc gacttgaaga aaattctaaa
    18661 tgcagtagat tctccatata atggactaac gctgtgcaca ggatctttag gggcaaatcc
    18721 ggaaaacgat ttagttgaaa tcatccatga ggtaggttcg cgcatccatt ttgtccactt
    18781 cagaaacgtg gagtttttag gagaaagaaa gttcaaagaa acggcacatc cctctacaga
    18841 aggctccctt gatatgtatc atattatgaa agcgttggtg caagaagggt tcgacggtcc
    18901 tatacgccct gatcatggta gaaccgtgtg gggtgaagtg gctatgcctg gatatggcct
    18961 gtatgatcgt gcaatgggac tcacttacat gcaaggtcta tatgaggcga taagtaagga
    19021 aaaaccactg ggagagcatt atgaataaaa tcaagctctt catctttgat atggatggcc
    19081 ttttattaga aactggacgt ttagcttatc gtgcttatgt aaaatcagca caaaagtatg
    19141 attatgaaat gcgtaaggaa gtctattatt tactaactgg ccaaacagaa atggcgataa
    19201 gagaacaaat gggcattttg tacggagaag atgttccata tatcaaatgg cgagaagcaa
    19261 tcaatcaata taaagaaaaa atcgtgaaag aagataagag agtatatacg aaaaaaggag
    19321 cagaagaaat tttatctttt gcaaaagaaa gaggaatcca tacaatcgtg gcttcgtcta
    19381 atacacgggt aaaagtagaa atgtatttaa aaatggaaaa tctatatgcc ttgtttgatc
    19441 aaatcatttc aggggatgat gtaaaaaaag gaaagccgga accagagatc tttttgaaag
    19501 cttgttcaaa aatgaatatt cctccaagtg aagcacttgt atttgaagac tcgattgcag
    19561 ggatcgaagc agcaaggcga gcgggcattt tgtcttttct catcaaagat catttgatag
    19621 ggttgcccga tcataatggg aagcataagt tgaaagtaga tgtaagttta tatgaatcca
    19681 ataaagcaga ctatgaattt tatgacctgc atcaagcaaa acgattttta gaaagtaaag
    19741 ggttgtatct ctaataaaga ggactatttt ctagtcctct ttatttattg tcctgcacga
    19801 ctgagggaac agatgaaaaa gaagtaattt tttattttga agtatctacc ttatttgtag
    19861 tgaacaattt agaacaaaca caaaacgcat ctactgttat ctaccagtag atgcgtttaa
    19921 agagaagaac gagaatcgtc catatgaggg tttccactcc ttatgcagat ttttttaatt
    19981 cttgcactgc ttgttcgatc gtaacacctg tcgctacatg attggtattt tctttgtcaa
    20041 aaacgacaaa tagattcaaa tctgtatcca aggttactcg atattgtata tctttgttat
    20101 agaatgtcat agccattctc ctttctttaa atacttgctg aaaggcaacg aacgcgttga
    20161 taacgaaatt ccatttataa gtataccacc aattgaaagg ttttcatagt taagaaattt
    20221 taaagtcaaa agttgcagat aaaagcaaat aaacaagaaa acaaaaagtg aatcaatcgc
    20281 ggttattcaa ctttttcttc gattcgaaga caatagaaga caatgaattt tatatttcat
    20341 tgcattatcc ttgtgttatt agaaaaagca tgatatatta aatgagtaat ctattttgaa
    20401 acgtaatttg agcgatatat ctacatagat aaaactcctt ttgcctatca aatatttatt
    20461 gcaacgaaaa aacgtacatt gatacgtatc aggaggaatt tatttatgaa taacggtaca
    20521 gtaaaatggt tcaacgcaga caaaggtttt ggctttatca ctggtgaaga tggaaatgat
    20581 gtatttgcac atttttcagc tatccaaggt gacggcttca aaactttaga agaaggccaa
    20641 gcagtgtctt acgatgttga agatggtcaa cgtggccctc aagcaacaaa cattgttaaa
    20701 gcttaataaa aattgacttt taaccgtctt ttcgaaagaa aagacggttt ttttgtacga
    20761 aaaaattcat tggtcgttga atgggctgaa tagtagtgta taaaacaatt ttgtggagcg
    20821 tgaagctttt atggtagagg aaaatacgaa taaagtgatg ttgataaaaa gattcctatc
    20881 ttgaatatga atcgagatgt cttgaaaatg aatgagtttg accaatcatt atcctatcga
    20941 aatttacagt ttcgttcgat tggtctgtca attaatgaaa aagtaaaaaa gaatatgttc
    21001 taggaagagg gcggcacatc taaattttta agtaacggac agttcaaata tcaagcattg
    21061 aaagaggcta tcctactgtt tgaaggaata cgaatatata aaaacgacaa agaggaaaaa
    21121 gtagttcatt aaatcccttc aagtgatgaa agaatgagat ctaataaaac ctgatctttc
    21181 atctgttttt ttaataacgt aataattttt ttattttttg t
//
LOCUS       DAGCWU010000030        21132 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.030, whole genome shotgun sequence.
ACCESSION   DAGCWU010000030 DAGCWU010000000
VERSION     DAGCWU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.030"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(44..1276)
                     /locus_tag="MBZ71_000908"
     CDS             complement(44..1276)
                     /locus_tag="MBZ71_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBT4428879.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1530..2099
                     /locus_tag="MBZ71_000909"
     CDS             1530..2099
                     /locus_tag="MBZ71_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4428880.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2277..2717)
                     /locus_tag="MBZ71_000910"
     CDS             complement(2277..2717)
                     /locus_tag="MBZ71_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBT4428881.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2875..3639
                     /gene="map"
                     /locus_tag="MBZ71_000911"
     CDS             2875..3639
                     /gene="map"
                     /locus_tag="MBZ71_000911"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBT4428882.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3671..4594
                     /locus_tag="MBZ71_000912"
     CDS             3671..4594
                     /locus_tag="MBZ71_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBT4428883.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4670..5809
                     /locus_tag="MBZ71_000913"
     CDS             4670..5809
                     /locus_tag="MBZ71_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBT4428884.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5802..6602
                     /locus_tag="MBZ71_000914"
     CDS             5802..6602
                     /locus_tag="MBZ71_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4428885.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6602..7429
                     /locus_tag="MBZ71_000915"
     CDS             6602..7429
                     /locus_tag="MBZ71_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4428886.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7407..8141
                     /locus_tag="MBZ71_000916"
     CDS             7407..8141
                     /locus_tag="MBZ71_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4428887.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8241..9107
                     /gene="rfbA"
                     /locus_tag="MBZ71_000917"
     CDS             8241..9107
                     /gene="rfbA"
                     /locus_tag="MBZ71_000917"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBT4428888.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9121..9693
                     /gene="rfbC"
                     /locus_tag="MBZ71_000918"
     CDS             9121..9693
                     /gene="rfbC"
                     /locus_tag="MBZ71_000918"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBT4428889.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9715..10743
                     /gene="rfbB"
                     /locus_tag="MBZ71_000919"
     CDS             9715..10743
                     /gene="rfbB"
                     /locus_tag="MBZ71_000919"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBT4428890.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10841..11692
                     /gene="rfbD"
                     /locus_tag="MBZ71_000920"
     CDS             10841..11692
                     /gene="rfbD"
                     /locus_tag="MBZ71_000920"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBT4428891.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11727..13760
                     /locus_tag="MBZ71_000921"
     CDS             11727..13760
                     /locus_tag="MBZ71_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428892.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13804..15084
                     /locus_tag="MBZ71_000922"
     CDS             13804..15084
                     /locus_tag="MBZ71_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBT4428893.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15294..16100
                     /locus_tag="MBZ71_000923"
     CDS             15294..16100
                     /locus_tag="MBZ71_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4428894.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16112..17332
                     /locus_tag="MBZ71_000924"
     CDS             16112..17332
                     /locus_tag="MBZ71_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4428895.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17322..18908
                     /locus_tag="MBZ71_000925"
     CDS             17322..18908
                     /locus_tag="MBZ71_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4428896.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18947..21085
                     /locus_tag="MBZ71_000926"
     CDS             18947..21085
                     /locus_tag="MBZ71_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4428897.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6631 a         3590 c         4418 g         6493 t
ORIGIN      
        1 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
       61 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      121 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      181 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      241 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      301 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      361 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      421 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      481 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      541 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      601 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      661 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      721 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      781 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      841 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      901 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
      961 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1021 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1081 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1141 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1201 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1261 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1321 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1381 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1441 aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa attaatcaat ggtggaagcg
     1501 ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa aaagacgatt actagagaac
     1561 agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga tttactgcga
     1621 gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag tttaaaaata
     1681 tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa tatgaagtgt
     1741 tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat atccattttg
     1801 caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt ggaggaaaag
     1861 agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat ccagcttatg
     1921 tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc atagctactg
     1981 ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag attgaaaaga
     2041 tgatccaaga cagtatagat gctatcttga aattcaaaga accaatcgaa atcgattgaa
     2101 tatactcaaa agttccacaa atcagtaggt aacaagagat gtgtcaaaat aaggaaaagt
     2161 tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat gaatgcaatg
     2221 aataaaagag ccatacagat tgaataaatc tggatggctc ttttattgca agtatattaa
     2281 atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc ttcttcagca
     2341 ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc aaaagcagca
     2401 tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga tccgcagaca
     2461 ccgtaaattt ttcccaatag atccaattct tgcaagtctt cataaaagtc tacgatctca
     2521 tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc tgcctcctcg
     2581 aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc cagattttca
     2641 aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct agcatatacg
     2701 atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat tatgaattat
     2761 accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg tcatttggct
     2821 aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga aaatatgatt
     2881 actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt attagcagat
     2941 gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga tattgaggta
     3001 tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg ctttgaaggg
     3061 tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt ccctcgtaaa
     3121 aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt gaaaggcggc
     3181 atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat cgaccgattg
     3241 atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt agggaatcgg
     3301 attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct agcgatcgtt
     3361 cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc agttcctcat
     3421 tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac gatcgaacct
     3481 atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac tgcgtataca
     3541 agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa agatggtcca
     3601 agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc aataggtacg
     3661 ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga tgcgctttat
     3721 tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg tcgtcgttgc
     3781 ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata ttttaccgtt
     3841 tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc ctgagaccgt
     3901 ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag gaggcttgtt
     3961 atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg ctttgcaaat
     4021 tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg tccgtttctt
     4081 ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta tcgttcttgg
     4141 gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg tgaactttat
     4201 cgatcagttc caaactttga agtggccaat cacattggta gctttatttg tcatcatgtt
     4261 cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca ttccgggcac
     4321 gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct atgctaggta
     4381 ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac tcatgctttg
     4441 gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag ttgttcaaga
     4501 atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat ggttcaataa
     4561 attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat tgtaaattta
     4621 tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc
     4681 ttttagtatg gttataagat tttttctgac aattttatta tcactgatat taacacctgt
     4741 attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa
     4801 taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac
     4861 tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc
     4921 tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa
     4981 aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga
     5041 ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac
     5101 gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct
     5161 ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat attttttcct
     5221 ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg
     5281 tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt
     5341 cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc
     5401 atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat
     5461 taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg
     5521 actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt
     5581 gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt
     5641 gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga
     5701 gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca
     5761 agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc
     5821 agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata
     5881 attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt
     5941 cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct
     6001 attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg
     6061 attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag
     6121 cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta
     6181 atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt
     6241 tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg
     6301 aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta
     6361 tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt
     6421 actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca
     6481 caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt
     6541 ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct
     6601 gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc
     6661 agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga
     6721 taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca
     6781 tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga
     6841 aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga
     6901 actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc
     6961 tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc
     7021 agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg
     7081 gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt
     7141 ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt
     7201 acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg
     7261 ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag
     7321 tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca
     7381 tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt
     7441 cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt
     7501 tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata
     7561 catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc
     7621 ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa
     7681 tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact
     7741 attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg
     7801 agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta
     7861 ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac
     7921 ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg
     7981 gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa
     8041 acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc
     8101 tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct
     8161 taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc
     8221 aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc
     8281 tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga
     8341 tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa
     8401 cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc
     8461 agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag
     8521 aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg
     8581 gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg
     8641 gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag
     8701 ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt
     8761 atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag
     8821 aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac
     8881 ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat
     8941 ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa
     9001 ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga
     9061 aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag
     9121 atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc
     9181 gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg
     9241 aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg
     9301 cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca
     9361 gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga
     9421 tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga
     9481 ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca
     9541 gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat
     9601 ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa
     9661 gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa
     9721 aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta
     9781 aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa
     9841 gagaacttgg ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat
     9901 gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa
     9961 tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt
    10021 acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact
    10081 gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa
    10141 ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa
    10201 gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt
    10261 tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa
    10321 gtaacgagta tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt
    10381 gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga
    10441 attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa
    10501 ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa cgaccgcgca
    10561 gggcacgatt tgcgttatgc aatcgattcg acaaaattgc gagaagagct aggttggcag
    10621 cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat
    10681 gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa
    10741 taatacagag gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct
    10801 gtctcttttt taaaataaaa atcaaggagc ggataaccat atgtttttat taactggagg
    10861 aaatggacaa ttaggtacag aattgcgcca tttattagat gaaaaaaatc tgccatatgt
    10921 ttcaacagat gcaaaagact tggatatcac agatgctgag aaaacaatgg cttatattac
    10981 agaattaaaa cccgatgtta ttttccattg cgcagcttac acagcagtag ataaagcaga
    11041 agaagaagca aaagaattag atgagaaaat caatgtcgat ggcacaagaa atgtagcact
    11101 tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt acagactatg ttttcgatgg
    11161 aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg
    11221 tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat
    11281 acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct
    11341 agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac
    11401 acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta
    11461 tcatttgtcg aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa tattgaaaga
    11521 tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc
    11581 gcagtattca gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca taccaacttg
    11641 gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt
    11701 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11761 aaaagccaaa agcgaatcag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11821 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11881 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    11941 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12001 tctcacaagc tgaagaggac ttttcgttgg tgaacgagga ttattcatta aacggacaaa
    12061 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12121 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12181 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag aggaaaaaag
    12241 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12301 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgattgct ttttatcatt
    12361 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12421 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12481 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12541 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12601 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12661 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12721 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12781 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12841 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12901 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    12961 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13021 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13081 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13141 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13201 tttacctcgc ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13261 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13321 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13381 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13441 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13501 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13561 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13621 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13681 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13741 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt agtagaaagg
    13801 agagtgaaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13861 ttgcttttgt tgctgacgct agtctttttt gtttggacag caggggtagg ttttccagca
    13921 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    13981 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14041 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14101 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14161 gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt atatcgtgta
    14221 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14281 gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc tttattgatc
    14341 agtggatttg ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg gatagcagtc
    14401 gttgcagcct ttttaggtat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14461 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14521 attgcctaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14581 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14641 ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca gcgaggaaac
    14701 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14761 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14821 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14881 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    14941 gccgttcatc gatatgatga accagaagtt acggaggaat tctatgttat ggaagaggca
    15001 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15061 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15121 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15181 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15241 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15301 aagatttgta tttatttatg aaggacattt atcagaacag aaaattactg ctacagtttt
    15361 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15421 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15481 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15541 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15601 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15661 acacccattt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15721 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15781 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15841 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15901 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    15961 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16021 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16081 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16141 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16201 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16261 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16321 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16381 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16441 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16501 tatcattgaa tttgctgaca tcggcgacca tatttatcaa cctgtaaaaa cctattcaag
    16561 tgggatgttc gttcgtctag cttttgctgt agcgatcaat gtgaatcctg atattttgat
    16621 tgtggatgaa gcattggcag taggagatct tgaatttcaa ctaaaatgta tggaaaaatt
    16681 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata ttagttcgat
    16741 tcgccgtttt tgtgatcgtt cctattggtt gaaaaatgga aaagttgtcg aatatggtga
    16801 tacgatggaa gtaacgacga attacgaaaa ctttttgaag aaaaaatcag ttaaaacagt
    16861 agatcgaaat aaaaaccaag tagaacgctt cgcagctcca gatatcgtag acgtgacgaa
    16921 agccgaattg ttggataaag ataaaaatcc agtggatatg ctggaacaag gagaaaaact
    16981 atacgttaaa gtaacctatg aagtaaaaga cgatacaatc aaaaatccag ttttaggaat
    17041 tgctctgcgt acagtcgaca atttttatgt gtgcggactc aatactctac tagatggtat
    17101 caagatccct tggaaaaaag gtgagaatat cttttatctg gagtatgaaa agttaggttt
    17161 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17221 ggtatacaag acaaaataca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17281 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17341 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17401 aagttttaga gtttggtcct ggaaacggcc gattaacgaa acatttgatc ggagcaaaac
    17461 aatgtgaagt gagcattgtc gagctagata aggaattgtt cgattttgtc agcgagtttg
    17521 cccaagatgg attttatgga gatatcgaaa gttttgaatg ggcaaactat tatgctggac
    17581 aaacatttga ttacgttttg tttgccgatg ttttagaaca tttggtagat cctggaaaaa
    17641 cgttgaagaa agttagagaa tttttgaatg aagaaggaga aattctaatt acattcccta
    17701 accttgcgca taattctgtc atgattgatt tgttcaataa tcaattacca tgggcttctt
    17761 atggtttgct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17821 ttgaaaaagc aggattatac atcaatattg aagattattt gtacttagct gtaggtgata
    17881 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgttatgat ttcaaaatgc
    17941 gaccatttgg cgaagtctac caatacttct tctcattgat gaaacatcca gtagcacaaa
    18001 gttccattgc gcagccgcaa aattctaatt atgtgaaagt attagaagta atccagcaaa
    18061 cgaaacaaga tgaaataatt caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18121 cactaagttt ccatgtatca gaattagcag aacgaatggt gtttcgcttc gcgcagcaac
    18181 ctagctttat cgaattcagc gcagaagctg ctggagaaaa actcacattt atcgattcaa
    18241 atgctgtagt caaaacagta aatgattgtt atttgtttga tggtaaagat ttaccagagt
    18301 tctttttaac ggatattgca gggaaagaag tgactatcca ttgtcactat cgttttatcg
    18361 gagaattgac accaacaatg aaagaattgc tggaaacaat ccgtccaatg gctgaagtta
    18421 caaagcagct ttctgagaaa aatgatgaat tagaaagaga aaatcattat cttattgaga
    18481 ataatacaag attagatcag acgttgaaaa caaccataaa tcgctattgc acgttgttgg
    18541 aaagtgatga atggacaatc aaacatagat tagggcgacg taagaaagaa acatcgaaaa
    18601 aaatccaaga aaaagagctt tctctatgta ttgacgaaaa aatctgggat gcagaaacaa
    18661 agatattgaa aatcatcggg tggggaatcg ctaattcgga tcgccaacca ttgtcctata
    18721 agttatcagc cgaccaatca ccattttttg aagcaattcc tgttttacga gaagaagtca
    18781 atcaagcaaa acagctagct gaaggagcaa aagcaggatt tgaactgcgg atcctttgcg
    18841 agaaggaacg ttcattctta gttgaagcag tggctcaaaa tggtcaatca tggatcattg
    18901 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    18961 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19021 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19081 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19141 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagcaat
    19201 cttagacttc caaacagcaa gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19261 tccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19321 gataaattat gcaaggactc atggcgtaaa aaaagcaatc cgccgggtca aactggaatt
    19381 gaaaccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac ctgatctgaa
    19441 agagcaaagg aaagaagtac aaggatttgc ctaccgccca ttgatttcca tacttatgcc
    19501 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac ttgatcaaac
    19561 gtatgatcac tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19621 gtgtctagaa tcctatcaag caaaagatga tagaattaaa gtcgttttcc gacaagaaaa
    19681 tggacacatc agtctggcaa ctaattctgc attgaaaatg gcagaaggag agtttatagc
    19741 gctcttagat aatgatgacg aattacctcc atttgcctta tatgaagtgg caaaagtgct
    19801 aaacgtacat ccagaactgg atttgatcta cagtgacgaa gataaaatcg atgcagatgg
    19861 gaatcgcttt gacccgcact tcaaagcaga ctggtctccc gatacattga tgggaaataa
    19921 ttatatctct cacttaggcg tatatcggac aagcattgtt aaggaacttg gcggtttccg
    19981 taaaggatat gaaggctctc aagattatga tttggttctt cgtgtgacag aacaaattcc
    20041 tgcagaacac atttaccaca ttgaccgtgt cttgtatcat tggcggacga tcccgggatc
    20101 aactgcaagc aatggagaag ctaaaagcta catctatgat tctggagtga aagcattgac
    20161 agatgctcta tctagaagaa atatcaaggg aagcgtacat cctggcagaa tttctggttt
    20221 ttatgaaatc acttatgatg ttttgcaaga agacttggtc agtgtgatca ttccaactaa
    20281 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttatcc
    20341 aaattatgaa atcattattg cagataatgg aagtacagat cctaaaatgc aagaattatt
    20401 tgcagaatac aagcatcagc tgaaagaccg atttatcgtc gaattaattg acattccatt
    20461 caattattca cgtatcaaca atctggcagc agaaaaagca aacggcaaat actttttatt
    20521 cttgaataac gatacagaag tgattgaacc tgactggatg acagcgatgg tttcttatgc
    20581 acagtttgat cgaatcggct gtgtaggagc aaaattattt tatccggatg atacaacgca
    20641 gcatgcaggt gtattattag ggatcggtgg cgttgcagga catgcattga ataactatga
    20701 tcgaacacat tgtggttact ttggccgatt ggtcattgac gtcaattact tagcagtcac
    20761 tgcagcttgt atgatggtca aggcagctga cttcaatgct gtcaatggat ttgatgaaat
    20821 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20881 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    20941 agatacacca gaaaaacaaa gacgttttgc tggggaaatc aaaaaaatgc aagataagtg
    21001 gccttcttat attgctcatg acccatttta taacgataat ctaactaaag aaggaatcgg
    21061 cgatttttcg ttgagacctg attgattaaa agaactgtat tttcattttt attaatgaaa
    21121 atacagttct tt
//
LOCUS       DAGCWU010000031        20972 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.031, whole genome shotgun sequence.
ACCESSION   DAGCWU010000031 DAGCWU010000000
VERSION     DAGCWU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.031"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(215..1522)
                     /locus_tag="MBZ71_000927"
     CDS             complement(215..1522)
                     /locus_tag="MBZ71_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBT4428898.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1525..2106)
                     /locus_tag="MBZ71_000928"
     CDS             complement(1525..2106)
                     /locus_tag="MBZ71_000928"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBT4428899.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2222..2319)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2678..3970)
                     /locus_tag="MBZ71_000929"
     CDS             complement(2678..3970)
                     /locus_tag="MBZ71_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4428900.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(3967..4590)
                     /locus_tag="MBZ71_000930"
     CDS             complement(3967..4590)
                     /locus_tag="MBZ71_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428901.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4593..5429)
                     /locus_tag="MBZ71_000931"
     CDS             complement(4593..5429)
                     /locus_tag="MBZ71_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4428902.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5446..6333)
                     /locus_tag="MBZ71_000932"
     CDS             complement(5446..6333)
                     /locus_tag="MBZ71_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4428903.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6349..7632)
                     /locus_tag="MBZ71_000933"
     CDS             complement(6349..7632)
                     /locus_tag="MBZ71_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4428904.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8315..8998)
                     /locus_tag="MBZ71_000934"
     CDS             complement(8315..8998)
                     /locus_tag="MBZ71_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBT4428905.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(9016..9633)
                     /locus_tag="MBZ71_000935"
     CDS             complement(9016..9633)
                     /locus_tag="MBZ71_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBT4428906.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9643..10467)
                     /locus_tag="MBZ71_000936"
     CDS             complement(9643..10467)
                     /locus_tag="MBZ71_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBT4428907.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10460..11020)
                     /locus_tag="MBZ71_000937"
     CDS             complement(10460..11020)
                     /locus_tag="MBZ71_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428908.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11193..12149
                     /locus_tag="MBZ71_000938"
     CDS             11193..12149
                     /locus_tag="MBZ71_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4428909.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(12454..13410)
                     /locus_tag="MBZ71_000939"
     CDS             complement(12454..13410)
                     /locus_tag="MBZ71_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBT4428910.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            13514..14116
                     /locus_tag="MBZ71_000940"
     CDS             13514..14116
                     /locus_tag="MBZ71_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4428911.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(14204..15703)
                     /locus_tag="MBZ71_000941"
     CDS             complement(14204..15703)
                     /locus_tag="MBZ71_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBT4428912.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(15719..16243)
                     /locus_tag="MBZ71_000942"
     CDS             complement(15719..16243)
                     /locus_tag="MBZ71_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBT4428913.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            16541..17290
                     /locus_tag="MBZ71_000943"
     CDS             16541..17290
                     /locus_tag="MBZ71_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBT4428914.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            17281..18066
                     /locus_tag="MBZ71_000944"
     CDS             17281..18066
                     /locus_tag="MBZ71_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBT4428915.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            18035..18751
                     /locus_tag="MBZ71_000945"
     CDS             18035..18751
                     /locus_tag="MBZ71_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4428916.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(18853..19887)
                     /locus_tag="MBZ71_000946"
     CDS             complement(18853..19887)
                     /locus_tag="MBZ71_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBT4428917.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(19891..20949)
                     /locus_tag="MBZ71_000947"
     CDS             complement(19891..20949)
                     /locus_tag="MBZ71_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBT4428918.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         6297 a         4465 c         3655 g         6555 t
ORIGIN      
        1 caatattcaa caaacatgag aagctttttt ttgaaattgt tccttgtgac tcggagatga
       61 acggcaggct cacgacctct tccttcaacg gtgttcaatc agctgaccct cggtattagc
      121 tcaaacaaac ggcaaaatac gaggaactat ttttccgttt gtttctctaa caatcaggtc
      181 aaaacgctga tttcacaacc tttcttttgc tctattactt cttgaaggct tcttccaata
      241 tttcttcatc ttttttcaat ccattaaaga tcaaattcaa tatgatagcc gtaatgctac
      301 tcattacgat gccatttcca gtgaacattt gcaatgtttg cggcatttga ctgaataatg
      361 tcggcgtaac attgaaaccc agcccaaatc caacagaaac agcaacaatc aatagatttt
      421 tatcattgct gaaatcaact tccaacagca tacggattcc ttggactgcc accatcccga
      481 acatgactaa cattcctcca ccaagtaccg gctcagggat gatttgtgct aacgcaccga
      541 ttttagggaa taatcctaaa acaatcaaga agaatgcaga gaaatagatg ggttttcttg
      601 ttttgattcc cgaaagttga accaatccga cattttgaga aaagccagta taagggaatg
      661 tattgaaaat accgcctaag ataacggcaa gtccttctgc acgatatccc tttttcaatt
      721 cttcttctcc aacatttttc ccagtaatgt caccaagtgc aaaatataca cctgttgatt
      781 ctaccatgct gaccatggaa atgatgatca ttagtaaaat cgaccatacg tcgaaagttg
      841 gtttaccaaa ataaaagggt tgtggaacat ggaacaacgg cgcatctcca atcgcgctca
      901 gatcaatcat tcccatgaaa gcagctaaga tgcttcctcc gactaaacca atcaataccg
      961 cgattgaacg aatgaaccct tttgccagca tttgcacgcc tacaattagg agaatcgtca
     1021 caaatgcaag tagcagattt gttttgtcac caaaatcagc tgctgttgca ttacctccac
     1081 ccattttctc gattgctaca ggaatcaaag tcaaaccaat aacggtgata accgttcctg
     1141 ttactatagg agggaatagt tttttaattt tagaaaagac accagaaact aacacaacaa
     1201 agatcccaga agcgataata gatccgtaga tagctcctac tccttcattg gttccgatca
     1261 agatcagtgg agctactgct tggatcgcac atcctaaaac gacaggcaag ccaattccga
     1321 aaaatcggtt aactgtcaat tgcaataatg tcgctaatcc acacataaag atatcaattg
     1381 aaatcaaata agtcatctgt tcttgattaa agcctagtcc tgtaccgatc aaaagaggca
     1441 ccgctactgc tcctgcatac atcgctagca gatgctgcag ccctaaaacc gcagcttttc
     1501 cattttgtgt ttctttttcc acgattatgc gtcctcctca agaaactcaa cttgtccatt
     1561 cgtcaaggaa gcaatacgcg caagagaaac gactctcaat cccatttctt caagtaactg
     1621 acgtccatct tggaaagact tttcgatgac gatcccgatt ccttctactt gcgctccagc
     1681 ttgctggcat aattcgatca atccttttgc cgcttgtccg tttgccaaga aatcgtcgat
     1741 aatcaatact ttgtcttctg aagataaaaa tttacgagaa atagaaatcg tacttgtcac
     1801 ttgtttcgta aaagaataaa cagaagctgt caataattct tcatccatcg tcaaactttt
     1861 tgcttttcta gcgaagatca ttggaacatc taattctctt gcagcaaata aagctggtgc
     1921 gatcccagaa gcttcaatgg tcacaacttt cgtgattcct tgttctttaa atacttctgc
     1981 aaaccgtttt cccatttgtt ccatcaatac agggtctact tggtgagtga cgaagctgtc
     2041 gacttttaat acgccctcgc ctaatatacg tccgtctttt tgaatacgtt caactaattc
     2101 tttcacttta tttgctcctt tatcatttat taataccgaa ataaccgctt ttgtgcaaaa
     2161 acctactatg tttctgttaa agaaaaacac cctccacttt atcataagaa aagaagggtg
     2221 aaatagccaa tcttttactt atagtccagc atttacggta ctggctagaa actgtcatcc
     2281 atattatgac gatatataag taggtatgta aaactaataa tagtgtaaat tcattttctt
     2341 gcaactgttt tgcacgtgaa ggcagtttgc catatttgaa taaaaaatgt tgtttcaaat
     2401 attgaaaaca gtctaacact actttctgaa actgacaacg tggtttactt atttttttgt
     2461 aaaatctaat gatttttaat gcattattcc tttttgttcg ggtttaaata gttattttta
     2521 ttttctccta tctaaaaaag cagcccacat aaaaagaagg catgacaaaa agcatgtcat
     2581 agcccctttt tccgaataaa tagtgttcaa aagacagcct atcggcaata aggcaagatt
     2641 ttataaacta cgggaagtaa tttccgtaaa tctattctta ttcttttaag ctgacaagct
     2701 cgtcaatcac tactgctttt cctgaagcaa tagaatgatt tgctgcgatt cctgtcaaaa
     2761 tcgacaaaat accatctacg tgagttgctg cccgattcca cggatcttct tctttttctt
     2821 caccaaatag atcatctaat aaacgcggat ctcctccgcc atgtccgccc gcttcttgtt
     2881 cgacgattac ctcatatggc tcaccgaaca tcggatagac atggatggac tgaagaacgg
     2941 ctgctccttc atccttttta tctccacctg cattgatgta agacttttcg atcactttca
     3001 tttcgatccg tcctttgctg ccattgaaag aaacattgaa tccttcccac ggcatatacg
     3061 cgtttagcga atacgtcaaa atcgcaccag tttcatattt gaccgttacg cctagtgtat
     3121 cttcaataga gatatcatcg gaaaatacac tgcggtcacg aatatatcca ctgtcttttt
     3181 ctgcttctaa atacatttct cgcaattcct cattggctga caggtctata gcaaatggat
     3241 cagctttagc tagttcgctt ccatagcatc gactgtaaaa tatttctact ccgcgtttct
     3301 ccgcattatg catcccataa aagtttaatc caccgaatgc aaataccttt tggggacggc
     3361 tgttcaacca aaaattcact agatcaaaat gatgggtaga tttatgaaca agaagcccgc
     3421 cgctattgtg cttatttcga tgccatcttc tgaaataatc tgccccgtgt tgcgtattta
     3481 acagccattc gaagtggata gaaaacacat caccaatcac atcactttct atcagttcct
     3541 ttatcttcgt gtgatacggt gcataacgat aattaaaagt gactcgcagg cttcttcccg
     3601 tctgtttttg gaccgttatt atttcttgag ctttttcctc atcgatagtc agaggttttt
     3661 cgcaaataac atcatatcct tttttcattg cacgaatgat ataatcatga tgcgttcgat
     3721 ctactgacgt aataatgact tcagccggcc gtacgtcatc cagtagttgc tcaaattgtg
     3781 atggcataca tgtctttact tttttctgtc catacttgtt cacaagtatc cgatttgcat
     3841 aatccatcct tgtttgagat acatcacaaa aaccgactag ttcacaagtt tgatgatacg
     3901 tagaggcaat tgcctcataa aaaaaacgcg ctctcccacc aagtccgact tgtacatatt
     3961 ttttcttcat ttaaagtcat tctccttttc ttttaagcaa aatactgtga caaatggcag
     4021 ccctggatac agtaatgcag tgagtaaaaa atcatgctgt atacaccaga acagtccata
     4081 caaaaggcat aaataccaca gaaacccctt gacgtttttt gacaacagcc agatattcct
     4141 tttgatcaaa taaacaagac tatctgcttt atcttctttg cagatatcaa ttagcgtact
     4201 aaaaaaacta tgggatacaa aaagcaacag acacaataga aaaggaatca gataccaagc
     4261 tccaatcttt cccactaata aataccaatc aactgcaaaa ataatgacaa acagctgaaa
     4321 caacgtaatg atgaaacaca tccgtttgat ttttcctttc ctccaacata cctttttttc
     4381 tgccaagtaa tggacagata gataataaga aggatacatc atcaaaaaat aaatcagata
     4441 gacaacacga gcagacagca ctaatcctaa agtgaacaaa catcctagaa ctaaagcgaa
     4501 aagagcatta ctgataagac tggttaaaaa aatccgacag acatagtagt tgagcttgta
     4561 aagaaacatc tcttctattg gctttttcat attcagccct tcagaccgct tgtactgatc
     4621 ccctggacaa gatatttatt gaagaataag aaaatcagaa aaaccggtag cagcgacaac
     4681 gtagacatcg caaacatact cgcgtaagtc gtactagatc cagcgtctgc aaataatttg
     4741 atggcaatcg ataccgtgta agatctcggc ttgtttaaat aaatcaatgg tcccatgaag
     4801 tcgtcccatt tccaataaaa ctggatgatg atcgtcgtga tgatcgaagg tttcagcaaa
     4861 ggaaaaataa ttttggtaaa gacactatac ttgctgcatc cgtcaataat cgctgattca
     4921 tctaactctt tggggatgct gaccatgaat tgcatgatct ggtaaatgaa aaatgcttgt
     4981 ccaaaaaaat gtggtaaaat cagcggcaca tacgtcccaa ccaaattcag ccgattgtaa
     5041 ataataaact gcggaatcaa aatcacttgt gcaggaatca tcatcgttac taacatgacg
     5101 acgaaccaga atctttttcc tttgaaatcg attctcgata atgcgtaagc aatacaagtc
     5161 gagctcagaa ctgtccctat cgtcgaaact gcagagatga caaacgaatt cctaaagaaa
     5221 gtaaaaaatg aaattccagc aaaccccgcc catccttttg cgtaattttc ccattgaaaa
     5281 gtctctggaa tcaaattcag cgaaccactc ataatctctt gatccgtttt aaaagaacct
     5341 gcgatcatcc ataagaccgg ataaatcatg actagaccta atagcaaaat aaaaccgtat
     5401 tgaaaccatt tcgttactgt attattcatt tcttcctcct ctcattcaca caacgaccca
     5461 tttttttgat gatttgaaaa tcagaaaggt gacagctccc ataatcaaca gcatgatcca
     5521 cgaaatagca ctagcatacc ccatagaatt aaaagaaaaa gcttggttat acacataaag
     5581 agcataaaag ttcgtggcat cattcggacc accttgggta atgataaatg cctgcgtaaa
     5641 agtcataaac gccgcgattg tctgcataac caagttatac aaaatgatcg gcgaaagaag
     5701 aggtaacgtg atcttgtaaa acgtctgcca tttgttcgct ccatcgatct ctgccgcttc
     5761 ataatacgag cctgggatct ctttgatacc tgcagcaaag atgatcattg ccgaaccgaa
     5821 ttgccaagca gacatcaaaa caagagggat catgaccagt ttttgatcgc taaaccagga
     5881 aagcgcaggt gcgcctaatc gttccaggaa aatattcact aacccgtttt tagaaaacaa
     5941 ctcccgccaa actagagcga tagaaatact gccaccgatc aaactaggca cataatacaa
     6001 agaacgaaag aaagtcgttc ctttgatatt cctcgttaaa agataagcaa tcaatagacc
     6061 aaagatcagt ttcacaggta cagatgcaaa tacatagaga aaagtgactc ggatggagtt
     6121 caaaaaacgg gcatcactca gcatctttat ataattatcc aagccgatcc atgagactgg
     6181 atttcccaaa cgataatcgg tgaacgaata ataaagtgac aagatcatcg gtatcactgt
     6241 tatcaataga aatcctagga tgaatggaga agcaaatacg taaccgacca cattgtcttg
     6301 atccaagaac cgttttagtt tttctttttt catgccttcc tcctgctgct atcctagaat
     6361 gtttgacgca aactcataaa gactctctgc tgcttcttct gctgttactt cttcataaat
     6421 gactttttca actaaaagat tgtattggtc ttgaatttcc ggattcaaag cactatcaaa
     6481 gacattcact tcgccgttgt ccatatcgga aatcatttct aaataatcaa atgtcgcttg
     6541 caaagttgca tcatctgcac gagcgagctg ttctcgaata ttcgacatga ttggtacacc
     6601 acgttctcct tttaaaattt cattggcttc ttcactgttt tgaaagaaat caataaactt
     6661 agcagcttct tctttatggg ctgagctttt tgccatactc agcatttgcg aagaacgtaa
     6721 agggatacca gattctccat ctgcttttct gcgaggtgga ttaatcaact gaatctccat
     6781 atcttcagga gcccctgaca atatttctgt caactggtta cttgatagaa acaccattgc
     6841 cgcttctcta tccctaaccg cctgaaaaga cagatccgtc gtacttgcag ctccaccacc
     6901 tggttctgga tgggcccctt tgtctaccag atttttacgc agctggaaaa aatctgttag
     6961 atagtctgga ttttcaaatc ccaagctgtt agatagatct tcccgattat aaaaattcaa
     7021 tcctttctca acttgtggta tttgaataat acagccagct ataaaatcct cgaatcttga
     7081 aatagcatag attcctaatt cttcagtgat ttgatagctg atttgttcaa attcatccca
     7141 tgtccaattt gaagctggtt cagggatatt taattgttca aatatatttt tattgtaagc
     7201 aatcgcaaac gaattcaccc cgttagacag tccaactaat tgcccgtcgt aagtcgtcgt
     7261 tgctaaatag gattcatctg cattagaaat atcaattatc ccttgctcca catattcatc
     7321 taatggttca atctgattga tgtattgagg aaattgattg ttcagttgga aaatgtccca
     7381 gatatcatct gatgctgcta gtgtacctaa ttttgtgtaa tatccttcat aatcataaaa
     7441 ttcataatcg atcgtcacat ggggattttt ttcttcataa agctcgatta cttttactgt
     7501 tgcatcgtgt cggacttgcg aaccccacca agccatgttc agcgtgattt ttttgtcatc
     7561 ttcttgccca caagccgtca agaagaataa cataacactt aaagctaaga tgattctcat
     7621 tcttttcttc attctctgca cctctacttc cattgttatt tttcaatata ctgtattttt
     7681 atacagaaca tatttattaa acgatctttt tcaaggggaa taaaaaccaa gacttgcctt
     7741 atcgttctgc ctaggatgaa caactcaata ctttcaagaa aaattagata gtgctgattc
     7801 ttctttcatc gctaaaaaca cttcttgctt caatgatggg aggataatta cgcaaagaaa
     7861 aaaggatatc agacaactgc ccaatatcct tgacatagct aaataagcat gcttcttcgt
     7921 gtgcgctctt ccctaaaaaa cagaaaaata actagtaatt ctgtatgaaa ttaggtacaa
     7981 tatacacgtc gatgcacaag cattgactat ggcaacttga ttggtcagtt cccgctgact
     8041 tttcaagccc cgacaattta gctgctacta aattgtcggg tgcctttttt agtttttatt
     8101 cgtttttcat tctatagaaa gcgcttcaat aaatcaagac atttttttgt tagaatgcta
     8161 ataataacaa aaggttttaa ctcttcttat gtacattaca ggtaaaaaaa caaaaaaatt
     8221 tctctttttt ttgaattctg aacaaatagt actggagtac aaaagacgag gaagagccac
     8281 tatctgctct tcctcgtctt ttgattctca cctgttattc atgattcttt ttctcagttt
     8341 cccgatattt ttccatttta cgactaaaaa gctctccgct acttggtggc gtatatgtga
     8401 tggcatttgc tcctgcttga atggtctcag caatactttc ttccgttgga ccacctgtcg
     8461 caatgatagg aagttcagga tattttttac gaaaatggcg tactgcttct gcagtttctt
     8521 tacctgcact gatattgata atcgaaatac cagctgctaa cttttcatct atttctgtat
     8581 atttcgacgt taccgtactg ataactggaa tatcaactac tcgacaaact tcttttactg
     8641 tctctaaagg cgtaggaccg ttcaaaacga cccctaacga accttgcgct tctgcgaaca
     8701 tgctcatgta ggaagaacga gcaccttgtg tcaaaccacc acctacaccg gagaaaactg
     8761 gaatgtcagc tgcctcaata atgcttttag taatcgctgg atgaggtgta aaaggataca
     8821 cagcaattac tgcattcgca tttgtattac gtatgatcgc aatatccgta gtaaagatga
     8881 tcgaacggat caattttcca aatatcttga tcccgcttgc ttctaaaatg atttccggta
     8941 ctcttacgat atcttttctc aattccgtca tgatttctgg cgtaaattgt tctgccatca
     9001 gccacttctc ctttgctatc tccggtcata ctgattcaat tgatacgtct cgatgacctg
     9061 gatgattttt tgcgcataac cggggtctgt cgcataacct gcatcttgca atgcttgtgc
     9121 ggcttcctga tagttggtgg ccgtaatcac tttttcatac agcgcaggat tccaatctac
     9181 tccttggacg aacagttgtg tatggtcatc catggactgt tcccatgaat catacacttt
     9241 gaagtctcct tggatagtta tccattcttc gttaacaaat tcctttgttt ccaggcttac
     9301 tttcttctga tccccataag ccttgatccc gaaaagatta ttgtatttgc tagccaaggt
     9361 actttttccc caatttgatt ctaatatagc ttgtcctaaa atgatactag gaagcactcc
     9421 ataaccatct tgtagttctt gggcatgcgg aacaagccgt tcaataaatt gctgatggga
     9481 ttgcgcttca ttttcttgct gattatcagt aaaaggagaa gacagattct tcaaggaaaa
     9541 aacaaaggct aagagtatca ctgctacacc ggcaaccact gctggccaac ggatattctt
     9601 gcgcttttgc tttttccact tttttttcgc catgatcaat tcctactttt catttaattc
     9661 attcagatat tcttctagcg tcaattggtg tttggtgata tactcagcgt tttctttacc
     9721 gacataacgt aagtgccaag gttcatatgt gatcttagtt atatcttcgt gtccttttgg
     9781 ataacgaacg ataaatccat actgcgcagc attatctgcg atccatttag ctccaggctg
     9841 atctccgtaa cttgcatcca atacagtaga aggataactg ttgtaccagt tttgatcgac
     9901 gacatcgact gccagacctg tgtggtgttc gctgtatcct ggctccgtaa tcgtttcttt
     9961 agtaatcttt gtcgcctcct cttctgatac accttgacga ctcatcacat cttggacatt
    10021 ttgttcgaaa acttgttgtt gggatgatac agaccggtaa gctgagacca tcactaatgg
    10081 aaatccagct gcttcagcag ccttggcaaa tgcttcatat tctggagcaa tgcgtttgtc
    10141 gatcaagtat ccgttactta atgatgccaa ttgagtagac tcatcaattt ctttttctaa
    10201 tttgtgatct ggaccaacca aaactagctt ccaatcgtct gttttaacat ctggcaattg
    10261 tttggacgta cgatcttttg tttctttgtc ctttaccttg ctgctggttg tttgactgtt
    10321 cttttcttta ctagcagttg ttttggtgct tgttcgttcg actgcttgat tgccggcttg
    10381 aacatgcgaa tcatcatcgc taaaaataac atagcccatt gctgcgatca taatcacaac
    10441 tgctccaaat cctaaaaatt tattcacttg aaaaatcctt tcgttgttct tcacgttccc
    10501 cattcgcctc atgaacgtaa gtttcttcca gattttcatt cacccaatcg agaagttcga
    10561 ttttttctcc ttcgatctgt ttttgaacat ctgctggcag acgaaaattc aaaatatcat
    10621 catagcccca ttcatcatat tgaatactta ctttcaagtc aaggtatcta ccagcctgct
    10681 gattattttt caaagggatc agctcgactt ttgctgcttt gctgctaagc catttttgcg
    10741 cgatgcgtgt tttgatttgc ttgtactctg caacggcaac gtttcgtttt tcgggaaaaa
    10801 taatcgtttg acgcaaaatc ttttgcgtta gcatccattc ataatcagtg aaaagatttt
    10861 ttactttctg catcgcttca gatggcagat ttttctcgcc atctttccat ttttgccatt
    10921 gttctggact gatcagcaag tattcatcta aaaaattttt ttcagtttca aactgttcta
    10981 ataatacact cataataaga tcaatatata cttgatgcac gtttatcccc tccttttaac
    11041 tattctactt aagttttgat caaaaagcac ctcttaaatc gctgctttca tttttttaat
    11101 gtttcaagtc ctctcctctt tattcttcgt gatttttgtt ataataatag acagacaaga
    11161 aactcaatcc aactatgaag gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg
    11221 gtcttggtac catcgcacat gagtttgccg aaagcttcaa tcaagagacg agcgaactaa
    11281 cagcagttgc ttccagaact tcagaaaaag cggaaaactt cgctcaccgt tacaatattc
    11341 caaaagctta cggaagctat caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca
    11401 ttgctgtacc aaatagacag catatcgacc atatcttggc agcattggaa gcaggcaaac
    11461 acgttctatg tgaaaaagcc atcacgatga ataaaaaaga actggctgac gctatgcgct
    11521 tagcagaaga aaagaatttg atcttagcag aagcgatgac aatcttcaac atgccacttt
    11581 atcagcagtt acgctcaata atggatacag gaaaactggg ggctttaaaa atgatccaag
    11641 cacctttcgg cagttacaaa gaaccagacc ctaaaaaccg gttcttcaat ccagaattag
    11701 ctggaggagc tctgttggat atcggtacgt atgctgtatc tttcgctcgt ttctttttga
    11761 gctctcaacc tgaagtagtt gcctctacaa tggttccttt tgaaacaggc gtggacgaac
    11821 aatccgtcac tatcttgcgt aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc
    11881 aggcaaaaat gccaaaagtc ggtatcgttg cctttgagaa cggttatgtg acgattgctg
    11941 attacccgcg tgctgatcgt gcggagatct tatttacaga tggtacaaag gaattcatcg
    12001 aaagcgggaa tacatctgaa gcaatgaatt acgaaataaa gaatatggta cacatgattg
    12061 aaggtgactt gcccaatcgc tctctcttct tgacaaaaga tgtcatcgaa atcttggatc
    12121 aaatgcaagt tctatggaaa aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca
    12181 tcaagtaata agaacagcca aacaaaaata gctcctccta tttttcgttt ggctggactt
    12241 attaccgcag aggaaagctt ttgaacaccg tttaagaaag aggttgtgag cctaccgttc
    12301 agctccgagt cacaaggagc aatttcaaaa aacagcttct catgtttgtt gaatattgtg
    12361 acttgtgacc gaggagttga caggagaaca ccgtttaaag aagaggttgt gaaatcagct
    12421 ttttgacctg aatattagct gaattcacaa cctctatttt tggatttttt gttgggagaa
    12481 ttctgccttg atttcaataa aacttttaaa agactgcaaa aactggaaca attctgctcg
    12541 atcctcgaat tcagggcgac tagtcggcag aggcatttgc cgatacaata cataaacttc
    12601 ttcaatacgt gttaaaattt gtctgccatc atttgactct gaaaatgttt ctgcagtata
    12661 aatcaataaa ccatagatat gttttccata ataagctggt tgctggattc tctctagatt
    12721 ttccagcatg tctttaatca cattggcctg tgctcttctc atagagaagt aagtttcgta
    12781 gtatagcggc tgtctgagcc agtagttttc ttgatgttct ctggcatctt cttggctttc
    12841 gcgaatgaat gtcagtaaat gttcgcattt gatctgcaca tcttctttat tttctgataa
    12901 aagaaattct gccatttgtc tcaaaatatt tcgaaattct ttttcgatct gtatttggtt
    12961 ttccttcagc tgcttcgtac gatctggcat gtaaacattt gcaagcaaag caaatactaa
    13021 tccaatcgcc attaatcccg cttcattgcc gatcagctgc caagacatgt tttcctccac
    13081 taaataatga gtgaccaaaa ccgaatttac tactatccct tcggttaatt tgaagcgaga
    13141 ggcaagcggg atgaacagga ataaaaaaag actgaagcca aaaatagtat acccaacagc
    13201 aaaaaaactg aacagtgctg ttgccgtagc taatataaag gctgcgattc gattcatgcc
    13261 cgtacgcaat gtagagcgct tggtatttcc tacacttaat aatgcaatga tccctgcagc
    13321 tggccaatac aacagcgata agctacccgc tgtgaccatc gaaaagaaga cactgatcac
    13381 tgttttgact gttcgcattc ctatacgcat ttacttcccc cttttttatt ttaattatac
    13441 ttgaataatg aaaaactagc tatcagacaa aagtttaggt atgatagtaa aaagaccaaa
    13501 aaggagaatc tctatgcaat ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc
    13561 cttagctgtt tttttatgta tcttgttttt tcatttgact caacgaggaa tcccgatgat
    13621 tgctgcatta tctgctgttt tttctttgcg tcaggacttg actacaaccg tttcttttgg
    13681 aaagtctagg atcatcggca atacattagg tggtacatta gctatcattt atttttttgt
    13741 caaaagctat tttcacaatg attttttggt agaattgatc ttgcttccta ttctagttat
    13801 tattgtgatc gtcgtgtcag atggtattaa caataattcc ggtatcattt ccgcaatagc
    13861 tacgttgttg ctgattgcgt tgagcatccc tcaaggcgaa tccgctttgt atgctatcca
    13921 acgtgtacta gatactttta tagggacatt cattgcaatc ggtatcaatt tctttcttcg
    13981 tcctcctgaa acagaaaaga aggaagaact atcagaagat ttggtcgaac tccaaaagaa
    14041 agaacgattg ctaagaacaa atttacaaga gatagaagct aagatccaaa aacaaaagaa
    14101 aaatagcgag gactaatttt cttttgcaat aaaataaaaa aatggagaga agaactttaa
    14161 aaagtcttcc tccatttttg attgaacggt gaagtgtact actttattct tccttcatcg
    14221 agttttgttc tgcctctttt ggcgcttcat acatatttac ttggctagta gtcccacgtg
    14281 tcgacaaatt ctctacgatt tccttcaagt caagtcctgt tgtcgctttt agtgtttctt
    14341 gagtagaaga aagcaaattt gtggcataat ttgtcactcg gttcgctcca ctggtttctc
    14401 ctgaactgct tcctgtatcg acaacagaga ttttttcgat atttcctagc ggctgtgctg
    14461 cttcgcgcat caattgcggt agcatatcga taaccatact caagacagct gcttcgccat
    14521 attccttgaa ggcattggcg atcttttgtt ttgcttcggc ttctgctttt ccttttgcta
    14581 gtgctgcttc ggcttctgct tgtccagcta aacgtgtctt attggcttcc gcttctgcca
    14641 gtgcttccac acggaatcgt tctgcttcgg cttcagtcac ttctttgact ttttgagctt
    14701 gtgcttcttg ttctttagcg taccgatcgg catctgcttt tttcttcact tccgaatcat
    14761 attgcttttc acgacgtgtg atttcttttt cttccagttc gatttgtttt tggcgttcta
    14821 cgattttaat ctgcatttcc tgttcgatta cttgttgttg tgctcgggca ctttctaaat
    14881 tgtaggcttg gtctgctttt gctttagcaa catcttgttc ttgtttgtag gttgctaatt
    14941 tcagttcttt ttcttttaat gattctgcga tctctgtttg tcgctgtaat tcagctgctt
    15001 gtgattcttt ttctgcttga gcttttttga tacgcgtttc ttttagagct tccgcttctg
    15061 ctatatctgc atctcttttg acttgggcga ttcttggttt ccctaaagag tcaagataac
    15121 cgtttttgtc tctgacctct ttgatagtga aagaaacgat gatcaacccc atttttgcaa
    15181 ggtctacaga agcgacttct tgtacgcttt gactgaactt atcgcgattt tgatagattt
    15241 cttctacagt catagaacca agaatcgagc gcaagtgtcc ttctaatact tctcttgctt
    15301 cattttccag ttcttctctt gtcttgccta aaaactgttc tgctgctgta gcaatctctt
    15361 ctacagatga accgattttg atgatcgaag taccatcaca catcaccggt acgccttgtt
    15421 ccgtatacac ttcaggggtg gagacgtcca atttactcga taatagactg atccgattcg
    15481 aacgttgaaa aactggcaag acgaatgcgc cacctccacg cacaattttg attttgttat
    15541 tgctttcatc aatgtgcaca tttttgcttc ctaaataact tccactgatg atcaacgctt
    15601 catccggttt tgctgtctgg tacttcgcta caaagacgat cagcaacatc agtaaaatca
    15661 aagctgcgat tgcgatcgcg ataaaaacaa ctggtatttc catttaacac acgctccttt
    15721 aagcttttat catgctttca taaggcatga cataacagat tctttccttg atctcaatga
    15781 tcaatacttt cgttccccgc tcagctgtct gttcttgggg gctgtaaaaa caagctggac
    15841 gactaatgga accggtaaca cttttcaact gaatttctcc catacctcgt acagggatcg
    15901 gtgtgatcac aacagctact tgtccttcca gagttttttc agaagacgac atcgttgatt
    15961 cggcatgttt catcggcatc aatatataga aattaagcaa aaataccaac actatcgata
    16021 tgcctccact agcaataaat aaaaataaag atgattgatc tgttaaacgc tcacctagaa
    16081 aaccaaataa gcttgtgaaa gttaaccaag ggataataag catcggatca agcgggccat
    16141 caaaatcgaa tacatctcca aaaataatca ataggactgc caatcctgca caaatcagca
    16201 atgtatacca ataaatcgtc tcaagtgcga ttccccctat catatacacg cctccttcca
    16261 tttctacgtc tatcatacca atgtttgaaa attctgtata gcttctttta ccgactcagt
    16321 ggtgtttttt ataggaaggt tgtgtttatc gttgacaatt tgttaaattt ttgttttcat
    16381 gttaaaattt tcacaataaa ttctttcgag gtagcctatg tttccttttt ttaagaaaaa
    16441 accattaaag ctccagaaca cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt
    16501 gattgtttct gccatattga tcaggaattt tattctttcg atgaaccgat ctagtgtgtc
    16561 agataaaatc tctagcattg ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct
    16621 gcagactggt gacccgacac atgcgattca aggaataaca aatgaaatca tgattgcatc
    16681 gaatttagat tttatcgttg tcatggaccc tgaaggcatt cgatattctc acgcagaccc
    16741 aagttacatc ggtcttcctt tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca
    16801 agaatttttc tctgaaaaag aaggaatgtt agggttcggc ttccgctatt tcgtccctat
    16861 ttattatgat caagagatta ttggtgcagt ttgtgttggc ttaacaaaca acacgattga
    16921 caatcaagtc aaagatggac aaaaaatgat tgttttttca ttgattattg gacttagcgt
    16981 aggcatcata ggcgccgttt ttcttgccca aaaaatgaaa aaagtactgc ttggacttga
    17041 accacaagaa atagcagaac agttaagtga aaaaacgatt atcgaaaatg aagtagatga
    17101 aggaatctta gccatctctt ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa
    17161 cattttctac caaacagagc agctattgga tttcgaagta ggaaaaaaat cagtgataat
    17221 ctatatgacg ttttgtttaa agagacgatt gagcatcaaa cgaccgttcg gactcaatcc
    17281 atgtatttaa atgggataga aactatttct agcgttacac ctctttttag caatgaccgt
    17341 tttttaggag cagtcgcaac tttacgaaat cgatcagaag taatgaaatt aatggaggag
    17401 cttgggggga cgaaacaata tataaactcc atgagagctc aaacccatga attcatgaat
    17461 aaacttcatg tcatttctgg tttgattgat ttgaagaagt atgaagaagt cacccatttt
    17521 atccagcagc taaatcaaaa ttataaaaaa gagattggcc aagtctcaag tctgatcaaa
    17581 gtacctgcaa ttgccggttt tttattaggc aaaataaacg aagccaaaga acaatctgtt
    17641 gaaatgctgt tagaaagcga ttcttttatc cctaaaatgg agatggatga ctccatccat
    17701 tcacttttgc aagtgttagg gaacttactt gataatgcaa aagaagccgt gctacaaaaa
    17761 catgaaagaa acggacaggt ccggattcat ttaaactatg acacagaagc tgaagtattc
    17821 atcgtgaccg tctgcgatga cggtgtcggg atatcagaag aaatctcaga aaaaatattt
    17881 acgctaggtt tttcgacgaa gggaatcaat cgtgggtttg gattgaatct catccattca
    17941 atcgtcaaac gccacggtgg atttttagaa ttttccaaac aaacaaatgg cggtacaatc
    18001 gtctatctag aattaccaaa aaaggaagat caaaatgatg acaaatgtcc tgataatcga
    18061 agatgatcca atggttgcag aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa
    18121 cgtaatcgaa agtttcagaa atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc
    18181 agatctgctg ctgcttgatg tatacatgcc tggcgaaagt ggtatccatt ttttatcttg
    18241 gcttagacag caaggaaatg atatcccagt gattttgatc actgctgcag atgatatcga
    18301 aaccataaag aatgtgatcc gctacggtgc cttagattat ttgatcaaac cgttcacttt
    18361 gcaacgtttt cagctggcga tcgaaaagta tcttgctttc tctcagacaa tccaaacgac
    18421 tgaaaaagcc gatcagaaat tgatcgatca agtattcaac cacaccactc ccattcacga
    18481 aataccatca ttcatcgatt tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag
    18541 tgcatttatt ctaaaagaag atactatttt ttcaacaaaa gaactggctc aaaaaagctc
    18601 actgtcacgt atatccgtca agaaatattt gaatttttta gtagaacaag atatattgaa
    18661 cgaaagaatc aaatatttgg aaatcggccg gccgctaacc tgttatgaat tgacaaagaa
    18721 tgggcggaaa aagctcacag attactgttg agtcatcgaa ttatcccata caaaatagtc
    18781 caagtataca gaagtaacaa ctgtttactt ggactatttt taaacatttt attgacgaat
    18841 atccatgttt tcttatctta attgacgggc tttaggaaga gatacctcat ttttcgtttt
    18901 tccagtttct attatttcac gaaagttctc gaaacttgtc gaaatcatat ttgataatgc
    18961 ctcatctgta tttgaaccta catgcggggt tacaagaact ctagggtaca tttctactaa
    19021 ttgctggata gcagggtctg gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc
    19081 tgtttcattt gcaaaaacat cggttgcaaa accagctaaa tgattgcttt tcaaagctct
    19141 taaaatagct tcattatctt gaagctctcc acgggctgta ttaatcaaga tgctaccttt
    19201 tttcatttga gctaaaaatc cttcattgat catttgatcg ttttcacctg ggatatgagg
    19261 tacatgaata ctgacaatat cgcttttggc taataactct tcaagcgagt ggaacgtcaa
    19321 tactttttta gcagcttctg tctgatgaat atcgtagccg atcactttgg ctcctagtcc
    19381 cttaaacaaa acagcttccg tcaaccctat cctgcctgtt cctatgatcc ctactgtgca
    19441 atttctgatt tcacgactaa acatttgctc atccactcga aaatctttga aagatgttcg
    19501 gatcgtcgta tacgcagtat ttctcaaaag catcatggca aaagtcaaag ataactctgc
    19561 tatcgcattt ggcgaatacg atggtacacg ggctactgtc ataccaaaat cagctgccgc
    19621 ttgtagatcg atatgattga ctcccacagt ccgtgtaaaa acataacgaa tcccatattc
    19681 ctgcattttt gcaagattgg tctcatctgc tatacaattt cccctcaaca atacagcatc
    19741 atggtccttt gctgacacga tattgtcatg tgtcaacagt tcttcataaa gactgagatc
    19801 ataatggtaa atattcaatt tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca
    19861 tgcgattctc atactttctt cctccatttc ttatgaaaat aaacctatgc catgaatcat
    19921 ttgtaataca acgatccata caggtgtcag taccacagca aaaatagttg acagtaagga
    19981 tgcattggag gctaaaagtg cctccctatc aaaactgata gcataggctg ctgccactgt
    20041 tgcagctggt gtagccatca tgataacgat tgttgccagt gcttcaaaat tgactggtag
    20101 aatattcgtt gccgtcaaaa ctgctagaat cacgatattc aatgcaggta caaataccac
    20161 tttgactaga ctgtaatacc atgaagtttt ttcagatgca gcttttttga agcttacttc
    20221 acctaatgtt gcgccgattg ctagccacgc caatggtgaa cttaaaccag ctaaataatc
    20281 catcggctga aatagccacg gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat
    20341 ctctccttct gcacctgtaa ctgacacttg tggcagatac ccttggaaca gccaaatgaa
    20401 aaggccggca aaagtagcga taatgattgg attgacgaac atcgctttga tatttttagc
    20461 ttccatcttc aagccactca tttttatgta tccataagaa tacaaaaaga ttcggtagcc
    20521 aatattgaaa attgaggaat acatcacgcc gacaggacca taaatcgcac tgacgatagg
    20581 cgttccgaaa aaggtcgtag aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc
    20641 ccctttatag cttaagaaaa aaggttttga aactagaata agaataatat aaataactaa
    20701 tccccaaatc aagacattga ttccttgcat cagtaattct gtattgatat cttgcatgaa
    20761 agctcgaaat gccagtgctg gcaaagccac tgttaagacg actttcgata gaatttttcc
    20821 aacagattca gaaaaaatcc ccttttttct acaaacaaac cctaataata taataaaaac
    20881 tgttgaagtg attgcactga caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa
    20941 taagttcatc tttctcctcc taattatgtt ca
//
LOCUS       DAGCWU010000032        20884 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.032, whole genome shotgun sequence.
ACCESSION   DAGCWU010000032 DAGCWU010000000
VERSION     DAGCWU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.032"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(16..1026)
                     /locus_tag="MBZ71_000948"
     CDS             complement(16..1026)
                     /locus_tag="MBZ71_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBT4428919.1"
                     /translation="MKKKIFVGALVALFLLPVNAFAYTINNEFNLGANEGSSQVSNNQ
                     YILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKETFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVNANGVSRPD
                     VNQSYSTYGNVGYHVSFNMRNFSNKKVYVMMRATNDPEGNTKDGAQDFHDKRWYLNIP
                     KR"
     gene            complement(1037..1234)
                     /locus_tag="MBZ71_000949"
     CDS             complement(1037..1234)
                     /locus_tag="MBZ71_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBT4428920.1"
                     /translation="MILPDKYYQIIKWAVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            complement(1248..1490)
                     /locus_tag="MBZ71_000950"
     CDS             complement(1248..1490)
                     /locus_tag="MBZ71_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBT4428921.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            complement(1525..1662)
                     /locus_tag="MBZ71_000951"
     CDS             complement(1525..1662)
                     /locus_tag="MBZ71_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBT4428922.1"
                     /translation="MFSFSDIKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            complement(1662..2069)
                     /locus_tag="MBZ71_000952"
     CDS             complement(1662..2069)
                     /locus_tag="MBZ71_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428923.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQNYSKKLELLENLSIGLQVDNTALKKSNEELTEK
                     ADSMAQLNAKLMFNDLTINKKIEAIEKQIGGAS"
     gene            complement(2097..2621)
                     /locus_tag="MBZ71_000953"
     CDS             complement(2097..2621)
                     /locus_tag="MBZ71_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428924.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPYGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            complement(2621..2989)
                     /locus_tag="MBZ71_000954"
     CDS             complement(2621..2989)
                     /locus_tag="MBZ71_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428925.1"
                     /translation="MTDIVKVKQNDVQVYPQTHWDAVEGKPETIKGDKGDPGQAATIT
                     VGTVTSGTIASVTNAGTASAAKFNFVLPKGDKGDKGDPGANATTTAVATTTANGLMSK
                     EDKVKLDGLANITFEKVGTV"
     gene            complement(2994..3782)
                     /locus_tag="MBZ71_000955"
     CDS             complement(2994..3782)
                     /locus_tag="MBZ71_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428926.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITSEMYQKILNSGEGDYVLPVATI
                     DRLGGIKIGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGKI
                     NAEVTGFYVLPTASEFVKGGIRVGEGLTMTDDVLSADKQFNYTAGANISISNTGVISA
                     TGGGEGGGVSQEYVDQKMSEAYQNAQAYTDSKIPNVSFEKVGEV"
     gene            complement(3782..4690)
                     /locus_tag="MBZ71_000956"
     CDS             complement(3782..4690)
                     /locus_tag="MBZ71_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBT4428927.1"
                     /translation="MAKWNVVLSTTEPYNYVGMIQVRQGNKNSEVMEATITENGMPYD
                     LTGCKVYFESVVGDKYPVQLGTKVIDAKKGKIQYTFDQYSMQCLHRQTADFIIYRDDE
                     LIATTQDFSYFVIKAVSKTEGEMGSYWQTVEDLIADMTAFINENKGDFTDWMNARKKE
                     FEQWRQDQQNTFEAWREGQETDYLKWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(4656..5174)
                     /locus_tag="MBZ71_000957"
     CDS             complement(4656..5174)
                     /locus_tag="MBZ71_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBT4428928.1"
                     /translation="MIEEKGLNHLKSLLNQPIGNHQCYALSAEYAGVMIGPDMGAGTQ
                     YEIKVRHGNVFSAAEIGRAYPWSLYLWTVIAHPEYDQLVVGSIINWERNAKISDTFES
                     HEYYGHTGVIKGLENGRIQTYEQNAESGGIVAEYDREFFGSGQIASICIPPDFEKGVV
                     INGKVERSTQHN"
     gene            complement(5185..8007)
                     /locus_tag="MBZ71_000958"
     CDS             complement(5185..8007)
                     /locus_tag="MBZ71_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4428929.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNIKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLIGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGEMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFDIAMTSKGEIVANFI
                     KVGIINAEVLESSINDLGDVLKIVKGTLQIWNDNKKIMELTKKGMEFWNTKESIGTIG
                     TTDSAGNPFPGASTPTPLEDNSLVIRTNGDGKYILISPKEGKGWVILGNGTSILFGSL
                     NLQEKLNAFGDAEFMKNVNIRGKLTINGQEVFPGQGGSGNNDGGSWNGMYPPEVTSQA
                     DKFAWELWVMLLSRGYSKASIAGILGNVQGEAGAAMNPDIAQVGGPAYGIVQWDGSAY
                     PLVPPATWDGRTYVQNLMRAAGITEDYRTMSAQGKLLDWTMYNGQWLGIVQPASVSGF
                     KAMTDPAAAAYTFERNYERPATTHPERQGWAVNWYNKFKDLQVPSAGSILSTAKSLMG
                     YFHYSQPLRWNFGSVENPDRNGYADCSSFVWLALTKAGYKTATRGTLWYTGSMAADAR
                     GARQYLTEISPNEAKAGDIIIVNLGAGAGNDGHTAILAEDWKGYSTSIVEMGGMNSNG
                     VGIGRVDWSFGYLLNGGDVCLARAKK"
     gene            complement(8004..8708)
                     /locus_tag="MBZ71_000959"
     CDS             complement(8004..8708)
                     /locus_tag="MBZ71_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4428930.1"
                     /translation="MNPDEPNFIWKNRNAVIDMDCIIEVELPDIMPNKRYETYTVQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLDAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAIGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(8720..11029)
                     /locus_tag="MBZ71_000960"
     CDS             complement(8720..11029)
                     /locus_tag="MBZ71_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBT4428931.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAASLAAFGEVDEALDTIITKTGATGKVADSLGESFKKVGANTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAVMARQAIEAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFNEGVTLMGQFEQAGVDSGAALSSL
                     SKATVAYAKDGKTLSQGMGELQDKIKNASSETDAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKIADESQGSVGETFEATLDPIDKSNQAMNNAKLALADVGESVQISLLPFFE
                     MAIDALKSFKGWWDSLDQGTKNWIITLAGIAAVIGPALVVIGTLMSSVTKITAGVKDL
                     ATVWSGLGKLFGLSGGWFAVAVIAIAALVAGLVWAYNNVEWFRNGVNAFFQGVSDIAV
                     EVFNFMGGYISNIFGGIIQNFQNFFDAGKRIFTGFIDFITGIFTGDWERAWNGVVNIF
                     GGIFDGIAAMAKAPFNGMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANQQAIYDLGGVPI"
     gene            complement(11224..11688)
                     /locus_tag="MBZ71_000961"
     CDS             complement(11224..11688)
                     /locus_tag="MBZ71_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428932.1"
                     /translation="MARLADYGIHVDDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            complement(11688..12314)
                     /locus_tag="MBZ71_000962"
     CDS             complement(11688..12314)
                     /locus_tag="MBZ71_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4428933.1"
                     /translation="MGKQDTLYFEGLDDILIAMMATPDTVGTAPTYSEVVRLPIGTKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANFVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWETLAKVGGGK"
     gene            complement(12321..12686)
                     /locus_tag="MBZ71_000963"
     CDS             complement(12321..12686)
                     /locus_tag="MBZ71_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBT4428934.1"
                     /translation="MPSNKNGFSEISDYLGNLSRVDPKKLSLESLEEAAKFYLEQLLP
                     NIPKSLLKKKHMSEQIKVVVDDDRVKVQFEETAFYWRFVENGATNQRAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            complement(12676..13014)
                     /locus_tag="MBZ71_000964"
     CDS             complement(12676..13014)
                     /locus_tag="MBZ71_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428935.1"
                     /translation="MSISFDELKTALKSTKLPVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQISFFTLGTEQDLAVIEEALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCMENAK"
     gene            complement(13004..13330)
                     /locus_tag="MBZ71_000965"
     CDS             complement(13004..13330)
                     /locus_tag="MBZ71_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBT4428936.1"
                     /translation="MPKRQTNNLRWKAELLEIKTGTDENDRPTTVYESKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDTREMEL
                     SLAYVD"
     gene            complement(13308..13589)
                     /locus_tag="MBZ71_000966"
     CDS             complement(13308..13589)
                     /locus_tag="MBZ71_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBT4428937.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKALSAITPFIVQGVIQNAETTDQ"
     gene            complement(13591..14955)
                     /locus_tag="MBZ71_000967"
     CDS             complement(13591..14955)
                     /locus_tag="MBZ71_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBT4428938.1"
                     /translation="MNKKLLKKLQARHEQRLTELRGRIESGEVREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGKPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(14967..15539)
                     /locus_tag="MBZ71_000968"
     CDS             complement(14967..15539)
                     /locus_tag="MBZ71_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBT4428939.1"
                     /translation="MKKKEIRTFDITNLSTRDVTENNSRIVTGYAAVFNSRTLLWEGL
                     EEVISPGAFSKALSNSDVRCLFDHDWGKVLGRTKSGTLRLEEDERGLKFEVELPNTTD
                     ANNLIESMSRGDIDQCSFGFIPTEETWDYNTDPVLRTLNEVELFEVSIVSLPAYQDTE
                     AALARSKQEVQQDIATRKKMIQKINGALNA"
     gene            complement(15511..16698)
                     /locus_tag="MBZ71_000969"
     CDS             complement(15511..16698)
                     /locus_tag="MBZ71_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBT4428940.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNDSDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQNVKGGDGNEKEGN
                     SDI"
     gene            complement(16762..18489)
                     /locus_tag="MBZ71_000970"
     CDS             complement(16762..18489)
                     /locus_tag="MBZ71_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBT4428941.1"
                     /translation="MIEPGVNYADLFAKEVRKKPGKYPKTVRLAVDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFILEPWEKFIYSWMYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAATILN
                     NPILLEGTKIFKGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGNLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(18486..18938)
                     /locus_tag="MBZ71_000971"
     CDS             complement(18486..18938)
                     /locus_tag="MBZ71_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBT4428942.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            complement(19050..19430)
                     /locus_tag="MBZ71_000972"
     CDS             complement(19050..19430)
                     /locus_tag="MBZ71_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBT4428943.1"
                     /translation="MKYCQFDGCTNKIAKGIYCAEHKRSSKSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(19427..19813)
                     /locus_tag="MBZ71_000973"
     CDS             complement(19427..19813)
                     /locus_tag="MBZ71_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428944.1"
                     /translation="MAMIKIRTTITGTEYWDSEKKKTVVVPKGQEPDFEATEEKGILS
                     DGKTFVAVNGELITNSSEILDSDGNTAADFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAVRAKGDIINLIREAE"
     gene            complement(19794..20099)
                     /locus_tag="MBZ71_000974"
     CDS             complement(19794..20099)
                     /locus_tag="MBZ71_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428945.1"
                     /translation="MNDFHEAMLMANVPTNMGDVYKKAIEAENSPTGLRNNWNGTYAN
                     VQVDKDNRGIAMDVLVISLLSHTLPNLQETVEWYERMGAKVISTNYKGENQNGNDQN"
     gene            20743..>20884
                     /locus_tag="MBZ71_000975"
     CDS             20743..>20884
                     /locus_tag="MBZ71_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428946.1"
                     /translation="MLTNEQRAHDLAIATLPFVRDIVKSQIIEGKDAKFDAYFEYIKL
                     YNQ"
BASE COUNT         5709 a         4658 c         3408 g         7109 t
ORIGIN      
        1 tcgaggggct attttttatc gtttcggaat attcaaatac caccgtttat cgtgaaaatc
       61 ttgcgctcca tctttagtgt ttccttctgg atcattagtt gctcgcatca tgacgtatac
      121 tttcttatta gagaagttac gcatgttaaa agatacatga taaccaacgt ttccataagt
      181 actataagat tggtttacat ctggacgtga aacgccatta gcatttactc gtgctaactc
      241 ttttccagta ttatagtcca tgataaaaat gtactcgtat ttatagttag caatgtgcca
      301 gccagccaca tgcaagtttg cgttttcgat ttctccaaac tgatcaatgt gggcgtaatt
      361 tgttccatca gtgagtgtag gatttgctgc acctgctcgt gttgggtcaa tgacaggctt
      421 gttctcagaa gttgttggat tttcatctgt aaatccatgg gctaaatcgt aagccagttt
      481 ttccttactc acacccattt ctgacaagta tccataagga tcagtgtgat cgccccaaat
      541 gttttgagtt acccataaat gtgatttgat tcctggttgg ttataaggcg tgtccaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacatatg ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gactgttagc
      721 attagcatac gaaccagcac cgtactgtac ataaccaggt tgtccaactt ggtaaacaat
      781 tccaccgtct cccacaatat aagcagtata agcactagtc catgaacgtt gcatatattg
      841 cgcttcattg cgtcctgtcg cagtttcgtt tgctgtttca tgcaatagaa tatattggtt
      901 atttgatact tgagaactac cttcatttgc acctaaatta aattcattgt tgatcgtata
      961 ggcaaatgcg tttaccggca acaaaaaaag agctacgaga gctcctacga agattttctt
     1021 tttcatttgt ttcctcctat tttttcaaat tataagccga cacaccagtg ataacgccta
     1081 aaaatgttgc tgctgcattg atagtcagaa ctgtcatatc tgttccattc catccatatg
     1141 cttttcctaa cgtggctact aaaacagatg cagctggtaa tactgttaaa actgcccatt
     1201 taatgatttg ataatactta tctggtaaaa tcatttcttc gcttccttta tagtttagtt
     1261 aggaaatagc caatgattgt aatgcctaag ccaatcatat agccccaagc ccatttatta
     1321 ttgttcttca tttccttgat gtcttctgca ttgtttagtg ctactgaata ggctttatct
     1381 gccaaatctt ttgcactatc agccttttct ctaagtgatt cgtagttgtc taattttgtt
     1441 tcaattctta ctaatctctc cacaacgtct tggagcgctt cttctttcat gttccaccaa
     1501 ctttccaaca aaaaaacgca tcacttaaga tgcgctctct tctttgctaa taattttatc
     1561 tgcttcttcg tctgtaatgc atagtggaac gaataatcga acttgatcgt cagtaaaaca
     1621 gccccaatca tacatcattt ttatatcgct aaaactaaac attatgaagc acctccaatt
     1681 tgtttttcta ttgcttcaat ttttttatta attgttaaat cattaaacat cagtttggcg
     1741 ttcaactgcg ccatcgaatc tgctttttcc gttaactctt catttgattt tttcaatgca
     1801 gtattatcta cctgtaaacc tatagataaa ttttctagta gttctaattt tttagaataa
     1861 ttttgtgtaa ctgcttcttc ccacttattt tcagtgaagt taaaaaattg cgactgttgt
     1921 cttcttccga aaccttcatc cgtttcatct tccttctttt caaacaaaat aggtggaact
     1981 tcgacaaatg gtaaagatgt tggaaagtta tcttctactt gatgttcttc atatcccatt
     2041 ggatacaata ctttataaat tgttttcatt ttactcttcc tccaattagt aatctgttaa
     2101 gccggccaag gatcttgagt gatccacata cctgaaatat atgaactacc tgaacctgat
     2161 ttggcttgta tgatgctact ttgatcaatg aatgcacgtg catccgctgg ctgacttgca
     2221 tttctaacta gtgaaagcgc ggtttgcgct ggatagcctt gatcacgttt gaaaccgtac
     2281 gggattttta aaatccaacg agtttctgat ccttcgggcc atgtgccaca tttaaaatta
     2341 aaagtaagaa atacgatgtt tccgattctg attatttttc cattcacatc agttacgttt
     2401 gttgtatcac ttcgatcagc tagtgtgata gctcgctcga tcatacctgc ttgcacaggc
     2461 aatccattga attgcaatcc gtctttaaaa tttttcgttc ctaagaccgt ttcattacct
     2521 gtggctttta ctaaaacacc ctccacacca tcaattcctt tggcatgagt tttcaaatac
     2581 ttagcaactc cgtcttcttt taattgcaca atatcagcca ttaaaccgtc cccactttct
     2641 caaatgtaat attggctaat ccatcgagtt tcactttatc ttctttggac atcaaaccat
     2701 ttgctgtcgt cgtagccaca gctgtcgttg ttgcattggc tccaggatcg cctttgtccc
     2761 ctttatcacc ttttggcaat acaaaattaa actttgctgc acttgcagta ccagcatttg
     2821 tgacactagc tattgtacca ctcgttactg tccctacagt aattgtggca gcctgtccag
     2881 ggtctccttt atctcccttg atagtttcgg gtttaccctc aacagcatcc caatgggttt
     2941 gagggtaaac ctgaacatca ttctgtttta cttttacaat atctgtcatt tgtctatacc
     3001 tcccctactt tttcaaacga tacatttggt attttggaat ctgtataagc ttgtgcattt
     3061 tgataagctt cactcatttt ttgatcaaca tattcttgag agacgccgcc accttctcca
     3121 ccacctgttg cagagattac tcctgtgttt gaaatactga tattagctcc ggcagtatag
     3181 ttgaattgct tatcggccga taaaacatca tcagtcatcg ttaaaccttc accgacacga
     3241 ataccacctt ttacaaattc ggaggcagtg ggtaaaacgt aaaaaccagt cacttctgca
     3301 tttatttttc catcttcatc aatagtgatg ttttctcctg cagtatagtt tttcagtgaa
     3361 tcgagttttt cttttaactc cagcgaaaaa ttgacatcag attgtctgac tgcagagact
     3421 tttcctgtct catcaatcgt caataattca ccaattttta tgccacccaa acggtcgata
     3481 gtggcaactg gcaacacgta gtcaccttct ccactgttta aaattttttg atacatttct
     3541 gacgtgataa tgccgtcatc ttcttcactt gcatagggta attctgtgag aacattctct
     3601 aatcccaaat cttctttagt aatgacgacg gctccaattt tgccattaac agataaaact
     3661 gttgcttctc ctgctattat ttcttctaac ccaagaactg cggaagcatg tgtgattgga
     3721 aagaattggc gttgtacacc tgttttttca tccgtttcca tcattcgttt agttttagcc
     3781 attagatcac cccaactttc tcaagagtga acacattttg ctttggatca tcaacggttg
     3841 cgatgatcaa ggctccttct tcaatcggga attctacagt tccaactttt tcgacttcgt
     3901 gattatctga aaagagatca tcctgcaaaa tatcagtcac ttcaatctca ccgtattcaa
     3961 tcgtgaaaag tgttgctcgt aatttctgat atagataatc catatctgcc agcaagcgct
     4021 ctgaaataga cgcatgacga actccttgaa tatcaacacg tgcatccatt aattcggcta
     4081 acattactcc acctggatcg atagacttca aaatatcttt gattgattca aaccatttta
     4141 aataatcggt ttcttgtcct tctctccatg cttcaaatgt attttgttga tcttgtcgcc
     4201 attgttcaaa ttcttttttg cgtgcattca tccaatctgt gaagtcgcct ttgttttcat
     4261 tgataaaagc tgtcatatcc gcaattaaat cttcgactgt ctgccaatac gatcccattt
     4321 caccctctgt ttttgagacg gctttaatca caaaatagga gaagtcttgc gtcgtagcaa
     4381 ttaattcatc atctctataa atgataaaat ctgctgtttg gcgatgtagg cattgcatcg
     4441 aatattgatc aaatgtgtac tgaatttttc ctttttttgc atcaatgact ttagtaccta
     4501 attgaactgg gtatttgtct ccgacaactg attcaaaata gactttacaa ccagttagat
     4561 catagggcat tccattttca gtgatggttg cttccataac ttcggaattc ttattgcctt
     4621 gccgtacctg aatcattccg acatagttat aaggctcagt tgtgctgagt actacgttcc
     4681 actttgccat taattaccac tcctttctca aaatcaggcg ggatacaaat agaggctatc
     4741 tgaccagatc cgaagaattc acggtcatat tctgccacaa ttccacctga ttctgcattt
     4801 tgttcatagg tttgaatacg cccattttcc agacctttga ttacacctgt gtggccgtaa
     4861 tattcatggc tttcaaatgt atcactgatt tttgcgtttc tttcccaatt gataattgag
     4921 ccaacaacta gctggtcata ctcaggatga gcgatgaccg tccataaata taatgaccat
     4981 ggataggctc gtccaatttc agcagcggaa aatacattgc catgacgtac tttaatctcg
     5041 tattgtgtac ctgcccccat gtcaggacca atcatcacac cggcatattc cgcagataat
     5101 gcataacatt gatggtttcc gataggttga ttcaacaaac ttttcaaatg atttaatcct
     5161 ttttcttcga tcacaaaatc acctctattt cttcgctctg gcgagacaaa catcgccgcc
     5221 atttaataaa tacccgaagg accaatcgac gcgaccgata ccaacaccat tggaattcat
     5281 gccacccatt tcaacaatag aagtgctata tcccttccaa tcctctgcta aaatagcggt
     5341 atgtccatca ttaccagcac cagcacctaa attaacaatg atgatatctc cggctttcgc
     5401 ttcatttgga gatatttcag tcagatactg acgtgcccct cttgcatctg cggccattga
     5461 acctgtgtac cacagagtcc cacgtgttgc ggttttatat cctgcttttg tcaaagccaa
     5521 ccacacaaaa gaagagcagt cagcataccc attacgatca ggattctcga cgctaccaaa
     5581 attccaacgt aacggttgcg agtaatggaa ataacccatc aagcttttgg ctgtactgag
     5641 aatactgcca gcagatggga cttgaagatc tttgaatttg ttataccaat taactgccca
     5701 tccttgtctt tctggatgag ttgtggctgg tctttcatag tttcgttcaa atgtataagc
     5761 agcagctgct ggatcagtca ttgctttaaa tccagaaaca cttgccggtt gaacaattcc
     5821 taaccattga ccattataca ttgtccaatc taataacttt ccttgagctg acattgttcg
     5881 ataatcttct gtaattcctg cagctctcat caggttttga acataagtcc tgccatccca
     5941 tgttgctgga ggaaccaaag gatatgctga accatcccat tgaacgattc cataggctgg
     6001 accgccaact tgtgcaatat ctggattcat tgcagcaccg gcttctcctt gaacatttcc
     6061 aaggattcca gcgatggatg ctttggaata ccctctagaa agaagcatga cccataattc
     6121 ccatgcaaat ttgtctgctt gactggtaac ctctggtggg tacatgccat tccaactacc
     6181 gccatcatta tttccacttc cgccttgtcc agggaatact tcttgtccgt taattgtgag
     6241 ttttccgcga atattgacgt ttttcataaa ttccgcatca ccaaaggcat ttagcttttc
     6301 ctgtaggttt aaacttccaa ataatataga agttccattc ccaagtatta cccaaccttt
     6361 tccttctttt ggtgaaatga gaatatattt tccatcgcca tttgttcgaa tgactaatga
     6421 attatcttct aacggtgtag gagtggatgc gccaggaaaa ggattgcctg cagaatcagt
     6481 agtaccaatt gtgccgatag attccttggt gttccagaac tccattcctt tcttggttag
     6541 ttccatgatt tttttattat cattccaaat ttgtaacgta cctttgacaa tttttagcac
     6601 atcgcccaga tcattaattg agctttcaag aacttcggca ttaataatcc caactttaat
     6661 gaagttagct acgatttcac ccttcgacgt cattgcgatg tcaaacgggc catttactcc
     6721 gttgtctgaa taacctaagc catttaaatt ccaacgccac acacgcttcg cattcgctac
     6781 attgggagta tccataataa gaatctctga tggagctttt tctggacgaa aaacgacatg
     6841 cccaccagaa ttaccagtga tccatgccgt cgcattcaac acattttgaa ctaacgtttc
     6901 tgttcgattg tcgattttct ttttcaattc ttgtgcttga gtatttactg ccgaagtgta
     6961 cagtgataaa tcattaccta aaacaatatc cttatatttc ccaagcgttg ggaaataagt
     7021 gtattcgacc atacgctctt ttatctcgat gtctagctct ttcgccctaa catgagcgac
     7081 atcaccaaag tgtaaagacg ctagctcttg atacatctca ccgtattcga gtgtgtgttc
     7141 caacgccacc atgctgactg tatgagtagc ttttggctcg tgaatacggt cattgtcaaa
     7201 taaggttttg ccccatttga tcaattcatc aactgtttta caatcaccat tttcacgttt
     7261 ggcaatacgt cgattctcat tggtcactcc atcaatttct aaataaccat attcgatagg
     7321 ttctttgtct tcatcataat cgttatcagg aaccccgcca atcaaataca ggctatttac
     7381 gatggattct tcatctattt cttcttcgat tgcttctaaa ttgataccaa aatcgattcg
     7441 gaaaccatta tctgatccaa tctgttttac cagtttcaaa tcatagttat ccatatcaag
     7501 ttccgcgcta gtcacgccag tcaaattttg gttaccgttg tttgaaccaa tgattgcttc
     7561 aactggcgcg acttgtcttg ctgtaaattg gtgcgttgtc cctacattcg aaagatagtt
     7621 aaatttttga tcaaatgcca aactgttttt aagattggtc atgatctgag aaccgttgcc
     7681 gttatccgtg aatgacttaa cgataaagtt tttattggcc ataaatccaa tatgacgagc
     7741 agttacagaa acagacgtca aattcttttt gatattgtaa atttcgaagt attgccatga
     7801 tccatctggc accttcgctt ttatgaaatt tccttttttt aagtaagacc gatactgtcc
     7861 gcttcttgaa tagttaccat aaaaacgata ttggccattc aacacacgat tgatttctgg
     7921 caaatcttcc cagtcaacta acgacgctcc gttcacgctc aaatcatctg gcattttttt
     7981 ataagcataa ataaattctt gcgtcacaga tacacactcc tattccaaaa ccgcacttct
     8041 ttaaattttc cggatatttt tacctgattc cattcaggct gcaatactgg ccaatcacca
     8101 cgcgtaaata aattcaaacc ttcttgtatt gcttttccaa gctgagtatc aaccacgatt
     8161 gttgcagcta acgtattaag tattgttagg cttttatcgc cgatagcaat cgtaatatct
     8221 cctccgttcg attctatttc cagataggga tgtgcaattt cgtcaccatg atcaaaaata
     8281 tccataacac tagaatgaaa aattttaggc gcttcaccga tttttctttt gagtggctga
     8341 caacgaaaag taacatcgaa tgtataaaag aagccccact cgttttcgaa tgggacttct
     8401 ttatccatgc tgcagatagc atctagatac ttgtctggat cattatgagt gattaattgg
     8461 cttttaccag taagccatct tttgacttcc ctcagtttgg aatgtgggat agtgatccct
     8521 tctatttcta aatcaaaagg ttcataatca tcaaacgtct ccgtcaattc tccgcttcgg
     8581 ccctgaaccg tgtaagtttc gtatcgcttg ttcggcataa tatctggaag ctctacctca
     8641 atgatgcaat ccatatcaat tactgcattc cgatttttcc aaataaaatt tggttcatct
     8701 ggattcataa attgtcgact caaataggaa caccccccaa atcataaata gcctgttggt
     8761 ttgctttagc aaatttacga ttcatacgat ctaactcaga tggattgttt gcatcgactt
     8821 taccaatgtg tatatgctgt tcaatgctac ctccggaaac tcttccgctg attcctttgc
     8881 gtttttcttc gtcagataat ggggtaactg ttgtttttcc attttttgca gtcagtaact
     8941 ctggaccagc ttcaccaacg attgcttgac cattgattag atgaccgcct tgtgctaaat
     9001 aaggcagttt tgaaatagaa aatgattttc cacctacacc aggaacccat tttggtatct
     9061 ttatattatt caatccacca ataaatccat tgatcaatcc aatcattcca ttgaatggtg
     9121 cttttgccat ggctgcaatt ccatcaaaga taccaccgaa aatgtttaca acgccgttcc
     9181 aagctcgttc ccaatcacct gtaaatatgc cagttataaa atctataaat ccggtgaaaa
     9241 ttctcttgcc tgcatcgaaa aagttttgga aattttgtat gattcctcca aaaatattgc
     9301 taatatatcc gcccataaag ttaaacactt ctaccgctat atcagaaacg ccttggaaaa
     9361 aggcattcac accgttccta aaccattcaa cattgttata agcccaaact agcccagcga
     9421 ctaatgcggc tattgctatt acagctacgg caaaccatcc gccagataat ccgaataatt
     9481 tgcctagccc actccacaca gtggccagat ctttaacccc agcggtgatc ttagtaacgg
     9541 aactcataag cgttcctatg acaaccaaag ctggtccgat tactgcagct ataccagcga
     9601 gtgtaataat ccagttctta gtaccttgat ccagagaatc ccaccaacct ttaaaacttt
     9661 tcaaagcatc gatcgccatt tcaaagaaag gcaataagct tatttggacg gattcgccta
     9721 catcagctaa agctagtttc gcattgttca ttgcttggtt agacttatca attggatcta
     9781 gcgtggcttc aaatgtttct ccaaccgaac cttgactttc atcggcgatt tttgccaagt
     9841 cttctaggtt taacgttcct ctacgaattg catctgccat tcttggacca cctttggtac
     9901 caaaaacttc tgcagcagcg ttgattgcat ctgtttcaga gcttgcattt ttgattttgt
     9961 cttgaagctc acccattcct tgagaaagag tcttcccatc ttttgcgtaa gcaacagtag
    10021 ctttcgatag actacttaat gctgcaccag aatcaacacc agcttgttcg aattgcccca
    10081 ttaaagtgac accttcgttg aaacttagtc ctaattgctt gatctgtggc gcaccgtcaa
    10141 ttgctttctg cattaaatca tcaactgatt gaccagtatt ttgagaggtt ttagttgtaa
    10201 catccagtac agaatttaaa tcgtcatatt ccagaccgta tgcttcgatt gcttgtcgag
    10261 ccattacggc tgattgagag acatcagtgt cgttaatctc agcatattga aggagataat
    10321 ttgttgagtc ttccaacttt ttatccataa atccaaactg agtattcacc tcaccgatcg
    10381 cttcaccgac cgtttgtaat ggtaaatgag tgtttgctcc tacttttttg aacgactccc
    10441 ctaatgaatc agctactttg ccggtagcgc cagttttcgt aataatcgta tctagggctt
    10501 cgtcgacttc gccgaatgca gcaagcgacg cagctccagc cgctacaata ggcgcggtta
    10561 ctccaacaga cattttttca ccaacgcctt ttactttctc acctgtttct tcgattttct
    10621 ggagtttttt tgctgtatca acagaaacgt caccttgttc tttaagtgca tcgttcgtct
    10681 gttctaatgc tgtacgaagt ttattttctc cggtttcaga attgagtaat tgtttataaa
    10741 gtttttctga ttcagcagaa tattcaccgg tttctttgac agatttttca tattcctctc
    10801 tcagcatttg agatcgttgt tcagccaatc ctaattgttt ttctaatttt tgtttagccg
    10861 ctcgcaattt ttctgtttga gttgcatcct tgtccatcgc agacacttgg tttttgtact
    10921 cggcagcggc taagttcatt tctttgttga tttctttgat tgttttcgaa taactgactt
    10981 cgccattcgt tttaaaatta agaacgacat cagattcttt accagccatt tatcttagcg
    11041 ctcctttcta ccaccaaggc gatttatcca tagttacaga ttgaggtggt tcaaaatcgg
    11101 tgttttgttg taaccactgt aaataagatt tgagccacaa gttaggtgta gacttcaaaa
    11161 agaaactctc actccaattc aatagagtga gagcaacgta aatataaaaa ctccaaggag
    11221 ttcctatctc ttccgattct ttttgtttac ctttcttttt gcttgcgttt tttgaaagtc
    11281 ttgtggcttc ttggattttt ttaggtcttc cacctgaaat gtctggctag caaaaatttc
    11341 catacaggta ctataagcag acaacacctc tccactcatt cccaaaaatt tgaaaatagt
    11401 ttctggatct tcctctaaac caccagtacg cagcatggca taaattaagg cgcgcatgat
    11461 ttttaggtca ctaggcgata agttagcaga agaaatttgt ccttcttttt tagatagcat
    11521 ggcattcata tcagattcga attgcgaata atctccacca tacacatctg ctataaattc
    11581 catcgtttgc atagtaaatg aaatagggaa ttctgcgcct tgaataatga caacagcaga
    11641 attctttaaa tcatcaacat gaatcccata atcggcaagt ctggccatta tttgccacct
    11701 ccaactttag ccaatgtttc ccactgttct tcatcgtata caggttgagc aatgaattta
    11761 ttaaataatt cgagagaagc tccgtctcta ttcgaatcaa aacttgcata catcacatta
    11821 ttgtatttca gtccgatgga aacaaaattt gcagttacat cgtcgatttt tgtctcttct
    11881 tctgcagtgg catactcttc atcaattaca ttagatagtt gtgtgttagg ataccatacc
    11941 gcttttttac cgccattctc aatatttccg atgaatccaa aggcaaaata cggaaattct
    12001 cgtgcggtat ttttgctaaa cgttactcca cttttagcaa ctaatccttt tatttcatcc
    12061 attacttcaa tcggaatacc cacatgatcc aacgcaattt catgttttgt ttctcgcgat
    12121 acacgtcgga acattttact tgatgcccat ttctctaacg ctgtaccatt acctttgacg
    12181 ccgatttttg taccaatagg caaacgaaca acttcactat aagttggcgc agtcccaacg
    12241 gtatcaggcg ttgccatcat cgcaattaaa atgtcgtcga ggccttcaaa atataaagta
    12301 tcttgttttc ccaattaaaa tcacccttcc cataaatcaa gtattttctt tgtcataatc
    12361 tcttctattt tttctttgtt ttgttcatat gtcccacttg caaagtgttg ggctctttgg
    12421 ttagttgctc cattttcaac aaatcgccaa taaaaagcag tttcttcaaa ctggaccttc
    12481 actcgatcat catcgaccac aacttttatt tgttcgctca tatgcttctt tttaagcagg
    12541 gacttaggaa tattaggaag caactgctct aggtaaaact ttgcagcttc ttctaaagat
    12601 tccaatgata atttttttgg atcaactcta gaaagattcc ctaaataatc cgatatttca
    12661 gaaaaaccat tcttattact tggcattttc catacacctc acatatgtgt aatagttggt
    12721 tacggtatcg tcgttctcat caccttgtat acctgtaaaa tctgaatatg gaataccagc
    12781 attttgtaac gcttcttcaa taacagctaa atcctgttct gttccaagtg tgaaaaaaga
    12841 aatttgataa tacggtaatt ttttatagac tttaccggat gccatttttt tactgttact
    12901 cacatttgag tacacgatgt atggatacat cgtccctaat ctggctttat ctctgaacac
    12961 tggcaacttt gttgatttca gcgctgtttt caattcatca aagctaatcg acataagcta
    13021 aactcaactc catttctctt gtatcaggat tcgtataaat gcgagtaatg ttatacgtta
    13081 cagaatcaat tttgagcgca cttaatttct ctgtgatgga tttatcccat ctgactttaa
    13141 ttcgtctgac aacgtctgtc ttggcttgct gtgataaata tttttcttga gaagtcacac
    13201 cgagttcttc atagaatatt agacgcttgg attcataaac cgtagttgga cgatcgttct
    13261 catctgttcc tgttttgatt tctagcaatt cagctttcca tcgcagatta ttggtctgtc
    13321 gtttcggcat tttgaatcac tccttgtaca ataaatggcg taatagcgct caaagctttg
    13381 tctaactcat cttcagatac tcggtattcg taggcaatgc cggcaaccat caaaataaga
    13441 tattcttgtt ggccaccagt tgctgttttg acataatctt ttgccatatt taaataaaaa
    13501 gagagcaaag aatcatccat gccctcttca aaatgaatat gtgatttgaa tttttcctct
    13561 aaagacaatt ctttagtttg ctcttccatc ttaaccacca actggttttg taatttcgta
    13621 gcgatatact gccggttcaa atggagaata aaccaattga ccatctagca agttgtaaat
    13681 ttggaatcca atttgatttt taccagagaa tttttcaaca agtttttgaa tttccaaggc
    13741 accaataact tcttgaattt taaatgcaga aaaatcgcca aaatataaaa ctggtgtgtc
    13801 tggtttaccc tttttatctg ctgcatctgt ccaatccaca ggatagccaa ctaattggta
    13861 acctattcca ccttctgctt gtgtaaatgg acgcaacaaa ggaaaaccat catctgtttt
    13921 cattttttca atagcagtca aagcagctcg attaataata aagcgtccct ttttcatcac
    13981 ttctgtcact ggtgtatttt taaattcgat taatgcatca tataattttt gcccagcacc
    14041 tgcagcagtt agatctaaag gtttttcaaa tgctacagcc tttttggcta atgcaccagg
    14101 attttcattt ccagcgtcat caccattgaa catataattg atttctttac gcacataagc
    14161 ttttttcaat tcttccacaa caatatcttc aactggaaca ccagacattt ttagtaattt
    14221 tttagttact gttgccaaag catcgaattc ggcaggatca agcaaaattt catcaaactg
    14281 aatagctgtt tcagcaatat cagttgaacg ctctttcttg tttacattcg catctgcttt
    14341 cttcacaaga attggatatt tgacatctcc tgatgttcgc accactgttc cgtatttacg
    14401 aagtaaattt tcttcttgag cataagtaat aacttcagat gcaattactt ctgggacagt
    14461 aactgaaccg ttgccagctt caatccctaa agctcgagct tctgcttcag aaatatttcc
    14521 aactacaaaa ttagcaaatg cttttcgtag ttgttgatct ttttgtttat tggtcatttt
    14581 tgcacgtgcc tccaatccgt tcttaattga tccaagtaat ccatctcgtt gctgttgagt
    14641 aatcattcca gaacgatttt ctgaactatc ttcactgtct gaatcttctt cttgatcgtt
    14701 gtcttcacca gaacggcttt cgtctgaatc cgtactattc gattgatcat ctgtattgtc
    14761 gtctgtttca tcagttccag aatctgcgcc taattcgtct ttaattccgt tcaattcatc
    14821 aataacactg tcaatttctt ctttcacggc ttctaaatct gcttcacgta cttcaccaga
    14881 ttcgattcgt ccgcgtaatt ccgttaagcg ttgctcatga cgagcttgta attttttcaa
    14941 taattttttg ttcatggtgt atcctcctat gcgttaagcg ccccgttaat tttttgaatc
    15001 atttttttac gtgttgcaat gtcttgctgg acttcctgtt tgcttctagc aagtgctgct
    15061 tctgtatcct gataggcagg aagagaaacg attgatactt cgaataattc cacttcattc
    15121 aacgtacgca aaacaggatc ggtgttgtaa tcccacgttt cttctgttgg aataaaaccg
    15181 aagctacatt gatcaatatc tccacgactc atagattcga taaggttatt agcatcggtt
    15241 gtgttgggta actcaacttc aaatttcaac ccacgttcgt cttcttcaag ccgaagtgtt
    15301 ccactttttg ttcgtcctag aactttcccc cagtcatgat caaatagaca tcggacgtcc
    15361 gaatttgata atgccttcga aaatgcacca ggagaaatta cttcttctaa gccttcccaa
    15421 agtaacgttc gactgttgaa cacagcagca tacccagtta cgattcgact attattttct
    15481 gtaacatctc tagtactcaa gttggtgata tcaaatgtcc gaatttcctt ctttttcatt
    15541 tccatcaccc cctttcacgt tttgatcatt cgttggtaag gaatcatctg ttgcattttt
    15601 ctggccaatc ctagacaagt catttgaaat atagatagct tgtgtttctg gagtgttctg
    15661 tttaggaaaa ccaagcattt ctgccacatt atctggactt gtaatcccag tacgaacaat
    15721 gttgtagcca atatttgttt ttgttgagta aggaacaaaa tccaaaatgt taattttcca
    15781 ttccactcga tagccagaat taggcataaa aaaaagagcg gtgtaatgtt cgctcttgtt
    15841 cttcaatatt ggtttgattg ctttgttgtg cagatacatc atcgcttttt cgatgtctgt
    15901 tttcatcaac gattgatacg tatcaacatt gattcctaaa aatttaccta agtctttttt
    15961 ataaacacct aaatagttca aaatagccgc gtcatcaaca ggacttttta acgtctcgat
    16021 ggaatatcct tttcccagag gaatcatctt aacagaatga tcactgtcat tttgcgttcc
    16081 ttccagttga tccaatatag ctttgacaat tttcgtttgg gcgctattat ttggattgat
    16141 gtgggcgtcc agtttaagca tgaacgcgag taaaccacct ttagtatatt tgtccgtcaa
    16201 aactttttca gcgctcagaa cgccttccag agtgtttctt gcaaggtcaa ttattccagc
    16261 accttttaat gaatcagttc cgatgttctt aatatgtcga atcatttgac caggtatttt
    16321 ttgtccattc atttcaaatt cttcttgaag tcgttcattg attttagtgg ttacaccgta
    16381 tgccaaatga agctggtccc gatctgttaa tgggaatgtc tcaccattga tcagtagggt
    16441 atttgtttcc aatttggtaa attcgaatcc ggtcaaataa tcattgggat tcttcaaaat
    16501 ttttagcaag tggtggtcct tcacttcatt accgtctgga cctatgacaa caggtgagga
    16561 caacgctacc tggtttgaga tatcctggac caattcataa acatcagaag attccatgat
    16621 agaggaatct gttacatatc tttgaccgta acgcgtatag tggccaaaca tatcctcgat
    16681 gtacccacgc ttttccataa aggaatagac tgcattcgat aaccgatcac gtaatttcaa
    16741 tatttctcac cgcctttcta tttatctata gatggaacta aggtaatcat ctaattcatc
    16801 cgaatcaata tctgtcatct gattcatcgt ttccttatga ccacacaaaa acgccacgaa
    16861 accatcaatc tttttctttg attgacgttt acttggcgct ttttgtccgt tgatgttagt
    16921 aattgctaca acattcaatg tgcaataaag gaacaatgga ttatcaaatt gaattcgttt
    16981 ctcataaaac aaccgttcga catcatcaaa aggagcgttc aacactttag gatattgggc
    17041 aacctcaacg cattccaatc ctaagttctc caatttctca acaagtttgt cgctcatcgc
    17101 tggatcataa ttcacttgtt gtatatcgta taaatccatg cagtcttcga tgtactgcaa
    17161 gatttgatct tgatcaatca ttttaccatc gcaaaattca acaaaacctt gttcagctaa
    17221 atcgctgtaa ggcacgttat cttctttttc tcgaaactct aaatcttcat tgggaataaa
    17281 ataaagctgc ttcactttaa ggaccgcttt tccatcttca tcccacgttg ggaagtttaa
    17341 agacacacag gtcaaatctc gtgtgcgtga taaatccaaa ccaatgtaac aaggttcacc
    17401 acttaaattt ccaagctctt gggtagtaac caaacacggc tctacttgat cctgttcaaa
    17461 gaaattatcc gcaccgttta caaatacatc caagtgcttc gttagaaact cggcttttga
    17521 atgtgctgaa cgttgtgccg ttttaaacgc tgactccaag gcggaaagat caactgatat
    17581 tccccagtta ggattgcaca tttcccaaac ttttctatcc gtccaatcat agtttttatt
    17641 tggttcatag attagaacaa agttcgaatc attatcatca cgtttcaaca cttcttttgc
    17701 ttcacgatat acgcgcattc caacagacga tgaacctttc ccagcagttg aaatattaaa
    17761 catcaatggt tgtggcaaag agatttgtgc agacttaaaa ttgtcatact gttccatttt
    17821 ctcttgctta tgcaattcat cattcaatac aaaatatgga ttagaaccct caatgttatc
    17881 gatattcttc gtttgaacaa taaatttatt cgaataagcc atatcttcat gaatataatc
    17941 ataagtaata ctagaaacag ttccctttgg tcctttgaat attttagttc cttcaagtag
    18001 gattggattg tttaatattg tagctgcaaa aggtttggca gcatattgag cttgagcaaa
    18061 gtcggaagcg cacgcataac aatcgactga caaagcacct tctccataca ttgcatagcc
    18121 taatgaaccg acagcaatca atgttttacc gttcttttta gggatttgga catacgcttc
    18181 acgagtaaca cggacaattt gccctttttc attttcttta acccatccat acatccacga
    18241 gtaaataaat ttttcccaag gttctaaaat gaatggcttt ccaaccatct caccttttgt
    18301 gtgaacaata aacgactcaa cccagtccat catttcattc gcacgatcta catcaaacca
    18361 aatatcttta cgttttttcc accgatacca acgatccact gccaaacgaa cagttttagg
    18421 atatttccca ggtttctttc ttacttcttt tgcaaataaa tcggcataat ttacaccagg
    18481 ttcgatcatt tttcagtacc tgccttctta cgccatttgt ttctgtgcgc tgctagttca
    18541 tctactggct tttcttctgg acgtgtaatt tcttcatctt ttcttgcagt cgatccgcca
    18601 gttatttgtc taccagtttt agccttattc gttagcccca acaaatctag agctttcatt
    18661 tttttatctg cccaagtttc tacttgctgc gccaatggat gcttcgattg attagtggcc
    18721 cctgatttat tcgtgaattt ttgcgtctcc ggaaagcctt tttccttcca caaaagatat
    18781 ttgtgttggt aaatttcaaa aatatccaaa tatgattcga ttaatggatc aagagtgatg
    18841 gtgtacaaat cagacaaatt cattattttt aaaatacgag ctttttcagc acttactttt
    18901 tcatcaacaa tcgctttacg ttgcgcttta gtcgtcatac ttgtatacac ccccctttgt
    18961 tttttgaaaa atttgaccta acgatgcgcg tgactccccg ctaccctatc tccccacgcg
    19021 aaaaaatttt gaaaatggat aggggggctt caattgaaat acgaagggaa aacttttttg
    19081 tcttccgttt cattttcaac aattggatga cattttgaac ataaaagcat gagattgttt
    19141 ggatcaagct taagcagttc attgtctttg atgggtacaa tgtgatggac atgtgccctc
    19201 ttgccgaaga taaactgacc gcatcgctga cagtgaccac cttctctttc ataaataaat
    19261 tgacgcatat ccttccatgc ctgtgttcga tagaatggtt tgttctcatg atgataaaca
    19321 gactttgctt gttgcttctt cttacgtgat ttgcttgatc gcttgtgttc agcacaatag
    19381 atcccctttg ctattttgtt cgtgcatccg tcaaactgac agtacttcat tctgcttcac
    19441 gaataagatt tatgatatcg ccttttgcac ggacagcacc aggaatatca atgccatgtt
    19501 tcttagcata tgcacgtaat tcttttgcag tcatattgtc cagttcatcc gtatcttcag
    19561 tggactgatc attagtagct tcgtctccat caaaatcagc agcagtgttt ccatcgctat
    19621 caagaatttc actgctatta gtaataagtt caccattaac agccacaaac gttttaccat
    19681 cacttaatat tcctttttct tccgttgctt caaaatcagg ttcttgacct ttcggaacta
    19741 cgacagtctt tttcttttct gaatcccaat actctgttcc tgtgatggtt gttctaattt
    19801 tgatcattgc cattttgatt ctctcctttg taatttgtgc taattacttt tgcacccatt
    19861 cgttcatacc attcaacagt ttcctgcaaa ttgggtaaag tgtgggataa taaagaaata
    19921 accaacacat ccattgctat cccacgatta tctttatcta cttgcacatt tgcataggtg
    19981 ccgttccaat tgtttcgtaa accagtagga ctgttctctg cttctattgc tttcttatag
    20041 acatctccca tattcgttgg gacattcgcc ataagcattg cctcatgaaa atcattcata
    20101 gataaaactc ctttcaaaat aaaaagacca ctcaaagagt gatctaatat gtaatgcaca
    20161 ggcagggacg tttccgatcc tgtgcttgag tcatttgacg attcatttgt accgaatccc
    20221 aaaactcaat ctaacctaac caattatgta atagcaacct acaccgattc catcgattac
    20281 tatcgacctc gccatgctcg tgtactttga gcgcccattt ccaaccctcg gttgctaacg
    20341 aatactgaaa cagaataccg tctctgttca tgaccaacgc gacagtacaa agctttgtcg
    20401 caagcaggtt atcgtattcc taataacccc aactaatgtt tctctcctaa cctacacctg
    20461 agagtggcgc acgtctgcgc tagtatttta tgccttctaa ctgctagtag catcccgact
    20521 agtcagccaa ttcatcggca tccaataaga tgaattgttt ccgtaggttc cttaagccac
    20581 tggcaaggaa tcgaaccttg catggattac tctcattcat cctttattgc gtcttctact
    20641 tccgccacag cgacaaatta atattgtgaa aataaatact aagcgtataa ttttagttat
    20701 cagcgagtgg tccgctgaaa taaatatacg aaggtgaaaa ctatgttaac caatgaacaa
    20761 cgcgctcatg atttagcaat tgcaacatta ccttttgtca gagatattgt taaatctcaa
    20821 ataattgaag ggaaagatgc aaaatttgat gcatattttg aatatataaa gctttataat
    20881 caat
//
LOCUS       DAGCWU010000033        20732 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.033, whole genome shotgun sequence.
ACCESSION   DAGCWU010000033 DAGCWU010000000
VERSION     DAGCWU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.033"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            378..1067
                     /locus_tag="MBZ71_000976"
     CDS             378..1067
                     /locus_tag="MBZ71_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4428947.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1054..2262
                     /locus_tag="MBZ71_000977"
     CDS             1054..2262
                     /locus_tag="MBZ71_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4428948.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2556..3827
                     /gene="serS"
                     /locus_tag="MBZ71_000978"
     CDS             2556..3827
                     /gene="serS"
                     /locus_tag="MBZ71_000978"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBT4428949.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4376..5860)
                     /gene="guaB"
                     /locus_tag="MBZ71_000979"
     CDS             complement(4376..5860)
                     /gene="guaB"
                     /locus_tag="MBZ71_000979"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBT4428950.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6365..7060)
                     /locus_tag="MBZ71_000980"
     CDS             complement(6365..7060)
                     /locus_tag="MBZ71_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBT4428951.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7150..8250)
                     /gene="ychF"
                     /locus_tag="MBZ71_000981"
     CDS             complement(7150..8250)
                     /gene="ychF"
                     /locus_tag="MBZ71_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBT4428952.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8259..8450)
                     /locus_tag="MBZ71_000982"
     CDS             complement(8259..8450)
                     /locus_tag="MBZ71_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBT4428953.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8471..9361)
                     /locus_tag="MBZ71_000983"
     CDS             complement(8471..9361)
                     /locus_tag="MBZ71_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBT4428954.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9348..10115)
                     /locus_tag="MBZ71_000984"
     CDS             complement(9348..10115)
                     /locus_tag="MBZ71_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4428955.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10127..10840)
                     /gene="rsmG"
                     /locus_tag="MBZ71_000985"
     CDS             complement(10127..10840)
                     /gene="rsmG"
                     /locus_tag="MBZ71_000985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBT4428956.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11072..12973)
                     /gene="mnmG"
                     /locus_tag="MBZ71_000986"
     CDS             complement(11072..12973)
                     /gene="mnmG"
                     /locus_tag="MBZ71_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBT4428957.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12990..14387)
                     /gene="mnmE"
                     /locus_tag="MBZ71_000987"
     CDS             complement(12990..14387)
                     /gene="mnmE"
                     /locus_tag="MBZ71_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBT4428958.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14686..15069
                     /locus_tag="MBZ71_000988"
     CDS             14686..15069
                     /locus_tag="MBZ71_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428959.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15237..15995)
                     /locus_tag="MBZ71_000989"
     CDS             complement(15237..15995)
                     /locus_tag="MBZ71_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBT4428960.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16013..16831)
                     /locus_tag="MBZ71_000990"
     CDS             complement(16013..16831)
                     /locus_tag="MBZ71_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBT4428961.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16828..17184)
                     /gene="rnpA"
                     /locus_tag="MBZ71_000991"
     CDS             complement(16828..17184)
                     /gene="rnpA"
                     /locus_tag="MBZ71_000991"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBT4428962.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17302..17436)
                     /gene="rpmH"
                     /locus_tag="MBZ71_000992"
     CDS             complement(17302..17436)
                     /gene="rpmH"
                     /locus_tag="MBZ71_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBT4428963.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17959..19293
                     /gene="dnaA"
                     /locus_tag="MBZ71_000993"
     CDS             17959..19293
                     /gene="dnaA"
                     /locus_tag="MBZ71_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBT4428964.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19491..20621
                     /gene="dnaN"
                     /locus_tag="MBZ71_000994"
     CDS             19491..20621
                     /gene="dnaN"
                     /locus_tag="MBZ71_000994"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBT4428965.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5938 a         4225 c         3803 g         6766 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcatttc caacatgtca acaacttatt ttttattatt
       61 taataagtta ttggtgttct tttgaacaaa taataatata tcaacatcac atgtgaaagt
      121 caataagaaa atgcttattt atcaacattt ctagcttaca tgatcattgg ttagttctgg
      181 tcctttttcg ttcgtcttta taactccgag agacgtaaaa tccttttgtt ttcagtttaa
      241 tgttcatgtt cttgtcttat tcacaccaac tagatgtctc accttctaaa attttcctta
      301 acaaagtgct gcttttatct atttctgctt ttttttgcta gaataatagt aacaatatga
      361 ctctggagga tttatttatg aaaattttga ttgctgacga tgataaagaa atcgttgaat
      421 tattaagtat ctatgttcac aatgaggggt atgaagcagt aaaggcttat gatgggaaag
      481 aagcattatc taaaatcaga acaatccctg atattgaatt acttatttta gatatcatga
      541 tgcctgagat ggatgggatg caagtagtga aagagctacg aaaagaatcc caaataccaa
      601 tcattatgct gacagcaaaa actactgata tggataagat caaaggactg gtcgctggtg
      661 cagatgatta tgtgaccaag ccatttaatc cgttagaagt aatggctaga gtaaagtctt
      721 tattacggcg tacaaaaatg cagattgccc cagatcagcc tgatgtatta gaagtaaatg
      781 cattaatcat caataaagac tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt
      841 taacagcttt ggaattcggt atcctctact tgcttgctag tcatcctaat cgagtattta
      901 gcgcggatga aatttttgag cgtgtttgga aacaagaaag tattgtctct gctaaaactg
      961 tcatggttca cgttagtcat ttaagagata agattgaaga agcaactggt ggtgaaaaag
     1021 tcattcaaac cgtttgggga gtgggctata aaattgacgc aagatagtaa acgagcaact
     1081 gaaaaacgcc gtcggatcaa tttgacttca aaagaaatca gcgaattgtt agcggaagga
     1141 atcatcacga ttatcctttt gcttctatta aatgtttcta ttctggttgt tatcagttcg
     1201 gtgatcaaca gttcaccttc tttgacgaat gcgatttggg attcaaagaa tatttttgca
     1261 gaacgattga acactgattt gttctggaac ggccggaatt ttattattcc ctttttcttt
     1321 ctattagata ttggtgtttt gtattggcgc ttgattcgaa gatatcgcca aatgcaattg
     1381 cggcatatca tcagcgaact tcactacatc gcaaacggaa attatgatca tcgtatccct
     1441 tttgaactga gtggtgactt aagccgagtg gtaacaagta tcaatggatt ggtggatagt
     1501 accgtagcag cgattgaaga cgaacgtaaa atcgaaaaat caaaagatga attgatcacg
     1561 aatgtcagtc atgatatccg taccccactc acgtccatca ttggatacct aggactgata
     1621 gaagatggcc agtatcactc agaagaagat cttttaaagt atacgcatac tgcttatatc
     1681 aaagcaaagc agatgaaatc tttagtggac gacttatttg aatacactaa ggtacgccag
     1741 cctgctgtcc cggtgaattt ttctgccttt gacatgatcc agttgatcga acagttagca
     1801 gctgattttg agttggaagc ttctaaaaaa aatatccaaa tacttgttca atcaaaagta
     1861 gactccctta tcatggatgg cgataccgaa aaacttgtac gtgtcttcaa taatcttttg
     1921 acgaatgcgt tgaaatacgg aaaaggagca acgaagatcg taatcgaagt agaacgtatc
     1981 ggttcagaag ttgttgcaac agtcaaaaat aacggagcaa tgatcccaca acaggcaatc
     2041 gataatctat tcgaccgttt ttatcgtgta gaagaatctc gttcacaagc aactggagga
     2101 actggacttg gtttagcaat cgctcaaagc attgtggctt tgcacggcgg atacatttat
     2161 gcaaaatcag ataagcaatg gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa
     2221 aaattaccta ttgatacaca ggaagttatt gacgaaagct gaaatattcg ctattcttat
     2281 tacaattatt agaataaatg aaaaacacag aacccagtac actttattct ttttcagaga
     2341 gctgatggca ggtgtaaatc agcaaaggat aatttggaag ttccagttcg tttacgtgcc
     2401 ctttcgaaat aagaaaggtc ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg
     2461 ctgaacttgg gtggtaccgc gaatttcaga ctgattcgtc ccaagaataa tgggatggat
     2521 cagtcttttt ttattttcaa aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac
     2581 aaaatattga agaagtacaa gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag
     2641 ctgaattcat ggaactagat gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa
     2701 aaaaatatcg aaatgatgtt tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg
     2761 cttctgctaa aatcgctgaa atgaaagaag tcggtggcaa catcaaagca ttggatactg
     2821 aaattgctga agtggatgaa aaattgaaac aaattgcaac tactttgcca aaccttcccc
     2881 atgattctgt gccagttgga acagatgaag aggacaatgt agaaatccgt cgttggagcg
     2941 aaccacgtac atttgctttt gaaccaaaac cacactggga aattgcagaa aatcttgata
     3001 ttttagattt tgaacgtggc gcaaaagtag ccggtagccg ttttgtctat tacaaaggat
     3061 taggtgctcg attagaacgt gctgtctaca acttcatgct tgatcagcat atttacgaac
     3121 acggctatac tgaaatgatc actccttata tcgtcaacag taaagcgatg tttggtacgg
     3181 gacaatttcc aaaattcaaa gaagatgttt tccaattgga gaatactgat ctaacattaa
     3241 tcccaacagc tgaagtacca ttaactaact attataatga tgaaatttta gatggtgcag
     3301 atttacctat ttatttcact gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg
     3361 gtcgggatac acgaggactg attcgtcttc accaattcaa taaagtagaa atggtcaaat
     3421 tttcggatgt agagcattct tacgaagaat tggaaaaaat gacagctgat gcagaagata
     3481 tcttgaaaaa attgcatttg ccttatcgtg tgatggcctt atcaactgga gacatgggct
     3541 tttctgcagc taaaacttat gacttagaag tatggatacc tgcacaagat acttatcgtg
     3601 aaatcagttc ttgttctaac tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc
     3661 gcgataatga tggaaaaatc cactatactc atactctgaa tggttcagga cttgcagttg
     3721 gccgtacagt agctgccatt ttggaaaact accaaaatga agatggttct gtcacagttc
     3781 cagaagcgct cgttccttat atggggaatc tgaaagttat taaataaaga ggctgtgaaa
     3841 aagccgctta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc
     3901 ataaattgga cttaataccg agaaactggc ttttgaacac catttatcga gaggttgtga
     3961 aatcagcgaa ttaacctgag tattagagaa aaaaatggaa aaatagtccc tcatatttta
     4021 ccgtttgttg aactaatacc gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt
     4081 aagcctaccc cccagctcct agttacaagg acaaattcaa aaaacagctc ttcatatttt
     4141 atgaatgttg tacttgtgat tcggaggtgg caagggaaca ctgtttattc aaaaagaggc
     4201 tgggacagaa gcgtttaact ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa
     4261 tttttgtgaa tttcttctta tttccgaagg agttgcttct acttccgccg tttatacgtg
     4321 tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctctttaa tgtttttaag
     4381 attcaactga atagtttggt gcttcttttg tgatttgtac atcatgaggg tgtgattctt
     4441 ttaatccact gccgctcatt tggataaatt gcgcttcatc acgtaattgc tgtagattgg
     4501 ctgccccaac ataacccatt cctgaacgca aaccgccgat catttggaaa ataatatctg
     4561 caacacttcc tttataagca acccgacctt cgattccttc tggtactaat ttatttgctt
     4621 cattgacact accttggaag taacggtctt ttgaaccttt ttccatcgcg cctaatgagc
     4681 ccatacctcg gtatgttttg aatcgacgtc cttggaagat ttcgaattca cctggtgatt
     4741 catctgtccc tgccaacata cttcctagca tcactgcatg tccgccggca gctaatgctt
     4801 tgacgatgtc accagaatat ttgatccctc cgtctgcaat aatcgctttg ccatattgac
     4861 gagcaactga tgctgcatca taaatagctg tgagctgagg tacacctaca ccggctacca
     4921 cacgtgtcgt acaaatcgat cctggtccga taccaacttt tacgacatct acacctgcat
     4981 catataaagc ttttgtcgct tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg
     5041 caaaagttga acggatttct tgaatttttc ggatcacacc tgcactatgt ccatgagctg
     5101 tatcgatgat gattgcatct gcccctgctt ctaataaagc attcgcacgc tcaaatgtat
     5161 cgcttgttac accaacggct gctgctacaa gcaatcgacc atgttcatct tttgctgcat
     5221 ttggaaattc gatcactttc tcgatgtctt tgatcgtgat caatccgctt aaacgacctt
     5281 cgttatctac gattggtaat ttttcgattt tatgtttttg caaaatcttt tctgcatctt
     5341 ttagagaggt acctactggg gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg
     5401 gcatgtgata atctgttaca aaacgcatgt cgcggtttgt aattatccct accaattttc
     5461 tgttttcaag tgtttcaacg attggcacac cactgatacg atatttgctc atcaattctt
     5521 ctgcgtctgc tacaagatgc tgtggcgtta agaagaacgg atcaatgatt acgccacttt
     5581 cagaacgttt gacttttctt acttcatccg cttgctggct gatcgtcatg tttttatgga
     5641 tcacacctag tccgccttgt cttgccatag caatggccat cttgctgtcg gtaacagtat
     5701 ccatacttgc actgatgatt ggaatattca acttaatatt tttagctaat tgtacgctca
     5761 tatccacttc gtttggtaac acgtgacttt ctgctggtat caataagaca tcatcaaaag
     5821 taaatccttt tttcgtgaac tttgtttccc agttggacat ttttagtacc actcctctaa
     5881 tttagtttat ctattaaaat aattgatttt ttcatttaag tcaatgatct acttggaatc
     5941 aagttagctt tctcatagaa taccaatata aagcgtttac atctgtgtat attaaaagag
     6001 attgtaaaaa agctgtcctg catcaagtaa taagaacagc caatcaaaaa cagcttctca
     6061 tgtttttgaa ttggttgggt ttattaccgc agatgcaaac ttttgaacac cgtttagtgg
     6121 agaggctgtg aaaaagctgc ttagtttcga gtagtaagaa aaattttgtg aaaatggctt
     6181 tccacatttt tgcataaatt ggacttaata ccgagaaact ggcttttgaa caccgtttat
     6241 cgtaaaaagg gactgtgaca agacttttgt cacagtccct tactattatt taactatgga
     6301 taaacaaaat gaggttcttt actaccccta gatatctagc aaaatagctg atctttttgc
     6361 tagtttatct tcttgggacg gataaacttc caataatccc gtattttttc ttcaagtagt
     6421 aacggattgc taaagcagtt gccccgataa tgataactac tactggatct aatactggat
     6481 tgataactgg tggcaatagt gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc
     6541 cacctaggat aagcaatgtc ttacccattc ctggtttctt gcttcgatct gctccgggat
     6601 attcatattg atagatgtat ttgtacatca tataaaagac ccagccaccg attgcagaac
     6661 ctagaacgag tgtagaaaag ccataagctg gcgttgttcc acgtgaaaac aatccggtaa
     6721 tcgcaatcat gatacttaat agaccaagca acagcaacgt attatctaac cacatcaata
     6781 aaggggtgga tgaatttttt acttctgctg gcttattgat gattgcttcc gttctttcag
     6841 agactgtccc aaacaattga cgggctgttc ttccgccttt ttgttctgca actaaagtag
     6901 gcagaatgtc atgaagggct tttacttttt cttcttcaga caaattcgct gcttctaaag
     6961 attttttcaa atcaaaaatg tactgttgat ttcttttggt taattttgtt tctaaactat
     7021 tgttttcttt tacgattgct cgtaattgtt ctggttccat tatctaatag tcctccttag
     7081 ccgttccttt tctcaacggt cgtcacaaaa gacattctac catattctac agctagtttg
     7141 atagcccttt tacacattaa agcggaataa cattacgtcg ccgtcttgta cgacataatc
     7201 ttttccttct aatcgaacac gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc
     7261 caagtcttca aaagagacag tttctgcacg gataaatccg cgttcgaaat ctgaatggat
     7321 gattcctgct gcttgtggtg ctttgattcc ttttttgaat gtccaagcac gtacttcttg
     7381 ttccccagca gtaaagtaag tagctaagcc taacaaatca taagcagcac ggatcaactg
     7441 atccaaacct gattcttcga ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc
     7501 taattctgcg atttcttctt ctgcacgagc actgaccacg atcacttctg cattttcttc
     7561 tgcagcaaag ttgcgcactt cttgtacata atgattcgcg tctgcatctg ctacgtcatc
     7621 ttctgagaca ttggctacat aaagaattgg ttttgtcgtc aataaaaata gtgatttaac
     7681 gattttttgt tcttcttcag taaattcgat cgttcgggca gattttcctt cttccagtac
     7741 aggtttgatt ttatccagta cagctagttc tgccactgca tctttatctt tggttttagc
     7801 aacttttgct acacgagtat agcgtttgtt gatagattct aagtctgcta acactagttc
     7861 caagttgatc gtatcaatat cttctagtgg atcaacacgt ccttctacgt gagtaatatt
     7921 atcatcatca aaacagcgaa caacgtgaca aatagcgtct acttggcgga tatggcttaa
     7981 gaattgattt cccaaacctt ctcctttact tgcgcctttt acgattccag caatatctgt
     8041 aaattcaaaa gtcgtcggaa cagttttttt cggatgtaca agttctgtta agcgttgcaa
     8101 acgccagtca ggtacttcta ccatccctac gttggggtcg atcgtcgcaa agggatagtt
     8161 tgctgcttct gctcctgctt ttgtgatcgc attaaacaaa gttgattttc ctacgttggg
     8221 caacccaaca attccagcag tcaatgccat aaatttattc actctttctc tgcatttttt
     8281 tcaattactt tttttaattt cttttcaaaa tctcgacggg tcatcatgac gatatgtccg
     8341 caattcatac atctgatttt gatatctgcg cccattcgaa tgacttccca gcgattcgtc
     8401 tggcaagcgt gaggtttttt catttcaacg atatcgtgca agtcgtacat aaaaatagcc
     8461 tcctttttgt ttattcttca tcaaattgaa tattcaagat atcaagaatt cgagtcaaat
     8521 ctttttgaga aagatattca atctcgatct tacctttccc atttttttct tggatagcta
     8581 cacttgtacc aaatttatcc atcaaacgat cttcgctttc tcggatataa tatggttttt
     8641 cctttgctag ccttgggatt tttttctttt cttttccatc attttgattt aactcagaaa
     8701 caagctgttc cagttggcgg acagttaaat tttcagttac tgcacgattg gctaatttca
     8761 agatcaatgt tttatttttt aaacctaaca gcgtacgggc ttgtcccata gataaacgct
     8821 gatcttgtac catttccttg acaagatccg gtaaagaaag caagcgcaaa taattggcga
     8881 tataaggtct gctctttcct aaacgactag ctacttctgc ttgtgttagt ttcaggtttt
     8941 tcatcagcat ctcatatgct tctgcttctt ctaacggatt cagatcttct cgctgcaagt
     9001 tttccagcac tgctacttgc atcatcgctt cttcgtcaaa gtctctaacg atagcaggga
     9061 tcgtttcttt ttcagctagt ttggacgctc gaaaacgtcg ctcccctgca ataatctcat
     9121 atccttttac tgcggaacgg cgtacgatga tgggttgaaa gacacctgat tgttcaatag
     9181 aattagctag ttcctgcaaa gatacttctt caaatgtttt acggggctga tacggatttg
     9241 gtcgcagctc attcaatggt agctggacaa cggtatcatc ttttacatcc accgtctcta
     9301 agttttctaa atcttgaaac agtgcatcga ttcctcggcc taatccttta cttttcttca
     9361 cgacttacca cttcctttgc caatgcttgg tagacttcag ctcctcgaga acgtggatcg
     9421 tagtcgatga tcggttttcc atgactaggt gcttctgata aacgaatatt tcttggaatg
     9481 atcgtttcgt aaactttttc gcggaagtat ttacgtactt cttctacgac ttcatttccc
     9541 agatttgttc gtgcatcata cattgtcagt aagactccct cgatctctaa ttctggatta
     9601 aagtgttttt ggactaaacg aaccgtattc agtaattggc ttaatccttc caatgcgtaa
     9661 tactcacact gaacaggtat caaaatcgaa tcacttgctg taaatgaatt gattgtcaga
     9721 tggccaagag aaggcgggca atcgatcaaa atataatcat actgactgct tacttctgct
     9781 aaagatcctt ttaatcttga ttctcttgcc atcattgacg tcaattcgat ctctgctcct
     9841 gctaattgga gagtcgcagg cacaatactc aaattttcgt gttcagtgat caaagttgcc
     9901 tcatcaattg gcaattcatt gactagaaca tcatagatgt ctcttgtcac atcaggtttt
     9961 ctgatcccga caccactggt agcatttcct tgtgcatcca tatccactag caatactttt
    10021 ttgccaaggc tagcaagaca agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca
    10081 cctttttggt tcgcaacaga tattatttgt gccatctctt ctcctcctat ttgattggct
    10141 gcttattagg cagtccgggc tttcttgggt atttctttgg ggttggtttc tttttttgaa
    10201 taacgatgat atgccgttga tcagagacat tcggcaaggt aaccgcctct tctttgagga
    10261 atttaccacc taataaagca atcgcttgtt ttgcttctgt caattcctct tcactttttg
    10321 ctgctttcaa tgcataaaaa tagccttctt gtttagccaa gggcaaacac aattccgcta
    10381 acacattcaa acgagcaacc gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag
    10441 gattttgccc aaatgtttct gcccggtcat gatataagga agcatcgatt cctaataatc
    10501 gggttaactc agataagaag gttattcgtt tattcagaga atcaacgatc gttatctgca
    10561 gttctggaaa ggcaattttt aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc
    10621 ataaagataa aggttgcgta aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat
    10681 gcttcagata tacgtctttt tcatccgtaa tagctgtaag gttcatcttt tcgttccatt
    10741 cgactaatgt ccgataataa atagcgaatt gattcatttg atgatcggta agctctatcc
    10801 ctttttctgc caaggattgg cgaaattctt ctggtatcat aaatattcct ttcattgtga
    10861 aacagcaatt gtttcacaga ttctctccct actttaacgc aaaatctcta gacatgcaat
    10921 ccttccatat ggattcttaa aaatttgaaa agtaagaaaa gccgatagac tttttctttc
    10981 tagtatgact cataaaatat acacagaaga gagaactagc tttgagcaat ttctgcaaaa
    11041 actagttctc tcttctattt attattacgt attacgctgg ttcactgact ttcgcaatct
    11101 tcccttgttc gatatagacc attaggatac ttaggtctgc agggtttacg ccactgattc
    11161 ggctagcttg ggcaattgtt tctggttgga ttttctgcaa tttttgtttt gcttctgttg
    11221 ctaatccgtt gattgctgcg tagtcgatgt tttctggaat ctttttcgct tccatgcgtt
    11281 ttaatttttc tactttttcc atggcttttt tgatgtagcc ttcatatttg atttggattt
    11341 ctacttgttc gatttctttt cgtgtcaatt cttcattttc tggaataaac cgtagaagtt
    11401 ctgcataagt aatctcagga cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg
    11461 gtgccgcatt aatcgttgct aaaaagtctt ggacttcttc tgttggtttg attcgtacat
    11521 tcatcaaacg tttgatttct tgttcaactg cttctttttt cttcaaataa ttcttgtatt
    11581 gatcttcttt tactaagcct acacggtgac ccatttctgt taatcgcaaa tctgcattat
    11641 catgacgtaa aagcagacgg tattcagcac gtgaagtcag caaacgataa ggttcgttcg
    11701 ttccttttgt taccaaatca tcgatcatga caccgatgta accgtcactt cgtttgatca
    11761 ctaaaggttc ttttccctga actttcaaag ctgcattgat accggcaatc agcccttgtc
    11821 cagccgcttc ttcataaccg cttgtaccat ttgtttgtcc agctgtataa agattttcaa
    11881 cgactttagt ttctaatgtc ggacggagct ggtgtggctc tactacgtca tattcgatag
    11941 catatcctgt acgcatcatt tctacttttt ctaatccagc aatagaatga agcatgttga
    12001 cttgaacatc ctctggtaaa gaagtagata aaccttgaac atagacttct tccgtattta
    12061 agccttctgg ttccaaaaat aattgatgac gtggtttgtc tgcaaaacgc acgatcttat
    12121 cttcgatgga tggacaatag cgagcgccta ccccttcaac gatccctgta aacattgggg
    12181 cacgatgtaa gttgtctcga ataatttggt gagtcgtctc acctgtatag gtcagccagc
    12241 aagattcctg atctagttta taggcactat ctggtgtgct gaagctaaaa tgattcggct
    12301 ctttgtcacc aggctgctct tctgtaacag aatagtcgat cgtacttgat tttactcgag
    12361 gcggtgttcc tgttttgaac cgttcgatag caaagccaag ttcttttaga tgattcgcta
    12421 gtccaataga aggttgagag ttattcggac ctgatgaata tttcaattca ccgataatga
    12481 tttctccgcg aagtgctgtt ccggctgtaa taacaactgc tttggcccgg tagctcgctc
    12541 ctgtggacgt cactactcca tgacaaacac catcttcgac gatcagttct tcgacgattc
    12601 cttgtcttag cgtcaaattt ggttcttttt cgatcgtatg tttcatttct gttgaataag
    12661 catgtttgtc tgcttgtgct cgcaatgctc ggacagctgg gccttttcct gtgttcaaca
    12721 tacgcatttg gatataggtt ttatcgatat tcttacccat ttctccgcct aacgcatcga
    12781 tttctcgaac gacaacgcct ttagctggtc cgcctacaga aggattacat ggcatgaatg
    12841 ctaccatgtc caaatttatt gtcagtaata atgtttttga tcccatacgt gcagcagcaa
    12901 gtgcagcttc tgaaccggcg tgtcctgcgc caacaacgat cacgtcatac gcatctgctt
    12961 cataatgttc caatcgtttg ttcctccttt tattttccta aacaaaattg actgaataat
    13021 tgagtgatta gctcatcttg tacactgtcg ccgacaactt ctcctagata gtcccagcaa
    13081 cgcgtcatat ctatttgaac gagatccaca ggcattcctg aatcaatacc tgcaatgact
    13141 tcttcaagtg agagggaagc tttttctaac aacgcgatat ggcgggtatt agaaacataa
    13201 gtagcgtctc tttctcctgt ttctccgctg aagaataaat cagaaatagc tgattccaaa
    13261 cgatctagtc cgtcgttttt agctacggaa attgaaaaaa ctggttcatt ttcgatcaat
    13321 ttcttcaatt tttcttgttc cagctgtgct ggcaaatctg ttttatttag aagaatgatc
    13381 cgtttcaatc cacttgtcgc ttccaataat tgttcatctt ctgctgtcag tggttcgctt
    13441 tggtttagaa caagtaagat caagtctgct tctgctaatg cttttcggct tctttcgacc
    13501 ccgatccgtt ccaccacatc ttctgtttcc cgaattccag ctgtatcgat tagcttgagc
    13561 ggtacccccc gaacattgac atactcttcg attacatctc gagtcgttcc tgcaatatcc
    13621 gtaacgattg ctttttcttc tcttaacaaa tggtttaaca aacttgattt gccgacattt
    13681 ggccggccga tgattgccgt acttaaccct tcccgcagaa ccttcccttg cttggatgta
    13741 gcaagtagcg cttggattct ttgttggatc atctgcgctt tttcaagcaa taacttcgta
    13801 gtcaattctt cgacatcatc atattctgga taatcaatat ttacttctac ttgtgctaat
    13861 gtctctaaaa tctcttgtct caatgagcgg atcaaagcag acagattgcc gtctaactga
    13921 ttaagagcta gtcccattgc tttgtctgtt tttgcccgaa tcaaatccat cacagcttct
    13981 gcctgtgaaa ggtcaacacg accattcaag aatgctcgtt ttgtgaattc gcctggctca
    14041 gctaatcttg caccttctct taacaatagc tgcaaaattt gattgactac aacgatccca
    14101 ccgtgacaat tgatttctac tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg
    14161 gagaccatga cttcatcgac aagctgattg ctcttgggat caacgatatg tccataatgg
    14221 atcgtatgac ttggtacttc tgataatcgt ttgtttccag attgataaac tttatctgct
    14281 agttgcacag cttgatcgcc acttaaacga acgatactga ttgcaccttc tccaggtggg
    14341 gtagaaattg ccgcaatcgt atcaaattct aaagtaattt cagccatttt ttcacttcct
    14401 ttttcatttt gcgcacaaaa aaagtgccca ctccaccctt tatgcacaat aaacggtgaa
    14461 cttcgcactt tgactgcttg ttcttgatta aattctggtc atagattatc acacttgcta
    14521 taaggaatca agtgtttaaa acaattagat gaagttgagc ctgttctgct tccattacct
    14581 tatatttaat tgtccttgga tgtctctttg cgttctttgg tttattcgaa gagaagatac
    14641 tgtataaaca aggctttatc tttcttaaaa aaaacggtac actaaatggt agcgctatct
    14701 aaactataca cttttgaaag gagaaaatat gtgagcccgt tcagatctgc tgcattggaa
    14761 gaagaaagtg accctcggat caacgatgtc gttttatcta cagggaatat caaccagaaa
    14821 tatatcgtcc gcgatatcgt ttttgttgtg gaatgcttag aagctgattt gtttgaccca
    14881 tcttttccca aagatgacct tttcttaaca acgaagagga aattaaagac gaaggtactt
    14941 gaatatggtg cgaatgcagt aatcaactgt cattttgagt atcagcaaaa aaaacgcgac
    15001 ggaaagtatt ttctggaact ttttgcttat ggaacagtga tccaatttat ccaaacaacg
    15061 attggttaaa tgaaacagac aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct
    15121 tttttctatc aaagttagtg actacttcat cgtatactct gataagaatg atttctagta
    15181 aaatatactt ggtttgaaaa actttgtcaa cgtgatttat tcaaaaagta atggaatcaa
    15241 taatattttt tagcaggttc tactactaaa taacgataag gttcctcacc ttccgaatgt
    15301 gtttttacat gttcatcttt gcttaatgca gcatggattt gtttacgttc aaatgcaggc
    15361 attggttcta agaaaacagg ctgccctgtc cgttttactt tttcagccgt gcgatcagct
    15421 aaacgctgaa tgatctcttg gcgcttttca cggtaattgc ctacattgac tactacagac
    15481 agcttatttc ttgctaaacg atggatgaag acttgtgcca gatattgcaa tgcattcagt
    15541 gtttttccat gttttccaat cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc
    15601 atattatttg attctctttc cattttgaca agagctggag catcaagctg ttttgaaatt
    15661 tcagtaagat aaactgccaa ttccgttaaa gctgcttcat cacctagctc acttacttct
    15721 tctgtttttt cttcaagaat cgtttcgttg actacctcga tctcagctgt tggttcttct
    15781 tccaaaattt cttctacagt ctgttctact gtttcagaaa ctttttcgct gatacttggc
    15841 tggatcgaaa gccgtgcatt ctttcgaccc attcctagga aacctttttt tccttcttcc
    15901 agtatttcga tttcgacttg gtctttggcc aaacccaaat cagccagacc tagttggaca
    15961 gcttcgtcta ttgttgcagc ttcataaatc ggcattagca ttacctcccc tattatttcc
    16021 gttttttctt aggattcatt gctttcttaa gagcacgttc tctttcgcgg attctccgtg
    16081 cttcttcttc acgctctttt cgaattttga aagggttgtt gatcagcagc gtttgtacaa
    16141 cttggaaggc attggaaact acccagtata atgaaagacc gctggctaaa ttgacaccca
    16201 tcactaaaat cattacaggc atcacgaaat tcatgatctt cattgatgca ttcgattcaa
    16261 tttgactcat gctagataaa tacgtacttg caaaagtaaa gattgctgct aaaatcggca
    16321 aaataaatgt tggatccgcg ttccctaagt tcaaccataa gaaatgccct tgtttcaact
    16381 ctggtacacg tgaaatagat tgccataacg ccatcaagat tggcatttgt actaataaag
    16441 gaagacaacc agcatatgga ttgacgccat tttctgcata taaacgctgc tgctcttctc
    16501 ttaacttact ttgtgtttcc ggatctcttg aactatattg ttgctgcagt gcttttaatt
    16561 tcggttgtaa ttcttgcgtt ttacgcatgc tctttgtttg gaaatgcatc aacggcaata
    16621 ggatcacgcg gatgatcagt gtaaataaaa tgatccctat ccctgcatta ccaaatgaca
    16681 acgccttgat cgcctcagca aagtaataaa caatataacg atcccaaatt cctgtactct
    16741 gtgcgctgac ttccccagtt ccgcaaccgg ataagacgat gaccaaacta acgataccgg
    16801 ccatcaaaag taaacgtttg tatttcttca cttactcttt tcctctctgt cgattatttt
    16861 tgcaagtttt aagacgtgga ctaaattgga gcgaacctca tcataagtca accccttcac
    16921 acttggtcta gcaatcacaa taaaatccaa ctccggatca atttcttctt ttatagaata
    16981 aactgctgct cggatttttc gtttgacttc attgcggttt accgcgttgc cgaccttttt
    17041 tcctacagat aaaccaactc gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac
    17101 gaatttgcgg ttagcaaaag aatctcccgt atggaacacg gcttgaaact ctcgttcttt
    17161 ttttactcga tacgcttttc tcattagttt tctttccttt tctatctctc acttacgctc
    17221 ataatcatac cataattacg attgtccttt catcatttcc ctaaaaaaga gtataaaaaa
    17281 aaccactgaa accccagtgg cctaagctga aagaactttt cttcctttgc gacgacggct
    17341 agctaacaca cgacgtccat ttttagtact catacgttta cggaatccgt gaactttttg
    17401 acgtttacgt ttatttggtt ggtatgttct tttcatatat tccacctcca aaatgttctt
    17461 atctatttta taaacacaga catactttga tagtataacg acaaagtagt gattttgtca
    17521 atataaaata gaagatcgat tgttaaaact tatccacaag attatttttt tctatgggaa
    17581 tcctatgtgg aaaaagttat gcacagggat tatccacaga taataaaagg ctggttttac
    17641 acagaaaaca cacgtgtaaa aaaagtaatc cacaatcagg cgatttcctg tgtattccgt
    17701 ctcctaacta ttggtatatc tgtatttttt atccacagac gcaagaggaa atctattggt
    17761 ttttacgagt tttccacctg tgcattagtg gtgtggaaaa ctttttttga caactgtgtt
    17821 tttctctttt ttctttttgt cgattgtgtg gataattgta attaaaaaat ttttttcttc
    17881 ggtttctgtg gaaaactatt gaaaaaaatg ctaaattagt actagttatt cactttttaa
    17941 tgaaggagga ggccttatat ggtatccctc gatgctttat ggaatgaatt aaaagcaaca
    18001 taccaaaagg atttatcgcc tgctagctat aatacatgga tcgaaacagc gaaccctcga
    18061 acccttgatc aaaaccagct cgttgttgag gttcctagca agattcataa agaatattgg
    18121 gaaaaaaacc tggcgacaaa aatcgttgaa gtcggttata tgttatcagg aaatgagatt
    18181 atccctcgct ttattactgg cgaagaagca gagcaagaag aagttataga agaaaaaaat
    18241 ccaaaagttg tggcacctag tccgctaaaa aaagccatgc tgaaccctaa atataccttt
    18301 gatacgtttg tcattggtaa aggaaaccag atggctcatg cagcagcgct cgttgtagca
    18361 gaagatccag gttctattta taacccgctc ttcttttatg gaggcgtggg acttggtaag
    18421 actcacttga tgcatgcaat tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta
    18481 aaatatgtaa gcagtgaaac ttttgctaat gatttcatca attcgatcca aaacaaaaca
    18541 gcagaagagt tccgtcaaga atatcgaaat gttgatttac tgctagtgga tgacattcaa
    18601 ttttttgcag aaaaagaagc tacgcaagaa gagtttttcc atactttcaa tgccttgtac
    18661 aatgaaggga aacaaatcgt actaaccagt gatcggttgc caaacgagat tccgaaactg
    18721 caagaacgcc ttgtttctcg ctttgcatgg gggctgtctg ttgatattac accgccggat
    18781 cttgaaacaa gaacagcgat tttacgtaaa aaagcggcgg ctgaacgtct agagatccca
    18841 gatgacacac taagctacat cgcaggacaa attgattcta acattcgtga attagaaggt
    18901 gcgcttgttc gagtgcaagc ttttgctgcg atgaatagtg aagatatttc gactagtcta
    18961 gctgcagacg cactgaaaac gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg
    19021 cagatccaag aagaagtggc aaagtattat cacatccaat tgaaagatct caaaggaaag
    19081 aaacgcgtca aatctattgt tgttcctaga caaatcgcta tgtatttggc acgtgagctc
    19141 acagataatt ctttaccgaa aatcggagct gaattcggtg gcaaggatca tacaacggtt
    19201 atccatgctc atgaaaaaat ccagcagctg atggattcta gtgtcagtat gcaaaatgaa
    19261 gtatcagaaa tcaaaaactt tttactaaat taatttgtcg attctatcaa ctgaaaatag
    19321 catgtggata aaagaaaaaa acgttcaaaa gttatacaca ctttttccac agctgttttt
    19381 tcgtttctct cttacgtttt ttgagttttc cacagaatca acatgcccta ttacttttat
    19441 tattcttttt aactaataat aaataaacaa acattcaaag gagatacatc atgaaagtta
    19501 ctttaaaccg agctagcttt atgcaggaat tgcaaactgt tcaacgagct atttcaagca
    19561 aaaccacgat ccctattttg acaggtgtaa aaatcacact gacacaagaa ggtttgactt
    19621 tgacggggag caacgctgat atatcaattg aaactttttt gtctgttgaa aacgaaaaag
    19681 caaatatgca aatcgaatct actggttcca ttgttttaca agcacgtttc tttagcgaaa
    19741 tcattcggag acttcctgaa gaaacattta ctttagaagt tttagaaaat aaacaagtag
    19801 cgatcacttc tggaaaagcg aattttatcg taaatggatt agatgcagat aactatcctc
    19861 atcttcctgt tgtcgaaagc cataaccaga tgaaattacc tgtacacgta ttgactaaac
    19921 taatcaacga aacagttttt gctgtctcac aacatgagag tcgtccaatc ttgacaggtg
    19981 tgcatttcat tttatctgat aattctttat tagctgtagc tactgattct caccgtctaa
    20041 gtcaacgcgt gattccagta gaacaagcgg ctgatcattt tgatattgtt attcctggaa
    20101 aaagtttgat cgaattatct cgctcattaa caaatgaaga agaaatcgtc gaaatcagca
    20161 ttatggaaaa ccaagtgttg ttcaaaacag aaacgatgta tttctattcc cgtttgctag
    20221 aaggaaacta tccagatacc aatcgtttga ttccttcaag ttttaataca gaggttgaat
    20281 tttctgttcc tagcttttta gcggcgatcg aacgggcttc tttactttct catgaaggcc
    20341 gtaacaacat cgttcgttta tctattcgtc cagatgcggt tgttttatac ggaaattcgc
    20401 cagaaatcgg gaaagtagaa gaaagcttga gttatacagc aagcagtggc gatccattag
    20461 atatttcttt caatccggat tatatgaaag cagcgttgcg tgcatttgga gatatgagca
    20521 tcaaagtgaa atttatctct gctattcgtc catttacatt ggaaccaacg gaagatggcg
    20581 ttcaatttat tcagctgatt actcctgtac gtacaaatta acaatgattt tcaatgttct
    20641 tgaaaattta attgaaaaga aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc
    20701 tcttttaagg ctttctgttt atttttgttt aa
//
LOCUS       DAGCWU010000034        20458 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.034, whole genome shotgun sequence.
ACCESSION   DAGCWU010000034 DAGCWU010000000
VERSION     DAGCWU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.034"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            854..1198
                     /locus_tag="MBZ71_000995"
     CDS             854..1198
                     /locus_tag="MBZ71_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBT4428966.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1203..1484
                     /locus_tag="MBZ71_000996"
     CDS             1203..1484
                     /locus_tag="MBZ71_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428967.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNIFGYSIEEINYYIDFAEAFIPMVR"
     gene            1587..1901
                     /locus_tag="MBZ71_000997"
     CDS             1587..1901
                     /locus_tag="MBZ71_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBT4428968.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1879..3573
                     /locus_tag="MBZ71_000998"
     CDS             1879..3573
                     /locus_tag="MBZ71_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBT4428969.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPGLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENAGTGFTPLVF"
     gene            3593..4771
                     /locus_tag="MBZ71_000999"
     CDS             3593..4771
                     /locus_tag="MBZ71_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBT4428970.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRAQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYKLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4734..5420
                     /locus_tag="MBZ71_001000"
     CDS             4734..5420
                     /locus_tag="MBZ71_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBT4428971.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKREEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5420..6580
                     /locus_tag="MBZ71_001001"
     CDS             5420..6580
                     /locus_tag="MBZ71_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBT4428972.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTRA"
     gene            6590..7465
                     /locus_tag="MBZ71_001002"
     CDS             6590..7465
                     /locus_tag="MBZ71_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428973.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7462..7773
                     /locus_tag="MBZ71_001003"
     CDS             7462..7773
                     /locus_tag="MBZ71_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428974.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7763..8116
                     /locus_tag="MBZ71_001004"
     CDS             7763..8116
                     /locus_tag="MBZ71_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBT4428975.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8106..8507
                     /locus_tag="MBZ71_001005"
     CDS             8106..8507
                     /locus_tag="MBZ71_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428976.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8500..8904
                     /locus_tag="MBZ71_001006"
     CDS             8500..8904
                     /locus_tag="MBZ71_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428977.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8916..9524
                     /locus_tag="MBZ71_001007"
     CDS             8916..9524
                     /locus_tag="MBZ71_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4428978.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9544..9906
                     /locus_tag="MBZ71_001008"
     CDS             9544..9906
                     /locus_tag="MBZ71_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428979.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9909..10091
                     /locus_tag="MBZ71_001009"
     CDS             9909..10091
                     /locus_tag="MBZ71_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428980.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            10108..13527
                     /locus_tag="MBZ71_001010"
     CDS             10108..13527
                     /locus_tag="MBZ71_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBT4428981.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFIDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAVATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            13578..14315
                     /locus_tag="MBZ71_001011"
     CDS             13578..14315
                     /locus_tag="MBZ71_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4428982.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFTCTGLSKIQIYRNGLR"
     gene            14325..16616
                     /locus_tag="MBZ71_001012"
     CDS             14325..16616
                     /locus_tag="MBZ71_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4428983.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNVGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16640..18883
                     /locus_tag="MBZ71_001013"
     CDS             16640..18883
                     /locus_tag="MBZ71_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4428984.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGNYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAETEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGV
                     DYSPADGKKILIRYRFVDNGTGKIVLDLPINSNSITQEWKEFSITGTTPTFGPQAYHP
                     WIQFRAQDGILGEIEMSYDIKIEEGPTATPFQPNLLAEPYNMCREYPNENIVNPTVKF
                     PIKTSNNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQLQHQATLLP
                     VEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEKGNTRTPNIS
                     EYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQ
                     LTASINEVDTTAKDARTKANANATAIDELDNKIDERINDAATTAFYGAQGEVSNKTKV
                     AEYGVGTGYTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLY
                     KDGTSVDNLVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTL
                     VLTRLSD"
     gene            18961..19203
                     /locus_tag="MBZ71_001014"
     CDS             18961..19203
                     /locus_tag="MBZ71_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBT4428985.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQVYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            19219..19416
                     /locus_tag="MBZ71_001015"
     CDS             19219..19416
                     /locus_tag="MBZ71_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBT4428986.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            19427..20446
                     /locus_tag="MBZ71_001016"
     CDS             19427..20446
                     /locus_tag="MBZ71_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBT4428987.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         7224 a         3041 c         4314 g         5879 t
ORIGIN      
        1 tattaattta tcgtaataac ttttgagtaa atagaacaaa aaacctgcac tagtttccgc
       61 tagtacaggc agtgactata tcggttgata atctagcata tattaaaaat aattgcaaga
      121 aaagaaattt atagtggttt ataccaaatt atcaaagcaa tgttattttg ttgctgtcta
      181 ttgtttttta attattcaca cgataactaa aggtgggtga agagaaatga ttccattaat
      241 aatttcaatt tttgcgctct gtcttaatgt ctatatgatt ggatttaaaa atgggcaaaa
      301 taaaaaatag tagcagccaa gaataatttt atagtgtcac tgtggcggaa agggtagacg
      361 ctaagcatgt gtgctaggtc aatgcttcgg caaccatgca aggttcgatt ccttgccagt
      421 gactttggca accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg
      481 ttttgtgcat cgtgtaggtt gctattacat aactggttag gttagattga gatttgggat
      541 tcggtacaaa tgaatcgtca aatgtctcaa gcacaggatc ggaaacgtcc ctgcctgtgc
      601 attacatatt agatcactcg ttgagtggtc tttttatttt gcaaaaagga ggtaacaaca
      661 atgtatagac cacaatactt agaacagaag catgaagtaa tcactgtgca aaacggtaac
      721 ggtgagatag tacgaaagta tagaagacca ataaagagca atacatataa acgaaaggaa
      781 agcaatgaag ttattccatt gtatggcaaa agaatagcta agtattaaat aagattgcga
      841 aaggagatgg gacatgaccg aggaattcta tagatggtta ttacagttga taagagaaga
      901 tcgtttggtt aagttctatc agtctcctaa atggcgcagg cttagagaga aagcgatgaa
      961 acgagatcac tatgaatgcc aagagtgtag aagactaggt aagtatcata gagtagagaa
     1021 cgttcatcat ataaaggaag tcaaggatag acctgactta gctttagatt tagataatct
     1081 tatttgttta tgtgttgaac atcataatga agttcatggc agatatctta cagcattaga
     1141 taaacaagag aagaagatag aaagctttgc taacttcgat gcaagtgaaa ggtggtaagt
     1201 gcatgatcat caatgacaat ggcagagagt atgacacaga aaagattgaa gagtattcat
     1261 cttatactca aggattaatt aaacgtttga tatacgttcg ctatgtaggt attagggatc
     1321 tgttatcaga taactgttgc agtaaataca aagtgaatca agtaagagaa gcgttgaata
     1381 aagataataa cgttgaaaga ataaaaaata tttttggata tagcattgaa gagattaatt
     1441 attacattga cttcgctgaa gctttcattc cgatggtgag ataacccccc cttaaaataa
     1501 atcgcaaatt ttttgggggt gatgaaacgg agggggctgt caggaaaaga gattttttcg
     1561 aactttatca tgaaaggagg gctaaaatgt ttaaaaacga attgtctcaa aatcgctaca
     1621 gagaaaaatt acgccgctct ttaataagcc aattggaaag tcagaaaaca aatattgagc
     1681 cattcttaga taatgttgat cgttatatca gtttatggga aacggcgata tcactggaag
     1741 aagatatatc cgagaacggc attagactgg agaatggtaa aaagaatgaa tcagtagcgt
     1801 tgcttgtttc tgtcaacaaa caaatgggat tgatgttgga taaacttgcc attactcctg
     1861 aattggtagg tgaagcaaat gaatcaattc ctgagttata agcatattga aaattggttc
     1921 aaagctatag aagaaggcac tatcaaggta tgcaaagagc aattattgct aaaaaattat
     1981 ctagaagaaa gagtctttac tagagaagat atttacttcg ataagcagat ggtagaggat
     2041 tcaatcaata taccagcaca atactttcca ttcgaattaa ttccgtggga aaaatttcta
     2101 caatgtttta tttatggtgt tcgatggaaa aaagataaaa cgctagtgtt caatagatat
     2161 ctttcattaa tgggacgtgg taatggtaaa actggttttg cttcttggaa caattttttc
     2221 ttgctgactg caaaacacgg tattaaaaat tatgatattg atatctatgc caataacgaa
     2281 agccaagcaa agactagttt tgatgatgta tttaaagtaa ttaaagatca tcctggttta
     2341 gataaaaaag tatttaaagc tacgaaggaa gttattcaaa atatcgctac aaacagcaaa
     2401 cttcgttata acacggcaaa cgctagaaca aaagatggta aacgaccagg agcaaaccgc
     2461 tttgatgaaa ttcacgaaaa tgaagattat tcaatgataa atgtagctac ttctggtggt
     2521 ggtaaaattc gagattatag agaattttat gatacaacta atggtcatgt tcgtggtggt
     2581 ccgcttgatg acattataga agaatcaaaa atgattcttt ctggagaact tggaattgac
     2641 aaggatggag cagaattttc tagtttgttt ccatttattt gtcgcttgga taatgataat
     2701 gaagttgatg atcccgatat gtgggaaaaa gcttgtccaa ctattaatta caatgcagat
     2761 ttaaaacgga aaatgtttca agaatactct caaatgcaac gtaatgctgg tttaagactt
     2821 acgttcatga cgaaacgaat gaatagacca atggaagata cgcgatttgc tgtagcttca
     2881 tatgatgatg ttcttcatac gaaagaaaaa gaatttcctg aaaaaatgga tgaagtgata
     2941 ggaacagttg attttgctga tagacgagat tttgccagcg ttggtttgtt aggaaaatat
     3001 gataaagatg tgtattttac acaacatact tttatccacg aatcagctct tcgattacag
     3061 aacataaaac gagaggttat agatatttct atagatcaag gaaaatcaca gatcgttcat
     3121 gggaaaaata tagaagctga ttatattgta ggttggtttc ttgaaatgag taataaatat
     3181 tacattaaaa aaatcgctat ggatatgtac cgtgcaaaaa tattgaagcc cgctttagaa
     3241 gaagcaggtt ttactgtaga aattgttcga agcggatctg ttacacatgg tatgttaaaa
     3301 gacctagttg atgacctttt tattaatcaa cgtttatttt ttggtgacga tgcgattatg
     3361 cgttggtatt gcatgaatgt atatgaagag catatttcta atggaaatat acgctatgaa
     3421 aaaatagaac ctgaaactag aaaaacggat ggcttttttt cattccttca tggtttgaat
     3481 tttttagatg atatttatga ttctgctcct gtaacagtca caaatagttc agtagaaaat
     3541 gcaggaactg gatttactcc tctagtattc taacttgaaa ggaggtgaga aagtggggat
     3601 ttttcaaaag gcggtaggat acttcacaaa aaaagcaacg gttcctttag aagaatactt
     3661 ttgtaaattg caagttgatt ttgtgtatcg aaaatttgca attgaaactt gtattgattt
     3721 gattgcaaat gcgatgagca aagcggaatt caagtcatat gaagatggga aaaataaaaa
     3781 gaatgatctt tactataggc tgaatgtagc tcctaataag aaaaataatg caacagaatt
     3841 tagaaaaaaa ctgatcagga gattaatatt ctacaatgaa gtattgatcg tttctccgtc
     3901 taataattct agcgaaatat ttattgcgga tagttgggat gtcacagagt atgcattaaa
     3961 agatgatgtg ttttctcaag tgcaaattaa caacatagtc cttgatagag aatttctaga
     4021 aagtgatgtt atctatataa aatacgcaga tcaacaaatt aggcaactag tcgatgcgta
     4081 ttatcaagcg tatgggaaac tcatttctag tgccatgaat gtttacaagc gttctaacgc
     4141 tcgtagatac gtactgaaag ggaatttatt ccgagcgcaa gacaatacaa cacaagatca
     4201 aatcaataaa atgatgacat cacaatttaa ggcttttatg gaagctgata atgcaggtgc
     4261 ggtatttcaa ttacaaaatg agtacaaatt agaagatttc agcggaaact ttcaaagcaa
     4321 ttcaagagat ataaaaaact taatagacga catctttgag atgacagcag cagcgtttca
     4381 cgttccgaaa aacctactaa agggagacat gagtggatta tcggatcaag tggacgcttt
     4441 tttaatgttt gaaatcatac cgattgctga acttattcag gatgcgttta acgctagtct
     4501 ctatgaagta gaagaatact tgtcagggaa ttttgtacgt gtggatacaa ctatgatcaa
     4561 gattactagc ttcaaagatt tggttgacgc tattgatgta ggcattagaa atggggtatt
     4621 tacaatcaac gaaggaagag aacgcgttgg aaatgatcgc tctgataagg cgatggcaga
     4681 tgaaatattt ataactaaaa acaatcaaca agtatcgaaa ggaggtgagg cgaatgacga
     4741 caatgaaaac atttctagca gtaaagaatg aaggcgcagt accgcaaatt tttattcagg
     4801 gatttattgg ttctagttgg ttctttgaag ggaatactga caagggaatc aaaaatattt
     4861 tggatagtct aggtgatcaa gaagagattg aagtagtaat taattcaaac ggtggagacg
     4921 tatttcaagg gattgctatt gggaacttac ttaagtcaaa taaagcaaaa gttaacgttg
     4981 tgattaacgg cttagccgct agtgctgctt caattatcgc aatggctggc gatactataa
     5041 aaatctacaa caatgcacaa ttgatgattc accgcgcttc cacatacgga gaaggaaatg
     5101 tcgatgactt ccgtacgatt gctgaccaac tggaatcaat tgataaatcg gtaaaggctt
     5161 catataaaac acgattcaat ggcacagatg aagcattgca agaacttctt gaaaaagaat
     5221 cgtttatgga tgcagaaaca gctttgagtt atggattggt cgatgaaatt atcgatgcag
     5281 aaaatagctc aggtactgaa gctaaaaaag aacaaagcgt tgaagaaatt ttgaatgacg
     5341 ttaaagaaaa aagagaagaa aaaattgctg catttacagc agcattaaat aaaacatttg
     5401 gacaaggaga tgtaaaataa tgacagttaa aaatttaaaa ggtgtaacag ctgcaagcga
     5461 ccaattgatg aaagctttta aagatggtaa cgaagaatct tttagcgcag ctatggtaag
     5521 cttatctaag gaaattcagg ataaaatttt agaagaagca acagcaaaaa atcaagatca
     5581 attagtatta atgaaccgtg gtcagcgtgt attaactaca caagaaacaa aattctataa
     5641 cgaagtagtg aaaaacgaag gttttgcagg ggttgaagaa ttagtgccag ctactgtatt
     5701 tgaacgtgta tttgaagatt tagaacaatc tcatccacta ttgcaaaaaa ttacttttgt
     5761 taacacaact ggtgtaacag aatggattgt gtcacgtgga gtcaatccag catggtgggg
     5821 taaactttgc gaagctgtta aaaaagtttt agataatggc tttgatgtaa ttaacatgaa
     5881 gcagttcaag ctatcaggtt atattcctgt atgtaaggca atgcttgatt taggtccagt
     5941 atggttagat cgttatgtcc gtactgtttt agtagaatca ttgagaattg cattagaaca
     6001 agcaattgtt gatggtactg gtaaagatat gccagtcgga atgatgcgtg acatgagcaa
     6061 acaaactagc ggagaatatg ctgaaaaaaa agcagaacct attacagctt tagatgctgt
     6121 aactatgggc ggtttgatgg cgcgactatc aaaattcaat atcgaaggtg tgaatgatcc
     6181 gatttatcgt aatgtgaatc cttctgatgt ggtcctaatt gtgaatccaa ctgattactg
     6241 gtctaaagta ttcccagcta agactgtact aactgctaat ggagaatacg tacaagtatt
     6301 gccagtacca gtttcagatt tgcagtcaac ggctgtgcca gaaggaaaag cagttattgg
     6361 ggtagcctca gattacttca tgggtgtagg atctacacta aaaattgaag cttcagatga
     6421 ataccatttt gttgaagacg aacgcattta tctagctaaa caatatgcaa acggtcaacc
     6481 taaacgtaac gatagtttca ttgtattaga tattagcgct ttgggaacta ctactacaac
     6541 tacaaaacca acaaccacaa caactacaac acgagcgtag gtgatcagaa tgaagtatat
     6601 tctttgtcag ccggcaatca atcggtttaa atgggagctt gaagtttgtt taactaatct
     6661 gaagaaacta ggaatcaaag atatcgtatt gcttttcagc agacacgatg atcagattcc
     6721 tatttttttt gagaaggaat atggtgttga agttcatgtg tacgatgatc tgcgggacga
     6781 caaagagtat attccttcga ttaaaccata tttatggtgg aaatatttag aagaagatca
     6841 ttcgcgtgag gacgaccgat atttctatat cgattcggat gtcattttca ataaaagaat
     6901 taatttgcgc aaattgcctt ctaaagatga tgtttggtat tgtagcgact gctgtagtta
     6961 tctaagtctt gattatatta gaagctgtga aaacggagaa aatattctaa aagatatggc
     7021 aaacattgta aatgttacag tagaatcttt ggaaactata aacactaatt caggaggcgc
     7081 acagtgggtt attaaccgtc ctaaagcgaa ttattggaaa aaggtttatc tggattctaa
     7141 tcggctatat cgctacctta gagggcaaaa aacaaatata caaatctgga cagccgagat
     7201 gtgggcacag ctttggaaca tgatgtattt caatattggt cctaaagttc acgaggaatt
     7261 agacttttgt tttgctactg atccaataga aaaagttaaa gaagtaaaaa tcttacacaa
     7321 tgctggagta acaacaaacg atgaagattt atttttcaaa gggagatacg tgacttctac
     7381 gccttttgat gaagatttat catttgtaaa caagaaaaaa tgctcttacg catatgttaa
     7441 agcaattaag gcggtggtta gatgacgcct gaacaagtga ctgaagaatt gctaatagct
     7501 gtgaaggata atatttacgt tacctggaac gaagaagatg agtcaattaa aaagatgata
     7561 gctaaaaatg ctgtttatct tcaaagtaaa gtgagtacaa cactttcttt ttctcctgaa
     7621 agcttagaat acggattgct aatcgaaaga tgtagatacg actggaatcg tgctttagat
     7681 gagtttgaac aaaatttcgc tagtgagtta ttaggtttca ttcaacatta tgcgctacaa
     7741 gaatatattg caggtgatgt gaatggcgaa taatcgtaga ctcgaagaaa cattcaacga
     7801 tggttggtta aagattttga cgcaaactac caaaagaaat gaactaggaa aaaagattgg
     7861 tgtagaagat acagaaatca cttctttaaa atttagaaat ctttccatga gagatagtga
     7921 tataacagct atggatgcga tgggatcgaa attaactaag aaagtaaaga ctccatttca
     7981 tccaatcgcc aagaaattta ataaagatca atattttatc gtaatcgata gtatgcgtta
     8041 caacgttatc tatgccgatt acgataattt ttatatctat ttttatcttg aaagtgtggg
     8101 tgaatatggt gattgataat tctaaagaaa aagaacgttt aaataagcaa atttctgcta
     8161 tcaaaacttc cttagaagag cattttggcc tcaaactctt tcaagactcc gtaggcgagg
     8221 atgagctacc tgatgatttt aattacttca ttctcgaaac aggagaaata gaaatgatca
     8281 ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac tttctattca gaaaatagag
     8341 aagatttaac aggagactca ctagatatta tttcattgat tcaaaatcgt tcgattcgtt
     8401 ttcagagaat ggatcccaat catttaaaac tagagaacca agatcgctat atcgatcaat
     8461 tggtatttac gtttagacga ttattgaaga gtgattgtca tggctaaaaa tagttgggag
     8521 ctaaaaataa atggacatga tgaacttctt gtgcggatgg aacgctattc aagcgagagc
     8581 gaacgactga ttaacgaagc attgaaatca aaaggttcgg ctattgcagt ggataggatt
     8641 acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca cgcaaaaaat
     8701 agtcgtccac ttaagactca atacattaat ttgggtttca tcattagacc tacaagaaaa
     8761 tttgagtatt taaaatatcc tgatttgggg ataggtactt ctaaaagaaa tcagccggac
     8821 gaatttatga gaagaggatt aggtcttgca cttgatccaa ttacagaact tctgattcgt
     8881 caattcgata aattaaataa atagggggaa caacaatggc taaaacaaaa actgtagtaa
     8941 caacgttcga taacgtaagt atcaaacgaa ttgcttttaa ttttaagaac gcaggaaatg
     9001 caatcgcaac gaattgtaac ggacaattag atggcgaaac agaaatgcaa acggtggtta
     9061 aaaaatgtgg agcgacagaa gtaaaatcaa aatctaaacc aatcaatatg acggtaacaa
     9121 ttactgcaca tgtaccgatg gaagtttatc gacgtttcaa tgggttgaaa caagatggac
     9181 gtattaaacc aggcatttac tcttacggtc ctgattccgt aggcgaagat ttctcacttg
     9241 ctgcagagat cgtggatgac ttcgaagaaa atagaaagtt agttggtatg ttagcatgca
     9301 cttcgaatac aggattaaca ttctctattg aaaatggtgc ggatgaagta gctgcgttag
     9361 aactagaaac aaaagttatg caagatgaat ttggtaaatt ttatcatgaa gcaattgttg
     9421 cagaacttga agaagactta acagatcaat ggatgacgaa tctatctgct gatgtgatta
     9481 aaaaaacttc agttgtgaca actacggcca ctcaatcaca gtaaaaaaaa cggaggtagc
     9541 gaaatgaacg aagattactc aaaaattgaa ctaaacgatg gaacaatttt gaatttagaa
     9601 cctaaactga atatcaagaa attattgatg atcaatagag attttaacac agacgagttt
     9661 gcaaaaatga ctgtgggaaa aggatccatg gatatttctg ttattcaagg tgcaaaggct
     9721 gtgtatattg cttaccgcca agcgaacatg actgattata tttcattcga tgaatttatc
     9781 gataaatggg attttgatat ggctactgcc agctatattt atcaattgat gatgttcaaa
     9841 caagcacgcg atgcttatca aaaagaattt gaaaaagcaa ataaggaaaa aaagcttcaa
     9901 aagtaaaaat gccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg
     9961 acgttttttc tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc
    10021 aaatggctta caacaagagt gcttatgaag gatggaaaaa ctatgcaata aaccaatctc
    10081 agaaaaacta aagaaaggag gtaaaaaatg gctaaaaaga gaacagaagc agaagtaact
    10141 ttcatagcta acgatgacgg attgaaatct acgttaaaag aaatcagtgc tgaattaact
    10201 aaaaatagag cagaattaaa actagaacaa gctcaattac aacagactgg ttctgaatca
    10261 gacaagttag gaagtaaatt atcttcttta gaaaagcagt atgaattaca aagccaaaaa
    10321 gttgaagtaa ctagccaacg tttagccaat gccaaaaaat attatggaga aaattccacc
    10381 gaagttcaga aacttgagag agaactgatt aaccaacaaa cagcgcaaca acgtttgtca
    10441 aacgaaattg ataaaacgag taatgcacta gctcaagcaa aaggcgaaat acagacgtac
    10501 gagtctacaa tgcaacagtt ggatagtgaa caaaaaaatg ttcaagctag tgcttctctg
    10561 attgaatccg aatacaaaaa atggcaagta actgctggtc aatcagcttc tgaatccgag
    10621 aaattagcga aagcccaaga atatgtttct caacaatctg aaaatgcaga gaaaacgata
    10681 gatatcctga gacgacagtt agaagctaca cagtctgagt ttggcgctac atccacagaa
    10741 gcaatgcaga tggaggcgaa gcttaatgat gctgaacgtg aatttgaaga gttaggacaa
    10801 gctgctaaaa atgtagatac aactaacttg gacgatatcg gaagcaaaat agatatgaat
    10861 aatttaatgg aagcttctga cgttttaagc gacattggcg ataagcttac agaattaggg
    10921 aaacaagcag tggactcagc taacagtgta ggtagttccc agagtaagat acaagctaat
    10981 tttggtttga ctaaacaaga ggctgaagaa ttaacgaatg tagccagaga catttattat
    11041 aaaggttttg gagaatcgtt agatcagtcc acagatgcat tgattttggt aaagcgtaat
    11101 ttaggcgatt taaataatca agatttacaa aatatcacgg aacaagctat ggtcctagaa
    11161 aacaccatgg gcgctgatat ggatgaaacg ttacgtggtg taaatggctt aatggtcaat
    11221 ttcggcttga gtgctcaaga tgcaatggat ttaatggttt cgggtactca aaacggttta
    11281 gataaaacgc acgaattagg cgacaatatg gcagaatata gccaattatg gagtcaaatg
    11341 ggatattcag ctgatgaaac gttcggaatg cttcaaaatg gtttagatgc gggtgcttat
    11401 aaccttgata aagtcaatga cttagttaag gaaatgggaa tatcgttaac agatggtcga
    11461 tttgagcaaa acatggatat gtttagtgaa agtactagaa aagcttttga agagtggaaa
    11521 aatggcggag gaacacaaaa agacgttatt aattccatga ttcaagattt tagcaatatg
    11581 gatggtcaat acgaccaatt aaataaagct tctacaattt ggtctgcact tggcgaagat
    11641 aacgcgatga aagttgtcca atctttgact gatgttaacc atacatttga tgatgttagt
    11701 ggatctgcac aaaaaatgaa tgaagattct actactccgt tacaagaatt aaacggaaaa
    11761 atagctgaat taaaggattc attagctcct ataggcaaca caatcataga tgcactcgaa
    11821 ccagtaattg attttctagg aaagatggct gatgcgttta ataatcttcc acaaccagta
    11881 caggattatg ccgtagcgat tggcggattg actgctgcat ttactttatt aatgccaata
    11941 atagttggct tcatggctct aggtggtcct actacattaa taataggagc agttattact
    12001 gctattgctg gagttatagc aattataaaa aactggggcg caattactga ctggtttaag
    12061 ggaatatgga gtaaattcac tgattggttg ggtggtactt gggaaagcat aaaagaaggt
    12121 gcctcatcag tttgggatgg agtcaaagaa acctggtctg gatttgtaga ttgggttcaa
    12181 gaaatttggc aaggagtttc tgattggttt ggagagttat ggagcggatt agttgaagga
    12241 gcttccaaca tctggcaagg agtccaagag acttggcaaa cattcattga ttgggtttca
    12301 aatatttgga acggagtcaa agaagtatgg tcgattattt gggcagacat tgtaggaatt
    12361 gttcaaatac catggacatt aataacgtca ttgattcaag ctggtattaa tattatcgtg
    12421 ggtatttttg atgtagctgg acagttatta ggcgcagctt ggcaagctgt ttggacacct
    12481 atttctgatt tccttaaaaa tacttgggat actatgacac aatggataag catcgcttgg
    12541 aatggaattg taactacatt ccatactata tttgatccag tagtggcatg gtggaatggt
    12601 atatggacat ctattagtac tacggcttca aatatttgga actcaattag tgcaacagct
    12661 tctagtattt ggaatagtat caagaataca atcactagct tggtacaagc agttgctaca
    12721 gtaattcaaa atatttggtc aactgtatct agttggttag gtggaatttg gaattcaatc
    12781 agctctacag catcaaatat ctggagtagc gtgactagta gtataagcaa tgctataaac
    12841 gcagctaaaa gtgccattca aagtgtttgg aatagtatat cttcgtggat cagcggaatt
    12901 tggaacggta tcaaaaacac tgctttgaat ctttggaatg gaattacaag cactattagc
    12961 tctaaagtaa acgatggaaa aaatgcaatt tcaagcggtt ggtctaatct aacaggtatt
    13021 gtttccgaca tattcaataa tgttaaaagc acgatagcta atatatggga aggtatcaaa
    13081 aagactgtta gcgctccgat tgattggatt agagataaaa tcagtagtgt ctttgataat
    13141 ttgaatattt ctataccaca tattccgtta ccacatttta aattgagcgg agaattcaat
    13201 ccattgaagg ggaaaatccc aacattgggt gttgattggt atgcgaaagg tagtgtgttt
    13261 aattctccaa atattatcgg tgtcggtgaa gcaggacctg aagcagtttt acctttgaaa
    13321 agatctgtgc tgcaagaaat tggggatcgt atcttgagta gcacctcagt ttcatctagg
    13381 gcacaaacga ttcaacctgt gaacaattac gaattcaatt tcacaattga tggtaacgca
    13441 gatgaagtca ctatgaagca aacaactcaa caaatcattg atagcattac aaaagttcaa
    13501 aatgataatg cttcggcatg gcgttaaaca ggagagtatt tctcctgttt ttttagtttt
    13561 aaaaaggatg tgaaaaaatg actgattgta tacattctat aatcgatgga tttcctgatt
    13621 atttgcataa attggcttta gcggaaagac caaccatacc ttctccaaaa agacagagag
    13681 ttgaaacttc tgttttagga cggctaggtg gcttagtaca agattactcg tttgaagaca
    13741 tgtcgtttac attgcactat aactatttag aagatgtgga agaccatcaa gcattcaagc
    13801 aatcgtttta tatcatgcgt cattggttaa actatgcaaa gaaattagaa ttctctgatg
    13861 atcccaacgt ctattatgtt atccagacta tcgatattgg ggatgcagaa aacgatattg
    13921 ttgaatgggg agagttcgat gtaaatatca ctgcgaaacc attcgcaaga gttcaagaag
    13981 atgtacctat aaccgtagat aaaccacagt catttaactt gctgaataat agtttagaag
    14041 aaagttttcc aaagattatc atcactcctt cagctacttc atgccagttc atcttaaatg
    14101 attatgtgtt tagttttgaa ggcttagtag taggaactga tgtagtcatt gatagtgatt
    14161 taatgctttg ctacgaagag caatcggacg gagatatttt agatcggtcc aacaaaatga
    14221 agaccatgca atatccaacg ttgcaagtgg atattaatca ttttacttgt actggtttga
    14281 gcaaaataca aatttatcgt aatgggttaa ggtaggtgaa atagatgatc gataatttac
    14341 taactattta cgataaaaat gacgcgaata atttagctga acatttatat gatacgcaag
    14401 gtttaggcgc tttgtcagac tggttaacag ctactgttag caataaacta aacggagccg
    14461 agatatttca gggtacttat tcaataaacg gaactaatgc agatttgatt gtagaaggac
    14521 gtattattca gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg atttattatg
    14581 caaaaacttc tgtaataggc aatacgatag aagtaaaagc tgaacctatt ttcaatgata
    14641 taagaaaatc ggtgttgaat aaatatgaca gcggaacaga aaaaatcact gctactcagg
    14701 catggcaaaa cgcaaaagtt ttagcgaaac cagctatccc ttcgcagttt tctttctcgt
    14761 cattagtaga tacgcttgct aatgtgaaga tagaaaaggc gaatttttta gaattctttg
    14821 gtggaaaaga gggatctatt ctagatcgat ttcatggtga gtttctaaaa gataataaca
    14881 cattacgtca tgaaaaaagt ctaggtacgg atcataaaat caaagcgatt tatactaaaa
    14941 acttaactgg tcttgactta gagattgatg ctcaaagtgt tttagttgga gtttatccat
    15001 ttattagcag ctcttcagaa ggagaagacg agatcactct accagaagaa gttattttca
    15061 cggattacgt ggatgattat cctgctggat atgtttcttt tgttgatttt aaagacaaag
    15121 cgactgatgt agccacatta agggaagctg ctaaagactg gttgaaaaca aacatagata
    15181 aacaaaaacc acaagtgagt ggttcgattg aattagtacc attgaggcat caaagaggct
    15241 atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga cggagtagat gtgtatcatc
    15301 cacagttaaa agtgaatatg tcagcgagaa ttgtggaata tacgtttaat gttctaacca
    15361 attcatacga taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa aacacagaga
    15421 ataatgtcag caatttgatt aatgatgcca ttgatcaatt gaaaaacgtt ggcgaaatca
    15481 gtgatttaat caatgatatt gtagatcatc aaactgatat gattactggt caaaatggtg
    15541 gttatgtttt attagatcct aaagaagcgc ctagtcgtat tttgattatg gacacaccag
    15601 ataagaatac cgcaaggaat gttttacaaa tcaacaatgc tggtattggt ttttctaaaa
    15661 ctggcattaa tggaacatat gaaacggcat ggacgttaga tggcggattc aatgcctcgt
    15721 ttattacggc tggtgaaata gtaggaatta ctattagagg tactacatta attagtgatg
    15781 gtgctgatta tagaacaagt attgctaatg gcaaaatgac ttggtactca aaaaaagtta
    15841 acaaagatat tatggagcta gaagcacgtg attatgtaag tgctgatgcc ggtattgtat
    15901 catacaccat gaaaactggt ggtggtttca tgattagaaa tccacagggt aacttggttt
    15961 ttagtacgtg ggataatggt aataacagac cgtttctatc ttttggtgcg cccaatttca
    16021 ggtatagcaa tgctagttat gtaacttctg gcgacggtag ttctttaagc attaatggta
    16081 gtgcgggtaa ctcatgggaa tttaaggtag ctggtaggac tatgaaattt actagtgatg
    16141 gtatgctaac gttaccaggt tgtttttttg gttcatggga agatgggaaa cttgctaggt
    16201 ttgaacaatc aacggtacaa gtatataaag attttactgt tagaggtact aaaaactcaa
    16261 ctgtaccaac agaacattat ggacaacgac tattgaacgc ttatgaaact ccagaatatt
    16321 atttcgctga ttatggggaa gccgttacag gtgacgatgg taaagttcgt gttgatattg
    16381 accccatgtt tgctgagaca gtaaatctaa gtcggtatat gacacatgtg acacctacag
    16441 aactagtttt gtgtgctgtt actcatgaag atattgacca tttcatcatt gaaactagta
    16501 agccaaacgt attagttaga tggaatttag tggcacaccg tctagggtat gaagatatta
    16561 gattaaaaga ggatacagca tatgatagca cagtgcttga ccaaaaacgt ttttaaaacg
    16621 aagacaagga ggtatataaa tggctagcag tttatataat ttggctttag atttcagcaa
    16681 agaattaaac tacaccaaag ctattatggc tcgtcagggt gataaaggga ttacggtgac
    16741 ggttaaacca tatttaaatg gcttgcagat ggatacgagt ggcggaacat ttactttaaa
    16801 aggaacaaca ccatctaacc gttacgtaga taatgttgca actagcgtaa ctagtgaaga
    16861 agtcacgttt tctcttgatg gcacatttat gagtgaagca ggatattata aacactgcta
    16921 cgtagaatat agaaaagaca atcaaattct aacaacgcaa gatatcattt ttttctcact
    16981 aggagtgtct gacatttcgc aaggtcaagc cgatgaatat gtttcgcaat tagaagagtt
    17041 gattcgaaag tataatgaaa cttttgatgc ttttatggct gaaactaaag gtagagtgga
    17101 tagcttaaat caacagatta ctgatttaac tggtcaagct aaaacgctac aagacaagtt
    17161 agatgctctg aaagaagaaa tttctaagtt aggtaactta caagtgatgt acagtaacag
    17221 catcgatttc gggaactatg attatagtgg gaatcctaat ttgttaagta agctatcata
    17281 cgacttaatt gaaaatcaaa atacttcagc tggaacactt tctaaaggtg aaaactcgtt
    17341 taaatataat aagatatcag ctgaaacgga aggtggagta gagttatatt ataaacgaag
    17401 aggtatagct aactggttac cctctaataa aacgcttgta atgaccgtta aacttagagc
    17461 tggagttgac tatagtccag ctgacggaaa aaaaatactg attagatata ggtttgttga
    17521 caatggaact ggcaagattg ttttagactt acctattaac agtaattcga taactcagga
    17581 atggaaagag tttagtatta ctggaactac tccaacattt ggcccgcaag cataccatcc
    17641 ttggatacaa tttagggctc aagatgggat acttggggaa atagaaatga gctatgacat
    17701 caaaatcgaa gaaggtccaa cagccacacc gttccaacct aacttattgg cagaacctta
    17761 taacatgtgc cgcgagtatc ctaacgagaa cattgtcaat cctacagtta agttcccaat
    17821 taaaacatca aataatccta tttctggttt tgatatttca gaagaattaa taataggtga
    17881 aacttatacg gtatctttaa aagggactaa gccagctact aaagaattcc acttatacta
    17941 cggagaagca aattaccagc agttgcagca tcaggcaact ttacttcctg tggaaggatt
    18001 aacagatgtg tggagtgcca cttttacagc cagaaatact agtgaaacga caaatttact
    18061 tagagtagcg ttatggcaaa aacctaattc tgcaacatat gatactgttc aaattgactg
    18121 gctcaagatt gaaaaaggca acacccgaac gcccaatatt agtgagtata aatatcgtgg
    18181 tactggtatg cgtgattcaa acaatccaaa agattatgtt tgggatctag caccagaata
    18241 tgtcgaagat aatttggcca cagatattaa aatttctgaa attactggta aagcaaacaa
    18301 ttataccgat gggaaagtat cggagattaa ttcgcagttg actgcttcaa ttaatgaagt
    18361 agacaccaca gctaaggatg ctcgaacaaa agcgaatgct aatgcgactg ctatagatga
    18421 attagacaat aagatcgatg aacgcattaa tgatgcagct actaccgcat tttatggcgc
    18481 acaaggtgaa gtctctaaca aaacgaaagt agcggagtat ggagtaggaa cagggtatac
    18541 tactacagct gtaggagaaa cacatttcga acgtcaatct aacggatacg ttaaatgctt
    18601 aaaagctggt aagtattcaa ttagtgcgca aatacgtgtg caattgggcg ggaaatatgc
    18661 ttcatggtta tatactgatt tatataaaga tggaacttct gttgataatt tagtatctta
    18721 tggggtcgac gccttgcaaa atagatttgc agctagcgga aacattgtaa ctgatttaga
    18781 agtaaacgat gtactaaata tgcgtactga aatcggcgtt gcagatggaa ttttacaatt
    18841 tacatcttgc cgtactttag tcttgactag actatctgat taatacaata tttataattt
    18901 tgctatcaac acgctcaaag gagggtgttt tttattttgc aatgaaagga ggctagttgg
    18961 ttgaaagacg aagcaataca agacgtggta gaacgcttag tgcgtattga aacgaaactg
    19021 gataattacg aattattacg tgaaaaagcg gaaagtgcaa aagatagagc ggatcaggta
    19081 tattctattg cgcttaataa tgcagaagac atcaaagaaa tgaaagccaa taataaatgg
    19141 tcgtggggtt acatgattgg tttaggcatt acgatcattg gctatttctt gactaaattg
    19201 taaaggaggt gagaagaaat gattttacca gataagtatt atcaagtcat taaatggaca
    19261 gttttaacag tattgccagc tgcatctgct ttagtagcca tgttagggaa agcgtatgga
    19321 tggaatggaa cagatatgat agtgctgact atcaatgcag tagcaacatt tttaggcgtt
    19381 atcactggtg tgtcggctta taatttgaaa aaataggagg aaacaaatga aaaagaaaat
    19441 tactattact gcgatgagcc tgttaacggc tcttttttta ttgccaatta acggatttgc
    19501 ctatactatc aacaatgaat ttaatttggg cgcaaatgaa ggtagctcac aagtagcaaa
    19561 taatcagtac attttactgc atgaaacggc taatgaaaca gcaacaggac gcaatgaagc
    19621 gcagtatatg caacgttcat ggactagtgc ttacactgct tacattgtgg gtgatggcgg
    19681 aattgtttac caagttggac aacctggtta tgtacagtac ggtgctggtt cgtatgctaa
    19741 tgccaacagt cctgtgcaga ttgagttaca acacacacat gataaagcaa cgtttgagaa
    19801 aaactacaag gcatacgttg aattggctag agattcagca atgaaatatg gtattccatt
    19861 aacgttggac actccttata accaaccggg aatcaaatcg catttatggg taacacaaaa
    19921 catctggggc gatcatacag atccttatgg ttatctttct gaaatgggcg taagtaaaga
    19981 aaaattagca tatgatttag ctcatggatt taccgatgaa aatccgacaa cttcagatga
    20041 taaaccagtc attgatccaa ctagagcagg tgcagcaaat cctacgctga cagatggaac
    20101 aaattacgcc cacattgatc agtttgggga aatcgaaaac gcaaacttgc atgtggctgg
    20161 atggcacatt gctaactata aatacgagta tatcttcatt atggactaca atactggaaa
    20221 agaactagct agagtaagag ctgatgggat ttatagacca gacgtaaatc aagcttataa
    20281 tactttagga aatgttggct atcatgtatc tttcaatatg cgtaattttc ctaataagaa
    20341 agtctatgta atgatgcggg caacgaatga tccagaaggg aacactaaag gcggagcaca
    20401 agattttcat gataaacgct ggtacttgaa tattcctaaa cgataaaaat agcccctc
//
LOCUS       DAGCWU010000035        20454 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.035, whole genome shotgun sequence.
ACCESSION   DAGCWU010000035 DAGCWU010000000
VERSION     DAGCWU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.035"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            242..742
                     /locus_tag="MBZ71_001017"
     CDS             242..742
                     /locus_tag="MBZ71_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBT4428988.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(825..1376)
                     /gene="lepB"
                     /locus_tag="MBZ71_001018"
     CDS             complement(825..1376)
                     /gene="lepB"
                     /locus_tag="MBZ71_001018"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBT4428989.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1528..1755)
                     /locus_tag="MBZ71_001019"
     CDS             complement(1528..1755)
                     /locus_tag="MBZ71_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBT4428990.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(1752..2270)
                     /gene="msrA"
                     /locus_tag="MBZ71_001020"
     CDS             complement(1752..2270)
                     /gene="msrA"
                     /locus_tag="MBZ71_001020"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBT4428991.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2290..3003)
                     /locus_tag="MBZ71_001021"
     CDS             complement(2290..3003)
                     /locus_tag="MBZ71_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBT4428992.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3006..3902)
                     /locus_tag="MBZ71_001022"
     CDS             complement(3006..3902)
                     /locus_tag="MBZ71_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBT4428993.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4060..4902)
                     /locus_tag="MBZ71_001023"
     CDS             complement(4060..4902)
                     /locus_tag="MBZ71_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4428994.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5034..5687
                     /locus_tag="MBZ71_001024"
     CDS             5034..5687
                     /locus_tag="MBZ71_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBT4428995.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(5750..6277)
                     /locus_tag="MBZ71_001025"
     CDS             complement(5750..6277)
                     /locus_tag="MBZ71_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4428996.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6297..7244)
                     /locus_tag="MBZ71_001026"
     CDS             complement(6297..7244)
                     /locus_tag="MBZ71_001026"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBT4428997.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7256..9142)
                     /locus_tag="MBZ71_001027"
     CDS             complement(7256..9142)
                     /locus_tag="MBZ71_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4428998.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(9302..11380)
                     /locus_tag="MBZ71_001028"
     CDS             complement(9302..11380)
                     /locus_tag="MBZ71_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBT4428999.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11393..11668)
                     /locus_tag="MBZ71_001029"
     CDS             complement(11393..11668)
                     /locus_tag="MBZ71_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429000.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12030..12485)
                     /locus_tag="MBZ71_001030"
     CDS             complement(12030..12485)
                     /locus_tag="MBZ71_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429001.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(12639..13847)
                     /locus_tag="MBZ71_001031"
     CDS             complement(12639..13847)
                     /locus_tag="MBZ71_001031"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBT4429002.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(13959..14291)
                     /locus_tag="MBZ71_001032"
     CDS             complement(13959..14291)
                     /locus_tag="MBZ71_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBT4429003.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14439..15314
                     /locus_tag="MBZ71_001033"
     CDS             14439..15314
                     /locus_tag="MBZ71_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4429004.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15396..15977)
                     /locus_tag="MBZ71_001034"
     CDS             complement(15396..15977)
                     /locus_tag="MBZ71_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBT4429005.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(15983..17242)
                     /locus_tag="MBZ71_001035"
     CDS             complement(15983..17242)
                     /locus_tag="MBZ71_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBT4429006.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17244..18290)
                     /locus_tag="MBZ71_001036"
     CDS             complement(17244..18290)
                     /locus_tag="MBZ71_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429007.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(18525..18800)
                     /locus_tag="MBZ71_001037"
     CDS             complement(18525..18800)
                     /locus_tag="MBZ71_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBT4429008.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19043..20353)
                     /gene="der"
                     /locus_tag="MBZ71_001038"
     CDS             complement(19043..20353)
                     /gene="der"
                     /locus_tag="MBZ71_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBT4429009.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT         5697 a         4220 c         3468 g         7069 t
ORIGIN      
        1 caaaggcgga tgatacgata agtattctcc gcctctattt tttttattcg tacaatctct
       61 tttttacagt ttataaaagc agtcttctca cccttttttt aattgttaga tgccagtgta
      121 tgtaaatcac caatgtgtca tgacacattg gtgattttat ggtatccttt tattattaac
      181 tgacaaagga gagaatattt tgaacacttt gattgcaaaa gacgttttta gattgcccga
      241 aatgaatgca agattgttgt cgacctctgc tcttttatta gcccttgatg tcatcttata
      301 caagatagcg attggtcctg cttattttca aatcacattc ggatttttaa gtatggcgct
      361 tatgggttat ctatatggac cgatctgggc tggtttgctt gaaatgttgt cgaacacttt
      421 aaatttcacg atatttggtt caggaacgtt tcaagctgct tttcttatca cggctttcct
      481 tggtggattt attaatggct tatttttata tcataaaaag ataaacctgc cttccgtcat
      541 cgctgcccaa ttattgatta tgatcccagt cagccttttt atcaattctt ggctgatgtc
      601 tgttttgttt ggtgcagatt tcaaagtaat ttttctaggt agattcatca ggaatatgat
      661 ccaaatccct attcaaataa tggttgttta ttcatttatc aaaactttag aggctagaaa
      721 agtgtttaaa cgagctgttt gattttacgt actaaggtag taaactgatt ttggattaaa
      781 caataaagcg tgcagtctca acagactgca cgctttattg tttctcactg gatccatttt
      841 atttcatcaa gtggataata gacaaatctt gctttaccta gaatatcagt tttattgatc
      901 gtgccaaacg aacgactatc ttttgaaact cttcgattat ctccaagtac aaaataatta
      961 tcttccggaa gcttggattg tcccgttagc tcttgtagtg taaagtccgt cgtataggat
     1021 gcatttgcat gatctgattt caggttttta gttagataag gttccttaac tactttccca
     1081 ttgatcttca attgatcatt ttggtagctg acattttctc ctggaagacc gattactcgt
     1141 ttgatataaa tcgtgccatc tgctaattga aaaacaacaa tatcaaatcg tctgatctcc
     1201 gaaaattttt ccatcacaac catatcacct tgcttcaaca cgttttccat tgaattgcct
     1261 tccactggta ccgggatgaa taaaaatccc cgcaacatga acgcaaggaa tacagaaaca
     1321 agtaagtatt tgaatacaag ccagaagcga ttaatatagc gttgtttttt tgtcaatctc
     1381 tatcaatcca tccctctttt tttcattctt tctttattat aagaaaaata tcaaaaaaat
     1441 gcgactagat agctgaaata aaaataattc tttcaattag gctattggac aaaaaacttt
     1501 tttgatcata aagtacaaaa ctatctctta tatgtcatga ttattttcta aatatttttc
     1561 ccatgcttcg tcaaagatat ccatggacgg caaatagtcg acattcagtt ccagataatt
     1621 agaaatttct tcgtagctag agctgtgctt aggaaattgg atgtctttac cggcttctgt
     1681 agcgaaaaca gataaagcat catgtgtgcc accgcctcgc agtgtcatta aataatgaaa
     1741 aaagcttcga ttcacccttc gttcctccaa ttttcttcga taaaggcagc ccggcgtgca
     1801 tggtttcttt taaaattttc aggatgcttt ttataatact cttgatggtc ttcttctgct
     1861 agataaaatg gttgggctgg ttcgattttt gtaacgattg gctgttcgaa acgtccactt
     1921 tcagctaact gtttcttact tttttcagca atgatccgct gttcttctgt caaataaaag
     1981 atcaccgggc gataattgtc accgcgatcc tcgaattgtc cgaaagcgtc agttggatca
     2041 gtttgctgcc agtaaagttc tactaattgt tcatatgaaa tcaatgttgg atcatagata
     2101 atctccactg cttctgtatg tccagtcgtt tggcttttta cttgttcata agtcggatct
     2161 ggtacatgcc ctccagtata tcctgacatc actgtatgga taccaggaag cgtgtcgaaa
     2221 ggctggatca tacaccagaa gcagcctcct gcaaatattg ctcgttccat tatgattccc
     2281 cctctaatac tactcatccg atgatttttt attttctttt gtcttgcttt cagtactgct
     2341 ggtgctagtt ttgttggtat ttgagttgtt tgaagcatca gaggtatctt ttggcaaata
     2401 tagactaaac cgaatgtcat catttactaa gtcgatttta tctgctttaa tatacatccc
     2461 gttttgcatt cggaattgat ccaacctaag catgatagtc agatcatctg tattgacttc
     2521 gatccaatct ggtaatttat agtttctctt gatcatattc atcacatctt tgattggcaa
     2581 actcaatgtt ccgatagaaa gacttctagc ttttagttgg acatttccgt tttccatcac
     2641 atacggatca aaatataaat agaaatcgat aggaaaacct agtacttcga aagtaccatt
     2701 gagtagtgct tcattttcca aataaaattt atattcaata tcactatctt tttgatactc
     2761 actaaggaaa aagctgatga tttgattgac tttttcttta tttgaattga tcgttaagac
     2821 aggatctcct tgtctttccg taagagctgg tatttctggg agttctggtt cccgattagc
     2881 aaaaatacgg ctgcctaaaa atacggtcga accaagtact accccaagaa ggataaggaa
     2941 ggcgaatttc cagtaatttg ttttctttgt tttgtttgaa gatacgtcag atcttgtttt
     3001 catgtttatt gatcatcctc cttctctaac cattggtttt gtgtctcgat cagctgatct
     3061 ctcactgcat ttgccatcaa ttgataacct aaattatttg gatggaaatg atcttcttca
     3121 tagagcacat tatttttgat gccatttaga ttttctgtcg tgctagttgt ttgatctgtt
     3181 ccttcttctg tgacacctac ttcctgattc aatccttgat aaagcaaatc attgattggg
     3241 ataaagtagc agttcggcat tttttttgtc accgtttctg ttcctttgtt ccaattgtta
     3301 acaattgtct gcatgtcagt gatctcagga aagttcaaat aaaatggatt gtagatgcct
     3361 aaaatgtaaa taggtgcggt tttattgagg gatcgaaatt cttccaatag ttctgtgaca
     3421 ttttcctgat agtttttcag tggcttttta aaggaattga ccgttaaccc gaataaatta
     3481 ttttggataa ctttcatcaa atcattaccg ccaacagtca gtgtgataaa atcagctgtt
     3541 ttgatattgt ttcgaatttc ttcttttgtt tttactcgct ttaagatctg gtcacttcgt
     3601 tcaccattta caccatagtt ttctgtttca accgcggtca aattataacg gtcttttaga
     3661 tcatttgcga caaggggaac gaatcctcct tgcttggttt gatctcctac tccttcagtc
     3721 agcgaatctc cgatagctat gtagtgaagt ttttcttttt tatttggatc ttctctcaca
     3781 gtagttactg gtttcattag tgggccagct ttcggtatag aagcagctaa acctgcatag
     3841 atgatgatcg caattaataa tgggaacaaa acgaggatca aatgtcgttt cgttaatttc
     3901 attttttcac ctcttgtatt cgattggttc taatgttatc aaaaatttta tcaaaaaggt
     3961 agtctttatg aaaaaagaaa acaattattt tcctaatttt ataaagaaaa agagaataag
     4021 ctgcaagcct ttcaggctga gggcttatcc tcttttcgac taatctacat aatacatgat
     4081 tgcaaacgcg tctttaccag tatgcgtagc gataaccgga ttcgtatgca gtacagggat
     4141 atgcatgtcc gggaataatt cttgcaaacc ctctttgaat tcttgcgcca attcttttgc
     4201 atccgcatgt gaaataccga tttgtcgcac atttggcaat tctttcagtt catttttcaa
     4261 attattaaac catttatcga acgttttctt tcctcgcccc ttagttacag ggattaattc
     4321 agaatgggcg aaattcatca ccacgcgtat gttcaaaaca ctagacagca aaccagttgt
     4381 tcggctgatt cttccacctt ttaccaagtt atctagtgtg gatattccga tgtataattt
     4441 tgtattgtct tgtacttttt tgacttcttc aaggattttc tctactgttt taccttcttt
     4501 tgctaattca gctgctcgaa ttacctgaaa agataatcct tggtcagtgg tatcactgtc
     4561 gataaccgtt actttcgttt ttgttagatt actggcttgt cttgctgctt ctactgttcc
     4621 actcaagcct tttgtcatat ggatcgaaat cacttcactg ccgtcttctc ctagctcatc
     4681 atacatcgag atgaattctc caattggtgg ttggcttgtt ttgggcagat tttttgcttt
     4741 cgccatcatt tccataaatc tttcgccgtc tagttggtca tcatcgggat atactgttcc
     4801 gtcgatcatg atggatagcg gcatgacacg aatgcctaaa gcatcgcgta catttttttc
     4861 catcgtacat gaagaatctg ttacgatttt tacatttgtc attattttct ctctctcttt
     4921 ctttaacaag atgccttatt catgttaaac tacatgtaag acaatttcta tttttagtat
     4981 aacagacaaa taatgatttt tcattaatta cacctagtaa aggagccgtt ttcatggaaa
     5041 aaacgaaatt taatcgaacg tatgaaatat taaatgaagt gtttaatgca gtcacccatg
     5101 gaattgggac tggattgagt atcgcgggac tagtggttct tttagtaaaa ggtgcccact
     5161 tgcattcctc cctccatatt gtctcttatg ctatctatgg atcgatgatg attcttttat
     5221 ttttaacgtc tactttgttt cattcattga tttttacaaa agcaaaaaag gtgttccaag
     5281 tattcgatca tagttcgatt tttcttttga ttgcaggaag ttatactcct ttttgtttgc
     5341 tgagtatccg aggatggcta ggttggactt tatttgtatt aatctggttg tttgcgattc
     5401 ttggcattat atataaagcg ttgacgttgc ataaacggga gagagtatca aaagtatcta
     5461 cgatcatcta tatttttatg gggtggctct gtgtcgtggc tgctgttcct ctttatcact
     5521 ctcttggaat gactggtgtt attttgatgg tagcaggcgg gctttcttac acgataggcg
     5581 cattctttta tagcttgaat tctatccgct atatgcatgt tgtttggcat ttgtttgtta
     5641 tgttaggtgc aggttttatg tacttctctg ttttattctt tacttaataa aaaaaataaa
     5701 gctatggatg ggacagctct aaataacagc tagtctatcc atagcttttt tatatcgtca
     5761 attcgcctgt cccgcttttt acaggattct cattacgctg atacgtttca aattgatgcg
     5821 gataacgatt tttttcatcc atttctcctt gactagtatt gatcagtgac caatcagacc
     5881 agtcaacagg tggaaaataa gcatctccat cgaatacatg atggatgact gtacggtata
     5941 atatcgtaca aaatggcaga aattcttgat aaacagctga ccctccagcg atgaaaaaga
     6001 taccgtctgt ttctttttcc agagcaagca cgtcttccac actatgcatg acatgcacgc
     6061 cttctgcttt ataggaagaa tctctcgtca aaacaatcgt ctgacgattc ggcaacggac
     6121 ggctacccat cccttcgaat gtttttcgtc ccatcactag tgtatttgct tctgtcattt
     6181 gtttgaagaa tttcagatca tttggcaaac gccaaggaag acggtcttct tttccaatca
     6241 atccgttttc atcctgtgcc caaattgcag ctaacatttt ctcatccttt ctgattctaa
     6301 acagcgatag gagctttgat cgccggatgt gggtcatatc cttctaattg gatatcttcc
     6361 atctcaaaat caaatactga ctgcttttcc tgattcaaaa ctaaagtcgg aaatgcgcgt
     6421 atttctcggc tcaattgttc tttcatttgt tcgatatgat tgatataaag atgagcatct
     6481 cctaatgtgt gaacaaattc gccaaccgct aaacctgttt catgtgcaat taggtgagtc
     6541 agcaacgcat aacttgcaat gttgaacgga acgcctagaa acacatcccc actgcgttga
     6601 tacaattggc agcttagccg tccctcttga acataaaatt gaaacatcgt atgacaagga
     6661 ggcaaagcca tgctaggaac atcttccgga ttccatgcag aaacgatcaa tcttctagaa
     6721 tcaggagtct ttttgattgc ttcgatcact tgtttgattt gatcgataaa gccaccgtct
     6781 tttgtttccc agtgacgcca ttgatagccg tagatgtgtc caagatcgcc gtatgtttca
     6841 gcaaaggaat catcggttag gatcttctcg caaaaaagct tgctctgctt tttatattct
     6901 tcattgaatt caggatctgt caaacatcta tggccaaaat cagtcatatc tggtccatta
     6961 tagtctggac ttttgatgta gcgttcaaat gcccattcat cccaaatatg gtttttattt
     7021 tgtaatagat agcggatatt cgtatctcct tttaaaaacc aaagcaattc gcttttgatc
     7081 aatccaaaag gtactcgttt cgttgttaac aaaggaaacc ctttttgcag atcaaaccgc
     7141 atttggtatc cgaataagct taatgtgcct gtgcctgttc tgtcttcttt acgatgccct
     7201 tcttccaaca attttcttcc taagtcaaga tacgcttgtt ccatcctttt tcctcctaat
     7261 cttctaccca ttcactcaag tattcccacc gtgccatttt ttcttcaagt tgtgcttctg
     7321 tttcagatac gtcgttttgc aattcttgta atcgagtgaa attatctcct tgatgattca
     7381 tctcttcttg gagcaatgag attttttctt caagttctgc aatctcatct tcaatcgttt
     7441 cccattcttt ttgttccatg taagataatt tcttcttttc agcaggttcc ttgctctgta
     7501 tttttggttt cgctttttca gcaggctgct gggtctcttt ttgcttcagt aaataactgg
     7561 acatgtttcc gtaataaagt tcttgttttc cgtttccttg gaatactaat aacttatctg
     7621 ctaccttgtc caagaaataa cggtcatggg aaacagtgat cacagcacct ggaaaacttc
     7681 taaaataatc ttccaaaatc gtcaatgttg ctatgtccaa atcattggtc ggttcatcca
     7741 ataataaaac attaggttgc tgaatcagca gtttcaacaa aaataaccgg cgtttttctc
     7801 cgcctgaaag tttgcctatc agtgtcccat gcataaatct tggaaataaa aatcgttcaa
     7861 gtaattctgc tactccgatt tgactgccat ctgctgtttt tgcttcttca gcagcttctt
     7921 gtaagtaagc aatcatccgt ttatctggtg ccatttcttc attttcttgt gtataatacg
     7981 caagatgaac cgtttcaccg atagacattg taccgctatc caggggaatt ctgccagcta
     8041 gaatattaag caatgtcgat tttcccgcac catttttacc agtaatacct agtctttccc
     8101 gagattgaat caataaatct aaatgttcta gcagtgtttg gtcatttata cggtaactgc
     8161 catctttgat ttccagtact tttttcccta aacgttgtgt cgctaggttc agctctaaat
     8221 catcttcttg gtttacttgg tacaagttct cctttagatc ttcgaaacgg ttgatccgtg
     8281 cttgctgctt ggtgccacgt gcctgtacgc cagccctcat ccaagctaat tcctgtttat
     8341 aaagccgctt gcgcttttct tcttgttcta cttctacacg atcacgttct gctttttcta
     8401 agacgtaagc ttcataattt cctttatact cgtataattt tccaaatgaa agttcgaaga
     8461 ttcggtttgc tactctatct agaaaataac gatcgtgggt aaccatcaat agtgcgccac
     8521 ggtattgttt caaatagttt tccaaccact cgatcgcttc ataatctaaa tggtttgttg
     8581 gttcgtctag taatagtaaa tcgggttcat caatcaacac ttgcgccaga ctaactcgtt
     8641 ttttctgacc acctgataaa gtactgattt ttttatctaa ttcactgatt cctaattttt
     8701 gcaaaataat ctttgcgttg gtatctgtag tccaagcatc ttctttattc atttgttctt
     8761 ctgctttagc ataacgtttt tgcacatcag ggtcttctcc gttttcccct agttcgatca
     8821 atgcttcctc atatgctcga acagtttgga tcaaggggct gtttccttga aagactgctt
     8881 ggagaacagt taattcatcg gaaaattcag ctgcttgaga aagataacca atacggtaat
     8941 cagttggata aaacactgtt tgccgatcac cgtcgctgct atcgataccg gcaataatat
     9001 ttaagagact tgttttaccc gtcccgttcg tcccaatcaa cccgatacgg tctttttcgt
     9061 ggaccaaaaa agaaatctgg tcaaacaaat ctttttcacc ataagttttt ttgaggtcag
     9121 ttacttttaa ttctttcaac atgttccctc cttatcttgg atattgtatc ataaaaatag
     9181 tgaacttcag gaaaattttt ttggtatttg ttctttttgt gttctcaaaa aaagaacagg
     9241 accgattcga tcctgttctg atatgttttg ttttttataa aacttgaaaa tttttacctt
     9301 tttattttat tggactcgtt ttttttgact ttgctcgaaa cgcaaatcgg aaagaaaagt
     9361 tatcagatgg tttgttcatc gtataagatt ctaacaaatt gatattcctg cgatagctgt
     9421 tcgcagaaaa cgtcgtgatt ttttcagcaa cttcgtattc atcaatgatt tttctttcaa
     9481 gataataacc tcttagcatt tttttcgtaa acagactatc ttgctggatc atccacgaag
     9541 aaatcaaatt ccccgagttc atttttattc cttcaagttt gcgttgctgg ataaaaaatt
     9601 gaaccatcgt ttcatcataa atatcagtca agttatctaa actttcaata atcaactgag
     9661 tatttttttg atatgtctct ctgatttctt ggatatcttc atcactaata aatgtctgac
     9721 gatcagcata tcttctttgc caaaacattt tcggatggag aacgattcgc attaacctac
     9781 ggacaacaat gaaccaaaag ccgataaaag acaaaatctg gcttgtgata gaatgttcga
     9841 atcgagaaag aaaacgggag taaaaacgat aaccattaga agaaagcttg cccgaacgat
     9901 aactttcatc caatccatcc cgttcgatcg acaaaatcag tgcttgtatt tcttgtactt
     9961 cttgcttttc atcatcggtt aaatcttcca agtacagatc acgtatccgt tcttgataat
    10021 tttctatcac tgcttcagtg gctaaaaatt cttttgtatt acgatcaatt tcctttattt
    10081 cttttcgcaa aacttcaacg acctcttcta aaatcatcaa agcattcaaa tccgtgcttt
    10141 tcgcttcctc tgtttccgtc agcataggca agacaatgat ccctatgacc aaagtcacta
    10201 agatgacacc cgccgtcaga aacaaaagca aagaccgctg gtaaaacatc atattattga
    10261 ttgaaaatgg caaaataaaa atcgtagcca aactgactgt tccctttaca ccgccaaatg
    10321 ttaagatcaa gatctcgttc aactgttttt tgaacttttt actgccatct ttgaatacat
    10381 aaaaaagact gatagaaatg aatctgatca caaaaagcat gacagagatt aatacgataa
    10441 tcgctaataa aagaccgttt ggatataaac cgtctcccca tactggcgag aacacttgcg
    10501 tcaattcgat tcctaaaaat ataaatacta atgcattcag tgtaaaagca atagttgtcc
    10561 atgtactgtg ggacaagctc gacaattctg catcaaacac agagattttc ctaaaaccag
    10621 aagcttggag aatacctgct gccactgcag caatgattcc agatacatca aatgcctctg
    10681 ccaatacata ggctagaaaa ggaagcaaaa gttcgatcag cagataccct gtgacatctt
    10741 gtgctgatgc tttttcgatc aaatgaatta ttttttgttt gaaccagaca agcaaaaagc
    10801 cgatcaaagc tcccccaata ctagaaacta ctagactcat ccccgcgttt actgctgaaa
    10861 aagatccggt gatcagcgca ccaagagcaa actggaaggc agtaacacca gatgcatcat
    10921 tcaaaagacc ttctccttcc aaaatatgca tcgccttttt agggatattc actcgtccag
    10981 aaagagaact gactgctacc gcatctgtcg gacctaacgc tgctccaaaa gcaaagcagg
    11041 cagcaagtgg gacacttggc aacaggaatg ataacgtcgc acctaccccg acaagggtca
    11101 aaatgactcc tccaaatgcc aagaacaaaa ttgtcccgaa atttttgaga atcgaagaaa
    11161 tatctgcttt ttctccttct ctaaataaca atggtgcaat aatcataact aagaacaatt
    11221 caggttcaaa atcgattgat tgtccccact ctgtcagacc taataccact ccaaggaaaa
    11281 tttggatcag tggagctgga atcattggca aaatgcggct agctatgttc gataaggtga
    11341 tcgttactgc aaatacaata actaactcag caaattccat cgtccatccc ctttattttt
    11401 tattttgtgt aagcattttt aaaaattgtt cttttttctt taaaattttg gtttcgacta
    11461 cttcaacctg tgtttggttc ccctctaatt cacactgtac acgttttgac tccaaacgaa
    11521 gcagatcatg tttcagacgt tccaactgac gatacatcaa tatatttctt ttacgcaact
    11581 tacaaagtat ttgacggcgg tcatccatcg aaagggcgcc atagcgatca tgcaacagtt
    11641 ttttttgttg ttcaaaactc tctctcattt tccagatctc ctatacacaa acttccatca
    11701 ttatagcaaa gttctttttg gatgaaaaat atttcattgt ttcatcgttt attgctagtt
    11761 agaaatttcc ataaattttt caacgcctca ttctattcca aatagtatca gatatcaagt
    11821 aaaacttaag aaagataaaa gtttctgaca aaaggatgtt tactttccca ttcctgcctt
    11881 tcttgttctt ttatcacaac aaaaaaatgg ggctgcgaca aaaatcttgt cacagtccct
    11941 tattctgaat aaacggtgtg gaagagacag cctatcagta gtaataagac acatctcttt
    12001 cacagcctca tttttgcgta aaaatcgatt tattcgaaaa attccccgta tacatcttct
    12061 acacgttgat ctggattgat cacgatatac aagttacctg ttttttcgct gatttttgtt
    12121 gattgatatt gattgtctaa tccttctgtt cctttatcta gatatgggaa gtacacaaga
    12181 tcaggtgtgc catttcggta aaggatttcc attcgctcga tatcttcgta tttcaatgcg
    12241 cgattaaaca tgcccatttc cagtccgcct aggttgatat catgtgtcgc aactgcgtcc
    12301 ccttcatgga agatttcgat tttaaatcca gcacacggat ggatttcaac aaattcactg
    12361 ccattgattc gtccaaaact tgttgtgatt tgtttgatcc aaagatcgcc gatatgacgg
    12421 cggttgatcg tccaggtttc tccatttcta aaataaaatt ttagcgctgt catttcttta
    12481 ctcattaaaa agactccttt atgatgtaat ttattcttgt actacctcag tttagcacaa
    12541 atttatgtaa gtggtcacat cttatgtttt ttcttctaat gacgtatctg cccactttaa
    12601 gtagccaaca aagctgagca gatacagtct agtgtttttt aaatcaattt ttctttcgat
    12661 aaaaaatcca aaacctgttt tttatcattt tttattcttt gttctacgac ggctttttca
    12721 gctaattgca aagcttcact aacccactta ccaggttttt ttccagtttc ttctagcaag
    12781 attttaccat ctactgctaa ttcgcttaat gacttgatag gtaaacgatc ataggattct
    12841 cctacggcat ccatctgagg tgtttcgtca aaatagatca tcaattcttc tacttgcagt
    12901 gcatgttttt tgcctaagaa gtacaattcg aatggttcca aagctccagt ttctcgttgt
    12961 cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt ggttagaaca cttccacgat
    13021 ttcaaaaagg aacgaatctc attttctttt agatccagtt ggctgatcaa taatgcccat
    13081 gcttggctct cttctttcaa tgggacggat ggtaattcag caaaacgtaa aagtgcttcg
    13141 ccttcttctt tcaaaccggg acagcaaatg tagcactctg tttcaataaa tgcatggatt
    13201 cccgcggccc gttcatttcc caagagcatc ttgatgaatt ccacatttat acgttctaca
    13261 gatattttac ctaatagctc gtggttttct ttgatggcgt agaaggtttc ttcctctaat
    13321 ttgaacccta gttgactgac aaaacgcaac ccgcgcatca ttcgtaaggc atcttcatgg
    13381 aaccgctcat gaggattgcc tacagctcgc aagatctgtt cttctagatc ttgcagacca
    13441 tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa acgcattgat cgtaaaatct
    13501 cttcttttca aatcttctgc caacgaacga acgaattcga catgatctgg tctacggtag
    13561 tcctgataag ttgattccgt ccgaaatgtg gtgatctcat attgttcatc attttctaat
    13621 accaacacag ttccatgttc gatcccaata tctatcgttt ttggaaaaag ctgtttgatt
    13681 tcttctggaa atgcgcttgt tgcgatatcc acatcatgga ttggctggtt cagcaagata
    13741 tcacgaacac ttccacctac aaaataagct tcatatcctg cattctctaa ttttttcaga
    13801 actggaattg ccttttggta ttccagaggt aattgtttta atttcatttt tccaaccttt
    13861 cctgcgtgtt tcttgtcctg agataaagac tcagttgcat ggtgactgtt tgacaagtcc
    13921 gtttcagagt cttcaaggcc aggaccactt catcttgttt actcgtcctt acgctcaaag
    13981 cgattatggt ctctttgatt gaatttttcc atgttttttt caaaaacttc agttaagtcg
    14041 atattcaatg aattagccat gatcatcgta acgaataaaa catcaccaag ttcttctgct
    14101 accgagttaa ctggttcaga agcctttttg cttttttctc cataagtgtg gttgacttct
    14161 cttgccaatt cacctacttc ttcagttaat cgagccatct gagctaatgg agagaaatac
    14221 cccaccttga attgctggat atattcatcc acttcctgct gcatactgta taaagaacgc
    14281 tttttatcca tctttttccc tccaaaacta tcttaccaaa actttaacgc aaatcctaga
    14341 taagagcaat tgagaaaatc gaatttccat gctacattta ggaaggaatt tctaaatgga
    14401 aattctgtga aaatacataa ttgaaagctg gaattcatat ggtcaaacga aaaacagtaa
    14461 aagaagttct ttttatcatt ataggaacaa gtatttatgc atttggtctt gtttacttga
    14521 acatcgctaa tcatttagca gaagggggag tctcagggat caccttgatc ttaagagctt
    14581 tatttggtat tgatccagct tatacgactt tattaatcaa catcccattg attttactag
    14641 gaggaaagat cctcggtaaa cgttccttgg cctatactgt tctaggtaca ctttctttgt
    14701 cattcttttt atggatctgg caaagggttc cactagaaat caatttacag catgatctac
    14761 tgatcgtttc tctacttgcg ggtttagttg caggtgtagg aagcgggatc gtctaccgca
    14821 tgggtggaac aacaggcggc agtgatatta tcgctcggat tttagaacaa aattacggca
    14881 tcactatggg gcgttcccta ttggcatttg atatcatcgt cttacttgct tcattaacct
    14941 atatcgactt gaaacgtatg atgtatacgt tgattgtttc gtatgttttc agtcgtgtga
    15001 ttgattcgat cttagatggc ggatattctg caaaaggtct acttgtcgtc tccaacaaaa
    15061 gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag aggtgtgact ttcctttctg
    15121 gagaaggcgc attctctggt aattcaaaaa aaatcatcta tatggtcgtt agctctagtg
    15181 aattaactga agctaagagg atcgtccacg agattgacga aaaagctttc ctttcaatca
    15241 tcaatgtgca tgaagtggaa gggcaaggct ttacttattt gaagccacag acaagattac
    15301 tgaaaaaact ataagaaaaa agacaaagat aggctatgag gaaagattcc acatccagct
    15361 gacctttgtc ttttttagta ctatactatt tttaactaat tgttatgttc acttacccgg
    15421 ttacttttct tttgtgtctg caaaaggatc tttcggttaa cttcatcaga taatttcaaa
    15481 aaagcatccc tatctccatc ttctagtgct tgatcgattt gagcataaag catttttagt
    15541 tttgcctctt cttctttttc ttgaaaatat cgttcaatta attcagtatc ttcttcactg
    15601 actgaagaat cccaagaaga aaatggattg tcttctaaga tagatagata ttctcttgtc
    15661 tgccaagcat tttcgaatat acattccaca tataaaggct ttttccaatt caaacggatt
    15721 tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct gcaaaaacaa tttcatgggt
    15781 tccccttcaa cagatagatc actgatctgc agtcctcgat ccgcaaaatt cgccccttca
    15841 acaaagtgga cattgcttaa aatcgcttca tgattggcaa gataattcaa gatccaaaaa
    15901 acttctcttc ggctaaaaga tacatgattg accatccacg ttaaaaaatt cttcttttcc
    15961 cgtacatcaa ttagcatttt tctcacctct ctgttaggtc atctaataat gacagtgcct
    16021 ccatatctcc tggttcaagt gttaaataat aagtcaataa ttcttgcgct cttttcagct
    16081 gcccttcttc tcttaagaaa aaggcatact ctttcaagaa atctggttca tgtgatagtt
    16141 catgataagc ctgttcatag tgtacagctg caacggtaaa gtcttctaat tcattataag
    16201 catgggctag gttccattct gcataaggat tagcagtttc ttccatctga ttgatcactt
    16261 gtatcacatc ttcatatctt tcatcatcca agtaaagatt acttaaagta agtaatgttt
    16321 catcttgttt gtctcctact tctaaagctt tcagtaaaag ttcttctgct cgctgtctat
    16381 catgcagacg gaaagcattt tccgaggcca ggtgataaag ttcgacttgg taaggatttt
    16441 ctttgattcc ttcttctaac actgtttgtg cttcttcaat catttcttct tcttgaagag
    16501 cttgtcctag atatagatat aacgattgat aatgcggatt aacttctcga agttcttcca
    16561 gatagttgat tgcctgctga ttttctttca attgaagatt cgtaaaagct agctggaaca
    16621 gtcgatcatc tgtcctttct tctttcagtg cttcttctaa aggtacgata gctgcttcga
    16681 attctccttg catacttaag gattgtccta aacgttcctg catcgagatt cctgaaattt
    16741 catttatccc gcttgccaac aatgtttcat aaaccactgc tgcctctttg aaacgatcaa
    16801 ctgaaaaata caattcagct agcgcaaact ggatcaatgg ttcatcagga agcaacccag
    16861 ctgcttcttt caattttgct tcactcactt cagggattcc gataacttgg tacaaatcag
    16921 ctatcgccaa taagctttgc ggatagtaat cactttgctt atccactttt tccaggtatt
    16981 caaaagctaa gtcgatctca ttatcttcga ttgcaatttc tgctaacggg atgttcagtc
    17041 cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat ctgttttgct tcgatcaaaa
    17101 agccaatagc cagcaattct tcgcctagtt cagctaatat atctgctggg tcttctttta
    17161 atgctttttc taaaaagatt tgtgcttgtg acaagtcttc atctgataaa gcttgcaaca
    17221 ttttttcgct atttgtattc atttcaattc cctcttatct cactgacgat ttgttgaaat
    17281 accgtcattg ttctcaataa ttctttttcg tttggatgtt ggtatccgtc tattatacct
    17341 attttttcta aaacaagtat ttttttgctt ttttctgcca aaaaaagaac tttttccaaa
    17401 tactccatct ttgaaatctg ttccagccag tggattggat agccaagcaa gtccagccac
    17461 acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt ggagattcca cacgaaatga
    17521 aacatcattc ctaaaaattt tttcatacta gcagaaagta gatggccatt ttcggtcaga
    17581 taaaaagctt gttcaaacag tttaccataa tttgacaggt tttctgcctc taattgataa
    17641 aaatagatca tttcttcgat catcccggca aacggccgag tcgttaattg ctttttattt
    17701 ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac aaatcattcc gcaacagata
    17761 aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct cgctgattgt ttgatcataa
    17821 acgatgcgtt ctggcagatt gatggtttgc agcactacac gatttggtag ctgcaaaatc
    17881 gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg atacagggat catccatagt
    17941 gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca gatccatgat cccttcattt
    18001 ccaaatccga tgataaacat ggattgattt tcgggatagc gtttgacgaa atcaagcagg
    18061 ttctttaatt ctgttaaatg attgcattga gtgttggcac aaatatacca gtcattgttt
    18121 gcttgatttg caaataacgg gttgatctta tctgcaaaca aatcataata gcgctgattc
    18181 gttaaaaata agattggctg atcaatggaa agttctctta gctgactgac tagcgtttct
    18241 ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat caagttccat acgcatccct
    18301 cctcttctta tttttatttt accattagta agcaaattct agaaaagata agagcaaagc
    18361 tcacctcata acttgcaaat aaataaaaat taaataaaat acatgtgttt tctatgtatg
    18421 acatacggat tcggaatctc tgtctcttat ttattccttt tctgctagga aataaaaaga
    18481 aaagcacttg tagccctaga gccacaagcg ctttttcaac tgtcttattt aacagcgtct
    18541 tttaatgctt tacctggttt gaatgcaggt actttgctag ctggaatttc gatttcttca
    18601 ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc gaacttcaaa gttaccaaaa
    18661 ccgattaatt gaactttttc gccgttagct aaagcatctt gaatagttga aaatacagca
    18721 tctactgctg cagttgcatc ttttttagtt aaatcagtag ctgaagcaac tttttcgatt
    18781 aattctgctt tgtttgccat ggatagattc acctcctcca caagagatat gatagaaata
    18841 tcatttagtt aaaactttct gagtggtcca gaagtttaaa aagaaatatt gattaacttc
    18901 aatattctgt tatcacgata tcatatgaac cgcttaatag caaggttttt accaattttc
    18961 acatgctttt tttagtcata gaaacgtaac aaacgaaact gtctttttct tttgaaaaaa
    19021 aaacgtcgat atggtaaaat gcctattttc tcctacgagg gataattcgg atggacgtcc
    19081 cttcaaaagt aaatgctttt ctgatttgat tttctaagaa acgttcataa gaaaaatgca
    19141 tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg tttgacagcg acttgtgtag
    19201 cataaaaaat cttcaggcgt tttcctttat ctgttggtgt tggattgatt gctacagcat
    19261 ccataatgat atcattcaac acagccgaag agatacgaag attctggttc atacttactt
    19321 gttcgatcat ttcaggcaga cgttccaacc gttgtttagt cactgcagaa acaaaaacga
    19381 ttggagcgta atcaagatac cggaattcat cacgaatctc ttgttcgaaa tcacgcattg
    19441 tattggtttc tttctttaca agatcccatt tattcacgac gataataatt ccacgaccag
    19501 cttcatgggc gtaacccgct actcgcttgt cttgttcccg aatcccttct tcagcattca
    19561 gtaccatcaa aacgatatca gaacggtcaa tggcacgcat tgcacgcatc acgctgtatt
    19621 tttcagtatt ttcgtatact tttccgcgtt tacgcatacc agctgtatct atcattaaga
    19681 atttctgacc actttcagaa acgaagtggg tgtcgattgc gtctcgggtc gttccttcaa
    19741 tatccgaaac aattacacgt tcttctccta aaatcgcatt gatcaaagag gatttaccaa
    19801 catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc gtcctcttct tcagtctctt
    19861 cagagaaata cttcactgct tcatctaaaa tatcaccaat ccctagtcca tggcttcctg
    19921 aaacaggata aggatcgcct agccctaagg aataaaattc atagatatcg tttctcattt
    19981 ctggattatc tactttattg actgctaaaa tcactggttt attacttctg tataaaattt
    20041 tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc gctgactaca caaatgatta
    20101 catctgcttc ttcaatcgcg atttctgctt gatgcttgat ctgatccata aaaggttcat
    20161 cgccaagatc aattccgcct gtgtcgatca cgctgaattc tcgacctaac cattcacctt
    20221 ttgcataaat acgatcacga gttactccag gagtatcttc gacaatcgag atacgctcac
    20281 cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg gcggccaacg attgcaattg
    20341 ttggatttgc catattattt cctcctcaca agtttccaat ctcacttagt ttaacgaatg
    20401 cattgccaac tttcaagcat agattgtcaa ttaaacgaag tatttttatt cctt
//
LOCUS       DAGCWU010000036        20409 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.036, whole genome shotgun sequence.
ACCESSION   DAGCWU010000036 DAGCWU010000000
VERSION     DAGCWU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.036"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..217)
                     /locus_tag="MBZ71_001039"
     CDS             complement(<1..217)
                     /locus_tag="MBZ71_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4429010.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTA"
     gene            complement(239..841)
                     /locus_tag="MBZ71_001040"
     CDS             complement(239..841)
                     /locus_tag="MBZ71_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBT4429011.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(1345..1974)
                     /gene="udk"
                     /locus_tag="MBZ71_001041"
     CDS             complement(1345..1974)
                     /gene="udk"
                     /locus_tag="MBZ71_001041"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBT4429012.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(2058..2753)
                     /locus_tag="MBZ71_001042"
     CDS             complement(2058..2753)
                     /locus_tag="MBZ71_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBT4429013.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(2766..3482)
                     /locus_tag="MBZ71_001043"
     CDS             complement(2766..3482)
                     /locus_tag="MBZ71_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBT4429014.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            3812..4417
                     /locus_tag="MBZ71_001044"
     CDS             3812..4417
                     /locus_tag="MBZ71_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBT4429015.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            4742..5065
                     /locus_tag="MBZ71_001045"
     CDS             4742..5065
                     /locus_tag="MBZ71_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBT4429016.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            5247..6617
                     /locus_tag="MBZ71_001046"
     CDS             5247..6617
                     /locus_tag="MBZ71_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBT4429017.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(6747..8048)
                     /locus_tag="MBZ71_001047"
     CDS             complement(6747..8048)
                     /locus_tag="MBZ71_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBT4429018.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(8278..10236)
                     /locus_tag="MBZ71_001048"
     CDS             complement(8278..10236)
                     /locus_tag="MBZ71_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBT4429019.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(10413..10679)
                     /locus_tag="MBZ71_001049"
     CDS             complement(10413..10679)
                     /locus_tag="MBZ71_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4429020.1"
                     /translation="MLNYLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(11189..13315)
                     /gene="pnp"
                     /locus_tag="MBZ71_001050"
     CDS             complement(11189..13315)
                     /gene="pnp"
                     /locus_tag="MBZ71_001050"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBT4429021.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(13503..13772)
                     /gene="rpsO"
                     /locus_tag="MBZ71_001051"
     CDS             complement(13503..13772)
                     /gene="rpsO"
                     /locus_tag="MBZ71_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBT4429022.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            14023..14586
                     /locus_tag="MBZ71_001052"
     CDS             14023..14586
                     /locus_tag="MBZ71_001052"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBT4429023.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14839..15360)
                     /locus_tag="MBZ71_001053"
     CDS             complement(14839..15360)
                     /locus_tag="MBZ71_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBT4429024.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(15363..16205)
                     /locus_tag="MBZ71_001054"
     CDS             complement(15363..16205)
                     /locus_tag="MBZ71_001054"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBT4429025.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(16198..16923)
                     /locus_tag="MBZ71_001055"
     CDS             complement(16198..16923)
                     /locus_tag="MBZ71_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBT4429026.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            17099..17395
                     /locus_tag="MBZ71_001056"
     CDS             17099..17395
                     /locus_tag="MBZ71_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429027.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17963..19555
                     /locus_tag="MBZ71_001057"
     CDS             17963..19555
                     /locus_tag="MBZ71_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4429028.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            19568..20308
                     /locus_tag="MBZ71_001058"
     CDS             19568..20308
                     /locus_tag="MBZ71_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4429029.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5871 a         4205 c         3838 g         6495 t
ORIGIN      
        1 aagcagtgta accgacagtc ttttcttcgc tttttaataa aaagtaggac acaccttctg
       61 tcagttcttt ttgaatctgt ttttttgact gataagtttt cagcatgtaa tccacttgtt
      121 tttgaccaat gatcggcaca aaagcttccg gccatacttc ttgtatgact gccatcagct
      181 catccagttc attttctgtt acttttattt tttccataca cccacgtcct tttttacatt
      241 ataaatgacc ttgcgcttga tggaataaaa agagcatgat catgctatac acacccaccg
      301 accatggttt caagaaaatc acaatctgca taaattccct aaaagataat ttcgtcaaac
      361 cagctactag acagatcaag tcgtctggcg caaagggaag cagcaaggta acaactagta
      421 cttttttaat atctttcgtt tttcttgtca gcaattcatc aaatttctga tacttttttg
      481 gcgataaaat aagctggaca aaggctacgc catagtaacg gacaagcaaa aatccgattg
      541 cttccccaat cactaggcca atacaactat ataaaagccc aaatccattg ccaaacatca
      601 gcatgcccgc tactgaggag atccctcccg gcaaaatagg aatgatcact tggatcactt
      661 gcaacagaac aaatatcatc actgcatact tgccaaattg ctggataaac gcttgcaaag
      721 acgtgctcga tcgaaaaatc ccatgatgat aagcataaag aatcaacaca aagaaaaaaa
      781 acagaccaat aatgggcatg atctgaagac atttttgtaa aactgatcct tttttagaca
      841 tctatattcc ccacatcttt tttcatttat taaaactgcc tccttagggg gtgcgacaaa
      901 agagcttctt caaaaaaata agtcgaaatc tcacacaaat tcgagaaata attttcgtaa
      961 gtttcttctt atttattgca tctgaccact tctgttacaa cctctcgtta aacggtgttc
     1021 aaaagcttgc atctacgata ataagcccaa cccaacgaaa aacgtgagga gctgttttat
     1081 ttggatgctc ttatcgcttg atgcaaagac agctttttta caacctctac ttcacggtgc
     1141 tcaatcagct gaccttcggt attagctcaa aaaacggtaa aatataaaca gctattttcc
     1201 gtttcttctt tttaatactt aggtcaagat gctgttttca caacctcttg ttataaataa
     1261 aaaaacagtt ggctggtgac tgttcaaaaa gcagaatcct tgcttttcaa acggtcgcag
     1321 tccgttcaac tgtttcgctg cgcactattt atggtttaag aaactagcta cttttgttgt
     1381 aatcaaatca atagcaacgt ggttttctcc gccttctgga acaatgatat ccgcatagcg
     1441 cttcgttggt tcgataaatt gatgatacat cggtttaaca actgtcaaat attgttcgat
     1501 cacggagtcc aatgtgcgtc cccgttcttc catatcccgt ttgatacgac gaatgatacg
     1561 aatgtcatca tctgtatcta cgtatacttt gatatccatc aagtcacgca gacgttcgtc
     1621 ttctaaaatc aggattcctt ctagaatgat cacttctttt ggttcctgaa tgatcgttgc
     1681 ctgacttctt gtatgcgcta catagtcata cacgggtttt tcaatggctt tatagttcag
     1741 taagtcgcct acatgctgaa tcagcagatc attatcaaag gcaaatgggt gatcgtaatt
     1801 tgtgttcaat cgttcttcaa aactcaaatg gctctgatcc ttgtaataag aatcttgttc
     1861 aagcatcatg atggaatggt ccgggaagtt gttgaaaatc gcacggctga cacttgtctt
     1921 tccgcttccg gatccacccg tcacaccaat gataattggc ttgctttttg tcattgacta
     1981 aaacctcttt cgtatccgtt ttttcattct aatctattat agcgaaaaaa cacgaaaatg
     2041 ctatattttt ctgaaaatta atccttttca atttccaata atgaataaag ttcctctaat
     2101 gaatcgattc gataagtagg ctggatttct tcgatttctg gtaatttatt tctattgaac
     2161 caaatcgtat cgatccctgc catatttcct cctttgatat cagaagtcaa agaatcacca
     2221 atgatcacag tttgcttttt atcaaaatta ggaatgtggg aaaagacatg atcaaaaaat
     2281 tctttcatcg gcttttgcgc tccgacagct tctgagacaa agatatcctt gaagtaatca
     2341 agcattcctg catctgttaa tcgccgatgt tgcgtgtgcg acacaccgtt cgtcacaata
     2401 taaagatcag catgcggatg gacctttttc aggatttcta gacttcctgg aacaagatca
     2461 tgaccttggt tcaagtagtg gcggtagcgt ttttccattt gatcactatc tactgttttc
     2521 ccaaattgct ggaaaagtaa cccaaaacga ctgcctatta tttcttctcg tgatagtctg
     2581 cctttttcaa actcctgcca taaatgatga ttcaaagcct tatagctggt ttcgatttct
     2641 ggtgtcaatg tgattccttg ttctgcaaac aaagcactta aagcattggc ttcagttaat
     2701 tgaaaatcta acaatgtatc atctacatcg aacaataaag ttttatattt catctttttg
     2761 acctcctatt taaaataaat acttaacttt ttcatctctg ctaatgtcaa cgatcgatac
     2821 tcgcctactc ctagctgttc atctaattcc aatggtccca tgcttagccg tttcaatgcg
     2881 gtcactttct ttccttgggc taagaacatc tttttgactt gatggaattt tccttcttgg
     2941 atcgtcaata aaaccgtact ttggtgcttg tctgctttga gtatttttaa atcagccggc
     3001 ttacattgat acccatcagc gaatacgatt ccattttcaa atgctgttac gtctgccggt
     3061 gtcacccttt cgttcacaac tacttgataa gtctttgaaa cctttttttc tggaagtagc
     3121 atttcatatc ccaattgtcc attcgaagtg atcagcaata gtcctgttgt atctctatct
     3181 agtcttccca ctggatataa tggttggaaa cgatcggcac ttgcaatcag atcgattact
     3241 gtcgtctttt ctggatctga tacagctgta acagcttttt caggtttatt gagaagataa
     3301 taggactcat ttgtttctaa gtgttcacct gctacttcga tttgatgcag taaactatct
     3361 acatttcgat tttgtcgaaa ttcgactgtg ccatctatca tcacttgctt caaagcaaac
     3421 agccgcttca tttccttgcg tgtcgtttct aatttttctt ctattaattt atctaaacgc
     3481 atgctgtttc ctttcttatt actactaatt aattatacct tgaatattct tgcttgcaaa
     3541 tacatcatca tagttattcc ttgacttaat taacagttca cgttacggtt aaatagtaaa
     3601 gtgaattgga tttctcatac acagcgtaat gacctggtgg agaaaagttg aatcctgact
     3661 ttctaagggg agatgcgcgc atctctcctt ttgcgctttt ttaagcgccc tgccttcgat
     3721 gatgtgcacg agattagcct ttagaattca cttaaataaa atctgtccgt taccaaagca
     3781 gacagtcaca ttttaaggag gaattttggt tatgtcagta tcattagaag taagtaaaag
     3841 agaagtacgt ccccgttcat taagaaacaa acttcgtcac gaagggaaag ttccagctgt
     3901 tgtttatggg tataaagtag aaagtacccc tatctcattc gatggtttga cactaagcaa
     3961 attgttgcgt gaacatggag cgaacacagt catcacgatg gacatcgatg gacaaaaagt
     4021 caatacattg gttcacaaga tccaaacgaa cacttttacc aatcatttcg aacatgcaga
     4081 atttctatct gtaaacatgt cggaagaaac agaagtcgaa acagaagtcg ttcttgttgg
     4141 cgaatcagtg ggtgctaaat ctggtggagt gttagctcaa aacttgtata cagtagtcgt
     4201 atctgctaca ccagacaaat tgcctgaacg aattgaagtt gacgtgacaa acttggaatt
     4261 aggtgattct attacagtag ccgatcttcc aaaaaacgac gattacaaat tcgtgaccga
     4321 cagtgaagag caaatcgctg ctgtagtcgc tcaaagtgaa gaagaaagca caggtgaagg
     4381 tggcgaacca gaagtgatcg gttcaacaga agaataatac tgaaacaatc aggtggtgtt
     4441 agaagcggcg ggtcagcagc ttttaacacg ccttttttat ttctaatcgt ttattttcat
     4501 atggggcttc cacttttgta aatggcttta caaacgaaaa atcctaattt accaagcttt
     4561 tcctcgcata cttttatcgt acctttataa aaatttatac tttgaaaaaa agtattcaat
     4621 tattgttgca tttttcctga ttctttatta gaatgaaccc tcgttgaagt aagtatttct
     4681 agtttatcct aggattaatt ataaaaataa aacattcgtt gattcgaagg aggttaaaca
     4741 gatgtttgaa agttttgata gcagccgcaa ccgttacgct tcagttggtg tcgtatcaag
     4801 tttgccagat gaattgattg acagtatatg gtttattatt gacctagatc ttaagggagt
     4861 cattccatta gaaaatatat taacctttga tttgatcgaa aatcgtggaa aagtgacgat
     4921 gcatttttct caagcaggaa gcaatgtgga aatggatatt gatttgccgt ttgcctattc
     4981 gaaccaattt cctgctcaag tatacgccta tgatgacggt acgagagaaa cgatcctttt
     5041 accaagtgag attagacaat attaaggtga tagataaacg tggatgtccc ttattttaca
     5101 gaccaggcca agttgttgat tctttgactc aattcttggc ttctttttat ggagtttcat
     5161 aaattatcat tgatttctaa ttggtattct caggataaac gctatatact actaactaaa
     5221 ttttaaagat ataaggagtt taaaagatgc gcgatttaac aaaaggaagc cccgctaaac
     5281 tgattttgat gttcactgtt cctttactga tcggaaatgt ttttcagcag ttctacaata
     5341 tgatcgatat gatcatcgtg ggtcagacgc taggtaaaaa tgcgctggca gcggttggtg
     5401 caactggaag cctgacattt ttgatcattg gctttgccca aggtttaaca gctggtttag
     5461 ccattattac cgcacaacgt tacggtgcaa aagattatcg gggtctgaag aaaagttttg
     5521 ctgctagtgt tgtcattagt cttgtcgtca ctgtgatttt aactgtccta agtctcctgt
     5581 ttattcgtcc gatgcttcaa ttgatgcaaa ctcctcctga aatacttgat caagcacaaa
     5641 catttatttc aattatttta ttagggatct ttgcatcagt cagtttcaat ttgctttcta
     5701 atgtcattcg cgcacttggt gacagccgca caccactatt ctttctgatc attgcggtca
     5761 tcatcaacgt cgtacttgat ttgatcttta ttatctattt tggcatgggt gtggaaggtg
     5821 cagctattgc tacagtaatc gcacaagttt cttccagcgt actgtgtcta gtgtatatca
     5881 agaaaaaaat gcctttgtta cagttacgca aaaaagattt caaatttgaa aaagaagagt
     5941 ttgctgtaca cctaaatgct gctttgccaa tggcgtttca atcttcgatc atcgctgttg
     6001 gtgctatcgt gttacaagca gcactaaata gtctagggac agatgtcgta gctgctcaag
     6061 ccgctgctgg tcgtatcgac caatttgcaa atcagccgat gatgtcattt ggtattgcaa
     6121 tggcaacttt cactgcacaa aactatggtg cgaaagaata tggccgaatc ttgcaagggg
     6181 tacgtcaaac tttactgatg agtgtcggat ttagtctctt agcaggtgct atcgtcattt
     6241 tcttcggtca tttctttgtt caattgtttg ttagcccatc agaaacacgt gtatttgaat
     6301 tagcacaaac ctacttcaat atcaatgggt ctctttactg gatcttagca gtcctgttca
     6361 ttttacgcta cacacttcaa gggcttggac aaagcaagat ccctacgatc gccggtatga
     6421 tggaattgct tatgcgttct tttgcagcta tcgttcttac aggaatgtgg ggatacccag
     6481 gagcagcagc agcttctcct ttagcttgga tcggttctgt tgccgtgttg ctttactctt
     6541 atatgcgttc gatgaaacaa ttgaaacaca tgggtgaaga ggaacatttc acattagaac
     6601 atgcagaagc aagatagcct tgattaaaag aaatcgttgc ggctaaaaat gaaaactttt
     6661 attgaaaata tcttcttcgt ttaccgaaaa caggtttttt agataacaaa aaggctgtga
     6721 caaaaaatgt cacagccttt tttcttttat tctatttttc tttccgtgga ctctactttg
     6781 aaataatcga tatgtgcatg ctggtattct aataccgtga aacggaatga tgcaatctca
     6841 atgattgttc ctgcttttat ttctttatat cggctagaag caaagcccgc aagtgtagta
     6901 aaattcgttt cttcaaactc tggaatctct acatgaaaat aacgctggaa atcatctaat
     6961 gttagtttgc cggagatgat ataagagcca tctgactgtt ccgctatcaa tgcttcgtcc
     7021 aaatctgtct catctcggat ttccccgacg atttcttcta aaatatcttc aatcgtcact
     7081 aagccgcttg tccctccgta ttcatctttt aagatagcaa tatgctttcc cttttttcgc
     7141 atgatcgtca ataatttttt tatagggatc gtttcagttg ttacgatcgg ctcttcaatc
     7201 aactgttcta ctgtcagctg gtcatcttta taagattgct tgatcaaatc tggcaaggtg
     7261 acataaccta aaacattgtc ttttgattct aagataaccg gatagcgagt atgtccttgt
     7321 tcaatcgaag cttttatcgc ttctttgacg gtttctgaag cttcgaatac ttgcatggac
     7381 gtacggtcta cttgtatatc ttttgccaag gtatcatcaa attcaaacac gttttccaaa
     7441 taatgatatt cttctttggt caactctcct ttttcataag aattcaatgc gactaatgtc
     7501 agctcttctt gagacaatgt ctcttctcct tcagatacga gacgcacacc taatagcttc
     7561 cctagccctg cagctgaatg attcaatacc caaataaaag gaaacattac tttataaaaa
     7621 taatgaagtg gtttaactac gaacaagacg accttctccg tattgacgat actatagctc
     7681 ttaggaagca gttcgccaat aaccacatcg atatacgtaa ctaaaataaa tcccagcgcc
     7741 acagagattg tactgctaat agccgaacta aaaggtagtt taccgatcac tggttctagt
     7801 aaagccgcga cagatccttc accgacccac ccgatgatca aaccggttag cgtaattcct
     7861 aattgactag ctgataaata gttatctaaa tggtgtgtaa catgaagagc tagcttggcg
     7921 ttctttacat tttccttttc tagttgttcc aatcttgatc gtctgatttt cactaaagca
     7981 aattcacttg ctacaaaaaa tgaagtaatt gcaatcatca aaaataaaat aatgaaattg
     8041 ataaccatat tctcccgcct ttctcttttt ctattgtacc ctttttctac aaaaagattg
     8101 aaaataatgc gtattcatat ttaggaataa aaaaggagat gcggcatttt tcagccacat
     8161 ctccttatca cttcaaaatc attgcgttac ttgttgctaa acagcttttt catgacctct
     8221 gataaacggt gttcaagtag ctgacattcg acattagttc aggtcaaagc gctactttca
     8281 caaccgctct ttcaaccatg ttttccgagt atttccaatg ataagcggaa cagtttcatg
     8341 ttttacttcg ctgtactcta gcccaaacca tgtttctggc gaggtagcga tttttttgat
     8401 tgccagtttt gcctgcatga tttgacgttg ccattttcct agagttgtca cattggaaac
     8461 tacttcatca atcaggacaa attggaaatc gccaacttct cgccctggtg tcagagagta
     8521 tctttgtggc tgttttggca aacggccttg tttcatcaaa tcatggatga cttggcgaac
     8581 atatacgttg atttcctgtt ggaccctgaa acctaagaac agattcactt ggacaataaa
     8641 atcagtgtcc atcatatcta cttgatattc tttcgtatat ggctcatccg tcacttccac
     8701 atgaacaaac caatagactc gtgctctttt aggccgttta tctaaaatcg aatagatgaa
     8761 ctgtcggcca actttatctt cagccatatc aggaaccaag aagaccacat ttgtctgata
     8821 gattggtaaa gaagtatcat cacgtaattc tctaagctgc gggatataat ctctcaaaga
     8881 cacatcctcg gttgacgatt cgcgaataat gttcccttgt tcccaaataa acatcacgac
     8941 taagatgatc aaaccaatca gaacggctac aaaaccgcct cggaagaatt tgacaacact
     9001 tgagataaag aaaacgactt ctatcgcacc gaagaaaaag gtaactaaat atgcaagcca
     9061 acgagaatgc ccattttgga taagataaaa gtaaagcagt gctgttgtca tcaacatcgt
     9121 cacagtgatc gatagaccgt aagcagcttc catatgatga gacgttcgga atgtgacaac
     9181 cactagagaa caggcgatcc ataaaatcag attgactgct gggatataca tttgcccgat
     9241 actgttccca ggatacatga tctttaaccg aggcagcagt ttcaacttga ttgcttctga
     9301 aactagcgta aacgaacctg taatcaatgc ttgcgaagca atgactgcag cgactgttgc
     9361 aaaagcaacg ccgatcacga tccaactttg aggcatcatt tggaagaatg gattcaggtt
     9421 cttgatttgc tgtgcttcag ggttttgata aacattcaac aaccacgcag cctgcccaaa
     9481 ataattgata accaaacaaa ttttgacgta aggccagctc agacgaatat ttcgtttgcc
     9541 gacatgtcca agatcggaat aaagcgcctc tgcacctgtc gtagccagga aaatatttcc
     9601 aagaatgaac aagcctaatt tgttatctgc actaaaaagc agctggatag cataataagg
     9661 attcagtgca cggataactg tccagtcttg actgaagttc attaatccca ttacgcctaa
     9721 aaaagtaaac caagcgaaca tgattggtcc gaatgctttt ccgacaatat ctgtaccaaa
     9781 gcgttggacc atgaataaaa caaaaataat aactaaagtg ataatgacga tcacgttctg
     9841 gttattaccg aaccggtcaa aaaactgcgg aattcctcgc aagccttcaa tcgcagtcgt
     9901 taccgtcaca gcaggggtca ataccccatc tgctagtaag gcagctcctc cgatcattgc
     9961 aggaatgatc aaatatttcc caccttttcg gacaagtgtg tagagtgaga aaattccacc
    10021 ttcaccatgg ttatctgcat tcaatgcaat cacgacatat ttgattgttg tcagtagagt
    10081 caatgtccaa aaaatcaatg agactgatcc gataacaaaa tcaggattga ttcctgacaa
    10141 tccgccatta tcttcaacaa tcgatttcat aacgtaaagc ggacttgtcc cgatgtctcc
    10201 gtagacgact cccatcgcta caagcaggcc agccattgat atctttttgg tattgtgcgt
    10261 tgttgccacg tttttccctc atttcttgtt atatggatat cctttaggta agcttgaatg
    10321 atagatgata ttcttatcca tgacacccgc tttcctaaat tgcttctaat tatccaagta
    10381 acacactgaa gtgtcaactt atttgaataa aattattctt tctttggacg ttctctgata
    10441 cgtcttgcca caaatgaata aagagcaagg caaaaaattg cacccaataa tgctggtatc
    10501 aaataaaaac cgccgagttc cggtcccagt ggtcctagta aaaattcgcc gagccaacta
    10561 cctaaaaaac cgataatgat atttccagat acgcctcccg ggacttcctt tcctaaaatt
    10621 gcgccagcta tcaaacctaa tataccgccg ataaataacg accataaata attcagcatc
    10681 tcctttccct cctttatcca tatttctcac ttttatttta cgctttattc agaaggactc
    10741 caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg taacaaaagt gttcagctta
    10801 tttccgtaga agctgcttct ggaacaccgt gaatcagagg ttgtgaaatc agcggtttga
    10861 cctgagtatt agagaaacaa acggaaaaat agttcctcat attttgccgt tttttgagct
    10921 aatatcgagg gacagctgat tgaacatcgt ttattggaga ggttgtaaaa aagctgttct
    10981 gcatcaagta ataagaacag ccaagtaaaa acagctccta atgtttttaa cttggctgga
    11041 cttattaccg cagatgcaag cttttgaaca ccgtttattc gaaaaaaaag gactgtgaca
    11101 cggcttttgt cacagtcccc ttttttcgaa taattattgt ccccctgtca ccttcacgat
    11161 cacaagtaca ttattcctat gaattaaatt atttttcttc atttttcgtt gaaccatctt
    11221 cgttcaacat tgcttttctt gacagattta cgcgtccttg tttatcgatt tcagttactt
    11281 tcacaaggat ctcatcacct agtttaacaa catcttcgac tttattgaca cgatcgtttg
    11341 ctaattggga aatatggaca agtccatctt ttcctttgat caagttgacg aatgcaccga
    11401 atttttcgat acggacaact ttccctagat aaacttcacc aactttgact tctttggtca
    11461 aatcttcgat gatcttgata gcttttttga tcatttctgc atcagctgaa gcaatactta
    11521 cttttccatc ttgatcgata tcgattttca caccagtttc atcgatgatt ccgttgatcg
    11581 tatcgccgcc tttaccgata acatctttga ttttagcagg ttcgatctgg atcatttcga
    11641 tttttggtgc atattggctt aattcttcac gtggagcagc aatcgttgaa gtcaattctt
    11701 ctaagatttc catacgtgct tgttttgctt gagttaatgc ttcagttagg atttgttctg
    11761 tgattccttg aatcttgata tccatctgta aagcagtgat accatctttt gtaccggcaa
    11821 ctttaaagtc catatcgcca agatgatctt ccagcccttg gatatcagtt aggatcgtat
    11881 agttttcacc atcagaaaca agtcccatcg caataccggc gactggcgct ttgattggta
    11941 cacctgcatc catcaatgct aatgtgccag cacagatact tgcttgggaa gaagaaccat
    12001 ttgattccaa tacttctgct actaaacgga tcgtataagg aaattcttct tctgaaggga
    12061 ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc gatttcccga cgccctggag
    12121 agcctgcacg tcctgtagaa ccaacagaaa attgagggaa attgtagtgg tggatgaatc
    12181 gtttgctgac ttccacccct aaaccatcaa tgatttggtg ctctcctaga ggtgcgagtg
    12241 tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa acctgatcca tgtacacgtg
    12301 gcaataagct gacctctgat gacaatggac ggatttcatc caatttacgc ccgtcaggac
    12361 ggatcttatc gatggtgatc aattcacgta cgacatcttt ttcaagatct tctgtgattt
    12421 gtttaacttc tttcaacaag tgatcttcat cttcatgacc tgcaaatttc tcagcataag
    12481 cttcgcggat atcgatcttc acctgctcga tattatcttc gcgagcaagt ttttcttcag
    12541 tcattactgc tgctttcatt gtagcgtaag aagcatcaaa gatttctttt ttcaatactt
    12601 catcaacttg cagtaaagtc acttccattt tttctttccc tactgcttga acgatttctt
    12661 cttggaaagc aactaactct ttgatagcgt caaaaccaaa cagcaatgca ccaagcatgt
    12721 cttcttctga aacttctttc gcgccgcttt caaccatatt gatggcttgt ttcgttccgg
    12781 caacagtcaa ttcgatatct gtttgttctg cttgttctac tgttggattc aagacgtact
    12841 cgccatttac acgaccaact tctacgcctg cgattgggcc gtcaaatggg atatctgaga
    12901 ttgatagtgc taaagaagaa cctagcattg ctgccatagc tggcgagcag tctgtttcta
    12961 cactcatcac gatgttcgtt acttggactt cattacggaa gccttctgca aacatcgggc
    13021 gaattggacg gtcgatcaaa cgggcagtca atgttgcttc cgtacttgga cggccttctc
    13081 gtttgatgaa acctccaggg attttccctg ctgcatacat tttttcttca taattgatcg
    13141 ttaatgggaa aaagtctgta tcttttgctt ctttagaggc cacagctgca cttaagacta
    13201 ctgtatctcc ataacggact aaaacggctc cgtttgcttg ttttgctagc tgaccgactt
    13261 cgatttgtaa gggacggccg ccccatgtcg ttttgaatac ttgtttttct gacataactt
    13321 ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa atgaaaactg ccgaaagccg
    13381 tcttttgagc agctttcatt tggttagtag gagtccatct agcttgatca cggaatttta
    13441 cttaattgat atacgagtaa aaaaagtgag attcatgtga atcccactct ttttgactaa
    13501 aattaacgac gcaagcctaa acgtttgatc aattcgcggt aacgttgaac gtcagtttta
    13561 cgtaagtaag ctaacaagtt acggcggtga ccaacttttt tcattaggcc gcggtatgaa
    13621 tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt ggttgatttc ttcagttaat
    13681 acagcgattt gtacttctgg agaaccagta tccccttcat ggcgagcgta ttcgttgatg
    13741 atttcattct tgcgttcttt tgaaattgcc atttctttca cctctttcga ttattcccct
    13801 gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg caggtcatgt gcctagagca
    13861 cacagctata ctttacctga aaccgggttg aaaatcaagt agttaatcat taagaaaagg
    13921 tagttttcaa acaatcttac tactactttt ccatttttgt ggtaaactat aatgcgaaaa
    13981 tagacttgaa acacagtctt ttaagaaagg ggtacatatt ccatgattac aatggatgat
    14041 atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa aagaagtttc tcttccatta
    14101 agcgaagaag atatctcttt aggaaaagag atgcttgagt tcttaaaaaa cagccaagat
    14161 ccaatcaaag ctgaagaact acacttacga ggcggtgttg gcttagctgc tcctcaatta
    14221 gatatttcta aaagaatcat tgctgttcac gtaccaagtc ctgacccaga ggcagatgga
    14281 ccttctctaa gtactgttat gtataatcct aaaattttga gccattctgt tcaagatgct
    14341 tgtttagggg aaggtgaagg ctgtttatct gttgatcgag aagtaccagg atatgttgtt
    14401 cgccatgcca aaatcactgt gtcttattat gatatgaatg gtgaaaaaca caagattcgt
    14461 ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg accatatcaa cggtgtgatg
    14521 ttctatgacc acatcaacga tcaaaatcca tttgcactaa aagaaggcgt attggtgatc
    14581 gagtagagaa ttggcaggtt ccttcgatac tatgctacaa gaaagcttta taaaaaaggg
    14641 gagtgctcaa aagctgcact tccctttttt atgtttctcc actaatcgaa atttagatag
    14701 acatctagaa atcgcaattc caaaaaattc tgtgtattgg atacgcatct tttcttattt
    14761 tttcttattt cttccatatg ggtgtttatc tcagagatga tagaatagtt tctaggataa
    14821 aaagtagcag ctacgatgtt atccttcgta gaggattcct gcttcagcta gtttttgttt
    14881 gatgtttttc agcatatgct tttctgggta gcggatcgta tgcagatgga tgccgcctgt
    14941 taattcggat aacaaagtag cattactttt ttgataacgt ctcataaagg aatcgatatc
    15001 ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg taaaggggat gttccacaat
    15061 tacatcaatg atttcaccac cattttcaac gattagtctc agttcttctt ctgtttgatc
    15121 ttttccatgc tggacagcga ttttactgat gtgcccagta ttggtttctt ctgtttcgag
    15181 gatataacct cttgctgttg ccacgatact tttacctgca gctcgtagta aagcaatatc
    15241 gcctacaatg atctgacggc tgactccaaa agttttcgca aacttgctag cactcactgg
    15301 tttttctgta tttgccaagg tttgcatgat ttgttttcgc cgtgtctctc cttcgatcac
    15361 acttattcct ccttcatttt gttttggtat tcgcgcgagt gtttcatcaa atcaacatac
    15421 gtgtctgtta ttgtttcctt atatccctta tttttcagca gactaacaac tttatccaat
    15481 acttgctgct ctctttttgc atcataaact gcttggtttt cttctttttt cacttgtcct
    15541 attttagtta ccgtatccat tcttttttct aataaagcaa ccaactctcg atcgatcata
    15601 tcgatcttat gacgtaatgt ttcaagagac tcttctgtta gttcttcctc tactgtcttg
    15661 atgatacgag caggatttcc tgcaatcact acattgtcgg gaaaagattt ggtcacaacc
    15721 gaacctgcgc cgactactac gttgtcccct agagtgacac ccggcgtgac gataactcct
    15781 ccgcctaacc aaacattatt accgatttta atgggttttg cgtactccag accgctgtta
    15841 cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct ggacattcgg agcgaacatg
    15901 cagttatcac cgatttcaat cgtgctgaca tctaaaaatg tacaattgaa atttgcataa
    15961 aaattctccc caacaaatat attatatccg taatcaaaat tgataactgg ctccatatag
    16021 atttttttcc ctgtacggcc aaaagtctct ttcaacaatt gggatcgcag ttccgctgat
    16081 tctgcttggt tgatgatttg gctttgactt cttgcaaaca tacggtcggc aaccagctca
    16141 ggatcattgg caaaataaag ctcgcctgca atcatttttt ctttttcggt tttgaattca
    16201 gtcataatct gctcctaatt cttgtatttt tttgaaaaca accgctccgt cttcttgaat
    16261 aaacgtataa ggctccgcta aataaacagg cgtttgattg attactaaga ttttggctga
    16321 cggctggcga aactgtacca gatcacaaaa cggatgaact tgaaaactcg ttccaacaat
    16381 gacgatcagc tctgcttggc tgacggcata agccgcctgc tccatgactc catcttgaaa
    16441 cccttcacca tataacacga tgtctgggcg tatttgtcct ccgcatgctt gatgtttgtc
    16501 gctgtgaaga tattctttcc agtctaccgt ttgttgacat tttcgacaat gacattgata
    16561 cagactccca tgaaaattga cgagttgcgg gcttttagcc tttgcgtgca aaccgtcgat
    16621 gttttgggaa acaacccaaa catttttttc tttcgctaga tgtgctattt ccaaatgaat
    16681 gatattgggt tttgcttcag gatgataaat ctttttgaca aattgataga atttatccgg
    16741 ttcattcacc attgcttgat gactcagcaa gtattccggc tgctccatcc cgtggtagat
    16801 tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg gatacccctg caccggtcaa
    16861 aaatgtaatt tgctgataag ctaatatctc ttttgctgct tcgtctactg ttaattgttt
    16921 catgttttgt cacctcactt tccattatag cccatactcg aacaaattta ttgttgaaaa
    16981 gtcagaaaaa tgggaataaa aaagtgttat gggcatttga gcaatttatc acaatgttca
    17041 ctgacaaaag aatgaaaacg tgttacaata ctgctgtata aaagtgaggt gaatcgatat
    17101 gttaccattc tttatttatt ggggcgttat tctggcttgt atcgcctggt tagcgttaag
    17161 cgtttatttc tctgtttttt atcttgtacg caaagaaaat ggaaatcttt gggcttttgc
    17221 tttattcaac gtgattgccg caattgtttt agccattaca ttagccgtat atcgaacatg
    17281 gggctgggga atcacccaat actcaagttt gatttatcta atattagcca tttatggcgt
    17341 cgttgttctt ttgcaagcga tcctaggacg tgagccaaag aaagcggcag cttaaatgat
    17401 cgagatggaa ctggacacag gaaattttct gttctagttc tttttttatt gtattacata
    17461 catttttgtg tataattata ctatcgtttg caatggatag atgattcaca ttttgattgt
    17521 tttttcggta aaagtaaaga tttctatatt tatcagtatt ttttcaaaaa ccagcagtta
    17581 tagattgaca aagcctctcg agtatcgtaa actttttttg tataaataac tgaatacgag
    17641 atgcctcaat caatgaggta gaggtcgcga gaatcattaa ccctgtggag tgaagcaaac
    17701 atggagaagc aggaataggg aggatcgccg aaatgtgcac ttttttgctt ggagtgtatg
    17761 ttgggacgca agttaagagc ttgcggactg tcttagcagt gatgctaagt tgcgctatgt
    17821 tttgtgagag gtttatcacc ttatggaatt ttagcggcct ttttatatct ataaatcggt
    17881 cgctttttat tttttcagat aaagctttac cgcatttcat ctagaccgct cgtgtatcat
    17941 ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt tcagttgccg tactatttgc
    18001 ttttgtcttc tcactattca gtgtgggatc tctggcaaat gcagatgaaa aaacaccgag
    18061 cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc ttcaactggt ctcaacaaac
    18121 agatgagaat aatgccttgc caattcaagg ccaaaatagt tacgccggcg gttatgatgt
    18181 tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa ttagtcatcg tccaaacgaa
    18241 atgggatggt ctggctcctg cccttcaatc aggcaagatc gacgcgatca tcgccggaat
    18301 gagccctact gctgaacgac gaaaagaaat tgattttacc gatccttatt acgaatcaca
    18361 attagtagta gtagttcaaa aagatagcaa atatgcaaaa gcaaaaagtt tagcagatct
    18421 ttctggagca aagatcactg ctcagttaaa tacattccac tatactgtga tcgatcagat
    18481 accagatgtg cagaaacaac aagcaatgga taatttctca gcaatgagaa cagctttagc
    18541 ttctggaatg atcgatggtt atgtcagcga acgtccggaa ggtgtaacgg caactagtgt
    18601 gaacaaagac ttggaaatgc ttgaattcag cggtgcagat ggcttccaga caaacccgga
    18661 agatgttcag atttctgttg gtatgcgtaa aggcgacccg gatatccaaa aagtaaacca
    18721 gattctttct ggcatctcta acgatgaacg aaccaaaatt atggaccaag cagtcaaaga
    18781 tcagcctgct gcaactgata gtgaagaaaa acctggcatc atttctgatt tcactacgat
    18841 ttggaaacaa tacggcaata tgttcttacg aggtgctgga cttaccttgt tcatcgcatt
    18901 gatcgggaca gttgtcggta ctacacttgg attactgatt ggtgtcttcc gttcggtacc
    18961 tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc ggaaatgcac ttctttctat
    19021 ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa gcaatggtca tcttctacgg
    19081 tctggcactc gcttttggca tttcattgaa ccgaacgatt gccgctttgt ttattgtctc
    19141 tctcaatact ggagcttaca tgacagagat cgttcgtggg gggatttttg cagtagatga
    19201 agggcagttc gaagctgcgc aagccattgg tatgacccat ggccaaacga tgagaaaagt
    19261 tgttattcca caagttttac ggaatatctt gccagcaaca ggaaatgagt ttgttatcaa
    19321 tatcaaagat acagcagttt taagtgtaat cggcgtagct gatttgttct tccaaggaaa
    19381 ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt acgatcgtcg ggatcatcta
    19441 cttagtcatg acctttacga tcaccagaat cctgcgggcg gtagaaaaga aaatcgatgg
    19501 tccttcagct tataccaagc ttgaagaaat cgacaattca aatttgcagg aataaggagg
    19561 aagaatcatg aatgaaaaaa ttattgaaat caaacatcta agaaaaagct tcggtgaaaa
    19621 tgaagtattg aaagatatca gcgtaacagt caataaaggt gaagttgtga cgatcatcgg
    19681 ttcttccggt tctggtaaat ctactttctt gcgctgtatc aatttactgg aaaaaccaac
    19741 aggcggcgag atcatttata atggagaaaa tgtattagct cctaaatata atttacctaa
    19801 atatcgaacg aatctaggta tggttttcca atccttcaac ctgttcaaca atatgaatgt
    19861 cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa cgagataaag aaactgccaa
    19921 aaaagtagca atcgaaaatc tggaaaaagt tggtatggat cgttatatcg aagccaaacc
    19981 agcacaatta tctggtggac agaaacagcg tgtcgcaatt gctcgggcat tatcaatgaa
    20041 tccagatgta ttgctattcg acgaacctac ttcggcgtta gaccctgaaa tggttggcga
    20101 agtgcttaat acgatcaaag acttagcaca tactggcttg acaatgctta tcgtgacgca
    20161 tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt tttatggata aaggtgtgat
    20221 tgcagaagaa ggtacaccag aagatatctt tgtccatccg aaagaagaac gtacaaaaga
    20281 attcctttca cgtattttaa atgcttagga tcaatagctg attgggacaa agagctgcgg
    20341 tgtttaccgc agttcttttt tatgaattac tcataaaagg taaatttctg aaaattttgg
    20401 aatcttagt
//
LOCUS       DAGCWU010000037        20363 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.037, whole genome shotgun sequence.
ACCESSION   DAGCWU010000037 DAGCWU010000000
VERSION     DAGCWU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.037"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            292..1431
                     /locus_tag="MBZ71_001059"
     CDS             292..1431
                     /locus_tag="MBZ71_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBT4429030.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="MBZ71_001060"
     CDS             complement(1498..2073)
                     /locus_tag="MBZ71_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4429031.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="MBZ71_001061"
     CDS             complement(2078..3052)
                     /locus_tag="MBZ71_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBT4429032.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="MBZ71_001062"
     CDS             3335..4303
                     /locus_tag="MBZ71_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4429033.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="MBZ71_001063"
     CDS             4416..4694
                     /locus_tag="MBZ71_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBT4429034.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="MBZ71_001064"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="MBZ71_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBT4429035.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="MBZ71_001065"
     CDS             5151..5906
                     /locus_tag="MBZ71_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4429036.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="MBZ71_001066"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="MBZ71_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBT4429037.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="MBZ71_001067"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="MBZ71_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBT4429038.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="MBZ71_001068"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="MBZ71_001068"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBT4429039.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="MBZ71_001069"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="MBZ71_001069"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBT4429040.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="MBZ71_001070"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="MBZ71_001070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBT4429041.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="MBZ71_001071"
     CDS             10643..11185
                     /locus_tag="MBZ71_001071"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBT4429042.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="MBZ71_001072"
     CDS             11208..12764
                     /locus_tag="MBZ71_001072"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBT4429043.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="MBZ71_001073"
     CDS             12778..13680
                     /locus_tag="MBZ71_001073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBT4429044.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="MBZ71_001074"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="MBZ71_001074"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBT4429045.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     RGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="MBZ71_001075"
     CDS             15123..15545
                     /locus_tag="MBZ71_001075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBT4429046.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="MBZ71_001076"
     CDS             16014..16244
                     /locus_tag="MBZ71_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBT4429047.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="MBZ71_001077"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="MBZ71_001077"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBT4429048.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="MBZ71_001078"
     CDS             17655..17831
                     /locus_tag="MBZ71_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBT4429049.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="MBZ71_001079"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="MBZ71_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBT4429050.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="MBZ71_001080"
     CDS             18326..18709
                     /locus_tag="MBZ71_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4429051.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="MBZ71_001081"
     CDS             18878..19762
                     /locus_tag="MBZ71_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4429052.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="MBZ71_001082"
     CDS             19876..20112
                     /locus_tag="MBZ71_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBT4429053.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="MBZ71_001083"
     tRNA            20200..20280
                     /locus_tag="MBZ71_001083"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="MBZ71_001084"
     tRNA            20287..20358
                     /locus_tag="MBZ71_001084"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3567 c         4426 g         5611 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacgaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta tac
//
LOCUS       DAGCWU010000038        20176 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.038, whole genome shotgun sequence.
ACCESSION   DAGCWU010000038 DAGCWU010000000
VERSION     DAGCWU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.038"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            105..962
                     /locus_tag="MBZ71_001085"
     CDS             105..962
                     /locus_tag="MBZ71_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBT4429054.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1077..1997
                     /gene="pstC"
                     /locus_tag="MBZ71_001086"
     CDS             1077..1997
                     /gene="pstC"
                     /locus_tag="MBZ71_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBT4429055.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1997..2881
                     /gene="pstA"
                     /locus_tag="MBZ71_001087"
     CDS             1997..2881
                     /gene="pstA"
                     /locus_tag="MBZ71_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBT4429056.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2896..3702
                     /gene="pstB"
                     /locus_tag="MBZ71_001088"
     CDS             2896..3702
                     /gene="pstB"
                     /locus_tag="MBZ71_001088"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBT4429057.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3718..4476
                     /locus_tag="MBZ71_001089"
     CDS             3718..4476
                     /locus_tag="MBZ71_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBT4429058.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4489..5166
                     /gene="phoU"
                     /locus_tag="MBZ71_001090"
     CDS             4489..5166
                     /gene="phoU"
                     /locus_tag="MBZ71_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBT4429059.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5404..6435
                     /locus_tag="MBZ71_001091"
     CDS             5404..6435
                     /locus_tag="MBZ71_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBT4429060.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6432..7199
                     /locus_tag="MBZ71_001092"
     CDS             6432..7199
                     /locus_tag="MBZ71_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBT4429061.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7299..8867
                     /gene="liaX"
                     /locus_tag="MBZ71_001093"
     CDS             7299..8867
                     /gene="liaX"
                     /locus_tag="MBZ71_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBT4429062.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9164..9484
                     /locus_tag="MBZ71_001094"
     CDS             9164..9484
                     /locus_tag="MBZ71_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBT4429063.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9484..9834
                     /locus_tag="MBZ71_001095"
     CDS             9484..9834
                     /locus_tag="MBZ71_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBT4429064.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10001..10939
                     /locus_tag="MBZ71_001096"
     CDS             10001..10939
                     /locus_tag="MBZ71_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBT4429065.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10954..11784
                     /locus_tag="MBZ71_001097"
     CDS             10954..11784
                     /locus_tag="MBZ71_001097"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBT4429066.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11828..12853
                     /locus_tag="MBZ71_001098"
     CDS             11828..12853
                     /locus_tag="MBZ71_001098"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBT4429067.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12868..13806
                     /gene="galU"
                     /locus_tag="MBZ71_001099"
     CDS             12868..13806
                     /gene="galU"
                     /locus_tag="MBZ71_001099"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBT4429068.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13931..14371
                     /locus_tag="MBZ71_001100"
     CDS             13931..14371
                     /locus_tag="MBZ71_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBT4429069.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14374..14976
                     /locus_tag="MBZ71_001101"
     CDS             14374..14976
                     /locus_tag="MBZ71_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4429070.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15035..16141)
                     /locus_tag="MBZ71_001102"
     CDS             complement(15035..16141)
                     /locus_tag="MBZ71_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBT4429071.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16351..17352
                     /gene="ccpA"
                     /locus_tag="MBZ71_001103"
     CDS             16351..17352
                     /gene="ccpA"
                     /locus_tag="MBZ71_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBT4429072.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17689..20034)
                     /locus_tag="MBZ71_001104"
     CDS             complement(17689..20034)
                     /locus_tag="MBZ71_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBT4429073.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT         6416 a         3566 c         4289 g         5905 t
ORIGIN      
        1 tacaattatg agaatttatt ttgttattta atgattttga ttcgttttca tcaagtaatg
       61 aaaatgataa aataaacata ctttaggatt aaaggagatt tctaatgaaa aaacttaaat
      121 tattcttacc aataatagga ttagctattc ttttatcagg ctgttcaaag tggattgatc
      181 gaggagaatc cattacggct gttggctctt ctgctttaca gcctttagta gaaacagttg
      241 ctgaaactta ccagacagaa aatcctggaa aattcatcaa tgtacaaggg ggcggaagcg
      301 gaactggttt gtctcaagta cagtcaggag cagtcgacat cggaaactct gatttgtttg
      361 cggaagaaaa atcggggatc gatgcctcag cattagtcga tcatcgagta gcagttgttg
      421 ggatcactcc tattgtgaac aaggatattg gcgtaaaaga catcagtatg gaaaacctga
      481 aaaaactatt tacaggcgag atcaaaaact ggagtgaagt cggtggaaaa gatcagccag
      541 tagttatatt gaaccgcgct tcaggaagcg gaacccgtag cacgtttgaa aaatgggtat
      601 tggacggaga aacagctgtt caggcacaag aacaagattc aagcggaatg gtgcgccaga
      661 tcgttgccga cacacccggt gcaatcagct atgttgcttt ttcttatgtt tccgatgatg
      721 tagatacgtt gaatgttaac ggtgtagaac cgacagatga aaatgtgaca acgaacaaat
      781 ggacgatttg ggcatatgag catatgtaca caagagatca tccaaaaaaa ctgacaaaag
      841 aatttttaga ctatatctta tctgatgaag tacaagataa catcgttgga gaactaggtt
      901 atatccctgt ttctcatatg aaagtcgaac gtgattggca aggaaatatc gttaaagagt
      961 aatctttaca caaaatttac aaacttagct aaacttttac atgttattaa aaccaaattg
     1021 atgttcaatt tgtacactga gtgagtatgt taaacctgat taagggggag tcatctgtgg
     1081 aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc aaaaatggaa cagcgtggaa
     1141 aattcatcag ttttttatgt atcgcgctga tcgtgttggt tgtattatct attttttatt
     1201 ttgtagctag taaaggattg gccacctttt ttactgataa gatcaatgta tttgattttc
     1261 tgtttggtac agattggaat ccaagccaaa tcggagcaaa tgggaaacca atggtagggg
     1321 cattgcctat gatcacaggt tcgtttattg tgaccttttt atctgctatc gttgccacgc
     1381 catttgccat tggtgcagca gtattcatga cggagatctc tcctaaaaac ggaacgaaga
     1441 tcttacagcc ggtcatcgag ctgcttgtag gtattccttc tgtcgtttat ggttttatcg
     1501 gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg cgggactggg tttggtatct
     1561 tagctggtac atttgtgttg ttcgttatga tcttgccgac tgtcacaacg atgactgttg
     1621 atgcattaaa ggcagtgcca cggcactatc gggaagcttc attagcgctt ggtgccacaa
     1681 gatggcaaac aatctacaaa gtcgtgctac gagcagccgt gcccgggatt ttaacagcag
     1741 tcgtttttgg tatggctcgt gcgtttggtg aagccttagc tatccaaatg gtcatcggta
     1801 atgcagcatt gatgccaaca ggtcttacta ctcctgcttc tacattgacg tccatcttaa
     1861 caatgggaat cggaaataca atcatgggaa cagttgaaaa taatgtgtta tggtcattag
     1921 cgctgatctt gctattgatg tcattattct ttaatatcgt gatccgtatg attgggaaga
     1981 aaggagagct gaaataatga acgcaaaacg ctcagataaa attgctacag gtgttttata
     2041 tatagtttcg gggatcatcg tattgatttt agcagcttta ctactttata tcttagttcg
     2101 gggaataccg cacatttcat ggtccttctt gaccgaacct tccaaagcct accaagaagg
     2161 cggtggaatc gggatccagc tatttaactc gatttatttg cttttgatca cgatgatcat
     2221 cagttttcct atttctttag gtgcgggtat ctatctttca gaatatgctg gaaaaaactg
     2281 gctaacagat gtgattcgca cgtcgattga aatcttgagc tcgttgcctt ctgtcgttgt
     2341 cggtttattc ggtttcttgg ttttcgttat tcaattccag tatggtttct cgattatttc
     2401 tggtgcattg gctttgactg tattcaactt gcctcttttg actagaacag ttgaggagtc
     2461 tttacaagcg gtccattaca cacaacgaga agcaggattg gccttaggtt tgtcacgctg
     2521 ggaaacagtg ataaaagtgg tggttccaga agcgacacca gggattttga ctggtgtgat
     2581 cctaagttct ggaagaattt ttggtgaagc agctgcattg atctatacgg ctggacaaag
     2641 tgcacctgcg ttagatttta cgaactggaa tccattaagt gtttcaagcc cgatcagcat
     2701 tttccgtcaa gctgaaacat tagctgtcca tatttggaag atcaatacag aaggcacgat
     2761 gcctgatggt gtttctgttt cggctggcgc ttctgccgtg ttgattattg ctgttttact
     2821 gttcaacttc ggcgctcgtg cgattggcaa acgattatac cgaaaaatga cttcagccta
     2881 aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa caaatgtcat caccatgcca
     2941 aaagagaaag aagtagcact atatacggat gatctacatg tctggtatgg cgacaatgaa
     3001 gcaatcaaag gagtggatct ggaatttgaa aaaaataaga tcactgcact gattggtcct
     3061 tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc gaatgaatga tgggatcgct
     3121 aatacgaaaa tcactgggaa aatcatgtat caaggcgtag atattaatgc gcctcaagtg
     3181 gatgtttatg agatgcgcaa acggattggc atggtttttc aacgtcctaa tccgttcagc
     3241 aagtcgattt atgacaatat tacatttgct ttgaaacagc acggcgaaaa aaacaaaaag
     3301 aaattagatg aaatcgttga aacaagcttg aaacaagctg ccttgtggga ccaagtaaaa
     3361 gacagcttgg acaaaagtgc cttggcttta tctggcgggc aacagcaacg tttatgtatt
     3421 gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag acgagccggc aagtgctttg
     3481 gaccctattt cgacaggaac ggttgaagaa acacttgtaa acttgaaaga agactatacg
     3541 atcatcatcg tgacgcataa catgcaacaa gcagctcgta tcagcgacta tactgccttt
     3601 ttctatttag gaaaagtaat ggaatatgac aaaactaaca agatatttac cagaccaaaa
     3661 attcaggcaa ccgaagatta tgtgtccggt cattttggtt aagacggagg cctcaacatg
     3721 acaaaagaga ttatttcatc taaagatctt catctgtatt atggaaagaa agaagcacta
     3781 aaagggatcg atctaagctt caatcaaggg gaaatcactg cgatgatcgg tccttctggt
     3841 tgcggaaaat ccacttatct gcgttctttg aaccggatga atgacctgat tccgaatgta
     3901 acgatcaccg gaagtgtttt atataaagac aaagacatct atggtccaaa aacagatgtt
     3961 gttgaactaa gaaaagaaat cggcatggta ttccagcagc caaacccatt tcctttttct
     4021 atctatgaaa atgtcatcta tggattgaaa ttaaaagggg aaaaagataa aaaagtcctt
     4081 gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt gggatgatgt gaaagacaaa
     4141 ttgcataaaa gtgctttatc tttatccggt gggcagcagc agcgtgtatg tatcgcacgt
     4201 gttttggcag ttaatccgga aatcatctta ttggatgaac caacaagtgc gcttgatcct
     4261 gtttctactg ggaaaatcga aagtatgctg ttagaattaa gagagcggta tacgatgatc
     4321 atcgtgaccc ataatatgtc tcaagcttca cgtatttcag ataagacggc attcttttta
     4381 gatggtcact tgatcgaata caatgatacg aagaagatat tcatgaatcc agaaaaacaa
     4441 gagacagaag attatatttc aggtcgattt ggataaggag gaaacggaat gctacgtaca
     4501 caatttgaag aagaattgct gaatctccat aatcagttct acgaaatggg gatgatggtg
     4561 agcagcgccg tgcacaagtc agttcgtgca tacatcaaac acgataaaat cttagcccaa
     4621 gaagttatcg aaaacgatat aaatatcaat aacatggaaa cacgcttgga gaagaaaagt
     4681 tttgaaatga tcgcattgca acagccagtt acaactgatt tgcgtatgat cattacggtc
     4741 atgaaagcta gctcggattt agaacgtatg gccgatcatg cggtgtctgt tgctaaatcc
     4801 acgattcgcg taaaagggca gacgagaata ccggaaatcg aaaaagagat ctccgatatg
     4861 tccgattatg tgaaaaaaat ggtggacaac gtcctagttg cttatgtcaa aacagacgaa
     4921 aaagacgcac gtacgatcgc caatatggat caaagagtca atgactattt caatcgaatc
     4981 tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga cagtcatcag tggaacagac
     5041 tacttgaatg ttgccagtta tctagaacgg atcggagact atgtgacaaa catctgtgaa
     5101 tggatcgtct acttggctac agggaaaatc actgagttaa atacgaacca caatgagatt
     5161 ttctaaactg aagctgtgat tatgccgttt gtttaggaaa agaggttgtg aaatcagcgg
     5221 ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc aaacaaaaaa tagctcctca
     5281 tatttttcgt ttggctggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     5341 ggaaagggtt tgtgacaaaa catgtcacaa acccttttca tcatttcata gaaaggataa
     5401 agaatgcaaa aaaaaattag catcatcact atatttttta tttgtgtgct tacttcatgg
     5461 gttattcagc atcaagccgc tttatatgaa gtacctgtca tcgagataga aaaagtacga
     5521 ataaaagagg atcatcaatt gattacagga aaactgatga acacggatca aaaaggagaa
     5581 tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag aaacagcaaa tataaaagcg
     5641 agacaaatgt atctagtcca aatgcatgga gatgaatggc agctagtagc aaaaaaaaat
     5701 gatggctggc ttttcttctt cacaagtatc ttcattggcg ccatgttgct tattggtgga
     5761 aaacagggtc tatttgcttt aggaagtctc atcatcaata tcgttttact tttacttttg
     5821 ttgctcatct ttactaaaac agaagggatt tctctattaa atatatctat cctgttcgtc
     5881 gttataggga tgattattag catattggct ttagatggat ggaatagaag cagtataatc
     5941 aaaatttcag ctgcagttac gagtgttttt ttagcatttt ttatttgtct tttgacaatg
     6001 aatcattgga aagaccaagg actgcgctat gaggaactga attatctgac acgaccgtat
     6061 cgttctgttt ttctttctag cgtactgatt ggaacagctg gaggaacact cgataccgtt
     6121 atcacagtca ttgcgacatt agaagaattg actaagcgac aaccagaaat aacagcgaaa
     6181 cagttatggc tttcaggaag gcaagtgggt cgagatgtga ttggctcgat gaccaatatc
     6241 cttttgttcg tctatatcag tgggtcagtt ccatcgcttc ttttatattt aggaaatcaa
     6301 tggtcgttca aagaaacgat agaacagcat ttatcattgg aatttttacg cgtcattgct
     6361 ggcagcttag gaatagtatt atcgatccct atctcggtcc tcttttttct gactgtaagg
     6421 aggctgaaac gatgaatgta cttattatac ttgttgggat tttagcaatc agcgtgctac
     6481 ttattggcgg tacgcaaggt atgtatattc ttttaggctt attcatcaat ttagggatct
     6541 ttttcctgtt gttattcggt tatcatcaga agtggccgat actggcattg tcggtcatag
     6601 gatttttatt gattgctgtg gttatcttat tttttatcaa cggttataat ctaaaaatgc
     6661 gtgcagcttt tgtgagtata ctgatctttt tattttgttt cctactattg cttcctatca
     6721 ctgatttttt agcaatccaa ggttttacat cggtagaact agaagaatta tctggcttag
     6781 ataagacttt agcgattgat tttagattac ttacgagatc gttgcttctg attagtctta
     6841 gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg gacattcgaa gtatatcagg
     6901 caaatccgca attatctttt taccaattgc gccattctag tttcgctatt gctaaaaaaa
     6961 tattggcgtc aacagtaatg acacttcttt ttgcattcat gggaagttct ttagcgttga
     7021 tcctctggtt tattgattta gacattcctt ttcagcaagt gatcaatgaa aaatcatttg
     7081 ttctagaata tacgatggct attcttacca cattgtccgc actacttgtt cttcctttaa
     7141 cctgcgtgac tacagcatat ctctttacaa aaaagaaaga acaactcaag atggagtaaa
     7201 agttacaact taagtcctat gacattttta ctgaatcagt gcataatatg gatgtaagga
     7261 aaagcttgca aacaattttg cttaaggagg caatttccat gaaagaaaga gaacgtattt
     7321 tagatttagt gaaaaaagga atcctttcga ctgaagaagg cttagattta ttagaaagca
     7381 tggcaactga aaaagacgaa aagcaaatca aaaaagaagc agacaaggta actgctagtc
     7441 ataaagaaaa agatcaggca agtcaattga tcgataaact tgaaaacggc gaagaagaaa
     7501 tttctgagcc agttgatcca aaggaaagag aaagacaaga tcaagaaaac ttagaaaaga
     7561 tcttagatga actagcaact gaagcgaata aaacttcggc gcgtctagat gaagtaaatg
     7621 ctcaattcgc tgacaataag gcagcacgca atgagaaaca ggaagcattg atgcagctga
     7681 atacaaaaga agaattaggc gagttgacag aagaagaatt agcacagcgt cagacgttag
     7741 aagcagaaat caaagaatta gaagctagtg gcgatacact gttagaagaa caattgaaat
     7801 tagaagcaga attaaaagat atccgtaaga atcaatggtc tgagaaaaaa gaaacgttta
     7861 ctgataagtt tgaattgcca gatgattgga aagatcaagc aacagataca ctgaatcaag
     7921 taggcgaaaa aatgtcagaa gctggatcac agttaggcaa attcttgaag aaaacattcc
     7981 aaactgtttc agaaacagtg aatgacaaca tggaatggaa agatgtcagc ttacgtgtcc
     8041 ctgggattgc gacaacgaaa tttgagcatg aattttacta tgaagctcct gcagcaagta
     8101 tccttgatat caaggcagca aacggaaacg tgacgttaaa aacgtgggac agcaacgatg
     8161 tgaaagttga agctaagatc aaactttacg gaaaaatggg tgctgaacca tttgaagcat
     8221 tctctgaacg gagccagatt gaggttaatg aagatcatat ctccttccaa attccaaata
     8281 aacgtgtgcg tgcagacctt gttttctact tacctaaacg agtatacgac cacgcagcta
     8341 ttaaacttct aaatggcaac atcatgattg aaacattgga agctaaagat atttatacaa
     8401 aatctacgaa tggtaacatc atcgtggacc aattaactgc aacgatgctg gaagtagaag
     8461 gtgtaaacgg caatatcgat attcgaaacg ggaacatctt agattctatt atcgaaacag
     8521 taaatggtac agtgacattt ggtgcgacag cagaaaacct ttctgtgtct ttggtaaatg
     8581 gtgatgtacg tcttacgatt aaagaagata atttgaaaaa agtagaagca agttcagtga
     8641 acggaaatgt caaagtcgct ttaccagatg gcatcggttt ggaaggacat gctaaaacaa
     8701 gtcttggaag tatcaacagc cgtctgtcca attacgaagt agttcgtgag aaaaaagaac
     8761 ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg tgaaatcgcg caagtgcaac
     8821 tttcaacgac aacaggaagt atctatttaa aagatacaga taaataaaga ggttgtgaaa
     8881 gagatgttca gccttgagca ataagaagcg attttgtaaa atagcttccc ctattttaca
     8941 aaattgtgcc ttattgccga tagtctatct cttgaacacc gtttaccaag agattatgaa
     9001 cctgccaatt atcgccgagt cacaaggaat aatttcagaa aaagctcgtc acgttttatg
     9061 aatatggtaa tttgtgaccg agaattttgc agaaagacac cgattaagaa gaaggttgtc
     9121 acaacgattt tcttcattta aaatagtaga acagaggtga gacatgatga aaaaaaagtt
     9181 gacaaaatct aataaaaatg ttgttttgac agggacttta gcaggaatag gagaatacat
     9241 cgggattgat ccgactgtta tccgtgtgct ttatgtattc ctgagcttag ttgcttttgg
     9301 aagtccaatt attttataca ttttattagc tttgattatc ccatctgcgc gttctgcacg
     9361 aacaaactat ggacataaca atgcatatta tgaaaaaaac aattatcgtg aacaaactcg
     9421 tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat gatgacgaat ggagtgactt
     9481 ttaatgtcgt actttcaacg tattttagtt aatacgttaa cattcatatc attggctgtt
     9541 ctttttcctg cgatggtcca tgtgagcagt atttggacgg ccgttcttgc cagcttcgta
     9601 ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga tactgtccct gccctttaca
     9661 ttagtcacat tgggattgtt tacgtttgtt atcaatgcag cgattcttaa aatgacctcc
     9721 ttttttgtgg gagcagaaca ttttagtttt tcttcattcg gtgcagcgtt gttagtagct
     9781 atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata acagtagaaa ataaaagaaa
     9841 gctgtgccga actatcggcg cagttttttt atttagaatc aggaatgcgt tttctattac
     9901 attggtaatt aaataaaaac gtttttttta attggtagta cttttggcta gacaatgata
     9961 aaatgataga gaacactcta atagaaatga ggtagcatga atgtcagaag ttgtaaaagt
    10021 tagccaatta gtgaaaaaat tatctttgga aatcgtgaca ggcgacgaga aaagcttaga
    10081 ccgtgtcatc gtaactgggg acatctcacg cccaggacta gagttgactg gttattttaa
    10141 ttattattcc cataatcgtt tacagctatt tggcagtaaa gaaatcagtt ttgcggaacg
    10201 gatgatccca gaagagcgtt tgatcatcat gagaagatta tgtagtgaag atatgcccgc
    10261 atttattatt tcaagaggat taccagctcc agaagaaatg atccaggcag caaatgaaaa
    10321 tgggatagct gtactgcgtt ctcccatctc aacttctcgt ttattaggag aattatccag
    10381 ttatttagat ggtcgccttg cccctcgaac aagtgtccac ggtgttttag tggatgttta
    10441 cggattaggt gtactgattc aaggtgacag tgggatagga aaaagtgaaa cagcgttgga
    10501 gttgatcaaa cgcggccatc gattgatcgc agacgatcga gtagatgttt atcagcaaga
    10561 tgaattaacg gtgatcggag aaccgcctaa aatcctggaa catctgattg aaatccgagg
    10621 aatcgggatc attgatgtaa tgaatctttt tggtgcgagt gcggtacgtg gctctatgca
    10681 agtccagttg gctgtttatc tggaagcatg ggcaaaagat aagaaatacg accgtctggg
    10741 ctctgaagac acgaccgttg aaatcgcgga agtgggcatt ccacaaataa aaatccctgt
    10801 caaaacagga cgaaacgttg caatcatcat tgaagtagcc gcgatgaatt tccgtgcacg
    10861 tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga ttaagtcgct tgattgaaga
    10921 aaactcaggc aacgactagc aaggagtgac aatatgttag cacaaatcaa tcgagttgct
    10981 tttgagcttg tagggattcc tatctactgg tatgcattga tcatcgtatc aggtatcatt
    11041 atcgctatgt ggcttagcag cagagaggct gttcgggtag gattaaaagc agatgatgtg
    11101 acagatttta tgttgtgggg attgcctatc tccattattg gcgctcgctt atattatgtt
    11161 ttatttgatc ttccccagta tatagctgat ccaatccaaa tattcaatat ccgttcagga
    11221 ggcttagcta tttatggcgg actgattgca ggcgcgatca cgctgtattt ttttactaaa
    11281 tatcatttta tttctttgtg gactttttta gatatcgctg caccaagtgt attattagca
    11341 caggcaatcg gtcgttgggg aaatttcatg aaccacgaag catatggacc agacacgacg
    11401 cgaagcttct tagaaagtct gcatttaccg caattcatca ttgataatat gtatatcgaa
    11461 gggatctatc gtcaaccaac atttctttat gaatcacttt ggagtttgat tggatttatt
    11521 ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa aaggcgaagt ccttttatgt
    11581 tatctaatgt ggtatagttt cgggcgtttc tttattgaag gattacgtac agacagtctt
    11641 tactttttag gtgtgatccg tatttctcaa ttattatctg ctgtgttatt tgtcggtaca
    11701 ttgattctat ttatttggcg cagaaaaaaa ggaaatttac cggattatga ccgctcatta
    11761 ggaaaaaatc aacaaattat ctgattttta gagcgttaaa aatgaaaaaa atttcaggag
    11821 tggtaaaatg aattatagag tagcggtttt aggaccaggc tcttggggta ctgctttggc
    11881 acaagtcctc gccgaaaacg gacatgacgt tcggatttgg ggacatcgtg cttcacaagt
    11941 agaagaaatc aacacccaac acacgaatca acgttattta ccagatcatt atctacccga
    12001 ttccatccaa gcattcacca atatggaaga tgcagtaaaa gacgcagatg cggtgctatt
    12061 cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa ctagtgccta aattgaatac
    12121 aaagccagtg atcatccatg caagtaaagg attagaacag ggaacacata aaaggatctc
    12181 tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa gctgttgttg ttctttctgg
    12241 accaagccat gcagaagaag tagcaaaaca tgacatcact acgatcacag ctgcaagtac
    12301 agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg aataattatt ttcggatcta
    12361 taccaatcct gatgtgatcg gtgtagagat gggggcagct ttgaaaaata ttattgctat
    12421 tggtgctggc gctattcacg gcttagggtt tggcgataat gcaaaggcag caatcatgac
    12481 gcgcggatta gctgagatca gccgtttagg tgtagcaatg ggtgccaacc cgctgacctt
    12541 tatcggatta agcggaatag gcgatctagt cgttacttgc acaagtgttc attctaggaa
    12601 ctggcgcgca ggcaacttgt taggacaagg gcagccccta gaagaggtgc tagccaatat
    12661 gggaatggtc gttgaaggtg tgaatactac ccaagcagcg atggaactag cccaaatgat
    12721 ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg ctgtatgaac aaaaagaaat
    12781 caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa aaagaaaatg aattctcatt
    12841 aacaattttt tgaaggagcg aatgtatatg aaagttaaaa aagcagttat tccagcagca
    12901 ggattaggaa caagattttt accagcaaca aaagctatgg caaaggaaat gttgccaatt
    12961 gttgataaac caacaatcca gttcatcgtt gaagaagcgt tgaaatcagg tatcgaagac
    13021 atcttgatcg ttacaggaaa agctaaacga ccaatcgaag atcactttga ttcaaatatt
    13081 gaattagaaa caaacctaag tgaaaaagga aagaccgaat tattgaaatt agtagaagaa
    13141 acgacagatg tcaacttgca tttcatccgt caatctcatc caaagggttt aggacatgcc
    13201 gttcttcaag caagagcctt tattggaaat gaaccatttg tcgttatgct aggcgatgat
    13261 ataatggaag acgaagtacc attgacacag caattaatcg atgattacga acgtactcat
    13321 gcttctacga tcgccgttat gcaagttcct catgatgaaa cttcaaaata cggaattatc
    13381 aatccaggag aagtattgga agatgggtta tacaatgtca aaaactttgt tgaaaaacca
    13441 gatccaagca aagcaccaag tgatttagct attattggac gttacttatt gactccagaa
    13501 atttttgatg tattgtccga gcaacaacca ggagccggaa atgaaatcca actaactgac
    13561 gcaatcgata cattgaataa aacacaacgc gtatttgccc gtaaattcac aggtaaacgt
    13621 tacgatgtcg gtgacaaatt tggttttatg aaaacaagta ttgaatatgg tctaacacat
    13681 ccagaagtag gtgcaccttt acgtgagtac atcctgtctt taggtaaaac attagaagca
    13741 gaaggaagca agatcgatca taatccaaaa ggaataacag aaatcaaaga agaaaagaaa
    13801 aaataaccct tttttctaac gagcttcagc tgctaattga ataaaaagta gttgaagctc
    13861 gtttttattt gtttaaaaaa cacgaaaatg ctatccttat agaaaagaga gacaagaagg
    13921 gagtttgatc atgtcaggag gagaaattgc aggattgatc gcagcaatcg cctttgcgat
    13981 acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct aaaactttag aaaaagtaga
    14041 tcagactgta gcagaagcaa atacgacgat cgaaatcgtc acaaaagacg tagatctttt
    14101 gtctagacaa gtagaaggct tactggtcaa aagtaatgaa ctgctcttgg atatcaatgg
    14161 aaaagtcgca acgatcgatc cattgttcac agcagttgct gatttaagtg aaagtgtatc
    14221 agaacttaat cattctagta aaaacatcgc gacgaaagtc ggcggaatcg gtaaaacgac
    14281 agctaaagcg acggttgccg gaaaagtcgg tgggacagcg atgaaatttt ttaaaaacaa
    14341 aaaacatact gaaacgacag gaggaaaata aaaatggcta aaaaaggtgg atttttatta
    14401 ggtgcagtag tcggaggcac agccgcagca atcgccgcgt tattgttagc accaaaatct
    14461 ggtaaagaac ttcgggatga attagctgcc caagcggatg atttgatgga tatggcttca
    14521 gattacacag atatggcgaa agaaaaatcg actgagttaa caggtattgc aaaagaaaaa
    14581 gcgcaagatc tttctcaaca agcaggtgat ctagcaggta gtgtcaaaca aaaagtaagt
    14641 gaatcagcag atgaagcagg aagtgaatct gacgatatct tgttaaatct taaagaacaa
    14701 tcagctgact tatctaatcg tttcaaaaaa acggcagaag atatgaaaaa acctgcagat
    14761 gaattaggaa gtattgctaa agatacttca gaagatatca tgattgatgt aaaagacgct
    14821 gctggtaaag caggagatac tgtcaatgaa ggaatgtcag aagcaaaacc agttgcagat
    14881 gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga aagaagctaa aaaagaaata
    14941 aaagatacaa tgacagattc atctacaaat cactaaaatc acaaatttat agaataaaaa
    15001 ggtgggagcg gactagctct cacctttttt actcttattt ttctaaaaca agtaattctt
    15061 ttggtgtagt cgtgaatggc acacagccat cgcttgtcac atatacacag tcttcgatac
    15121 gtacccctac tttttcagga atatagattc ctggttcaag agagaagcac atcccttctt
    15181 cgattactaa gtcatttcct tctacaaggg aagggaattc gtggacggtt gttccgatcc
    15241 cgtgacctaa acggtgattg aaatattcgc cgtagccata gctgttgatc acgtttcgtg
    15301 cgatttgatc aagttcacct gctgtcacac ctggacggac ggcttcttgt gcggcaagct
    15361 gtgcttcaag agtgatgttg tagatttttt cttggaagtc ggttggtttt tgataagcaa
    15421 ttgtacgagt agcatcactg caatagccat tccaaacaac gccaagatcg aataacacaa
    15481 gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg agggcttgca gcatttgctc
    15541 ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc ttgttttttc aattggtatt
    15601 caatttctgc aatgatcgct tgttcagtca cgccttcttt cactgcttta aagccaatct
    15661 caaatgcaac atcggcccat ttaccagctt cgatcagttt gtctttttcc tctagagttt
    15721 tgatcagttg tagacgttgg atgatggaag ttagatcagt gtaagaatcc gttcccgtca
    15781 agatatcaga gatagcatcg aagcgctcga cagtcaaggc gttcttttca atccctacat
    15841 tgccaggctg accatagcga gcacgaagtt cttttccgat gatctcccaa gggttttcgc
    15901 tgtctaaata gccacggaca tcatacgccc aactactgtt ttgagcatct tcaacttcca
    15961 aagcaggagt gaaaaggaaa ggatcttgtt caagagggat aaaaagagct agtacacgct
    16021 catgaggatc actttcgtat ccggaaaagt aagcgatgtg gccagggtcg ctgatataag
    16081 ccaattcgac ttggtgttgt gccatccatt gacgtagttc attgattttt acgttattca
    16141 tttggcaacc tactttctaa attaattttg tattcaataa ttgtaccact gatttattaa
    16201 aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc atgaaaacag agataaaacg
    16261 gttgcaattc ttgagattat ttgatattct attttagaaa tgtaagacgt attttcagaa
    16321 aacgtataaa atatgaatag gagaacacaa atggaaaagc aaacaatcac tatctatgat
    16381 gtagcgagag aggcaaatgt ttcgatggct actgtttcac gtgtcgtaaa tggcaatcca
    16441 aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta tcgatcgctt ggattatcgt
    16501 ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa caacagttgg tgtaatcatc
    16561 cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg gtatcgatga tgtggcaaca
    16621 atgtacaaat acaatattat cttagcaaat tcagatggca acgaccaaaa agaagtcaat
    16681 gtattgaata acttactggc taagcaagtg gacggcgtga tcttcatggg tcaccatatc
    16741 acagacgaaa tccgtggaga attttcacgt tcaaaaactc ctgttgtatt agctggctct
    16801 attgatccag atgaacaagt aggtagcgta aacattgatt atgtatccgc aacaaaagat
    16861 gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg cttttgtcag tggtgcgttg
    16921 atcgatccca tcaatggaca aaatcgtctg aaaggctata aagaagcatt aagcgaaaat
    16981 aacctaccat atagcgaagg attgatcttt gaagcacaat acaacttcaa agatggtcta
    17041 tctctagtcg atcggatcca taatagcggt gcaacagctg tctatgtttc agatgatgaa
    17101 ttagccatcg gtattttgga tggtttatta gaccgtggcg tgaaagtacc ggaagaattc
    17161 gaaatcatca caagtaacaa ttcactactg acagaagtag ctcgtcctcg tttaacaagt
    17221 atcacacaac cattatacga tttaggggcg gtatctatgc gcctattgac gaaaatgatg
    17281 aataaagaag aagtcgaaga aaaaacgatt atcttgcctt atggaatcga agaaaaagga
    17341 tctacaaaat aataaaagga caaccattca gaggttgtgt gcctgccgtt taactctgcg
    17401 ttacaaggag caatttcaaa aaacagcatc tcatgtttga tgaatattgt gactcgtgat
    17461 tcggagttgg caggagaaca ccgtttattt gggaaaaggg gttatgacaa aattttgtta
    17521 taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt gaaattacat ttatcgtata
    17581 taaaacaatc cgtgtataga tgctgcattt tgttcaccac catcaaaaaa ataagggact
    17641 gtgacaatac tattgtcaca atcccttatt actgtgtatt ctattaaatc aatcatcttt
    17701 ctcatctgat tctttagttg cttgattcgc tcttgcataa gctgaagcgg tgttccagta
    17761 atctgtatag ttttcatcgg taccgccaac gccaaattta aaggcgcttt tcttcggtcc
    17821 atttttcgcc catagcgatt caactgtttt tcctggtgtt tggatggatt ttttattgac
    17881 agtaactttt ccaggtgttt gaccagtaaa tccagatact tttttcttca tcacatcaga
    17941 tgataattcg aatttctcat cagtgccaaa aatgtcaggt tttgcttggt aagcttgatt
    18001 gatcagattg gcaagataat tagccgaacg tatccttgct ttgctgtcca ttccagtatt
    18061 gtcatcgtgc cctgcccagc tactgatggt aatagacgga gtagagacaa ttagccatga
    18121 atctcggtat tcattggtag aaccagtttt tccgacccaa tctgcttttc ctagatttcc
    18181 gtttaagctt gagatggcat ctttgaatgg tgtagtgatt tttgcattga tcacactacg
    18241 catcatgtca ttcatgatcg atgccgttgc ttccgaatag atccggacag agttgctttc
    18301 gtgtttataa attacatttc ctgcgttatc tgtgatggaa tcgatgatat agccttcttc
    18361 atagacgccg ccgtttgcta aagcctgaaa tccgttggtc tgttgaagcg tcgtgacatc
    18421 tactgtacct aaaggagcag attcaacgcc ccaattgtca tttgccgggt agttcatctt
    18481 tgccaagtag tgctcgtaag catattgttt cgatccaccg gaattcaaca catcttgata
    18541 taagtgataa gccgggatat tgttggacca ttctagtgac tcacggacgg tttggaaggt
    18601 gttcgatcct tcgttagtag cattgacgat tttctcgcca gcattttctc cttcttgcca
    18661 agttgttgca taatctgata cacgggattc tgatccgata agccccatgt cgattgcagg
    18721 tccataaaca agaactggtt tgattgatga gccagcttga cgttctgtat cgaaggcatg
    18781 gttattttga ttctgactgt aattccgtcc gccaacgaat ccgtagatgc ggccagttct
    18841 gttatccatc aatacatttc cggtttctac ttgactgcca gcaccatcat ctaacatgta
    18901 tccataattt tcaacgccat tttgcaatgc agcatagaca tctttatcga ttgtcgaatg
    18961 gattgtatat cctttatttt ggatcgtttg ctgtgccttt tgataataag cgtcactgac
    19021 cgatgaatca gacatatcag cgttgtcttt ttcagccaat tgttgtgcaa tgattcttgt
    19081 tgcttcattc atcacagtat aatacaagaa ttctctgtca gtctgttctg cgatttgttg
    19141 tggtagaaag tcttttgtaa ggtcataggc ttttgcttct tcgtattgtt ctttcgtaat
    19201 ttggccttca cgatacatac tgaataaaac aaaatctttt ctggcaaggc cggcagaaag
    19261 atcttctttt agcgcgcctg tgttcgtgta tggactatac gtgatcgggc tttggggtaa
    19321 tccagcgata taagcagctt gcggcagcgt cacatctttt gcatctacac caaagattcc
    19381 ttttgcagct tcctggactc cagcaatgtt ttgtccctta ttattacgac caaatggtga
    19441 gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag tatttttcta cttgtgcaga
    19501 aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca tctgtaagga tctgctgttt
    19561 gactaattgc tgtgtcaaag ttgatccacc agaagagcca ataccagtag cttcagatac
    19621 taatgctcgt aaaacagctt taggaacgta accttggtgt ttatcgaagt attcatcctc
    19681 tgtgctgata attgcagttt tcaataaagg tgaaatctta tcagatgaaa tagtcgtgcg
    19741 catcagatct gtctggattt ttgatatctc tgtattatct gcataaacaa tctttgacgt
    19801 ttctgtaatg tttcctagtt ctgtctgcag tgctttttta gtcggcaatt gtgtgtcttc
    19861 gacgagatag gcaaaatagc cagcgcctat tccaatacct agagctgcga acatcaaaag
    19921 aagaacagta ataaaaacag tcaatgattg aaaaactcta aaaataatct taggaacaaa
    19981 ccatttttct ttttttcttc tattctgttt ttttcgagta tttttgcgtg gcaaaagggt
    20041 cacctcaact aatggtttta tttcgctaat tatagcaaat aatagaacaa aaaaacagac
    20101 aaggctgttt ttggcaggaa tttctttttt aaaagagtta aaacgtgaga aaatgatctt
    20161 tattataaaa gagttc
//
LOCUS       DAGCWU010000039        20082 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.039, whole genome shotgun sequence.
ACCESSION   DAGCWU010000039 DAGCWU010000000
VERSION     DAGCWU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.039"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            195..1961
                     /locus_tag="MBZ71_001105"
     CDS             195..1961
                     /locus_tag="MBZ71_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBT4429074.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1976..2413
                     /locus_tag="MBZ71_001106"
     CDS             1976..2413
                     /locus_tag="MBZ71_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4429075.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2426..3577
                     /locus_tag="MBZ71_001107"
     CDS             2426..3577
                     /locus_tag="MBZ71_001107"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBT4429076.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(3639..4337)
                     /locus_tag="MBZ71_001108"
     CDS             complement(3639..4337)
                     /locus_tag="MBZ71_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBT4429077.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(4330..5682)
                     /locus_tag="MBZ71_001109"
     CDS             complement(4330..5682)
                     /locus_tag="MBZ71_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBT4429078.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(5905..7305)
                     /locus_tag="MBZ71_001110"
     CDS             complement(5905..7305)
                     /locus_tag="MBZ71_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBT4429079.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            7466..8203
                     /locus_tag="MBZ71_001111"
     CDS             7466..8203
                     /locus_tag="MBZ71_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBT4429080.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            8328..8807
                     /locus_tag="MBZ71_001112"
     CDS             8328..8807
                     /locus_tag="MBZ71_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBT4429081.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            8804..9571
                     /locus_tag="MBZ71_001113"
     CDS             8804..9571
                     /locus_tag="MBZ71_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429082.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEELVVYSRSQRRRHS"
     gene            9801..10370
                     /gene="lepB"
                     /locus_tag="MBZ71_001114"
     CDS             9801..10370
                     /gene="lepB"
                     /locus_tag="MBZ71_001114"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBT4429083.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10474..11076)
                     /locus_tag="MBZ71_001115"
     CDS             complement(10474..11076)
                     /locus_tag="MBZ71_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4429084.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            11182..11651
                     /locus_tag="MBZ71_001116"
                     /pseudo
     CDS             11182..11651
                     /locus_tag="MBZ71_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11757..12341
                     /locus_tag="MBZ71_001117"
     CDS             11757..12341
                     /locus_tag="MBZ71_001117"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBT4429085.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            12344..13417
                     /gene="prfA"
                     /locus_tag="MBZ71_001118"
     CDS             12344..13417
                     /gene="prfA"
                     /locus_tag="MBZ71_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBT4429086.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            13410..14249
                     /gene="prmC"
                     /locus_tag="MBZ71_001119"
     CDS             13410..14249
                     /gene="prmC"
                     /locus_tag="MBZ71_001119"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBT4429087.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            14242..14421
                     /locus_tag="MBZ71_001120"
     CDS             14242..14421
                     /locus_tag="MBZ71_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429088.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            14443..15468
                     /locus_tag="MBZ71_001121"
     CDS             14443..15468
                     /locus_tag="MBZ71_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBT4429089.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            15643..16887
                     /locus_tag="MBZ71_001122"
     CDS             15643..16887
                     /locus_tag="MBZ71_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBT4429090.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            17040..17987
                     /locus_tag="MBZ71_001123"
     CDS             17040..17987
                     /locus_tag="MBZ71_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4429091.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            18120..18749
                     /gene="upp"
                     /locus_tag="MBZ71_001124"
     CDS             18120..18749
                     /gene="upp"
                     /locus_tag="MBZ71_001124"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBT4429092.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(18888..19130)
                     /locus_tag="MBZ71_001125"
     CDS             complement(18888..19130)
                     /locus_tag="MBZ71_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429093.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            19251..19323
                     /locus_tag="MBZ71_001126"
     tRNA            19251..19323
                     /locus_tag="MBZ71_001126"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19284..19286,aa:Thr,seq:cgt)
     gene            complement(19442..20080)
                     /locus_tag="MBZ71_001127"
     CDS             complement(19442..20080)
                     /locus_tag="MBZ71_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBT4429094.1"
                     /translation="MNNILGKKIELKKGILGTIVVVFGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         6830 a         3579 c         4088 g         5585 t
ORIGIN      
        1 agttattgtt tgcatattat aaaaaaatta aaataacagt tttatttaaa attgaagttt
       61 tctgataccg aaatcttttt attttgctga catactcaaa cctttggcac aaagtttcgt
      121 gccaaagtac gactatttct ctttttagta agcggataca attagagtat caaaaggaga
      181 gagggagaga caaaatggaa cggacgattc ccaaaaacaa catttcgcta aaagcgaggg
      241 tacaaaaatt aggcagtact ttatcaagca tggtcatgcc aaatattgga gcactgattg
      301 cctggggtgt attaacggct ctatttatcc cagatggtta tttaccaaat gaggctttcg
      361 ctaccatggt aagcccaatg ctaacttatc tgattccttt attgatcggt tatactggcg
      421 gaaaagtaat tgctggtgac cgcggatcag ttgttggggc aattgcaacg atgggtgtga
      481 ttgtcggaac agatattcca atgatgctag gcgcgatgat catgggacct cttggtggat
      541 atgccatcaa gaaatttgat cagctattcc aaaaacggat caaatccggt tttgaaatgc
      601 tagtcaataa tttttcagca ggtcttatcg gttttggatt agcgttatta ggtttttctg
      661 cgattggtcc agttgtcgat gcattgactc aagcaatggc aaaaggtgta gagatcattt
      721 tatctgccca tttgattcca ttgacaagta tttttattga accagcaaaa atcttatttt
      781 tgaataatgc aatcaatcat gggattttga caccattggg aactgaacaa gtagcagagg
      841 ctggaaaatc tattctattc ttgcttgaag caaatcctgg tccaggactt ggcgtattac
      901 ttgcttttac tttgtttggc aaaggagcag caaaatcttc agcaccagga gccatgatca
      961 ttcacttttt aggcgggatc catgaaatct attttcctta cgttatgatg aaaccgttat
     1021 tattcttatc agttataggc ggaggaatgt caggaagttt cattttccaa ttgatggatg
     1081 ctggactaag agcgccagct tctccagggt cgatcattgc gattctagcg atgacaccgt
     1141 tcaattctta ccttcctgtt attttgggcg tgattgttgc cgcagctgtt tcgtttggta
     1201 tttctgcagt aattttgaaa gcggatgcga aagatacaaa tgaagacttc gaaaaatctg
     1261 ttcaagagac aaaggcagct aagcaagctt ccaaaggtgc ccaaggtatt cctcaaggag
     1321 ctacattctc tggcgtacaa aaaatcattt ttgcttgtga tgcaggaatg ggatcaagtg
     1381 cgatgggcgc ttcgatcttg agaaaaaaag tacaggaagc agacttgcct caaacggtga
     1441 ccaatcaagc aatcagccaa ctgacagatg actcgaatac attgatcgtt actcaggtcg
     1501 aactacaaga aagagcaaaa caaaaagcac cgagcgcgcg ttttgtctca gtagaaaact
     1561 ttttgaattc acctcgatat gatgaaatca ttcaagaatt aagcagcaag cagaaaacat
     1621 cagtttctga aattgagaat gagccagcag aagagtttct ttcagcaaac atcaaacaaa
     1681 tcaaagagat tctacttgtc cacgatgatc gttccggatc agcaacaatg gggatgaccg
     1741 tgctcaaaga tatattagac aagaaccatt taactatccc gttaagaaaa gtcaatatcc
     1801 aagatttaaa agatgaacct gattcactga tcgtagccaa agaaagttta gcggaaaaag
     1861 tacagatggc tgcacctcaa tccattcatt tatcagtcaa cagcatggtc aatccgcaaa
     1921 aatacgaatc gattgcacaa tatttgaaac agacagctta agggaggaaa acagcatgca
     1981 aagtttgcca ttcgacatga tcgagctcaa tcgatctttt aaaaccaaag aagaagcgat
     2041 ccgctattgc ggtcagatgc ttgtagaagc agggtgcgtt gatgaggaat atatcgatgc
     2101 catggtagat agagacaaaa tgttatctgt ttatatgggg aattttatcg cgattcctca
     2161 tggaacagat gaagcaaaaa aatatgtgaa aaaatccggc atctgtgtcg ttcaagtacc
     2221 agatggtgta gattttggta ccgaagaaga agaaaagatt gcgactgttt tgttcggtat
     2281 tgcaggagtc ggagatgagc atttgcaact tgtacagcaa atcgcacttt attgcagtga
     2341 tatggacaat gttgtccagc tagcagacgc gctcagcaaa gaagaagtaa cggaaaatct
     2401 agcaattgct taaggagaga gaaaaatgag agcagtacat tttggtgcag gaaatatcgg
     2461 tcgaggattt attggagagg ttttggcagc taatggattt gagattactt tcgtagatgt
     2521 gaatcacacg atcattgatg ctttaaatga aagaaaagaa tacgaaatcg aattagcaga
     2581 tgaatcaaaa gaaaaaatca ctgtaaaaaa tgtcaaagga atcaacaatc aaacaaatcc
     2641 agaaaaagta atccaagaaa tcgctgaagc agatttagtt actaccgcca ttggcccgaa
     2701 tatcttacct tttattgctg agttgattgc aaaaggtatc caagaaagag aaaaggaagg
     2761 aagcacaaca tctttagata tcattgcctg tgaaaatatg attggcggtt cagcatttct
     2821 agaaaaagaa gtgtataaat atcttccgga aacatcattt actgacaaat atatcggctt
     2881 tccaaatgca gcagtcgatc gaatcgtccc attacaacac catgaagatc ctttattcgt
     2941 ccaagtggaa cctttcaaag aatgggtcat tgacgatagc caaagaaaaa ataaaaaaat
     3001 ccaattaaaa ggggttttat atgtagatga tctagaacca tatatcgaac gcaaactatt
     3061 tagtgtcaat actggtcatg cgactgtcgc ttatacaggt gccttgttag gttaccagac
     3121 cattgatgaa gcaatgcaag atgcgttagt tgtggcacaa ttaaaatcag ttttgcagga
     3181 aacaggaagt ttgctgattg ccaaatgggg gtttgatgca gagcagcatc atgcgtatat
     3241 tgaaaaaatc attcatcgct tccaaaacaa aaatatctct gatgcgatca cacgtgtcgc
     3301 tcggacacca ttacgtaagt taggttatca agaaagattt acacgtccag tgagagaact
     3361 acaagaacat aacttgactt gcccacattt gacggcaaca atggggatta tttttaatta
     3421 ctatgatcct gaagatgaac aaagccgtca attgcatgaa atgaagatcc atgaaaactt
     3481 ggaacaattg attcaagaag tcacaggaat caatgatccg aaaacaatcg ggaatatcaa
     3541 gcaaaacgtg aaccgttatg ccaagcaagt cgcttaaaat aggatacgaa aaaggggaat
     3601 gtgacgaaat ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga
     3661 tagggcagat ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct
     3721 gccaggttct catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg
     3781 taaatgacac cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc
     3841 aaaggtcgtt caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa
     3901 gtttcattga attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga
     3961 cttaacgtgt agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg
     4021 tagtagtgac ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg
     4081 atatagttga tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca
     4141 aagtcttgac caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt
     4201 atactaacga tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc
     4261 aaaaggtttc cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg
     4321 agttcataat tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt
     4381 acgaagctgc aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc
     4441 tttgattttt tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat
     4501 cccggcaact tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc
     4561 tgggatatcc atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat
     4621 tccgtctgcg taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc
     4681 aatcatatcg attacttgat tgatacctac aggatttttg acaagtacta atgtacattt
     4741 tttcccattg atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc
     4801 ttgtttgatt ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc
     4861 attgtagaca ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa
     4921 atctgctgac acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg
     4981 tttgaagtca caatttggac agtaatattt ccctaagttt gcataagtga tcattttata
     5041 atgcaagatg tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg
     5101 ttcacgatct tcttcatggt caaagccata atattttcta ggattgactg tttcgataga
     5161 attgaagatt ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc
     5221 gtctacaatc attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa
     5281 aatatttgta aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc
     5341 ttcatctatt tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa
     5401 agtcgataca attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa
     5461 ttgctggcgc aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt
     5521 gacaacgacg acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag
     5581 cgctagttta cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga
     5641 cgtttttcca actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac
     5701 gatttgctga cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc
     5761 ttttcttatt cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat
     5821 gatttaacaa aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta
     5881 ttccctctta cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata
     5941 gcctagatct cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg
     6001 cccaataaac atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat
     6061 ccctaaagat aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag
     6121 cgtttgttct tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag
     6181 cataaagaca acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt
     6241 gatgttatct tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt
     6301 tgttcgaatc ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa
     6361 aatgatcagt gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg
     6421 agtcgtaatc gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa
     6481 taaatggtcc ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa
     6541 agctaacaca gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg
     6601 tttgtataat agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa
     6661 aatcaaaaac atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc
     6721 cccagtgacg tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa
     6781 aacggcatca cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt
     6841 cccaaaaagt aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat
     6901 accagacaaa cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat
     6961 ccgtcgttta gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac
     7021 ttgaaataat atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa
     7081 agtcgatagt ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga
     7141 aacatggcta tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt
     7201 catcaatata taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt
     7261 ctgtcctcgc cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg
     7321 acaatagtcg tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa
     7381 cagataacaa tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga
     7441 aaaaagaagg aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa
     7501 aaatcaaaaa ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga
     7561 agaagtaaag cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt
     7621 ggaattcatg tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc
     7681 taaaagtgag tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga
     7741 gaaaactgac catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa
     7801 aatggtttta acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg
     7861 cttcaatatg cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt
     7921 tggaagtgcc ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat
     7981 ggatatccaa aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg
     8041 ctatgaacgc tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg
     8101 ttgcgtgttc gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa
     8161 agcttactta aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa
     8221 acgtaagaaa aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt
     8281 agtaaagtaa ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag
     8341 cacctatttt gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag
     8401 gtattgtgaa tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa
     8461 caacaccaac tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa
     8521 ccactacgac aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata
     8581 caactgacag ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa
     8641 ccaatccaag tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga
     8701 ctgtagatga taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt
     8761 caggaaatac ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga
     8821 ccttatcaac ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa
     8881 ccgtggatat acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg
     8941 actttggcaa aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct
     9001 tgtttatttt actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga
     9061 tgtcactcag ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga
     9121 atcgatagat aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta
     9181 tgacgaagat tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga
     9241 gatcaaaaca aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta
     9301 tccttattac tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag
     9361 ctactcggac atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga
     9421 acctgtcgga ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga
     9481 gccttatcgt ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac tagtggttta
     9541 tagtcgtagc caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg
     9601 cgataaaaac gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt
     9661 ttatataacg agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca
     9721 ttgtagaata aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa
     9781 atacaagtag gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg
     9841 tattagcggc tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc
     9901 attcgatgga ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact
     9961 atgaacgaat ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta
    10021 aacggatcat cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta
    10081 atggcaaaaa gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata
    10141 agttacaagg agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca
    10201 cctttacgag tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata
    10261 accgcttgat ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag
    10321 gaaaagttgt tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata
    10381 aaaagaagct gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt
    10441 cttggagttg ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa
    10501 aagcgaagcg tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc
    10561 agagacaaag cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg
    10621 gactttcgga tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc
    10681 aggagctaaa atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag
    10741 tacaagtgat agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc
    10801 gtggatcgtt cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag
    10861 catagtttct gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact
    10921 aacaagccca gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag
    10981 ataagtagtt agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata
    11041 atggttagcg atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt
    11101 tgaaatttta acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa
    11161 aagggagaat ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga
    11221 aagtgtgtcc gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg
    11281 gaaaaatata tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa
    11341 tatttaggct ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt
    11401 gtatgggaat aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag
    11461 cagaaggtta cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc
    11521 actactctat atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg
    11581 aagtttttcc aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg
    11641 aacaaaagtg agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa
    11701 aaatgctgaa aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg
    11761 cacaactatt ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag
    11821 tcgcacataa ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata
    11881 caagagatgg tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga
    11941 tatttgaaga aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg
    12001 tgctgatcga tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg
    12061 tggacgaatt ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt
    12121 tatttgaagg atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa
    12181 tctgttggtt ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg
    12241 tttatgaagg cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa
    12301 aacattactt tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca
    12361 agcaattgaa gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc
    12421 tgatacaaag cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga
    12481 agtttatcgt cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg
    12541 tgaaaaactg gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa
    12601 agaaaaagaa gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga
    12661 cgataaaaat atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt
    12721 cgctggcgat cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga
    12781 agtgatggaa gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac
    12841 cggagacaac gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt
    12901 tccttcaact gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc
    12961 agaagctgaa gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca
    13021 tgcttcaggt gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca
    13081 cttaccaaca gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga
    13141 aaaagcaatg aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag
    13201 cgagtatgat gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg
    13261 gacgtataat ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa
    13321 attagatcag attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga
    13381 tcaaacatca aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt
    13441 cacgggcttc ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat
    13501 ttttggaacg aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt
    13561 cagaagaaga acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac
    13621 ctcagtattt gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa
    13681 cactgatccc tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg
    13741 gtgtaccgtt agaagtgata gatattggga cagggactgg agcaattgct attagtttga
    13801 aagcagcaag aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag
    13861 tagcgaaaga aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat
    13921 taggaccagt aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc
    13981 ggaatgaatg ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt
    14041 ttgctgagaa cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac
    14101 taaaatcaaa tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa
    14161 aaatatttca gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca
    14221 atgaacgaat gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt
    14281 tttattataa aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa
    14341 gtttatccac tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc
    14401 agacaaaata agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat
    14461 gatgaaacag aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt
    14521 cctaccgaaa cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa
    14581 gtgttttctg taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt
    14641 gaacaagtaa aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac
    14701 ttctggcctg gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt
    14761 gtgacgggag gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa
    14821 gtaatcgaac tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca
    14881 agtccaacga ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt
    14941 gatgatggag ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca
    15001 gccatgccta tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc
    15061 gaatctcctg ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct
    15121 gggatgaaat acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat
    15181 tggtcaactg cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc
    15241 cccgaaattg cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac
    15301 tcagtagaag cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca
    15361 cttggattag atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac
    15421 atgaatcgct tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat
    15481 tgtcgcattc ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa
    15541 aactaagcgt aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg
    15601 ggaaaaataa aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg
    15661 tttgaccctg atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg
    15721 gagctgattg cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc
    15781 ttgacaaata aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt
    15841 gtcgatatcg ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt
    15901 gcgaacgtcc agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta
    15961 gaaccagggg acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga
    16021 tcaccagtca acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca
    16081 acagaagtta ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg
    16141 atcgtagcag gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc
    16201 gcagatgaag taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca
    16261 gcaggacttc atcctaatcc agttccttat gcagatatca caacgactac cactcataaa
    16321 acattgcgtg gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa
    16381 ataaatagtg cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt
    16441 aaagcagtag cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc
    16501 atcgccaatg caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt
    16561 tcaggggcga cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga
    16621 aaagaagcag agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct
    16681 tttgaaacac taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact
    16741 actcgcgggt tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta
    16801 caagcaaaag atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact
    16861 gaaaaatttc cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc
    16921 aatgttgggc ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg
    16981 ctctctagta tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca
    17041 tgactgatcg tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc
    17101 atccgcgtat ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag
    17161 aatcacctgt gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat
    17221 ggggaaaact caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta
    17281 ttttttatat tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga
    17341 tcagagaact tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac
    17401 cagaagcaaa atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata
    17461 aattagccaa gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag
    17521 gtggtaacat ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat
    17581 ccaagcaatt gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg
    17641 aattcggaga aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata
    17701 cgacgaatga agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg
    17761 aactaaatga tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg
    17821 aaggtgaggc ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct
    17881 tccaaggaat catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc
    17941 gtgcagcaat ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc
    18001 gcaggaaact tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca
    18061 atcacttgat tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa
    18121 tgggcaaatt tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg
    18181 acaaaaactg cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg
    18241 catatgaagt atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg
    18301 aaacaacaca aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag
    18361 gtattggaat ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg
    18421 gtctttatcg agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag
    18481 acattgatgc acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa
    18541 tcatggcaat cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg
    18601 tagcagcacc tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata
    18661 cagcttctct agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg
    18721 cgggtgaccg tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt
    18781 tgtcacatcc ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt
    18841 cgtttgcata taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt
    18901 agttctttgt agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct
    18961 gcagtttcat caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt
    19021 tgcgacttgc cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt
    19081 tccgatggta cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc
    19141 tttcacatca attataccac gttttatgaa aaatatcatg aaattaaact aattttccat
    19201 cggactcttt acaatcagcc aaatagagta tataatttct tttggataag gccagcttag
    19261 ctcagttggt agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg
    19321 gcattaaaag agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt
    19381 tgtttaaagg aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa
    19441 tttaggagaa taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc
    19501 aacttgtaat gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc
    19561 cttgtatcaa aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt
    19621 aatggataaa tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac
    19681 tagaaaaaat agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca
    19741 aaataataaa taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt
    19801 tcaaaatttg ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat
    19861 atttggctat atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt
    19921 taccaaaaac aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa
    19981 tggatggcgc aaatagcaac cctaacaaac cgaatacaac gacaatagtg cccaatatcc
    20041 cttttttaag ttctattttt tttcctaaaa tgttattcat ga
//
LOCUS       DAGCWU010000040        19978 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.040, whole genome shotgun sequence.
ACCESSION   DAGCWU010000040 DAGCWU010000000
VERSION     DAGCWU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.040"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..195
                     /locus_tag="MBZ71_001128"
     CDS             <1..195
                     /locus_tag="MBZ71_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBT4429095.1"
                     /translation="LKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPYLLIKTSDEG
                     FSSFAPTVCDILADDWAIVE"
     gene            192..953
                     /locus_tag="MBZ71_001129"
     CDS             192..953
                     /locus_tag="MBZ71_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBT4429096.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            1333..1842
                     /locus_tag="MBZ71_001130"
     CDS             1333..1842
                     /locus_tag="MBZ71_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBT4429097.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(1917..2456)
                     /locus_tag="MBZ71_001131"
     CDS             complement(1917..2456)
                     /locus_tag="MBZ71_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBT4429098.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(2596..3207)
                     /gene="rpsD"
                     /locus_tag="MBZ71_001132"
     CDS             complement(2596..3207)
                     /gene="rpsD"
                     /locus_tag="MBZ71_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBT4429099.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            3469..4320
                     /locus_tag="MBZ71_001133"
     CDS             3469..4320
                     /locus_tag="MBZ71_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBT4429100.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            4348..4983
                     /locus_tag="MBZ71_001134"
     CDS             4348..4983
                     /locus_tag="MBZ71_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBT4429101.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            5003..5776
                     /locus_tag="MBZ71_001135"
     CDS             5003..5776
                     /locus_tag="MBZ71_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4429102.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            6184..6981
                     /locus_tag="MBZ71_001136"
     CDS             6184..6981
                     /locus_tag="MBZ71_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBT4429103.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            6959..7372
                     /locus_tag="MBZ71_001137"
     CDS             6959..7372
                     /locus_tag="MBZ71_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBT4429104.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            7356..10001
                     /gene="mutS"
                     /locus_tag="MBZ71_001138"
     CDS             7356..10001
                     /gene="mutS"
                     /locus_tag="MBZ71_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBT4429105.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            10019..12127
                     /gene="mutL"
                     /locus_tag="MBZ71_001139"
     CDS             10019..12127
                     /gene="mutL"
                     /locus_tag="MBZ71_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBT4429106.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            12149..12709
                     /gene="maf"
                     /locus_tag="MBZ71_001140"
     CDS             12149..12709
                     /gene="maf"
                     /locus_tag="MBZ71_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBT4429107.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(12794..14782)
                     /gene="treP"
                     /locus_tag="MBZ71_001141"
     CDS             complement(12794..14782)
                     /gene="treP"
                     /locus_tag="MBZ71_001141"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBT4429108.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            15005..15718
                     /gene="treR"
                     /locus_tag="MBZ71_001142"
     CDS             15005..15718
                     /gene="treR"
                     /locus_tag="MBZ71_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBT4429109.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            15795..16241
                     /gene="msrB"
                     /locus_tag="MBZ71_001143"
     CDS             15795..16241
                     /gene="msrB"
                     /locus_tag="MBZ71_001143"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBT4429110.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            16411..17013
                     /gene="ruvA"
                     /locus_tag="MBZ71_001144"
     CDS             16411..17013
                     /gene="ruvA"
                     /locus_tag="MBZ71_001144"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBT4429111.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            17026..18027
                     /gene="ruvB"
                     /locus_tag="MBZ71_001145"
     CDS             17026..18027
                     /gene="ruvB"
                     /locus_tag="MBZ71_001145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBT4429112.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            18056..18634
                     /locus_tag="MBZ71_001146"
     CDS             18056..18634
                     /locus_tag="MBZ71_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4429113.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            18686..19168
                     /locus_tag="MBZ71_001147"
     CDS             18686..19168
                     /locus_tag="MBZ71_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBT4429114.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            19285..19659
                     /locus_tag="MBZ71_001148"
     CDS             19285..19659
                     /locus_tag="MBZ71_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429115.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         6572 a         3592 c         4363 g         5451 t
ORIGIN      
        1 ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact ttatgttgtt
       61 ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt gatcaaaaca
      121 agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc agatgattgg
      181 gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta tttatcacag
      241 gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa gatgcttttg
      301 tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga tttcctgaaa
      361 cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa gcggtatcga
      421 aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg atattggatg
      481 cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat gagacgaatg
      541 tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc caaaaaagcg
      601 gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt gggatcgctt
      661 atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg gattacgcag
      721 gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca atgaatgcgg
      781 ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca ccagtcaagc
      841 gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct cctcaagcaa
      901 gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa taaccttgtg
      961 tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga tgataaaaaa
     1021 agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat tctttttaat
     1081 aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa cgatattcaa
     1141 aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc tatttttatt
     1201 tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg acacttcagt
     1261 tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta tcttgcaaag
     1321 gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac aacgacagat
     1381 gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt tttgtctgtc
     1441 tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct gtccttgtat
     1501 aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat tgcttctcct
     1561 cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt gcttatcaac
     1621 tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac agcatgcgta
     1681 tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca actacctttt
     1741 ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt cggtgggata
     1801 attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt ttaatttaaa
     1861 atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt ttttgattaa
     1921 tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata agctgctcca
     1981 gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa tgcagttgta
     2041 aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc agcaagagag
     2101 cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc gatcatccaa
     2161 ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg cccaaataaa
     2221 acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc aagtaaaaga
     2281 taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt taatccaata
     2341 gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg agaaaggaca
     2401 gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg gttcataacg
     2461 ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa caaagtgatt
     2521 actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg gtctttttca
     2581 agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag cttcgtcgat
     2641 ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca atttttcagc
     2701 gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt ttactgttac
     2761 gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt ggtatgaagg
     2821 gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt gacgtgcttg
     2881 acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt gttctagtaa
     2941 gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta cgaacaagtt
     3001 acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt cagttaattg
     3061 catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg gtgcgtaagg
     3121 gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac gagaaatttt
     3181 ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata agtttttgga
     3241 gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc ttcacctttg
     3301 cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg agttattatt
     3361 tttctgctgc attattttac acaaattaca gtatatgata gatcatccac gaatgtcaag
     3421 ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat gagagggtat
     3481 gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc ggtatcacca
     3541 ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga aaacagcttt
     3601 tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg aacggctgtg
     3661 gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa gattttatac
     3721 gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga attaggaaca
     3781 tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt aagtaaggca
     3841 gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt atgtggattt
     3901 ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat gttagaatca
     3961 atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat gtttgcgaat
     4021 atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt cggaaaagtg
     4081 atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca cgtgatagcg
     4141 aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga tggaatgaca
     4201 gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat tggtggcggt
     4261 ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta tgtggactaa
     4321 aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa aaatgaggaa
     4381 aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg agtacctaag
     4441 ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt atctatttct
     4501 gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta tcagtatgta
     4561 gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt agtcgtattg
     4621 ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag aaaagtatac
     4681 agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt gtttgaaaaa
     4741 aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta taaacagcaa
     4801 acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa ggaattcttt
     4861 atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc cgattgggta
     4921 aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag taagtattta
     4981 taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata ttgatggaac
     5041 gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg aaaaattacg
     5101 caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg cgcaagagat
     5161 cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg gttttttaga
     5221 ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt tcgttcaaga
     5281 agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca agcgttcttc
     5341 ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg tcttgcctga
     5401 attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat ttttcgatca
     5461 aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct ggcatgagaa
     5521 cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc acttagcaga
     5581 acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa acgaccgtga
     5641 aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag aagtacaagc
     5701 agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg cattgaaaga
     5761 gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta ataagcccag
     5821 ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg cagatgcaag
     5881 ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca agaaataaga
     5941 agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt tctgaagaag
     6001 tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt catagtccca
     6061 tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt ttgtttgacc
     6121 ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga agaggaattt
     6181 agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga gatgattact
     6241 gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt aaatggtgag
     6301 aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt gcaagacggc
     6361 gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt tgagtttgta
     6421 aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc tggacaaggg
     6481 atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca ggctcgttct
     6541 tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga agaagccaga
     6601 aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga aaaacaagca
     6661 atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac acacgttcag
     6721 acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt tgggatgaca
     6781 ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa gtttctgact
     6841 gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag cggctgtatc
     6901 ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca gatcaacgat
     6961 gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc cggaaattca
     7021 aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca ttcgctacaa
     7081 agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag aaaaaatcaa
     7141 acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag cagaaaaaga
     7201 agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc ttgttgtagc
     7261 ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa ctatgataca
     7321 agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact aaaaatactc
     7381 ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt ttgttctatc
     7441 gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca cagcttttgg
     7501 aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg tgcggtgtac
     7561 ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat aaagtagcga
     7621 tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc gaagtgatcc
     7681 agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa gacaacaatt
     7741 ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc gatttaagta
     7801 cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat gaagcctctg
     7861 cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt cgtgaacagc
     7921 taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa aacgcggaat
     7981 ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc ggcaaactat
     8041 tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa gcagtagaat
     8101 accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta gaattaagcc
     8161 aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat gaaacaaaaa
     8221 cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc caggcaaaac
     8281 aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt gaacgtatcg
     8341 atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt cgagtagctt
     8401 tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa caagtgccaa
     8461 tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg ctttcagaaa
     8521 tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat gatccgccgt
     8581 tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc gatatgtacc
     8641 gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa gagcgtcaag
     8701 aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat tatatagaaa
     8761 ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa caaactttag
     8821 caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa atcctcgaag
     8881 cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga gaagaagtca
     8941 aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta gatgtgctac
     9001 aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt gtcagtgaca
     9061 agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta ttaggacatc
     9121 aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg ctgattacag
     9181 gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact gttctgatgg
     9241 cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt gatcggattt
     9301 ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt atggtagaga
     9361 tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt ttgtttgacg
     9421 aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca atcatcgaat
     9481 atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat gaactgacgg
     9541 tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt gaaaaagacg
     9601 gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc tacggcatac
     9661 atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca gatatcctgc
     9721 atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta gaagagaaaa
     9781 ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat caagaccata
     9841 tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa ttatccgaaa
     9901 atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg acgccaatgg
     9961 atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc ggatatcgat
    10021 ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg gagaagtagt
    10081 ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg ccggcagtac
    10141 tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga ttgataacgg
    10201 agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga caagtaagat
    10261 ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg aagctttgcc
    10321 aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag aaaaacaagg
    10381 cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac cactgcgtca
    10441 aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct tgaaatacgt
    10501 gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac tggcattgag
    10561 tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg caactgctgg
    10621 aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg caaaaaaaat
    10681 gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt cattgcctga
    10741 ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt ttatcaagaa
    10801 cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg tcggtcgttt
    10861 ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa atgttcatcc
    10921 gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa ttagtaacgc
    10981 cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata atctacggtt
    11041 taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt tgtcctacgc
    11101 ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag agacaggtac
    11161 ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata agtctgtgga
    11221 aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag aaaaaacagc
    11281 gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa cagtggaaaa
    11341 atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg aagaacaagc
    11401 agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac tccatcctga
    11461 atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga aattagaaac
    11521 ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc acggaacgta
    11581 tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg cccaagagcg
    11641 cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc tgcaagaatt
    11701 gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa aagagcaaaa
    11761 acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca gctttattgt
    11821 acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc gggagatgat
    11881 cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag caacagcaat
    11941 catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag cacaagcacg
    12001 tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc atggccgtcc
    12061 agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa tccaagaccc
    12121 gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc aatcaccaag
    12181 aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg ctgatatcga
    12241 tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg ctgaagaaaa
    12301 agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg ccagtgacac
    12361 gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg aggctgaatt
    12421 catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg ttttaaaaac
    12481 aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc ctttgacaga
    12541 cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag gggcatacgg
    12601 catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt acagtatcgt
    12661 aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa caaaaaagcg
    12721 gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg aaaagattac
    12781 ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc gggcgaacaa
    12841 gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa gcaacaataa
    12901 tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca atcagggtct
    12961 ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc tttagttcaa
    13021 cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga ataccaatgg
    13081 catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag actttgccgt
    13141 ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact gggtcattta
    13201 cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct tttgttgcaa
    13261 ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt gcgccatttg
    13321 ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc ttattcaaaa
    13381 ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca atgatcattg
    13441 cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc aaaccgccta
    13501 ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct aatccagagc
    13561 cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac atagcaggtt
    13621 cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct tcttttttat
    13681 tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata tttgataaag
    13741 caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata tcgatggcat
    13801 tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg aatacggcac
    13861 taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag atccagcttc
    13921 cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt ggtaacaatg
    13981 aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg aagaagattt
    14041 ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct tgataaccaa
    14101 tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg gctgtggaag
    14161 ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa acgattccta
    14221 atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact ggaagaaaat
    14281 ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa aacttcgagt
    14341 gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca tttctgaatc
    14401 caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt tctgctaaaa
    14461 cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt ccttgttctt
    14521 tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta gaaacatcgt
    14581 ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg ctaggtatat
    14641 cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc atacgagtcg
    14701 cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac agcttctcgg
    14761 catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt ctatgcaagt
    14821 tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta ttaaaaagta
    14881 cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa aataggaaca
    14941 ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga gtgagggaaa
    15001 aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt tagcgaaaga
    15061 atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt atggtgtttc
    15121 acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata ttcaaaaaaa
    15181 acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta tttctggatt
    15241 gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga tcatccatac
    15301 gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg agatcggtgc
    15361 gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta tattagacac
    15421 cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac aaggatcgat
    15481 ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa aggagatcac
    15541 agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg acacttatgc
    15601 agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt attctgagtc
    15661 ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa aagtgtaaat
    15721 agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat aggatcacaa
    15781 agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg aaagaaaagt
    15841 tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca tttagcggga
    15901 aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt gaaccgttat
    15961 tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc aaaccaataa
    16021 cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga atagaagttc
    16081 gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca gtagatcaag
    16141 gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat gaaaagatgg
    16201 atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac acaaagagcg
    16261 gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga ttgctatttg
    16321 aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa taaagggaac
    16381 gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg tgtggttacc
    16441 tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca gatcgcacta
    16501 ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta tgtccatcag
    16561 gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga aaagcaactg
    16621 tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc aatcatggca
    16681 agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac ttatttgacg
    16741 aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct aaaaggaaaa
    16801 ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa cacaaaagac
    16861 gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag tgatcgagaa
    16921 atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga tgagtatcta
    16981 cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac agatgaatac
    17041 ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct tcgaccacag
    17101 ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat ttacatcgaa
    17161 gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc tcctggatta
    17221 ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat tcgtacgacg
    17281 agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga actggaagca
    17341 ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga agagatgctt
    17401 tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc aaccgcacat
    17461 ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg ggcaggtatg
    17521 ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata ttatgacgaa
    17581 caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga gatcattgaa
    17641 gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc taatcgcttg
    17701 ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga tcgtcctata
    17761 gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta tgttgaccag
    17821 aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt aagtacgctt
    17881 tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc gtatctgatc
    17941 caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta tgcctatgaa
    18001 cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa aatgaatgag
    18061 tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt gccaaacgca
    18121 atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca atcgacaaac
    18181 attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt gtgaggatgc
    18241 attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc aagcattgaa
    18301 aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc tttctgaccc
    18361 agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca agatttttga
    18421 acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg ctcgtttttt
    18481 ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg aagaaacacc
    18541 gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat attcttatcg
    18601 tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt tggaaaagca
    18661 ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt tgggaaatat
    18721 ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag aaggaaggga
    18781 agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa agacaccgca
    18841 ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata tggttgctcg
    18901 gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg atcaagaaaa
    18961 tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc atttgttttt
    19021 aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata ccaataactt
    19081 tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata tttggattga
    19141 tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg cgatgttaga
    19201 gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag taacactttc
    19261 ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat gaagaagctt
    19321 acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa tgacactgaa
    19381 gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca aaaagctgct
    19441 actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag caagtatgaa
    19501 acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat gatccgccta
    19561 caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa aataacaatc
    19621 ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga tagaaaagca
    19681 cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct ttaagctgat
    19741 atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc ggttgattag
    19801 ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta gccggggagc
    19861 tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt ggtatgctta
    19921 tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt gttaagtt
//
LOCUS       DAGCWU010000041        19401 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.041, whole genome shotgun sequence.
ACCESSION   DAGCWU010000041 DAGCWU010000000
VERSION     DAGCWU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.041"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            42..3569
                     /locus_tag="MBZ71_001149"
     CDS             42..3569
                     /locus_tag="MBZ71_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBT4429116.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3566..7288
                     /gene="addA"
                     /locus_tag="MBZ71_001150"
     CDS             3566..7288
                     /gene="addA"
                     /locus_tag="MBZ71_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBT4429117.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7357..7698
                     /locus_tag="MBZ71_001151"
     CDS             7357..7698
                     /locus_tag="MBZ71_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4429118.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8007..9050
                     /gene="pheS"
                     /locus_tag="MBZ71_001152"
     CDS             8007..9050
                     /gene="pheS"
                     /locus_tag="MBZ71_001152"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBT4429119.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9055..11475
                     /locus_tag="MBZ71_001153"
     CDS             9055..11475
                     /locus_tag="MBZ71_001153"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBT4429120.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11645..12655)
                     /gene="trpS"
                     /locus_tag="MBZ71_001154"
     CDS             complement(11645..12655)
                     /gene="trpS"
                     /locus_tag="MBZ71_001154"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBT4429121.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12909..13646
                     /locus_tag="MBZ71_001155"
     CDS             12909..13646
                     /locus_tag="MBZ71_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4429122.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13660..14475
                     /locus_tag="MBZ71_001156"
     CDS             13660..14475
                     /locus_tag="MBZ71_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBT4429123.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14488..15135
                     /locus_tag="MBZ71_001157"
     CDS             14488..15135
                     /locus_tag="MBZ71_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4429124.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15151..15807
                     /locus_tag="MBZ71_001158"
     CDS             15151..15807
                     /locus_tag="MBZ71_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4429125.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            15969..16790
                     /gene="racE"
                     /locus_tag="MBZ71_001159"
     CDS             15969..16790
                     /gene="racE"
                     /locus_tag="MBZ71_001159"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBT4429126.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16793..18148
                     /gene="rph"
                     /locus_tag="MBZ71_001160"
     CDS             16793..18148
                     /gene="rph"
                     /locus_tag="MBZ71_001160"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBT4429127.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18149..18667
                     /locus_tag="MBZ71_001161"
     CDS             18149..18667
                     /locus_tag="MBZ71_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBT4429128.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18774..19274
                     /locus_tag="MBZ71_001162"
     CDS             18774..19274
                     /locus_tag="MBZ71_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4429129.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6519 a         3339 c         4171 g         5372 t
ORIGIN      
        1 taataaagga gcaccgtatc atgtgttgaa ggaggaaaag catgtcttta caatttattc
       61 ttggaaacgg gaaaaaagat caccgtcaag tcttgatcga cgaagcatcc gcttggctcg
      121 accaggatgc aagtaaccaa gtatttttcc ttgttcccaa ctacagcaaa ttcgaacagg
      181 aacaagaaat tttagcggag atgcgaagaa gaagcggaaa aaaaactttc agcacaacaa
      241 acgttcaagt atttagtttt tatcgtctgg cttggtattt gctgcaacag acaactcttc
      301 ttacaggaaa tgaattatca gaatcaggat ctgcaatgat cttgcgacag attttagaaa
      361 aaaatgagga agaattaaca atctttcgcg gagaaatcaa taaaagaggg tttattcgtc
      421 aattacaaga actctacagt gaattgcgta caggaaatat tcaaccagaa gacctgatgc
      481 ttttatttgg tgattcacct aatgcagaag accagcaact taaaatgaaa gacttgaagc
      541 tgattttttc agcttatgac agcgaattga ctcaacgtgc actaacaaat gaagatgcac
      601 tatctatact agcaaattat atgcagacac aagatttcaa caacgtcaaa tttattgtca
      661 gtggtttttc tcgaatcaat ggtcaagagt atcaattact gcaggcaatg atggaaaagg
      721 ggcaattggt tgtcgattta ttgctggatc gtccttatac agcggatctt ccagaagtac
      781 ttacgctctt tcatgagact ggaaagcttt atttccgtct ttttcaaagt gcaagagaac
      841 ggaaagtccc tatattagtg gaccgatttg caccagagca gactcgtccg acagagcttc
      901 aactgctgca aacaatgtgg gaagagacat ctagccaaaa gaaagcggca gtacacaaaa
      961 tagggggaca agagcatctc catttgtggg aagctgaaaa tccaactgaa gaagtccgac
     1021 aattagctgt tgaaatcaga cgactagtca gtgaagaaaa ctaccgttat cgtgatatcc
     1081 aagtgctgac aaaaaatatg tctttatatg gaaatatcct tcggccgatc tttcaagaaa
     1141 tggcaattcc tttttatatc gatgaagaac aaatgatgga aaatcatccg ctgatcgaat
     1201 ggatcaacag tctgttcgct cttgaccgct attcttatcg gctaagtgat attatgcgtt
     1261 ttttcaaaac tgaattattt ctgtcgacgg aagcagaaac cgaacttgat gaatggaaac
     1321 aagagcgaga tgaattcagg aaaaaactgg acatcactga aaatgtagca cttgcttaca
     1381 attatcaagg aacttattgg actcgtgaaa aagactggca gtttgtggcg tatgattttg
     1441 aagcagaaca gattgaagac gtacagtatt tggaagaaca gtcaaatacg attagacgat
     1501 tatttcagaa agctgtacca gtatttttga aaaaaataaa gaaaagcaaa acaggacaag
     1561 aggccgcaaa tgttttttat caatttttga tccaaagtgg cgtacagcaa caattattgt
     1621 tttggcgtaa tcaggaagta gacagaggaa acttagaagc ggcaagaaac catgagcaga
     1681 catggggagc gctgattgat ctgttggatg aatatgtagt aatttatggt gaatcggctt
     1741 ttgactggtc actgttccaa gaaatcatta caagtggtct agaaaatcta agttatggta
     1801 aaatccctac cgcaatcgac caagtgcgaa tcaatcgttt ggaattagtt cgaccaaatc
     1861 aagctgccgt cacttttgca ttagggttga atgatcaagt gtttccagac cgaaaagaga
     1921 caaaaggatt gttgtccagt gaagaacgag aagtattaaa tcaggccttg ccagatggac
     1981 agtttttatt cgacccatca aaggaaagta tctcttttga gcctttccaa gcctatcttg
     2041 tttttctttc cggaacagat cgattatatc ttagttatgc acaaagctat gacacagatg
     2101 gttcgctgaa gatgtctcct tatttgcgta gaatcacaga atatttatct gttcccgtcg
     2161 agagaaaaga gcatttgacg atcgaaagcg accctaattg ttatgtaggg acttacagaa
     2221 gcggaatcaa tacagtcaat aggatttatc gattagcttt agatgaaaaa aaacctgtac
     2281 caaagcactg gcaaaagctc aaagaattag tactgaattc gtcatggcga aattttgctt
     2341 tacagatttt tgaaagtcag gaacatttaa atgttcctgt ttcgatgcca gaaaaaatgg
     2401 cggaagagct ttatggaaaa gagatttata cttcaatttc acgtatggag aatttttata
     2461 attgtgaata caaatacttc atgcaatttg gtttgaaatt gaaagaacga actgtttatg
     2521 gtctaactcc agcagcaact ggtgattttt atcatgaatc attggatcgt ttcttcaagc
     2581 tactgttttc aaaccaatta tccttagtga atatgtccga tgcgcagcgt agagacttta
     2641 ctgaaaaagt gctgcaggaa gtattcggag aacttcggtt tgagatattg gatagttctg
     2701 caagaatgaa ctatatccgc taccagttag gacagacgat tcaaaaagtg acatgggctt
     2761 tgcaaaaaca gagcaaaaat acaggcatgc atcctcttca gacagagatt ctatttggtc
     2821 agattgctgg agcaaaaggt atcacaggtc tggaattacc gttgagtaat ggtggaaaac
     2881 tccatgttcg agggaaaatc gatcgaatcg atgtagcttc tgaacaagaa gatacttggc
     2941 taagcgtcgt tgattacaaa tctagcggac gttcattcga tgtcacagaa gcttattatg
     3001 gcatggctat gcaattattg acgtatctgg acgtagcatt gatggatgcg gtacgtttga
     3061 ctggaaaggc ggctgttaaa ccagcaggat catattattt acatgttcat aatcctgtat
     3121 tgacggagac agatgatgta gagaagaata cattgaaaaa atttagttat gatggcgttt
     3181 ttgtcgatga tcccgctctt ttagaagtac tagatcattc actggaagca aaagaacaat
     3241 cactgatcta tccagtgaaa aaaaatgcga aagatgaata ccaaaaaggt agtttcagta
     3301 aagacaaatt tttcacggaa gaagaactgc aactattcat ggcgcataat cgggaaaata
     3361 tgcggttagc tggagatgcg attacttccg gagaaatcaa actaaatcca gcttataaag
     3421 ataaacagcg gattgcctgc gaattttgtc cattcagaag catctgtacg tttgatgtga
     3481 tgctgaaaga aaataattac caccgtctgg agaaaataga taaggaagaa gcactgcgta
     3541 gaatattgaa aaggagtgaa gaagagtgaa tcaaattcct ttaaaaccgc aaaatgaacg
     3601 attcacagat gatcagtggc aagcgatttt tgatcaagga gataatctat tagtctctgc
     3661 gtctgccgga tcaggtaaaa ctacagtgct tgttcgaaga gtgattgaaa aactaaaaat
     3721 gggttttgat atcgatgaat tattgattgt gacatttact gaagctgccg caagagaaat
     3781 gaaagaaaga attcaagagg cattacaaga atccgtcaat agcgaaagtg accctgtacg
     3841 tagacagcat tttactaagc agttagtttt attgcccaca gcgaatatct caaccttaca
     3901 tgctttttgc ctgacagtta ttcgacgtta ttattactta attgatattg atccagtctt
     3961 tcgcatgtta acagatgaaa cagaaacgat cttgatgaag gaggatgtgt gggacgagct
     4021 gagagaagca ctttatgcag aaaatgatga acgatttttc caattaacaa tgaatttttc
     4081 taatgatcgt tcagatgacg gcttgacgaa tctggtgttt tctctttatg agtttgcccg
     4141 tgccaaccct gacccccaaa aatggctaga acagttaagc gataattatc gtttgcctga
     4201 ggggctagcg aaatctcgat tatatcaaaa acaaatccgt cctttagttt tggcagatat
     4261 ataccaatgt gtacagttat atgaacagat gactcagtta gcacaaggtg aaggattaga
     4321 aaaaatgcat gagcaagtag ctggagaaca acagcagatc aagaatatct atgaagcgtt
     4381 ctcgcaagat cggttagaag aagcttatgc tggtttggaa caactaactt tttcaacttt
     4441 taagagcagt cggaaagcag aattgaaaga aatttccaat gaagtcaagg gcatgaggga
     4501 taaagcaaaa aaactgattc agcagataag taaaagttat tttcctgttt ccccaagcca
     4561 aatggaagaa ttaacggaca aagcactacc gttagtcgaa gaaatgacaa aagtcacgca
     4621 aagcttcatg gacggtttca gtatgcgtaa acgggaaaaa ggtgtgctgg atttcaatga
     4681 tctggaacat ttagcgttgc agattctcac agaaaaaacg aaagatgtat ggctgccaag
     4741 tgaagcttca aaacattatc gaaaaaaatt caaagaagta atggtagatg aatatcaaga
     4801 cgtgaaccaa ttacaagaag ccattttata ctggctaaga gaacccgatg atacaaaagg
     4861 aaatatgttc atggttgggg atgtcaaaca atccatctat tctttccgtt tagcagatcc
     4921 tagtttattt atcgggaaat acgagaattt ctcaaagaaa gaaggcggac gtcgaatcgt
     4981 tttggctgag aacttccgct ctagaaaaga agtcctttct tttacgaatc ttatttttga
     5041 acaactaatg gatccagcag tgggtcaaat caattatgac gaagcagcca aactgatcca
     5101 aggattttca gactttccag agaatgaaca gtttgagccg gaaatcatga tctatgaaaa
     5161 agaacaggaa gaatcagaga ttgagattcc gacggatgat atactggaag acaaaacaga
     5221 aggcgaactt tttatgacag gactaaaaat tcgtcaatta attgattcct cctttatgat
     5281 ttatgataaa aaatcaaaaa aaagtcgtcc gatagagtac aaagacattg ttctcttgac
     5341 acctacaaag aagaacaact taacgatttt agagattttc aaaacattgg atattccatt
     5401 agagatgaat gatgctcaaa actattttca agcgacagaa attcgcacga tgatctcgct
     5461 gctacaatta atcgataatc cctatcaaga tattccgctt gcggctgtat tgcgctcacc
     5521 gatcgtgggt ctgattgaac cagaattagc cagcattcga ttagctaacc gtgcacatac
     5581 ttactatgat gctgtgcttg cttatcaagc atcaaacgaa gatgaattag cagctaagct
     5641 ggaacatttt ggcaagcaac tagagcattg gcgagaactt gctcgccgtt cttcgatcac
     5701 tgacttgtta tgggatatct attatgagac gggatatcta gagtatgtgg tcggtctgcc
     5761 agcaggggca cagagacaag ccaatcttta tgcattggtt gatcgtgcca aagcttacga
     5821 gcaaagttct ttcagaggat tataccagtt cgtgcgtttc attgaaaaaa tgcaagaaaa
     5881 agataaagac ttagcagaac cagtcatttc aatagaagat aatgctgttc gcgttatgac
     5941 gatccatgca agtaaaggct tggaattccc agttgtattt ttgttagata tgacaaagga
     6001 gttcaactta caagacttgc gcaatcggta tgcttttgaa gaaaaacttg gagcaggaat
     6061 tcgctatatg gatcctgaaa cacgagtgtt atatgatacg ttaccgtttc aagccatcaa
     6121 attggcaaag caaaacaaat tgttgtcgga agaaatgcgt aaattgtatg taggattgac
     6181 tcgggcagaa caaaagcttt tcattgtcgg ttcctataaa aataaagagc aaatgatcca
     6241 aacgtggtct gaagcagctg atcatgagga gttagttttt gatcctgcgc tacgtttgaa
     6301 gggcagaagc agcttgatga attggattgg ttacggattg atccgtcatc ctgaaatgca
     6361 gaaatactta gaggaagaaa tttcgacttc tcttcttcaa cattcaaatg cacagttttc
     6421 tatttcatgg atgaatcagc aatcgatcat tgaacaaaga cagttgcttg ctgaaaaaga
     6481 actggttaac ttagatcagc agatgaaaga agatgaaact ttattagcag attcgttaca
     6541 aaaacgtctg gcatatgaat atccttatca agcttcttca caaaccacaa gttatcaatc
     6601 tgtatctgaa atcaaacgat tattcgaaga tccagatgat acacaagaat cacgtttgac
     6661 tctggaaagc agtcagaaca aagcagcatc ccgtcaattt cgttatacgc aagaacaact
     6721 agcagaacca aaatttttgc aaaaagatcg gcaagtgtca gcagctgctg ttggaactgc
     6781 tacgcatgct ttgttacaat tactgccatt agagatgcca actacagaga gtatccatca
     6841 aaaattacag gaattggtaa ggaaacggtt agtagacgaa aaagtcgcga aaaaagtaga
     6901 tgtttcttct attatatggt tctttcaaac agaacttggt caacagctga ttgccaacaa
     6961 agaaaacgta aaacgagaac aacctttttc catgctgctt cctgctgacg aagtatttca
     7021 agactatcca aatcaagaag atgaactatt aatccacgga atcgtcgatg gatacctaga
     7081 agaaaaagat catctcaata tctatgattt taaaacagat tttattttac atccggatga
     7141 tccagcagaa atagatgcaa tcgtccaaaa atatcaagga cagctaagac tttaccaaca
     7201 agcaatgtca gaagctttga ataagccagt agaaaatgtt ttcctaattt tgcttcgagt
     7261 gaaacaaatt ataaacatta acaaataatt ataattgagc aaacttgact tatgttttgt
     7321 aaggattatg ctatactctg ggaagaggtg agtatgatgg agctaacaca gacagaattc
     7381 gttttatgcc ctaaatttga aaaaacattc tctattttag gaaaaaaatg gaacggattg
     7441 attatcgatg tactactcga aaacggtccg caacgctttg ttgagcttgc ggcaaaaatc
     7501 ccgatggtca gtgatcgagt attagtcgaa cgcttgaagg aattagaaaa agaaggaata
     7561 gtcactcgaa actttgcctg tagcgaaaag agccgttcag aatatctctt gactgaaaaa
     7621 ggagcagact tgcaaaaagc gatgcgtcaa atacaagttt ggagcgaaaa atggatcaca
     7681 gaggaagaat gcggttgatt ttgtaaaaaa aatcttgtaa actatagtcc atacgaataa
     7741 gtaaagaacg atgacgaaaa agagtagttt ggctaaacca gttcagggag atctgtcgca
     7801 gactgaaagc agatcctgtg tttgaaaaat gaagttcact ttccgagttc gtctgataaa
     7861 atcagaccgg caattgtcgt taacgaagta agagtgcggt gtaagtcatt tatccgcaaa
     7921 ttaggatggt aacgcgaaga cctcgttcct taacaggaat gaggtctttt tgttttgtat
     7981 ttggaattta aaggaggaac aagcacatgt ctctacaagc acaattagag agcttgaaag
     8041 aagcaacgct cgtcaaaatc acagcggcac aagatttaaa tgaattaaat caaatacgtg
     8101 ttgaaacgtt aggaaaaaaa ggaccgatca cagaagtatt acgtgggatg aaagatctaa
     8161 gtccagaaga gcgaccaaaa gtcggaagtt tcgcaaatga gatccgagat cttttgacag
     8221 aagagatcga gcgaaaaaaa caagtattag aagaagaagc gttaaatgca gctttggcaa
     8281 aagaaacaat agacgtgaca ctacctggta gaaaagtaaa acatggtaca cgtcatatcg
     8341 tgacacaaat catggaagaa attgaagata tttttgtggg tatgggctat cagatcgtag
     8401 aaggatatga agtagaatct gatcattaca atttcgaacg gatgaatctg ccaaaagatc
     8461 atccagcaag ggacatgcaa gatactttct atatttcaga cgagatattg attcggacac
     8521 atacttcacc agtccaagca cggacaatgg aaaaacatga tttctctaag ggtgctctac
     8581 gaatgatctc gccagggaaa gtcttccgca gagatacaga tgatgcgacc cacagtcatc
     8641 agttccatca aattgaaggg ctagttgttg ataaaaacat cacaatgggc gaccttaaag
     8701 gaacattaga agtcgtaatg aaaaaaatgt ttggggaaga tcgtgaaatt cgtttgcgtc
     8761 caagttattt cccatttacg gaaccatcag tagaagtaga tgtcagctgt ttcaaatgtg
     8821 gtggtgccgg ttgtaacgta tgtaaataca ctggatggat cgaaatttta ggagctggca
     8881 tggtacaccc gaatgtgttg aagatgtcag gaatcgatcc agaagaatat tcaggctttg
     8941 ctttcggcct tggaccagac cgcgtagcta tgttgcggta tggagtgaat gatatccgta
     9001 atttctatca aaatgactta cgtttcttaa gtcagttcaa gggggagtaa gaaaatgtta
     9061 gtttcatata aatggctcaa tcgttatgta gatttatcga atgtaacacc aaaagaatta
     9121 gcggataaga tgtctgtgac aggaatcgaa gtagagggag taacggttcc agaagaaggc
     9181 ttgaaaaaaa tcgttgtcgg tgaagtcaag gaatgcgtac cacatccaga ttctgatcac
     9241 ctttctattt gtcaagtcga tattggcgaa gacgaactgt ctcaaatcgt ttgtggagcg
     9301 cctaatgtga aggttggaat caaagtgatt gttgcacttc ctaactcacg tatcgctggt
     9361 aatgtaaaaa tcaaaaaagg aaaaatgcgt ggccaagtat caaatggcat gatttgctca
     9421 ttacaagaaa ttggctattc agatagtgtt ataccgaaag aatacgctga aggtatctat
     9481 tatatgccac aagaagctgt aaatggcgat ccagtttttc cttatttgga tatggatgat
     9541 tcgattattg aattatctat cacaccgaat cgtgcggatg cattgtcaat gcgtggcgta
     9601 gcttatgaag ttggggcaat ttatcgtcag actcctacat tcgatgacga aaagctggta
     9661 gaatcctcac gtcctgctag cgataaaatt caagtggaag ttgaagataa agaagcagtt
     9721 cctgcttatc aaatcagaat cattgaagga gtaagaatcc agccaagtcc tcaatggctg
     9781 caaaatcttt tgatgaacga aggcattcgt ccaatcaaca atgtagtaga tgtgacgaac
     9841 tatattttgc ttttattcgg acaaccacta catgcatttg actacgacaa attgaaatca
     9901 aaagagatct ttgtccgccg tgcaaaagaa aatgaaacat tagtaacact agatggagaa
     9961 tctcgggaat tgacaactga caatctcgtc attactaacg gcaaagaacc agttgcttta
    10021 gctggtgtca tgggagggtt agactcagag atcacgaatg aaacaacaac agttgcacta
    10081 gaatctgctt tatttgatcc aatttcgatt cgtcgtacag caaaacaatt taatttgcgt
    10141 agtgaatcct ctgcacgttt tgaaaaagga atcaaccatg cgactgttgg agaagccagt
    10201 gaggttgctg cagcaatgat cgtttctctt gcgggtgggg aagttctaca aggcgcggtc
    10261 aaaggctcag agatcaaagc agaagacgta tcagtgaaga tttcacttga acgcatcaat
    10321 caatatttag gaacatctct tgtcgttaaa gaagtaaatg agatttttga agcacttgga
    10381 tttagctatg aagaaaagga gtatgtttat tccgtagtag taccaccaag acgctgggat
    10441 atccaaatcg aagcagatat tatcgaagaa gttgctcgta tttatggata cgaccagttg
    10501 ccatctacac ttccaagtgg agaaacggtt gctggaagct tgtcaaaaga acaagaaact
    10561 actcgtaaaa ttcgtacaat ctttgaagga agcggattga gcgaagcaat cagttatgca
    10621 ttgacaacag aagaaaaatc aagacagttt gttataaatg aaagtaatat tacacgtttg
    10681 gactggccaa tgagcgaaga acgttctgta ttacgaatga atttgatcag cggactatta
    10741 gatgatatca gttataacac tgcccgaaaa aatacagaga ttgctttata tgaaattggt
    10801 cgggtgttct tccaagaaaa tgatccacgt acacatctgc cgcaagaagt aaaacatgca
    10861 gcaatcgcta tttctggtat ttgggaagaa aaagattggc agacgaaagc tcaaaatgta
    10921 gactttttca ccatcaaggg tcttttagaa aacttatttg aacaactagg catcactgaa
    10981 gatgtccatt atcaaacagt gacagaaatg aaggaaatgc atcctggaag aactgctgcg
    11041 atttaccttg gcgaaaaatt tattggtttt gtcggtcaag tgcatccaac gattacaaaa
    11101 agttatgata ttccagaaac atatgttgct gagtttgatt tatctgctat agtggaagct
    11161 gctcaaaaag ggatcgtttt cgaagcagtg acaaaattcc cagcagtcag ccgggacatc
    11221 gctttgcttg taaaagaaac tgtgacaaat caggaattaa cagaagttat ccgttctgct
    11281 gccggaagat ttttaacaga tatccaatta tttgatgtct atcaaggaga aaacattgaa
    11341 aaaggacata aatccatggc gtatagcctg acattcgtca acccagaagc aacattgaca
    11401 gatgaagaaa tcaataaagc aatggaaaaa gtcgaaaaag cgttggtaga aaacctagag
    11461 gcaagtattc gctaagaagg aaaatatagt aagtaacgat caagtagctt ttgaagacca
    11521 tacatttgaa aaaaagatgt gacacaactt cgtcacatct ctttttttga ttttaaaaaa
    11581 ggaatagcac agagacgaga taaaaaaaca ggcctttaca gcctgctttt ttagagtaaa
    11641 tgaattaatg gaagtaattg attcccattg ctgattttac ttcagaaagt gtttgtgcag
    11701 ctactttagc agcttcttca ctacctttac gtaagatttc cataacagct gaaggatctt
    11761 tttccaattc ttctcggcgc gcacggatcg gtgcaaattc agcttccaat acatcaatga
    11821 ggtatcgttt gatctttaca tcaccaagtc cgccgcggcg gtaatgagct ttcatttctt
    11881 caattgcttc tttatctgtt ccgaatacat ccaagtaggt gaagaccata ttcccttcta
    11941 cttgtccagg atcttgtaca tggatgtggt ttggatcagt atacatactc atgacttttt
    12001 tctgtaagac atcggcagga tcggagatat aaataccgtt tcctaacgat ttactcattt
    12061 tgccatttcc atcgattcca ggtaatctac ccattccttt tggtgggaat acgccttttg
    12121 gttccaccaa gacttcacca tatgtatgat tgaagctttg tacgatttcc tgtgtctgct
    12181 ctagcattgg tttttgatcc tcaccaacag gtacaagatt cgctttgaat gcagtgatat
    12241 ctgctgcttg agaaacagga tagataaaga atcctgtagg cacgctttca ccgaattttt
    12301 tttgttcgat ttctgttttg acagtcggat tgcgccgaac gcgtcctacg ctgactaagt
    12361 tcaaataata catggttaat tcagctaatt ccggaatttg tgattggata aataaagtag
    12421 attttgcagg atctaatccc acggctaaat agtctaaagc aacttgcaag acatttgatg
    12481 aaaccttctc aggattttta gcattatctg tcagtgcttg catgtcagca atcatgacaa
    12541 acagttggtt gtcttcatct gcctgcatag ccactcgggt ttttagagaa ccgacgtaat
    12601 gccctaaatg cagcttccca gttggtcgat ctcccgttaa aataatattt ttcaaaaaag
    12661 acacctcttt acgatttgta agtctgagca cttaatatct taccgttttt cctgaaaaga
    12721 aacaacagtt cctaaggaaa aaacaaggaa agaggataaa ataactaata gattagaaat
    12781 ggaagaaata tttatgggag aataaacgtt ctaagaaact ttacaaatta ctaaactatc
    12841 agatataatg tattttatta tacatcttta aaagaaatct tactaaataa taatgggagt
    12901 gtaattctat ggcaatgatt aaatttgatc acgtggataa atattatggc aagttccacg
    12961 cattaaaaaa tatcaatctc gaattcgaga aaggagaagt tgtcgttgtc attggtcctt
    13021 ctggttcagg aaagagcacg atgcttcgct gtatcaatgg attagagacg atctcttcag
    13081 gtaagctgct gattaatgat actgacttgc atgataaaaa gacaaaactg acagaagtcc
    13141 gaaaaaacat cggcatggtt ttccaacatt tcaatttgta cccaaataaa actgttctgg
    13201 aaaacatcaa attagcacca atcaaagtgt tgaagcaaga ccaggcaact gcagtcaaaa
    13261 acgcggagaa atttttgaaa actgtcaata tgttagataa gaaagattct tatccttcca
    13321 tgttatccgg gggacaacag caacgggtgg caatcgccag aggtttagcg atgaatcctg
    13381 agatgctgct gtttgatgaa cctacttctg ctttggatcc ggaaatgatt ggtgatgtat
    13441 tagacgttat gaaaaaattg gctcatgacg gtatgtcaat gattgtcgtg acacatgaaa
    13501 tgggctttgc caaagaagtg gcggatcgtg ttatcttcat ggctgatgga gaagtattgg
    13561 aagacagcag aaatgtgaga gactttttcg aaaatccaaa tgaagtccga gcacagcaat
    13621 ttatcagtaa agttatcaat cattgatagg aggaaaaaga tgcgcaggtt aaaaaaaata
    13681 cgcctgattt tccttttagg cattgctttt cttgcactag ccggatgtaa aggaaatggg
    13741 ttggcaaatg aagatatttt agagcgaagc aaatctacca atgaaatcat ctggggtgtc
    13801 aagtatgata ctcgattatt tggaatgatg gatattgaga gcagaactgt acaaggattt
    13861 gatgtagata ttgcaaaagc aatcactaaa aagatccttg gtgacaatgg aaaaacagaa
    13921 tttgttgaag tcacttctaa aactcggatt ccattattga aaaacggaaa tatcgatgca
    13981 attattgcaa cgatgacgat cacagatgaa cgaaaaaaac aggttgactt ttcagatgtc
    14041 tatttcgatg ctggccaagc tttgttggta aagaaaggaa gccaaatcaa aagtgtagat
    14101 gatttgaatg cttctacaac ggttctagca gtcaaaggtt ctacatcggc tgcgaatatc
    14161 cgacagcatg caccagatgc aaaaatttta gaattagaaa actatgcaga agcatttact
    14221 gctttacaat ctggtcaggg agacgcgatg actacggata atgcgatttt gttagggatt
    14281 gcagatgaaa atccagagta tgagttagtt ggtggaacgt ttactaatga gccttacggg
    14341 attgcgatca acaaagggca agaaaacttc ttgaaagctg ttaaccaagc gctagaggaa
    14401 atgcatgcag atggcacata cgataaaatc tatcaaaaat ggtttccaaa tgaaactgaa
    14461 ggaaaagtag agtaaggggg agaggcaatg agcgaattga tacaaactta tggaccaatc
    14521 tttgtcgacg ggttcaagat cacattgttt tcgagtgtct tagcacttgt gtttagtttg
    14581 attatcggaa cgttaatggc aatcttccaa ttatcaaaga ataaagtagt gagcgcctta
    14641 gcaaaggcat atgtagaatt ttttagaaac attccattat taatcatcgt gatgtttttt
    14701 tatgttgtcg taccgattta ttggtttaat ctagatggat tccaagcagg tacgattgga
    14761 ttaacgatct atacatctgc ttttattgct gaaacagtac gttcagggat tcaaaccgta
    14821 ccaaaaggtc aaacagaagc aggactatct tctggtttta cctattcaga aatcatgcgt
    14881 tatattgttt taccgcaagc attcaaaatc gttatcccgc cattaggcaa ccaatttatc
    14941 aacctcgtaa aaaactcatc tattttagca atcgtagctg gtttagattt gatgtatcaa
    15001 ggagacttga tcgctagtga aacattcaat accttcgata cgtatatcat cgttgggtta
    15061 ttttacttag ttatcacgct accgttatct tacttaatgg tttatcttga gaaaaaatgg
    15121 gcggttcgtt catagaagga ggggaaatac atggatttta gcggcgcttt ttcatggatg
    15181 aatatccgtt ttttattgga aggattaaag gttactgttg aagtttcatt gttttcgatc
    15241 atttttagtt tcttgatcgg tggtctgaca ggtgtgattc gttttgcgaa tatccctttc
    15301 ttttccaaaa tccttggtgt agtgattgat attatacgga atcttccatt attattgatc
    15361 attttcttca cttactttgc cttacctcaa atcggcattc agatgaacat cttctggtcc
    15421 gctgtcgcag cattaacgat ttttgagtca gctatgttgt cggaaatttt ccgagctgga
    15481 ctcaatgctg ttccaaaagg acagatggaa gcaggattat caacgggact gacttatgta
    15541 gaatcgatgc gaacgatcgt gttaccgcaa gcatttaaat caatgatccc ggcaattgtg
    15601 agccaattga tttcactgat caaagatact tcattagcag taatcatttc tttacctgaa
    15661 ctttctcatc aggcacgtat tatttatgga cagaacacca attatgtgct tccgatgttc
    15721 atcatcatga cacttatgta cttcgtggta tgttacgcgc tttctctgct ttccggttac
    15781 ttggaaaaga gacggtacag ttattaatag atgattcttt tagcggttgt ggcaaactaa
    15841 agtcacaacc gcttttcctc tttccattcg aaaaaaagag atttcgattg ttttaagcaa
    15901 gtaagagcac ttgaaaattc acgctaaaaa gtacacaata gggaggatag aatttattga
    15961 aaaaaaggat gatacgattg acagataatc gccctatcgg atttattgat tcaggtgtcg
    16021 gcggcttgac tgtagtaaaa gaagccctga aacaattacc gaatgaaaat attttatttg
    16081 taggagacac agcacgctgc ccatatggcc ctagacccgc ggaacaggta atccagtata
    16141 cttgggaaat gacggattat ctggtggagc aaggaatcaa gatgctggtg atcgcctgca
    16201 ataccgcaac tgcggtggct ttagaagaaa tcaaagctgc tctttctatt ccagtcatcg
    16261 gtgtgatcct tcccggtact agagcggcag taaaaaaaac acaaaataaa caagttggca
    16321 ttatcggtac gattggtacg gtaaaaagtc aagcttatga aaaagcactg aaagagaaag
    16381 taccagaatt gactgtgaca agtcttgctt gtcccaaatt tgtttcagtt gtcgaaagta
    16441 atgaatacca ttcatcggtg gcgaaaaaaa ttgtggcaga aacattagct cctttaacca
    16501 ctaaaaaaat cgatacattg attttgggat gcacccatta tccattatta cgccccatca
    16561 ttcaaaatgt aatgggagaa aatgttcaac tgatcgattc tggagcagaa acagtaggtg
    16621 aagtatctat gctgttagat tatttcaatt tgagcaattc accgcaaaat ggtcggacat
    16681 tatgccagtt ttatacaact ggctctgcca aacttttcga ggaaatagct gaagactggc
    16741 ttggaatcgg acacttaaat gtagaacata tcgaattggg aggaaaataa ggatgcgtca
    16801 tgacgggaga aaagcccaag agcttcgcaa actggagctt caaacaaatg ttttcaaata
    16861 tccagaagga tcggtagtga tccgttttgg tgatacaaca gttatttgtt ctgcaacaat
    16921 agaaaattct gttccccctt ttttgagaga aacaggaaca ggatgggtga cagcagaata
    16981 tagtatgctg cctcgagcaa cgagtacgag aaatcgtaga gaaagcagca aagggaaact
    17041 ttctggtaga acaatggaaa tccagcgtct tattggtcgt tccttgcgtg cagtagtaga
    17101 cttggaaaaa ttaggagaac gtagtatcat cgtagactgc gatgtgatcc aagcggatgg
    17161 cggtactaga acagcaagta tcactggggc atttgtggca ttgaaattag ccattgaaaa
    17221 acttttaaga gaaaaagaat tgtcagaaga tccaataaaa gaacatcttg ctgcagttag
    17281 cgtaggaatc ttaccagatg ggacttgcgt cacagatttg gattaccaag aagattccgc
    17341 agcacttgta gacatgaatc ttgtaatgac agaatctgga aaattcgtgg agattcaagg
    17401 aactggagaa gaagcaacgt ttgatggtga acagttgaat gaaatgctct tttttggaaa
    17461 aaacgcgatt gaagatttga tcaaagagca aaaacatgct ttgctaacag agtttgctca
    17521 aaatgatgag cggatagaag aaacaaaaac aatcatcatt gcgacaagaa atccaggaaa
    17581 agcagaagag tttcgaaata tgttcaaaga agcgggatat catgtaaaaa cattacttga
    17641 ctatcctgaa ttgccagatg tggaagaaac tggcagcaca tttgaagaaa atgcccgatt
    17701 aaaagcagaa acgattgcac aattgcttga tcagccagtc ttagcagatg attcaggttt
    17761 gaaagtggat gctttaggcg gtatgccagg aatctattct gcacgttttg caggggaaca
    17821 aaaaagtgat gcggggaata acgccaaatt attatatgaa ttgacagatg tgccggatga
    17881 aaaaagaaca gcacaattcc attgtacgtt agtattcgcg gcacccaaaa aagacagcct
    17941 tgtagtagaa gcggaatggc ccggtcgagt agcccgtata ccatctggcg aaaatggctt
    18001 tgggtatgat cctttattta tcccagaagg taagaaacaa acagcagcag aattatcgag
    18061 cgaagaaaaa aacaagatca gtcatcgggc tcaagcaatg aaaaaattga gtgccgagtg
    18121 gaaacaatgg ctggaaggag agagatagat gcggtattta gtagtaagtg ataatcatgg
    18181 agacagagat atcctcgttg acttagtaga tcgttatcgt tctgaagtgg atatcttttt
    18241 ccattgtggt gattcggaat tagaagcttc tgacccatta tgggatacat tccaagtggt
    18301 tcaaggtaat tgtgattatg gaccaggatt cgaacagaaa aaagtgatcc aaactggtca
    18361 ggatacagta ttcatgactc acggtcattt atcgaatgtc cgatttggtt tgacacagtt
    18421 agcaatcgaa gctcgagctg caactgccga tatggtgttt tttggtcata cccatcaaat
    18481 aggctgtgag atggatcaga aaatcctgtt tttgaatcct ggtagcattt ctcagcctag
    18541 aggcccgatc caaatcccat catatgctct tatcgaaagt ttagatgacc aaatttctgt
    18601 tcaatactat aaccgtgccc atcaggcagt tgaagagttg gccttttctt tttcgagaga
    18661 gcgctgaata aaaaaactct aatttttaat aaaaataagc tttattttat gaattttgca
    18721 tccggtttcg gtaaaatagt agtgttgaat gaaatagaaa tggaggccat cagatgattg
    18781 gacctatagt aagagaaatg ctgctggaaa atcaggaaac gtttttgatt cctgcagaaa
    18841 atgtggcaaa tgttatgtat catcatccat tgtcccatgg tttacttgtg ttatcgaagg
    18901 tcggttattc caaaattcct gtattaggaa aagatgatca ctttgtagga ctagtaagtt
    18961 tatcagatgt ggtcaataaa atgattgact tgcaaacaat cagcatggac ccgttagaag
    19021 gactaacggt tgctgatgtg acagaaacag atgttccgac aattgatgaa aattgggaac
    19081 tagaagaagt actccatttg ttagtcgatt cgcctttttt accggttgtc acagaagatc
    19141 ggattttcaa aggaatcatt acaagaaaag agctgcttaa agccgtaaac ttcatggtgc
    19201 atgaattaga acgtcgtaat atattgtctc cgaaaatcga agaattaaaa gaacaaatcg
    19261 atatcgtgag ctaaagaacg agaaactaaa agataatcaa aaaataagta gaaaaaacag
    19321 cgtaccagtt ttgactttat cttgaaaaag aggctgggac aaaagcgttt aactccaaga
    19381 aataagaagg aattcacgaa a
//
LOCUS       DAGCWU010000042        19027 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.042, whole genome shotgun sequence.
ACCESSION   DAGCWU010000042 DAGCWU010000000
VERSION     DAGCWU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.042"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..524)
                     /locus_tag="MBZ71_001163"
     CDS             complement(<1..524)
                     /locus_tag="MBZ71_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBT4429130.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDP"
     gene            complement(526..1290)
                     /locus_tag="MBZ71_001164"
     CDS             complement(526..1290)
                     /locus_tag="MBZ71_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBT4429131.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1298..1975)
                     /locus_tag="MBZ71_001165"
     CDS             complement(1298..1975)
                     /locus_tag="MBZ71_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBT4429132.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2012..3397)
                     /locus_tag="MBZ71_001166"
     CDS             complement(2012..3397)
                     /locus_tag="MBZ71_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBT4429133.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3607..3691)
                     /gene="ffs"
                     /locus_tag="MBZ71_001167"
     ncRNA           complement(3607..3691)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="MBZ71_001167"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3714..4229)
                     /gene="tadA"
                     /locus_tag="MBZ71_001168"
     CDS             complement(3714..4229)
                     /gene="tadA"
                     /locus_tag="MBZ71_001168"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBT4429134.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4336..5193
                     /locus_tag="MBZ71_001169"
     CDS             4336..5193
                     /locus_tag="MBZ71_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4429135.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5274..5609)
                     /locus_tag="MBZ71_001170"
     CDS             complement(5274..5609)
                     /locus_tag="MBZ71_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429136.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5676..7046)
                     /locus_tag="MBZ71_001171"
     CDS             complement(5676..7046)
                     /locus_tag="MBZ71_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBT4429137.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7124..7660)
                     /locus_tag="MBZ71_001172"
     CDS             complement(7124..7660)
                     /locus_tag="MBZ71_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4429138.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            7969..9450
                     /locus_tag="MBZ71_001173"
     CDS             7969..9450
                     /locus_tag="MBZ71_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4429139.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTTKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHKLYMKKGYENKTKRM"
     gene            complement(9553..10260)
                     /locus_tag="MBZ71_001174"
     CDS             complement(9553..10260)
                     /locus_tag="MBZ71_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBT4429140.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(10339..10677)
                     /locus_tag="MBZ71_001175"
     CDS             complement(10339..10677)
                     /locus_tag="MBZ71_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBT4429141.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(10788..11750)
                     /locus_tag="MBZ71_001176"
     CDS             complement(10788..11750)
                     /locus_tag="MBZ71_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBT4429142.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGL
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(11765..13096)
                     /locus_tag="MBZ71_001177"
     CDS             complement(11765..13096)
                     /locus_tag="MBZ71_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4429143.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(13104..13784)
                     /locus_tag="MBZ71_001178"
     CDS             complement(13104..13784)
                     /locus_tag="MBZ71_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBT4429144.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(14003..14482)
                     /locus_tag="MBZ71_001179"
     CDS             complement(14003..14482)
                     /locus_tag="MBZ71_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBT4429145.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(14609..16288)
                     /locus_tag="MBZ71_001180"
     CDS             complement(14609..16288)
                     /locus_tag="MBZ71_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBT4429146.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            16836..18338
                     /locus_tag="MBZ71_001181"
     CDS             16836..18338
                     /locus_tag="MBZ71_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4429147.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            18351..19013
                     /locus_tag="MBZ71_001182"
     CDS             18351..19013
                     /locus_tag="MBZ71_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429148.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
BASE COUNT         5603 a         4008 c         3207 g         6209 t
ORIGIN      
        1 attggatcag taacgggatc gaataaccag tcatcgttgt tgaaaaacgt ttcatgaaaa
       61 aggataaaaa agcgatcaaa tcctatcatc atgaaaaaac cgaaaacaac tggcagtaac
      121 atgccgattt gaaacggacg aatcaaccgc caaagccgat cattcttttg caaatatttg
      181 ataaacaaaa tactcgggat caaagtgatg aaaaacacag cataatccac taaaaacaaa
      241 attttcactt cataaaagtg atgggctccg ctagcagaaa ctgggaaatc tggcaagctg
      301 aggatggttt tgaagggatt attgagataa ttcattaggt gatcaaaatt tttcagtaac
      361 gtctcttgat ctaaagaagt ataatccaaa atttgcaaat gtccgatatc aaatacgtat
      421 agtggtcgaa agttgatcgt cacaccaact gccagactga tcagtgtcag aaataggctg
      481 aacaatccag catattccat ccactgccat ctcgaatttt tcatattaga agctccattc
      541 atccagtgat tgtttcacat aggtagctgg gataggcaac ccaggtacat cttcttcttt
      601 tgtaaaacct gataaaacga gcaaagtatc tatattattg cgtatcccag cttggatatc
      661 tgtttcatag ttatctccaa ccatgattac ttcttctttt tgcagcccta atacttcaac
      721 ggctttatcc ataatgatgg cttctggttt tccgatgtag atcggcggcg tttgggttgc
      781 tgtctctaca aaagagatga ctgaccctgc tccaggaaga aggccgcgtt cagtaggtat
      841 attcttatct gggttcgttc caatgaaagt agcacctttt tgaatagcaa gtgttgcttt
      901 aactactttt tcataggtca gataattatc taagcctacc actacatagt caggagcctc
      961 ttcttcccat gtaaatccgg cagctaaaat caagtcaatc aatcctgctt ctccgataac
     1021 atagactttt ttcccttttc catctgcttt cataaaatcg attgttgcca aagtagctgt
     1081 gtagactgtc tctggagcta catgaatgtc aaactcatct gctagacgat tagctactgt
     1141 ttctggtgac ttagtcgtgt tattggtaac aaacaagaaa ggtaattttc ttttttgcaa
     1201 ttcttccaca aatcgtttac cggccggtat gggttgtgtc ccacgataaa tcgttccgtc
     1261 taaatcaatt aaatatcctt tatagtccat tcatccacta ctcttccctt tttctgatcg
     1321 taaatgctgt ttttgttttt tctacttgtt taggttgttc ttgttttttt actttttcag
     1381 aggtttttgc tgactctgtt tttcgatttt taataaccgg ttttttgcga ttagatgcga
     1441 ctggtccttt tttctcatcg atatacgcac gattatggtt ttttttcttt tttcggcgct
     1501 gagttgtctt ttctttttta ccgccgattc tctcgatgac aaaatacgca caaccaaaat
     1561 tgcaatattc gtaaagatag tcttccaagg aatcgattcg ctgatctgga tgcgcccgtc
     1621 gattatccgc ttggaaaaat ccttttaggc gtagttgttc ataaccccaa tctcctacga
     1681 tataatcata tcgggctagt acatcactgt aacgttctcc aagcttttct gcattaaatc
     1741 cttcacgata atcggacaca agccgataat gtctttcacc tatcaaaaaa ttactgtcat
     1801 ctaacagtgt cactagctcg cctttgactt tttctgcttt aggctcctca acgatttctt
     1861 ccaaagcagc tgtcagttct tcggtgactg ttgtttcctg ttcagctgat gattttttct
     1921 tagcaatttc tgttttatgc tcaacgttct gctgttcatt ttgttttgca gacatgcgtt
     1981 caccacctat cttttttatt ataccttgtt tttatttgat tgggtagtga gctttcaagt
     2041 aatctcctac cacacttcgg ataaattcag ggaacaaaaa ttcatttttt ccagcaatct
     2101 caatcgttgg aaaaaatgga acaaacatca aatgatctac ggtagtaaaa gtataatttt
     2161 tattcagatc gattggcttg ttcttccaaa agacttgatg gttaatagaa tcatatgtga
     2221 ttccattata gaccatctgc ccaaaaatct taccgcgaaa tcccattccc ttcataggat
     2281 agttacgtaa aaagttacga tttttttcta tttctaaaac taagcggatc agatcatttc
     2341 cacccaatgt gacatttaac aagtgcatcg gatgcggcaa cgcagtgtga agctgatcct
     2401 gattgacccg cccttttgga atttcagtta aaaacagacc actgtttaat attgctgctt
     2461 ccgtctttcc tcgttctttc accgcctcaa aggctacttg gataaacgaa tgctcattga
     2521 agatatctag actcagagaa aatggcaaat ctgctacttc tttttctttt aataattcat
     2581 gcccacgttc tagataacta tttatttctt gttcatcttc tgtaaaagct gtcattgttt
     2641 cggtagggat ggctttggct gaatgcttaa ggattttttt ctcttcatcg atggttaaat
     2701 ggacttcacc gacatactgc ccgtactttc cagcggctgc tatttggaca tcattgatta
     2761 cgtgaccttc tctgaataaa tgatgggtgt gcgatcctaa aataacatca attgctggta
     2821 tttcttttgc aatttggaaa tcatcctgaa tcccgagatg actcatcaaa actaaaacat
     2881 ccacttgtga acgtaaatct tcgactaatt ctggcaaaat atcataagga ttccgtatgt
     2941 cccagccatt cggattataa gtaagcggaa atggagcagt aaatgcaatt agtccgattt
     3001 tcgtctggtg tttcgttgta atgattttat atggtttcgt ccatgccggt ggctggagcg
     3061 tatttttatc gaataagtta tccaggatga tatcaaactc agcatgatcg tataaatgat
     3121 tcaactcttc tttggaattt cctacgcctt cattgtttcc tatcgtcgct gcatcataac
     3181 caacttcatt catcagcaag atattttcct gtccattcgt tgcttctgac aatggatgcc
     3241 aacgatccac aaaatcacct agatctactg caatcgttgt cgttttttct ttcttttcgt
     3301 tttctcgttt acgttgatcc aaaaaccttc tgatccttgg ccaattttct aaatgagaat
     3361 gcagatcatt cgtatgtaaa atcactattt tttccatgta ccctctcgcc tctctttatc
     3421 tttatcatac cataggcagc tttttcttcc attgaaacaa gctctatttc agagtttttc
     3481 ctgccttgaa gcaaattgta taagtacaat attcgataaa tcagtctggc taaaaaatca
     3541 ggaacattca tttttacagt actataaaaa taaaaaaaac agacccgaaa gtctgttgtc
     3601 gtttcattat aatcagacac atggcggaac ctgcttagtg ctgcttcctt ccagacctga
     3661 cacgcttcac aagcccacca ttgtcctagc ctttcggcaa agaatagtat accttatttt
     3721 tcattcgttg accagtcttt ttttaatttc ttttttcgct ggcggagctt tcggaaaaag
     3781 tcagtcaaaa tcatcccaca ttcgtcttct aaaatgccac cttctacata tgcttggtgg
     3841 ttaaaccgct cgtcctccaa aagattcatc aaggtaccgg ctgttccgcc ttttggatca
     3901 tacgcgccga aataaacttc tgctacacga gacaaaatca tcgcgccgct gcacatagga
     3961 caaggctcta aggtaacaaa aagtgcagca tcttccaagc gccagcttcc cactttttca
     4021 caggcttcct tgattgcaag catttctgca tgtgtagtag catcttgcgt ggtttctcta
     4081 aggttatatc ctcttccaat cacttttcca tccaatacaa ccactgctcc aatcggaact
     4141 tcatgaagca tttctgcttt ctttgcttct gccaatgcaa ggcgcatcca tttttctttt
     4201 tctactgcat caagatttgt atttaccaat tacccttccc tgcctttgtt ttttcttgtt
     4261 ttactttagc atatgataaa aaggctgtaa ctaacttttt tttctatggt aaaataaata
     4321 caagaggtga acaagatgaa aatggataaa ttacttgctc tttacccaca agcacagaaa
     4381 aaaaactctc cagcatcaga agcggatatc ctttcttttg caattgatga tgcttttatc
     4441 tggatcaaac aagattcatt atctcaacaa gaaaccagcc ttttgaaagc gttattccct
     4501 gtaatcaacg atcaaaaaaa acacccatgg tatcactatc tttttaaaga tgcttcttgt
     4561 gttcaagaaa aaagctttcg tttgatccag cttcacgtcg aatcaaaagg agaattctta
     4621 aaaaaagaat ggcaagatac tattttggaa ttatttcgct attctgctga tttctttttt
     4681 tatacagaca cagatgcgct cttagtcgaa caaataaacc gtgattatct tgatgctact
     4741 gaaatcaacg gtatttttct atctttagat gctgattttg atacgacaac gtctgtattc
     4801 gttggttctt tccattcacc tggcaccgac ctcgtccctt tgtttgctga agaaagaaaa
     4861 atttttttaa cagagcaaaa caatctttat actcacagcc gtactttttc tatgcaagat
     4921 gtggctttac attactatac aaaagatact gtaaaagata gtaagttgat caaaagttac
     4981 atagaactga tcaaacaaaa tgaaatggaa gtgatcattc aagaattatg gaatgaatta
     5041 ggcaatgtca gctctgctgc aaaaacactg tttcttcatc gtaatactct aaaatatcga
     5101 atcgaaaaat ttcaagaaca gtctggtttc aatctaaaaa aagccaacga tttactgttt
     5161 tgctatcttt tattgcttca tgaacataat taaagataga ttaaaggggt tgtgacaata
     5221 attttgtcac aaccccttat ttcgaatgaa tagtgttcaa aagctagttt ctcttacatc
     5281 attttttcaa caaaatgcat tagccggtct tttacatctt cggttgaatc tttgattgaa
     5341 tcaccataaa cagagatttt cttttttcct gatttgattt ttgctaacat tcccatcaaa
     5401 gaatccaagt cactatctga aagaccatct acgatttcta gtaatttttc atttccatca
     5461 aatttgtcat agacgaattt ttttactttc gtccgagtag tcatatgatg gatcttttcc
     5521 attacttttc ccgatacaac gactgctgtc gctacactgg cagcggctgc tacgcttaat
     5581 ccgattgtta cttttgttga tgttttcata agtatcagct cctttttatc tatctccatc
     5641 atagcataga caaaaaaata aacgaactaa tccactcagt gaaatctcaa tcctttaggg
     5701 aaaaagttct ttactgttcc ttttacttgt ttcccaaatc cgacaggtat cttatctaat
     5761 actagcaaaa cccatccttg attcccatca cgttgaaatg tttctcctgc tgtgtaagat
     5821 tgccattcct tttgagtgat cggcaagcaa ggtatattct ctatcttttt tgtagcaagc
     5881 gctaatgcat agctaggttc aaatcgattc tttttgaaat ctcctaaatg caaacctgta
     5941 cgtacgactt ttaaaccatc gagagaaggg gctaactcag gaacttccca aagatggtcg
     6001 ttgaacacta aaagtcttcc agtagcttca taatgaaaat catttgaaaa ctccgtccag
     6061 agtttttcct gctcttttgt catttgcact ttactttttt ttcgtgtctt cttttcttta
     6121 tgcatctgat tttgtccatg aaaagtcagc tttgcaacaa aatgtccttc accctgatct
     6181 ttatgaggcc atatacgtat tgttttttcc aaaccagcta cgcttcccca ttcagaacgt
     6241 cctgaactaa cggattgagt caaaggaatc tcctcaatcg ttactggata attttctaca
     6301 agccagctaa tgatctcttc attttcttct ggtgcaaacg tacaagtcga ataaatcagt
     6361 tgtcctttat tttttagcat ttttattgct gaagacaaaa tctcttgctg acgtttttga
     6421 cagtatagcg gagattcttc tgtccactct tttattgcat ttggatcttt tcggaacatt
     6481 ccttctcctg aacaaggagc atcaaccacg atccgatcaa aaaagcccga aaaatgaggg
     6541 acaagttctg cgggtgcatg atttgtcact attgcgttcg aaactcccca tcgttctata
     6601 ttttctgata aaatcttcgc acgtttcgga aagatctcat tcgtcactaa taaccctttc
     6661 cccttcattt gagcagctaa ttgggttgat ttaccaccgg gagccgcaca caaatccaaa
     6721 actttttcac cgggttttgc tgcagcagct gttccaacga tcattgcact tggttcttgg
     6781 ctatattcat acccagcttg atggaggaaa gactttccat tgacagtgcc aagaaatccc
     6841 tcattgctgt agggagcggg ttgtaattct tcgctatggt aagtttggac catgtccaat
     6901 cctgccggtt ttagtggatt ccaccgaaat ccttttttca cgcttccttg ttcaagcgca
     6961 gaaaaaaaat cggatgcttc ttcgcctaaa agaagacgat attttttgat aaattgctgt
     7021 ggtaacgttg tcgcttcctc cttcattcca atgtttcgtt agacatttta caatcttttt
     7081 tacgaaaaat aaaggtggct gatctacttt ttcatgttgc tgcctaaggt gactgttcct
     7141 ttaagatatt ttccaagtat tcatccgtca cctcgttttt atcccaaaca gcgttcatca
     7201 tttcaagaaa atcttgagaa gaatcatctg agcgcaaact tcccaatgca ttaagccata
     7261 ccaattcaag cgacttttct tctttgatct tcgtgtgacc tttgacatat gagaaaaatg
     7321 gccgcttaaa tagcgaatcc atgtctaaat cagattttgc aatgatttca cgaatacttt
     7381 catggataga aaaaaaatct ttcaataagt agacaatgct taaaacatca tcggaactgc
     7441 attcagctaa gtagttttca aaataaacag tgatttcttc taatatcttc tcttttaacc
     7501 cgctgattga tttgaaatgt gtataaatcg ggtatgtaga acaaccgcag aactctgcta
     7561 actctcttgc agtaattgac ttcattccat attcttccac gtataccata cttttgaaca
     7621 gcaaatattc tttactataa tttgttactc tccccatcat tcttacctcc gtctcttttc
     7681 atctacccat aaataaataa cataaataat tctaaaaata ttattgataa ttatcaaaac
     7741 atatttagtt atatccatta aaaatcaatt tttccgcctt aaaaacaaga atatcttgac
     7801 aaaataccct aggaaataaa caatagaata aacaaggtaa caaaaaaatt aaataacgtt
     7861 atataaaaaa gaaggcgaca ctctgtgaga ataacacaga ctgaaatgac tttccactca
     7921 cagtttttgt ctaactatat acaaaaacag aataaaaagg aggtatttat gctaattgaa
     7981 gcaagaaaaa aagagttact tgttctacaa atggtcattc aaaaaaataa ttgttcttta
     8041 caagaattat ccaatgactt acatattcca aaacggacaa ccaaagaact gatccgaaaa
     8101 ataaatgtat cactcaagca atttttattg attgatgaat ttatttattc taatcataaa
     8161 ggagagatcc aaaccaaaga aaccgatcaa actaaaaagt tagctgtttt ttctaagtta
     8221 aaattatgct acctaaaaga atccaatcgt tttaactatt tgatgttact aatcaattat
     8281 ccacaaactg ctgtaccaaa aaaatattta cttgaacaac tttatatatc gccctcctat
     8341 ctcgaaaaac tgacaaaaca gctcaatcat tccttaaaaa aattccattt acggatccta
     8401 tctgtaaaaa attgctgtac tcttgaagga aatgagttat ctatccgtct ctatctttac
     8461 ttttttcttt cagatacatt tggaggtaac gattggccat tttcctcact tgaattaaat
     8521 gcactaaaaa tgaacagtac agatgagcaa atagaaaaaa tttcaaaaca agaaaaaaat
     8581 atctacttgc tgttaacttt attcatatta cgaaacaaac aacaggcata ttttccaaaa
     8641 acttcttctg aaatcactga attgatggac ttattgcagc atacacatga ttattcagaa
     8701 tactttactt ctttttcagt gagtcgccct tgtgaagaga cagaaacaga gagagcctac
     8761 tttaattatt ttttctattg tttttttcca aatcaaatct cttttcgaca aaaacaggag
     8821 attggttttt tattttccac aagcaaaaac cttatttgtc gacaagtcgg tcaattgatg
     8881 acttacttaa ctcaacagtt tcctctcaac catttagaaa ataaaatacc aatttatttt
     8941 tattttcttg tgttggcagt tgtttttttc gaactgttgg gtgaatccag cagtagtttt
     9001 tctacacttt gctttccaga agccacgtcc aatcattcta gttcttttat acaggaaaag
     9061 aaattaagag aaaaaatcga tacaattttt tcaaaaaatg gggaaagaaa ccaaatcagt
     9121 caatacctta gtcaattgat ccaacactta ctacaaacag aaacgaaaga gcagttactc
     9181 atccatttgc agataaccaa acagtttaca ggaaattatc atcttcaaag ccatttgcag
     9241 cagttatttc gcaaagatgc tgttaaaatt accgcatatt cagaacatgc tgatttgatc
     9301 attacagatc agcatgagaa aatttcagat gaaacggaga ctttctatct ggatacaacg
     9361 cacaatacag tgttatttgg caagttaata aaagcggttc ataaactgta tatgaaaaaa
     9421 ggttatgaga acaaaacaaa gaggatgtga caaaattctt gtcacatcct cttattccaa
     9481 ataaatggta ttcaatcagc taactctcgg tattagctca gatcaaagcg ctgatttcac
     9541 aacttcttta ttttaatctc ttcggttgcc gccgaacaat aagatcaagc gcaacagctg
     9601 caggaatgtc gaaagcgctg ctgcaacata agtcagtgct gctgcaaata atacgtgtct
     9661 tgccatcggt acttcttctt ccgtcagtag tccgccttca ctcaaaattt ggattgctcg
     9721 ccgagacgcg ttaaattcga ctggtaatgt caccaattgg aaaagtaatg ctaaagaaaa
     9781 agcgaaaatc ccgatattga ttaacgtaga gttccagcta agcaatacac ctaccataat
     9841 caaggggaac gaaatcatcg aaccaaagtt agctgctgga acaagtgctg cacgaatctt
     9901 caatgggaca taacccgaag catcttgtac cgcatgtccg cattcatgag ctgctacacc
     9961 tatcgcagca actgaagtag actgtgcagt tgcttgagac aaactcaaca tcttattacc
    10021 tgaattgtag ttatctgtga gatctccggc aatttgttga acaccgacat tatttatctg
    10081 ttgactttgt aatatatatt gtgctgcttg tgtaccagtc acattgtttt tgcttctcac
    10141 ttgatcgtat tttcggaaag tactattgac gtacgctgac gcaattccgg aaataactaa
    10201 tccaagaatg accaaaagaa atgtcggatc taaaaaacca tacattccat aaccatacat
    10261 tttcgtttca ctccttttcg tttactttat tttatcatta gtagtaggaa aagaatgtga
    10321 aaagaccttg aatattcttt aattcttttt cacaaattcc gatttcagtt tcatcggtcc
    10381 aaatccttct accttgcaat cgatattatg atcgccttct actaaacgga tgttcttgac
    10441 ttttgttcct tgtttaatgg cacctgacgc accttttact ttcaagtctt taatcactgt
    10501 cacgctatcg ccctctaaca gacggtttcc atttgcatct aataccacta atccgtcttc
    10561 tatgacagtt tcttgttctt ctttgttcca ttcgttcccg cattctgggc aaatcaacaa
    10621 gcctcgatct tcatatgtgt actctgagcc acattctggg caatttggta atgtcatctt
    10681 ctttctcctt taaaatcttt agtgagcact attgtaacgg aaaaagaaga tcttgaaaag
    10741 atacatttgt gatttttact acaaaaggca cgttttcgaa cgaagtatca ttctgttttt
    10801 ctgctcttat acgattggca tatatcttgg atctctttat agatttcctc tacttctgct
    10861 aaatcttttg cattcgcacc acttgctaat ggatggcctc ctccatgatg gcgtttagcc
    10921 aattcattga tgatcggccc cttagaacgt aaacgtacgc ggtaataccc tgaaggctgc
    10981 tgaacaaaaa tcccccaagc cagcacttcg tcaatcactc caggaagtga aacgatcgaa
    11041 gctgtctctg aatcatcgat tccatattta tcaagaattt cttgcggaag gattattctt
    11101 cctgcaccat tttcatccac ctggatattt tgataaagat aaccggaaag tttagcgact
    11161 ttcatcggca tttgttcaat ctctctgttc aaagcacttg catcaaatgg ataatttctc
    11221 aatatcgaag caatctctaa tgtacgggac gtcgtggagg gatacaaaaa gagccctgta
    11281 tctccgacga ttcctgcata aagtaaccgt gcagccttaa cgttcatctt taaagattca
    11341 ggaaacatca aagcaaattc tgcaatcatc tcactacagc tacttgcttt tgtatttacc
    11401 cacacaagat ctccataagg gtcatcattg ggatgatggt cgattttgat cagtttttct
    11461 gccaactgat atctttgatc actgattctg ggtgaattag ctgtatcagt cacgatgact
    11521 agcgctcctt ggtagaagac atcttcaact tcttgcattt ctgctaaaaa gcgaagccct
    11581 tcaatcgttt cacctacttg acgtatttct ttttctggga aactttcccg taatagttcg
    11641 gctaatccta cttgtgatcc taatgcatca ggatcagggc gcaaatgcct gtggataatg
    11701 attttatcgt atttttttat tgtatctact atttcttgct gtactgtcat ttcactcctg
    11761 ctttctaaga acgttccatt acttgacaaa caacgattgc tttggaaacg atggtgtttt
    11821 caataaacac ttcgatatcc agtttagcgg aacgtctgcc gatttccaat acacgtggac
    11881 gaatgtctaa ttcgctttcc aattggatca gccgcaaata atgtaggttc acttgttcaa
    11941 tcaagatatt tcgtttttgg ttcatcacca ttgttttttg tgcgacatca gaaatgattt
    12001 cgctaagtac gccaaaagaa atggttccaa cgccattgac catttgtggt gctactacga
    12061 atttgaactt agggttttcc aaacgatttc cttcacggtc agtatcaatc gtatggatat
    12121 ttccagatat ctgatccgaa atcgtatctg caatctgcgg ttgtcgctgg acaagctgca
    12181 tcgctttcat tacatcttgc cgggtaatca agccttccaa tgtcaagtca tcggaaacga
    12241 ccggcatgac ttctagccca tcccagatca tttgatggct aacagaggca atactcattg
    12301 ttttctttac actgattggt tctttcgtca tcacccgatc gatgatctgg tttggcgatt
    12361 tccctaaaac atcttttgcc gtaacaatcc ccatcaaacg atgatggcgg ttgacgactg
    12421 gaaaacgcga atgctgcgtt tcttctgaca aacgctgata atcagaaatc gtatctgtct
    12481 gctgcaaata atgagtcttt tcagctggca tatagatatc actgacaagc atgatatctt
    12541 ttttgatcaa ttggtcgctt aacgctcggt tgatcatggt tgcgactgta aatgtatcat
    12601 aagaggtgcg taagatcggc atttcaagat catcagctag ctttgcaatc tcagcagtcg
    12661 tattgaatcc tcctgtgatc aatactgcgg cgccatcttc caatgctaat ttttggactt
    12721 ccgcccggtt gccgacgatc attagtgatc ctggcgtaat atagcgttcc atggcatgtt
    12781 tagtcatcgc gccgatgacg aatttattca agaccttgtc taatcctgcc gccccgccta
    12841 aaacgtcccc ttcaatgatc ctgacgacct cgccaaatgt caaacgttca atatgttttt
    12901 ttaatttgcg ttcgatacgg atggtcccta ctcgttgaat tgttgaaact agtccgacat
    12961 tttctgcatc tttgattgca cggtacgctg ttccttcact gacattcaat tcttttgcga
    13021 tgcttcttac agaaattcga ttgccaacag gtaaactttc aatatattct aaaatcaaat
    13081 catgttttgt cgccattttc tgcctacact tccagcgttt ccgagacgct cattattttt
    13141 ccttcactat cttccagtaa ctctacaaaa gattcgggat cttgttcaat gacagggaaa
    13201 gtattgtaat ggattggaac tactttcttg gcacgaattg tttgcgcagc ctttgctgca
    13261 tcttctggcc ccatcgtata gttatcgcca atcggcaaga aagcgacatc aatatcgaat
    13321 tcttctgcaa gcaatcctag atcactgaaa taagcagtat cccctgcatg ataaatcgtt
    13381 tttccttctg cttgaataat aattccgcta ggctcgccca tatactgaac gataccgtct
    13441 acttcatagc ttgaactatg ctgtgcatgt acgaatttta ccacgccgaa tggaaacgca
    13501 tgttttccac cgatattcat accgaatgaa tctactcctc tgctgttcgc ataatcagca
    13561 atctcaacaa tactgataat tgtggcttga ttttcttttg caataggaat catatctcct
    13621 atgtggtcac tatgaccatg agtaatcagc aaccaatctg tctttacttc ttctgcagtt
    13681 aagtctgtca atggatttcc tgtaataaac gggtcaatca tcacgcgttg gccattttcc
    13741 atttcaattg ttaaacagga atgtccatga tacgtaattt tcatatttta cacctcgcgt
    13801 tttctctgta tcttttttat taaaaacgat ttcatcactt gcacatcaat gaaaaaatat
    13861 agctgctaaa tatattttca ttggattgat agtacagtat gtacaaagca attgtaacag
    13921 ataatacagg atgcaccaag taccaagcag atataatctc aagtttgagg tctctgctct
    13981 gcttagcttc tcttctcgtc ccttaactaa ttaaatcagc gtaaatagga tggacgatct
    14041 tttctaaatc ggctactttt atccgaaggc ttctcccgat ttcaccgctt gacacaatta
    14101 cttcttcttt ttcttttaaa gaactgtctg ctaaaaaaca ataatcagga tgtgcgagtt
    14161 ttattccaat aggggtgttt gctccgtggg gatatcctgt atgcattaat acaaaatcca
    14221 ttccaggaaa accaacttta cgatctttcg tcacttgtct cgtttttttg taatctaagt
    14281 gatcgttcaa aggaatcaat gcgatgatca ccccagtttt gtttcctttc actacgagag
    14341 ttttacatat caaagctgga tcaatccctc ttttttgtag ttcttctgtg aaagattctt
    14401 ctaccacatg actaaaatca agtggttgat agctgatctt ttgttcttct aaataacgtt
    14461 cgacttttgt ttgactcatc attgattcac tccctttatt ctatcgttta ttataaagcg
    14521 agggatgctg tattcaaaaa ttatttgttt ttcgcaaaaa aagagcaaga cgagcactct
    14581 atagagaggt tcatcttact ctttttgttt attttttatt tcgacgttga gtagcttcca
    14641 taatgactgg caagatcact gggcggcgtt ttgtttgttc aaataagtaa cggcctaatt
    14701 gatctcggat gtcttgcttt aatttgctcc actcaaaatc attgctttct aaatgtttct
    14761 caacgattgt ttcaatcata ttgctgcttt ctttcatcag atcacggcta gcttttacgt
    14821 aaacgaatcc ccgtgaagta atttgaggtt ttgaaatgat tttcttttct cgccgactga
    14881 tagtgacaac cgctacgaaa atcccgtctt ctgacaagat ttttcgatca cgtaaaacaa
    14941 tgtttccgat atcacctatg cctaatccat caatcattac attttctgca cttgtagttc
    15001 ctgcaacact catacgcttg ttcttatatt ccagcacgtc tccgcgtcca gtaatataga
    15061 tatttttgta tggcatccct actgcatgag ccaaatctgc atgagcagct aattcacgat
    15121 actctccttg aactggcaca aaatatgttg gtttcatcaa attgatcatt aattgcaagt
    15181 cattaggatt ggcatggcca gaaacgcgca tattctcaga gatcaacttc actgttccac
    15241 cagctcgata aatgatatct tccgttttcg ccacgatggt ttccatcgcg atgcttggcg
    15301 tagtagtgat ataaaccaaa tctccttctt caatcttgac attacggtgt gtaccgtttg
    15361 ccattttttg cagagacttg atgggttcac ccattcggcc agtttctaaa atgatcagtt
    15421 cttctggatc atagtttttc atgtctttca acgtaacaag cagatcttca cttggtaatt
    15481 gaagtttttc taaacgcatc gctgtgcgaa taattcgttc gaaatcttga ccagtcaaga
    15541 caacttttcg ttgtgctttt tctgctgcat ccaatacttg ttgcactcgt tgcaaattac
    15601 ttgctacaca agcgacgatg atacgccctt cccaatattt gatcgtatca aatacttcat
    15661 ctgctatttg ggcttcagaa gctacaggct gaggattttc tgcatttgcc gattcactta
    15721 gtaaggctaa gactccttct ttgccgattt ccgccaatcg tgcataatct gtttgataca
    15781 ttgggatagc cgtttggtca aatttaaaat ctcccgtata aacgatacta ccttcttttg
    15841 ttttcagatt gatccccact gaatctggta tcgtatgagt ggtacggaaa aagctcacta
    15901 ctgtttcacc aaagtctatt tctgtatgct catctaccac atggaaatcc ttaaatcctt
    15961 tggctgcttc attacgactg acattcaatt ttgctaactc gatcgtcaac tctgtcccga
    16021 atactggtac agaaattttt gacaacaagt aaggaagtgc cccgatcgca tctgcatgtc
    16081 catgtgttaa aaagacacca gctacacgat caatgttttc ttctaaatac gtaaaatcag
    16141 ggattacgac gtcgatcccc aacaactcat tttcaggata ttttagccca caatctaaaa
    16201 taaaaatatc ctcgcccact tctgcgacat acatgttttt tccattttca cgaacgccgc
    16261 ctaatgggat aattttaatg gtactcacaa acttcacctc ttcttatgac atccaagttt
    16321 aaagatgtcg attttcttct ttatctaaat atatgagcca ttccaatgaa gcatatgcaa
    16381 aatcactatt tctcaactta tggagaatca gcaggaaaat taccagcaca tccgcgttct
    16441 gacagttgga tgggcatttt attttggata tacttcatta acaaaatgga tcttttgcga
    16501 aacgctatag ccagaatact tatataagta ttcagctgtt ccccattatt atacaacaaa
    16561 taaactaaat cgctagtatc agcaattgtt agaacaacta tgcacgtaaa aatccctctg
    16621 tagcatctag gatttctttt gctattcctc tttcatttct tcttcgtcgg tttgaaacat
    16681 acttttttca ttttaccaca ttttttgcaa taactatagt gactttttat atatacagaa
    16741 cctcatgttg atggtgtgca aacagcataa gatttaagct agctttaaga taggttgtct
    16801 attatcaaat gcatctacta ggagggatgc aaaatatgaa ttttatgaaa cgtgcatgga
    16861 aaagtacaaa agtaaaatgg ggacgttcat tgttattgtt tgccgtgttc acagccattc
    16921 ttatttttgt gctagctggc ctcacgattc gaagcgctgc agaagaagca gcgaatcaag
    16981 cacaaaaaga agttggagca acagtgactt taagtgccaa tcgagaagct tcatttaaaa
    17041 aacaggaaca aacttcttca agcggttcta ccactgaaga acgtccagat ccaggcagtt
    17101 tttctcttac accagtttcc ttaacggacg cggaaaaaat agctgaatta gataatgtaa
    17161 agagttattc ctttgaaagt tccgcctcag ctatggcaaa agatggaata actgccatca
    17221 ctagttctga ttcaagtgaa gacagttcag aaactactga tgcaggcggt gaacctgctg
    17281 gaggaatggg cgggaatccg caaatgactc aagcagactt tcaaatcagt ggtgtgtcag
    17341 caactgcaca aaatagtaat ttttctgatg gaacagcaaa aatcacggat ggtgaaggaa
    17401 tagatgacag cgacaaaggc acaaacaatg tagtcattga ttctactcta gcagaaacaa
    17461 atgacctggc agtcggagat actttcacaa tcacgagtac tgaagatgaa gataccactt
    17521 atgaaatgac gatcaaagga atttatgaat ccagcgaaac aagcggcagt atgggtatga
    17581 aattcaactt catgaatccg tcgaatacac tttacacttc ttacactttt gcaaatgaac
    17641 taaatgggac atctgacgat aacacgatag attctgctat ttacactcta tctgatccag
    17701 ataaaatgga tgagtttctc aaagaagctg agaagctgat cgacacagat acatttagct
    17761 tacaatcaaa tgattccatg tatcagtcaa tgttggaacc tttaaacaac gtggcaagtt
    17821 tttctaaaaa tgttgtttta ctagtggctg tagccggtat tatcatactt actctgatca
    17881 ttatgattac cattcgggag cgtcggcatg agttaggcgt cttgctatct ttaggagaat
    17941 ccagaagtaa aatcattctc caattgttta ccgaagtggc tatttgtatg attcttgcat
    18001 taggagttgc ttcgcttagc ggaaacgtcg tcgcgaatgc cgtgggacag cagctacttg
    18061 accaacagac agaaaccacg acacaaaacc agcaagctcc tggcggtgga caaatgccag
    18121 gtaacagaca agaacaaggc ggtcaacgcg atggtcaaca aggaaccacg aataatccat
    18181 ttgctgtttc tgatcaagtc agcgaattgg aaatcaatgt caaacctgca caattgggac
    18241 tattagccgg agtagcattt ggaatcagtc tgttttctgt ctttttagct tctataggga
    18301 tattaagatt aaacccaaga aaaatcctat taaactaaag gaggaatttg atgttacgga
    18361 cagaacaatt gggattttgg tatcaagatc cgcaagatgc gctttataaa gatgtagaac
    18421 tgaatttcga agctggaaaa atgtacgcta ttttaggagc aagtggttct ggaaaaacga
    18481 cttttctttc gcttatttct ggactagatg caccaaaaga aggaaaggta tactatcaag
    18541 atcgttcact gaacaaaatc gggttaagaa gttatcgtaa aaatgatgtc tcgattattt
    18601 tccaagctta caacttgctt ccttatttat cggctctaga caacgtcata gcagcaatgg
    18661 aaatcactaa gaccaagcaa gagaattatc gacaagttgc tttagatcga ctggctcgtg
    18721 taggaattga tgaaatatta gcaaaaaaac aggtgaccaa actttcggga ggtcagcagc
    18781 agcgagtcgc tatcgttcgt gctatctgct gtgaacatca actaatcgta gcggatgagc
    18841 ctactggaaa tctagatgaa acaacttcac aagatatcgt caaacttttc caagaaatcg
    18901 cccatgaaca aaatcgctgt attatcatcg tgactcatga gattgaagta gcaaaaagct
    18961 gtgatgaagt ctttgaacta aaaaataaaa aatttcaaaa aatcgaagtt taaaaaacag
    19021 gataatg
//
LOCUS       DAGCWU010000043        18618 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.043, whole genome shotgun sequence.
ACCESSION   DAGCWU010000043 DAGCWU010000000
VERSION     DAGCWU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.043"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            103..855
                     /locus_tag="MBZ71_001183"
     CDS             103..855
                     /locus_tag="MBZ71_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4429149.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            852..1766
                     /gene="pfkB"
                     /locus_tag="MBZ71_001184"
     CDS             852..1766
                     /gene="pfkB"
                     /locus_tag="MBZ71_001184"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBT4429150.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1784..3715
                     /locus_tag="MBZ71_001185"
     CDS             1784..3715
                     /locus_tag="MBZ71_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4429151.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3826..5385)
                     /locus_tag="MBZ71_001186"
     CDS             complement(3826..5385)
                     /locus_tag="MBZ71_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBT4429152.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5540..7774
                     /gene="pcrA"
                     /locus_tag="MBZ71_001187"
     CDS             5540..7774
                     /gene="pcrA"
                     /locus_tag="MBZ71_001187"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBT4429153.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7791..9827
                     /gene="ligA"
                     /locus_tag="MBZ71_001188"
     CDS             7791..9827
                     /gene="ligA"
                     /locus_tag="MBZ71_001188"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBT4429154.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9933..10238
                     /gene="gatC"
                     /locus_tag="MBZ71_001189"
     CDS             9933..10238
                     /gene="gatC"
                     /locus_tag="MBZ71_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBT4429155.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10238..11710
                     /gene="gatA"
                     /locus_tag="MBZ71_001190"
     CDS             10238..11710
                     /gene="gatA"
                     /locus_tag="MBZ71_001190"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBT4429156.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11707..13137
                     /gene="gatB"
                     /locus_tag="MBZ71_001191"
     CDS             11707..13137
                     /gene="gatB"
                     /locus_tag="MBZ71_001191"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBT4429157.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13147..14202
                     /locus_tag="MBZ71_001192"
     CDS             13147..14202
                     /locus_tag="MBZ71_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBT4429158.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14313..15686
                     /gene="rlmD"
                     /locus_tag="MBZ71_001193"
     CDS             14313..15686
                     /gene="rlmD"
                     /locus_tag="MBZ71_001193"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBT4429159.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15689..15817
                     /locus_tag="MBZ71_001194"
     CDS             15689..15817
                     /locus_tag="MBZ71_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBT4429160.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            16241..16429
                     /locus_tag="MBZ71_001195"
     CDS             16241..16429
                     /locus_tag="MBZ71_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBT4429161.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            16507..17520
                     /locus_tag="MBZ71_001196"
     CDS             16507..17520
                     /locus_tag="MBZ71_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4429162.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(17587..>18618)
                     /locus_tag="MBZ71_001197"
     CDS             complement(17587..>18618)
                     /locus_tag="MBZ71_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4429163.1"
                     /translation="VGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAAL
                     DYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYNL
                     SDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLER
                     MTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKYM
                     SYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIVV
                     ASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVRE
                     SFIPRTKRY"
BASE COUNT         6242 a         3429 c         4022 g         4925 t
ORIGIN      
        1 tttttttttg actgatttat aatttttatg aacgtttttg actgatttat attgcgagta
       61 gaaaaaaaat atgctattat ttgtattgag gtgatcgtga aaatgcttac agaggaacgt
      121 agacaaaaaa tcctagactt gcttgaacag cagaaaatcg tgaaatcaca agatcttgta
      181 aaccttttta atgcttctga atcaacgata agacgtgatt tacaagagct ggagaatagt
      241 gggtttctag aaagagttca tggcggagct aagaaagagc aattattgaa ttttgagcag
      301 aatatgagtg aaaaatcact caaaaacgtt cacgaaaaac agcaaatcgc caaatcggca
      361 gctgaaacca ttaatgacga agatgtcatt tatttagacg ctggctctac tacgttagaa
      421 atgattcctt ttctgcaagg aaaacagatc acagtagtga caaattccgt tcaacatgcg
      481 gcaaatttag ttgatttgga gcttgctacc attattttag gtggaacgat aaagctggca
      541 actaatgctg ttttaggatc taatgcgata gaacaactaa aaaattttca ttttaataaa
      601 gcatttatgg gaatgaacgg tgcccatctt gaacaaggat tcacgactcc ggacccagaa
      661 gaggcagctg ttaaacgttt agccatgaaa aattccgaag agacatttgt attgattgat
      721 cataccaaat tcaaccagtt gtccttcacc tctgttgcac ctttggaagc agccacgatc
      781 ataaccgagc gttgtccatt agaattcgtt aatgaattcc gtgaaaagac aacaattaag
      841 gaggcaatac aatgatttac acagtaacac ttaatccatc aattgattat atcgtacgcg
      901 tagatgggtt aaaactaggc gatttgaacc gaatgaagga agacttcaag ttacctggag
      961 gaaaagggat caatgtttcc cgtattttga aacggatcga ttctcaatct acagcactag
     1021 ggtttttagg agggttcact ggcgaattta tttcgaagtg gcttgaaaaa gaacaaatcc
     1081 agtctttgtt cacaaccgta aaagaagata caagaatcaa tatcaaacta aaatcagata
     1141 ctgaaactga aattaacggt ttgggcccat ctatttcaga tgaagaaatc gatgagttga
     1201 agcaagcatt aggaacagta ggacaaggag atatcgtcgt attgtcagga agcgcgccgg
     1261 ctagcctgcg aaaaggattt tatcaagaac tgattgatat cattcgagaa aaaggtgcag
     1321 atttcgtgat tgatacaaca ggtgatgacc taaaaaatgc acttgttaag aaaccattgt
     1381 tgatcaaacc aaacaatcac gaattagctg agctttacaa tacaaccttc caatctgtag
     1441 aagaaatcat tccgtatggt aaaaaattgc tagctgaagg ggcacagaat gtgctgatct
     1501 caatggctgg agacggtgct ttgctcttca cggaacaagg aacttataaa acgaatgtat
     1561 tagttcgccc attgaaaaat tcagtaggtg caggggattc aatgattgcc ggctttattg
     1621 gggcttacag ccgtacgaaa gatccaatcg aagcctttaa atggggagtt gcttgcggaa
     1681 gcgcaacagc tttttctgat gacttggcag ctcgcgaatt gattgatgag ttgctgccag
     1741 aagttgaaat cacaaaaatt gattagtttc aaggaggata atcatgaata tcaaggattt
     1801 aatggtcaaa gaagcgatga tcatggattt gcaagcgact gataaaaaag gcgcaatcga
     1861 tgaaatggtc caaaaaatgt ttgatgccgg ccggatttcc gatatcgaaa catataaaga
     1921 aggaattcta gcccgcgaag cacaaacttc tacaggtctt ggtgatggaa tcgccatgcc
     1981 tcacgcgaaa aacagtgcag ttaaagaagc aacagtttta ttcgcaaaaa gccaaaaagg
     2041 agtagattat gaagccttag acggacagcc aacttatctg tttttcatga tcgcagctcc
     2101 agaaggcgca aacgacacac atttgcaagc attagcagca ctatctcgat tgctgattga
     2161 tgcagatttc gtaggaaaat taaaagaagc aaatacacca gaagaagtcc aagacttgtt
     2221 ccaaacagct gaagagcaaa aagaagcaga agaaaaagca gaacaagaaa cacaagctgc
     2281 agaagcttct gctcatccag aaagaaaatt cgttgttgcc gttacagctt gtccaacagg
     2341 gatcgctcac acttacatgg cagaagatgc tctgaagaaa aaagcgaaag aaatgggtgt
     2401 ggagatcaaa gtcgaaacga acggttcaga aggaatcaaa aaccgattga ctgctgaaga
     2461 catcgcccgt gcagaaggtg taatcgttgc agcagacaaa aaagtagaga tgaatcgctt
     2521 tgacggaaaa gaactgatca atcgtcctgt cagtgatggt atccgtaaac cagaagagct
     2581 gatcaatcta gcccttagtg gtactgcacc agtattccat ggtgatggaa aagaaagcag
     2641 ttcagaagaa ggaaatgcgg atggtactat cggacaaaga atttacaaag atttgatgaa
     2701 cggcgtatca cacatgttac ctttcgttat tggtggagga atcgctattg cgctttcttt
     2761 catgatcgac caatttattg gcgtgccaca tgatcaatta tcaaatctag ggaattacaa
     2821 tcaagctgct agctggttca accaaatcgg acaagctgca tttggtttca tgctgccggt
     2881 attagcagga tttattgctt caagtatcgg tgaccgccct ggtttgatcg ttggatttgc
     2941 tgctggtgca ttagctaata caggcggtgc aggattctta ggtgcactga ttggtggatt
     3001 cttagctggt tatgtcatcg tatttttacg taaattattc aagaacttac caaaatcatt
     3061 ggatggaatt aaaacgatcc tcttttatcc agtgtttggc ttactgatta ctggtttctt
     3121 gatgttgctg gtcaatgtcc ctatgaaagc aatcaatgat gcattgaacg gtttcttgac
     3181 aagtatgagt ggttcgaatg ctgctttact tggtgcatta ttagctggaa tgatggcggc
     3241 agatttaggc ggtccaatca acaaagccgc ttatgtgttt ggtacagcaa ctttagctac
     3301 gactgttgca actggcggaa gtgtcgtgat ggcttctgtt atggctggag gaatggtacc
     3361 tccattggca attttcgttg ctacacgttt gtttaagaat aaattcagca aaacagatca
     3421 agatgctggt ttgacgaaca tcgtgatggg actatcgttt gtcacggaag gatcgattcc
     3481 atttgcagca gctgacccga ttcgtgccat tccaagtttt attattggtt ctgctttaac
     3541 tggcggatta gtcggagctt tcgggatcaa attactggct cctcatggcg ggattttcgt
     3601 tgtattctta ctgagtcacc cggtcatgta tttggtgttt attgctatcg gagcagtcgt
     3661 atctggtgtg atttatggtt tattgagaaa agctcctgca gcagcagaag catagaaata
     3721 aaaaagaaga ggctggtttt tcccagtctc ttcttttttt atagaatcat atagaatcaa
     3781 gtagtgtaga tgaatagttg ttgtaaaata gtttttccat gttcgttaaa atgattgctt
     3841 caaatttgtg atagaagatt tagcctcgat ttcctgcttg attcttggca agtttgcttc
     3901 atcagtcaag ataaccaaaa tatctcctac attcatgatc gtatcaccgt gagtcaagat
     3961 ttcctgattt cctctgcgta tagagattag cagcatattt ttaggccaat tgaagtctcg
     4021 taccattaat ccgtctaaag aactctctgc cgttactgga aaatcaaaag ttgtccttgc
     4081 accttttagt gaggcggtct gagtatttcc aagtagacgt tccaataatg attcgtaaat
     4141 tggattaccg ccgagaaggt cattgacgat ataagcaaca agagcagcca atcctaaagg
     4201 catcagatga ttaagagagc caaccatttc tgtcactaaa ataattgctg tcagcggtgc
     4261 ttttccaatc gctgtgaaat aaccagccat ggcaataacg acaaaatcct tgatgtagat
     4321 tggatccatc ccgaaatcaa ccataatgtt tccataaaag ctaccgatta aagcccccaa
     4381 tgtcaatatc ggaaggaaaa tcccccctgg aagaccacca ccgtaagaaa tcatggaaaa
     4441 aacgaatcga agaacaaaca aaccgataat cataatgatt ggcaaggaag catgtccaaa
     4501 agctaaaacg atttgattgc ctccgcctag agtactaggc cataaataac caattggcaa
     4561 aatcagcaag aaaggaacga acccgtgaaa ataagctggt aaaggcaatc ggttgtaaaa
     4621 ttttggcaaa gccagcaaga ccttctgata aataaaacct aagatgccta gaagtactcc
     4681 taagatcact aaggaaccat aatatttcaa cggcaaagaa ggaaggtcac ctagatataa
     4741 aacaggtctc aagccgaaaa agtaaagaga gacgaagtta gcagtgattg ctgaagcaaa
     4801 tgatgtcagc caaacaagtg gagaaaagga gtgatggatt tcttctaaaa caaagagtaa
     4861 tcctgcgata ggagcattga atgctgctgc aagtccgcta cttgcaccgc tagaaattaa
     4921 aatcttttct tctgaagatg gactccgaaa caattgtcca gctccttgtc caatggatgc
     4981 gccaagctgt atagaaggtc cttctctacc tagaaaaagg cccgcaccaa ctgaaagtac
     5041 accgccaatg aactttttcc ataaaacaga gaaccagttc attgataatt ccccgcgaag
     5101 ctgtccctct acttgtggaa tcccgcttcc cttgatatcc ggttcatttt tgaccagata
     5161 acccacgaaa aatgctgcaa ggatgcagca aatagctagg ccaattaagt aaatcggctg
     5221 ttcccgactc atttcataga tacttcgaac taaatcgctt agcttttcga tagataatcg
     5281 aaacaggcta acgacgattc ctgtcagcag tccaacaaat attcctttta gaataaaata
     5341 tatctgagtc ttatctaatt tcttgatttt tccttttttt tccattcaaa cgcctcgctt
     5401 tcttttaatt tagcgcgaaa tcaacgaatt gacaaggaaa aatccataaa aagaaacaaa
     5461 tgattttgaa ctagctagtt taatgaaagc caagaaaatg ctataattaa caagattgat
     5521 aaaagaaagg atgttgttaa tgtcatccaa agctgaatta ttaaacggga tgaacccccg
     5581 tcagaaagaa gctgttcttc acactgacgg accattactg ctaatggcag gtgcaggaag
     5641 tggaaagacg agagtgctga cacaccgcat tgcctatttg attgaggaaa aagaagtcaa
     5701 tccttggaac atcctagcga ttacttttac caataaggct gcaaaagaaa tgaaagaacg
     5761 tgtcaatgcg attctcgcat ctggaggcga agatgtctgg gtatcgactt ttcactcgat
     5821 gtgtgtgcgt attttaagaa gagatgttga ttttatcggg tataaccgga actttacaat
     5881 catcgattct tctgaacagc tgaccttgat gaaacggata ctaaaagaac tgaacattga
     5941 tccgaaaaaa tatgatcctc gaagtatatt aggaacaatc tctcaagcaa aaaacagcct
     6001 gcagacccct caggattttg caaaaatgca aggcagttat tacgaagaga ttgcggcaaa
     6061 atgttatgct gcttaccaaa aagagcttca atataatcaa tgtatggact ttgacgacct
     6121 gatcatgaat acgattcgat tatttgaaga acatcctgat tcactcacat attatcaaaa
     6181 caaattccat tatattcatg tagatgagta ccaagataca aatcacgcac agtatacgct
     6241 agtcaattta ttggcaggta gatttaggaa cctgtgcgtc gtgggagatg cagatcaaag
     6301 tatttacggc tggcgcggtg cagatatgca gaacatcttg gattttgaaa aagattatcc
     6361 agatgctgca gtaattttgc tagaacaaaa ctatcgctcc acgaaaaaca tccttagcgc
     6421 agcaaaccaa gtcattgaaa acaatagcaa tcgaaaacca aaaaacttgt ggacagaaaa
     6481 caaagaagga aacaagatca cttactaccg tgcagataat gaacgagacg aaacccgttt
     6541 tatcgtagat cggatgcaag aagagattcg aagcaatcat cggaattacg gcgactttgc
     6601 catcttgtac cggacgaacg cacaatctcg tgtaatggaa gaaactctgt tgaaagcaaa
     6661 tattccttat aaaatggttg gagggcacaa gttctacgat cgtaaagaaa tcaaagatat
     6721 tttagcgtat ttgaatgtgc tggcaaatcc tcaagattca atcagttttg agcgtatcgt
     6781 taattcgccc aaacgaggca tcggaccagg atctatcgaa aaactacgca gttttgcttc
     6841 tcttcatgaa tggccactgc ttgaagcagc gcaaaatgtg gatctggcaa atatcggtgg
     6901 aaaagcagga cagcaattag gtgcatttgg agagatgatc caagaagtca ctcagatgat
     6961 cccatacttg acagtgacag aattgacgaa agaggttttg gatcgtagcg gatatctaga
     7021 agacttaaaa atccaaaata cattagaagc acaagcacgc atcgaaaact tggaggaatt
     7081 tttaacggtc acacaagaat tcgacaaaca attcgaacag caaaatgaag aagacgctga
     7141 tgcaccagaa gaaaaactaa cggtcttctt aaatgatctg gcacttgttt ccgatattga
     7201 caatttagaa gaagatgctt cccaagtgac attgatgact ttgcatgcag ccaaaggatt
     7261 agaattccca gttgtattcc tgattggtct agaagagggg gtttttcctt tatctcgagc
     7321 attgatggaa gaaagcgaac tggaagaaga acgccgtctc gcatatgtgg ggatcacgcg
     7381 agcagaagaa gcgttgtatt taacaaatgc tttctccaga acattatatg gacggacgca
     7441 atataaccga ccttctcgtt ttgtagaaga aatcgatcag gagttactgg aaatcgaagg
     7501 catgcgtcct acgcctaaaa aaacacctgt attcgctaaa aaaacagcat atagctataa
     7561 acagccagag acagcggttg ttccctctaa aagtgctact ggaggggaaa ataacagctg
     7621 gaaacctgga gataaagtca aacacaaaaa atggggtcta ggaacagtcg tccgagtaag
     7681 cgggacgtcg aaggatctag aactagatgt ggctttccca agtcaaggag taaaacgtct
     7741 tttggctgcc tttgcgccaa ttgaaaaagc ttagtagtgg agggtaaatg atggaacatg
     7801 aaatgacgat tgacgaagct gctaaacgag cagaagaact tcgtacaaga ctgaaccaat
     7861 ggtctcgcga atactacgta gaagataaac caacagtgga agactatgtt tatgataaag
     7921 aatacgcaga attagtagca attgaggaac aatatcctga tttgatcacg tctgattctc
     7981 ccactcaacg agtaggcgga aaagtattag aaggttttga aaaagtcact catgatattc
     8041 cgctttacag tttgaacgac gttttcagca aagaagagct gatagctttt gatcaacgtg
     8101 tacaaaaagc agtgggacgt gtagttgact attgctgtga actaaaaata gatggacttt
     8161 ccgtttcttt gcgttatgaa gatggaaatt tcgtgagagg tgcgactcgc ggggatggaa
     8221 ctgtaggaga aaacatcact gaaaacctaa aaactgtccg gtcggtccca atcaaattaa
     8281 aagaaccaat gaacatcgaa gtaagaggcg aatgtttcat gccaaaacgg tcatttgtcc
     8341 aattgaacca agacagagaa gcagagggaa aagatatctt tgcgaatcct cggaacgccg
     8401 cagctggttc tttgcgtcag ttagattcta aaatcacagc gaaaagaaat ctggacactt
     8461 ttttatacac agtagctgat ttcggtccta tgcaagcaaa aacccagtat gatgcgctag
     8521 aagagctaga aaaaattgga ttccacacga atcgggaaaa acgtctatgt cattcgattg
     8581 atgaagtgtg gtcatacatt gaagaatatc atgacaaacg tgtggatttg ccctatgaaa
     8641 tcgatggaat cgtcatcaaa gtgaatgaat tctcgctaca agatcaactt ggttttactg
     8701 tcaaagcacc gagatgggca gcagcttaca agtttccgcc agaagaagtt gaaacgctaa
     8761 tcgaaaacat cgaatggaca gtcggtcgaa caggggtagt aacgccaaca gctatcatga
     8821 caccagtccg agtagcagga acgacagtaa gccgtgccag cctgcataac ggtgattata
     8881 ttaagttgaa ggatatccgc ttgaaagata cagttctgat ttataaagcg ggagacatca
     8941 tcccagaagt ttcgcaagtc gtgttagata aacgacctaa agacagcgaa gaataccaat
     9001 tgcctacaca ttgtccagtt tgtggaagtg aacttgtcca tttggatgaa gaagtagcct
     9061 tacggtgcat caatcctaaa tgccctgctc aaatgaaaga aggtttgaac cactttgttt
     9121 caagaaatgc gatgaatatc gatgggttgg ggccgcgcgt actagaacag atgtatgata
     9181 aaaaactagt agcagatgtt gctgatcttt acaaattgac agaagaagag ttattgacgt
     9241 tggacaaaat aaaagaaaaa tcagcaaata atattttgac agcaatcgac aacagcaaag
     9301 ataattcggt cgagcggttg attttcgggt taggaatccg acatgtcgga gcgaaagcag
     9361 caaaaatttt agcagaacat ttcggtgatc tagaaacatt gagtaaaagt gactatgagt
     9421 cgattattgc gcttgatacg atcggtgata tcattgccga cagtgtagtc acttatttca
     9481 gtaacgaaga agtccatgaa ttgatgaatg aattaaaaca agcaggcgtc aatttcgaat
     9541 acaaagggct tcgaaatgcc caattgcaag aagtcgagtc tccttttaaa gaaaaaacag
     9601 tagtccttac tggtaaacta acacgattta ctcgtgaaga agcaaaagag acgatcgaaa
     9661 atcttggcgg gaaagtgact ggtagcgtat cgaaaaaaac ggatatcgtg gtagcaggag
     9721 aagatgctgg cagcaagctg accaaagcac aagaattagg aatcgaagtt tggacagaag
     9781 atcaaatggc agatgcattg gctaaaagtc gctcagtaga agaatgaaaa atgagttttt
     9841 tcagacaaat ttcaataaac aatttcagac acggcactct tgtgttaaaa tagagagcaa
     9901 agtttttaac tcgtttagaa agaaggggta gtatggcgat tagtgaagaa caagtgaaac
     9961 acgttgccaa attagcaaaa cttgcgttcg cagatgaaga acttgcaagt tttaccgacc
    10021 aattaggtaa aatcatcgat atggttgaaa tgctggaaga agtagataca gaaggtgttc
    10081 catttacgtc gaatgtggtt gaaacagtaa acgtgatgcg acaagatcgg gcgcaagctg
    10141 gatggaaccg cgatgaatta ttgaaaaatg tgccggaaac agaagacggc tttatcaaag
    10201 tacccgcaat catcgataat ggggaggctg gcgcataatg acagaattac acaatcgttc
    10261 tctagaagaa ctgcatcata tgcttgtatc gaaagaaatc actgtacaag atttgacaaa
    10321 agcaacattt gaacgaatca aagaaacaga gcctgaggtc gaagcattca ttacattgaa
    10381 cgaagaaaaa gcattggaac aggcaaaagc tttagatgaa aaagggattg atgaaagcaa
    10441 cgtccttgct ggtattccaa ttggtatcaa ggataatatc gtaacaaaag atatcttgac
    10501 aaccgctgca tctaagatcc tttacaattt tgaaccaatt tatgatgcaa cggtcatgga
    10561 taaaatctat caagcagata tgatccctgt tggtaagcta aacatggatg aatttgctat
    10621 gggtggaagt acagaaactt cttatttcaa gaaaacaaaa aatgcttggg acccaacaaa
    10681 agtacctggt ggctcttctg gtggttctgc agcagcagtt gcagctggac aaattccagt
    10741 ttctttaggt agtgatactg ggggaagtat ccgccaacca gcagctttta acggcatcgt
    10801 agggatgaaa cctacttatg gccgtgtctc acgttttggt ttgattgcct ttgcttcatc
    10861 tcttgaccaa atcggaccaa tgacccgtac ggtgaaagat aatgcattag cattgacagc
    10921 tatcagcggt tatgacgaaa aagacggtac ttcttcaggc gtttcagttc ctgattttgc
    10981 agagggactg acaggcgaca tcaaaggaat gaagattgct ttacctaaag aatacttagg
    11041 cgaaggtgtg gcccctggcg taaaagaagc tgtattgaaa gcagcagaaa cattccgttc
    11101 attaggtgct gttgtggaag aagtcagctt gcctcattca aaatacggtg tggctgttta
    11161 ttatatcatt gcctcatcag aagcaagttc caacttgcaa cgattcgatg gtatccgcta
    11221 cggttatcgc tcagaaaatg tgaaatcatt agatgatgta tatgtcaact cacgttcaga
    11281 aggattcggt gaagaagtaa aacgccgtat catgttaggt acattctcct taagtgctgg
    11341 ttattatgac gcttacttca aaaaagcggg acaagtccgt acattgatca aacgagattt
    11401 tgaaaaagtc tttgcagatt atgacttgat catcgggcca tcttcaccaa cagttgcatt
    11461 cggaatcggt gagaacatta acgatccaat cacaatgtac atgagtgaca tcttaacgat
    11521 ccctgtcaac ttggctggac ttccaggtat gtctgtaccg gcaggtttat cagaaggatt
    11581 accagttgga ttgcagatca tcggtaatta ttttgatgaa aaaacgatgt atcaagcggc
    11641 atatgcgttt gaacaagcaa cagaattcca taaacaacaa ccagctattt taggagggaa
    11701 agacgaatga attttgaaac agtcattgga ctagaagtcc acgtcgagtt aaaaaccaac
    11761 tcaaaaattt tctctccagc gccagctcat tttggtgcag aaccaaacag caatacaaac
    11821 gtgatcgact ggggttatcc aggcgtgctg ccagtaatga ataaagctgc gctggaattc
    11881 gggatgaaag cagctttggc attaaactgt gaaatctcta aagatactca ttttgaccgg
    11941 aaaaattact tttacccaga taatccaaaa gcatatcaaa tttcccaatt tgatcaacca
    12001 atcggtcatg acggatggat cgaaatcgaa gtagaaggta aaaagaaaaa aatccggatc
    12061 gaacgtgtcc atttagaaga agatgctgga aaaaacatgc atggtattgg cggttattct
    12121 tacgttgatt tgaaccgaca aggcacgcct ttgatcgaaa tcgtttctga agctgatatg
    12181 cgttcaccag aagaagcata cgcttatcta gaagcattgc gatcaattat ccaatttaca
    12241 gaagtcagcg atgtgaaaat ggaagaaggc tctatgcgct gtgatgccaa tatttctctt
    12301 cgcccttatg gacaagaaga gttcggtacg aaagcagaat tgaaaaactt gaactcttta
    12361 tctttcgtga aaaaaggttt agcttttgaa gaaaaacgtc aagcaaaagt tcttctttca
    12421 ggaggagaaa tccaacaaga aacacgccgt tttgacgaaa caacgaataa aacagttttg
    12481 atgcgtgtca aagaagggtc tagtgattat cgttacttcc cagagcctga tatcccgaaa
    12541 ttcgtgatcg atgacgaatg gattcaaaaa gtaaaagcta gtttgcctga gatgccagct
    12601 tctcgtcgcg aacgctatat ccgtgagttt ggacttccag agtatgacgc aatggtattg
    12661 acacttacaa aagaaatgtc tgattttttt gaagctgttt tggaaaatgg tgcagatgcc
    12721 aagcaagcat ctaactggtt gatgggtgaa gtttctgctt acttgaacag tgagaaactg
    12781 gaattagctg agacaaaatt gacacctgag aacttagcag gaatgattcg tttgatcgcc
    12841 gatggaacga tcagctcaaa aatcgccaaa aaagtcttcc gtgaattgat cacaaatggc
    12901 ggagaagccc aagaagtagt tgaaaaaaat ggttgggtgc aattatctga tccgaacaaa
    12961 ctactgccaa tcattaatga aattttggat aacaaccaac aatctgtcga tgatttcaaa
    13021 aatggaaaag atcgtgcggt tggcttccta gtcggtcaaa tcatgaaagc aactaaagga
    13081 caagcaaatc ctggtgtcgt gaataagcta cttaaagatg aattagcgaa acgatagagg
    13141 ctgaaaatga aaaaagcacg cgtaatttat aaccccacct cgggaaaaga attattgaaa
    13201 aaaaatttgg cggatatcct acaggcatta gaagaagcag gatttgaagc cagcgcctat
    13261 gcaactacac cagaaccaga ttcagccaag aatgaagcat gtagagtcgc agaactaggt
    13321 tttgatctag tagtggcagc tggtggcgat ggcacaatca acgaagtggt taatggaatc
    13381 gctccgcttg aacaccgtcc taaaatggcc atcatcccag ctggtacaac gaacgactat
    13441 gctcgagcat taaaaattcc tagagataat attaaagcag cagcagaagt gataaaaaaa
    13501 caccaaaccg taaaaatgga catcggcctt tctgataaca gttacttcat caatatcgct
    13561 gcaggtggct atttgacaga attgacttat gaagttccat cagaactaaa gagtattttt
    13621 ggttatcttg cttatcttgc gaaaggagca gagatgctcc ctcgagtaaa gccaatcaaa
    13681 atgcgcttga agtatgatga aggggaatat atcggaaatg cttcgatgtt tttcctaggg
    13741 ctgacgaact ctgttggagg attcgaacag atcgcgccag atgccaaatt ggatgatggg
    13801 aagttttctt taatcattgt gaagacagca aatatctttg agatcttgca tattgcagca
    13861 ctcatgctga atggcggtcg acatgtcgat gatccaagaa taatctatac caaaacaagc
    13921 agtttgttcg ttgaaacaga agatgatatc aatcgaccag taatgatcaa cttagatggt
    13981 gaatacggcg gggaagcacc gatgcatttc caaaatcttc atcaacacat tgagttcttt
    14041 gccaataccg atcagattcc tgatgaagca atcacaggta cagatgaaga agaattagag
    14101 gttgtcagca aagaattcgt aaaagaagtc gaacgtttaa cggatgaaga tatcgacggt
    14161 gacggaaaaa tagcagacga aacacctaat caaaagaatt aatcaaaagc cggtgctgaa
    14221 cgtagtaagg tcagtgccgg ttttagttgt gtaattagct gatttatagt agagttcagt
    14281 aggattgaaa aataggctta aaggagcaaa atatgacaga acagcaaata gtaaagaaaa
    14341 acgaacgata tacagtcgaa atcgtcgact tgagttatga aggaatgggg gtagcaaaaa
    14401 tcgatggtta tccattattt atcgaaaatg cactccctgg agaagaagta gaaatactag
    14461 tagtaaaagc aggcaataag tttggatatg gaaaagtcga aacatttatt acacaatcac
    14521 ctgatcgtca gcctgtagaa aacaatgcat tacttcgcat aggtatcgca cctttagccc
    14581 atttgaagta tgaacaacag cttgtcttca aacaaaaaca agtagagcat gtaatggcga
    14641 agattgctaa gatgccagaa gttcctgtac ttccaacggt cggaatggca gaaccattcg
    14701 gctaccgtaa taaagcacag atccctgttc gccgtatcga tggacagtta gccaccggtt
    14761 tttacaaaaa gaatagccat gatctagtcc ctatcgaaga tttttatatc caagatcctg
    14821 tcatcgatca agcaatcaaa gtagtcagag atatcttgca acggttccaa gtacgaggat
    14881 ataatgaaga aaaaaatgaa ggacagcttc gtcacatcat tatccgtcgt ggtcattatt
    14941 cccatgaaat gatggtcgtt ttagttactc gtaaagaaaa attctttaaa tccaaggaaa
    15001 ttgctgaagc tattcatgaa gaattgccag aagtggtttc ggtcatccaa aatatcaatg
    15061 aagaaaaaac aaatgtgatt ttaggagaca aagaaaaagt tttatatggc agagactata
    15121 tcgaagatca gctattagga aaaacctatc gaatctcagc aaagtcattt tatcaagtaa
    15181 atactcaaca agcagagtat ctgtaccaaa cagccatcga gtttgccgag ttagataaaa
    15241 aagacattgt gatcgacgct tattcaggaa taggtacgat tggcttgtct ttagcagaca
    15301 gagtggataa ggtgtatggg atggaagtta ttccagaagc aattgaagat gcacagttca
    15361 atgctttaaa taataaaatc gaaaatgttc attatgaagt cggaaaagca gaaaaagtta
    15421 tgaaaaaatg gcaagaaaaa gggatcaaac catctgtgat cgtagttgac cctccacgaa
    15481 aaggattaga tcatcatttt attgaatcag caatcgaaat gtcaccagaa cgaatcgtct
    15541 atatctcatg taatccggca acttttgcaa gagatgctaa gttatttgca gaagcaggtt
    15601 atgccgtgaa gaaagttcag cctgtagatc ttttcccgca aacccatcat atcgaattag
    15661 tcgcttcatt cgttaaagga gaataaaaat ggaccaaaaa gaaaaaatga aagaattgat
    15721 caaaaagaaa caaggtggag gccgatccaa acaaatggaa acacctaaga atgatcgtaa
    15781 aaacatgaga aaaggcccta aaatctttaa taaatagacg tgctaaagaa agactaagaa
    15841 gtgttaactt ttgttattgt ttcttagtct ttttagtggc tctttgtcaa taaggactga
    15901 tgggttgtac aaaatcaaat ctgggtcaga atgaacgtca ttctgtctca gatttttcgt
    15961 tatttcaatt tgattaactg attgatcaaa aagattttta ttttcatatt ctcaaatgat
    16021 tcaacactaa atatacatgt ttgacgcgga aattattatt cgattttggc ggcatcaggg
    16081 gggatgacga cgtagcagac ccattatttt actcaagaaa atgtccgtgc ctattattta
    16141 gaatacgata gtgaacgtgc tgggagtttt gttcttttag agaaagtctc tgaagataaa
    16201 ttagttgtct tagattttga ctgtaacgaa aaatggcgaa atggaagagc accagcaagt
    16261 gattgaccgg atctacgaag cgacacgata tctgcctttg gatcgtttgt gcttaagtcc
    16321 tcaatgtgga tttgcttcaa cagaagaaga aaatgttttg tttgaagaac aatggaaaaa
    16381 atttcttttg tcaaaagcat tgcagaagaa gtttggggaa aatcattaaa tcatctagga
    16441 gagcttgata ggaaataaca ctatggtata atcagaatgc ttaatactaa ctactgaggg
    16501 agccttatga aaaagaaaat tcttaaattt atcggatggt tcttcggaat cattcttggg
    16561 atcgcgtttg caatctttct tgccttccag ctttccccca gacctggagc attcttgatc
    16621 aaccatatgt tttcaaatac tgttcaaatc actgataaaa aaacgttcga tcaagcagaa
    16681 aaaaacgtgg cgaaaaaaac agatttgacg tatgagtcca agtataaaga gaatacttat
    16741 gacatctact atccaaaaaa cagcaagggg cctgttcctg ttctattttg ggtacatggt
    16801 ggaggattcg tcggcggaga taaatcgggt gtcaaagaat ttgcgacaaa attagtttca
    16861 gacgccaaca tcgcagtggt tgcgatgaat tatgaattag cgcctgattc agaataccct
    16921 aatcaagttc ttcaagtgaa tgaactgatc aaagatttat tagctgaaga aaaagacgac
    16981 aaattgctag acatggaaca attatttttt ggtggagata gtgcaggttc acaaatcgca
    17041 ttgcaatatg cagctataca aacgaatgat gcgtatgcta aacaaatgaa aatcaaacag
    17101 ctacttccaa aagatagtct gaaaggaacc atttcttatt gcggacctgt tgatttgaag
    17161 ctaacagcac agcagcattc ggatgatcga tttatgaagt ttttcgtcaa aaccgttgcg
    17221 tggtcgttga ttggaacaaa agattggaaa acgagtgagc aattgaaaga agctagtgtg
    17281 gctgaccatg tttctaagga atttccgcca acgtatataa ctgacggaaa tgcatactca
    17341 ttccaggagc aaggtcaagc attagaaaat cggttgacag aattaaaggt acccgtgcaa
    17401 tccttgtttt atacagatac aaaaaaagag attacccatg aataccaatt tgattacacg
    17461 ctaaaagaat cgcaaaattg ttaccagcag acattggatt ttgtgaataa gtataagtaa
    17521 gaggttgtga aaaagccgtt tcgttttgag tatgaagaaa cgaagcggct tttcacaacc
    17581 tctctttcaa tacctttttg ttcttgggat aaaactttcc ctaacaatca atcgtgcagg
    17641 ataagtcaaa atgattggtt cgtcccgtat accgtcaatt cgctccaatg ccattcgaac
    17701 agctgcttgc ccaattgatt tcgcattgat ttgaacactg gtcaagccag gggagaggtt
    17761 tgcgattgtc tcgatattat caaagcttgc tacaacaatc tcttctccaa tttggatatt
    17821 tttttgtttc aatgcccgat agacaccaat tgccattgga tcgctcccga caagaatggc
    17881 agtaggaaga ggggattcat tttctaatag attagtcgca agtacctcac cttgtttttc
    17941 tgtccagcca cctagatgat aagacatata tttttctaag tgatgatcga tcataaaatg
    18001 ctgataggca cgcatcctct tttcatttat agaaatctgc ttcccaccga attcatccat
    18061 atcgacttta tatcccccga tataggctat tcttttatga ccattttcta agaacaattc
    18121 cagtacggtt ttagtcatcc gttcaagatc tgcatacacc atatcgacct cttgctggat
    18181 gtcagggtta tccacgacga ctatttgcat gttttgtttt aacaaggcgg taatcgattc
    18241 tttcgtaaca gtcccaacaa cgatcaccgc acctaactga tctagatctt cccattgttt
    18301 aggattatca gatagattat agattcgatt catccctaaa tgcagacgtc gagcttcttc
    18361 ttccatccct actcgcaaat gtttgtaata gatatcctgt tcttcgctcg ctttgccgat
    18421 cgtagagatc acaccaatcg aaggcattct ctttttgata tatttacgag tactgatgtc
    18481 ataatccaac gcagcagctg cctctactat ccgttgccga gtagtctgag ccacatggat
    18541 cgtctgatcg ttattcaaca cacgggaaac tgtagcagga gagacatttg cctttcgtgc
    18601 gacatctcta attccaac
//
LOCUS       DAGCWU010000044        18288 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.044, whole genome shotgun sequence.
ACCESSION   DAGCWU010000044 DAGCWU010000000
VERSION     DAGCWU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.044"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            42..197
                     /locus_tag="MBZ71_001198"
     CDS             42..197
                     /locus_tag="MBZ71_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429164.1"
                     /translation="MVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLLNL
                     FNRIVGA"
     gene            210..713
                     /locus_tag="MBZ71_001199"
     CDS             210..713
                     /locus_tag="MBZ71_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBT4429165.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            840..1877
                     /locus_tag="MBZ71_001200"
     CDS             840..1877
                     /locus_tag="MBZ71_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429166.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1892..3589
                     /locus_tag="MBZ71_001201"
     CDS             1892..3589
                     /locus_tag="MBZ71_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HBT4429167.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3582..4043
                     /locus_tag="MBZ71_001202"
     CDS             3582..4043
                     /locus_tag="MBZ71_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HBT4429168.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            4056..4916
                     /locus_tag="MBZ71_001203"
     CDS             4056..4916
                     /locus_tag="MBZ71_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429169.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4954..5343
                     /locus_tag="MBZ71_001204"
     CDS             4954..5343
                     /locus_tag="MBZ71_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4429170.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5327..7777
                     /locus_tag="MBZ71_001205"
     CDS             5327..7777
                     /locus_tag="MBZ71_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4429171.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            7782..9890
                     /locus_tag="MBZ71_001206"
     CDS             7782..9890
                     /locus_tag="MBZ71_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4429172.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9887..10891
                     /locus_tag="MBZ71_001207"
     CDS             9887..10891
                     /locus_tag="MBZ71_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBT4429173.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10910..11821
                     /locus_tag="MBZ71_001208"
     CDS             10910..11821
                     /locus_tag="MBZ71_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4429174.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            12008..12295
                     /locus_tag="MBZ71_001209"
                     /pseudo
     CDS             12008..12295
                     /locus_tag="MBZ71_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12483..12698)
                     /locus_tag="MBZ71_001210"
     CDS             complement(12483..12698)
                     /locus_tag="MBZ71_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429175.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12957..13124)
                     /locus_tag="MBZ71_001211"
     CDS             complement(12957..13124)
                     /locus_tag="MBZ71_001211"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4429176.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(13469..14908)
                     /locus_tag="MBZ71_001212"
                     /pseudo
     CDS             complement(13469..14908)
                     /locus_tag="MBZ71_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15456..16127)
                     /locus_tag="MBZ71_001213"
     CDS             complement(15456..16127)
                     /locus_tag="MBZ71_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBT4429177.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16476..17927)
                     /locus_tag="MBZ71_001214"
     CDS             complement(16476..17927)
                     /locus_tag="MBZ71_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBT4429178.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(18039..>18288)
                     /locus_tag="MBZ71_001215"
     CDS             complement(18039..>18288)
                     /locus_tag="MBZ71_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HBT4429179.1"
                     /translation="VFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAI
                     EEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         5954 a         3064 c         3517 g         5753 t
ORIGIN      
        1 aatggttttt aaccaatgct caatctttag tcttactagc gattgtggtc attggtttgt
       61 ttttaggatt taaacgggag ttttccaaac tgattggctt cttggtgatt gctttgattg
      121 cggtaggtct ggtctttaat gcttctggtg tgaaggatgt cctactcaat ttgtttaacc
      181 gaattgtcgg tgcctgaggg ggaatcacaa tggaacagat gcgtgtttat attgcgaacc
      241 ttggaaaata caatgaaggt gagttggtgg gggcatggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacagatt ggattaaatg acgaatatga agagtacgcg attcatgatt
      361 atgaactgcc ttttgagatt gatgagtata cgccaattga agagattaat cggttgtgta
      421 acttagcgga ggaactagaa ggaacgccaa ttggtgaagc tgcttctgag attcaacacg
      481 ctttcttcaa ttctttcgag gaaatggtag agcatgtgga tgatattgtc tattatccag
      541 attgcgatga tatgagtgat gtggcgcatt acttgattgt agaagctggt gattttggcg
      601 aagttccaga acaattgaag ccgtatattg attttgatgc ttgcggtcga gatttggaga
      661 ttggaggaaa ttatttggtt actagtcgtg gagtttttga gtatccagaa tgaatcaaac
      721 actatatata gtgtttgatt agtatatgta agcaaaaaaa cacaatatat agagtgcaaa
      781 ctgaagggta atgtgatata atatttgccg aagaaatatt tttgaaatga ggtaatatta
      841 tggcaaatat tcaaacaagt tttattgatt tccataattc aattagattg gacgtagaag
      901 acaatacgct acttaaggac tataaagacc aagtaattga tggattaaaa gattacttac
      961 ctgatgatgt gaagtttgaa acttttttac agggaagcta ttctgtttat accggaataa
     1021 aaagctgtga tgaaaaaatt gattttgaca tcgatattgc agttgctttc gaaattgatc
     1081 atactgttta tgaagatcca agggagccta aactgtgggt aaaagaggcg ctagttgaaa
     1141 tttttcctaa tgctcaagtt aatttaaaag taccttgtgt aactgctaca tttactggaa
     1201 aaaagacaaa aaaaaatgtg cacgttgatg ttgcggttta tgctaaagaa gatgaaaatt
     1261 atttcttagc aaaagcaaaa gaattcagtg ctcccgaaaa ccgatgctgg gaggaagcgg
     1321 atcccaaagt attaaaagag aaaattaata gtcatgtagc agattcagat gatcgaaaac
     1381 aatttcgaag atgtattcgc tatttaaaaa gatggaaaga taacaatttt aaccaagaat
     1441 ataaaccaac aggaattgga cttacaatta atgtgatgga tacgttccta gttaataagt
     1501 ccactgattt tttaactaga aaagttcagt ataatgatat ggaatgtatg aagcagattg
     1561 tttcttcgct gaaagattca tttgtgtatg agtacagtga aacagatggt tggcattatc
     1621 gtctacatgc atatcttcca gtgaagccta acagtgacac ctattcaaaa atgacagtga
     1681 atcagatgag tgattttaaa aacaaacttt ctaaattata tgatgatttg attttcgcta
     1741 tcgatactga agatgagtat gaagctacaa aacgattaaa taatcaattt ggggaggatt
     1801 ttttaattat ttcagaagaa gaagttacag aaaaaaactt aagaaatgca tttgttacag
     1861 actatccgag tgcttaggtt ggtgttaaag attggattat actgatattt taaaagaaaa
     1921 gctaacagat gttgtgcgtt tattcgatgg taattactat ggggattttt catttgctac
     1981 aattggaaag ttgcgtttaa aaattgaaat accaaaacat tttccattgg tacttcctag
     2041 gatttacatt cataattttg atcatttagg actttaccta ccacatgtag aaaaagatgg
     2101 gttggtatgt ttttcttcag atataaatta tttagtagat gaagaaaatt cagaattggt
     2161 tttattgcag tgtgttaata aaactataaa aacaattgag gaaggtttga aagggttaaa
     2221 taaaaatgat tttcgaaaag agtttgttgc tttttgggac aaacaagaga atcaaatttc
     2281 gttattctac ctttgtgaac cagcgttgtt tatcaagtca gttgatacaa taatgtacgg
     2341 taagtctttg gttgctttgg ataaagagaa cgaattacaa ttaaatgcag cgagtagatt
     2401 gtttaatgga gagaaagaag atactcatga attagatgca tactatgttc caattagaaa
     2461 caagaataat attatgcccc cgaagaataa taaagcatgg tcaaagaagg atattttaca
     2521 aatagtaaaa aaaaatgtaa caggtgcaaa taaaagattt tttttaaaac ttgaaaaaag
     2581 aaagaataga cataatgttt tacttattct agatatccca attgaagaaa agtcaaataa
     2641 tgtcataatt ggatttcttt ttacaaaaca atctggatct ataaaagatt ataagggtac
     2701 gcctaggact gtattagttc agcgtcatga tgaattatat ttggtgaata gaacgagcgg
     2761 agaaaactat ggattgggtg ctaatatagc tattgttggg gtagggtcag ttggcagtaa
     2821 aattgctgaa aatcttggtc cattaggtgt gagaaagatg acactcatag acaatgattg
     2881 ttttcaagct gaaaatttgt atcgaaattc acttggtgca gatagtttaa aaaatgcccg
     2941 atctcagaat aattttttta aagttgattt aatgcaggag gagctcgaaa aaaaattccc
     3001 atacctttgt gttgaagtgg agtcaatgaa cgttttagac ttaattagta tgcaacctgg
     3061 ttttttcaag gaatatgact atgtttttgt ttgcatagga gatacaatgg caagcctggc
     3121 tttaaataat tatttttcca aaagtagtca aaaagttttc tattcttggc tggagccata
     3181 tggcattggg ggacacactc tatttataaa ttattctcta aatcaaaaag gttgtttcaa
     3241 ttgtctttat acagattcaa actctaaaaa taggtgttca aatagagctt ctctggtaaa
     3301 agaaggacaa atttttgaaa ggactttagc gagttgccgc tcaaaattta taccatataa
     3361 tgctattgcc tcgacggaat gtgcaattaa tgcaataaaa cttttccata aagtcaatca
     3421 aaataatatg accaatagta ctcttttttc atggatagga gatgggagta cttttgagaa
     3481 tttaggatat caatatagcg acagaaatac ttttcttaat ggagtcaata agtttgaaga
     3541 aagattcttt tctaaatatt gcaaagtatg tggggagtta gatgattaaa ttttcgtgtg
     3601 accgtattgt cattgaagta agggatgaag ttcttaagat atttgaacaa tatcggcagg
     3661 gaaaggatga cttgcctgaa gctggaggag ttcttcatgg aaaaagactt aagaatggga
     3721 gcataataat taccgatata acaacacctc aaataggaga tatacaaaaa agattactct
     3781 ttaaaaaaaa ccgagaaatt catcaacagc tttctaatga gaaatggata gaatcaaata
     3841 agttttctat tgcattggga gaatggcata ctcatcctga agctgtacca actccatcaa
     3901 atgttgataa gaaaagctgg agactgaacg tgagcaagca acatgatgat agagtttatg
     3961 tttttattat cgttggattt tctgatttga gaatgtggtt actgtcaaaa aaatcagata
     4021 ttaggcaggc tataaaacta taaaggagtg attaggtgaa atcacaaaag aatatttggt
     4081 ggtgggggtt tttagtagga aaggtaatga taccagccct tctcttaatt tctctaccga
     4141 tgattacttt tttctttacc aataaaaaaa tgagtaaaga agccgtgatt tttttctttg
     4201 gagaccaaaa agctaccttt attgaaacgt tagtaacatc tcttcagctt aatttgaatt
     4261 acattttttc aattattatt gttatatcgt tattgaattt ttttaaaaaa agaaattcag
     4321 gaaaggtgtt taactctaat ggaaatgtgt actacaacta tttttatttt gtattttggg
     4381 tagcagcaac tttacttggt tatgataaaa ttcagattgc aggaattcct atacacatgc
     4441 aatataagtt ggttttgagt ggaatttttt ctgaggtgtt gccagatata tacgatgatc
     4501 attacgacag tgatggaaca tgtaaagtaa gcattgaaaa agaaaatttc gatgacatcg
     4561 atggatatga ctctgtgaac ttactgatta ttgatactta cgatataaaa atgagtgaat
     4621 tatctatgga aaatcaaaca tatcctacga taattgttag agggaattca atagatggag
     4681 ttcgtaaagt taatagatct ttaatcttgg aaataaaaaa aacaatggat gaaattcaaa
     4741 aaagtgactt taaaaaagta tttgttgctt caacatctaa tccaaaaaat agtatcaata
     4801 ttattaattc atcgtttaga tttttcggtc gatcacgtag gtttaaactt tatgttcttc
     4861 aaaaggatta tgcaagcaat ggaaagtata gtaagaaata tagaattttt atttaagaag
     4921 gaatttttcc ttctttttat tttggaggaa actatgaaaa aaataaaaag ctacaccagt
     4981 atttgggcag ttgaaaaagt catttatgcg attaatgact ttcagctgcc ttttcctgtg
     5041 acctttaacc agatggcttg gtttgtttta tccttgatgt ttgtcattgt atttgcccat
     5101 gtgcctccgc tatccatgat agagggggct tttttaaaat acttgggcat tccgatagcg
     5161 gtcacttggt tcatgtcgca aaagaccttt gacggtaaga aaccattggg atttttacga
     5221 tcctttatca gctatcactt acggtttaag gtcacttttg caggaaagaa agttaaagaa
     5281 cagaagaaac gatgggacga accaatcacc ttggtaagga gtgtgaatta tgtacccgat
     5341 taagtatatt gaaaataatc ttgtctttaa ccaagaaggc gagtgttttg cttactatga
     5401 attagtgcct tacaattact cctttttgtc acctgaacag aagtatcaag tacatgataa
     5461 ctttcgccaa ttaattgcgc aaaatcgtga aggtaagatc catgccttac aaattgccac
     5521 agaaagtagt attcgtgcca cacaagaacg ttcgaaaaag gaaattaccg gtagactcaa
     5581 agaagtagca aagcaacgaa ttgatttaca gacagatgca ttagtatcca tgattggcga
     5641 tagtcagatt gattatcgct tctttattgg ctttaaactg atttcgacag atgaagaggt
     5701 caatctaaag aatctcagga aatctttctt ttctggactt caagaatttg tctatggagt
     5761 gaatcatcat ttgatgggtg attttgtttc cttatccaat gaagaaattc gtcgctatac
     5821 caagcttgaa aaactaatgg aaagtaaatt ggctcgaaga tttaaagtca aacgagtaac
     5881 gccgagtgac ttgacgtatt taattgaaca tatctatggt gaaaaaggca caccgtttga
     5941 agaatatgaa ttccagctac caaagaaaaa gctgaaatca gaaacgctgg tgaaacgtta
     6001 cgacttatta cgtcccactc gttgtttgat tgaagaaaag cctcggtact tacggatgga
     6061 acatgaaaat catgagtctt atgtagcgta cttaacaatt aataccattg taggggaaat
     6121 ggagtttcct tcctcggaac ttttctatta ccagcaacaa caatttacgt ttccgattga
     6181 tacttcaatg aatgtggaaa ttgtgacgaa taaaaaggcc ctagctaccg ttcgtaataa
     6241 gaaaaaggaa ttaaaagatt tagacaacca tgcctatcaa tctgataatg aaacgaactc
     6301 caatgtatta gatgctttgg attccgtgga tgaattggaa accaccttgg atcagtcgaa
     6361 agaatccatg tataaactaa gctatgtggt tcgagtgagt gctaaatcag tggatgaatt
     6421 aaaacgccgc tgtgatgaag tgctcgattt ttatgacgat accaatgtaa aactagtccg
     6481 gccctttggc gatatgatgg gtctacacaa agaattctta ccctcaagta agcgatacat
     6541 gaatgactat attcaatatg ttacgtctga ctttttggcg ggacttggtt ttggggctac
     6601 tcaaatgcta ggagaattag aaggcattta ctttggctat aacgtagata cggggcgcaa
     6661 tgtttacctg aaaccggcat tggcatcaca aggggtcaaa ggctcagtaa ccaatgcttt
     6721 ggcatctgct ttccttggtt cgttaggtgg ggggaaatct tttagcaaca atctcttagt
     6781 ctattatgcc gtcctttttg gtgggcaagc tgtgattgtc gatccaaaag gagaaagagg
     6841 cggttggaaa gaaacgttgc cagaaattgc ggatgaaatt aatattctta acttgaccag
     6901 taagccagaa aatcaaggct tacttgatcc ctatgtgatt atgaagaaaa agaaagactc
     6961 agaaagtttg gcgattgata tcctgacctt tttaacaggg atttccagtc gggatgggga
     7021 gaagttccct gtgttacgca aagccattcg aacggttacc caaagtgatc agcgaggatt
     7081 attacaagta atcgttgaat tgcgtaaaga ggactcacca gtcgcggaaa atatcgcgga
     7141 tcacatcgag tcctttaccg attatgattt tgcccatttg cttttctcag atggttcgat
     7201 tacccagtcg attagtttag ataagcagtt aaatatcatt caagtagcgg atttggtttt
     7261 accagatgca gaaaccactt ttgaagaata taccaccatg gaattgctga gtgtttccat
     7321 gctaattgtt atttccactt ttgccttgga ctttatccat agcaaccgag aaattttcaa
     7381 gattgtggac ctggatgagg cttggagttt tcttcaagta gctcaaggaa aaacgttatc
     7441 gaataaatta gtgcgtgctg gtcgggccat gaatgccggt gtttacttcg tcacgcaaaa
     7501 cagtgatgac ttgttggatg aacgtttgaa gaataatatt gggctaaagt ttgcatttcg
     7561 cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggattag ataaagaaga
     7621 tgaaagtaat caaaaatgca tgcgagattt ggaaaatggg caatgcttga tgcaggattt
     7681 gtatggtcga gtaggtgtgg tccaagttca tccagtcttt gaagaacttt tccatgcttt
     7741 tgatacccgt ccgccagttc aagagagaat ggagtgagga gatgaaaaag aaactttttc
     7801 gtatctttgg aattgtttta atcagtttgg tcactctgat aatacttttg agcttagttg
     7861 gaacggtagc tgaggcaaca ggtctggttg atgatacagt tggagccgga aatctttatt
     7921 cccaatattc acttggtaac tatcagttgg atttctttgt ggatagttcg tgggattggc
     7981 tgccttggaa ttggggtgat ggcctaggga agagcgtaat gtatggactt tatgccatta
     8041 ctaattttat ttggacagtt agtctctact tatcaaatgc cacgggctac gtggttcaag
     8101 aagcctataa gctggatttc atttcagata cggctgaaag tattgggaag aatatccaaa
     8161 ccttagccgg cattaccgaa aatggactcc agacctcggg cttttacttt gggtttctat
     8221 tattgatgat tttagctcta ggagtttatg tggcgtatac aggattatta aaacgagaaa
     8281 ctaccaaagc agtccgggct gtcattaatt ttgtgatgat ttttctactt tcgggttcgt
     8341 tcattgcgta tgcgcccacc tatatcacaa aaatcaatga ttttagctca gatgtcagcg
     8401 aagcggctct tagccttgga accgagattg tcgtgccgaa ttcagaaagc caaggaaagg
     8461 acagtgtgga tttaatccga gatagcttgt tttccattca agtccaacaa ccgtggttac
     8521 tcttgcaatt tgatgattcc aatatagaag aagttggcga agatcgtgtt aacaaaatcc
     8581 tgtctgtaag tccagatgaa aataaaggca aggatcggga agaagcggtc aaagcggaga
     8641 ttgaagataa tgacaatgcg aatctcagta ttaccaagac catgaaccgc ttagggaccg
     8701 tggtcttttt ggtgttgttc aatattggca tatccttctt tgtctttctc ctaacaggga
     8761 ttatgttgtt ctcgcaaatt ctctttatta tctttgcgat gtttttgccg attagtttct
     8821 tattgtccat gttgccgact tatgagagtt tgggcaagaa ggcgattatt cgcttgttca
     8881 atacgattat gatgcgagca ggagttactt tggtgattac gactgcattt agcatctcca
     8941 cgatgttttt caatatttcg gcaacctatc catttttcat ggttgctttt ctgcaaattg
     9001 tgacctttgc cggtatctat ttcaaattgg gcgacattat gaatatgttc aatctccaga
     9061 gtaatgatag ccaatcaatg ggaagacggg tcatgcgaaa accgcaaatg ttaatgaatc
     9121 gaacattgcg gcaactcaat cgaaatgtgg gacggacttt ggcttttggt ggtggtgcag
     9181 cagttggaaa taagttggca aaggaacaat caaaatccaa atttaagcca tccggttctt
     9241 ctctacgaaa gaattcacga ttaccgaacg atcacgagat accatctgac ttgaataagg
     9301 aaaagccaat tccaaataat aagaaacaat cccgaatgaa tttggccggg agaaaagtcg
     9361 gcaaggttct ggatacccaa gctttggtga aagataagac caaacaagta aaggatggag
     9421 tacggaatac gccaaccaac ttgaaataca acttccataa aggaattgaa aaaacgaaaa
     9481 aagcaccgga agagtttaag cgaggactgg tccaagaaaa agcaaatcga ggagagttgc
     9541 gagaaaaaca acaacaacgt agagacgaaa aaatagctga aaagcgcaaa gtattgaacg
     9601 aaatagggaa tcctcatggg aaaagaaggg aaaatgttcc gataaaagca aaggtccaat
     9661 ctcaaaagga tagagcacca aagcggattg tgagagcaaa ttcgaatccc gaaatcaaac
     9721 gaaaattcgc tagccaagaa atcatttcaa aaggaataaa ccaatcctca agtaagaaaa
     9781 attcttttca acaagtgaaa cggcggtcta ctctgtccaa acgaaccaat cgaaaagtac
     9841 aaaaaagttt gaaccctcat cccaaaccaa ggtcaggtga gaaaaaatga acgtaaagaa
     9901 attaagtgcc atttcgttaa ctttcctaac aatctctttt atgggtatct tgatgtgtgt
     9961 gggcttattg tttggtgaag acagtgaagg aagtagtagt tcaggtgctc ctcctggggg
    10021 gagtagtgtc tcagaagagg ttttgaaaca ccgtgcaatg gtggaaaaat atgccaaaga
    10081 atcggatata acagagtatg ttccgattct tttagcgatt attcaagtgg aatctggtgg
    10141 cacaatggaa gatgtgatgc aaagttctga gtcagccggt ttaccaccga attcattaag
    10201 taccgaggca tccatcaaac aaggatgtct ctattttgcg tctttggtta agcgttccaa
    10261 agaattaggt tgtgatcaag atagtatcat tcaggcctat aactttggcg gtggttattt
    10321 ggactatgtg gcaaaaaacg ggaagaagca tagttttgct ttagctgaat ctttttcaaa
    10381 ggaaaaatct tgcggtcaaa aaatcgatta ccctaatccc atcgcgaata aggaaaatgg
    10441 tggatggcgc tataactatg gcaatatgtt ttattgctta ttggtgaagc aatacctaac
    10501 tacaacacag tttgacgata aaacagtcca agggattttt gacgaggcgt ttaagtatga
    10561 aggaacggct tatgtatttg gtgggtcaag acccgaaaca ggatttgatt gtagtggctt
    10621 gactcaatgg tgttatgcca aagttggtct gaaacttcct cgggtggctc aagatcagta
    10681 tgatgccatg acccatattg acctaaaaga tgccaaacca ggggatttgg ttttctttca
    10741 ttcgacttat gatgccggta cttatgtgac ccatgtcggt atttatgcag gggagaatcg
    10801 catgtttcat gcaggagatc cagtaggctg gacgaatcta acagaaagct attggcagca
    10861 gcatcttatc ggtgctggac ggtacaaata agagaaaaga ggaaaaagaa tgaagataaa
    10921 aattgaacgg aatcaaaagg aaaagagcct aaaaaagaaa aaagaacgag tcgtatccgt
    10981 gggtaagcat cgaaaaatgg tgcttgccct ttggctttta ttatcttgta gtcttacttt
    11041 tggcgtttat aaaaatttca ccgccattga tcaacataca actcatgaaa aagtggttat
    11101 taaagagaaa gtagtgaata cttctggtat tgaaaacttt acgaaggatt tttcgaaaga
    11161 atatttttcg tggaaaaata acaaagaagc aattgaaaag agaatgaaca acctcgaaca
    11221 gtaccttaca gaagaaggtt tcgctttaag tcaagatatg gttcgagcag atattcccac
    11281 taattccgaa gttcagtcag taaaagtctt agatgttgaa aagagctcgg aggaatttgt
    11341 cgtttcattt ttagtggagc aaaagattac agagggaaaa aaggcacaat cgatttcttc
    11401 tgcataccga gtgacgatct ttgaagatga aaacaggaat tatatagtaa cgagtttgcc
    11461 aactatgatt gcaaaacctg atagggcaaa atacaagaca aaacaagtag aaaatgattc
    11521 gaaaattgat gccaaaacta cagaagaaat cgctgagttt ttggaaacgt tctttaaatt
    11581 atatccatca gcttctgaaa aagaattgga gtattatgtt gaaaatgatg cgatgcgacc
    11641 aatcaatatt gacttaaagt tttcgaagtt agaggatcca gtttacctac aaaacggaaa
    11701 aaaaattcta gtgagtacaa agataaaata ccttgataat gtatcaaaaa ccactagcta
    11761 ctttcagtac aaattaatat tacaaaaaaa tggaaactgg aaaatcattg atgttaaata
    11821 ataatgactc aatctaaatt tatgagaagt tataagtagt ttaacttatt tcaggcagaa
    11881 gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg taactcgaaa accgacaatt
    11941 aagacatagt gcctttttat tttaatccat gtcataatca gtctatataa gaaatggttg
    12001 atataccatg gaattagata caaataatca ttcattattt cttctttgtt accaccttat
    12061 tttagtagtg aaacattgta aacatctcat tgatgatgta atgttagctt atgcaaaaaa
    12121 ctatctttga aagaatatca gaatcacatc acattacctt agttgagtga aatcgtgata
    12181 aagactactt tcatattatg ctcaaagcac aacctaaaac cgaattaaca aaattcagca
    12241 atgcctaagc tcatgtctga taaaatgaga ctttctaagg gtcaaaaatt tcctttgaat
    12301 acgatcatta gcttagataa aagtaaaaac cttagaacta cggggatagc ttggttaata
    12361 agagatatcg ctgttaataa agacataagc ttgcaagtat gctctattcc caagaatctc
    12421 ccacttcaag cactaaggat cgttaaattc agctaagtgg caagtagttc actagacgat
    12481 aattatctcc atatttcaat ctcagctttt ccatttttca tagcaaattc cgttattttt
    12541 atagcatcta aaagattcag cttttccact gatctttcac cacttttcag cttttttatt
    12601 gtgcttaaat tgataccagt accttttgat attgaattgg aagtgaaatc attaagggtt
    12661 ttttttatta aattcatatc cgcaattcca gcgttcataa gaatgaagat tctcctctca
    12721 tagactactt tgaaaatggt actacagtgg ctgtttatat atttcagtag gttactgtag
    12781 tggtcatttt attataatta gaaaaggaac ctaaatcaaa aatgctaaag aaaattttca
    12841 tagtattaaa cctatcatga ctaaagccac ggaattcttg ataaaagagt atacttgcaa
    12901 gcctatgtct ttactaacag cgatatctct tattaaccaa tctatccccg ttgttcctaa
    12961 ggttcttgat gttttctaag gcaatgcttg tatacatagc ccttgatcaa aatattgata
    13021 ctaacgttga cgtttcgatc atgatgtgta cggcaaatag gacaagtcca ttctcgaatt
    13081 gtgaatgact tcttaccatc tgtgtgtcca cattctgaac caatttgact agacggaaat
    13141 catttatcta tcttgatgat ttttttgaca gaagtatcga aaatctttac ataatctggg
    13201 tgttgcattc ttatgcagat gagagcgtta gctccgtatt cataaccagt tgcctctaaa
    13261 acgtagtcca tcggtggata cctagcttgc ggttgccagt ggctttcgca gtcttcttgt
    13321 aatcaaatcg agcatctaat tgagtactag tattttgaag ataatacttg agtttttcgg
    13381 ttttcagaag ttcacttcat cttccatcat ccatggaaat cccactcacc catacgcagg
    13441 cctccaaaaa ttcttcaaca atggtttttc agacattttt ctgaatacct ccttgttttt
    13501 tgatacgcca gatagtcagg aaaaaggcaa caaaacacaa aacaaagctg cctaaaaagg
    13561 taatcttcat tccatataaa aagacatccg gccgatctgc gatatagtta gttactcgtt
    13621 ggcccatctt agcagacatc gcttgatata agaccgtcgt agaaagcgaa atcccgatga
    13681 ccatccccaa atttcgggca aaggaattca tggagcctgc cagtcccaaa ttttcgatag
    13741 tgacagaact cataaccatc gtattatttg gggactggaa caaggcattg ccggcaccca
    13801 tgatggcagt agcgacaatg tacagccaca aaggagagtc cactgacaaa aacatataca
    13861 tgacttgggt taccgacaat aaacccaagc ctactaatac aaaaatctcc gggccaaccc
    13921 ggtcggtcag gtatccgctt aaaggcgccc cgacgaccat catcaaggga aagaccatca
    13981 tcaacagtcc ggcatagcta ggactcaacc cgcgagcatt ttgcagataa aaaggaatca
    14041 ctacattaac aaagaagttg gaagaaaaaa tcaacaaggc tgtaatcaag ctcatggtaa
    14101 aaatcttgtt cttaaaaata ccgaaagaaa tcaatggaga ggctgctttc ttttcaactt
    14161 ttaaaaagct gacaaaggca atcactgaaa ttacgaacag tacaaggggg actggatgga
    14221 aaaagccact ttcttgcccc aaaaagattg ttccaaaaag gctgataatc gtagccgcaa
    14281 tggcagcaaa gccccctcca tcgattgtat ggccgctcat ggtgatatct tttggtaaga
    14341 atttttcagc aatcaacatc gttacgattc ccaccggtac attgatccaa aaaatgtacg
    14401 cccaagaaaa ttgggacaaa atcaagccgc caagacctgg tccagcaatc gagcctaatg
    14461 aaacaagcgc accgattgct cccaatgccc gaccacgttc atgtatggga aagacttccg
    14521 tgatgattcc ggcatttgta gccatagtca tactagcacc aatggcttgt accacccgtg
    14581 cgaacaatta aatacctagc gaatgattga acccacacaa cagcgagccc aagacaaaga
    14641 tcagcgtacc aatccgaaat actttgattt tcccacagct atcgccaatt ttaccaaaca
    14701 gcaacaaaca agaacagaca atcatcagat aaacggacac tacccattcc gcttgattca
    14761 ttggtatgcc catatcatct gagatggtag gtagtgcaat attgacaata ctgccatcca
    14821 atgtggacat gaaggtaaac atagatatag caatcaaaat ccaccagcga tttttttgga
    14881 cttttgcatc ttcttgataa ctatgcataa taggatttcc tttctaatga tcgtgtagct
    14941 aagaacaata taacctatgt ctactgccca acaacttctc gcgactctag ccataggaag
    15001 cttgagtaat ttcgatttgt tattctcatg taaccatgta agtttgagat gctttatttg
    15061 aacttactct atagtaacta gtgataattc gctttacttt cttctagcaa tttctaaaag
    15121 tcgacttcat tgttcttcag attaatagtg tgagctatct cgtgagacgt aagaaaattt
    15181 ccattctact ttttcccaat tttttcatat ttatggtgtt gcacctattt gttttccaat
    15241 ttgcttttca aatacattgc tatgatattc aattccatag gattatacta aatgtcaata
    15301 gtatgaataa tatattatag atgtttatat atcactgtta attattatcc acaataatta
    15361 atgattacct cgatatttgt caatcaaagg ttgaaacaat ttattttcag catcttttct
    15421 aacttgagct gcactcttaa tagaatcata cgatcctaaa tagtacctct tttttttaaa
    15481 gttatatacg caatccattt ctctttagaa cgataaactc ccttaacacc acttgtatta
    15541 tttttcggta attttgacgt tagacactct attactgtcc cttcaacaca tgtccttgtt
    15601 tcttgccttt ttaaacatcc acaactttga gtatgtccaa aaaataagtt actataactt
    15661 atctcacaca tactgccaca ctcacattga caaacccatt ttgttttcca tgaacagtta
    15721 ttgtccaaag gatactttgc agtaagtaat ccgaattttt tattttcaat gtgatgcttt
    15781 tttgatttca aacatccaca gctttttaca tcacctttta gaagtcgctt tgccgatact
    15841 aacttttttc tcccacacag acattgacat tcccaaacaa cagttccttc acttcgcagt
    15901 ttagttggct tgattgcaac taacttacca aaagttttat ttgtcaaatt tttatattta
    15961 tgcctatttt tataatttcc acatccacaa cttttggttc tacctgatag taagtttgat
    16021 cgatatacaa tcgtttcatt cccacagtca cataggtaac gatatttttt atatccatta
    16081 tgagtaatct cctcaacaac agtaaggtga ccatatcttt ccttcactgc acctaccctc
    16141 ccaagaaaga agtaccatat aatttcaatt aacttttaat aaatcagaaa aaaatatttt
    16201 caatttttct tagtctgatt ttatgactaa tttataatac aagactgaac gatacgtaca
    16261 cgaaatcctc aagatagata aattgttttc atctagtaaa acctgctcaa aatatgagca
    16321 taaagacggc aataatacac tcgatattcg ataatggact tgttttattt gtcaaactca
    16381 tcgtaatcga gatatcaatt ccagtctcaa tatcctgatc aaaggtctaa gaatccaacc
    16441 attttcttgt tactttgtca gattgattac cttttttagt ctccgaataa taaggttttc
    16501 ggatcaacta acatgctctg agaaagacta ctatctttgc aaatttccaa atgaaggtgt
    16561 gggccagtac tattcccagt acttcctaca tagccaatta tttgtccttg caaaacatta
    16621 tctccagttt ttactaggta ttgttgctgg tgagcatata acgcggttga tccatcttcg
    16681 tgttgtatca ccacgtaatt tccccaagag tagtgaaact ctgctttaac aaccttacca
    16741 gctttaactg catgaatcgg ctcaccctgt gtagctgcca tatcaagtcc tcggtgaaat
    16801 tcatttccac gatttccaaa caaactggtt attgaataat tatgaatagg cacttggtat
    16861 ccagtcttac tcatttgtgg acctttaact tctttatcat agttggttaa atcataggtt
    16921 ttaattaatt catttagctt caaatagtat tgtgtatctg ttgcataccg tcctgtaagg
    16981 aatttagttg cttctcgata tgttttacta ttagctttta atgctcctgc ataaaaatct
    17041 ttatttccac taattccctt agttaataat ttggcataat cttcaaaact ttcctcatag
    17101 ttttcataca ctctaaattt tgattgggtc gtataaagcg ttccattacc caaatcttcg
    17161 tttgttacca taatgacaaa gtttccatta taagttcctt ttatcccaaa taaattgtaa
    17221 ttcggtgcct gagctaactg gctttgacca ctggcagatt ctaagatcgc ttgtgcaatc
    17281 ataacagaag catataaatc atatttctga ccaatttttc tcgcagactc accaattttc
    17341 ataataaaat tttctacaga ttcatttttg tcaaaatgaa tttcttcatt accagatagt
    17401 attggttgtg attggaaaga ttctgttgat tctatttgct gtggtgtcga aaaatttttt
    17461 gtttgtcctt ccgtttttgg cgcttccttt tttgaagact ctatatggtt tggtcttgtc
    17521 actttcgaag aagttatttc acttgtacta cttgaagttg ttgaagattc ggcacttgaa
    17581 atcgtactac tttcacttga cgaagttgtt gaagactcgg catttgaagt cgtactactt
    17641 tcacttgacg aagttgttga agactcggca tttgaagtcg tactactttc acttgatgaa
    17701 gttgttgaag actcagcatt tgaagtcgta ctactttcac ttgataaagt tgtcaaaagt
    17761 tcactactct ctgctgttac atcggaatta ctcatttcag tcgtctcggt tctttctttt
    17821 gcaattttgt atgttgaatt ttcgcttgcc aacacatgga aaggtgttgc tataaagagt
    17881 tgggacatta atataactgc tgatgcaagt tttaaagatt ttttcatttg atttacctct
    17941 cttttttctc tttaatagtg ttttctatta aaaattatga taaatatttt caatgaattg
    18001 agtaatacag tatatttctt gatttcattt agtaacgatt atgtttcaat gtttaatcct
    18061 gttactgcct ttaaaataaa attatagggt tggctaaaac taggatgaaa taaataatct
    18121 aacattttca atttgtctaa tgtaatttgt tcttctatag caagagaaaa cattgataat
    18181 agtccagata aatcttcttg actacaaagt tgtacaccta ctattctctt tgtatttttt
    18241 tcaaagctaa ttcttaattt tacacgacta ttttttctca aaaaaaca
//
LOCUS       DAGCWU010000045        17715 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.045, whole genome shotgun sequence.
ACCESSION   DAGCWU010000045 DAGCWU010000000
VERSION     DAGCWU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.045"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            4..816
                     /locus_tag="MBZ71_001216"
     CDS             4..816
                     /locus_tag="MBZ71_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBT4429180.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="MBZ71_001217"
     CDS             813..1613
                     /locus_tag="MBZ71_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBT4429181.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="MBZ71_001218"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="MBZ71_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBT4429182.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="MBZ71_001219"
     CDS             3110..4819
                     /locus_tag="MBZ71_001219"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBT4429183.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="MBZ71_001220"
     CDS             5031..9383
                     /locus_tag="MBZ71_001220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBT4429184.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="MBZ71_001221"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="MBZ71_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBT4429185.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="MBZ71_001222"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="MBZ71_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBT4429186.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="MBZ71_001223"
     CDS             11221..11514
                     /locus_tag="MBZ71_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBT4429187.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="MBZ71_001224"
     CDS             11511..11822
                     /locus_tag="MBZ71_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBT4429188.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="MBZ71_001225"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="MBZ71_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBT4429189.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="MBZ71_001226"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="MBZ71_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBT4429190.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="MBZ71_001227"
     CDS             complement(14623..14844)
                     /locus_tag="MBZ71_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4429191.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="MBZ71_001228"
     CDS             15292..17655
                     /locus_tag="MBZ71_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HBT4429192.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWELFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6036 a         3146 c         3903 g         4630 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaatccaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcttttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaaagat tatccttcgc tgaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgtatt
    17701 atttgtcaaa ttaag
//
LOCUS       DAGCWU010000046        17538 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.046, whole genome shotgun sequence.
ACCESSION   DAGCWU010000046 DAGCWU010000000
VERSION     DAGCWU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.046"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(41..850)
                     /locus_tag="MBZ71_001229"
     CDS             complement(41..850)
                     /locus_tag="MBZ71_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4429193.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1143..2579
                     /locus_tag="MBZ71_001230"
     CDS             1143..2579
                     /locus_tag="MBZ71_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBT4429194.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNEVSVALWIVDILFIINFIILATI"
     gene            2749..3945
                     /locus_tag="MBZ71_001231"
     CDS             2749..3945
                     /locus_tag="MBZ71_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBT4429195.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSESTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFILSNIFLSLFNFRK
                     VLRSRL"
     gene            complement(4007..4192)
                     /locus_tag="MBZ71_001232"
     CDS             complement(4007..4192)
                     /locus_tag="MBZ71_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBT4429196.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4419..7733
                     /gene="dnaE"
                     /locus_tag="MBZ71_001233"
     CDS             4419..7733
                     /gene="dnaE"
                     /locus_tag="MBZ71_001233"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBT4429197.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLIKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLIENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKDGSQIPLENLSASLPLT
                     ANGYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITNYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLGPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            7901..8863
                     /gene="pfkA"
                     /locus_tag="MBZ71_001234"
     CDS             7901..8863
                     /gene="pfkA"
                     /locus_tag="MBZ71_001234"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBT4429198.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8937..10721
                     /gene="pyk"
                     /locus_tag="MBZ71_001235"
     CDS             8937..10721
                     /gene="pyk"
                     /locus_tag="MBZ71_001235"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBT4429199.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10756..11658)
                     /locus_tag="MBZ71_001236"
     CDS             complement(10756..11658)
                     /locus_tag="MBZ71_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4429200.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11844..12413
                     /locus_tag="MBZ71_001237"
     CDS             11844..12413
                     /locus_tag="MBZ71_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBT4429201.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12471..12650
                     /gene="rpmF"
                     /locus_tag="MBZ71_001238"
     CDS             12471..12650
                     /gene="rpmF"
                     /locus_tag="MBZ71_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBT4429202.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12820..14241
                     /gene="gndA"
                     /locus_tag="MBZ71_001239"
     CDS             12820..14241
                     /gene="gndA"
                     /locus_tag="MBZ71_001239"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBT4429203.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14434..15120
                     /locus_tag="MBZ71_001240"
     CDS             14434..15120
                     /locus_tag="MBZ71_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4429204.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15117..16622
                     /locus_tag="MBZ71_001241"
     CDS             15117..16622
                     /locus_tag="MBZ71_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4429205.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            17309..>17538
                     /locus_tag="MBZ71_001242"
     rRNA            17309..>17538
                     /locus_tag="MBZ71_001242"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         5675 a         3055 c         3679 g         5129 t
ORIGIN      
        1 aaattgtcac aacctctttt ttgtggaatc taattctcta ttatagttta agtagttttt
       61 ccagataaac agctaaaccg tcttcctgat tgctcaaagg agtgacatca tttgcaatgc
      121 ctttgagttg gtcagtaccg ttagccatag ctactcccca acctgcataa tccaacaatt
      181 ccaaatcatt atgctcgtca ccaaaggcaa tgacgtcttt ttgatcgata gacaggtagt
      241 tagcaatctc ttgcacgcct tttgcttttt ggattccttt tgaaacaatc tctaagatgg
      301 cagtaggtcc accccatgtc cgaacatcga cataactgcc aaattgacgt gttaattcag
      361 cagatacagc tcctgcaaaa cgtttgtctg ttcggacaag caaagatgta ggatttgtcg
      421 tcaaattttt aggagaaagc aaattcttct ctcctggacg agaagaagca aaaatttttt
      481 catcaaagaa atcaaaactg tcaataaaaa aagtatcacg attttcagca gcgataaaat
      541 ctaaatttag ctgctttttt tgcgataaga tttcaaaagc gatttcacga ttgatcaacg
      601 tttctctttc accatcccag tgctgattag ggatgtgtac taatgcgccg ttaaaattaa
      661 ccatcggtgt tgttaattct aattgttggt aaaactgatg gctcattcgg aatgggcgtc
      721 ctgtagcaat actgacatga tgaccggcat tgattgcttt ttttagagtc tcttttgttt
      781 tagatgtaat catcgaatct tgattgaggg tagttccgtc cagatcgatg gcaattaatt
      841 ttttattcaa taagggtcct tccttcctca actagcattt gttttagtct agcatatttt
      901 aggtaattaa caagagcaat cagctattcc taacaaaaac tacagttatg gctatataaa
      961 tagctaaaca taagctaaac acgaacgtta agtattattt tcgctttttt tgttaaaaaa
     1021 acgttgaaaa gcgaatgaaa cgtctatata atttgaaatt gttcgatgga tttgccctat
     1081 tttacttatt ttttattatt atagttttcc atcagaaaaa aattcaggga gtggaacttt
     1141 ttatggaaaa gtttttcggt ttaaagaaaa acaatacaaa tgtatcaact gaaataatgg
     1201 cgggagtaac gacattcttt gccatgagtt atatcttatt cgtaaatccg acgattttgt
     1261 cagcatcagg tatgcccttc caagcagtat ttctagcgac aatcattgca tcgattattg
     1321 gtacgctggt aatgggattg ttcgcaaatg taccatatgc tcaggcacct ggtatgggat
     1381 tgaacgcatt tttcactttt acggttgttt ttgggcttgg ttattcttgg caacaagcct
     1441 tggcaatggt gtttatttgc ggattaatca atatttttat tactgtcaca aatattcgaa
     1501 aaatgattat tcgggcgatt cccgaaagtc ttcaacatgc gattggtggc gggatcggta
     1561 ttttcgttgc gtatgtcgga atcaaaaatg caggattctt atctttttca gcagaccagt
     1621 cagctatttc gagctctgtc gttgaaggcg gaaaagcgac aaatgttacg ataaatggtg
     1681 gaatcgtccc tgcgttagcc aactttgaca acgctcctat cttattggct gtgattggtt
     1741 tggtactgac agccatttta gttgtaaaaa atgtccgtgg ggctattctt atcggtatcg
     1801 tagcgaccac agtccttggt atttttatgg gtgttgttga tttaagttcc attgattggc
     1861 aaacgaattc gttaggtaat tcatttaaag aattaggaac aactttcggt gcagcatttg
     1921 gttctgaagg catgcaatct ttattcagtg attcttccaa gattcctcaa gtattgatga
     1981 cgatcatcgc attcagttta tctgatactt tcgatacgat cggaacattt atcggtacag
     2041 gacgccgcac aggcattttc tcaaaagaag acgagatcgc gttggaagat ggtcgtgggt
     2101 tcaaaacaaa aatggataaa gcactatttg ccgatgcgat cgctacttcc gtgggggcta
     2161 tctttggtac atcgaataca acaacttatg tcgaatctgc agctgggatc ggggccggtg
     2221 gacgtactgg tttgacttca gtagtagtgg cagtgctatt cgcattaagt agtttattct
     2281 ctccactgat cgccattgta ccaaatcaag caactgcgcc agcattgatt ttagttggag
     2341 taatgatgat ggcttctttt gcagacatca aatggctaga tatggaagaa gctttaccgg
     2401 ccttctttgc atcgatcttt atgggattgt gctatagcat ttcctatggg attgcagcag
     2461 gatttatttt ctatacaatt gtgaaagtaa tcaaaggaaa atacaatgaa gtttctgttg
     2521 ctttgtggat cgttgatatt ttattcatta tcaacttcat cattctagca acaatttaac
     2581 taaagagaag ggattggaac agtatttgtt ctgatctctt tttgttttac ataaaaagaa
     2641 cgttacatat aaaatgacct ttgcgggatt tttctctttt tactggcgag atcgttgctt
     2701 gtttattcga aaataggtat gataaaagag acgaaagagg gatgatagat ggagattaat
     2761 tggagaaaaa atttagtgat tgcgtggatt ggctgttttt tcactggtgc aagtattagt
     2821 ttagttatgc cattcatacc agtttatgtt gagcaactag gaacaccaaa agatcaaatc
     2881 gaattgtttt cgggactagc aatctctgtg actgcttttg catctgccgt ggttgcgcca
     2941 atctggggga atctagctga tcgaaaagga cgaaaactca tgatgatccg tgcagcagca
     3001 gggatgacat taacaatggg atctttggct tttgtaccga acgcgtattg gcttttgatc
     3061 atgcggtttt ggaatggaat tttgtctgga tatattccaa atgccacagc gatgattgct
     3121 tcacaggcac ctagagaaaa gagcggatgg gcgttaggaa cattgtctac cggagcgatt
     3181 gctggaaatc tgatcggacc ttcaatgggc ggtgcactgg ctcagtggtt tggtatggaa
     3241 aatgtattct tgatcacagg ggctttgttg atgatcacaa cggtattgac cattttctta
     3301 gtcaaagaag atttccatcc gatcgagaaa aaagatctga taagcaccaa agaaattttt
     3361 tcaaaaatgg atcatctctc tattttgatc ggtctcttca ttacgacctt gattttacag
     3421 ataggaatca ctacgatcag ccctattttg accttatata ttcgtgaatt gagcgaaagt
     3481 acagaaaata tcctttttgt cagcggcttg attgtttcga tagctggtgt ttccgctgtt
     3541 ttttcttcac cgaaactagg aaaactaggg gataagattg ggaatcaaaa agttctaatt
     3601 ggaggactca ttttatcatt ttgctgttat ttacctatgg cattcgtcac tacaccgctc
     3661 caattaggta tacttcgctt tattttaggc ttttcaactg gcgctttgat gccttctgtc
     3721 aatacactga tcagtaagat tacgcctcaa gaaggagtca gccgaatcta tagctacaat
     3781 cagatgttta gcaactttgg acaagtttta ggtcccatgc taggatcgac agtcgcgcat
     3841 gcttataatt acagtgctgt ttttattgtg acaagtttgt ttattctgtc aaatattttt
     3901 ctttctttat tcaactttcg taaagttctt cgcagcagat tataatggtg gtgaggagca
     3961 gaaaataaaa aagagcttga gatatctcaa gctcttggct gtttttttac gaatgggcag
     4021 aggtttcttt tcttggcgca actgcaccaa tcaaaaggat aatgacaccc gcaatgaatg
     4081 aaatgattgt tgtcaaagta aaatcatatg ttccgcttgt taatgcagca ccaatataac
     4141 cgactacttg acctagaacg acagcccaga ataatgtaac gatatatcgc ataaagaaca
     4201 cctcttttct atcttaattt taccagattt tctttgaatg taaagaaacg agaccaaatc
     4261 tcaataaaat ttgagttctt gcgaaagaac tcattcctac agattacaag caaatgaagt
     4321 ggaataatca acttcagcgc ctgctatctt aatgaaagta aaaggatcaa accttttaac
     4381 gctagtgaat tttaacgaaa aaaaaggcgg aatgcaagat gtttttccca caactttata
     4441 caattacttc ttattctctt ttacaaagta cgatacgtat tcgagaatat gtacaagaag
     4501 caaaaaaaca aggctacact tcattagcca taacggataa agatgtctta tacggtgcac
     4561 ctgaattcta tcgtatttgt gtagaagaag gagtacagcc aatcattgga ttacatctta
     4621 gttatactta tgaagaaaaa aattatggct tgcttgcgta cgcaaaagat gaaataggtt
     4681 atcagcagct gataaaactt tccagccgaa aaatgatcca agaaaaattg gaacttgcag
     4741 aaatgacaga aaccgaccat ctagcgtttg ttgttccggc aaatcattct gtttctgagg
     4801 ctttcaataa agaggataac tttttcggtc attggcagtt tttacttgaa aatgttttta
     4861 ctagaaattt ttatatcggc ttgatagaaa acacgcctga tcaattgcct gaatggtatc
     4921 agtatgtgat aaaagaaaat tattcgcttt gtgcattaca tcccatcgct agtttacaac
     4981 aagaagaact ttttgcactt gaagtgatga aacatatcaa agatggaagc caaatacctt
     5041 tagaaaattt atctgcatct ttaccactaa cagcaaatgg ctatcttctt tcagaacaag
     5101 aaatgatcaa acgattcgaa aaagcgggtc tttcccaagc tgtaataggc gcccagcgtt
     5161 tagctgaatc gatttcattt gaattccagt tccatcagaa attacttccg cattatccag
     5221 ttcctaatgg aaaacaagct ggagaatatt taagagagtt atgctattcg caagtggaac
     5281 gccgaatcac taattataca aaggaatacc aagaacgttt ggactacgag ttatctgtca
     5341 tccatcaaat gggattcgat gattatttct tgatcgtatg ggatgtaatg gcttttgctc
     5401 atagaaacaa aatcgttacc ggtgcaggtc gtggttctgc tgcgggttca ttagtagcct
     5461 atgtgttaga aatcacagat gtggatccat tagaatatga tttgctgttc gaacgctttt
     5521 taaaccccga acgctttacg atgccagata tcgatttgga tattcctgac aatcgacgtg
     5581 aagaagtatt gaattatgtt cgagaaaagt atggacaata tcatatggca caaatcgcaa
     5641 catttggcac aatggcagca aaaatggttc ttagagatgc agctagagtg tttggtttat
     5701 cgcaaagtga agcaaaccgc tggtcaaaag caatacccaa tcaattgaaa atcacattga
     5761 cccaagctta taaaacttcg catcaattag tagaattagt tcaggcaagc gaaaaaaacc
     5821 aacttttatt caaaactgca gtgattttag aaggattgcc aagacatgtt tcgactcacg
     5881 cagcaggtgt agttatcagc gataagaatc tacttgaact agttccgctc caacgcggtt
     5941 ctgatgaagc ttttctgacc cagtatacga tgaatgaggt agaagcaatc ggtcttttga
     6001 aaatggactt tttaggacta cgcaacttat cgattattga ttttactttg aaagctgtta
     6061 agcgagtaga agggtatgag atccgattaa aagagatccc gctaaatgat gcaaaaacgc
     6121 tgctcttgtt tcaacgaggc gaaacctcag gagtatttca atttgaatct gctggtatcc
     6181 ggaacgtttt acgaagatta ggtccagaaa acatcgaaga tattgccgca gtcaatgcat
     6241 tgtatcgacc aggccctatg caaaatatag ataccttcat tgccaggaag aaaggaaaag
     6301 aagccattcg ctacccagat gattcactga tcccgatttt gaaaaacaca tatggagtga
     6361 tcgtctatca ggaacaaatc atgcagatcg cctcgactat ggctggattt acattagggc
     6421 agtcagatat tctgcgacgg gcagtgagta aaaagaaaaa agacgtatta gatgaagaaa
     6481 agtcgcattt tgtatcagga gcattacaac agggatattc ggaaaaaaca gcagcgaccg
     6541 tatatgaata tatcgaaaaa tttgcaaact atggattcaa tcggtcacat gcatttgctt
     6601 attcattcgt gggattccaa atggcgtatc taaaagccca tcatccaggt gctttcttta
     6661 ccagcctgat gaattctgtt cggcataaca catcaaaatt gaaagaatat attgcagaag
     6721 cacgtaaaaa taaactcaaa ctttcctcgc cgtccattaa tcaaagtaat tccagttttg
     6781 agttggtcaa taaggaagaa atcagattcg gactgacagc gataaaaggt attcggcgtg
     6841 attttgtaga agatatatta aacgaaagaa aacaaaatgg cccgttccag tcagtggatc
     6901 aattcctcat acggatcgat aagcgttggc tgaaattaga gttactgcaa tcactgacag
     6961 cagtaggcgt ttttgatgaa ttatctccta acaggaaaca attgatgctt gatctcgaag
     7021 gaaaaatcca aaatatcgtt tacagtggtg gaagtcttga cttgctggga atcatggcat
     7081 taaaagaaga agaagtcgct gactatacac tagaagaacg cttgaagctg gaagaggagt
     7141 acttaggtat ttatttgtca ggtcatcctg cagaagggtt tgatcgtctt cgtttagcta
     7201 aacaagttca gctgattaca gatatcgtcc ctaatcaacc agtcaattta ttagtgatga
     7261 tcaaggaagt tagagaaatt cggacaaaaa aaggagaact gatggccttt gttgaaggaa
     7321 ccgacagtag tggagaaatt tctgtcacag tttttccaac tctttatcgt aaatatcgta
     7381 atttgtttgc cgttgatcga gtgatctttg ttcaagggcg gagcgaaatc agcaaatata
     7441 atcaggaact gcaactaatt ggagaaagct ttgctgatcc taaccagtta gaagcccagt
     7501 atccttcaca aacttgctat ttgcgtatta cagaagaacg ggaagatccc gagataatga
     7561 aacaattaca agtgattttt cagaaacacc caggatatat accagtagtt atgtatcacg
     7621 aaaaaacgca acgaaaagta gttctgtcag atgcttattg ggtggatgac agcaagagta
     7681 taaaggggca acttatttat ctgcttggcg aagataatgt ggttttcaaa taacttacgg
     7741 taaaaaatac tacttgcttt catttttttt cattttttta tccgtaaatc aagacaagtg
     7801 agaaaaataa tgttagaatg ttagtcgtgg ttaacgcaaa aatggttgta aacctaagtc
     7861 atacttagga taaagtattt taatgaggtg aatgtttatt atgaaacgca tcggaatttt
     7921 gaccagtggc ggagacgcac ctggaatgaa tgcagctgtt cgtgccgttg ttcgtaaagg
     7981 tatttacgaa ggactggaag tatacgggat caattatggt tttgccggtt tagttgctgg
     8041 agatattcgt cgtcttgatg tggcagatgt aggtgacaag atccaacgtg gtggtacatt
     8101 cttgtattct gcacgttatc cagaatttgc aactgaagaa ggtcaattaa aaggaatcga
     8161 acaattgaaa aaattcggaa tcgaaggtct agtggttatc ggaggagacg gctcttatca
     8221 tggcgcaatg gctttgacaa aacgcggttt cccagcagta ggtattcctg gaacaatcga
     8281 taatgatatt cctgggactg atttcacaat cggttttgat acagcaatca atacagtact
     8341 agaatcaatt gaccgtattc gtgacacagc gacttctcat gttcgtacat tcgttatcga
     8401 agtaatggga cgcaatgcag gagacatcgc tttatggtca ggtgtggctg gcggagcgga
     8461 tgaaatcatc atcccagaac atgattttga tatggcttca gttgctaaaa aaatccaaga
     8521 aggccgcgat cgcgggaaga aacattgctt gatcatcttg gctgaaggtg taatgggcgg
     8581 aaatgaattc gctgagaaac tttcagaatt tggcgactat catacacgtg tatctatctt
     8641 gggacacgtt gtccgtggtg gagcaccaag cgcacgcgac cgcgtgttgg caagtaaatt
     8701 cggtgcttac gctgtcgaat tgcttcaaga aggaaaaggt ggattatgtg tcggtatcca
     8761 tgacaacgaa atcgttgcgt ctgacatcat tgatacactt gaaaacaaca aacataaacc
     8821 agacttatca ctatatgatc taaataattc actttcattt taagagtgaa agaaaccaag
     8881 ctatttaagc tgtgtacagc tttaaataaa caaaattgaa tgggagagtt ttttaaatga
     8941 aaaaaaccaa aatcgttagt acattaggac cagcaagtaa ctcagtagaa attatttctc
     9001 aattgattga atctggggca aatgtcttcc gctttaactt ttctcatggt gaccatgaag
     9061 aacaattatc acgtatgact atggtccgtg aagcagttaa aaagacagga aaagatgtcg
     9121 gtattctttt agatacaaaa ggagctgaaa tccgtacaac tgttcaaggc acaacagaag
     9181 ctgattttgg tcgtgcagga tacatcaaat ttgaagtagg agatcaaaca cgcatctcta
     9241 tggatcctga acatgtaggt tcaaaagaaa aaatcgcggt tacttaccca ggattatttg
     9301 acgatgttca cgttggcgga cacattttat ttgatgatgg tttgatcgat atgcaaatca
     9361 ttgaaaaaga cgaagcaaac cgtgaattag tcgtagaagt taaaaatgca ggtatgctag
     9421 gatcacgtaa aggggtaaat gcaccaggcg tttctattag tttgccaggt attactccaa
     9481 aagatgctga cgatattcgt tttggtttag acaacgatat cgactttatc gctgcaagtt
     9541 ttgtacgtaa agcacaagac gttctagata tccgcgaaat cttagaagaa aaagacatga
     9601 ctcatgttca aatcttccct aaaatcgaat ctcaagaagg tatcgacaac attgacgaaa
     9661 tcatcaaagt ttctgacggt gttatgattg cccgcggaga tatgggtgtt gaaatcccag
     9721 ctgaactagt accaatggtt caaaaacgca tcatcaaaaa atgtaatgct gctggtatcc
     9781 cagttatcac tgctacacaa atgttagaat caatgcaaga aaacccacgt ccaacacgtg
     9841 cggaagcttc tgacgttgct aacgctgtat ttgatggcac agatgcaaca atgctatctg
     9901 gtgaatctgc aaacggggac tacccagttg aagctgtttc aacaatggca cgtatcgacc
     9961 aagaagctga aaatgcatta tcagaattag gtacattcca attgaacgaa ttcgataaaa
    10021 cagatgtaac tgaaacaatc ggtttatctg tagctcgtgc tgcaaaaaat ctaggcgtga
    10081 aaacaatcgt tgctgcaact gaatcaggat acactgcaaa aatgatttca aaatatcgtc
    10141 cagatgcaga tatcttggct gttacttttg acgaacgcac aaaacgtggt ttgatgttaa
    10201 actggggcgt ttacccaaca gttgctgaaa aaccatcaac aactgatgaa atgtttgaac
    10261 tagcagctaa aaaagctgtt gaattaggtt tcgcatcaga aggcgattta atcttgatca
    10321 ctgctggtgt accagttggt gaacgcggaa caactaacgt gatgaaaatt caaatgattg
    10381 gttctaaatt attagaagct caaggcgttg gacaaaaatc tgtcgttgca aatgcagtta
    10441 ttgcttcatc tgctgaagaa gctgtaaaaa aagcaaaaga cggaatggtt ctagttgtac
    10501 catcaactga caaagaatat atgcctgcaa tcgaaaaagc agctgctgtt atcgttgaag
    10561 aaggcggatt gacttctcac gctgcagttg taggaattgc acaagatatt cctgtaatcg
    10621 ttggtgctaa aggcgcaaca acaacaatta cagaaggtga attgattacc gttgattcac
    10681 gtcgcggcat cgtttaccgt ggcgaaacaa cagctatcta atttagctta ataaaaaata
    10741 aaaacagctc ttcttttacg aagagctgtt tttttgtgct gtaacgaatt catggataac
    10801 ttggactact tgttcttgaa gaggcgtgac gatctgctgt gttcgttttg ccaatgaaat
    10861 caagaagcga ggctgaactt catctgaaag agggaggcaa actaaatgat cttcaggatg
    10921 gacagcaatc tctgccaaaa aaccgatacc cacattttct cggaccatac tctttaaaat
    10981 cgtcaagtca ttgctttgat aaacaacttt tggatgagta tgtgtttgtt tagaaagctt
    11041 agtgaatgct gcggggtgga cataatgttc gttcaatagt acaaactgtt catctgctat
    11101 atctttgaaa gagacagtat cctgcttgga aagaggatgt gtaggtggca cgacgatcat
    11161 gaaacgtttg tccaaaagta actctgtttc taaatattga tccacgattg gctgtgttga
    11221 gcctaataca gctaaatcga tcctcccttg tcggatcatc ccgtataagt cccgagagcc
    11281 tccatcggta atctcaattt gattcatcaa ttcatgcttg ataaagtaac ttgacagttt
    11341 aggaaagtat tcattgccga taataggagg catgccaaaa cgaagctttt tcttttgcaa
    11401 ctgagtgatt tcagctcttg cagtagaaat ctcttggaga atggtttgtg catggagaaa
    11461 cagctgcttt ccagaaggcg tgacgattaa ttcgtgatgc gatcgatctc ttgtaataag
    11521 tacggtgtca aactcttttt caagacgttg taatgcatat gtaatagtag gttggctcac
    11581 acggaaataa ttagccactt tcgtaaaatt tttttgtttg acgatttgga catagtattc
    11641 gatatctttc gtgttcatga agatcatccg ctcctttaat ggaataatga ttattgtaaa
    11701 tgattctgag gatgatagca aacagaggct ttctaataaa gatttttctg tttttttatg
    11761 aactgcaagg tgtcaagaaa atttcattga caacatgctt tttgccctgt ataattgctt
    11821 ttgttgctta ggagtgatga aaaatgaaat ggtctttatt ggaattgaga aaataccagg
    11881 agacaccgtt aacttttcat gagacgctcg acttgaaagc cgctctcatg aagcgagaca
    11941 atttaatact tgatgttgcg ccagtggaag tcgaaggttt ggtctcagtt gataagaaag
    12001 gctatatcgt tcattacact gtgcagacga ctttgacagt tccttcaaca cgttcactag
    12061 aacctgttga actgcccatg gatttttctg tagacgaagt attcatgaca gaagaacaat
    12121 tccaaagtag agatgaacaa atgtctgcgg atgagatttt gctaattgaa ggtccattat
    12181 tgaatttagc agagtcgatt gaagacaaca tcttattggc gattccaatg cgggtattga
    12241 caaaagaaga ggaagcagca acagaacttc caaaaggcaa tgattgggaa gttgtttccg
    12301 aagaagaata ccagcgcaga aaaatggaag aagccgagaa taaaattgat cctcgtcttg
    12361 caaaactatc tgaacttttt gattctaaat caaaagacga cgataacaag taagattgga
    12421 atatattgtg tctaatttag atacaagact ttacaaggag gtgtagcaaa atggcagtac
    12481 cagctagaag aacatcaaaa gctaaaaaag caaaacgtcg tacacactac aaattgacaa
    12541 tcaaagggtt aaacgaatgt ccaaactgtg gtgaaatgaa aaaaagccat cacgtatgtg
    12601 caaattgtgg ttactatgat ggtaaggacg taatgtcaaa agaagcttaa tacattttaa
    12661 ttagaaaatg taaaacctca aggctaacca tggagccttg aggtttattt ttttgtagtc
    12721 ggtaaataaa aaatatgttg ttctttagtt tgcaatgtag ccgtgttaag ctataatgta
    12781 agagattagg gagaaatccc gtatgtagga ggagttcata tgtcaaaaca acaaatcggc
    12841 gttgtcggta tggccgttat gggtaaaaat ctagccttga acatagaaag ccgcggctat
    12901 tcagtggctt tattcaatcg tacaggggct aaaacaaccg ctgtagtaga agaacaccca
    12961 gataaaaatt tcaaagcaac atatacaatc gaagaatttg tagaatctat cgagaaacca
    13021 cgtcggatct tgctaatggt caaagcagga ccggcaacag atgcaacgat ccaagagttg
    13081 cttcctcacc ttgataaagg cgatattttg attgacggag gaaatacatt ctttaaagat
    13141 acgatgcgtc gtaatgaaga gttagccaac tctggcatca actttatcgg tacaggcgtt
    13201 tccggtggtg aagaaggagc tttaaaagga ccttcaatca tgcctggtgg acaaaaagaa
    13261 gcctatgcgc tagttgcacc aatcttagag caaatttctg caaaagcaga agacgaagca
    13321 ccatgtgtca catatattgg cccaaatgga gccggacatt atgtgaaaat ggtacacaac
    13381 ggtattgagt atggagatat gcaactgatt gctgaatctt atgacttaat gaagaatatt
    13441 ttaaaccttt cagtagaaga aatggctgat atctttaaag aatggaatca aggcgaatta
    13501 gacagctatt tgatagaaat cactgctgac attctgacac gtaaagacga tgaaggaaca
    13561 ggtaaaccag tagtagatgt gatccttgat gcagctggga ataaaggaac aggtaaatgg
    13621 acaagccaaa gcgcgcttga cttaggtgtg ccacttccat tgatcactga atctgtattt
    13681 gcccgctata tttctgctta taaagaagaa cgcgtacaag caagcaagat tttatctcgt
    13741 acgaacgatt ttgagttcac gggagacaaa aaagaattgg tcgaaaaaat ccgagaagcg
    13801 ttgtatttca gtaaaatcat gagttatgcc caaggatttg ctcaattacg tgtcgcttca
    13861 aaagaatttg attgggactt gccatttggt gaaatcgcaa aaatctggcg cgctggatgt
    13921 atcatccgag ctcgattctt gcaaaaaatt acggatgcct atgacaaaaa tcctgagatc
    13981 gaaaatttat tgctagatga ttacttcgtt gaaatcacga aaaaatatca gcaatcagtc
    14041 agagacgtcg ttgccttggc tgtccaagct ggcgtacctg taccgacttt ctcatcagcc
    14101 atcgcttact tcgattcata cagagcagaa cgtctgcctg caaatataat ccaagcccaa
    14161 cgcgattact ttggcgcaca tacctatgaa cgtgtcgaca aagaaggaat cttccattac
    14221 tcatggtacc atgaagaata atagtctttc ggctgttagg aaatgagaaa ataaaaagag
    14281 gacgaaagag ctgaagtcga ttgttatgag tagacttcag ctctttttta aaattgctat
    14341 ccatgataaa atgactatag atttttaagc atattatcgg tataatgata gagtttttaa
    14401 aaagccagta gaatagaaag gataaaataa taaatgagca atattctaat cattgaagat
    14461 gaaaagaact tggcacgatt tgtcgagcta gagttgaagc atgaagggta taacgcagaa
    14521 gtccactaca acggccgaac tggtttagat gcggcattaa ataatgagtg ggatgccata
    14581 ttgttggatc tgatgctccc agaattgaac ggtttagaag tctgcagacg tgtacgccaa
    14641 gtcaaaaata caccaatcat tatgatgact gcaagagatt ctgtgatcga tcgtgtatct
    14701 ggacttgacc acggagcaga tgactacatc gtcaaaccat ttgctatcga agaattatta
    14761 gcacgtcttc gtgcattgtt gcgtcgaatc gatattgaag gcgataaaaa tgtggctaaa
    14821 caaactacta ttacttatcg cgatttgact atcgaaaaag aaaaccgtgt cgtacgtcgc
    14881 ggaaatgaag taattgaact aacaaaaaga gaatacgagt tgctgcttac tttaatggaa
    14941 aacgtgaacg tggtattagc acgtgacgtt ttactaaata aagtgtgggg atacgaaaca
    15001 gaagttgaaa caaacgtagt ggacgtatat attcgttatc ttcgcaacaa aattgacgtt
    15061 cccggggagg aaagctatat ccaaactgta cgcggaacag gttatgtgat gcgctcgtga
    15121 taaagccgaa aaaatcggaa aaaagagaac tgaaagggcc ctccttgaca atcaagtggg
    15181 cctttgcgag ttctttcttc atattcgttg tgtttacaat tttcgctgtc attacctata
    15241 aatcatctat caatctgatt gtggcaaaag aaagaaacaa tgtagagcgt acgatttcag
    15301 aagtagcttc tcgtttggct aactcggatg aagaactgac attagtaaat acgtatcgta
    15361 atctgacgaa agcgacaaat gacggagatg aggtcgtaga tcatacttca ctggtcgaag
    15421 gaaacttaat gaagatcgat tcctttatag ctgagctagg gcagccatca atggatcttt
    15481 atgtttatga tttggacgag aacttgattt ttaaaaccca tgaaaacagt cgaaacctga
    15541 ttcagactac acgtaaagtt ccaacgatcg ttacactaga aggaaaaact ggctttttat
    15601 ctatccagcc tatatattct aaaggtacaa gagaaaagat cggctatgcg caaagcttct
    15661 atgaattatc ttctttctat gatattcgaa ataagctatt attgactctt attgttttag
    15721 aagttgtttc tttgatctta agtagtattt taggtttctt tttatcttct tatttcttaa
    15781 aaccactcaa ggtactcaga gatacaatgg atactatacg taaagatccg caatcagata
    15841 tccacatgcc tgaaatcgac acaaatgatg aattggcaga tttagctgaa atattcaatg
    15901 agatgcttga ccgtatgcgt ttgtatattg aacaacaaga gcaattcgtg gaagacgtat
    15961 ctcatgagtt aaggacacct gttgcaatta ttgaagggca tcttagtctg ctaaatcgtt
    16021 gggggaaaga tgatcctgag atattagaag aatcattaag cgccagcatg caagaaatta
    16081 gccgtatgaa aagcttagtt caagagatgc tagacttatc aagagcagaa caagtagatg
    16141 ttcattatgc gaatgaaaca actaaaggaa aagaagtcac ctatcaagtt tataataatt
    16201 tcaagatatt atatccagac tatgtgatca ctttagatga tgatttgaca aaagaagtga
    16261 ccctgcaaat ttatcgtaat cactttgaac aattaatcat tatcatattg gataacgcag
    16321 taaagtattc aactacacga aaagaagtcc atatctctat ttcttccact ctgaatgaat
    16381 ttgagatcgc gattcaggac tttggcgaag gaatacccaa agaagacctt aataagatct
    16441 tcaatcgttt ttatcgagtg gataaagcaa gagctagaac gaagggtgga aatggtctgg
    16501 ggttgtctat cgctaaacaa ctggttgaaa actataaagg acgtatctta gcagaaagtg
    16561 tagtagggca agggacaatt tttagaatct ttattcctat cgcaaaggca gaagacaagt
    16621 aaagcatgtg ctttacttgt ttttttatat atccaacaaa gaataaggta caaaatacaa
    16681 gcaagttaga aattcaggga tgtcattata aaaaaaacta gcgaacaatt aaatagaata
    16741 acatccgtta attaaacgta agtagctttt agatgaacga aaaaacaatg aaatgctgta
    16801 atattcgtgt ttaaaaataa taaataaaaa tagggaattt tctattgcaa ttttataaag
    16861 agattgctat attattattt gttctaaatg attcctttaa ggtgtcacta aaaattataa
    16921 aaaatagggt tgacgcactt ttgatgtcat gttataataa cgaagtcgtt tggatgaggt
    16981 agctaataaa gcttgatttt atcgaaaaaa acttcgaaaa agttgttgac aaataacaat
    17041 aaagatgata tgataatcaa gtcgctgcta atgagtgaca aagacgttta gaaaaaataa
    17101 acaaagaagt tgttgacatt ctcttctgaa tctgataaga ttaaagagtt gttgaaacaa
    17161 aaagattaga cctttgaaaa ctgaacaaag taagacaaac caaatgtgta gggcgtcttg
    17221 attcaattca agacaacaaa catttttaac aagcaagcaa tatgctagca aacaaattga
    17281 gcttaacaat cgcaagattg ttcgaacttt ttatgagagt ttgatcctgg ctcaggacga
    17341 acactggcgg cgtgcctaat acatgcaagt cgaacgcttc tttttccacc ggagcttgct
    17401 ccaccggaaa aagaggagtg gcgaacgggt gagtaacacg tgggtaacct gcccatcaga
    17461 aggggataac acttggaaac aggtgctaat accgtataac aatcaaaacc gcatggtttt
    17521 gatttgaaag gcgctttc
//
LOCUS       DAGCWU010000047        17483 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.047, whole genome shotgun sequence.
ACCESSION   DAGCWU010000047 DAGCWU010000000
VERSION     DAGCWU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.047"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            80..562
                     /locus_tag="MBZ71_001243"
     CDS             80..562
                     /locus_tag="MBZ71_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429206.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            759..1649
                     /locus_tag="MBZ71_001244"
     CDS             759..1649
                     /locus_tag="MBZ71_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBT4429207.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(1936..3210)
                     /locus_tag="MBZ71_001245"
     CDS             complement(1936..3210)
                     /locus_tag="MBZ71_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4429208.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            3483..5954
                     /locus_tag="MBZ71_001246"
     CDS             3483..5954
                     /locus_tag="MBZ71_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4429209.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6057..7793)
                     /locus_tag="MBZ71_001247"
     CDS             complement(6057..7793)
                     /locus_tag="MBZ71_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBT4429210.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7790..8494)
                     /locus_tag="MBZ71_001248"
     CDS             complement(7790..8494)
                     /locus_tag="MBZ71_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4429211.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8625..9128
                     /locus_tag="MBZ71_001249"
     CDS             8625..9128
                     /locus_tag="MBZ71_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBT4429212.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9088..9426
                     /locus_tag="MBZ71_001250"
     CDS             9088..9426
                     /locus_tag="MBZ71_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBT4429213.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9447..10865
                     /gene="ffh"
                     /locus_tag="MBZ71_001251"
     CDS             9447..10865
                     /gene="ffh"
                     /locus_tag="MBZ71_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBT4429214.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10977..11555
                     /locus_tag="MBZ71_001252"
     CDS             10977..11555
                     /locus_tag="MBZ71_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBT4429215.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11559..12080
                     /locus_tag="MBZ71_001253"
     CDS             11559..12080
                     /locus_tag="MBZ71_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4429216.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12159..12713)
                     /locus_tag="MBZ71_001254"
     CDS             complement(12159..12713)
                     /locus_tag="MBZ71_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBT4429217.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12966..13241
                     /gene="rpsP"
                     /locus_tag="MBZ71_001255"
     CDS             12966..13241
                     /gene="rpsP"
                     /locus_tag="MBZ71_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBT4429218.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            13253..13504
                     /locus_tag="MBZ71_001256"
     CDS             13253..13504
                     /locus_tag="MBZ71_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBT4429219.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13629..14420
                     /locus_tag="MBZ71_001257"
     CDS             13629..14420
                     /locus_tag="MBZ71_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429220.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14590..15111
                     /gene="rimM"
                     /locus_tag="MBZ71_001258"
     CDS             14590..15111
                     /gene="rimM"
                     /locus_tag="MBZ71_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBT4429221.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            15101..15862
                     /gene="trmD"
                     /locus_tag="MBZ71_001259"
     CDS             15101..15862
                     /gene="trmD"
                     /locus_tag="MBZ71_001259"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBT4429222.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15998..16345
                     /gene="rplS"
                     /locus_tag="MBZ71_001260"
     CDS             15998..16345
                     /gene="rplS"
                     /locus_tag="MBZ71_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBT4429223.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16603..16896
                     /locus_tag="MBZ71_001261"
     CDS             16603..16896
                     /locus_tag="MBZ71_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBT4429224.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5770 a         3147 c         3581 g         4985 t
ORIGIN      
        1 aaaaagtttt tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa
       61 gtagtttggg ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt
      121 ttggtttttt cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc
      181 ggagatatta ggcctgatcc aaggaagcgg atttgtggtt tctaggaatt ttccagcttt
      241 gattgttttc tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca
      301 ctacaattgg cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt
      361 gctgcctcgc ttgattatcg gccctaatgg tttgagcgag agtggattga aggatttagc
      421 ttattcctcc atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt
      481 tattcttgca acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt
      541 atctataaaa aataactatt gatggtggat catcagtaaa aaaacatatg agctgcgtaa
      601 aatgcatgtt tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca
      661 tgatttttac ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac
      721 tgataaactt tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc
      781 taggtatgat gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct
      841 ctgccacaca aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt
      901 cgacagcatt acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa
      961 attatcaaat ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg
     1021 gagcagttac tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt
     1081 caaaattagt ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata
     1141 cagatgtcaa agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta
     1201 actggaagga agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca
     1261 gcggtacccg cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc
     1321 aagaacaaga ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca
     1381 gctatttagc tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag
     1441 aaccaaaaga agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt
     1501 acacgaacgg aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag
     1561 aaatccaaga aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag
     1621 aacgtgatca cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta
     1681 ctattaagga aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa
     1741 atagttcctc atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc
     1801 gtttattcga aaaaaagagc tacgacatag aactgtcgta actttttttg gctcttcgtc
     1861 aaatcgtgtt ggtgaagaca aatttgaata aaacttagtc cgatatgcgg cgcgttctgt
     1921 gcgccgcata tttttttagt tttcaaagat cagaccttgt tgtaaataga tccgtgcccg
     1981 aaagttataa aagttgcgat aaccaaacgc aattcgttta atcagcttga tcttgttatt
     2041 cagcccctca gtgacgccgt ttgagtggga caccttaaag gcatttgaaa tacccgattg
     2101 gtagcaatgg aagatcgtga atttcttttt aaacgactga ggtaacgtag atgaaattgt
     2161 tttaagggtt tcttgaaagc cttccgtacc tcgtttaacg aaataatagc gtaataattg
     2221 acaaaaatga tacgcagttc gcaattcttc gttgtagctt aacagctcat caacgatttc
     2281 tgtttgacta attggtcgtt taaacagtcg atgataatgc cgtttgagtg gctcccatgt
     2341 cgtggaatct ttgagtaaga gtttccaata tcgcttgagc cgacggtact tcttctgatt
     2401 ttcagagtgt gaggttcgaa aacgattcat gatctggaca cgtaattgat tgaagctgcg
     2461 attgatgtgt tggacaatat gaaagcggtc agttacgatc tccgcatttg gaaagactgt
     2521 tttaagcaac tgacaatagc tggcattcat gtccatcact aggtacttga cctttaaacg
     2581 ggcctttcta ggaaagcgca ggaagtattg agttagtttg taaagtctgc gatcttcaag
     2641 gatcgtaaat agctgattgg ttgtcccatc aacacaaata aaactcatgg aaccagaaac
     2701 agaattcatt gatttgaact catccataca caacaccgtc ggaaggaagt tcatacgtgg
     2761 ctggtaatgc ttcgttgctt cacgcaagat ccgtgtcacc gtcccatccg agacgaagta
     2821 cttcttagcg attagtttac gtgattgatt ttcgtacagt tccatgagaa tcgcctgctt
     2881 cagttccttt gttagttgat gatgttcatc aactaagtcc gttttcgcag aaaaggtcct
     2941 tccacaatca tggcagagaa aacgggtacg ttttaattct aaaatagtta acttttcttt
     3001 gactttcaac atctgagtgc gtgtcgtata ggaaccgttg cggataatac gaaccgatcg
     3061 acagtgttca caataggaag aagagtcatc caattttcct ttaataaact gcgcttgaac
     3121 gcctttatat tctttttcaa tcagccattc ttcatcaaaa aataaatgtt tatctgttaa
     3181 tccgagtaat tttctagtat gattatccat tgagagactc ctttggtagg atgacgtttt
     3241 ggtcgactaa attttaccaa aagtgagtct ctttttgagt tgaaacgaag aaaaagtgtt
     3301 agtagaaaat cgccttcgat tttccaccaa cacaaaatat tatagaaccc tttttttttg
     3361 ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt tccatatctg
     3421 ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat cgaggtagcc
     3481 acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa agatcatcag
     3541 ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat caagtcaagg
     3601 aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa acaaatcagc
     3661 aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc ttagccattt
     3721 tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc atagccaata
     3781 caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa ctaaatgttt
     3841 taaatacagc aaaagtcaat gtcatacgag acgggataga acaaacaata gatatagaag
     3901 accttgtaat agatgacatt gttattttag agactggata tcaaattcca gcagatgcag
     3961 aggtcgtgga agggaaactg caagttaatg aggctttatt gacaggcgaa gcagatgaaa
     4021 taaccaaaga aataggtgat ccattaatgt ccggcagttt cattgtttcc ggaaaagctt
     4081 acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca gtcaaagcca
     4141 agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa ttgatcaaat
     4201 ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt tactttttta
     4261 atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc ggtatgatac
     4321 ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact aaactagcaa
     4381 agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg gtaaatgtcc
     4441 tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa aaaatgatcg
     4501 tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc attgctgact
     4561 atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca tattttacaa
     4621 aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa aaattcagca
     4681 gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt ttgcgcgatc
     4741 aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat cgcgtgttgg
     4801 tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca gtcacaccat
     4861 taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca tttgattact
     4921 tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc accgtatcca
     4981 atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca tctaaattat
     5041 cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc gtcaagccag
     5101 aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct atgacaggtg
     5161 atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg atggcttccg
     5221 gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac ttttcacgca
     5281 tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga tcagccagct
     5341 tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg atctttgctc
     5401 tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact atcggacttc
     5461 catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa ttcatcatga
     5521 atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc ggtgcgttgg
     5581 tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca tctaccatgc
     5641 ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg aataaattca
     5701 gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc tttttgcata
     5761 agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc attctatttt
     5821 tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg tttgaacgta
     5881 aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta tcgatattta
     5941 aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac cactcgtgta
     6001 tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc tcttttttac
     6061 attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc
     6121 gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc ggtcccgcct
     6181 gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg ttcctgatcc
     6241 tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc tgttgcctga
     6301 tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc aatcagattt
     6361 ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt tccaatcaat
     6421 cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat ttgctggaaa
     6481 taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt tgacagctgg
     6541 atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa
     6601 gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt aaaaccaatc
     6661 agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc cttttgtagc
     6721 ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg tccggtacct
     6781 tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc tgtaagggtg
     6841 atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg aatgagtttt
     6901 ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac gtttaaatgt
     6961 agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat caggaatacg
     7021 ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt acacgcccga
     7081 tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc ttcccattga
     7141 tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt ttttagtacg
     7201 ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat cattcctgca
     7261 agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact ctcagttctc
     7321 ggtaaaaaaa cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg gccacttttt
     7381 ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt gcgcaaagcg
     7441 ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt tcgctgttca
     7501 ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat taaagtaatc
     7561 cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc tgcttggagg
     7621 ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc
     7681 tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa
     7741 ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt catcgtttgg
     7801 gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg tgttttggat
     7861 ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg
     7921 cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca cggtcttttc
     7981 gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc agatggtcgt
     8041 ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat agatattcag
     8101 gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt gctttcatac
     8161 gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca cctatttcta
     8221 ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc ggtgtttcat
     8281 ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga agcatcacat
     8341 ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg cctccatctg
     8401 tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag
     8461 aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat
     8521 tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg
     8581 tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg
     8641 catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga
     8701 tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac
     8761 caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc
     8821 aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga
     8881 tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa
     8941 gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata aaaaaaaatg
     9001 gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag
     9061 aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg
     9121 ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat
     9181 tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca
     9241 gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaactccat
     9301 cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca
     9361 gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa
     9421 gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc
     9481 aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga
     9541 tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt
     9601 ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg
     9661 cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag
     9721 cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg
     9781 caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc
     9841 gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat
     9901 taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga
     9961 tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata
    10021 cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat
    10081 tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca
    10141 acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg
    10201 atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca
    10261 aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg
    10321 ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact
    10381 acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca
    10441 atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca
    10501 aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag
    10561 atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg
    10621 atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg
    10681 aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca
    10741 aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac
    10801 taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga
    10861 aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct
    10921 atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga
    10981 gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg
    11041 ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg
    11101 gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat
    11161 tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca
    11221 aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct
    11281 tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa
    11341 aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag
    11401 atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct
    11461 atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag
    11521 aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc atattttatg
    11581 gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa tggctttttg
    11641 acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc gatacgacca
    11701 tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa gcaatttatc
    11761 cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca gatgctgagg
    11821 tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa ggaaaagata
    11881 tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa atgattgatg
    11941 aatggtatat ccagattacc cctgtattat taggggacgg gattccttta ttccaaaaag
    12001 gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa tttatcgaat
    12061 tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat gcagaaatgt
    12121 cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa tttgttagaa
    12181 aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc aacgatatta
    12241 ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt
    12301 ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa gccgccgatc
    12361 ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact gtgtccataa
    12421 ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg gttgtattca
    12481 ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg agtccattca
    12541 gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc atcgattttt
    12601 acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc tacttttttg
    12661 ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt catacgattt
    12721 ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga gcaaataaaa
    12781 acacttactg acaaaagcta catagaagat agattactta tttttaacaa aaaagaagtc
    12841 taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt
    12901 tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg aggtggaaat
    12961 aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt ccgttttacc
    13021 gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa actgtaggta
    13081 cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta gttcttgact
    13141 ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca aaagaaggcg
    13201 ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg atatgaaaga
    13261 tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg atcaagtcaa
    13321 gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg ctcctgaaga
    13381 tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca caatcgttta
    13441 cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa
    13501 gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt tttattaatt
    13561 gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga ggagggagtg
    13621 aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta agcgcttgta
    13681 gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct gaaactgtta
    13741 ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca atggctacct
    13801 caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca aatgttccca
    13861 tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca catacaaagc
    13921 aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat gctgacgaat
    13981 tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct tctagtcaag
    14041 aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa gtggacttag
    14101 gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt gaatggcaag
    14161 aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat cccttactat
    14221 tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct gaacaatttg
    14281 gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta agctggcaag
    14341 aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg aagatggcag
    14401 tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt aactccaagt
    14461 cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct taatacctta
    14521 aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa aaagaaatgg
    14581 ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa
    14641 ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa aaaaggaacg
    14701 gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa aagtcatcgc
    14761 aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa tgatgtggaa
    14821 aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct agctgaaaat
    14881 gaattctatt accatgagat catcggtctg catgtattag aagatggaac tgagttaggt
    14941 aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca acgtccgaaa
    15001 aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga tttagaagct
    15061 ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg atgtattgac
    15121 tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta aggctgtaga
    15181 taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata acaaacatca
    15241 gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag tccagcccat
    15301 ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct
    15361 tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt cacaagaaga
    15421 acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc gcacattagt
    15481 gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag gtgcgatggt
    15541 catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga cttcagctca
    15601 aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc cggcagaata
    15661 taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga ttgaagaatg
    15721 gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt tggaaacact
    15781 tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag aacaagaaaa
    15841 aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg ttgataatat
    15901 gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg ctgtgagaaa
    15961 agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa tcgaagaatt
    16021 aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt
    16081 tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag gtgttgttat
    16141 caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt ctaacggagt
    16201 gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg aagtagttcg
    16261 ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg gaaaagctgc
    16321 tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa cccttggtat
    16381 tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt tttctttaat
    16441 tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta gtaaaaaaaa
    16501 gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc cagaatattc
    16561 taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta tacttatacg
    16621 gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt aaaacaagta
    16681 ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg ctttgaccat
    16741 ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt ttctagtggc
    16801 gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag aagttcagac
    16861 ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat actcattgaa
    16921 aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt ttgtcatctg
    16981 tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga taaaagctaa
    17041 tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag cgtacatgaa
    17101 aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt acgaataaat
    17161 accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa gataacaaga
    17221 tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa acagtatttt
    17281 gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac cattttttaa
    17341 gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca caacactgca
    17401 gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa gaaaaatagt
    17461 agataatccc gtcctaactc ttt
//
LOCUS       DAGCWU010000048        17350 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.048, whole genome shotgun sequence.
ACCESSION   DAGCWU010000048 DAGCWU010000000
VERSION     DAGCWU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.048"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            51..863
                     /locus_tag="MBZ71_001262"
                     /pseudo
     CDS             51..863
                     /locus_tag="MBZ71_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            876..1604
                     /locus_tag="MBZ71_001263"
     CDS             876..1604
                     /locus_tag="MBZ71_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4429225.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(1673..3199)
                     /locus_tag="MBZ71_001264"
     CDS             complement(1673..3199)
                     /locus_tag="MBZ71_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBT4429226.1"
                     /translation="MKRLIGILAMLVLAGMLTACGGASGKAEDSKEKLSVMTTFYPMY
                     DFTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQK
                     TLQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVAN
                     IRDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLAL
                     EYGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGV
                     SLEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNG
                     YFEDEDVQDRSLSDWAGTWQTVDTYVEDGTFAPVFEYKEKLNQDKTAQEYKEYYTKGY
                     QTDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGA
                     FRYIQFRDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH
                     "
     gene            3451..4632
                     /locus_tag="MBZ71_001265"
     CDS             3451..4632
                     /locus_tag="MBZ71_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4429227.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(4692..6320)
                     /gene="treC"
                     /locus_tag="MBZ71_001266"
     CDS             complement(4692..6320)
                     /gene="treC"
                     /locus_tag="MBZ71_001266"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBT4429228.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRITIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(6717..9428)
                     /locus_tag="MBZ71_001267"
     CDS             complement(6717..9428)
                     /locus_tag="MBZ71_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4429229.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEHNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWSYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(9438..10139)
                     /locus_tag="MBZ71_001268"
     CDS             complement(9438..10139)
                     /locus_tag="MBZ71_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429230.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            10324..10926
                     /locus_tag="MBZ71_001269"
     CDS             10324..10926
                     /locus_tag="MBZ71_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4429231.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            11007..12020
                     /locus_tag="MBZ71_001270"
     CDS             11007..12020
                     /locus_tag="MBZ71_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBT4429232.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            12129..13046
                     /gene="rbsK"
                     /locus_tag="MBZ71_001271"
     CDS             12129..13046
                     /gene="rbsK"
                     /locus_tag="MBZ71_001271"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBT4429233.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            13074..13469
                     /gene="rbsD"
                     /locus_tag="MBZ71_001272"
     CDS             13074..13469
                     /gene="rbsD"
                     /locus_tag="MBZ71_001272"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBT4429234.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGSEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            13469..14842
                     /locus_tag="MBZ71_001273"
     CDS             13469..14842
                     /locus_tag="MBZ71_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4429235.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPIMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            15199..17343
                     /locus_tag="MBZ71_001274"
     CDS             15199..17343
                     /locus_tag="MBZ71_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4429236.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         5201 a         3434 c         3195 g         5520 t
ORIGIN      
        1 aggaactttg agatattctt taggatatta aaattaacta gcaccacggg tactatatca
       61 ttatttttta tacccgtttt tctggtgaaa cacctgatgt cttagctttg aatacgagtt
      121 ttttcattct atatctgtgt gttggcctga tcatcttggt ttattattgg aaaatctcta
      181 ataagaaatt agctgatcag ttattagagc aacaagtcag gatgcaacag gattatatca
      241 aagatctaga aaaaaactat caagacttga gagaattcaa acatgattac caaaatctgc
      301 ttttttctct tcacagctat atttcagaag gcgacttaga aggattacgg tcttattatg
      361 ctcaaacgat attacctaca cgccagatga tgaacctttt tccggccaat ctaagtatgt
      421 tggataatat gaaagttcct gagatccgta gtttactgtc attaaaactc atgatggcac
      481 aggaaaaagg gttggtcgtc cagcttcatt ttcctgagcc aatcgaactg gatacgaaac
      541 atacggtaaa ccttgtacgt atgcttggta ttatcttgga caatgcgatt gaaggagcta
      601 gtgctgcaaa gaagaaaaaa atcgaattga ccatactcaa gaagaatcat tccgtgatga
      661 ttcgtgtgat caatacaaca acaaatacat tgccgttaaa ccagttgaaa caaaaagggt
      721 tctcaacaaa aacaaatcca aaaaacgaag gccttggctt gtttatcttg gatgaattga
      781 caaagaccaa tccttatctt ttccttgaaa catcaattga aaaccagcgt ttttctcaaa
      841 cgatttatat acaaataaaa taaaggagta atgatatgat tcccatttat atttgtgaag
      901 acgatccaaa acaattagaa gttttatcaa cggtgatcaa aaatagaatc atgattgaaa
      961 acttagacag ttatgtccat atttctacct ctgatccgga agaacttctc aatgcagcca
     1021 agatcagaac ttcttctttt ggtatttact ttttagatat cgaactagaa gacagtgaaa
     1081 tggatggtgt agcgatcggt aaattgatcc gtgaattaga tcccttagga aaaataatct
     1141 atgtgacttc ccatacggaa atgtcgatga aaatcttaaa aagcaacatc gaaccgactg
     1201 attatatcgt caaagaagat ctttatgatt taaaagaaaa tgtcgagcat atcctgagca
     1261 aaattttcga agattttcaa atgtcccaga tccaaaaaga tatcttcaaa atagagttta
     1321 atgatgagat caaattttta ccgatcaaag acatccaata tttttctact tcgctaggag
     1381 caccacataa actggaagtc catttgactc atgcccaatt acaattctat gagaagatca
     1441 aagaaatcga gaaaatgcat cggaatttta tccgttgtca taaatcctat gttatcaata
     1501 cacaaaatgt gcggtcgatt aataaaaaaa ccagagaagt cacgttggca aacggcgaga
     1561 ccatcccagc ctctatccgt ggattaaaga aattgatttc ataaaaagaa ccgaatagtt
     1621 tggaccgact aaatgactag ccgatccaaa cccttcggtt cttttttgcc tatcagtgca
     1681 aaagcatttc ctgtgcaatt tcacgtccat ccattgtatc tggataaaat gttggccaat
     1741 tctccatttc tttcagcaat gcttcttgac tttcatttcc tgcatataaa tgaaaatgtt
     1801 cggagtcagc cggtgcaatc gtgtgatcac ggaattgaat atatctaaaa gcgccacttt
     1861 ccttatcttc tgcttcgaat agatacctta cgccacggtt tcctgcctcg taggtcaaga
     1921 tttctttccc tacataacga taatttgaac gtttcgttgt tccatcctta aacgtaaatg
     1981 ccatctcttt ctcactgatc ttgattgaag caatatctgt ttgatagccc ttcgtataat
     2041 attctttata ttcttgtgct gtcttatcct gattcaattt ttctttatat tcaaaaacag
     2101 gagcaaaagt cccatcttct acatacgtat ctactgtttg ccaagttcct gcccaatctg
     2161 aaagagagcg atcttgtaca tcctcgtctt caaaataacc gttgtagacg ctcttttctt
     2221 ttttgggggt caaagtatct tctagcgggt tttctgtctc tgtcgttttt ttcaacgctt
     2281 cgaggtttgc ttccatgata gaaagatagt tttctccatt ttctatttgt tcattcgtca
     2341 atccttccaa aggattcaat acttctaaag atacacctgc ttcgtttgct aacgttctgg
     2401 caatactatc tttggcgttt tcttcaaaat aaatatagtt gattccatgt tctttaacaa
     2461 agtgtttcag ttcagcaatt ctcgccgcag acggttcttc actagaagaa agaccagcaa
     2521 tcgatacttg attcaatcca tattctaatg ccagatagtt gaatgcagca tgttgcgtga
     2581 caaaagatct ttgttttgct tgcttaaagg tttcttgata aagctgatgg agtgcttgaa
     2641 gtttctcggt ataggctgct gcattttttg tccatacttc ctgtttttct gggtaagctt
     2701 caatcagctg atcacgtatg tttgctacct gcttgattgc taaacttgga gctagccata
     2761 catgtgggtc tagctcatgt gtatggcctt cttccccgtg ttcatggtca tgatcatgtc
     2821 cttcttcact tcccggaagt aaaagcatac cttctgtcgc ttttatcgta tggacattgc
     2881 cttcttgtaa tgttttttgg attgcgggga cccatgtttc catgttttca tcattataaa
     2941 caaagatatc tgtatcttgg atctttgcca tgtctttagc agaaggttca taatcgtggg
     3001 aatcagtccc tgctggaatc aataactcta cttctccttc atccccgacg attgcttttg
     3061 taaaatcata cataggataa aaggtcgtca tcacgctcaa tttttcttta ctgtcctcgg
     3121 ctttcccact tgccccgccg caagcagtta acatacctgc taaaaccagc atggccaata
     3181 tacctatcaa tcgtttcatt tatgacactc ctcttctcat cttctttcat ttgaatgttc
     3241 cgattcgcct tttacaaaac gtaatagtta cgttttagca acaggattta atttatcata
     3301 tggttaaaag aactgtcaag cgttttctta ttttaattga tgagaaagtt tatatgaatg
     3361 attgacgata tttttgcaat caacctctat cattttcctt ttatacccgc ttttgtctat
     3421 aatgaagtta tagataaagg aggaaaaaga gtgaaaaaag aaacagatac aacgaatcgt
     3481 ttgatccgct ttttaggagg aaaaacctct tactatgttt taggaatagt tatcttactt
     3541 gcgctgatca tttatttttt ccatcagatc tcgtttatat tcaagcctgt cggaatcatt
     3601 atcgctacta tcttgccccc attattgttt gcactgatct tgtattatct tttggaacca
     3661 gtcatcaaac gtttgagcaa gcacctttcg aggaacttgt cagtcattat cgtttatgtg
     3721 attattttat ttttattggg gcttggaagc gtatggcttg ttccttttct tgaagaacag
     3781 gcaaagacat tgatcagtag tttgccagat ttgtttcaag attttcaaga gagtctccga
     3841 caatttttag agaaaacacc ttttgccgat tcatttaatc aatttttcgc ttctattgac
     3901 gacgtgacgt ctgatatagc tggatttatt ggaaattatt gggaaagtgg cgcacaacgg
     3961 atcagcaaca ttttttcaac tgttacagct atttttatta ctttatttac tggtccaatt
     4021 gtcgcttttt ttcttttgag agatcctcaa aaattttatc agtcagtttt agcgattgtc
     4081 ccaccagcat ttcgtacaga ttttaaaaat ttgacaaaga ttgctaatca acaattgggt
     4141 tcatttctaa aggggcaaat cattgcttca tttatccttg gagcagtcta ctgggtttgt
     4201 ttcttactga tcggattaga atttgctagt gttttagcta tttctgctgg gttgttatgt
     4261 atcattccat atattgggcc gtttatcgcc ttctttccag gtttgatcat tgcattccaa
     4321 gactctactt ttatggctgt caaatttgtc attgtctggt ttgccgtaca acttctccac
     4381 ggtgacttgg tgattcctcg agtaatgggt gatcgtttac agatccatcc gattacgatc
     4441 ttaatcgttt tacttgtcat gggtgatcta atgggaattg tgggcgttat ttttggtatt
     4501 cctatctata cgttagtcaa attactcgtt acttttattt tccgtaaatt caaacaacgc
     4561 tacaataaat tttatggcga taaaggagag tatgaaaact cttcattttc tgaagaagat
     4621 tattttaaat aaaacacaaa acgtcgatct tacgagaata gctcgtagga acgacgtttt
     4681 gtgtttttgt tttaattttt cagcaaagca attgcctggt atggttttag ctccagactt
     4741 tgatgaagaa cagtatcgga ataattatcg ataagaatat tggcttctgt gaatccttct
     4801 gggatggtga tccttgtatc tttatcatag aaatttgtta atacaagcag ctgctgtcct
     4861 tcaaattggc gaagaaagcc ataaacttcc ggatgatttg cttcataagc atgataatct
     4921 ccttgtgcaa cgattggata tgtttttctc agccgaatca atttcttgta ataggacaaa
     4981 atagaatctt cagaagatag ttctttttct acattaatct cttctagctt gcctccaact
     5041 tttaaccaag gtttccctgt agtaaatcct gcttgttctg tgtctgacca ttgcatgggt
     5101 gttcgagagt tatcgcgaga ttttgctttg atccgacgaa acgcttcttt ttctgtgaat
     5161 ccttgttcaa ggagtaactt ataagcattc aaactttcta cgtcaatata gtcggaaatc
     5221 gatgaatagt ctgggtcgat catgccgatt tcttctccca tataaatata gggcgtccca
     5281 cggttcaaat ggatcatggt tgctagcatc tctgctcctt gttttcgata ctgatcgata
     5341 gctacaaatc gattgagcgc acgtggctga tcatgattat taaaaaatag agcattccag
     5401 ccgttaccct cactcatctt ttctccccat gtatgaaata gtcgctttaa ttctgcaaaa
     5461 tcaaatggag gagtagacca tttattccca ttttcataat cgactttcaa atgatggaaa
     5521 ttaaagacca ttgataattc ttctctacta ggttctgtat acaagataca attttcgatc
     5581 gtcgtagagc tcatttcacc gactgtgatg atagtggaat ccgtcccaaa cgttgcttgg
     5641 ttcaattctt ttagatacgt atgggtgatt ggtttgtccg tatattcagg tttgccatca
     5701 ttttctttgt tatttttcaa ttgttcgtct ttaccgataa cattgataac atcgaaacgg
     5761 aatcctttta cccctttttc catccaaaaa cgaacaactt tgaatagttc ctctcttact
     5821 gcttggttcc gccagttcaa gtcagcttgt gtccgatcat ataagtgcaa ataatatttc
     5881 cccgtatctc caaatggcga ccaagcactt ccaccaaatt ttgataccca atccgttggc
     5941 tcttctctta aaatgaagaa gtcctgataa tgctggtccc ctgctagtgc ttttttgaac
     6001 cattcatgtt ctgtcgaaac gtgattcagc accatatcga gcatgatttc tatccggtat
     6061 ttctttgctt cggctattag ctgttcgaat tcctccatcg taccaaagat aggatcgatg
     6121 gctgtgtaat cagagatatc ataaccgttg tctttttgag ggcttggata aatagggttt
     6181 agccaaatca tgtcgatgcc tagatcattc agatacggga gtttttcttt gattcctttt
     6241 aaatctccaa ttccatcacc attactgtct ttgtaagatt tgggatagat ttggtaaatg
     6301 actttgtctt taaatgtcat ggatatttac ctccattcaa ctgatttatt acctaccgca
     6361 acttatatat acaagttatc atttattatt ataactgaac agaaagcgtt tgtaaaattt
     6421 atctttatac attctaagag gtctgttgcc caataaaaaa caagagatca gacatgtatt
     6481 tgtctatcct cttgtttaaa atagcaaaat attcttgttt cttctataaa tggtgctcaa
     6541 aagcttttat ctacggcaat cagtcttctc aaatgtaaaa catgaggagc taattttatt
     6601 tgagtattcc ttttgctcga tgcaggacag ctttttcaca acctctgatt cacggtgttc
     6661 aattagcagc tgaccctcgt tattagctca aaacgctgat ttcacaacct ctgtttttat
     6721 tcattcgatt tcaacgcatc aatcatatcg atatgcttta atttgaaatg ggtaacaatc
     6781 atcacaataa ccgtaaatac gatagttaat cctgctgata agacataaga ccaccatgtg
     6841 atcactaatg gaaagatgac attctccata gacgcttgtt gcaagatgaa gtcagtcaat
     6901 atatatccta caccaaatcc tccgatgatt cctaaaagag taaagatgat attttctcga
     6961 acaatgtaca tcgtgacttc tttatcaaaa aatccaagga cttttattgt agataattcg
     7021 cgtacacgct cggaaatatt gatattagtt agattataca agacgataaa agccagcagt
     7081 cctgataata cgacaaagat taagacaacc gagtctaagt tcgccattga ttcttcttgc
     7141 gtttcgattt gtttggacat gaaagacgta tttgtcacag cgtctgtggc aagcagtttc
     7201 tcggaaagca tttcttcttc ttttgttgtc atcttatctg tacgcagcaa aaatgcatta
     7261 tccatcatcg attgatcagt aactgaagca taatattcag gggacatata aataaagcta
     7321 cctagatagt ttttcacgat ttcttttact tcgactttcc attctttttg atcttcatca
     7381 taaagcgaaa tatgatctcc tgtagataag tcaaatagtt ctgctgcttt ttgcgtaata
     7441 gcgattcctg ttttaggcaa ggagcgtggg ttctcttcaa gatcagttaa atggataaaa
     7501 cgatccaaat cagctgtttt acttggtacg atcactgtca gactttgcga agcatgtcct
     7561 ttttttctca attctacggt ttgagtatga atagctagga tctcatttac tttttcttgc
     7621 ttctgcagca tatccgctgc tttttgacga ctatcttccg tggatgaatc caatgtaacg
     7681 attgcttgat agtcaatgat cggtccaaat tgtttagctg agacagaact tagagaatct
     7741 ttcaatccca cgcctgcaac catcagcccg gcacatccag caattccgat aatcgccata
     7801 agcattcttg acttatatcg gaagagattt cggtaactga ttttttgatt aaagctaagg
     7861 cgtgaccaca gcggagtgat gtattcaaga aaaatgcgtt tccctggctt cggtgctttc
     7921 ggttgaagca atgcagcagg ccgttctctt aaatctttga ataaaacgat cattgcagcg
     7981 ccaaatgcgg caatgaagaa tgcggcagct gcttgtaaaa tcggcggcca gtcataaaaa
     8041 ataagcgcac tcccaatatc ataccgctcg ttagacagct caaaaatgat ccttggcaaa
     8101 agattcgttc ctaatatcgt tcctaaaaca atgcctatcc ctgaggctag acttgcatag
     8161 attgcatatt tttttgcgat ttcaactttc gtataaccta acgcctttaa tgtcccgatt
     8221 tccctgcggt tttcttctac catacgtgtc attgtcgtaa aggtgatcaa tgcggcaata
     8281 aagaaaaaga aaacaggaaa aacattggca atcgcagcga tacgatcaga cagatcacca
     8341 aattcttgaa aaccgacatt ttctgaacgt tcattgtata ggtatgatgg tttagttaaa
     8401 ttttcaattt tttctgcatt tcgattcaat tcttcctctg cagaggccag ttcacgttct
     8461 ttctcaacta attgcctttc ttgttcagca agttgagctt cttgttgaga taatgtttga
     8521 acagcgattg ttcgttgcgc ttcaggaagc tgctggatag ctgctgactg ttgcgctaat
     8581 tgttttttag catcttctaa ctgtgttctt ccctgtgcta tttgtgcttt gccttctgtt
     8641 attttttgct gtgctggttc taattcttcg tttgccgatt ttttcagctc ttctaagcgt
     8701 tcttcagggc gatcttgtaa atacttttct gccttctctt gattgtgttc cattttatct
     8761 ttgtaagctt tgctgtacgt attcaaattc tttacgtttg taaaatcaag atataagaca
     8821 gaataaatat ctgttttaaa aacggattca ggtaaataga caaaataatc gacgctacca
     8881 tttccgacgt tcgcgtatcc tctctccaaa ttgttgatga acaatggaga acggacaaag
     8941 cccgtgatgg tatactcttt ttctttcaat tcatctgaat caatctgata aatatcaccg
     9001 attttatacc cttcatcttc cgctttttca tccaatacaa gctggtttgg tttttccggg
     9061 agttttccat cagttaattg tagcttgttc tgcttgttat ttttgttata agaaaatatt
     9121 tgaatcactt cgtttttacc agtattggaa taatagatct gttttcctgt ttctactttt
     9181 gcatctggga tcttttctgc ttgagcaata tctttttgtg tcaagccacc agtagaaacg
     9241 atttgcaaat cagaaagtcc tgctgtacca acatagtggt ccatcgtttt ttgcatgaca
     9301 ggtccagcag ttttgacacc tacaaataaa agtgttccta gcatgacaat aaggatgatt
     9361 gcaatgaatc gtcctttaga ttgccgaatt tcgcgaaaac ctgctttaag atatgttctt
     9421 gttttcataa aatcccccta ccatacaatc tcatcaatgg atgaaggatg atcatttaat
     9481 tcaacagacc ttactttagc atcattaatg tgaatgactc tatcagcgat gggcgctaaa
     9541 gcagaattat gtgtaatgat caaaacggta ttgccgtgtt gtctgctagc gttttgcaat
     9601 aatttcaata cttgcttgcc cgtttcaaaa tccagcgcgc ctgtcggttc atcacataaa
     9661 agaagtttgg gatttttagc tagtgcacga gcaattgaaa ccctctgctg ttcgcctcct
     9721 gataattggg acggaaagtt attcaatcga tgagctaggc ccacttgcct caatacctct
     9781 acaggatcaa gcgcatcagg agaaacttcc gttgccaatt ccacattttc ttttgctgtt
     9841 agattaggaa cgagattgta gaattggaaa acgaacccta catctgttcg tcggtaagca
     9901 gtaagctgtt tatctgaaaa ttgcgcaata tctgtgtcat caataatgat ctgaccttca
     9961 tcaggtgtat ccatcccacc taaaatatta agaatcgttg actttcctgc tccacttgga
    10021 ccaagtataa tcactaattc gcctttttca attgaaaaag aaatatcgtc atttgctgta
    10081 atggtcgttt ctcccatttt gtatcgtttg tattcatttt ttacttcgac atacttcact
    10141 ctgattcctc cattttcacg atatttctcg ctatatctat gcatcgaatg cgttttctca
    10201 tacttattta ttttaccact gaatttctct agactaaaga aaggaatcct ttatttagtt
    10261 gacaaataaa cgatttatga tttacttaca taaaaaacat tgttatttga aagaaggaac
    10321 atcatgaatg atttaaagga aaatatgatt tttgatttat caaaaactgc ttttgtcgcg
    10381 atcgacatgc aaaaagggat cgtaaataac ggtacactct ctccacacac tgcacaatct
    10441 attctgacaa acaatgattt gttagcaaaa acattgaaac gtaccgctgc cttgaaagtt
    10501 ttagtgaatg tagacatcac tactttctct tatctaagtc atcagacaga tgctggagga
    10561 atatctgctt tgtctgctcc ggcagaattc actgacttgt tgttgaagga ttccttgaaa
    10621 gatacaaaca atatgttgac tatcacaaaa tacaatccaa gtgccttttt tggtacttct
    10681 cttgatcttc aacttcgccg gcgtggaatc gaaactatcg ttttaagcgg tgtagcgaca
    10741 acaaacggtg tatatgctac tgcattagat gccttccaac atgggtatca tgttgtactt
    10801 gctgaagatg cttgcagtga tcgtgacaaa gaatcccatc aattgttcat aaaaaagata
    10861 tttccgaaaa ctgcacgagt acgctcaacg aaacaaataa tcgaagctat ccaacaaagc
    10921 aaataagtac tggcacacgt gattttcatt tacttctttt tgatttcatg ggaaaataac
    10981 cacaggattt agataggagt atgaacatgg tgaaaaaaaa gatcacaatc aaagaagtcg
    11041 ctaaactttc cggcgtttca atcacaactg tctccttgat attaaatgga aatacggaga
    11101 aattcagtcc gaaaactgtt cagaaagttt tagctgccaa agaagaatta ggctatcagc
    11161 ctgactattt tgctagacaa atgattacca aggaaacgaa aacaattggt gtactcgttc
    11221 ctgatatcac gaatccattc ttcagtacac tgatgcgagg aattgaggat atcttgtata
    11281 agcagaattt cgtcacgata ttatgtaatg ctgattctga tcatcaaaaa gaaatcgaat
    11341 atctagccga gctcacacgt cgaggtgtgg atggattcat tatcgcgact tctgctgtct
    11401 ctacagatgc aatcaacgaa aatttgaaaa aacaaggccg gcccttcatt gttttggatc
    11461 agaaaaaatc tgaaggattt agtgatgccg tacgaacaga cgattttcgc ggtggttacc
    11521 tagcgggcat gcacttgctt agtctaggtc atcaaacgat cgcccttgtc tatcctgaaa
    11581 atccgccgga aaatgtccac gctcggattg aggggtttaa atctgctctt gatgtttacc
    11641 aaatctctca cgaccaactg atcctcttac ctacacagtt ctcaaaacaa ggtggctacc
    11701 aaatcacagc agaacttctt gattctgctg caactggtgt gtttgcttta aatgatgaat
    11761 tagcttttgg attatatcgc ggattagagg aatctggaaa aagcattcct gaagactaca
    11821 gcattattgg ctatgataat atagatatgt gcgagtatat caagccgaaa ctgacaacga
    11881 tcgcacaacc gatcttcgaa ttgggacaaa catctgccaa attattgtta gatcggatcc
    11941 aattcccaga aaaagaatgg gaagaaaaaa gattacctgt acgattagaa aaaagatttt
    12001 caactgcacc cttgaaatag ctttcaaggt gtgctatatt tttatcgaac tattaaaacg
    12061 ttttactaaa acgttttaaa aaataatttt tcccatcaaa ataaatgaga tagttggagg
    12121 tttgtaaaat gaacacggtt acagtaattg gaagtattaa tatggacact acccttcgtt
    12181 taaccaacat gcccaagcca ggcgaaacaa tgcatgcgca tgagattttt cacgctggcg
    12241 gaggtaaagg tgccaatcaa gcagtggcag ccaaacgttc aggcgcacgg acatctttta
    12301 ttggaggcgt tggggctgac agcgaaggac aacagttatt agatctactg acaaaagaaa
    12361 acatcgatac atctgggata gctgaaatcc aaggcgccac aaccggacaa gcaatgatta
    12421 tggtagacgc agcaggagaa aatagtatct tgatccatgc aggtgcaaat aatgctttcc
    12481 atgaacaaga agtcctaaaa aacaagcaac tgattacaaa cagtgacttt attattgctc
    12541 agtttgaaag tagcttagat gcgacgatcc tagctttttc tattgcaaaa gacgcaggaa
    12601 aaacaacgat cctgaatcct gcaccagctc gtgaaacgat ccctactgaa ctacttgaaa
    12661 aaacagatat cattatacca aatgaaacag aaacagaaat cattacagga atcagagtga
    12721 cagatcataa cagtttggtc gcagcagccg aaaaactcca tgaattaggt attggaacag
    12781 taatcattac actaggcagt gccggcgctt tctatcatac agaaaaagaa cacggtatcg
    12841 tccctgcttt taaagtggat gccgttgaca caacagccgc aggtgataca tttattggcg
    12901 cattaagcag cacacttcag ccagacctta gtaacttgaa agaagcgatc ctttatggaa
    12961 atctagcctc ttctgttgct gtgcagtctt atggtgcgca accttctatc ccttaccgcg
    13021 aagctttaga acaatcagca gtctaaaatt aacaatgaga ggaagaaaca aacatgaaaa
    13081 aaggaaaagt aatcaattca gatatttcac gagtaatcgc acaaatgggg cactttgata
    13141 aattaagtat cggggatgca ggcatgcctg tcccctttgg tagtgaaaaa atagatttgg
    13201 ctgtctcaaa cggtcttcca agctttttat ctgttttaga aaacgtttta gaagaacttg
    13261 aggtacaatc gattgaattg gcggaagaaa taaagacaat gaacccggaa atattggaaa
    13321 aaatcaaatt attgttacca gatacaccga ttagctttat cccacatgaa gaaatgaaaa
    13381 aagagttgaa taactgtcat gcatttattc gtacaggcga aatgacacct tacgccaata
    13441 ttatattagt gagtggagtg gtcttctaat ggaaacgaca atgaatcctg aacagatggt
    13501 actgtccgag cagattcata tgctgattga acagttggga ggaaaagaca atattgattc
    13561 tgtccttcca tgtgcgactc ggcttcgtat caaattgaaa aaacagccag tagccaatgc
    13621 agaaaaatta aaacagcaaa tcccttccat taagtatttc attttactcg atagatatgc
    13681 agaaataatc atggatcagg gattggctcc tgcttacaag gaattaacgc gtcaaatcga
    13741 tcattcagct cctaaagaaa agctaaatcc gatcgaaaaa ctatttggtt tgatatcgga
    13801 aatctttacg ccgatcattc cgctgtttgc cggttctggt atattaaaag gtctggttgt
    13861 tttagcgact acgattggct ggcttagtga aacaagcgga acttatcata ttttgtctgc
    13921 tgcgtcgaat gccgtctttt attttctgcc aatctttctt gctttttctg ccgccaaaac
    13981 gtttaaagtg aatggatata ttgcggctgt cattgctgca gcactgattg aaccgaatct
    14041 gacctcttta ctttcagaca gtggcaaagc agctactgat tttctgggga ttcctgtagt
    14101 actgatgcag tatacgtcta ctgtcatgcc ggcgatttta ggtattttct tttattcttt
    14161 tgttgaaaaa ttcctcaaaa aatatattcg cgaaaatatg caattagtat ttgtaccttt
    14221 actttcatta gttatcacgg ttcctttgac gttaatggta ttcgggcctg cgggtgttta
    14281 ccttggcgaa ggtttagctg ccgcaattac ttggatgatg gatattaacg gaccgatcag
    14341 cggcgcaatc atcgctggtg gttggaatat tttggtaata tttggtatcc aatgggctgt
    14401 aaaccctatc atgatcagta atatctctgc ttatgggttc gaccgaatcg ttccgttgac
    14461 aggagcagcc aattttggaa tggctggtgc agctttaggt gtatttctac gaagtaaacg
    14521 ttcaaaaact cgttcgatct ccggttctgc ttttgcttcg attttacttg cgggagtgac
    14581 cgaaccaact gtttacggaa ttgcgatccc attgaaaaaa ccatttgttg ctgcttgcat
    14641 tggtgcagct gctggtggag ctgtaatggg atttgcccaa gtgaaagcga tcgcttttgt
    14701 atttggtagt ctgaccacat tgcctgcatt catttccggt acttttttct ggtacttagc
    14761 aggactggct gttagtctag tcgtagcaat gattactact ctcgttagcg ggtttgatga
    14821 agacctaatg tcttatgaat agttaatgaa aaagactgaa gtatgagatg tttatcacat
    14881 atttcagtct ttttcttttt aggatcatta tttttataaa tttttgtgat actttccaaa
    14941 ctttggtata atttacatat gcataaccat ttttatcccc acttgtttat ccccaaacaa
    15001 aggtggtcta tgcaataaaa tgaacgtcaa tgaaaaacga atgaatgctc gttgcttatt
    15061 tacaaattgt tttatttaga atacacagac agatttatga aaagaaaaag cttttcgaca
    15121 cgattatctg ttccaaaaaa tgtctaatga atgctgaata agacagaaaa tcaatgaaag
    15181 gaaaaggact agtttattat gaaaaataaa aaaacgatac gtttgctagc tgctacttgg
    15241 ctgattgctc cgtctgtttt gtcgaccaat accgtatttg ccgtagataa tcaaacagct
    15301 gatcaaacag tcacaacaga tacgactaca tccagctcag ctactagtga aacaacggat
    15361 tcttttacag aagacccaac tggaacaact aactcaacaa caggatcgac cgagccaact
    15421 gaaattgatc cggctgatcc atcagaaaat gaaaagccag ccgagacata ttcccttaca
    15481 attgctcctt ctttgattgg taagatccaa ctgactgctt cgacagcgga ttcccctgaa
    15541 cagcaacctt tggatattac ggaaagtgga acggtagaag gtttattagc ggaaacgaag
    15601 gtaacttaca ctattacgcc agctgaaggt gaacaaattg cttcagtcgc tgttaatggt
    15661 gccaaagaag taaattatga tgcaaagacc tttacagtag gtacgaacaa tttatctatt
    15721 actgcagaat ttgaacctgt accggatact tcaacagaga aaccaactga tcctagtgaa
    15781 gaagtcccta gtacaccaga tccggaaacg ccaaacaaac cagatgtggg taataacaac
    15841 aataataatg gttcaaataa ttcaaacaat aataacaata attcgaacag cgggaattct
    15901 tcgaatcaaa caaacccatc tggatcgaat aacaatggaa atactcagag agaaccaaac
    15961 catccaaaca atggttcatc aaatggcgga acgaacaatg cttcatctcg tccaaataca
    16021 gcaaatcaaa gcggtataga gaatccatct tctgcttctt ctgattttgt tgtaaaatca
    16081 ccaattgatg ctgtattgcc tacaaataca acgaatgtcc aacaagcaat cgttcgagaa
    16141 gcattcaagc atcttggaaa accatatgta tggggagcaa aaggtccaaa tacttttgac
    16201 tgttctggtt tgacttatta cgtatatatg aaagcaaccg gtcattatat cggtggctgg
    16261 acaggcgaac agcaatatgc aggtacacag attccagtaa gtcaggccca acctggtgat
    16321 ctcgtcttct ggggaccttc ttctggtgtt acccaccacg taggtatcta tattgggaac
    16381 ggacaattta tccatgcgcc tcaaccagga gataaagttc gagtaacttc tatttcagat
    16441 ttcacgcctg attttgctgt ccgagtaaac ttagcaggtt tacctgctgc ttcaggttcg
    16501 ctgacaggcg gctcatctat tttagatgga ttagatggct cgttccattt cagtcaaaac
    16561 caaacaactg atcagttctt gaaaaagatt gcagatgatg cacaggaaat cgggcaaaaa
    16621 gaaggaatct acgcatcagt catgatggct caagctatcc ttgaaagtgg atctggaaac
    16681 agcttattat caagcgaacc aaatcataac ctttttggta tcaaaggatc atataaagga
    16741 agcagcgtga ccttcaatac acttgaacaa gatagttccg gacaaagcta ccaaatccgc
    16801 gcacaattcc gtaaatatcc ttcttacaag gaaagtttgg aagactatgc cgacttgatc
    16861 aaaaatggac tgactggcaa tcctgatttc tacaaaccaa cttggaagtc ggaaacaaaa
    16921 gactacaaag aagcaaccaa atatttggaa ggacgttatg ctacagatcg ccaatattca
    16981 caaaaattga acgcgatcat cgaagcgtac gacctgacaa aatatgatga accgaaaaaa
    17041 gaagcagcaa caactgaaga agcaaacgct gaaatcctca atatgtctaa gcgattcgat
    17101 gtgccaatca aatggacaac agatggcgtg ccaatcacag gattagaatt ttcaacgata
    17161 aaacgcgtca acaacgcttc ttccatcttc cgtgtcgctt caatctggga tttgtggaac
    17221 aattttacgg caagacagat accagaaaca actactcgta cagttaaagc tgcaactgtt
    17281 cctgtactaa ccttgttaag tttagacaaa tcattctggc ttgatctgcc tttaaagaaa
    17341 tagaataaat
//
LOCUS       DAGCWU010000049        17250 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.049, whole genome shotgun sequence.
ACCESSION   DAGCWU010000049 DAGCWU010000000
VERSION     DAGCWU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.049"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(31..576)
                     /locus_tag="MBZ71_001275"
     CDS             complement(31..576)
                     /locus_tag="MBZ71_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4429237.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(588..1280)
                     /locus_tag="MBZ71_001276"
     CDS             complement(588..1280)
                     /locus_tag="MBZ71_001276"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBT4429238.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1299..1583)
                     /locus_tag="MBZ71_001277"
     CDS             complement(1299..1583)
                     /locus_tag="MBZ71_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429239.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1596..2159)
                     /locus_tag="MBZ71_001278"
     CDS             complement(1596..2159)
                     /locus_tag="MBZ71_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBT4429240.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2387..3049
                     /locus_tag="MBZ71_001279"
     CDS             2387..3049
                     /locus_tag="MBZ71_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBT4429241.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3173..4369
                     /locus_tag="MBZ71_001280"
     CDS             3173..4369
                     /locus_tag="MBZ71_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBT4429242.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4470..5846)
                     /locus_tag="MBZ71_001281"
     CDS             complement(4470..5846)
                     /locus_tag="MBZ71_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4429243.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5883..6749)
                     /locus_tag="MBZ71_001282"
     CDS             complement(5883..6749)
                     /locus_tag="MBZ71_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBT4429244.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6838..7863)
                     /locus_tag="MBZ71_001283"
     CDS             complement(6838..7863)
                     /locus_tag="MBZ71_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4429245.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7881..8555)
                     /locus_tag="MBZ71_001284"
     CDS             complement(7881..8555)
                     /locus_tag="MBZ71_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4429246.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8739..9632)
                     /locus_tag="MBZ71_001285"
     CDS             complement(8739..9632)
                     /locus_tag="MBZ71_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4429247.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9706..11403)
                     /locus_tag="MBZ71_001286"
     CDS             complement(9706..11403)
                     /locus_tag="MBZ71_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBT4429248.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11396..12709)
                     /locus_tag="MBZ71_001287"
     CDS             complement(11396..12709)
                     /locus_tag="MBZ71_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBT4429249.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12709..16746)
                     /locus_tag="MBZ71_001288"
     CDS             complement(12709..16746)
                     /locus_tag="MBZ71_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBT4429250.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTAINRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16759..17217)
                     /locus_tag="MBZ71_001289"
     CDS             complement(16759..17217)
                     /locus_tag="MBZ71_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4429251.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4824 a         3883 c         3008 g         5535 t
ORIGIN      
        1 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
       61 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg aatctttgaa
      121 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
      181 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
      241 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
      301 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
      361 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
      421 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
      481 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
      541 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
      601 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
      661 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
      721 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
      781 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
      841 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
      901 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
      961 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
     1021 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
     1081 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
     1141 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
     1201 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
     1261 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
     1321 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
     1381 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
     1441 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
     1501 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
     1561 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
     1621 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
     1681 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
     1741 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
     1801 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
     1861 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
     1921 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
     1981 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
     2041 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
     2101 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
     2161 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
     2221 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
     2281 tctttagtcc gatactcttt ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa
     2341 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
     2401 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
     2461 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
     2521 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
     2581 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
     2641 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
     2701 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
     2761 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
     2821 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
     2881 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
     2941 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
     3001 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
     3061 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
     3121 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
     3181 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
     3241 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
     3301 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
     3361 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
     3421 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
     3481 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
     3541 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
     3601 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
     3661 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
     3721 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
     3781 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
     3841 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
     3901 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
     3961 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
     4021 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
     4081 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
     4141 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
     4201 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
     4261 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
     4321 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
     4381 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
     4441 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
     4501 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
     4561 acagtttgca gacgaagcat caatacaata caagtcaaga tggagacgat tggcaatagc
     4621 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
     4681 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
     4741 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
     4801 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
     4861 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
     4921 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
     4981 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
     5041 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
     5101 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
     5161 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
     5221 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
     5281 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
     5341 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
     5401 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
     5461 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
     5521 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
     5581 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
     5641 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
     5701 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
     5761 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
     5821 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
     5881 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
     5941 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
     6001 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
     6061 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
     6121 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
     6181 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
     6241 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
     6301 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
     6361 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
     6421 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
     6481 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
     6541 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
     6601 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
     6661 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
     6721 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
     6781 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
     6841 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc
     6901 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
     6961 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
     7021 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
     7081 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
     7141 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
     7201 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
     7261 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
     7321 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
     7381 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
     7441 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
     7501 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
     7561 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
     7621 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
     7681 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
     7741 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
     7801 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
     7861 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
     7921 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
     7981 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
     8041 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
     8101 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
     8161 tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg
     8221 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
     8281 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
     8341 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
     8401 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
     8461 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
     8521 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
     8581 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
     8641 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
     8701 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
     8761 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
     8821 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
     8881 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
     8941 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
     9001 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
     9061 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
     9121 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
     9181 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
     9241 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
     9301 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
     9361 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
     9421 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
     9481 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
     9541 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
     9601 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
     9661 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
     9721 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
     9781 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
     9841 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
     9901 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
     9961 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
    10021 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
    10081 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
    10141 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
    10201 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
    10261 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
    10321 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
    10381 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
    10441 aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
    10501 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
    10561 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
    10621 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
    10681 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
    10741 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
    10801 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
    10861 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
    10921 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
    10981 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
    11041 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
    11101 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
    11161 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
    11221 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
    11281 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
    11341 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
    11401 catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc
    11461 ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg
    11521 attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg
    11581 tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt
    11641 ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca
    11701 cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt
    11761 ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg
    11821 taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat
    11881 tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata tctaaataat
    11941 atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt
    12001 tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga
    12061 catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc gggtgataaa
    12121 ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt
    12181 ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag
    12241 gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta
    12301 ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata ttgtttctgt
    12361 tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt
    12421 catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct
    12481 gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat
    12541 ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt
    12601 ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg actgctgcaa
    12661 gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc
    12721 ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa
    12781 tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc
    12841 aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc
    12901 taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac
    12961 ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc
    13021 tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt
    13081 cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc
    13141 gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt
    13201 ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag
    13261 ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat
    13321 ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa
    13381 cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg
    13441 tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt
    13501 taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga tcccaggcaa
    13561 acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga
    13621 agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc
    13681 taattttcta actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca
    13741 taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg
    13801 cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc
    13861 atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt
    13921 ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac
    13981 tttctcacca tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct
    14041 ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat
    14101 ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt
    14161 actgccttca attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc
    14221 cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga
    14281 aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac
    14341 tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt
    14401 ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat aacgtggtgg
    14461 tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat ctcctaaaat
    14521 cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg
    14581 ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg
    14641 ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat
    14701 ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt
    14761 ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc ccgcatcaag
    14821 ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt
    14881 ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc
    14941 ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc
    15001 tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt
    15061 aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat
    15121 atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac
    15181 gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg
    15241 ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt
    15301 ctcttgatac ttatttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt
    15361 ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg
    15421 agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc
    15481 ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt
    15541 attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc
    15601 ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat
    15661 cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt
    15721 tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt
    15781 aagcggagtt cctccgattg gtgtacgatt tattgctgta ttctcacttc ccgtcaaacg
    15841 attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc
    15901 tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag
    15961 cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt
    16021 ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat
    16081 tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt
    16141 tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt
    16201 tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt catttcccca
    16261 aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt
    16321 agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat
    16381 caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt
    16441 ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcgggtagc gatgctgttc
    16501 gttttcaact tctccgctag gatagatata ttcatagggg tcttgcgtag ctgttggccc
    16561 aagacgctcc aaaataccgt tgaataactg gcgttgaatg cgctggagtg cttgaccgct
    16621 gggtacgatt ggttcgtcca gcttttccat ctcattttct tcgctacgtt gaatttcagc
    16681 ggaatcttct gtatcagttt cattttcttg taattcttgg ttatctattc tttcatcaac
    16741 caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg tttcaaaaat
    16801 caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt cttgaccatc
    16861 gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac ctgttccacg
    16921 aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac gtattctacc
    16981 ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt cttctttttc
    17041 aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat cctcctttgt
    17101 ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact ggaaatatgg
    17161 tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc ctttcacatc
    17221 cttatactcc tttcaaaaat atttgtcttc
//
LOCUS       DAGCWU010000050        16766 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.050, whole genome shotgun sequence.
ACCESSION   DAGCWU010000050 DAGCWU010000000
VERSION     DAGCWU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.050"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            131..373
                     /locus_tag="MBZ71_001290"
     CDS             131..373
                     /locus_tag="MBZ71_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBT4429252.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            391..693
                     /locus_tag="MBZ71_001291"
     CDS             391..693
                     /locus_tag="MBZ71_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429253.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            764..2818
                     /locus_tag="MBZ71_001292"
     CDS             764..2818
                     /locus_tag="MBZ71_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429254.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            2818..4650
                     /locus_tag="MBZ71_001293"
     CDS             2818..4650
                     /locus_tag="MBZ71_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBT4429255.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            5363..7261
                     /gene="ltrA"
                     /locus_tag="MBZ71_001294"
     CDS             5363..7261
                     /gene="ltrA"
                     /locus_tag="MBZ71_001294"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBT4429256.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            7378..8178
                     /locus_tag="MBZ71_001295"
                     /pseudo
     CDS             7378..8178
                     /locus_tag="MBZ71_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            8175..10706
                     /locus_tag="MBZ71_001296"
     CDS             8175..10706
                     /locus_tag="MBZ71_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429257.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            10756..11088
                     /locus_tag="MBZ71_001297"
     CDS             10756..11088
                     /locus_tag="MBZ71_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429258.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            11089..11682
                     /locus_tag="MBZ71_001298"
     CDS             11089..11682
                     /locus_tag="MBZ71_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429259.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            11845..13869
                     /locus_tag="MBZ71_001299"
     CDS             11845..13869
                     /locus_tag="MBZ71_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBT4429260.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            13869..15074
                     /locus_tag="MBZ71_001300"
     CDS             13869..15074
                     /locus_tag="MBZ71_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBT4429261.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            15090..15734
                     /locus_tag="MBZ71_001301"
     CDS             15090..15734
                     /locus_tag="MBZ71_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429262.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            15745..16551
                     /locus_tag="MBZ71_001302"
     CDS             15745..16551
                     /locus_tag="MBZ71_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429263.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            16573..>16766
                     /locus_tag="MBZ71_001303"
     CDS             16573..>16766
                     /locus_tag="MBZ71_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429264.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYV"
BASE COUNT         6209 a         2668 c         3481 g         4408 t
ORIGIN      
        1 ttccctatga gaaggatggc gtaaaaaaaa taatttttct tggatctagc aaggatgaga
       61 agcgaacgaa agagaaatta attcatgatg gatcacaatc aaataaatac aaattacaaa
      121 agactagcga gtgattgcta gtctttttat ttgtcctgaa ggaggaatca cagtggcaaa
      181 agctaaaacg ccatttgaaa aagccgcgtc taaaatctta gaacgccgcg gtgtatctga
      241 aacacagtgg aaacaagaag tccttgaaaa agcacaattt gacctattac ggggaaaaga
      301 caaggactta gaaggatata tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat
      361 tattaaacac taaaaggagc gaacaatttt atgtttgaat caattacaat gaatttttta
      421 acacaaccga aagtagtagc tgcggttgat atttttagag cattacaaac tgccgcaact
      481 agtttgaaaa ataatctgat gatttttgct gctgttgcgg cagtggcttc cattgctctt
      541 ggaggtattt tcttcttctt tgggcgaaga ggagctgaaa acgggaagac acacgtaggt
      601 tatgtattgt tgggggtagg agttatttta gctgccgctt ctattgtagt ttcaatgttc
      661 tcaatgtttg gacaaacccc acaaggacta taaaaaaaca agtagaagga gtaaaaagta
      721 cgtggctact ttttactcct ttttttatgg aaggagaata ttcatgtttt ttacgatttc
      781 aggcaatgca aaattggata aagagttaat aagaaaagca acgaagaaag tgggaaagcg
      841 tacttccttt ttaccgaaaa caattaaata ttctgtcatt gaaacggaag aacagctaca
      901 tgtgttagga caagtgttag aacaagagaa tcaatcttct gttaaattga ctctcacgtt
      961 acatgataaa gaaggaaaaa tagaaaaggc tggcacagaa tacgtttttg gctcactgga
     1021 ttatcaaatt tcagaaggtt tttatcctac attgattgat ttaatccgcc tagatatatt
     1081 taaagatacg gatgaaacca tgagtgtgga agagtcttat cgaaacgaaa aaattttaca
     1141 agttttagaa gaagagttag aaagcgacca aagaaaagtg aatcaagtga gtgaagtcga
     1201 agcaaacgaa gaagtaccat gggaacgccc aatctttcca aaggatgaat caaaagaaaa
     1261 agagaaagga attattgacg aacaagctga cattttgact cataaatcaa tatttgagga
     1321 agagaacgag gtaatgggtc aattggaggt agatcccttt gaagaaacaa tggaggatga
     1381 agagacggcc gaagtagaag ttgatccagt tgaagctaat gaagacccaa catggactga
     1441 aggggattct caggaatata ttgaaccact tatcagtgaa gaagtagaga aaaatgaaac
     1501 agagatacaa aaggcaacgg tatttgatta tattttggat caatatccgc cagagcatga
     1561 atggatgaaa gaaaaaaata aattattttt aaatgaactt tattcgaaat atcaattacc
     1621 tcaaacctat caagcatttt tagagagtaa agaagaatta atctcacagg gtaaaggcac
     1681 acttttagaa cagttagagt ctgtccaatc aacaaattgg catgagcttg cacaagaaga
     1741 tttattagct acttttgaag aacgtcaaca agaaacagat caagaaatta atgagtatgc
     1801 gacggaacag tcgtcgaatt gggagaaagc aaaagaggaa ctaaataaag aagaacagcg
     1861 agtgattgaa gaagaagtac aaaaaattaa agagcggtac gaaaagaaac gtgacaatgc
     1921 gtattccttg tgtgtagaaa agatcaacaa ttttactcag acgaatcaag aagcattatc
     1981 gaaagaaaaa gaaaacttat taaaagaacg tataaaagaa cgaaaagaag aattttaccg
     2041 agggttgcgt actgataagc taaggattag caaagaatta aatgaacaac tgaataaact
     2101 ctatcaaact acgactgaag ttctgatgga aaaacacgag cagatccaac aagaattaca
     2161 agcacaaatt ccaaaatgga aaaaagagta tgaaattcac caacaagaat tgaaagaaca
     2221 agaagaaaaa caacggaaaa aagaaaaaga gcgcgaacaa ttagaactaa aaaaacgtga
     2281 acaggatctt gcggaacaaa aaatggcgat agaaaaacaa cggctagaac aagaaaagga
     2341 aaaagaagca gctgctcgta aggaacgtca agaacaaatg gatacattgc gacaagcaca
     2401 gttaaattat ctgacacatc ctcaaccagt agctgctgtt caaacgccag tgaacaacca
     2461 agcaaattta actccttcac cccagcgttc ctttaacgga tggatggtgg gatgtattgc
     2521 aagtttaagc ttgcttctag gtggaggaac agtatttgct tttaatcata tcaatcaatc
     2581 ttcacaggct gattctgtag ccacaacggc gtcaatcaag gaagaagccc aggaacaagc
     2641 aagaaaagag tatgaacaac aattagaaga attattgaat cgtaccaatc agtcaacaac
     2701 agagtcaagc atggaaaata gcaaagagag tgattctagt aaggctaaag aaggatcaag
     2761 cgattcaact gtacaatcaa acacttccga aagttcagaa tcaaatgagg tgaaataatg
     2821 cgtgttagag aaacaacgaa gcgaaaagac aaacgattca atttagaaaa gaataggtat
     2881 ctttctcatt tctatcgcta tactccctca ggagaagagc taccagaaaa acatgacaaa
     2941 cgcatttcga aaaagatttt ttatagtgga cttgtatacc tccctttaga gtatttagta
     3001 acaagagtac cagaagtttt caaccgattg gaaattgtta agggaagctt acaaggaata
     3061 aatctgtttc aattacagga aatgccacag gtctttcatt ttgattcagc tggtttttgg
     3121 aatccattta ctcctacaac tagtttgggt acacgtttag caatttcgac agtttgtgtg
     3181 gttccagctg tacttatggg attgaaaatg gacatgtggt ttcgtcctct agcagatgga
     3241 caaaaagggg ataatcgatt aatgacctta aaagaaatcc aacagattta tccgcaaata
     3301 cctgaaacaa aaactacttt tccaggttat ggaggtattc caatcacaca ttacaaaaaa
     3361 tattggtaca ttcaaacaga tactgttaat acgtgtattg ttggtacctc tcgatcaggg
     3421 aaaggtcaga ttgaagtgtt agccaccatt gacaatttat ctcgtgcaga aaaacaaagt
     3481 tcaatggtcg ttaatgatcc aaaaacagaa ttatatgttg gtagtaaaga tgaactagaa
     3541 gctagagggt atgatgtata tgcctttaat atcaccgatc cactgcaatc aatgagtgat
     3601 aatccattgg cactcatcgt gaaatactgg aaacgaggag acatagatac tgcaacacag
     3661 ttaaccaata ctttcacaaa tacgatttat tatgatgcca acgcttcaga taacaaatac
     3721 tttaacgaaa acgcgcagaa agctgtcaat gcgcttattt ttgcgttgtt agaacaagct
     3781 gatcaaacag gagattactc caaattgaca ccgaataatc tcgttgagct tctttctgag
     3841 attggcggtt ttaattatta tccagatcca gacaatgaat tcaaacagtt gaatgcctta
     3901 gatgaattta tgaatcagtt gccacctgga aacattgcaa aaaaacaata tggggcaacg
     3961 aaaattggat ctgataaagc gaagggaaat attttatcaa cggcaatcac gggattgaat
     4021 ccatttactc taacgaaaat tgcaaagatg acttctcaat caacgattga ttataaatcg
     4081 gttgggtttc caaaatactt agatttgaaa ttacatgaaa gtcttttaaa tcaacgagtc
     4141 gtagtagaat tttatcataa gggaaagaaa tttcatgaag aacgtgtcaa agtaggctat
     4201 aaaggattta gtgaaatcaa ttttgattgt catttagttg caggtgatca agtttgttta
     4261 aaatacgatc atcaaggaaa agaatatgtg gcttggtatg aattttctca aagagtattg
     4321 aaagacgaag agggtaatat cgtttatcgg aaaaaacgag gagaaaccca tttgccagaa
     4381 ttagaaaaag aagccgttct aacattagat gaggaaaaaa gtaacttatt agtagagtct
     4441 attcggatgc actataccga taaaccaatg gctattttta tgcttacacc tgattatgat
     4501 ccatctaacc atatcattgt tagtattttt cttagtcagt tatataaaga attatctacg
     4561 caatgtgtta agactaaagg agataaatgt caccgcagga ttcattttat tttagatgaa
     4621 tttgggaata gtgtgacctg tttagcctaa ggataaaaga ggctagttcc aacgaacatt
     4681 ggaataactt tactatcgag aaatcaaatg acggggtaac gccctgaagg gtttccactg
     4741 atactgcgac atgcgttagt aggatgtgca tccaaacgcg tatgaagctc gctgaagtcg
     4801 gcagaagggt accgtaagga aagacacgat acgtatctac cacgcaagtg ctctagaggt
     4861 agggtatgta cctgatatgt cgggtgtcct aaaaagttca ataaggtgag aatgatttga
     4921 ttatctgaca aacttgcgaa tggaatgtct gaaacgaata actcatagaa atgtgagggg
     4981 gaagggggat acgtcctctt tccattgact cgaggttgag taaaaatgtg tttctatgaa
     5041 aaaccttgca tggttaaagg ctatggagta gtcaacaggc aaaagcaagc acctaagttg
     5101 aaatctatgg atagatagta aggaacaggg gaagcatcta gcacggaggg agcataccga
     5161 agatgtgttt ctaaggaaaa accgaaatcg gttataactt aggtgaatag atgtgaaagt
     5221 agagccacga ccagtgaaaa tttcgtaatg aaattggagg aacaggctca agtcgttatt
     5281 tataatataa gaaacctatt taaaatcatg gattctcgta agactaaaaa ggtcacgaac
     5341 cagaatggag cgtgataaaa ccatgactaa cacaaaagga attagcttaa agaacaaaca
     5401 actcagacac gccgaatact acgacatgac tgagactttc gatgaattgc acgcaatgag
     5461 tgttgcaaaa tcaaatttta aaaatctcat gaaattcatt attgatgaca ataatatctt
     5521 tctagcgtat agaaatatca aaaagaataa aggaagtgca acggccggat acgatggtgt
     5581 cactataaaa gacattgaaa aattggcatg tggtgacttt ttaactatta ttaaaaggcg
     5641 atttaaggat tacagaccaa gaaaagttaa aagagtagag atcccgaaac caaacggaaa
     5701 acttcgacct ttaggaattc cttcaatatg ggacaggata gtccagcaat gtattctaca
     5761 aattttagaa ccaatatgcg aagcaaaatt taataagcat agctatggat tcagaccaaa
     5821 ttgttctaca gaaaacgcca tagcagattg tcttgctaga atcaatcaaa gtaagatgaa
     5881 ttatgttgta gatattgata taaaaggatt ctttgacgaa gtaaaccata ccaaacttat
     5941 gcgtcaaata tggacaatgg ggattcgaga taaacaactc cttgtaatta ttcgtaagat
     6001 gttaaaggct ccgattgtta tgcgaaatgg caatgttgtt tttccaacca agggtactcc
     6061 acaaggaggg attttaagtc cactgcttgc gaatatatgc ctgaatgaat ttgattggtg
     6121 gatagctaat caatgggagg aacgaactgt caaagagctg aaacaccact atcgaaaaaa
     6181 cggagcatgg gataaaggaa atatctattc gaaattaaga agccacacaa gtttaaaaga
     6241 gatgtatctg gtacgctatg cagatgattt taaaattttc tgccgaacag gagaagaagc
     6301 ccaaagggct ttttatgcca cacaaaaatg gctcagagaa agattaaagc tcccaatttc
     6361 aatggagaaa tctaaagtta cgaacctaaa taaagctgaa agtgaattct tgggtttttc
     6421 aataagactc cacaaaaagg gaaaaaagaa agtggctttc acgtacgttt gtcgcaaagc
     6481 agaagaaaac attaaaaata aactaaaaca acagattaaa aaaattcaat acgctggagc
     6541 aaaaaaaaat aggcaccgag caataggtga ctacaacatg atggtcatcg gtatccacaa
     6601 ttactatcgg atagcttcac attgtagtgt caatttcact gagatttata gaagtgttag
     6661 gacagtaata gaagcaagac tgaaagctag aggaatgacg aaaaatggaa tctatggaca
     6721 agatcatgaa ggataccgtc cgtatctaaa gtctaagcaa atgagatata ttgaaggttt
     6781 tccgatactt ccaattggct atattaaaaa aagaactcca ctcaacaagc ggagaaatct
     6841 aaataaatat accaaggaag ggagaaagct cattcatcaa gagcaacaag ctatagaaag
     6901 ctggaaaatc aagtatcttg taaaaggttt tagtaacaat aagaaataca cggtggaatt
     6961 ttctgataac aaaatatcta agtttatttc ccaaaaaggg aaatgcgcta tcacaggaaa
     7021 cgaacttttg atggaagaaa ttgaatgcca tcacattaac ccatatcatt tgtccaaaga
     7081 cgatagttac aagaaccttg tcattatctc tagagaaatc catagattaa tccatgcaac
     7141 tagtgaagaa actattctga aactgcttcg gttactgaaa ctaaatcact atcaaattag
     7201 aaaactaaac acgtatcgaa aaaaagcagc taattttcca atatcaatca aaatgaatta
     7261 gataggaaac aaattatatt gtaattaacg atggaacgcc gtgtgcggtg aaagtcgcac
     7321 gcacggtgtg gaccggggga aaagacggag ataatatcaa actcttacct atcggaattg
     7381 ccaccaatgg ataatatgga aggaatcatg acggtcacag ctggccgaaa catgttgtgg
     7441 gatctgttta ttcaatctta tcagcaactt tatgcgaagt atggagaaca aaacgcagcg
     7501 attatcaaag aaaattgtca gacacatatt tatattatgt cgacagatga taataccatc
     7561 gaagaatttt ctaaaaaagt ggggaataag acggttgaac aagagaacag taccatcaat
     7621 tcattaggta tgaatactca tatcaatcgc aatgttgatt cagatcgtat cttaactcct
     7681 gaaagaattt ctactttttt agaaggtgaa acgattgtac tcgctccttt aaaacgacaa
     7741 gatttgaaag gtcataaaat acggccgtat cctattttta atcatgggga aacgaatctt
     7801 ccttatgctt atcaatatat tgaagatttc aatccatcgt gggatatcaa tgatatagac
     7861 atacattgta cacatgcgga cttggatctt aaggctttac aaattgatta tgattatttc
     7921 ctaccagatg aatatgctag aaatctctat catggactgc atgatcaatt agaagaaaaa
     7981 aacgaagaaa atatgtttta taaaatcgtt caatctcttg agaatgagac ggctaaaaaa
     8041 ttgttaattc aattttacga agaacaagca gcagatcgac ttcaagaata tgttgaaaag
     8101 ttgatcaaaa atgaagaaat ttctgaaatg attgggaaac ggttgttatt ggaatctgaa
     8161 aaggtggtga aagaatgatc attggaggaa ttttttctgg catagccgac tggtggtcag
     8221 gcttattcgg agaaggggat gcaaacccca aaaccgtaac gaaagtctta caagattatg
     8281 gtaattatct gcagtatcaa gatgtgttca gttggatttg taatactttt ttatgggggt
     8341 tgatcaaagg gttatataat ctcaatactt tactagaaaa aaccatttat caaagctttg
     8401 atttaaaaga cttattgaac gccgctgggg taaatgaact atttcaaagt ttgattagta
     8461 aggtattggc tcttttttgt gtcgtcacat taatttattt aggcttgaaa ttttttatga
     8521 gtaaacaccc accaaaagtc aaaaatatct tggttaattt atttatggcc atgatcttga
     8581 tttttggtgg tagttcttta atcgatgaag ggttaaatat cagtaaaagc ttttacggag
     8641 acgtcactgc agcaaataaa gcagataaaa cgggttcacc tgcttttcaa attattcaaa
     8701 ataatgtgat cgatgtcagt acattactag caactgaccc gaataaagta gaatccctgc
     8761 caacagacaa aagaaatgct ttaacagcga aaaattttaa aacagcgaat atcaatggga
     8821 ttattactcc taaacaagct aaagaaatgg cggataaaga taagagtggt ttatcagatt
     8881 tacaaaagga tcggttaaga gcgttacaat accgactgga actggatgat aatggaaaag
     8941 aaattcctgt gcaaatcagt gatgaaggct tagccaaata tgtttatact tcagggtatc
     9001 gtcgatacat tagtagtcca gggattattt tagctggtga atcttctttg gcggtggcct
     9061 atttatttat cctatttacg atcgttactt gcattattga gctggtattt aaaaaattct
     9121 atttagtgat tgccgcgtca acggactttg agacaggaca acggatgaaa agtgctatcg
     9181 aagatatttc tcaatccttc ttgttattgg catttacaat tttagaatta cgaatctata
     9241 ccttaatgct ttcaggtatt ggtgatttgc atgcggcagg aaagatcaat ggattcttgt
     9301 atgttgtcgc tttgattgta ctaaccattg cgctattcaa aggctctcaa tcagttacga
     9361 aaattttcgg cgttgatacg agtctgaaaa acggcagtaa ttctcttctt agcatgtttg
     9421 ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa aaaccttgca agtgctggta
     9481 agagtggttt aagccattta aatgatattc gtaaaaatgg atttggccat cgaaaagatg
     9541 ttacccctga taacggggaa gaacaatcag acgataaagc tacaactgta cctaatagta
     9601 agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg tgtttctaaa gccgcagaag
     9661 gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc tgaagatctt acagaaaaag
     9721 ccaaagacac cttagaagat aaattagacg gcacgagagc taaagaattg gctgaagctg
     9781 tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa agcttcagaa gtcaaagaag
     9841 gactaaaaga tgcgtatggt gaaggacaag tcaatgcagc agtgaaagct aatacaacaa
     9901 ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt caaagatcac caagaagcgt
     9961 tgcactctaa tgaaaattcg attgaagaga aaaattccat agaagaaaga aattcagaag
    10021 aaggaaaaaa tggacagtta ccaaaaactg atccagaaca atatcgctat aaacctaaca
    10081 gtgaccttga aaatgagaag cgaatttcag atttatcaaa agtaaatcaa gaagacaaag
    10141 cagaaaatga gggacagtta ccaaaaactg atccagaaca atatcggtat agatctgtta
    10201 actccgaaaa tgaaaaacgc atttcagatt taccaaaacg aaatcaagaa gtacaaacgg
    10261 atgaggattc catcgttctg ccaaatgata atggaatcga agttggaact gaaaaatcaa
    10321 cgaaaattgg tttacctaag gcacaaaatt taccaatgaa agatagtcgt ttgaatcaat
    10381 tcaaggataa tgatcaaagt tttgttcatc aatcacctgc aggtactgtt caaatgaaac
    10441 caagtcgttt gcctgaaaat ggaaatctga caactgtttc ttacaattta tctgaaagtg
    10501 gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag agaagctgca ccaaaaccta
    10561 ttcaaaacgt tcatacaggt tacaagccag caaatgtggc atttactgca ccaacaaaaa
    10621 taaatgtgac aagagaaact aaaaacagga acaatgcatt gaaagaacaa ctattgaggg
    10681 atatcaaaca ggatatcaaa ccataaaagg cctacaagtt aggccttttc tttatatgaa
    10741 aaggagaaat caattatgga taaatatata aacatgaaaa accctccaag atttagtgga
    10801 gaatggaagt ttttcgcttt tttcttttta gatctattaa cgcttggtgg ctgctttttt
    10861 tgggcgaaaa tggtcaataa tcttgtccac ttaccactct tttttgcact cttgaattat
    10921 ggcttatgga ttgcgatagc gttagtgatt gtttggcgac cctataataa cccaggtcaa
    10981 cgacaaattt ttgcgattat cgagtctatt ttttttgcgg acaagaacca ttatcatgcg
    11041 ttagatccta accgaaatta tcatggtacg tataaggagg atgaataaat gcgagtcaaa
    11101 ggacaaaatt cagttgaaag gaaatccatt ttgcagtttg gaaaaccaga agagcgctct
    11161 atcttggact tatttccgta taaatcctgt acagaagagg gagtcttaat tactagagaa
    11221 gatcattttc aacgttatta tcgtgtagtt tctacagacg tggaaggatt aaatgaacaa
    11281 gagaaactag aacgaatgaa tcagttgacc accgtcatgc gtacgtatgt gccaacaatt
    11341 aagttagtta gtttgaccac agagacggat ctaacggaac aaatcgtgca aaagcggcag
    11401 ttattagata aaaatcgtat ggaacagtcc ttaggaagaa atctacagcg attaagaaaa
    11461 tatgaaaaga aattagtaga ggaaatccag gaattgaaac gagcagaaca ggaacgccct
    11521 gatttaagct tctttttcgt cattgaagga aagacaatca aggatttgca aaccagaaac
    11581 cgtcagttaa tgcgatctag tggcatttta gggttaaaac cactctacaa aaaagaattg
    11641 atcaagattc tttatcgaat gaataatatg aatgatgagt aggaatgggg aagagagaat
    11701 gaaatatccg ttgatatggt tagaaaaaga tgttccgaaa gaagagacca ctagaaaatt
    11761 tcctcacact ttaagcctgg gtaagttggg aaaaggaaaa tcttttaacc aagtaaagaa
    11821 tcaaacaaga agaggtgact gtacatgtta gaagcaatta aaacagcttt ttcaccacaa
    11881 aatgatcaaa aggtacttga tccattaaag caactaggaa atcgggaaat agaaatttta
    11941 aaatccaaag ggtatgattt agacttttta aaacaagtga tgccacaagg tggattaaat
    12001 ttcgatgaag agtatgtgac ctatggaaat ggttattcac gatgtgtgac agtttatcac
    12061 tacacaaaaa atcctacgct tttttggttg gccaatctca tgaacaatcc tcatacagtg
    12121 gcaacactag atgttagcac agatgaaaag caaaaagtga ttgatcaaat caatcgtgcc
    12181 ttagatgaat taaatgatcg gtcagataat gaacgaaaag cgactgaccg aaataaaagc
    12241 gcggaagaat acctagaact agcggaatat gcccgaaaat tgacttctaa tggggaaatt
    12301 tctaaacaag tcaaagtgag agtacatgtt tacggaaaca cgattcaaga agtagatgaa
    12361 cgagcacagg aactgatcga aaatctcaaa gggatggatt atcgtgcagt gacctatctg
    12421 cacaaaacga aaagagactg gcaatcgatg ttcaccatgt tcgatgaaca agctgaatgg
    12481 tttggaaata tcaaagggca atcactacca tcgctaaaca ttggcggagg tttcccgttc
    12541 caccaccaaa cgctaaaaga tccaggtgga acacaatatg gggaaaccat gacaggcggc
    12601 ccgtttgttt ggaatgccac gtataaaaat agtgtgcggt tgtctaccaa taccttgctt
    12661 ctaggaatga tgggagctgg taaatcgacc gttcttaaaa tgatcgcgga agcccattta
    12721 gctgcaggag actttatttg gggctttgaa aagggaaaag actttattcc ctttttaaag
    12781 gaatataacg gcattatggt gcgtttagac ggatctgacg ggatgatcaa tcctttagaa
    12841 atatttgcca cacgtacgta tgatgaagct tccagtttat atgacgatgg atcagtcaaa
    12901 gatttgaaga tcaatgaagc agcgtcctat caaacgcact tagacaaagt ggtctatcag
    12961 gtacagttag tgagtcctca gctaaaagga acgatgaaag cagaatttaa gacctattta
    13021 aatcgttttt atgaagagta tggaacagta ccacgcggct ttacttcctc aaattcgcgt
    13081 tcaaatactg atacgcaagt cacaggtaaa gatccagaag cttatccgac gttcaaagaa
    13141 tttttagact ttttaggaca actggaatta ccaggtgctt cccaagagaa aaagaatcga
    13201 aaagaagaaa tggaatccat cgtagaatcc ttatgtgaga cgtatggcat gatttttgat
    13261 ggtcatagta cgattcgaca ccttgaccaa caacagttgg tcttttttga cattgactct
    13321 attacaggct tagatcgagg ggtacaacaa tgtcaaatgt acatggctat gacgttgatt
    13381 tggaatcagg cattgattca aggaagaaga caacgacgtt taattaaaga aaaacaaatg
    13441 gaagaacaag acttcaaatg cttcttggcg atgattgatg agtgccataa tgcgattaat
    13501 ccagatttta ttgaaacggt aaattatgtc acaaactttc aacgagaaat gcgtaaagta
    13561 gaagcaatga ctgttttagc tacacaatcc ccacaagaaa tggtgccaga aaatatgtct
    13621 tctgataact tctcgaaact gaagaaagtc tttgaatttt cttctacgaa gatctttatg
    13681 cgtatggatg aatctatttt acgtcatata gaaggattag ttggtaaatc aatgacaaac
    13741 tcagaattag aatccattcc tcaacaaaat caaggagacg ctgtgatcaa ttttggctca
    13801 aaagaaacca ttcgtgttca ttttagtccg aacaaacgtc aattaaaatt gtttgatgga
    13861 ggaaaataat ggaaaaccaa aatgaaagtt taatcaagca gtatgttaag cgtcgtgcaa
    13921 agcgacgctt atttttgtgg ttgtttggta cttctgcagg tttgattact attttgatta
    13981 ctgtgttcgt gacccttttt ctaatacttg cggcgggttc gattgataat tctgattcag
    14041 attcaagtag tggtggcgaa gcttttacag gagaatattc ggaaggctta ccgatttata
    14101 aagaaatcaa aggaagaggc cctttttctg atgagattgc gcaatatgct gtaggtgcag
    14161 cagtgaaata taaattatta ccaagtgtga tcctttcgca atacggttat gaatcagcgt
    14221 ttggtacgtc ggcgtccgca agaaatgatc tcaactattt tgggattact tggtttgatg
    14281 gttgtctgtt tcctaaagga actgcgcgag gaatcggtgg tattgaaggt ggctggtaca
    14341 tgaagtttcc caactcaaaa gcagccttct cctattacgg ctttatggta gccacacaat
    14401 ccaattttaa tgcatgtgta ggaaataaaa gccctggcgc aagcttattg atcttaggac
    14461 gaggtggtta tgccgctgca ggaattactg aagatagtcc gtattataca ggttgtatgt
    14521 ccattatcac ttctaataaa ttaaccgaat atgatgaatt cgccatcaaa cattggggtg
    14581 aaggtggcaa taacaacggc acgatcactg gtgaatggac gaatccattc cctggcagca
    14641 gtttagataa aagttccttc agtggtggtc aattatttgg aacaaatcca ggcggtgaat
    14701 tccgtccgaa tggcttccat gatggattag actttggttc agtcgatcat ccaggaagtg
    14761 aaattcacgc ggtgcatgga ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt
    14821 taggtgcatg tgtgatcgtg attaattacg acggtttgaa tatggtatat caagaatttg
    14881 cgaatagtac aggaaattct cgtgtgaagg ttggcgatca agtgaaagtt ggtcaagtaa
    14941 tcgggatacg agatacagca cacttgcatt taggtttcac tcgaatggat tggcgtcaag
    15001 cacaaggaca cgcattcaca gacgatggta catggattga tccattacca tctttgaata
    15061 gcagcaaaaa atagatcagg agaaaaaata tggaaaataa aactttaaaa atcgtcttaa
    15121 cagctgcaat tagcgttagt ttaatcggca atatcgcgtt gttttggcag ctgaaagaag
    15181 aacaagagac aaataccttt aaaattgaag aagtggctaa aaacgcgtct gagaagctca
    15241 aaaaaaattc ctctgaggca caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga
    15301 aaaaccaaga aatcgatcag ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa
    15361 ctgaagcaca aagaaagacg gcagaagagt tcggtcaatt ggccattgat aaatcattaa
    15421 gtagaaatga attaacggaa aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat
    15481 taagtcctgc agacgatgat caccaacatg atgattacgg aagttactcc tttacattag
    15541 gtgacgtgca aacttatgct caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat
    15601 ttgttgatta tacaatcggc aatccgcaat tcaaagatgt caaaccacaa aaaataaaag
    15661 gtggattaac tgtcactgaa aaacaagtag atggtcaatg gaaagtcata gactttagtt
    15721 atttcactcg ttaggaggaa gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa
    15781 aaacgaaaaa gttatcttca atggctggga atctttatga ttctcggctt tttgattctt
    15841 gctagtccga tttatacgaa tcagaaggta gatataccag atgaggtaag cacgaacgcc
    15901 tttttaaaca cgaaagagct gacaatggtt tcaaaagaaa aaggagcgaa ccagcaatta
    15961 gtttttgaat ttgcgatcga tgatgaagat caaagcttgt taaaagaact ggctaatctt
    16021 aattatcgcg tagaagtaaa gacagctaaa ggggattatc aagcaatacg agccaaagtg
    16081 atcaaggtgt ctgatgatta ttttgcagtg aagctaacgc atgtaccaga agaatatatc
    16141 gccatgcgtt taaccattat tcctgaaaaa attgatccaa aagtggatat gcaagaacca
    16201 caagatctga tttattacgt gcacgaagat aaggttaaag atcaagtgaa agatgaagat
    16261 tatgaacaac acgcgatcaa ttacaaagtg aaaggataca agaaagaaca aaaagcagct
    16321 caaaaacaaa tggaatcttt ccaagcaacc attgatttga atgaagaatt agtgaagaag
    16381 ctaaaagatc aattgcctta tcaagtcgca gatgatcagg aaaatactga aaacaagatt
    16441 aatagctacc agcaagaaat tgaaacatcg aaaacgcaac tgaaagatca agaagagatc
    16501 attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag aaaagattta gaaggacagg
    16561 tggagtgaaa aaatgaagcg aacaaacttg gctgacaaag atactttcgc tgagcagaaa
    16621 gtattgatta aagcctatct caaagttgta aaagctaaag aaatatgtcg agaagtgaat
    16681 ttatctaaca caacaatatc tgagttgaag aatggtcgaa agaatttaga tactttaaaa
    16741 ttcagatcat ttgaagctct atatgt
//
LOCUS       DAGCWU010000051        16596 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.051, whole genome shotgun sequence.
ACCESSION   DAGCWU010000051 DAGCWU010000000
VERSION     DAGCWU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.051"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..130)
                     /locus_tag="MBZ71_001304"
     CDS             complement(<1..130)
                     /locus_tag="MBZ71_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBT4429265.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKY"
     gene            complement(132..329)
                     /locus_tag="MBZ71_001305"
     CDS             complement(132..329)
                     /locus_tag="MBZ71_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBT4429266.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(723..2450)
                     /locus_tag="MBZ71_001306"
     CDS             complement(723..2450)
                     /locus_tag="MBZ71_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBT4429267.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2443..3636)
                     /locus_tag="MBZ71_001307"
     CDS             complement(2443..3636)
                     /locus_tag="MBZ71_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4429268.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3823..4467)
                     /locus_tag="MBZ71_001308"
     CDS             complement(3823..4467)
                     /locus_tag="MBZ71_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4429269.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4561..4695)
                     /locus_tag="MBZ71_001309"
     CDS             complement(4561..4695)
                     /locus_tag="MBZ71_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBT4429270.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4697..6829)
                     /gene="feoB"
                     /locus_tag="MBZ71_001310"
     CDS             complement(4697..6829)
                     /gene="feoB"
                     /locus_tag="MBZ71_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBT4429271.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6826..7299)
                     /locus_tag="MBZ71_001311"
     CDS             complement(6826..7299)
                     /locus_tag="MBZ71_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBT4429272.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7700..7924
                     /gene="nrdH"
                     /locus_tag="MBZ71_001312"
     CDS             7700..7924
                     /gene="nrdH"
                     /locus_tag="MBZ71_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBT4429273.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8083..10242
                     /gene="nrdE"
                     /locus_tag="MBZ71_001313"
     CDS             8083..10242
                     /gene="nrdE"
                     /locus_tag="MBZ71_001313"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBT4429274.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10292..11257
                     /gene="nrdF"
                     /locus_tag="MBZ71_001314"
     CDS             10292..11257
                     /gene="nrdF"
                     /locus_tag="MBZ71_001314"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBT4429275.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11346..12485)
                     /locus_tag="MBZ71_001315"
     CDS             complement(11346..12485)
                     /locus_tag="MBZ71_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4429276.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12794..13507)
                     /locus_tag="MBZ71_001316"
     CDS             complement(12794..13507)
                     /locus_tag="MBZ71_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBT4429277.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13761..14462)
                     /gene="nagB"
                     /locus_tag="MBZ71_001317"
     CDS             complement(13761..14462)
                     /gene="nagB"
                     /locus_tag="MBZ71_001317"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBT4429278.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14696..16228
                     /locus_tag="MBZ71_001318"
     CDS             14696..16228
                     /locus_tag="MBZ71_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4429279.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16419..>16596)
                     /locus_tag="MBZ71_001319"
     CDS             complement(16419..>16596)
                     /locus_tag="MBZ71_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4429280.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5149 a         3421 c         2870 g         5156 t
ORIGIN      
        1 catatttttg tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat
       61 atttaggatc tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat
      121 tttttttcat tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt
      181 cctgcaatac aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac
      241 actccatttc cataatattt tccactatga gtggtacccc catagataag ttgtgtctct
      301 ttaatagaca aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag
      361 actattattc taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa
      421 taattcctga ataagaattt aatcactttt aagtatataa actatgtata ttctttaata
      481 ttttttgaga atgcacttcg tacataataa actttaatca cattagtctt tcatacctac
      541 gatcgatctc tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttgaacgt
      601 ttcgaagatg agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg
      661 agcttccaaa tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct
      721 ttttattttt tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct
      781 tgctgtggat cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac
      841 caatggaaat tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga
      901 accatcgtat ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc
      961 agatcatatt tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt
     1021 tttttgatcg cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc
     1081 cagtctttca agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct
     1141 cctggctcga ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt
     1201 acatccatgt aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg
     1261 tcttcgactt gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct
     1321 gaaaccatgg catctgactc gccatttgaa acagatttcc acatgattga attatctaat
     1381 ggcgtcatcg ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa
     1441 gcaacttctg tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat
     1501 gaaaaactcc ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt
     1561 ccttgacgtt ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg
     1621 ttcaaatgct gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg
     1681 acaaatccat tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg
     1741 ggtgcaccga tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg
     1801 atccctgcca tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga
     1861 cttgtgctcc cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct
     1921 aaattcgtaa aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga
     1981 accatcccga taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc
     2041 tgcataaagt caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag
     2101 atccctaatg gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca
     2161 gtattcatca aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg
     2221 aacaacgtaa gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag
     2281 ataaacaaag gagctggaat cactaacagt aaatttgtaa ttcccgacat cactgcctgt
     2341 ccaatcgttt gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata
     2401 ttttctaccc agtttgccac tggtaattga tagttcaata gattattcat gttcactcac
     2461 ctcgcttgtt tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt
     2521 tccatcctct acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact
     2581 taccaccatg tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg
     2641 tttttgcttc ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt
     2701 tctgtcaaca gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt
     2761 tggtccatca gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag
     2821 cactttggaa cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt
     2881 caatatttct tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct
     2941 gtccccaatc cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac
     3001 attggcttgc aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc
     3061 cgagaacgct tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc
     3121 aacccgttgt tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa
     3181 actagaattt tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg
     3241 gacttctaac ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt
     3301 ttggaaaacc atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt
     3361 cgatacatct tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa
     3421 tagacggatc aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc
     3481 ttcttttacg tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat
     3541 ctccgttttc ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa
     3601 tatctttgtt aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa
     3661 ttcatttttc tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc
     3721 aaaaatgaaa gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata
     3781 aaaggtttag ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg
     3841 ctccgggtaa aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac
     3901 gatgtcccct gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg
     3961 agaaattgcg ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg
     4021 gtccagatgc attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt
     4081 atgtattcgc ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc
     4141 atcttgttgc ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac
     4201 atcttgatat tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact
     4261 tccatgccgt acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt
     4321 ctcaggagaa gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac
     4381 tttatatcgg ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg
     4441 gcggacgatt tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca
     4501 tcttactatg ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga
     4561 tcatcgcttg atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt
     4621 accggatttt aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa
     4681 taagaatgtc gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct
     4741 tggattttgg ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat
     4801 acgtccctaa cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac
     4861 taactaaata agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat
     4921 tcatttcccg atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg
     4981 ctaacaatga ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc
     5041 tgccattttc ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt
     5101 ctttagcgac caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg
     5161 gctgaaaaac aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa
     5221 caggttgagc ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga
     5281 taatcgttcc tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg
     5341 ctgaccattg aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag
     5401 caaacaaacg tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa
     5461 aataggcaga aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa
     5521 taatcggcaa ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat
     5581 cttgctcgtt ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa
     5641 tcagcatagg aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga
     5701 caaaagcgat cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata
     5761 taatcaattg cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga
     5821 tcagctcctg catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg
     5881 gtaaccatgt accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt
     5941 gaatagccag ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac
     6001 gattcgttgc aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca
     6061 caacacagag tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct
     6121 cactatcgtc gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct
     6181 gagaaactgc tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg
     6241 cataccagcg tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg
     6301 cttctaaacg tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact
     6361 cctgaatctt ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa
     6421 ctggtacacc aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga
     6481 tgacatccat catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag
     6541 ttaaataaag atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct
     6601 gttcctgaac caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat
     6661 agatgccggg taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt
     6721 tttctactgt tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga
     6781 acaagctagt tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc
     6841 cctttgtatc aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac
     6901 tgtcagttcg attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg
     6961 cgtcaatccc atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac
     7021 gatcccagtc tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt
     7081 ctttttattg acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt
     7141 taccagtaaa atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac
     7201 gatcccgata ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg
     7261 ataaacgaaa ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa
     7321 taaatcatgt attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata
     7381 aatgagaatc attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg
     7441 atattatgag cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata
     7501 gttttccaca attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag
     7561 ctcttttttc aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg
     7621 tttgttgttt gactttttta ctaagagttt tatactatct attatagata ataagaatgt
     7681 gttagaggag gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg
     7741 taagatggtc aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga
     7801 tgaacagcct gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat
     7861 tacttctgat gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag
     7921 ctaacatgac taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc
     7981 agtttttgtc gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc
     8041 aatcaagcat catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta
     8101 caagatgtca gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc
     8161 cctttgaata aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat
     8221 acgatgactt ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata
     8281 gaagcagaat tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg
     8341 gacgagcaac atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat
     8401 gcgttgaaaa ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc
     8461 aatgcgcttt actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg
     8521 atccatcaac gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg
     8581 ggcgaattgg tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc
     8641 tcaatcaact ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca
     8701 aatcttcgtg aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt
     8761 ccagtcatga aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa
     8821 ggagctggcg tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca
     8881 aaaaaagaaa atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta
     8941 cctgataaat tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat
     9001 agtgtcgaaa aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat
     9061 catctagttg caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat
     9121 gaaatctcaa aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg
     9181 aaccgcgaga atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta
     9241 caagttcaaa cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat
     9301 atctcatgta acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt
     9361 caatctgtgc gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat
     9421 gtcgtaccac cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg
     9481 ggcttacaca cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac
     9541 tttacaaata tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc
     9601 gctcgtgaaa gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact
     9661 tatttcgaaa aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg
     9721 tttgaaggca tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg
     9781 aaagatggac tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat
     9841 atcaacgata cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag
     9901 aagaaaatcg gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat
     9961 tacacttctg cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa
    10021 caacacgtcg atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc
    10081 atttatgaat ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat
    10141 tacgctttcc acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca
    10201 gaagaaatcg gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc
    10261 ttattcatat tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa
    10321 ctggaatgaa attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt
    10381 ttggctagat acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca
    10441 attggaaaaa gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca
    10501 atctgaaagt ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt
    10561 tttgaacaat attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag
    10621 tacactgaat acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta
    10681 tctgcaaaaa aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa
    10741 gaaaattgcc agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct
    10801 ttattattta ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg
    10861 agatgaatct gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt
    10921 acctgaagca gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta
    10981 cgaaaacgaa gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt
    11041 caagacattc ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact
    11101 ctttccagat actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac
    11161 aagcaaccat gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc
    11221 gatgaaagac gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt
    11281 tttcgaaaaa taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt
    11341 tatgcttatt cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt
    11401 tccattcttc tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca
    11461 tagacaggta tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat
    11521 accaagatga tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc
    11581 aaatttccat ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat
    11641 ggagagataa acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac
    11701 gggataatat tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat
    11761 attctcattg ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg
    11821 atataggcag ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc
    11881 gctttcggaa agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat
    11941 agcaaaataa atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt
    12001 tttgagacac ttgcagataa actgactaat atgatattga acaaattact aagagataaa
    12061 tttgctgatg ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat
    12121 tcatttaacc atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt
    12181 gtctgttcca ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg
    12241 accaaaaacg tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca
    12301 acgattggat cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc
    12361 gaaagcattt tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg
    12421 atgatcgtga gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat
    12481 tgcatacatc tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac
    12541 gcgctgagat tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag
    12601 acgagtactt aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt
    12661 tttccaaata aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa
    12721 atataaggaa ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac
    12781 aacctcagat catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta
    12841 cggacaactc ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga
    12901 tatttttgta cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta
    12961 cgatattttt ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa
    13021 attggatttc taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg
    13081 caatagcaat cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga
    13141 tccctaatcc agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt
    13201 tcgtgacgat gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca
    13261 gccttgcttc atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta
    13321 atgcaccact agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta
    13381 aaatatgcgt acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc
    13441 cgccaaatag catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat
    13501 cattcatcta aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag
    13561 tgcaattttt acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt
    13621 atgaataaac ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg
    13681 gggagctatt tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct
    13741 ctttagccta ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca
    13801 acattgtcgt gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc
    13861 atccctttga ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt
    13921 ttcataattg aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg
    13981 aagaatcgtt tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg
    14041 tcaaatgaag caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt
    14101 tggatatcga ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga
    14161 tcttctgctt ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg
    14221 acatccatga aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct
    14281 aagtttacag aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa
    14341 gtaactggtg tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct
    14401 ttgatgattt caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc
    14461 atagtgattg ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt
    14521 acaataatta gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc
    14581 cgtgttaggt ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa
    14641 gtgattcact gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag
    14701 tatactgaca ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt
    14761 cgatctgcaa ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa
    14821 aagtacattg attaaaatca tcactggtga gatcttacca gatgacggac agatccattg
    14881 gcaaaaaaat attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac
    14941 aatcgaagaa tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc
    15001 ttttctttac caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa
    15061 actccaaacg gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat
    15121 gagcagcggg ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg
    15181 tggacaacgt tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat
    15241 cttagatgaa ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct
    15301 tcaggatttt gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat
    15361 caccacccat atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa
    15421 acaagcattg aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca
    15481 acaaaaacaa atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc
    15541 aacgatggca aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc
    15601 ttcagcaagt aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt
    15661 ggaaacaact gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac
    15721 catccgcttt ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt
    15781 attaaaaaca ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa
    15841 cacaaaaatc aattactttt cacaagatct gtactgggaa aatccattag aaacaccact
    15901 acaatattta agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc
    15961 taaagcaggg ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga
    16021 acaaactaag gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga
    16081 tgaaccgacg aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa
    16141 atttccagga acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca
    16201 acgagtcatt gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct
    16261 gtgacaaaag ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct
    16321 tcggtattag ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat
    16381 tacttgatgc agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt
    16441 ctctttcctt taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg
    16501 ctaaccgtta cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca
    16561 gccgcacctc caataaagta cataaacata aataca
//
LOCUS       DAGCWU010000052        16362 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.052, whole genome shotgun sequence.
ACCESSION   DAGCWU010000052 DAGCWU010000000
VERSION     DAGCWU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.052"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..627)
                     /locus_tag="MBZ71_001320"
     CDS             complement(<1..627)
                     /locus_tag="MBZ71_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429281.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETD"
     gene            complement(806..1525)
                     /locus_tag="MBZ71_001321"
     CDS             complement(806..1525)
                     /locus_tag="MBZ71_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4429282.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1656..2807)
                     /locus_tag="MBZ71_001322"
     CDS             complement(1656..2807)
                     /locus_tag="MBZ71_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBT4429283.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3024..3764)
                     /locus_tag="MBZ71_001323"
     CDS             complement(3024..3764)
                     /locus_tag="MBZ71_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4429284.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3768..4124)
                     /gene="rsfS"
                     /locus_tag="MBZ71_001324"
     CDS             complement(3768..4124)
                     /gene="rsfS"
                     /locus_tag="MBZ71_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBT4429285.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(4148..4741)
                     /gene="yqeK"
                     /locus_tag="MBZ71_001325"
     CDS             complement(4148..4741)
                     /gene="yqeK"
                     /locus_tag="MBZ71_001325"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBT4429286.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4725..5375)
                     /locus_tag="MBZ71_001326"
     CDS             complement(4725..5375)
                     /locus_tag="MBZ71_001326"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBT4429287.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5385..5696)
                     /gene="yhbY"
                     /locus_tag="MBZ71_001327"
     CDS             complement(5385..5696)
                     /gene="yhbY"
                     /locus_tag="MBZ71_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBT4429288.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5712..6821)
                     /gene="yqeH"
                     /locus_tag="MBZ71_001328"
     CDS             complement(5712..6821)
                     /gene="yqeH"
                     /locus_tag="MBZ71_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBT4429289.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6818..7345)
                     /locus_tag="MBZ71_001329"
     CDS             complement(6818..7345)
                     /locus_tag="MBZ71_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBT4429290.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7572..8357)
                     /locus_tag="MBZ71_001330"
     CDS             complement(7572..8357)
                     /locus_tag="MBZ71_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBT4429291.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(8350..9219)
                     /locus_tag="MBZ71_001331"
     CDS             complement(8350..9219)
                     /locus_tag="MBZ71_001331"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBT4429292.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9220..10599)
                     /locus_tag="MBZ71_001332"
     CDS             complement(9220..10599)
                     /locus_tag="MBZ71_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBT4429293.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10607..11026)
                     /gene="fabZ"
                     /locus_tag="MBZ71_001333"
     CDS             complement(10607..11026)
                     /gene="fabZ"
                     /locus_tag="MBZ71_001333"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBT4429294.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11051..11527)
                     /gene="accB"
                     /locus_tag="MBZ71_001334"
     CDS             complement(11051..11527)
                     /gene="accB"
                     /locus_tag="MBZ71_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBT4429295.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11530..12765)
                     /gene="fabF"
                     /locus_tag="MBZ71_001335"
     CDS             complement(11530..12765)
                     /gene="fabF"
                     /locus_tag="MBZ71_001335"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBT4429296.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKRDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12784..13521)
                     /gene="fabG"
                     /locus_tag="MBZ71_001336"
     CDS             complement(12784..13521)
                     /gene="fabG"
                     /locus_tag="MBZ71_001336"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBT4429297.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13533..14450)
                     /gene="fabD"
                     /locus_tag="MBZ71_001337"
     CDS             complement(13533..14450)
                     /gene="fabD"
                     /locus_tag="MBZ71_001337"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBT4429298.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14557..14781)
                     /locus_tag="MBZ71_001338"
     CDS             complement(14557..14781)
                     /locus_tag="MBZ71_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBT4429299.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14834..15796)
                     /locus_tag="MBZ71_001339"
     CDS             complement(14834..15796)
                     /locus_tag="MBZ71_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBT4429300.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15806..16246)
                     /locus_tag="MBZ71_001340"
     CDS             complement(15806..16246)
                     /locus_tag="MBZ71_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4429301.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4315 a         3729 c         2796 g         5522 t
ORIGIN      
        1 atctgtttca ggatatttta atttgacaag cgaaataatc attttcagtt cttccatgtt
       61 ttctggatat tctgggtcaa ataccttgct atgacggcca atcccgccat tgtcgatcaa
      121 tcggtaaatt cgattccatt caatgaattg gtcaattgat tggaaattgc tgattggata
      181 gccgccacaa tccaaaatct ctgatacacg aaatgctagg tatgcactat cgatcacatt
      241 attttttttc gtgtctatct tctcatttct ttcgataaga attttttcag gacgatgctt
      301 caagctccac tctaatagta gaaagaaagc ctccatccga tactcttctt caaataaccg
      361 aaaggctgaa tcagaacgac gaaaaagcag cggaatcaga taattttgtc tataaatcag
      421 cattggtgcg cggtccacca tacactctaa ataacggttc agataccctg taccgacata
      481 gtttcctaat cgatagcctt caacgccgaa tcttgcgtat ttttgcgcta attttgaaca
      541 atcagttacg ttcacagcta actcttctac tttgcggtaa ccgcttaaga tacgttcaaa
      601 aggttctaaa gtgatttttg cttccatatt cttccccgtc catcttccac atacttcata
      661 tcatttcaca tgatacttat gctctcatca taccacaggt tgaattagaa aagaaaataa
      721 atcacacaaa aaagcaagac aatctgttag tgagattgtc ttgcttttcg aaaaagactg
      781 aatacttttc gttaaaactg gttgcttagt cttctaatgc cacattatgg aacacatgct
      841 gtacatcttc tagatcttcc agtgcatcaa tcattttttc aaatgtttct tgatcttctg
      901 gagatagtgt tacctcgttt tgagggatca tttggatctc agcgattgaa aaatcagtga
      961 ttcctttttc ttttaatgct tcttgtatac tatggaaatc cgctacttca ccgtaaacga
     1021 tgatttgtcc ttcttcttcc attacatcgc gaacatcgat gtctttttcc attaggtatt
     1081 caaggatctc gtcagcgtct tctcctgcaa aagcaaagat tgctgtattg tcaaacatgt
     1141 aagaaaccgc tccggaaaca cccatgttcc cgccattttt cccaaatgct gcccgtacgt
     1201 ctgctgctgt tcgattcaca ttgttcgtca atgtatcaac aatcaccatt gagccgcttg
     1261 gtccaaatcc ttcatagcgc aattcagagt atgtttcatc tcctgcacct tttgcttttt
     1321 cgattgctcg gtcaataata tgttttggta cattataagt tttagcgcga tcaatgacga
     1381 aacgtagttt ttggtttgca tgcggatcag gatcacctga ttttgctgcc gcgtagattt
     1441 caatcccaaa ttttgcataa atccggctat tattagcatc tttagcagtt ttctttgcta
     1501 cgatatttgc ccatttacgt cccatatttt cacttcgctt tctgatttga tagtttcata
     1561 acgcaaaaac gtcctgttcc attatacttg tgaatcacga ttctgtgaag ctttttttct
     1621 ttcttccttc attcttctca tttttagtca attatctata ctcggattgg gatttgcccg
     1681 aaattctgtt ccggaatatt gcctcccaat tgatagattt gatcacttct aactaacagt
     1741 tccgcttctt gtgcctcttt ttttccgatt cgggaataaa tagaaatctc ttttttttct
     1801 tttaagaatc ttcttccaac atcggtaaat ccaagtagtc gaagataatt tttattttgt
     1861 tggacaggaa tctcttgctt tttgatattc agcaagacat aacaagataa tcgttggatc
     1921 cgtgtccatg tatatctttt tgtttttacc tttgacacaa aatcctcaaa actatcagca
     1981 gttgcagctg ctgttttcat gcgcgattct attccttcgg tcatctgata gatatctgtc
     2041 agttccagca aagtagaaga taacaaccga taacgcaaat acggccaata agattcccaa
     2101 gtctgcagcg aataagttga aagatcttct tctgtctgag gcggcacaag tccagctatt
     2161 ctttttcctt cttctaacgc atggcgaatg gctgttgcac tggctatcct ctgatctgtg
     2221 atgatttttt catgatagcc tgcactttta cgtcggatcg gatgtaattc catagggtgg
     2281 acatacttgg cattttcctt tgcataactt aatcccaaga tatgatttgg cgaagaaaaa
     2341 tcaagcgtaa tatcgggata cagactgcga aaaacctcag tcattttttg cggataacta
     2401 tacgtttgat cagacagttc ttgaaatgcc tcttctatcc tatcttgatt ctcttggaca
     2461 aagcgtccga aactgctgta atcaaacgaa gtctcttctt ctgttccaaa acacaaactg
     2521 tcacagtcca gtgtttgcaa gatttttact gcacctttag caaaatagtc agctgactga
     2581 accgcccatt cgacgggaag ctccactact aaatccacac cattttgtaa agcttcattc
     2641 gcgcgatgcc atttatcgat gatagccggt tcgcctcgtt gcaaaaaatt cccgctcatt
     2701 acagcaatga cgacctctgc tcctgttgtt tttcgtgcca tttccacatg atagcgatgt
     2761 ccattgtgaa aaggattgta ctctacgata atcccgcagc ttttcatgcg catcctcctt
     2821 catagttatc gataaaaaag aaatagagac aagtaaaaaa aggcaaaaat cggaaaaaat
     2881 acccgaacta tataaactat tctcgcaaac acaatacttc tgcgacgaaa agctgcactg
     2941 caagagcaat gcctatatcg ttcggatata taatttatgt tgaagattta ttcttttctt
     3001 gttaactcga gcaggcaaaa ccgctagtct ttgtatgcta caaagaacca gcgctttgat
     3061 ttctctgatg ctgcttcgtc tgtaaaatca gaaaatgctt cgactttcga aaagccgctg
     3121 ttttccagca ttcttaaata actttccaat ggataggtac gctcttgatg tagttcatct
     3181 tcacgaataa atcgaccaga cccttcttgt tcttcaacga aaaaggtaag aaaatgttcg
     3241 atgctgtgtt tctcgcttcc tggataacta tcccacaaaa aagcaaaatt atccccttga
     3301 taatgatagc tatattctgg aaaaatttca tctacttgat aggtggagtg tacgtcaaat
     3361 aaaaatcggc cattttcttc taacgcttga tagacttcat caaacacttg ctgtacctct
     3421 tgtctatttt tcatatagca cagcgaatca gaaaaacagg taattgcctg atattgtccg
     3481 atttctgata aatcgagcat attgccttcc acaaattgga tcgatgcgtc ttcttcatat
     3541 gctcgctggc tggcaatcat caacatctct tctgaaaggt ctaaccctgt cactttatat
     3601 ccatcttttg caaaggcaac tgctaatgct ccagtaccgc atgccagttc caaaatattt
     3661 tttgtgtctt cttgtaaatg acgtttcgaa aaagctagcc attgctgata aagtgtttcg
     3721 tccatcactt catcgtaaat gaaagcaaat gtttcataag ccatatgtta atccaaccat
     3781 tcactaagat cgactagcgg agcatctgac catagttttt ccagattata gaaacttcgt
     3841 tctggtgcat ggaaaacatg tacgatcaca tctccaagat cgatcaatac ccattttccg
     3901 ccttcttttc cttcaatacg ttttacttca tatttgtttt cttcttcttt atcaatgatt
     3961 tcttctgtaa ttgcgttgat ttgacgttcg ctatttgctg aacagatcat gaaataatct
     4021 gccaacaagg aaacatttcg aatgtctaaa gcgataatgt cttctgcccg ttttgaatct
     4081 gctgcttcaa ccgcaatttt taattgttct ttactatcta tgatgatttc ctcctaatat
     4141 gagcagctta tttttttgct acccagtaat tatatgtttc gattgttttt ggataaatct
     4201 gttgttcttg cgcaagcaaa tgggccaatg tatgttttgt ttcaaatgct acagcttcgt
     4261 ctagatcagc ccatgcaatg gctcgtgcat cttgtacccc aggaaaatct cgacctggtt
     4321 caatatagtc tgctacataa atgattttgt caaggagact catcttagca gccccagtcg
     4381 tatgcacccg gattgcatga agaatttcta catcggtgat tcctaattcc cgctctacaa
     4441 acgaagcgcc aacaagacca tgccagattg cattattata attcaataac tctggatcaa
     4501 aaccgtctct tacgattacc attttgaact cctcatctgg tcgttctttt gcataatcat
     4561 gtgtcaatgc agcaatacta gctttttcag gagaagcacc gtattttttg gcaagagcca
     4621 cggctgtttc ttctactcca agcacatgct tgaagcgttg ttcgctcatt ctcatctgta
     4681 ttttttgcat caggttttct ctagaataag cagtatattc attactatag tccatattca
     4741 tacaaccctt cattttgaat ataatcaatt actttatctg gaacaaggta gcggatagaa
     4801 cagccttctt tgatcttttg acgtattttc gtggaactga tgtcgatctc aggtacatct
     4861 acccaaatta caggatatgg agtatccgtt gtgtatcctg gtcgacgtat acccacaaag
     4921 ttgaccatag aagtcagctc atcgatttta taccattttg gcaaatattc taccatatcc
     4981 ccaccgataa taaagtaata atctgtatcc ggattgtttt gcgtcaattc cttcatagta
     5041 tcataggtgt aacttttccc accacgagct aattcaatcg tttcaatctg caaaaaagga
     5101 ttgtcttcca cagctaattc caacatgttg agtcgatgtt ttgcatcaat cgtctgtttc
     5161 tcatcaacat gaggtggctg ataagaaggc atcaaaaata cgcggtcaag acctaaattc
     5221 cggccagctt gttcagccat cactaaatgt gcaaggtgaa cgggattgaa tgttccgccc
     5281 aagatcccta cttgcttttt cttctctaag aattgaggca tttcttcagg aaataattgt
     5341 gaacgaacaa atgtcttagc ttgcgtattc atcatggtca ctccttatat tgcttttact
     5401 tcttttgata gcctttgata cttttctttt gtggaaggtt tgtataatac aataacacgt
     5461 ccaatgattt ggacgatttc gcaatctatt tctttttcta atacttgagc tgcttcttct
     5521 gcaatctcat ctgtattttg aagtaacgat actttgatca gttctctttt ttctaaagct
     5581 tcttcaattt gaacgatcat tgctgaattg atcccacctt ttccgatttg aaaaatcggc
     5641 tgaagatgat gcgctttgct acgtaaaaaa cgcttttgtt ttcctcttaa actcataatt
     5701 ttccttcttc cttaaatcaa tgctttgcgt ttcaatacat ctacaccctt tggcgcccag
     5761 cccgcaatta ctactggcgc tgttacagtg atccagccta gaccggcaaa aacgatgtct
     5821 gttttttctt tcacagaaaa ctcaaaacga acaagctccg gaaatgcctc gacttcatca
     5881 ggacgaggag gctgtaataa tccacctaca tgtttttgat aaaattcgtc tgctttttcc
     5941 agtttcgttc gatgaatatt tacatcattt gacacataag caataaatga agcacgattg
     6001 ccgctgataa aatcaaatct tgcaagtccg cccaagaaaa gtgtttgccc ttcattcagc
     6061 tgataaactt taggtttgat ttcttttaac ggtgcaatga tccgcaaatc ttttttacct
     6121 aagtagtgcg ccatttgatg gcgatggata attcccggtg tatcaatcaa aaaatgcccg
     6181 tcatctaaag gaatctcgat tttatccaag gtcgttcctg gaaactgcga agttgtgatt
     6241 acatcttgta ctcccgctgt atttttgatg atttgattga taagcgtcga ttttccgaca
     6301 tttgttactc cgacgatata gacatcccgt ccatcgcggt attcttcgat tttttctaat
     6361 agttccgtca tctcatgaga tttcttagca ctagttaata atacatctac tggacgaatt
     6421 cctgcttcgt aagcacgttc cttcatccat tgtgttaatt tagttttttt caatgattta
     6481 ggtaatatat ccactttgtt cccaactaaa agtactggat tgtcaccgac aaaacggtgt
     6541 agtcctggga ttagtgaacc attgaagtca aaaatatcca ccacattgac gatcaatgca
     6601 tctttctgcc ctaattcatt cagtaagcgc aaaaagtcgt catccgtcaa tgagacatct
     6661 tggatctcat tataatggcg taatctgaaa caacgctgac aatagacttc cccggaagct
     6721 aaaccttttt ccaaagccgt ttgcggcgtg tatcctaatt caccgggttt gtttgtttgt
     6781 ataacggcac cacagccgat acattgtaat tcttctgtca ttcgattcca cctcgccagc
     6841 ccatatcagg gtgttttgct aatagatagg ccataattct tttttcccaa aaacgattaa
     6901 attgtgtctt ccaactatct gttgcgataa tgggttttac aaggatgcta cgtatccctg
     6961 cacgattggc tccacgtata tcagtcataa tctgatcgcc taccattact atctcatcat
     7021 ctgctaaatc caattgtttc tttgctatgt tgatccctct tgctaaaggt ttcattgctc
     7081 gtgcaacata caaaagatca aatttttcaa tcgctcgcgc gacacgtttt gaattattgt
     7141 ttgaaacaac aacaacaggg attcctgcat ttttcatttc taaaatccaa gtaagcaact
     7201 cttctgttcc atctggatta ttccatgcaa tcaacgtatt gtctaaatcg gtcaatactg
     7261 ctttgatgcc tagattcttt aattgggcag gtgtaatctg atagatggct tctaccatcc
     7321 atgttggttt gtaatttgaa aacatgctgt ctcctcatct gcaaaaaatg aaggagcaca
     7381 taaagtactc ctcaaccccg tattatactg catatcagca aaaaaaacta cctctatact
     7441 cgttcgtctt tcagaaaaag catttcttta caaaatgacc aaaaataaag attgatcaca
     7501 gaaaaattta gaaacaaaaa acaactaagc gagcaagacc attctttact ctttgcttag
     7561 ttgttaaaca cctaatattg gcggaacctc ttgtatcttt tttccagtaa ttcatgctgg
     7621 tcgaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact
     7681 ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct
     7741 aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta
     7801 ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct
     7861 tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca
     7921 ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca
     7981 atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg
     8041 atcaatgtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt
     8101 cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt
     8161 cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca
     8221 atcgcttcgt catctgcaaa caaacgatca ccatggcatt cctcgaaaga atcgaagatc
     8281 tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca
     8341 tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg
     8401 gattttctgc ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa
     8461 gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg
     8521 tcttccagca aaaccgatca agctttgggg ttctgctaga atgatatcgc cttccattgc
     8581 aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc
     8641 ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat
     8701 tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt
     8761 tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat
     8821 gataaaatta ggatccatca ctccaataat gaagggaatc ccttgaatgg ttgctttacc
     8881 agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata
     8941 ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt
     9001 ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg
     9061 agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca
     9121 ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg
     9181 attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt
     9241 ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc
     9301 tatgactgca gggtgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc
     9361 tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc
     9421 atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta
     9481 aactgcactg tctacccgca gtcccatccc acctgctgga agcagtaagt tttggatctt
     9541 ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc
     9601 gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc
     9661 cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg
     9721 gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat
     9781 cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc
     9841 ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt tttgattatt
     9901 acgttgcaaa gaacaatctc gttctcctaa atgaatgaca ttgccaaaat gatcacctaa
     9961 aatctgaact tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt
    10021 tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt
    10081 tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg
    10141 ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact
    10201 tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc
    10261 cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat
    10321 ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt
    10381 tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc
    10441 cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc
    10501 tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc
    10561 gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa
    10621 aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc
    10681 cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa
    10741 ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt
    10801 gttcaagaga aagtaatgca actgctcctg cttgagccat cgcttcaaca attaataccc
    10861 ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat
    10921 ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca
    10981 ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgat ttccctcttt
    11041 cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact
    11101 cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca
    11161 gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt
    11221 tgaaagctgg tttgtcgggc gccggttgca gataaacgat tcccacgatt ggcgaaacaa
    11281 cctcgattcc atcaaatact gccgattctt tttctttcat tgtttctgga gcagttgttg
    11341 cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca
    11401 tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca
    11461 aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg atttcattga
    11521 tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc
    11581 taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt
    11641 caagtcaatc tcaggatctc gtttttctac attgatcgta ggtgggataa attgatgttc
    11701 taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt
    11761 catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat
    11821 tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc
    11881 gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagacgcacc
    11941 agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaat
    12001 gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag
    12061 aacacctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc
    12121 acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat
    12181 ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat
    12241 gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt
    12301 acaaatacct tttgcaccta cgcgcaaagc aatatttcca gctgccatgt tagcaatcgc
    12361 cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg
    12421 gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg
    12481 attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc
    12541 atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg
    12601 gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat
    12661 cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc
    12721 aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt
    12781 ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg
    12841 attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat
    12901 cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt
    12961 tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc
    13021 cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg cctgaccaat
    13081 atttccaatc aacccagata cactagagag attaatgatg gccccttctc gctgcttcat
    13141 catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatcaagaca
    13201 ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt
    13261 gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc
    13321 tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat
    13381 ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc
    13441 ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac
    13501 attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat
    13561 gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg
    13621 cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta
    13681 tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt
    13741 gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag
    13801 aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta
    13861 tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt
    13921 aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta
    13981 taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt
    14041 tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc
    14101 atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta
    14161 gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca
    14221 tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat
    14281 tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga
    14341 atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc
    14401 ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc
    14461 aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt
    14521 caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta
    14581 agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc
    14641 atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg
    14701 gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc
    14761 ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt
    14821 ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc
    14881 ctgtaaatat tactttctgc tgttttccca aggtgatctt tccactagaa actgcttcat
    14941 ccaataagat agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga
    15001 acttttcttg cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct
    15061 gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa
    15121 cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt
    15181 ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac
    15241 ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta
    15301 agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga
    15361 ttagcttaga tagtgtttca ccaccaatca ctagtccctt agttgagccg cagcggatca
    15421 ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag
    15481 cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat ggcgtcgtgt
    15541 aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct
    15601 gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac
    15661 atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct
    15721 tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca
    15781 ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt
    15841 tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga
    15901 ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc
    15961 ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta
    16021 ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt
    16081 ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg
    16141 acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga
    16201 agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac
    16261 taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat
    16321 gcaaccttaa aagatcattt ttttactata tgagcttttt tt
//
LOCUS       DAGCWU010000053        16301 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.053, whole genome shotgun sequence.
ACCESSION   DAGCWU010000053 DAGCWU010000000
VERSION     DAGCWU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.053"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(31..894)
                     /locus_tag="MBZ71_001341"
     CDS             complement(31..894)
                     /locus_tag="MBZ71_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4429302.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1090..3189)
                     /locus_tag="MBZ71_001342"
     CDS             complement(1090..3189)
                     /locus_tag="MBZ71_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBT4429303.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3409..4518)
                     /gene="rpoD"
                     /locus_tag="MBZ71_001343"
     CDS             complement(3409..4518)
                     /gene="rpoD"
                     /locus_tag="MBZ71_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBT4429304.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4542..6383)
                     /locus_tag="MBZ71_001344"
     CDS             complement(4542..6383)
                     /locus_tag="MBZ71_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBT4429305.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6565..8880)
                     /locus_tag="MBZ71_001345"
     CDS             complement(6565..8880)
                     /locus_tag="MBZ71_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4429306.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9065..9925)
                     /locus_tag="MBZ71_001346"
     CDS             complement(9065..9925)
                     /locus_tag="MBZ71_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBT4429307.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(9984..10616)
                     /locus_tag="MBZ71_001347"
     CDS             complement(9984..10616)
                     /locus_tag="MBZ71_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBT4429308.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            10783..11544
                     /locus_tag="MBZ71_001348"
     CDS             10783..11544
                     /locus_tag="MBZ71_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4429309.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11570..14161
                     /gene="mprF"
                     /locus_tag="MBZ71_001349"
     CDS             11570..14161
                     /gene="mprF"
                     /locus_tag="MBZ71_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBT4429310.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14349..15536)
                     /gene="tuf"
                     /locus_tag="MBZ71_001350"
     CDS             complement(14349..15536)
                     /gene="tuf"
                     /locus_tag="MBZ71_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBT4429311.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLTE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15904..16260
                     /locus_tag="MBZ71_001351"
     CDS             15904..16260
                     /locus_tag="MBZ71_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4429312.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4457 a         3381 c         2881 g         5582 t
ORIGIN      
        1 agctagttaa gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac
       61 accgttttct tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc
      121 tcctttgctg ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt
      181 gatttgctga tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat
      241 agcttcatat gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat
      301 cactcgacct tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat
      361 aaacccaaga tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc
      421 tgttcctatc acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa
      481 tattttttca tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat
      541 catcaagcga tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga
      601 tacgacaaag tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt
      661 agcagtaacg attccaaaac cgtaatgacc gatacggctt ttaggaattt gtgtagtgat
      721 ccgattttca tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg
      781 ttcaccttct gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt
      841 ttcattttca tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt
      901 ctccctctat agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat
      961 ctacttgaat agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt
     1021 gtcatactcc cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt
     1081 tgcctaaaat taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac
     1141 gatgtaaaat ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt
     1201 ggcatataga ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc
     1261 tccttcaacg atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg
     1321 tgcccacgtc catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat
     1381 tggctgattc gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc
     1441 ttgtgcgacc gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt
     1501 tgcttgttct tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt
     1561 tttctcttta tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga
     1621 tagatctgta tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt
     1681 tgctgaatcc actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg
     1741 caattcagca tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc
     1801 tgtgttatcc gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc
     1861 tagatgatca tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg
     1921 gatcttagat tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt
     1981 aagactggat aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat
     2041 aaattgatgg tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg
     2101 ttgctgtctt tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata
     2161 agcccagctt cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt
     2221 tgattttttc ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt
     2281 tttttcagta tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg
     2341 gttctcttct ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt
     2401 ttcagcatcg gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc
     2461 gagtatttct tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt
     2521 ttcagtaaat acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt
     2581 ttctttacct gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg
     2641 ctctactttg ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg
     2701 ctcttctttt gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc
     2761 ctcattatcg acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt
     2821 ttcttcttga ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc
     2881 atttttctct atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc
     2941 tttttcgaat aattccatta cagattctag aggtaaatct ttcaattccg accattcgat
     3001 tggatcgttt aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc
     3061 tgcatctgta tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg
     3121 atgaccgcag tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca
     3181 ttttttcatc acttagcgat cggctccttt actaggttta cgctaccatt taatctatca
     3241 tattctatac gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat
     3301 ttccttccta tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa
     3361 ataaaccaaa cagttttact gatttctctt ctggtcaatg gtccacgctt attctaagaa
     3421 atcttttaat tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat
     3481 ctgacgaata cgttcccgag taacaccaaa cactttccct acttcttcca atgttctcgt
     3541 ccggccatca tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt
     3601 atctagtaca tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact
     3661 tgttgcttcc tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg
     3721 cgtttcaagg gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg
     3781 aagatccatt tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa
     3841 ttgacgttgg atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat
     3901 cgtacgtgct tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata
     3961 ggttgaaaac ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat
     4021 gtttccttct tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat
     4081 acttactact aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc
     4141 ttcgattttc aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat
     4201 ttctttcaga tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc
     4261 ttctgttttt gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc
     4321 accattttca tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat
     4381 ttcatctgca ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac
     4441 ttgtcgtttg ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt
     4501 atctgttcca tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac
     4561 tgtttcgtta aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt
     4621 cgtctggctt cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg
     4681 ctgatcacat ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa
     4741 tattcgattt ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct
     4801 gcaaggttga attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca
     4861 tatgcatcat gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct
     4921 ttaaatgcac gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt
     4981 ttccgtgcag tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga
     5041 ttttgctgat ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc
     5101 tgaaactctg ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg
     5161 ctaagttcaa ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt
     5221 ttcccttgtt ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact
     5281 aattcttgga aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct
     5341 ggaacgacta cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga
     5401 ttggttgctt caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc
     5461 ttttccattt gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt
     5521 ttgataccag cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct
     5581 ccgtttttcc ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg
     5641 aacagttctg tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca
     5701 ggtgcaagcc agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac
     5761 atcactcttt gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca
     5821 gagcgttcta acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa
     5881 cgctcattag gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga
     5941 ctttgcaaat attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag
     6001 atttccgctg ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg
     6061 acagctatag tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact
     6121 tttaccactg cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt
     6181 ccgcctttac cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac
     6241 ggtgtacgtt cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac
     6301 tggacatatt gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc
     6361 tcttgaggaa tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt
     6421 aacagaagat atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga
     6481 ttttaaagga cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat
     6541 aacacatttc tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat
     6601 atcagccttc cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta
     6661 tcctacgctc tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc
     6721 tacatatatc cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata
     6781 acaagctttt acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt
     6841 agtttcagca tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac
     6901 tactacaagc aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt
     6961 gatttttctt ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa
     7021 atcaatcagc gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat
     7081 aatcgataaa ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac
     7141 attgttgacc actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc
     7201 caacaatacc agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct
     7261 gcaatctgct tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt
     7321 tctgccgctt tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc
     7381 aaaaaccgta tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag
     7441 atcgacatac gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa
     7501 aggattgtct ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt
     7561 ttcttgagcg ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa
     7621 tgctggtttt tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata
     7681 gccattcgct tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc
     7741 aggcgctcct acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc
     7801 agaagaaaat gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct
     7861 catagcccgc atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc
     7921 tgggatggct ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc
     7981 ttcagtaatg gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat
     8041 tgaatacatg tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa
     8101 tgcaatactt attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga
     8161 tgcaataaca gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat
     8221 gatccccaaa ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac
     8281 aagttcagaa cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt
     8341 atctgcacct actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga
     8401 aagcaattca gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc
     8461 atttgcttct gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc
     8521 gatttttacc aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc
     8581 ccggatcact agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc
     8641 atgtatttct tgataaattc caataacgat attgacgaca ataattgcta aatacgccat
     8701 gttggaatac gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa
     8761 taacgtcaat atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta
     8821 gtcattttgc tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat
     8881 tttctttttc tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg
     8941 ttccaagatt aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt
     9001 acaaaaaaga tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct
     9061 atctttactt atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta
     9121 taaaaagaac aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa
     9181 tgatcataat cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg
     9241 cctttgagaa aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa
     9301 ttccgatcgg cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat
     9361 acaatcccat atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac
     9421 caatcaatcc gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata
     9481 attgagcgcc gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca
     9541 catcagaagt atctcttcct tttttccctg cttctttcca taacgcagga acagttccag
     9601 cgatacatcc gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga
     9661 ctaaaaaaga aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca
     9721 aaacattttc tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca
     9781 tgccaaagac agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga
     9841 taaacattcc cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat
     9901 tttccatttt ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc
     9961 atgattgaac atttcctgct attttactta aagacttttt cataggtctc tattattttt
    10021 tcattcattt tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg
    10081 ccattgtaaa gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct
    10141 aaaagtgggc tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg
    10201 ccatgatttt ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc
    10261 cataaatcgc atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct
    10321 tgatcaatac tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag
    10381 ctctgcatga gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa
    10441 ttctcgtagt tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt
    10501 tccactcgtt ttaccgaacg ataagttctt cctcctaaaa acaacaccgc accaataacc
    10561 gtcagaatga tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca
    10621 tcacctagtt attttgacat ctttttttat cttactattt ctttatcttc ttggctattt
    10681 cttccttgat ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc
    10741 ttacgaaaat gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt
    10801 atactttaca cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat
    10861 gggagcgaaa tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc
    10921 aatgacggca gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc
    10981 gtgtatctag tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat
    11041 tttcagttaa tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca
    11101 gattttcttg gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct
    11161 aaaaaagttc atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt
    11221 tctgccagag tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc
    11281 ccaagtcttc gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca
    11341 gaaaatgatt taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta
    11401 atcaagctaa aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt
    11461 gtgaaagaac aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt
    11521 cttcaatttc tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa
    11581 taccatacaa tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt
    11641 cattgtcatt attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt
    11701 aggagaaacg tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct
    11761 tatctctgtg ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa
    11821 gcagaatcct cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc
    11881 cggtttcggc gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga
    11941 gagcagaaac gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc
    12001 tatttacagt ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa
    12061 gcaatattgg atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac
    12121 tactttcaaa aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt
    12181 gctagtttca ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt
    12241 aatggaaatc cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat
    12301 aggaatcgtg tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg
    12361 gttgtccgct attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct
    12421 gttctactac atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc
    12481 cacttttgat cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa
    12541 aatcgtggtt gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc
    12601 gcaagctttt acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat
    12661 tatccaattt cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc
    12721 agcacgtgtg aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta
    12781 tgttttatta agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat
    12841 cattcttgct tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg
    12901 tacgatcgat ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata
    12961 caatctgcca gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc
    13021 tgaaaagatt tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct
    13081 atttgttcat ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa
    13141 ggccctgaag atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa
    13201 agataagcgg atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc
    13261 ttgcttcaat aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc
    13321 agaagcaatc gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta
    13381 cgaaacaagc gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat
    13441 gggggaagaa gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg
    13501 cactcgtgct gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc
    13561 accattttct actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc
    13621 acgaaaggaa aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc
    13681 tattgcagtt gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac
    13741 ttacacgaac aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc
    13801 ttctggcagt atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat
    13861 ccaatggttc aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt
    13921 tttacaggaa aggattgcat acttggttta cgagtttggt tctcattttt attctttcca
    13981 tggattaaga gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata
    14041 ttctagagat agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc
    14101 agttgagcag acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta
    14161 aaaactgcat accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg
    14221 aaagttcatg cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca
    14281 gaaatcctgt ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt
    14341 cgcgtttctt atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca
    14401 cgaattgaga atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc
    14461 gtcacgttgt caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta
    14521 acatcagttg tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt
    14581 ccaccttctt cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg
    14641 gaacctggtt tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac
    14701 aacacgccta cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca
    14761 cctgttacaa ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg
    14821 acacgaacag caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag
    14881 aagacgtctt ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata
    14941 tattcatcta ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc
    15001 aatgctttta atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg
    15061 gtcaataact cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa
    15121 tcaactttgt tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg
    15181 tgttcacgtg tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc
    15241 atttgtgctg cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata
    15301 tgcgcgtagt ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca
    15361 cgttcacgtt cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct
    15421 tttttgccta atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt
    15481 ccaatcgtac caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt
    15541 tacaaacctc ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata
    15601 gtcttttttt gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact
    15661 aagcttatta tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg
    15721 cgaaaaaagt accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt
    15781 ccttaacttt cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt
    15841 gccaacaact ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag
    15901 tacatgaaaa aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt
    15961 gcagcttatc attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc
    16021 gaaatgtcag ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa
    16081 gcggcttata ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat
    16141 ccattaaaga tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc
    16201 tgggaagaaa taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag
    16261 taaatggtct taaaatttta ttagataaaa agaggctggg a
//
LOCUS       DAGCWU010000054        15798 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.054, whole genome shotgun sequence.
ACCESSION   DAGCWU010000054 DAGCWU010000000
VERSION     DAGCWU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.054"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(135..512)
                     /locus_tag="MBZ71_001352"
     CDS             complement(135..512)
                     /locus_tag="MBZ71_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429313.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            768..1496
                     /locus_tag="MBZ71_001353"
     CDS             768..1496
                     /locus_tag="MBZ71_001353"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBT4429314.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1496..1750
                     /gene="purS"
                     /locus_tag="MBZ71_001354"
     CDS             1496..1750
                     /gene="purS"
                     /locus_tag="MBZ71_001354"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBT4429315.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1752..2423
                     /gene="purQ"
                     /locus_tag="MBZ71_001355"
     CDS             1752..2423
                     /gene="purQ"
                     /locus_tag="MBZ71_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBT4429316.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2424..4646
                     /gene="purL"
                     /locus_tag="MBZ71_001356"
     CDS             2424..4646
                     /gene="purL"
                     /locus_tag="MBZ71_001356"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBT4429317.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4631..6070
                     /locus_tag="MBZ71_001357"
     CDS             4631..6070
                     /locus_tag="MBZ71_001357"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBT4429318.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6093..7145
                     /locus_tag="MBZ71_001358"
     CDS             6093..7145
                     /locus_tag="MBZ71_001358"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBT4429319.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7142..7720
                     /gene="purN"
                     /locus_tag="MBZ71_001359"
     CDS             7142..7720
                     /gene="purN"
                     /locus_tag="MBZ71_001359"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBT4429320.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7717..9252
                     /gene="purH"
                     /locus_tag="MBZ71_001360"
     CDS             7717..9252
                     /gene="purH"
                     /locus_tag="MBZ71_001360"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBT4429321.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9268..10542
                     /gene="purD"
                     /locus_tag="MBZ71_001361"
     CDS             9268..10542
                     /gene="purD"
                     /locus_tag="MBZ71_001361"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBT4429322.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10664..11578
                     /locus_tag="MBZ71_001362"
     CDS             10664..11578
                     /locus_tag="MBZ71_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBT4429323.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11711..13276)
                     /locus_tag="MBZ71_001363"
     CDS             complement(11711..13276)
                     /locus_tag="MBZ71_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4429324.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13269..14720)
                     /locus_tag="MBZ71_001364"
     CDS             complement(13269..14720)
                     /locus_tag="MBZ71_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4429325.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14734..15750)
                     /locus_tag="MBZ71_001365"
     CDS             complement(14734..15750)
                     /locus_tag="MBZ71_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4429326.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
BASE COUNT         5016 a         2720 c         3546 g         4516 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
      661 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca
      721 aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag
      781 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
      841 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
      901 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
      961 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
     1021 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
     1081 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
     1141 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc
     1201 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
     1261 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
     1321 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
     1381 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
     1441 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
     1501 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
     1561 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
     1621 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
     1681 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
     1741 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
     1801 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
     1861 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
     1921 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
     1981 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
     2041 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
     2101 tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
     2161 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
     2221 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
     2281 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
     2341 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
     2401 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
     2461 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
     2521 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
     2581 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
     2641 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
     2701 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
     2761 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
     2821 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
     2881 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
     2941 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
     3001 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
     3061 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
     3121 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
     3181 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
     3241 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
     3301 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
     3361 cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
     3421 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
     3481 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
     3541 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
     3601 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
     3661 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
     3721 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
     3781 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
     3841 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
     3901 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
     3961 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
     4021 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
     4081 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
     4141 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
     4201 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
     4261 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
     4321 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
     4381 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
     4441 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
     4501 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
     4561 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
     4621 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
     4681 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
     4741 aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
     4801 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
     4861 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
     4921 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
     4981 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
     5041 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
     5101 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
     5161 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
     5221 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
     5281 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
     5341 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
     5401 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
     5461 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
     5521 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
     5581 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
     5641 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
     5701 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
     5761 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
     5821 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
     5881 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
     5941 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
     6001 tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
     6061 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
     6121 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
     6181 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
     6241 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
     6301 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
     6361 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
     6421 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
     6481 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
     6541 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
     6601 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
     6661 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
     6721 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
     6781 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
     6841 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
     6901 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
     6961 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
     7021 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
     7081 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
     7141 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
     7201 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
     7261 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
     7321 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
     7381 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
     7441 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
     7501 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
     7561 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
     7621 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
     7681 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
     7741 tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
     7801 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
     7861 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
     7921 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
     7981 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
     8041 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
     8101 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
     8161 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
     8221 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
     8281 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
     8341 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
     8401 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
     8461 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
     8521 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
     8581 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
     8641 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
     8701 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
     8761 ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
     8821 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
     8881 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct
     8941 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
     9001 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
     9061 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
     9121 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
     9181 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
     9241 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
     9301 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
     9361 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
     9421 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
     9481 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
     9541 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
     9601 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
     9661 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
     9721 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
     9781 aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga
     9841 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
     9901 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
     9961 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
    10021 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
    10081 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
    10141 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
    10201 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
    10261 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
    10321 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
    10381 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
    10441 aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
    10501 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
    10561 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
    10621 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
    10681 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
    10741 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    10801 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    10861 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    10921 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    10981 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    11041 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    11101 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    11161 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    11221 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    11281 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    11341 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    11401 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    11461 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    11521 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    11581 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    11641 tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg
    11701 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    11761 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    11821 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    11881 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    11941 tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat
    12001 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    12061 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    12121 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    12181 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    12241 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    12301 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    12361 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    12421 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    12481 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    12541 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    12601 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    12661 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    12721 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    12781 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    12841 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    12901 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    12961 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    13021 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    13081 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    13141 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    13201 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    13261 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    13321 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    13381 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    13441 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    13501 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata
    13561 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    13621 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    13681 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    13741 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    13801 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    13861 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    13921 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    13981 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    14041 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    14101 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    14161 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    14221 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    14281 acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata
    14341 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    14401 aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta
    14461 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    14521 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    14581 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    14641 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    14701 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    14761 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    14821 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    14881 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    14941 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    15001 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    15061 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    15121 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    15181 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    15241 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    15301 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    15361 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    15421 tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    15481 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    15541 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    15601 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    15661 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    15721 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    15781 ctatcttatc agataaaa
//
LOCUS       DAGCWU010000055        15353 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.055, whole genome shotgun sequence.
ACCESSION   DAGCWU010000055 DAGCWU010000000
VERSION     DAGCWU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.055"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..248)
                     /locus_tag="MBZ71_001366"
     CDS             complement(<1..248)
                     /locus_tag="MBZ71_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4429327.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNG"
     gene            278..688
                     /locus_tag="MBZ71_001367"
     CDS             278..688
                     /locus_tag="MBZ71_001367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429328.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            769..1389
                     /locus_tag="MBZ71_001368"
     CDS             769..1389
                     /locus_tag="MBZ71_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429329.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            2090..2374
                     /locus_tag="MBZ71_001369"
     CDS             2090..2374
                     /locus_tag="MBZ71_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429330.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2377..3294
                     /locus_tag="MBZ71_001370"
     CDS             2377..3294
                     /locus_tag="MBZ71_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4429331.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            3287..4069
                     /locus_tag="MBZ71_001371"
     CDS             3287..4069
                     /locus_tag="MBZ71_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4429332.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            4070..4750
                     /locus_tag="MBZ71_001372"
     CDS             4070..4750
                     /locus_tag="MBZ71_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4429333.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4743..5639
                     /locus_tag="MBZ71_001373"
     CDS             4743..5639
                     /locus_tag="MBZ71_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4429334.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5834..7483
                     /locus_tag="MBZ71_001374"
     CDS             5834..7483
                     /locus_tag="MBZ71_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBT4429335.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            8077..9012
                     /locus_tag="MBZ71_001375"
     CDS             8077..9012
                     /locus_tag="MBZ71_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429336.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            9005..9775
                     /locus_tag="MBZ71_001376"
     CDS             9005..9775
                     /locus_tag="MBZ71_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4429337.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            10191..11174
                     /locus_tag="MBZ71_001377"
     CDS             10191..11174
                     /locus_tag="MBZ71_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBT4429338.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            11621..12658
                     /locus_tag="MBZ71_001378"
     CDS             11621..12658
                     /locus_tag="MBZ71_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4429339.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(12747..13070)
                     /locus_tag="MBZ71_001379"
     CDS             complement(12747..13070)
                     /locus_tag="MBZ71_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBT4429340.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(13057..13764)
                     /locus_tag="MBZ71_001380"
     CDS             complement(13057..13764)
                     /locus_tag="MBZ71_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBT4429341.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            13850..14191
                     /locus_tag="MBZ71_001381"
     CDS             13850..14191
                     /locus_tag="MBZ71_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBT4429342.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(14362..15210)
                     /locus_tag="MBZ71_001382"
     CDS             complement(14362..15210)
                     /locus_tag="MBZ71_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429343.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         4889 a         2646 c         3245 g         4573 t
ORIGIN      
        1 ccgttgcggt cgcgcggaat ctggagatga agttccccgt actcggtctt gaccgtacgg
       61 tcataggagc cgttacgaga attccccgtg ttaaaaccaa tgcgatcgta cttttcgtaa
      121 tcgaggaaag ccgttaactc cgttttgaga agcgtattga cagctttttc caaatggaca
      181 cggaataatt cattcaaatc gcctttgttc gctagagtct ttagaatttc tgtagtaaaa
      241 tcgttcatat ggaagtcctc ttttctgtga atgtgttgtg gtaactctat tctacagaag
      301 ggacttcctt ttttctattt acacaaaata ttttacactc tcgtcaaata taaaaactag
      361 tacgaaatta atttgtaaat tatttaagct tgataaaata agcaatatcg atgatgtaaa
      421 gtccaagata ttaacaagaa aagtaagcga accagtaaag ctaacggcac aagataaaaa
      481 aaatggaatt gttctttatg gtgatgatgc aaagtataac tataattggg atacctcctc
      541 agaagctgag gtattaagta ttcctactac cgatacttca ggggaaaaag tcacctttaa
      601 aataaatttt ataacggcaa tttctaagtt ggagtatggg aagcaaaaaa agagagataa
      661 caatgatcaa tatctaccta aaaaataacg aattaatgat gcatatgtta aagttgtttt
      721 tttgaattaa acaactcaat ttattcagtt atatgttcat cgcagcaagt gaacattaat
      781 cgagttataa aattaatagg agctaaaaat ataaataaag ttcgatcaga atttattttt
      841 ccagaagatt tttttaactg gtttttttat caattcagtg aatgcaagcg actcttaggt
      901 gagaattatt acttaaaagg aatcggcgga tttatgggga atgtggctga tgaacaaaat
      961 attataaaag gaatatctga acaaacatca gagcttacag taactaaagc atttataagt
     1021 aatggtgaaa gtttgaaaaa tgtaactgcg cgaattagaa aagaaaatga agtagacatt
     1081 gtttttggaa ttgaccatga atgtaattca gtgatatatg taacacaatc agcaaattta
     1141 attctttttg agaatggttg caaggacgat tttatggtgg tctatttgta cgatatacta
     1201 attccaaaac ttcgttcctt atatatgtct tctgaaagta tttttgtgag tgaacaaaag
     1261 ggatctttct cgaaaaaaat aggtattgaa gtaattgaat cgattatgtg tcataatcat
     1321 attacgctag aagaattaga aattttgata gggcaaaaaa ataataacac gaaaatacaa
     1381 gtttcttaga aatcattttt tattgagata tctagataaa tgatttattc ttcaatcaac
     1441 caactcgcca agctgaggta atgttttgag gtaatgataa catacaactt tttataccct
     1501 agtaaaactc tataaacaaa gacttattaa tttcgcatga aatacattcc tgtaaaatgc
     1561 aataaaatag agttttgttt aaccaggtct taagaaagcc cgtaaagctt cacagttctc
     1621 aaaacgttaa ttgcgattat cgttcgagtt cacgagttac gagaatcgta ctgggcgaag
     1681 caatgctgag cgcagttacc ttaatcctaa cttttctggc actttccaca atttggaaag
     1741 tgcctttttt gtgtttttag ctatgttaat aggtaggatt cttggtctat aatatcattc
     1801 gatctataaa caaacgggaa aaagataaag tatcatcaaa caatattatt tatccgcttc
     1861 tgaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaatttaca aaaattgctt
     1921 ctcatgtttg ataaatattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttat
     1981 tcggaagaag ggattgtgaa aagtcttttg tcataacctc ttatttggta gctaattgta
     2041 aatcccaaac ggcatgtatt atttttcata agtccgtttt taaggattaa tgttttcttt
     2101 tgctttttat atggtctggc ttcagcgaga agttccaacg catatacaag gacgtctgat
     2161 gtcaacgata acaacgatta ccatggtggc cagtccactt ggattagcac tgtttggtgc
     2221 gctctttgat tggcagacaa ctcaaccacg tctgcgagac agtctgattt ttcttgaaat
     2281 tcaattggaa aatttacact ttctttatac atttctggct gctttcttta tacccgtcaa
     2341 ctatacttca cttatcaata agaaagaagg ataagtatgg attttggaaa tgaaacggtc
     2401 ttgaaagcaa ctggtataac caaaaaatac ggtgctgcca aagcattaga taaagtatcg
     2461 atcgaaatca aacgtggaat gatctatggt ttgattgggg agaatggagc gggtaagtct
     2521 acctttatga gaactattat gggtttgatc tcgattgatg aaggttcgat cgaattattt
     2581 ggaacgacgg atttgcaggc agcaagacga cgtatgggtc aatcgattga aacccctgct
     2641 ctttatccag aattaactgc aagagataac ctacgtatcc aagcagcgaa cggcggtgtg
     2701 tcggatagag aaatcgaaga cctgctgaaa atgatgcgat tggaaaatac agggaaaaag
     2761 aaagcaaaga atttctcttt aggtatgcgc cagcgtttag ctattgcaaa tgcactgatc
     2821 acgaatccag aatttttgat tttggatgaa ccgacgaatg ggatggatcc agcagggatg
     2881 gcagaaatgc gagaaatcat ccaacgtttg gtaaaagaac ggggcatcac cgtcttgctt
     2941 tctagccact tgctagatga attgtcacag atcgccactc actacggtat tttgcatgaa
     3001 ggacacttga tcaaagaatt atcgaaagaa gaattggcac aagaatctcg tcaatttata
     3061 aaaattgaca cttcggcgac ggaacaagca gtaacggttc tcgactctct tggttatagg
     3121 gattattttg tccaatctag ccgtgttatc cagctttttg aaggaattga ccaagtagca
     3181 gccatcaatc aagcattggt agaggcaaaa gttcctgtgg atggtatcca cctagttggg
     3241 caaaaactgg aagattattt cttacaatta actggaggca atccacatgt ttaatctatt
     3301 aactgctgaa aaaataaaag tatggaagag ccgtaaaatg tgggtctcac taggactgat
     3361 ggtcgtactt ccgctttttt attcttggaa cgaatggtat atgcatacaa aatacggcaa
     3421 tgaattaaac caagcaacgg atacagtcat caacggtgca acagggattt taatggtgga
     3481 aaaaatggct gcatggattc ttctggcttt cgcagcattt gcgtgctttt atattgggga
     3541 agaatttcaa aacggtacga ttcgcaatac gttatcttta ggacggaatc gaatgactta
     3601 ttatgtgtcg aaacttctcg ttacgctttt gatcacaaca cttggcgtgg ttttgattac
     3661 ggggctagcc atgattgctt acacacttgc tttcggattt ggtgaagttg agggaatcaa
     3721 aaactatgga aattatgttc taaaagtatt tcctgtattg cttctgctga tattagcgac
     3781 attatctgtt ccagtggctt tgacattcat cactagaagt accagtgtca gtttgcttct
     3841 ttcttttctt tatatcatgg gaacggcctt cgttcccggt gttttcgcaa aaatcaaagg
     3901 cttggaattt ctaacggagt ggtttacgga aacgtggctc atgtatacgg actttgcgca
     3961 acaagcaact tattcgcaag caccgaaaat gattctggtc agtctagtaa caatcgtggt
     4021 atcatcagct ctaggaatgt ttattttcca aaaatctgac attaagtagg tgttcctgct
     4081 ggaaactatt ttgattatcg aagacgatga agctgttcat tctttactgg aggaggttct
     4141 tgaacagaga tacaaacttc ttgatgctta ttcaggtacg gaaggaaaat tattgttaag
     4201 cacatatcct gtcgatttga tccttctaga tctcatgctt ccagggcttt caggtgaagc
     4261 attgttagca gaaatccgac agacatcgaa tgttccgatc atcgttcttt cagcaaaaag
     4321 cgatcaaaga gataaagttt ccttgttggc agcaggagcg gatgactatg tgacgaaacc
     4381 atttgatatc gaagaactgt tgttacgaat cggcatccaa cttcgtcatc agacttctgt
     4441 ccaagtaaaa gatctgtcgc aacgcatcta ttacaaggaa atcttactgg acaaagagtc
     4501 gagggatgta aatgtgaatg gtgtggctgt ccatttgaca ggaagagaat tcgatctgct
     4561 gaagcttttt ttagaacatc cgaaaaaagt attttcgcga gcaaatcttt atgaaacagt
     4621 ttggcaagaa ccatattttg atagtgagaa aacagtgaat atgcatattt ccaatcttcg
     4681 agggaaacta gcaaaggcac atgctgtgta tattcatacg gtgtggggag tggggtttag
     4741 atttgattaa gtggctgttg cttctcgttg ttatctttat gggaagtttt attttcttcc
     4801 tgcaaaggga aatcaagaga ttaactaaga aaatcgccac tctttctgaa cgacaagaat
     4861 atggtggtcg tctttcagtc gaatttcgag aaaagaatct aatggaccta gtagatgcac
     4921 tgaatacatg ggtggatgaa aatgaacaaa aaggacaagc atttcaagaa atggaagaaa
     4981 acatcagact atcgatcgtc ggcctttctc atgatctcag aactcctttg actgctatta
     5041 acggttatgt acaattactg gaacaggtag aggatacgga gaaacgggag aagtatttaa
     5101 cgatcatcaa acaatctgtt gcaagattac tcagtatgac ggatcagttt tatgatcttg
     5161 ctcgaataga gacgaagcaa aaagaaatgg atctccatac gctttctttt tctaaagagg
     5221 tggaagaagt atttttctct ttttatgaac aattttctga gaaaaatatc gaggtagcgt
     5281 tcgctgaaca ctcgataaat acaactgtat tagcagatcc gctcatgttg ctgcgagtgc
     5341 tgcaaaatat catacagaat ttattgcgtt atgcagatgg acaagcaaag gtgacatttt
     5401 caaaagagga ttcattcatt cgattaacag tggccaatcc tatcaaacca ggagcgcaaa
     5461 cctctgttga aaaagtcttc cagcgttttt ataccgaaaa taccagccgc acaaatacgg
     5521 aatctagcgg aatcgggctt tacctgtcaa aaaaattggt ggaaggaatg ggaggagaga
     5581 tgaccgctag attagacggg ggcatcttct ccatctccgt caagttgcgg agggtttaat
     5641 tggctctaat ttacctgaag tgaatgacaa aaaattgtat ctcgttttaa agacatcttc
     5701 ttttttggaa ggtgttttta ttttggtgtg attgatacga attaggtaac gaagacagtt
     5761 atttttgtac ccccttttca aatagcgcaa cagtgctata ctaaaactaa caaatcaagg
     5821 aacggagagg gatatgggcc aaagagaatt tatcgtttta attattattg cagttgttat
     5881 tttattagtt gttttagaca ttttttcacg tttgaaagta aaggaaacag ttcgtagtaa
     5941 ttggggaaaa attccttatc aaccgagatt tgataaagaa gaaagtttga aggatgcgtg
     6001 gctcacggag aaaaaattcc gttcatggga tagtgaaatc gacgatctta cttggtatga
     6061 cttggatatg ttcgaagtat ttgaagggat caacagtacc tattctagtg taggttcaga
     6121 agcgttgtat cagcgtttac gtagctttga ttttggagaa gattatcagt tagaaaaact
     6181 aattgctttt tatcaggaaa atcctcaact gagagaaaga atccaatatc aattcgctcg
     6241 tttaggaaaa aaagatcata attttgcaaa acaatatcta gcggatggaa aaagtaaaaa
     6301 gattggacgc gtaggtttat ttatttttct tggcttgctt ccgctggtgg gtgtaggttt
     6361 gcttctttta gggcaagtac taggaatcta tctagcaatc ggtggcttag tattcaatac
     6421 ggtctattat ctagtgaaga aacaaacact agaaacagag ctgaatagca tgagttattt
     6481 ggtttcaacg atttcagccg ccaatcagtt agcaaaaatg acgacaccac ttcaaaaaga
     6541 gctgaaacag caaactaaac cattaagaga catccctaaa ttaggttttt catttcgaat
     6601 gaagagcggg agtgaagcag aattgatttt tgaatatgtc aacatcatgt tcatgctgcc
     6661 atttatctcc tatcatttcg ttctcactaa attagaaaag cattcaactg aagccatcaa
     6721 gctgtgggaa ttgctgggag aattggaagt tgcagcagcc gttttgaatt atcgcaccta
     6781 tatgccgatc acttgccgac cagagtttac tgagggcggt gtgacagcga aagatatcta
     6841 tcatccattg ttaaaagaag cggtagttaa tccagtcaac tggcagaaga atacattagt
     6901 cacgggttcc aatgcatcag gaaaatctac gtatgtcaaa agtatagcca tcagctgtat
     6961 tctagcacag acgatccaaa cagcgattgc agaacagttt accctgcaac ctggacatgt
     7021 gttgacttcc atggcaatcg aagatgattt atttgaaggc gacagttatt ttgtttcaga
     7081 aatcaaatcg atcaaacgtt tgctggacca agtggcttcc aaagaacgct gctactgctt
     7141 cgtagatgag attttaaaag gaacaaatac gatcgaacgg attgcttctt ccgcttcagt
     7201 cgttcggtgg cttgcggagt atccatcttt agcatttgtc gccacacatg atatcgaatt
     7261 gacagagatt ttaaagaatt attgtgagaa tgtccacttt gaagaacaag taacagaagg
     7321 aaaaggaatc agttttgatt tcaaattaaa agaaggacct tcaaggaccc gaaatgcgat
     7381 tgccttgctg aaagttttga attatcctaa atccatagtt gaacaggccc aaaaagaaag
     7441 tttgttgttc gatgaacagc gtcagtggta tccgtttgat taactttatc aaacgaagca
     7501 tgatgcgtgg gtccaaaaag acgattatca aaagaagtac attgatgcca aaaaagtggc
     7561 agaattcaat gggaacgagt ctgctgtcca gatgatcttg gaagatttag caaaagacga
     7621 tacaaaataa accacgctgt cttccttcta gcagctttta tcgaaaaaac ttagcatcag
     7681 cgacgagacg cacgttccat ggcaaagttc tccgtaaaac aaacagcgaa aacagaaatt
     7741 gaaaataact atttccattt tatttatttc ggttaaaata aaagaaaaaa gctttcgata
     7801 aaaggacgga ggaaagctta acatgaataa aaaagacaag aacctcagtt ataaggatct
     7861 tctatatgtc atcgtagtag ttgctgtcgg tacttttatg attttaaaag ttccagagtt
     7921 gtttagatag ttattctatc gaagatgatc atgaactaga aaacacatct cctctttgtt
     7981 agcaggtgtg tttttgtgtg gaagccaagt tgttttttca tctgctgtat tccatgatac
     8041 aattaagaat catgtctata aggagatagg aaataaatgg caaatgcgct tgaaataaat
     8101 aatttgaaaa agatctatgg aagtggggtc gaagcattgc ggggaatcga tctttcggtg
     8161 aaagaaggag atttctatgc gctattaggg ccaaatggag ctgggaagtc tacaacgatc
     8221 gggattatta cttctttagt caataaaaca tctggaaaag tgaatgtctt cggttatgat
     8281 ctggacaagg atctagttcg agcaaagcaa cagattggat tggttccaca agagttcaat
     8341 tttaaccctt ttgaaacggt ccagcaaatc gtcgtgaatc aagcaggcta ctacggtgtc
     8401 tctcgcaaag aagcgatgaa acgcagcgaa aaatatttga agcagtccaa tctttgggaa
     8461 aagagaaatg tccgtgcacg tatgctttcc gggggaatga agcgaagact gatgattgct
     8521 agggcattga tgcatgaacc acgtttgctg atcttggatg aaccaacagc aggagttgac
     8581 atcgagttga gacgcgagat gtgggagttt ctacgagaat tgaacgaaaa tggtacaacg
     8641 atcattttga cgacgcatta tctagaagaa gctgaaatgc tttgtcgcaa catcggaatc
     8701 atccaatcgg gtgaactgat cgagaataca agtatgaaag agcttctgtc aaaactgcaa
     8761 tttgaaacat ttatctttga tttagcgcca tatgatacca aacctgtgat tgaaggatat
     8821 gcttgtacat ttgaagatga gcagacaatc gctgtcgaag ttgaacgaaa tcaaggggtg
     8881 aacggaatct tcgatcagct tacaagacaa ggaatcaaag ttctttctat gaggaataag
     8941 tcgaatcgtt tagaagaact gttcttgaaa atcacagaag aaaataatca tacggaggag
     9001 aaaaatgttt aatctttatt ttactgcatt aaaaagttta gctatcaagg aaacgaaccg
     9061 ctatttgcgt atatgggtcc aaacactggt tccgccagtg atcacgacat ccttgtattt
     9121 tgttattttc ggaaaaatga tcggtgggcg gatcggtgac atgggtggtt tttcatatat
     9181 ggaattcatc gttcctggtt tgattatgat gtctgctatc actagttcgt atgccaatgt
     9241 ttcttcgtcc ttcttctctc aaaaatttca gaaaaatatc gaagaaatct tggtagcacc
     9301 tgttcctacg catgtgatta tttggggctt tgtgattggt ggtgtgggtc gcagtattct
     9361 cgtcggctct ctcgtaacga tcatttcctt gttctttgta ccacttcatg tgtattcatg
     9421 gttcatggtc atcattacat tattgatgac ggcaatcttg ttttcattag caggactgct
     9481 caacgggatt tttgctcagt cttatgatga tgtatcgatc gtgcctacat ttgtcttgca
     9541 gccgttaact tatctaggtg gcgttttcta tgccatctct atgttaccgc ccttttggca
     9601 agctgtttca aaagtcaatc cgatcgttta tatgatctcc ggtttccgtt acggattcct
     9661 tggaacgatc gatgtgccag tagcaacatc gatgatcgta cttgtgttat tcatcgttgt
     9721 tttgtacgcc gttacttggt atttgatcaa taaaggaaga ggattgagaa gttagaaaag
     9781 aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacgggaa aatagttcct
     9841 catattttgt tgttttttga gcgaataccg agggtcagct gattgaacac cgtttagtgg
     9901 agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     9961 ctcctatttt tcgtttggtt ggacttatta ccgcaaatgc aagcttttga acaccattta
    10021 ttccggaaaa tggactgtga taagattttt gtcacagtct tttttttgct caatcagaaa
    10081 catcttttaa gctgttcact ttgcttcttt ttttgtttcc tagtataatg acaatgtttt
    10141 tgagaaagat tgcgacttca ctgcttgata gaatggagag taacaaataa atgactacta
    10201 atttttggaa agagctgcca aagccttttt ttgttttagc accaatggaa gatgtaacag
    10261 atgttgtatt tcgccatgtc gtaaagcatg cagcagcacc ggatgtcttc tttactgagt
    10321 ttacaaactc agacagtttc tgtcatccag atggaaaaga cagtgtacga ggaagattaa
    10381 catttacaga agatgaacag ccgatggttg cccatatttg gggagacaag ccagaatttt
    10441 ttagagaaat gagcattgct atggcagaaa tgggcttcca aggaatcgat atcaacatgg
    10501 gctgcccagt ccctaatgta gcagaacgtg gaaaaggcag tggcttgatc cttcgtccag
    10561 aagtagcggc acaattgatt gaagcagcta aagctggcgg tttgcctgtc agcgtcaaga
    10621 cgcgtatcgg ctatacggaa atgtctgaga tggaagaatg gatcagtcac ctacttcatc
    10681 aagatatagc caatctttct gtccatttgc gtacgcgtaa agagatgagt aaagtagatg
    10741 cacattggga agtcattcct aaaatcattg ctttacgtga ccaaatcgct ccacaaacac
    10801 taatcacgat caatggagat atccccgatc gccagacagg agaagaattg gccgagaaat
    10861 atggcgttga tggcatcatg attggccgag ggattttcaa gaatccttat gcatttgaaa
    10921 aagacccgag ggaacataca gaaaaagaac tgatcggact acttcgtctg caactagatc
    10981 tgcaagatca ttattcagaa atcatcccgc gttccatcac aggactacat cgtttcttta
    11041 aaatctatat caaaggattt ccaggagcaa atgacttgcg agttcagttg atgaatacga
    11101 aatctactga tgaagtacga gagatcttag agacctttga aaaagaacat ccaacgcttt
    11161 tcagtgaaca atgatttctt gcttgaacac cgtgaattag aggtcgtgaa aaagctgtcc
    11221 tgtatcaagt aataagaaca gccaaacaaa aatagctctt catattttgt cgttttttga
    11281 gctaataccg agggtcagtt gattgaacac cgtttatatc agaggttgtg agcctgccgt
    11341 ttaactctga gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg atgaatattt
    11401 tgacttgtga ccgaagagtt ggtaggagaa caccgtttat aagaggttgt gacaaaagtc
    11461 ttgtcgcaac ctctttttta accaaaaaag tgcaaatagt ttactttaag taagagaata
    11521 attatgatga ataaaaaagg tggaacaaat agcatactat atatattgaa atgaacactg
    11581 tatggatgct ccaacttgat gaagctaagg aggaattcgt atgtcagaaa aggaattaat
    11641 gaaggtagtc ggtttttatg aagggttgcc gatcgatgat ccaaaaagct ttattgatga
    11701 aaaagaacag atacctgtac ccagttcacg ggatcttctg gtaaaagtca acgcagtatc
    11761 tgttaatcct gtcgatacga aacttcgcca agataacggg ataagaaatg ctttacgtat
    11821 cttaggtttt gatggagtcg ggaaagttgt tgctgtaggt gacgaagtgc aaaaattttc
    11881 tgtcggcgat cgcgtttttt acgcaggtac aacaacaaga gcaggaagca atcaagaata
    11941 ccaactagtt gacgaacgta ttgtcgctct tgcaccaaaa aatctttcag atgaagaagc
    12001 tgcagccttg ccgttgacct cattgaccgc ttatgaattg ttatttgaaa aatttggtct
    12061 aactccagaa gaaaatgcca atcgcgggaa aaaaatctta gtgatcaacg gatccggggg
    12121 cgtcggttcg attttgaatc aactagcgca ttgggctggc ctagaagtct acgcgactgc
    12181 aagtccgaag aattttgaat ggctgaaaaa aacaggtgta gactatccaa tcgattatca
    12241 tcacgactta agtaaaagtt ttcgtgaatt aggaatcgat caagtagagt atgtcgctgt
    12301 attattcgat attacccgct actttgatca aatcaagcac cttgtccgac catttggaca
    12361 tgtgggaacg atcgtaggga tcaaagagcc gttagatata ggtgtttgga aaaaccaatc
    12421 tatcagcttc gactgggagt atatgttcgc taaaacagac tataactatg aaatcgaaac
    12481 gcaaggagaa gctttagcgc acattgctgc tttagcaaat gaagggatta tccgcagtac
    12541 attgagcaag gtctattcag atgggatgaa tgcgaagaac ttgaaacaag caaccaaaga
    12601 cgtagaaaca ggacatatgt taggaaaagt cgttgttagt ggtccgttca acggataagc
    12661 aagaatacgc cgattttttt cttatttgac tttgataaaa acaagagcgc atgacagtaa
    12721 tttgccataa gctcttgttt ttttatttat aataagcgaa tcaacgaaag ggaaagaatt
    12781 cctgccaaga cgatcaccaa taagcttttt gaaagaatag cagctaaaag tgtaggaact
    12841 gaagccaaag cattttcgag attgatctgc ggcagatgtc cgatattttg ggtaaataga
    12901 ctgctgaacc ataaagcaga cataatgacg atcgggacaa aacttaaata ctcaagcaac
    12961 ggttgcggta aatcgaattt tttcagtaag acaaatggta ggaccctaga aagccaagtt
    13021 gctaaagtac aacctaaaat agtcagcaat gtaaactcaa aagaaggcat gtttgatcca
    13081 cacccccaat ccgcacccaa tcagtgttac aactacaatc actaagttgc ttgggataaa
    13141 aatcagtccg acatatacca gaatcaaagt gagaccaatc aggatcaact gtaatcgttt
    13201 agaggtgttt ctatcagaaa tgacttgaag ataaagtaac ccgataaaca tagcgacgat
    13261 ggcgaaatcc aaaccaaact ttttcggatc agtaataaaa ttccctaaaa gtgcgccaac
    13321 aagagaagaa cccacccacg tcaaataaga aatccaatta gctgtattca tccaacgaaa
    13381 gtttaacttt tgtccagtat agttcaattt attcattccc agtgcgaaac tttcatctgt
    13441 cagcaatgtg ccaatcagca gattttgaag cagccgattt ttcttgaaat aaggagcaat
    13501 cgtcatactc atcagaatca ttcgggaatt gactagaaag gtcgagaaaa cgatggaaag
    13561 caatgggctg tgactagcaa gcatgctgac tgtgataaat tgggcagaac cagcatagac
    13621 cagcagggac atcaacgtga cgactagtgg atgaaatccg gcagctttcc caacgatacc
    13681 aaatgcaagt ccaataccga tataaccgaa aacggtaggt aacgtgtctt tgatcgcagt
    13741 ttttatatct aacttttcat ccaataacaa aactccttta gcacgacata aattttcata
    13801 attgtcgtta aacttgtgaa tattctaaat ttttaatgag catttgtcaa tggtgggatt
    13861 gaaacgtaaa gaactagcgg gtcgcagatt tacacgtatt gtttcggtgg gaggaggcgc
    13921 gaaaaatctt gcatggctac aaatccaagc tgatattttc gatgcaacgg tctattgttt
    13981 aagtatggag gaaggacctg cattaggcgc aacaatgatt gctgcactag ggttagggtg
    14041 gttcgacaca cctgaaagtt gttgtcagaa ttttatccaa tatacagatg cagcggaacc
    14101 tctcttacaa aatgtagaaa agtatcataa aatctatcaa caatgccgga aaatctatcc
    14161 agcaacgaag gaaatttgtc atgacttata aaacaaaacg cgtacctaat agtcaaatga
    14221 ctaatattgg gtacgcgtta agtcttcttt acttcatcct ttgcatgatt cgtgttttta
    14281 aagcaaggtc tgctgtattt cttgtggatt taggatggtt gttatcgttt acttgctatt
    14341 tttcattgaa atcgtctttc tttaccgttt ctttagtttt ttatctattt ttttcttttg
    14401 gttagaaaat tctttttccc gaaagtcttc tttcaggatt tttatagagt agcgttttga
    14461 ataagaggcg tcagtatgat cgacttggca gacgaaccaa gagaacattt gccttttttc
    14521 tgcatgaggt gcttctgcta ataattcttt ccataaataa taacagtcct ctatcagaag
    14581 aagggttcgt tcatcctcag gctcccaata gaatgtttcg agagtagcaa tcacttcatt
    14641 gatcaattcg aatgcgggaa gataggcatt ttgctgaata agcgattcaa ctgcttcttg
    14701 gatgaacttt cgcatctcca tgatgaactt cgctgttttc ctgtttttgt ttttcttgat
    14761 aatatgatcg tatttatgga tgatagttgt taatttatct gaataattta ttgcatgaaa
    14821 tggcatctta actttgaagc gttcgataaa gtgcggatgt tctataagaa tctctgttaa
    14881 aagctcccgt acaagagagg aactagcgga ggaaacaagg gacgagagtt cggaaagtga
    14941 gtagttggat ttttttattg gacgttcttc gtaagcgaat aagacagcag ccatgtgttt
    15001 gcattttttt ccttctatgg catatagaca atcacaagtc atgttggtct tttcatccca
    15061 agtgatcact accttataat cttccgtgcc agaaactgtt gcggacaagt acttgcttgt
    15121 atagttcaaa tgacggacaa aaccgtgcag atgatagctg taaccgcgtt ccaaaacgtt
    15181 ttcaggaaaa tactcaagcc aattattcat ctagttctcc tttcatgaaa cacttattta
    15241 tatttagtat aaagaaacgt ttaagaaaaa agtagctgat tttacatatt tgataatcac
    15301 ttttgctcga attgtcaaaa attcattctc cttttctttt cttagagaat gtg
//
LOCUS       DAGCWU010000056        15232 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.056, whole genome shotgun sequence.
ACCESSION   DAGCWU010000056 DAGCWU010000000
VERSION     DAGCWU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.056"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(21..251)
                     /locus_tag="MBZ71_001383"
     CDS             complement(21..251)
                     /locus_tag="MBZ71_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429344.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="MBZ71_001384"
     CDS             complement(687..1253)
                     /locus_tag="MBZ71_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4429345.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="MBZ71_001385"
     CDS             complement(1253..2209)
                     /locus_tag="MBZ71_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBT4429346.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="MBZ71_001386"
     CDS             complement(2209..2865)
                     /locus_tag="MBZ71_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBT4429347.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="MBZ71_001387"
     CDS             3235..5253
                     /locus_tag="MBZ71_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4429348.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="MBZ71_001388"
     CDS             5680..8094
                     /locus_tag="MBZ71_001388"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBT4429349.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="MBZ71_001389"
     CDS             8442..9995
                     /locus_tag="MBZ71_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBT4429350.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="MBZ71_001390"
     CDS             complement(10376..12160)
                     /locus_tag="MBZ71_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429351.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="MBZ71_001391"
     CDS             complement(12175..13896)
                     /locus_tag="MBZ71_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429352.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="MBZ71_001392"
     CDS             13996..14757
                     /locus_tag="MBZ71_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4429353.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4956 a         2791 c         2995 g         4490 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga ta
//
LOCUS       DAGCWU010000057        14904 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.057, whole genome shotgun sequence.
ACCESSION   DAGCWU010000057 DAGCWU010000000
VERSION     DAGCWU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.057"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..483
                     /locus_tag="MBZ71_001393"
     CDS             <1..483
                     /locus_tag="MBZ71_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4429354.1"
                     /translation="PLLFSTITRDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRH
                     VWADLMDELEHQRHTALNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTM
                     LTFAAMNLKKLATWLWKAKKPMAFYLKFLQRLTKRSWQLSHSFLSTVWELYFDYSFFF
                     "
     gene            572..958
                     /locus_tag="MBZ71_001394"
     CDS             572..958
                     /locus_tag="MBZ71_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429355.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINDEKRSVLSHEKKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            945..1643
                     /locus_tag="MBZ71_001395"
     CDS             945..1643
                     /locus_tag="MBZ71_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4429356.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            1648..2112
                     /locus_tag="MBZ71_001396"
     CDS             1648..2112
                     /locus_tag="MBZ71_001396"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBT4429357.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLVLAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICILIYIILDEKDQRKK"
     gene            2109..3020
                     /locus_tag="MBZ71_001397"
     CDS             2109..3020
                     /locus_tag="MBZ71_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBT4429358.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKPAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            3255..3794
                     /gene="pyrR"
                     /locus_tag="MBZ71_001398"
     CDS             3255..3794
                     /gene="pyrR"
                     /locus_tag="MBZ71_001398"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBT4429359.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            3808..5091
                     /locus_tag="MBZ71_001399"
     CDS             3808..5091
                     /locus_tag="MBZ71_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBT4429360.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            5104..6030
                     /locus_tag="MBZ71_001400"
     CDS             5104..6030
                     /locus_tag="MBZ71_001400"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBT4429361.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            6049..7329
                     /locus_tag="MBZ71_001401"
     CDS             6049..7329
                     /locus_tag="MBZ71_001401"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBT4429362.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            7334..8410
                     /gene="carA"
                     /locus_tag="MBZ71_001402"
     CDS             7334..8410
                     /gene="carA"
                     /locus_tag="MBZ71_001402"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBT4429363.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            8415..11600
                     /gene="carB"
                     /locus_tag="MBZ71_001403"
     CDS             8415..11600
                     /gene="carB"
                     /locus_tag="MBZ71_001403"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBT4429364.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTYHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            11654..12430
                     /locus_tag="MBZ71_001404"
     CDS             11654..12430
                     /locus_tag="MBZ71_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBT4429365.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            12427..13359
                     /locus_tag="MBZ71_001405"
     CDS             12427..13359
                     /locus_tag="MBZ71_001405"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBT4429366.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDPDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            13356..14072
                     /gene="pyrF"
                     /locus_tag="MBZ71_001406"
     CDS             13356..14072
                     /gene="pyrF"
                     /locus_tag="MBZ71_001406"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBT4429367.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKEIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            14079..14708
                     /locus_tag="MBZ71_001407"
     CDS             14079..14708
                     /locus_tag="MBZ71_001407"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBT4429368.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            14696..>14904
                     /locus_tag="MBZ71_001408"
     CDS             14696..>14904
                     /locus_tag="MBZ71_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429369.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISS"
BASE COUNT         4810 a         2665 c         3396 g         4033 t
ORIGIN      
        1 ccgttgttat tttcaactat cactcgtgat ggttatcgaa cctataaatc aaatccaaaa
       61 ttctgtagga gttgtccatt acttaaaaac tgtactttga gtacaaaaca tcaaaaaata
      121 gtaactcgac atgtttgggc ggatcttatg gatgaacttg aacaccaacg ccatacggcg
      181 ttgaatagag aaatttataa aaagagaaaa caaaccattg aacgaatatt tgcagatgca
      241 aaagaaaagc atggtatgcg ttggacgaaa tatcgaggtc tcgaaaaagt cgccacgcac
      301 acgatgctta cgtttgctgc catgaattta aaaaaactag ccacatggct atggaaagca
      361 aaaaagccta tggcttttta tttaaaattt ctacaaagac taacaaaaag gagttggcaa
      421 ttaagccact cctttttgtc tacagtctgg gagctgtatt ttgactacag cttctttttt
      481 tagacgatag ggaattaatt ttgtttacac taggatcatt ctttttcttt attgtatttt
      541 gagctatact aaagcataat aggagggtat gatgaaaacg acatttataa gaaaaaccgg
      601 tttttacggt atgggtagct cgattcagct aagaatcaac gatgaaaaac gcagtgttct
      661 cagtcatgaa aaaaagataa cgcttgatct ggaagtgccc tttacgatgc aggtcacctt
      721 ttattggcta aaaagccccg tttatacagt gaaagaagca aagcctgctt atgtgatcac
      781 aatgaattac ttgattcttc aaatctatcc gtttttattt ctagcaactg ggttgggggc
      841 cgttttccta caaaatttat ttttcagttt ttttgcagtt attgtgttga ttgcgttctt
      901 tttctatata aaaaaccggg catatttgat caaggaggaa aattatggca aactctgatc
      961 tttttttaat cagtttcaat cgaatcgaaa aatggatgag agacgagatg ggaaatgcac
     1021 gaaatatggg atttaccgaa cttgtcagac gcctagctca aagaaaacaa ttaatgatca
     1081 gaaaatacga agatgatctt ttacagcttg ctcagttaag gaatgcgatc gtccatgatc
     1141 ggatcgcagt cgattttgtc attgcagagc caaatgattg gacagtcaag cgaatccagc
     1201 tgatcgaaca agaactgatt cagcctgaaa ccgtgctgcc tcgatttgca aaacgtgtaa
     1261 caggttttga acaagatctt ccgttacttg aactgttaaa gatagtagca gaaaaaaggt
     1321 attcacaatt tccgttatac aataaaggaa aatttgtagc attgattact ttacgtaata
     1381 tcgggttttg gctagcaaaa gaaagccaaa aaggtccggt tgatttaaca aataaacgag
     1441 cgttggattt gatcatccaa aacggaaagt acacaaatta tcattttgtt ccagctaaaa
     1501 cacatattta cgaggttgaa gcgatgtttc gtgagcaagg gactttggaa gccgtactta
     1561 tcactaaaga tgggaatccc gatggtaatc tgctcgggat cgtacgacct cgtgatattt
     1621 ataaacaaat agaaaaggat tgattttttg ttaattattt attggatatt cagcgctttg
     1681 ctagtaggag tggatcagtt ggtcaaatgg tggatcactg ataatctagc gcttggagag
     1741 acgaaaaatc ttataccagg tattttgtca ctgaatcata ttcgaaacac tggagccgca
     1801 tggagtatgc tagaagggaa gatgtggttc tttactatag tgacactcat tgcagtcgtt
     1861 gtgattctca cattgatgat taaaaatcgt gaaaaaggta atcgctggtt catgattgga
     1921 ttgagtttgg ttctagctgg tgcgattggc aattttatcg atcgtattcg tcttggctat
     1981 gtagtagata tgttccagac agattttatg agctttccga tttttaacgt agcagatgtg
     2041 acacttgtat gtggagtgat ttgtatatta atttatatta ttttagatga aaaggatcaa
     2101 agaaaaaaat gacagaactt aaaacgacga ttcaccatga atctggtagg atcgataaag
     2161 tattgactaa tttattcacc gattacagtc gttcgcaaat tcagctgtgg ctgaaaaatg
     2221 gagcggtttc tgtcaatgga cagccagtca aagcaaatta caaagtaaaa caaggagacg
     2281 agattactgt cttgatcccc gaaccagaaa cattagatgt ggtcccagaa aatatcccgt
     2341 tgacgatcgt ttacgaagat gaagatgtag cggtgataaa caaacctcaa ggaatggtcg
     2401 ttcatccttc agcaggtcat acgcatggca caatggtcaa tgcgctgatg taccatatca
     2461 aggatctttc tacgatcaac ggcgttattc gtccaggtat tgttcaccga atcgacaaag
     2521 atacttccgg actgctaatg gttgccaaaa atgaccaggc acacgaatca ttagctcagc
     2581 aattaaaaga taagacctct ttgcgcaaat atgttgcttt agtccatgga gagattcctc
     2641 acgaaaaagg aaccatcaat gcgccaatcg gccgttcgaa ggaaaaccgg aaaatgcagg
     2701 cgataagaga agatgggaag ccggctgtca cacattttac tgtattggaa cgtttcgaag
     2761 gatttactct ggttgaatta aaactagaaa ccggaagaac acaccagatc cgtgtgcata
     2821 tgaagtatat cggttatccg ttagctggag atccagtata cggaccgaag aaaactttga
     2881 aaggcaatgg tcagtttctc catgcgcaaa tgttaggttt tacgcacccg actacaaacg
     2941 aaaagatgat ctttgaagca cctcttccag aagtttttga aaaaacttta gaaaaattaa
     3001 gaaaaaacat tgcattttaa tttagataac ggtatagtaa ttaacaacga aagcataaaa
     3061 cttaatagaa ttcctttaag tttagtcccg tgaggctaag aagggtggga gcaaggatac
     3121 gtatggtgcg ccataagtag attgctcatt gacatagtat agctatcagt gtatcagtat
     3181 acgctcagtc taaacattcc tcctgtcctc ttagggcagg agttttttta tgcaaaaaag
     3241 tagggggaat gaatatgcaa gcaaaagaag tagtagatca ggtaacgatg aagcgagcat
     3301 tgactcgaat tacttacgag attatcgaga gaaatcatag tatccaagat attgtattag
     3361 taggtatcaa aacacgtgga atctacattg catcaagaat tgccgaacgc ctgaagcagc
     3421 tagaagatat cgatattcct gtaggagaac tggatatcac tttataccga gatgacaaaa
     3481 aagaaaatcc agaagaaccg gaacttcatt cttctgacat cccagtttcc ttagaaggaa
     3541 aagaagttat tttgattgat gatgttttgt atacaggacg aacgattcga gcagcaatgg
     3601 acgcagtaat ggattttggc agaccaagaa aaatttcatt agcagtcctg gtcgatcgtg
     3661 ggcatcgaga attgccaatc cgtgcagatt atgttgggaa gaacattcca acatctcggg
     3721 cggaagagat tttagtagag atgcaagagc ttgatggaca agatcgtatc atgattttga
     3781 aagaggaaga ttagaggaga gagaaacatg tcggagaaaa aaggatttca taatgatgac
     3841 gtggtgttag atatccacga tcgcccaacc ccaggacatt gggtaggatt aagtttacaa
     3901 cacttgttca cgatgttcgg agcaaccgtg ttagtcccca tcttagtagg tatcgatcca
     3961 ggtattgcgc tagtcagttc aggactaggt acattggtgt acttatttac tactaaagga
     4021 aagatacctg cgtacttagg aagcagcttc gctttcattg cagcgatgca gcttttgatg
     4081 aaaacagatg gttatccagc gattgcacaa ggagcagtca caactggatt agtctatttg
     4141 attgttgcgc tgatcatcaa aaagatcggt tcagcatggc tggataaaat cttgcctcca
     4201 atcgtcgtcg gtccagtcat catggttatc gggttgggac ttgcggcaaa tgcagcaaca
     4261 aatgctatgt ataatcaggg cacgtatgat ttcaagtata ttgctgtagc gctgatcaca
     4321 ttaggattaa caattttctt caacatgtgg ctaagaggtt ttctcggatt gattccgatt
     4381 ttactaggta tcattagcgg atacatcgta gccttgctgt ttggaatcgt ggatactaag
     4441 ccaatcgtag acgctgcctg gtttgctgtt cctaactttg agattccctt tgtacaatat
     4501 gaaccaaagc tatatcttgg agcaatcaca acaatggcac ccatcgcttt tgtgacgatg
     4561 acagagcata tcggccatct tatggtattg aataaactaa ctaaacgtaa tttctttgaa
     4621 gaaccgggtc ttcataagac gttgacaggt gatgggttag cacaaatcgt ggcagccttt
     4681 gtcggaggtc ctcctgtaac aagttacggt gaaaacattg gtgttctggc tatcacacgt
     4741 gtccatagcg tgtttgtgat cggtggagct gcagtatttg cagtcatttt aggattcgta
     4801 ggaaaattaa gcgctttgat tttaagtatc cctggaccag tcatctcagg aatcagtttt
     4861 atcttatttg gagtaattgc ggcaagtgga atgaagattt taatcgagaa caaaatcgat
     4921 tttgataaaa agaaaaatct gttgattgct tcagttatct tagtagtagg aattggcgga
     4981 ctcgtctttg aagtaggcac atttacattg tcggcaatgg cattagcaac tgtacttggc
     5041 atcattttaa atcttatctt accagaaact tcacgaagcg aagaaaacta aggaggaaac
     5101 attatgatca tcaaatcaga acgtatcagc ttaaagcatt tattgaccgt tgaggcactt
     5161 actgatcaag aagttatggg cttgatccgc agagggcagg aattcaagca aggtgctaat
     5221 tggactccgc aaaagcgcca gtactttgct acaaacctct ttttcgaaaa tagtactcgc
     5281 acacataaaa gcttcgatgt agcggagaaa aaactagggc tagaagtgat tgaatttgaa
     5341 gcaagcacaa gctctgtcca aaaaggggaa acactatacg atacagtact gacgatgtca
     5401 gcattaggcg tagacgtagc ggttatccgt catggtgatg agaactatta tgacgaactg
     5461 atacaaagta aaacaatcca gtgttcgatt atcaatggag gagacggaag cggacagcat
     5521 ccaacccaat gcttgcttga tttgatgacc atttatgaag aatttggtca ttttgaaggt
     5581 ctgaaagttg ccatcgtagg tgatatcact cactctcgcg tagctaaatc caatatgcag
     5641 atgttaaaac gtctaggcgc acaagtattc ttctcaggtc ctagagaatg gtacgacgat
     5701 gaattcgaag tgtacggaca ttatatgcca ttggatgaac tactagatca agttgatgtc
     5761 atgatgatgc ttcgggtgca acacgaacgc catgatggaa aagaaagttt ctcaaaagaa
     5821 ggctaccatc aggaacatgg gttaacagtt gagcgtgcga agaagttgca aaaacatgca
     5881 atcattatgc acccagcacc agttaaccgc gatgtagaat tagctgattc attagtggaa
     5941 ggattacagt cgcggatcgt tcagcaaatg agcaatggag tattcgtcag aatggcaatc
     6001 ttagaagcag tgctaaatgg aaaagcgtaa ttataggagg atgaaaaaat gaaaacactc
     6061 attaaaaacg gtcagatcaa tacacgaaaa aatatgacga caccagcaga aatttggata
     6121 gaagatgggc gaatcaaagc aatcggtaca ggattctcag aagccgaatt tgatgaagtg
     6181 tttgatgcaa agggacaatt gatcacacct ggtttagtgg acgtccatgt tcatttgaga
     6241 gagccaggat ttacatacaa ggagacgatc gaagctggga ctcgctcagc tgctcgtggc
     6301 ggtttcacta ctgtatgcgc gatgccgaat ttaaatcctg tacctgatac ggcagaaaag
     6361 ctgagacaag tatatgacat cattagaaaa gatgcagttg tgaaggtatt gcagtatgcg
     6421 ccaatcacag aaaatctgcg aagtgagaaa ttagttgatc aagaagcact gattgaagaa
     6481 ggtgcttttg ctttcacaaa tgacggtgta ggtgtccaaa cagctgggac gatgtatctg
     6541 gctatgaaag aagctgcaaa aaacaataaa gccctagtag cccatacaga agatgaatct
     6601 ttattatttg gcggcgttat gcacgctggc aagaaggcag aagaacttgg tcttcccggg
     6661 atcttaagcg taaccgagtc ttctcaaatt gctagagatc tgttgctagc tgaagcaact
     6721 ggtgttcatt accatgtttg ccatgtttct actaaagaaa gtgtccgtgt aatcagagac
     6781 gcaaaaaaag caggaatcca cgtaacagca gaagtttcac cccatcatct tattttgatc
     6841 gacgaagata ttcccgaaga tttcggcttt tggaaaatga acccgccgct aagaggacgg
     6901 gaggacagag aagctttgat cgaaggcttg cttgacggta cgatcgattg catcgctaca
     6961 gaccatgctc cccatggatt agaagaaaaa agccaaagtt tcatgaaatc accatttggt
     7021 atcgtaggaa gcgaaacagc gtttcaactg atctatactc attttgtaga aactggtcga
     7081 ttcacgttgg aacaagtgat caattggttg gcagttaaac cggctgagat ttttggtttg
     7141 aatgcaggaa cgctaacggt tggtgccccc gctgatgtag cagtttttga tattacacaa
     7201 acttgcacga tcgataaaga agacttttta tcaaaagggg agaatacacc ttttatcggc
     7261 tggaaagtaa aaggcgaaac acaaatgact tttgtcaacg gaaaacttgt gtggcagaag
     7321 ggagaataaa agtatggaac gtttgcttat attagaagat ggaacagttt tcgaaggaaa
     7381 ggcttttggt gcagatgcta gtgtcttcgg agaaatcgtt tttacaacaa gtatgactgg
     7441 ttatcaagaa actatcactg atcaaagttt taacggtcaa atcattacat ttacctatcc
     7501 tatggtgggt aattatgggg taaatagaga tgattatgaa tcgatttctc caacatgcaa
     7561 aggagtagta gtaaaagaac atgcacgtgt agcttcaaac tggcgaaacc aaatgacgct
     7621 agattcattt ttgaaacaaa aaggaattcc tggaatatca ggtatcgata cgcgcgccct
     7681 tactcgcaaa atccgtgaag tcggaacgat gaaagctagt atcgttgatg ctggagattc
     7741 cttcgaacat gcatttgatc agctgaaagc aaccgttcta gcaaccaatc aagtgcagca
     7801 agtctcaact aacaaacctt atccaagtcc aggaacaggt cggaaagtca ttgtcattga
     7861 cttcggcttg aaacatagta ttttacgaga attgagtaaa agaaactgta acctcacagt
     7921 acttccctat aacacagatg ccgagacgat tttagcgcta tcaccagacg gagtcatgct
     7981 gacgaatgga cctggtgatc caaaagatgt gccagaagca atcgaaatga tcaaaaaaat
     8041 ccaaggaaaa gtaccgattt ttggtatttg tctcggtcac cagttatttg ctttagccaa
     8101 cggtgcagat acctataaaa tgaaattcgg acatcgagga ttaaatcacc cggtacgaga
     8161 agtagcgacc ggaagaattg attttacttc tcaaaatcat ggctatgcag tcgatgaagc
     8221 cacgattgat ccagaaaaac tcatcgtaac tcatgtagaa gtaaatgacg gaacagtaga
     8281 aggagtcaaa cacagagatt atcccgcttt cagtgtgcag tttcatccag atgctgcccc
     8341 tggtccgcat gatgcactac atttatttga tgaatttatg gaaatgatgg acgcaggaaa
     8401 ggagcagtaa acagatgccg aaacgtacag atatcaagaa aattatggtg attggatctg
     8461 gtccgatcat cattggtcaa gccgcagaat ttgactacgc aggaacacaa gcctgccttg
     8521 ccttaaaaga agaaggatat gaagtggttt tagtaaattc taatcctgcg acgatcatga
     8581 cggataaaga agtggcagat caagtatata tcgaaccgat cacacttgaa tttgtatcaa
     8641 ggattcttcg aaaagagcgt ccagatgctc tccttccaac tttaggcgga cagacaggcc
     8701 tcaatatggc aatggagctg gcagcatcag gtattttaga agaattgaac atagaacttc
     8761 taggaacaaa gctttctgcg atcgatcaag cagaagatcg tgatctattc aaacagttga
     8821 tgaaagaatt gaatcagcca atccccgagt ctgaaatcgt caatacagta gaagaagctg
     8881 tagattttgc aaataaaatc ggctatcctg tcatcgttcg tccagccttt actttaggcg
     8941 gaactggtgg aggtatgtgt gaaaatgaag aagaattacg tatcgtatca gaaaatggat
     9001 taagtctttc ccccgctacc caatgtttga ttgaacgaag tattgcaggt tttaaagaaa
     9061 ttgaatatga agtgatgaga gattcggctg ataatgcaat cgttgtctgc aatatggaaa
     9121 attttgaccc ggtgggtatc catacaggtg attctatcgt atttgcccct agtcaaacat
     9181 tgtctgacca tgaatatcaa atgcttcgtg atgcttctct ttcgattatt cgagcactta
     9241 agatcgaggg aggctgtaac gtccaactcg ctcttgatcc acatagtttt aattattatg
     9301 tgattgaagt caatccgcgt gtttctcgtt catctgcgtt agcaagtaag gcgactggct
     9361 atccgatcgc taagctagct gctaaaatag cgcttggatt aacattagac gaaatgaaaa
     9421 atcctgttac aggaacgact tttgcagaat ttgaacctgc attagattat gttgtagcta
     9481 aaattcctcg ctggccgttt gataagtttg aaaacggaga acggctttta ggcacgcaga
     9541 tgaaagcaac tggtgaagtg atggctatcg gtcggaatat cgaagagtct ttattgaaag
     9601 ctgtgcgttc tttagaaatc ggtacatacc atatcgaact tcctgagttg gcagacatca
     9661 cggacgatga cctaatccaa aaagtggttc gagcacaaga tgatcgcctg ttttatgtag
     9721 ctgaagctat tagaagaggg tacaccatag aagaattagc ggatttgaca aaaatcgatt
     9781 tattcttttt agataaactg ttgcatatcg tagaaatcga acagcagttg aaacagcagc
     9841 cgttggatga caagatattg aaaacagcga aacaaaacgg ttttacagac aaaaaagtgg
     9901 cggagctgtg ggaaataaac gaagaagccg ttcgccagat gagaaaagaa aatgatttga
     9961 ttcctgtata taagatggtt gacacttgtg cagcggaatt cgaatcaaaa acaccttatt
    10021 tttatagttc ctatgaatat gaaaatgaaa gttcacgatc agaaaagccc tcaattctag
    10081 tactgggatc tggtcctatc cgtatcggac aaggggttga atttgactat gcgactgtgc
    10141 attctgtcaa agcaatccag caggcaggat acgaagcaat catcatgaac agcaatcctg
    10201 aaacagtttc gactgacttt tctatttcgg ataaattata ttttgagcca ttgaccttcg
    10261 aagatgtgat gaatgtgatc gagctagaac agccgatagg agtaatcgtt caatttggtg
    10321 gtcagacagc aatcaactta gccgaacctt tgactgctgc tggtgtaaaa atcttaggga
    10381 caacgatcga agacttggat cgtgcggaaa atcgtgattt attcgagcaa gcgctgaaag
    10441 agttggatat tcctcaacct ttaggggata ctgctacaag taaagaagaa gccgtggtta
    10501 tcgcttcgag aattggttat ccagttcttg ttcgtccaag ctatgtatta ggcggacgtg
    10561 cgatggaaat cgttgaaaac cagcgagatt tagaagatta catggaacat gctgtgaaag
    10621 catctccaga acatccagta ctagtggacc gttacttgct tggtggtgaa tgcgaagtgg
    10681 atgcgatttg tgacggtgaa acagtactca tcccagggat catggagcat attgagcgtg
    10741 ctggtgtcca ttcaggggat tcaatggctg tgtatccacc tcaaacatta tctgaatcga
    10801 tcaagaaaac gatcgaagat tacacgatcc gcttagcatt aggtttgaat tgtgtcggga
    10861 tgatgaatat ccagtttgtc atccatgaaa atgaggttta tgtgatcgaa gtcaatcctc
    10921 gagcaagtcg gactgttcca ttcttaagta aggtgacagg aattccaatg gcacaaattg
    10981 cgacgaaagc tattttggga gagaaactaa agacacttgg ttatgaaaac ggactttatc
    11041 cagaaacaaa agaagttcat gttaaagcac ctgtcttttc ttttacgaaa ttgcagaaag
    11101 ttgatactta cttagggcct gaaatgaaat cgactggaga agtaatggga tctgacagac
    11161 aattagacaa agctttgtac aaggcattcg aagcttctgg attacacttg cctgaatatg
    11221 gagccgtatt atttacgatt gcggacgaaa caaaagaaga agcactagaa ttagctaaac
    11281 gttttacaga aattggctat agtctgatag gaacgaaaca tacagctgct tatttcgaaa
    11341 aaaatggtct ggtcgttgaa acagtagcga aaatttctga ggaagcagca gaaaaaaatg
    11401 tagtcgactt aataagagat ggtaaaacac aagtggttgt caatacgatt gataaagatc
    11461 ggggaaatgc ttctaaagac ggtttcatca tccgaagaga agcagttgaa catggtattc
    11521 cattgtttac ttctttagac acagcagatg cgatcgttcg agtgatggaa tcccgtgctt
    11581 tttcaactaa ccctatttaa cggcgagcac atgttaagac aaaaagacga aaaaccgttg
    11641 caggagggga cagatgaaac aagaaatgat ggaaatcgtt cagcaaaaac agctggcacc
    11701 aaagattttt gaaatgacat tgaaaggtga tctagtaaaa gaaatgcagc aaccaggaca
    11761 gttcttgcat attctcgtgc cgcgtgcaga cttgctatta cgccgaccga ttagcttgaa
    11821 tcatatcgac cacgaaacaa atacttgccg tatcatttat cgggtggaag gtgaggggac
    11881 tagagtcttc tctactcttt ctgccggaga ccatttagat gtcatgggtc ctttaggcaa
    11941 tggttttgat ttgtccaatc ttcatacagg agacgaagta tttataatcg gtggaggaat
    12001 cggtgtccct cctctatatg agttatcaaa acagttagtc gaaaaaggaa ttaagccaac
    12061 gcatttttta ggttttgcca cacatgaagt cgtttattat gaaaaagaat ttaatcgtct
    12121 aggagaaaca agaatcgcta ctgacgatgg aacatacggt atgcacggaa atgtcggtaa
    12181 tttattgtta ggaacgaaga cggaacctac agctgttttt gcatgtggtt caaacgggtt
    12241 attgaagaca gtggaacagc tgttttcttc gcatcaaaat gtccagttat cattagaatc
    12301 ccgcatggca tgtgggatag gggcttgcta tgcttgcgtt tgtcatacgc cggaagatga
    12361 aacaggaaca aaaagtgtga aagtttgtga cgaaggaccg atttttaaaa taggagaggt
    12421 aattatatga atgagaatcc tttagcagtc aagctccccg gtctagaatt aaaaaatcct
    12481 atcatgccgg caagcggctg tttcggtttt ggcaaagaat acggaaaata ctatgatttg
    12541 aatcagcttg gatcgatcat ggtcaaggca acgactccaa atgcccgctt tggcaatccc
    12601 acaccaagag tggcggaaac accatctggc atgttgaatg caattggttt gcaaaatcct
    12661 ggtctagacg ttgttatgca taaacttctt ccagaattag aggagtacaa tgagttaccc
    12721 atcatcgcaa atgttgctgg tgcttgtgaa gaagactatg tagaagtttg tagcaaaatt
    12781 ggcgaagcgc caaatgtgaa agcaatcgag ctgaatattt cttgcccaaa tgtcaaacat
    12841 ggcggaattg cttttggaac agatcctgat gttgcttttc aattaacaca agcagtaaag
    12901 aaagtttctt ctgttcctgt ctatgtcaaa ttgtccccaa atgtaacaga catcgttcca
    12961 atcgctcagg caatcgaagc tggaggggca gatgggttta cgatgatcaa tactcttctt
    13021 ggtatgcgga ttgaccttaa gacaagaaaa ccgattttag ccaatcaaac aggtggactc
    13081 tccggaccag caatcaagcc tgtcgctatt cgtttgattc accaagtagc aagtatatcc
    13141 agccttccaa tcattgggat gggcggcgtg cagacagtgg atgatgttct tgagatgttt
    13201 atggctggag caagtgctgt agctataggg accgcgaatt tcaccgatcc gtatatctgt
    13261 cctaaactaa ttaaagagct ccctgtgcga atgagcgaac taggcatcga atcgctggaa
    13321 agattaataa aagaagtgag agaggaacgt gaacgatgaa tcaactagac caacgaccaa
    13381 ttattgcatt agatttttca actaggcaag aggtagaaga ctttcttcga tttttcccga
    13441 aagaagaaaa attgtttgtg aaaatcggaa tggaattgtt ctaccaagaa ggacctgaaa
    13501 tcgtacgtta tctaaaagag gctggtcata atgtattttt agatctgaaa cttcacgata
    13561 ttccgaatac ggtcgaaaaa gcaatgcggg gattggcaaa gcttggtgtt gatctcacct
    13621 gtgttcatgc cgctggcggt attcgtatga tggaggcggc aatgcgagga ttagaagagg
    13681 ggactccaga aggtggaaaa cgtccgctac ttctagctat cactcagctt acttctacaa
    13741 gtgaagaaga aatgcatgcc gatcaactaa tcgaagtttc tttagaaaaa agtgtgatcc
    13801 attatgctag ctgtgccaaa aaagcaggat tagatggtgt ggtttcttct gcttgggaag
    13861 tagaagcgat caaagaaatg gcaggagacg aatttgtttg cttaactccg ggtataagac
    13921 cagaaggaac agtcgcagga gatcagacaa gagtagtcac accttctcag gcaaaagaga
    13981 tcggctcaac ttttatcgtt ataggtcgac caatcactca gtcaactaat ccttataaag
    14041 catatcaaac gattcaaaca gaatggagct aacctaaaat gaatgttgaa caaagtatcg
    14101 caaaagactt attagaaatt gaagcagtat ttttaaatcc aagtaatcct tttacatggg
    14161 caagtggaat caaaagccca atttactgtg ataaccgaat cacgatgagc tatccaaaag
    14221 tccgaaaaga gatcgcaaaa ggattagcaa gcaaaatcaa agaagcttac cctgaagtac
    14281 aagtgattgc tggaactgca acagcaggaa tccctcatgc cgcatgggta gcggaaatac
    14341 tggatttgcc aatggtctat attcgaagta aggcgaaaga tcatgggaaa ggtaaccaaa
    14401 tcgaaggacg tatatttgaa ggtcagaaga tggttgtgat cgaggatttg atttctacag
    14461 gcggaagtgt cttggaagca gctgaagcag caaaaagaga aggtgcggat atccttggtg
    14521 ttgcggctat ctttacgtat gagttgccaa aaggaaaagc aaatttcgaa aaagccgaaa
    14581 tccctcttat gacgttgacc aattattctg tattgattga agcagcttta gaagatcgtt
    14641 atatcgatga gcaagaatta actctgctaa aagaatggaa aaaagatcct gaaaattggc
    14701 agatttaagc gcgcttgatg aagatagaga aatccgattt gtgtctccag aatcatcaat
    14761 tggaatagca aaaatccaaa aactgattgc gagaagcatc ttttcatcag gtatgaaaag
    14821 aaacttctat cgtgatcagt ttttgatttt tttaaaattc atttttctaa gactggttag
    14881 cagaaagaag agaataatct cttc
//
LOCUS       DAGCWU010000058        14638 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.058, whole genome shotgun sequence.
ACCESSION   DAGCWU010000058 DAGCWU010000000
VERSION     DAGCWU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.058"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..642)
                     /locus_tag="MBZ71_001409"
     CDS             complement(<1..642)
                     /locus_tag="MBZ71_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4429370.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINT"
     gene            complement(890..1726)
                     /locus_tag="MBZ71_001410"
     CDS             complement(890..1726)
                     /locus_tag="MBZ71_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBT4429371.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1737..2666)
                     /locus_tag="MBZ71_001411"
     CDS             complement(1737..2666)
                     /locus_tag="MBZ71_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBT4429372.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2858..3211
                     /locus_tag="MBZ71_001412"
     CDS             2858..3211
                     /locus_tag="MBZ71_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBT4429373.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3285..3941)
                     /locus_tag="MBZ71_001413"
     CDS             complement(3285..3941)
                     /locus_tag="MBZ71_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBT4429374.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4167..6101
                     /locus_tag="MBZ71_001414"
     CDS             4167..6101
                     /locus_tag="MBZ71_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4429375.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6187..7410)
                     /locus_tag="MBZ71_001415"
     CDS             complement(6187..7410)
                     /locus_tag="MBZ71_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBT4429376.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7404..9302)
                     /locus_tag="MBZ71_001416"
     CDS             complement(7404..9302)
                     /locus_tag="MBZ71_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBT4429377.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9296..10414)
                     /locus_tag="MBZ71_001417"
     CDS             complement(9296..10414)
                     /locus_tag="MBZ71_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBT4429378.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10497..11330)
                     /locus_tag="MBZ71_001418"
     CDS             complement(10497..11330)
                     /locus_tag="MBZ71_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4429379.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11323..12240)
                     /locus_tag="MBZ71_001419"
     CDS             complement(11323..12240)
                     /locus_tag="MBZ71_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4429380.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12467..13591)
                     /locus_tag="MBZ71_001420"
     CDS             complement(12467..13591)
                     /locus_tag="MBZ71_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4429381.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13774..>14638
                     /locus_tag="MBZ71_001421"
     CDS             13774..>14638
                     /locus_tag="MBZ71_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4429382.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKK"
BASE COUNT         4625 a         2888 c         2355 g         4770 t
ORIGIN      
        1 agtattaatg atataagctt ttaaatcttt acataaatac tcatattcga gtgatttttt
       61 atatttttta attgtgccgt ttacgagaat ctcttcatct ttcaatacta ggcctatttt
      121 atttttataa cctggcatgc tcattagata aaggatttca tcgtaatgga tatagtgatc
      181 gctattacca taacggacag ttgtccacct ttttttcgta gcatgttgaa caggatagtt
      241 ttctttttct ttcaataagt aactttggat gcttttttta tttttttcat aagtgaattc
      301 ttttatcaaa taaccactag gcgtaatctt tcgattaata acagttattg ctttattcgg
      361 atgagacgtt agataaacaa ttttacactt ttcattcttt tctctaagca tttcgcctag
      421 ttcgataccg ctataatagg cgttcaaatc gatgtctagg aaaaaaatat ctgaggaata
      481 gatttcatta tttttaatga attcaacaaa ttgtataacg ttcaatccct gaacgatctc
      541 aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc
      601 aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg
      661 atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg
      721 taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg
      781 ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg
      841 acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac
      901 gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg
      961 ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc
     1021 attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg
     1081 aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt
     1141 tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt
     1201 aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct
     1261 tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat
     1321 aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt
     1381 tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg
     1441 gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat
     1501 aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt
     1561 ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa
     1621 tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt
     1681 acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa
     1741 ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag
     1801 cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag
     1861 accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac
     1921 gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct
     1981 aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca
     2041 gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa
     2101 tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt
     2161 tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct
     2221 ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa
     2281 aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg
     2341 gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga
     2401 actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa
     2461 ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc
     2521 catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct
     2581 aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa
     2641 accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct
     2701 ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc
     2761 ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct
     2821 catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac
     2881 tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa
     2941 ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat
     3001 tccggcactt catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct
     3061 gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt
     3121 catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct
     3181 ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga
     3241 acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt
     3301 tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc
     3361 aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc
     3421 aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg
     3481 ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc
     3541 attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa
     3601 gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc
     3661 aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca
     3721 aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa
     3781 taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt
     3841 gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc
     3901 agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt
     3961 gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca
     4021 ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc
     4081 gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga
     4141 attcacaaag tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc
     4201 attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg
     4261 tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa
     4321 ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg
     4381 ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg
     4441 aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta
     4501 cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt
     4561 tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta
     4621 aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac
     4681 gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta gacgaaccaa
     4741 cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg
     4801 gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct
     4861 atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt
     4921 tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc
     4981 ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc
     5041 aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa
     5101 aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg
     5161 aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc
     5221 gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga
     5281 aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca
     5341 ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag
     5401 aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt
     5461 tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag
     5521 cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac
     5581 caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg
     5641 acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag
     5701 tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag
     5761 agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg
     5821 ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc
     5881 gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga
     5941 tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca
     6001 atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat
     6061 gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa
     6121 accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta
     6181 cctttttcac caataagaag accaattact agtcattctc actaaagctt ctaaataata
     6241 gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga
     6301 ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat
     6361 aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc
     6421 tattaattct aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct
     6481 gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat
     6541 aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata
     6601 acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact
     6661 ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt
     6721 atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg
     6781 agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag
     6841 attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca
     6901 agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat
     6961 tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga
     7021 atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa
     7081 atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa
     7141 caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata
     7201 acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat
     7261 taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa
     7321 ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt
     7381 atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt
     7441 tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct
     7501 aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt
     7561 ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg
     7621 ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata
     7681 cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat gattattcct
     7741 tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct
     7801 aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt
     7861 tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct
     7921 cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg
     7981 gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct
     8041 ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa
     8101 tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat
     8161 gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca
     8221 cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac
     8281 aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat
     8341 gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt
     8401 tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc
     8461 aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg
     8521 cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa
     8581 aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta
     8641 taaatcaaac cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa
     8701 ctgtctcgat accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc
     8761 agtgctgatt ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta
     8821 aaaaattgcc aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata
     8881 atctggcttt tttctcctgt atctaatttt ttccataata attgttcatg atgatataac
     8941 gctaataaca ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa
     9001 taatcgggat gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat
     9061 cgttcatcta attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc
     9121 attcctgcaa ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca
     9181 cttgctcgat aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc
     9241 tgtctggtcc ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc
     9301 aaatagctaa ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat
     9361 agatcagtgg cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga
     9421 tcccatcttc atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga
     9481 tagcagcttc atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa
     9541 gaccgcatgc agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat
     9601 caatgagagg aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt
     9661 ctgctatagc tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata
     9721 aagcccatgc atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta
     9781 accgaatata tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt
     9841 tcctcactag atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg
     9901 gatcaccagt ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa
     9961 tcatccaacc aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc
    10021 gtccagcaag taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact
    10081 gttgataatg tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg
    10141 aaaaatggta cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact
    10201 tttctcgttt tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc
    10261 accagtcgat tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg
    10321 gaattttatt ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa
    10381 tttttcttga ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt
    10441 tatgaatgtg gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac
    10501 ttatttgcag ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag
    10561 accattggga tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca
    10621 cctgtactag aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct
    10681 gaaagcatga ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca
    10741 ctagtaataa tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca
    10801 ttggcaccat cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt
    10861 cgcatcataa acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta
    10921 ttcgataatc ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc
    10981 accatcctag atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt
    11041 gctaatgcat aacccgccat tgtagaactg gctgtagcta aaactgtact gattacagca
    11101 ataacaatcg aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag
    11161 ttttttaata gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta
    11221 ctttgcgttg aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac
    11281 acaataatga cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc
    11341 tccgaattta aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat
    11401 agcagaggct tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac
    11461 gaccaaagct gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa
    11521 attacccaag ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat
    11581 ggggatcgtt aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc
    11641 gtatatctct ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac
    11701 actaagccaa atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca
    11761 ctgtacaccg ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa
    11821 aaaagtcatg aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac
    11881 agttctaaaa aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat
    11941 agctaatagc atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga
    12001 tttaataaac aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa
    12061 tgagtagcca attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt
    12121 cacaataaag aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt
    12181 atctgttgct tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat
    12241 cgttaagtcc tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa
    12301 aattttctgc ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg
    12361 cagcggtagt caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg
    12421 tattttcatt gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact
    12481 ttttctcagc tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca
    12541 gtgcgttggc taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt
    12601 gtggattttt ggcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc
    12661 ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag
    12721 gaaaattcaa agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt
    12781 gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt
    12841 ctttgattaa ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt
    12901 catcaagcaa agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag
    12961 attctggcaa ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga
    13021 tatattcttc ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg
    13081 gttgataata acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac
    13141 tcgtttgttg acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat
    13201 aaaatccata ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg
    13261 ctgaagtgtc tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg
    13321 tgatcatttg tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg
    13381 ctccagtttt gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt
    13441 ttgcgacata tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg
    13501 ttacttcttc gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca
    13561 aagctatcac caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt
    13621 atataaaacg ctttcactta tgtgtattaa cggaattagt aacacaaata tttatataaa
    13681 aaaatatttt acttgtcgtt ttggcttttc atttttgtta taatagtcat taattataaa
    13741 tagaaagctt tttatataaa aagagaaagg aaagtggttc ttttgttaac aaacaaccaa
    13801 tcctccaatc ttgtcaaaaa aacactaacc tattccatag atacccatct tcatttatct
    13861 gctgctccct attttcgact actaataaat gaaagcgcta atttatatgc tgaatttgca
    13921 cataaatcta tagaactaga taatccttcc cttttattca ttgataaaca gcaagaagtc
    13981 caaatctttt ctactcaaaa ggaaacacaa acttttatca ttatccagtt ttattttgat
    14041 gagcaattat tagcacatat tacgggacaa tctttttcta tagatcaaga aataacatct
    14101 atcattactt ttctgaaaaa tgaacagttc aaaattgatt atctaaaaca tagtaagcca
    14161 gctatttcct atgatcagga aatcaattct tttttcatac tatgccaaca gatgagcatt
    14221 catttgcttt ccttattagt tacttattgt ctgcaaaaaa ttcaattgtc ttccttacca
    14281 aggcattatc aacaggaact tgctttattg acaatacctt caaatgagaa agtcattcct
    14341 aaaaaaaagc atattcttta ttcagatgaa ttagttgatc caatcatacg atatttagaa
    14401 gaacatcttt cagaaaattt tttgcttgaa caattagccc aaattttttt tatcagtcca
    14461 gccagcttaa aaaaacattt tagaaaagtg aatggaaaaa gtgttattag ctattttaaa
    14521 gaactaaaaa tggaaaaagc aaaacgtctc gtgctagaaa atgaattaac ctatacagaa
    14581 attgcagata tattaggatt tcattcgatc catcattttt cttcagcttt taaaaaat
//
LOCUS       DAGCWU010000059        14474 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.059, whole genome shotgun sequence.
ACCESSION   DAGCWU010000059 DAGCWU010000000
VERSION     DAGCWU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.059"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(189..1637)
                     /gene="cls"
                     /locus_tag="MBZ71_001422"
     CDS             complement(189..1637)
                     /gene="cls"
                     /locus_tag="MBZ71_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBT4429383.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1789..2682)
                     /locus_tag="MBZ71_001423"
     CDS             complement(1789..2682)
                     /locus_tag="MBZ71_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4429384.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2666..3352)
                     /gene="ftsE"
                     /locus_tag="MBZ71_001424"
     CDS             complement(2666..3352)
                     /gene="ftsE"
                     /locus_tag="MBZ71_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBT4429385.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3457..4558)
                     /gene="prfB"
                     /locus_tag="MBZ71_001425"
     CDS             complement(join(3457..4485,4487..4558))
                     /gene="prfB"
                     /locus_tag="MBZ71_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBT4429386.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4684..7218)
                     /gene="secA"
                     /locus_tag="MBZ71_001426"
     CDS             complement(4684..7218)
                     /gene="secA"
                     /locus_tag="MBZ71_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBT4429387.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            7544..8845
                     /locus_tag="MBZ71_001427"
     CDS             7544..8845
                     /locus_tag="MBZ71_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4429388.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(8941..9492)
                     /gene="raiA"
                     /locus_tag="MBZ71_001428"
     CDS             complement(8941..9492)
                     /gene="raiA"
                     /locus_tag="MBZ71_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBT4429389.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(9620..10339)
                     /locus_tag="MBZ71_001429"
     CDS             complement(9620..10339)
                     /locus_tag="MBZ71_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBT4429390.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(10336..11649)
                     /locus_tag="MBZ71_001430"
     CDS             complement(10336..11649)
                     /locus_tag="MBZ71_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBT4429391.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(11730..12050)
                     /locus_tag="MBZ71_001431"
     CDS             complement(11730..12050)
                     /locus_tag="MBZ71_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4429392.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(12158..12445)
                     /locus_tag="MBZ71_001432"
     CDS             complement(12158..12445)
                     /locus_tag="MBZ71_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429393.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            12572..13216
                     /locus_tag="MBZ71_001433"
     CDS             12572..13216
                     /locus_tag="MBZ71_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBT4429394.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(13275..13982)
                     /locus_tag="MBZ71_001434"
     CDS             complement(13275..13982)
                     /locus_tag="MBZ71_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4429395.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(14202..14372)
                     /locus_tag="MBZ71_001435"
     CDS             complement(14202..14372)
                     /locus_tag="MBZ71_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429396.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         4084 a         3116 c         2521 g         4753 t
ORIGIN      
        1 aaatgtgtct cttttaatag catatattag cttatttctt gctttcccca aaaaacaaga
       61 gataccccaa agttaatttc gctcatgcga gacgctttct ccatttctga cgtactctca
      121 gaaatggccc cgttagaaaa ggacacgaga tctccatctg ttgatggttc tcatgtcctt
      181 tttatgtttt aaaggagcgg cgaaatcaat cttgctaatc cttctttcat cttgatgatt
      241 attcctcggt tttcatacat ttcttttgtg agatgcgtag aaattttcga gtcttcaaaa
      301 aaagcttttc ttacacgtga agccattcgt tcatcataaa ccagtgtatt gacctcaaag
      361 tccaagcgaa atgaacgaac atcgatattt gctgagccaa ctgaaacaat gccaccatcg
      421 ataatcatcg tctttgcatg aataaatcca ttctcatatg tttcgatcct tgctccatac
      481 tcgattagct ctgcagcgaa agaatacgtt gcccagtaaa cgagaagatg atctggtttg
      541 ttaggtattt ggatatgaac ttttacgcct gaaagaagag ccaacttcag tgcttcatga
      601 atcgatccat ccggtatgta ataaggcgtt tggatcaata tctcccgttt ggctaacgaa
      661 atcattttca aataagtcat tttgatttgt tcatgttcgg tatctggtcc gcttgtcacg
      721 atttgaacag ctatatcacc agtagagtga atggacggaa aaaatttggg agaatagcct
      781 acttcttttg tatgctgaga attccagtcc atcaaaaaac ggttttgcaa ggtatacacc
      841 gcttctccgt aaatacgcaa atgattgtct cgccaataac caaatttctt taccaatcct
      901 agatattcat ttccaacatt gaacccacct gtatatccga ttttcccatc aataactacg
      961 attttacgat gattgcgata attgatccga ggattaatat acggaacaaa tagaggaaag
     1021 aaaaatgcga cttctccgcc tgcttttttc aaattctgaa aaaagctgga agaaacttgt
     1081 gtggaacccc atgcatcaag aaggactctg acttttaccc ctcgtcttgc cgcctctgtc
     1141 aatgcatctc gaacttcttc acctaatgca tcactgcgat aaatataata ctggaagtgg
     1201 atatggtctt ctgcctgctt gatatcttca atcagcgcat cgaatttttc acgaccatct
     1261 gtaaataaag tgatttcatt tttagtcgta tagagcgtac tttcaaatac cgtgagcata
     1321 tagatcaatt gtttcacatc tacttgtcca gtaggcggat gaggataaag ccctcgcatc
     1381 aatgcctgtt tgtcttcttc aatttccagg ttcttaccga ttttcccttg gatcttcaaa
     1441 tcaaagattt tctctttgga aatccctctt cctaaaaaaa tatataaaat aaatcctaca
     1501 actggaatga acatcaaaac gagcacccat gcccatgttt gagcagtttc ctttctttct
     1561 cgaaaaacga tgataaagga aagcaaaata ttcataatca aaaggatcgt aaaaaaattg
     1621 tcaacgataa ttttcacggc ttcacctctt cttctaccaa cttaagaata cccaagttgt
     1681 tttttaaagt aaagggaaaa aagccgcttc cttctattct cctagccaat aaaaaaagaa
     1741 agctggaacg aaactgtcca actttctcat tttatttata ttgtggtatt agaatttcaa
     1801 gaaacgtctc atagaaatga tcgagccaag tgaaccaatg atcacaccaa tgccgaccat
     1861 taaaagattg attttccaaa cgatattttg tggatgaatc aatgaataat gggaacgtaa
     1921 taatgatgga ttgatcaatt ggaaaaacca tgcataacca aatgtgatga tcaaaatcgg
     1981 aagaatcgca ccgatcagac cgatccagcc accttcaagg aaaaacggcc agcgaatata
     2041 tccatttttt gcaccgacta aacgcatgat ttgaatttca cgctgtctag acaaaatcgt
     2101 gatccgaatc gtgttcgaaa tgagaaacat cgctacgaac agcagcaatg ccgctgcaac
     2161 taatccccat gtccgaactg caccagcgat ttggaaaatc cgatctgagg aagctccacc
     2221 gtaatcagaa cgagaaacat tcttcaaatc tgctgcttct tctgctactt gttttgtata
     2281 agaaggatca gtagcactta ctacatacac atcatataac gggttattgt caccttcaaa
     2341 caatttccag gaatcaccca tttcgttttg gatttttttc aattcctgtt ctttgcttga
     2401 aaattcaact tttttaacat gatctaagtc attcaattct ttttctagct ttttcatctc
     2461 ttcgtctttt gtaccgatat ctacgaatac agagacatca acgtttcctt ctatatcttg
     2521 agctaattta gttgcgttca tgataactgc catgaataca ccgactaaag tcaatgtgat
     2581 cgttaccgca ctgactgaag caactgtcat ccagccgtta cgtttcaaac tcttgatact
     2641 ttctaataaa tggcggaaaa aggttctaat catcgtatcc atattcccct tccgcttgat
     2701 ctcgaataat gcggccgttt tcgatagcaa ttactcggtg ccgaatcgta tttacgatcg
     2761 tactgttatg tgtagccatc acgacagtag ttccttgggc attgatccga tctaataatt
     2821 tcatgatttc ccatgagttt tctggatcca gatttcctgt cggttcatcc gcgataagta
     2881 ctttaggcgt attcactatt gcccgagcaa tagagacacg ttgctgttcc ccaccggata
     2941 attcgctagg aaagactcga actttgtgtt tcaaaccaac aaggtccagc acttccatta
     3001 ctcgtttttt gatatcccga ggttttctgc cgataacttg catagcgtac gcaacatttt
     3061 catatactgt ttttcttggt aataatttat agtcttggaa caccacgccg atttctctgc
     3121 gtagataagg aacttcgcgg tttttgattg ctattagatc atgacctgcg acgttcaaag
     3181 ttccttttga agctttttct tctcgataca tcaatttgat gaatgtagat ttaccggctc
     3241 cagacggacc aaccacatag acaaactctc cttgttcgat gacaattgag agattgcgga
     3301 tagcggtcgt accatttgag tacttcttca ttacatcctt catttcaatc atggcgttct
     3361 ctccatatct ttttcagtct ttagctatta tagcatggca atgtttcaac atgcttgcaa
     3421 aatcttacaa ttttatttca atttaacaat gggttcttat tgtagatgca tttttaaata
     3481 gccgtcaata aataaatcaa ggtcaccgtc cataactgct tggacatttc ctgtctctac
     3541 gtttgtccga tggtctttta ccatagaata tggatggaaa acataagaac ggatctgtga
     3601 accccagccg atttcttttt gttctcctcg aagtgcagct gcttcttgtt ctttcttttc
     3661 cacttctaat tgataaagct tagcttttaa catccccatc gcttgctcac ggtttttcaa
     3721 ttgtgaacgt tgtgcctgac tagctactac tgttcctgta ggcaagtgag tgatccgtac
     3781 agcagattct gttttattga tgtgctgacc acctgcacca cttgctcggt acgtatcgat
     3841 tttcaaatca tctggattga tttctatatc gatcgtttca tcaagctctg gcatcacatc
     3901 taccgaacaa aaagaagtat gacgtcgttt cgctgagtca aaaggcgaga tacgtacaag
     3961 gcgatgaaca cctttttctg atttcagata accaaatgcg ttgtgtcctt tgatcagtag
     4021 ggtcacactc ttgattcctg cttcatcacc tgcttgataa tctagtgtct ctacttgata
     4081 cccatgctgc tctgcccagc gtgtatacat acgtaaaagc atactgcccc aatcttggga
     4141 ctccgttccg ccagcccctg gatgcaattc aacgatggcg ttgtttcgat cataaggacc
     4201 atctaacaac atagataatt catatgtctt cattttttca tctagcgcat tgatacgtgt
     4261 ttctgcttct tcttgcatgg ctgcatcagg ttcttcctgc aacatctcca ccatcaattc
     4321 gatttcttcc agttcttcag caagagaatg gaattgatca taagtttctt tatttgcatt
     4381 gttttcactg atcacttttt gagcagcttc gctatcatcc caaaaaccag gttcagccat
     4441 ttttgcttct gcttccgcaa tgtcttcttc cagctggtct aagtcaaaga gacctcctga
     4501 agcttgtgac cttttcctgc atttctgtca ggagatttcg aatttcggat atttccatgt
     4561 tttttccttc tttcactata cttttttcat tatatctagt ctaacagaat cagcaaataa
     4621 cagaaagaag gtgagacgaa ttcttaaaaa aagaatttgt ctcaccaatc ctgctgtcag
     4681 aaattagttt gcattttttc catgacagtt cttatatttt ttcccgcttc cacaaggaca
     4741 aggatcattt cttccgacct tgttcacatg gattggctgt tttttcgcgc tagtattact
     4801 attttctttg gcatctccgt cttctgagtg agaagctgtt ccttgagaaa cctgttcacg
     4861 ctgtacgttt tgacggatct cagctttcat aaagattcga gtgacttcat actcgatggc
     4921 gcctaccata tcttcaaaca tcttatagcc ttctgtttga tattcgacta atggattgtt
     4981 ctgtccataa gcacgtaagc cgattgactg acgtaattga tccattgcat caatatggtc
     5041 tgtccattta gagtccacaa cacgcaagat cacgactttt tcaaattcca acagttgctc
     5101 tggaccattt agttgagccg ctttggtatt aaatacttct tgtgctcgtt cgtttagata
     5161 ctctttgatc tcctgagctg tttttccttc gatatctgca agagtaatac tatcttcatg
     5221 gacaagagta ctgccggcaa aatcgatgat actttccaaa ttccaatttt ctttctctaa
     5281 ttgtgtatgc gcatctacta cacgactgat tgtccgtttg accatgttca ttagtggctt
     5341 ggataagctt ttttcttcca tgattacttc ttgtcgctgt ttatagatta cttcacgttg
     5401 ttcacgcatc acatcatcgt attgtaagac gtttttacgt gtgtcatagt tatttccttc
     5461 tacacgtttt tgtgcagatt ctacttgttt cgtcagcatc ttgctttgga taacagcatc
     5521 ttcctcaccg atcttcatcc gatctaagaa agatttgatt cgttctgaac cgaatcgttt
     5581 catcaaatca tcttccaatg acaagtagaa ctgtgacata ccagggtctc cttgacgtcc
     5641 tgcacgtcct cgaagctggt tatcgatacg acgagattca tgtcgttctg tcccgataac
     5701 tgctagacca ccaagttctc ttaccccaag tcctaacttg atatctgttc cccgaccagc
     5761 catgttggta gcaatcgtca cagcaccttt ttgaccagca ttcatgatga tctctgcttc
     5821 tttaaagtga tttttcgcat tcagcacttc atgaggaata cgttcttgat tcagcatatc
     5881 tgataataac tcagatgttt caaccgcaac agttccgact aacacaggtt gacctttgcg
     5941 gtagcgatct ttgatatctt gcacgacagc gtggaacttg ctttgtaatg ttgggtaaag
     6001 caaatctgga cgatcatcac ggataaccgg acgatttgtc ggaatctggt atacttggat
     6061 gttataaatt tctctgaatt cttcttcttc tgttttagct gtaccagtca ttcccgcaag
     6121 tttcttgtac atacggaaat agttctggaa agtgatcgtc gccatggttt tcgtttcgtc
     6181 ttcgatctct acgccttctt tagcttcgat tgcttggtga agtccatctg aataacggcg
     6241 tccatccatg atccgaccag tgaactggtc aacaatcagt acttttccgt cttggacaac
     6301 ataatcgata tctaaaagca tgatatagtt ggctctcaat gcttgatcca agtgatgggt
     6361 caatgcagta ttttcaatgt cgtataagtt atccaaaccg aatgtttctt ctgctttttc
     6421 gatccctgct tcggttaaac tgattgtttt tgattgtaca tcgattttat aatcttcttc
     6481 ttctttcaaa cgtttcacga agttgtctgc tcttgtatat aaagctgtgg atttttctgc
     6541 ttgccctgaa atgatcagcg gcgttctcgc ttcatcaatc aagattgagt caacttcatc
     6601 gacaatggca taattcagcg gacgttgtac catttgatgg cggtaaacga ccatattgtc
     6661 tctcaagtag tcaaatccta attcattatt cgtactataa gtgatatcac aattataagc
     6721 ctcgcgtttt tcatccgaag attttgagtt gatattcaac cccactgtta atcctaagaa
     6781 attataaagt tcgcccattt ccgtcgagtc acgggttgct aaatattcat tgacggtaac
     6841 tacgtgtacc ccttcgccag tcagagcatt tagatatact ggcatcgttg cggtcaaggt
     6901 tttaccttca ccagtacgca tttcagggat atttccatcg tgaagcacga ttccacccat
     6961 caattgtacg tggtgtggat ataaccccaa tacacgtttt gctgcttctc ggacgactgc
     7021 aaaagcttcc ggaagtaatt gatctaatgt ttctcctttt tggtatctgc ccttgaattc
     7081 agcagttttt gcctgtaatt gttcatcagt taatgctgcc atttggtcag cgtatgcttc
     7141 aacttttttt gcaatgctat ctaaacgttt tagttctttc ttatcgtttt cgataagtga
     7201 acgaatcaaa ttggccatgc gttgtgtaat ctccttttcc ttctgtgtat tatccagact
     7261 atgtagactt tacttttcat tttatcatta gttatagata attgaaatat cttaagcgta
     7321 ttaaagtaag aacttcataa tttattcata tttcatctaa aagaggagga aactcaaaaa
     7381 aacttgcggc agctgtcagt agctgctgca agttttcggg ctctataatt tctaggactg
     7441 atagaaaatc attctatcgg tcccttttga gcatgaaaaa acaaaacata cagaagctcc
     7501 gttagaattg aatcaccaca acacaatcaa aggagcgttc tgtatgcctg atcttcatca
     7561 tatcaaacta ctacttggaa tcaaagataa aaacattttc atcacaaatg tggaatcgaa
     7621 gagcattaag aatatcaaga gtcttgttgt ttctgcgacg ttaagtaaag aaattcgtcg
     7681 ctgtcctttg tgtaaacaga tgaatcatga aggcatgatc gttaagaacg gaaagaaaaa
     7741 atcgctcatt caacttaata aatgtgcgaa ccaactgact tatcttgctt tagcgaaaca
     7801 acgctaccac tgtcgtggct gccacaccta ttttacggcg aatacctaca ttgtcgatag
     7861 aaactgcttt atcgctaaac aagtgcgcta taaaatttta gaagagctaa ctgaaaaaca
     7921 agcaatgacg acaattgcta agcattgcgg cgtttcttgg tcgaccgttt ctcggacact
     7981 cgcttctctg atccccatga caaaagtaaa aagaaattgg ttaccgcgtt gtttattaat
     8041 ggatgaattt cgttcactga aaaatcaagt cggtccctac tccttttctt gtatggatgg
     8101 cgatactgga aaattactgg atattcttcc ttcacggaag aaaaaggctc tcgtttctta
     8161 ttttatgcaa tttgaacgac gcgcacgatt gaaggtaaag gtcatcgtga cggacatgaa
     8221 tgtttcttat gcctctctga taaaggaatg ttttccaaag gcaaaactcg ttgtggatcg
     8281 ttttcacatt gtcaaacatc tcattcgtaa atttgaggat attcgcgtac gaatcatgaa
     8341 aagctttgat cgaaacgatc caacacaagc caaatattat cgacaattaa aggcgttgtc
     8401 tcggcttctg atcaaacgac aggacaagct tgtttatgac aaatggacca aatggcgcaa
     8461 ttttggttgg gcgtatctca ccgaaagtga agtggtggat cgtctgcttt cgatctctga
     8521 cgaattacga atcgcctatt tttactatca agaaattctt caagcgtttc acgacaaaga
     8581 ggcggacact ttctttaaac ttgtaagaac gatgcccaat tccgttccaa aagaactgca
     8641 ttctataaaa aaagctttta taaactatga atccggcatt cgtttagccc tcgaattgcc
     8701 ttattcaaat gccaaaatag aaaatctgca tacccacatc aaagcattga aacgagtcgc
     8761 ttatggcttt cgaagctttc ggaaaatgaa gaccagaatt ttcttactaa ataatctgat
     8821 tacctatgaa tcaaaaaaca tctaaagtag aaaaggaatt ctaccttaga tgttcttatc
     8881 tcactctatc agtcctattt gacaaagagc cagttttcgt tgttatttaa gtaaggctga
     8941 tcagtctgtt tcaatcagtc cgtattttcc atctttacgg cggtaaacga tacttgtacc
     9001 atttgtttct gcatcttcaa aaataaagaa gttatgtccc agcatgttca tttgtaagac
     9061 tgcttcttca ctatccattg gtttcaatga aaggcgtttc gtacgtacga tatctaattc
     9121 agaatcattt tcgttttcgt cctcttcatt gaataaagca gcagcatctg cagtattcat
     9181 gcccgtttca cgggatttac gattaatttt tgttttgaat ttgcggattt ggcgttccaa
     9241 tttatcaaca accaaatcga tacttgcgta taaatctgga gaagtttctt cggcacgtaa
     9301 tacaagataa ggtaatggga tcgttacttc cacttttgct gttttttctg tataaacttt
     9361 taagttcaca tgcgccgttg catcgggtgc gtcactgaaa tagcgttcta gtttgcctac
     9421 ttttttctca acgtagtcgc gaatcgcttc agttacttcg atattttcgc cgcgtacatt
     9481 atatctaaac ataaaaatta cccctttcgt ctaccacaat caagaaggag aaggaccact
     9541 ctttccctct tcttaattct attatatcga actctaggtt atttgcaaat taaatcgcta
     9601 tcaaatttcc acatctaatt catctcgcca aagaaatggt tcggatgatt ttcggctgtg
     9661 cgacctgcaa acaatctgct gcgtggaaaa gagtctgtcc tgttgtatag acatcatcaa
     9721 taatcaatat ttttttattt tgtatcttag aaaaggtgac tgcaagttca aaaggttgag
     9781 gcatctccaa tctttccttt cgcgtgtttt ttgcttgagc cagcagatgc ttctctcttt
     9841 tgagtagcat ctgaaaagga attcttgctg ctttcaacat ctcgcttact tgattgaatc
     9901 ctctttctgt tcgccttttt tcagataaag gtattggaca atagatgtat gtcgggtatt
     9961 ctttttttat ctgcgtccat ttatatgcaa aggtgcctgc tagtcggata tctcccataa
    10021 acttgtattg attgagccag gccttcatat tttcatcgta cacaaaaaag gcacggtgct
    10081 gaaaatcata ggtcggatat ttcttcttcc attcttgaca gtcactgcag taaacgggaa
    10141 tagactttac tttctttttc gtactctcta gattgaaatg gattggtttt tgacagccag
    10201 aacagcctct tgtttccaat aaatgaaatt gtgcgcgaca aactgaacaa gtctgaggta
    10261 atctgtattt cgaaacacca ataatctcgc tgaatgttaa attttggaag atatctttac
    10321 tgcagcaact gcacctcaca gcatcttctt cccttcttca ttcatttctt tgatttcctg
    10381 acaagctttt cgaatagctg cagttttttc ttcatagaaa aagtatactt gactgttcgt
    10441 ataaggccct ttacgatcgg ctcttcctgc gatttgtacc aaagaagatt tagtaaaaac
    10501 cggatgatcc gctcctaaaa cgataacgga tattctttca aatgtgactc cacgttcaag
    10561 gatcatcgtg gttaacagta tatcatattt tgcttctcgc attttctgga ctttttccgc
    10621 ccgctgattg tctcttgaag aaacatctgt gatctcatat ctcggaagag cctcttgaag
    10681 tgtggctttc aaacgatgca tcatcgtgat acttggacaa aataaaagga tatggttttc
    10741 tttaattaat tgatgaatga tccgcaccaa agattttatc ttcttcttgt tcttgcattt
    10801 ttgtcgccaa ttattcatga aaaccaagac gggttcagga agcaaacgcc gatgaaagcg
    10861 taaagcaagt ttgtggatgt caaacgtctg cttgatttcc tttagtaact ttttatcagg
    10921 tgtggcactt aagtagatga atcgtccgtc tggtttaatc gctgtttgca cagcatgagc
    10981 taatcctgca tccccgttat aaggaaacgc atcgatctca tcgatcacta ttaaatcaaa
    11041 ggaccgataa aaacggtata gctgatgaat cgtacagata acaaaagaag taaaggtata
    11101 agccgttgct gtattcccat ggagcaacgt gattttttct tctggaaaaa cataacgata
    11161 gcgtaaaaac aattcattac aaacatcgac tcgcggtgta caaatcgcta ctcttcctcc
    11221 agcttttaaa gtttccacta aaataggata aagcatctct gttttccctg cacctgttac
    11281 agcccaaata agatgatgtc ttttttctga atggcaaagt attctctttg agatttccgt
    11341 ttgtttttca gtaagctgtc ctgtccaaaa aaagacgact tctcgaggtt ttgcttcagg
    11401 ctgatcaaac aaatataaat cttgttgagt gtcgcatcgc cctaaatata gacaatgtgt
    11461 acaaaaataa ccgtaatctg caagctgtcc atcaatgacg gacgtcctca ttccacagcg
    11521 atgacaaaga atccagttgc ccaaacgttc cactgctgcg atcttctcat aatttggtaa
    11581 atttacctcc tcacagtcta ttgcttcaat atctctcgtc agaagccttc taccccaaaa
    11641 atgattcata ttcttcgccc taaaaaaata tacgaaaaaa caagcgatct attttttgga
    11701 tcgcttgttc gatagttata ctgttctatt tattcaccta tgagcgtgat tgcttgatct
    11761 aacacttttt cgccattcat cataccataa tcagccatat tgatgacatc taaaggaatg
    11821 ccttttggtt ccagacgttt ctcaaaatca gctttcataa agcgaacttg tgggcctaat
    11881 aacaacacat ccacgtcttt atttgcaagg ttgttatctg cgtcagaagc ggacacagcg
    11941 aaaatatccg agtcgattcc ttttgcttca gcagcttttt gcatttttgt tactagtaaa
    12001 cttgtgctca taccagccga acatacaagc atgattgttt ttttagccat acttttctct
    12061 cctttttaaa acaaactatg caaacgtttt cttcgcgtct gttactctat tatataaaaa
    12121 gaaagaaatt tgtcaacgtt ttctaaacgc ggtttattta atcaaatagc caactagatt
    12181 tcactaattt attgaacacg acatattctt tgtccaaatt gatgacaact acttcatagc
    12241 ctgcatttat ccagccatag gcaccttttt cagggtgttt ataatcattt gcccaccaat
    12301 ctgtactttc ccttgctgtt ttgggaagtc cgttactggg aaacagcagt tcgtcaaatt
    12361 gggtaaaggt caacgttact tgagagccgc cattgttctt caaataatgc gtaattgcat
    12421 cgtacttttt cattcgttcc tgcatcccca gatccacctg cctttgtctt tattatcgca
    12481 tactacagca attgcgcaac cgttacagtg ttgaaacaac ctagaacttt atttatactg
    12541 aataaagaaa atgaaaaaaa gaggtgacaa gatgatcgag cgctatcgta caataaaaga
    12601 aaatggacaa agtgagattg agatcaaaaa atcacgcttt atctgttctc ttagacgaat
    12661 agaatcagag gaccaagcaa aaagtttcat tcaagaaata aaaaaagaac attggaaagc
    12721 gaaccataac tgcagcgcct tcatcattgg agaaaaaaat gaaatccagc gaagcagcga
    12781 tgatggagaa ccaagtggaa cggcgggcgt accgatgttg gaagtcctca aaaaaaatca
    12841 actgatcaat gtagctgcag tagtcactcg atattttgga ggaacgaaac tcggcacagg
    12901 cggattaata cgagcataca ctcatgcggt ctctcaagca ttgtctgaca ttggaatcgt
    12961 agaaggtacc ttgcaacagg agatctctgt gatgatcacg tatccgcaac taggaaaatt
    13021 acagaattat cttgaactcc aacatttttt tataaaagaa atccaatata ctgatcaagt
    13081 tagcgtcatc tgtgctgtag atgaaaaaag tgctgaccac tttaaagaat ccatcattga
    13141 gctgctgaac ggacaagtcc aatttgaaga gggttctgtt tcttatcacg agcaactgat
    13201 aactgatcat atataaaaac aggtgattct gattctcttt tatttctaat aaggaatcag
    13261 ggatcacctg ttttttattt ttccaaataa aactcaaaac gatttcctac gtattgcgta
    13321 cgcacatact caaacggcgt accgtcttca aaataagaaa tctggcgcaa acgaagaact
    13381 gcatctcctc gttttacctc taaatagtca gcaattttct cagaagccaa cacagcagta
    13441 atcgtttgat tcgctggacc gatctttttc ccgccttttt cttctagcgt gcgatacaac
    13501 gattcagtaa tctctgcttt gctgtagcca tcgatcagct tttgtggaac actggcaact
    13561 tcaaaacaaa tagggacatc atctgcgaaa cgaatacgtt ccattcgcaa gatcgtgtca
    13621 tctttactca agtttagttt ttccatttca cttgagcttg gcgaagtcaa gaaataagag
    13681 attgtccgac tagacggtac tcgattttgt gattccataa tttctgtaaa acttgttgca
    13741 ccagacattt tttcttgtac tttttctcgc gcaacatacg tacccgaacc gatctttctt
    13801 tctaagattc cttcatctgc caagttttga attgcttgtc tcaacgtcat acggctgaca
    13861 ttgaatttca ctgctaattc tctttctgaa ggaagacggt ctccgacttt ccatataccc
    13921 tgttcgattt cattttttaa ttggtcatga atctgtatat atacaggtaa ttgattcgac
    13981 ataacgtaca cccgtccttt cgtttcttct attataactg gtatatacct gattcacaac
    14041 tgaaaaagat aaaggtttct atccatcaac agaggattta gacacacatc gttctcccta
    14101 ctcctattgt cagaaacaat tttttagata tgttcaacct agaaaataac aaaagaccct
    14161 aaaatcgatg aaaatgccga cttatggatc ttttgttatt attagcggtt tttagccatc
    14221 catttttttg tatgattcgt tgggcgtttt gattttacct ggaccgcttt tccgcctttt
    14281 ttcggaatat tcacttcaat attgtatttt tctgatttga ccaaatcata taacttttca
    14341 gctgcttcgt ctactttttc tttccatttc atgtggatcc ctccaattct tgtagttaac
    14401 ttaactctat cttaattcag cagaatcttc aaggaacaag gtagcaaagc aaaaaaaaga
    14461 gctctcgaac taac
//
LOCUS       DAGCWU010000060        14338 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.060, whole genome shotgun sequence.
ACCESSION   DAGCWU010000060 DAGCWU010000000
VERSION     DAGCWU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.060"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            15..161
                     /locus_tag="MBZ71_001436"
     CDS             15..161
                     /locus_tag="MBZ71_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBT4429397.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(223..2034)
                     /locus_tag="MBZ71_001437"
     CDS             complement(223..2034)
                     /locus_tag="MBZ71_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBT4429398.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2031..2396)
                     /locus_tag="MBZ71_001438"
     CDS             complement(2031..2396)
                     /locus_tag="MBZ71_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBT4429399.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2559..2954)
                     /locus_tag="MBZ71_001439"
     CDS             complement(2559..2954)
                     /locus_tag="MBZ71_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBT4429400.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2972..3934)
                     /locus_tag="MBZ71_001440"
     CDS             complement(2972..3934)
                     /locus_tag="MBZ71_001440"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBT4429401.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3934..4146)
                     /locus_tag="MBZ71_001441"
     CDS             complement(3934..4146)
                     /locus_tag="MBZ71_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBT4429402.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4167..4865)
                     /locus_tag="MBZ71_001442"
     CDS             complement(4167..4865)
                     /locus_tag="MBZ71_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBT4429403.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4885..5427)
                     /locus_tag="MBZ71_001443"
     CDS             complement(4885..5427)
                     /locus_tag="MBZ71_001443"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBT4429404.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5559..6563)
                     /locus_tag="MBZ71_001444"
     CDS             complement(5559..6563)
                     /locus_tag="MBZ71_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBT4429405.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6560..7549)
                     /locus_tag="MBZ71_001445"
     CDS             complement(6560..7549)
                     /locus_tag="MBZ71_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBT4429406.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7546..8352)
                     /locus_tag="MBZ71_001446"
     CDS             complement(7546..8352)
                     /locus_tag="MBZ71_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429407.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8518..9474
                     /locus_tag="MBZ71_001447"
     CDS             8518..9474
                     /locus_tag="MBZ71_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4429408.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9551..10069)
                     /locus_tag="MBZ71_001448"
     CDS             complement(9551..10069)
                     /locus_tag="MBZ71_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4429409.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10157..10306)
                     /gene="rpmG"
                     /locus_tag="MBZ71_001449"
     CDS             complement(10157..10306)
                     /gene="rpmG"
                     /locus_tag="MBZ71_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBT4429410.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10534..10980
                     /locus_tag="MBZ71_001450"
     CDS             10534..10980
                     /locus_tag="MBZ71_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBT4429411.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11175..13070)
                     /gene="asnB"
                     /locus_tag="MBZ71_001451"
     CDS             complement(11175..13070)
                     /gene="asnB"
                     /locus_tag="MBZ71_001451"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBT4429412.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13395..>14338)
                     /gene="bsh"
                     /locus_tag="MBZ71_001452"
     CDS             complement(13395..>14338)
                     /gene="bsh"
                     /locus_tag="MBZ71_001452"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBT4429413.1"
                     /translation="HYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKNLDKHYAMIGIA
                     AGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFIPWILGQCSTID
                     EAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHLYDNPVGVLTNN
                     PPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATF
                     TKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSCCNVDKGIYYYR
                     TYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         4466 a         3019 c         2422 g         4431 t
ORIGIN      
        1 aatttgtatc tggaatatta gtaggaagtc tggcaacagc cgctgccgtt gctggtttgg
       61 ttgcttctgt gaaaaaaaca gtgatcgatc caattgacga aaaagaagca atgatcgaag
      121 aaaaccgtaa aaaagcaatg cgtaaacgcg tttctcgtta ataattattt aaaaatacgc
      181 atgaagactt tctaaaaaac agataagtca tcatgcgtat ttttatttgt cagtcaatat
      241 aagttgtgct attttatctg cctgtctcag attccatgga tctgtataag gtctagctac
      301 tgctgcagtt agccaatcca caccttcaac aggtattcca tagcaataaa agtcagactg
      361 gatttcccct gaagcatcaa tcacgcgatt cgtacctcgc tcaacatcta tagcttctga
      421 atgataactt cctgtattat ctgaaaatga aaaagggcga acaagtttgt tttccttcaa
      481 attttttatg acaggattac ttgaacgaga aaagcttgtt tgtggcaatc gagcttcaat
      541 caaatagcga ctgctgattt ctttatcttg tagagaaata acatagcggt tgtgcttttg
      601 ctgaatatca aatggcggtt cgatcaaatg aaaaataccc gcatctatca gtgctgctaa
      661 ttctttcgtc cgaatgattg gcggaccgat cgttaaaaag ctatttagat gagtaaacca
      721 tccccatagt atttctttgt attccttagc agaaaaaacg tgccattcaa tagcctgccg
      781 gattggatca cgccagtctt ttaatgcatc aaatgttgaa gcaaatgctc ctgtgcaatt
      841 gcctttttcc gcttctttta tctgcccttc aataaatatt cggctagctt ctgaaaaggg
      901 ctcattctga taggcaagag gagtttctaa atacgaccaa tcccatctat aagggattag
      961 tgctggatgc tttccgagac actcctctga agagagggag agaaaatcat gctcaaaatt
     1021 tttctttgga atcggcaagt gatattcttc gatgattttc ttgtaataaa acaactcaac
     1081 gtcttttttc agatagtcaa aaaatgtttc gccagtcaaa aatcccgact gatgccattg
     1141 ctccaggttt tcttttgtta gtaaccgtgg ccatgccatt gctccgcctt gcttttgatt
     1201 tcttcctcga gggtaataag ggagccccct tcttgatcca ctgtatacaa tcggttcctt
     1261 gcctgatgga tgatagacca gttttccctt ttcgttctcg aagcgccctc ctcttgatac
     1321 tgtaaacaat ccgacataat caaaaaaact taagcctaga cctcttaaaa agacattttc
     1381 tttaggcggt atcttcgaaa gatctacatc tgcaggatta gaaggaagct gatacagtaa
     1441 aacatttttc tttgcgtagt ctacccacac tctttcttct tctgtctcat cattttccca
     1501 gtgaccgctt gctaaaacaa gctgatcgat agaatagctt tgctgatctg tctgtaaaag
     1561 ataaccatct tttcttttgc ttactttttt tactaaagaa taaacaaact gaatagaagc
     1621 tggaaattct tcttgcaact ttgagaaaaa ccagcgttga tagagtccgt ataaacatcg
     1681 agtagattgc tgatcttttt ccagacgatt gatttcttct ataattagtt gcttattttt
     1741 ggttaccgtc tgttcgatat aatctgtagc aggtccttta ctccattgaa acaagttggg
     1801 tccctgtgtt accaatcctc cactggacaa tgtttcatct gtaaaaagcg tcacttgttg
     1861 agaaacagaa ttcattaata actccggtgg ctgattcaac cgccaaactc gacctccagg
     1921 accattggga tcgaaaagaa aaagttcaag agaatgtctg tcctgattat tatggagaat
     1981 cctttctgct acacttaatc ctctaggtcc ggcaccaatg atccctattt tcatttgcat
     2041 gcctccattt cgataaattt tttcaataaa acagcatggt tctgatgttc attttttgca
     2101 ccataaacaa aagtaatgac tggatgttct ttcataagtt gactgagttt aaaaatagct
     2161 tgcttctttt cttcttcgaa aatcaattct tgctggtatt tctttgcaaa tgaagcaaat
     2221 cgttctggta catggtcaaa agttttgcgt gcttcattac taggtgcaat ttctttcaac
     2281 cacagatcta actgcgcttt ttcttttgaa attcctcgtg gccagaatct atccacgagt
     2341 attctatagc catcctcttt ttcagcatca aggtaaacac gttttaattt gatcatagtt
     2401 gtttccttct ttctaccttt tcgcttagct tatatatgta gataatagca caaaataaaa
     2461 aaaatgaacg gcacaactat ccattgcgcc gttcatgtga tcttggtcaa aatctaatga
     2521 ttgattcggt ttttaaagct gtcatacaat catctgtttt atactttttt acttttgatt
     2581 tttccagacc atccgtcata tccaccctta agtaaataaa tctcttgata acctgcttta
     2641 cgtaatttat ttgctgtacg gatgctcaaa cttttctttt gatcgtaaat atagacaggc
     2701 tggtcttttc gaatagatcc taatgaatct ttcaaaacag tgtaagggat gtttcttgca
     2761 cctaaaatat gcccagcatc aaacacatct ttttcgcgaa catcgattac ttgtgctttt
     2821 ctcattcctt ctcgaaattc ttcttctgtc aaagtcgttg ctgaacgttt ggccattact
     2881 cgtagataga cttcccaaag gatgattgca agcacaatca atagcaaaat gatattaatt
     2941 acccataaaa ccatgtaatg cctccgtttt tttaagcaaa tttcaaggct aatgaggcag
     3001 caccaatgac accagcttca ttaccaagct ctgctaattt gatttttgtt gattctctta
     3061 cttgcgggaa agtaaattct ttaaaatatt tctctacacg gctacgtaaa aattctcctg
     3121 ctgccgatac tcctccacct aacacgatac ttgacggatt taatgtattc cccaagtttc
     3181 cgcaagctaa acctaagtag aagcatacgc gatcaacaac catcaatgca aacggatcgt
     3241 cttcttgagc catttcaaag atatctttac ttgttacttc ttctccgttg tctagcattg
     3301 ctttcagttt tgaatcccct gcgtattctt ctgccaagaa acgacctagg cgtaccacac
     3361 ctgtagcgct agatacagtt tctaaacatc cgcgtttacc gcaagtacat tcgaaccctt
     3421 caggatccac agtgatatgt ccgatttctc ctccagctcc gctcacgccg tgaatcaaat
     3481 ttccgccagc aacgatacct ccgccaacac ctgttcctag agtaacgaat acaacgtctg
     3541 gatcgttttc tccagcacct ttccatcgtt cacctaaagc agcaacattc gcgtcattat
     3601 cgatagcgaa tttgattcct gttcctgcct cgatcagttt tttcacaaat tgaacttctg
     3661 tccagtttaa gttatacgca ccaataaccg ttccagcttc gctgtccacg cttcctgggg
     3721 tacccatgcc gattccgata aagtcttcgg ctttcatatt atataaattc aaacgatgat
     3781 tgattgattc aatgatttca ggaacgatat gcttaccatc atccaaaata ttggtatcga
     3841 tgctccattt ttgctgaatc tctccttctg gtgttaaaat tgcaaatttt gctgttgttc
     3901 cacctaaatc aatcccaata atcttcttgt ccattacaaa tttcctttct ttcggctttc
     3961 ttcaattcgg tgctctcttt ttaatatgcg ccacgctgtc atgtatgtat catgatcaat
     4021 caaacggcta tcatacagat tacgcaattc gatagtcatt aattcaatgt catagatgcg
     4081 tgcacccaca tagataaaga tgccaaattt tttgaacagc tgctgtacat catataagct
     4141 ctccatcttt aagcacttcc ttcatattat acaagtaaac catatttttt taaacccagt
     4201 aaaatacata ttacaaaaag aaatacaaaa actagtgtag aaagaatcct ttggtgtatc
     4261 gaatagcttg tctgttgttt cggtagaccc aaaacattcc ctaacactaa tccgccaatt
     4321 agcccaccga catgtcccca aacatctaca gaggtatcca gaagaccaaa aacaaatgtc
     4381 aacacaataa ataaaagaaa atgtctgact agctgctgga tcgctgggtt atgtttaaaa
     4441 tgaaacccta agataaacaa cgcaccgaac aaaccgaata tcgaagtact tgcaccacca
     4501 gacagaacat tcgcttcgtt aaaagcaaag cttgctgtat ttcccattat cccgctgaac
     4561 atataaatca agaaaaaacg ccaatggcca tagattgcct cgatttgctg ccccataaaa
     4621 tacagtatca gtgagttcat tacgaaatgc atcagtccaa aatggatgaa gattggtgtc
     4681 actaaacgcc accattcatt gaagtgaaca agataaggac catacattcc ggcataataa
     4741 gggatctcaa gtcgcggaaa taattccatc atgaggaaga ccgctgtctg tatccctaaa
     4801 aaagcatagg tccataacgg tcgtcttaac catgcacgca attttatttg ctgttgataa
     4861 ttcatgaaaa cctcccattt cattttagtc aataaacaat tgttggatct tctgatcaaa
     4921 gctctccggt tgccattgtt cattcaattg ttcacgaaaa acaaggctac atgttttttt
     4981 atgaaaatta tttaaatacc ggtcgtaata accaccgcca aaaccgattc gataaccggc
     5041 tctattgtag ataatacctg gtacaatgag gagatcgatc atctccggca aatagagctt
     5101 ctctgattgt ggctcaagaa caccaaaact cgaacgtaca agaacggact gttcatccat
     5161 ttcataaaac gccatctgac gtttcggcaa agttttaggt acaactattt tcttttgttc
     5221 tttttctgct cgttggataa ccggctgtgt atccagttca aattcattcg ataaagtcgt
     5281 ccctatcact tttgctgact tccagtactc gctggcgaat agctgtttat agattcgctg
     5341 ttctttttcc tttttcttgc cacttttggc cagatttttc agcttttcga ttcctttccg
     5401 gcgtaatctt actttcaatt ggtccaaaaa ctcacccttc cttctctcat aaaatggtat
     5461 aggaaaaaaa gaaaaatagc aatcgaactt tcaaataaat gcataaatag aataaaaaga
     5521 agacctagaa atgattggcc ttcctttttt aatgaatttc aactgttcag cgcgtgtttt
     5581 tgtctgataa aaagtgcata cagaaaatat ggcgcaccaa aaagggcaat caaaattcct
     5641 gtaggtactt ccccacttag tagaatcact ctagcaacca tatctgcact aagcagaaaa
     5701 atggaaccga acaaaccgct cagaacaaag gtccagttat tttctttacg tacgactagc
     5761 ttcgcagcat gaggagctag tagtcctaca aaagataaac tacctgtcac cgctacacta
     5821 ccgcttgcca aagcaacagc taacaacaaa aggatttttt gacttttagc cacgttaagg
     5881 cctaatcctt gcgcgcgttc ttgaccaaat gatagcaata caatcctctg tgcataaagt
     5941 cctcctagcg gaattaagat aaccgcccat ggaagcaatg cccaaacata aggccagctc
     6001 gcaccccaga ttgatcccgc taaccatgca ttcacaaatc cataattttc cttagataat
     6061 tggatcgtgc atagcaatgt taatgcagaa aggccggcat taacggcaat accggacaat
     6121 aagattcgat tcatacccaa aaagccattt cgatcatagg ccgcaagata gacacagcaa
     6181 gcagctaaaa caccacctag aaaggctaca actagcaata gaaacggcag cgaatagtgc
     6241 gaaaagaaac ctaaataaag catcactaaa aatcctgctc cggcattaat gcccaatata
     6301 ctagaatctg ccaattcatt tcttgttact ccttgcaaga tataaccaga aacgccaaga
     6361 caagctcctg ctaataaact gatcagtaat cgaggaactc gaaattctct aacaatcagc
     6421 gccaaagaag atgattcttt ccctatgaac gtggcaaaaa gctgatccag gtaaataaaa
     6481 ggcgtaccaa tcattaaact acctatagac aggatgacaa gtacaaaaag caagaaatag
     6541 aacgtccaat atattttttt caccgttgtt ccctccttac ttgtatcagt aaaaacggaa
     6601 cgccaatcaa tgcaatgact aatccaaaag gtgtttcaaa agggggattg atcatcctcg
     6661 ctgtaaaatc cgccaacata accaatccac cgccaactaa aaaagagaca ggtagaacat
     6721 aacgatagtc cgttccaacc attccgcgaa caactggcgg aaccaacagc ccaataaatg
     6781 aaacaggtcc gactaacgaa acggcaatgg ctgctaacac tgcaactaga cagaaaacaa
     6841 tcaaccgcgt tcgtttagga tgacttccaa gagctaatgc atgtgtttca ctcaaatgaa
     6901 tcaaagatat tgcttttcct aatgaaaaag aaaccaaaac agcaagtata tataaaggaa
     6961 acaatactgc taattgtttc catgtgatat tggcagctcc gcctacaaac caaaaagcaa
     7021 gatcctgctg cagattaaag aattgggtaa gtgcttggct gatagcagta aaaaaagaac
     7081 taatagctac gccagctaaa atcagctgta aaggattcat gcccattttc gaaaactgca
     7141 aaaaaccaaa tacgagcaaa gttacagcaa aagaacctgc caatgacatt agaaacacag
     7201 aaaaatcaga agctttaggc caaaaagcaa aactaagggc cattgccaac gaagcacccg
     7261 cgttgattcc tagtaagcct gaatctgcta aaggatttct tgtcactcct tgcatcaatg
     7321 caccgctcaa tgcaaaacaa ctccctacaa gaaatgctcc cgccacacga ggcaaacgaa
     7381 tatttcgtat gatttggtga ctttgttttg atctatcaaa atcaaacaac gcctgccaaa
     7441 aatctgcttt tcccacagtc gctgcgccat ttgaaagagc aacaaaaaaa ataatcaaaa
     7501 gaacaaaaca gaccaaaagc aggagtctat ttttcttcat agtcctcata attgatcctc
     7561 ctgctctttt ggcaacaaat cataggttag aaaaactggt ttatttgttc ttggaagcgt
     7621 gacaatcgtt gcatcaatct caaatatttt tttcatacac tcttttgtga tcacttcttc
     7681 aggtgtacct tctgtgatca acttaccgtt tttcattgct aataaataat ctgaaaaacg
     7741 tgaagcatga ttaatgtcat gaatcgacat caagatcgtt gtttgtttca tttgattgat
     7801 ttcagtcaat aactgtagga tctctaattg gtgtgccgga tctaaaaatg ttgtcggttc
     7861 atccaagatc aagatatctg tttcctgagc aagcgccata gcgatccaga cacgctgggt
     7921 ttgtccacca gacagcgttg cgatatcccg catacgaaat ccagctagac ctgtagcatc
     7981 caacgcccac tcaattgctt catgatcttg cgtgtccatg ccactgaaca gtttttgata
     8041 agggtatcgt ccgtaagata ctacttcttc tactgtcata cctgtagggt gttcactagt
     8101 ttgtgctaat aatgcaattt tttttgcaag ttcttttgaa ctgtatgtag agatatcaga
     8161 gttattgaca atgatctttc ctgatttcac aggtagtata cgggtaaaac ttttcaataa
     8221 agtagatttt ccacttccat ttggcccaat caaactagtg attttccctt caggaatcgt
     8281 aacagacatg ttttcaatga tttttttgtt ttcgtagccg gctgcaatgt cagctgctct
     8341 taatctcgtc atttcaccca tcctttcgct gaaaatcatt ctcaattatg acgctgtttc
     8401 aacatttcgt caagcgctac tttcatatct atagccaaat gaagcggttg acagcgttcg
     8461 aaatatcagg aataataacc aatgagaaag attctcaatt atgttaggag gaaagaaatg
     8521 aaattaaaaa caaaagtatt cacattatct agcatattgc tgctggctgg agtactgagc
     8581 gcttgctccc cctcttcaga taaagaaaca gctacaactg ccaattcgac gacttcatcc
     8641 aatagtactc atacagtcac agatacactt ggacataaag tagagattcc aaacgcgccc
     8701 aaacggatca tcggcagtta cttagaagac tatctgattg cactagggga aaaacctatc
     8761 gcccagtgga ctgttggcag tggttccatt caagattatc tgcaagatga tttaaaggat
     8821 gtcccaacga tttcttatga ccttccttat gagaaagtgt taagctttga gcctgatttg
     8881 ctgttgattt cttcttccgc tactgtcgaa ggcgggaaat acgaacaata tagcaaaatc
     8941 gcgccaactt atgttgtaaa aaacggaaca gatgtcacat gggaagaaca actaaaagat
     9001 gtcggcaaag ctttggcaaa agaaaaagag gcagaaaaaa tcatttccga ttatcaaaaa
     9061 tccatcaaga aaactcgaga agaactagca gataaaatcg agaacaagac agctgcagtg
     9121 ttgtgggtga ccaacaactc agcctttatg gtcagcaaaa atcggtcaag tggaagaatc
     9181 gtatacgacg atctggaatt tggtgttcct gatctagtgg aagaggtctc caaaaaagcc
     9241 acttctgact ggtctgctgt ttcctctgaa aagctagctg aattagatgc tgactacatc
     9301 atccttgtga acagtgacaa agatgctgcc atgttcaatg aaccaagctg gaagaatcta
     9361 aaagcagtga cagacaatca tgtactgcaa ttcggacctg aatctagttg gctatataac
     9421 ggacctattg ccagtcagga aatggtcaat gatatcaaag aaaaactaaa ttgattttaa
     9481 ctaaaaaaag catcttcacg attagtcgtg agatgctttt tttcttatta acaaactaga
     9541 cgcatagatt ttattcttct tttgaagcaa ccctattttt tactctttcc catttttgaa
     9601 aacgatgggc atatagattt tcttgatccg ttatcccctc atactcctct ataagggcga
     9661 agtctgaaaa atcaatattc ccaatatacg tatcgccttc aaacgtgcta tcgatcaatg
     9721 ttcgccagat ccgaccagtg taaggaagaa gttcttgaaa aatccgacta ccacccgaaa
     9781 catagatatc ttctgagatc gtttgactat acgacaaaag ttcatcgatc gaatgcatta
     9841 cttccgcatt ttctttcgtg cgaaaatttg gttgcgttgt caacacgatg atatggcgat
     9901 aaggaagtga aagatccccc attccttcat aggtctttct tcccatcact aaaattcgat
     9961 ccattgtgtg ttcgcgaaag aaacgcatat catttggcaa tcgccatggt aacaaaccat
    10021 ttttgccgat cagaccattt ttatcttgtg cccacatgga aataaacata ttatccgctc
    10081 cttatgattt tagatgtttt gagataaaaa aagtctcaat tctttgacca gcaaggaatt
    10141 gagaacttta aaaacattat tttgtttcac ggtgcaaagt aactttgcgt tcgcgtgggc
    10201 aatatttttg tttttccaaa cgatcaggat tgttacgttt gtttttgctt gttagatagt
    10261 tacgttcttt acaagaagta cactctaaag tgatgtttac gcgcatgttt ttccctccca
    10321 tttttacaag aatcctctaa gatgactctc agacttgatt atcttaccat gattttttct
    10381 agattgctag tgtttttcac gattttgtta agtaaaacta cttgacgata aatacaacgg
    10441 ttttacttct caaacaaaat ctttttcttt ttgtataaat acccttacac ttgtttatat
    10501 agattatgtt ttttaataaa gggggatact aatatgccat ggaacatgaa ggactttcct
    10561 gcatcaatga aaaacttaga taaattgaca agaaaaaaag caatcgacat cgcaaatgca
    10621 ttattagatg aagggtaccc tgataaccgt gcgatcccaa ttgccattga tcaagcaaaa
    10681 aaatggtatg aagatgctag tgaatcagaa aaacgaactt tcgaaaaaga aaaaaatcca
    10741 agcaagacgg atgaacacga tacgaatcct cgtgctggaa aactgcttga ttcagatgtg
    10801 atcgtagaat atgaggaaga acaatggatc gtgaaatcta aaggggcaaa aaaggcaagc
    10861 aaccattttg atacaaaaaa agaagcaatc gaaaaaggaa agcaagtagc acaaaacaaa
    10921 gaatcaactt tgttcattta taaaaaagat gggacaaaag aaaaagaaat cgattattga
    10981 acatatgtaa aggggttgtg acgaaaatcg tgtcacaacc cccttatgtt cgaaacaatg
    11041 gtgttcctct gccaactccg aaccataagt cataatatct ataaaacggg aagagctgtt
    11101 ttttgaaatt gtttcttatc actcagtgtt aaacggtagg agaaaacctc ttttttattg
    11161 atatttgatt ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg aatcgtttca
    11221 tctacaaagt agactttata ccatgtcaag aaactgaaga ttgtccagat ttttctttgc
    11281 tcttctttac gtccttcgtg atggtcatct agcaacttca tgattttttc ttgatcaaaa
    11341 aattcttttg caaaatctgc ttcgaataat gctttgactt gttcgtagcc gtgttcttct
    11401 ttcaaccaag ctttgattgg cacagggaat cctaatttca cacgattcga ccattcttcc
    11461 ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg attcaataaa
    11521 aacttacttg gtatttgttg agccaatttc atgacttcga tatccaataa aggtactcgg
    11581 acttccaaag aacttgccat agaaagttta tctgccttta acaagatatc ttttggcatc
    11641 cattgatgaa gatcgacata ttgcatctta ttgacttcat ctttgattcc ttctgttttc
    11701 tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg tttgatattt tggtgttaac
    11761 acttctgatg cttctgattc ttcgaacact cgagcatgac cgataaaata ttcttttgca
    11821 ggagcaagtg attcatataa atggatccgt ccatggaaat ttttcatttt tccgatttta
    11881 cgtcctaaat tatagcgtgt tccttttggt aattttttca atccttgagc aatcacacgg
    11941 atgaattttg aattcgtatg gaatccatac gtttggtatc ccgcaaacag ttcatccgcc
    12001 ccttctccag attggactac ccgcacactt tgtgaagcaa gatttgctaa aaagtacaaa
    12061 ggtacgcaag aaggatttga atcaggttca tctaagtgat actggatcaa agggaatgct
    12121 ttgaacgaca tgtctccgtc gatcactgct gctgtattat ctaagtccaa tagttcggtt
    12181 agttttctgg cttcaatcgc ttcattatag gttttatcat caaacccgat tgagaaagaa
    12241 tgatctggtt tcaatacaga ggttacataa ctggaatcaa ccccgctgga taagaaagaa
    12301 ccaacttcta catcactgac cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt
    12361 ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc atcccaatat
    12421 tgctgaatgt ctatttttcc atctttatac gtataataat ggccttcagg caaacgatag
    12481 acatctttga agaatgtttc gccgtttaat ggagaatatt ggaaagtcat ataaggtttc
    12541 aacgcttctt tgttcaattc tttgttaaaa tcaggatgag gcagaaaact tttgatttct
    12601 gaaccaaaca taaatgttcc gttcatttct gcataataat atggtttgat cccaaagtgg
    12661 tctcttgcac cgaataactc attcttttca ttatcccaaa tagcaaaagc aaacattccg
    12721 cggatctttt gcaataattc tgttccccat tcttcgtaac catgcaaaag gacttctgta
    12781 tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt cttctctcaa aggctggtaa
    12841 ttatagattt cgccattaaa aataatgatc tttgtccgat cttcattgta aataggctga
    12901 gtaccgccct ctaagtcaat aatacttaat cttctgaatc ctaaagcaac atgtttatcg
    12961 atatattctc ctgaactatt aggtccacgg tgaacgattc gatccatcat tgtattaata
    13021 attgtttttt tgttgtcctt gtcgttaaca aaacctacaa taccgcacat ttaactttca
    13081 catcctaata taaaattact gtttatttca ttcatatgca tcttatcata gaataaatag
    13141 cggacgaata ttttctctct tacaatttcg tcacgtgaaa acaaaagatg aaagtttctg
    13201 tttttacaga aaataaagtc ccaatatcta atttaccatg ttttcttttt tgaggctacc
    13261 ttattctctt cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa ccagcaaaaa
    13321 aagacacgac ctaaagatcg tgtctaatta tgactgtatc agaaaatatg ataccaaacg
    13381 gatctattca ttaattaatt gacatagttt atctgttgct cttttatgac tggataactg
    13441 accaattcat gacgatccaa gtcttcttta ttcatattta cagcagtaat ttgactgtct
    13501 tcatacgtac gataataata aatcccttta tcaacgttac agcaagaaga ataaatcgta
    13561 tattcgtatt ttctttctcc agtatcgcat actccttttt gttgctcgac tgagcctagt
    13621 atatgaaaaa attgactgat actttcagat tctgaatcac ctgatgcagc attcatttta
    13681 gtaaatgtgg cttttacaaa tcgagaaaca gaagagagat ctccaggtaa gcccaatcct
    13741 cccatcccac gactataaac attcaagctg atctggtttg aaaaatgatt ttctggtgtt
    13801 ccatttgata gcgagcgata attgttcaga ttaaataatt gataatcaaa tggcggatta
    13861 tttgtcaaca caccgacagg attatcatat aaatgaagtc catctttcat actttcaatt
    13921 actatcgatt tttctttatc agctaataac cagtgtaaag gtgacaaggg tagtttttca
    13981 ctaaaatcga tattgactag attgatagta gccaataatt ctttagcttc gtctatggtt
    14041 gagcattgcc ctagtatcca tggaataaac tcaaatggtg caacgttatc ttttccttct
    14101 tgtaattctt tataatcagc attgccagga aaattcagtc ctgccatact caaccctttt
    14161 tcatttgtag cctcataata gagtggatag ttggataccc cagctgctat tcctatcatt
    14221 gcataatgtt tatctaaatt ttttaccttt ctaaaatcga aacgataatt tcttggagta
    14281 atagtgacca cttcattata agacatctca taatcaaaat tccttccaaa ataatggt
//
LOCUS       DAGCWU010000061        14143 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.061, whole genome shotgun sequence.
ACCESSION   DAGCWU010000061 DAGCWU010000000
VERSION     DAGCWU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.061"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1092)
                     /locus_tag="MBZ71_001453"
     CDS             complement(<1..1092)
                     /locus_tag="MBZ71_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBT4429414.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPY"
     gene            complement(1089..3278)
                     /locus_tag="MBZ71_001454"
     CDS             complement(1089..3278)
                     /locus_tag="MBZ71_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4429415.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(3412..4602)
                     /locus_tag="MBZ71_001455"
     CDS             complement(3412..4602)
                     /locus_tag="MBZ71_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HBT4429416.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(5474..7105)
                     /locus_tag="MBZ71_001456"
     CDS             complement(5474..7105)
                     /locus_tag="MBZ71_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBT4429417.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(7119..8792)
                     /locus_tag="MBZ71_001457"
     CDS             complement(7119..8792)
                     /locus_tag="MBZ71_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBT4429418.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(8805..9122)
                     /locus_tag="MBZ71_001458"
     CDS             complement(8805..9122)
                     /locus_tag="MBZ71_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBT4429419.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(9147..10547)
                     /gene="lacG"
                     /locus_tag="MBZ71_001459"
     CDS             complement(9147..10547)
                     /gene="lacG"
                     /locus_tag="MBZ71_001459"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBT4429420.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10784..11569
                     /locus_tag="MBZ71_001460"
     CDS             10784..11569
                     /locus_tag="MBZ71_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4429421.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            11572..12264
                     /locus_tag="MBZ71_001461"
     CDS             11572..12264
                     /locus_tag="MBZ71_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4429422.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(12538..12909)
                     /locus_tag="MBZ71_001462"
     CDS             complement(12538..12909)
                     /locus_tag="MBZ71_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBT4429423.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(13309..13539)
                     /locus_tag="MBZ71_001463"
     CDS             complement(13309..13539)
                     /locus_tag="MBZ71_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429424.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(13633..13971)
                     /locus_tag="MBZ71_001464"
     CDS             complement(13633..13971)
                     /locus_tag="MBZ71_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429425.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT         4361 a         2890 c         2279 g         4613 t
ORIGIN      
        1 atacggtaat tctttcttta aatactcacc cagtgaaaac agaggatagt gttgaggagc
       61 aagtacacaa atatcttttt catgaatgcc gctttcaatc ttttccttta ttatattttt
      121 tattatgttg ggtaaatcct ccttagtaac tttgcaatca taacttataa aaccacggtc
      181 gtttttatta ttccctctgc tttcaatctc gtaaggacaa ctctgaaaat tagagtagaa
      241 gtctactaat ctctgattag acctgtaaca gccatgtagc gtctttgatt gaaaagtgtg
      301 actagttagc atttctagtt cttcaactga tttagctata ccatctaatg atccataaat
      361 tgcttggtca atatctccaa caaacattga ctgaatttga gggttgtttt gaagtaattt
      421 agctattaca ttgtattgta attctctagt atcttgaaat tcatccacat aaatcgccct
      481 tatattacta gcaatattac gtgcaacgtt actgtttaca gttaaaattt tatacgctac
      541 tagtaaaatc atatcaaaat caatttcttt atttttttcc aacatagtgt gatacatttc
      601 agaaacttga gtatactgaa cttcattaat tttaaaatca tgagaaagtg acaaatcaat
      661 tttgttcagt tcatacgttt tgagatttaa attcagcata ttattaattt ggctgacgta
      721 agctctttga gtataatcat caataattgt aaatcctttt gatactcttg agagattcat
      781 tttaaaagga taaataataa attcaagaca aaactgatgt attgtcccca cccaaatatt
      841 aatatcatca tctatactaa gtaaacttag gcgttcctta atttcttcag cagctctatt
      901 agtgtatgtg atagcaatta ttctttttag tgatccatcg tgtttaattg cttggtaagc
      961 aattttcctt gtaagtaccc ttgttttccc actcccagga catgcattta cataaaggtt
     1021 atcttcactt tccacaacat ctttttgttc taaatttaaa tcatttacga ttttttcgaa
     1081 gatatttgtc attttgaaac ctcagcataa agtatttttg tgtaattttc tatctgattg
     1141 attagataca caacatcctc atctttacca aagtactcta ataaagaaat tattgctata
     1201 ttcaaatcag ctgtatcact tgtttttaaa ctagctgata aagctgacaa ttctgtagtt
     1261 tcctcgtaca agtcgattgt atggagtaga atttttttat aaattgagag atctactatt
     1321 tcctgacatg caaaagcaat agcattaaga atatacttag gaattttgat taaaaagtca
     1381 attttttcac ctaactcaat tgcaaaccac cccttaccaa catgattggc tagttttaac
     1441 atcgctaaat ttctttctaa atattgttca ctttgtattt cttctttata agcagttatt
     1501 gtttttttat ctttgaatgc gtcatctata attggactga cataagttag aatattttct
     1561 ttattcacat ctaaaaactc aatttcaaat gttgtattag cataaaaaca attaacccaa
     1621 ttgttttcat taaaaaggga ggataatttt tcttttcttg cttgaccttt ctcttgtgca
     1681 gtgcttttat acaatcgatg ttctgatggg attgcctgtt catctaaatc agttacaata
     1741 gcacactttc tctgtattct atcatcgtca aacaagctag cgatatattc aaacgaagta
     1801 ctaccaacat taatcaaacc tattccaagt tcgtctaagt ttataccaaa taattttttt
     1861 actatttgag gaataagtat ttcttcagca tctccttcaa ctaagataac acctttagaa
     1921 aaaagcaatc catttctttt aacatctaaa tatctctcta ctgcgtttat tagatttaga
     1981 ttgttctttt ttaacttttc tttagcaaat gagttcagat tatttgctgg attcataacg
     2041 atagatttgc cattaaacga ttttaataca ttcatccgag atatttcaga cacttcagct
     2101 aaatgtacag aatgtgtggt catgagaatc tgagtatagt ttttttgaag atttaatcct
     2161 tcgaacagag ttttttgaat atgatgatga atatgagctt ctggttcttc aatcaacata
     2221 atattaagta gttctcttga acgacacgct tcaaattcga ctattttcaa agctaaataa
     2281 atcatattta gatgtcctaa ccctagtaag tctaagtcga tattttgttc tggcttcatt
     2341 gaaagaaatt ttgctaaaga acccatatca tctgacatag ttgaggaaag aagaagatta
     2401 ggagaataca ctgaaccaat tatgctattt agttgccgat taatattttc gccaattttc
     2461 ctaatttcag gaacactggt aatagtctca tttaattgct gtataattgt ttttaatgac
     2521 tcaacattat ctgatgatat ttttgattca attgtttcca tgattctttt gactgggttt
     2581 cgattgtttt tcatttctct aagaacatct cgaagagcgt caacataaac aaccgaaata
     2641 tgcttgaaaa tatctgacat gtcaattctt gagcccaaca gagcttcatc gtcttcttca
     2701 ggattactag ctatattgtc attgagtctt cctaccaatt tatcataatt ttcattgata
     2761 cagaaattta gatttgattt agatgtgtag taaaattcat agtcggatag acgaatagaa
     2821 tttctaaatt cgttaaattg atgagagtct aattcagtgg aaatatcaaa taattgcttt
     2881 ctaatagctt tgttaggtct aataaataga gaaatactac ctacaccttt atctgcattt
     2941 gaaatcaatt cttctaagat tgtttgactt tcagaattta aagaaatgga agcacaaatc
     3001 tcattgtcaa attcttcttc gctgattcct tcaaatgtcg cagagataat aatccaatgc
     3061 ccttgccacc cattttgaat tccatgaaaa aaatctgatt cttttagtgt tttactacta
     3121 tagtaataac tatcatctaa taagattctc aatgcagtaa gagcattggt tttccctgaa
     3181 tcattctcac ctataatagt atttgtctca ttatcgaatc gaaagatttc ggattcataa
     3241 tttctaaaat ttcgaatttg aagagtagat ataaacataa gcagatctcc taactattta
     3301 attaatatga ttatatagta agtttatcat aaattatgtt agtaaaagtt ctcattataa
     3361 gggagcgtca ataattttgt gtaaatgatt cttccctact gcagattgtt tctaattctc
     3421 aatcagttgg cttagcattt gttcgagctc gccttcggcc tgttggaaac ctttatgact
     3481 gcgacctagg aatttttggt tgtaagtatc aaaagacgag actaagaaac gttccatcga
     3541 ctcttcattt tggaactgtt ccttgcggtg gctgtatttc ttgatttgtt tattaaagga
     3601 ttcaatcaag ttcgtagaat aaatactctt gcgtatggcc aaggggaaat catagaaagt
     3661 gagcaagtga tcgttcgaaa gaatcgattc aaccactttt ggatagctgg ttttccattt
     3721 ttccgcaaaa gtaccacgtg cttccaacgc cacctcttta gatgaggctt gatacaccat
     3781 tttaaaatca tcacagactt ccttacgatc atcgacacgc actttatgac tgatattacg
     3841 ggaaacgtgt acacaacaat gttgaaaacg agctttggga tagaaccgac taatcgctcc
     3901 taccatcccc tttaagccat ccgtgatgaa caggaggaca tttttcaaac cgcgctcttg
     3961 aaggtccaat aaaatttcct cccaaatcgt gatggattca gtcggtgcaa tcgcatagct
     4021 caatacttcc tttgatccgt ccgggcgaat gccaactgcg atatgaatag cttccttggc
     4081 gacggttttc cgctttaacg gaatatacgt tgcgtccata taaatagcag cataacggtc
     4141 atgaagctcc cgcccattaa acgccgttac ctcttctgta aatgattttg ttatattgga
     4201 catggtttgg ggcgtgtagt aatgcccata cattttctca atcaagtctg cgatttccga
     4261 catggtaata ccttttcgga agaggtgaat gacggtctcc tctaacgtgt cattcgtccg
     4321 tctataagca ggaacagtct gttgcttgaa ctcaccattg cggtcgcgcg gaatctggag
     4381 atgaagttcc ccatactcgg tcttgaccgt acggtcatag gagccgttac gagaattacc
     4441 cgtgttaaaa ccaatgcgat catacttttc gtaatctagg aaagccgtta actccgtttt
     4501 gagaagcgta ttgactgctt tctccaaatg gacacggaat aattcattca aatcgccttt
     4561 gttcgctaga gtctttagaa tttctgtagt aaaatcgttc atagggaagt cctctcttct
     4621 gtgaatgtgt tgtggtaact ctattctaca gaagggactt ccttttttct atttacacaa
     4681 aatattttac actctccatt ataacaattg ggttaaaaag aaaatagata gtattctacc
     4741 tcatatttat ttgaaagtaa tatataaaag acgttcaaat tcgaacgcct ctcgttaacc
     4801 aaatattgga ttcagaatct agttttcata tataaacaga gtatgattag gtgcaaaaat
     4861 tgcactttta ttgcactttt atttttattt atagtgtttt actgttctgt atattgtttt
     4921 aactcccttg atagtatcgc attctgtttt atacacttta cgttgaaaac agagtgggaa
     4981 taaagagcca acataatatg gagggggaat ccctataaat caacattctt caatagctaa
     5041 aatgcttgat tttcattaaa aataagatgg ttccctacca aaagagacta taatataagt
     5101 taatttgttt cattcttatc ataaaaagaa gttctcgaaa aacgaactgt tttccgagaa
     5161 cttttttcta tccatctaag tgctcaaatc atattcacga cggaattcta aggttaccct
     5221 ttcttaaaca aactttatgt tatctgcaca actttttgca attttttcat tgcttcttac
     5281 gaaatcgttg aactctcttt agcataatac aaagtgtaga tgaagtctgg tgctttactc
     5341 gttttggtaa aaatgactgg aaatcgtttc tggttatttt tggcaaaggc gaggtgcagg
     5401 ttcgattcct gccttctacg ctaacgatgt ggctgaaggg aacctccgat cttgaatcga
     5461 aacgatccgc agtctattgt ttcaccaatg aaacaaaatc aacgttcgtc caagcatttc
     5521 ctgaagcctt gctataaacc ccaatttcac aaacacctgt tgtaattgga atgtctttga
     5581 ttgtaatttt tgtccattca cttgttttaa caaccgcagt atttagttcc gctccaccat
     5641 aatttttgat atataactga ttagtatcaa actctccccc agacattaca taggcactca
     5701 atgtgtaagt tcctttgctt atgccactaa cattttgata agctgatgcc tcgtatgctt
     5761 gatcctgcca aaaagttagt ttgtagtccc caaaaaaagt gcccgcttcc gttttgatcg
     5821 tacccttagc ggttccttgt tgaaaccagg tttgccaatt ggaaggactg gtcgttggtc
     5881 catcgctttc gaaacttgca tttttaacta gatttatttg cgcccctgct tgtttaaacg
     5941 cacgcaagct tggcaaggcg tttccagcaa aatcaaacat cgcttggttg tcccattcgt
     6001 ttcccatagt ggattgaaca ctgagataat tcatacctgc ttgtgttgcc caactcacat
     6061 ttccgttgta ccaaagaggt tcccaataga agaagcccgc cccgcgatta ttcggtactg
     6121 cctgaatccg atcggcaaga tccagcaaat actctgcttg tcctgtcggt gttggttcat
     6181 atccccccgc agctgcttcg gcttcaccaa aagcatttgg catagtatcg gcattcgctg
     6241 ttgtatgccc ataagctgtt tcaactacga tcacttcttt accataccgt tggctgatcg
     6301 cattcatatt ggtttgaagt tcattcattg aaccgtgcca atacggataa tacgacactc
     6361 caattaaatc aaatgacaca cctcgactcg tgatttcatc aaaccaccaa gtaaatgctc
     6421 ctgtatcgcc accatcagct aagtggagca ttactggagt tgttttaggt tgagtgtctc
     6481 gaacagcttg gataccagac ttcaaaaaag cagccaatcg atcaaattct cccccgcctt
     6541 ctccccaaga cttgccataa ggccagagca ttccgctatt cagttcattg ccgatctgaa
     6601 ccatatcagg ataaacgtcc agctgcttca tttccgacag aacgttcttt gtatagtcgt
     6661 acaatgccgt attcatttct tcaaaggtca gaccttgcca tgctttagga agattctgtt
     6721 tcccaggatc aacccaaaaa tcactgtagt gaaaatccag cagcacctta agtcccagat
     6781 tttttgctcg ttttgcaagg tcaatcgtcg tttccagatc atttgtccct gctccataac
     6841 tttctccatt actattataa ggatcctgcc acaaacgcag tcgaacatag ttgacaccat
     6901 tattttttaa aatctctaag gcatcttttg aaatatcgtc ttcataatat tttgcaccag
     6961 atttttccat atctgacagg atcgaaatat ctgctccgcg aataaattcg ctggcagatg
     7021 cttcaacagt ttttgaaaca gcccccaaag cccctaacac agctgcaaat aaaacagctt
     7081 tacgcaacca aattcccatt ttcattatgg ctcctccttt attgaaattg gttcagaata
     7141 aactgaacag ctttttcagg atctctcgtt aatccgatat attctgctcc ttttgtcgca
     7201 accagcttga ttcctaagcg atcggtgtct tctttgatat catcatagta agaattgact
     7261 tgcggtgcta agaccaccat atcaaaatcc tgcagcattt cgtaatgaga accataagca
     7321 cccgccattg cactgatcgg tgctttcgat tcagatgcac ctttttttaa agcattagct
     7381 aacatcgcac ttgtccctgc gccagcacaa agtaccaaca cattgatttc tttatttgca
     7441 tcgctgataa cttgactcgc tgccacaggt acttcctcac gtacatcggc agaagcacct
     7501 tcctcggttg cgatcacctc tgctttttgt gcttctttct ctactaagac agcatcgtat
     7561 gctttgcaaa atggtagata gatcaggaag tcaacgacga ttagaaccgc tgctagaaca
     7621 aaagctaaga tacccatgcc cgttcctaga accaaaccga tcggtgctgg agttgcccaa
     7681 ggcaaaacgt acataaagct gttcatgccc aatacatcga caaaaaactt gaacaggcaa
     7741 acgtttacaa caggtgttaa caaaaatgga ataaaaaaat aagggttcaa aatcatgggt
     7801 gccgcaaata gcaatggttc gttgacagcg aaacttactg gaataaagga agctttccca
     7861 actgccttca actgcttaga cttagcaaag agtaggaaga taaaaggaac aaccaatgtt
     7921 gccccagttc ctcccatagt tcctacaaaa ttccctaaac ccaccgtcag tactttagaa
     7981 gcttgttcac cggaattgaa caattgcaga ttggtttcca cattggcata gataattgcc
     8041 gcaattgccg gctccacgat cgacggtcca tgaacaccga caaaccaaaa cagcgccatc
     8101 gctccccaga taatagcgat tcctaagtac ccgtcagctg ctgtaaatag cggctgcagc
     8161 accgtaatga ttgcttctgc aaatgagatt tggaagattt gtcgaatgcc taaatcaatt
     8221 aaaacggccg caaatactga aaaagagaaa ggaaaaatat ctcggaaggt ttgcgaaatc
     8281 gttccaggaa cttctttagg cattttaatg gtaatatctt tcagcacaca aaacttatat
     8341 aaattaactg ttaaaaatgc agcgacaaag gaagccagta gtccctttgt ccccatatat
     8401 tccgttgaaa atccaccttc aatcggagag acgcttaaca acaggaaact taccacagag
     8461 gccagcatca cagaggtggt gttgattact ttgtttttcg gtaagcgacg attcatcgaa
     8521 tcagccaagc agcgagcagt ggttgctgaa acgagtaacc ctacaacacc catcgaataa
     8581 ttatagactt tccatagcca tgtggagaca gattcaggcc attggtaacc aaaaatctca
     8641 ggaaccgcag caatcaaaat gaaaatgctt gaaaataaaa tagctggcat cgctgttaaa
     8701 aatccgtcac gaacggctcc taaataacta ttacgtgaga ttttttcaaa aaatggtttc
     8761 cctttttcga tttgtttaat gattccatcc atagcgttct tctcctatcc ttgattctgt
     8821 ctgtacagta caataaagtc ttgaatcaga tctctcagca ataaagtggt cattaaatga
     8881 tcctgcccat ggataaaaat gaatcccatc tccatctctt tcccagatgc ttcttccgct
     8941 aaaatttttg tttgcgaatt atgtgcaagg gttaaatttt cgtcggcttc tttcaacgcc
     9001 gaatcaacat gttcaaagtt tccttgtctt acttctttca ataatttcaa taatgtactg
     9061 cgagcgtcgc ctgaatatgc aacgatctca aaaccaagca tttgtaattc ctctttattc
     9121 atcttttctt cctccattat cacttattaa ataatcgttt tggtttcact gaccaacttg
     9181 taccagtaag ctgattcttt tgggtaacgc ttctgggttt caaaatcaac ataaaacaag
     9241 ccataccttt tgttataccc atttgtccat gagaaaagat ccataagcga ccacaaaaaa
     9301 taccccttca catttacccc ggctgttatc gccttgctta aggattccaa atagactctc
     9361 aaatagtcga ttctcggctg atccataata atgccatctt caaactgatc tttatacccc
     9421 atcccgttct cagtgatata gattttgttg taatgtgggt aatcgctttt aattcgcagc
     9481 aacaagtcat atagaccttc tggataaatc agccagtccc aatcagttcg aggaattcct
     9541 tctttataaa tacgttctcc gattcccttc actttataaa cagaggtccc tttttcaccc
     9601 gtaccgttat gatggattgc attttctcca tcgtaagctt tgacaaaatg gcattggtag
     9661 tgattgatcc ctaagtagtc attacgcgtg gatgcttttt tcaattcaac gaaatcttct
     9721 tcaggaaaat gatagctggc ctgatttgct tcacatatct catcaagagc agtcaaggtt
     9781 tcagttgaat aatagcctaa ataggtagca tctaataaga aacggatcga caatgcatcg
     9841 tctaaaaagg cagcatgctt atcttccggc gcgtctgttg ccgcatattt cgtctctaat
     9901 gagtgaacta caccaatttc acccggaagt tcgttttctt tgaaatagtt cactacacgg
     9961 gcatgagcaa ccatcatatt atgaagacag gcgacgatct ttgtaaaatc atatttgatt
    10021 cctggtggga aaacacctaa caaatattga ttggttgcaa ctggatagat ttcattaaag
    10081 gtactccaca ccttgacttc tttgaattca tgaaaacaaa aaatggcata ggaaacaaat
    10141 gcttctattg tttctcgatt caaaaaatct ccatggtcaa acaaacgttt aggggtatca
    10201 aaatgatgca acgtcacaaa cggcgttacg tttcttttct tacattcttc gaacacacga
    10261 tgataaaaag cgattccttc tggatttggt ttcccagctc catcaggaaa aatgcggctc
    10321 caagcaatgg ataatcttag accatttaca ccaaaacgtt cacacaattc aatgtccttt
    10381 gcatattgat gataaaaatc acttgccgga tcgggactga atcgcccttg ttcctctaaa
    10441 aagtcatccc aagcaacagc gcctttgcca ccttccttag tggctccttc aacctgatag
    10501 gctgctgtgg ctccgccaaa aataaaatct tctggtaatt taagcatgta catctctcct
    10561 caaatctata atcagttttc gctctttcta actgattgca ttgtaaacgc atccaaaaca
    10621 tttgtcaaca aaaacaaaca caaataatca taaaaaccag aaaacagcaa taaaatctat
    10681 tgtaacaaac ataaaaacca ccctaaatgt ttacttttgt tcgaattaga ttgactgttg
    10741 tccgtttata tgatagatta tccttaaatt agaggtgaag acaatgttga aaaaagaaag
    10801 attgcttact atcgtagaaa tggtcaataa aaaaggaatc cttaccgtta atgaaatcat
    10861 caatcaatta gatgtttctg atatgaccgt tcgcagagat ttagatgaat tagaaaaatc
    10921 gggcaagctg ctgcgtgtcc atggaggggc tcaaagtctt tcctacactt tagatcaaga
    10981 actttctcac actgaaaaag ccactctgca gattcaagaa aaaaaagaaa ttgctgccac
    11041 agcagcctcc ttgattcacg agggagaaac gattttctta ggaccaggta caacaatcga
    11101 actattggcg acgcatttac tgaacaagaa aattcggatc attacaaata attatccagt
    11161 ttttgatatt cttgctcaag cagatggacc agaaattcta ctgattggcg gtgatttccg
    11221 gaaaaatacc ggcgcatttg tcggaccgat caccaacgac aatttaagaa gactaaagtt
    11281 tacaaaagca tttatcagtt caaatggcgt tcacaacgaa gcgatctcta cttacagtac
    11341 cgaagaagga gaagcacagc agatcgctct aaataattcc cgtaagaaat atctactcgt
    11401 tgataataaa aaattcaatc gagatgattt ttatgtgttt tataatttgc atgatttcga
    11461 tctgctcatt acggatcgcc agatctcgcg ggaggttcaa gagcactatg cacaatatgt
    11521 cgatctgcaa attgctgatt caaccgaaaa taccactata ggagcgtaac tatgaaaggg
    11581 attatatttg atttcaacgg aacaatgttt ttggactctc atctgcatga agctgcttgg
    11641 ctgcatatga tacatgatca tacaaaagat ggtttatcag acgaggatat tttaaaaaat
    11701 attcatggtc ggacgaatac ggagattttg aaacatttca tttcagagca tttgaccgcc
    11761 tctgagatcg ccgtattgtc agaagaaaaa gaaagctatt atcgtatgct ttgcttgcaa
    11821 aatgaaaacg agcttcagtt gacaaaaggg cttgaagcga ctcttgacca gctgactgac
    11881 caacagattc cattaacgat tgccacggct actgtcaaag agaatgttgc tttttatttt
    11941 gatattttcg atttagcccg ctggtttgac tgggacaaga ttgtctatga cgacggctct
    12001 ttccctggga aaccgcaacc ggatattttt cttaaagcgg cagacaaact gagcttagct
    12061 ccggctgatt gtcttgttat cgaagatgct tactcaggat tacttgctgc caaacgtgcg
    12121 gggatcggaa caattattgc aattgatcca ttcggtaaaa acaaaacagt atttgaacag
    12181 gcacagctaa ataaagatgg aaccatcaaa gattttactg gtttttggac agagcacctg
    12241 tctgccacat ccgttaattc ttaaacgatt caactaacta ccaaagaaaa gcaactgaaa
    12301 tgcacacctc cttttataaa aaactgccag tgaaacgaca tgaaagatac actgctatat
    12361 ccttagtgaa atatagcagt gtatctttga ctttttcatt cttttaacca tatgacgcgt
    12421 tcatctctat ctataattaa cattaaaaat agtgttttca cattatttct caaaccaatc
    12481 ttaaaaatca tcaggagtac ttttttgata tacttttatt aatgctgggc gtaatggtta
    12541 aaagatttcc actagttcct ctccaccaag ctgagttgaa aaatgcttat ctgtaaacaa
    12601 catagacaaa gaggaaataa acgtttccgt atctttctct gtcccaagga tattaattaa
    12661 ttttcctact aggtaagtat cattaaactt aagatctgcc ttcacatcaa aagttttgat
    12721 aaaagctttt atcatgcgta caattttgtc tttatcgcca taattgattt caccactcat
    12781 atcaatatag tcactaatcc ctgaagaaat aatggcaact gcttcttctt tcgccttatc
    12841 agataattca tttaatgact gggattgatt gatgacttct tgagttttat ctgttacgta
    12901 atctctcatt atcctatcgg catactccgc aaagtaatct tgaaaaaatt cttctacagg
    12961 gtaaccaact tcgttttccc aaccaggaga aatttcatta tcagtactta ttaaatctat
    13021 taaaaaccaa cccgttctct gagtgttgat tctataatat ttttaatttc ctatataaat
    13081 caaaaatatt atagaatcaa taagctaatt aagaaacaaa ctttagaaac tctagaagaa
    13141 cttctgaatt tatatagaag tgtgtagcta cttactaaat atacaaaagt taaaataaaa
    13201 tactctggat actctttata ctgcattaaa gttagtaata ttaacagcaa agatatcgaa
    13261 aaaacaataa cataatatgt gtcaaaattt ttcataataa gaactccctt actgttgcat
    13321 actaacataa tccaaaaact cttctacttc agctaaagac attccacttt ctttagtcca
    13381 aaaaacagtt ttttctatcg atccttctct gtaaactgta aatgtcggag tcgagttaat
    13441 tgagtagcga ctcttgaatt ctttccactc attttcatct tctctaactt ttgctacatt
    13501 cacgtaataa atattttctt ttcccttttc taccagcata tcgaatagct tcctgctttt
    13561 gatgatcgat ttcttgtgct acatctagta aattttcatc aatttctata tcataaagta
    13621 tttcactagt gtctacatcc acaatatata ctttttatca gtagcaattt ttgagtaatc
    13681 cgttaaaatg atctcagtct cgtcactttc atcagtataa atatacgtta acaatgaact
    13741 caccttttca atcggtgtta gctctaagcc tactgtattt tttactattg agtcattatt
    13801 taccaccata acttcgtact gtatatacca tcctatttgg tttccatcaa tatcagtaat
    13861 tggctcacta gctaatggag tctccggaac aatattttcc tgatatttct taaactcttc
    13921 tgtagcacga aaaaggttat catgttcctc acttgaaact ggttgagtca tagaaaatgt
    13981 tacttggcta aacagaggga aaataaacac tacaatcaac aaagtataaa aaaaagccct
    14041 tctttttcat tttttatctt ccaatcacaa attgaaaaac ttattcctgc aactttgagt
    14101 ctagtatcac aatcgactga ctacaactat tcatttacat tct
//
LOCUS       DAGCWU010000062        13886 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.062, whole genome shotgun sequence.
ACCESSION   DAGCWU010000062 DAGCWU010000000
VERSION     DAGCWU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.062"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(176..1543)
                     /gene="nhaC"
                     /locus_tag="MBZ71_001465"
     CDS             complement(176..1543)
                     /gene="nhaC"
                     /locus_tag="MBZ71_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBT4429426.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1652..3073)
                     /locus_tag="MBZ71_001466"
     CDS             complement(1652..3073)
                     /locus_tag="MBZ71_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4429427.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3316..5193)
                     /gene="tdc"
                     /locus_tag="MBZ71_001467"
     CDS             complement(3316..5193)
                     /gene="tdc"
                     /locus_tag="MBZ71_001467"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBT4429428.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5484..6740)
                     /locus_tag="MBZ71_001468"
     CDS             complement(5484..6740)
                     /locus_tag="MBZ71_001468"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBT4429429.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6852..7057)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7320..8621
                     /locus_tag="MBZ71_001469"
     CDS             7320..8621
                     /locus_tag="MBZ71_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBT4429430.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8690..9289)
                     /locus_tag="MBZ71_001470"
     CDS             complement(8690..9289)
                     /locus_tag="MBZ71_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4429431.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9543..10424
                     /locus_tag="MBZ71_001471"
     CDS             9543..10424
                     /locus_tag="MBZ71_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429432.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10437..12047
                     /locus_tag="MBZ71_001472"
     CDS             10437..12047
                     /locus_tag="MBZ71_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBT4429433.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12124..12783)
                     /locus_tag="MBZ71_001473"
     CDS             complement(12124..12783)
                     /locus_tag="MBZ71_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBT4429434.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13066..13731)
                     /locus_tag="MBZ71_001474"
     CDS             complement(13066..13731)
                     /locus_tag="MBZ71_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBT4429435.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         4065 a         2810 c         2547 g         4464 t
ORIGIN      
        1 gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaga
       61 agcaactcct tcggaaataa gctgaaattc acaaaaattt aaaaagcaat tttcttgagt
      121 ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tcttttttcc ttccttcaat
      181 tttttacacg tcgacgattc aataaaaaac cgcctaatat tgtaaagaca ggtgcaagaa
      241 tcgagaaaaa ggcgaatggc aagtaatgca acgcgctaac tttcatcgtt cccataatga
      301 atgtgccgct gactccccac ggtacgagag aattgacggt cgcacctgaa tccgctagtg
      361 ttcgtgtcaa ataggttttt gataagccaa cgtcatcaaa agaacgcttg aatgtttctc
      421 ctggaagaat gatggacaga tactgttctc caacgatcag attgattccg atacagctca
      481 aggcagtaaa cccgactaaa cgagaaggtc ggtccatctt ttcttttaat tcttgaacga
      541 tggtatcaac gatcagatat ttgatcagta acccacctaa tgccaatgca agtaaaatca
      601 aactagcaga acctaacata ctcatgatcc cgcctcgaga aagcagttca tctagctttg
      661 gatcaggtgt atttgcgaca taaccattca tcaagatgtc tgctgctttt ccgaccgtca
      721 aacttttctc atggataaaa gaaagaacga gagcagctgt cgatcccgct agtaatgtag
      781 ggatagccgg aatttttttg attgcccccc caaataaaat aactagtgga atcaatgtcc
      841 aaggagagat ccaaaagctc gctttcatgt tttggatcat ttcattgata ctagctagat
      901 cggctgaatg actgttatgt cctaatatcg tataaaaaat caacgaaagg acgaatgctg
      961 ggaaatccgt ataacgcata ccagcgatat gttcgaatag attaactcct ccaatacccg
     1021 ctgccaagtt ggtcgtatct gataaaggcg agcagttatt ccctaaaaag gcaccggaaa
     1081 cgactgcgcc tgtagtcatt gcatcattga atccaagtaa atgaccgatc cccaaaaaag
     1141 caattccaat cgtcgaaatc gttgtaaatg aactgccgac agtcactccg acaattgcac
     1201 aaacaagaaa tgctgtcggt aaaaagaaac gcacagaaac aagtttaaat ccataaacca
     1261 taatcgtagg gatggttcca gataaaatcc atgaagcaac taagacacca atcaaaagaa
     1321 aaatcaggat tggaatgatc cctggagtga ttcctttgat gatcccgtca tggatctcat
     1381 cccatgtgaa ctttttaaat ctgccataaa acaacaaaca catgaaagct gcaaggatcg
     1441 ggatatgtgg cgagagctgg aatccgatga tcagtactcc taggagtgcc aacaacacga
     1501 tcaatacagc gatactttct tttaaagaaa tatttttatt catcgtcgat ctcctatttc
     1561 aaatgaaaag gccctgccat gaaaaaacta ttcacttggc aggacctcct actaaatcag
     1621 cgacaaaacg tcggctgaaa gacttatttt tttatttttc ttctaaactt ttttctacct
     1681 caatccccca agaatctttc ttgaagtgat tgatgatcaa tggaataacg aacatgacca
     1741 ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc tgtacctaat gtagcaaata
     1801 cgacaacgat gatagagaag atcagcatgc agctgactac ccaagctaat ccgtttccgt
     1861 ttttaccaat tcggtaagga cgttcgaatt ctggatgttt cttacgcatt ttaagcaatg
     1921 aaacagcaat caaaatgtac acgatacaat aaataacagt tgtagcattt gtaagtgtca
     1981 agaatacagc acttacgtca tccattacac cataaagcaa agcaaataat gaaataacga
     2041 ttgattgtgt tagaacgaca tttctagata cgccgtattt gttttcttta tggaatccga
     2101 attttggtgg aaggaatcct tcacgagcta cttggatgat tgtttttgat ggtccagtaa
     2161 cccaagctga taattgtaaa agcacaccaa taaataccat gaagctgaag atatttccga
     2221 tcacttctgg taagcctagg atttgacagt caatcaagat aggctgcgtg atatttgcta
     2281 attccatttt tccatctggt acgacatttg ctacaagcat cgcattgatg acattcaata
     2341 aaactaatcc gatcaaagcg ataaatacgc cttttgtata attttttgtt gcgtctttca
     2401 aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa aataaatgtg ataccagcta
     2461 agtatttcaa tgaatcaata tgttccaagt cagggaaagc ttttgaccat gaaaagtcac
     2521 ctagatagcc gtttgagact agtccgactt tgaaggctgc caaaacacct aaaacgaaca
     2581 tgatgactac tgggatatat acccctagcc atacaccgat gttcccgccg acttttgcca
     2641 tatcgaattt caagttcaag atcgtgatga tccagtagat aactaaaata caacctaaaa
     2701 cgaaccagtg attattgcca agttctacat taccaaatgt gtttcctaac aatggtccta
     2761 aagtagaagc gaccattacc atacctggga acatttgcac ccataatagc catgcaacga
     2821 caaatcccca tttgctgccc agtgctgttt ttacccataa ctgaggaccg ccttcttgag
     2881 gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg accggcaaaa aacaaaactg
     2941 caaaaattga ataaaaaatc aatgtccaac cgactgcggc taacgtagga atactccgaa
     3001 ccgttgcaca cagtgccatt gtcagaccga taaaggtccc aagacccatt ttacttactt
     3061 cagatttact caccgttgac actctccttg attttttgga taaaataacg gtgtctttga
     3121 agtagtttca gcaataaacg aaacaattca gcgaatattt gaggggattc tttgagttta
     3181 ttgcttgaag ggtttcttca aagacaacct caaaacaaag ggatcgaaga gcttaggtct
     3241 ccccttgctc ttcagatcca aacatgcgtc agaaacagtt agtattcaag ctgttttcta
     3301 acatgattac cttgcttatt ttgcttcgct tgccaataat tggtctgcgt agattttttc
     3361 caatttttct tgaagagctg ctttgatttt ttcagcatat tcttcaaagt gttcttcttt
     3421 gttcatgtat ggtgtcatta cgctggcacg caatacagtt acttttccgg cacggttcca
     3481 ttcttcatct gagaatccta gttggttcac aaattgtaat gggctgttgc catagtctgg
     3541 gatagcaaag tcagtatgtg aagttaagaa ctcattaccg taaatgcttc ctttcacgta
     3601 tgaagaataa tcatatacgt catggttcaa tttattcatt gcaactaagt catcgttgcc
     3661 tttttctttg aatacatagt ctaccatatt gaaatctgga taagttaatg gatgaacttc
     3721 gatttctttg tctcctactt taaatgacaa gtcattcaag aagttgtaga aacgatgtgc
     3781 accttcgatt gatgcaccca ttaatttacc atatcctgtg acattcaaag gtaagacatg
     3841 gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt gaaccttcca agatataagc
     3901 cccaagtaat gctgggatat ctgcgccttt ttcaaataca tatgtcgcaa agtaagagat
     3961 aacgtctctc atgcggatat ctttgatgac gataccaccg gcagaatatg gaacatagcc
     4021 cattttatgt gggtcaatcg ttacagattc tgcttcttcg attgctttat acgcactgtg
     4081 tacttcttct aagatataat ctttgttttc tgtaaagaca ttgtatttgt aatgaacatc
     4141 ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct aagaaaatcg cacggccgta
     4201 tccgccataa gcagcatcta cgtgtaagta gaagtaaata ccgtcttttt ctaatacgcg
     4261 acgcaatgca acgattttat cgataccgtc gattgctcct tcttctgttg atcctacaac
     4321 accgactaca cctaggattg gtgttttttc agctgctaga ccacgaacga ttttttcaag
     4381 ttcgttgatg tccatgcgat agttgtggtc aactggcact ggaatcactt ggtctaaacc
     4441 gataccgatg atgtcggcag cttttagcca tgaatagtgt ttagtttgtg gaaccaacca
     4501 ttttcctaat ttttctaaat gttttccgct acgtgctgag tgagctttga tttcgtcgat
     4561 tttttctggg acgctgtcta acagattcat gatttcttct gttgacaagt tcattaattc
     4621 ccaatcgctt tttccagcaa ctagttcagg tgtcacttct ttcattgcaa gcggcaatga
     4681 tttgatgttg cgggcatacc aaagtccttc taagtttgct aaagaaccat cagcaacgat
     4741 gtggccccag ccatctttat agctcattaa ttttgcaaat tccattccta cttcttcttc
     4801 catttggctt gttgcaggag aagattcata ggcaacgtta ttaccattcc atagcatagc
     4861 aaaattgtac gctagtaaag atggcatcaa tgtttcagag ttcatatgac cccagtaacg
     4921 tccggcatta tgccaaggaa cggaatgggt acgcatccgc gctgaaattt ctgatagaac
     4981 atcctttgtt ttattcacag tgtgttcaaa actagcactg cttttttctt ctggcgtaat
     5041 aataggcatg tcttgaggca tgtagttttg acgccagcct aaatgttcat ccaccaattc
     5101 attcaacaaa gctttataga tttggccgtt ctcagctttg tccccaatga atagggcatt
     5161 taggtttaga tctttcgaca atgattcact catagtgaaa ccctcctaaa aaaatattta
     5221 aattttgtcg agccctacat tacacctgtt cttctattct gtaaactaag ttttggcctg
     5281 atttttaatc atattttaat atgtatcaat tatccgaatt gttcacactt ttgaaataat
     5341 taaaaaaaac gagggaattt acaaatgtaa actccctcgc aaagattgta ttaacgacaa
     5401 ccagcgttct attttaggat gggtttcaaa attggaatgt tgtaatagta tttccctttt
     5461 tcaaggtaaa aacttactaa attctaatca attactttgg ccaaaaagta atttttctta
     5521 ccttttcgaa taaccacaaa cttaccatcg aattcatcgg atggatttat gacaaaattt
     5581 aaatcggtaa cgcggtcacc gttaatactt atagctccgt ttgaaacatc ttcacgtgct
     5641 tgacgtttag atggttcaat ttttgtggaa acaagcagtt ccacgatatt ttcaggtgtg
     5701 cttgaaattt caacgttcgg cattttaccg aatccttgcg cgatttcttc cgcatttagt
     5761 tctttaatat ttccagagaa caatgcttgc gttatttttt gcgcttcttt caagtcttct
     5821 tcactatgca caaatcttgt aacttcttct gccaaacggc gttgtgcttc acgtttctca
     5881 ggttctgtct ctactttttt cgctaagtct tcgatttctt cttgagacaa gaaagtaaag
     5941 aatttcaagt atttcactac atcgcgatca tcttgattca accagaattg gtagaactca
     6001 aatggtgaag ttttcttcgg atcaagccaa acggcaccgc ctgcagtttt tccgaatttc
     6061 gttccgtcag ccttcagcat caatgggatc gttaaaccga acgcttctgc ttctggtcct
     6121 tctaattttc ggattaggtc taatccagct gtgatatttc cccattggtc tgctccaccg
     6181 atttgcaatt gcacatcgta tgttttatgc agatgcaaaa agtcgataga ttgcaggatt
     6241 tggtaagtaa actctgtaaa tgagattcct acttccaaac ggctggcaac gatatctttt
     6301 gctaacatcg tgttgatgtt gaagttttta ccgtagtctc tcaaaaattc aagtaaagag
     6361 atttttgaca accagtcgta gttgttcaca aatgtgatat ttgcatcttt accaaacaat
     6421 tttctcattt gattggaaag tgcgtccacg ttatgttgca cttgttccat cgtttgcaac
     6481 acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc ctgttccgcc gccaatcaga
     6541 atatatggat gatggcctgc caattggaat cttttcatca tcataaatgg aatcaaatgt
     6601 ccgatatgca tcgaatcacc agttggatca acgccgcaat aaagtgaaat gctcttttct
     6661 tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat taatggcgtc gcgccaagtt
     6721 aattcatcaa taatattcat tttactgacc tccgtgtagt ttttttatga aagcaggaac
     6781 ctgctagcat gtgtaattaa ttatttcttc aggataggat aggaaataaa aaaagtccct
     6841 tggcatataa gccaagggac gaaatctcgc ggtaccaccc aatttgtatg tctagatatc
     6901 caacatacct ctcgaacatt taacgctgtc gcgtggcaat cactgccaaa gactcctgaa
     6961 atgtaattcg ctttaaagca tgtactggtt tgcagcgacc accagctttc tgaaacattg
     7021 gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt attaatatgt gtctatcata
     7081 gagcgcaact ttttctttgt caacagaaaa aggatatttt ttttaaattc ctcatttttt
     7141 cttttttgaa tccgctctaa ttccgaacat tttattttca attattgtta aaagaaataa
     7201 ctttttctgt attttttctg caatttatcg agaagtcact ttactttgcc tcttattctt
     7261 tcttcgttac aataaggaat gggacaagta aaaaaatgaa aaagcaaggg acgaggtata
     7321 tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc tttaactatc gttattttaa
     7381 ttctaagttt gttttttgtc atccgatcat ctacaaaaaa agaaccaatc attcctgaaa
     7441 aaaaaataac agaaaaagca aaagaaccga cagatgataa gcaacaacta gttactcaag
     7501 caaaaaatct ggctcaaagc tacgattatg accaagcgat tgcgttattg gaaaaagata
     7561 gtaagaacgg atctactgaa acacaacagc ttcttcaaac actaaaaaag gagaaagaac
     7621 aactgatcga ctggtctgat cctactcaga tctctcatgt ctttttccac agcttgatcg
     7681 tggacccgca aaaagccttc cactctcaac aggcacaagg atacaaagat tatatggtga
     7741 ctgttgaaga attcaaccgt tcgatcgaac aactttatca aaatgatttt gttttagtta
     7801 atttgaatga tttgatccaa aaagacgaac aaggaaatct ttcctttaac gggcttgcgc
     7861 tacctgaagg aaaaaaacca ttggttcttt cacaagatga tgtcagctat tatgaatata
     7921 tggataattc cggttttcct agccgattgg tcgtatcaaa taacaatgaa atcaaaaatg
     7981 tctataaaga gaaagataaa gaagaaatcg gtgactttga catggttcct ctgatcgatg
     8041 cctttgtcaa gaaacatcct gatttttctt atcgaggcgc aaaaggtacc ttggccctga
     8101 caggttacaa cggagtttta ggttatcgaa catcaaaatc tcaatatgga gacaatgaaa
     8161 aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga acagttgaag aaagacggct
     8221 ggagctttgc ttcccataca tggggtcact tgaatatgac tcaagcttca ctagaagatg
     8281 taaaaaaaga caatgaattg tggcagcaag aagttgcacc tatcctagga aaaacaaata
     8341 ttctgattta tccatttggt gcagacatca gcgactggca gccttattca gcagaaaatc
     8401 aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc gatgcttcta
     8461 caccagcttg gggacaatta ggtacggatt tctatcgaaa tgctcggatc aatatcgatg
     8521 gcattcgttt tcaatctgat ttagacggaa aaaatcctat tcttgatcca ttcatcaatg
     8581 tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg attttacgtc atttatgaac
     8641 caaaaaagcc gaactgacat tttatcgtca gtttcggctt ttttatgctt tatttcttct
     8701 tcggtgcgat tcccttgatc aggacttctg tccattctgc tgtatatgga agcagttttg
     8761 cgtatatctc cggcttagaa attccttcta ccattgcttg aaaatagagg atcaagaact
     8821 catccgaata tttcaaatcc acttttcctt ctttccgccc tcgatggaaa agatcaagca
     8881 tgatactgaa actttcttgc gtatacgcat ccatcgcgtc agaaaattct tttttttctt
     8941 tttttgtata gtatgtcatc aaatcgatat aaaattgttt gttcactcga ttgagataac
     9001 ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa tggaagattt tccgcaatca
     9061 acgctttcgc ttcttgccct aaatcattca taaatgcaaa aaatacttca taaatcagcc
     9121 cttccttgct gccaaaatat ttaaaaatcg tcgctttact tacctgagcg ttctcagcta
     9181 ccttatccat tgtcactcca gcggttccta tgtctgtgtt catcaagtca aaagctgctt
     9241 gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa accgttcatt tgtagagcct
     9301 cccgtaattt taaactttta taatatatat agttcaaatt tattctgagg tttattataa
     9361 ctatctattg agaaagtatc aagctagcaa ttccttgttt atgaattcat tgacagaaat
     9421 ttataaaaag aatattatgt actaaaataa taaaaaaagt tttataaaaa ggtataaaac
     9481 ctttttatta aactatttaa tcttgtttag tatatatttt tagaaaaagg agtggggttt
     9541 ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag ctttggtcgt ttcgaagctt
     9601 taaaagatgt ctctttcacc gtacattcag gtgaagtcgt tggatttatc ggaccaaatg
     9661 gttctggaaa atccacgacg attcggacac ttttagggat tataaaaaaa gacggcggaa
     9721 cggcaacgat ttttgggcaa gatgtatggg aagatagttt atctatccat gaacgtcttt
     9781 cttatgttcc aggcgatatc tctttgtggg gaaatttgac tggaggagcg atcatcgatc
     9841 tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa agatgaactg attcaacgtt
     9901 tctctcttga tcctaagaaa aaagcaaaaa actattcaaa aggaaatcgc caaaaagtgg
     9961 gcttgattgc tgctttggct gttgattcgg atctctattt atttgatgag cctacttctg
    10021 gtttagatcc tttgatggaa acgattttcc aagaagaagt tgaaaaattg aaagaacagg
    10081 gcaaatccat cctactttct tctcatattc tcagtgaagt cgagcgacta gcggataaag
    10141 tcgtgatcat acgcgaagga acactcgatg aagaaggaac actcgatgaa ttgcgtcatt
    10201 tgactcgttc gaccatcact atagaaacag aagcagagaa tagtcactta gcacaattga
    10261 acggtgtcca tgactttgtg caaaaaggtc acctcgctca atttgctgct gataacgaag
    10321 ctttcgatgt gattttagct gaagcgtcca aattaggtgt gaaaaaattt gaatctattc
    10381 caccgactct tgaggattta ttcatgagac attatgaagg atgaggagga agcgaaatga
    10441 ctgataaatt ttcacgctgg tccacccttt tttctcaaaa ccttagccgt gactggaaaa
    10501 agatcctgat atggctagtc ggtgtcactg ctttttctgg tggttttgtc cctgcgtttg
    10561 aagaattgac ttccggacaa ggattagctg gattatacga aaccttgcaa aacccggcaa
    10621 tgattgcaat ggtcggacca actccagctt cgaatgctgc tgagtatact ttaggagcga
    10681 tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc tgctgtgatc tctgttttac
    10741 acgtggtgag ccaaacgaga aaaaaagaag aacttggtct gattgaactt attcgttcct
    10801 ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc tgaaagtatt ctgatcaatt
    10861 tgcttttagc aatatcgatc agctgtacga tgattttttt ccaagcggat tcgatcactg
    10921 cagaaggtgc ttggcttttt gggctatctg taggtagtat ggggattttg gggagtgccg
    10981 ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag ttcgaatggt ctgtctttag
    11041 cgtttttagg tggactttac cttctacgag caataacaga tgtagctgat tcctcccttt
    11101 cgatgtggaa tcctttaggc tggtcttatt tgacctatcc atttacaact aacaactgga
    11161 ttcctttgct ttatttgttt ttgttcagtc taatcttgat aatcagttca tttgttttag
    11221 aggaacatcg agatttgaac accggctata tccccgaacg tgaaggaaaa ccgtatgcta
    11281 aacgttctct tttatccttg cgtggtttat tattctatct aaataaaggg atgacaatca
    11341 gttggcttat cacttttggt ttgatggggg cggcatacgg ctctatctac gggaatatgc
    11401 aacagttttt agaaagtaac gacttaatga aacagatgtt tatccaatct ggcgtctcaa
    11461 ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat ttgtctagct gctattttac
    11521 caattgctag catcaatcgt ttatttacag aagaaaccca cggacgtatg aatcagctgt
    11581 tcgcaacaaa aatttctagg aaccaacttt attggaccaa tatgttacta gctattctat
    11641 ttgctatatt ggcaatcctg attagttctg gtgcactagg gctttctgct ttggcttcga
    11701 tgaaggatgc gaaagacatg gatctattga tgtttcttaa agccgggttc aatttcctgc
    11761 ctgccgtttt agtgtttatc ggaattagtg gctttctatt cggatggctt cctcgaatcg
    11821 gtaaacttgc ttatatctac ttagcttact cctttgcact caattacttt ggcggaattt
    11881 tggatttacc agaatggttt tccaaaacag ctgtccctag ctgggttcca cggatgccaa
    11941 acgattcttt tgatctttcc gtgttcctta cgttatcatg tatcagcctt gcactgttcg
    12001 tccttggttc tattggttat aggcgaagag acttactaga aggataataa aaaagcatgt
    12061 acaatttcat gctagcctcc aaatctgggt caaaaatcca acatttggag gtcatttttt
    12121 tatttacaag tcttctaact gctctttatc aatgatccag acttctcgat tctttctttt
    12181 tacataacca ttctcctcta ataatttaaa ttttctagat aaagtttcgg gggtcgtacc
    12241 gataaatgca gcaagttctt tcattttcat tggcaggaaa aagtgatcct tccctgtcgc
    12301 tttagctaag tcaagcagat agacagcgac tcgttcttct actttttcca tcatcaaaaa
    12361 agttgtctgc tgctgtactt ttgttaattt ctcaacgttg attgctaaaa gtttcagact
    12421 aagggcggga taatgcagta agagttccga gaaatctttt tgtctcaata tgcaaatttc
    12481 cgttttttct agtgcttcgc caaataaaga ctcatttgga acgcctagca actggctctc
    12541 tccctcatat ccgcccggct ctattactcg taaaagctgt tcctttccgc tagcagataa
    12601 ttgatacact ttcatactgc cggaggccac gatcactaat tgaggttctc cattcggcgc
    12661 aagaatctgt tctccttttt ccacatagtg atgtttgaca agtgcgtgta ctttttcttg
    12721 atcttgctgt actaagtgat tgaacaaagg aacaaatgaa acacataaat gatcatgact
    12781 cataatctct cctccttcta aacagctatt tctaataaag aaacataact tcccttattc
    12841 caactgataa ttttctttta tcatagcata aacgtcaagc gaaccagata ccagcaaata
    12901 gagtggattt ctttttgtcc cttgctttat tttccttctt ttgagacctt aagtaattga
    12961 ttcctatcaa ttttttaaat atttactttt tgaataatca aaaacaaacc ttcactcgca
    13021 agccgagagt aaggtttgtt tttcgcattg cttatttcgt gttgtctatt gaaatttttc
    13081 cgcacctagc ttttgatata gtggtgctag ttcttgtaag gtttcagcgg ttatttgctc
    13141 aacgtgttcg atttcttgcg gatagaagaa tagtttaccc atttgaaagg ttcggtctct
    13201 catcgctttt ttcaactctt cttgtttctt tgtatcggac aaatagtcag acaaagctaa
    13261 gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa taatccttga tatcgactgt
    13321 tttgctccac tctttcagta tcgcaagcaa ctggttatac gctgcaatgc taccatatcg
    13381 ttcttcgctt ttataatgtt ggtacatcaa atagatttgg tattgttttt gattcaacaa
    13441 aactgctaga caggcattct cgtcttgacg agattcgctt cggtacgcgc tccaaaaatg
    13501 actgcgtaaa ttccagccat tcgtccagct ttcgatttta ggtttgctca tgccgtacat
    13561 atcaggcaaa agtgcagctg tctggagctg aatctctttc catttttgcc agtgttcttt
    13621 atattctttc ttgatttgtt caatcttatc tgttggatgg ctctctttga gctgtttgaa
    13681 tgcgtagatt ggtcgatcaa acaactgata atctgatttt ccaagttcca ttgtttcgcc
    13741 tctttccgtt atcttttgta ttctacctga attttgcgat atggacaaat agaggttgtg
    13801 aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca aaaaactgcg atgagcatca
    13861 ccgcagtttt attggctcta taattt
//
LOCUS       DAGCWU010000063        13735 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.063, whole genome shotgun sequence.
ACCESSION   DAGCWU010000063 DAGCWU010000000
VERSION     DAGCWU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.063"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            162..803
                     /locus_tag="MBZ71_001475"
     CDS             162..803
                     /locus_tag="MBZ71_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4429436.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            846..1538
                     /locus_tag="MBZ71_001476"
     CDS             846..1538
                     /locus_tag="MBZ71_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBT4429437.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMEQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQDFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            1535..2437
                     /locus_tag="MBZ71_001477"
     CDS             1535..2437
                     /locus_tag="MBZ71_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4429438.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQLG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     LDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2595..4301)
                     /gene="efbA"
                     /locus_tag="MBZ71_001478"
     CDS             complement(2595..4301)
                     /gene="efbA"
                     /locus_tag="MBZ71_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBT4429439.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIAELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFHRLSQTELEPKAIQRQFQGIGFDTAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKNFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRDGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTEYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            4491..5114
                     /locus_tag="MBZ71_001479"
     CDS             4491..5114
                     /locus_tag="MBZ71_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429440.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIRQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            5564..6613
                     /locus_tag="MBZ71_001480"
     CDS             5564..6613
                     /locus_tag="MBZ71_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4429441.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDVLIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVA
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            6610..7491
                     /locus_tag="MBZ71_001481"
     CDS             6610..7491
                     /locus_tag="MBZ71_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4429442.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            7494..8264
                     /locus_tag="MBZ71_001482"
     CDS             7494..8264
                     /locus_tag="MBZ71_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4429443.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCKEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            8543..9397
                     /gene="aroE"
                     /locus_tag="MBZ71_001483"
     CDS             8543..9397
                     /gene="aroE"
                     /locus_tag="MBZ71_001483"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBT4429444.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            9575..10738
                     /locus_tag="MBZ71_001484"
     CDS             9575..10738
                     /locus_tag="MBZ71_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBT4429445.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            10855..11265
                     /locus_tag="MBZ71_001485"
     CDS             10855..11265
                     /locus_tag="MBZ71_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4429446.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            11439..13667
                     /gene="topB"
                     /locus_tag="MBZ71_001486"
     CDS             11439..13667
                     /gene="topB"
                     /locus_tag="MBZ71_001486"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBT4429447.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT         4592 a         2342 c         2955 g         3846 t
ORIGIN      
        1 gaagagaata atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa
       61 gggagctgtt tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat
      121 atgatattcg gaaaaagaaa tctcaaaggg aggtaacttt tatgggattg aatgaattta
      181 ttttaggatt gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag
      241 agcatacagt tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca
      301 ttgaagaagc gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc
      361 atgactacac agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag
      421 agttaagagg gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag
      481 agattccagc tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa
      541 ccgtagaagg agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg
      601 atgaaatcat caaaaataac ccggaaaaag tttataagga gatgtttatt gaggcagtga
      661 ctacgattaa aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc
      721 cggaactatt gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga
      781 ttgtaaagaa agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa
      841 agaatatgga tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt
      901 tatgtgattg gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca
      961 agggggaaga aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt
     1021 ttactgacaa ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg
     1081 tagagttcaa gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac
     1141 atatcatcaa cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa
     1201 aagagaatct agtcgtgggt gtcatgtacc agctaatgga acaggaaggt tggctgtata
     1261 atcaagaaaa tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg
     1321 tgttctttcc ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta
     1381 ccagcgggcc aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagattttc
     1441 ttctcccctt cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg
     1501 gaagagaaat ttattatttc gaggagtttg agcaatgaat atccagcaga tgaaatacgt
     1561 ggtcgctgta gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca
     1621 gccaagtctt tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac
     1681 tcgaacgaat cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga
     1741 gaaaatattg gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga
     1801 caatttttcg atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact
     1861 cgaggaattc aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt
     1921 gatcgaagaa gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa
     1981 tcaagctggt atcagaagat atttacaact aggagagcta agtggccgca gcattggaaa
     2041 attcaaaacg catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac
     2101 caaagaagaa ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc
     2161 gtatttttat gaagacctta tcgagatcct tgatcaagaa acagtgattt atacgagtga
     2221 tcgaggtact ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat
     2281 tgtgattgga gggattcgcg agcatttgcg gttaatccct ttggcagaag gcgaactaaa
     2341 tgaattttat atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat
     2401 caaagagctt actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt
     2461 tattgaacac cgttaatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt
     2521 tttgtagcaa acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta
     2581 gaaacactca ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc
     2641 acgataatcg tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt
     2701 cggatatgct ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa
     2761 aaataggcag ctaactcggc agcttctgta attgtttcat cggagggctt gtcactttta
     2821 atgatgacgt gcgaaccggg tatatttttt gcatgaagcc aatattcggt ttttttagct
     2881 gtcttcattg tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg
     2941 tcagaagaaa agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc
     3001 ttctttttca ggtatccttc tgaagtcaat tcttctttga ttgcttcgat atccattggg
     3061 ccggcaattt ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct
     3121 tggatttgtt cgccaatcag cttaacagcg ttcttcagct tttggtaacg atggaaatat
     3181 ttctgtgcat tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct
     3241 tcttcataat aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa
     3301 gtagtaagta gttcgccatc tctacggtaa ttctcagcgt tttcggattc tttgagtgtt
     3361 tgttcacgtt ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc
     3421 agttctccgc cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct
     3481 aataattgac tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga
     3541 taagcaatcg gtgtgaaaaa atttttattt tccgtttcgt aaaaagtagg aataggctga
     3601 tggctgatgg ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt
     3661 aatcttttgg tcagctcttg agcggtatcg aaaccgattc cctgaaactg acgttggatt
     3721 gcttttggct ctagttcagt ttgggaaagc cgatgaaaga ttttttcttt ctcggagcta
     3781 aatgggttca agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa
     3841 cgataagtgt tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct
     3901 cgattaacta agacgatcgt actatgacgt cccattaatt cagcgatcaa tatgatattt
     3961 tgtagatcac ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg
     4021 atttgttcga tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag
     4081 ttaggcggat tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt
     4141 gggtgcgcag ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt
     4201 tcattttcat aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat
     4261 tcgttgacca tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt
     4321 ttcataagta tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa
     4381 agtaaaagtc tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa
     4441 atccaaattt ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta
     4501 tttgggaagt agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg
     4561 cagaaaatcc aagtaagtat tttcgacgtc ttgtgtttct gctcatctta tcattgctgt
     4621 taacagttgc aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg
     4681 tttattcgtc cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga
     4741 aaatcagaca aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc
     4801 aggacatgct tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct
     4861 tttattatcc gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg
     4921 atgaagtgac atttattttt tttgatggag gaaaagaagc aaatcggata actgcgggta
     4981 agggaggaat aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag
     5041 ccaatatcaa acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg
     5101 agattgctaa ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa
     5161 ttatcagaac aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt
     5221 tgacgaaatt caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta
     5281 atgaacttat caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga
     5341 taagttgtat taatggtgag actttcccat agatacaact ttttagagtg catttctaaa
     5401 gcttgtatct atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt
     5461 tttgtgtaaa gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc
     5521 taattataat aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt
     5581 aataacagtt gtagtaatta ttttagttta tttagcaggc gcatttattt atggaaaagc
     5641 ctcaggcgaa aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt
     5701 attgcaatac gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct
     5761 gaaagaagaa ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc
     5821 cgatcagagc aagctagcca caatgagtca acagctagta caatctgatc cagatgtctt
     5881 gattggaatc gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt
     5941 tgtgttaggt gctgtaacgg atcctgtggg ggctggatta gttgcttctt tagagaaacc
     6001 tggtggtaat gtaacaggtg tttctgatca gccgcctgtt gcttcacaaa tcaagcttgc
     6061 ttctgatttg ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa
     6121 ttcaaaatac caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt
     6181 aaaatatgcg gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca
     6241 agtcgatttt atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt
     6301 aaaagaagca aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca
     6361 aggtggacta gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat
     6421 ggcagcagaa cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga
     6481 tcaaggggat acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca
     6541 atcgatgaag gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga
     6601 ggatgacaaa tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta
     6661 gggattttca tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc
     6721 ccattaggtg ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct
     6781 acattattag gagtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca
     6841 aaaggaaaga taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt
     6901 attttgtttg tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac
     6961 agcttccatc gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct
     7021 ttgggcatct tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat
     7081 attgcgacag gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg
     7141 aaaattttag gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc
     7201 cagaacgacg gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct
     7261 tccatcatca tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct
     7321 attgttgtcg gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt
     7381 gacacgactt atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg
     7441 caattgaaga aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg
     7501 atatattgac aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag
     7561 tactattgga tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg
     7621 ggaatggtgc tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc
     7681 aaggatcgat ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac
     7741 gattcctttc tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag
     7801 cagaaaattt gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact
     7861 tgaaaaatca aaaagaagat tttttcagac tatgtaaaga agtcggtaat ggcctagagc
     7921 aacacttgga tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc
     7981 tgatggcgac gatcacagct cctgagttac tattattaga tgaacatact gcagctttag
     8041 atcctaaaac agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat
     8101 tgacttgtct catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga
     8161 tcgtactcca aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga
     8221 caatgggcga ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt
     8281 cctttcagta gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta
     8341 aaacaataaa caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta
     8401 acaattaaat agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact
     8461 ttgccaccta gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa
     8521 gtaagaacaa aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc
     8581 tgatccggct tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa
     8641 catcgatgcg gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga
     8701 aagtattcga acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt
     8761 tattccttat ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat
     8821 cgtgcacgaa aagggtcgtt tgattggcta taataccgat ggtgcgggct tcatgcaatc
     8881 cgtgcaagaa gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg
     8941 tgcagcaaag gcagtcactg ttcaagctgc gttagacggc gctgaggaaa tcgtagttta
     9001 taaacgaaac aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg
     9061 aacttcttgt aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt
     9121 gaaagaaagc tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca
     9181 gtcaccgctc ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta
     9241 tgcacctaga gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa
     9301 tggacttggc atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa
     9361 gatgccgatc catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct
     9421 tttattctcg cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt
     9481 cgttattttt tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc
     9541 tttatttatc agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga
     9601 catccataag aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga
     9661 acgaaaacga acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca
     9721 gcattcattt gaacatttga accaatacga gcaacagata tacagagatg caccaagagc
     9781 agaagaacaa caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa
     9841 acctcgaaga aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct
     9901 ttattcaggt atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga
     9961 ggcagcgact tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt
    10021 gatccttgga agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt
    10081 acagttggat ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt
    10141 tacgatccca ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga
    10201 tctagtgcgt caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt
    10261 cgatttcaaa tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga
    10321 tgcagaaaaa gatctgaatc tggatggtgt agatattaaa aaaggggtcc agaagatgga
    10381 tggacatgtt cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg
    10441 tgtcagacgc caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa
    10501 tctgatccgt gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac
    10561 tgcttggttc ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg
    10621 aataagtgtg ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat
    10681 tcttgaaatt gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc
    10741 ataaaaaaga tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca
    10801 ctcgtaaaaa acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat
    10861 atctatctga acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt
    10921 cagactgaag catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac
    10981 ttgccagaag aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta
    11041 gttatgtttg cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt
    11101 tctttagtca tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct
    11161 gagggtggta agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg
    11221 gtaactgatc gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat
    11281 attttctgga aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt
    11341 ctatttcgac tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata
    11401 gagaagtccg caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg
    11461 aaaaaccttc acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag
    11521 ggtattatga aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg
    11581 gacatttggt agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt
    11641 tagaaaattt gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg
    11701 cacaattttc gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag
    11761 atggtgatcg agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt
    11821 ctaaggaaaa gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg
    11881 aaggttttaa gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag
    11941 caaggcagaa cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac
    12001 ttcaacgaaa agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct
    12061 atatgattta tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag
    12121 gagaaggttt aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag
    12181 catttaaaac ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta
    12241 agcaaaacgg acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt
    12301 tattttcgct atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag
    12361 ctacacttga tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg
    12421 attcctttta cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata
    12481 aaaaattttt aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg
    12541 tcgatgcgaa aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc
    12601 ctgcacgctt tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca
    12661 ctactgtggc tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg
    12721 tacagcaatt aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa
    12781 tcttacgcat caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg
    12841 aagaagcaga agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata
    12901 ccgaaggaac gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag
    12961 cgcaagaaat tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca
    13021 ttgaaaatct taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc
    13081 caaaaggaat cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa
    13141 tgactgcgcg ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg
    13201 tttttttaga gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa
    13261 tcgacaaggt agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga
    13321 agaaaaacga tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc
    13381 ctaaaatcgc tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta
    13441 aaaagaagct gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag
    13501 ttgtcaaagg ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag
    13561 atatgacaat cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg
    13621 gaaacaaaga aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga
    13681 aatagcggaa tatttattcc gaaaagtgag aatataaata ccaataaaac taaaa
//
LOCUS       DAGCWU010000064        13716 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.064, whole genome shotgun sequence.
ACCESSION   DAGCWU010000064 DAGCWU010000000
VERSION     DAGCWU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.064"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            261..599
                     /locus_tag="MBZ71_001487"
     CDS             261..599
                     /locus_tag="MBZ71_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429448.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(804..1043)
                     /locus_tag="MBZ71_001488"
     CDS             complement(804..1043)
                     /locus_tag="MBZ71_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBT4429449.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1019..1366)
                     /locus_tag="MBZ71_001489"
     CDS             complement(1019..1366)
                     /locus_tag="MBZ71_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBT4429450.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2098..2679)
                     /locus_tag="MBZ71_001490"
     CDS             complement(2098..2679)
                     /locus_tag="MBZ71_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4429451.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2862..3488)
                     /locus_tag="MBZ71_001491"
     CDS             complement(2862..3488)
                     /locus_tag="MBZ71_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4429452.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3510..4841)
                     /locus_tag="MBZ71_001492"
     CDS             complement(3510..4841)
                     /locus_tag="MBZ71_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBT4429453.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4996..5397)
                     /locus_tag="MBZ71_001493"
     CDS             complement(4996..5397)
                     /locus_tag="MBZ71_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBT4429454.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5483..5689)
                     /locus_tag="MBZ71_001494"
     CDS             complement(5483..5689)
                     /locus_tag="MBZ71_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429455.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5784..7286)
                     /locus_tag="MBZ71_001495"
     CDS             complement(5784..7286)
                     /locus_tag="MBZ71_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBT4429456.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7304..7759)
                     /gene="argR"
                     /locus_tag="MBZ71_001496"
     CDS             complement(7304..7759)
                     /gene="argR"
                     /locus_tag="MBZ71_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBT4429457.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7857..8474)
                     /locus_tag="MBZ71_001497"
     CDS             complement(7857..8474)
                     /locus_tag="MBZ71_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBT4429458.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8695..10068
                     /locus_tag="MBZ71_001498"
     CDS             8695..10068
                     /locus_tag="MBZ71_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4429459.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10143..11255
                     /locus_tag="MBZ71_001499"
     CDS             10143..11255
                     /locus_tag="MBZ71_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBT4429460.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11327..12964)
                     /locus_tag="MBZ71_001500"
     CDS             complement(11327..12964)
                     /locus_tag="MBZ71_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBT4429461.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13132..>13716)
                     /locus_tag="MBZ71_001501"
     CDS             complement(13132..>13716)
                     /locus_tag="MBZ71_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4429462.1"
                     /translation="LGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKISQKFAGDN
                     SMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTETATQEHTYV
                     APVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQLDASYLNGD
                     YSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3708 a         2884 c         2458 g         4666 t
ORIGIN      
        1 actatataac actaagtaac gcaaaattcc cctttgtaaa ctatctagaa aattgttttg
       61 tttaaagtat atcttattgg atataaatag aaataaattg tagccagttc attttttctt
      121 taactaaaaa aataatccaa gaaacattga acctatggat ccaaaagcta cccaaatact
      181 atagctaacc ccccgctacc ttcaattttg cagataaaac gctttgggag aaaatttcaa
      241 agacaacagg ccatcctatc atgcccgatt ataaacaaaa agctggctat tccttttgct
      301 ctgacaaatt tcaattggga aagttcacga aactagcagg tgaagaagtt tcaactgttc
      361 ggatcaaaga atgcccgctt caaatagaaa cattggttca acgaatttca actagacaag
      421 actttgctat tgtagaacgt gagatcgtag ctatttttgt agaagaaggg attttacatg
      481 atccaactca tatcaatgtg gataaatggc aacctcttat ctataaattc agagaatata
      541 caactgccaa ccagtcgtta actttgaatt ttagattcca agaattcaaa gagtcataat
      601 gataaacatc gttaggtata aataaaaaag actcgcagca cgagtaaaaa acaaatagaa
      661 gctatacatt agaaaggtcg actgtgaccc tatagatgca gtatatgcag cttatgagca
      721 tttcttctgt tctctttgtt tttgggatct gtgctgaagt ctttttcgtt ttttgccgct
      781 ctttctttga aaaatcgatt atgttatacc ttctccatta ctaattcagg catctgctga
      841 gtaatgattt ttcgcgcaaa agaaatatct gttacccaag gttcgatttg ttcttcagtc
      901 aagacaagcg gcattcggtc atggattggt gcgactgtgc ggttaggttc agtagtcatt
      961 atgattgatt ctgccatttt ttcatcctga tggctacgat aaaaaccgcc aatatacact
     1021 attttttcct tatttgaaaa cataaatttg tctttttctt cattccactc attgggaaaa
     1081 cacaacgatt ttgcaaaaat ggttgatgaa aggtcttttt ttcttctact gtctctgcac
     1141 gggcattaat catcaattgt ccttttttaa atccggtaaa tccccacttc atgatttccg
     1201 gagtgaggat gttttcttcc tcttttctta acgtcacaac aagatttgac ggaaaaactt
     1261 ctccagctgc taattctgct gcctgcttcg gttgtttcgt ctgggcttct cgataataat
     1321 tctgaaggag ctctgactct agatcaaaaa aataacgacc acacatccta atccctccca
     1381 atcttttttt ctatcataca ctgcaacaag aagttcgcac caacaaaacg ctttcaattt
     1441 ttgtagaact aaaaaagttg tgacaagtga caaactctcc agtgaatggt attctcctgc
     1501 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgctt tttgaaattg
     1561 ttccttgtga ctcggagtga aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa
     1621 gccagtttct cggtattaag ccaaatttat gcaaaaatgt agaaagctat tttcacaaaa
     1681 tttttcttaa tactcgaaac taagcgactt tttcacaacc tcttccttaa acggtgttca
     1741 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagcag ctatttttat
     1801 ttggctgttc ttattacttg atgcagggca gctttttcac aacctctcca ctaaacggtg
     1861 ttcaatcagc taaccttcgg tattagctca aaaaacggca aaatatgagg aactattttg
     1921 ccgtttgttt ctctaatact caggtcaaag cgctgatttc acaacctcta atacacggtg
     1981 ttccagaagt agcttcttcg aaaaataagc cgaaatttca caaaaatttg agaagcaatt
     2041 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct ctaattatta
     2101 aagaaccttt tgcgaagtta acaatctttc tttgatcaat tgaaaattct gcctaagtag
     2161 tgatagatcc tgtgtgactt tcaacaataa gatacttccc tggatttcag caatcaacgc
     2221 tttggcttga cggtctgctt cttcatcgga cagtttttct tcagaaagtg ccaggatatt
     2281 ttgtttcgta tacttgaccc attgattgac taattctagt agcaacagac ggtattcttc
     2341 atccactgta ctcaactcaa taatcaaatt acctacagga cagccatgat aagtgatctg
     2401 tgtctcatga tcagcgaaaa tccagtccaa catttcttct aatgcttctt ctggctgttt
     2461 tccagcattt aagatgtctt cgaacaattc tgtctgccat acgtccatat gcgcatggat
     2521 caccgctaga caaacttctt tttttgaaga aaagtaataa taaaattgac cttttcctgt
     2581 atcagaagct gctagaatat ctcggattga cgtttcatga tagccttttt tataaataag
     2641 atctttggtc gcttccaaga tcttttcctt tgttttcaat gatattctcc tttttcattt
     2701 ctcatagtcc tatattatac agtaaattca tcattttttc ttcttaatcg tttaaaaaag
     2761 actgtgccaa attatctgac acagccctca ctataaaact gttctcatat agctagatgt
     2821 tcattgattc aagaactgcg aggacaaaag caattcctat cttatgatag ttgctctaat
     2881 acttcctctg ttgtcatcac ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc
     2941 attgcttctt ggccaggagc accaattgca tctgtcgcta ccacaacatt gaatccactc
     3001 tctaccaatt cacgcatatg ggaatcgcta cacaagtttc cgttcattcc accaagaatt
     3061 actgtgtcga ttccattttt tctcaattgg agcgccaaat cattgctttc tggtccaaag
     3121 attttatgag gagagctgat gatcgtattt tcatccaaat aaggtttcaa ttcttcgata
     3181 aaatcggctc ctgaaccttc ttccactgat tcatattgac tttttctagc aaacatctgc
     3241 tgctcaagca tcatatgttc tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa
     3301 aaataatgag gagaaataaa gagtttgtat cctttttctt ttgcttgttt taccatttca
     3361 atcatatttt cgatcgtatt gtatttttct aagatatctt ttgtcaaacc aaagccaccg
     3421 cctgctggtt tcaagaactc gacttgcggg tcagtgagaa ctactgctgt atttttatta
     3481 ttgaacatgg tggattctcc attcgttatt tattttaata catcatttag agcttcactc
     3541 attgtcggat gagaatagat ttgatctctc aataacgtat aaggaagttt tccattcata
     3601 gctagcgaaa tcaagttgat gacttcatga gaatcttccg catagatact tgcccctaga
     3661 atcaagtcag tttcagggtc gactaaaact ttgaatacac ctttaggatc ttctaatact
     3721 tgtgctttag ggacaccgct tgtcatatac ttgaataatt tgtaattgac gccttgtgct
     3781 ttggcttgtt tttctgttaa acctacattg gataaagtcg gagtgataaa tacactgtaa
     3841 ggcacagctt tccggttatt cgttgttcgt ttattctcgc ccttcaactg atccataatg
     3901 attcgataat catctagaga gatataagtg aactgcaatc cgcctttgac gtctccaatc
     3961 gcccaaatgt tgtctgcatc tgtacgaagg tattcatcca cagcgattgc tccgcgatct
     4021 gttagtttaa catctgtatt ttctaatcca agtgatgctg tacttggttt tcttccagtt
     4081 gcagccaaaa cttttgacgc ttggattgtt acttcttggc catcttctgt aaaggtgacg
     4141 gcagctgttg attcttggtc tgctacttga tctacagata cacctagatg gaattcgatt
     4201 cccgcatttg tcatgtcttc taaaatcatt tgcgcgatat cttcatcttc acgtggaaga
     4261 aattcagaat gggcatcaag aacgacaact ttagatccgt attcattgaa cattgatgca
     4321 aattccaagc caatatatcc agccccgatg atcactaatt tttccggtaa ttgttcctga
     4381 tccatcgctt gcgtagaatc aatcaagtag cgacttgtat tcaatccttt gattggtaaa
     4441 atgattggtt cagaaccagt attgatgaaa atccgttcgc cttcaacaat cactttttca
     4501 ccattattcg tcacttcgat ttgatggtta gaaagaaaac gtgctgtacc gtccatcact
     4561 gtcccagttg cctcatcgct gatcatatga taatttttgg cgcgaagcat gcccgtcaat
     4621 ttttctttcc gagcaacagc ctctgtaaag tcgattcctc tttctccatt gaaaatcaat
     4681 gatttagaag gaatacagcc gatgttgata catgttccgc catacatacg tgttgacttt
     4741 tcaacgacta aaaccgattc accttttgaa gctaacgcct tcgctaaagt cttaccgccc
     4801 tttccaaaac cgatcacaat attttgatat ttttccatca tttttccctc gcttcttttt
     4861 tacctaccga ttggtacaaa aatatgataa ccgataattg tctattctcc aaatcatctg
     4921 ctttatttgt caaaaaaaac cagacaaaaa gcggtccttt cacgttttta gtctggttcg
     4981 atttccttta ttcttctaaa caatcgttac ttctacatcg atatttcctc tagttgcttt
     5041 ggagtaaggg cagaattcat gcgcatcttc tgcaattttt tgggcttctt cttcgctgat
     5101 gccatcgata tgcacttcta atactacacc gatctgatag ccgccatcat ctaaattata
     5161 aagagagaca cgtgctttta ctgtggactc tgctgtcatt cctgcttctt tgatcatatg
     5221 ttgtaccgcc ccattgaaac aagaagcata acctgctgca aataattgtt ctggatttgt
     5281 tccttctgca tggattcctg gaggagtgat tttcatatgc atcgagccat ttggtgcctc
     5341 gacttctcca ttgcgcccgc catggttgat cattgctgtt tcgtatgctt ttttcattgt
     5401 actgctcctt tcgattgacc atttgctact tggctataat tatagtgtat gacaagtaag
     5461 gcgctttcta caaaaattct gcttattcga ataccagaat gccgtcttct actatttttc
     5521 caaaaaacaa aaaaatacta ctcgtgaaca acagcgtcat atttgttcca aaataaatag
     5581 caatcttttc tacataatct tttatatgat tgattttaag atacatatat accagaaagt
     5641 tgaccccata aaccaatact gttaaaaaaa taaccatatt cgttaccatt ttttcctcct
     5701 tttgctggtc tccctaccag cactcgattt aaccatacca ctttttttgc cgtccaagca
     5761 actagccttt caaaaagact ggtttattct gctgttaatg attcaagatc agaaaaatgt
     5821 ccgaataatt ttgctaattt tactaacgcc aacttgttct ttccgaattc atcattttct
     5881 atctgtaata ctagtaacgg ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg
     5941 tatggctctt ctagatcata tcgaatacct tcttcatttt ccgggtcgaa gttcatccct
     6001 ttttcttctt ccaacagtcg cttcatttct tctaatatct tgtttccttg ttcgcgtggt
     6061 tcatcgctga acaattttaa tcggatttct tgtacttcta cgggctgttt caactctttg
     6121 atttgttcat ctaatgtgat accttttctt tttaattctg gcaaaagcat caggatcttt
     6181 gcgatgacat atgcaccatc atccagaaaa tagttttcct tgaatgcagc gtgtccgctt
     6241 gtttcgatgg ctagctgtgt atctttccca agacggttaa gttctaatgc tttattgatt
     6301 acatttcgat agccggaaat ataacgaact tggatgcctc ctttttcttc aatgaatctt
     6361 tgtagatgag aagaagtagg ggaattcgtc acgattcttg ttcctggatg ctcttttaat
     6421 acgattgtac ttaatactgc gatcaaatta tttcgattga tcactgttcc agaagcactg
     6481 acaactgcag aacgatccac atctgtatcg aaaatgatcc caagatctgc ttcatttgct
     6541 aaaacagcct gcttgatact ttccatcgct tctttattat caggatttgg gatatgatta
     6601 ggaaatcggc catctggttc caaaaattgg gaacctgttg aatccgcacc taagctattc
     6661 aatacttttt cagcaaagaa tccaccagca ccatttccgg catcaacaat gattttccaa
     6721 ccatttagtg gtttgctttc tagtgttcca atcccctttt taatttttgt gactaaatct
     6781 tgcgcataag gtgtcaaaag gtctgctttt tctacgcttc cagtttgttc gctttttatt
     6841 ttttgctgct gtgataggat aaaagcaata tcttcttttt ctgctcctcc ttctgaggta
     6901 aagaatttta ttccattata ataataggga agatggctag cagtcagcat aaccgcagca
     6961 tcgcacgaga actgttcaaa ttgagtggac ataaacatag caggcgtagt agctaattcg
     7021 aaatcgatga catgtatccc ttgttctttc agactttgga tcaacgcctc tttcagtgct
     7081 ggtccgctta tcctactgtc atgaccaatc ccaatcgtcc attttttatt tggatcggtt
     7141 gatttcttct ttttctttat ccagtttgct agtccccagc cgatcttttt taccatgtct
     7201 gtcgtcaagt ttacttcata tccttcaact ggtaatgcga ttcctcgaat atccgatcca
     7261 ttttgcagcg ctttcatatc tttcatataa aagtcctcct catttattcc ctcatttttt
     7321 ccagcttttc tgcaaatcgc tgtgcttctt cttctgagcg gcaaataata atgaatgtat
     7381 ctacaccggc catcgttccg acaacttcag ggtaagccac ttcatctagc caattcgtta
     7441 ccacatcggc accatttcgt tcagtcaata cgataaccat gaactgaaca gactggatac
     7501 gcaaaacctc gcgtctaatt gctgtacgca aacgttcttc atttaaagaa acagatggct
     7561 ggttaaataa tgtatagcgt actattttgt tttcattttg actttttact aacttcattt
     7621 ctcgaatgtc tcgagaaatc gttgcttgag tcacatcggc gcctgcttca tgcagccgcg
     7681 caagcagctc ttcctgtgtc ccaataggtt gttctgtaat gatctggcgg atcagtgctt
     7741 gtcgctgttt ctttttcata ttagacacct ctcaatgttg attatagcaa aagataagta
     7801 atatttcttc atattttggt attttttact tttatttttt gttttcattc aggttgctaa
     7861 cctttttcta ttttaacacg caaaatcgtc ttcatttttt caggtgctga ttttcttgga
     7921 tcgctgatgt aaatctcttt gtgttctttg gaagctcttt catagccttc ttttgcgatg
     7981 aatgcttcta atttctcaaa cgttgctggt tcttcggcat agggcccgac gtgtaagatt
     8041 tgggcacaaa gaccttcttc catctttttg aatactactt gttctttcaa gtcttccgga
     8101 agtttgctag aacgctgaat ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt
     8161 cggatcatca attgataaga aaaatgttct tttttcatca ctggttcatg caaatacttt
     8221 tcttgcagtc cccattgtcc ttccagtgga tagacagtat acatctgata atcaggaatc
     8281 agccaatttt ttttaggcga catgcgtatt gcatagctga ctgcatatag acaggctact
     8341 cttcgtttaa agtcttcctg attcgggtcg ccgatccctc tgatgctaaa gaatgatttt
     8401 ttaggaacct ctagtatttg cgggcttttt ttcatactat agctcttgtc tttacgccat
     8461 tcaatttttt ccatttttct tcctccgcga aattccattt agttatttat tatcatacct
     8521 ctttttgagt gacttggcta atcgtgttga cactattttt cagtttgtgc tagaataagt
     8581 gaagtaaata aatcgtacag ataacacgtt tcggcgtgtc gagagagagg cggtttctat
     8641 ttttataata gaaatcactc tcttttttta cgaaattttt tagaggtgat gtaaatgaca
     8701 aaacaaaaac agctgctttt agtacctgca gtaattgcaa cagggatgat gtcttttgcg
     8761 ggcgtattga tcgagacggc catgaatgta actttcccaa ctcttatcag acagtttgat
     8821 ttaacgactg cacaagtcca atgggtaaca acgatttatc ttttgatgat ttcgatcatt
     8881 gttccgctgt ctacttattt gaatcgcaat tttagtctca aaaaactctt tttagcttca
     8941 aatctgcttt tcttggcagg tataatgatc gatttcttct ctcctacctt catggtccta
     9001 ttgtttggtc gattgctcca aggtgcagcg acaggtatcg ctcttccgtt gatgttccat
     9061 attatcttga catttgcccc cctcaatcga cgaggcgcga tgatgggtgt cggtactttg
     9121 acaacggcca ttgctccagc aattggcccc acttatggag gcattatgac ttcacatttt
     9181 acatggaatc atatcttcct gtttcttgtg cccgttttga ttctttcttt gattatcggc
     9241 tggtttgcta ttcccgagat ggcagtacaa aaaagtggtt atttggattg gataagcgtt
     9301 ttaggaattt gtcttttgtt tagtggaagt ctaacatttt tgagtacgtt gggaacagtt
     9361 tccagctgga ttgctttaat catcggcggt attggctggt tcctttttta ctatcgaagt
     9421 aaaaaagtgg agcatccact gatccgactg tctattcttt caaataaaac attccgcttg
     9481 tttttgattg gcttcttagt ttatcagttt ttattacttg gtgtatcttt tgtcttgccg
     9541 aatttccttc agatcgttca aggaagtagt ccttttgtcg ctggattagc gatgcttccc
     9601 ggcgcagcgg ttggtgcttt gctttctcct ttttccggga aaatgctgga tcaatatggt
     9661 ccgaagaagc caatttttgc tgggctgatt ctttctttga ttggttggct agcattattc
     9721 ctactcatag gacacgcttc agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc
     9781 ggtatcggat tatcttacag caatatgatg accaccggaa tgaatgcact gaatgaagaa
     9841 ctacaaggag acgggaacgc aatgttcaat accttgcagc agttttctgg cgctgtggct
     9901 acttcattag tagcggtcat tatcaattac gtccagcatc atacttccca taactatgaa
     9961 gtgtctacta cattaggatc aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta
    10021 agctttgctc gattcgccta ttatctgttc tttgctaaaa aagcgtagat tccatatacg
    10081 gcaggtgttg catctgccgt ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt
    10141 ttatggaaaa aattgccatt atcggtggcg ggatcattgg tatgacccta gccagttact
    10201 tagatacatc aaaattcaat gttgctttat ttgatgaagg gacaggacag gcaactaaag
    10261 ctagcgctgg gattatctcc ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc
    10321 tggctaaaga cggcgctgct tttttcccta aacttataaa agatttccaa cttgatcaaa
    10381 cgatttacgc acaatcaggg acacttttgc ttcgacctga aaaagattta gctgaattgg
    10441 ctgttcttgc cgagaaacga aaagaagaag cgccagaaat tggaaacatc gaatggcttt
    10501 cacctgaaca gacatctcaa gcacttccgt tattgaaacc agcagtttca ctgcgtatca
    10561 gtggtggtgg tcgtctggat ggaaaagcat atttagaaca tcttggtcag cgtttagcac
    10621 aaaaacaaat ggagcagatc caggcaaaag tcactttgca taagaagagc gattcttggc
    10681 tcatcaaggg accgtttgga gaatacacag ctgatcgtgt aattttgaca cctggcccgg
    10741 cacttaagcc tttattacaa gaattaggct atgctgcaga tatacgtccg caaaaagggc
    10801 agctcttagc atttcagaca tcttataaga tgagtcaaga ctggcctgtt gctatgcttg
    10861 acggagaagc tgatttcatt cctttcatgg atggaaagat ccttttaggt gctacccatg
    10921 aaaacgatca aggctgggat ttggctccta ctagcgaagc ttataaggga ttgactaaga
    10981 gtacagaaca gttcttgact gatccagatc agataaaaaa ctggccatac gagttccgtg
    11041 taggtacccg tgcttatacg tcagattttt ccccattttt cggcgtactg ccagatgatc
    11101 caagcttact ggctgctagc ggtctaggct cttctggctt gaccacaggt ccttatatcg
    11161 gctatctatt agccaacttc ttaaatactg gtcattggcc tacggaagat tatcaaaagg
    11221 acatctccac ctatattacg aaaaatccat catagaaaaa cagctttttc aagtaaggat
    11281 cccgagcgat attgcgcatg gattacttct tgaaaaagct gttattttag ttcaatttct
    11341 gaatcaaatt ctccaaactt tcttttgctt catctggcaa gagatctttt cctatctgag
    11401 cttcaatgaa tcgtaaacga atatcaagtc gttgtttcac cagattttta taggacgtat
    11461 cctcaaaaga aacaggaaaa tctggtatag gtaaatagtg gatgtcttca aatggaccaa
    11521 acgacttgaa ttctgccgtt tgttgcacga ttctttcaac gattgacaat gtcgtttcag
    11581 gtttgatttt tttatcatcg aaatatccag tattcaggac atagcaggct gtttgctgtt
    11641 tcaaaaacaa ctctcgaaaa gcttgatagt cttccctaag aggatagaca cggaaaggat
    11701 tagcaaatgg ttcaatgact agctcatcta gctgtctttt tgtagtgata ttttccgcgt
    11761 ctgatcgttt tgttgctaat gttgcaccaa aagacgctgc taaaactggg tcattgatct
    11821 tgataattgg cggaagcgaa tcatctttca tgatccaaaa aacagcatcg atcttttcgc
    11881 taagatgatc gactcggtcg ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt
    11941 ttcgtatatc tcctgttact aggacttttt ctcctttact tgtacatgtc acgcccacat
    12001 tctgacaagt aaggaaagaa gaaatggccg gatcatcaga cggatagtct tgtgttttat
    12061 caaagtaaga aggttccaag gcagtagcaa aaccattttt tcgagaaata acgaaagcat
    12121 cgtcatgcaa gatcgaaaca gagtgacctt ccgtatgttt tgctaaggta atcgttgatt
    12181 ttccagaacc ggacaaccca aatacagcca tcgtataggt ctggtcatta ctagcatatt
    12241 gtttcatccc accgtggcag gcaataaagc cattgcgatt tcccaatgcc caagcaagcg
    12301 tcaaagtcgc tttttttaat tctccaaagt aacgtaatcc tagcacagcc gctgtattat
    12361 ggagagcatc aaacaaaatg agtccatttg gaaatgtttc atgtgtccat tcgggatcac
    12421 aaaagatata aagatctccc tcatcaaagg aaacagactt ttgataagtc tctaagatct
    12481 tcgaattcgc tggctgaaaa ttcagcaaat aagaatacaa attgttctca aatccttctg
    12541 gaatcagcaa atgactttgc acagaaaatt cttcagatag gccaacgatt acttttgtat
    12601 ggtaaaactg gcgcttgcga ctttgataca cagcttctct taaaattttc tcgtatttgc
    12661 tttgttcctt gtctaagtcc tcaaagatcc gccgagcact tgctgttctt cccacaatgc
    12721 ttccatcatt gaatactagt gtcctaacat cagctggtaa atctagttct tcagcatgtg
    12781 agattgttaa atctgtgatg atcgttcctg gactcccttt tgctagttga taagctgtct
    12841 tcctatcaaa aacttcatga acattattac cgtaaaatgt cgtttctaca atcgatcgaa
    12901 tactggataa cagtggattt ccacttgtga tttcgtttaa ttcgagactc tcttttgtac
    12961 tcatggctcc tcctcattcc ctaaacaaaa aaacgttttc tttattatac attattcgag
    13021 tttttatttg tactgctgcg ctgtgggaag caaacgagtt atttttcaca caaaaaaacc
    13081 gagaacttga ataaacaagt ttctcggttt catcattttc cttttatcgg cttagtacca
    13141 gccgtttgct aaccagaatg ttttagcagc atcccatgaa ccataacgag atgttacata
    13201 ttgttctgct acacgttctt gattggcagc tgaatagtcg ccgtttaagt atgaagcatc
    13261 taattgataa cggccgatgt aacgtccgtt agtagctgtg tatgaaccgc ctgattcttt
    13321 ttgtgcgatc cattcttttg cagagcttga agttggcgct gttgcagcag ctggggcagc
    13381 aggagctact tcaactgttt caacaggtgc aacgtatgta tgttcttgcg ttgctgtttc
    13441 agttgtagat gctgtatttt ctgttgtttt gttttctgtt gttgctgaag aatcatttga
    13501 agttgggatt gttaattgtt caccaacata aatgcggttg atgtttgcga tcgaattttt
    13561 ttcagcaatt gcatcgatca ttgagttgtc tcctgcaaat ttttgtgaga tcttagataa
    13621 tgaatcacct gattttactg tataaacttc atcagcgtga gcatttgtag ttcctacaaa
    13681 cattgcagta gcaccagcag caaaagttgt acctaa
//
LOCUS       DAGCWU010000065        13619 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.065, whole genome shotgun sequence.
ACCESSION   DAGCWU010000065 DAGCWU010000000
VERSION     DAGCWU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.065"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            46..288
                     /gene="yaaA"
                     /locus_tag="MBZ71_001502"
     CDS             46..288
                     /gene="yaaA"
                     /locus_tag="MBZ71_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBT4429463.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            275..1399
                     /gene="recF"
                     /locus_tag="MBZ71_001503"
     CDS             275..1399
                     /gene="recF"
                     /locus_tag="MBZ71_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBT4429464.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1396..3342
                     /gene="gyrB"
                     /locus_tag="MBZ71_001504"
     CDS             1396..3342
                     /gene="gyrB"
                     /locus_tag="MBZ71_001504"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBT4429465.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3362..5833
                     /gene="gyrA"
                     /locus_tag="MBZ71_001505"
     CDS             3362..5833
                     /gene="gyrA"
                     /locus_tag="MBZ71_001505"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBT4429466.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6069..6368
                     /locus_tag="MBZ71_001506"
     CDS             6069..6368
                     /locus_tag="MBZ71_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBT4429467.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6418..6930
                     /gene="ssb"
                     /locus_tag="MBZ71_001507"
     CDS             6418..6930
                     /gene="ssb"
                     /locus_tag="MBZ71_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4429468.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6956..7192
                     /locus_tag="MBZ71_001508"
     CDS             6956..7192
                     /locus_tag="MBZ71_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBT4429469.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7335..9308
                     /locus_tag="MBZ71_001509"
     CDS             7335..9308
                     /locus_tag="MBZ71_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBT4429470.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9314..9766
                     /gene="rplI"
                     /locus_tag="MBZ71_001510"
     CDS             9314..9766
                     /gene="rplI"
                     /locus_tag="MBZ71_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBT4429471.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10040..11407
                     /gene="dnaB"
                     /locus_tag="MBZ71_001511"
     CDS             10040..11407
                     /gene="dnaB"
                     /locus_tag="MBZ71_001511"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBT4429472.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12187..13479
                     /locus_tag="MBZ71_001512"
     CDS             12187..13479
                     /locus_tag="MBZ71_001512"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBT4429473.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4536 a         2466 c         2998 g         3619 t
ORIGIN      
        1 ggtataatag agtatacttt taataatgaa aatgagggat gggcattgaa acaacaagtc
       61 attctaaata cggaattcat gacgttgggc caaatgttga aagaagtcac agttatcggt
      121 agtggcggac aagcaaaatg gtatttggca gaaaacactg tgttagtcga tggcgaacca
      181 gaaaatcgtc gtggacgcaa actttatccc ggcatgatgg ttgaagttcc agaagtagga
      241 acttttttta tggtaaaaaa aggaaacgac gataatgagg ctgaataagc tgtatttaaa
      301 aaattatcga aattacgaag agctgaacat cgaattttcc aagaacctcg tcatcttttt
      361 aggagaaaat gcgcaaggaa aaacgaatct tttggaaagt atctacgtat tagctatgac
      421 gcgaagtcat cggacaagta gtgagcagga attgattggc tggactgacg atcaggcgat
      481 gattcaagga gagatcacaa aaggttcatc cactcttcca ttagaaatct tattatctaa
      541 aaaaggccga aaaaccaaga tcaaccatat cgaacagaaa aagttaagta cttacgtggg
      601 acaaatgaat gtcattttat ttgcaccaga agatctttct ttagtaaaag gcagcccgca
      661 gaccaggcgg aagtttttag atatggaatt aggccagatc aatccaatct atctttatga
      721 tcttgtccaa taccaagcaa ttttgaaaca aagaaaccag tacttgaagc aattgaatga
      781 aaaaaaacaa actgatttgc tttatttgga tattttgtcc gaacaactag cagcgtttgg
      841 cagcaaagta ttgaaagcaa gagcacagtt cgtcaagcga ttggagtttt gggctaattc
      901 tttgcatcag caaatcaccc atcaaaaaga gcagctggaa atcgagtatc tcaccgcagt
      961 cgattcttta gaaacacata cgcaagagca aatccaagaa caatttctcg cacttttgaa
     1021 ccaaaataaa aagaaagacc tttttcgggg aacaacgaca gttggtcctc atcgtgacga
     1081 tctttccttt tttattaatc agaaaaatgt gcagacttat ggatcgcaag gacagcaaag
     1141 aacaactgca ctaagtgtca aattagcaga aatcgatttg attaaagaag agacagggga
     1201 atatccaatc cttttgttag atgatgtgat gagcgagctg gatgataatc gtcaactcca
     1261 tctgctagaa acaatcgaag gaaaagttca aacgttctta acaacaacga cacttgacca
     1321 tgtaaaaaat aaaatgactg ttgaacctga gattttttat gttcaacaag ggaaactaaa
     1381 aggagaagaa actgaatgac agaagaaaga agtttagtag aacgcgctaa agagtatgat
     1441 gccagtcaga ttcaagtact tgaaggactt gaagccgttc gtaaacgacc tggcatgtat
     1501 atcggttcga ctagttctga gggtctgcat caccttgttt gggaaattgt cgataattcg
     1561 atcgatgaag tattagcagg ttttgcaacg aaaatccatg tgattattga aaaagacaac
     1621 agtatcaccg ttattgacga cggtcggggg atacctgtag atatccaggc aaaaactgga
     1681 cgtcctgcag ttgaaacggt ctttactgtt ttacatgctg gaggaaaatt cggcggtggt
     1741 gggtacaaag tttctggtgg acttcacgga gtaggatctt ctgttgttaa cgcgctttct
     1801 acacagcttg atgtcaaagt atacaaagaa ggaaatgtgt actatcaaga atatcgtcgt
     1861 ggagctgtag tggacgactt aaagattatc gaacaaacgg atcgccatgg aacaacggtt
     1921 cactttacgc cagaccctga aatttttaca gagacaactg agtttgattt ttcaaaattg
     1981 gctacccgga tccgtgaact tgctttcttg aaccgcggga tgcgtatatc gattgaagac
     2041 aaacgagaag aagaaccagt catcaatgaa taccattatg acggcgggat taaaagttat
     2101 gtcgaatact tgaatgcaaa caaaaccgtg atttttccag aacctgtata tttggaaggt
     2161 gaacagcaag atatcgcagt tgaagtctct atgcaatata cagacggcta ccattccaat
     2221 atcatgagtt ttgccaacaa tatccatacg tatgaaggcg gaacacatga atcaggattc
     2281 aagactgctt taactcgtgt gataaatgat tatgcccgaa aacaaaagct gatgaaagaa
     2341 aatgacgaca acttgaccgg tgaagacgtg cgagaaggat tgacagctgt tatttctatc
     2401 aagcatcctg accctcaatt cgaaggacaa acaaaaacaa aattgggcaa ttccgaagta
     2461 cggacagtca ctgatcggtt atttagcgaa catttcatga aattcttgct ggaaaaccca
     2521 agtgtgggcc gtcaaattgt tgaaaaagga ttactagctt ccagagctcg tctagcagcg
     2581 aaacgtgcgc gtgaagtaac gagacgtaaa ggtgctttag agattagtaa tctgccagga
     2641 aaattagcgg actgctctag caacgatcct gaaaaatgcg aattgttcat cgtcgaagga
     2701 gattcggccg gcggatcagc taagcaaggt cgtaatcgag agtttcaagc catcttgcct
     2761 attcgtggga aaatcttgaa tgttgaaaaa gcaagcatgg acaagatctt ggcaaatgaa
     2821 gagatccggt ctttatttac tgctatgggt acagggtttg gagaagattt tgatgtatcg
     2881 aaagctcgct accacaaatt agttatcatg actgatgccg atgtcgacgg cgcgcatatc
     2941 cgtacattgc tgctgactct tttttatcgt tacatgcgtc ctattgttga agctggatac
     3001 gtctatattg ctcagccacc tttatacggg gtaaaacaag ggaagaatat tacgtatgta
     3061 caaccaggaa aaaatgcaga agaagagttg aaacaagtcg ttgcttcact tcctgcctca
     3121 ccaaaaccaa gcgttcagcg ttataaagga cttggagaaa tggatgatca tcaactatgg
     3181 gaaacaacta tggacccaag caatcggatg atggcccgcg tcagtgtgga cgatgctatt
     3241 gcagcagatc agatatttga aatgttgatg ggggatcgag tagaaccccg tcgtgcattc
     3301 atcgaggaaa atgcacacta tgtgaagaac ttagatattt aatcatgaag gaggaaaaag
     3361 aatgagtgaa gaaatcagag aaaacatcca tgatgtcaat ctaaccagcg aaatgaaaga
     3421 ctccttcatc gattatgcga tgagtgttat tgtcgcacgt gcacttcctg atgtcagaga
     3481 tggtctgaaa cccgttcatc gccgtatttt atacggcatg aacgaattag gtgtaacacc
     3541 ggataaagcc cataaaaaat ctgccagaat cgtcggagat gtcatgggta aataccatcc
     3601 tcatggagac tatgcgatct atgagtcaat ggtacggatg gcacagccat tcagctaccg
     3661 ttatatgcta gtggatggtc atggaaactt tggttctgtt gacggtgacg gtgccgccgc
     3721 tatgcgttat accgaagcac ggatgagcaa aatcgctaca gaaatgctgc gtgatatcaa
     3781 taagaacact gttgatttcc aaagcaacta tgatgataca gaaaaagaac cagttgtact
     3841 acctgctcgc ttcccgaatc ttttagtgaa cggaacaacg gggatcgccg taggtatggc
     3901 cacgaatatc ccacctcata acttgtctga ggtagtagcg gctatcgatt tgctgatgga
     3961 taatcctgat gtgacgacca atgaattgat ggaagtatta cctggaccag attttccaac
     4021 aggtggtttg gttatgggaa aatcaggaat ccgaagagcg tatgaaacag gtaagggttc
     4081 tattaccgta cgtgcaaaag ttgagattac tgagatgcca aatggtaaag aacggatcat
     4141 cgtgacagaa ttgccttata tggtcaataa agcaaaattg atcgaacgga tttctgaact
     4201 gcacagagac aagcgtatcg aagggatcac agatttgcgg gacgaatctt cccgtgaagg
     4261 tatgcggatc gtcattgaca tccgtcgtga cgccagcgca tctgttatct tgaataactt
     4321 atataaattg acatccttgc aaaattcatt tggattcaat atgttggcga tcgaaaaagg
     4381 tgtgccgaaa gttttgagct tgaaacaaat tcttgaaaac tatatcgaac atcagcgcga
     4441 agtcatcaca agacggacag tctttgaaaa agaaaaagca gaagcaaggg cacacatttt
     4501 agaaggtttg cgtattgctt tggatcatat cgatgaaatc attgcgatta tccgcgggtc
     4561 taaatcagat gacgaagcaa aagcgacgat gatcgaacgg ttcgatttat ccgatcgcca
     4621 gtctcaagct attttagata tgcgtttacg ccgattgacc ggtttggaac gggaaaaaat
     4681 cgaaaatgaa taccaagaac tcttaaaact gattgaagat ttaactgaca ttctggcaag
     4741 accagaacgt gttacagaaa tcatcaagaa tgaattagca gacttgaatc aacgatttgg
     4801 tgataaacga cgcacagaat tactagtcgg tgaggtctta agtctggaag atgaagattt
     4861 aatcgaagaa gaagaaatcg ttattacatt gacaaacaat ggttacatca aacgtttggc
     4921 aaataatgaa ttcagagctc aacgaagggg tggacgtggt gtccaaggaa tgggtatcca
     4981 caatgacgac ttcgtcaaaa atctggtttc ttgttctacc catgatacat tgttattctt
     5041 tacgaataac ggaaaagtgt atcgggcaaa aggatacgaa attccagaat acgggcgcac
     5101 agccaaagga ataccaatta tcaatttact aggtatcgat tcgagtgaaa gcatccaggc
     5161 aatcattgcg gtgacgggag aagcagaaga aggccactat ctcttcttta cgacgcgtca
     5221 aggtacagta aaacgtactt ctgtcactgc ttttgctaac atccgaagta atggtctgat
     5281 cgcaattggt ttgaaagaag acgatgagct cgtcaatgtg ctactgacag atggtcaatc
     5341 taatatcatt attggaacac ataacggcta ctctgtcact ttcaaagaag aagcagtccg
     5401 cgatatggga cgtacagctt ccggggttag aggaatacgc ctgcgtgagg aagattatgt
     5461 cgttggggca gccttaatga aggaagatca agaagtatta gtgatcacag aaaaaggtta
     5521 cggaaaacgg acaaaagtat cggaatatcc agtgaaaggc cgcggaggta aagggatcaa
     5581 aacggcgaac atcactgaaa aaaatggtcc actagctgga ttgaccactg tttctggaga
     5641 tgaagacatc atgctgatta cagataaagg tgtcattatc cgtttcaacg tatcgactgt
     5701 ttctcaaact ggacgttcaa cattaggcgt acgtttgatg aagatggaag ctgatacgaa
     5761 agttgtcacg atggcagcag tcgaaccaga agctgcagaa caaacagttg aagaacaagg
     5821 atcagaagaa tagccatcaa gactaattgt gtaagctagc aagaatgacc gattaaaaaa
     5881 tctggttatt cctgctggct tttttgatta gttgaatttc tcttaaaata ttcgaaactg
     5941 ttcattctgt attgcttttg accaaaatag agtgtataat cagagcttgt gagtagtcta
     6001 attaattaga taactctcct tgctcttagg aacctaagag ccgaaagtcc acagggaggt
     6061 gaatatcaat ggaaaacacg aaatatgaaa tcatgtacat tattcgtcca aacattgatg
     6121 aagaagcaaa aactgcttta atcgaacgtt tcgatgcaat cttgaaagat aacggagcag
     6181 aagttatcga atcaaaagat tgggaaaaac gccgtttcgc ttatgaaatg aacggttacc
     6241 gtgaaggtat ctatcacatc gttaaagtat cttctccatc atcagcaaat gctgtcaatg
     6301 aatttgaccg tcttgctaaa atcaacgacg atatcattcg tcacatgatt gttaaagaag
     6361 aagcttaatt gttgtttcat gtgaaacaat agaaaacgat gagaggagat gactattttg
     6421 attaataatg ttgtattagt tggacgtctg acgaaagatc cagatttgcg ttacacagca
     6481 agtggtacgg cagtcgcaac atttactttg gctgtgaatc gtaattttac aaaccaaagc
     6541 ggcaatcgcg aggctgattt tatcaactgt gtgatttggc gcaaatcagc agaaacttta
     6601 gcaaactatg ctcgtaaagg aacactttta ggtgttactg gacgtatcca aactcgttct
     6661 tatgataatc agcaaggaca acgtgtatac gtgacagaag tagtagctga caacttccaa
     6721 ttgctggaaa gtcgttctgc atctgaaaat cgccaacaaa gcggcggatt ccaaagttct
     6781 ggtcaatcag ctggcggatt tggcggaaac aataactcta atcaaacatc tcaatcatcc
     6841 aacggtatgc ctgatttcga ccgtgatact tctgacccat tcggctcaag ttcaacgatc
     6901 gacatttcag acgatgattt accattctaa acgaatttga taggagggaa atagaatggc
     6961 acaacaaaga agaggcggac gtaaacgtcg taaagtagac tacattgctg ctaaccatat
     7021 tgaatatatc gattataaag atatcgaatt actaaaaaga tttatttcag aacgcggtaa
     7081 aatcttgcca cgtcgtgtaa ctggtacagg cgctaaaaac caacgtaaaa ttactgttgc
     7141 aatcaaacgc gctcgtatca tgggcttgct acctttcgtt agcgaagact aataaatcct
     7201 aggaattgaa tgactgatcc ttttgatcag ctattcaatt cctttttttc tgtaaaaata
     7261 acctctattc gtttagtcgc tcgaatgacg aaagttatgg taaaatcaaa ttagtcatat
     7321 atgggaggaa aattatggaa aataaaaaca atcatttatc cccgaaacct ctcttcctta
     7381 tcctgtttat attaatcgaa gtattggtag tcttaatttt accatttcgt tttgttagcg
     7441 cttcggtcat tctattgatg aatttatggg gtgttcgcta ttggttgaaa atgaaacgtt
     7501 ggcttatttt aaataaacgt gaaaaaatcc agcaggcttc gactgtagct gaaaccaatc
     7561 tagcttatgt ctcagaagtc atgcctgtag gagtgattgc ttatcagcca tcaagccaaa
     7621 aaatcgaatg gctcaatcca ttagcattga aaatcaaaga tcaaagtgga ttaacagagg
     7681 aagaattgct agatactttt tttactgcaa aagaaaaaaa gaaaaaccaa gtctatctag
     7741 cagataaaac atttcagttt gatatggata cagtaaaggg tgttgtttat tttaatgata
     7801 ttacccaaaa ccagttgtta aaactccatc agacgaacag tcagccggtg attggtattt
     7861 tatctattga taactatgca gatgccattg ataaattgga tgacaaagag atttcttatt
     7921 tgaactcttt cgtcactaca ctagtttctg actggatgaa tgaataccat gtctttttca
     7981 aaagaatcaa cgcagaacgg tttttcttcg tagcacagat gatcgatctg aaaaagatga
     8041 tggataaaaa atttgatttg ctggatcgct ttcgagcaga agctgaaaaa cagagctcgc
     8101 ctatcaccct tagtatgggg attgcttatg gctcaagaac cctcgatgaa atcggtggag
     8161 aagctcagaa taatttagat gttgctttag tccgtggagg cgaccaagtt gtcgtgaaag
     8221 aggtaaaaga aggagcaaaa cctgtttatt atggggggaa atctgctaag aatgtcaaac
     8281 gcacgcgtgt tcgttctcga gcgatgagta ccgctttgcg tcggattttt gatgaaacag
     8341 gcgatgtgtt tatcatgggg cacaagtttc cggatatgga tgctttaggc tctgcttttg
     8401 gtgtcagttg tctggcacgc ttcaaccaga aaaaagctta catcgttatc aacgagaacg
     8461 agatcattcc agatgtagaa cggtgtcttg aagaaatcca taagcatcct gaattagaaa
     8521 tgcaactaat ctctccagaa cgtgccatag agctgaaaaa agaagaggac ctcctcgtga
     8581 tggtggatta ccataaacct tctctatcga tctctcaggg cctatatgag caatttgata
     8641 agattgtgat cattgatcac catcgtcgtg gggaagagtt tccaagtaaa cctctgctga
     8701 cttatatcga atcttcagct tcttcagctt cagagctagt ttctgagctg atccaatacc
     8761 aaagcagcaa aacaaatcgc ttagatcgtt ttgaggccac attattgctt gcagggatgg
     8821 ttgtagatac aaatagtttc aatgtccgta caactgcacg aacttttgac gttgccagct
     8881 atttaaggtc ttgtggagca gacgcatcct tagtacaata tttattgagc tcggatttag
     8941 cgacatactt ggagatcagc aatttgattg ctggaagcga ctatgtgact tctgatattg
     9001 ttgtggcggc agggacagac gaaaaagagt ataatagtgt tatagcagca aaaacagcag
     9061 atacattgct ttcgatgatc aatatcaatg cagctttcgt cattacaaag cggacagacg
     9121 gcttgattgg gatcagtgcc agaagtaccg gcacgatcaa cgtccaaatc atcatggaat
     9181 ccttaggtgg cggaggacac ttcacaaacg cagctactca actggaaaat acgacagttg
     9241 aggaaacaaa acagaagcta tttgctgcaa tcaaagaaaa tatgaatcaa atatatgata
     9301 aggagtaatc caaatgaaag taattttttt acaagatgta aaaggaaaag gtaaaaaagg
     9361 ggacatcaaa gaagttccag caggttatgc acaaaattat ctaatcaaaa ataacttggc
     9421 aaaagaagca acgaaaagca gtatcagtca gttagcagga caaaagaaag cacaagaaaa
     9481 acacgaagct gaaatcttgg aagaagctaa aaagttaaaa gaattcttgg aaaaagaaga
     9541 tactgtagtc gaattgaagg cgaaagcagg ggaagacagc cgtttgtttg gttcgatccc
     9601 ttcaaaacaa attgctgaag ggttaaacaa acaatacaaa atcaaattag ataaacgaaa
     9661 aatcgaatta gaaaatccga tccgttcact aggctacaca aacgtacctg ttaagcttca
     9721 tcatgaggtg acggctacga tcaaagttca tgtcgtacaa gaataacaaa tcttgatagc
     9781 gatgttcagc tggtaagtat aaccagaagg aaaaaagaca aaccgaaatt tatctgagaa
     9841 aacagctgca gcaatgaaag caaaagctgc aaatcgattt cagattgatt tcggtttctt
     9901 ttttgggaac agcaaaatag cataaagaaa acgagtataa taaaagagaa ctattttttt
     9961 attacacttt cggtacgaat gatgactttt ctctcatttt cttgtaaaat gaagcaattg
    10021 aagacaagga gcgatacgta tgaatgaagt gtggcaggat cgaatcccac cgcaaaatat
    10081 tgaagctgaa caagcggttt taggctctgt ttttcttgac gcagaaacga tcattgatgc
    10141 catggagtac atagagccaa aagactttta tcgtcgtggc catcaaatta tttttgagac
    10201 aatgatcgag ttaaatgatc gtaatgaagc gattgacgtg gttactgtca aagaccggct
    10261 ggaacaagcc aatttgttag aagatgccgg tggtttaagt tatctctcag acttggcatt
    10321 agcagttccg acagcagcaa atatcgtcta ctatgcgaaa atcgttgaag aaaaatcatt
    10381 gctgcgtaca ttgatccaga cagcaacagg aatcgttacg aaaggattcg agcaaggaga
    10441 agaagttcaa acaattttag atgatgccga acgcagtatc ttggaggttt cggaaaaaag
    10501 aaatcgaagc ggctttttat ctattgctga agtactgaac tccacgattg aaaatatcga
    10561 tcgactagct caaaacaatg aagaaatcac aggtctacca actggctatc aaggtctgga
    10621 taaaatgaca gcaggtttcc agcccgaaga actaatcatt cttgctgcac gtccagcggt
    10681 aggtaaaaca gcgtttgcat tgaatattgc tcaaaatgta ggaacaaaaa cagatcgttc
    10741 tgttgcgata tttagtttgg aaatgggggc agaatctctt gtaaatcgga tgctttgtgc
    10801 agaaggttcg attgaggcca gccatttacg tacaggtcag ctttcggaag aagaatggtc
    10861 gaatctgatc gttgcgatgg ggagtctttc tcgagcaaat atttttatcg atgatacacc
    10921 agggatcaaa atctctgaaa tccgtgcgaa atgtcggaaa ctagcgcaag aaaaaggcaa
    10981 tctaggcttg atattgatcg attatttgca gttgattgaa ggtaacggta aagagaaccg
    11041 tcaacaagaa gtatcagata tctctcgcca actgaaaaaa ctggcgaaag agctgaaagt
    11101 acctgtcatt gctctttcac agttatctcg tggagtagaa cagcgacaag acaaacgacc
    11161 agtgctaagt gatattcgtg aatccggatc aattgaacaa gatgcggaca tcgtagcctt
    11221 cttgtatcga gatgattatt atgaaagaga aggcggagat gacgatgatg gaaacgagcc
    11281 aaaaaatgac aacgtcatcg aagtcatcat cgagaagaac cgaagcggtg cacgaggaac
    11341 agttgaacta ctgtttatca aagaatacaa taagttttcg tcgctttcgc caagagaaga
    11401 attttaaaga agaggttgtg agccttgagc aatcccgaga gctctcgtaa gaagatgcaa
    11461 ggagtaaccg caggaacgac tagagtactt aatttaaaca gatctccaaa gggattgtga
    11521 caaaagtctt gtcacagtcc ctttttccga ataaacggtg ttcaatcagc tgacccttgg
    11581 tattagctca gcaaacgtca aaatatgagg aactattttg acgtttgttt ctctaatact
    11641 caggtcaaaa cgctgatctt acgacctctt ttccttaaac ggtgttctcc tgccaactcc
    11701 tcggtcacaa gtcacaatat catcaaacat gagaagcagt tttttgaaat tgctccttgt
    11761 gactcagagt taaacggcag gctcacaacc tctttcttaa acggtgttca aaagcttgca
    11821 tctgcggtaa tacgcccaat caaacgaaaa atatgagaag ctatttttgt ttggctgttc
    11881 ttattacttg atgcaggaca gctttttcac aacctctgat tcacggtgtt ccaaaagtag
    11941 cttcttcgaa aaataagtcg aaatttcaca aaaatttgag aagcaatttt cgtacattcc
    12001 ttcttatttc ttgaagctga ccacttctgt tacaacctct tttttaataa ccattctaaa
    12061 aagataaagt tcgtttttta agcgttttta taaaataaaa ataaaaatca ttcgatttta
    12121 tttgaaatat gtatctatta ttgatacaat ggttcaggat taaataatat agaacgaggt
    12181 gttcacatgt catctgtagt agtagtaggt acgcaatggg gcgatgaagg gaaaggaaaa
    12241 atcacagact ttctcagtga aaatgctgaa gtgattgcac ggtaccaagg tggcgacaat
    12301 gcagggcaca caattaagtt taacggtgtg acttataaac tgcatttgat tccttctggg
    12361 attttttata aagacaagat cagtgtgatc ggaaacggcg ttgtagtcaa tcctaaatca
    12421 ttggttaaag aattagctta tttacatgaa aaaggtgtag aaacaagtaa tttgcgtatt
    12481 tctgaccgcg cgcacgtaat cttgccttat catatcaaat tggaccaatt acaagaagat
    12541 gctaagggcg aaaataagat tggtacaacg attaaaggaa tcggacctgc ctatatggat
    12601 aaagcagctc gtgtaggtat ccgtattgca gacttattag ataaagaaat ttttgaagaa
    12661 cgtcttcgtt tgaatctaga tgacaaaaac cgtcaatttg taaaaatgtt tgattcagaa
    12721 ccacttgatt ttgacgatat ttttgaagaa tattatgaat acggacaaca aatcaaacaa
    12781 tacgtcacag atacttccgt tatcttgaat gatgctttgg atgcaggcaa acgcgtgttg
    12841 tttgaaggcg cacaaggtgt aatgctggat atcgaccagg gaacttatcc ttttgttact
    12901 tcttctaacc cagtagccgg tggggtaaca atcggaagcg gtgtagggcc ttcaaaaatc
    12961 aataaagttg tcggcgtctg caaagcctat acgtcacgtg tcggtgatgg accatttcca
    13021 actgaattat ttgatgaaac aggagaacaa atccgcgaag taggacgaga atacggtacg
    13081 acaactggtc gtccgcgtcg tgtaggctgg tttgattctg tagttatgcg ccattctaaa
    13141 cgagtttcag gtattaccaa cctatcttta aactcgatcg atgtattaag tggattggaa
    13201 acagtaaaaa tttgtacggc ttatgaatta gatggcgaac taatctacca ctatccagca
    13261 agcttgaaag agttgaaccg ctgcaaacca atctatgaag aattgccagg ctggtcagaa
    13321 gacattacag gatgcaaaac acttgcagaa ctgccagaaa acgcacgtaa ctatgtacgt
    13381 cgtatctcag agcttgttgg tgtacgtatc tcaacatttt ctgtcggacc tgatagaaca
    13441 caaacaaaca tcttagaaag cgtctgggcg caaatctaag gataaaaacg acgactgaat
    13501 caggacgttc ttaccagagc gagtcgaagt gtttatctat acgataataa taacttgaac
    13561 actgtttatt cagaataagg gactgtgaca aaaaccgtgt cacagtccct ttttccgaa
//
LOCUS       DAGCWU010000066        13311 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.066, whole genome shotgun sequence.
ACCESSION   DAGCWU010000066 DAGCWU010000000
VERSION     DAGCWU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.066"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            266..2146
                     /locus_tag="MBZ71_001513"
     CDS             266..2146
                     /locus_tag="MBZ71_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4429474.1"
                     /translation="MDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNL
                     FWQKKNKGIEMVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFT
                     TQTVKNKEQPTVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMI
                     RNLRIGLVAVSLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQS
                     VSRIIGEKEALSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYT
                     ALVQIDLLEDSHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDA
                     EASKKVMEYCRLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGP
                     SSIIFYQEIVPAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNP
                     LSREVVSVRLINELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLP
                     ITECSQAITDHEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGE
                     KFGDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKK
                     YL"
     gene            complement(2604..3476)
                     /locus_tag="MBZ71_001514"
     CDS             complement(2604..3476)
                     /locus_tag="MBZ71_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4429475.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            3776..4750
                     /locus_tag="MBZ71_001515"
     CDS             3776..4750
                     /locus_tag="MBZ71_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4429476.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            4772..5464
                     /locus_tag="MBZ71_001516"
     CDS             4772..5464
                     /locus_tag="MBZ71_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBT4429477.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            5430..6938
                     /locus_tag="MBZ71_001517"
     CDS             5430..6938
                     /locus_tag="MBZ71_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4429478.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            7012..8115
                     /locus_tag="MBZ71_001518"
     CDS             7012..8115
                     /locus_tag="MBZ71_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBT4429479.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            8118..9455
                     /locus_tag="MBZ71_001519"
     CDS             8118..9455
                     /locus_tag="MBZ71_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBT4429480.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            9442..10320
                     /locus_tag="MBZ71_001520"
     CDS             9442..10320
                     /locus_tag="MBZ71_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBT4429481.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            10317..11240
                     /locus_tag="MBZ71_001521"
     CDS             10317..11240
                     /locus_tag="MBZ71_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBT4429482.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            11237..11647
                     /locus_tag="MBZ71_001522"
     CDS             11237..11647
                     /locus_tag="MBZ71_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBT4429483.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            11651..13219
                     /locus_tag="MBZ71_001523"
     CDS             11651..13219
                     /locus_tag="MBZ71_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBT4429484.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT         4439 a         2197 c         2813 g         3862 t
ORIGIN      
        1 agccgtactt gtcataggat ttagtttatt catttaccag aatacctatg aatcgatcga
       61 atcgagttat ttaacagaca aaaagaattt gctcaggcaa gttaaaacta acgttgaatg
      121 gaaaattaga acgattgaat attcgttttc tacgtatggt tcgaccaaaa atttctcgga
      181 catattcaag catccgctga catatacaga ttactccact tattctgaaa tcaggaaaga
      241 actaaatttt atagaaacga ttgttatgga tgataataat tatgatttag tcagtttggc
      301 tggtaaatgg ggagtaatca atggatcgtt gagtcaactg accgaagagg aaattgaaaa
      361 gtatcaacag aagtacataa ataacaaaaa taatttattt tggcaaaaga aaaacaaagg
      421 aatcgaaatg gtgatttctt tgccaatgtt tgaaaaagaa aaatatgcat tgggaattgc
      481 tacgatagag aaacatacaa tagaacaagt tgttggtgaa agtggagaag acatactttc
      541 tattgaaaac gaagttggca acctttttac tactcagaca gtaaaaaata aagagcaacc
      601 aactgtatta acggacgtgg atctagatta caatcagctg aaagtgataa gagaaaaaga
      661 taaaacgtat atagtactga aatcagatta taataattgg atttacagat tagaagttga
      721 ctctagtgca attggcgcaa tgatccgcaa tctgagaatc gggctagttg cagtgtcttt
      781 aacactggta ggactcattg gtcttctttc ctacttattt tcggacagat ttgcccgtcc
      841 gatttctcaa atacaggaac gtctgaatct taaatcaaaa ggattcagtg ggaaagaact
      901 agatttggtc gctcaatcag tcagtcggat cataggagaa aaagaagcgt taagtgcaca
      961 cttagttacc caaaagccac agctggaaac gctatttgta cttagtttat ttagaaatcg
     1021 agtagaaagg cgagaactag atcagcggtt gcagcaattt ggttatagtt ctcaaaatga
     1081 ctgctattat acagctttgg ttcaaatcga tctgttagaa gatagtcacg gtggggagcg
     1141 tgatttgcta cttttagcga tcaacaacat gatcacggaa attgtgccaa aggaaaatcg
     1201 aatgctgccg atcgttttga atgaagaaat gcaagccact atttatcgaa tggataaaga
     1261 tgatgcagaa gcaagcaaaa aagtcatgga gtattgccga ctcattcaaa aacgtgtaaa
     1321 agaatacttg aatttaacta tcagtatagg catcagtaac cggttcgaaa cgctgaatga
     1381 aagcaagcaa tcggttgatc gggcaaaaga agccctgtat tatcgtgtaa atactggtcc
     1441 aagttcaatt attttttatc aggaaatcgt tcctgctgct catgaaaaaa cacttattag
     1501 atatccgata gaagaacaaa atcgattgtt tgaagccatt cgttctggaa atcaggaagt
     1561 gaagaattta gtacatgaat tgattgatgc attgtttgca caaaataaaa atcctttgag
     1621 tcgggaagta gtttcggtcc gcttgatcaa tgaactcgtt caattaggac agttattggg
     1681 agtagattca aaaaactttg ataacatgaa acaaatttat gtaaaagcac tgaacaatta
     1741 tcatcccaaa gaactagaaa aacttgtttt agaacaatta gttctgccaa tcacagaatg
     1801 ctctcaagca atcactgatc atgaatttaa aaatttgtcg gagaatatta tgcatatcgt
     1861 ctacacagaa tatgatcgtg atctgtcttt agatgtgatc gctgataggt tgcattataa
     1921 ccctaactat ttaagtaatg tatttaaaaa ggaaacagga gaaaaatttg gtgattttgt
     1981 tcaaaatcat cgtttagagg tagcaaaaaa atggttgaaa gagactaacc tctccgtaaa
     2041 agagattgca gaaagattgc aatatcgaaa tccgcaaaat tttattcgtt tctttaaaaa
     2101 gaaggaatcg attacgccgg gagaatatcg aaaaaaatat ttgtgaatca agaggctgtt
     2161 tttcttgtgg actgattcaa gcaataaaag aagttagaat aaccctagta tttgtattta
     2221 tggatagtta aatttcaagg ctaagcacgc aaaaagaact gtgacaaaag tcttgtcaca
     2281 gttctttttt acgaataaat ggcgttcaag agacaggcta tcgacaataa ggcataattt
     2341 tgtgaaatat gggaagctat ttttcaaaat cgcttcttat tgctcctgtg ggttactcgt
     2401 cgcaatgttt gtgagagttt ttatcatcgc tcaaggctga acgtctcttt cacaacctct
     2461 gatacacggt gttccaggag tagattcttc gaaaaataag ccgaaatttc acaaaaattt
     2521 aagaagcaat tttcgtaaat gccttcttat ttcttgaagc tgaccacttc tgacacaact
     2581 accattttgc gttcagtaca taatcaccct ttgacagaac ctacgagtgc tcctttgaca
     2641 aaatatttct gaacaaaagg ataaacaatc agcataggca ctgttgcaat aacgataaca
     2701 gccattttaa ctgtttgtgc tggtggaatc acatcaacgg cactactttc tgcttgcatc
     2761 ccacttgata caatgacgat ttggcgtaaa agaatctgta tcggccattt agatgaatcg
     2821 ttgatataca aaatagcagt catataagtg ttccagtaag taacagcata aaaaagcgag
     2881 atagtagcaa tagacggtaa cgctaatggc agcataattt tgaaaaatac accaaaatct
     2941 gtgcaaccat ccatttttgc tgattcttcc aaactatccg gcaaggcttg gaaaaagttt
     3001 ctcataatga tcatattcat tgcattaaca gccacaggca ggatcatggc ccagtaagaa
     3061 ttgaccagcc ctagagaacg aacaactaag aacgttggaa tcatcccccc gctgaacaac
     3121 atagaaaata caacaagaaa gttgacgaaa cctcgaccaa acaaatactt tctggaaaga
     3181 ccgtaggcca tcagtgaagt tgcacacata ctgacgatcg ttccgacacc tgtaacacca
     3241 atcgaaacag caagtgctct aaaaatggtc ggcgtagaaa gaatgtagcg atacgcgtct
     3301 aaagaaaagg tagtaggaaa aaggataaac tttttcgcga cgacttcttg tgtagaagca
     3361 aaagatgtag cgatgatatt taaaaaagga acgatacaga tcaaagctag caaaattaga
     3421 aacgtattgt tgaaaagtgt aaaacttctt tctcctatag agactttctt tttcatatag
     3481 ataggctcct cctcaaaata aattaaatgt ttctgtttct atatgttcaa tttatcatcg
     3541 gtgaaagatt acgtatataa tcatttccta tgctaagtgt cagatagaaa aaaatagaag
     3601 gtttgctatg cgtagtttat ttgtgaaaaa atacacttta tcgttttgta tagcacaaaa
     3661 atcattgcta tgtaagcgtt tttacttacg gtaaaaatga ttatatacgt aattcagata
     3721 gttatctatc atgaagtcat aagcagcaaa cattcacaaa actaaggagg ggcttatgga
     3781 aagtgtaaag aaacaagaac catttcaact gacgctatcg aaaaaagaag aaaagctcag
     3841 aagacgaaaa aaaataattg caggaatcaa aacgaataaa tttttatatg tgatgattct
     3901 gccgggagtc ctttatttct tgatttttag gtatcttcct atggggggac tcgttattgc
     3961 ttttcaagat tatcaaccat ttttaggaat aatgggaagt ccttgggtcg gtttcaaaca
     4021 tttcattcgt ttgtttacag aaccgacatt ctttacatta ttaagaaata cgttgatctt
     4081 gttcggatta aatatcttcg tgttttttcc gatgccaatc attttgtctt tactattaaa
     4141 tgaagtaacg aacaaacgat tgaaaaacgg gattcaaacg atcatttaca tccctcactt
     4201 catgtcttgg gtaatcattg tttcgattac ttatgtattt ttgaatgttg acggaggggt
     4261 attgaatgaa ttccttgcaa caatgggatt tgaaaaaatc agttttctaa catcccctga
     4321 atggacacgc acagtatata tcggacaagt gatctggaaa gagttgggct ggtctacgat
     4381 catctacctt tcagcgataa ctgcagtcga tacacagttg tatgaagctg cagaaatgga
     4441 tggagcagga cgcttacgca aaacatggca tgttacattg ccggcaattc gtccagtaat
     4501 cattaccttg ctgattctaa aaatcggtca gacattagac ttagggttcg agcatatgta
     4561 cttgttattg aattcattga atcgaagcgt tgcagaaatt ttcgacacct atatttatac
     4621 agctggattg aagaatggac agttaagttt cagtacagca atgggattgt ttaaaggggt
     4681 cgtaggattg tttatggtaa ttttagctaa taagattgcc aaaaagctag gagaagacgg
     4741 cgtttactaa gatagaaaga gagggaaaga tatgctgcag tcattagata aaataaacgg
     4801 aaggctaaat cgtattttac agttggttta tttgaatttt ctgtggggcg gtttagtcat
     4861 cgcaggtttg ggagttttta ctttcggtcc tgcatcgtat gcgatggttg cagtgattag
     4921 aaaatggctg cgctcaaaag aaacgtttcc tttgacgaaa acgtacttca catattttaa
     4981 agaaaactat cgtgaaacct tgctgatgag ctggatatat ggcgccatcg ggattgtttt
     5041 acttgtggat ctttctttca tacacaattg gtatcttagg atcgctttgt atattgctgc
     5101 gttcatatat gtgctctcag cactctatat tttcccaatc atggctcatt atgattggaa
     5161 aggatatttt ttcaaaatga aaatggcatt tttattcgga ttctcacatc ttcattactc
     5221 attagtgttg atattaagta tggtagggat ttatctgatg atcatccaaa caatgccagg
     5281 tgttttaacc tttatcggtg gcagtctctt cttttccgca gtcacgtgga cagcaaattc
     5341 tatattttgc catttggaat ggaagaactc atatacagaa aaaacagaag aaaacaaaaa
     5401 tattattaaa acagtgaagg agaaaattta tgaaaaaagc aacgctcgta aaattaggtc
     5461 ttagttttgc aggaattcta gcacttgccg catgcggcag cagttcaagt aacagttctt
     5521 cagagacaga agatggaaaa gctaaaatca cttggttaaa tattttgcat catgcttcac
     5581 cgcctacaga cacgattgtg aacttgatcg aagaaaaaac cgatacggaa ctgacattca
     5641 actggattcc tgatgcttca aaagaagaaa gattaacaac tgcattggct tctgatgaac
     5701 taggagatat cgttacactt caaattgcga tgttgaaaaa ctcttctgtc cgcaatgcgt
     5761 taaaatcagg cgtattctgg gatgtatcag aatatttaaa agactataaa aatttaagtc
     5821 aaatttctga agatagaatc gaagctgcga gtatcgacgg tgccttgtac ggcgtaccga
     5881 tacaaaaaga ctacgctcgt gctggcttag tcattcgaaa agactggttg gataatctag
     5941 gactagatgt tccaactacc atggatgagc tttatgaagt agctaaacaa tttacagaaa
     6001 acgatcctga tggaaatggt gtggacgaca cagttggttt tggcgatcgt gcgcctagtg
     6061 atattcgtta taccagcttt aaacttttca cttcttattt tggagcacca aatggctggg
     6121 ctgtagacga taatggcaaa ttcactccgg aatttgatac agaagagtat atgaaagctt
     6181 tgaatttttc aagagatcta tttgagaatg gctatttggc acaagacttt gcagttaccc
     6241 aaaagactga tcagcaagaa caatttgcac aaggaagaac tggaatctac actggaatgg
     6301 tggatatcaa gaatctgaag acgatgggtc aaggtattca agatgatatg gaattagtgc
     6361 ctgtaaacaa gatctcagat ggtgaaggcg gcggttatca tgtttggtca gaaggaaatg
     6421 gtattggcgg cttgctagca tttcccaaat ctcaagtaaa agacgaagca caattgaaga
     6481 aattacttca atttgttgat gatcttttag aagaagatgt gtataaattg atgactggcg
     6541 gtatcgaagg aacgcattat gaattgaatg atgatggttc tatcgaattt atcgatatgg
     6601 atctttggca acaagaggtg caaccgttat caagctctcg tcctagtgaa gtgacgttct
     6661 ctttcaaaga tacagaccct gaaaaagaat tctctaatca attgatcaaa gaaaatgaag
     6721 aatttgctgt aatggatcct tctgtgccat tggattcacc gacgaacaat gaagtaggat
     6781 cagaaattga aaaaatcgtc attgatgcta cggtgcaata catcatggga cagcttgacg
     6841 aagatggatt caaaaaagct gtagaagact ggaaagcaca aggaggcgat cgaatcacac
     6901 aagagtacga ggaagcatac aaagcagcgg cacagtaata tagacgattg ggacaaatac
     6961 ttcttggtat ttgtcccaaa tcctatttta atttttaaag gagagcaaat aatggaaaac
     7021 tggtcaaaga aattagcaaa aagtatcatg gaaagaacac ctcgcttata tgaagaaaag
     7081 tggtataaag gaaaatggtc ttatgactat ggcgtcgtac ttaaagggtt ccaattactt
     7141 tgggaacgaa cccaagaaaa aatttatttt gatttcataa aagacaatat cgactatttc
     7201 gtgcaagaag atggaacgat ccgtggctac agcgttgaag aatacaatat cgaccatgta
     7261 aatacaggaa agcttttctt tttattgtac aaagaaacag gcgaagaaaa atataaaaaa
     7321 gcagcagaac tcctttccca gcagttggca aaccatccac gcacatccga aggagctttt
     7381 tggcataaag aaatttatcc ttatcaaatt tggttggatg gcttatatat gggttctcct
     7441 ttttatgctg aatttatcca gaattttagt gaagaaaaag attaccaaga tgttttgaga
     7501 cagtttgaaa tcagttataa acatctattc gatccaaaaa caaatttgct gattcatgca
     7561 tgggatgaaa agaaggtcca accatgggct gatccaaaaa caggtctatc ccatcatttt
     7621 tggagccgat cgattggctg gtatttgatg gctgcagcag atacctatga attacttcat
     7681 gaagaaacag attgttcttt gctgaaagaa attctagaaa agacactggc tgcattactt
     7741 ccatatcaag aagaaaacag cgggacatgg tatcaagtaa caacagaaac aaaacggaaa
     7801 ggaaattatc tggaagcttc cggcagtagt atgtttgttt acgcaatggc taaaggaata
     7861 cgtttgggac tgctgaacaa agaagaatgg cttccacaag tgaagaaatc acatcaagga
     7921 ttacttgatg aatttgttct ggaaacaaaa gaaggctggc tgaacttgaa taaaaactgt
     7981 caagtagcag ggttaggcgg agcagatcag cgagatggtt catatgctta ctacatcagt
     8041 gaaccaatta tttgtaatga tcaaaaaggc gttggtgcat tcttgcaagc attgattgaa
     8101 gtggaggcat tataaatatg tatgatattt tgacatttgg cgcatcaatt gatgaattga
     8161 atactgaagc aatacaacaa gccattgatg ctgcagcaag tgacggaggc gggaccgtag
     8221 ttgttccagc aggtgaattt ctaacaggag ctttattttt aaaaagcaat gtcgaacttc
     8281 atttaagcgc aggagcggtt ctgaaattct ctgacgatcc aaaagattat cctgttgttc
     8341 attcacgttg ggaaggagtc catcgaaaag tttatgcttc ctgtatctat gctcaaaatg
     8401 tagagaacat ctctgtcact ggtttcggta cattagatgg aaatggaaaa aaatggtggc
     8461 atacttttag aaatgaacca gataatctcg cttatcctcg tcctaaattg atgagtttcc
     8521 ataactgtca tcgaattact gtgaaagata ttaaattgat ccagtcacca agttggacga
     8581 tcaacccgat tctttgcagt aatgcaacgt ttgataactt gacgatcttg aacccagcgg
     8641 attcaccaaa tacagacgga attgatccag aatcatgcaa aaatgtgcgt attagtaatt
     8701 gccatattga cgtgggagat gactgtattg caatcaaggc agggactgaa gatacgtacg
     8761 aaaggatcgc ttgcgaaaat atcacgatca caaattgcac gatggtccat ggacatggcg
     8821 gtgtcgtatt aggaagtgaa atgagtggta gtattcgtaa tattaccatt tcaaattgta
     8881 tcttccaaga aactgatcga ggaatacgat tgaaatctag acgaggtcga ggcgggatcg
     8941 ttgaagatat tcgagtatcc aatatagtca tggataatgt gatgtgtcca tttatactga
     9001 atctttatta tttctgtggt ccgcgaggga aagaaccgta tgtatgggaa aaaaaggctt
     9061 atccgataga tgaacggaca ccagcattca gaaggatcca tttttcgaat atcacggcaa
     9121 ggaatgtcca tgcttctgca ggttttattt atggattagc ggaacaattc atacaagaaa
     9181 tcacatttga caatatcgat gtctcgatgg cccaagatgc agttcccggt gttcctgcta
     9241 tgatgacagg aatagaggaa atgagcagac aaggatttta tattggcttt gcgggtaagg
     9301 tttcctttaa ccagatgtcg attgaaaatc acgagggacc agcatttcta ttagaaaata
     9361 ctaaagaaat cagtattgag aaattccaat caaatcaatc agaaaatatg gatgaactgg
     9421 ttgttcgtaa aaactttgct tatgcagttg aatgattttt taggtgctcc atctagcaga
     9481 cataccccta gtggaaaacg attataccga gaaaaacaaa acggttttat gcttgaatac
     9541 tatcaattgt acttaaatga attggaaaca gtaccagccc tctatgctta tccagaaaaa
     9601 gaaggaccgt ttccgccggt cctttacctg cattctcacg gcggagattt ttcagtcggg
     9661 aaaagtgaat tgatccatgg tgctccttac ttagcttctc catcttttgc agaagtactg
     9721 acaggaatgg gatatgctgt ttggtcgatt gatgcttggg gatttgaaga acgcggaggt
     9781 atttctgaaa gcgagttgtt caaacaattt ttattaacag gaaaaacgtt atggggaatg
     9841 cgtatttatg atgtgatcag cttgatcgat tatttggaga caagggaaga cacagatact
     9901 caacggctcg caacgattgg tctttctatg ggaggcttat taagctggtg ggtgacagca
     9961 ttggattctc gtgtcaaagt ttgtattgat ctagcggcac aggtggacat cgaaatactt
    10021 ttgctacatc gccgcctaga tcatcatggg ttttattact atgtgccaaa tctattgaca
    10081 gcttatacaa cgttagcaat ccaagaaaaa atcgctccgc gccctcgtct tagcttagtt
    10141 ggaaaaaacg atacgatgtg cttggacgaa ggcgtactga agctgagaaa agggctggac
    10201 aaaaagtatc aatcaatggg tagtccagaa aacttttctt ctttttcttt aacaggcggt
    10261 catatggaaa cacaagaaat gagaaatatt tggaaacaat tcataaggga gcatttatga
    10321 ggatgaaaga caaactgata attggaaaga atgctataga taccccgacg gattttgaaa
    10381 ctatccaaga aggaatcgat tatttggcag gcctgccgga tagccatcca aaggtcttga
    10441 tagtaaaaga aggaagatat gatgaatata tagaaagcag attatccaat tttaaacttg
    10501 tgggagcagg aacagtagtt atctccggta atcgttttgc aaaacaggag tatcttgacg
    10561 gtagccagtt agggactttc cgaagtgcta cttttttctt agaaggatcg aatgtcgtat
    10621 tagaaaatct tcacatcgaa aatacagcag gtccaggaga aaaggtagga caggctgttg
    10681 ctttattcaa ttcaggagat cgcgtatctt taaaaaactg tcgactatct ggttatcagg
    10741 ataccttatg cacgggtccg ttgcctgatt tgcaaaaaga tggtactcct ttttgtacac
    10801 cggcttctgc caatcctgag tattgtcgtc agacctatga atattgctgg attgaaggaa
    10861 cggtggattt catatttggt ggggcagatg ccgtttttca tcagtgtcat atccatagct
    10921 tagccagtaa aaatcctgga tacatcacag cagcatctac gccgaaaaat caaaaaacag
    10981 ggtatcgttt tgaaagctgt accttgactg cagaacctga tactagaaac gtctatttag
    11041 gaagaccgtg gcgtccgtat gcaaatgtct tatttgaagc gtgtaagata ggtgggcatg
    11101 ttcacccaac tggttgggat aattgggatg acaagaaaaa tgaacagact gtcaaattct
    11161 ctgaacgtaa taacaattat gaaacatcga tcaaaagacc tgaatggata catttagaaa
    11221 agcagggggt tcagaaatga aaagaaaatg ggaactattg ctaggaatgg tcggtggcag
    11281 tctatctttg attttctttg gcgggttagc agttacttta agtaatatgt ctgcgtcaga
    11341 gtttaaaaaa agttatcagt cgcttgctgt agatcattca actttgtctt tagaaaatac
    11401 atttggatta ttacaggaca tgactggtct gtttgccgtt gtgttgttta tttcacttgc
    11461 ttttttagca gtagcattgt ttttgactgc taaaggaaaa tacttaacaa ctgccactgg
    11521 tttgtatttt ataacaggat tcatattgtt gattggcact cagtttattg cgtttccttt
    11581 tgcatttttt tatttcgcag ctggggcatt cagtttatat cgagtaagaa tgagaaaagg
    11641 agcctgaatc atgcagacta tcaaagtgaa aaatcctatt ttaagaggat tcaatccaga
    11701 tcctaatata ttaagagtga aagagcggta ttacattatt gtctcctctt ttgaatggct
    11761 tccaggaatc cgagtatatg agtcgaaaaa tttagtcaac tggtcacata caacggatat
    11821 tttgaccact caagtatctt tgcaaggaaa tcctacaaat ggaagtatat gggcgccaca
    11881 gatcagttat gctgatggat tgttttatct tgtttacaca gatgtcaaaa gtgcaggccg
    11941 tccgtttaaa gatgtccaca attatttgat cactgcatct gagatcacag gtccttggtc
    12001 cgaaccaatt tatttaaata gtaacggctt tgatccttct ttgttccatg atcttgaaac
    12061 gggaaaaaaa tggcttgtca atgaactatg ggattatcgt ctggacacac cgaataaatc
    12121 cagcggtatc gtattacaag aatatgatgc agagaagaag aagcttgatg gtcccatcta
    12181 taaaatattt gcaggtacag aattagcgaa aacggaggca ccacatttgt attttcgtaa
    12241 cgggtattat tatttgatga cggcagaagg cggtacagga agaggacatt cagttacagt
    12301 ttgccgagca aagaatatta ctggtcctta cgagcttgat cctggttttc ctatgctgac
    12361 agctagtgac aaacctgctt ctacattgca atgtacagga catgggagct tagtacaaac
    12421 cccgtcagga agatggtaca tgacatatct ttgtaccaga ccattaaacg gtgcggctat
    12481 tttgggaaga gaaaccgcta ttcaagaagt gtattggacg tccgacgatt ggttaagact
    12541 taaagatagc cagacaactc cgatggaaga aataatgatt gagacaaatg aagaggtaca
    12601 acaagaaaaa gatcatcagt ttatggatac atttgaagga gagctaaaga agaactggaa
    12661 tgccagacgc attttgccaa atgctgattg gtgtgataca aaatgtagac caggtttttt
    12721 acgtctgatt tccggtgaat ctcttcaatc taccttcgaa catcatttgt tagctattcg
    12781 gcaaacggat tttacttttg atgcggagac agcatttgaa tacatgccta ataattttaa
    12841 tcagatggct ggactggtac tgtacttaaa tgaatccaat tatatttatt gttatgttac
    12901 ttggaatgag gacaagggaa aatgcctacg tatgatagaa tcagaaaacg gagtagcaca
    12961 aatagaagat tggacgattc cactcacaga aaagaagact tttttgaaag tcactgtaaa
    13021 gaaagagaca gcacagtttc atttgaaaga cgtatcaact gaagaatgga agaaagtagg
    13081 tccgcaaaaa aacatgtgta tactctcagg agggtttact ggaaatttta tcgggatagc
    13141 agtgcatgat ctaaataaaa aaagaggaag ttatgctgat tttgagtttt tcaagtatga
    13201 tggaaaagat catctgtaat ttaattcttt aataaatgag gttcactaac ttattttcac
    13261 tagttagtga atttttttgt ttgtatatat tgttatgtaa aaaaagttat c
//
LOCUS       DAGCWU010000067        13148 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.067, whole genome shotgun sequence.
ACCESSION   DAGCWU010000067 DAGCWU010000000
VERSION     DAGCWU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.067"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(79..151)
                     /locus_tag="MBZ71_001524"
     tRNA            complement(79..151)
                     /locus_tag="MBZ71_001524"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
     gene            532..1101
                     /locus_tag="MBZ71_001525"
     CDS             532..1101
                     /locus_tag="MBZ71_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBT4429485.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1088..1948
                     /locus_tag="MBZ71_001526"
     CDS             1088..1948
                     /locus_tag="MBZ71_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4429486.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2138..2842
                     /locus_tag="MBZ71_001527"
     CDS             2138..2842
                     /locus_tag="MBZ71_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4429487.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2847..4682
                     /gene="walK"
                     /locus_tag="MBZ71_001528"
     CDS             2847..4682
                     /gene="walK"
                     /locus_tag="MBZ71_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBT4429488.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4679..5995
                     /locus_tag="MBZ71_001529"
     CDS             4679..5995
                     /locus_tag="MBZ71_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429489.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5996..6865
                     /locus_tag="MBZ71_001530"
     CDS             5996..6865
                     /locus_tag="MBZ71_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBT4429490.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6920..7729
                     /locus_tag="MBZ71_001531"
     CDS             6920..7729
                     /locus_tag="MBZ71_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBT4429491.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7832..8461)
                     /locus_tag="MBZ71_001532"
     CDS             complement(7832..8461)
                     /locus_tag="MBZ71_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBT4429492.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8631..9923
                     /locus_tag="MBZ71_001533"
     CDS             8631..9923
                     /locus_tag="MBZ71_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4429493.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9999..10661)
                     /locus_tag="MBZ71_001534"
     CDS             complement(9999..10661)
                     /locus_tag="MBZ71_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBT4429494.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10886..11170
                     /gene="groES"
                     /locus_tag="MBZ71_001535"
     CDS             10886..11170
                     /gene="groES"
                     /locus_tag="MBZ71_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBT4429495.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11221..12846
                     /gene="groL"
                     /locus_tag="MBZ71_001536"
     CDS             11221..12846
                     /gene="groL"
                     /locus_tag="MBZ71_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBT4429496.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4371 a         2265 c         2833 g         3679 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
      541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
      601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
      661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
      721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
      781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
      841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
      901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
      961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     1021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     1081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgtgccaga
     1141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     1201 ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt tgccagagga
     1261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     1321 aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata
     1381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     1441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     1501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     1561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     1621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     1681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     1741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     1801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     1861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     1921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     1981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     2041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     2101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     2161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgatgt
     2221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     2281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     2341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     2401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     2461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     2521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     2581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     2641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     2701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     2761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     2821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     2881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     2941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     3001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     3061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     3121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     3181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     3241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     3301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     3361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     3421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     3481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     3541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     3601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     3661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     3721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     3781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     3841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     3901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     3961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     4021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     4081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     4141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     4201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     4261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     4321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     4381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtggttttaa
     4441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     4501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     4561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     4621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     4681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     4741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     4801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     4861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     4921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     4981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     5041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     5101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     5161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     5221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     5281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta
     5341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     5401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     5461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     5521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     5581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     5641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     5701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     5761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     5821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     5881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     5941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     6001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     6061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     6121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     6181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     6241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     6301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     6361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     6421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     6481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     6541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     6601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     6661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     6721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     6781 taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga caaacaatac
     6841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     6901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     6961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
     7021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
     7081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
     7141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
     7201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
     7261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
     7321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
     7381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
     7441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
     7501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
     7561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
     7621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
     7681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
     7741 aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt
     7801 atttttttag catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct
     7861 tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga
     7921 tcgtactgtc gccagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg
     7981 tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt
     8041 tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg
     8101 catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag
     8161 aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg
     8221 acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa
     8281 catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa
     8341 catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca
     8401 atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca
     8461 taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga
     8521 atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag
     8581 ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt
     8641 ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg
     8701 ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct
     8761 tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggattttcta
     8821 tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg
     8881 ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag
     8941 ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga
     9001 caggtgctga agggatacta ggagcagtta caactgggct catcatcgga tggggctatg
     9061 cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt
     9121 ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt
     9181 cagcggtttt tcaacagttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc
     9241 agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg
     9301 ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc
     9361 ccgtcttgca agcgaattca ttagcgaatg cagaaatctt agcctcagga caagagttga
     9421 cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta
     9481 cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga
     9541 aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa
     9601 ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac
     9661 cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt cctttattta
     9721 cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag
     9781 tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat
     9841 ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag
     9901 cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa
     9961 tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca
    10021 gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag
    10081 atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc
    10141 agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa
    10201 aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg
    10261 atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg
    10321 atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca
    10381 gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt
    10441 ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa
    10501 aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca
    10561 gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca
    10621 tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg
    10681 gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat
    10741 aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag
    10801 aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa
    10861 aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga
    10921 agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga
    10981 gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga
    11041 aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac
    11101 agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat
    11161 tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc
    11221 atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat
    11281 aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa
    11341 aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa
    11401 ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca
    11461 aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc
    11521 gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa
    11581 ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa
    11641 gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt
    11701 gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg
    11761 atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa
    11821 tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc
    11881 acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa
    11941 caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt
    12001 gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc
    12061 gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact
    12121 gacgatctag gtttagaatt gaaagatgta acaatcgaaa acttaggaaa tgcttctaaa
    12181 gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc
    12241 gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc
    12301 gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt
    12361 gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct
    12421 acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt
    12481 atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt
    12541 gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca
    12601 gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga
    12661 gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc
    12721 ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat
    12781 aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg
    12841 atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac agataaaaag
    12901 aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga aattgctttt
    12961 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gctgtttata
    13021 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct tttttgtgtg
    13081 taattataat gaaaatagtt atttttaata gttataatat tttatctatt aaaagtaaat
    13141 atccattc
//
LOCUS       DAGCWU010000068        12974 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.068, whole genome shotgun sequence.
ACCESSION   DAGCWU010000068 DAGCWU010000000
VERSION     DAGCWU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.068"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(147..761)
                     /locus_tag="MBZ71_001537"
     CDS             complement(147..761)
                     /locus_tag="MBZ71_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4429497.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1367..1744)
                     /locus_tag="MBZ71_001538"
     CDS             complement(1367..1744)
                     /locus_tag="MBZ71_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429498.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2044..2925)
                     /gene="galU"
                     /locus_tag="MBZ71_001539"
     CDS             complement(2044..2925)
                     /gene="galU"
                     /locus_tag="MBZ71_001539"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBT4429499.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(2968..3987)
                     /locus_tag="MBZ71_001540"
     CDS             complement(2968..3987)
                     /locus_tag="MBZ71_001540"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBT4429500.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4620..5624)
                     /locus_tag="MBZ71_001541"
     CDS             complement(4620..5624)
                     /locus_tag="MBZ71_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4429501.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5637..6686)
                     /locus_tag="MBZ71_001542"
     CDS             complement(5637..6686)
                     /locus_tag="MBZ71_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4429502.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6703..7524)
                     /locus_tag="MBZ71_001543"
     CDS             complement(6703..7524)
                     /locus_tag="MBZ71_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4429503.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7511..8290)
                     /locus_tag="MBZ71_001544"
     CDS             complement(7511..8290)
                     /locus_tag="MBZ71_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4429504.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8307..8777)
                     /locus_tag="MBZ71_001545"
     CDS             complement(8307..8777)
                     /locus_tag="MBZ71_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4429505.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8789..9208)
                     /locus_tag="MBZ71_001546"
     CDS             complement(8789..9208)
                     /locus_tag="MBZ71_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBT4429506.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9289..12066)
                     /locus_tag="MBZ71_001547"
     CDS             complement(9289..12066)
                     /locus_tag="MBZ71_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4429507.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12257..12481
                     /locus_tag="MBZ71_001548"
     CDS             12257..12481
                     /locus_tag="MBZ71_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429508.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3802 a         2715 c         2118 g         4339 t
ORIGIN      
        1 aaatcaaaga tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct
       61 cattaataat cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt
      121 aaggaacttt gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt
      181 gctgtaaaat acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag
      241 atcgctggat ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc
      301 aaaaccaaat cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt
      361 cgtgacaaat caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga
      421 gctagttcga tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct
      481 gatttgacag aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca
      541 cctccggctg gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat
      601 tcaaatgcta caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta
      661 atttgtttcg cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc
      721 tcttccaatt ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct
      781 atacttgaca tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt
      841 tagacagtaa cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac
      901 gcccaatacc ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag
      961 gggtagaatt ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta
     1021 tggcaaaatg atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc
     1081 aactaagggt cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta
     1141 atctcttatc caagtgcaag caagaaaata caaaaaaaca ttggtatgat ttatatatat
     1201 ataaataaca taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag
     1261 attcttttat ataagtgaaa aaagcttttt tccaccaatt aaataaatct ctcatctcaa
     1321 tagtttatcc attattttat ctttttttgc gtatacctac taaatattac cgtcttcttc
     1381 tcgttcttct actttgttcg agagaatctg tactagaaaa agtagttgta tgtctacctt
     1441 ttctgcctat ccgagacttt cttttcatat atgggatata aatgattaga tacagtacag
     1501 caaaaaataa agcaaatacg caagcttccc aactacctaa cgccaattca accatcaagg
     1561 tagcaacccc aacggatgct gcataccaga taaaaatcag aaacgtttct ttttttctga
     1621 ccatgcgaaa catgataaat agactaataa gcaatagtgg caacacaaat atccaattgc
     1681 ttccttccat cagccacttg agtatatcaa gcagcgactg tgcaacagcg actaattttc
     1741 ccatagtgaa cacacacctt tttttataat taaaaaattc gcctttttat tctactatga
     1801 atcttctggc ttggctttca tttctcttat gtaatcattt tcttttatac aacttaaaaa
     1861 aaaattaaac cattagcata atattttctg aatctattca cgaaagtctt tttctatctt
     1921 tcgttttcca cgactcaact gatctagagt caaaaaacta aaacgacagt tgcgcaaatg
     1981 acgcacaagc tgccgcttta ataacaaata gcctttcatt ctcagcaggc cacttatttc
     2041 cagttaactt tctgttctta gctttttact caactggata atatatgcac ttaatgaagt
     2101 cccaatttcc gaatgttgaa gtccatattc aatatttgtc ttcatatatc ctaacttatc
     2161 gcctacgtcg tatctttttc ctttaaactc tctcgcatag accgcctctg tctggttaag
     2221 agtctgaagt gcatccgtta gctgtatctc gtttcctgct cctggttctt gagtttctaa
     2281 tatctcaaat attttaggag tcagtagata acgtccgata atggctaaat tacttggtgc
     2341 ttttcccatt tcaggttttt ctacaaaccc ttttactctg caacgaccct tttcgctatc
     2401 tgttttagga tcaataattc cgtatttaga tgtttcattt tctggtacac gcatgacggc
     2461 aacagcggaa gcttgtgttt cttcataatc catcatcaat tgttgagtca atgggatatc
     2521 atctttcatc aaatcatccc ccagcatgac gacaaacggt tcatttccta taaatgcttt
     2581 tgcttgaagc acggcatccc ccagtcctct tggatgagat tgacggatga agtgaagatt
     2641 gacttgagtc gtttcttcaa ctaaagcgag taattctgtt ttttcctttt cccgcaaatt
     2701 gttttccaat tcgatatttg catcaaagtg atcttcaatg ggtctttttt cttttcccgt
     2761 tacgatcaag atgtcttcga ttcccgaatc caatgcttct tccacaataa actggatcgt
     2821 tggtttatcc acgattggaa gcatttcttt tgccatggct tttgttgccg gcaaaaaacg
     2881 tgttcctagc ccagcggcag gaatgactgc ttttttgacc ttcacttgct gttcctccta
     2941 aacgacgatt aactattgat catctattta acgaagagaa aattcattct ccatttttcc
     3001 gtcacgtaac atgatttctt ttgctgcttt tttgatatct tctccattat acaaaacatt
     3061 atagattgtt tgagtaattg gcatctcaac acctagctgt tgagctagct ctactgctgc
     3121 ttttgtcgtt gccacaccct caacgaccat tcccatgttt tctagaactt cttccagctt
     3181 atgtccctgt cctaacaagt taccggcacg ccaattgcga gaatgaacac ttgtacaagt
     3241 aacgatcaag tcccccacac cactcaaacc gataaatgtc agaggattgg cacccatcgc
     3301 gacaccaaga cggctgattt ctgccaatcc tcgagtcatg attgctgctt ttgcatcatc
     3361 tccgaatcct aatccatgga tagcgccggc accaattgca atgatatttt tcagtgctgc
     3421 accagtttct acgccaatca catcattatt tgtataaatc ctgaaataat cattcatgaa
     3481 cagtttttgt acataggctg ctgctttttg atcaatactt gcagctgtaa ttgttgtgat
     3541 atcatgtact gccacttcct cagcatggct aggaccagat aatacaacaa ttgcctgtct
     3601 tttttcttct ggaatttctt cagccaaaac ttctgagatt cgtttatgcg tatcttgttc
     3661 tagtccttta cttgcatgta taatgattgg cttagtattc attttaccaa taagttcctg
     3721 tgcgactgaa cgaatcgctt ttgttggaac aacaaataaa atagcatctg cgttatccac
     3781 tgcttctgcc aattctttat atccgataat cgattctggg atcgtcaaat caggtaaaaa
     3841 atgctgattt gtatgatact tattgatttc atcaatctgt tcaggtacat ttccccaaat
     3901 acggacatca tgcccgtttt ctgccaatgt ttgtgctaaa gcagtgcccc aagatccagg
     3961 accaagaact gcgatttttt gtttcatgga tattcctcca aataaataat tgataatttc
     4021 cattatagta tacatttctt aaagttggga ctaatttcca ttgtttttaa gtgtttgttt
     4081 acttctttta tcgtttttac ttgaaataat caatgatatg ttacaatagt ctacatttta
     4141 tttttacact cgttatgctt tgcttgttcc taagaggaag cagttttgac aatgttacta
     4201 ccagcattac tattaggagg ttaggaaact tctcctagtg agtattgatt taaaatacat
     4261 aagagacaga ttgaacattt ctaaatctgt caggtcctgc aacggacaga ttgtctctgt
     4321 tgacggttga cataggaact tttgttttag tcaacattct ggatacaaaa cgctatgtaa
     4381 actggcgcaa ctcgttgtac ttggactttt actacggatc catgcaacaa ctaaatagta
     4441 ctattgcata acaaaaaatg gttggcaccc acatgtccca ttaaacagat tataagttga
     4501 actgtagata atgcaaaaaa tgtgttttat aacgtaattg atgccaatat tttaaaaaaa
     4561 taaagggagc agttatcgct gctctcttta ttttttatat tctttttatc cagtctatcc
     4621 taataatcaa actgacgata gtagcggcgt atctctaatg gatggcagtt caactgctcc
     4681 acatgagcat cgatgcgttg tgtcactaca tgcatcaata agaaagacaa aacacgtccg
     4741 cgatattttt cactgatacc ttctactgga tagtcttttg tgtctatcaa tgtataattg
     4801 ctgcaaattt ttggcaaaag tttagccaca cgttcagata acactctctg ttcatcttcg
     4861 cctaaaaata atgtgactgg ggttgtttct tctacgatct ctaacgtccc gtgaagaaac
     4921 tcggcagcat ggatagattt cgatggaagc cagctttgtt cttcccagta acacatggca
     4981 tatgaataaa cagctcccca ttgattccct gctccaataa aataatgcat actatcacgt
     5041 cgatgttttt ctgcaaatga taagccaaat gcatctgctt ttttctctgc ttccactaac
     5101 cctgttggta aataacgttc gagctgttca tagtattctg aataagcttc gaattctcca
     5161 ttcaaataca tcaatcgatc tgctgccata aaaaatttga tttgttctgt tccaggagct
     5221 ggataagtga ccacaaaatc gcatagctga gacaacttac tgttcgcttt atcgataaat
     5281 ccaatcagtg tcgctcctac tttcttgatt tgttctactg ctttcactac ttcttgagtt
     5341 gtaccggtaa cagaggacaa aataacgata gaatcttttg ttaatctttt gtttccagta
     5401 gtgtaatatt ctgcagcatg ctgtacaaag acaggcaagt tgcttttccc attcatataa
     5461 gtgacagctt gcatcgagga agcatacgtt ccaccgattc ctagataata gacagcgtca
     5521 aatttttccg cataaagttt atcgatgatt tcttctactt gcggacgtaa agctagtgcg
     5581 ccttttattg ctttgatttg ttcttcttca ttgaatttca gcatgatttc cacctcttat
     5641 aatttacttt tcaagactgt atcaaagtct tcaaaaatct ttttgttcaa gtcaatacct
     5701 ttagctactg cggttttata agcgaatagt tgaaacggaa cgacaagcgc taatacagac
     5761 agatattcat cacattctat tgctaatcca agtgttttct cgtttcttgc tttttttgtg
     5821 gtaatggtga gagtctctcc aacgtaccga ctcatatatc tttgcaactt ttgcgaacgt
     5881 tccttattta cactttcccc ttcaataaag aatagcaaat gcgaagcatc tgcctccagg
     5941 tatgggccgt gcatataagc ttctaactca aatccttgtg acggaacgcg gattgtttca
     6001 gtcaatttcg tctctgcttc tttagctgtt ccccagtttg gaccatatcc aatggtgacg
     6061 aaacgctgtg ctaaacgaaa taaactttga tatccatcaa aaaattcttc cgttttttga
     6121 ataatcgctg gtaaatggtt gattattttg cgcaactgtt gcatgtagtc ttgttctatt
     6181 tttttgctga ttttattttt acttattcca attcctatcc ctaagagtag taactgtaaa
     6241 acagtcgcag aatagccttt tgtaacgaag cctactgatt ctaccccggc acctaaatca
     6301 ataatttgtg cagcttctaa agcaagtgga ctttgcacgt catttgtgat agcaacaaca
     6361 ggtaaccctt gtttttgtat catttttact gcttcaacag tagaagcact tttcccgctt
     6421 tgtgacacag caatcacagt atcgattgaa ggagaaagtt ttccataatt gaaaaaataa
     6481 aaaggttctt gaatatcgac agtaagatca ccataagatt ccaatgctgg cttcgctgct
     6541 aaacaagcgt tataagatga acccgttgct aagatcagta aattcactgt ttttttcata
     6601 tgttctcttg tcgaaaaatc attctttttc aagatcgtac ttagtacttc cttttcttca
     6661 tagatataat cttgcatcgt aaacattttc tctgctcctt ttttaaaaaa tccctatcca
     6721 cgaaccaaaa attccaaaga aagctagtcc gatcaagatc gttaatggtt tcacttcttt
     6781 cttcaataga taaaacacta tgccaaatgc tccaagacat aagatatttg gaacgatatc
     6841 atcaaaaata ctttgaacag tcacagcatt ttctcctgaa ccgattttta aaggaatatt
     6901 gatggtgatc atcgtggcag tcattgctcc aacgaccatc agtccaataa tagacgcgcc
     6961 taatgtcaaa ctttccatca taccgtttgc ctggatcttt ttcaaaaaac ccgttcctaa
     7021 gcgatatccc caacctgttg ctagatagcg aaacagaatg tgcggaatat tgaaaatcaa
     7081 aatgaacaag atagggccta aaatatttcc ttgcatggct aaagaggtac ccacacctgt
     7141 ggcaatcaat cgcaaagttc cccaaaagaa agaatctcct aaccctgcaa gaggtcccat
     7201 caaagatgtt ttgatactat cgatcgcagt cacatcaaat tcaggatcat ccttattttc
     7261 ttcctccatt gcagccgtga ttcctaaaat cagtgtaacg atatgtgggg tcgtgttaaa
     7321 aaactctaga tgccgtttca aagctgctgc catttcttct ttcgactgat ataacttttt
     7381 caaaatcgga atcattgcat aaacataagc catattcatc tgacgttcat aattccaaga
     7441 aaactccatt tgaaaagaac gccaaaatac gtgatttagc tcttttttcg ttattttgct
     7501 gtttttgatc tcagaattct tcatcttctt ctacctcccc tgcagttgtt tgaagtggta
     7561 gttgacggac attcataata ttgactacca caactgctaa aatcgcacca aaaatagcta
     7621 ctccggtcac aggaatctct aagtaagaag ctatagcaaa acctaagaaa aaatatggtg
     7681 caacttgttt gttgatcagc aaacgagcaa gcatcgcaaa accaagagct ggaatcaacc
     7741 ctgtagctac tgacaacccc ttttgaacga agtcaggaat catatttaat agatttccga
     7801 tcgtattgct tcccaccata taagaaacca ttaccacaac accaagcatc aaggaaagac
     7861 cgataccggc aagcaagtgc atatgctcga ctcctttata atttccttct tctgcataat
     7921 tgtctgcttt atggttcata attggaatca aaagccctaa gtaaatattt ttcaatatca
     7981 atgtcaacgt agcaatcggc aaacctagta aaagcgcggt ttctgtaccg gctccagctg
     8041 taatagcaaa agccgtccct aaaatcccac cggtcacaac atcaggtgga atcgaagcac
     8101 ctactgagaa cgatccgata aatgctaact ctaaagttgc gcccataatg atcccagttt
     8161 ttacatcacc taacacgatt cctgtaaaca gccccgtcac aatcggacga gaaagcaacg
     8221 atgtccctag tgcatattct gattgtgcga caaatgcaac aattcctaat aagactgctt
     8281 gtacaatcat tgaggttctt cctttcttac aaaacttctc ttacatccac ttttttatct
     8341 gctggtactt gacggatttc tacttctatt tgtttgtcaa ttaatcgttt caagcgttcc
     8401 tcctcttctg gtaaaatatt taccgctttt ccgatgctgc gagtaccagg ttttgccttg
     8461 atccctccta agttgatggt tttgatttct ggatacgctt ctgccagctt atatgcatcc
     8521 tcgatcgatt ccactacgat gaataactta tatttatctg tcaccccgct tttcaacgcg
     8581 gcaatcgagt catcaattga tttgatcact agtttgactc cttgtggctt ggctaatttg
     8641 atcgttgtct ttcgtatctc attcgtaggt acatcatcat tagcaatcaa aatacagtct
     8701 gcgcctattg tttgtgtcca tgaaaaagct acttgtccgt gtaataaccg atggtcgact
     8761 cttgtcaata caatcatttt tcttcctcct aaaattcgtc ttcttctatt ttctgattga
     8821 gccgctcgtt acaaaactgg atcatttttt ttgaatcagt tagcacctgc tggatcaaaa
     8881 cagttgtctc tacttcagcg tccaaccgag atactagctc aatcaatagt ggcaaattca
     8941 atccggcaac gagatagaca tgatcccgat tgatatattg taagaattcg ttgttgacac
     9001 ttccgccaaa aagatccgtc agaacaatga tttctttatc tttgtacctc ataaaaagct
     9061 gccttactgt ttccgctaaa tcaaaattct catcagtata gcaatcgaat acagtaatag
     9121 aatgattttt cccatagatc agctctaatg aatctaatat ccctgaagca aattttccat
     9181 gggaagctaa gataatttct ctatccactt tttcatctcc tcttttcact tacacatact
     9241 aaaaaagcaa gttgcatgcc aaaattagtg gcaacaactt gcttatgttc aaaattcttc
     9301 ttctatcgag agaaactctg tatcttcact caaaatatca tagatatagg caatctcaga
     9361 attggggatc atgacactat aatgctcttc aatgacacta aacgcagatt taatagcaga
     9421 taacccttct ttttgacact gcttccattt ctctaatcct tgataggttt cgattggcat
     9481 ttgtcggatc aatcgttcaa tgagacagct cacatggaca tacaacgcta attttttggg
     9541 attgctgatg cttcgattca actgcacctc taattctctc ataaaatgat cgatttctcc
     9601 catcaccttc tctgtatcta gaatagtcac ggaatccagt actttttcca atgaaaaatt
     9661 acgaatgatg cgctgattga atatttcctt ctctgaagca ttcatagtag tgtccaacca
     9721 ttcagctagt acagacatct tttcccccga gatgaggtct tctaatgaaa gatagtcgat
     9781 attggtgata tcaggatctg tggtgccaat cattcctacg acttcgtata tggaaaataa
     9841 tgggtcactg tttttcttat cttttaattg ctgatattca tatgggatga ttttcaactg
     9901 acaagccgta ggtaagcttt tttctagcag ttcacttaag tggaccgccg taccgattcc
     9961 agtgaagcaa gtagtaatga gtgctttggg tttgttttcc tctggataga gcagtcgcca
    10021 ttctggctgg atctgtcgaa cagtctcttc tgctatctct tctaatgata catttttttg
    10081 caggcattcc cctactgtga ttgctaaagg tgtcgtcaca ttattcaaaa tcaaaagcgg
    10141 tacggataac tgttttttaa aaaactgatg gatttctttc agagatccca tgtctactaa
    10201 tatgatcaat ccatttgaaa catcgctgtt ttcgcaataa tcaaggattt ctgaagcaat
    10261 ctgctgtggt gtgacatcta gtggcatatc aaatgattca aatagatcct cttgtaataa
    10321 acgatttact acatttgcca tactgctagc agttgcataa ccgtgagcag caatcatcgc
    10381 ttttggcagt cgtttttctt ttgaccattt cgcttttttt agatagatcg tcagtaaaat
    10441 ccgatccata cttgtgtctt caatatctaa tttgatctta cttaattcca gaattcgttg
    10501 gacatagtga taactagatg gataagactg agaaacttgt tgttctaatt ctttgattag
    10561 ttggataata tcatgttctt ccggtaacca tttgatagtg cttcgctgat agagatagta
    10621 actcaaggca tagacactgt ttccatcgaa tttgatttga taggcagttt ccatttgtcg
    10681 aaaggtctct cggatagatt gtgtccaata aaccagcagc tcgtgttttt cttgtctact
    10741 ggtctcaaac agcaaaaaat caaataattg aatcacttct tgctttaatt ttccttcgca
    10801 agaatatagc agacagttat ttttcttgaa atcaattagg atttgctcaa aacttttaac
    10861 gattcgctgc tgttcgggct gttgcttctc cattaattgc tctaaggtct ggtcttgcgt
    10921 aatatagatt ccttgctgtg ctgtattttt agtaataggc tgcgcaagta gttctttttg
    10981 caaatgatgg atcgtgacat ggatttcagg ctgttcacgc tgttcagcat atgcttttgc
    11041 tgctgaaact ttgacgatat ttttcagctc tcctatgttt ccggataatt tctgacttgt
    11101 taaaagtgac aaagctggtc cactgactga caaagggctc cccatttttc tttgttcttc
    11161 gagaaaaaaa gagtaaatca gttctagccg ttcattacga ctacgctgat taagtgcagg
    11221 tagttcgatt cggataggta ttcttcgaat gaaagttgtt aagaaatgac tagtaatctc
    11281 ttcagtcgta gcaaaaaaga gacgtgtttt gatccggcgt gacaaattag gttctcccat
    11341 acgataaatc acaccttgat caagataggt gaacagtttt tcctgacctt ctggtgttaa
    11401 gcgatgtacc tcgtctaaaa acaagatccc accatctgct gcttcaaaag cccctttttt
    11461 attttcttct gcaccagtgt acgctccctt aacatgtcca aacaaattgc tagtcaaaag
    11521 ttcaggatta tctgcatatt gtgcacaatt aaaagtgatg aatggtgcag aatcttctag
    11581 cagatcatga agcagacaat actgatagac caaattaact aaataacttt tacctgtgcc
    11641 actttcgcct gtaatcaaaa gtggtaatcc gccatctgga tagtaaagag ctgttttgat
    11701 ttgttcgata ctttctttca aactggcgtc gtatccgatc ataaaagaga ataagtcttt
    11761 tggttgcgcg aaaaaaggct gttcctcttt gagatcatcg atagaaaaat aaaaattcgt
    11821 gcgtaactta aaaaattgtt tttcaaaagc ttctttgtgg aaataaatca ctggtcgaga
    11881 attgattttg actagtagtt gctcatcatt taactggttc aaataatgac tgactgtgtt
    11941 tctttttacc ccaaaatgat cagctaacct ttgtgctgta aagacctcgt tcatatgttc
    12001 taaatcgaaa aaggctgtct ggttctctaa gtaacataaa atttccttct tcaacaactt
    12061 attcacccct cctccatcat agaaaacgga ttcattttgt caataaaatc tttaataaag
    12121 aattgaaaaa tatatcttat tatagacagc agcctactta tcccttataa tttttattct
    12181 aaaaaacgta cagttccttc cagtttttat actggaattt caacatatga aagaataagg
    12241 aggctatgac tattcaatga aatcggataa atataaaaac aggaaacata cttcctggaa
    12301 aacttttcga gaaatcatcg gatcttatct cgctgtagtt gtatttttgc ctttgcttta
    12361 tatggctatg acagaacaaa ctcgttttat tggcgtagat acgcaatata tagccgaaac
    12421 aggattttta ttcatcttta tcttgatttt tgcgttctat aaatccttcc atacaaaata
    12481 agattcacag atttcaactt tcctgtctgt ttttagctat tacaaatcct tatatgtggt
    12541 ataacaacaa tttattggca taatgaggaa aaacgagtta tgatgctttc agagagtaaa
    12601 gaggtactgt tgtcaccatc ctttttattg attacgtaca ccactcttag ttttttccca
    12661 ttgattacga aaacaaggta gaaacattga tgttcctacc ttgttttctt tttttatttt
    12721 cagatcaaaa tctcaagtag cttttgactt gatgtcggga ggttgtcttc gttccatcaa
    12781 acctgataga atctgcgcca aaccatagtt ttctccacta ttatttattc ctctaagata
    12841 gtacccacaa taaacgaata aatatttttc tcattttcat actccttctt tgaaaatcaa
    12901 ttagatttct ctcaacccac ttttttacaa aaattacgta gcaaaaaaga ttgcaagaac
    12961 aaattaaaaa agaa
//
LOCUS       DAGCWU010000069        12905 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.069, whole genome shotgun sequence.
ACCESSION   DAGCWU010000069 DAGCWU010000000
VERSION     DAGCWU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.069"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            73..975
                     /locus_tag="MBZ71_001549"
     CDS             73..975
                     /locus_tag="MBZ71_001549"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBT4429509.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            1188..2963
                     /locus_tag="MBZ71_001550"
     CDS             1188..2963
                     /locus_tag="MBZ71_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBT4429510.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3029..4615)
                     /locus_tag="MBZ71_001551"
     CDS             complement(3029..4615)
                     /locus_tag="MBZ71_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBT4429511.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFILLLLTKVGFRKIESLVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            4865..5089
                     /locus_tag="MBZ71_001552"
     CDS             4865..5089
                     /locus_tag="MBZ71_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBT4429512.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            5231..6982
                     /locus_tag="MBZ71_001553"
     CDS             5231..6982
                     /locus_tag="MBZ71_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4429513.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            6982..8766
                     /locus_tag="MBZ71_001554"
     CDS             6982..8766
                     /locus_tag="MBZ71_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429514.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            9092..9943
                     /locus_tag="MBZ71_001555"
     CDS             9092..9943
                     /locus_tag="MBZ71_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4429515.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            9997..11748
                     /locus_tag="MBZ71_001556"
     CDS             9997..11748
                     /locus_tag="MBZ71_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4429516.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            11752..11922
                     /locus_tag="MBZ71_001557"
     CDS             11752..11922
                     /locus_tag="MBZ71_001557"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4429517.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(11962..12864)
                     /locus_tag="MBZ71_001558"
     CDS             complement(11962..12864)
                     /locus_tag="MBZ71_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4429518.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT         4111 a         2304 c         2685 g         3805 t
ORIGIN      
        1 tttctttgta tatcatctta ccaatatata ggaattgtta gtaacaattg cctttcaagg
       61 aggaatccat ccatgttatt aggctcacac gtcagcatga gcggaaagaa aatgctgtta
      121 ggagcagcag aagaagcagc cagttatgga tcttcgactt ttatgatcta tacaggcgcc
      181 cctcaaaaca ctagaagaaa agccatcgaa gagatgaata ttgatgccgg aaaagcgtac
      241 atggcagaaa aaggtctaac aaatatcgtc gttcatgccc cttatatcat taatttgggg
      301 aatacgatca aaccagaaat gtttcctttt gccgtccaat ttctgagaga agagattgtt
      361 cgtgcggaag ctttaggcgc tactcaaatt acacttcacc caggtgctca tgtaggggca
      421 ggaacagaag ccggaattgc tagaattatt gaaggcttga atgaagtttt gacaaaagaa
      481 cagacggcac aaatcgcttt ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca
      541 tttgaggaat tagcgcaaat catcgatgga gtaaaattga atgaaaaatt atccgttact
      601 tttgacacct gtcatacgaa tgatgccggt tataatatta aagaagactt ttcaggtgta
      661 atggaagagt ttgataagat catcgggtta gatcggctta aagtactcca tatcaatgat
      721 tctaaaaatc cgcaaggttc gcataaagac cggcacgcca atattggttt tggaacaatc
      781 ggattcgaca cgctgaataa gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc
      841 ttggaaactc cctatgtagg caaagataaa aagaaagctt acgcaccata tggaaaagaa
      901 atcgccatgt tcaaagcgca aacattcgat ccagaagtgt ttcctgagtt attagcagaa
      961 gaagtgatac tctaagattt tctatctgac agcagcaaaa aaggaccagg cacattgcct
     1021 tgtccttttt tgtttaagaa acaaaggagt actttttgca gtctttttca tcatgcatct
     1081 tctcccccct ctttttaaat ttagacaaac aaggtatact ggtagaaaag taaaaaaacg
     1141 aaaagtttca caagcaagtg gcaaaacgtg attggaagga cgaaaagatg ctgaacactt
     1201 taaaaccctt gatcaaccaa ctaaaaaaga aacatacata ctttattctt ggcatagccc
     1261 tattatttac aattattcgt ttgtatttat atagtaaagc aatctatgga acagacatgt
     1321 cggcaaccta tgatgaccag ctgctgattc gctatgctca aaatattttg gacggtaaat
     1381 ggctagggga gtatacgact acaacacttt ccaaaggaat ctcctacagc ttatttttag
     1441 tacttgcaca taaactgcat ctttcttatg cagttttgtt cggcatgttc agtatttttg
     1501 ccagtatatt ggttgcactc gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa
     1561 gtatctattt ttattttttg ttttctccga ttagttttac acatgaatat tccactcgaa
     1621 catatcgtaa tgccctcgtg attccggctg ttgagatcat tatcgcttgt ttattagcta
     1681 tttattaccg caaatacggt tctagcagaa aattgctgcc atggttttta gggctttctg
     1741 tcgcttttcc tttcttttgg ttcattcgcg aagattctat ctggcttctt ccttttactg
     1801 tagtagcttt gggtatcagt ttcgtccaga tcttgctagg gcaagacttt tctttcaaag
     1861 aagtaaagaa gttaaaaaaa cgactctttc ttctctcgaa acgggaatgg ctgaaacttt
     1921 cattgttttt acttcctata gttggctttt ccatcgtcta tcaatggatc gaatataaaa
     1981 atgagacaac atacggtatt gcaaccatca atgaccgttc cagtggagaa tttggacgtt
     2041 tgatgaaaca actgatccga atagatgatg aaacgaactt gaataaagaa aacagtgaga
     2101 tatgggtctc acatgctgca ctaaataaag cattagccgc atctcctaca ttccataccg
     2161 tttctacccg tattgacgaa ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa
     2221 aagaattacc cggagatatc atcttttggg cattgcgaga tgtggtaagc gaatctggtt
     2281 attaccaaaa caatgcaaaa gaaaccaatg atttttggaa aaaagtcaac caagaattag
     2341 aagaagccta tcacaaggga acaatcaaaa agaaaaaaga gttctatctg atgggatcag
     2401 gagatggaaa gacgatgagt gatctgccaa tcgtattcga tttccttcaa tcaggatacg
     2461 cttatgcatg gcgatataaa gatttccatc aaggaggaaa ttatagtttc ggaacgcaag
     2521 aacaagtcaa acaggctcaa gaattattaa accttccgct aatggagaat tggatcgata
     2581 gtaatgaacc gagaacacct aatttccaat taacaaaatc agcaaaagtc gcgaatgtca
     2641 tcataaaaat ttaccaagtt ctggctattc ccgtgctgat tgcttcaata gcagggatat
     2701 tacttatgat actaggaacc gtatttgcaa aagctgatcg tgcctattat agttcctctt
     2761 tgattgtggt caccggatta atactgacac aattggtttt tcttttcggt gtctcatggt
     2821 tttgttctta tgcgccagaa aaaaaggact attttttaag tgtctacact ggtgcaggcg
     2881 tgccattaac gcaattcatc gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga
     2941 aaaaagcttt atcttctaaa taaaaagacc cttggtagct agatttttaa cctaactatc
     3001 aagggtcttt gtgtggaaaa atcagttttt attcgatttc ttctaccgtt tcctgcaaaa
     3061 gctgttcata ttttttgttt cctttatata aatcaccaat cgtccatgcc aacaggagaa
     3121 ggacaagaac aatgattaca taagcgatgt gatcagccaa tgctagttga gcggaagtgg
     3181 cttgctcgcc gaagaaactt tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta
     3241 gactaatcac agagaaccag ccaagaagtt ttacgatgaa tgaattttta aatcggctac
     3301 ccatctccgc ttggctgttg gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa
     3361 aagccaagaa cacctgggat tcattcatta agttattcaa agcggcatgt tcttcgatcg
     3421 ttccttttgt acttgtaaac aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg
     3481 tgaccagtct tcttagccaa atcgggatac gcatatggat gaatccttcc atgatgactt
     3541 gaccggtcaa cgtaccagta atagtagagt tctggccaga agcaagaagt gctacagcaa
     3601 ataaagtgga caagatacca gattttgcta cagcaatcaa aataccattg ctcatagctg
     3661 atggatcaga taaagcttca tacagaccaa aaaaggaagg atcttggaca gaaccggttt
     3721 tgaagacagc gacacccatg atcaatagca gtgcattgac tagaaaagcc attgacaact
     3781 ggatattaga atcccaagta gaaaaacgta cagcttgtgc tacatcttct tcgttttggt
     3841 gatcgatctt ccgagtttgt gaaactgctg aatgaagata taagttatgc ggcatgactg
     3901 ttgcaccaat aatccctaaa gctccagtca gcggagtttc gccgccgatg accggtgagt
     3961 ctgagaatgt atgactgtta gggataaagc cagcaaataa actgtgccaa tctgggttag
     4021 acaagccaac ttgatagaca aaaacgaata aaatgacgaa aattaagcag acaaccagtg
     4081 attcgatctt acgaaacccg acttttgtca gtagtaagag aatgaaaaca tctaatacag
     4141 taataaagac ggcaatcacc aatggaatat caaaaagtaa gtagagggcg attgctgcac
     4201 caatgacttc tgcaatatct gttgccataa tggctaattc agtcaagatc cataaaatga
     4261 cacctaatgt cttacttgtg cgtgctcgga ttgcctgtgc caaatccatt tggctcacga
     4321 ttcctaattt tgctgccata tactgtaaaa gcatagcaat cagactagac atcaaaataa
     4381 ctgacatcaa taaatattgg aaattttgtc ctccggtgat cgaagtagac cagttacccg
     4441 ggtccatata cccaactgct actaacgcac caggtccaga gtaagcaaga agtgttttaa
     4501 aaaaactttt cccctttggt acatcaactg ttccatttac ttcagctaaa gataaaccat
     4561 ttgtatgttc aataagtcga tgcttatgaa attgtttatt ggattgatta ttcaagaaaa
     4621 aaccaccttt cctatttatc tacgattaca ttctacacct caaaaagaaa aaattaaaga
     4681 aaaagattcg gtacgcctaa tttttttcat ggcgcgtctt gctagatgac gaaagaaatt
     4741 ttgttctcct ctaaaaaaac cactattttt tgcatgaaac taaagaatac gcctttcttt
     4801 tttcaataaa ctttagtaaa ataagtcatt gagagcaatt cggaaataga aaggagagat
     4861 ttatatgtta tcaacaggta ttgtcgtact tattgctatt attgctttgc taatcggcgc
     4921 agtaggtggc tttttcctag cgcgtaaata tatgaaagac tatttagaaa aaaatcctcc
     4981 cgttaatgag gacatgttgc gatcaatgat gatgtcaatg gggcaaaaac catctgagaa
     5041 gaaaattcgc cagatgatgc agcaaatgaa gaaccaaggg aaaaaatagg ttctgaccca
     5101 acgacttttt acgggctgat tttttcagtc cgttttttat ttcgtaattt gttagactat
     5161 tcagattatt cagcaaaaaa gacaagcaca gacaatccta ggctcttgat aaagacaaag
     5221 gaggagtaaa atgtcgattt ttagaaaatt aggctggttt tttcgtcagg aaaagaaaca
     5281 ttatttaatt ggggttttct cgctattcgc agtggccttg gtacagttag ttcctccaaa
     5341 agtgattggg atcatcattg atgagatcgc cgataaaaat ttatcaataa agatcatttt
     5401 cgggtggatc ttcctattga ttatcgctgc ttttttacaa tatctttttc gttatatctg
     5461 gcggacgaat atttggggaa gtgcagctcg attggagaaa gaacttcggc agcagttatt
     5521 caaacatttt acagaaatgg acaatctctt ttaccaaaaa tatcggactg gggatttgat
     5581 ggctcacgca acgaatgatt tgaatgcgat ccagaatgta gcaggagctg gtatcttgac
     5641 ctttgcagat tcagtgatta cgggcggaac aacgatcatt gcgatgattt tgtttattga
     5701 ttggcgctta acactgattg cgctgattcc tctgcctttg ctggccgtca cttcacgagt
     5761 gctggggtca aaactccatg atgcttttcg tgattcgcaa gaagcttttt ctagtatcaa
     5821 tgataagaca caagaaagta tcactgggat caaagtcatt aaaacatttg gacaagaaaa
     5881 agaagatcta gcggattttg aagaaaagat tgaagatgca attattaaaa acaaacgagt
     5941 caatttttta gactccttat ttgatccatt cattaccttg attatcggtc tgtcttatgt
     6001 tgcaacaatt attttaggtg gaaagtatgt cttggaaggc acgatctcga tcgggcagat
     6061 ggtctcattt attagttata tcggtatgct tgtttggccg atgtttgcga ttggtcgatt
     6121 gttcaacgta ttggaaagag gaaatgcttc ttatgatcgc gtcaatgaac ttttgcatga
     6181 aaagacgcat atcgtggaac aactgggtgc agtgcagacc ccggcaaaag gcgagctgaa
     6241 tgtagctatt tcctctttta attatccgaa agaagaatca ccagcattag aacaaatcaa
     6301 gtttgcgatc caagaaggag aaacattagg tattgttggg aaaactggtg cgggaaaaac
     6361 gacgatcatc aaattactga tgcgtgaata cgatgattat caagggaaaa tccaatttgg
     6421 cggacgttcg atcaaagaat attcattgaa tgctttatta ggatcaatcg gctatgtgcc
     6481 tcaggatcac ttcttgtttt cgatgaccat tcgagataat atccgttttt ctaatccaaa
     6541 actttcagaa gaagaagtag agcatgcggc ttctttagca tttatcaaca atgaaatcaa
     6601 ggaattacca aaaggttatg ataccttagt tggagaacgt ggagtctctt tatctggcgg
     6661 acagaaacaa aggatttcga ttgctcgagc attgatcaaa aatcctgaat tattaatatt
     6721 agatgatgca ctctctgcag tagatgccaa aacagaagaa gctatcttgc ataatttaaa
     6781 agaaataaga aaagagaaaa caactatcat tgcagctcac cgtttaagta gcgtgatgca
     6841 tgcaaaagaa atcattgttg tcgatcaagg gaaaatcatt gaacgaggaa cccatcatga
     6901 gctgcttgca ctaggcggct ggtatgaaca aatgtgggag aaacagcaat tagaagcgaa
     6961 gatagaagga ggcgaggcct gatggatgaa aattatcaat cggaatggac aaaatcgatt
     7021 ccacttaaag aacagaaaaa gatcatcaaa cgactgatga agttcgccaa accttttaga
     7081 agaacgttca tcgttgctat cttatttgcg tttgctttgt ctgtgatcaa tgtcctattg
     7141 ccaaggatca tccaaacttt tatggatgat catctggcta aacagtcggc tacgacacaa
     7201 gttattttgt tttttgctgg tatgtactta tttggagtca tagtaaaaag tatcatttgg
     7261 tttttccaat ggtatttgta ttccatggcc tcattgaaga cctatcagta tattagggta
     7321 aaattatttg aaaagctgca tacactaggt atgcgttatt ttgatcagac accggcagga
     7381 tcaaccgttt ctcgagtgac gaatgacaca gaaacgctat ttgaattttg gtacgtcttt
     7441 ttgatggtat tgactgggat ctttgcagtc gtttcttcat tccttgccat gtttcaaatc
     7501 aatggagaaa ttgccttgtg gactttggtg tttttgccga ttttaggaat cgtcatctgg
     7561 tattaccaga aattcagttc tcgtatttac cgaaatatgc gggaaaaatt aagtcagctg
     7621 aacacgaaat tgaatgaatc gatttccggc atgcgtatta tccagcagtt tcgccaagaa
     7681 gctcgtttag ctaaagaatt tgaacaagtg aacaatgagt atttagacac gcgttatgcg
     7741 atgatcaaaa cgaattcgat tctgttaagt ccgatcatca attttctata tgcattggcg
     7801 attgcgttgg tgttgacttt gtttgggatc gatgcgttgc attctcccgt agaagttgga
     7861 ctgatctatg catttacgac ctatgtccag ggatttttta atccaatgtc tcaaatgatg
     7921 gactttttga gcatcttcac agatgggatg gttgctggta gtcgtatctt gaagattatg
     7981 gatacggaag aatatgcacc acaacaaaac cctggagcag atcaaatgat tacttcaggt
     8041 aagattgagt ttcgcaatgt aagcttttct tatgatggag agcacaatgt attaaacaat
     8101 atctcttttg ttgctaatcc gggagagacc gttgcattag tgggacacac cggaagcgga
     8161 aaaagctcga tcatcaacgt attgatgcgg ttttacgaat tttatgaagg tcagatattg
     8221 attgatgatc atgacattcg agaatatcca atggaagagt tacgtaagaa aatggggctg
     8281 gtcttgcaag atgctttctt gttttatggg gatatcgcag gaaatattcg gatgatgaat
     8341 ccagctatca cagacgaaca gatacgccat gcggctgagt ttgttcaagc aaacaaattc
     8401 atcgatgaat taccaaaagg atatcactca aaagtgatcg aacatggtgc cagctattct
     8461 agtggacaac gacagctgat cacttttgcg cgaacaatcg tgactgatcc taaaattcta
     8521 gtattggatg aagcaacagc aaatatcgat actgaaacag aaggattgat ccaagaagga
     8581 ttggcaaaaa tgcgtcaagg acggacaacg attgcgattg cccatcggct gtcaacgatt
     8641 cgagatgcag aattgatttt agttttagac aaaggtcata tagtagaacg cgggaatcat
     8701 gaaacacttc tagcgcaaaa tggtttgtat gcagatatgt acaaactgca aagcagtggt
     8761 atataaaaga tgttgtgaca gtagaatttt gacctaagca ctagagtgaa aataaaacat
     8821 agcttctcat atttttcatc tgtgagagtt agtgtcgtag tcaccagatt taaaaaaaga
     8881 ttcgacagct agtcagaagt gcctaatagc cactaagaaa gcgttgacaa aagcatttgc
     8941 tttcgataat ataaagaaaa cgatacaact tggatagtga ttgcctgatg caagctaaac
     9001 ctgatgagtc tcccatggtt tcatggacgg ctttttgggt ttttcttttt gttttggata
     9061 cagccctata catcatggaa aggagcaaaa catgctgatt gagaaagtac tgaataacaa
     9121 cgtcgtaata accagggatg aaagaaatca ggaaatgatc gtgatgggaa aaggtttggc
     9181 attcaaaaag aaagtgggtg acccgatttt tgactcatgc atcgacaaaa aatttcagtt
     9241 gacaaatgac gggctttcac aacagttcca agaattggca caagcgattc ctcttgagta
     9301 cttagaaatt tcttgtgaaa tcattgaata tacaaaagag gctctacaag tccagttgaa
     9361 tgattctgtg tatattgcat tgacggatca tttgtatacg gctatcgaac gtcacaaaat
     9421 gggaatattt gtaccaaatg tattgctgta tgatatcaaa caactatttc ctaaagagta
     9481 tgccatcggg aaaaaaacag tgaagaaaat ctcggataaa taccacatca agttatcaga
     9541 agatgaagcc ggatttatcg cattgcattt agtcaatgca caaggcattc aaagccagca
     9601 agacaatacc tacctgttta ctataacaat acaggatatt ctaaatatcg tccgctatta
     9661 ctataagatt acatttgatg aagattcagt ccattttttt cgattcgtca cacacttacg
     9721 tttttttctg acacgcgtcc tagatggaga aaaatcagaa gtgggagaag tttctgagga
     9781 attgtttcag gttattcaaa aaaaatataa ggatacgttt ggttgcgttg agaaaatagc
     9841 agacttttta gccgagacgt tcaactacca gatgtccacg gacgaaaaaa tgtatttaac
     9901 gatccatact gcgcgtttag tgcatgagaa tcgttgtaaa taaaaaaagg aggataaatt
     9961 aacaagaacg tatgaaataa gggtagtgac attcagttgg ctaaaccttc cactaataaa
    10021 ttggagggaa tagaaatggg aaaataccaa gaattagcga gaaaaatcgt agaaaatgtc
    10081 ggcgggaagg aaaatatcaa tggtttgaca cattgtatca caaggcttcg tttcaaatta
    10141 aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata tggacggtat tgtaacggta
    10201 atgcgagcgg gagggcagta tcaagttgtc attgggaatc atgtgccagt cgtgtttgaa
    10261 gaggttataa aagcaggaaa ccttactttt gatgaggctg tttcaacaga aaaaatgaga
    10321 ccgtttgata tgcttattga tggaatatcg ggctgtttcc agccattcct tgctatccta
    10381 gctgctggcg ggatgattcg tggactgaca gcattccttg tcttcttagg cgcgtttgac
    10441 cgcggttctg gcacatttgt tatgtttgat aatatcggtg attctgtatt ccaatttatg
    10501 cctgttatta tcggtttaac agctgcccgt aaatttaaag taaatgaatt cgtagggatg
    10561 ttgcttggcg cagcattaat gaatcctagc ttgtcgctgg aagctttgag tggtgcagca
    10621 gaagcaccac taacgaccat attttctggc actatttttg aagcacctat ttatcaaaca
    10681 gtattcggca ttccctggat tgcgcgtaac tacgcttctt ctgtcattcc cattatcttt
    10741 attgtgttgt tagctagcca aattcagaaa ccaattaaaa agttagtacc tgagatgatc
    10801 gcgaatttct tcgttccatt cttcaccgtt cttattacaa tgcctttagg ttttttactc
    10861 gttggaccag tattcacatt cgcaactgat attttaatgg ctggatttga aacactttta
    10921 gcactttcgc cagttattta tggagcaatc gtaggcttct tctggcaaat tctggtgatg
    10981 tttggtctcc attgggcaat cgtaccaatg ggattgatgc aattttctgt gaatggttgg
    11041 caaaatatta tgacacctgt tgcagtagtg tcttttggac aaactgcagc acttaccgca
    11101 ctctacttta agcttagaaa tccaaaagac aaagcaattg caataccagc cattgtttct
    11161 ggtattgtgg ggattacaga gccagctatt tatgggttta cattaccacg taaaaaaata
    11221 tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt atagtgggtt aatgaattta
    11281 acttcttgga atcaaggtgg actaggcatc tttacaattc ccaactatat tagaccagat
    11341 ggtgatttaa cagatgtgat taatgtattg attgggatcg ccattgcgat ggtggtatcc
    11401 tttacgctga cttttttctt ttggaaggat gaagcaggtg aaacagatga tatacagaaa
    11461 aaatcaggta aagaaattgt aaaaacaccg atacaaggac aaatcgctcc actaaatgca
    11521 gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa
    11581 aaaggagaag tacgtgcccc ttttgacgga acagtcatga ccctgttccc gacgaaacag
    11641 gcaattggaa tggtttctga aacagggctg gaattgctga ttcatgtcgg attggatact
    11701 gtgcagttgg aagggaaata ttttgaaagc ttagtgcagc aaggataaag agtgaaacaa
    11761 ggcgatctgt tgctacgctt cgacaaaaag aaaatcgaaa aggaaggata cagtttagaa
    11821 acacctgtga tcgtcaccaa ttacacggat tatttggata ttttggaaaa ccaaggggag
    11881 acggtcgggc cagacgatgc acttatcacc gcactgacct gaacggttag gtgaagaatg
    11941 gactaggctt aactattctt tttattccac ttctttattt tcaaaattga aaggctcttt
    12001 catgatggct gagctgatag ggcgtttatc gaatttttga ccgacagaaa ttagatggac
    12061 taaattctgt tgataatttt ctgcaaaaac acaactatat aacacgtcta gaagataaca
    12121 aattgatgta ttgatcgtaa aattaccaat ttttgaatac agtcgctcgc gtgtcgtaag
    12181 tcgcaaagcg gctgtactca ttgcagatag cgttgaatca ccgatactgg tcaaagctaa
    12241 aattggaaca tgattttgtt tcaatatttc tgcggtacga aggatcacat tgttttcacc
    12301 cgtataagaa atcagaatcc cgcacatatc actcgtgcaa ttgtacgctt catagaatga
    12361 ttcaccaaaa gtggtgacaa tcgtgacatc cattttgatg cgcttcattt tcagcatgaa
    12421 atcttgagaa atcaaagtat tcgagttact ggcgaaaatc acgatttttt ttgcttgaag
    12481 cattagttgt ttggcttttt gcagttggtc atgatgaatg agagaaagtg tatcatgaat
    12541 cgtcatctgt tctaaagaag ccattttatt tgcgatcgtc ataataccgt ctgtggtgga
    12601 aaaaggaaga ttcgcatcta cgtcttgaaa attcccttca agataggctg tttcagctaa
    12661 aaaagcatta cgaaaactga cccagccatc aaaacccatt ttttttgcta cacggatgag
    12721 tgtagagggg tggacataac atcgctcagc aatcgcttgg atggtcattt cgccaagtgc
    12781 tggagaatcg cttaagagaa actggatcac ttcattttct gaaggagaaa aggagatttc
    12841 tttgattttt tcacgtaata acatgtagat accttcttct tattttttta cgtaaattat
    12901 aactt
//
LOCUS       DAGCWU010000070        12790 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.070, whole genome shotgun sequence.
ACCESSION   DAGCWU010000070 DAGCWU010000000
VERSION     DAGCWU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.070"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..314
                     /locus_tag="MBZ71_001559"
     CDS             <1..314
                     /locus_tag="MBZ71_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429519.1"
                     /translation="FYNWNSWEKYTKNMKEIQKEFPQIVKSSPVDISGTMVGTGNSPI
                     SSYTADYYTTNQKGKMVELITQTRNTPTTATTTIWRGVTSIGENQLFYLEDLDSYVRA
                     E"
     gene            736..1473
                     /locus_tag="MBZ71_001560"
     CDS             736..1473
                     /locus_tag="MBZ71_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429520.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(1490..1906)
                     /locus_tag="MBZ71_001561"
     CDS             complement(1490..1906)
                     /locus_tag="MBZ71_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429521.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            2298..3545
                     /locus_tag="MBZ71_001562"
     CDS             2298..3545
                     /locus_tag="MBZ71_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBT4429522.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            3813..6203
                     /locus_tag="MBZ71_001563"
     CDS             3813..6203
                     /locus_tag="MBZ71_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBT4429523.1"
                     /translation="MKYEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            6252..8087
                     /locus_tag="MBZ71_001564"
     CDS             6252..8087
                     /locus_tag="MBZ71_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBT4429524.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            8156..9019
                     /locus_tag="MBZ71_001565"
     CDS             8156..9019
                     /locus_tag="MBZ71_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4429525.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRVMKNEE"
     gene            9009..10118
                     /locus_tag="MBZ71_001566"
     CDS             9009..10118
                     /locus_tag="MBZ71_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4429526.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            10457..11257
                     /locus_tag="MBZ71_001567"
     CDS             10457..11257
                     /locus_tag="MBZ71_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429527.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            11345..12151
                     /locus_tag="MBZ71_001568"
     CDS             11345..12151
                     /locus_tag="MBZ71_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429528.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(12173..>12790)
                     /locus_tag="MBZ71_001569"
     CDS             complement(12173..>12790)
                     /locus_tag="MBZ71_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4429529.1"
                     /translation="VADAGYKTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYD
                     AYFDCYLCPNNQPLLFSTITRDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIW
                     ADLMDELEHQRHTALNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLT
                     FAAMNLKKLATWLWKTKKPMAFYFKIMQRLTKRSGLITNSFLSTI"
BASE COUNT         4775 a         1883 c         2521 g         3611 t
ORIGIN      
        1 tattttacaa ctggaattca tgggaaaaat atacgaagaa tatgaaggaa atacaaaaag
       61 aatttcctca gatagtaaag tctagtcctg ttgatatttc tggcacgatg gtaggcactg
      121 gtaatagtcc aatatctagc tatactgctg attactatac aacaaatcaa aaaggtaaaa
      181 tggttgaatt gattacacag acaagaaata ctccaacaac agcaacaaca acaatttggc
      241 gtggagtaac aagcatagga gaaaatcaat tgttttattt ggaggattta gatagctatg
      301 taagagcaga gtaaaataaa aaaagagcta acggtaccaa cgttagctct ttgtgctgac
      361 gatgataaca aaacctaccg aaaaaggatt gtttcatcac aaacacaaac agaaaagaat
      421 tgataaaaaa cccgaacctc agaattttta ttaatcaatt catttagatt ataataaatt
      481 gagctgaaca ttacaatacg tatatgttgt aaaacgtaaa aaaagtcaaa agtaaatact
      541 ttcttaaaaa gtcaaataaa actattaaag acaatatata tggtttattt gacataagaa
      601 aatggtctag atgataattg taatatagaa ttgtagactt aaataaagca taaaatacct
      661 tttatgataa ggtatagctc ttaattttaa gcagttttag atataagatt gaaagtaaag
      721 tgggggagaa taataatggc agatcataaa ttagaaaagt tattagaaga aacgtggaat
      781 ccaaaagaat ttagtgaatt ttttatggag aattttgaaa ctgacttagc agtgattgtt
      841 aaagatgctc ttagagagca agggtatcct gaaactgcaa actatataaa cattaatttt
      901 actttgtata ctgaaaataa aggaacttgg gatttttggg ctactttagc aaataaggag
      961 ttaagtgata aatcagacac aggaattcgt aacttttttg aatcaaacag agatgattat
     1021 atgtatgcta acaatcaaaa taaattgaac tttagagtag agtttgatga aacacctgaa
     1081 gaatttatag aaagacaacc gcctaaagaa aatgtagcaa aggtattgga agatcgttgg
     1141 aattcagatg aaatagtttc aacaattagc gaactaggcg gacaatatga accgctagta
     1201 gaagcggtta gagaagagct aagactgaat aagtttcctg atgttcaaaa tattgatgtt
     1261 tctcaaattg aaataaacgt caagatcacg aataaacttg attatggtag ttgggcagat
     1321 atagcattag aaaaatacat ctactcaacg ttaaaagagt ttatcgaaaa tagaatggat
     1381 atcatgtatc tacagcatcc acagtattta aactttggtg ttgaaatagc tacacctctt
     1441 gaagaatgga aaatggagca agggcttgac tagcctttgc tccatttttt tattttacaa
     1501 atgaatacag ttcgtcgttt ttaccaagaa tgattgtttg cccaactttg agattgtcag
     1561 gtgatccttc agcggtttgt aaggagaacg ttccattgaa cttatcggat accgttatag
     1621 gtagctttaa ttctgatcct tcctcatctt tgagaattgc tgtttttcgt tcatcaaaat
     1681 aaacagtctt tattaaataa tttttcgtag tgatctcagc agatttgtct ttttttgtag
     1741 tagtaccagt ttctttagaa ttagaggtat tttctacgac tattccatct ttggcatttg
     1801 agcttgtgtt tatttgattg aatattttca gcgacaagat aacgctaaga atagtcaata
     1861 tagaaacaag tgtaattaat actgataatt tccttttttt gttcattttt tcttaacatc
     1921 cttttttgtt ttgattagtt agatattcct tatctaacaa gcctttttag gctattaaat
     1981 gaagataaac cccaaaaaat attgcaatta tgacagtgca atggtactat tattatacaa
     2041 taaattttgc gaaaggggta ggtctaaatc gcaaacaaaa agaaattgaa cctagacaaa
     2101 tacaacttgt ataccgcaag ggaagcaagt ttgattttgg gtaaaggtag aaattatgtt
     2161 acaaacataa tcaactatta tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt
     2221 gacaaaacct atattataac ggaagaagga atagaagcat tgcgatcaca aacagaaaat
     2281 aaaaacaaaa agaaggaatg aatatgaaga gaaattcgct tggtatggtt ttattggcat
     2341 cagctgtact tgcggggtta tcagtaccag taacctcaca tgctgatgaa tggactgcaa
     2401 acacaccgga tcaaatccaa attacaacag gacaatcaag ttatgcgttc aaaaaaggag
     2461 atactatttg gtcaactgta gagaagctta aaagcttagg ttacaagact actgttgctg
     2521 agtttgccga attgaacaat gttgatttgg caaaaggaga acaacggact atcccgattg
     2581 gacgaacttt caacatcagt aaagatacat taacagttaa ggaagcagat ggcgaagtag
     2641 ttagtcaaac tgttcaaaaa gataaacctg atcctattga aaatacggag caagagaaca
     2701 aaaaatccga gactacaaaa gaatcggaac caacagcaaa cacttctgat aagacagaaa
     2761 caagtgcttc aagtgttgat ggagcaagtt catctaatac aaataattct acaagtgaga
     2821 aagaagcatc acagggaaca agtgaaagta gcactacgga agctgatact ggaacatcaa
     2881 caaataactc acaatcaaca tcaaatgatg caaatgaatc aaccagtaca tcttctagtg
     2941 atcaatcaca aacaggttcg agtgatgaaa ctgacagttc tacttcacca agtaaacccg
     3001 aacaaccaac taatgttgtg ctaggcgttc catttattag tcaaggtaac acaatgcttt
     3061 gcgaaggaac atctttgcta gaagcgttac attataaagg tgttgcaaca aatcaagatt
     3121 taatgacttt tgttaaatca atgccactat ctcctgacaa taatccaaat aatggttttt
     3181 ctggtgaatg gagacacaat gtggatggaa cttatcaagg tatgaatccg gctcctgtag
     3241 tttcatgggg tgttgctaat ggcggaaatg ttgtagatat ttctggttca tcggtacaag
     3301 gattaaaaga tgagattgta aatggaaatc ctgtagtagc gtgggtaact tatgcctttg
     3361 aacctgctca acataaacca atgttttggg gcgatgctat ttggaatcga catgtagtag
     3421 ccgttgatgg atttaaagac ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt
     3481 ggattaatgc aggatcattt gaaacagcat ataacgtatc tcaattagcg gtttcggtac
     3541 gttaataaaa gatatcgttt atatactaag tagttatata gtctttattt gtccttgctt
     3601 aactacttag aaaacttgtt gctcatggtg ggtagaacta aaaggggtga tgctatgaat
     3661 actagagcgt tgaaacagct tgctttcaac gaaaaaaatg attaataggt gtggtgattt
     3721 taacttagga atgggcgcga aggtcattac cacaccgaca tatcgtggct cattttcttc
     3781 gcctaactgg tataaagaag gagtaacgag agatgaagta tgaaaaaacg ataattttaa
     3841 aatcaaaaat aatttattgt tttgcgactt tagtaatgat gttatctacg cttataatgc
     3901 ctttttcagc aataagagct gaagcagatg tcacaaaacc gacaagtgca aaatttatag
     3961 aaactggcgt aaaaacagac ggttatatta gggttaatat gccgaatcat ccaaatgaat
     4021 ggatgatttc aagtcaattt aaagattctc atggaaatat tggatattgt atggattcag
     4081 aattgccatc cccaacagga agtggagccg gaagcttgaa gtacaaggga gctggcagtg
     4141 atgagttcta tcgtatgttt aaaggtggtt ttccatcaaa aacagcaaag gaactgggtg
     4201 caggaaatga tacagaagca tggtatgcaa cacaattagt ttcatgggtt ttagccggaa
     4261 actttaaagt ttcacagatt gtatggtcac atccaaacca taccgccgca gaaacagctc
     4321 gtgtaaaaaa agcatttgaa aaaatttatg actatgctaa gaatggaaaa gatacgccca
     4381 acactgagtt ttctatcaca gcttctaaga cagctgacga aggaaaatat cacactttca
     4441 catataaaac agcatcaaat aagactggta atgcgaagct cacttttaca tcagcaaaac
     4501 cagctggtat gaagatttat gatgcagatg gtaaagaaat tactaacaac actgtgaaat
     4561 taaattcaag ttttactatt aaagtacctg tgactacacc aagtggtact ttgtcattta
     4621 aaggtactgc taatgttagc acaaccaatc cttttacttt tgatggacga ggtgtgtatc
     4681 aagatgccgt agttatgatc actacttcag aaacaaagga ctctaaatct ttgagtgcta
     4741 aatggacaag agctgttggt caggtaacag ttaagaaagt tgatcaagat ggaaatgctt
     4801 taaaaggagc ggagtttact ctaactgaca ataacaatgc taaatctgtt aagacaaccg
     4861 gagatgatgg gttagctacg tttgatttgc aacaaggctt aacatacagc ttagaagaaa
     4921 caaaagttcc tgaaggttat caaggttcat ttaaaaagga tggtattact gttaataccg
     4981 atggacaaga atttagttac actgctacga acacaattaa aaaaggtaaa gtaaaagtta
     5041 aaaaggttga ccaagatgga aaaccattag ctaatgctga atttaccttg acggatgatt
     5101 catcccaaac acaagcagaa aaaacagatg aaaacggact tgcaagcttc gatattgtag
     5161 caaacaagac ctacagcctt gaagagaccg gagtaccaac tggttataca ggcgtattca
     5221 agcaagaagg aattaccgtt aaagatgatg gacaaacttt tgaatataca gctacaaaca
     5281 ctttgaaaaa aggtttagta aaagttcata aagtcgatca agatggaaaa cctttggcaa
     5341 atgcagagtt cactttaacc gatgaaaaag gtgcaacaca agtagaaaaa acagatgaaa
     5401 atggacttgc aagcttcaat attgtagcaa acaagacgta tagcctagaa gaaaccggag
     5461 taccagtagg ttacacaggt gcatttaagc aagagggtat caccgttaaa gacgatgggc
     5521 aaacttttga atatacagct acaaataatt tgaaaaaagg tgtagtaaaa gttcataaag
     5581 tcgatcaaga tggaaaacct ttagcagatg cagaattcac cttaacggat gaaaaaggtg
     5641 taaaagaagt taaaaagacg gatgcaactg gtctggctac gttcaacatt attgcgaaca
     5701 aaacttataa cttagaagaa acaaaagtac caacaggtta cacaggcgca ttcaaacaag
     5761 aaggaattac cgtcaaagat gatggacaag tctttgaata tactgctaaa aacactttga
     5821 aaaaaggtgt gatcaaagtt cataaagtgg atgatcaagg gaaagcatta gcaaacgcag
     5881 aattcacttt gacagacgac aaaggtgtaa aagttgtaca aaaaacggac agcaatggct
     5941 tggcaacatt taatattgtt gccaacaaaa cgtacaaaat cgaagaaaca caaaacccta
     6001 atggttatac agggaaatgg tctaaagaag gcatcaccgt tgctgacgat ggacaaacct
     6061 ttgaatatac agtaattaat caccttgtac ctgaaacagt aagaacaggc ggaatcggta
     6121 catcagcctt ccttcttgta tttctaattg caggattgag tatggtgcta ctgtattatc
     6181 gagaacagca caaagcaaaa taaaaaagtg ttaatggaaa agaagtaaat atgaaaaaag
     6241 aaggctgacg tatgaaatca aaaaaaattt tttcaaattt aatgctattt ggtactttgc
     6301 taggagtggg attgggcgca agctcaatct cttctagcct tggactaggg gggcaagtag
     6361 catatgcaca acaaaactat aattatcaag gaactggaac gattcatatc cataaagtaa
     6421 caaatactaa tggggatggg aaccctgtct cagatattga taacaaaggg aaagaaatag
     6481 ctttaccaaa tggtgtaaaa cctcttgaag gcgttgagtt ctcttatgtg aaacttacta
     6541 aagatgaagc taaatccttg ggatggacgg atagtgatac tacaaaaggt caacatattg
     6601 atgatgctaa agcacgatta ttactaaaca aaaaatacag tgaagtaagt agaatgaaga
     6661 ctgattcaca agggaatata gacataccta tacaagtaaa tacagacgat ttaacagata
     6721 acttgtattt gattgttgaa acaggaacag tcaaaggtgt agttgaagaa gcgtatccta
     6781 tgattgttaa cgtacctgaa ctaaacggat cagatgatca atactttaat tatgatgtac
     6841 atgtctatcc taaaaactat ttccaattaa actcagcaga gcttgataaa aaagacgaag
     6901 atggaaaggc tcttgccgga actgtttttg aattgcgaaa agatgatgga tctgatcagg
     6961 ttgttggaac ttataaaaca gacagcacag gtaaattaac tgtagatggt ttggaattag
     7021 gtgactatta tttcctagaa accgatttgg gaacagatgc taataaaaca acctatttac
     7081 tagatggtaa aaaattcaaa tttagcgtta aacgtgggga gactgctaaa gtagttcttg
     7141 atgttaccaa ctacaaacga cctgtaatta ctaaggtaat tgataaagaa accgttgata
     7201 ctggcgaaga tgttcaatat acattgaaac catcaattcc tggtaatatt ggtaattacg
     7261 aaaaatatgt tgtcaaagat gttctaggtg cagagttaac ttatgattca tcagaagtga
     7321 tcgaaggaat gactttagat actgattaca gtatcgaacg tgacggacaa acgacaaact
     7381 atgtctttac tgaatcaggt atggataagc ttcagaaaca atttgatgca ggggatcgct
     7441 tctttacaat tttgatgaac gctcatacta atagtaaagc taaaatggat acaccactta
     7501 agaatgatgt ttcaacgatc tacaacaatg gatatgaacc cgaagacgaa acaccagttg
     7561 atcacgctta tacaatcatt ggtgatcacc aattcattaa aacagatgga gatacaagca
     7621 aagcattgag tggagcaaaa tttgtagtga agaacaaaat tgatccgaat aaagttactt
     7681 acctaaaaca agatccaaca acgaaatctg ttacttggat tgcagataaa tctaaagcta
     7741 ctgaattcgt ttccggatca gatggtaagt tccaaattga aggcttgcaa tatacctatg
     7801 atgtagattt tgataaacaa ggtaatccaa caatcacaga taaaaaaatc aatgattatg
     7861 ctcttgaaga agttgttgct ccttcaggat atacaccaac tagaaatgat gttccatttg
     7921 tggtatcgaa tgatgatgct tctagcgaag taacagacat caagaacctt aaaacacctg
     7981 aaacagttcg tacaggtgga attggaacag tagtatttct agtggttgca ggtgtggcag
     8041 gaacagggct atatgttgtc tacaaacgag aacaacacaa agcttaatga aataaaattc
     8101 cctagagtgt ttttggctct agggtttctt tttaaaaagt gtgtgaaaag taggtatgag
     8161 catgaaaaag aagaatatca agttgatcat cttaaaaatt gggatgttgc tatgtttagg
     8221 agtaggtcta tatcctgtaa ttactcaggc ttatacctac taccaaactc aacaaacact
     8281 aaactcctat aaggaaaaat atgatgaagc ttctgaaaag gagaatgaag aagtcttcaa
     8341 gagatattca gaagttaata ggaaacgaaa agacacagaa caaacctcag tcacgattcc
     8401 tgaagataat aacaatattc cgtcaaatgg aaatcttcag aaggataaat taggtgaatt
     8461 agtgggtgaa attcttatac caaagattaa tgtggatttg ccaatttatg atggtgtaaa
     8521 cgatttccaa ctatcagcag gagcaggaat attaaccgga actgattatc caactggtgg
     8581 gattggaaac cattctgtta tatcagctca ttcaggcatt cctaataaaa aatattttac
     8641 agatttagag ttgctcaaac ttggtgataa attttttatt gatatttcag ggaaaaaact
     8701 tgcttatcaa gtagatcgga taaaaacaat tctaccaacc gaatttgagg atttgcttcc
     8761 tgtggcagga aaagattatg taacacttat gacatgtaca cctgttggag ataattctca
     8821 taggttactc gttagagggg ttagcatacc ttaccaaaaa agtattgttg agaaagaaaa
     8881 gaagaaagaa gatttaaagg taaaggcgca gatgaactgg tatcctatcg tagttgtagg
     8941 ggttttatta ataatagctg tgatagtcat tattaaaaaa atagtagcaa agagacgagt
     9001 aatgaaaaat gaagaataaa atactacttt ttttgactct tatttgcact ttggttttaa
     9061 caatttatcc tgcaaatcag ctctatcatc aattcaaaac aaaagagaat gtggcggaaa
     9121 tccaagagaa aattgagaaa aaacaaactc cttcaaaaga ggaagtggca aaaaaagtcg
     9181 aacaaataaa atcgaacagc ggtaaccaag gattagacaa tactcagaaa acagataaag
     9241 gagtagacaa tactcaggaa acagacggtg gatcatttga tattcgtgaa gctgaaggaa
     9301 aggtcaatcc atacacagga gatgtttttt caaaaaatca aacgaaagat tttgaaccat
     9361 ctacctattc aggagcggtt ggttatgtct atattccaaa aataaatgtg ctagagcttt
     9421 tgtttctagg cgcttcacag gagcatttat atagaggtgt ggcacaaatg agtgctacta
     9481 gtctaccttt atcaggtaaa ggtcatcaaa cagtaatagc aggtcatcgt ggcggttata
     9541 cgtcaccaca atttctttat gttgatcaat tagtagccgg agaccacatt tatatagatt
     9601 atcttggtga aaaagcagtt tatacggttt tccgaacaga cgttattaga aattacggtc
     9661 agaattcaac actgactgat attcctgatt tagatatgtt aacactttat acttgtcatc
     9721 catatcctca gaactacgat cgtttattag ttagaagtct aagagatgaa aaagcaaaat
     9781 acatcacgcc acaagtaaca gttggcaata ccgcatacgc tgatgccatt gatagcgagt
     9841 ccgtttcaga taagacagaa actgttacat cttctaatga tttatttgaa gatgttgatc
     9901 gtgccaacaa ggaattagat ggaaataatg atcttaatat tccggaacaa ggtagcgtag
     9961 agatgcaaga cagaacgttt gctgaaaagg caagttcaat atttacagga attaccaata
    10021 aacatcagaa aatgctttta gtcatttgga ctgtaatagg agcattagca agtctaaagg
    10081 ttcttattct tttaatcaaa gagtttcgaa aagcataatg aagattataa aaattgatag
    10141 agtacagtaa aaagggattg atactattat tgatctcttt tttattttga atagagctag
    10201 attcactttt aaataaaatc aaaactgatc ttaattctaa agaaaaagtc aagaaaaaaa
    10261 tacaataaat ttttttatca gaatatttat cagaatatta ttagaaaaga taaactcaaa
    10321 agcttaattt aatagggtta ttggttgtat ttagaaataa tgtatggaaa agctataaag
    10381 caatagataa gatgtagaaa atcttatttg tgaatgaaaa acaagtcatc gtaaaatatt
    10441 aataggaggt gtagtaatgg tgggagtatc aaaaacagct ttagactcat tagctaaact
    10501 atttagtagc aggtttgatg gtgcagaatt tgttgaatat ctaagccaaa acgaaagtgg
    10561 aaaaactctg atcgaaacaa tgaaaaaaga ctatttgaat gaaaatgact ttccgacatg
    10621 gacgaatatt gaagatgcta catgggatgt agtatgtacg atatattctc cttgggtaac
    10681 gattgctgat tttgttttag ataaaattgg aggggataca taccctgatg ccaaatctct
    10741 tgctacaagt gactttaaaa cgttctatgg ttatctaata ggtctaatag gcaatggaat
    10801 agatttgaaa gttacgccaa aacaaacacc tgaagaaatt gaaaaagaaa aaggagaggt
    10861 tcaaatggct tatgcaaatg acagagaaaa agtaaaatta ttggaaggta aattatctga
    10921 agaagaactt ggaaaaatgg tgcaagaaat caatgaagac agcaactatt ttcctgaatt
    10981 gaaggtatat caaaatgacg aagatttctt taatgaaatg tttagtaata gtccatggga
    11041 agcagtaagt aaaacaaatt taggagaatg gaactttcaa gatgatctag ttgcatttag
    11101 caatgatggg tatttacgat catattcaga aagtgagtat agagctgaat taaaaacgta
    11161 tgaaacggat attctgatga gctatttaaa tctattagag agtggtcagg gagagttaag
    11221 agataaagtt gaagaactat taaaagaaga gctataggga ttatttcaac ataaacacaa
    11281 gcagaaaact accaaacaaa acgtaccatg caaaaagtcg gttaaaccgg ctttttgctc
    11341 tattatgaga ggagatcaaa ccatgagtgc aacaaccgat atggctttag aagcagtaat
    11401 aaaagataat acaggaaatg tcgaaaatgt agaagacttt agcgatattg caatcaatct
    11461 ttcagcatgg atttcagctg taggagaaga aaatatcgaa tttgagatca agaatatgga
    11521 tgacttaaaa gcaaaatttc cttttattgc atcgaaacta agtcaagttg ccgaggataa
    11581 atcgtggaat gaattagtta gtttttccga aaaaatttat gatattacgc aagaagatga
    11641 gtgtgtttca tgggaaatga ataatcaata caagaaaatt gacgctttcg tcaaatcacg
    11701 tatagaagga acagcttttg agtttgaagg tatagatacc gcagataatt ggttaaataa
    11761 agaatccttt attaaaagta tagcggatga tcttgaaaaa caatggtcta aggaagacta
    11821 tgcagagtac ctaaaaacag aaagagatac agaagtgaaa gaaaaactca ctgaggtatg
    11881 tttggatgaa catattgatc ccaatatcgt aaaacaagaa gactatgaag tggttattga
    11941 taaagttcct actgattttg aggaagttgt aaagtatata gttgaagaag cattatcgca
    12001 atactcttca gatgaggaag tggacgaaga aactgttcgt accagagttt ggcaactggt
    12061 ttttgatgac aaagattata aatatttgag tgatcaaaac tcggttaatt ggagtgtacg
    12121 attattaatt gatcaaaatg atattgaata aaatagagct gaaaagctct attcagattg
    12181 tagacaaaaa ggagttggta attaagccac tcctttttgt tagtctttgc ataattttga
    12241 aataaaaagc cataggcttt tttgttttcc atagccatgt ggctagtttt tttaaattca
    12301 tggcagcaaa cgtaagcatc gtgtgcgtgg cgactttttc aagacctcga tatttcgtcc
    12361 aacgcatacc atgcttttct tttgcatctg caaatattcg ttcaatggtt tgttttctct
    12421 ttttgtaaat ttctctattc aacgccgtat ggcgttggtg ttcaagttca tccataagat
    12481 cagcccaaat atgtcgagtc actatttttt gatgttttgt actcaaagta caattttcaa
    12541 gtaatggaca actcacacag aattttggat ttgatttata ggttcgataa ccatcacgag
    12601 tgatagttga aaataacaac ggttgattat tgggacataa atagcagtca aaataggcat
    12661 cataaacaaa gtctttttta cgaaacattc ctttcactcc atgaggtgca gtatatggaa
    12721 gaatcgggta gagtttcaag tgtgacagaa aatgaacaaa tttaggcgtc ttatatcctg
    12781 catcagcaac
//
LOCUS       DAGCWU010000071        12689 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.071, whole genome shotgun sequence.
ACCESSION   DAGCWU010000071 DAGCWU010000000
VERSION     DAGCWU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.071"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(718..3867)
                     /locus_tag="MBZ71_001570"
     CDS             complement(718..3867)
                     /locus_tag="MBZ71_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBT4429530.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="MBZ71_001571"
     CDS             complement(3880..5079)
                     /locus_tag="MBZ71_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBT4429531.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..6664)
                     /locus_tag="MBZ71_001572"
     CDS             complement(5069..6664)
                     /locus_tag="MBZ71_001572"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBT4429532.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(7248..7955)
                     /locus_tag="MBZ71_001573"
     CDS             complement(7248..7955)
                     /locus_tag="MBZ71_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429533.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(8020..8856)
                     /locus_tag="MBZ71_001574"
     CDS             complement(8020..8856)
                     /locus_tag="MBZ71_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4429534.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8874..10283)
                     /locus_tag="MBZ71_001575"
     CDS             complement(8874..10283)
                     /locus_tag="MBZ71_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4429535.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(10297..12120)
                     /locus_tag="MBZ71_001576"
     CDS             complement(10297..12120)
                     /locus_tag="MBZ71_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4429536.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         3722 a         2672 c         2061 g         4234 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt
     6481 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     6541 atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg
     6601 catccatttt actacggaga ttatctgccg cactaaataa tttttgattt aattctgcac
     6661 ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt attttagcat
     6721 accccctttt ataaaaagca aaaaaaaaag aaagaaactt attcttgcta ataaactacg
     6781 cgaaaggagt ctaacgttgt ttgatataac ttagactgat cgttaactta ttttttatat
     6841 ttctacatag ttatctttag aaccatttca gatacatatg aaacaattac atatgatttt
     6901 ttcataaaaa aacagtactg tctagtagta taaaatatac aaatttctta aaaatttttt
     6961 cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt aatgaaacta
     7021 tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga aaaaaaatac
     7081 ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata acaacggggt
     7141 agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt aaatacttcg
     7201 ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatcttttta cccattttca
     7261 agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact gatacctgtt
     7321 agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt atcagtattt
     7381 atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc tgtgtatgtt
     7441 tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc tgtctcatta
     7501 ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc tgtctcatta
     7561 ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt agtgtgcata
     7621 gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc aatagagact
     7681 tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag cattttgttg
     7741 tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg gaatacagct
     7801 ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc tccaagtagc
     7861 tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt agaggtcgaa
     7921 acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt agcacaacca
     7981 acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc ttgaaatatg
     8041 cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt cttgaacgta
     8101 ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca ctgaagccag
     8161 caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa taaaaaactg
     8221 taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt tttcgggaaa
     8281 cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca tggtttcttc
     8341 catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc cagcctcctc
     8401 ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc agccgatctc
     8461 aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa aaacaatgcc
     8521 tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa atagactttt
     8581 attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga cgcaaataat
     8641 tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag catcagaaag
     8701 tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc ctttctttag
     8761 ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat ctaagcaaag
     8821 aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc tctctaagct
     8881 aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt tattcgtttt
     8941 aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg cttattgaag
     9001 ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa aacatctaca
     9061 ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc aattcgatcc
     9121 tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc attttcagta
     9181 attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg tagtccagta
     9241 gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc tgcccggtag
     9301 acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac atctgatccg
     9361 tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat aaaatcgggt
     9421 ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa tccacgatca
     9481 ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc taagaaagac
     9541 cagcctctca atgtttccca attatgggct gcaattgcat cttctggact ggaggtagct
     9601 tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt caactgatga
     9661 tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg aaagagttct
     9721 ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg aaggatcatc
     9781 gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc agtaagcaat
     9841 acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc gccctgctcc
     9901 tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat tccctgattc
     9961 aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt tttattattt
    10021 tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa gcctaattct
    10081 ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac ttcaaaatta
    10141 gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc atcctcctga
    10201 taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt ctctggaaat
    10261 gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca tcaatggttc
    10321 gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac tgttggtcac
    10381 taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat cggcgttgat
    10441 taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa agggttcccc
    10501 ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt ctttagaacg
    10561 tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa caggtgaata
    10621 aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct tagaaaagac
    10681 agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt caacaatgac
    10741 tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc caattagata
    10801 agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt gaacaatatt
    10861 cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa caaaggcata
    10921 tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa tcatggaagc
    10981 aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag taattcccat
    11041 taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt tagttttcgc
    11101 ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc ctggcccatt
    11161 gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca ttgcccaagc
    11221 tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa ttaaccaagc
    11281 tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca acgttccaaa
    11341 cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca tcggcttagt
    11401 aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat aggacatgat
    11461 ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta ataccggaat
    11521 tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg gatgaagcaa
    11581 aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg ctgccccata
    11641 ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca aaatgatgta
    11701 agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa tggcttttac
    11761 catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa tgacactaat
    11821 caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa ttgaagttcc
    11881 cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca ccacttggta
    11941 ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg ctttcgtatc
    12001 ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg ttaagctgac
    12061 cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc tgtaatccat
    12121 tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca atcaacattg
    12181 aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata acgttatttt
    12241 gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt aattttttat
    12301 cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta gggtatatct
    12361 gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta aaaccattcc
    12421 tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat ataacggatc
    12481 acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag taaaggatgg
    12541 aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt tggtaaaaat
    12601 cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcaata gaatgatttg
    12661 ggctagtatt cttaaaaatt aactagcaa
//
LOCUS       DAGCWU010000072        12582 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.072, whole genome shotgun sequence.
ACCESSION   DAGCWU010000072 DAGCWU010000000
VERSION     DAGCWU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.072"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(178..585)
                     /locus_tag="MBZ71_001577"
     CDS             complement(178..585)
                     /locus_tag="MBZ71_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBT4429537.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(580..1784)
                     /locus_tag="MBZ71_001578"
                     /pseudo
     CDS             complement(580..1784)
                     /locus_tag="MBZ71_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            1910..2128
                     /locus_tag="MBZ71_001579"
     CDS             1910..2128
                     /locus_tag="MBZ71_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4429538.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2149..2784
                     /locus_tag="MBZ71_001580"
     CDS             2149..2784
                     /locus_tag="MBZ71_001580"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4429539.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2977..3216)
                     /locus_tag="MBZ71_001581"
     CDS             complement(2977..3216)
                     /locus_tag="MBZ71_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4429540.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3240..4763)
                     /locus_tag="MBZ71_001582"
     CDS             complement(3240..4763)
                     /locus_tag="MBZ71_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBT4429541.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4781..4903)
                     /locus_tag="MBZ71_001583"
     CDS             complement(4781..4903)
                     /locus_tag="MBZ71_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBT4429542.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4933..5916)
                     /locus_tag="MBZ71_001584"
     CDS             complement(4933..5916)
                     /locus_tag="MBZ71_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4429543.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5940..6089)
                     /gene="rpmG"
                     /locus_tag="MBZ71_001585"
     CDS             complement(5940..6089)
                     /gene="rpmG"
                     /locus_tag="MBZ71_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBT4429544.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6110..6487)
                     /locus_tag="MBZ71_001586"
     CDS             complement(6110..6487)
                     /locus_tag="MBZ71_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429545.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6519..6698)
                     /gene="rpmF"
                     /locus_tag="MBZ71_001587"
     CDS             complement(6519..6698)
                     /gene="rpmF"
                     /locus_tag="MBZ71_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBT4429546.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6713..6982)
                     /gene="rpsN"
                     /locus_tag="MBZ71_001588"
     CDS             complement(6713..6982)
                     /gene="rpsN"
                     /locus_tag="MBZ71_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBT4429547.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7505..9271)
                     /locus_tag="MBZ71_001589"
     CDS             complement(7505..9271)
                     /locus_tag="MBZ71_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429548.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9231..10985)
                     /locus_tag="MBZ71_001590"
     CDS             complement(9231..10985)
                     /locus_tag="MBZ71_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429549.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11095..11664)
                     /locus_tag="MBZ71_001591"
     CDS             complement(11095..11664)
                     /locus_tag="MBZ71_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBT4429550.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(11682..>12582)
                     /locus_tag="MBZ71_001592"
     CDS             complement(11682..>12582)
                     /locus_tag="MBZ71_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4429551.1"
                     /translation="DFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKN
                     RTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINF
                     DVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFF
                     TESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKT
                     NLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGI
                     KKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         3963 a         2414 c         1790 g         4415 t
ORIGIN      
        1 aataagtaat ctaaccgact gttaactaaa ccattgcagg tcccgttaaa gatacttgtg
       61 cctgttgtca ccgcttcttt ttcttgtcca caagcgctta acacacttaa taaagcccct
      121 gctattatta ataataatat ctttttctta ttaaaaacct cctcatctat ttccagatta
      181 gtaaatgtag taatctttgc tttctttttt ctcaatacta ataactaact tatggggtaa
      241 acaaatactc gtttgaccgg gttttgagat ccatccagtt ctaaccgcga tttgatcagg
      301 actattatct tctttaactc gaattcttcc atctttaatc tcaacaatat tatattgtcc
      361 cttcgcagga tgataattca cgagtttatg attgatctta tccaaatcga agcgatccat
      421 ttccttgcca tcaatttgaa cgatggcata tttgatagca tcttcagttg tttcattctc
      481 cttttctata gagaatacaa ataagggaat aaaagaggga ataatcaaaa tgccaataat
      541 tatgacatcg cctatcttga caccagcttt tcttaaaaat ttcattaacg atatctggct
      601 aaacatatta gaatttttcc atggaaatgg tgaaattttt cggaaattat ctgcattaag
      661 agaatcagtt aaacaaccct caagttgttc cttaatacat tgttgatcca tcttcatacc
      721 aataaaaact agtttattaa tgcgttcacc aaacaacgga tgccaggctt ccttcaattc
      781 gggttttttt gtataatcat ttgacactca attttaggta aactagctac ccaataattt
      841 aatgattcaa tacttttatg gctacccgat gtttcaaaga tcgtcgaaaa atcactatgt
      901 tgggcaaacc aaagaaaccc ttttgctctt gtaatattgc caggaaaatt tttatccgta
      961 agatgggcaa atttttgtgc gtcaaggagt aaccgatttc gatagataaa acttgagata
     1021 ccatattctt ctgtttcagg aatatgattt tgatagccaa gctctaactc tttgaaccag
     1081 cccattgaat aggaagtctc atctatatta aaaagatttg tatttagaat tttcgataag
     1141 tctacttcac ctttaattgt cctaattatt ttggcattgg actgtaactt agcaattaga
     1201 ccgttaattt tgtaaatcgt cttggttgac actaagtcac tattatttaa aagtaatatg
     1261 ttacaacact cgaattgatc aattaacaag ccacgaatat cctgttcttc ttcaactaca
     1321 ttaccttcag cttttctttc ctgaatcttt tcacccgttt cgaaattatc ccaaaaacgt
     1381 tttacatcca ctactataat aatggcacca atctctaatc gttgggtcaa atcaatgtct
     1441 aaagggtttt tagctaatat aattgcttga ataattggaa ttggtttgct gattcctgaa
     1501 gcttcaataa caatttgatt caagtcagaa aattgcacta attgatctag ttcaacaatc
     1561 aaatcttcgc gtaatgtaca acaaatgcat ccattcggca tcgaaacaag tttttctcca
     1621 gttcttttaa aacctttctt gtcgactaat accccatcaa catttacttc ccctatgtca
     1681 tttacaatga cagcaatttc ctttccgtga tcaccgtaca gaatatgatt tagaagtatc
     1741 gtttttcctg aaccaagata gccagttaag attgtaatag gtattttttc atagggaacc
     1801 tctttctata ccttgaattt acactttaag tgcaacacat aaaaatgagt cataatcgat
     1861 atccaactag tataagtttg tcgagaacac tcctagaagg aaaataatta tgagccagta
     1921 ttgtcatctt actatactca aacgagaaag aatctttctg cttcacgaag aaggattttc
     1981 tattagtata attggcaacg acattcagca tagcccgttt actattttac atgaactgag
     2041 aagaaacaag aaaaaaacta atcaccttcc aaagcggaaa aaaattatgc ttatcgaaga
     2101 aaaaaatgcg gacgttatca cattctaaat gatccgtgag tttggtcaat tgttcgtcat
     2161 ttatttgtcg attttcagtg gtcgccagag aaaatctcga atcgtttgaa gcatgaacat
     2221 atggactttt caattagttt tatcacgatc taccgtgcaa tttgcaatga tcttttcgat
     2281 acagaacccc tctcacacgg caataaaggc ctgatccgtt cattgcacca tcgaggaaaa
     2341 acaagacata caaaaaatca tgtcgagtct cgtgggaaga tttgccttac tcattcgatc
     2401 catggccatc caaaatccgc agatgataga agtgattttg gtcattggaa agccgatatt
     2461 gtagctggca aaactagtgg tacttgttta gtgactttga cagacagaaa atcatgcttc
     2521 cttttagcag gaaagaaggt ccccagaaag aaagccgctt atgtgagcga gaaaatgatt
     2581 gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa
     2641 gaattctcca atcatttcgc agttacagaa gtattgaata atatgccctt ttattttcca
     2701 gatcctcatg cccttggcaa caatgaacaa attaaaatac gaatagactc attcgagaat
     2761 acttgccaaa atcgaaagag atagctacgg tagataactc aacgataaat cagatgattg
     2821 aaaaactgaa taacagacct agaaagtgtt tagattggag aacaccttat gaagtcttct
     2881 ttgataaagt gttgcgcttg atttgacaat tcaagaaata ataaaaagca gatcaaatcc
     2941 aaataaggga aaaatgatct actctggtat tatcaattaa ttggccaagc tattatgccg
     3001 ttttgctaaa cgtctacttc ttccagtatt gaccttgata aaacaacaat caggatcccg
     3061 ttttaatact ctatgaactg tgtcaatact aacatgtaaa gttgcagcaa tatcagctaa
     3121 tgtactccca tttaatctca tttgctttgc aaattgataa cgccgataag catcaatatt
     3181 cttggaatcc atagacaata atacttttct taccatgtat ttcctccaaa gattggattt
     3241 taatgggcca tcatttcttg agcaatctca tgctcagata atgaagctgg atagaaagta
     3301 ggccaattat gcatttcatt ataaagtttt tcttgacttt ctccaccaaa gaaaatatgg
     3361 aaatgctctg ccttacttgg tgcaattcca tgatcgctaa attgaatata tttaaaacga
     3421 ccagcacctg catcatcagt ttcaaaatta aaacgaacac cacggtttcc tttttcataa
     3481 ttcaaaattt tgtatccaac atatttgtat gtgtattgat gatgttcacc gttgactaca
     3541 aaatcaatgg tgttgtcttt aatgttgata gtatcaatat ctgtcttata gccagtagta
     3601 taataatctt tgtactcggc agctgtcatg tctttattga gttttgcttt gtaatcgaaa
     3661 acttcatcca aaattccatc ttttaacaac ggataaacag attgccattc gcctgtataa
     3721 tcactcaatt cgcgatcctt cacatcggca tctttaaagt acccattagc aactgtcttt
     3781 tcatccttcg cttctttttc tggaactacc tcttcaccag caaccatagt tgttttctct
     3841 aacgctttta gattgtcttc cattactgaa acataatctt caccattatc catttgttct
     3901 tttgtaagac tttcaagagg attcaatact tccaatttta tcccagcttc attggccaaa
     3961 gtttttgcga ttttatcatt agcattcttt tcaaagtaaa tataattgat cttatttttt
     4021 ttaacatact cttttaattc agctaatcga ccagaagatg gctcttcttc aggacttagt
     4081 ccagcaatag gaacttgtat caatccataa tccaaagcta aatatccaaa ggctgcatgt
     4141 tgtgtcacaa aacttttttg tttcgcttct ttaagactag tggtatagtc agcatccaac
     4201 cgtttcagtt tagtcaaata cttttcagca tttgtctcaa atacttttgc ttttttcgga
     4261 tacaatttaa ctaactgatc cttgatattt gaaacttcct taattgccat tttgggtgaa
     4321 acccacgtat gaggatctag ttcatgatgg tgatcttctt ctccgtgatc atgatcttct
     4381 tcttcactac ctggcaataa aatcatgtct tttgttcctt ctactacatt aggcccagct
     4441 ttcttccaac tttctttagc ttttggtacc catgattcca tgttttcgtt atgatataca
     4501 aaaacatcag catcatgagc ttttgccata tccttagctg agggctcata gtcatgaggt
     4561 tctgatcccg ctggaaccat taaatccacg ttcgcttcat cgccgacgat gtttttggtg
     4621 aaatcataca ttggatagaa tgttgttaca atttctaatt ttccatcttt atcattcgat
     4681 acttttgcat cattagtgct ggagcaagct cccaataatc ctagagctgc tatcccaata
     4741 agtcctacac tgattttctt cattcttttt ccttccttaa ttatgctaat ttcttcattt
     4801 tattttgttt atgttctttg ataattttta atgccctttt tctagttttg atattcgggc
     4861 ttttaagtcg tcgatatgca ctagatatat ccgttttctc cattagaaat tacccctaat
     4921 caatacttat cattatttgc tttccgccag taatttatca aaactctgtt tgatcctttc
     4981 ttcatccaac atttttccaa ttagaataat agtggtttgg tctttttgtc gagtggtgta
     5041 ttgaaaatta acctgttcgt ttaccccttg tatggcaact ataaaatctt gatcttgcaa
     5101 agcaatcaat cctttaaaac gatacaggct cccttggttg gttaaaatca gccggtcaat
     5161 taattggaat aaaaaatctt tttttatgtc ttgatctgaa gttaagagaa ttgatttaaa
     5221 ctcatgttct tgatgatgtt gatgatagtg tttgccatta tagtgatttt ctgatgaatc
     5281 actttcaaaa actaagggat gattgaattt ttccaaatta taaaaatcct ttttttgaat
     5341 cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg ttaatacttt tgatttctct
     5401 ctgtaatttt tttagtgcag ataaattcaa gttttgtttt ctagacacaa aaatgcgatc
     5461 tgctatgaca agttgcttaa gggcttgtgg ttctctttca ataagttcaa aactgtcact
     5521 atcaataaca gtcaataaac tatcgatata tactcttcct cttagttgag gcgctgttaa
     5581 aagtgtttga atgattggtt ggggatctgc tatacctgta gtttcaatga tgatttgtga
     5641 aattggttgt tgatgttctt ctattgtttc cagtaaagaa atcaatattg ctaataaatc
     5701 actgcgaaga ctgcaacaca tacatccatt atttagttgt aacacccgtt cctcgctatg
     5761 aactaataat ttgtgatcaa tcccaacttc accaaactca ttttcaatta taataatttc
     5821 ttccctgggg tagggtgaga cttgcaaggc ttgattgatt agcgttgttt ttccagcccc
     5881 taaaaaccca gaaacgattg taactggtat gcccattttg tcaccttttt accacttagc
     5941 tatttcactt ctttgaaaat tgcccgtctg cgcaattttg gcgaatactt ttttaattct
     6001 aaccgttcag ggttattccg tttatttttg ctggtgagat acagacgttc tccagtttcg
     6061 acacattcca aaataatatt ttgacgcata aatcttcctc cttcaatact tatcgatctt
     6121 caagatatgc ttgaatttgt tgtgcatctt caaaagttaa ctcgccagat tccaattgtg
     6181 aaactaaatt ttctttcata tcaacaggta tctcccaata accatcaata cattgaaata
     6241 actcctcgtc atactcttca aaaattggat agattcttaa aaaatcatca aatgataagc
     6301 cttggtcctc atagtaatct ttgactaatc cattttcaat tgcctgttgg gtcggtaaac
     6361 ccgtttcagt aataattcct ctctctacca tcgaacgata aaaagtgcat ttatctagcg
     6421 attgctgggt cgtttcaaga taatacttta atccttcttc aactaaaggt gggtaaataa
     6481 tttccatttt gattccctcc taaaagttat atattaaact attttgaagc tcctttaatt
     6541 actttctttc cattatagta cccttttaaa gatacatgat ggctacgtct ataatcacca
     6601 ttcgcttcgt caaatgaaat ttctggtttt actacagttt gatgagttct tcttaaccgt
     6661 ttcttcgcct tagatgtttt tcttgctggt actgccatga tatttcctcc ttttaccaac
     6721 ttgctttttt tactccagga atcaaccctt gatgggcaag ttctctaaac ttaatacggg
     6781 acataccgaa ctttcgcata taccctcttg gacgaccatc cgtctgatca cgaagcctca
     6841 gacgattcgg atttgaatct cttggcaatt tagcgagagc ggtgcgatct cctgcggctt
     6901 ttaattcctg acgtttatct gcgtactttt caatcatcgc catttgtttc tttgctttag
     6961 caattttcga cttttttgcc atataccttt atactccttt tattttgatt tagacatctc
     7021 aaatcgtaat cgttacgttt tgacactgat actttaccaa caagagttgc tcttgtcaac
     7081 aataaataac atgaaataaa actcatatga atagataact atttgaaaac gaatttatcg
     7141 gtacatagag ataatcaaat catttaaaag aatatgcagt ttaagctctg aaaaataaga
     7201 attcactttt attagtccaa tcaaacacaa ataccgtttt aagtagcttt tcctctactt
     7261 aaaacggcaa tatgaaagaa actataactt tttattcaat cttttatgtt taactggttt
     7321 atcgccaatt ttttaaaact ttgattattc cttatattat aagaatagac attaagtgaa
     7381 ctaattcaat catactgatt ctttcgtgta ttcaaaatta gatgctgttt aataaaatta
     7441 ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt taaagcctat
     7501 ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct
     7561 ttatgagatt tttgtatatt tctgaccttc gatacaactc actatgagta ccttgatcaa
     7621 ctatttttcc ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat
     7681 gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt
     7741 tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt
     7801 tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt
     7861 tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag
     7921 ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat
     7981 tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa
     8041 acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata cagccttcat
     8101 cataatcata cagtctagac actaatttca agatactagt tttcccggaa ccactagcac
     8161 ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg
     8221 tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac
     8281 tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt attttaggag
     8341 ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta
     8401 ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt
     8461 gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca
     8521 tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt
     8581 tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt
     8641 ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg
     8701 ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa
     8761 taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa
     8821 acagagccat accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa
     8881 cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg
     8941 gtaaatttga caaatcgtct gcaatttgca ttcttaaatg cgcactttct ttataagtac
     9001 tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacga
     9061 acgatactgt aaaataaagc caaggcggct ttacattttc taaaactaat tcgtctatca
     9121 gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaag ctagaaatag
     9181 atgctttaac caaatcttct gcgccctgat tagttaaagc gaatttgttt ttataaaaac
     9241 ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac
     9301 acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac
     9361 aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat cataataact
     9421 gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca
     9481 atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca
     9541 atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta
     9601 tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca
     9661 aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga
     9721 ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa
     9781 tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct
     9841 gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt
     9901 cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt gtatgaaaag
     9961 ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc atattttagt
    10021 tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata tcttgcttca
    10081 aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat tccacttaaa
    10141 aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga agttataaaa
    10201 aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg aaaaagaaca
    10261 aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc gttaattgct
    10321 ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac ttgaattcct
    10381 cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata tttttgaata
    10441 aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa catggcgatt
    10501 agaaccaatc caattttaaa attaatgaaa aagcctaagg caactgataa aacaggtgtt
    10561 actatggctt gagcactatc tggaatcaag tgagcaattg ccatatgtgt tttagccgcg
    10621 ttgtcatcaa ttgtctttcg aatatatccc gaagaattta aatcaaaaaa acgaaaactt
    10681 gcttctgtta aaccgctaat tccctttttt ctcaaattag tctctaagcg aaacccaagg
    10741 atatgagaaa ataagccaga aaaaatataa aataaagctc ctaaagttaa ccaagcaaca
    10801 gtttgaaccg cataactctt agctaaatat tcatctccta caataattag agacttaaga
    10861 aatttgtaaa tactgtaata tccatagact gttaatacga cagaaaaatc agaaaataaa
    10921 gtggctaacc atccgtagta tttcatttta gggacataac taaataattt tttgtatact
    10981 ttcatttttt cctcccccgc attcaatttc ttctcttatt cgtttacaac tgttaatgct
    11041 ttccgtaata gcgtaaatta gagatgtaca aacagataga aggtaaacta cgttctattt
    11101 tttgtatttt tttaagactt ttcttccaat caatacacca atcatagata aacttgctac
    11161 aactaacact gaaatgattc cccatatacc taccgcatag tggatcattg catccattgt
    11221 tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa agaaccatat
    11281 aggaatccac tgtccggcag cactgcctaa ttgcattaga gcccaagacg caacttgagg
    11341 aattgctcga tcatatccaa atacagatag aataatatca gctaagatcg ccatactaat
    11401 cgaactgata aaccaaggta agtacgcccc gcccattaac gtaaaaaata acaataaaac
    11461 aattgtgtaa atagtaaaaa ttccttttct agggtagtta tagctaatat aaacaaatat
    11521 gattccagat aataatccaa aaatccctgg ggatatgctg tgaccaaacg ccccaccaat
    11581 actactaacc attattgaaa aaaagtaaat tactagggaa ataactgtag ctaaaccaat
    11641 aaaaatataa tcttttgttt tcatatatat tctcctcaat tctatttcgt tatgtcatta
    11701 aaaatctgtt ctatcaaagt tgaatcttct actctaagtt ttatatcttt ttttattcca
    11761 ccatttttta aataaataat tctattgcat gttttataaa gaaattccgg atcatgagtg
    11821 gctataacta ctgcaccctt ttcttttaag tctttaataa ggtcagcaat cctttccata
    11881 ttaatataat ccaacccact tgtaggttca tcaaatagaa ataaattagt ttttgacaat
    11941 gaagctatag aaaccaataa tctttgtttt tctccaccgg ataaatcaaa cggtaattga
    12001 tctttcattt tataaagttc catttttttt aaaatttttt cttgctgatc tttactaact
    12061 atttcattcc ccagggctaa ctcgctcctc actgattctg tgaaaaattg gtaatccatt
    12121 tcttgcatta ctaaaaacgg cgatgacaaa ttttttttat tttcgattat ctttcctttg
    12181 gtagggcgaa taatcttcat gagtattctt agtaaggttg ttttccccac accgtttttc
    12241 cctaccaaac caataacatc tccaactttg acatcaaaat ttatgtcttt tagaatattt
    12301 tcataagtaa tttcttcaac ttttaataca tctgcactta ccttttcgat atagggaact
    12361 tcaatcgaac tattaaaaat gtcaaatttc cttaatgcgt tattgttttc agtttttaac
    12421 aactgaatag ttgtagtttt tacagttcta tttttaacaa atagaacttg atcaattaat
    12481 tctttcaaat agtaaaaacg atgttcagct ataacaatag taaatccttt attttttaaa
    12541 tcacaaagta agtcagcaag tttcttagtc gctttgaagt cc
//
LOCUS       DAGCWU010000073        12412 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.073, whole genome shotgun sequence.
ACCESSION   DAGCWU010000073 DAGCWU010000000
VERSION     DAGCWU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.073"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(12..818)
                     /locus_tag="MBZ71_001593"
     CDS             complement(12..818)
                     /locus_tag="MBZ71_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429552.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            956..2470
                     /locus_tag="MBZ71_001594"
     CDS             956..2470
                     /locus_tag="MBZ71_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBT4429553.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2873..3856)
                     /locus_tag="MBZ71_001595"
     CDS             complement(2873..3856)
                     /locus_tag="MBZ71_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBT4429554.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMKANKNIGKIILKITE"
     gene            complement(3861..4508)
                     /locus_tag="MBZ71_001596"
     CDS             complement(3861..4508)
                     /locus_tag="MBZ71_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4429555.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(4593..5225)
                     /locus_tag="MBZ71_001597"
     CDS             complement(4593..5225)
                     /locus_tag="MBZ71_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4429556.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(5241..5792)
                     /gene="coaC"
                     /locus_tag="MBZ71_001598"
     CDS             complement(5241..5792)
                     /gene="coaC"
                     /locus_tag="MBZ71_001598"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBT4429557.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5789..6526)
                     /locus_tag="MBZ71_001599"
     CDS             complement(5789..6526)
                     /locus_tag="MBZ71_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBT4429558.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNCLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEETQSKSEIAALITEKIRLEESK"
     gene            complement(6523..7512)
                     /locus_tag="MBZ71_001600"
     CDS             complement(6523..7512)
                     /locus_tag="MBZ71_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBT4429559.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7770..8312
                     /locus_tag="MBZ71_001601"
     CDS             7770..8312
                     /locus_tag="MBZ71_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4429560.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8329..9414
                     /locus_tag="MBZ71_001602"
     CDS             8329..9414
                     /locus_tag="MBZ71_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429561.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            9426..10232
                     /locus_tag="MBZ71_001603"
     CDS             9426..10232
                     /locus_tag="MBZ71_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4429562.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            10233..11060
                     /locus_tag="MBZ71_001604"
     CDS             10233..11060
                     /locus_tag="MBZ71_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4429563.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            11057..12130
                     /locus_tag="MBZ71_001605"
     CDS             11057..12130
                     /locus_tag="MBZ71_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4429564.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         3877 a         2241 c         2564 g         3730 t
ORIGIN      
        1 tttagcatgt tttatcgttt gattttttta ggaataaaat tcttgatgaa cgtaaatttt
       61 tggtaatcgt ttcgactata gaggtaacca aatacactga aaacaactgc tccaataaaa
      121 ttaacaaata aatccttcat agtatccatg attcccacgt ctaagtaacc attttggatg
      181 attgttttat ctgttttacc atctgcattt tgactttcaa tgattgtccg agtgatatct
      241 ggaacatgat agacagtgtt tccgtcttta cttaactcca cactattgat ttgctggatg
      301 atcgtatctt tttgcatatc gaattgcagt aatttatctg ctgaaaattc cacaaactcc
      361 cacataacac caactgtcat cgaaaaacag aaggaaacaa ctgctagtaa taaaggagtc
      421 aagttcatgg tttttgtatt tttattgagc aaataaacaa gggaaaagcc gacaccccca
      481 gctaaaaatc cgctgaatgc atgtagaaaa gtatcccaca ttgaaaaata gccataaaaa
      541 tcacttaact ctcctaaaac tgttgaacta aaaataaaag ataacaaaat gatttctagc
      601 atatttggaa tctgtatctt taatatcttt tccagaaaat aagggatttc aaataaaatt
      661 aatgtcagta ataaaagcat tgtatttccc cagtgttggt tccagacctg atccactagc
      721 ataataacga cgatgatacg taataaaata tagattccgt ttgtgattct acctgcagtt
      781 tttgtactgt tcgtaaaaaa ggttttcaaa tgattcataa aataactcct tcatgtatat
      841 ccttaattat actaagaagc gtgtgtttac caaagctata tgacttaaag aaaaacagag
      901 ttgatattgt gaaaaaaatc acttaatata gtataaggag aaaaggggga tacaaatgaa
      961 aaaattagtt gctggtttat taacagccgt tgcacttctt gctatgagcg catgcggcgc
     1021 agatcagaaa aatacagcgc agacaagttc tagtacgaag gaatctgccg aagtatcatc
     1081 aggcgcttct gaacaagtat atacagatcc aaaagaaatg aaagaatctt atgatgtcgt
     1141 gattattgga gctggtggtg cagggatggc agcagctatc gaagcaaaag atgctgggtt
     1201 gaatccagta attttggaaa agatgcctgt tgccggagga aacacatcta aatcatcaag
     1261 cggtatgaat gcttcagaaa caaaatttca aaaagaagaa ggaatcaaag actcgaatga
     1321 tgccttctat gaagaaacgc taaaaggtgg tggaggaacg aatgataaag aaatgcttcg
     1381 atactttgtc gatcactctg ctgatgcggt cgattggcta gatcaaaacg gtatcaaact
     1441 ggacaactta acgatcactg gagggatgag tgaaaagagg acacatcgtc cgtctgatgg
     1501 ctcggctatt ggtggatact tagtggaggg gttattgaat aacgtccatg agcgggaaat
     1561 ccctattttt gtcaacgcag atgtgacaga cattaagaaa aatggggaaa cggtcaatga
     1621 agtgactgta catgttcaag gagaagaacc aaaagaggtc accggaaaag caatcgtagt
     1681 taccacaggc ggttttggtg ccagcaaaga atttattgaa aaatatcgtc ccgatttagc
     1741 tgattatatt acgacaaatc aagaaggatc aactggagat ggtatcacca tgattgaaaa
     1801 attaggtgga caaaccgttg atatggataa aatacaaatt catccaactg tgcagcagga
     1861 tgagggggtc ttgattggtg aggttgttcg cggagaagga gcaatcttgg tcgatcaaga
     1921 ggggaaaaga tttgtcaatg aaatggatac aagggataaa gtatctgcag caattacagc
     1981 attgcctgaa aaatcagctt atttgatttt tgaccaaggc gtgcgtgatc gtgccacagc
     2041 tattgaattt tatgaccaaa aaggatatgt cacaactgga aagacaatgg aagaattagc
     2101 agaaaaaatc gatttaccaa aagaaacact agcaaaaaca gttgatggat ggaatacgga
     2161 tgttgcggag aaaaaagatg ctcaattcaa ccgtgccaca gccatggaac atgacttatc
     2221 gtctccgaat tattatgcga tcaaaatcgc accaggaatc cattacacga tgggcggtgt
     2281 gaaaatcaat acgaacactg aagtattaga taaagaaaac cagccaatta gggggttata
     2341 tgctgctggt gaagtcacag gtggcttaca tggaaacaac cggatcggtg gaaattcagt
     2401 tgcggagatc attatttttg ggcgacaagc gggcatacaa gcagctcgat ttgcttcgga
     2461 aaatgaatga aatagaacgt aaataaaaag aggttgtgaa atcagcgttt tgagctaata
     2521 ccgagggtca gctgattgaa cactgtttag tgaagaggtt gtgaaaaagc tgccctgcat
     2581 caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gttggactta
     2641 ttaccgcaga tgcaagcttt tgaacaccgt ttattcggaa aaagagcgtg ggacaaaagt
     2701 aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagaaattcc
     2761 ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta
     2821 tttcttggag ttaaacgctt ctgtcccagc ctcttttttg atcagcatga ttttattcag
     2881 ttatcttcaa aatgattttt ccgatgtttt tgtttgcttt catatattca tgcgcttgtt
     2941 tgactgcgct gaattggaat gtacggtcaa tgattggccg aatcttgcca ttttctagaa
     3001 aaggcccagc tttttgcata aaagtttgtg cgagctctgc tttatactga tcacttctag
     3061 gtgtcaataa tgtaccaagc agttggacac gtttcccaat caatacacca aggtctatct
     3121 caggcacgat ggtcccacct agcataccaa tcaagaccca acgtccatca acggctatag
     3181 aagctaaatt cttttcccag taagaagcgc cgatgaaatc caaaataaca tcgactcccc
     3241 gaccttcagt gatttttcgt acctcttctt gaaagtcttg ttttttatag ttgatcaatt
     3301 catctgcacc aagttcttta caaaaggcga gtttttcttc tgatcctgca gtaacgatga
     3361 ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc tgttcccaca ccacttgctc
     3421 ctgcatggat caagacagtt tcgccttctt gtaattttcc aagccagtaa agtgtttgat
     3481 aagcagtcaa aaaaacttct gagattccag cagcagaaac atagtctagc gtatcaggga
     3541 gaaggattgc acgatcggca ggcatcacag catattcggc ataaccacct agattgacta
     3601 atccgtatac ttttgtccca ggagtaaaag tggagatatc acttttattc tcaactacct
     3661 ctccagctac ttcgactccc aaaatagggt agggaggttt caaagagact gattctcttt
     3721 tcataatatc tgttcgattg acagctgctg tatggacttt gatcagtaat tcgccttgtt
     3781 ttggtttagg aacaggcact tcaatttgtt ctaatacatc tacagaacca ggatgcttga
     3841 ttgaaatggc acgcatggat ctattcctcc tttgcccatt cttggaatgc tgtattcaac
     3901 tctttgctac cttcagaaag tactagagtc gtacgagaaa tatccgtttc ttccactaga
     3961 gcggcaagta cagcagcaac tgtagagcga gtgatcgttt ttgatgcctt ctcattaggc
     4021 ttagttaatt gaatggaagt aacttcttca tcgttagtca atgtgactgg ttgaagaatg
     4081 acaaaatcta agttagttcg tttcagccat tcatctgcat aatgtttagt aatataataa
     4141 tctgtcatgg actcaggcca ttttgctggt tcatctgcat aaacagcact gaccatcaga
     4201 tagcgagaga catgcgcatt ttcagctgcg atcactgttt tgattgcgcc atccaagtct
     4261 acttgcagaa gattctttcc ttgagagccg gcagcaaaaa tcaatacatc gatgcctttg
     4321 aatgcttcgg tcattttttc cacggaccaa gttaaatcaa aagagacata cgaaacattc
     4381 tcttttttta ctaatgtttg ggaagcaaca tctctcaccc ctgcaaaaat ttcatgggaa
     4441 cttggagcta aatcttttat caggtgtcgg ccaatttgtc cgtttgcacc tacaacaaaa
     4501 attttcattt ggatcatcct ttcgacgtca aatctttcca ttcaatagta acaccaaaag
     4561 agaaaaaaat cagagatcgg ctacgatgac tatcagttag taagagaagg gtgcttgaag
     4621 tatcttaaca agacattgca tactgctgca gtcgcaagac ctgcgacgat tccttcaggt
     4681 ataccacttg taaaaataac cgctagaata gctgaataaa cagcatttaa atttgttccg
     4741 atgactttgg cataatcttg ctggaataaa aagtagatca tattcatcac taaaatcgta
     4801 ttggtcaatg aaccggaaag tccagctaag aataaagaaa cggaatctgc tttttgtttc
     4861 attagtttgc gcagaccttt ataaacgaaa taaggaacca ccccaatcaa gatgcgagga
     4921 ataaacgcga tcagcaaggc tttccagctt ccatgttctg ttccgatgac tgggataaac
     4981 ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc tacggatcat gctgatcagt
     5041 ccaaatgtac cgccaagaaa agcacctagt cttggtccta aaacgatcga agcaatgatt
     5101 acaggaatat gcatggttgt tgcattgatt ggaccgatag ggataaaacc taaaggcgta
     5161 acagctaata aaatcaaaat agctaaaaac atcgcagtta atgtaaagtt ttttgtattt
     5221 ttcataagta ctcctttttt ctaagcattt ctttcattaa gagtcagcat gacctgttcg
     5281 atgatcgtct gaatatcagc taaggcacct tttccaaaat ctccacaagc taaaagagat
     5341 tctcttggtt caatttcctg atagccgaca gatctcaatg tttccagatt acgctgagtg
     5401 atcgggtttt gatacatatt cgtgttcatg gcaggggcaa tcaattttgg tgttcccact
     5461 ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa tgccgtttgc tagcttgccg
     5521 ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag cttgttttgc tagttcgata
     5581 tgattgatta cctgcggatt tgattcttgc atgacgtctg tatggactgc gcgcttcgac
     5641 agagattgca aggttaatgg cgtgataaaa tgtgtgctgc ttttagacat aatcacatct
     5701 acctgggcac ctaatttgac gaattgactt gtaagatcag cgcttttgta agcagaaata
     5761 cttccagtaa cgcctagtaa gatctttttc attttgattc ctccagtcta attttctcag
     5821 taatcaatgc agcaatctca gatttacttt gagtttcttc cactgttcca tctttagata
     5881 agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa atcatttgcg aaaataaaat
     5941 cagttttgtt atttatcaga caattttttg cgactgccag aagagattcc tttggtacat
     6001 ctactaatag tttgaaacca atgatttttg cttttggctg ccattcacgt agataagaaa
     6061 tgatttttgg tgttttcttc aaaacgagca caagatgctc ggtgttagaa gagatctttg
     6121 aaacagtggt atctgtctgt tcattttccg caaaccaatt gtccagttct tcttgcgttg
     6181 aagaagtggg taggtttttt gccagctgtt cttctgaaaa actaaaagca ggagtaaaat
     6241 cactgactgc catactgtgg ataatcgcat cgtagtgttc ttttttcgtt agttcttcta
     6301 attgtaaaaa taagtccagt gcagattcga tcgtataaat cgttatattg gagtgtcttt
     6361 tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt ggtatccgga ttggcagcta
     6421 aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg atttgtaata gcacgtactt
     6481 gatcgatttt ttcagtcgtt cctccagctg tgattagaat cttcatttat ggtcacctac
     6541 atttactagt gttcttcctt ggtgtgttcc agcaagtagt ttagatgctg tttcaggcaa
     6601 ttgatccaga gtaatctctt ggacgaccag ttcatctgca atctgcaaat ccgccatctg
     6661 ttgccataaa gaaagacgtt gatctattgg tacattgaca gaatcaattc caatcaactg
     6721 gatgtttcgc agaataaaag gcaagacagt cgcgtttagt ttgattcccc cagcattgcc
     6781 acataaagct actgcaccgt tataagagat caatggaaga agactgctta gctgctcgcc
     6841 acctactgtg tcgatgacat agtcgatttg ctgttttcct aaagcttttg ttgtctctgg
     6901 cagaaattca tcaggtcgta cgacttgcaa agcacctaga ctttttagcc atgtgacaga
     6961 ctcttctttt ctggaaaagg caatgattga ctggaatcct agtttatgta acagggctat
     7021 cgctgtactg cctacaccgc cagaagcacc agtaacgaca acttttttat cggaggtcat
     7081 accttgacgg attaaagcgt tcacacacaa catagcagta aaccctgctg taccaaggat
     7141 catcgcttga cgcagcgtca tattttttgg tagaggaact aaccagtctc ctggtacttt
     7201 ttgatattgg ctataaccgc ctggatgggt gacgccaagc ccaaaaccag tagcaagtac
     7261 aagatcttct tttgaaaaac gtggattgtt actttggacg acagttccgg ataaatcaat
     7321 ccctggagtc atcggatatg ttcgtatcac ctgaccagac tcgctcattg ccaatgcatc
     7381 tttataattg acatcagaat aggcaatttt taccaagact tcattttcag ccaactcagt
     7441 aaatggcaga tcactgattg ctgtatggaa aacaggttcc tttattacct gaaaagatcg
     7501 aaaagcgttc attcgtgaga cactcctttt aaaattttct tttatcatac cataaagcct
     7561 actcttttat tcttgttctt ccgtaaattt agacaaaaat taggtgaaaa ataaagaatc
     7621 aaacaactag aatttattcc aaaagataaa gaaatattca gcaaaaaaaa ttttacaaag
     7681 ggttgccttt ttcaataaat ttcgtatact aatacgagtg gtagtaaaca ggaagtttac
     7741 tattagaaaa cttcgattcg gaggaaatta tggaaattgg tgagaagtta agaaatttgc
     7801 gcatccaaaa gaatttgaca caagaagaac taggcgaacg cacagatttg tcaaaagggt
     7861 atatttccca gttggaacga gatttaagtt caccatcaat ggaaacattc ttttctattc
     7921 ttgaagtctt aggtgtgact ccagaagaat tttttcatca agaagcagcg aatctgcaag
     7981 tggtttattc aaaagaagat catacggttt atttcgatga agaaaacggg tatgaattgg
     8041 aatggctcgt ggctgattcg aatgaaaaag aaatggagcc tgtcttgctg acttttgaag
     8101 gtgcaggaga atataagacg tttgaacctt cattatctga aacgtttatt tttgtattag
     8161 aaggggaatt agagttaagc ttaggagaga cggtctatgt ggcaaaagca ggacagtcga
     8221 tctattatca agcgacaaaa caacatcaat taaggaatca tctcaaaaaa cgaacaaaag
     8281 tattgattgt tgcaacgcaa tcatatttgt aatatagggg gtaaaaacgt ggggaaacca
     8341 atcatttcat ttgatcaagt agtgaaacgc tatgatgatg aaacagtatt aaaaaaagta
     8401 agttttgaga tcgaacaagg aaaattttat acactgcttg gtccttccgg ctgtggtaaa
     8461 acaacgatcc tacgtattat agctggattc accgaagcta cagaagggga catctatttt
     8521 gaagggaaac ggttgaatga tttgccagct aataagcgac aagtaaatac agtcttccaa
     8581 gattatgcac tttttcctca tatgaatgtt tttgataacg ttgcttttgg attgaagatc
     8641 aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag atgctttgcg tatggtccag
     8701 cttcctggat acgaaaaacg ggagattagt gaaatgtctg gtggtcaaag acagcgtgta
     8761 gcgattgcac gagcaattgt aaatgaaccg aaagtattgc tattagatga accgttatcg
     8821 gcgcttgatt tgaaactacg tacagatatg cagtatgaac tgcgggatct gcaacaacga
     8881 ttaggaatca cgtttatttt tgttactcat gaccaagaag aagcgttggc aatgagtgat
     8941 gagatttttg tcatgaataa agggaaaatc gtccaaagtg gtacacccgt agatatttat
     9001 gatgagccaa tcaatcattt cgttgctgat tttgttgggg agagcaatat cgtggacggt
     9061 gtaatgatcg aagataatct agttgagttt gttggtaagc aatttgaatg tgtcgatggc
     9121 gggatgcgtc taaatgaacc agttgaagtt gttttacgcc cagaggattt gacgatcacg
     9181 acaccagata aaggaaaact agtcgtaacg gtcgatactc agctgtttcg tggtgtccat
     9241 tatgaaatca tctgttatga tgaacagcaa aatgaatgga tggtccattc aacgaaaaaa
     9301 gctaaagaag gaagcaaagt aggattggcc tttgagccag aagatatcca tgtcatgcgt
     9361 ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca gctacgaaga atagggggga
     9421 caagaatgaa aacaatgcgc aggatctatt ccgtccctta tattttgtgg ctcggtttat
     9481 tcgtgatcgc gcctgtatta atgatcattt atcaatcttt ttttgatatg aatgggcaat
     9541 tcacgctgaa taattatcta tcttatttta cgtcggggac atcgctttct atgacgatca
     9601 attctgtatg gtatgcattt ttgatcacgt ttttcacgct gatcatcagt tacccgactg
     9661 cttatttttt aacaaaactg aagcacaagc aattatggct gatgctgatt attttgccta
     9721 catgggtgaa tctgctgctt aaagcttacg catttattgg gattttcagt gtgcacggca
     9781 gcgtgaatca tttctttgat tttatcggga tcgggccgaa acagatatta ttcacagact
     9841 tcagttttct gttcgtggca acgtatatcg agattccatt tatgattatg ccaattttca
     9901 atgcattgga agagatgaat ccttctttga tcagtgctag tagagattta ggcgcgaata
     9961 gctttgagac cttccgacga gtgatttttc cgttatcatt aaatggtgtg aaaagtggtg
    10021 tccaagcagt atttattcct tctctttctt tattcatgct gactcgtctg attggcggaa
    10081 atcgggtgat cactttagga acggctatcg aacagcattt cttggttaca caaaactggg
    10141 ggatgggttc tacaatcggt gtgattttaa tcattgctat gttcatcgtg atgctcctga
    10201 caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa atttaaatgg tcgtatcttt
    10261 atttagggat cgtcttttta ttcttatatg caccgatctt ctatctgatt ttttattcct
    10321 tcaatgaagg aggaacgatg aacagcttta caggatttac ttgggaaaat tatgaagctg
    10381 tttttgaaga tacacgtttg atcattatcg tattgaatac gttcatgctg gctttcttat
    10441 ctgcattgat cgccacgatc atcgggacat ttggggctat gggaatctat tacacgaaaa
    10501 aacgacgtgc acggacagcg ttgttgagca tgaataacat cttattagtc tctcctgatg
    10561 ttattatcgg tgctagtttc ttgattttct ttacactact aggattcgga ttaggttttc
    10621 agagtgtatt gctttcccat attgctttca gcattccaat cgtcgtcttg atggtcttgc
    10681 caaaattgca ggaaatgaac gattcaatgg tcgatgcggc tagagacctt ggtgctaata
    10741 atttccaagt agtcaaaaat atcattttgc cttatctttc tccaggaatc atagctggat
    10801 attttatggc atttacctat tctcttgatg attttgcggt tacatttttc gtcacaggaa
    10861 atggctttac gacgctctca gtagagatct attctcgtgc aagacaagga atcagtttag
    10921 aaatcaatgc gctaagtgcg ttagttttct tgttctccat gatattggtc attggatact
    10981 actttattag taaagaaaac cgtccaaaac ggctgaagaa aaacagaaga gtacgcgagg
    11041 gggtggcacg gctgcgatga aaaaattaca atctctgttt ttaggtattc tcatcattat
    11101 tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca agtggcatga gtggagcaaa
    11161 ggtacttacc atctataact ggggagatta tatcgatcct agtttgatca cgaaatttga
    11221 aaaagaatat ggatacaaag tgaattatga aacatttgat tctaatgaag cgatgttcac
    11281 aaaaatccag caaggtggga caaactacga catcgctata ccaagtgaat atatgatcca
    11341 aaaaatgatc aaagaaaaac tagtacttcc attagatcat tcgaagatca aaggattaaa
    11401 taatattgat ccacgatttt tagacttgga ttttgatcca gataatcagt attcgattcc
    11461 atatttttgg ggaactttag ggatcgtata taacgataaa ttctttgatg caaatcaaat
    11521 taagcattgg gatgatttgt ggaagccgga attaaaagac agtttgatgc tgattgacgg
    11581 tgcacgagaa gttatggggc tttctttgaa tagtttaggc tattctttga atagtaagaa
    11641 tatggcacag ctgacagaag cagcaaataa attgaatcat ttaacaccaa atgtcaaagc
    11701 aatcgtggca gatgagatca aaatgtatat gatcaatgaa gaagcctccg tggcagtgac
    11761 attttctggc gaagctgccg atatgatgtg ggaaaatgaa catttacatt atgtgatccc
    11821 accagaagga tccaatcttt ggtttgataa tatcgttatg ccaaaaaccg tgaaaaataa
    11881 agaaggtgcc tatgatttca tcaactttat gctagaaccg gaaaatgcgg cacaaaatgc
    11941 agagtatatt ggttattcta caccaaatga agaagcaaaa gcgttattgc caaaagacat
    12001 ttcgtcggac gaacaattct atccatctga tgacacgatc tcccatcttg aagtctacaa
    12061 agatctagga tcgaaatacc taggcatcta taatgaccta ttcttagaat tcaagatgta
    12121 taggagataa tggcaaaact cagtaagaat ggggacttat caacgttcta aagagctaaa
    12181 aatcgtcctt tttctatctg agttttccaa tcttctacca aaagcggatg aaacgccgct
    12241 ttttttgtgt tattttattt tttctaccac gtttttctac cagagaaaag tggacaaaac
    12301 cattgtaaaa tagggcccga gacgggctag taaatctaag attgaaaaag ttacagaagg
    12361 ataaagtaat tggaaactat gcaaatatac ttaacccttc tgtaatctat ta
//
LOCUS       DAGCWU010000074        12260 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.074, whole genome shotgun sequence.
ACCESSION   DAGCWU010000074 DAGCWU010000000
VERSION     DAGCWU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.074"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(44..601)
                     /gene="frr"
                     /locus_tag="MBZ71_001606"
     CDS             complement(44..601)
                     /gene="frr"
                     /locus_tag="MBZ71_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBT4429565.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(604..1326)
                     /locus_tag="MBZ71_001607"
     CDS             complement(604..1326)
                     /locus_tag="MBZ71_001607"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBT4429566.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1462..2343)
                     /locus_tag="MBZ71_001608"
     CDS             complement(1462..2343)
                     /locus_tag="MBZ71_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBT4429567.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2442..3224)
                     /gene="rpsB"
                     /locus_tag="MBZ71_001609"
     CDS             complement(2442..3224)
                     /gene="rpsB"
                     /locus_tag="MBZ71_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBT4429568.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3583..4062
                     /locus_tag="MBZ71_001610"
     CDS             3583..4062
                     /locus_tag="MBZ71_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBT4429569.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4279..5370
                     /gene="phnW"
                     /locus_tag="MBZ71_001611"
     CDS             4279..5370
                     /gene="phnW"
                     /locus_tag="MBZ71_001611"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBT4429570.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5363..5491
                     /locus_tag="MBZ71_001612"
     CDS             5363..5491
                     /locus_tag="MBZ71_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBT4429571.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5495..6040
                     /locus_tag="MBZ71_001613"
     CDS             5495..6040
                     /locus_tag="MBZ71_001613"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBT4429572.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6494..8185)
                     /locus_tag="MBZ71_001614"
     CDS             complement(6494..8185)
                     /locus_tag="MBZ71_001614"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBT4429573.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8605..9552)
                     /gene="arcC"
                     /locus_tag="MBZ71_001615"
     CDS             complement(8605..9552)
                     /gene="arcC"
                     /locus_tag="MBZ71_001615"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBT4429574.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9667..10686)
                     /gene="argF"
                     /locus_tag="MBZ71_001616"
     CDS             complement(9667..10686)
                     /gene="argF"
                     /locus_tag="MBZ71_001616"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBT4429575.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10777..12006)
                     /gene="arcA"
                     /locus_tag="MBZ71_001617"
     CDS             complement(10777..12006)
                     /gene="arcA"
                     /locus_tag="MBZ71_001617"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBT4429576.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3361 a         2535 c         2210 g         4154 t
ORIGIN      
        1 cagaggaagc ggaatgatcc gcccctctgt ttttatgatt ttattaaact tccaataatt
       61 ctttttcttt atcagcagcg atcttatcta cttctttgat gctgttatct gtcaatgttt
      121 gtacgtcttt ttctaatccg cgcaaatcat cttcggtaat atcaccattt ttttgttgtt
      181 ttttatattc atcgatcgca tcacgacgga tgttacgaat cgcgattttt gcattttctg
      241 cttctttttt cacatcttta gctaattctt ttctgcgttc ttctgtcagt tgcgggataa
      301 ctaagcgaat gacatttcca tcattggttg gactgattcc gatatcactt gccataatag
      361 ctttttcaac atcttgaaga atgctcttgt caaaaggtgt gatcattaaa acacgtgctt
      421 ctggaatttg gattgatgcc atttggttca atggtgttgg cgcaccgtag tagttcacag
      481 taatacggtc caaaaggcta gcgttcgcac ggcctgcgcg gatttgccca agttcacgtt
      541 gaagattttg tgctgctttt ttcatttttt cttttgcttc tgtcataatt gcatctgcca
      601 tttttatttc ccccttacag ttgttccgat attttcgcct aagattgcac ggcgaatatt
      661 tccgtgttca tttaaattga agacaagcag tggaatatca ttatccatgc ttagtgaact
      721 tgcagttgaa tccatgactt gtaatccttt ggcaataact tccagatgag tcagttcttc
      781 aaattttact gcatttgcat ctactttcgg atcagcagaa taaacaccgt ctacattgtt
      841 tttagccatc aggataacat ctgcgccgat ttctgccgcg cgtaatgctg ctgttgtgtc
      901 tgtcgagaag tacgggttgc cggtaccgcc tgcaaagata acgatccgtc ctttttctaa
      961 atgacgttct gctttacggc gaatatatgg ctctgcgatt tgacgcattt caatcgaagt
     1021 ttgcacgcgt gtcggtacac ccacgttttc caacgtatcc tgtaaagcta aggcattcat
     1081 aactgtagct aacataccca tgtaatcagc ttgagcacgt tccatgccca tttgtgctcc
     1141 gatttgtccg cgccagatat ttccgccgcc cacaacgatt gccatttcaa cgcccagctc
     1201 gtgaacttct ttgatttctt ggatgatttc tttgattact ggtggtttga ttccaaatcc
     1261 tgcgtcccca gctaaagctt ctccactcaa ttttaagact acacgctgat atttcggtgt
     1321 gaccatgttc attcctccaa tattttctat tgccatttta ccatacttag atgatttcga
     1381 cactctaaga ctggtttttc ttgttttgct taaaaaaagg gagcgcacaa accgtgcgtt
     1441 cccgtctgtt attcaacaga attatttttt cacttggctc ataacttctt ctacaaagtt
     1501 atcttcgcgt ttttcgatac cttcgccaac ttcgaaacgt acgaatgttt taacagttgc
     1561 gccttttgat gcaacgtatt tttcaacagt catatcagga tctttaacga atggttggtc
     1621 caccaaagcg atttctgctt tgaatttctt caagcgtcct tctaccattt tttcaacgat
     1681 attcgctggt ttgccttcgt tcaatgcttg ttctgtaaga acagtttttt catgttctaa
     1741 ttcagcttct ggaatttgtg attcgtttac ataacgagga ttgatagctg ctacgtgcat
     1801 agctacgtct cttgcaactg tttcgtcagt tgttccatct agaacagtca atacggcaat
     1861 acgtccgccc atatgcaagt atccaccaaa tgcagcattg tcgtcttttt ctacaacttc
     1921 aaagcgacgg aagctgattt tttctccgat aacttgagtt gcttcgatca agtcagattc
     1981 aattgtacct ttatctgttt tgattttcat tgcagcttcc atgtctgctg gtttgttttc
     2041 agcaacaagt tctgcaattt cttttactaa atcttggaac atttcgtttt tagaaacaaa
     2101 gtctgtttct gagtttactt caacgattgc tgcagtgtta cctttcacag caactgaagc
     2161 caatccttca gctgcaatgc ggtcattttt cttcgcagcc tttgccattc ctttttcacg
     2221 taaaagatca actgcttttt ccatgtcgcc ttctacttca actaatgctt ttttcgcatc
     2281 catcattcct acgccagtca tttcgcgtag ttcttttacc attttagctg taacgtctgc
     2341 catgtttaac tcctcctgtg tgtttttctt tttaaaaaaa gctgcttcaa aggagagtga
     2401 ggcttgtcgc ccagcctttg aaacagctcc atcattcaaa attattctgc tgaagaattg
     2461 tcgccttcaa caacatctac gatttcttcg attgatgttg ctgtgttttc ttctgtgaaa
     2521 tcttcttcaa ctacttgatc ttcaccttgg tttccttcga tgaaagcatc agccattttt
     2581 gaagtaatca atttaacggc acgaattgca tcgtcgtttg aagggattac tacgtcgatt
     2641 tcatctggat cgcagtttgt atcaaccata gctacgattg ggatatttaa tttgtgtgct
     2701 tcttgaacag caatacgttc tttacgtggg tcaacgatgt acattacatc tgggattctt
     2761 ggcatatcag cgataccgcc taagaatttt tcaagacgtt cacgttcttt gttcaaacca
     2821 gcaacttctt ttttaggtag aacttcaaaa gttccatctt cttccattgc attgatttgt
     2881 ttcaaacggc tgatacgttt ttggatagta tcccagtttg tcaatgttcc gcctaaccaa
     2941 cgatggttaa cgaagtattg tcctgcacga gttgcttctt ctttgattgc ttcttgtgct
     3001 tgtttttttg tacctacgaa taatgctacg ccgccttctt cagcaacgct cttcatgtaa
     3061 tcataagctg catctactaa tttaactgtt ttttgcaagt caatgatgta gattccgttt
     3121 ctttctgtga agatatattt cttcattttt ggattccagc gacgagtttg gtgaccaaag
     3181 tgtacgccgg cttctagtaa ttgtttcata gaaattactg ccatgtttgt ttcctccgat
     3241 ttggttttat ttttcctctt cttatcttct accttgccgg caaacttttt caagcaccta
     3301 ccgacaatcc aataagaatg tggatttgct gatgcataaa gcaccctcag ctattataca
     3361 gacaaaagat ccgtatcgca agagcaaata tacttttctg ctcaacattc aatagtttac
     3421 ctgaaagata aaaagaattc aagaaaaaca gtagatttct ctttgtttct ttattatcct
     3481 ttttccataa aataatttta ggtataaaag acgaatatta ttgggcaatc cttctttttt
     3541 ttgttatgat acatgtgaaa tctttcgtaa aggagaaaga aaatgcgcaa agctcaaaga
     3601 catttgctga ttaaacaagt cattgaagaa ttcccgatcc gtacgcaaga agaactatta
     3661 aacaaacttg ggcattatgg cgttaccgcc acgcaggcaa cgatttctcg agatatccgt
     3721 gatctaaaaa tcgtgaaggc acctgatgaa aatggcgttt cccgcttcgt attgtttcgc
     3781 gaaaaaggaa ctcctgagac taggactgag gaagaggatc ggctggttca aatgattgaa
     3841 gacattgtat tgaaagtcga acgagtccac tttttgacga tcgtcaatac cttgccagac
     3901 aatgctcatt tatttgcatc tgttttagat gagatcaaac ctccctctat tgtcagtaca
     3961 attgctggat tcgatacgat catggtcatc tcggaaaatg aggaggacgc aaaacgagta
     4021 gaagaatttc ttcaccatcc aactgaagta tcactttttt aatcagagca cgtattccta
     4081 atatggaatg cgtgtttttt tattttcaat attttcctta ttgaataaac ctttacactc
     4141 tttacaaaaa tacatcattt tttattcaaa tttttcaaca ccttgattga atcacaataa
     4201 tcatcattta tattggatgt taacaagagc agcttcttgc tctttgtgta tttcaaaaaa
     4261 gaaatgaaag gaaatcatat gtataaatta ttaacttctg gaccattgac gacttctgaa
     4321 acagtgaaaa atgctatgct gatcgatcat tgtacttggg atcaagaata caaagaaatt
     4381 acacaatgga taagaaaaaa attattggat cttgctcaag tatcggaagg aatatacaca
     4441 acggtcttaa tgcaaggtag cagctccttc ggggtagaat cggtattaag ttctgtgatc
     4501 aggccagaag atactgtact gatttgttca aacggcgcat atagacaacg aatgatcaac
     4561 atgtgcgaat gtctgaaact gaactatacc gtttatgagg tatcggaaca tcaaatcctc
     4621 caagctcatg tgattacaca gctgattgaa gagaatccgt ctatctccgc actagcaatg
     4681 gtccatagcg agactacttc cggtattttg aataatttgg aagagattgc tgctgtcaca
     4741 caagaaagag atcttttgtt tatagtggac gcgatgtcaa gctttggtgg gataccgatt
     4801 tgtgtagccg aactagggat tgattttttg atttccagct caaacaaatg tattcaaggt
     4861 gttccaggct tttcttttgt acttgctaat aaagcggaac tggaaaaacg aaaaaatgtt
     4921 agccgaagtt tgtcacttga tctatatgac cagtaccgcg tgatggaaaa cgagaatggc
     4981 aaatggcgtt tcacttcccc tacccatacg gttttggctt ttcaacaagc gctgaaggaa
     5041 ctagaggcag aaggaggaat cgaaactcga tattcacgtt atctcgccaa taatcatgtg
     5101 ctacgacaaa aaatgacaga gatggggttc cagcctttca ttcaagaata tcaggggcca
     5161 ttcatcacct cttttcttta ccctaaggac tggcgatttg attttaatca tttttatcag
     5221 tatttaaagc aaaatggatt tgtgatctat cccgggaaaa tcagcgatca tgaatgtttt
     5281 cgtatcggaa atattggcga gatctatccg gaagatattt tggaactgac agcaattatc
     5341 aaagactata aggagaacgt taatgattaa aactgtaatt tttgattggg caggaacaac
     5401 tgtggacttt ggctgtatgg ctcctgtcca cgcttttaga aatgcttttc tagaaaaagg
     5461 aatccaactg actgacaaag aaatccgcta accgatggga aaattaaaat gggatcatat
     5521 ccaacagctt ctttccctcc cttctgtcaa aaaacaatgg atagctattt atggacaact
     5581 acctcaggaa aaagaggtgg aggaattgta tcaacaattt gaacattatc tgtttaaaga
     5641 tttggtcaag cattctacct tgaaacccga cacattggaa acgatcgaac ggttgagaaa
     5701 cgctgggatc aatattggtt ccacgaatgg atttaatgct cctatgatga caatcgtagc
     5761 agaaaccgca aaaaaagaaa ggtattcacc agacttcatg gcaacttttg aagacgtcga
     5821 agggtatggt cgaccgtttc cttatatgat ccataaaaac atgcagtact tcaaaaacaa
     5881 gtcggttgat gaagtaatca aagtcggaga tacggtctca gatatccagg aagggaaaaa
     5941 tgccggagtg ttgacagtag gtgtaattga aggtagttcg ctaatgggga tttcttatga
     6001 tgagtaccaa aaattagaga tggggaaaaa gatcgtttaa aacagtctgt tgctcttaga
     6061 ttccaacaag ccggcgctga tatttgtgta aacaatttaa aagagttaac agaatacatt
     6121 ttgtacgctg acaagaatcc agaatgactt ttatacaaaa taagaggttg tgaaaaagct
     6181 gtcctgtatc aagtaataaa aacagccaag caaaaatagc tcctcatatt tttcgtttgg
     6241 ttaggcttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaaaggga
     6301 gctgtgacac gacttttgtc acagtcccct tatttgcaaa caaacagtgt ttaagagaca
     6361 acctctccac taaacggtgt tcattcagct gactctcggt atttgctcaa aaaacgataa
     6421 aatttgaaaa gctattttct cttttgtttc tctaatactc ggatcagaac gctgatttca
     6481 cattctcttt ttcttacatc ttatctggtg catgaagtcc tagcaaacga aggtcttctt
     6541 tcaataatac agtcactgca taaactagag ctaagcgtgc ttctttttgt tcatcatcag
     6601 ctaaaatctt cgtatgagca tagtatttgt tgaatgcttg tgctaatttg attgcatgtt
     6661 tcgcaatcac tgacggttcg tatttttccc cagcagacaa gaccgtttca gggtattttt
     6721 gaactaattt aaccacttcc cagctggtat cgtcattcaa tgcatagtct gctgtttctg
     6781 aagggacaaa tcctgctttt tccaacaggc tgactgcacg agcatgtgta tattgcacat
     6841 aaggtccagt ttctccttca aatcgaacaa cttcttccag gttaaagtca aatgtattca
     6901 gtcgatcgtt tttcaaatcg tggaaaatga ctgccccaac cccaacttgt ttagctacag
     6961 catctttgtt ttccaaatca gggtttttct cgctgatttg ttctttagca gattggattg
     7021 cttcatttaa tacttcttct aataagacga tctttccttt acgagttgat aattttttac
     7081 caccttgcgt gatcaaacca aatggaatat ggtgcatgtc gtcagaccag tcaaagccca
     7141 tttccttcaa gacagctttc aactgtttaa aatgatagct ttgttcattc cctactacat
     7201 atagagattg tttgaagtcg taagtacgtt tacggtacaa tgcagcagct aagtcacgtg
     7261 taatgtatag tgtcgcaccg tctgacttct taatcaacgc tggattcagg tcataagcag
     7321 ataggtcaac gatctctgca cctttatcct cgttcagcaa atgtttttct tctaaaagtt
     7381 tgacgatttc atccatcttg tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta
     7441 cttccagcag atcatagatt ttattgaatt ctttcatcga ttcatcacgg aaccattgcc
     7501 ataattgaat agcctcttta tcgccttctt ccagacgttt gaaccaagca cgagcttctt
     7561 catttaattc tggttctgtt tcagctactt catggaattg gacgtataaa cgaagcaatt
     7621 cattgattgg ttctgcttta acagcttctt ctgtacccca ttttttgtaa gcgacgatca
     7681 gttttccaaa ctgtgttccc cagtcaccta aatggttgat gcggattggc tgataaccga
     7741 ttttttccat aatgaaaccg atcgaattac cgataactgt cgaacgcaaa tgtcccattg
     7801 agattggttt ggcaatatta ggagaagaca tgtcgattgg tactgttcct tgattcccga
     7861 tgttgctatc cccataatgt tctttttctt tcactacggt ttgcaaaact tttttgctga
     7921 taagttcttt attcatgaaa aagttcaagt aaggtccgac aacttcgatt ttttcaaagt
     7981 tggcagaatc gattttttct gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa
     8041 cttttgctaa tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat tttggatttt
     8101 ctagtaattg ctcgacttgt tccagtgtta aatcatcttt gacagcgtca tagacagctt
     8161 ttgcgacaag atctttatta ttcatcttta ttcctcctaa agtgttccct tgattgttaa
     8221 acaaaaaaaa tctctagcga cggcatactt agtatgccat cactagagac gagatttccc
     8281 gcggtaccac tccaattgtc ctaaaaaagg accagctcca tctgatatcg gtagacgacc
     8341 gtctgtttaa aacagaattc tccaagtgcg cttcgttgta tcttcttcat tcggcttcca
     8401 ccacttccga attcgctagc caagaatgat acaagtactc tcttgttcaa caaatcttag
     8461 tcttattaaa ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt cgtttcataa
     8521 aaaaaaaatc cagatggcct aaaggagaag aaataccacc tggatagcta taaatcattt
     8581 cgtaaatacg aaagacttaa caaattaatc agctacgata atcgttcctt cttcagaagc
     8641 taaaagattc tcgatatttt ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc
     8701 aacaaatgca atagctgctt ctactttcgg aagcatgctt cctggagcga attgcttttc
     8761 atcaatgtat tgtttcattt ctgaaacagt aacagtttct aatttctttt gatctggttt
     8821 ttggtaattg acatacacat tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat
     8881 gatttcggct aatttttctg aagcaaaatc tttatcgatt acggcttctc gtccttctaa
     8941 tccagtagct gtttctacaa caggaatccc accgccacca acagagactg tgataactcc
     9001 ttgatcgacc agcgtttcga tgacacgtgc ttctttaatc gagatgggtt taggggaagc
     9061 gactactttt ctccatccac gtccagcatc ttctttgaat gtcacagtgg agtcagcatt
     9121 catttgttct ttggcttctt cttccgtgta gaatggaccg actggtttac ttggattttt
     9181 gaatgaagga tcattttcat ccacaataac ttgagtgact aaagaaacca catctttatc
     9241 gatccctttt tccttcagta cttcgcccat ggcgttttgt aaccagtaac cgatagatcc
     9301 ttctgtcatt gcaacacatg tatctaaagg cattgccggt gttttctcag aatcagcagc
     9361 ttgctgctga atcaataggt taccgacttg cggaccattt ccgtgggaga tgatcaattc
     9421 atctccttgt tcgataaatt tcactaaata ctttgctgtt tccattaacg cttcttgttg
     9481 ggcttttgca cttgcatccg tagacaagat ggcattgccc cctaaagcga caactacttt
     9541 tcgtttggcc atttcattcg tcctttcttc agttctaccg aattttctag ataacaaaac
     9601 gagccagcgt tttgtgattt tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg
     9661 ttatgcttaa acgcgaggga taaacaagtt acctaatgtt gctgccatga tcgctttgat
     9721 tgagtgcata cgattttctg cttggtcaaa ttgacgagca tatttgctgc ggaagacttc
     9781 atctgttact tccatttcag tgatgccaaa gcgttctttc atttgttcac cgtaaacagt
     9841 gtttgtatca tggaaagctg gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt
     9901 tttttctacc atttccatat tgatttggta aggtttcaac aatttgatac gttcttcgaa
     9961 tttatcttct tcacccattg atacccatac atcagaataa aggacgtctg ctccgtctac
    10021 acctttatca acatcgtcgg taatcatcaa ttgtgcaccg gatttttcag caaagccttc
    10081 tgccattttg acgatttctt cttcaggttg taattctttt ggtgcaacga tacgcatatt
    10141 cgctcctaaa attgcacctg tgactaataa agagttagcc atattgtttc gaccatctcc
    10201 gcagtaagca acagtgatac cttctactgt tccaaagttt tcttggatag ttaaaaagtc
    10261 agcgatcatt tgtgttggat gccattcatc agtcaagcca ttccaaactg gaacaccaga
    10321 gaattctgct agttcctcta ccattttttg actgaatcca cggaattcga ttccgtcaaa
    10381 catacgtcct agtactttag ctgtatcttc tgtggattct tttttaccta attggatatc
    10441 atttgcacct agatattcag gatgtgcacc tagatcgatc gctgcagtag taaatgcaga
    10501 acgagtacgt gtagatgttt tttcaaataa aagcgcaata ttttttcctt ctagatagtg
    10561 atgtgggata ccacgttttt tcaagtcttt caagtgttct gaaaaatcga tcaaatattg
    10621 taattcttct tttgtaaagt ctttttctgc taataagctt cttccttgga atactgattc
    10681 tttcatatta aacattctcc tttagattgt ttttatttaa gattaaagag gaaatcccga
    10741 cggcagctga cagtaagact gccgtcagga aacaaattac ttcaagtctt cacggacaag
    10801 tggttggctc atgcagcgtg gaccaccacg acctcgtgac aattcacttg aattgatttc
    10861 taacactttg atgccatagc tacgaagcaa ttcgttcgat acataatttc ggttataagt
    10921 gactacaaca cctggagcga ttgccaatgt gtttgatcca tcattccatt gttctcgagg
    10981 tgcaacgatc tcatctccat taccagttgg gatcagtaca agatcatcta atccaagagc
    11041 agctttcatc gtttcgtgta agttatctga atgagtaatc ttgatatcat caccatctgg
    11101 ttcgattgta aagacatcca tcttaccgtc tttactttga attgctggat ggatagtgaa
    11161 tttatcgtga tctaccattg taaatacggt atccaaatgc atcattgcac ggttgttcgg
    11221 aattttgatt gccaacactt tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa
    11281 tttttctaaa gctttcgcat tagttcgttg agaaatccca acagctacta ctttatcgct
    11341 taagatcaat tcgtctccgc cttcgatatg atctggattt tcacgatcca accatacttc
    11401 gattccttta tttgcaaatc ttggatgatg tttcaaaata tattctgtaa acatcgattc
    11461 acgacgacgt gcttcaaaag tcatcttatt gactgtcatc ccatttccca ttgatgcgga
    11521 agggtcgcgt gtaaagtaca agtttggcat tgggtccatg taaaatggat agtcatcgtc
    11581 tgctgacaga tcatatagac tgctagaacg tacttcgata tcttttgtac gaaccccagc
    11641 catgatttta tctaccattt cttgcgtttc cattgataga aggaactcat gaagaccctc
    11701 tcttacagca ttcgatgcaa tatgagattc atccaacatt ttattcaaga attgttcttt
    11761 gacattgcct gcgtcaatcg cttctgccgc taatttttcc aagtaaagag tctcgacgcc
    11821 ttcattttgc aatgtcttcg caaagttgtc atgttcttct tgagcaatcg gaagatatgg
    11881 aatatcatcg aacaacagac gatccataat atctggtgtc aaattttcca cttcttgtcc
    11941 aggccgtttc agtaaaactg tctttaattt ccctatttca gagaaaacgt gaataggttt
    12001 atccatgttg tgttgcctcc ttctttttga ttacatgtat aggatatcct ccttttgtaa
    12061 tcactttcat aattttgtct cattcaaaat gagacattcc tcacattaat attcgataat
    12121 taaataatta aacgtttttc tatttttaag cgtaatttat tcatttttta ttctgtatac
    12181 actttcaaaa aaatatttat tcattgaaaa aatttttttg aataaattaa aaaaataaat
    12241 tatattatcg ttgttagcgt
//
LOCUS       DAGCWU010000075        12194 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.075, whole genome shotgun sequence.
ACCESSION   DAGCWU010000075 DAGCWU010000000
VERSION     DAGCWU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.075"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(156..320)
                     /locus_tag="MBZ71_001618"
     CDS             complement(156..320)
                     /locus_tag="MBZ71_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429577.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            615..1943
                     /locus_tag="MBZ71_001619"
     CDS             615..1943
                     /locus_tag="MBZ71_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBT4429578.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2145..2978
                     /locus_tag="MBZ71_001620"
     CDS             2145..2978
                     /locus_tag="MBZ71_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBT4429579.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2994..3731
                     /locus_tag="MBZ71_001621"
     CDS             2994..3731
                     /locus_tag="MBZ71_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBT4429580.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3886..4854)
                     /locus_tag="MBZ71_001622"
     CDS             complement(3886..4854)
                     /locus_tag="MBZ71_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBT4429581.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5086..5919
                     /locus_tag="MBZ71_001623"
     CDS             5086..5919
                     /locus_tag="MBZ71_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBT4429582.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5953..6792
                     /locus_tag="MBZ71_001624"
     CDS             5953..6792
                     /locus_tag="MBZ71_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4429583.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6818..7378
                     /locus_tag="MBZ71_001625"
     CDS             6818..7378
                     /locus_tag="MBZ71_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBT4429584.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7577..9298
                     /locus_tag="MBZ71_001626"
     CDS             7577..9298
                     /locus_tag="MBZ71_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBT4429585.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9463..10605
                     /locus_tag="MBZ71_001627"
     CDS             9463..10605
                     /locus_tag="MBZ71_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBT4429586.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10621..11832
                     /gene="thiI"
                     /locus_tag="MBZ71_001628"
     CDS             10621..11832
                     /gene="thiI"
                     /locus_tag="MBZ71_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBT4429587.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4154 a         2048 c         2533 g         3459 t
ORIGIN      
        1 cgagtcctac tgccggagta aataaagctt atgcaaagaa aaagaaatcc aatcacttgg
       61 gtttcttttt cttttattat tatgatgaat caagctttct gtttttgtca cgtatgttgt
      121 aatctttacg aaagagtaga gttggctact cataatcaac catacttccc atcatcgcta
      181 aatacgaaat aaatcagcat tcctacacca atcaaagcac aagctgcacc aagaaaaggg
      241 atgacattcc caaagttatc tgtattcata agtatgccac cacccacaaa caaaaagccg
      301 ccagcaatca ggtttttcat tttattcatc tcctttctta cgttatagta tttccttctt
      361 tcaatgagta ttttgaaagc tatcttattg tatgatgttc ttctaggcct cttctttatt
      421 atacaggcca ttttcgttat ctcatagaaa tattcctttt tatctgaaaa ctattgtata
      481 ccgtatgaca tttttcttca tgaagttgta agtatattct cattcaaatg aaaatttcat
      541 catagaagag attgtatttc taaattctta agagtaaaat acgaagtata acttggattt
      601 ggaaggatga ttggatgaaa gctattacgc ctgatatgac aacaaaattt aacaaacagt
      661 ttcaagcaaa tatcaaacag caggcattgc agcgggcagt tgtgaagaac ggaattactg
      721 cttctgctga aaatgtttca gcacatgtgg agaatacacc ggtattttct atagatttgg
      781 ctacggggaa agtggctaat caaatgcaat cggggcgttg ctggatgttt gctgcactga
      841 acacattccg tcataagatg ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa
      901 attacacttt tttctgggat aaatatgaaa aggcaaatta tttctatgaa aacatcattg
      961 caacaggaaa agaggcaatc gatagccgga aagtagcttt cttactcgct actcctcaac
     1021 aagatggcgg ccaatgggat atgatcgttt cattattcca aaaatatggc gttgtgccta
     1081 aatcggtgat gccggaaagt gcgaatagtt caaattctcg agatttgaat aactacttga
     1141 acaaattact gcgtaaagat gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat
     1201 tagacgctat tgaagaaaag aaagaagaaa tgctagaaag catttataac ttcttagcta
     1261 tctcattagg aacacctcca aaagaattcg attttgagta ccgcgatgaa gaaaagaact
     1321 atcatcttga ccgaggactg acaccacaaa ttttttatga taaatacatc ggtgtaaaat
     1381 tagatgatta tgttagcgtg atcaatgcac caacaaaaga caaaccattc aatcgttctt
     1441 atacagtaga gatgctagga aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg
     1501 atatggaaac tttcaaaaaa ttagcgattg ctcagcttga agaaggagaa tctgtttggt
     1561 ttggttgtga tgtaggtcaa tcttctacta gaactagtgg catcatggcc ttagatgctt
     1621 attcaatgga tgatctattt gatacggatt ttacaatgac taaagctgaa cgtttagatt
     1681 ttggagaaag tctgatgacc catgcgatgg tattgaccgg tgttgactta gtagacgggc
     1741 aatcaacaaa atggaaagtc gaaaatagct ggggcgaaaa agttggaaag aatggcttct
     1801 ttgttatgag tgatgcctgg atggacgaat atacgtacca aatcgttgta agaaaagatt
     1861 tattgacgaa agagcaatta aatgcgttca atgaagaacc aattgttcta tctccgtggg
     1921 atccaatggg tgctttagca tagacgagta tttcaatgaa gagtatgtag taaagataaa
     1981 aagagctggc ttaagccagc tctttttatc tttaaagtcc attccctgtt ttttatggtc
     2041 cctgtgctat aattctaaat aaaggataag gattggaaat aatcctttta tcattaaaaa
     2101 tatgatgacg aagtagaata aaattgggag taggggggaa agtcatggat atccaaactg
     2161 ttttatctga tctgaaatta aatggtaaag tgattccagt tgttggtggc gatgttaatc
     2221 agacctatcg tataaaaacc gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa
     2281 aaaaaggatt ttttgaagca gaagtagacg gattaaaaga actgtcagca ttcgtacgtg
     2341 taccggatac gtatatgcta ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga
     2401 tcgagccggg aaaaggggat caacgagatc ttgctgcagc tttagctaat ctccatcagc
     2461 aaacagcgcc acaatttggt tttcgtaaag ataactattt gggtacactt gttcagaaaa
     2521 attcttttga agaagattgg tggacttttt tctttaaaga tcggcttgaa tcccagattt
     2581 ctttggcaga agaaacgaat cgatggaatg tacagcgtca agaaaagtat ctgcgattta
     2641 aagaacgtgt attgaaatcg gttgaaccaa aaaagatcac tccacgcctc ttgcacggag
     2701 acctgtggag tggtaatgtg ttctttgacc agcaagggca tccagttttt gtcgacccag
     2761 ctgtttctta tgggaatcgg gaacaagata ttgcaatgag ccagttattc ggtggtttcc
     2821 gaccggaatt tttagatgct tatcaaacta tatttccatt agaaaaggga tggaaagacc
     2881 gactgccgat ttaccagtta tattatcttt tggcacattt gaatatgttt ggagaaagtt
     2941 acggttccca agtagatcaa ttactagaaa atttttaaga ggagggcgtg tttttggcaa
     3001 aaaaatatac caaaaagcat gaagtttcat attatgagtg tgacatcaac caaacgatga
     3061 catttccttc gatgttagga gtagttatca aaacgtctga agatcaaagc gaccagttgg
     3121 gaagaggcag tgaattcgtc aatagctttg gacttacttg ggtcatcacg aattattcca
     3181 tggagattaa tcgtctccct agagtcggtg aagtaatctc agtaacgact caagccatgg
     3241 agtacaataa gtttttctgt tatcggaact tctggatcca tgatgaagat gggaaagaat
     3301 tagtcaaaat agaatctgtt ttcgtattga tggattttgt gaatcgaaaa atgagcagtg
     3361 ttaatgaaga gatcattgct ccttttgaga gtgagaagat caagaaaatc aagcgtcagg
     3421 aaaaaattga aaagattgat tcgggcttca tgttgccata tcgtgttcgt ttttatgata
     3481 tcgatagtaa tcagcatgtt aataatgcga tgtattttaa ttggatcatt gacgtattag
     3541 gatatgactt tttgactacg catgttccag aaaaagtaat tattcgattt gataaagaag
     3601 tagagtacgg gaatgagatc gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca
     3661 aaacaaaaca tgaaatccga atgcaagaac agctgtattg tgaagcaaat attatatgga
     3721 aaaataaata aaaaagaggt tgtgacagaa gggggcaact tcaagaaata agaagaaatt
     3781 tacgaaaatt gtttctcaaa tttttgtaaa ttttcgactt agttttcgaa gaagctattt
     3841 ttgtcacagc ctctcttttt aagtttagct attagataat gatgtttagt tttggataaa
     3901 cgaaatcaat tcttcacccg acaaaccata gtttacagca atcaatagtt ttaaacgtgc
     3961 tttttgacta ttaatactgt tacagaaaat aatccccatt ttttctagtt cttttccccc
     4021 gccttcataa tcataaacag gctctgcgat tccattgaaa caacgagaaa caagtactac
     4081 agggattttt tggacaagaa gcttttccaa agcagctaag gcttgtggag ggagattccc
     4141 cgcacctaaa gcttcgatta ccaaaccgtc tactttcgta tgggcgatgg cttccaataa
     4201 gtccccttgc atcccagcat atgctttaac gatcgggata gtcccatcta cagcggaaat
     4261 gtccagtcgc tttgtttcta aaagttcctg caagaataaa attcgattct tagttactaa
     4321 gccgatggga cccaatgtag gtgttcggaa agtagcaaca tttgtggtat gggtctttgt
     4381 cacgtagcgt gcagaatgga tctcatcgtt catgaccacc aaaaccccct tatctagcgc
     4441 ttcttcacaa cttgcgacac gaatcgcact ttcaaaatta taaaggccgt cacttccaag
     4501 ttcattactt gaacgcattg ccccagtcaa aacaattggt aatttgttgc caatcgttgt
     4561 atcaagaaaa aaagcagttt cttctaatgt atctgtaccg tgtgtgatca ccactccgga
     4621 tatacctgat gcataagcgg acaagatacg ttcttttaat accaacatgt gagaaggcat
     4681 gatgtgtggt gagggaagat tgaagatatc ttcaactaat aattctacat gcttggggat
     4741 tgtgatttct gcatctaata atgggtttgc cacatcagga ctaacaccgc ccgtgagcat
     4801 gttttctttc atggcgatcg ttccgccggt gtgtagtaca agtatttttt tcattttaca
     4861 gacttcctta cgaataattt tgcatagccg ttaaatctta ttgtatacta tataaaagag
     4921 ttttgctatc agtgaagaat aacgaatagc ctaacttatc ataaaaaagc tgaccttatt
     4981 aaaatggaaa agactcaacc ttgacctcct aatacaatgg agtatctggt tgagtttctt
     5041 ttacaagaaa gcagccaagg aaatcaatca cgaaggtggc cggaaatgat cagaatcgga
     5101 ttaacatcgt ttaaagaaca tgacaaatta actggtaaaa agaataacac tctttatgaa
     5161 tatgcagggt ttctaccctt agtggaatta gatactgcct attatggaat tccccgaaag
     5221 tcctcagttg agaattggat aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc
     5281 tatcaaggaa tcagcggtca aggagactgg aagccttatt atcagaccga agaagagatg
     5341 attgcgcatt ttttagatgt tatgtcgcca atggtagatg ctggaaagat gttttgcttt
     5401 ttagtccaat tcccctcatc attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa
     5461 cttagaaagt ggtttgaggg ttatcctgtt tctatagaat tgcgggatta ttcttggtac
     5521 agcgatgaat ttcttgaacg tacaagaaac tttatgtcta gagccgcttt ttctttagcc
     5581 atcatcgatg agccacagtt gctgaataca acgattcctt ttgatccatt tgtcaccaat
     5641 ccgaactttg ctctttttcg attccatggt aggaatgcgc aaggatggca agcaaatgat
     5701 aaagagtggc gtaaaaaaag aacgttatat cgttattcag aaaaagaact gcaagaacta
     5761 gcacatgaag tcaaagtagt tgattctaag acaaaagagg taggtgtgat ttttaataat
     5821 aattctggtg gtgatgcggc tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag
     5881 ccagatggac tcaatccaac ccaaatggat ctgttttaag ggcttatttt attcaagaaa
     5941 ggggcgaagt ctatgatcaa agctattttt tttgatattg acggaacatt ggtcaccaat
     6001 cgttcaaaag cgttggaatc aacaaaacaa gcaattgaat atgctcggaa aaacggcatt
     6061 ttatgcggtg tagcaacggg gcgcagccca gtaaagatca aagaaatcat tgatgaattg
     6121 gaattagata tgtatgtcgt gtataatggt caactggttt ttactgcaga tcgcacgatt
     6181 atcgaccacc cttttgaaca aaaagtattg gaacatattg ttgaatttgc agatgagaac
     6241 catcgacaaa tcgtttttgg tgcgagaaat cggttagatg gatcaacgac gatgctatta
     6301 ggacaatcga tttttatcaa gcgactagtc agctttttac cgcgtaaatt cccggtacgt
     6361 ctgatgaaaa aaatcctcca ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg
     6421 ttagagatat taaaagaacc aatttatcaa tgtatattgc tcagtccgga gtcagaacag
     6481 aaaaagttag aacatcgctt tcccgagtgt acgtttcagc gttcaaatac gtatactgta
     6541 gatatcatcc caaaaggtgg ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc
     6601 gaaattgaaa tggaagaaat tatggcattc ggcgatcatt acaatgatat cgagatgctg
     6661 aaaggtgtag gaattggagt ggccatgggg aatgctcaga tcgaagtaaa acatgctgca
     6721 gactttgtga ctcaatcaaa tgaagaagat gggatatacg aaggactgaa acacttcggt
     6781 ctgatttatt agtcaatgaa agaaggaaaa aatcagtatg gaagcagaga caccttttca
     6841 aaaagtagaa acattccaca aaacttttga tccaagaaga ccagagatac ccacggcttt
     6901 tacagaaaat gaagctcata cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt
     6961 tgcagctact tcacctaata aagaacaact gcatgaagct gttatgctgc tgcacgaagc
     7021 attagataaa gcagaagaaa aaatacttgg taaaacacag tggggaagtg atccgctggt
     7081 tgaacaagta gatgcactga cagatatcct ttactttact tatggttctt tttcattact
     7141 aggaatagtg cctgatcgaa tattcgaaat cgtccatcag gcaaatatgg ggaaattatt
     7201 tccagatgga aaacctcatt atgaccctgt aacaaataaa gtgttgaaac ctgaaaactg
     7261 gcagatggaa tatgctccag aacctaaaat cgcggcagaa atccaacgtc agattcaaga
     7321 agcgattgct aagaagaaat ccgctgatat aggcatgtgc acaaatgaaa aagagtgatc
     7381 agagtttcac tatttttgtt atctttatac gatggaaaaa caaaagattt tcgtttaagt
     7441 tagtgaatgc atgaaaatag tatgctataa tctagaatag atgtcttttg cacgagagac
     7501 gtattatttg gatttatgcc aagcagaacg ccatctgttg cataaaagat gcatactgaa
     7561 aacggaggaa ccataaatgg gaaataatgt tatcatcgga atcgtcattg caatcattat
     7621 agtcgccatt gtcgtctttt tggttagtta cttgatgaag aaaaaaaacc aagatcgatt
     7681 ggatgcctta gaaaaacgca aggaagaatt atttgacttg ccagtgattg aagaagtcga
     7741 tgatgttaaa aaaatgcatc tggttgggca aagccaaaat acctttcgtg aatggagtca
     7801 aaaatggaat gacatatcta cttcctcgtt cgctgaacta gaaagtcaaa ttttcgaagt
     7861 ggaaaacctc aatgaaagtt ttcggttctt aaaaggaaaa aaagcagttg ttgaagcaga
     7921 agctacaatg gaccaaatgg agaaacaagt gacggaaatc cgtgaaggtc taaaggaatt
     7981 gcgggaaacg gaagaacgca actcactagc tgttcagcag gcacttgacg tctatgaaga
     8041 actgaaagaa aaagtcagag aagacgagca tgaatacgga ccagctcttt ttgaattgca
     8101 aaaacagatc aaagtgatcg aaaaagaatt tacgcagttt gtgactttaa acacttctgg
     8161 agaccctgtt gaagcccgcg aggttttgga taaagcagaa aaacatacgt atgaagtgga
     8221 agctctgatg aaaaaaattc caccgctgta tgaggagctc cataccactt tccctgaaca
     8281 acttgaagaa atcagtgata cttacgagaa attgatggaa gaacaatatg tattcccaga
     8341 agaagatctt gctgaagata tcgcaaaagt aagtcgaaga attgaaaatt cattagccaa
     8401 tcttgaaaaa acagaagttg aaacggtcga atttgaaaac cgtgaaacag ctgatttgat
     8461 tgattcgtta tatgatatct tagaacggga aatggaagct cagagatatg taaaaactaa
     8521 ccaatcaacg attgcagagt atatcaaaca tacaacaaag aataaccgtc aattattgat
     8581 tgagctggat cacacatctc aaagctatac attgaaccac aatgaaattg gccgtgtacg
     8641 aggtttccaa accgaagtgg aagaaatgga acgtcaaaat gaacagatga ttccacagat
     8701 ccgtcaacac gaaattcctt attcagaagt gcgcacgttc tataaaacag tcttcaaagt
     8761 cttagaagat atcgaaaagc aacaagtaga gatcgacgac tcattacatg aattgagaaa
     8821 aggcgaaaaa gaagcgcaag aaaagatcga tacattcgaa ttcaaattgc gcagtttgaa
     8881 acgttttgta gaaaaacaac gtttgcctgg cttgccgaat gattacctag aattcttttt
     8941 tgttgctact gaccgtattg aagaactaag tgttgtcttg aataaaattc gagtaaacat
     9001 ggaagaggtc aatcgtttag ttgctctatg tgaagaagat ctagaattat tagataagaa
     9061 gacacatgat ttagtagatg cagcagcatt aacggagcaa atgctccaat atgcaaatcg
     9121 ttatcgtcat acacacgaag aagtgagaga agcaattgat aaaagctact atttattcaa
     9181 taaagaatat cactatcaag aagcattaga cgaaatcggt acagcattag agcgagtaga
     9241 acctggtgct ttcaaacgca tcgaagattt ttattttaat catccagact tagtataaag
     9301 caaaaaagtt agtaaaccag caagagcaag acggattatt gtcttgttct tgtctttttt
     9361 tgtatatttg aaaattgaaa caagtgaaaa tttcacctgc tcttgaaagc accttggaca
     9421 accgttataa tagaaaagaa tttcaaaaaa gaggggtttc ttatgatata ttttgataac
     9481 agtgcaacaa ccccaatctt tccgcaaagc ttagatgcat atgtaaaaac cagtcagcgg
     9541 attattggga atccatcaag cttgcatgat ttaggcaatc aagctaaccg gttgttgcag
     9601 caagcaagaa agcagatcgc tgatttgatc catgtagaac cagaagaaat ctattttact
     9661 tcaggcggaa cagaaggaga taactgggta ttaaaaggaa cgatgatcga aaaaagagaa
     9721 tatgggaatc atatgatcat ttcaggagtc gaacatccgg ccgtcagcga gacagcagaa
     9781 caattaaaag aattaggaat cgaagtaagt atcgccccag tggataaacg cggatttgta
     9841 cgtgtcgacg aattgaaaga attaataaga aaagagacag tattagtctc tgttatggct
     9901 gtgaataatg aagtaggatc gattcagccc atccaggaaa tcagtgatct tttggatgat
     9961 tttcctaaaa ttcatttcca tgtagatgct gttcaggcga ttggaaaagt accattctcg
    10021 caatggttga cagaaagagt tgattttgca acattttcag ctcataaatt ccatggccca
    10081 cggggaactg gtttcattta ttggaaaaag ggtcgtcgtt tagcaccatt actgacaggt
    10141 ggagggcaag aaagaaatca gcgaagtggg acagaaaatg ttccaggtat tgtggctatg
    10201 gttaaagctt tacgtttatc attagagata aaggaacaaa aacctgagca gacacaacgc
    10261 ttaaaagtag ctctaatcaa tgcgttgaag acgtatgaga aagtgacgat tttttcagaa
    10321 ggaactgact atgcaccgca tattttatgt tttgctctaa aaggaattcg cggcgaagtt
    10381 ctcgtccatg ccttagaaga aaaacaaata tttgtttcta ctactagtgc ctgctcctca
    10441 cgtaaacata tggcaagcag tacattgcac gcaatgcatg taccaaatga tctggctaca
    10501 tctgctgttc gaatcagctt agatgaaaac aattcgcttg cagaagtcga gcaatttatg
    10561 attattttca agcagcttta taagaaattt tctgcaatca attaaaagag gagtgttctt
    10621 gtgaaataca gtgaaattat ggtccgttat ggggaacttt cgacaaaagg aaagaatcga
    10681 aaaagcttta tcatgcaact agcacaaaat gtcagacaag ctttgaaaga ctttccagaa
    10741 atcaaaatcc atgctgatcg ggaccggatg catcttttat tgaatggcgc agacgggcag
    10801 ctagttattc caaaactagc aaaaatattt gggatccaaa atttctctcc aagtattcgt
    10861 gtagaaaaag atgtcgaagt tttgaaacaa gctgttcaag acatcatgaa agaaatttat
    10921 actgggaaag aaacatttaa aattatcgct aaaagaagcg atcaccaatt tgaattgaca
    10981 agtaatgaat tgaatcaaac attaggtaat gctgtttttg atattttccc gcatatccaa
    11041 gttcaaatgc gtcaaccaga tattccatta cgtgtagaga ttcggcgtga tggcgcttat
    11101 ctatcgtatg aaacgatcaa gggagctgga ggattgcccg taggaacaag cggaaaagga
    11161 atgcttatgc tttctggggg gattgattct cctgtagcag ggtactttgc catgaagcgg
    11221 ggagtggaaa ttgaagctgt tcattttgct agtccgccgt atacaagtga acaagcactg
    11281 caaaaagcaa aagatctaac agccaaacta gcaccttatg tgggaacgat ccattttatc
    11341 gaagtgccat ttaccgagat tcaagaagaa atcaaaaagt cagtgccaca aggatattgg
    11401 atgacaatta ctcgtcgaat gatgttgcgt ttgaccgatg aaattcgccg tattcgtcat
    11461 ggtttagtca ttttgaatgg tgaatcatta ggtcaagtgg cttcacaaac tttgcaaagt
    11521 atggtggcta tcaatgaagt aacgaatacg ccaatcattc ggcctgtagc gacaatggat
    11581 aaacttgaaa tcattgaagt agcagaacag atcgatacat ttaatctttc tatccagcct
    11641 tttgaagatt gctgtacgat ttttgctcct ccacagccaa aaacacgccc tcaactggaa
    11701 aaggtccatc aatatgaaga gcgtttagca atagatgaaa tgatccaacg agcactagct
    11761 ggcttgaaga tggaagagat acaagcagaa tcgtctaatg aaaaaactga agaattttca
    11821 gaattgctat aagaggttat gaaaatagtg ttttgaccag agtattagag aaacaaaacg
    11881 aaaaaatagc tccatgtatt ttaccgtttt tgaactaatg tcgaagatta gctgattgaa
    11941 taccatttat caagaagtta tggaaaagcc ggttcttttt tactaactga aaaataaaat
    12001 tttatttaga gatcaagcta cagttgataa atgatataaa aaagagaatt tcccatgaaa
    12061 tagcgaggtt gtgacagaag tgggcagctt caagaaataa gaaggaattt acgaaaattg
    12121 cttttcaaat ttttgtgaaa tttcggctta tttttcgaag aagttacttc tggaacaccg
    12181 tgtatcagag gttg
//
LOCUS       DAGCWU010000076        11997 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.076, whole genome shotgun sequence.
ACCESSION   DAGCWU010000076 DAGCWU010000000
VERSION     DAGCWU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.076"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(56..643)
                     /locus_tag="MBZ71_001629"
     CDS             complement(56..643)
                     /locus_tag="MBZ71_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429588.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            818..2140
                     /locus_tag="MBZ71_001630"
     CDS             818..2140
                     /locus_tag="MBZ71_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4429589.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2295..3071
                     /locus_tag="MBZ71_001631"
     CDS             2295..3071
                     /locus_tag="MBZ71_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBT4429590.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3064..3633
                     /gene="rnmV"
                     /locus_tag="MBZ71_001632"
     CDS             3064..3633
                     /gene="rnmV"
                     /locus_tag="MBZ71_001632"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBT4429591.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3626..4510
                     /gene="rsmA"
                     /locus_tag="MBZ71_001633"
     CDS             3626..4510
                     /gene="rsmA"
                     /locus_tag="MBZ71_001633"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBT4429592.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            4961..5590
                     /locus_tag="MBZ71_001634"
     CDS             4961..5590
                     /locus_tag="MBZ71_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429593.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5714..6823)
                     /gene="ugpC"
                     /locus_tag="MBZ71_001635"
     CDS             complement(5714..6823)
                     /gene="ugpC"
                     /locus_tag="MBZ71_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBT4429594.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7032..7454
                     /gene="mgsA"
                     /locus_tag="MBZ71_001636"
     CDS             7032..7454
                     /gene="mgsA"
                     /locus_tag="MBZ71_001636"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBT4429595.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7777..8313
                     /locus_tag="MBZ71_001637"
     CDS             7777..8313
                     /locus_tag="MBZ71_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBT4429596.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8353..10116)
                     /locus_tag="MBZ71_001638"
     CDS             complement(8353..10116)
                     /locus_tag="MBZ71_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429597.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10119..11834)
                     /locus_tag="MBZ71_001639"
     CDS             complement(10119..11834)
                     /locus_tag="MBZ71_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429598.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3742 a         2218 c         2449 g         3588 t
ORIGIN      
        1 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
       61 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
      121 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
      181 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
      241 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
      301 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
      361 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
      421 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
      481 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
      541 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
      601 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
      661 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
      721 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
      781 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
      841 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
      901 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
      961 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
     1021 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
     1081 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
     1141 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
     1201 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
     1261 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
     1321 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
     1381 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
     1441 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
     1501 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
     1561 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
     1621 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
     1681 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
     1741 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
     1801 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
     1861 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
     1921 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
     1981 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
     2041 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
     2101 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
     2161 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
     2221 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
     2281 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
     2341 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
     2401 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
     2461 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
     2521 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
     2581 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
     2641 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
     2701 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
     2761 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
     2821 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
     2881 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
     2941 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
     3001 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
     3061 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
     3121 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
     3181 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
     3241 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
     3301 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
     3361 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
     3421 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
     3481 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
     3541 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
     3601 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
     3661 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
     3721 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
     3781 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
     3841 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
     3901 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
     3961 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
     4021 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
     4081 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
     4141 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
     4201 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
     4261 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
     4321 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
     4381 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
     4441 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
     4501 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
     4561 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
     4621 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
     4681 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
     4741 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
     4801 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
     4861 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
     4921 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
     4981 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
     5041 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
     5101 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
     5161 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
     5221 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
     5281 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
     5341 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
     5401 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
     5461 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
     5521 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
     5581 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
     5641 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
     5701 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
     5761 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
     5821 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
     5881 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
     5941 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
     6001 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
     6061 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
     6121 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
     6181 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
     6241 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
     6301 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
     6361 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
     6421 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
     6481 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
     6541 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
     6601 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
     6661 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
     6721 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
     6781 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
     6841 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
     6901 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
     6961 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
     7021 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
     7081 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
     7141 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
     7201 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
     7261 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
     7321 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
     7381 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
     7441 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
     7501 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
     7561 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
     7621 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
     7681 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
     7741 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
     7801 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
     7861 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
     7921 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
     7981 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
     8041 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
     8101 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
     8161 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
     8221 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
     8281 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
     8341 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
     8401 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
     8461 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
     8521 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
     8581 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
     8641 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
     8701 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
     8761 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
     8821 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
     8881 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
     8941 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
     9001 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
     9061 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
     9121 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
     9181 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
     9241 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
     9301 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
     9361 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
     9421 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
     9481 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
     9541 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
     9601 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
     9661 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
     9721 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
     9781 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
     9841 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
     9901 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
     9961 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    10021 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    10081 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    10141 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    10201 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    10261 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    10321 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    10381 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    10441 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    10501 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    10561 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    10621 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    10681 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    10741 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    10801 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    10861 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    10921 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    10981 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    11041 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    11101 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    11161 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    11221 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    11281 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    11341 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    11401 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    11461 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    11521 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    11581 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    11641 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    11701 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    11761 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    11821 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    11881 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    11941 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaag
//
LOCUS       DAGCWU010000077        11990 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.077, whole genome shotgun sequence.
ACCESSION   DAGCWU010000077 DAGCWU010000000
VERSION     DAGCWU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.077"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="MBZ71_001640"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="MBZ71_001640"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBT4429599.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="MBZ71_001641"
     CDS             951..1256
                     /locus_tag="MBZ71_001641"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBT4429600.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="MBZ71_001642"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="MBZ71_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBT4429601.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="MBZ71_001643"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="MBZ71_001643"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBT4429602.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="MBZ71_001644"
     CDS             4416..5354
                     /locus_tag="MBZ71_001644"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBT4429603.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="MBZ71_001645"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="MBZ71_001645"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBT4429604.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="MBZ71_001646"
     CDS             6725..7465
                     /locus_tag="MBZ71_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBT4429605.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="MBZ71_001647"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="MBZ71_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBT4429606.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="MBZ71_001648"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="MBZ71_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBT4429607.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="MBZ71_001649"
     CDS             10608..11258
                     /locus_tag="MBZ71_001649"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBT4429608.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="MBZ71_001650"
     CDS             11259..11897
                     /locus_tag="MBZ71_001650"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBT4429609.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
BASE COUNT         4007 a         2241 c         2647 g         3095 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagagggagc aacttacctt
//
LOCUS       DAGCWU010000078        11979 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.078, whole genome shotgun sequence.
ACCESSION   DAGCWU010000078 DAGCWU010000000
VERSION     DAGCWU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.078"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..282)
                     /locus_tag="MBZ71_001651"
     CDS             complement(<1..282)
                     /locus_tag="MBZ71_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBT4429610.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQT"
     gene            complement(288..1151)
                     /locus_tag="MBZ71_001652"
     CDS             complement(288..1151)
                     /locus_tag="MBZ71_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4429611.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(1165..2061)
                     /locus_tag="MBZ71_001653"
     CDS             complement(1165..2061)
                     /locus_tag="MBZ71_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4429612.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(2236..3525)
                     /locus_tag="MBZ71_001654"
     CDS             complement(2236..3525)
                     /locus_tag="MBZ71_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4429613.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(3497..5014)
                     /locus_tag="MBZ71_001655"
     CDS             complement(3497..5014)
                     /locus_tag="MBZ71_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBT4429614.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5212..6102
                     /locus_tag="MBZ71_001656"
     CDS             5212..6102
                     /locus_tag="MBZ71_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBT4429615.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(6175..6999)
                     /gene="nadE"
                     /locus_tag="MBZ71_001657"
     CDS             complement(6175..6999)
                     /gene="nadE"
                     /locus_tag="MBZ71_001657"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBT4429616.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(7011..8483)
                     /locus_tag="MBZ71_001658"
     CDS             complement(7011..8483)
                     /locus_tag="MBZ71_001658"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBT4429617.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(9120..9482)
                     /locus_tag="MBZ71_001659"
     CDS             complement(9120..9482)
                     /locus_tag="MBZ71_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBT4429618.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            9705..10457
                     /locus_tag="MBZ71_001660"
     CDS             9705..10457
                     /locus_tag="MBZ71_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4429619.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(10597..11520)
                     /locus_tag="MBZ71_001661"
     CDS             complement(10597..11520)
                     /locus_tag="MBZ71_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4429620.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(11535..11807)
                     /locus_tag="MBZ71_001662"
     CDS             complement(11535..11807)
                     /locus_tag="MBZ71_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429621.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         3755 a         2420 c         1961 g         3843 t
ORIGIN      
        1 tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta acagactaag
       61 gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa tgatatttcc
      121 tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag caggcacgat
      181 agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt aatcagaaac
      241 cacaatcaac tgtccataaa gtttagtctc aactattttc atcttcctca ctctttcatt
      301 ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac cactagtaat
      361 ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat gttctgccct
      421 gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt caatatcaat
      481 ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga taaaagagta
      541 ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa tccatcaatg
      601 cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat catgaagata
      661 ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag catgccaagc
      721 aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat cattgtcagc
      781 aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa tgcatacccc
      841 gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat cactgtattt
      901 ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc tagggtatag
      961 ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt aaaagaagaa
     1021 ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat actaatcgtt
     1081 cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt aatcgagctt
     1141 tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg acatgtacac
     1201 attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca gtgctgatgc
     1261 atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa cagctgtttc
     1321 tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca cttgcatcga
     1381 accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag gtagcgtaat
     1441 gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat acaaagaggg
     1501 agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat tcttccaaac
     1561 agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt gagggatcgg
     1621 caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa gcaacataga
     1681 aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg tcgaacgaca
     1741 aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac caacgataat
     1801 ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat taactgctcg
     1861 ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg atgtaaagaa
     1921 aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga cagtaaataa
     1981 taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag gacgattcca
     2041 atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa aatatacaat
     2101 cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt tgcaataaca
     2161 cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac agtctatcct
     2221 gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg catcgttcat
     2281 gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga tgatatcgtt
     2341 attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt ccatttgttt
     2401 cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta ctgaatccaa
     2461 gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg tattccaata
     2521 tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg gaatggcata
     2581 tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg gtaaggtagt
     2641 cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc cattgatttc
     2701 aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa tcgattctgg
     2761 accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa ggttctcagg
     2821 agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat ttgctaacat
     2881 ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata catttttact
     2941 actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag gtggttgttc
     3001 agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca tgccttgtac
     3061 ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag ccgtttcttt
     3121 aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt gtaccgctcc
     3181 gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag gtgctttttt
     3241 agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga tatccatttc
     3301 aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt gcaatatttc
     3361 accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat cagaatcaga
     3421 ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta ccaacaaaga
     3481 gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat aaacgacatc
     3541 tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct tcagctttta
     3601 catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt gcttttgtat
     3661 caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca gacaagtgaa
     3721 cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt tctttgtcat
     3781 ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc gcatagtttg
     3841 ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct tgcataaatg
     3901 gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt ggtgcaataa
     3961 catttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc aataaagtga
     4021 ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct agtaaaggag
     4081 gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac caaatattcc
     4141 attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg actgtatcgc
     4201 acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca agataatgag
     4261 aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct atttgatcgt
     4321 acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc cgattcgaac
     4381 ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta gccgtttcct
     4441 ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga ccgtccattt
     4501 cattccctaa acaccatgtc ttaattccaa aagggctctc ttgaccattc gaacgcctta
     4561 aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta cgagctgcat
     4621 ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca tgctgacacc
     4681 agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa gctaaatcca
     4741 accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat cctgacacaa
     4801 aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag tgaatgacgt
     4861 cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc ccttcatata
     4921 ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact ttgcctacac
     4981 gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa tttatacatg
     5041 aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta cttatttttt
     5101 atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta atataacttt
     5161 tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata tatgcaacta
     5221 caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac aaggatcaag
     5281 atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa agaacttgga
     5341 gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa catcatcaaa
     5401 acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat cgatatcagt
     5461 tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc tattggtcac
     5521 tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga ttttatcggg
     5581 aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg cattctctgg
     5641 tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga ggatacattg
     5701 gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa tgacaattgg
     5761 ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac gagccctgga
     5821 gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa tcttaatcaa
     5881 tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa tggagaaccc
     5941 ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca tttgcgaatc
     6001 gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa aggattcggt
     6061 aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc ttttctattt
     6121 tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt attgttattt
     6181 ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct tgttccacca
     6241 tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat aatcatcaat
     6301 ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc catcttcaag
     6361 atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt gaagcaattg
     6421 tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac catcaccaaa
     6481 tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata cggctcctgc
     6541 tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga tatttccttt
     6601 gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg catcaacagc
     6661 tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag cagcttttgc
     6721 atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt gatagttttc
     6781 atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc ctgctaaagt
     6841 agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat attttttcaa
     6901 atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa ttgttggttt
     6961 gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc ctatgcttct
     7021 gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt atggttccag
     7081 cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt atactcttcc
     7141 catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc aggtaattcg
     7201 taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt aaagtctcga
     7261 acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc ttcttctcga
     7321 gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa atttcgagtg
     7381 atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct tgataacttg
     7441 atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa tactgcacca
     7501 agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac atcaatttta
     7561 gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga agcatagatt
     7621 tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga tatataggcc
     7681 atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa ttctttcgcc
     7741 acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac taagaacacg
     7801 cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc ataagattgg
     7861 actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc agcacgtaca
     7921 ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac tgctgcatcc
     7981 agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat gacagatttg
     8041 atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa cagtgctgtt
     8101 tctactaatt ggcagtgcgc taaaggccct tccacttgga ttaatggttc atttgcaaaa
     8161 actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt ttgtaaatac
     8221 tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc ggaatcattg
     8281 aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac cgcgtatccg
     8341 tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc agctcgacct
     8401 aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag cgtcaaactg
     8461 tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt catttcctac
     8521 ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg aaaaagcaca
     8581 tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat cctttcttat
     8641 tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg agacacgaag
     8701 aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct actacaatcg
     8761 tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca acataatcaa
     8821 tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat ccccaaagaa
     8881 acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga caagatatac
     8941 cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat acaaagtaag
     9001 aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt ctcgccttgt
     9061 tttttattgt atacattaga tatggagcag gttaccataa agatgattat tagtcgttat
     9121 taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt tggacgtttg
     9181 aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt gacatcttct
     9241 cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt gatttttttc
     9301 ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag cagttgtgtt
     9361 cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa gaattccact
     9421 actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc gattagcttc
     9481 atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat gttaccatct
     9541 ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga tttacctttt
     9601 atcacttaat agtataacat aatttcgtga gaaaaaaata caattttttc ttgaccatct
     9661 cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag tacacaataa
     9721 agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat gatgagattg
     9781 gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc aaaaaagaag
     9841 tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg gatgatatcc
     9901 aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag catcagcaaa
     9961 tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa cagacacttg
    10021 atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg tttaaacaac
    10081 aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa tacggaaaaa
    10141 aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg tatgaagaaa
    10201 tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa gaaccagaag
    10261 atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttctct tacagctggc
    10321 ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc gatgaacggt
    10381 tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta aatgaaatta
    10441 tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta aaatgaccta
    10501 ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg tctgctccat
    10561 tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa acgtataagg
    10621 tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt ctccgcaagc
    10681 ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca aattatcgcg
    10741 tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg ctgcaaccat
    10801 atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc ctgcttgttt
    10861 tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag atggaaaaag
    10921 ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac ttcgccccaa
    10981 cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt ctgtaattga
    11041 ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt cacaaaaaac
    11101 tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac gagacgtaat
    11161 gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct ctttttcttt
    11221 atgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg cttcaatgat
    11281 cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca ctccaatgct
    11341 tttcgttttt ccataactta actgtctggc atttgcatta gggatataat cgacttcttt
    11401 catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc cattgattac
    11461 acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa tgtttgccac
    11521 tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac agctggaccg
    11581 cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg cccgccccaa
    11641 tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac tatattccta
    11701 ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac aaacgcataa
    11761 aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc aagccctcct
    11821 tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt gttttgccaa
    11881 agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat tgaatacatt
    11941 ataatgatac tgtaaatata tctttattat tagtgaatg
//
LOCUS       DAGCWU010000079        11670 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.079, whole genome shotgun sequence.
ACCESSION   DAGCWU010000079 DAGCWU010000000
VERSION     DAGCWU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.079"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            157..677
                     /locus_tag="MBZ71_001663"
                     /pseudo
     CDS             157..677
                     /locus_tag="MBZ71_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(579..1295)
                     /locus_tag="MBZ71_001664"
     CDS             complement(579..1295)
                     /locus_tag="MBZ71_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBT4429622.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1345..1554)
                     /locus_tag="MBZ71_001665"
     CDS             complement(1345..1554)
                     /locus_tag="MBZ71_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429623.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1831..3104
                     /locus_tag="MBZ71_001666"
                     /pseudo
     CDS             1831..3104
                     /locus_tag="MBZ71_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3211..4242)
                     /locus_tag="MBZ71_001667"
     CDS             complement(3211..4242)
                     /locus_tag="MBZ71_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429624.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4249..5079)
                     /locus_tag="MBZ71_001668"
     CDS             complement(4249..5079)
                     /locus_tag="MBZ71_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4429625.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(5076..5891)
                     /locus_tag="MBZ71_001669"
     CDS             complement(5076..5891)
                     /locus_tag="MBZ71_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4429626.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5888..6712)
                     /locus_tag="MBZ71_001670"
     CDS             complement(5888..6712)
                     /locus_tag="MBZ71_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBT4429627.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6702..7802)
                     /locus_tag="MBZ71_001671"
     CDS             complement(6702..7802)
                     /locus_tag="MBZ71_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4429628.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7980..8765
                     /locus_tag="MBZ71_001672"
     CDS             7980..8765
                     /locus_tag="MBZ71_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBT4429629.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8798..9181
                     /locus_tag="MBZ71_001673"
     CDS             8798..9181
                     /locus_tag="MBZ71_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBT4429630.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9257..9388
                     /locus_tag="MBZ71_001674"
     CDS             9257..9388
                     /locus_tag="MBZ71_001674"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4429631.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9357..9593
                     /locus_tag="MBZ71_001675"
     CDS             9357..9593
                     /locus_tag="MBZ71_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4429632.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9671..10642)
                     /locus_tag="MBZ71_001676"
     CDS             complement(9671..10642)
                     /locus_tag="MBZ71_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBT4429633.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            11276..>11670
                     /locus_tag="MBZ71_001677"
     CDS             11276..>11670
                     /locus_tag="MBZ71_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4429634.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRQFRETTVFEDIFA"
BASE COUNT         3481 a         2670 c         2226 g         3293 t
ORIGIN      
        1 aatcctttag aaaaaaggct tctataatat tttgtgttgt tggaaaatcg aaggcgattt
       61 tctactaaca ctttttcttc gtttcaactc aaaaagagac tcacttttgg taaaatttag
      121 tcgaccaaaa catcacccta ccaaaggagt cgctctatgg ataatcatac tagaaaatta
      181 ctcggattaa cagataaaca tttatttttt gatgaagaat ggctgattga aaaagaatat
      241 aaaggcgttc aagcgcagtt tattaaaggg agattggatg actcaccttc ccattgtgaa
      301 cactgtggat cgattcgtat tatccgcaac ggttcctata cgacacgtac tcagatgttg
      361 aaagtcaaag aaaagttgac tattttagaa ttaaaacgta cccgttttct atgctatgat
      421 tgtgggcaga ccttttctgc gaaaacggac ttagttgatg aacatcatca actaacaaag
      481 gaactgaagc aggcgattct catagaactg tacgaaaatc aatcacgtaa tctgaaaatc
      541 agaagaagta ccgtcggctc aagcgatatt ggaaactctt actcaaagat tccacgacat
      601 tggagccgct caaacggcat tatcatcgac tgtttaaacg accaattagt caaacagaaa
      661 tcgtcgatga accgttgtgc acaatggtgt cctgaacgta cagcgacacc ttccatatcc
      721 agagcagtcg ccacatcacg gggatggatg ccatccaaat taaaagcaac cacgccagta
      781 tgatgttcag gatcttgcgg tccgtaaatg gtgataccgc cgatcgcttg taatttaggt
      841 aaaacgtagg caaccagttc ttgctcataa cgatgaatat tctccatacc aatctcattc
      901 aagtaatcga ttgctcctgc aatgttcgga gtaccagctt caaatttcca tggcagctct
      961 ttccatgaac tatcgtaaag attgacaaaa tctatcattt caccgccgaa ttcgactggc
     1021 tccatttgtt ctaacaattg acgtttgcca tataatacac cgattcctgt cggcccgcac
     1081 attttatgcc cgctaaaggc gaaaaaatct gcatccaacg cttgtacatc aactgtcata
     1141 tgcggtgctg cttgggcacc gactggacta tcctcaacta caacagcctg ttcaaccctc
     1201 agccgtttca agcacaacaa ataaccttct ggatctggtt tgccgacctg aagatcatcg
     1261 atcgtcacaa aggaatcaat tttttcctta atcatcggat gtgcttcaaa aaaatagtct
     1321 agctcccgca gttcagaatt gagactactc cgattttcca gtgctgctga tccgctgctc
     1381 aaaatgttca gcatccactg gtctcccaac cgcttgactt atcccttcat aaaaggacag
     1441 ttctgctgcc gtcccaacac cttgttgata gtctgcaaaa ggaattttat agccgaactg
     1501 ctccagtaaa taggcttcag taaatagtga tgaatactct cgtaaaacat ccattcactg
     1561 tcaatcatcg tcccatcaaa atcaaaaata agtgctttgg tcatcttctt tcccccttac
     1621 ccaaaacaag caaaagctgt gtcctgctct ctctacagta tcagcataaa aactgccgtc
     1681 tgaaaagtca aaggtgaaag aaatgtggcg ctataatttc taggactgat agtaaatcat
     1741 tctatcagtc cttttgagca taaaaaaaca aaacatacag aagctccgtt agaattgaat
     1801 caccacaaca caatcaaagg agcgttctgt atgcctgatc ttcatcttat caaactatta
     1861 ctaggaatca aagatggaaa cattcaaatc actcatgtcg agaagaaaaa aatacattca
     1921 atcaacagta tcgttgtttc tgccacgctc attaaaacga ttcagccctg tcctcagtgt
     1981 aaacaggtga atcaacatgg gctgatcgtt aagaacggaa agaaaaaatc gctggtcctt
     2041 ctcaataagt gtgcgaatca acggacttat cttgctttgg caaagcaacg gtacctctgt
     2101 cgtggctgtc acacctattt tacggcgaat acctacattg ttgaaaaaaa ctgttttatc
     2161 gccaaacaag tacattataa aatattggag gaattgactg aaaaacaagc aatgacgacg
     2221 atttctaaac attgcggcgt ttcttggtca accgtttcgc gaacactcgc ttctctgctc
     2281 cccatgacaa acgtgaaaat aaattggttg cctcgctgtt cattggtaga tgaatttcgt
     2341 tcattgaaaa atcaagtcgg tccctattct ttttcttgta tggatggcga tactgggaaa
     2401 ttactggata ttcttccttc acgtaagaag aaagatctca tttcttattt tatgcaattt
     2461 gaacgacgtg cacgtttaaa cgtaaaaata ctcgtaacag atatgaacgc ctcttatgct
     2521 ttgctgataa aagagtgctt tcctcatgcc aaactcgttg tggatcgctt tcacatcgtc
     2581 aagcacctca ttcgcagttt taaggatatt tgactatgag tcatgaaaag cttcgaccga
     2641 aacgatccaa tacaagccaa acactatcga caagtgaagg ctttatctcg acttctgatc
     2701 aaacgacagg acacgcttgt ttatgataaa tggacaaaat ggcgtaattt tggttgggcc
     2761 tatttaaccg aaagtgaagt cgtagaacgt ctactttcta cttctgatga actacgaata
     2821 gcttatgctt actatcaaga aattcttcaa gctttttatg acaaagaagc ggacatcttc
     2881 tttcagcttg taaaaacaat gcccaagtcg gttccaagag aactgcatca tataaaaaaa
     2941 gcgtttataa actatgaatc cggcattcgt ttagccttag agttgcctta ctcaaatggc
     3001 aaaatagaaa atctgcatac ccacatcaaa gcattgaaac gaatagctta cgggttagga
     3061 tgagaaccag aattttctta atcaatcatc tgatcactta ttaaacataa aaaaacatct
     3121 aaagcagaaa atcaattcta ccttagatgt tcttatatca ctctatcagt cctatttgac
     3181 aaagagccag aaatgtttgc cgactataca ctagctttgt tgtgcaaaat gctgagtatc
     3241 aatttccaca gggattgcta gaatatcttt cagctgaatg tataaggcat cgctaatttt
     3301 ctcttcccga tcgttcaacc gatccactaa aatttctttg ccctccagcg tttgaaaaac
     3361 ataccgttga atgttgccta aaataaatga ttccttccat agaaccggaa tcttaacccc
     3421 ctgaggaacc ggttcaaatt gaatcagctc cggtcttaaa taataaatgc agcgattgcc
     3481 cttagcggaa aactcgtggg tatactgatt cagctcatac cattcgaaac gattatagtt
     3541 gccgataaat tccgcaacaa atcggctctg aggattctga tacaattcct tcgaggtgga
     3601 aacctgagcc acccgcccag cttccatcac aaaaatccga tcactgattc gcatcgcctc
     3661 ttcttggtca tgagtcacaa aaatcatcgt catccctagc gattgctgga tagcgcgcaa
     3721 atcccgctgc agctgttttc taattcgggc atccaaagca cttaatggct catccagcag
     3781 caggactttc ggttcagtga ccaatgagcg agccagtgcc acccgttgtc gttgaccgcc
     3841 agaaagaaac tggacatttt tatctgcctg ctccgtcata tccaccaatg atagcatctc
     3901 aaaaaccttt tcctgaatca cctcaggact cagcttcttt accttcaaac caaaggctac
     3961 attttcaaaa acagtcatag ttggaaacaa tgcatactgt tgaaaaacaa agccgatatt
     4021 gcgatccttc gtaggcttat cggcaatatt ctcattgtcc agcacaatct caccgctctt
     4081 tggttgttcc aatcccgtga tacagcgcag caacgtagac tttccgcacc cgctgggacc
     4141 tagtaaagta accagctcac ccttttcaat ctgaatttca tcaatagcta aaatctgttt
     4201 ttcatcaaaa aacagattga gatcatgtat ttctaaaaac attttccgtc acaactcctt
     4261 taagggcttc tttttcgact gcttctcttc cctcgtctgc ttaagggaca gctgttgctt
     4321 ttgtcgataa gtcagcataa aggtagttaa agcaattccc accataaaca gcaaatacaa
     4381 aaccatcaca gcgctagcca aatggccgtt ttcgttcatc cgccgcagca tatagatgcg
     4441 taccgtctga aatcgaccgc caatcaacag gttcgtcagc atgtactccc caaaaaccga
     4501 agaaaaaatc atcagagaaa ctagggtcac accgatacgg atgtttggaa acagcacctg
     4561 gataataatc gtagacatcg gggcgcccaa ggtttctgct gcatccacta attccttcag
     4621 attaattaag cgcatctggt tgttaagact aagatagaca atcggcaacg ccgtaataaa
     4681 caatgcacct accaacacta caaacattgg gataccagtt tcagcatacg ttcgaagcaa
     4741 agccgttacc aaaatcactc ctggtaaagc atacggcagc aggagcagct tttccagcca
     4801 acgattcaaa cgagggaaat acaaatgaat ccaaataatg gtgggaatca tcaacaacag
     4861 aatcgtcacc aagaccacac ctgctaataa aagtgagcga cccaccgcag caataaattc
     4921 cctgtccgtc agcagctgtt ggaaccattg caaggtaaaa tcactaggta aaagcgattt
     4981 tgaccattta gtggagctgg catacaaaat cgttccaata atcggcagca agtaatacag
     5041 gacaaagcag aacaaaatcg gttttattaa ctttttcacg tctgcaccac cttgcctttc
     5101 ttttgaataa tcaacattgc cgttaccagc aggacaccta aagcgactgc caacgcactg
     5161 cctaaattag gcttggcgaa cacatctccc gacactaaag ccgcaattcg aatcgttaaa
     5221 agatttacat tactgctggt cagggcatac gctgtttcat aggtccccat gccattagca
     5281 aataagatga caaaggtgct ggcaatcgat ggtcgcaaaa aaggcaaagc caccttgaac
     5341 caagcatacg ctgatgaagc gcccagcaat tgtgcggact ccagccattc ctttttcact
     5401 tccttcatgc tgggatacaa aaacaagacc cctaacggaa tttggaaata ggtatacgtg
     5461 atggtcaatc cccaccaaga atacaaattg aagtccgcta gaaatggcaa aatcattttc
     5521 agcacaccta cattccccaa caaaataata aaagagaatg ccagcggaat cccagcaaaa
     5581 ttagctgcca aattggaaac aaaggtgatt ttctcctgca atttagtcga tagattcatt
     5641 aagcataaac ataaaataaa cgtcccgatt aacccaatca cactggagct gacagcaatc
     5701 agtacgctgt taacaaaggc ctgtgtgtaa tatttgttgc tgaaaatatc actgtaattg
     5761 gccaatgtcc actggttagt taaatcactg tgcaagctgc acagaaccat aaaaagcagc
     5821 ggcagcagta aaaatgcaca gaagataaag gaggcaggaa ccagaaccaa gcgttttttc
     5881 atctctttca tcccctcatc aattcttgaa tgtcctgcat tttctctgac ggtgcaattc
     5941 ctaataacca gcaaaacaat ggagccgcca gcagctgtgg tacagtttgt tccttcagct
     6001 tttggcgttt atttgaaatc aaataaaaag gaacttcacg atgtgcagcc aagctgccgc
     6061 catgcagtcc gtgcgcatcc atcccgtgat ccgcagtcac cacgacttga taaccttttt
     6121 gcagccattc aggaaggtaa atagccaata atctgtccgc ctgattaacc gctgctgcat
     6181 attcatgaga atcggcggta aatttgtgac cgacatcatc aatattcatc gaatgtacga
     6241 ccaaaaagtc tggctgatac tgattcacta aataatgggc atccgcaaat aaatgcgaat
     6301 cagggtaagt gtcctcacta taaaagatgc catgctgaat cagtcgctcc tcctgatgct
     6361 ggatgcgatc aaccaaacga ttataggggg cttgattgta caactcgctg taccagtggt
     6421 aagcggcggc tcctgttttt ccgccagctt ccttaactaa ttcaaacagc gaccactctt
     6481 tggatcgctg agcatggtaa ttactataga tgccattttc ataggctgga acgccggtca
     6541 tcagtacttc gtataacgga cgtgaattgc tgggcagctc actttttacc ttcaataagc
     6601 ttgcttgctg gtgctcaact aaatgattca tgaacccaag ctgggcgacg gctgtgtcgt
     6661 agcgacagcc gtctagtata actacaatca gtttgtcaag tctactttgc ataggtcagt
     6721 acctccgcct gccaaagctc tggaagctct acagaggtct tgttccaagc ttcattgtct
     6781 tcaactggca ccgccgcttc ataagcatcc tccggcagta atttttcagc tacttcttta
     6841 ggcagctcta ccttgcgaat aggtcgcgca tagccctttg ccaaattgat ttgcccttca
     6901 tcagataaaa tatattcccg tgctagcttt gccgcattgg gatgaggggc atttttattg
     6961 attaaggttg tatacccgct aatgatggag ccgtctgttg gcacagttac ctcaaaacgc
     7021 tcggagtcaa tttggtcacg ataatttagt ccattgaagt cccaaacaac tgccacatcg
     7081 atttcacctt tttcaagatt tgctacgctg gcatcaacgg tggacaaacg gcctttttct
     7141 gccatttttt tgtagtaatt cagacctggc tgaatatcct tttcgtcacc tccgttggca
     7201 atcgccgcag ccaatacagc aaattgtgct tggttggcaa tcgtcacatc ccccacgcta
     7261 acttgatagt caccctcttg cagctccttc cagctagtcg gtgcttgctt cacattctct
     7321 ttatcggtga taaaggaaat ggttcccgta tagcttaaca accaatcccc atcatcatcc
     7381 tttgcccaat ccggaatttc ctcccaatag ttggtcttat aaggtaaggt taactctttg
     7441 ctttcagcca acggaccgaa gctgataccg acatcaccaa tatcggcggt tccatttttt
     7501 ccttctgatt caaacttagc caattcttct gcactggaca tatccgtatc cacgtgactt
     7561 aagccgtatt ctttttcaag gtcgttccaa gtgcctaccc aatttgccca agtatccggc
     7621 attcccaagc tggcaatctc tccttcttcc ttggcttttt ctataatcgt atctaaagag
     7681 tcgtctgccg cagccgattg cttcgttgct tcacctgaga cgcagcctgt cagcaggata
     7741 cttactgttg ctgcacaaaa taaaccgata gtcatgttac gaattttttc attcattttc
     7801 attcattctt ctcccctttc gataattcca ttctaacaaa gctttgtaat gagagaatca
     7861 gtttcttgaa aaggtttcat gaacaatgta aaggttgtat atggtttgta aatttcgacg
     7921 aattcccgtt aaccttctaa agcttttgcg gtatgatagt agaaaaaagg aggctcccta
     7981 tgcagtatta tgatcagcat ctccacacgt atttttcctt tgattcagag gaaaaatttg
     8041 aaaactattt agcctatcag ccggagtttt tcgtttctac ggatcacttt gatttaaaca
     8101 atccatctac cggaaaagat gacattccgg attatccagc ctatataaac aaactggact
     8161 ctttaaaaga aacctattcc accaaatttc tgcgaggtat cgagattggc gttgttccag
     8221 gacaagaaga gcggatcgtg gattacctcg ctcagcatcc ttatgattta aagctggtaa
     8281 gtattcatca aaatggccgc tttgattata tggacgatgt ggttttaacc aaagatccca
     8341 ttcaaactac caaggaatat tttgatcaga tgttgcaggt cttgacggat tttccagaag
     8401 gagatatttt gactcacttt gattatggat tacgccgctt caccttcaca gcggaagaat
     8461 tcgcggagca ttttgaggct cagctgctaa cgattttcaa aaaggttgtt gaacgagaat
     8521 tggcgatgga gttaaatgcc aagagcttct tgaaatacgg caacgaagac ctttaccgtt
     8581 atgccgttcc tttgtatcaa agtgtcggcg gaaagctctt taccttaggc tcggatgctc
     8641 atgttgccga agattatcag ctgggcttta aaaaaatgag tcagctgttg gcagactgtc
     8701 aggtagaaaa gttgctagtc attcaaggaa aagaacgccg cctgacgccg ctgccgattt
     8761 tgtgattcct atataataga agaaagtggt gaaaaaaatg agcaacctgt gggactacaa
     8821 tcaagaggca cccatccatt atttaattgc gcgacattgg gatgcgttga aaattgaggc
     8881 ggtttgccga tccttgttag cagccgttcc aaaacaacaa ctggaaaatt ttttggtggc
     8941 tgacagcttg cagcgggaaa aagttcaggc ttattttgca gcctttaaag atcagccttt
     9001 ggaatatttg catgcgcagt ttcatctttt ttatcaagta gccgccccag atgactacaa
     9061 cgatttacga ggacagctgc aattgacctt tcaagcggat gaaaccgcct ataccgtttt
     9121 attgggaatg gctcgccttg gtgatcaggc gaaggtcgag tggcggattt tcgatatttg
     9181 atggcaggat caaggcgttt cagttgcaaa ccttcctctc tttcaattag aaaaaccgca
     9241 gcaggaagaa gtttgtgtgc tggtcaccga tgctggtgaa attcgtctcc gcttatttcc
     9301 tgaccaagct cccctggctg tagccaactg gcgctcccta gctgagcaag gcgtttatga
     9361 caacacacct ttcggctcgt gtcattaagg aatttgttat tcaaggtggc gctttggatg
     9421 gttctggtga tgaagctgtt tccagctatg agggcttttt cgctgatgaa gtgcatcgcg
     9481 gactttatca ttttaatggc gccttggcct taggggacca cggcccccac accaacggca
     9541 accagttttt tattgtgcaa aacacaaagg cgcaggctga cctgctaatg taaagcaagc
     9601 atacttggag cttggaggac taccggagct ggatggtcgt tatactgttt ttggacaggt
     9661 ataccaaggg ttagaaatcg tagcagcgat cgcagctcag ccgacaaaca gtgaagatgt
     9721 accgattgag ccagtgttca ttcgacaaat taacttcgaa aaaacatcgt aaaaagacac
     9781 tcactgactt cagcaaaagc tctattcttg agctggctgc agctagtgag tgtctttcat
     9841 ttatccataa gataaagctt ggattcaata aataaataaa tgttttggtt aaagctgtga
     9901 ccagcaaatg aggaaccgtg cgagccagtt gatctgccaa tgcaaacatt tctttaggtg
     9961 aaggcgctgg cgtgtctgcc aacggatact ccagtcccaa tgcttggtaa ccgtgcatac
    10021 catagcagtg gtacggtagt aattctaacg gtacgaaccg atcaagatct gccacaaaac
    10081 aagcaatcgt cagcagctcg gtcaggttca tattgatacc tggaaccaca ggaactcgaa
    10141 ttcgcacttc gcagttagga tactcaatta aaaagcgacg aatattcttc aaaatttgtt
    10201 ggttatctaa gccggtccat tgtttgtgga gcttgctggt aaacattttt acatccacaa
    10261 acaatgtatc cacataggga gctacctttt ggatttcatt ataagcacca caaaagctgg
    10321 tttccagtac ggtattgatt ccaatgtatt tcgatttttg caaaatttct ttagcaaaat
    10381 ccgcctgaac cagtgcctcg cctccgctaa tggtcactcc gccatccgag tgaaagtaaa
    10441 aaacagcatc cttctcaatc tcatccatta cctcttcagc ggtcatggtc tggccaaatc
    10501 ctttttcaat ctgcttgctt tgtgattcgg gaatcgaaca ccaggcacag cgtaatgggc
    10561 agccctttaa ataaacaacg gtccgtaaac cagcaccatc atgtagagaa ccctgttcta
    10621 ttcttaatac tgttccttcc atatagattt ttgaattaca cactagttta aactaaaaca
    10681 cacaaggaaa aacttaccat ttaatcataa aattagctca tataaatata ctaggatgta
    10741 aaatagactg gtcaaaatct ggtcaaaaaa gaggactaat acaggtagtc ctcttttttg
    10801 ttattttaat attctaaatc attttttatt ggtttattcc cttattcgat gtagactcaa
    10861 atcactatat cactagatat caaacggcgt catacggaat caaaaacatc aaatttgacc
    10921 tgttaaatgg tcattgaata acattcaaaa tcaataaaac ggagtcagac acggagtcaa
    10981 aaacgctgag agtaaaaaat atatacagcc ttgtaggtaa agtaccacag ggctgtatat
    11041 tttttatttc agattacctt aaatgattcc atttactttt tatctcattt tttcctacta
    11101 gttagtatca aatactctcc tattttctca ataaactatt tttggataca tggtatctga
    11161 aacagctaaa accttgtcat tacagcaaaa aatcacgtta tccagactgt agacaaagtc
    11221 cattttatgg atgatgtcta cagtcttttc ttttataata gatttatgaa aacgaatgca
    11281 aggagtgata gctatgatgt ctaaaaacaa tacgaatggt agaaatcaat ttgcgatgct
    11341 tacgattgat gatctagttt ctcaagatca tctcgttcga aagattgatg ccgcgcttga
    11401 ttttgaattt atttatccaa tcgtcgaagc cacttactcc gatttaggtc gcccaagtat
    11461 cgatccagtg attctgatca aattggtgtt tattcagtat ttattcggta tccgttccat
    11521 gcgacaaacc attaaggaag tcgataccaa tgtcgcttac cgctggtttt taggctactc
    11581 cttcgaggag aaaatccccc atttctcaac ttttggaaag aattatgttc gccaatttag
    11641 agaaacgacg gtgtttgagg atatttttgc
//
LOCUS       DAGCWU010000080        11657 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.080, whole genome shotgun sequence.
ACCESSION   DAGCWU010000080 DAGCWU010000000
VERSION     DAGCWU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.080"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            164..3391
                     /gene="fss3"
                     /locus_tag="MBZ71_001678"
     CDS             164..3391
                     /gene="fss3"
                     /locus_tag="MBZ71_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBT4429635.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3503..3817
                     /locus_tag="MBZ71_001679"
     CDS             3503..3817
                     /locus_tag="MBZ71_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4429636.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3830..4204
                     /locus_tag="MBZ71_001680"
     CDS             3830..4204
                     /locus_tag="MBZ71_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4429637.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4205..4558
                     /locus_tag="MBZ71_001681"
     CDS             4205..4558
                     /locus_tag="MBZ71_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBT4429638.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTGEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            4627..5643
                     /locus_tag="MBZ71_001682"
     CDS             4627..5643
                     /locus_tag="MBZ71_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBT4429639.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6237..8111
                     /gene="ltrA"
                     /locus_tag="MBZ71_001683"
     CDS             6237..8111
                     /gene="ltrA"
                     /locus_tag="MBZ71_001683"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBT4429640.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8237..8593
                     /locus_tag="MBZ71_001684"
     CDS             8237..8593
                     /locus_tag="MBZ71_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBT4429641.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            8638..9780
                     /gene="dcm"
                     /locus_tag="MBZ71_001685"
     CDS             8638..9780
                     /gene="dcm"
                     /locus_tag="MBZ71_001685"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBT4429642.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9770..10059
                     /locus_tag="MBZ71_001686"
                     /pseudo
     CDS             9770..10059
                     /locus_tag="MBZ71_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10315..11499
                     /locus_tag="MBZ71_001687"
     CDS             10315..11499
                     /locus_tag="MBZ71_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4429643.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11496..11609
                     /locus_tag="MBZ71_001688"
     CDS             11496..11609
                     /locus_tag="MBZ71_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBT4429644.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         3909 a         1994 c         2584 g         3170 t
ORIGIN      
        1 aatttaaagg cagtatattt cgtattttat attaaatttt taggctcatt cattctcaaa
       61 tccattctat ttagtcaata aatattttaa aaagacttta taaacaaaga tgttcaagcg
      121 agcatcttat ttttttgtgc ctaatttgaa aggagaacat ttcatgtata aaaaacaaaa
      181 aaagaaatta tgtgtattaa ctattttgag tttggctgtt caatcaattg taggagcaat
      241 tgctcctacg attgcttttg ctgatgaaat tacccatcca caaacggtaa ccgtagaact
      301 agatttagca catcaatatg ctgttgaagg aacttttagt gatggtcgtc caatgtcaga
      361 agtcactgtt ccgcattatg cagtgtacaa cggcgttaaa caagatattt tctgtgttga
      421 accaggagtc ccgatttaca atgagttcac tcctggatat gagaagaacc cgttaccaga
      481 tatgtccgat aaagccaaat tagtttccgt tttatggaaa aaagtaggta cagatgcaga
      541 tactcatatt gtggcacaga agatgatctg gcaagaagtt aatgggtata cccttcactc
      601 aataaaacga tcagatggta gtgccgtaaa tatcgcagcc attgaggcaa aaatcaatca
      661 agccattgcc gattatcaga agaaaccaag cttccataat agtacggcta aaacagtatt
      721 agggcaatcg actactgtga cggatacgaa taatctgaat ttgtcagagt tcgatgaggt
      781 tgtggaaaat accgcaaaca ttgattatcg tgtgaatggc aatcagttga taatcagccc
      841 aaatgccaat tcaaaagaaa gtggtgtgtt gacgcttaag aaatctgctg gtaccggaac
      901 acctgtcgcc tataaaaagg ctggccaaca gactttaatg gccggggcga tcgataagcc
      961 gaacacttat acggtcaaaa ttgatgtgga aatggagggt tctttgaaga ttaaaaaagt
     1021 tgataaagaa tcgggtgata ttataccagg aacggttttc catttagact ttggaaaaac
     1081 tttacccgca aaagacgtga ccaccgataa agaaggcatt gcgaccttag atgagattcc
     1141 tcatggtaca aaggtaacca ttactgaaaa atcggtgccg gcgccttata cgattgatac
     1201 cacacccatg actgccacaa ttaaagcagg tgaaaccatt tcagtgactt caaaaaatac
     1261 gcgagaaaaa ggtcaaatta ttctggataa aaccggaatt gaaacaggga gtgatctttg
     1321 gaacgataat tactctttag cgggcaatac gtttgccatt cgcaaggata gcccaactgg
     1381 tgaaatcgtc caagaaatga ccacggatga aaaaggtcat gcagaaacac caaaagagat
     1441 tgccaatgcg ttggaattgg gaacctacta tgtgactgaa accaaggcta gtcatggctt
     1501 tgtgaatact ttcaaaccag taaaagtcga attgaattat gtcaatcaaa cagtggctct
     1561 tgttaccagt aacgtaaaag ggcaaaacca agaaattact ggtgaaacca ctttgacaaa
     1621 agaagacaaa gataccggtc atgaaactca agggaaagct gagtttaaaa gagctgaata
     1681 caccttgttt actgttaagg atggaaaagc ggtcaaatgg accgagaact ttaaaccaga
     1741 attgttgaag ggaacgaaag cttctgatga aaccgtaact ttgactttag atgaaaagaa
     1801 tcaagttgcc gttaaacact tagcgattaa tgagtattac tggcaagaaa ccaaagcgcc
     1861 tgaaggatat accttggatg aaacaaagta tcctgtatcc atcaaaaagg tggatgatag
     1921 cgaaaaaaat gctgtgatta ctcgagatgt tgcggcaaaa gaacaagtga ttcgctttgg
     1981 ctttgatttc tttaaatttg ctggatcaac tactggcact gccgaaactg gcttcaatga
     2041 tctaaccttt aaagtgtcgc cattggaagg aaccaatgaa atcaccggtg ccgaagatga
     2101 agcgaccaca gcttataacg agcaattagg gtttgatggt tatggcaagt ttgaaaatct
     2161 tccttatggg gattatttac ttgaagaagt agaagcccca gaaggttttc aaaagattac
     2221 gccattagaa atccgttcta cttttaaaga aaataaagag gattatacca agagtgatta
     2281 tatctttact attaccgaaa aagggcaaaa gcaaccgatt aaaacggtaa cggtccctta
     2341 tgggaatcta accaatgaga atttctcagt cagtttgaac cgtttgatgc tttatgattt
     2401 gcctgaggaa gaagatagct taacttctct tgcgacttgg aaagaaggag ataaagaatt
     2461 gactactctt gattctaccg agctagtcga taaattgagc tataatctac atgaaatcaa
     2521 agaagactgg tatatcgtag ctcaagccat cgatgtagat gctacaaaag ctgcccaaga
     2581 aaaagataaa aaagccaaac cggtggcgat tgcggaaaca accgcaactt tagcgaataa
     2641 agagaaaaca ggaacttggg agattctgca taaattaact gtcgaacaag ttttagacaa
     2701 gaccattgtc ttattcaact atgtgtatga aagcaaagaa gcctatgaag ctggtgatga
     2761 gccggtagcg aaggatgcta gcttgaacaa tcaagcgcaa actgttaatt gtatggtgga
     2821 acgtcatgtt tccatccaaa ccaaagcgca cttagaagaa ggttctcaga cctttactca
     2881 tggtgacgtg gtagatatgt ttgatgatgt atccattacc catgatgtac tagatggctc
     2941 aaaagaagct tttgaaacaa ttctgtacgc tttatttcca gatggtacaa gtaaagaaat
     3001 ttggaaatct ggcaagattg attacgaagt gaatgacaaa gaatttacca aaaccgtact
     3061 tgcggaaaaa gtggatacaa gtaaatatcc agaaggtaca agttttacct tcaaagaaat
     3121 taactatgac aaagatggga atatcaatgg aaaacacaat gaagatttga aagaaaaatc
     3181 tcaaacttta acgcccaaaa aagttccgac aacaccaagt acaccagaac aatctgaaat
     3241 gccaactgtt ccaagtggct ctcaagaatc tagtcctaca gtgaaggcat tccctcaaac
     3301 cggggagaaa acttccaatg tgctactgtt cattggcttt agcttgatct ttgcgacggc
     3361 tggctattat ttttggaatc gtcggaacta aggtgataag atgaaacatg taaatagtag
     3421 aaaacaatac gcgcgaccgc ctcctaaaaa agtggtcgcg ctatttttgt attcaaaact
     3481 aacaatgaaa agaggaaaaa atatggaatt gaaatttgtt gtaccagata tggtcgagac
     3541 gttcggaaaa ctaacatacg gtggtgaagg tgaagttcta tcggaagggt atggtcgaaa
     3601 tactacaatt gttggtcgaa gttaccatct gttttcaaat aaacaacggg ccgatgatat
     3661 tgaagtggtg gtagctgcgg aagccggcga aaaggatttt gaccaagatc aaccactaaa
     3721 agctgtaaat ccccacttaa ttgccaaagg ctatgaaatt gagaatcgtg ggtttaccga
     3781 ttacgtgttg tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac
     3841 agaaggtatt gtcgtagatt caggattaac ctttggcaag ttgcgttttt ccgcattacg
     3901 tcgagaagta cgcaagcaaa atgaggacgg aacggttagt aacgaggtaa aagaacggac
     3961 ctataatttg aaatcctccg cgcaaggtcg aatgatccaa gtcagtattc cagcaaatgt
     4021 acccttacgc gagtttgctt atgatgcaga ggtagagctg gtcaatccaa tcgtggatac
     4081 ggtcgccaat tatgtttttc gggaaggaac caccgttaat tggtttatca aggcagatga
     4141 tttagtcttg aaacgacaac tgaatcaagg aaaacctacg aaccaaaatg aaggaaagaa
     4201 ataggtgaag aatatggaaa ttaatgttga acatatttta gattgtctcg atcaatttgg
     4261 caaaggtgag ctaacggggg aacaactcac taacgcatta acctatgatg aaaaaatgtt
     4321 tctgattatg catcaagggt tactggaagt aggtaacgat gccaaagaag actttgatgt
     4381 actggattgg ttaggggaag aagagtcttt ctttatggtt gtcgaggtaa atgaaagttt
     4441 atgtcggcag gcagaatctg ttttggaaga aatcgatgtg gagatgccgg atgccatcga
     4501 aagattttta aaacaattag tggaaacgaa acaacttccc gtagtagtaa atgactagca
     4561 taactttttg aaattgccca atgaaatcct attgggcttt ttcgactgga ggtggaaacc
     4621 atcaaaatgt acaaaggaca tcgcatacgc gcaggagatc aacacttgat ttatcacttt
     4681 gttcttggct ggctaatctt tttgttcatt ggttggatga gcgtctttta tttacaagaa
     4741 cttagacaat ttgatatttc taaggtatca ttttccacta gtgaaattgg ctggtctatg
     4801 aaggatgtaa tctgcttgct aggaagtctc gctctttcag gaagcatgat gttactttat
     4861 atacactttt ttcaggatca ttggcgaagt ctttggcatc gacaaaagtt agctcggatg
     4921 attctagaaa accattggta tgaagtaaag cagactcaag gtgaaggttt tttcaaagat
     4981 ttgaatagta gttggaccaa agagagtatc agttacttcc ccaaaattta ttatcgaatg
     5041 aaagatggct tactttctat tcgcgttcaa atttcactgg gaaaatatca ggatcagcta
     5101 ttgaagttgg aaaataagtt agaaagcgga ctgtattgtg agttagtaga aaaagaactc
     5161 aaagactcct atgtggaata caccttgtta tacgatatga ttgccaatcg aattgggata
     5221 gaagaaatag tggcagaaaa tggggcgttg cgattgatga aaaatcaagt atgggcgtat
     5281 gattccttgc cgcatatgtt aattgctggc ggcacaggtg gtgggaagac ctatttctta
     5341 ctcaccatta ttcaagcact attgaagtca gatgcagaat tgtttatcct cgatcccaaa
     5401 aatgcggacc tcgccgattt aggtacgata atgcctcacg tatactccca aaaagaagaa
     5461 atttctgaat gtgtggaaga tttttacgaa cgcatgatag ctcgtagtag gtcaatgaaa
     5521 gaaatgccca actacaaaac aggagagaat tatgcgtatc tcggacttcc gcccaacttt
     5581 ttaatctttg atgaatacgt ggcttatatg ggtgcgaatc gttttcctac aagcatcgaa
     5641 tagatgtgaa atggaagcct attgagatta tcaatagtga caactaatgt tttgagagat
     5701 ggaatgaaag ccttcaagtt gagctgaaac atccccgcta atactcctac atgctctaat
     5761 caatgaagta gattggtttt ggtatgaagc taggtgaagt cgtctgaaat ctgcctaagt
     5821 gcttgagcat aaggctgact atagggcggg cggctgaaaa agataactat ggtaagaatg
     5881 tctgaaaatg actgacgaat ctgcgaatgt acgggtctaa aaggctgatc ttattgaaag
     5941 ataggaacat acggacgtat ggtgagatac cgacaagtaa ggttccatga tatgaaaatc
     6001 gggatagcta acgaagaagc tataatttca caggctcata gtagatacct aaggttatac
     6061 atgatagcta aaaacattgg aacgatgtaa gcagaaaact atcaattaaa tgactacttc
     6121 tagataggtg catataagta ataacgaaat tgctttgttt ctgtgaaagt aagggcacag
     6181 taccgatgaa gcgtctaaca aatgtggagg gatagcccta agtctattta ataacaatga
     6241 atgttgaaac agatgaagtt caaggatctg gtaagaacgt gggaaaatcc gttatggagg
     6301 tgccacgctt gaacacaaaa atacgttact gggaatatta tgatctacaa gaaacattcg
     6361 atcagctatt tactcaaagt agaaacggga aaaagtttta tcaattatat gagttaatca
     6421 tttctgaaaa taatattctt ttagcatacc gtactatcaa ggcaaacaag gggtcttcga
     6481 caccaggaac ggattccttc acaattgata actataagga aatgaaccaa gcagagttta
     6541 tccatcttat tctcagtcat ttagaaaatt acaaaccaaa atccataaag cgagtaatga
     6601 tacctaaacc caatggtgag aaacgcccat taggaattcc atgcatgatt gatcgaatga
     6661 tccaacagat gttcaagcag atactggagc caatatgtga agctaagttt tatgaacata
     6721 gctacggttt cagaccattg agaagtgcaa agcatgctct aggtcgcatt atgtatctca
     6781 taaacatcag taaaatgcac tatgcagtag acattgatat caaaggtttc tttgataatg
     6841 taaaccaccg tttactgata aaacaattat ggaatatagg aatttgtgac aaacaagtct
     6901 tagctattct ttcaaaatct ctgaaatcac ccattcaagg agaaggtatc ccgagcaaag
     6961 gaacgatcca aggaggtatc atttctccac tattatctaa tgttgttcta aacgacttag
     7021 accattgggt ctcaaagcag tggcatacct ttgaaacaaa gtatccctat actaaaggtt
     7081 acaataaatt tcgtgctcta agagatacaa atctaaagca aggctatatt gtcaggtacg
     7141 cagatgactt taaaatcatg actaatgatt atccgacagc attaaaatgg ttccacgctg
     7201 tcaagcttta cctaaaagac agacttaaat tagatatttc caatgagaag tctaaaatcg
     7261 tcaatttgcg taaatgtaag tcggaatttt taggattcgc tatttgcgtg aaacaaaaag
     7321 ggaaaaaatg ggtttgtaat tcacgtattt ctaacaaaaa aaaggatcag attaaagaag
     7381 agattaaaca aagaatcaaa gatattcaaa agagtcctac agcacaaaat gctctcctgt
     7441 ttaatgctct cgtgctcggt gttcataatt atttcaggta tgccactcat attaatttag
     7501 atttgaatag gatgaactac gttctttcat tcactactta caaccgatta tcaaattgta
     7561 gtacaagatg ttaccccaac aaagcttccc caacttacaa aaagttttac tcaacaacga
     7621 tgaaaacgta taaagtagct ggagtctatt tgtaccctct ttgcgacgta tctacaaaaa
     7681 caatttacgg atttaatacg gaggatacac cgtttactcc gtttggtcgt caaagattgg
     7741 agcacaaaag cctacagtct cttgtgttcc aagaattaag aaaattgatg gaatcgaaaa
     7801 taccgaatag aacggttgag tatttagata gtagaatatc tcgttactca atgaaatcag
     7861 gcaagtgtga gattacaaaa caatttttgc cagcaaaagc cgttcactgt catcactatt
     7921 tgccaaaaag tcttggtgga gacgacaaat tcgataactt gagaattatt cataaggata
     7981 tccatcttct gatccataca acaaataaaa tgataatcga tcactacgtg catgaactga
     8041 aactggtgcc agagcaaata gcaaaaatca acctttatcg aaagatgtgt aatctgcaaa
     8101 acattcaata gaaaataaat agatggaacg ccgtatgagg tgaaagtctc acgtacggtg
     8161 tggagcgggg gaaaagatgg agattatatc aaattcttac ctatcgctat agatgttaac
     8221 gacaaaagaa agtgcggtga ttttgaataa gcttaaacaa atcgtgatgt taggccgtca
     8281 atctggtttc tttctgattc tagcttgtca aaggccggat gcgaaatatc taggagatgg
     8341 gattcgcgat cagtttaact tccgtgtggc tttgggtcgt atgagtgagc ttgggtattc
     8401 catgatgttt ggagaagtcg ataaaaattt ttttatgaaa cgcatcaaag gacgaggtta
     8461 tgtagataca gggggcagtg tgattagtga gttttatacg ccacttgtac caaaagggta
     8521 tgatttctta agggaaatta gcaacattgt tggattaacc gttcatacgg aaagagaaaa
     8581 taatagtgtg tagcctagga atatcaataa tcctgggctt ttttattgag gtgatttatg
     8641 acatttttag atttattcgc cgggattggt ggctttcgct taggtatgga acaagccggt
     8701 caccagtgta tcggtttttg tgaaattgat gaatttgctc ggaggagtta taaagcaatc
     8761 catgatacaa ggaaggaggt ggaaatgcat gacatcacaa gtgtatcaga cgaatttgtt
     8821 cgatccctcg gaccagtgga catcctttgc ggcggctttc cgtgccaagc tttttcaatt
     8881 gcgggaaagc ggcaaggatt ttccgatact cgaggtactt tattctttga aatcgctcgg
     8941 ttcgccactc ttctccaacc taagtttcta ttccttgaga acgtccgggg attgctcaat
     9001 cacgaaggag gggctacgtt cgagacgatc ctccgaacgt tggatggatt ggggtatgat
     9061 gtggaatggc aagtgcttaa ctcaaaggcc tacgttcccc aaagccgtga gcggatcttc
     9121 cttatcggac attctcgaga tgcatgtacc gaacaagtat ttcctatcat tggatcgtct
     9181 tcaacatctg atcaaaacat cagaaacttg gtaaatatca atccttccaa tcgaggaatg
     9241 ggtggccaag tctatggttc agatagcgta gcaccaacct tgaccggtga tgaaggaatt
     9301 aaaattgcct taccggtaaa ggatgggatt tctgtagcgg gtatgttgtc gggaaatttt
     9361 gaacaaggca ataggattta tgaggtaacc ggaacagccc ccactttgtc aacgaaacaa
     9421 gggggaacga agattatgat tcggaaaaat gaaacagaat atcaagaggt gaagccaggg
     9481 gacagtgtga acctggcttt tcctaattcc acaagtcgcc gaggtcgtct gggaaaacaa
     9541 agtgtgcata ccttactaac aggagaccaa caagcggtag tcacggatca gtaccagatt
     9601 cgaaagttaa cacctaggga gtgctggcgg cttcagggct ttccagattg ggcttttgat
     9661 cgagcttctc aagtcaattc agatagtcag ttgtataaac aggccggaaa ctcagtaaca
     9721 gttccggtca tttttgatat tgctagaaga ttaaaggggg taaaagcata tgatttatga
     9781 cgagttgatt ggagaaatct actgggtgat tggcaagatt caggcagatc cgaaattgga
     9841 agaagaactg taccggctca attttgatat tcgcaaaaac ggggtgaaag tccaggtgat
     9901 tcgtatgtag tggatgagga aacggatgct cgaatggagc tcaatcaagt catcggtgaa
     9961 ttggaacgaa ttgcggacct tgccaaagaa cctgatatcc gccaatactt gtttgagata
    10021 aaagcagagt tggaaattga gggtatcacc gcggagtaat agggaaataa cccactgata
    10081 ggcactgacc gcgggggatt tgcgaagcag aaatcctccg tggtgctgtg cctgttcatg
    10141 gacggcggca gccggccatg atgtctcacc ccccgtatct aacagggggg tacaaaaaca
    10201 caaaaaacaa gcaagtaacc ataaaaaatg cttgttgtat caaggatatt aagtaatttg
    10261 tataggtgtt aactcatttg gaaaagttaa caccttgaat ttggaggtga aaacttggcg
    10321 caacgaaatt tagattatcg gttattaaaa gaacgacgca acgaatatgg cgtttcacaa
    10381 aataaattag ctactgcttg cggacttagc cgcccatacc taaatcagat agaaaacggt
    10441 ggcgtaatcg cctctacaaa gaccatgaga aaaatttttg atcaactgga aagtttcaat
    10501 cccgatttac ctttaacgtt actatttgac tatgtaagga ttcgctttcc cacaacggat
    10561 gtgcggaaaa ttattcaaga aattctccat ttgaaattcg agtatatgct tcatgaagat
    10621 tacgcctttt actcctatca agaacaatat gtcatgggcg atattgtagt gatgttatct
    10681 catgaagaag ataaaggtgt ccttttggaa ttaaaaggtc gtggttgccg gcagtttgaa
    10741 acttttttac tcgcccaaaa gcgtagctgg tacgactttt ttgaagattg tctaaaagcc
    10801 ggtggcgtga tgaaacgctt ggatttagca atcaatgatc gagtggggct actggatatt
    10861 cctgatttaa cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg taccttcaaa
    10921 agttaccgtt ccggggaact gttgaaagct gatgaaaagg atggtatggg caatacctta
    10981 tatattggca gcctcaaaag cgaggtttat ttttgtttgt acgaaaaaga ttatgaacaa
    11041 tatatcaagt tagggattcc attggaccag accgaaacga aaaatcgttt tgagattcgg
    11101 ttgaaaaatg accgtgctta ttatgcgatt caagatttgt taaaaggtcg tagcattgaa
    11161 agtaccacgt tttccattat caatcggtat ttgcgatttg cggataaagt agatggaaaa
    11221 cggcgaacca attggccctt gaatgaacaa tggggtcgtt ttattggacg gaatcggaaa
    11281 gagattcaat taacttctga accgaaaccg tataccattg aacgaacatt aaattggcta
    11341 ggtagacaag tcgcccctac gtggaaaatg gctaaggagt tggatcgatt gaatcaaaca
    11401 acgtatatcc aggatatggt acagaatgcg cgactctcgg accggcataa aaagattttg
    11461 gagcaacaaa gtatggcaat cgaaaacttg attgtatgaa aagaggaatt ttgatggaaa
    11521 ttgtaaaaga tatgttattg gtacttggtg gcagtatgct tggtgttacc gtgatgtgct
    11581 tgatgaatgc aagtgcagca gcggattgag caatggaaaa gaaaggaaag tgacaaaatg
    11641 aatttcggac aaaattt
//
LOCUS       DAGCWU010000081        11657 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.081, whole genome shotgun sequence.
ACCESSION   DAGCWU010000081 DAGCWU010000000
VERSION     DAGCWU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.081"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(413..2422)
                     /gene="metG"
                     /locus_tag="MBZ71_001689"
     CDS             complement(413..2422)
                     /gene="metG"
                     /locus_tag="MBZ71_001689"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBT4429645.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2690..2968
                     /locus_tag="MBZ71_001690"
     CDS             2690..2968
                     /locus_tag="MBZ71_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429646.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            3065..3886
                     /locus_tag="MBZ71_001691"
     CDS             3065..3886
                     /locus_tag="MBZ71_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBT4429647.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4171..5181)
                     /locus_tag="MBZ71_001692"
     CDS             complement(4171..5181)
                     /locus_tag="MBZ71_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4429648.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5193..6146)
                     /locus_tag="MBZ71_001693"
     CDS             complement(5193..6146)
                     /locus_tag="MBZ71_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4429649.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6136..7206)
                     /locus_tag="MBZ71_001694"
     CDS             complement(6136..7206)
                     /locus_tag="MBZ71_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429650.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7212..8255)
                     /locus_tag="MBZ71_001695"
     CDS             complement(7212..8255)
                     /locus_tag="MBZ71_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4429651.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8256..9197)
                     /locus_tag="MBZ71_001696"
     CDS             complement(8256..9197)
                     /locus_tag="MBZ71_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4429652.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9281..9628
                     /locus_tag="MBZ71_001697"
     CDS             9281..9628
                     /locus_tag="MBZ71_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBT4429653.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9698..11359)
                     /locus_tag="MBZ71_001698"
     CDS             complement(9698..11359)
                     /locus_tag="MBZ71_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4429654.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3320 a         2351 c         2104 g         3882 t
ORIGIN      
        1 attttcaatc acacatataa gagcacaaac taattgaaca taaagtgata gaacttataa
       61 ttaacacatc aacgctactg ttagatataa atatagatgt aataaagtca ttgcattcaa
      121 actttatgaa tgtaaggaca ataaaaaaga gtaccagatg aatcaattaa aatctgcccg
      181 accctttatc attatgcttt ttttctgttt ttacgattcc tattagtatt attctaaatt
      241 aacaaaaatc agcgaagctc gtacttcttt ttctaggatt tattttggat agttctctgt
      301 ttctgtatta tatgattggc ttcaagtaat aagaaggaat ttacgaaaat tgcttctcaa
      361 atttcggaaa aagggggtat gacacaacta gtgtcatacc ccctttgaat ttctatgcga
      421 tttctgagcc atttggcatg gattttggtg cttcgattac ttgtaaagag ccatctggtg
      481 cttctgcaga aaggatcatt ccttgactga tttgtccacg catctttcgt ggtttcagat
      541 ttgctacaat gacgactttt ttcccgatca attcgcttgg atcaggataa aattcagcga
      601 ttcctgacaa gatttgacgg tcttggctgt ctccagcatc tagtcggaat tgcagcaact
      661 tatctgcccc ttctactttt tgacagttga tgacttctgc tacttttagt tctacttttt
      721 caaatacatc aaatttgatt tgtttttctt tcgttgaaac aagttctgtt tcttctggat
      781 cccatttgac tgtatcttcg tttgtttgag taccttcaga cattttttct tgaataaaag
      841 caacttcttc ttccatatct aaacgtggga agattggtgt accttttgca actacttttg
      901 ttcctgaagg aaattcgcca aagtgtaatc cttcaagatt cattgtttca gagtccaatc
      961 ctagttgatt gaagatcttc tctggcgttt ctgtcatgat tggctgaagt aatatcgctg
     1021 cgatacgcaa agattcagct aaatggatca tgacactatt caattcagct ttcttttctt
     1081 cgtcttttgc taatacccat ggttctgttt catcaatgta tttgtttgca cgagaaatca
     1141 gcacccagat ttcagccaat gctgtattga attccatttt ttccattgca tcatggtaac
     1201 gtccgacaac gttagcagcc gtcgtagaca gttcactatc aaatggagtg acaagtgagg
     1261 catagtcagg aacgagtcca tcacaatatt tgttgatcat agcaatcgtt cgattcaata
     1321 agttccctag atcatttgcc agatcataat tgacacggga aacaaaatct tctggcgtaa
     1381 agacaccatc tgacccaaac ggaacagcac gaagcaaata ataacggagt gcatctaaac
     1441 cataacgttc cacaagcatt tcaggataaa cgacatttcc ttttgattta gacatttttc
     1501 cgtctttcat caatagccag ccatgcccaa atactttttt aggtagaggc aaatcaaggg
     1561 ccatcagcat gattggccaa tagatcgtat gaaagcgaac gatttcttta ccgaccatct
     1621 gaacatctgc aggccagtat ttttcgaata gactttcatc ttcggagcca tatcctagtg
     1681 cagtaatata attggataat gcatcgatcc atacatacac cacatgtttc ggatcactct
     1741 tgactggtac tccccaagta aaagttgttc gtgaaactgc aagatcctct aatcctggtt
     1801 tgataaagtt gttgatcatt tcgtttttac gagattctgg ctggatgaat tcgggatgtt
     1861 cttcatagta ttccaataga cggtctgcgt atttgctcat gcggaagaag tatgattctt
     1921 cttttaccaa ttctacttcg tgtccacttg gcgctttacc accgatcacg ttaccttcgt
     1981 catctttgta cacttctgca agttgtgttt ctgtaaagaa ttcttcgtca gaaacagagt
     2041 accagccttc atattcacct aagtaaatat ctccctgttc taataaacga tcaaatattt
     2101 tttgaacagc tgctttatgg taatcatctg tcgtgcgaat gaatttatca taactgatat
     2161 caagcgtttt ccataatttc ttgacatcag ccgccatttt atctacatat tcttgcggtt
     2221 ttacacctag ttcttctgct tttgtttcga ttttttgacc atgttcatcc actccagtga
     2281 gataaaacac atcaaagccc atcaaccgtt tataacgagc aattgcgtca catgcaatcg
     2341 tagtatacga gttgccgata tgaagtttgc cgcttggata atagattggc gtagtgatat
     2401 aaaaagtttc cttttctgcc acggggctct tcctccttta tcgcatgatg cgaattcgtt
     2461 tccttgctag tataccataa aaaaagcatg ctgttagctt tttttcaaaa tgaaaaagac
     2521 tggataaacg acttctatct acgttcaatc tatcctttaa aaggatgaat aggttgaata
     2581 aatgcgggtt tttgttttgt ttcatatcta ctaaaaaacg gctttttatt tctcccctca
     2641 cacgtggtat aataaagact atctatctta gtaagggact ggaacaatca tgcagaagaa
     2701 acatcttttt tttacactta gtattgcttt tctttcatta gcacatttaa tattcagcta
     2761 tttttatatt cgtatgtatg gttattttaa cctccatggg catttgaaca gttttatgac
     2821 agcagcttgg attttgcgtt ttatcattga cgtatatatc gttatttgtg gcttctttgc
     2881 cattcgtgaa gagcggtata aagtattgcc gttttatctg cttttctttt tattcaatct
     2941 catacttcca tttatttttc atatttaaat gataacctag atttttcaag taaatccttg
     3001 gttttttttc agagtctgct acaataaggt aaaaagtact tttactgtca atggaggtgg
     3061 tcagatgaac cgttttgctt ctaaagaaga agaaaaatta tattatgaac ataaagcaag
     3121 tgaagaagaa tttcttaact ggtatcaaaa acaggaacgt ccagaatatg acaagccctc
     3181 tttgactgtc gatatcgtac tgatgtgcta taacaaagaa gaagatcagc tgaaagtttt
     3241 gttgatccaa agaaaagggc atccttatcg aaattcatgg gcattgcctg gtggctttgt
     3301 ccaaaaggat gagtcaactg gagaaagcgt cttaagagaa acaaaggaag aaactggtgt
     3361 ggttatttca aaagaaaata ttgagcaact gcatacattc agtacgccta atcgagatcc
     3421 gcgaggctgg gtcgtcacag tcagctactt agcctttatt ggagaagaac cgctaatcgc
     3481 cggagatgat gccaaggaag tacgctggtt cacactagaa cgtcatagta acaagatcca
     3541 tttatctagc ggagaagtgg ctatctcgct tgatttagtc actggagagt cttctggcaa
     3601 agatactttg gcttttgacc atagtcaaat catcttgaaa gcattcaacc gtgtagtcaa
     3661 taaaatggaa cacgagccgc aagttctaca agttttaggt aaagatttca cgattacgga
     3721 agctcgaaaa gtatttgcta aattcttagg gatcgattat aaaacgatcg atcattccaa
     3781 ttttaagaaa gctatgctcc aatatttcga tgagatcggt gaacgtcctg taggaatcgg
     3841 ccgtccttca aaaatctatc agctcaagcc ggggcaccat gaataaggtt gtgagcaaaa
     3901 gattcagacc taatcattca agaaaaaacg caaaaacaac tctttgtgag ttacgttttt
     3961 tgtttcagaa ttttgatgta ttcgtaaact ttctgaataa aaaattaaca atagcagaaa
     4021 aagctcgtac gaactttttc tgctattgtt ttattggttt gtcataatta gtgagctttt
     4081 cttcaaaaga aaaattccta gaaacaaaag tcattaagac tttttattaa caaggctaac
     4141 cctaacgctc ctatcagttt cattcatttt ttatcccttg ataaccgaat gtctgcccat
     4201 tttttctact acttggctct ttcttacgga attgctttct tctgctccgt cggtattgat
     4261 caaaagaaat tttttattat accattggga cttataacgc aattttgtta ttaatttatg
     4321 ccagtcgtga atagaaaaat cagcatgtgc ctcttctttt ggaaaagatt ctacaaataa
     4381 gtagtcatat tgtttgacca atttcccaga taattgttca agataatctt ctcgctggtt
     4441 cttttgtttc agataaagat ccattacttt tctcttttgt ttctgataat tgcttgcctg
     4501 atctaacctg atctttctat ggtgagcgga gcggactttc acattcaatt tccgctgtgc
     4561 atgttgcagc ttttcttctg tctggtgaaa ttttggtaaa gttacttcta gaggtcgaga
     4621 tgtttggatc aatgtcttcg gatgataggc gatcccgatc catttagcta aagattcttt
     4681 ttttactggc gaaacatcac aaagtagtgc gacataaaat tcttcattat atcgagcaga
     4741 aatcgtagcg gagcgaatcg atccttcaac tggccgatgc tgatttatct tgattagttc
     4801 tttttgtttt ggtaacttta agtagccatt ttccagatag atcgtacggg tttggttgtt
     4861 ggtcgtataa gattgccaca tacttttttt cgttttaagc tttgggaagc tggcacgtcc
     4921 cttgaaataa ttgcgaaaag cccgatccaa attaagctgt gcatttgcta aagcaagact
     4981 gtctacttct tttaagaatg ggtattcttt ttttaatgta gcgggtgtga gcgaaaaatt
     5041 ggctactcct gtttgttgcc aacttttctg ccgctttttc agcaaatggt tataggtaaa
     5101 tcgagcacat ccaaaagtac gaatcaattg ctgtttttgt tcttctgtcg gatacatacg
     5161 gaatttaaaa gcttttagca tctatgtccc tcctagttag caaccagaag agattccttt
     5221 tgtcgtttta gtttttttgc gtaatatggg acttcctgct cagttgcata gacaaaatgt
     5281 tctggtgcga tttcacgaag ttgacgtact tgtccgtcat ctggttcagg ttgatatttg
     5341 attcttctgc gttttctctc atggtctgga tctggtagag gaatagcaga taacaaactt
     5401 tctgtataag gatgaacacc gtgattatag atttcatcac ttgttcccat ctctaataac
     5461 aagccgttat gcattacacc gattcggtca ctaatatgtt taaccatcga caaatcatgt
     5521 gcaataaata gataggtcaa ttgatgttct ttttggagat cttgcaacaa attgaccact
     5581 tgggcttgga tcgacacatc tagtgcagaa attggttcat cacaaatgat aaaacgcggt
     5641 tttactgcca atgctcgtgc aataccaata cgctggcgtt gcccacctga aaattcatga
     5701 ggataacgag taccgtggct tggattcaat ccaacagttc tcaatagatc atcgactttt
     5761 tttgcacgct cttcagatga tttagctagt ccattaacat caattccttc ggcaatgata
     5821 tctctgactt tcatacgagg attcaacgaa gcgtatggat cttgaaagat catctgaaca
     5881 tcatgccgga atttcgtcaa acctttttta tctttgatct tcgttatatc ttgaccgtcg
     5941 aataatatct caccatcagt aggttcattc aagcgaatga tcgtacggcc agtagtggat
     6001 tttccgcttc cagattctcc aactaaaccg aatgtttctc cttcataaat gtgaaagctg
     6061 atgtcattta cagcatgtac ttcattcttt tttcctacat tgaaatactg tttcaatcct
     6121 ttaacttcaa gtagtttatt tggcatcagc aattcctcct tgaccgtaag tatgtttttg
     6181 tttctcttta aagatagccc agcgacgttg gatttcttct ggcggtgtca ctttgggtgc
     6241 ctgcggagca agtagccaag ttgctgcctt gtgtgttttt gatagttcaa aaaatggtgg
     6301 ttcttgttct gcatcgattt tcatcgcgaa ttcatttctt ggatagaatg cgtctccttt
     6361 tggcgggttc aataaatccg gtggagaacc aggaatagca tacaatcgat cgttcgcact
     6421 atctaatgtc ggcattgaac caagcagtcc ccaagtatag ggatgttgcg ggttgtagaa
     6481 aatttcttcc gatgttccaa cttcaacgat tcttcctcca tacatgacag cgacacgatc
     6541 agcgacattc gctactacac caaggtcatg agtgataaag ataattgatg tatcgatttt
     6601 tgtctgaatt tctttcagca gctccaaaat ctgtgcttga atagtcacat caagcgctgt
     6661 tgtaggttca tcagcaatca ggatttccgg ataacatacc agtgcaatcg cgatcacgat
     6721 ccgttgacgc tgacctcctg agaactgatg cggataactg ttgatacgtt tctcagcttc
     6781 gggtattcca actaatttaa gcaattctaa tgcagctttt tgtccctctt tttttgaaac
     6841 tttattatgc tttctaaggg actcagcgac ttgcttccca atgggcatcg tcggatctaa
     6901 agaggtcatt ggatcttgga aaatcatcgc tatttcttta ccacggattt tttgcatctc
     6961 tctttctgat tttccaacga tatcgttacc tttaaagagg atttgaccag catctatatt
     7021 ggcgttgctt gacagcaagc gcatgatgct tctggtcgtc actgattttc cacttccaga
     7081 ttccccaacg atcgccaatg tttctccttt attcaaatga aaattgacac cgcgaatcgc
     7141 tcgtaccttt ccagcatacg tatcaaaaga gatttctaag tctttcactt ctaaaatttt
     7201 cgtcatcgtc ttcactcttt cattttagga tcaaaggcat cacgcaatcc atcagcaagt
     7261 aagttaaagc caatcatgat aaccgataaa gttactgccg gaatccataa tagataaggc
     7321 agatacagga atgtcttgta cccttcgctc aacatcgttc ctaatgaagc tgacggaggc
     7381 gtaagaccta aaccgataaa gcttaaaaag gcttcgaaga aaatagcaga tggaatacta
     7441 aacatcattt gaataataat gacactagag atattcggca aaatatgttt gaatgcaatt
     7501 ttcattcttg attcacctaa tgtcaatcca gctaaaacat attcttgatc ttttaatttc
     7561 attgtttgtg cacgaacgat acgtgccatt ggtatccagt ttgtgatggc catagcagcc
     7621 acgattgaaa aaattcctgg ttcaaaaacg accaacatca aaatcatgac aacgaggttt
     7681 ggaattcctg ataagatttc caaaaaacgt tgcatcacgt tatcgacacg tccgcctaac
     7741 aaaccagaaa ccaacccgta agctacacca atcgttacat ctagtaatgc agcaataaat
     7801 gcgatcagca acgagattct cattcccata aataagcggc ttaatacatc acgtcccaaa
     7861 ccgtctgttc ctaaaaagaa atttacgttt gaagggacgt ttgcttgagc atatttatca
     7921 acaagttcac caccaaccat tgcttttcca ttcaatccat cgatattgat tcccggaata
     7981 cgaggaggca aattgatata agccacgttt tgggcagttg gatcatgagg agaaacaaag
     8041 attgtgatca cagagatcaa aatgatgacc aatagtaaaa ccattgaaat aacggcagct
     8101 ttgtttttct ttaaacggcg ccatgaatct tggataaaat tcaaagaagg tgcagtaatc
     8161 tgttcacgtt cttcttcggt atttttttgt aatggaagaa attcatccgc agggatatct
     8221 gcaactgttt cataacgctt tggtatcatt tccatttatc ctcggctccc ttctgaaaca
     8281 cgaattcttg gatcaactag accgtacaat agatccacga tcaaaataac aaaaatcagc
     8341 ataaatgagt aaaggatcgt tacagccatg atcgtaggat aatcatttgt tgtgattgac
     8401 ttaacgaatt gttccccgat accaggaata gcaaagatat tttctaccac cagtgatccg
     8461 gtcatcaatg ctacagctaa cggtcctaat aaagtcatca atggaatcaa actatttcga
     8521 agaccatgtt taaaggcgat ttcccaacgg ctcaatcctt ttgcttttgc taattccaca
     8581 taatcactat gcagtacttc caccatttcc gtacggataa accgagcaga gtcagcaagc
     8641 ggcgacatgg ccaacgcaat tgttggtaag attgtgtaca caaagccatc ccattttgcg
     8701 attggcaaaa cttgtaattt gattgcaaag atatattgta ataaaacagc aaatacaaag
     8761 tttggaatgg aacgacctaa tatcgctacg agtgtagaag atgtatctgc ccatgtattt
     8821 tgcttcatgg cagagatcgt tcctaaaatc gtccctagga ctgttccaaa gataattgcc
     8881 tgtaacccta actgcaagga tggtccgatt cttccagcta acaaatgtgc gactggctgg
     8941 tttttaaatt ggaaggaaat tccaaaatct ccttgaagca aattagaaag ataaatccca
     9001 tattgtacga taactggttt atccaaaccg acttgtttat ttagcatctc gattgtttca
     9061 ggactcaaac gttcctgatt cgtatatggc gtgcccggca gcaattgcat caagaaaaag
     9121 gtgattgttg caattaacca aagcgtgatg atcatgaaaa agacacgttt taatacatat
     9181 ttgatataac tattcactaa tgtttccccc tagcttttga cttctgacag atgtttcgct
     9241 acattattag ataatgaacc aaaaataaag gagcaatcaa atgaaaaaaa aatctatccc
     9301 ctacgcagta gcttttctac tgattctggt tattttaata aaaaatctca tcaaccattc
     9361 ctttacactc attcaattat ctaatgatct attcttatgg tcattgccgt ttctgattat
     9421 cggcggtttt ttatgggtct tttcttcggg tttcttcgat cattttcagc gatcggtcca
     9481 tcttgcacga acaaggaacc gaaagaaaaa accagaattt tcctcgctct cgtctgccag
     9541 ctacggtatg tacagctttt ggctgatcat tgccggaata ctaattgctc tttctgctat
     9601 tttcatgctt ttttctttgt taggataagt gaatatgaga acgaggttgc gacatttgcc
     9661 gcagcctcgt tttttctcat gctcacggta ttaacgatta ttcttcaacg tatgtccatt
     9721 tgtaatccca tgaagcgcct gccgcatggt gaacgatacc tttgattttt tcattcacta
     9781 ggtgaccttc tgctttttgg tacaccggaa cgacacccat atcatcagag atgatcttga
     9841 ttgctttttg gaaatcatcc caacgggctt gttcatcgcc agcgtctgtt gatgaagctg
     9901 ctttaactgc ttcgtcatat tctgcattgc tccagcgacc acggttgtat gaattacctg
     9961 tgacaaacaa atcagtaaag ctgcttgggt ctgcatagtc agcagcccat ccgcctagta
    10021 ctgcgtcaaa atcacccgaa gttgagcggt caagacgtac agagaatgga acaggtgttg
    10081 gtttgactgt tacgccatcc aagttttctt ggattgagtt ttgaacatat tcgattactt
    10141 ttttcgatga atcatcatca gaagccatca aatcaaattc taaagaatca acgcccaatt
    10201 cttttttcgc tttttcccaa tattcttttg ctttatcttt gtcataagaa actaattttc
    10261 cagcttcttt tgcaaagtct tcgttcgttg ttggactttt taccatatct gatgggatca
    10321 atcctgtaga gacaactgat ccgtcaccta gtacttgttt tactaaagct tcacgatcga
    10381 tagaataaga aatagctttt cttaggttca cattgttgaa tggtgaattc ttttcacgtt
    10441 gattgaattc gatatagctt gtccgcgctt ctttaacaga agtataagct ggatcgtttg
    10501 cgttttgctg tgctaactca ccagacaaga tcacgtcatc tgcttggcca tctttgaata
    10561 agttcaatgc agtagaagat tcttttacta cactgacttt gatttcagaa agttttactg
    10621 tatctttgtc ccagtaatga tcatttttct tataagtcca ttctgtatct gttcctggtc
    10681 catcaaaatc ttctaatgtg aatggtccat tataaacagc tttatcactt gcagatgcat
    10741 aggcatcccc attttcttcc actacggatt gtttttgtgg gaaaaatgat gggaaagcaa
    10801 gcagatattg gaaatatggt gtttgtttcg tcaatttgat ttctaattca tagtcaccaa
    10861 ctgctttgat ccctagttct gatttatctt tctttccagc tgtaatgtct tctgcatttt
    10921 caactggcgc atacaggtaa gcatattcag aagctgtagc tgggtctact gttcgttgcc
    10981 agccataaac gtaatccgct gctgtaacag gatcaccatt tgaccatttt gcgtcttctc
    11041 gtaatttgat tttgtaagtt aatccgtctt cactgacttc tgccaactcg ctagctcctg
    11101 ccggctgtgg tttgctgtct tcatcaagac ggtaaattcc ttcataaacg ttattcaatg
    11161 ctgtaaaact gattgtatct gttgcaactg ataagtcagc tgtaggcatt tcttgaggca
    11221 cgactaagtt aaatacgcct cctccatctg ctgaaccgct ttttgctgaa ctactgcttg
    11281 tggctgaact gccgccataa catccagcaa gtacaaggcc gcataccgca accaaaccaa
    11341 atgtaaattt ctttttcatt ttacgtaccc ctttattgga attttcagaa taattaatat
    11401 tttctgaagt tgtttaagag tttaatgaat aattattaaa aaatcaacac ttttttttgt
    11461 ctttttcata aaataatgat tttttcgtga ggtttcaagg aatttttcag aaaaaatagc
    11521 tttgtacaag gttttccatg gtcttccaaa agccattaaa taattttcgt tatattgtct
    11581 attattgcca tacaaagata aaaaagaata aacaaaaaga aactgtgaca aaagtcttgt
    11641 cacagtccct tatctga
//
LOCUS       DAGCWU010000082        11551 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.082, whole genome shotgun sequence.
ACCESSION   DAGCWU010000082 DAGCWU010000000
VERSION     DAGCWU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.082"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1447
                     /locus_tag="MBZ71_001699"
     CDS             <1..1447
                     /locus_tag="MBZ71_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBT4429655.1"
                     /translation="FLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAETAEA
                     VKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGEVQR
                     GIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWIFPM
                     ALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPLVKG
                     ISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFGSAG
                     ERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTFDYI
                     QKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVKDLM
                     AAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGWKHS
                     FFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1523..2683
                     /locus_tag="MBZ71_001700"
     CDS             1523..2683
                     /locus_tag="MBZ71_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBT4429656.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2796..3803
                     /locus_tag="MBZ71_001701"
     CDS             2796..3803
                     /locus_tag="MBZ71_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4429657.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3827..4852
                     /gene="iolG"
                     /locus_tag="MBZ71_001702"
     CDS             3827..4852
                     /gene="iolG"
                     /locus_tag="MBZ71_001702"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HBT4429658.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4869..5759
                     /gene="iolE"
                     /locus_tag="MBZ71_001703"
     CDS             4869..5759
                     /gene="iolE"
                     /locus_tag="MBZ71_001703"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HBT4429659.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5782..7377
                     /locus_tag="MBZ71_001704"
     CDS             5782..7377
                     /locus_tag="MBZ71_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBT4429660.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7393..7707
                     /locus_tag="MBZ71_001705"
     CDS             7393..7707
                     /locus_tag="MBZ71_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBT4429661.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            8168..8893
                     /locus_tag="MBZ71_001706"
     CDS             8168..8893
                     /locus_tag="MBZ71_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBT4429662.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIVHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            9026..9868
                     /locus_tag="MBZ71_001707"
     CDS             9026..9868
                     /locus_tag="MBZ71_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBT4429663.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            9868..10815
                     /locus_tag="MBZ71_001708"
     CDS             9868..10815
                     /locus_tag="MBZ71_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBT4429664.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            10830..11063
                     /locus_tag="MBZ71_001709"
     CDS             10830..11063
                     /locus_tag="MBZ71_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBT4429665.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            11394..>11551
                     /locus_tag="MBZ71_001710"
     CDS             11394..>11551
                     /locus_tag="MBZ71_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4429666.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINA"
BASE COUNT         3659 a         1957 c         2591 g         3344 t
ORIGIN      
        1 atttttaaga aattatattg atggtcgttg ggtcgctagt cgctcaacag agtttgaaga
       61 agtaatgaat ccggctacag aagaagcgct ggcgaatgta ccgttgtcta gcaaagcaga
      121 gacagccgaa gcagttaaag ctgccgatgc tgcgtttcaa acttggaaaa aagttcctgt
      181 tcctgttcgc ggacgttatc tgttcaagct gcatcagctt ttaacagatc ataaaagtga
      241 attggcgcgt ttgatcacgc tggaaaatgg gaaatcattg tccgagtcac tgggagaagt
      301 tcaacgagga atcgaaaatg tggagcacgc agcgggaatc accaatttaa tgatgggaga
      361 ttctttatca acagcggcga ctgatattga gatcacgaat tatcgttacc caatcggagt
      421 aatcggaggg atcacaccgt ttaattttcc aatgatggtg cccttctgga tcttcccgat
      481 ggctctggcg gcaggaaata cggtggtgct gaagccttca gaaaaaacac cattgttgat
      541 ggagaaaatt gttgagctgg ttgaaaaagc aggctttcct agcggtgttt ttaatgttgt
      601 ctatggtgcc cacgatgttg tcaacacatt gctgcaagat ccactggtaa aaggcatcag
      661 ctttgtagga tcaaaaaatg tcggtgaata cgtttaccgt gaaggaacaa aaaatctaaa
      721 acgggtacaa gcgttgactg gagcgaaaaa tcatgttgtt gtgctgaaag atgccaatat
      781 tgatcgggca gtcaaggacc ttattggcgg agcttttggt tctgctggtg aacgctgcat
      841 ggcagcctcc gtcattgttt tggaagagga aatcgcggat cagtttgttg aaaaatttaa
      901 ggctgcggca aaagcgatca agatcggcaa tggcttggac gaaggggtct ttttaggacc
      961 agtgattcga aaagatgcac agcagcggac ctttgactat atccaaaaag gggtcgatga
     1021 aggagcgaaa ctggtactag atggtcgcga aaacattcca gaaaaaggct attttgttgg
     1081 accaacgatt tttgataatg tcacgatgga tatgaagatc tggcaggatg agatttttgc
     1141 cccggtcgca tcaatcgtgc gggtaaaaga tttgatggca gccgttgagc tgacgaatga
     1201 atctgaattt gcgaatggtt cgtgtctgta taccaacaac gcatcagcga ttcgctactt
     1261 cagagaaaat gttgatgctg ggatgctggg ggtcaatctt ggggtgcctg caccgatggc
     1321 ttggtttgcc ttttcaggct ggaagcactc tttctttggt gatttgcatg ccaatggcaa
     1381 agacagtgtt gagttttata cccatcggaa agttgttaca gcacgttatg cgcaaagtga
     1441 gatttaaaga tttgcttcga agctctgtta aagagcttcg aggattctgt tataggctat
     1501 aagccagaga gaggtttttt ctgtgaatat tcaactgaaa atgcctgcca atttaagaat
     1561 tggcggcgga acaatccaat ttcttatgga agaatcagaa caactaggca acaagccgtt
     1621 aatcattagt gatacggtta tggaacagac cgggcatctt caaaagatca aggagctgtt
     1681 gggaaaaaca gatgatcaat tagcggagta tacagacatt caagccgaac cgacgcaaga
     1741 aaatgtttat gatgcgctga agctttttca gaagcatcaa tgtgatcata tcattgcttt
     1801 tggtggcggc agctgtctgg atacagccaa ggtggtagcc gttttggctg ctaatcaatt
     1861 aagtctcgaa gcgattcatg caagaaattt tgatcagctg caagcgggca ttccgctgat
     1921 tgcagtgtca acaactgctg gaactggttc ggaagtaacc gatgtggcag ttattaccaa
     1981 tacaaaaaca gcagtcaaaa tgatgatgaa ggatctcgtt ttcatgccac agatctcaat
     2041 cgttgactct gattttacga aaactgtacc accaaaagta accgcggcaa cgggagtcga
     2101 tgcgctatgt cacgctttgg aatcttatgt ttcagtcaaa gccaatgaag taacgcaact
     2161 attttcactg gaagcgatga agaaaatttt gaacacgctt tctcaggcat atgaaaatag
     2221 cgacgacact gaagccagag aacaaatgag cttagcttca acggaggcgg ggattgcctt
     2281 ttcgaatgcg tcagtcacat tgattcatgg aatgagtcga ccggtggggg cactttttca
     2341 cattcctcat gggatatcaa atgcgatgtt attgccggtc ttttttgaat tctcaaagga
     2401 tgctattcag actgagttag cgacgatcgc cagatttatt tatccggcag ccgaagattt
     2461 atcgctggaa gaggctgcgg attatctgat taaaaaaatc caagagctct gtgagcagct
     2521 gtcgatccca acgattcgag attatggtat tgatcagcaa gcgtttatgg cagccttaga
     2581 caaaatggcc gcggatgcgt tgaccagcgg aagtccgcaa aataataaaa aggtgcctag
     2641 tcatagtcaa attaaggaat tatacgtaca agcttatggg tagtaaaagg cggagcaaag
     2701 agagggctct accttttaag aataaaagct acacaggaat aattttgtaa gcgcatacta
     2761 tgtgtgctta aacaacagaa cagggggata agtaattgag agtaaaagta ggcgttatcg
     2821 gaacaggtgc aatcggcatc gagcatatcg acaggatcaa tcagaaaaca caaggggctt
     2881 atgtgacagc agtctcagat atcaatgtaa aagctgctaa agaggttgcc aaacgtgtcg
     2941 atgctaaatt ctttgaaacc ggagaagcat tgatcgcttc tgaagatgtg gatgtcgttg
     3001 ttgtgacttc ttgggacccg acccatgagc agtatgtgtt agaagcaatc aagcatggga
     3061 aatatgtttt ctgcgaaaag ccattagcga tggatgcagc aggctgccgc cggatcgttg
     3121 atgcggaagt tgcttatggg aagaaattgg ttcaggttgg cttcatgcgc cgctatgacc
     3181 gcggctacat tgaactgaaa gaagcaatcg aatcgaagca atacggagaa gcattaatga
     3241 tgcattgtgc ccatagaaat cctagcgcag atgaaaacta cacaacaccg atggcgatca
     3301 gcaacaccgc gattcatgag attgatgtgc ttcgctggtt gctaaatgaa gattatgaat
     3361 ctgttcaaat gatcttgccg aaaaaaacca gcaactccca tgccgatttg catgatccgc
     3421 agctgctgat ctttcaaaca aaatccggtg taacgattga tttggaaatc tttgtgaatt
     3481 gccgctttgg ctacgatatt aaatgtgatg tggttttcga aacggcagaa gtaggaatga
     3541 cggacccagc ctatacacga attaaatcgg aagaaaaaaa ttacacacct gtttcaccag
     3601 attggcagac ccgctttttg gatgcctatg attatgaatt caagctgtgg atcgattcga
     3661 tcaaaaatga tgcggttgtc ggtcctaccg cttgggatgg gtatgtagca gcgatcacca
     3721 tgatggcttg tcatgagtct cgtgaaacaa aaacaaaagt aatgattcaa atggatgaac
     3781 gaccagcact atacaatgag taattctcag aaaacggagg tatttcatga acaacattag
     3841 agtaggcatc gtcggtttag gaagattagg aaaagtacat gcacaaaatt tagcaacagg
     3901 tgtcagttgt tgtgagctta cggctgcgtg cagtttagtt gaaacagaat tagcattcgc
     3961 acagaagaaa ttaggcgtca agcatacttt ttccagctat gaagaaatgg tcaaaagttc
     4021 cgtgatcgat gcagtcttta ttgtttcacc atcaggcttt cactgtgacc aagtccgctt
     4081 ggcgatggaa aatggcaagc atgtctttac tgaaaaacca ttaggccttg aagttgaaga
     4141 tattaaaaag acccaaaaag tcatcgaaga gcatcctgaa caggtcttca tgttaggctt
     4201 catgcgccgc tatgatgagt cgtatcagta tgcgaagaaa atggtagaag atggcgaaat
     4261 cggcgaaatc acactgattc gttgttatgg gatcgatccg agcgaaggct tggaaagctt
     4321 tgtcaaattt gccatgaata acgacagcgg cggcttgttc ttagatatgg cgatccatga
     4381 tattgatttg gttcgctggt ttaccggcca agaagtcgat caagtgtggg cgattgggaa
     4441 aaatgcggcg tatccagaat tggatcaggc tggtgagctt gaaactggag ccgcaatgat
     4501 gagcctgagc gataaaacaa tagctttact agttgccggc agaaacgcag cccatggcta
     4561 tcatgtcgaa acagagatca tgggaaccaa aggaatgctg cgggtagcgc aagcgcctga
     4621 gaaaaatctt gtgacgatca tgaacgatca agggatcatc cgtccatgtt cacaaaactt
     4681 cccagaacga ttcagagaag ccttcattcg tgaagcccaa gaatttgtct catgtattca
     4741 agaaaaacgt caaccagcgg ttaatgcaga agatggattg caatcaacta agatcgcgtt
     4801 ggcttgtcaa gaatcattcg atacgaagag tttagtacaa ctaaaaaaat aagcaggagt
     4861 gtgagaatat gaccaaacaa attcaattag ccatcgcacc aatcgcgtgg accaatgatg
     4921 atatgcccga gcttggtagt gaaaatacct ttgaacaatg tatcagtgaa atggcccttg
     4981 ctgggttcac cggaacagag attggcaaca aatatccaaa agatccagag gtgttggaac
     5041 aatatttgaa tattcgcggc ttaaaggttg ccagtgcatg gttcagtgcg tttctgacaa
     5101 ctaaaccttt tgaagaaact gccgaagcct tcaaagcaca tcgggatttc ttacatgcga
     5161 tgggcgctaa agtcatcgta gtagctgagc agggtcatag tatccaaggg attatgacag
     5221 cgccagtctt tgatgataaa ccagttttca ctgatgaaga gtggaaaaaa ctggcggaag
     5281 gattagaaaa attaggggat ttggcccacg aaaaggatat ggagatcgtt taccatcatc
     5341 acatgggtac cggcgttcaa acgacggaag aaatcgatcg tttaatggaa atgactgatc
     5401 cagacaaagt taaattattg ttcgatacgg gacatttggt cttttctggt gaaaatccaa
     5461 tcgcggttta caaccgtcat caagatcgta tcaaacacat ccatttcaaa gatattcgtc
     5521 aagaaacggc tgaagaagta gaaaaggaaa aagacagctt cttaaacggc gtgaagaaag
     5581 gcgtcttcac ggttcctggt gatgggatga ttgatttcac tccaatctgg acagcaatcc
     5641 atgccagcga ctatgaaggc tggatcgtag ttgaagcaga acaggacccg gctaaagcca
     5701 atccatttga atatgcagtc aaagccagaa gctacattcg agaggtcacc ggtctttaat
     5761 ctattgaaaa ggagctagac gatgaatctt ttttctataa tcagtttcct agtgattgtc
     5821 atctgtgtat ggttatttgc ctattctaga tccagaagtg tcgatacttc tggatcagaa
     5881 gggttcttca tgggagggcg gagcttaact gctttgccaa tcgccggcac gatcatcatg
     5941 acgaacctgt ccaccgaaca aatagtcgga caaaatggtc aaagttatca tgcagggatg
     6001 gaagtaatgg cctgggaagt aactgctacg attgcgatcg tagctttggc tgtgatattc
     6061 ttgccaaaat attttaaata cggcatcaat acaatctcag atttcatcga gattcgttat
     6121 gatacggtta ccaaacgaat tatttcgatc ttatttattg tgacctatat gacttctttc
     6181 ttacccgtgg ttctttattc tggatcgctg gtgttcaata agatcttcca tattgatgag
     6241 ctgctgggcg tccagccgat cgtcgcgatc ataattgtag cgatggcaat tggtattgtc
     6301 ggcatcctct atctgctgat cggcggcttg tcgttaagtg ccaatagtga ctccatttat
     6361 ggagtcggtc tgctggcttg tggtctgctg gtcccaattt taggcttaat gaagcttggt
     6421 gacggcagct ttgttggcgg gatcgaacac gttgtggtaa acacgccatg gctgcttaac
     6481 tctgtgggat cgatcgactc ggctgttgtt ccatggccga cactatttac agggatgctg
     6541 tttaacaatc tgtatttctg gtgtaccaat caaatgatcg tccaaaaagc attgtccggc
     6601 aagaatttag ctgaggcaca aaagggcgct ttcctagtcg gcgtcttcaa aatcttaggt
     6661 gcgttattcc ttgtctttcc agggatcgtc gctcgcaata tttttggcga tagtttaatg
     6721 tcaaatccag ataacgcgta tcctttttta gtcagtgaag tattgcctaa aatgatcttt
     6781 ggtattttcg ccgctgttat ctttggcgcg atcttatcat catttgcggg agctttgaac
     6841 gcgacatcga ccttgttttc actggatttt tataaaccgc ttatcaataa gaaagcaaat
     6901 gataagcaaa tcgctcgtgc tggtaaaaca gtaacggtcg ttgtggggat tatctccatt
     6961 atcgtagcgc cattcatttc ttttgcaccg gctggattgt atcagttcgt tcaagaattc
     7021 aatggactat acaacatgcc gctgctcgtg atcattctct ttgcctttta ttcaaaaaaa
     7081 gcaactgctt ttggggcaaa aacgacgatt gccgtgcata tcgtcttgta tgcactatca
     7141 aaaatgcttt tccaaaatat ccatttcctt tatgttttga cggcattatt cgttctggac
     7201 attatcattt tgatggtggt ttccaaattg aaacctgatg gagaatttgt gttgaactca
     7261 ttcgatacaa aggtcgatat cgagccttgg aaatacacca aggtgactgc tatagcattg
     7321 gtagtcattg ttattttaac ttatgtggca ttttcaccaa ttggctttgc aggctaaaaa
     7381 caggagggta ttatgcataa ggatctattg attatttgcg agaccggaat cagtgcgtcg
     7441 ctgctcgtat caaaaatgct tgaaacgatc agagccaaaa atctatcgta tgagatagat
     7501 tatgcaacga tccgaaacgt tgaagaaaaa ctgaattata aagactatga cgttttactt
     7561 ttaacgccgc aggtcgcacg ttctgaggaa aaagtaaaag aaatcatcaa aaaggaaaaa
     7621 tgtaccgctc agatcgttta tatcaaacaa gaagattttc gttatatgaa tattgaaaac
     7681 atcattgcaa gcatcgaaga agattaacga gaatctttga ttattgagtg tcgaggaggt
     7741 ggtatatgaa aagatggcat gttcgccccg atcaccccaa tatgaagggc ggacttatta
     7801 gaaataccca atcttgtatt tattcaatag ggtaagcgga cctcaatagc cagctcatcc
     7861 ccaaaaagtc gaatggagac acttaaatga gtgtacctat tcgacttttt gagactctaa
     7921 gtcaaatcgt gttgttctca ttaaatcact gaaaggaaat gcttcggcgt ttcctttttt
     7981 ctttttgttt ttcttcgtgt tttgaaaatt acttactatt tactatacga gtagtttcaa
     8041 ataattttga catcctaatc ttcaacaaca gaaaaaaatc taaattataa aatttagggt
     8101 tgactaaaat ataatttttg tataccatat gatcgtgata atcattatca tttagaagga
     8161 gaaagttatg ttagaagtga aaaagcttaa tgtttgttat aacgattttt tagcattgaa
     8221 cgagatttct ttaaaaatac aagaaggtgc tttaacaggt attattggtc caaatggtgc
     8281 tggaaaatct acattgctta aagcaatgtt aaatattatc cctcatcagg gagaggtctc
     8341 cattaataat gaagatatca ataaaaaact ggtgaagatt gcatatgtag agcaaaaagc
     8401 tgatattgat tttacttttc caataaaagt aaaagaatgt gtatctatgg gaacatatgc
     8461 cggaatgaaa gtttttcaaa gaattaaaaa tgcagaatgg caaagagttt caaaggcttt
     8521 ggaaaaggtc gatatgggaa aatattctaa tcatcaaata ggagaattat ctggaggaca
     8581 atttcaacgt gtacttttag caagatgtct tgctcaaaat gcggatttca tatttttaga
     8641 tgaaccattt gtagggatag acttagttag tgaacgaata attatggata cgttaaaaga
     8701 attgaaacat cagggaaaaa cagtgctaat tgtgcatcat gatttaagca aggtaaagaa
     8761 gtattttgat aatattattc ttttgaatcg aaatctaatt gttcacggaa gtgtagaatc
     8821 tgtatttaat gaagagaatt taaaaaaagc ctatggagat actatattta ttggagaggg
     8881 gggaacggct tagcctctca atccaataaa tatattatct caaaagaaat gagttaatac
     8941 caaattaaaa taacgatggt tcatagggtt atccaatcaa aaccggaagg ttaaaaccct
     9001 aactctaata tacaagggga ataatatgct tcaaaatttt attaatggtc tgtatgactt
     9061 tcacttttta cagaatgctc taattacatc tgtagttata gggattgttt ctggaacagt
     9121 aggttgtttt attattttaa gaggaatgtc tcttatgggt gatgctattt ctcatgctgt
     9181 tttgccagga gtagcaattt cttatatttt aggtattaat ttttttattg gtgcgattgt
     9241 atttggtcta ttagcttcta tcattattac ttttataaaa agcaatagtg taataaaagg
     9301 agatacagca attggtatta ctttcagttc ttttttagca ttaggagtta tccttatagg
     9361 agttgcaaat agctcaacgg atttatttca tatcttattt ggaaatattt tagctgtaca
     9421 agatattgat aagtggatta caattggcgt ttctgtttta gtgttggtta cagttgttct
     9481 attttttaaa gaattattaa tcacttcttt tgatccatta atggcaaaag caatggggat
     9541 gaatgtcagt ttttatcatt atctattaat gattctatta actttagtag cagtaacagc
     9601 tatgcaaagt gtgggaacta tattaattgt tgcaatgtta attacaccag ctgctacagc
     9661 atatttatat gcaaacagtt taaaaactat gatatttatt tcggcgacag tcggagcgtg
     9721 ctcatcattg ctaggtttgt tcattggtta tagttttaac atagcagcag gatcaagtat
     9781 tgttttaaca gcggcattct tatttgtaat tagttttcta atctcaccaa aacaaaaatt
     9841 tttaagaaag aaggagagaa agttatgatg agaaaatgga aagcagtact gggaagtctg
     9901 gggattttga ttgctctttt tatattcggg gcatgttcaa caaatagtaa agacaaagat
     9961 acagtggctt cgaacgaaaa attaaaggta gtagttacta attcgatttt agcagatatt
    10021 actgaaaata tagcaaaaga taaaattgat ttacacagta tcgtacctat tgggaaagat
    10081 ccccacgaat atgaaccttt gcctgaagat gttcaaaaaa cttcaaaagc agatttgatt
    10141 ttttataacg gtgttaactt ggaaactgga ggaaatgctt ggtttacaaa attagtaaaa
    10201 aatgcgaaca aagaggaaaa caaagactat tttgcagcaa gtgatggcat agatgttatt
    10261 tacttagagg gtcagagtga gaaagggaag gaagatcccc atgcttggtt aaatttagaa
    10321 aacggtatta tttacgctaa aaatattgaa aaacagttag cggaaaaaga tcctgataat
    10381 aaaaaattct ataaagaaaa tctagataag tatattgaaa aattggattc tctagacaaa
    10441 gaagctaaat ctaaatttgc ttcaattccg aatgataaaa aaatgattgt tacaagtgaa
    10501 ggatgcttta aatatttctc gaaagcgtat aatgtgcctt ctgcttacat ttgggaaatc
    10561 aacactgaag aagaaggaac accagatcaa ataaaacact tagttgaaaa attacgcaca
    10621 acaaaagttc cctccttatt cgtagaaagc agtgtggacg atagaccaat gaaaacagta
    10681 tcaaaagata ccaatattcc tatctattca acgattttta ctgattcaat tgcagaaaaa
    10741 ggacaagatg gtgatagtta ctatgcgatg atgaaatgga acctggataa aattgctgaa
    10801 ggcctttcga aataatttgg agggtaggta tggtacaaat taaaacaact actatactaa
    10861 tttatttact agcaattcag gaattatcaa aaagaaaaaa gaaattaaaa aataatgatt
    10921 tagcaaaagt tctaagtgtt agtccagcat ctgtaagtga gatgctgact aaacttagat
    10981 atgaggacta tttagatata ggatttcaat taactgataa agggaaaaaa tttattgacg
    11041 attacaaaaa aggttttaag taaaaggctt tatttttagg gaggataaga aaatggctat
    11101 tggagtgatt atgataggac ttatcctaat agctggatat aattttttta aatctttctc
    11161 ggattatttt agaaaaggtg aatgatttag atgagtctat taattcttgt aggattgctt
    11221 tttgtatcag ttattttgct ctttctcatt agtttagctc taaggaatga gttcagtaat
    11281 agaggttaac gattgaaact tcaagtcctg tgtaagataa tatagttaat tcatcttatt
    11341 aggaggaagt acccatagaa accagagatt atattacaac tcaccaaaaa ttaatagaaa
    11401 gtggactaag ccattttgaa aaagtaggat ttcagcaaac taagataagg caactgtgct
    11461 tgaccgcagg cgtaactacg ggtgcttttt ataaacattt tacttctaag gaagcgctat
    11521 ttgaagaaat tattcagccg catattaatg c
//
LOCUS       DAGCWU010000083        11526 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.083, whole genome shotgun sequence.
ACCESSION   DAGCWU010000083 DAGCWU010000000
VERSION     DAGCWU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.083"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(222..707)
                     /locus_tag="MBZ71_001711"
     CDS             complement(222..707)
                     /locus_tag="MBZ71_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBT4429667.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    797..1142
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1187..1828
                     /locus_tag="MBZ71_001712"
     CDS             1187..1828
                     /locus_tag="MBZ71_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4429668.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1847..2482
                     /locus_tag="MBZ71_001713"
     CDS             1847..2482
                     /locus_tag="MBZ71_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4429669.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2500..3315
                     /locus_tag="MBZ71_001714"
     CDS             2500..3315
                     /locus_tag="MBZ71_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4429670.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3634..5070
                     /locus_tag="MBZ71_001715"
     CDS             3634..5070
                     /locus_tag="MBZ71_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4429671.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5259..7706
                     /locus_tag="MBZ71_001716"
     CDS             5259..7706
                     /locus_tag="MBZ71_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBT4429672.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7936..9003
                     /gene="rlmN"
                     /locus_tag="MBZ71_001717"
     CDS             7936..9003
                     /gene="rlmN"
                     /locus_tag="MBZ71_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBT4429673.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9196..10299)
                     /locus_tag="MBZ71_001718"
     CDS             complement(9196..10299)
                     /locus_tag="MBZ71_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4429674.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10852..11400
                     /gene="aac(6')"
                     /locus_tag="MBZ71_001719"
     CDS             10852..11400
                     /gene="aac(6')"
                     /locus_tag="MBZ71_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBT4429675.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3824 a         1948 c         2485 g         3269 t
ORIGIN      
        1 ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca
       61 ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact
      121 agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga
      181 aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact
      241 ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta
      301 gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc
      361 acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct
      421 ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt
      481 aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag
      541 atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa
      601 aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc
      661 agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct
      721 ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt
      781 acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag
      841 agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt
      901 tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg
      961 accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta
     1021 acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg
     1081 tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct
     1141 tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat
     1201 tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac
     1261 cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa
     1321 gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt
     1381 gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga
     1441 agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg
     1501 agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc
     1561 ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt
     1621 agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg
     1681 tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct
     1741 aggcgtggca atcatctatc ttacattgac tggtattcta acaatcgtaa tgaaacaagt
     1801 tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa
     1861 gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc
     1921 agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg
     1981 tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt
     2041 caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca
     2101 gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa
     2161 acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag
     2221 tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagcgat
     2281 tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt
     2341 agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt
     2401 aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact
     2461 aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta
     2521 ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca
     2581 gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt
     2641 gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc
     2701 aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg
     2761 aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg
     2821 ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg
     2881 atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt
     2941 gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag
     3001 atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg
     3061 aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa
     3121 caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt
     3181 gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat
     3241 caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt
     3301 acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc
     3361 gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa
     3421 cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa
     3481 atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac
     3541 aacctccttt acatattttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg
     3601 aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt
     3661 ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa
     3721 gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt
     3781 aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct
     3841 ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt
     3901 cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac
     3961 gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa
     4021 gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat
     4081 tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat
     4141 gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca
     4201 gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc
     4261 aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca
     4321 aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg
     4381 atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg
     4441 ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa
     4501 tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat
     4561 ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa
     4621 gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat
     4681 tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt
     4741 taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa
     4801 gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc
     4861 aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat
     4921 ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac
     4981 gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa
     5041 ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct
     5101 ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata
     5161 agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt
     5221 ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta
     5281 ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc
     5341 tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag
     5401 gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa
     5461 tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt
     5521 caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat
     5581 tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga
     5641 catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg
     5701 ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt
     5761 atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct
     5821 atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg
     5881 atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag
     5941 aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat
     6001 atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac
     6061 cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata
     6121 tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag
     6181 aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag
     6241 cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa
     6301 ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa
     6361 ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca
     6421 caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag
     6481 atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta
     6541 aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa
     6601 tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg
     6661 tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt
     6721 cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg
     6781 ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt
     6841 acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag
     6901 ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag
     6961 taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga
     7021 aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg
     7081 tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg
     7141 ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca
     7201 aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga
     7261 tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa
     7321 tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac
     7381 gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga
     7441 cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag
     7501 caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc
     7561 ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg
     7621 gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa
     7681 cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac
     7741 caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct
     7801 aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat
     7861 ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa
     7921 aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc
     7981 aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg
     8041 tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa
     8101 aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg
     8161 gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg
     8221 atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc
     8281 tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt
     8341 gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt
     8401 tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac
     8461 tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt
     8521 tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat
     8581 ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac
     8641 cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac
     8701 cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg
     8761 caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca
     8821 tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga
     8881 ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca
     8941 gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa
     9001 taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga
     9061 actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac
     9121 aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg
     9181 ggttttagca attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg
     9241 ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac
     9301 atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac
     9361 attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat
     9421 tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac
     9481 agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga
     9541 gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac
     9601 aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt
     9661 caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc
     9721 taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt
     9781 ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc
     9841 tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc
     9901 ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg
     9961 aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg
    10021 gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg
    10081 ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc
    10141 ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat
    10201 cagcactact tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt
    10261 tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg
    10321 ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga
    10381 ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa
    10441 taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt
    10501 tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa
    10561 attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa
    10621 gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt
    10681 cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag
    10741 ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt
    10801 tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc
    10861 agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg
    10921 acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca
    10981 gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc
    11041 cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga
    11101 aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga
    11161 ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg
    11221 gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg
    11281 tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc
    11341 tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa
    11401 taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat
    11461 tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc
    11521 gtgtac
//
LOCUS       DAGCWU010000084        11452 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.084, whole genome shotgun sequence.
ACCESSION   DAGCWU010000084 DAGCWU010000000
VERSION     DAGCWU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.084"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(31..2106)
                     /locus_tag="MBZ71_001720"
     CDS             complement(31..2106)
                     /locus_tag="MBZ71_001720"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBT4429676.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKITNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLNQIAAMALSL
                     ASLDQIITK"
     gene            complement(2108..3025)
                     /gene="glyQ"
                     /locus_tag="MBZ71_001721"
     CDS             complement(2108..3025)
                     /gene="glyQ"
                     /locus_tag="MBZ71_001721"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBT4429677.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3409..4221)
                     /gene="recO"
                     /locus_tag="MBZ71_001722"
     CDS             complement(3409..4221)
                     /gene="recO"
                     /locus_tag="MBZ71_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBT4429678.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4364..5263)
                     /gene="era"
                     /locus_tag="MBZ71_001723"
     CDS             complement(4364..5263)
                     /gene="era"
                     /locus_tag="MBZ71_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBT4429679.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5278..5673)
                     /locus_tag="MBZ71_001724"
     CDS             complement(5278..5673)
                     /locus_tag="MBZ71_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBT4429680.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5657..6133)
                     /gene="ybeY"
                     /locus_tag="MBZ71_001725"
     CDS             complement(5657..6133)
                     /gene="ybeY"
                     /locus_tag="MBZ71_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBT4429681.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6150..8342)
                     /locus_tag="MBZ71_001726"
     CDS             complement(6150..8342)
                     /locus_tag="MBZ71_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBT4429682.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8366..9337)
                     /locus_tag="MBZ71_001727"
     CDS             complement(8366..9337)
                     /locus_tag="MBZ71_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBT4429683.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10043..11422
                     /gene="brnQ"
                     /locus_tag="MBZ71_001728"
     CDS             10043..11422
                     /gene="brnQ"
                     /locus_tag="MBZ71_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBT4429684.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3074 a         2417 c         2102 g         3859 t
ORIGIN      
        1 ctgttctctg gtttatttac atattagaaa ttattttgtg atgatttgat ctaaacttgc
       61 tagtgacaaa gccatggcag caatttgatt taattgcgtt aatcggttct tacgtactgc
      121 tggttcttct gccatgacca tcgtctgatc aaaataattt tcgatcagtg ggcgaaggct
      181 tacaagctga tcataatttt cgttgatcgt ttggtcagca aagcttgttt tcattgcctc
      241 tactgctttg tgcagttctt tctccgcttc gttttcgaac agttcagggt ctacttctga
      301 ttgtttttca agctccgctt ttttcgctaa gttcacgaca cgtgtcaaag cttccatcga
      361 tggtttgaag tcttcatctg caagatgctg cttgaacaat tgtgctgctg caaatacttt
      421 tgtcagatct ttttgttctg cgttcaaaac agcttcgatg acatcatgac gaatattctt
      481 cgttgtcaat aattggcgca gacggccttt gataaagtcg atcacctgtt gttgtcccgc
      541 tgacaagcca atgccaaaac gatccgtatc tttactgata acttcactaa tcgtttcttg
      601 cagaacagac aaaggaaagg cccatttttt cgattcgatg attcgaacaa caccatatgc
      661 ttgacggcgc aaagcatatg gatcgtttga gccacttggg atcatcccaa ctgcaaagaa
      721 agaaaagata ctatctaatt tatcagcaat cgctaaaaca gaacctactg tagtctcagg
      781 tagatctcct tcactggata taggcatgta atgttcacgg atcgctgttg ctacggctgg
      841 tttctcgcct ttaagcagag catatttttc tcccatgatc ccttgtagtt cagggaattc
      901 tcctaccatg ttggttacta gatcaaattt atagatttca gcagcgcgtt gcagatccat
      961 caattcttcc tcagatagtc ctacttgttc gcctatgatg cgagcgatga cttgtacccg
     1021 ttgcattttc tcgtagatgc taccgatttt ttcatggaaa gtaacagttt ttaatttttc
     1081 aacgtattgg tcgattgaag ctttcttgtc ttcattataa aagaattccg catcttctaa
     1141 acgagcagtc aatacttttt gattgcctcg taccacattt tccaatttta cccgatcgcc
     1201 attacgagca gaaataaaat gaggaagcaa cataccttga tcgttccgta cttcgaaata
     1261 acgttgatgt tctttcatag aagtgactag tacttcttca ggaacagata gatatttttc
     1321 ttcgaagtcg ccagaaaaga cagttggata ttcgactaga ttgttgactt cttctagtaa
     1381 atcttcatct agatcaatga tccagtgatt tttttctgct aattcgtttg cttgtgtgat
     1441 gatcatttct tttctttttt gcggtgaagc aataacaaat tgttcattta atttttcttc
     1501 gtattctttt gcatgcgtaa atgtcacatc ttcacctaag aaacgatgac ctcttgaagc
     1561 tctgtctgtt ttgatatcca agatctcaaa cgggattact tcgtcatcta acaatgccac
     1621 gatccagtga atagggcgga tatattcaaa atcgtattct gcccaatgca tcgttactgg
     1681 gaaagtgagg cttttaatga catctgctaa acctgtcaat acttcttttg cttctttgcc
     1741 gtgagtgtat ttcgttacat agacatattc cacgcctttt aattctctaa aagtgatagc
     1801 atctgtcgtc agaccttgtc cgcggacaaa tccttcagct gctttcgtcc agtttccatc
     1861 tgcgtccaaa gcaatttttt tggctggtcc tttgacttct tcttcactgt ctgcttgacg
     1921 ttctggaatg tttgttattt tgattgctaa gcggcgcggt gtagaaaacg tttcgatcgt
     1981 atcataagtc aattggtttt catctaaaaa ttttgctgtt ttttcttcta gttgtttcat
     2041 gcttggtgtg acaacatgag caggcatttc ttccagcccg atttcaagta ataagtcttt
     2101 tgccatctta ttcagcctcc ttcatgtttt ctggatttgc tgtgttttta ttcaataaag
     2161 ggaatcctaa tttttcgcgt tctgccacaa agattttggc tacagcacga gccatgttgc
     2221 ggatacggga aagataacct gcacgctctg ttacggatac tgcaccgcga gcatctaata
     2281 agttgaatgt atggctgcat ttcaaaatat aatcatacgc tggatgaaca agattttcat
     2341 cgatacagcg tttagcttct ttttcaaaac ggtcaaaatt gcttagtagc aagtcttggt
     2401 cgcttacttc aaaagaatat ttggaatgtt catattctgg ttgtttgaag atctcgccat
     2461 atttcacacc atcagcccat tccagatcat agacactttc aacttcttga atatatgaag
     2521 caagacgttc tagtccataa gtaatctcag aagtaactgg tttacaaggt agtcctccga
     2581 cttgctggaa gtacgtgaat tgtgtgatct ccatcccatc aagccatact tcccagccca
     2641 caccggcaca acccatcgat gggttctccc agttgtcttc aacaaaacga atatcgtgtt
     2701 ctaatggatc aattcctaaa agacgcaagc tttctaaata aagctcttgg atattttcag
     2761 gtgaaggttt cattacgact tgaaattgat gatgctgata caaacggttt gggttttcgc
     2821 cataacgccc gtcagctggt cggcgggatg gctctacata agcagcattc catggttccg
     2881 gtccgattgc ccgtaagaat gtatatgggc tcatcgtacc tgctcctttt tctgtatcat
     2941 aagcttgcat cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt aaaatcatat
     3001 cttggacggt caatttttta ctcatctgtt ttgccccttt caatttttaa gcgttgctgt
     3061 caataagaat acaggaaaga aaacgacaaa aagtccctat gtctgattac agacataggg
     3121 acgaataatt tcgcggttcc accctacttc cgaataaaga ttcggcagct tcatttatcg
     3181 tactcgcaag tgcctcttca aaaatcgttt gggtccggct tccactctcc cgaactcgct
     3241 aaaccacgtg ttttttactg atcttggtca atgtacgtat tcattttgca gttttcttgt
     3301 ttatataata gatcctctca gtgaacgatc tatttaatct acggttaata atagacggaa
     3361 gtccctgtat ttgtcaaata gttttgattt ttttgttgta tctttcattt attcttcttt
     3421 ttcaggtatc tctttttcag gcatattttt ctctggcgtc tttaatacgt gtgcccaaga
     3481 cttcatttga tcaatgaact ttttgctttt tagatgaata ccgacatatt catcatacaa
     3541 ctggtcaatg aggtcacgga ttgcctgctt cgtttcttct ttcaagtgga ttccactgat
     3601 cttatcataa gaaatagccg aaaacattcg aacgaaataa acagcgcgag gatctgcatg
     3661 ataacggtgt tcgtccattg gccagtgttt ctcgcatatc acaccgccat atttggaaga
     3721 atagtcaaat tttccttttg tttccccgca tacgacacag tgcgtccaaa ttggcgcgat
     3781 tccgaagcgt tgcagtattt gaacttcaaa gatattcgtg atgatttctg catcatctcc
     3841 ttgatccatc cgctgcagag cttgttgcgt aaattggaat aaatgcgggt catattgatg
     3901 atcttcgatt gctgcatcta ctagatttag caaataggta ccataaccgc tcaaaaagat
     3961 atcttcttgg atgttccgaa acgcatggac gtctttgaca ctgcttaaaa acgacaaacc
     4021 ttcacttcga aaattaccga tatagactgc ttcggtaaag gggagcaaag ccggagctaa
     4081 tggctgattt tgccgatgga ttcctttgat ataaaacatc attttgccag aagattccgt
     4141 aaagattttg actaatttat ctttttcttt gtaattacgg ctagataaaa tcaagccttt
     4201 cgattctgca agctggctca tattctccct cctaaaaaat aaatctggaa aagcagattc
     4261 tgccgatcca gattatttat gtttcgatct tccgccgttt ttctcaacgt gcggattttt
     4321 atccagttta tttctatact taacctataa gaagttcaga atatcagtat tcgtcttttc
     4381 ggtagccaaa gtcttgcaga tagacttttt tgtctcgcca gtctttttgg actttgaccc
     4441 aaagttctag aaatactttg tctcctaaaa gattttcgat atcctttctt gctttcgttc
     4501 ctacttgctt cagcattttt ccgcctttgc cgataatgat tcctttttgg ctatctcttt
     4561 cgacgatgat cgtggcctgg atatggattt tatcattctc gtttcgcttc atcgaatcaa
     4621 caacgactgc gacagaatga ggcacttcgt ctcgtgttaa aaacaagact ttctcacgga
     4681 tcaattcaga aacgataaag tattcaggat gatcagtcac ttgatcatct ggaaaatatt
     4741 gtgggccctc tggcatttca ctgactaata catccattaa gcgttcgaca ttgtttcctt
     4801 ctgtagcaga gatcggaatc acttgtgcaa aaggcatctg cgtcgaataa tcttcgatga
     4861 ttcccagcaa ctcgtctgga tggatcttat cgattttatt gataatcaga taaactggag
     4921 aatgcacgtt ttttagacgt tcaataataa aatcgtctcc ccggccgcgt ttttgatcag
     4981 cactgatcat aaataagact gcatccactt ctttcaacgc actgtaagct gcttctacca
     5041 taaaatctcc tagacgatgc tttggtttat ggattcctgg tgtatcgata aaaatcagct
     5101 gagcttctgg tgtagtatat actccttgga ttttatttct agttgtctgt gccttgtcac
     5161 tcataatggc aattttttga cccacgattc ggttcaataa tgtggatttt cctacattcg
     5221 ggcggccgac gattgcgaca aaccctgatt tatgttctgg catatacgtt ctccttttta
     5281 attaaacaac aaatgatata ttttagggac aaataaaatc aacccaatga tgacagcaaa
     5341 acaagcggtc actaaaactg ctccagctgc catatctttg atttttttcc caatcggatg
     5401 aaaatgaaaa tccgtaaaca tgtccactac attttcaaaa acagtattga tgatctctac
     5461 tatccaaacg agaaaaacag ctaaaagcag ccataaccac tctatccgat caagctggaa
     5521 aatcacgccc ataaataaag caaggatacc gaacgcaaca tgtttcctca tattgcgttc
     5581 ttcctcaaaa acggttttga ctccttggac agcaaattcc acagacatca ggaaatgttt
     5641 gtttttctct acgtttttat cttttgagtc cataggcatc caagatctct ttctgtaaac
     5701 caaacatttc tttctcatct tctggtgtca tatggtcata cccgttgata tgcaagaatc
     5761 catgtaccgc taaaaagcca agttcccgat catatgaatg gccgtaagct tctgcttgtt
     5821 cagcagcacg ttcaatagaa atcatgatgt ctcctagtac acgaggcaat ggattctctc
     5881 catcttcaaa aatcactggg atttcgtctt cgccttcttc ttccagagca aaactgatga
     5941 cgtctgtcgg tgcatctttt ccacggtaat cacgattgat tacttggatt gccgcattgt
     6001 ccatgaaagt aacagacatt tctgtttcct ctggcagctt taaataagat gctgcaaatt
     6061 gcagcaagtc atcgatttct ttcattttct cttctgagac ttggtttgtt tcatccataa
     6121 aggtaatatc cattatacat cgcttctttc tattcttctt gttcttcctt tatcttctca
     6181 acttctgttt tcattttagg atatttgatc cgagaatgga aggtgctact taaaccgctg
     6241 atcaatgatt tttccacttt tcggatttct ttcaaggtca gtccactatc gtctaattga
     6301 ccatcggaaa tacgctcttc gataagatta tggacaaaac tttcgatttt ttcgctcgtc
     6361 ggatgatcca ttgcccgtac tgcagcctca caactgtcag caatattgac gatccctgct
     6421 tcacgacttt gcggttttgg tccaggataa cggaattgct cttctgttac ttccggattg
     6481 cgttctttcg ctttgatgta gaaataacgc attagcgtgg ttccatgatg ctgtttgcaa
     6541 atatcgatca ccatttgcgg catattataa tcttccaata ttttggctcc atctgtcaca
     6601 tgtccaaaga taatatgttt actgtcttct ggcaataaga aattgtgcgg gttttccgct
     6661 cctgaaggca agttctcaac gaaaaagcta gcatgtttga tcttgccgat atcatgataa
     6721 tagcaagcta ctcgagttag caaagaacgt ccgccgattt ctgctacagc gttagcactt
     6781 agattagcta ccatcatact atgatgataa gtacctggcg cttcttccag caactgtttc
     6841 aataaaggat gatttggatt gctgagttca tttaatacaa ttacactgtc atcattgacc
     6901 atcaattcaa tatatgggtg tagaccgatc gtcagcaaga atgagaagag tgcaccagtg
     6961 aacccgcaga caagcattaa ccaagtcgtt gcatcaccaa agctcattcc ttgatagacg
     7021 atcaaaatga catccatcat cacaggaaaa ataatgaccc acatggcagc agataatcct
     7081 tgttctgaga tccgtttgcg tctgacaatc gttgccatga gcccagagaa aacataagac
     7141 atcaagataa tcgttagaaa gttcgtgcca atcgaatcat aaaaaatgaa aagtgcagat
     7201 actgcttgga aaataccagc catgatccct gctcttcggt tcaagaaaaa gcttaaaaca
     7261 agtggtgcaa aagcagcggg ataaaacagc gggatataag tagcttgttc cgtttggaat
     7321 aattggaaaa atttcatcag cactatgctg actgacatag ctgtcacata gaaaagcacg
     7381 tattttgttc tttctcctgc attttggaat tgaagtgaat tataaatcag tacgccgatt
     7441 tgaagcaaaa cagctaaaac catggcaatt agcggaaaaa tcgaagttgt ttgattagtc
     7501 agtcctagta attccaattt tttcatagct gaagcatcga tttgattacc ttcacgaaca
     7561 atgatttctc cttggaagat catcactggt tgtacagctt ctcgtgcgga ttgtttcaat
     7621 tcttctgtct ttttctcatt caatacgtca ttcactacga taccttgatc cactaagtag
     7681 cgaatcattt gttgttgtgc tggtgtgaca ttcaaatatt ggatttgctc ttctgcttct
     7741 tgtttaaaag cttccaattc actttcacga acttgtttgc tcatctgttc gtcaatgagc
     7801 ttcagactct catccctcac agtatggatc tcagtttcag tcatttcaaa tatcgtcgaa
     7861 taaaaattgt tcggtagttt ttgataaaac gcaacatttt ctgcattcac attttcaaaa
     7921 tttttcttca acgcggcgat ccgctcttct gtagtaggct ttgggatatt ttcatcttcc
     7981 tttgccttat caatcttttc gtcataagat ttgttgatgg catcattcgt tttatcgatc
     8041 aattcaaata gttgcttgat ccgattgttc tggtcatctg caagatcttt ttggtaagtg
     8101 tattctggag tgactgcttc tgctgccagt tttctttttt gttcggtttc ttccgtgttc
     8161 tcgatcgttt tgttcgcacg gatactttct tctgctactt ggccttctct gaaatcattg
     8221 gccttttgtt tgacactact aaatgtaacg ccgcagataa tgataaaaaa caagaacagc
     8281 aggaaaggca atgctgcctt tccaagtttt tcttgtagtt tctgcagtgt tttattcaac
     8341 atgtgcttat cctcctaagc aaagcttatt cttgatggag atctgccttt tcataagctt
     8401 tgatgatttc ggcaactaca gggtgccgaa caacgtcact tgcttcaaaa ttaacaaaat
     8461 cgattttctt gatttgcttt aatgttcgtt ctgcatgaac aagaccactt gttgtccctc
     8521 gaggcagatc aatctgactc gtatctccat tgacaatcat ttttgattga taaccaagac
     8581 gtgtcaggaa cattttcatc tgagcaactg tcgtgttctg tgcttcatcc aaaataacga
     8641 atgcatcatc caatgtccgc ccccgcatgt aagctaaagg agcaatttcg atcacgcctc
     8701 gttccatcaa tcggttcgta tgatctaatc caaaaatttg gtataaagca tcataaacag
     8761 gacgcagata aggatctact ttttctttta gatccccggg aagaaagcct agattttccc
     8821 ctgcttccac agcaggccgc gtcaaaatga ttttttgcac ttcacctttt ttcaaggcag
     8881 cgattgctaa tactactgca agaaaagttt ttcctgtccc ggctggtcct acgccaaata
     8941 cgatatcatg tttggctacc gattcgacat atttcttttg gccgctgttt tttacccgta
     9001 ttggttttcc attacggtct ttgacgatct cttcctcata catttcaaca aaataatcta
     9061 atgtgccttt ttgccccatt ttcagtgccg tcacgacatc tggtgtactt acatggattc
     9121 ctcgtttgat caattcctgc aatgtccgaa tgactgaggc agccaaggaa atctgggatt
     9181 gttcgccaat gatctgaatg atttctccac gtgtatggat ggttgtttct gttgtttctt
     9241 caatcagttt tatatgcttg tcgtgtgcac ctaaaagcat gctgacatca tcagcagccg
     9301 tcaatctaat ctctaaagaa ctagactctt ccgtcaaaaa tagacatccc ctttttttcg
     9361 attcattctt tcctatttta ccatactcag aagaaactat atagcaaata agaaaaggtt
     9421 ctaaaagagt tgcttaacca taagtaccaa gaaacgttaa aaaatagctg cttccattct
     9481 ctaaaggtct cgtttgaata aaaaaagctg atcatgtaag ctatgcataa gatagaggac
     9541 aagtattccg tctaacttag caagtattct ctgggtattt ttcttgtttc aaaaaaagaa
     9601 aacaaaatat ttgaaatatt cttgcatttc aaaaaaaaca ggtgtattat aacgtcaaca
     9661 aataaatata cagcaaagcg tcgaggaaaa cagtaggcta ttcagcgatt tattcccagt
     9721 gagtcatgta agtgagaaat gacaataaat cgaatagacg aacatcattt ccgagtcaat
     9781 gtcgaaaacg tagatcattg tggctgcctc cattatcagg ctagcggata actttatcga
     9841 aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa ggtggtaccg ttctagaatc
     9901 tagagccctt ttcccaagtg gaaaagggct tttttttata ttttaaggtg gtggttttca
     9961 tggaatctga tattagtggc atttctagtt tctgcaacgt ttattttgtt actcaaaatg
    10021 ataattattg gaggaaaaat ttatggaaga aacaaaaact tattcatgga agcaattatt
    10081 gatggtcagt tcacttatct tcgggatgtt cttcgggtca ggtaatctga tctttcctgt
    10141 ccacttaggt caaatggctg gatcaaactg gatcagcgct ggtgtaggat ttgccatctc
    10201 aggcgctctt ttcccattat tagctatctt agctgtagta gtgacaaaaa gtgatggtct
    10261 gtatgactta gctaaacctg tcggaaaatg gtatgctgcc ttgttcttga tccttgttca
    10321 tttaacgatc ggaccattct ttggtacacc gcggacagca gcaactgctt ttgaaatggc
    10381 tgctaaaccc tttttgcctg aaaaatatga ttcattaggt atgtttttat tctctgctct
    10441 tttcttttta cttgcttatt tccttactgt acatccaaca cgtttggtca aatatgtcgg
    10501 aaagtattta aactccttgt ttcttgcatt attagctatc atttttgtcg tttctttatt
    10561 ccaacccatg gggaatctaa accaatctgc tgcagattct tatcagacga acgctttgac
    10621 aaatggcgtt ttagaaggat acaacacggt tgatgcagta gctttacttg ctttaagtgt
    10681 cacttttgtc catgcagttc gtgatcttgg ttttaaagat aaaaaagtca cagaattaac
    10741 agcaaaagct ggtagcctga gcgttgcgtt agaagtcatg atttattttg gtctggtcct
    10801 tctcggtgcg atgagtttga ataagatgcc tttatcagaa aatggcggaa ctgcactttc
    10861 tcagatcact aatttttatt taggtaactt cggtgctagc ttcttagggg taatggtcac
    10921 attaggtgtc ttcactaccg ctatgggact cgttgtttct tttgcacaag atttccataa
    10981 actgttccct aaagtcagtt atatgacatg gctgcggtta acgacttttg tctcttttgt
    11041 cgttgcgaat gccggattgg ataatatcat ccaatggtct ttaccagtat tgatgctttt
    11101 gtatccattg tcacttgcgt taatcttgct ttccttaaca gcaaaattct tccaaaagag
    11161 tccatttgtc tatcaagtaa cgatgttctt ttctgccgtt ccagccgtat tggatatgtt
    11221 ggcaaattcg cctgcattag tgagtcagca aactgtcgta gcaagcttac taggatttta
    11281 tcatcatcac gttccttttg ctgcattagg acttggctgg atggtcccaa cgttacttgg
    11341 ttacgctggc agcttactct tttattacgc ctatcgccta tcagggtata aacaagaagc
    11401 aaatgaacta ccagaagaat aaccaaatca aacacaattt aacataagag at
//
LOCUS       DAGCWU010000085        11451 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.085, whole genome shotgun sequence.
ACCESSION   DAGCWU010000085 DAGCWU010000000
VERSION     DAGCWU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.085"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            143..1783
                     /locus_tag="MBZ71_001729"
     CDS             143..1783
                     /locus_tag="MBZ71_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBT4429685.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1937..3259
                     /locus_tag="MBZ71_001730"
     CDS             1937..3259
                     /locus_tag="MBZ71_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBT4429686.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3321..3752)
                     /locus_tag="MBZ71_001731"
     CDS             complement(3321..3752)
                     /locus_tag="MBZ71_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4429687.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3816..4652)
                     /locus_tag="MBZ71_001732"
     CDS             complement(3816..4652)
                     /locus_tag="MBZ71_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBT4429688.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4842..5144)
                     /locus_tag="MBZ71_001733"
     CDS             complement(4842..5144)
                     /locus_tag="MBZ71_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBT4429689.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5170..5601)
                     /gene="ruvX"
                     /locus_tag="MBZ71_001734"
     CDS             complement(5170..5601)
                     /gene="ruvX"
                     /locus_tag="MBZ71_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBT4429690.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5598..5864)
                     /locus_tag="MBZ71_001735"
     CDS             complement(5598..5864)
                     /locus_tag="MBZ71_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBT4429691.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6118..6843)
                     /locus_tag="MBZ71_001736"
     CDS             complement(6118..6843)
                     /locus_tag="MBZ71_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBT4429692.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7209..7279)
                     /locus_tag="MBZ71_001737"
     tRNA            complement(7209..7279)
                     /locus_tag="MBZ71_001737"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7245..7247),aa:Gly,seq:ccc)
     gene            7396..8148
                     /locus_tag="MBZ71_001738"
     CDS             7396..8148
                     /locus_tag="MBZ71_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBT4429693.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8164..8643
                     /locus_tag="MBZ71_001739"
     CDS             8164..8643
                     /locus_tag="MBZ71_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBT4429694.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8894..10474)
                     /locus_tag="MBZ71_001740"
     CDS             complement(8894..10474)
                     /locus_tag="MBZ71_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBT4429695.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10467..11210)
                     /locus_tag="MBZ71_001741"
     CDS             complement(10467..11210)
                     /locus_tag="MBZ71_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429696.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KRLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
BASE COUNT         3484 a         2288 c         2042 g         3637 t
ORIGIN      
        1 ataaaaagaa caaataccgc tattttgatt tcttttgact ctattactca tataaataaa
       61 atttattata cctgctcttt ttgactatat cctcatctga ctcctatact tcgtttgtat
      121 taaaacttag gaggaatcat tcatgcttac agggctaaac ttattaaata atccttttct
      181 aaataaagga acagctttta caaaagaaga acgggcaaaa tacggaatca ctggtatgct
      241 tccaagtact gtccaaacat tggaacaaca atctgtccaa gcttacggtc aatacttaag
      301 caaacagacc gatttggaaa aacgtatctt cttaatgaat ctattcaata cgaaccggac
      361 attgttttat aaattgatgg gcgaacattt agttgaattc atgcctgtcg tttatgatcc
      421 tgtcgttgcg gattcaatcg aacaatacaa cgaaattttc ttgaaaccac aagatgctgt
      481 atttctttca atcgacgaac cagaaaatat caaagcaagt ttgaaaaacg cagcagatgg
      541 acgcgacatt cgtttgatcg ttgtgacaga tgcagaagga atcttaggta tgggtgactg
      601 gggtgtgaac ggtgttgaca tcgctatcgg taaattgatg gtctacactg ccgcggctgg
      661 tatcaatcct gcacaagtct tgcctgtatc aatcgacgct ggtactaata acgaagaatt
      721 attgaataat ccattatact tagggaatcg tcatgctcgt gtagaaggcg aaacttacta
      781 cgaattcatt gatcaattcg ttcaatcagc tactgaatta ttcccagaat tgttattaca
      841 ttgggaagat tttggacgag gaaatgctgc aacgatctta gaaaaatacg aagataaaat
      901 cactactttc aatgatgata tccaaggcac aggaatcgtt gtactagctg gtgtattagg
      961 tggattgaat atatcaggtg agcaattgaa agatcaaacg atcttgacat ttggtgctgg
     1021 tactgctggt gtaggtatcg caaacatcct tttagatgaa atgatccgtc aaggtgtacc
     1081 tgaagaagaa gcacgccaac atttctatca agtagataaa cagggtttat tgtttgaaga
     1141 tacaaaagga ttgactcctg gtcaaatccc attcgctcgt aaacgctcag aattcactaa
     1201 tgctgatgaa ttgacaaact tagaagcagt tgtaaaagca atccatccaa cgatcatgat
     1261 cggtacttct acccaacctg gagcattcac tgaagaaatc gtgaaagaaa tggcggctca
     1321 taccccacgt ccaatcatca tgcctttgtc taatccaaca aaattggcag aagcaaaagc
     1381 aaaagatttg attgaatgga cggacggaaa agctttagtt ggaacaggta tccctgctgc
     1441 tgatgtggaa tataacggtg taacttacca aatcggtcaa gcaaataatg cgttgatgta
     1501 cccaggttta gggcttggtt tgattgcttc aacagctaca cgagtaaatg ctgaaatcat
     1561 ttctcaagct agtcgagcat taggcggaat cgttgatgta acaaaaccag gagcagctat
     1621 cttgcctcca gtagcaaaaa tcactgaatt ctctcaaacg attgctgaaa ctgtcgcaaa
     1681 atcagttgtt gcacaaaaat tgaatcgtga agaaatcacg gatatcaaag aagctgttga
     1741 atctgctaaa tgggttcctg aatataaatc tttagaagat taagattgtt atagtaaaag
     1801 aacatacaga aactttgtat gacgtctaaa aagaaaaaag cgactgcggc aaaacagcag
     1861 ccagccaact actgctcttt tgccgcttct ctttggcttt tttcatacga tacagaataa
     1921 gaaaggatct gtaaaaatgg cacaaaaaac aacaaccgca actgcagttg aggaaaagaa
     1981 aaaagaaggg ttttggtcaa caaaaattac cggcgtaagt ttgccccttt atttgatcat
     2041 ggtccttgtc ctgatcattg caatggcttt aggaaaatta ccgacgaata tgattggccc
     2101 aatcgctgtt ttggtcattt tcggtaattt atttcatttt atcggaacaa aaatccctat
     2161 agtcaaaagt tatttaggtg gtggctccgt ttttgcgatc tttgcctctg ccgcgattgc
     2221 tacatttggg atcttacctg agtatgtagt aaaatccaca gaaaactttg tcagcaatat
     2281 gggcttttta gacttttata ttgctgcctt gattactggt agcatccttg gtatgaaccg
     2341 taatctattg atgaaagctt ctgttcgttt tattcccgtt gcacttattt caatggtcgt
     2401 ctcctttttt gccgtaggac tcgtaggtat gcttatcgga aatggttttg caaattccgt
     2461 tttatatatc tcattgcccg caatggctgg tggtgttgga gccggagctg tacctttatc
     2521 aacgatttat gcaaatgttt taggtacgga cgcttcttct atcatttccc gcctgattcc
     2581 tgcgacagca atgacaaatg tcctagctat cattggtgca gcgttagttg ctcgtgcggg
     2641 acaatcaatg ccaaaattca acggtaatgg taaattaatg gaaaaaggcg accttggcga
     2701 tggaaaacct agaaatgtaa aaccttccat tccacaatta ggtgtcggtt tgatggtatc
     2761 attgactttc ttcatcttag gacaaatctg taactatttt gttcctaaag tccatgctta
     2821 cgcattcatg atcattatcg ttgttatctg taaaatcgca aacctattac cagaatatta
     2881 cgaagatgca gctatcatgt tcaacaactt gattgtcaaa aacttgacag cagctgttct
     2941 tgccggtatc ggtattgcgt tattgaatct aaatgtattg gcttctgctt tgacatggca
     3001 atttgttgtc ttatgtttga caagtgttat cgcgatttca gtagtttcag gatttgtcgg
     3061 tcgtttgttt ggtttatatc caatcgaatc aactattact gctggcttat gcaacaactc
     3121 gatgggtggt acaggaaatg tagctgtctt atctgcagct aatcggatgg aacttattgc
     3181 ttttgctcaa atgggcaatc ggcttggtgg tgccatcgtt ttgatcatct caggtttctt
     3241 gatgcaatta ctttcttagt caaaaaaaat acctgatcat caagtatcgg cttgatgacc
     3301 aggtattttt tgataaaaat ttacaggatt ttcaaaaatc ttttttgcat tttatcggaa
     3361 tgataaccga tgcgttcata aaatcggtgt gcttctactc tgtattctgc agaattaagt
     3421 cttattgctg atattcctcg tcttttcgct tcttctacgc cagtcatcaa ggcctttccg
     3481 attcctcttt tttcagcatg tttagacaca gcaagtgcca taatattgaa catcggatcg
     3541 ctgaaaatcg attcatagga ctccgcttgt acatagccag ctgctttttt agaagtagga
     3601 tcttcataga ccaagaaaaa atttttttct ggctccttca acagtttctt catttgtctc
     3661 accgtaacgg ttaatggtac atcatatcct aattgttccg cattgatttc tcgtagtgct
     3721 tctgcatctc ctaaacgcat ttcccgcaac atccaaatca ccagccaatt tttatttttt
     3781 taaatgaatt tttttatcac agtatactcc ttttttcact taaaaaaaga cttttcagtg
     3841 attttgactt gcttgaatcc agatccttct aatgtttgca agatgacgaa agcattgtaa
     3901 agattgatca aaagaaccac gatgacaatc gcttgaaaaa taaaggtcac tttttttgga
     3961 gacaaaaccc tgctaaatct tgctccccac aaacctccaa taacagcagc ggggataaca
     4021 taaaacaaca tagataaatc aaagcgcata aagctacttg ttaaagcaat cgttaccaat
     4081 tttgccagtt gagaaaagaa aatcgtagat agagaatata gtgttgcttc ttttattggc
     4141 aatgcaaaca tcagcatcaa caaagaaaca ttgactggtc caccaccaat acctaaaaaa
     4201 ctagctaaaa agcccagaac ccccccacat ataaggtacc aagtccatga cgtcaaatga
     4261 aattttggct gatgatgctt ggtgtagaag aacgcaaaaa ccaaggtcaa taccgtcaaa
     4321 aaaatttgga tcagctgcac atgcttctcg ttttcaaata actgtaaaaa aacttcgaat
     4381 acaatgttgc cgactactcc tccaacaaca gctccactag atacccaacc gacaatctgc
     4441 caattcagtg atttcccgct tgatacttgt cgcacagtcg aaacaatgga catcgtgaat
     4501 accgcaactg ttgagtaaaa agaaatccct gcaacagaat gggcgccgat aagatcaaga
     4561 atcggtttga tcaacacgcc tccacccatc ccggaaatag cgccaactgt gttcgccaga
     4621 acgatcacca caaaataaat cagaccaatc atcttgtatt tcctttctct tgtttatatc
     4681 ttttagcgat tgttactctt ttcttttatt atacagtaaa agaaacaaac gaggcttcga
     4741 aagcttttct actttttaag gaaatttagt gctaaacgaa aaaaccgggc tctacttttc
     4801 agagagtcca gcttttctta caccttatcg attcttttgt tctattcttc ttccgccatg
     4861 aatgtattga atacttcttc gatcatatcc cattctgcgt ctgtttcgat ttgttctaac
     4921 tcgccttctg ttccatcttc gttttcgatg tatgcataag cttgtaattc tacatcttca
     4981 tcttctgaaa caccagctgg atataaaaga acatagttac gtccaaattc ttcttgtccg
     5041 tcaactgtta gcaagatttc gtataatgtt tcatttcctt ggtcatccac caatgtgatg
     5101 tgttcgtgtc cttcgtgttc gtgggaatgg ttatgttctg tcataagatt acctcttttc
     5161 tttttattat taaaataatt gcttacctga gccgtctaaa taattttgta aaatcatcac
     5221 agctgctaat ttatcgatga ctttttttcg tttcgctctt gaagtatttg cctgctctac
     5281 tagcatacgt tctgcctgaa cagtggttag tcgttcatct tgataatcaa ccggcaagga
     5341 gaatagttct ttgatcttct ctccatatga catggaagct tctgcccttg gtccgatgga
     5401 attgttcata ttcttaggta atccaactac aaaacggtct acttcgtact gcgccactaa
     5461 ttccttcaac cgatcaaaac caaactcttc ttctgcctca ttgattcgaa tgatttctac
     5521 tccttgagct gtccagccaa agggatcgct cacagcaacg cctactgtct tagaaccgac
     5581 atccaagccc attactctca taaattcact ccatgatttg agaggtagtc tttgacaatt
     5641 acttccatga tttcatcgcg ttcgtgacgg cgaatcaaat ttcgtgcatc acgataacga
     5701 ggaatgtagg ccgggtctcc agaaagcaag tagccgacaa tctgattgat tggattgtag
     5761 cctttttctt ccaaagcccg ataaacaatc gctaatgttt cgctcacttc ttttttgcga
     5821 ctatcgtcaa aaccaaaacg tacggtttca tctgtaaaac ccatgattct acacctcttt
     5881 cccgcaaggt aattccagct taccttctac acccttattt tactcgaaga aagggaaaac
     5941 tacaaacaaa tattccaaac ttctgaataa attaagaaac acgatttgtc tgatttttcc
     6001 ttttttctgt ggctttttca aaagccctct ttccataaag tatacgctat atttctttga
     6061 aagtatgttt ttacacaatt ttttcataaa gttcataaaa aattcgaatt gctgctacta
     6121 atttttcgtt ttcgttgctt gccctgtctc ataattgatg gtgtagccat cttgaacatt
     6181 gtagataaag acattgaatt caattttttt gtcttcaatg ctttctgctt ccaactgcac
     6241 acctcttgct accagttcat ttccctcgaa atgaggagtg acacgatagc gtacatgatg
     6301 atttgtttcc ttgatatatg ccgcaatatc attttcgtgg gcaagcatgt acggagcatt
     6361 caacgaacgt gtccctgtca ttaagttttt aatgttatta ttttgaccag tcaactggaa
     6421 accaatcaaa tggctgcggt tgtataacca ttgtccgtca cttaatttct tttgtttcca
     6481 acctgtcgga tcaatataca aaggttctct cttttcagta ggaaacaagt cttttcccaa
     6541 catcgcattg gcttgtccta ctctgttcaa tttgtctata tttgaatagc tttcccatgt
     6601 ccctttggaa agactaagat cctcttttgt aaactccggc tgattcccat tgatttctat
     6661 ttcttgatgt tttccatcat attctggcac atccgctact tgagaagctt gcggtgtgga
     6721 atcaaaaatg gttgtaattt gttggaagat atctgaatct ttttgtgtgg tggaaatacc
     6781 gagtgcccct gcaatcacag cagcgacgat ccccacgatc gccttggttg tttttttgct
     6841 catttcttta ctcctttact aacagttccc taaatcttaa catatgttcc cctataaata
     6901 aatactggaa agaaatagaa acagtaaaaa aataaaacca gtttttagac ggcttaagaa
     6961 tacaaaaaaa gagacggatt catcgtctct actcaatcac aaaacactat ttacaaggta
     7021 tttattatca ttcaaaaaat ttttcttaat actcaaaact aaacggcttc tgtacgacct
     7081 cttggtaaac agtgttcaaa agcttgcatc tgcggtaata agtccaacca agtcaaaaac
     7141 ataagaagct tttttgcttg actattctta ttacttgatg cagggcagct ttttcacagc
     7201 ctcttttaag cgggtgacgg gaatcgaacc cgcgacatca gcttgggaag ctgggatttt
     7261 accactaaac tacacccgcg atacaaaaac agtatacgcc tctttcactc cttcctcaag
     7321 tttttttatt ctttacgctt ccgatatgtt ttgtagtaaa ataaaactaa tcaatcaatt
     7381 attggagaag atgctatgac gggaaaagaa ttagatctgt tgctggacac ttgtttactc
     7441 gctgggaaaa tcatgatgga aagcaatgcg gagatgtatc gggtagaaga tacgatgagt
     7501 cgaattgctt tagcttctgg aaatttccgt ttggtcagct atgtcactca aaccggtctc
     7561 tttattggtc ttgatcgtac ctcgaccatc cgaatggaac aaattaccaa ccgttcgatt
     7621 aatttagaaa aagtagtaaa agtaaatgat ctttcacgta aatatgttgc ttcagagcta
     7681 acattagaag aactttactg cgaactgcaa gcaatcgacc gagaccgttc tttctttcct
     7741 gtctggttgc aaatcgttag cgcggcagtg atcagtggaa caattatggt gttatttggc
     7801 ggaaagatga ccgatttacc tgtgacttta gtcattggag gattgggata cgcagcttat
     7861 ctatatagtt tgaaattttt tagaatcaaa tttttatcgg aatttttatc ttctttattg
     7921 attggcatag ccgctatcta cagcgtacgt tttggcttag gaagcaatca ggatctaatc
     7981 attatcggat gtgtgatgcc tttagttcct ggagttcaaa ttacgaacgc tatccgtgat
     8041 ttgctagctg gacattatgt ctcaggagtt tctcgcggaa cagaagcgat gatgactgct
     8101 acgatgatcg gttttgcgat tgcttttatt ttccagcttt tttattagga aaggaacaca
     8161 agtatgggga cgatatttat tcagttttct tttagttttt tagcgactgc ggcatttgcc
     8221 gttattacaa atgtacctag gcgttcattg atttgttgtg gtctttctgg tgcttttggc
     8281 tggatgatct actggatctg tgttcaaata ggcggatctg ctgcttttgg ttcattgctt
     8341 ggttctttag gagtagcatt catcagcgat cttttctcta aacgtttgaa aatgcccgta
     8401 acgattttca atattcccgg gatggttcct ctagtccctg gtggtttagc ttatcaagcg
     8461 gttcgaaatc tcgttactga atcctatcga gaagcggctc attatgcggt ccaagcaatt
     8521 atgattgctg gagcaatcgc tttaggtctt gtattatcag aagtctttaa ccataacatc
     8581 cgtaatttca aagtaaagcg tgacgcaatc atgatgaaac gaaaaaaacg gctttttaaa
     8641 taaacgaagt gtaacaaaga aaaggttgtg aaagagacgt tcagccttga gcgatgacaa
     8701 aatatctcac aaacattgcg acaagtaacc gcaggagcaa taagaagtaa ttatgagtat
     8761 taaaaaaagt tttgtaaaaa tagcttctca catttttaca taaaattgac ttaatactga
     8821 gaaactgact tttgaacacc ctctatctgt aaaaaagggg ctgcgacatg atttttgtca
     8881 cggcccctct tctttattca aattttttcc aaaacgttcg ttgataataa acgatccata
     8941 gagcggaaag tattagaact aaaccgatca caggaatatt cactaaccaa aatggataaa
     9001 acaaagccaa cattccgtat aagacgataa gaataatact gatgatcaat gaacccatca
     9061 tagttgcgac taaacccaaa cttcctgaac ctctgttaaa taattggtta atattggtcc
     9121 agttcagcag cagcaaccga taatctctag cgaaataacg tagtgacaat aagtagcagc
     9181 cgaataaaga accaaatagc aagctaggga taaaaaacaa aggcagctga aaagacagtc
     9241 cgcttaataa tgcgatccca ccagttaaaa aactctgcaa tatccatccg aatctgaact
     9301 tttcttttaa atattggttc atagagattg gcaaagaacg gataaacaga aaattttctt
     9361 gatccaaaga aataagattg ctgataaaag aagtctgatt aactgttaag aaagccaatg
     9421 cgatccctac caggaaaaca acaccgatta acttattatc taaatcagtt aattttattt
     9481 cgcctcctag ggcaaaagca agaataaaaa tgattggagt taacaaggaa gaagaaagaa
     9541 cttgcataat caaattaggt tcttttacta attgagcatt gtaactaaat aaaagctgat
     9601 gcacattttg gttcttttta tgggttcttt ttattttgaa ctcactgttt gaatcagtca
     9661 gttgctctgt aagctttggt actatcagct gttttacagc aaggaaaaat cccagagccg
     9721 tgatagctag tattccccaa cccaataaac cttgttgaga aagtggctga ctggcagcat
     9781 catagaatgg caataatatc gaaatcggat gccgatctgt taactcattt tcaatagtcc
     9841 ccgtttgatg attcatccac atgattccag caaatgcgat cagcatcgag ctgccaagca
     9901 agatactaga aactgttttt ctatgttttt taaacactgc tgttcgagcc aaaccaaaaa
     9961 caatcaaact agccagacaa aaaagaataa tcaaaaatag agaaaacaaa aggattgaga
    10021 taatgatagt aagcaaaatc cctagatcag ctcgccatcc ggttaaaata aacatgacta
    10081 gaagcggaaa aacaaaagga atgatggtca aagcaactac caaaatcttt gctgaaaaaa
    10141 cctgttcttg tccaaaaggc agaggcaaaa atacttgcag gtcatttcct tcaaaaaaga
    10201 tattataaat gactgatata ctttgagtta aacctaacat accaaaaagt gccacgtaat
    10261 aagtaaagaa acctggcaat ttaacaaagt tcatagcaaa catagcaaat ccgtaaatca
    10321 gtaaaaaaat cagccctgaa aaaagatttt gagacaaaag atagatagtt aatcgctttc
    10381 ccttttttcc tttctctctt gctttattcg tgagctgtgg attaacatat aaaagattaa
    10441 ctcgaattaa ttctaataat tgtcgcttat acatacggtc catctcctgt cgcttgcctt
    10501 ccagccattt tcaaataaat cttttctagc gtttcatcag gtgagccatt caataattca
    10561 ctaactgccc cctgataaat caattcacct ttcttcaaaa tagccagttt atcgcataac
    10621 tgttgtgcgg tatccaatac atgtgtagaa aaaatgactg tttttccttt tgcggcatga
    10681 gccttcatca tttctttcaa atcaaatgct gcttgaggat caagtccttg caaaggttca
    10741 tccaataccc aaatatctgg atctggcagc aacgtgccga tcaaaatcgt tttttgacgc
    10801 attccatgtg aaaaacttgc gattgtttca ttttgatgac tatacatatc aaaaagcgct
    10861 gtcaattctg ctaaacgctt ttcttttttt tgtgtgtcta attcataggc agctgaaatc
    10921 aagtcccaat attctccagc cgtcaattgt aaaaaaatat ctggggaatc aggcacataa
    10981 ccgatttttt gtttgatact caatcgattt tccgtcaatt tcattccatc tactaaaatc
    11041 gttcccgatg atggttcaat aatactaact aatcttttaa tcgtagttga ttttccggca
    11101 ccattatgtc ctaaaaatcc aaaaatttcc ccttgtctta tcgagagaga caagtctttt
    11161 aatgcttgtt ttttcccgta acttttactt acattgatga attcaatcat ttctgtactc
    11221 cttttgtttg ttattacttt ttattgtacc cttcgttatt ggtggagtaa atgtcaaaaa
    11281 gagtgcataa caaaaaagga tagaaacgac taggctgcta cttgggttgt tctcttctac
    11341 aggaaaacac tcttctaaca ggtaagatat gagtctttgt tttctttaaa atgattcatt
    11401 ttctatcttt tcttgcataa aattctttag tttatcaata cttctctttc c
//
LOCUS       DAGCWU010000086        11442 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.086, whole genome shotgun sequence.
ACCESSION   DAGCWU010000086 DAGCWU010000000
VERSION     DAGCWU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.086"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            236..991
                     /locus_tag="MBZ71_001742"
     CDS             236..991
                     /locus_tag="MBZ71_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HBT4429697.1"
                     /translation="MKELIKVTTNEENEQLVNGRELHEFLEVATEYKKWFSRMAEYGF
                     VENIDFVRVTQKCPTPGGIQNITDHAMKLDMAKEISMIQRTEKGKQARQYFIEVEKEY
                     KQQLLDTSKLSPELQMFQGIFNAVAKQELETKRLATQMENITEIVALNTTDWRRECRK
                     LVNKMAETQGGYGAYQEIQTAIYEEVDRRAGSSLKTRLTNLRNRMAGEGVPKSKRDKT
                     NKLDVIESDKRLKEIYLSVVKDFAIKYGVWKEK"
     gene            1012..1278
                     /locus_tag="MBZ71_001743"
     CDS             1012..1278
                     /locus_tag="MBZ71_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429698.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALNQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLVDHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            1292..1564
                     /locus_tag="MBZ71_001744"
     CDS             1292..1564
                     /locus_tag="MBZ71_001744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429699.1"
                     /translation="MTEITITDGHMSHTVKTKEEAQRIIDRYFPEGESTMQIRKPIVE
                     RSSSKMLLASIAEDKEPVYKVRQLQALCYISLVLNVILLSVVFAVL"
     gene            1583..2251
                     /locus_tag="MBZ71_001745"
     CDS             1583..2251
                     /locus_tag="MBZ71_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429700.1"
                     /translation="MSYNKWTREEEQLLIQNVRYDHRGFVCNCNELAELLGIDKKRIW
                     PKIHQMCKKDLIDEVYWDDPIEPPNKRYSQQEDKKIISMYQSGCPVTIIAQELNRTEN
                     AIRCRLNIMKKNGQLESNRKRRYTKREVELLISEIKFDKNGYVLNIDYLTRLLHRPKH
                     ELFRKICLLRKQGEITIKPDRTKASQNWYDVMKKQIDNHHKLIAARYSYQKKTRSPAS
                     KIVS"
     gene            2283..2612
                     /locus_tag="MBZ71_001746"
     CDS             2283..2612
                     /locus_tag="MBZ71_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429701.1"
                     /translation="MNEKIQNLIKELVAECANDDLGMSVSVVDEKGEVVLGQAGNDSL
                     VAWSVYRQYEKTKRDLQNNNCNCETHSTLKQLFGIEYEDPEFEEWYQDFLRFAEKMDR
                     KKGGIKF"
     gene            2612..2836
                     /locus_tag="MBZ71_001747"
     CDS             2612..2836
                     /locus_tag="MBZ71_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429702.1"
                     /translation="MISVKGLGDEMFEAMMHKAQQDVQDKILTAAKYGQTSCTVRSKG
                     LTPSFLAALESEGISNIQREEGSVKLFWEF"
     gene            2847..3197
                     /locus_tag="MBZ71_001748"
     CDS             2847..3197
                     /locus_tag="MBZ71_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429703.1"
                     /translation="MSVKDVAKKQILKMEHIIDQINEAEDLLISLKSPALNNLNELTT
                     DISIGVPTQFLGRGFFQEEQSRWHKVQLERDLGITEIQEEIRNLVSKSVQKRIEALES
                     ELRAMIYYRGDHDE"
     gene            3190..3384
                     /locus_tag="MBZ71_001749"
     CDS             3190..3384
                     /locus_tag="MBZ71_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429704.1"
                     /translation="MNDFDSLGARQQPPNEASPVGVDWQGNPLYPGDTCYLTEEGYVP
                     VDDILEYAQQHYPKIELGGI"
     gene            3387..3986
                     /locus_tag="MBZ71_001750"
     CDS             3387..3986
                     /locus_tag="MBZ71_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429705.1"
                     /translation="MDKSIRTDSDIVNHIKRLNLDGFSAGRSLVGNPATIADEKTIGL
                     FKEIKKVVKENGASYDQTRKALIMVDESFYQNLLKKENPFKEKQAEPINEPKKEDINV
                     TREDLESAELTIDPDLPGKLEKELRELSKEFFNKILASKKELLPSMKEEELVFLHKKL
                     NEFRRETPSDGFDANFHIGQILTDVILIVFAGDDLKYAD"
     gene            complement(3970..4320)
                     /locus_tag="MBZ71_001751"
     CDS             complement(3970..4320)
                     /locus_tag="MBZ71_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429706.1"
                     /translation="MYKLKKDNYNELSGICRSVMQNIKDLEEGSKMDLFDPRISDEEY
                     YLYSSVLKSYNSLGNQLLSNWETLKFYDIDAFFTKLSTYVEYLDIFIKEPTIENQLRL
                     TSIYDSLDKLNQRI"
     gene            4374..5396
                     /locus_tag="MBZ71_001752"
     CDS             4374..5396
                     /locus_tag="MBZ71_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBT4429707.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLANGKETTTVMTKKQIDQSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQDEVTRVADVPGHSEIEQAHLIENEDTDPIQEELLDIPDFGREEGVDDVSEFE
                     DDEYPF"
     gene            5359..6174
                     /locus_tag="MBZ71_001753"
     CDS             5359..6174
                     /locus_tag="MBZ71_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HBT4429708.1"
                     /translation="MSQNLKTMSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWSPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTAWKGKIDCLNVEEEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLYQQYGKSFMPIIAAVSKQTPSEARLITI
                     DQDNMDYELVMLKEKIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            6190..6969
                     /locus_tag="MBZ71_001754"
     CDS             6190..6969
                     /locus_tag="MBZ71_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4429709.1"
                     /translation="MAEGWVKLHRSITENWIWDNPQYLKWWLDLILMANHKEKKILFN
                     GSFKKVDVGQRITSEQKLAERWGVSRNTVRKFLSLLVEDDMISIEKSRKNGTTYKVNN
                     YAIYQGFSEEKKQQTEQRAEHQKDNELNINKNEKNEKNDKNNNNLRNSRKTREYADDD
                     PNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSNDFW
                     SGVILSPTSLRKHFDKMAIQKNKRTQQNISNDELPETGEDW"
     gene            6969..7817
                     /locus_tag="MBZ71_001755"
     CDS             6969..7817
                     /locus_tag="MBZ71_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4429710.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVSGSTIHAVITGRTGAGKTHLGAAVIKEVMMASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKTDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            7814..8056
                     /locus_tag="MBZ71_001756"
     CDS             7814..8056
                     /locus_tag="MBZ71_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429711.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            8199..8654
                     /locus_tag="MBZ71_001757"
     CDS             8199..8654
                     /locus_tag="MBZ71_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBT4429712.1"
                     /translation="MATRSKYGNKKHEVDGITFDSKAEARYYMKLKRNGMSFMPLSET
                     YCAMQENVLLQEGYLCNDRKIAPIYYRADFVIYENGQVKKVIDVKGYQDAISMLKMKM
                     FAHRYGFPVTFAKFDSKINKFIEMDCFESARQQRKRQTERRKKKLMEGK"
     gene            8656..9231
                     /locus_tag="MBZ71_001758"
     CDS             8656..9231
                     /locus_tag="MBZ71_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429713.1"
                     /translation="MTKQVNFRPELKKVTSKSNGNTEVLLVVSNGSLRGSTENLTEFL
                     GSTVTVVIQPETIEYTVPVNKQTKKPNVEYVVNADGTIDMLKEEQTSLDVGDGVEEVE
                     NVKILVSKETIDEFIKTATTLQLPENVTVNIRDVLIRLAEGDSMSEIAADHELSEIAL
                     IDQIELARHYFAPYADSWSKHKDDIIFPEEQ"
     gene            9228..9416
                     /locus_tag="MBZ71_001759"
     CDS             9228..9416
                     /locus_tag="MBZ71_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429714.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMDQ
                     KILDVGLLLLHLAEKNLI"
     gene            9424..9630
                     /locus_tag="MBZ71_001760"
     CDS             9424..9630
                     /locus_tag="MBZ71_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429715.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEASQWLLKTYTNNKKLRKKCPDL
                     FLKDDQIMPEPMILTSKEGGKCES"
     gene            9794..10192
                     /locus_tag="MBZ71_001761"
     CDS             9794..10192
                     /locus_tag="MBZ71_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBT4429716.1"
                     /translation="MIPRFRAYSKEENEMYYPHNDKNVDWTIDDETGFIAPLVNLGGG
                     MWGMIDKYELMQSTGLKDKNGVDVYQGDIIRCTRGCHHEVIWLEEYGGIFFGGMPAWY
                     LSGLSNGYAWTGGEEVIGNIYENSELLEEK"
     gene            10192..10491
                     /locus_tag="MBZ71_001762"
     CDS             10192..10491
                     /locus_tag="MBZ71_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429717.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMKCDKWLAKSKENLAVVRLVDMEVINNEI"
     gene            10481..10639
                     /locus_tag="MBZ71_001763"
     CDS             10481..10639
                     /locus_tag="MBZ71_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429718.1"
                     /translation="MKFKILEEDTRYELEEKLNEFAKNNEIQHISLTAFMAGYTAYYA
                     AVVSYVSQ"
     gene            10737..11153
                     /locus_tag="MBZ71_001764"
     CDS             10737..11153
                     /locus_tag="MBZ71_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBT4429719.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKYGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         4278 a         1747 c         2448 g         2969 t
ORIGIN      
        1 agaagcagca aaactctttg atgttcacca tcaaacctta gcaaattggg aacaagatcc
       61 gaataaaatg aagcaaaaat atgtgcaatt aataccagaa atataccatt ttccaacagc
      121 aaatattttt tttggaagca aagacgagtt tatacgatat aaacttcata acgattcatt
      181 tttaataaaa taacttatgg aaaacatatt tattcatttt taaggagtgt aatccatgaa
      241 agaactaatc aaagtaacaa caaatgagga gaatgagcag ttagtaaacg gcagagaatt
      301 gcatgagttt ttggaggtgg caacagaata caaaaaatgg tttagtcgta tggcggaata
      361 tggttttgtt gaaaatatag atttcgtaag ggtgacccaa aaatgtccga cccctggagg
      421 aattcagaat attaccgacc atgcaatgaa attggacatg gcaaaagaaa tctcaatgat
      481 tcaacgaaca gaaaaaggaa aacaagcgcg tcaatatttt attgaagtag aaaaagaata
      541 caaacaacaa ttacttgata cttcaaagct aagtccagaa cttcaaatgt tccaaggaat
      601 atttaacgca gtagctaagc aagagttaga gacaaaacgt ctagcaacac aaatggagaa
      661 tattactgaa attgtggcat tgaatacaac tgactggcgt agagaatgtc gaaagctcgt
      721 aaataaaatg gcggaaactc aaggcggata cggtgcttat caagaaattc aaacagcgat
      781 atatgaagaa gttgatcgta gagctggctc ctctctcaaa actagattaa ccaatttacg
      841 taaccgtatg gctggcgaag gtgttccaaa atccaagcga gataagacaa ataagttaga
      901 tgtaatcgaa agcgataaaa ggttaaaaga aatttatcta tctgtagtca aggatttcgc
      961 tattaagtat ggagtttgga aagaaaaata aaattcgata ggaggcaagt catgaatatt
     1021 ctaagcgaag agtttctgac tcgtttgaga attgcaattg ttgaagttgt gaaggacgca
     1081 cttaatcagc tttcaaaaaa gaatctttca gaaacacgat atttgaaaaa aatcgaagct
     1141 agaaagtatg tgggaggtgt aaatgatcaa ggctttgaga agctagtaga tcatgggtta
     1201 aaagaaattc gtatagatgg ctttttaagg tatgacaaaa aagacatcga tgaactgatg
     1261 gctaaataca aaatctaaaa ggaggacata catgacagag atcaccatca cagacggaca
     1321 tatgtctcat accgtgaaaa caaaagaaga ggcacaacgt attattgatc gatattttcc
     1381 ggaaggggag tcaactatgc aaataagaaa accaatcgtt gagcgttcga gtagcaaaat
     1441 gctactggca tcaattgccg aagacaaaga acccgtgtac aaagttcgtc agttacaagc
     1501 actttgctat atcagcttag ttctaaacgt cattttattg tctgttgtct ttgccgtttt
     1561 ataaaaaaag gaggttagaa aattgtcgta caacaaatgg acaagagaag aggagcaact
     1621 tcttattcaa aatgtcagat acgaccatcg cggatttgtt tgtaattgta atgaattagc
     1681 tgagttactt gggatagata aaaagcgtat ttggcccaaa atccatcaaa tgtgtaaaaa
     1741 agatttgatc gatgaagttt attgggatga tccaatcgaa ccgcctaaca aacgttacag
     1801 tcagcaagaa gataaaaaaa taatttctat gtatcagtca ggttgtccag taacaataat
     1861 agcgcaggaa ttgaatagaa ctgagaacgc gattcgctgt cgtcttaata tcatgaagaa
     1921 aaatggacaa ttagaaagca atcgaaaacg caggtacaca aaaagagaag ttgagttatt
     1981 aatatcagaa attaaattcg ataaaaatgg ttacgtatta aatattgatt atttaactag
     2041 actactgcat agaccaaagc acgagctttt cagaaaaatt tgtctattaa ggaaacaagg
     2101 agaaataact ataaaacccg atcgtacaaa ggctagtcag aactggtatg acgtaatgaa
     2161 aaaacaaatt gataatcatc acaaactgat agctgctcga tatagctatc aaaaaaagac
     2221 acgatcgccg gcaagcaaaa tcgtgtcctg aaaaataata attacaagga gagtttatca
     2281 caatgaatga aaaaatccaa aatttaatta aagagttagt agctgaatgt gcaaatgatg
     2341 atctaggaat gtcagttagt gtagtcgatg aaaaagggga agtcgttctt ggacaagctg
     2401 gaaacgatag cttggttgcg tggagtgttt accgacagta tgagaaaaca aaacgtgatt
     2461 tgcagaataa taattgcaat tgtgaaactc atagtacttt aaaacaattg tttggtattg
     2521 aatatgaaga tccagagttt gaggaatggt atcaggactt tctacggttt gccgaaaaaa
     2581 tggatcgcaa gaaaggtggt atcaaattct gatgatctca gttaaagggc taggagatga
     2641 aatgttcgaa gcaatgatgc acaaagcaca acaagatgta caagacaaaa tcttaactgc
     2701 agcaaagtat ggacaaacaa gttgcactgt tcgttcaaaa ggtctgacac catcatttct
     2761 agcagcatta gaaagcgaag gtatttcaaa catccaacgt gaagaaggaa gtgtcaaatt
     2821 attttgggaa ttttaggagg caaatgatga gtgtaaagga tgtagctaaa aagcaaattt
     2881 taaaaatgga acatatcatt gatcaaatca acgaggcgga agatttactt atcagcttga
     2941 aatcaccagc actgaataat cttaatgagc ttacgactga tatttccatc ggtgtgccta
     3001 ctcaattttt agggagaggt tttttccaag aagaacaaag caggtggcat aaggtccaac
     3061 ttgaacgtga tcttggtatc acagagattc aagaagaaat tcgtaatctt gtttccaaat
     3121 cagttcaaaa aaggattgaa gcacttgaaa gcgagttgag agcaatgatt tattatcgcg
     3181 gtgatcacga tgaatgattt tgattcttta ggcgccagac agcaaccacc aaatgaagct
     3241 agtcctgtcg gagttgattg gcagggaaat ccgttgtatc caggtgatac ttgctatcta
     3301 acagaagaag gctatgtacc agtagacgac attctagagt atgcgcaaca acattatcca
     3361 aaaattgaat taggaggaat ttagggatgg ataaaagtat acgtactgat tcagatattg
     3421 tgaatcatat taaacggtta aacttagatg gctttagcgc tggccgttct cttgttggga
     3481 atccagctac tatcgctgat gaaaaaacaa taggtctatt taaggaaata aaaaaggttg
     3541 taaaagaaaa tggggcaagt tatgaccaaa cgagaaaggc cctcattatg gtagatgaaa
     3601 gtttttatca aaacttattg aaaaaagaga atccatttaa agaaaaacaa gctgaaccaa
     3661 ttaatgaacc taaaaaggaa gatatcaacg taacaaggga agatttagaa agtgccgagt
     3721 taacaattga tcctgattta cctggaaaac tagaaaaaga attgcgggaa ctaagcaaag
     3781 agtttttcaa taaaattctg gcatcaaaaa aagaacttct cccctcaatg aaagaagaag
     3841 aactggtttt tttacacaaa aaattaaatg aatttagaag agaaactcca tccgatggat
     3901 tcgatgcaaa ttttcatatc ggacaaattc tgacagatgt gattctgatt gtttttgcag
     3961 gtgatgatct taaatacgct gattaagctt atcgagactg tcgtaaattg acgttaatct
     4021 caactgattt tctatagttg gttctttgat gaatatatcc aaatattcga catacgtact
     4081 cagtttagtg aaaaaagcgt ctatatcata aaattttaag gtttcccaat tactgagcaa
     4141 ctgatttcct aaagagttat agcttttaag aacagatgag tataaataat attcttcatc
     4201 actaatacga ggatcgaata agtccatttt actaccttcc tctaaatctt taatgttctg
     4261 cattactgag cgacatattc cagaaagctc attatagttg tcttttttca atttatacat
     4321 ttttatcgcc tcactttcaa aataagtata gcaaaaataa aggagaataa aatatggcaa
     4381 atgacttaac acaaacaaca caacgttcat tagatgaaca ggtaatcagt aatcttggac
     4441 ggttacagga acaaggctta gaaatgccac caggatatag tccgcaaaac gcgttgaaga
     4501 gtgctttttt cgaactcact aacaatacag gtggaaattt attacagatg gctgcaaaca
     4561 atcaagaaat gaaaacctcc atttctaatg cacttttaga catggttatt caagggttat
     4621 ctccagcaaa aaaacaatgc tatttcatta aatacggaaa caaggttcag ctaatgcgtt
     4681 catattttgg aacaatggcg gtccttgatc gtgtaactgg aggggcagat attacgcctg
     4741 tagtcgttcg acaaggtgat gagtttgaag ttgcaatgga tggaccgaac atggttgtca
     4801 aaaagcatga aacaaaattc gagaatttag acaatgaaat cattgcagcc tatgtagtta
     4861 tcaagttagc gaatggtaaa gagaccacaa cagtcatgac gaaaaagcaa atcgatcaga
     4921 gttgggcgaa gtcaaaaatg aaaggttctg gaccacaaaa ggaatttcca gaagaaatgg
     4981 ctaaacggac agtaatcaat cgtgcagcta aaacattgat caacacaagc aacgacaacg
     5041 atttattagt tcaagctgca aaagatacgt tagaaaatga atttgacaat gatcggaaag
     5101 atgtaacgcc acaaacagaa aaagtggcta ctctcgaaca aaaattcttt tcaaacaaaa
     5161 agattactga accaatacaa aaagaaccag atccgattgt gattccagat gatatccaag
     5221 atgaagtaac tcgagttgct gacgtacctg gccattcaga aatagaacaa gcacatttaa
     5281 tcgaaaatga ggataccgat ccgattcaag aagaattatt ggatattccg gactttggac
     5341 gtgaggaagg tgtagacgat gtctcagaat ttgaagacga tgagtaccct ttctgatgaa
     5401 aattattact ccaatgaagc tgactggcag tacatgtcga catcacaata taagtccttt
     5461 ttaaaatgtg aagccgcagc cttagcaaag ttgaaaggcg attggtcacc gacgtcggat
     5521 cccaaagcct tactcgtcgg aaactacgta cattcttatt ttgaatcaaa agaagtacat
     5581 gaagcgttca aagaagaaaa taaatccaaa atgttttcta gtcggaagcc ctatggattg
     5641 ttaaaagatt tccagattgc cgaacaaatg attgaacgac ttaagcaaga agaagcattc
     5701 atgaatatct atcaaggtga gaaagaaacg attgtgactg gggaactttt tggaacggcg
     5761 tggaaaggca aaattgattg tctgaatgta gaggaagagt attttgttga catcaaaaca
     5821 accaaagaca tgcacgagcg gaaatggaat gaaaactacg gatcaagaga aacgttcatt
     5881 gtcaattttg gttacgtact tcaaatggca atttaccagg aactgcttta ccaacaatac
     5941 ggaaaaagtt ttatgccgat catcgctgca gtgtccaagc agacaccaag tgaagcaaga
     6001 ctgatcacga ttgaccagga taatatggat tatgagctgg ttatgttaaa agaaaaaatc
     6061 gaaagaattg tcagagtgaa gaacggagaa gagaagccga accattgcgg tttatgcgag
     6121 tactgtaggg gtaatcttcc aataactgga ttcactagca tggacgattt atagaacggt
     6181 ggtgattata tggctgaagg atgggtcaag cttcaccgtt ctataactga aaattggata
     6241 tgggacaatc cgcaatatct aaagtggtgg cttgatttaa ttctaatggc caaccacaaa
     6301 gaaaagaaaa ttctttttaa tggttcattt aaaaaagttg atgttgggca aagaattact
     6361 tcagaacaaa aattggctga acgttgggga gtaagtagaa atactgtcag aaaattttta
     6421 agtctacttg ttgaagatga catgataagc attgaaaaaa gcaggaaaaa tggaacaacg
     6481 tataaagtca ataactacgc catttatcaa gggttttcgg aagaaaaaaa acaacagact
     6541 gaacaacgag ctgaacatca aaaggacaac gaactgaaca taaacaagaa tgaaaagaat
     6601 gagaagaatg ataagaataa taataatctt cgcaactctc gaaaaacacg agagtatgca
     6661 gatgacgatc caaataaaaa attggccatt cttttattaa aactcattcg aaaaaatcaa
     6721 aacatcaaag aacctgattt ggataaatgg gcgaatacga ttcgtttaac aattgaatct
     6781 gacaaacgaa ctggtagaga agttcaagac atgattgttt gggccacaag taatgatttc
     6841 tggtcaggcg taatcttatc tccaacgagt ttgagaaaac atttcgataa aatggctatc
     6901 caaaaaaata aaagaacgca acaaaatatt tctaatgatg agttaccaga aacaggtgag
     6961 gattggtaat gaataaaaaa ctaagtgcaa tggctgcacc ttacggcgga ttaaaaacag
     7021 cagatcataa ttgcccgaaa tgtggtgatc cattgtatat ctggaaaaca aaaaataaag
     7081 atggtactga tcgatgcggt cctacatgta tcaataagat ttgtggttac cgagaaatgg
     7141 taactaaaaa tcaaaaagaa gctatccaaa aagcgaatga agcaatgaaa aaggacgcta
     7201 tcaatcgaat gcttaacagt tcaatgatta cagatgatgc catatggacc ttcgattttg
     7261 atggatacaa agtagttgat caggaaacag cacaaataaa agcgatggct caagaatggg
     7321 ctaaaaaaat cgtaagtggc agcacgattc acgcggttat tactggtaga acaggagccg
     7381 gaaaaaccca tctaggtgct gcagtgatca aagaagtaat gatggcatct aattataaaa
     7441 ttgcctgttc atttataagc tatcgagaat tattagagca attgaaattt gcaatgaatg
     7501 atccagaagc aagaaaagct gtgacaggtt cgttgatggc tgaaattaaa aagacggatt
     7561 ttgtagtgat tgatgattta ggtgcggagc taggaagaat ggaagaaaac aatcaggcaa
     7621 cgccttatga tgttgatgtt ctcacatcgc tcacagaagc tcgtctaaac aaagctacga
     7681 tattcacaac caatttatca tcgaagcaat taaaacacgc atacggcgag cgagtgttct
     7741 ctcgtgtaat gaatgggaca aaaggaaaca tagccgtatt caaaacaacg acagacaaaa
     7801 ggaggaatcc agtttgacct ttgtagtgaa aaaaatgtgc tacttagata gccgagggcg
     7861 aggagaagcc agtgtcgagt ttgctaaaca ccacacgact aaagaagagg ctgaccttgt
     7921 tgcaagtgtt tgtggcggcg aagtagtcga agttattaaa cccaaaagac gattttcaaa
     7981 acctaagaca aagccagtga aaaaagagtg ttgttgtccg aaaagtaacc aagcatggat
     8041 gagaggtgca aaatgacttg tttaaaatgc aacgatgaaa cagttatttg gtataagaca
     8101 tcgcttggat ggtcaacttg tgaaccttgt ccaatatgca atgaaaatgg acgacgttcg
     8161 aaagaacgac tcgaaagact aaagaaggag tatagcaaat ggcaacaaga agcaaatacg
     8221 gaaacaaaaa gcacgaagta gacggcatca cttttgattc taaagcagaa gctcgttatt
     8281 acatgaagtt aaaacgaaac ggtatgagtt ttatgccttt atctgaaacc tactgtgcca
     8341 tgcaagaaaa tgttctgctg caagaagggt atctatgcaa cgatcgtaag attgcaccga
     8401 tttattaccg agctgatttt gtgatttatg agaatggcca agtgaaaaaa gtgattgatg
     8461 tcaaaggtta tcaagatgcg atctctatgc tgaaaatgaa aatgtttgct catcgatacg
     8521 gctttccagt gacatttgct aagttcgatt caaaaatcaa taaattcatt gaaatggatt
     8581 gttttgaatc agcaagacag cagcggaaaa gacaaacgga acgaagaaaa aagaaattaa
     8641 tggagggaaa ataaaatgac aaaacaggtg aatttcagac cagaattaaa gaaagtaaca
     8701 tcaaaatcaa atgggaacac agaagtatta ctagttgtta gcaacggatc attgagaggt
     8761 agtactgaaa atttaactga atttcttggc tcaacagtga ctgtagtaat ccagccagaa
     8821 actatcgaat acacagtgcc agtaaataaa caaacgaaaa aaccgaatgt cgaatacgtt
     8881 gtaaatgctg atggcacaat cgacatgctt aaagaagagc agacttcact tgatgtaggt
     8941 gatggagtag aagaagttga aaatgtgaaa atcctagtat caaaggaaac gattgatgaa
     9001 tttattaaaa cagcaacgac attgcaacta ccagaaaacg taactgtaaa tattcgagac
     9061 gttcttatcc gtttagcaga aggcgatagt atgagtgaaa ttgctgcaga tcatgaatta
     9121 tcagagattg ctttaataga tcaaatcgaa ttagctagac attactttgc tccatatgca
     9181 gatagctggt ccaaacataa ggacgacatc atttttccag aggaacaatg agagcaactg
     9241 atccagtaat tatccttgag gaagctaaat ttatttggac tcacgaagag atagagcaag
     9301 cacgcttgct cttttctcaa ggagttaagc cgagcaaagt agctgaaata atggatcaaa
     9361 agattcttga tgtcggattg cttttgttac atctagcaga aaaaaatttg atttgaggtg
     9421 aaaatgatga ttcaacttgc aggcatacaa acagggaaaa tttatttttc cggagaaagc
     9481 aaaagtgaag ctagtcaatg gttgcttaaa acatatacga acaataagaa gctgagaaaa
     9541 aaatgtcctg atctattttt gaaggatgat cagattatgc cagaaccgat gattttgaca
     9601 agcaaggagg gcggcaaatg cgaatcttag agattgtttg ggaagtaatt aaatttattt
     9661 ccaatatgta ttttacgttt tggttagtgt gctgccttct atatggattc aaatggcaag
     9721 tagggaattt catgagcgtt aaattgccgg ggatattgag acggagtaaa agaatttaca
     9781 aggaggacag caaatgatac cgagatttcg agcgtattca aaagaagaaa atgaaatgta
     9841 ctatccgcat aatgataaaa atgtggattg gacaatagac gatgaaacag gctttattgc
     9901 tccacttgta aatttaggcg gcggcatgtg gggaatgata gacaaatacg aacttatgca
     9961 atcaacagga ctgaaagata agaatggtgt ggatgtctac caaggcgata tcatcagatg
    10021 tactagaggt tgtcatcatg aagtaatttg gttagaggaa tatggaggaa tattttttgg
    10081 aggaatgccg gcgtggtatc tttcaggatt aagtaatggc tatgcgtgga ctggtggaga
    10141 agaagtcatc ggaaatatat acgagaatag cgagttattg gaggaaaaat aatgaaacta
    10201 aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga catgccgaca
    10261 aagaatccta aaacacgtga gaaaccaaaa gtcaaagtcg gtgatatggt tcgctgtgaa
    10321 gcagaagggt tcatctatcc gtttcgtgga tatgtagagc atctctataa tcactcagca
    10381 atcattcgta ttgaaaacac gatgaaatgt gacaagtggt tagcgaaaag taaagagaat
    10441 ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta aaatccttga
    10501 agaggacaca cgctatgaat tagaagaaaa attaaatgaa tttgcaaaga ataatgaaat
    10561 tcagcatata tctttaacgg cttttatggc cggttataca gcttactatg cagctgttgt
    10621 gagctacgta agtcaataaa gggaactcga caaataaaaa aagccggatc gctccgactc
    10681 aaaataaata aacacgacac gtttattata tcacataaaa ggagcggttt gacttgatgc
    10741 aattgttacg agaggtagat ttcaaacaga caagatgtaa tgcgagagat gtgctgaaga
    10801 actttcggcg tttggagcgg atggcaggtc gctctttgat agatattaag tcgcctatca
    10861 taaccgatat gccgaaggct ccgaaatatg gaaacaaggc agaagacgcg atcatacaga
    10921 tgatggatat agaagcggag agagacgcga ttttagcggc tttgatggct ctcagtctga
    10981 ttagtcgtca gatactctac tacagcttct gtgacgtaaa taagcactct aattatgaaa
    11041 tagggcaatt gatacgagga tacggagaga agaatgtaga gaagctgaaa tccatcgcat
    11101 tgatagaatt tgcagaagca tacaaaaaag gcgtgttagt tcagtatcgt tgattttgta
    11161 gggtttttgt agggatagtg tagggttttt gagtgtttta acgtgatatt atgatagtgt
    11221 cgaaagatta gtgataggtc tgagacaaaa taataataaa aggaacatcg ttttattatt
    11281 gtttcacaat taagcttcga tagacagcag cggaaatatt aagaataagg atgtgaattt
    11341 caactccttc taaattgttc ttattatcta tcatccgttg ttgtctatta atttatgtat
    11401 tggagggtaa gaatatgaaa ttatcgattg aaggtacttc ag
//
LOCUS       DAGCWU010000087        11370 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.087, whole genome shotgun sequence.
ACCESSION   DAGCWU010000087 DAGCWU010000000
VERSION     DAGCWU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.087"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            184..1707
                     /locus_tag="MBZ71_001765"
     CDS             184..1707
                     /locus_tag="MBZ71_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBT4429720.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            1944..3284
                     /locus_tag="MBZ71_001766"
     CDS             1944..3284
                     /locus_tag="MBZ71_001766"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBT4429721.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            3437..4174
                     /locus_tag="MBZ71_001767"
     CDS             3437..4174
                     /locus_tag="MBZ71_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBT4429722.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(4339..4560)
                     /locus_tag="MBZ71_001768"
     CDS             complement(4339..4560)
                     /locus_tag="MBZ71_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBT4429723.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            4591..5622
                     /locus_tag="MBZ71_001769"
     CDS             4591..5622
                     /locus_tag="MBZ71_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4429724.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5609..7012
                     /locus_tag="MBZ71_001770"
     CDS             5609..7012
                     /locus_tag="MBZ71_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBT4429725.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            7078..7713
                     /locus_tag="MBZ71_001771"
     CDS             7078..7713
                     /locus_tag="MBZ71_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4429726.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            7762..8442
                     /locus_tag="MBZ71_001772"
     CDS             7762..8442
                     /locus_tag="MBZ71_001772"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBT4429727.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            8577..9806
                     /gene="rpsA"
                     /locus_tag="MBZ71_001773"
     CDS             8577..9806
                     /gene="rpsA"
                     /locus_tag="MBZ71_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBT4429728.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9955..11295)
                     /locus_tag="MBZ71_001774"
     CDS             complement(9955..11295)
                     /locus_tag="MBZ71_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4429729.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         3683 a         1951 c         2467 g         3269 t
ORIGIN      
        1 gctacaatga aaaagaagaa agctttaaaa acctgaaaaa agaggaggga atagggaaaa
       61 acgaactttt tgaacaaaag ttttacaaaa tgcgtgttat tatttttgtt ctgatactct
      121 ttttgataaa ataacattag agccaaaatt gttgtctcta acggaaaaga aaggaagaag
      181 aagatggaac aaacaaaaaa gaaaaaattt caaatgcctt cagcgtatac ggtcttattt
      241 attattattg cagtaatcgc tgtacttact tggtttatac cagctgggca ctatagtgta
      301 gacgatgcag gcaacatcgt cgctggatca tatgaacgag tacaacaaaa tcctcaaggg
      361 ctgtgggata ttttcatggc gccaatcaat gggatgctag gtgtaccagc gggggagcta
      421 actaaagaaa cacctgctgc aatcccaatc tctttcttca ttttggttgt tggtggattt
      481 ttaggtatta tcaacaaaac aggggcacta gatgccggga ttgcttcagt tgtgaagaaa
      541 ttcaaaggaa aagaaaaaat gcttattcca gtattgatgc tgttatttgc attaggcggc
      601 tctacttacg gaatggcaga agaaacgatt ccattctatg ctttactgat cccagtcatg
      661 atggctgtag gctttgatac agtagtagct gtagcaatcg tattagtagg gtctcaagta
      721 ggctgtctag cttcaacggt aaatcctttt gcaacaggcg tagcttcaca agctgttggg
      781 atttcgatgg gtgaaggtat tggtcttcga ttcttgatgt tcgttatttt gttagctatt
      841 tctattgtat atgtgtatcg ttatgcatca aagatcgaaa aagatccaac aaaatcatta
      901 gtatacaacc aacgtaaaga agatatgaag catttcgata ttgaagcaat cggtcgtcaa
      961 gaagtaatca cgaaaaaaca aaaacatgtt aatgtattgt tctttattac ttttggtatc
     1021 atgatcatca gtttgattcc ttggacaacc ttgaatgcga atttcacgat ttttgaatca
     1081 ttgacaaact ggttaacaaa ccttccggta ctaggtacgg ttctaggtaa aagcatgctt
     1141 ccattaggtg actggtactt cccagaaatt actatgttat tcttagtaat gtccatcgta
     1201 gtagcaatca cttatggcat gaaagagtct gaatttgtct ctgaattttt agctggtgct
     1261 tctgatttga tcggtgtagc aatcgtagta gctgttgctc gtggtatcca agtggttatg
     1321 aacaatggat taatcacaga caccatctta cattggggcg aacaaggatt aagttcatta
     1381 tcttcaagtg tattcatcat tattacattt attttctata tccctatgtc attcttgatt
     1441 ccttcaactt ccggcttagc tgctgcaaca atgggaatca tgggacctat gggtgaattt
     1501 gccggcgtcg gtaaggattt agtaatcact gcttatcaat ctgcatctgg tttagtaaac
     1561 ttaattacac caacatctgc tatcgtaatg ggtgctgtag caattgctcg tttcgatgtt
     1621 tcagtgtggt ggaaatttac cggtaaatta attgcgatat tatttgtagc catctgtgtg
     1681 atcctaggtg tagcagcaat gttctaaacc tagacaaata caggtacaag gtctatcata
     1741 agaatgaaga aaccgataaa cggctatcga taagcacgtg gataagtcaa tagctccatt
     1801 cagtgagtgt tttttctttc atcatttgtt aagaacattc tgcttgctga tggagcaggc
     1861 ttaaaacgtc attatttgag aagaagaggt tgtgtcggag gaatttgaca tgaccctttt
     1921 taacatatta ggaggataat atcatgaaaa aatttgtgac agaacaacat catgaacaag
     1981 cattagaatc attgagcgaa ttgatccgaa ttccttccgt actagatgaa gcagattcag
     2041 gacaaggaca tcccttcggt aaaaaagtga ttgaagcttt ggataaagta ttggaaatca
     2101 gtgaaaagct aggatttaga acatttaaag atccagaagg ctattacggt tattcagaga
     2161 ttggctctgg agatgaatta ttcggtatct tgtgtcatat ggacgttgtt ccagcagggg
     2221 acgaaaataa ttgggaaaca aaaccattcg atccaactat aaaagacggc tggttagttg
     2281 gtcgcggatc ccaagatgac aaaggtcctt ctattgcggc aatgtatgcc gtcaaagctt
     2341 tgattgatgc tggcgtggaa ttcaagacac gtattcgttt tatctttggt acagatgaag
     2401 aaaatttatg gcgttgttta gaaaaatata acgaaaaaga agaaggaatt acgcaaggat
     2461 ttgcgccaga tgctgaattt cctttaattt atgcagaaaa aggcttattg caagcttact
     2521 tgacaggacc aggaacgaac gagttttctg taaaagctgg aggagcgttg aatgttgttc
     2581 ctgatgcagc tccttactct ggagaaaagc tttctgaagt aaaagaagca ttgaaaaaac
     2641 acgaattcga tttcgaagaa caaggtgaag gcatcgtcgt tcttggtaaa agtatccacg
     2701 cgaaagatgc agcacaagga gtgaatgcta tttctcgtct ggcaattgct ctttcagaag
     2761 tatttgattt tggcccaatc aatttcttag gaaaattagt tcaagaaaat gcaactggtg
     2821 aagctgtcgt gggcaaaaca gaagatgaac aatccggcga gctaacgatg aactttgcaa
     2881 gtttggaaat cacaccagaa caaacaaaaa tcggtgtaga tatgagaatc cctgttacat
     2941 tcaagaaaga tgatttagta gctaaattaa ctaaaacagc agaaaaatat gggttgactt
     3001 atgaagaatt tgacttcctt gattcattat acgtgccact agacagtgag ttagtgaaaa
     3061 atctattggg gacatatcgc gatattactg gagacatgac tgaaccgttt gtatcaggcg
     3121 gcgctacttt tgctcgtacg atgaaccagt gtgtggcatt cggtgcgatg ttccctgata
     3181 ctcctgactt tatgcaccag gcgaatgaac gatgggaatt aagtagtatg tataaagcaa
     3241 tggaaattta tgcagaagct gtgtatcgtt tatgtgctaa ataaaaataa acatacaaaa
     3301 agggcggata ttcaaatgga tattccgtct ttttttattt gatgctggaa agtttgcatt
     3361 tctctcttat ttttttaagg tataatcata cttgattaag aaaaaggggt cactcagata
     3421 gaaaaggtgg gagaaagtga aaatatttag acgaatacta attggcttag tcatcatttt
     3481 aggattactc ggtggagccg gttattacta tatacagcaa aatacgtacg aaccatcgtc
     3541 agaagcgcaa gtgttggtaa aagaaggaaa aaacaaagaa gattacttat tttttgaagg
     3601 agagaaaaag aatcatggaa agtctccatt acttattttt tatcctggtg ctctcgtaga
     3661 gccagaaagt tatagtattt gggcgcaaga tttagcaaaa aacggctttt cagtcgctat
     3721 tgtcaaaatg ccacttgatc tagcagtgat gggcggaaac aaagcggata aggtcaaaga
     3781 agattttcca aattccgatt atgtgatcgg tggccattca ttaggtggcg taatggcaag
     3841 tcgttatgtc aataagaatc attcagacaa acatttaaaa ggggtattct tcatggcaag
     3901 ttatcccgac aaaaaaggaa cattgacaga atcagctgtt cctgttttat cgatcacggc
     3961 gacaaaagat aaagtattga atcaggaagc ttatcaagaa gctaagcagt atttaccgga
     4021 aagaacagag tatgtatcaa tcaatggcgg aaatcatgga ggttttggca gctatggtga
     4081 gcaaaaagga gatggagagg cagaaatttc taacgcagac cagcaaactg tgatcgtcaa
     4141 tacattggaa aactggctga aaaagttgaa ataatataaa tgggtcctat agggtccgtg
     4201 aaaaaactag tcaattaact atagatagca aatgaatctt tcaaaataag tcgggatttc
     4261 acggaacaag agaaacagat cccggcttat tttcaagttt agagaactta gtgtacgttt
     4321 ttaaagtttc gtttatattt atattttatt ttctaataaa atcgctcgaa ctggacattt
     4381 ttgatacgct ttttttacat atgtttcttc ttccggcatc acgaactgtt gatgagcctc
     4441 tggttcttgg gcaaataaaa cgattccttc ttcatcataa tcgaaaatat ctggcgcttc
     4501 gatttgacaa agaccgcatg caatacagcg ttcaggtaca agtttacata gtagtgacat
     4561 ttctaaccat cctaacttaa ggagaataat atgattgttt ttattttatc gctattttca
     4621 tcaggacaca agctgcggat tagttctctt taccaactcc ttgtgggaaa acgaacgact
     4681 tcagtactta tttatggatt tacccacgaa cttttgttta tccataacag ttttccagaa
     4741 ttgaagcagg ataagtttta tcaaattttg caaaaaattg cgcagcaagg ttggatcgaa
     4801 ataaatgaaa atgaagcaaa gctaacgcct gcaggggctg atatgttgtc cgaacatcaa
     4861 atagaacata ctggtttgcg ttttgaccgc tatggcagaa caggggaaac tagttggcgg
     4921 ctcattaaat ttgctgttca agtaatttct aatctggcaa cgggtattca agactatctt
     4981 ccagcagaga caagtccttt ttatactttc caactaaaaa aatggcttag tgaaagccaa
     5041 ttgccacgtg aaattttgat tgatactgcg tatgagagtt tggtgcagct gttttcggag
     5101 ataccagaag aagcagcaga ttttttagct aaccaattat ctggaaatga gcgaacaggg
     5161 ctgcttcctt atcagctggc aaaaaacaat gatgagtcag cggtttatct gcaacaaagc
     5221 agatgtatcc accttttgtt agcacaaatc gaaaaacggc cagattcgct ttggcacgct
     5281 ttgatcgatt cattattaca gcaaaatttc aatcaaagca tgatgatcac caagcagatg
     5341 tttatgaatg gtcagacaat tgaccagatc atggcaatcc gtcatttaaa aagaggaaca
     5401 gtaacagatc atcttattga gtgggccttg ttttttgatg attttccata cgagtggatt
     5461 ctctctgcag aaacaatcga acgtttggaa ccaaataagg attccgtgcg agagtggcgc
     5521 tttagcgaat ggaatgtaga tggacaatta gattatggag aatttcgttt gtatcaaatc
     5581 tatctattga gaaaggaagc gatacagaat gttaataaat gaattaaaaa aatattttgg
     5641 atttgattca tttcgtcccg gccaagaaga aattattcag gcgttgcttg atggagaaga
     5701 tactctcgct attttgccta caggaacggg aaaaagcctt tgctatcaat tgacggggta
     5761 tttaatggaa ggtctggtca tcatcgtgtc accgctgctt tctctaatgg aagatcaagt
     5821 gacgcaatta caaaaacgag gggagaaacg agtagctgct ttcaatagtt tactttcgca
     5881 atctgaaaga agatacgtcc tccgtcatct atcgcaatat aaatttttgt ttttgagtcc
     5941 cgaaatgttg actaatcctt ctcttttaga gcatctgaaa aaacaaaata ttgcgttata
     6001 tgtagtagat gaggctcatt gcgtgtctca atggggagtt gatttccgtc cagaatatca
     6061 acagttagga aaaatccgaa agcttttagg aaacccggtc acactggcac taacggcgac
     6121 agcaacagat ttagtagcaa gagatatacg tcatgtacta tttgatcgac caccaaaaga
     6181 gatcagacaa tcagtcaatc ggcaaaatat ctcgcttttt gttcggaaaa cgcagcaaaa
     6241 agaacaagaa cttgaacact tcatggaaag agcacaagga gcggcaatca tttattgtgc
     6301 aacaaaaaaa gaagtcgaac gattgtatca tttattcagg gaacgtttta cagttggata
     6361 ctatcacggt ggtcttgatg cagctcaaag aagacaactt caacagcagt ttgtaaaaaa
     6421 tcagctgcag tttctcattg ctacgaatgc atttggtatg gggatcgata aatctgatat
     6481 ccgttatgtg gtccattatg atttgccaga cagtctagaa aactatgtac aagaaatcgg
     6541 tcgagcaggc agagatcagg aagaaagtgc agcaattctt ttttatcaaa gtggggatga
     6601 acgtatccat tatttcttta atcaactttc tagagaacag cgacaaagtt ttgagcttta
     6661 tttggaatat gccacagagc aagctccctt tgatgagctg caaaaaaaat ggatggagct
     6721 gatccagcag tctgaaaaac cagaaaattg ggtggaacgg ctcaaacgtc aggaaaaaga
     6781 aaaagaattc cgcctgcagc agatgcttcg ctatattaat gaagaaaact gccgcagaaa
     6841 attcatctta gcttattttg gcgaaaaact ctccgaaaag ccgcagaatt gttgtgatat
     6901 tgatggagca aaagcagaac ttctgccaaa gaggaagctt ttttctaaga ctgaacaact
     6961 tcattgggaa acgattttac taaatttatt taaaaaaaac atggaacatt gattttttag
     7021 gacaagacac tttttcctat ggtataatac acacgataga gtttaggagg taaaacagtg
     7081 agcagaaaag atagacatca atctacagaa aataacgaaa cacaagaacc atgggaacaa
     7141 ccaatctacg atacagatga tgaagtttca tcaagaagtt ctcagagaca acaaaagaaa
     7201 ggaaatacat tatttctttc gctatttttg attttactgg tcttatgtat cgctattcca
     7261 gcaggtgctt atttctggat cagaaatgga tcaagcaaca atgcaacagc agcaacttct
     7321 tctgaaactt cgacatcagt agtagaaagt tctacaagta caacaaaaga ggagtcaact
     7381 gagacttcaa cagtagaatc tagtacaatc gcggaagaat ctactgtgtc tcaagataca
     7441 ggtgtaacgt cagaaagcag tattgcagca gagacaacac cgacttcctc aagtgaggca
     7501 acttatcaag caggggattc aagcagcaat gcagcagaaa caacaccttc ttcatcagct
     7561 gcagccggtt caacaacgac tgttcaagca ggcgaaggtc caaaacaagt ggctgagcgt
     7621 gcagggatca ccactgatca attgtttgaa ttgaatggct tagatccaaa caactttatg
     7681 ctttaccctg gtcaagaatt aagaattaaa taataatagc cgcgctttgc ggcttttttt
     7741 ctgagaaaaa ggagttatat catgacacaa atcagtattg caatcgacgg gcctgcttca
     7801 tctgggaaaa gtaccgttgc caagatatta gcaaaagaat tgaattatat ttatacagat
     7861 accggtgcca tgtatcgcgc agtaacttat ttagctatta cgaacaatgt atctttttca
     7921 gatgaagaag ggctagtttc gctgatcaaa aagtatccta tctctttttc gcaacaagaa
     7981 gatggacagc atgtattcat agcatcaaaa gatgtgactt tagaaatccg tcaaccagat
     8041 gtcacaaaag ctgtttctga agtctctgca catggaaaag tcagagaaga attagttgct
     8101 gtccaacgaa aaatagggga acgcggtggc gttgtcatgg acggaagaga catcgggaca
     8161 gctgtattac cgaatgcaga agtgaaaatc tttttagtag caagcgtaag tgagcgtgca
     8221 gaaagacgtt tcaaagagaa ccaagaaaaa ggaattccga ctgatcttga aacactgacg
     8281 aaagagatac aggaaagaga ccattatgat tcaacaaggg acgtctctcc gctgaagcaa
     8341 gcagaggatg ctgttcgtat cgatacgact ggcaaatcga ttccagaagt cgttgcagca
     8401 ataaaagctg ttgttttgaa aaaaggttat cttctttttt aaattaagaa aaaaaatgaa
     8461 aaacaattgt tttttcaagg aaaacgaacg aaatcgcttt attttttttg aataatcaat
     8521 taaactaaaa acagtaccgt acttacggca gaatgttggt taggaggaca agtgttatga
     8581 cagaatttga tcaaaatcaa gagaacaacc agtcaatgga agacgcaatg aaaagcgtac
     8641 aagaagtaaa agttggcgat gttgttaagg gcgaagtttt agtcattgaa gacaaacaag
     8701 tgattgtcgg aatcgaaggg gctggagttg aaggagtcgt tccggcaaaa gaattatcaa
     8761 ctttgccggt agaagatatc agcgaagtag taaaagtcgg tgatgtatta gatttagtcg
     8821 ttatcagtac gatcggcaaa gataaagaaa acggcagcta tctactttca aaacgccgtt
     8881 tagatgctaa aaaagtttgg gaagatatcg aaagagattt ccaagagggc aaggtcatcg
     8941 aagcacctgt tacaaatgtt gtcaaaggcg gtttagtcgt tgacgtcggt gtacgtggtt
     9001 ttgtccctgc atcaatggtg gaagatcatt ttgttgcaga cttttcagac tataaaggca
     9061 aaacattgag ctttaaaatc atcgaaatcg aaccatcaga aaatcgtttg atcttatcgc
     9121 ataaagcggt agtggaaaac gaaagagaag aaaagaaaaa agaaattctt tcaactatcc
     9181 atgagggaga tgttctcagt ggacgtgttg cacgtcttac tgattttggc gcatttgtcg
     9241 acctaggcgg aattgacggt ctagtccatg tctcagagat ttcacatagt catgtagcaa
     9301 aaccaagtga tgtattggct gtcaatgatg atgtaaatgt caaagtgttg tctatcaatc
     9361 ctgaacaaga acgtgtttcc ttgtcaatca aagacacaca gcctggacct tggtcagaaa
     9421 tcgatacaaa agctccagtt ggcacagtat tagacggaac agttaaacgt ttgacaagct
     9481 tcggtgcctt tgttgaagtg ttccctggag tagaagggct agtccatatc tctcaaattt
     9541 cacataaaca tattgcgaca ccacatgaag tgcttcatga aggtgacgaa gtgaaagtga
     9601 aagtattaga gatccatcca gaagaacatc gtattgcatt gagtatcaaa gctttagaag
     9661 aaaaaccagc ttcagaagaa gaaccaaaaa atgttgagtc ttatgaatta ccagaagaaa
     9721 atactggatt tactatggga gacattttag gtgatgcgtt gaaagaagaa gtagaagaaa
     9781 aagcaaccga tgattctgaa aaataattga ttttgtatct gtgactgaga tgaaagcagc
     9841 tgcttttgtc tcagtttttc ttttgaaaag cgattagtat ttcaataagg aatggctctt
     9901 cgtcaatgag tgttggtgaa gaaattcaat agaataatcg agatactcac acggctagac
     9961 agctatcttg tctagccgtg ttgttgttct ggctttagtc agttgggtag tgttcttagt
    10021 tttcttgaaa tattttcctc gatataaaaa gattcgttct cggtagtttt gaaagtttct
    10081 aaagccatag gcaattcgtt ttagtacttt gatatggttg ttcaaacatt ctatagggcc
    10141 gttggaatag ggaactcgca acgctcgttt gatttctgtt cgatatttct tgaaggtcgt
    10201 gaacactggt tgatagtgct ctggcaactc cttgtagtct tcttttagga gcgcgacgaa
    10261 ttcaggaaca tcttgtcttc tgatagcgga aagaaaaacc tggtagactt cgtagcctcg
    10321 ctttaaggag tcatcataat ataatagccg atcgactatc tccgcttctg tcagatgatc
    10381 tcgaaatgac ggacgccagt agcgatgctg accattcaat tcccacgcat tcttttgaag
    10441 tagtctccag tatttcttta agtgcttcga ccgacgatcc ccttttttaa aggtcttcat
    10501 tacttgaatt cggtggttgg taaaggcccg accgatgtgt tggctaatat gaaagcgatc
    10561 ggcgacgatt cgggcattcg gaaaacactc tttcaccaat gtgcgatagg gtgtatagaa
    10621 atctgaaacc actagacgta cttgttcacg gacttttcta gggtagcgca agaaatagtc
    10681 gcgtaaagga ttgagttgcc gattttccac aatatcaatc agttgatggg tcttgccgtc
    10741 catcatgaca aagctcatgg ctcccgagac gtttttcacc gatttgaatt catcgaaaca
    10801 caccacttct ggtaacggct ggttcaaaga aacggtttta ggccgaaggc tgcgaagtat
    10861 tcgaatgaca gtggtcggag aaatgtgctt catacgtgca atcaaagaca tagaaatagg
    10921 ctcacttaat agctcaagaa tggcttgttt gactctgcgg gcaatggagc agcgacgatc
    10981 gctaactgaa tcttctgcta aaaaagtctt tccacagtgt ttacatttga agcgctgttt
    11041 gttaatccgc acaatggtct tgtagttact gacatcattc aataagatgg atactttttt
    11101 ccagccccat ttaatgatcg tttgcttatt ttgatagtgg caatggaagc attcctctgg
    11161 ttgataggtc aatgtgccat caaaaacaaa acatgtttcg tcattaatgc gctctttgcg
    11221 aaaggcatct tcatgaaagg taagatttag gtctagaata tctagtaact ctttggtaaa
    11281 ctctgaatag gacatcttga aacactcctt tgaatggttt tcgtcgactt tattctaaag
    11341 gatttcaagg tgtctgtttg cgtttattag
//
LOCUS       DAGCWU010000088        11323 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.088, whole genome shotgun sequence.
ACCESSION   DAGCWU010000088 DAGCWU010000000
VERSION     DAGCWU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.088"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(279..494)
                     /locus_tag="MBZ71_001775"
     CDS             complement(279..494)
                     /locus_tag="MBZ71_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429730.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            complement(546..1460)
                     /locus_tag="MBZ71_001776"
     CDS             complement(546..1460)
                     /locus_tag="MBZ71_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429731.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            complement(1460..2359)
                     /locus_tag="MBZ71_001777"
     CDS             complement(1460..2359)
                     /locus_tag="MBZ71_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBT4429732.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            complement(2359..2946)
                     /locus_tag="MBZ71_001778"
     CDS             complement(2359..2946)
                     /locus_tag="MBZ71_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429733.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            complement(3110..3301)
                     /locus_tag="MBZ71_001779"
     CDS             complement(3110..3301)
                     /locus_tag="MBZ71_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429734.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            complement(3374..4276)
                     /locus_tag="MBZ71_001780"
     CDS             complement(3374..4276)
                     /locus_tag="MBZ71_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429735.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            complement(4293..4769)
                     /locus_tag="MBZ71_001781"
     CDS             complement(4293..4769)
                     /locus_tag="MBZ71_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429736.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            complement(4785..5384)
                     /locus_tag="MBZ71_001782"
     CDS             complement(4785..5384)
                     /locus_tag="MBZ71_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBT4429737.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            complement(5388..5441)
                     /locus_tag="MBZ71_001783"
                     /pseudo
     CDS             complement(5388..5441)
                     /locus_tag="MBZ71_001783"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5943..6422)
                     /locus_tag="MBZ71_001784"
     CDS             complement(5943..6422)
                     /locus_tag="MBZ71_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429738.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            complement(6441..7070)
                     /locus_tag="MBZ71_001785"
     CDS             complement(6441..7070)
                     /locus_tag="MBZ71_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429739.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            complement(7067..8443)
                     /locus_tag="MBZ71_001786"
     CDS             complement(7067..8443)
                     /locus_tag="MBZ71_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBT4429740.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            complement(8523..9785)
                     /locus_tag="MBZ71_001787"
     CDS             complement(8523..9785)
                     /locus_tag="MBZ71_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429741.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            complement(9798..10145)
                     /locus_tag="MBZ71_001788"
     CDS             complement(9798..10145)
                     /locus_tag="MBZ71_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429742.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            complement(10165..11034)
                     /locus_tag="MBZ71_001789"
     CDS             complement(10165..11034)
                     /locus_tag="MBZ71_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBT4429743.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
BASE COUNT         3169 a         2283 c         1525 g         4346 t
ORIGIN      
        1 catctttaaa gaaatacgat caaaaaacat aattcccata ttatttgctc gttcaacaat
       61 ttgaaatata tctattagtt cttctttttt cataactgct cttcctttaa cttgttaatc
      121 taacttgctt acaaatatat cctaccacat ttgtgtacta tctgccaata tttttttgtg
      181 tataaatgtg tattataaaa caaataattt tacacaaaca aaaaagagcc taactgctct
      241 tagcaataag ctctcatcac tgctcctcgt gaatttagtc agtacaatgt atttgccttt
      301 tctttgataa aatccttata cgaaaaattg ttcaaccaaa attgttttag cactaccgaa
      361 tttttgtttc catcattcaa ctccaattga ccaaatctgc tgatcatatc atcaatttca
      421 tcaattatct cttgtgccat catgccgtct tctagacgtt ctaatacgta caattcaaaa
      481 acatcccttt tcattcaaaa tgcccctttc aggttacctt ttgccatttt atgggctaac
      541 tccgcctact cagctttttt tatataccta ctgatatttg ctttcgtatt tcttggcata
      601 ccacctgctt gcaggtaatg ttcacgataa taagcatcta gctctgaaac agctttttct
      661 aatctattcg ttgcattcaa cactgagcgg tattcacgtt tttcccttaa tacacgtctt
      721 aaatggttca tcgctgatat ataaggctta tagtattcca atacagtata tcctctagaa
      781 atataggcaa ttggatttct agcttcatgg atcaattcta aaatatctgt tggatcaggc
      841 ttatccggca tctcttcttc gatttccata atactgatca agtagtccaa tccggaatca
      901 tcgactccat aactttcaca ccattctttt aggttgttca agtcatgaat aacatcttca
      961 ggatcaaact ctctaaaagc agtgatccct tggctataat attttatata ttccattgcc
     1021 gattcaaaat cctctggcgg tatgaaagct tcaatttctt cttcatcaat cgctttcagc
     1081 actattccga tacctgagtt tgaaggatat tttctcaatc cttcttcaat taaatcctca
     1141 tctattcgtc ctttttgaaa catcaccaat aattgttcta acagctgatc taaaaccctg
     1201 cgctcttcat tcagttccat tgcattttta acttcttttt caccgagttc aaagtaagct
     1261 ttgatcaact tttcttgttc ttttcgttca actaatttct tcttcaagtc attcatattg
     1321 agaatgaaat ccatgtgatt actcttttgc tgattgacaa aatcatatct ttgttccaaa
     1381 acatcccgat atttttttag ttcttcttga attatttcaa agctatcaag tacatgaaga
     1441 attttttcat tattattcat cagcccatct cctgtagtct cacgtttgtt ttcgactttt
     1501 caaaagtatt gtatttaaaa gcaattaacc ctacaaccct gctatctttg atgcctattt
     1561 ccttgttaaa aattccgtcc aatgttcctt tgactaaatt atcaatatcc ggctgtgaag
     1621 tacgcatcag tgaatcaagc tttttcttat cttttctcac ttggtaaaaa taatctatct
     1681 gagctataac ccccatagga gcattcatta cctttttcag ttgatcattt accaaatctt
     1741 tctctttaag cgtattcttg atattttcca aatagtcgtt gtactctttt ggatagtaag
     1801 tgataatccc atttcctcta aatttagact ttggtcttgg cgttgctact ggtttaaaat
     1861 caaatgtagc ttcccatata tctccgttca atccttcaga agtatcccac tcaaattctg
     1921 aatatcgttt gatcctcaca actgtttttc cttcgttcgc cacattatat ttaaaagcaa
     1981 ttaagcctgt gatatggcta tctcgtatag ctgagatgtt aaaaatacca tccatagcac
     2041 tttttactaa attatctaaa tctggcgctg ttgtcttcat aatgttatag acttttttat
     2101 agctttttgg tatctggtag taaaagtcca cttccataat tgcgcccata ggatcattta
     2161 caatcgagta aaattcatca tctaataaat cttgatcttc gataaagctc tttatttttt
     2221 tcaaataatc aagatacttt ttatcatagt aggttgaaat ccttttctct gctacgtcat
     2281 tagtttcatc agcagcattc cttcttgcac ttggtcttgg tgcagataca ggttggaacg
     2341 gtaaagttac agtccacatt agtctttttt gatctctttc aaatagattt ttttaaaggt
     2401 atcaaggctt aaaggagcta ccttttgcat cttccggatt ttatttactt gttccaattg
     2461 tttcaataga ccatcttctt ttctaaggta ctcaggattt gttatttgtt tattccgata
     2521 ctcgattaat gattgaagct ctcttactgt taaaacatcc tcaatttttg cttctttact
     2581 cgttatcttt tttagatacc ctaatactgt tggataagat ttgccgatca cttctgcgat
     2641 ttctttttta ttgtactcac caaattttcc aagcaaccag tcattcatga tcttacttga
     2701 tacttttggg tatacaagaa ttgctttttc aatggaatga ctgccattat ttctgatcag
     2761 ttgactatct ttacactcaa ctgtaacgcc atcacctaaa tcaacaacat attgccaaac
     2821 gcctcttcga ttcttattaa gagaaataat cgttccctct ttatctctat aatcctttga
     2881 taattcactt actgttgtaa ctctttcccc aatgctaaaa tgtctaatat cattattgtt
     2941 ttccacttat tatccctcca atattgctgg ttatctcttc ctctgacaag gaactatata
     3001 tcatataaag aattttaaca ttattcggaa tgagtgtaaa gaaactttat ataaaaagag
     3061 tgatttagtc taagaccaaa tcactctttc ttttttatta attatattgt tatctgatag
     3121 acctattagt aaatgcacgt cttattgttt gatcattctc gatcaattct tctaacaact
     3181 ctgaaactgt tactttctta cctcttgctc tttcttcttc tttagctcgc ttttctaaat
     3241 atttaacaga agctattttt aaagtcacat actttctaac tttttcttta tttactacca
     3301 tcagctatca tctccaaggt gtcacaccta tatattattg gtgtgacacc atttgtttat
     3361 tataactttc tggctattta gccaatttaa tcgttaatgc ttcgtttgct gtagaatctc
     3421 caaaaacttt accgtcttca taaactacat gaactggcaa atctgcaccg tctgaatctg
     3481 gtttagcagt accacgatac aaaccaatta ctctttttcc tagttctctt gcaaccgtaa
     3541 cttttttatc ctgatccata gattcaactg aattgaataa ggtaacatta atcatttgct
     3601 tttcagtatc caatttaaca gttttgatag ctttgaagac ttcagtgtta aaccgatcag
     3661 ccatagaacc agaaacagat tcaaacgatt tgactttttc aggatcaaaa tcaattttat
     3721 ctggtagttc cgcttcgaca gtagaacttg attcagttgt actttcttct gtagctactt
     3781 cttttttcgc tgattcgata atctttttcg ttgatgtttt atttattttt acatctttat
     3841 agtattcttc tccatcaacg ttaaaaacca atgtcagctt gtgtacaggg ctattctctt
     3901 taactgtata aaacacttca aattgtgatt gagacggctt aatagtagcg agaacgtcgt
     3961 ctgatgcttt cttgccatct tccttcactg tcagcgaaac atttttataa ctcgttacat
     4021 tttcaaatgt tccatctatt actacccttc ccaaagaatc tggagcataa ctattattaa
     4081 ctcccgatat accaaattgt ttatcgttac ttccaaaaat cttagtggca ttaaacacaa
     4141 taaaggttac aatcacaatt ccaatgccta tgttacgcat aatcggatta tttgtttgaa
     4201 attgaccaat aattatagcg attgccccaa aaaaagcaag aattaaacat aaataaccta
     4261 acagggttaa tagcatgatt catcacaact ttctattctt tatttttctt tttggttttc
     4321 gtttgttcaa actcatgctc atttagataa gtaatcaata aatctcgcat ggatttgcta
     4381 tccttactta gcgtatcaaa cgcttcttgt aagtcagcat ccttctctac agcattaaac
     4441 caatgcttcg ttccaaattc aaaaagatta ctaatcactc ttaacggttc ttgatcagta
     4501 ttcgtattaa gccattcaag tatccgttgt tgagctaatc ctaatttact ttcgtcttcg
     4561 tgatttgtac gtgcttcata gattttagtg tgtatatcat caaaatagtg gattcgttct
     4621 cttagcgctt cacgttgttc gtacatagcc cttttatttc taacttcttc gtctattgtt
     4681 tcaatctttt ttcttaacag tatttgaagt gataacattt ctgagacaga caaaaacact
     4741 ggaattttct tttcttttct ttttggcatt tttagcactc ccttttaatt cttttgtttg
     4801 atcaatgaaa cgttctttag aataaatccc aagatcattc cacagacaaa tccgattaca
     4861 tgtgacaaga catttgcctc gcctgattca aaagtagata ccaataaaat gccgattatc
     4921 aataatacaa aaacacttag taataaatgt ttcttgctat caaaacggat gtatgcaatc
     4981 gcaaataaag caatccatcc aaaaatacct ccactaagcc ccatacttac gatgttgctg
     5041 tgtagcagaa aaggaactaa gcttcctaga aatcctgata tgattaaaac gatcaataat
     5101 gttaaccact catactgatc attcagtgct tcttcaagaa ctactaagaa caataaatta
     5161 gaaattaaat ggtacaagtt aatatgagaa acgatagaaa tgaatatatt cgggaacaag
     5221 tgtccttcat acaaaccacc taaagcaacc atttcctttg cactcaatgt atttccaatt
     5281 atgctttttc caatctgcaa aaaatcaaac aggaaaatca atacacaaac aaaaactaca
     5341 gtcgctacta ggtatcgact actatttttg tttttttctc tcattcgcta cttccttcgc
     5401 tttagtaact gccgattctc catattctct aaaaagagtc gatgttagct ttcctctcgg
     5461 aatattgtat ttttttaaaa tttcagttgt tgaaagattt ttttcaaagt cttcaataat
     5521 ttgtttatga agatttattt tagccaaatc attggttgtc aaaacaggtt ctttagcttg
     5581 aatatcgcct tgacgaatta acctttgaat tttatttttg ctttttaaaa aaggatattt
     5641 ttcaattaaa gggtcaattg cattcccttc atcaatccac ttacctattt cttgataaac
     5701 tctcttttga aattttttcc tcagctcaat cgggttcgga aaaacattgt gcttaaaatc
     5761 gtgtgatact aggctctttg atttgcctaa ttcatcagcg acttcttgca ttgtaatttt
     5821 gttttcgata tagcattgga caagagcatt atatttttta gggttattat tatttattgt
     5881 catgttctta cctacgttca ttgctaattt tttggtattt ttctgtttgt gtattgcgat
     5941 ttttaaaagg catttcctaa tacgttaaat tttgataatc ggttcaattt tactgcttta
     6001 tcaatttctg cctgtgacca aggttctatt aaacgcattt ggtacgtcac tagcagatcg
     6061 acatgttcat attcaaactt ttctattccg taaatctgat aaaatatttt ttcttcgttg
     6121 ttactataag gaatcactgt tccgatctta aatatgtttc gtgtgttgtt tacaccaatt
     6181 tcatcagtaa tcttgtgtct atattctaat aacacgtatt tcttatacct gcttgtcaga
     6241 tcaggtgaat agttaacaga ttgacaaacc aagtcactgg ttatacgtgg atcacctatt
     6301 ctgttaattc tcgtgttata gattccgata ataatatagt ttttatcttt atattctata
     6361 gtttctccca cttctaactt ttgattaaag cctgttctat ttgcttcaaa aagttctagc
     6421 aatctttttc acctctactg ctactgccaa acaaatttag ctttatcatg ccatgtttca
     6481 gacattactt ttttaccatt ttcgtcaaat atcaccgctt cccactttgc tgttccatcc
     6541 ggctgtttac aaatatctgc aaaccatagg atcgtttttc gatcgttgaa tttccttact
     6601 ttcttctcaa atttattgat ctgaactcta ttttgattgc tgttccaacc tactaatagg
     6661 cggttatctg attctgtacc atgaaaacca atgggaacga tgtgggttct atcaaatgct
     6721 tcttttcctt ctggatagag cattggtcta agtttcctac gtttcaagtc atctcttgct
     6781 tgataccgcc atgccgattt tggattgaat ttaatagcta tttgatgctt gcttttgtca
     6841 tggtaaaaat atgcgacatc ggcttcagct ccaagcaact tttctcttgc actatttgat
     6901 tcaaacggtt tattcacttc taaaaccttc acccctaaaa tgccatgatc aagcatctgt
     6961 ttaggagtta gtaccgcttt tttatctctt ttcttcgctc gtttcacaac tacttcatct
     7021 gtatatttat atggagtaag ccatcttaaa aaagaactga cgatgatcat ttatgcacct
     7081 gtttttttgc ttttaacatt ttttgcatga taggtgaagt acttccattt atttgtttct
     7141 tttcagattt atcagttgtg atcactacgt tttctgagcc ttttggatca ttagctttag
     7201 gtttggtttt agatgcaatc ggctgtttat tattcgtgtt gtttgaggtt tgcggttcag
     7261 gatcagcttt tcctaatgcc cttaaaactt tttctagttt ttctgcttgc agtttttcta
     7321 gtgttttagc tccctgctcg actttttctt ctttgttctt ttcaagctta atagcaaacg
     7381 cactcttctt ttcaattgat ggctctttag atgatggatt tgctaaaggt gtgttcaaat
     7441 ttcttttagc agattttctt acaggagctt taccaattcc accaaaatcc tcgtcttcac
     7501 cgccctcacc acgagtttca gctacgattg ctcggtaatc aacatattct ggttctggca
     7561 tagtatttct aaggtgattg aagattgttt caatttcttt gttactgatg taagctccct
     7621 gcgctctaac catacttcca tcaacaagct taataagcat atctcccttt ggacttagac
     7681 tttctccacc gccttcatct aatacaatcc tactttctaa atcacttgct accataaggc
     7741 aaacacgtga cggaaaattg gctttgataa taccagtgac aactgttgat cgaggagatt
     7801 gtgttgctaa gataacatga atacctgctg cccttgccat ttgtcctaat tgaacaatca
     7861 aaccttctac ttctttatgt gttccaacca actgtgaata ttcatcaact aataagatca
     7921 catagggaaa tctatctaat ccattttttt cggcatattc gttatatagc gtgatgtttt
     7981 gtaaccctgc tttttccatc agtttgtaac gttcatgcat caagatcaca agatattcaa
     8041 aaaaatcata agcttcatcc atatctgtaa ttggatcagc caacatgaac ggcaattttt
     8101 tgtatcttcc aaattcaacc ctttttgggt ctacaattcc tatttttaat tcatcaggcg
     8161 ttgagtgaca cattactgta acaagaattt cattcagtaa tactgatttc cctgatcctg
     8221 tcgttcctgc aaccaacatg tgtggcattt cgctgatgca aaaatgagca attgtgccat
     8281 caggcttaat tccaaaaata gcatctgttg ggtctttagc ctttgtgttc aacatattga
     8341 cactgaattt tccattaagc tttatattag gttctttatc tagcaaaata gattcagctt
     8401 tttcggctct ctttttgact ttctctaata atttcatagc catttctatt cctcactttt
     8461 ctcggtactt ttctgtttgt gaactggtag tttgtatttt ctgtttctat ttgtgatgtc
     8521 ttctaaaaca cgctattgat cattgatggt acatcaattg gaatacgatc attttctcct
     8581 aatggtaaag taatttgaat acgccctgcg ttgatccgca cgattacacc tgatatatgc
     8641 aagtaagttt caatttcttc tttcattgag cttagttgaa tttttctggt acgagataaa
     8701 gtaaacaaat attcaataga tgtattccca acgtgcatat ctgttagact tgcaggtacg
     8761 tcagcagaag atagataaag ttctaaagat tgcttttgag tttcggcata ttttctagct
     8821 ttttcggttt ggttttggat atttttggaa ttatcggcaa agagattgat tggaaaagaa
     8881 aattgtgaat ttgaaactgc gattgaagat gttgctcttt ccgtttcttt gtttttggta
     8941 cttactcttt ccttacgttc tctttcaata tttactgatg ctcggaaaat gaaataatcg
     9001 cctgaaactt catcttcttt ttcaccaaat ttggctcgtc cgcttgttgc tcttgtcagg
     9061 atcgacccta aatcttgaat ttgtcgtaaa actgtttctc taactttctt cccttttggt
     9121 tgcgggattt ttacgatata ttctcgaata tcttcattga cacttagatc acgtcttgta
     9181 tgaacagaaa ttttgatatt tttaagcgtt gatagcacat cggattctaa ttgcgttttg
     9241 tttcgttttc tttttttgtt gttcgtttca tctcctataa tcttgtttgg atcatacttc
     9301 acatccatag cttctagagc ttctcttttc aaccttctac tttccatatc atcaaaaaat
     9361 ggtctttgtt ctccatagtg atgcctgaac atcaaagtaa ccaaatatcc aacaaagaat
     9421 accgttcctg cttctaatac taaaatagag atgtctacca agtgtagctt tgcaaaaaag
     9481 ctttgtacat tatatgaata acttaaataa ttgggaaaaa acgcattgag taactcttta
     9541 aacatctctt ttaatcctaa atcatctgtt ccgttaacat catttaacga gacaagaata
     9601 cttactaagg tatctacaag cagttgtaca acctttgtta cccaatgcaa aatatatatg
     9661 ccaattgcac caactaccaa tgcacgtcca accagatata ctgatttaga aaaactggct
     9721 acaggataat atttttctcc atcgcttcgc ttatattcct ttaattcaat ggatttttcg
     9781 ctcatctgaa tatctcctta ttttctttct gattttggtt catcgtcact tatgctgtcc
     9841 tcacacatct gcgtatcttt aggaatgatt actctgattt cgtctaacat ttctctctca
     9901 atgatcatcg ccccttttgt actaacagtt ttctctgata attcaacaca tttccctatt
     9961 tcagaatctg gatcaaaaat agcctgttct acgtcaaaat gattcttagc tacatacacg
    10021 atactcattg tttctaactc tctagcaact gaatatttac ccttcttcag aagatagtag
    10081 ccatttcctt tttttattcg atttgcaact gatttatcta atccattgat gcttattgtt
    10141 tccaattctt caccttctca atttctaatc tcttacccaa gtaccatttt gatagttgat
    10201 gttgattcct ttttgattgt taaatacata aacattaagg tttaccgttt ttccattatc
    10261 gttaactgag aaagcttcga catgagaacc acgaggaact aattcattcc ctttataaat
    10321 tggatcaact ttatacaata ctttttcatt cttattttta tagattgcat cacgtacaat
    10381 cgtttctgag taagccatcc ctccattctg atcatttttc ccaacatttt gcgctctcgt
    10441 tccaagtgtt aaattgtgta cctccatatc tcctcctagc gaccatgcta atgaatgact
    10501 tttgttatag agataacctt tatagccttc tagctgagtg atttcattgt ttgaatcttt
    10561 gccgacccaa gtatctctct cttgatcaaa tactccatcc ttgtattctc ctgcaatatg
    10621 gacagaactt ttgaaagatg gacgttccac atttttagat gagtgagaat ttacagcatc
    10681 ataggtgatc aacgcttcaa catctcctgc tcttccaagt tgatctaaat ctgcataatt
    10741 tacccaaaag gtttgactgc ctttttctga taatgcttca tggtcttctt ttgtcagtgt
    10801 atatttccca tcattaatgt ttacgatatt atttggatat ttgtttggat cataacgtat
    10861 ccctaaaaat tcttttgatt cactgtctgc tttattacct acctgaacac tacttttgat
    10921 ctgaaaatca tgttcctgag tactgttatt ctgaaagtaa cccttgccac taagaccaac
    10981 cgcaactaaa accaaaattc ctaacaacga tcggctaaac ttcttctttt tcataccacc
    11041 attcctttta ttgtgttatc ataataagat aaatatataa taaactatat tgtaaatttt
    11101 gtcaaatgta tttttattta ttcccactat aaaccttgat atatagccaa tttaaaaaag
    11161 gatggcaaaa aattatcaag ttaaaaaaat aaattttgaa ccaaattatt tttaataaaa
    11221 tagctagttt ttttatcaaa aaaaatagag cttttcagct ctattcagac tgtagacaaa
    11281 ctcctaaatt tggagtgttg tttgcagttt tttgttttat act
//
LOCUS       DAGCWU010000089        11264 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.089, whole genome shotgun sequence.
ACCESSION   DAGCWU010000089 DAGCWU010000000
VERSION     DAGCWU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.089"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            226..1485
                     /gene="lysA"
                     /locus_tag="MBZ71_001790"
     CDS             226..1485
                     /gene="lysA"
                     /locus_tag="MBZ71_001790"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBT4429744.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1472..2272
                     /gene="dapF"
                     /locus_tag="MBZ71_001791"
     CDS             1472..2272
                     /gene="dapF"
                     /locus_tag="MBZ71_001791"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBT4429745.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2275..3156
                     /locus_tag="MBZ71_001792"
     CDS             2275..3156
                     /locus_tag="MBZ71_001792"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBT4429746.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3153..3869
                     /locus_tag="MBZ71_001793"
     CDS             3153..3869
                     /locus_tag="MBZ71_001793"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBT4429747.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3875..4570
                     /gene="dapD"
                     /locus_tag="MBZ71_001794"
     CDS             3875..4570
                     /gene="dapD"
                     /locus_tag="MBZ71_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBT4429748.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4586..5587
                     /locus_tag="MBZ71_001795"
     CDS             4586..5587
                     /locus_tag="MBZ71_001795"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBT4429749.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5584..6945
                     /locus_tag="MBZ71_001796"
     CDS             5584..6945
                     /locus_tag="MBZ71_001796"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBT4429750.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6905..8056
                     /locus_tag="MBZ71_001797"
     CDS             6905..8056
                     /locus_tag="MBZ71_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBT4429751.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8031..9200
                     /locus_tag="MBZ71_001798"
     CDS             8031..9200
                     /locus_tag="MBZ71_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4429752.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9375..10754
                     /locus_tag="MBZ71_001799"
     CDS             9375..10754
                     /locus_tag="MBZ71_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4429753.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11035..11264
                     /locus_tag="MBZ71_001800"
                     /pseudo
     CDS             11035..11264
                     /locus_tag="MBZ71_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3750 a         1958 c         2507 g         3049 t
ORIGIN      
        1 ttcatcacta aatagtgttg aatttatgtt tttatgtata aatagcttct aatagttcgg
       61 gtattttgtt tttataacca tttttattct tatttttaaa aaaatatttc gaaatatact
      121 agacaattca gaaaattttg tttaatatta ttccaataaa attcaaatct ttatcaaatg
      181 aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac agaattgata
      241 attggcggag tcacagcaag cacattggca gaaaagtata agacacctct gtatgtgtat
      301 gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt atcgaaacgt
      361 tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat gctgcattta
      421 gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta taccgctttg
      481 caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac gccagaggaa
      541 ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt gatggaagtt
      601 gaattattag cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat gcttcggttg
      661 aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat tgattcaaaa
      721 ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat aaaagaaagc
      781 agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt tgatgttact
      841 gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga ccaacctctt
      901 tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca gccgatccca
      961 gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga aaaacgaaaa
     1021 ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga agctggtaca
     1081 acactataca cgattggttt catcaaaaag acaccacata aaaaatatta ctttgtagat
     1141 ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga ttgtgattta
     1201 gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa aatgtgtgaa
     1261 tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg tgatctttta
     1321 gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa tcgagcgaca
     1381 cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg cagacaagca
     1441 ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata atggctgcgg
     1501 aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat tagccgcttc
     1561 tttatgcaca aaagaaaaat atgatataga cggattgatc gccgtaaaaa tcgatccgtt
     1621 ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa atggcattcg
     1681 ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat tcaacgtaga
     1741 gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt attgcagcgt
     1801 agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa cttatccaat
     1861 aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt tatttttagg
     1921 aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag gaagtctgga gaaaaagagg
     1981 tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact ttgtagaagt
     2041 agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt ggacacttgc
     2101 ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga aaatttcagc
     2161 agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg aggatgcgat
     2221 acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat gaagatgata
     2281 aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt agattatgct
     2341 ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt attagtttta
     2401 ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat ccttcaactg
     2461 acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac gaataatacg
     2521 aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc acttttggtc
     2581 atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt tacagcaatt
     2641 gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac aggcatgtcg
     2701 attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg tttgaaagaa
     2761 gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga agaattcttc
     2821 ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg aagcggtgta
     2881 ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa agatacgcag
     2941 gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact gatccatgcc
     3001 ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt agacttacct
     3061 tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa acaattagct
     3121 cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt ggcagtggac
     3181 agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc cttcttttac
     3241 ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa ctgcttattg
     3301 atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca tatcaacttc
     3361 cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata aaagaacatg
     3421 ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg gtgatgaatc
     3481 aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg attgaaaaac
     3541 atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt caaacaatcc
     3601 aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga gaagaaaacc
     3661 aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat gtattgtttg
     3721 ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga atctttgctg
     3781 caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat tacaaattag
     3841 aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa tcatccgcta
     3901 cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa ccgagccaat
     3961 cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt ttggtgacta
     4021 tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa tcgaaatcga
     4081 aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta atgctcgtat
     4141 cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg tgatcatgat
     4201 gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg atatgggtgc
     4261 tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag gagctgtctt
     4321 agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg gcgtactggt
     4381 aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag tcgtggcagc
     4441 aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa tacctgcacg
     4501 tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg ctgcgttacg
     4561 cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg tgggggcaac
     4621 cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc cagtaggacg
     4681 attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca agaaaaagaa
     4741 acacactgta caggaattga cagaaacttc atttgaagga tatgatttag cgctattttc
     4801 tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg gactatttgt
     4861 catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg ttccagaggt
     4921 caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt caaccattca
     4981 agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag tcaattattc
     5041 gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc atcgtacgca
     5101 aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga ttccggaaat
     5161 cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa atgagacccg
     5221 taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg tgccaatcga
     5281 aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga cggaagaaat
     5341 tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag cagcacataa
     5401 atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga ggatcagaaa
     5461 agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca tacgcaaagg
     5521 agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag cggaggtggc
     5581 aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc tcatgaagag
     5641 caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt gatcgttgtt
     5701 tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact ttatttgatt
     5761 tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat ttgtgatcgt
     5821 ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga actagcacgc
     5881 atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag tagaggtgaa
     5941 tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga cgcaaaagac
     6001 ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca ggaaatcaca
     6061 aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc gaaccctgca
     6121 ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat actggcgaag
     6181 atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat gatggctgat
     6241 ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga actgagagag
     6301 atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt tcaagaggct
     6361 gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac aaaaatatca
     6421 aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa ggattttctt
     6481 tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg gaaagcaatg
     6541 agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat cgacaatatc
     6601 ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac gaaagaatta
     6661 aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt gatctctgtc
     6721 gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc gatattgaac
     6781 aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa tctgatcatc
     6841 ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg gatggtgaat
     6901 acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg ggtttcatga
     6961 attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcgaggccg ttatccatga
     7021 aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa cgattgcttt
     7081 tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt ttgcttctaa
     7141 gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc ttttagggtt
     7201 gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac tgatctatca
     7261 gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat tgttggataa
     7321 atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta agggagaagt
     7381 tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta tcattaaagg
     7441 ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg cagctgtacg
     7501 atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga atcatctaat
     7561 gaaatttggc caaatccaag gtggaacgat ccgaaatgtt ttagctggag aagttattgt
     7621 atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga ggggattaga
     7681 ccatttagca gaacagtttc actctacagc tactatttct tttcgctata atgacggcta
     7741 tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa acaaactaca
     7801 tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc aacaatatcc
     7861 atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc cgacgttcga
     7921 ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc tttttacaga
     7981 aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata atgatgaatt
     8041 tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata cgacaattcg
     8101 atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa ccggattttc
     8161 caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac ttttctcact
     8221 ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt cagcaacagc
     8281 gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt gcaacggaag
     8341 cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt attccagcgc
     8401 ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg atcccgatag
     8461 atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca tttgaacaat
     8521 atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact gggacgacat
     8581 tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg gtatttgtta
     8641 taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc atagctactt
     8701 atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg atgactggtt
     8761 ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt aaagtccatc
     8821 aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa gcgctaataa
     8881 atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga gactatctgg
     8941 tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca ttttacttgt
     9001 tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta gaattagcgg
     9061 aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga gaaggttata
     9121 tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa cggatcaaag
     9181 actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt aattttgcgg
     9241 tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc gtttcttttg
     9301 attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa agaatattca
     9361 aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct catggagggc
     9421 atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc acgctatttg
     9481 aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta gaagacaagg
     9541 taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa tccaatgttc
     9601 atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta
     9661 ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg
     9721 gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc
     9781 gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa
     9841 tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg
     9901 ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg
     9961 agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg
    10021 caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag
    10081 aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa
    10141 aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga
    10201 atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca
    10261 aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac
    10321 atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa
    10381 gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag
    10441 gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag
    10501 aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc
    10561 taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag
    10621 cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag
    10681 gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac
    10741 catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat
    10801 caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta
    10861 ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat
    10921 ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt
    10981 tatggttaaa tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa
    11041 aggcgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg
    11101 gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc
    11161 tctatcaata aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca
    11221 tacacgcgta aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DAGCWU010000090        11220 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.090, whole genome shotgun sequence.
ACCESSION   DAGCWU010000090 DAGCWU010000000
VERSION     DAGCWU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.090"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            62..685
                     /gene="lexA"
                     /locus_tag="MBZ71_001801"
     CDS             62..685
                     /gene="lexA"
                     /locus_tag="MBZ71_001801"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBT4429754.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            865..1083
                     /locus_tag="MBZ71_001802"
     CDS             865..1083
                     /locus_tag="MBZ71_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429755.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(1372..1884)
                     /locus_tag="MBZ71_001803"
     CDS             complement(1372..1884)
                     /locus_tag="MBZ71_001803"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBT4429756.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1898..4195)
                     /gene="recJ"
                     /locus_tag="MBZ71_001804"
     CDS             complement(1898..4195)
                     /gene="recJ"
                     /locus_tag="MBZ71_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBT4429757.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(4337..4780)
                     /locus_tag="MBZ71_001805"
     CDS             complement(4337..4780)
                     /locus_tag="MBZ71_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBT4429758.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4781..5569)
                     /locus_tag="MBZ71_001806"
     CDS             complement(4781..5569)
                     /locus_tag="MBZ71_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4429759.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(5566..6507)
                     /locus_tag="MBZ71_001807"
     CDS             complement(5566..6507)
                     /locus_tag="MBZ71_001807"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBT4429760.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(6684..7271)
                     /locus_tag="MBZ71_001808"
     CDS             complement(6684..7271)
                     /locus_tag="MBZ71_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBT4429761.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(7492..8799)
                     /gene="obgE"
                     /locus_tag="MBZ71_001809"
     CDS             complement(7492..8799)
                     /gene="obgE"
                     /locus_tag="MBZ71_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBT4429762.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            9102..10112
                     /gene="gap"
                     /locus_tag="MBZ71_001810"
     CDS             9102..10112
                     /gene="gap"
                     /locus_tag="MBZ71_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBT4429763.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(10197..11132)
                     /locus_tag="MBZ71_001811"
     CDS             complement(10197..11132)
                     /locus_tag="MBZ71_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4429764.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3126 a         2511 c         1967 g         3616 t
ORIGIN      
        1 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
       61 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
      121 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
      181 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
      241 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
      301 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
      361 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
      421 tatgctgaca attcgtggag atagtatgat caatgctggg atattggatg gtgacaacgt
      481 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
      541 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
      601 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
      661 actttatcgt agtcatatct attaattgct actaattaaa agaaaacttt ttaaaaagag
      721 tttgagacgt tggtctcaaa ctcttttttc gcccgggttc ttcttctttc aaatttttat
      781 cacttccctc tttacaaacc gaacaaatat tcgtatacta aatatacgaa caattgttcg
      841 cataaattaa agaggtgaga attcatgaaa tcaatgagac gtcttggctt agtattaact
      901 gttttgtttt taggtattat tattggtaat ttcggcatac gtccagcttt acttgtgttt
      961 gttccgttat ttactttgtg gttcatgttg tgggatgaaa agaagtaccg tcaatctgag
     1021 cggcgcagaa ttcatgaaga tcatttctat catgagccct actacagatc caggcaatca
     1081 taataaggat tgactgctaa ttattcaaaa gacaagaagt taaactttta cgggggtgtg
     1141 acaaaaatcg gtcacatcct ttttttcgaa taaacggtgt tctaaagcca gtttttcggt
     1201 attaagtcaa attgcggaaa aagtgatttt ttaaacttct actggtcttt gcttgtttcc
     1261 atttataaaa aaatagcgac cctcaaaagt cagatagaga atctgacttt tgagggtcgc
     1321 tttaaaatcg cttctttaag aataacatca agaatagctt cttttttctg cttaatattc
     1381 cattaatgta atgatgtcat atcctttgat tttgtcgcgt ccatggagat ccatcaattc
     1441 gatcaaaaat gcgcagccta cgactacacc gcctaattgt tcgatcagtt cgatcgtcgc
     1501 tttgattgtt cctccagtag ctagcaaatc gtcacaaatc agcacacgtt gacctggttt
     1561 aatggcatcc ttatgcaatg tcaatgtatc tgttccatat tctaatccat aagtcacttc
     1621 gatggtttct cgaggtaact tccctttttt gcggactggg gcaaaaccga ctcctaactc
     1681 ataagcaaca ggacatccta cgatgaatcc acgggcttct ggcccaacta ccatatcaat
     1741 tcgtttttct tttgcataat ttacgatttg tttagtagct tcccgataag cgtcaccatc
     1801 tgccatcagt ggggaaatat ctcgaaacac aattcctttt gacggatagt cgggaatact
     1861 ggcaatataa tcttttaaat ccactctttg tttcctccta ggttgttaac catttcttta
     1921 tggtaggaat atcgctcaaa agcaaaaatt cctcaacttt gattttattg agccgtcgct
     1981 ggtataatct gctctcagtc aaaggatgac tttctgggct agcgactttt tgtaaaacac
     2041 cgttctctat tgtaacaaat ttcaattcaa aaaacacctg tatcataaaa attaataatt
     2101 tttcagggat acgcaaatat tgagctattg cttttatttt atgacgtata tcaattcgtt
     2161 cttgagaatg gatcaattta aacaattttg cgtactgttc tcttgtgcct accccattca
     2221 aataggcctc atcaggtgaa atccccatca gataaacacg attgaatgaa taagaccgaa
     2281 aaacttcttt cagatccgct aaatcatctg gacaatccat aacaactaga gagtttggtt
     2341 ttcttttttc aattattgcc tttacagaat ctagtccttc gaaaacaatc gcagcttcct
     2401 ggataatctc aggcacgaat ttcaacgtcg aagaatcaaa tgctaaataa aggctacctt
     2461 ccgacgttcg gctctgttca cggaaacgtt ttgcccgcca atcaaatact tgcagaccac
     2521 ccacagaaaa atcagatacc atcagctgcg gtttcttacg cccgttccat tcattgatcg
     2581 aaagctttcc taccacatct acctgattat tcagcagttc ttcttcttga gtaccaaaac
     2641 caaaagcgac cgcatcaagc tgactcgatg catcggaaag gacaaatttc agatgctgtt
     2701 ggtttgctcc gattcttttg atattttctg cttggacttg tcgaaataaa aactgaggca
     2761 aaggattatc cgtaccaaat ggcgccaata gtttcagctc atcaattcgc tctactgtta
     2821 cttcatttgg cagcaatact tcatcaattc tcaactcagg ccctctcatc aagtcaattt
     2881 gatgatcgac tatatattgg ttcatcttct cctgtagtat agtgacattt tcacttggca
     2941 tcgttaaacc gactgctgca tgatgtcctc cgaagtaagt aaataaatct cgcatctggt
     3001 caagcatttc aaaaagattc aaggcttcaa tactacgtcc agatccttta gctgatccat
     3061 cttcttttaa agtaaggaca agcgttggtt tacccgtttc attcattatt ttcccagcaa
     3121 caatgcccaa aacgccttca tgccatcctc ggtttaccaa tagatggata tgattctcct
     3181 tattgaccat cgctaatgct tcttgtgtta tctgctctac gattgctttt ctttcttcat
     3241 tgatctcatt caattttttt gcttgaacgg atgcttcctc ttcatcaaac gtcgcaagta
     3301 acgagacggc aggacgtgga tcgcccatcc gaccgattgc atttagtctg ggagcaatcg
     3361 aaaaaccgat cgttgtttcg tctgcgtccc tcatttttac gccgctttct tcaaatagcg
     3421 cctgaagtcc tatccgttct gaatgatgaa tggcatcgat tcctaatgaa acaaggattc
     3481 gattctcatc agtcaatgaa actaaatcgg cgatcgttcc tatcgctact aaatccaaaa
     3541 attctgcagg cggttcttcc aataatgcag aagctacttt gaatgctact ccgactcctg
     3601 ccaaatctgg aaatggatag tgccccgctg gatgacgagg gtgaacaatc gcataagcgt
     3661 ctggtaaatt ctcaggtagt tcgtgatgat ctgtaatgat cacgtctaca ccagcttgct
     3721 gagcgtatgc taccgcatca tttcctgcta ccccgttatc aacagtgatg atcaactgga
     3781 ttccttcttc gatcttttct tggtataccg attggtttgg tccataccca tgctcaaatc
     3841 gatttggcag gaatacttct acttctgcac caagtaattc cagtgtttct ttcattaccg
     3901 ttgcactggt gatcccatct gcgtcataat caccgtaaac gaggattttt tctccactga
     3961 ttacagcttc ttggattcgg ctgattgctt tatccatatc gtgtatcaga tacggatcat
     4021 gtaattcttg ttcctttgga tgtaaaaatc ggttgatatc cttttcctca tggtaacctc
     4081 tttgccacag taacttaccg acaaatggag ataatttcag ttcacggatc atttggagaa
     4141 aggattcaga aggctctgtc tcgcttaact tccaatcaaa atttgcttgt ctcactttct
     4201 cactcctaac ttagttatta tagcaaagga aatcagcaaa aaaaagcaga aagaaagaat
     4261 aaaaacagat cagatccgct agacagatgt tcagcaggac ctgatctttt gtacgaccat
     4321 ttatgaaata tccctattag tcaaaataat tgaagctttg tttgggcgat tgagtttttt
     4381 gttcttccgg cgctttcaat tgactgattt gtgcggtttt cgcttgtaac tccgcctgat
     4441 aagcagcttc caactccgca cgttcgtttc taaactcgcg ttgctgttct tcttttgctt
     4501 cttcaatttt cttttgaagt tctgtttgat attctgacat ttcctgagtc agctgtttga
     4561 tttgcttttt ttgctgccat agtgttgccg aagcagtcaa aaatacaatc agtgcaccaa
     4621 tcagcgcaga accgatgatc actaagatca atggcgcaga aaaggaagtg aagccaaaac
     4681 ttacaggaac atttttattg ttcaatacag caaaaagaac aataatcaaa actaaaacaa
     4741 atccaagaat cacattgctt tgctttttca attccttcac ctactttttg ttaaaaattc
     4801 cgctggctaa ataatcacca atcgttggaa acagcgtata aaattttgat gcaacttcta
     4861 aaataaacgg ttgattgatt tcccgttttg gatgtctcat attgcggacg atcatttttg
     4921 ccaacttggc aggttctaaa acgatttgtc cgactttttc caaatagctt ccgcttggat
     4981 cagccttatc gaagaaattt gtctcgattg gacctggatt cactgtcgta acagctacgc
     5041 caagtggttt caattctaat cgcaaagcgt tggagaagcc aaggactgca aatttcgtgg
     5101 cagaataaac cgttgatttt gcagttgcca tttttccagc catagaggcg atattaatga
     5161 tatgaccttg tcttctttct gccatatcga tcgcaaattt ttgtgtcaaa accatcattc
     5221 ctaagatgtt gacttcaaac atatcgtatg cttttccaag gtcgaatgta aggaagtctt
     5281 cgaaaatccc aaaacccgca ttgtttacca gaatatcgac gcgcccgact tcttccatca
     5341 ccttttcata taattcgtcc acgctttcag gattgctgac gtctaccttg aaagcataag
     5401 cctctttgcc gctcaagcgt ctgcactcgt ctctgacacc ttctacaaat gccattcttc
     5461 gtgcgcaact tataacgatt gctccttgct ttgctgcttc ataacagatt tgtgcgccca
     5521 gaccgcctga actacctgtt actaaaacaa ctttatcttc taaattcatg cctcttcctc
     5581 ctcgcgaaat ggaatctcaa tgctatcaaa atctttcata atcgtcgtgt tttcaaacac
     5641 ttcttgtgct tctttttcaa gctctaaagc tgcttttcct aaatatctcg cactgatatg
     5701 agtaagtagc agccgttttg cctgcgcttc ttttgcaact tcagcagctt gatgagtcgt
     5761 ggaatggaaa tagttgtgcg ccattcttgc ttcatcttta ttaaatgtgc tttcatgtac
     5821 cagaatatcg ctattttccg caagcacaac ggaattatgt gtttttctcg tatcgcctaa
     5881 aatagtcacg attcgtcctt ttttatctgg acctacgaaa tcttttccat tgatcgtccg
     5941 cccatcttct agttggatcg tttcaccttg cttcagtttg ccgtacagtg gtccagctgg
     6001 aatacctaat tcttttagac gatccacctg caattctcct ttatgatcat gttcaaccac
     6061 acgataacca aaactgtcga tcccatgatt tagttttttt gtataaacag aaaattgctg
     6121 atcagtaaaa atcggggctg tttcatttag ctcaatgaat ttcagcggat aacttaaacg
     6181 cgtttgcgaa atacctaaag agacttttat gtattcctct atcccttttg gcccgtaaat
     6241 ctctaatggc gtatccccac cttgaaatga ccggctgctg atgagcccgg gaagtccaaa
     6301 aatatgatcg ccgtgtaaat gtgtaatgaa aattttaccg atttttctcg gacgaatagt
     6361 cgttcgcaaa atctgcattt gcgtgccttc gccacagtcg aacaaccaaa cttcattgcg
     6421 ctcgtctaaa agtttcaatg ctatacttgt cacatttcta tgttttgcag gaacacctgc
     6481 acctgttcct aaaaattgta attccataat ctacctgact ttctttaagt tcctctttaa
     6541 ttttagggta aaaagaaggg ataatcaacc gtaagtcgag aatgggttca aattaaaaat
     6601 aacgttctaa agcgatagcg ttttctttga aaattactat aaaaacagca gcgaacccgt
     6661 ttgagatcct ctgctgtttt ttattaattt gaagatccag aactttcatt gtcgttggaa
     6721 gtttgcggat gtttgtcctt ccaagtcttt tgtgcagctt ctttcgcatg attttcagct
     6781 tctttcgcat catgcacatc tttttcgaca tcttcataat ccaagcgagt aatttctccg
     6841 cctaattcca tcattaccaa ttgattcagt gggcgattgt cctcttcatc agcaatcaag
     6901 atcagtcctg tagctccttc accgatttta tttcctacaa agtcgaacac ggtctgggca
     6961 tcttttattt ctttggcatc ttgtgtcgca ccgatcattc ctccggcaaa ccatcctaat
     7021 aaaatcccta gcggaccacc taatacacca accaacattc cgattaatcc acccgtagaa
     7081 gatttgttat taccggtaaa gtcgataaaa tcttctattt tgaattgatg tgtcccatta
     7141 ggatcatggc tgacaactgc catctgttct cctttaatcg aacgaacagc atgtaacttt
     7201 ttgatttctg aaaatgcctg atatgcctgg ctttctactt gaaaatttaa aatgattact
     7261 cgtttgctca ttaaaaacgc ccctttcata ccttcttgtt atttatttta cctttaattg
     7321 taaaataaga aaagaaaagc actctttttt tccaataaaa tagggctgtg acaaaagtcg
     7381 tatcacagct cctttttccg aataaacggc gttcttgaag ctgatcactt ctgtcacaac
     7441 ctcccttaaa tctgaatcta tattttttgg tatttgtttt tatttgcttg cctattcgac
     7501 aaattcaaat tcaaactcac cgatacgtac aaggtcgcca tctttcgcac cgcgtgcccg
     7561 aagagcttca tccacaccca taccacgtaa ttgacgagca aaacgcatca ctgtttcatc
     7621 atgatcgaaa ttcgtcattt ggaataattt ttcgatcttc tcaccagata agatccacgt
     7681 tgcatctgaa tcacgatcga tttggaactc aggaccttct ggattgaatc catagtgtac
     7741 agtttcttct tccagttctt cttcgtacaa tgggaattct ggcgttactt ctaacaaatc
     7801 tgctgtagca ttcaataacg cgtccaatcc ttgtcgagtc actccagaaa ttggaaatac
     7861 tgggatgtca tcagcaaatt catcttcttt ttccttgttc agctgttctt tgaatttcac
     7921 tagattttcc ggtgcatccg gcatatccat tttgtttgcc acgatgattt gcggacgctc
     7981 tagcaagcgt aagttataag tagataattc tttgttgatg gcaagatagt cttcataagg
     8041 atctcgtcct tccatcccac tcatatcgat cacatgtaaa attactcgtg ttctttcgat
     8101 atgacgaagg aattgtgtac ccagcccaac accttgcgaa gctccttcaa tcaagcctgg
     8161 caagtctgct gcggcaaaac ttctgccatc acttgtagta accattccta gattaggtac
     8221 gagcgtcgtg aaatgataag cgccgatttt tggacgagca gatgaaataa cggatagtaa
     8281 agtcgatttc ccgacagatg gaaaacctac aagtccaacg tctgccaata ctttcaattc
     8341 tagttcgatt tttcgctctt gtcctggttc accgttttca gcaatttccg gtgcaggatt
     8401 ccgaggagag gcaaaacgga tattccctcg tccaccgcgt ccaccttttg ccacaactag
     8461 tgtttgacca ttttcaatta aatctcctaa aagagcacca gtctccgcat ctcggacagt
     8521 ggttccttgt ggtactttaa cataggtatc ttctgaacca cgaccgtgca tccctttact
     8581 catcccattt tcacctggct gcgctttgaa atggcgattg aaccgaaaat ccatcaatgt
     8641 acgtaaccct tcatcgacga tcaagatgac atcgccgcca cgaccaccgt ctccgccagc
     8701 cggtccgccg tcaggaacgt atttttcgcg acgaaatgca accattccgt ctccgccctt
     8761 gccagctttc acatcgatcg ttacttgatc taaaaacatg gacataatta agtcctccta
     8821 ttattcattt atttatttga ctgcctgaat ttccacaata aacaaaagtc ttacttattt
     8881 tatcggtgaa atctgatttt gtctatcatt tcagcaaaaa gattgtttac cttcttcttt
     8941 ttttatcatt tggtaaggtt tccaaccttt tttctgctac caagtataat tttttgacaa
     9001 aatattcttt gcgtagcaga ataacagtgt attaattgta ttaattgtat taattgagga
     9061 tgccgtttca cagcacccct aataaaggag gaacttttga tatgacagta aaagtaggta
     9121 ttaacggatt tgggcgtatt ggccgtctgg ctttccgccg catcaaagaa gtctcagatg
     9181 atattgaagt agtagcaatc aacgacttaa caagtccaac tatgttagca caattattgc
     9241 aatttgactc tactcatggt acttatcctg gcacagttac tgcaacagaa gactcaatcg
     9301 tagtggatgg cgaagcaact cgtgtttatg cagaacccga cgcaagcaaa atcccttggg
     9361 caaaagaaaa cggtgtagat atcgtactag aatgtactgg tttttataca tctgaagaaa
     9421 aagcaaaagc gcatttagat gctggtgtca aacgagtagt tatttctgcc ccagctggtg
     9481 ctatgaaaac catcgtctat aacgtcaatg atgatacatt agatgaaaac gacaaaatca
     9541 tctcagcagg ttcatgtaca acaaactgtt tagcacctat ggcttatttc ttaaataatg
     9601 aatttgggat cgaagttggt acaatgacaa cagttcatgc ctacacttcc acacaaatgc
     9661 tcttagatgg acctgtaaaa ggcggtaatt tgcgtgctgc tcgttcagct gctgacaata
     9721 ctatcccaca ttctactggt gctgcgaaag caatcggctt agttatccct gagctacaag
     9781 gaaaattaca aggacacgca caacgtgttc cagtcgttga tggttcatta actgaattgg
     9841 tttcagtatt gaaaacaaaa gtaactgctg atcaagtaaa cgaagcaatc aaaaaacaca
     9901 cagttgacaa tccttcattc gggtatgatg accgtcaaat cgtttctagc gatgtcattg
     9961 gaacaactca aggttcgatc tttgacccaa cgcaaacaga agtaacaact gctggtgatt
    10021 tccaacttgt taaaactgtt gcatggtatg acaatgagta tggatttact tgccaaatga
    10081 ttcgcttact agaaaaattc gctaacttgt aatttgatag atcaatcgat aaaagaagcc
    10141 tttgactgga tccagtcaaa ggcttctttc ttttcatccc ctacttttaa actcgttcaa
    10201 tgaacatatt ggcttaaaaa ttgttggact acttgctgat atttttcagg atctgtcttg
    10261 tatgcttccg catgctcggc tccttttaca acatactttt cttttggtcc cttcgttgca
    10321 cgatagacct tatctaacat ttcaaatggt acaaatgtat cttcgtctcc atgtataaag
    10381 agcattgggc gctggttttt cttcaattgt tccactgcat ttgcttcacc gaaaaagtag
    10441 ccagcacgga cttttgtcac tagactcgtt acttgcataa gcggaaatgc tggaagacta
    10501 aacatatcgt ctagctgata agcaagctca tcatgaacgg aagaataacc gcaatcttca
    10561 atgattgctt tcacattatc tggtagtttt tctccgctag tcatcataac tgttgctgct
    10621 cccatgctga caccgtatag aacgatttct tgcgattttc cattgttttc tagcactttg
    10681 tttatccact gtacatagtc tttacgctct ggccatccaa atccaatgta atccccttcg
    10741 ctttttccat gtcctcttgc atcaggaaca aggacattgt aacccatatc atgaaacatc
    10801 tttgcgtaat tggtcatcgt ttcagcgttt cccatatagc catgagccac aagagctgtt
    10861 ttttcacttt tcgtttcagc aggaagataa atcgctgata atttcagacc gtcttttgaa
    10921 gtgatggtcc aatcgcttcg gttttctttt gaactaaacc atttatgtgc atccgtttct
    10981 ttggatgtct gtgttgtacc aggtgtatct tcgtctaaaa agtctttctc agaaggcaca
    11041 acagcataat tataaaaata attccctgca aacactaacg caacaatcag agctaaagcg
    11101 acgcctaaaa ggattttcat ccaaagtttc ataaatcatc catctcattt ctcttttttc
    11161 atataacctt gaattagtat aatcaaagaa tctctactat gcaatgttca tttttataac
//
LOCUS       DAGCWU010000091        11202 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.091, whole genome shotgun sequence.
ACCESSION   DAGCWU010000091 DAGCWU010000000
VERSION     DAGCWU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.091"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            6..305
                     /locus_tag="MBZ71_001812"
     CDS             6..305
                     /locus_tag="MBZ71_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4429765.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="MBZ71_001813"
     CDS             378..1739
                     /locus_tag="MBZ71_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBT4429766.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="MBZ71_001814"
     CDS             1761..2021
                     /locus_tag="MBZ71_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429767.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="MBZ71_001815"
     CDS             2035..2787
                     /locus_tag="MBZ71_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBT4429768.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="MBZ71_001816"
     CDS             complement(2881..3486)
                     /locus_tag="MBZ71_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4429769.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="MBZ71_001817"
     CDS             complement(3539..4513)
                     /locus_tag="MBZ71_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBT4429770.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="MBZ71_001818"
     CDS             complement(4891..5610)
                     /locus_tag="MBZ71_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4429771.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="MBZ71_001819"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="MBZ71_001819"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBT4429772.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="MBZ71_001820"
     CDS             7161..8558
                     /locus_tag="MBZ71_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4429773.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="MBZ71_001821"
     CDS             8639..9079
                     /locus_tag="MBZ71_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4429774.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="MBZ71_001822"
     CDS             9227..9553
                     /locus_tag="MBZ71_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4429775.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="MBZ71_001823"
     CDS             9554..10303
                     /locus_tag="MBZ71_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429776.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELIGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="MBZ71_001824"
     CDS             complement(10383..10838)
                     /locus_tag="MBZ71_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBT4429777.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3555 a         1960 c         2298 g         3389 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg ataggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt ta
//
LOCUS       DAGCWU010000092        11123 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.092, whole genome shotgun sequence.
ACCESSION   DAGCWU010000092 DAGCWU010000000
VERSION     DAGCWU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.092"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(1..1044)
                     /locus_tag="MBZ71_001825"
     CDS             complement(1..1044)
                     /locus_tag="MBZ71_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBT4429778.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(1034..1525)
                     /gene="coaD"
                     /locus_tag="MBZ71_001826"
     CDS             complement(1034..1525)
                     /gene="coaD"
                     /locus_tag="MBZ71_001826"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBT4429779.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKNL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(1522..2082)
                     /gene="rsmD"
                     /locus_tag="MBZ71_001827"
     CDS             complement(1522..2082)
                     /gene="rsmD"
                     /locus_tag="MBZ71_001827"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBT4429780.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(2201..2539)
                     /locus_tag="MBZ71_001828"
     CDS             complement(2201..2539)
                     /locus_tag="MBZ71_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBT4429781.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(2523..2960)
                     /locus_tag="MBZ71_001829"
     CDS             complement(2523..2960)
                     /locus_tag="MBZ71_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBT4429782.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(2950..4074)
                     /locus_tag="MBZ71_001830"
     CDS             complement(2950..4074)
                     /locus_tag="MBZ71_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429783.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(4095..7523)
                     /locus_tag="MBZ71_001831"
     CDS             complement(4095..7523)
                     /locus_tag="MBZ71_001831"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBT4429784.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(7549..8712)
                     /locus_tag="MBZ71_001832"
     CDS             complement(7549..8712)
                     /locus_tag="MBZ71_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBT4429785.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(8715..9017)
                     /locus_tag="MBZ71_001833"
     CDS             complement(8715..9017)
                     /locus_tag="MBZ71_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBT4429786.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(9131..10903)
                     /gene="recQ"
                     /locus_tag="MBZ71_001834"
     CDS             complement(9131..10903)
                     /gene="recQ"
                     /locus_tag="MBZ71_001834"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBT4429787.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         3039 a         2434 c         1899 g         3751 t
ORIGIN      
        1 tcatttcatt ttctctagat agtcaagcgc ttcttgtact gtttttactg gtacgatttt
       61 cattgaggaa ttcaattttt tagcggcagc ttttgcttct tcgtagttgg tttttatttc
      121 aggatattct ttcttaacat cgtctgaaat cgtatcatct ggtgcaaaga aaatctctac
      181 gccagcttta tccgcacttg ccactttttt atcgattcct ccgattcggc caacttctcc
      241 ttcactgttg atcgttccag ttcctgcaat ttgcttacca tgtcgcaagt ttttaccagt
      301 gacttgttca tagatttcta aagtgaacat caatcccgct gatggcccac cgatagagcc
      361 agaattaata cgaacatctg tatctgattc gatctcggta tggtcagtaa gcgtgatacc
      421 tattcctgcc tttttatctg ttggtaactc gatcagatca ccagaagctg ttttttcttc
      481 accattatgc aaataactga tttcgattgt ttgtccaact ttttggcttt ttacataatc
      541 cataaatgct tctgtacttt gaaagctttt cccgtctact tttgttactg tatctccaac
      601 tgaaagcttg ccaaagaaat tcgatgtttt ctctacactc atgacatata cgcctttgaa
      661 ttccatttta taaggcttgt ctgcaagttt taacgcttgt tcgattgctg cattttggga
      721 agaatccata taatactgtt gcatttgatt atattcttca tttgtcgccc caccagtcaa
      781 ttcttcttca ctgatcaatt cttcaaaagg ggtcatttta gcttttaaag ctaagaaacc
      841 tgtagcttgc ctgataccaa cagaggtaag agagaaagac ccagaacctt tatcttcctt
      901 atcattgacc gtaattagtt ctttcagatc gatcgtggct cccggtgatt ctacataata
      961 cggcaaggga actactacaa atccaatgat caacattcca aatatgataa tcagtactgt
     1021 tttcaaccat tgtttagctt tcatttgatt cacgctttcc agctaaagct tcgttgatca
     1081 ctggcggtaa gtagctgctc acatctccgc caaagtgcag tacttctttt aaaatactgg
     1141 aactcacgtg actatattct ggttttgcta ataagaagac agtttccaat tcatcatata
     1201 gatgatgatt catctgagca atctctcgtt catattcaaa gtctttgata cttcgtatgc
     1261 ctctgatcaa cgcatctgca ccaatctctt ttgctgtttc taccgtcaat tgattttctt
     1321 gatgcatgac ttttacatta ggaagatgag aaacagcttt tgtgatcaat gtcattcttt
     1381 cttctggggg aaacaaattt ttctttgaag tattgataaa aacaccaatt actacctcat
     1441 cgaataattt agccgcacgt tccaccgtat ctaagtgacc ttttgtaaaa ggatcaaaac
     1501 ttcctgggaa taatgcacgt ttcattgatt tccctctttt ctatagatag ttacggccgt
     1561 tatgccataa atctgttttc ttatttgatg gaatccagcc acagtttctg gcaattcgac
     1621 tgttttatcc gtttcgcata cgataactgc ggttttagaa aaaagtccca gttcttccat
     1681 tttatggatt tgatcgatta ttttttgttt tgcatatggc ggatcaagaa acaagtagtc
     1741 gaattgttgt ttctccgctg ctagccgttc gattgctttg tccgcatcaa gttttattgt
     1801 ggtaaatttc tctggttctt tcgtaatttc gatgttttct ttgatgattt ttagcgcttg
     1861 gtaatttttg tctacgcata ccgcatgact gcagcctcta gatactgctt cgatagctaa
     1921 accaccgctt cctgcaaaca gatccaatac tgtctctcca tcaaaatatg gtccaatcat
     1981 attgaatatg gattctttta ctttatctgt agttggtctg gtgttcgccc ctgctaaact
     2041 ttttaaacgg cgcccgccat aatttcctga tattatccgc atctactcac tccttagaaa
     2101 tggtactggt actagtatac caaaaaaata cttggacaat gatcaaaaat cgatcgttat
     2161 ccaagttatt ttttatttaa aaactgctca taagagattt ttaaagatgt tcagctaatc
     2221 ggatttctgt atttagctct tcaatttcga aaccttcgtc ttttaatact gttttgtttc
     2281 ctattttttc ggcaaaattc atttcgatat ccggtcggta agaacgttct acgtttcgaa
     2341 caaaatgtaa tttacggatt cgttcttctg tttcttccaa tgtctcttca tttaagtaaa
     2401 gcacgacata tttcattctt cttgagacat aatgcaacaa gccgaacttt ttcagattct
     2461 ttaattgttt taagctgtag acccatacga tcagcccgcg tcgttttgtt atttcaaatt
     2521 cactattgtc taattgcatg acaattacct ccgcatccaa agtgtttttt cgtttcaaaa
     2581 aacgggctac ctgcatctac tttgatttca ttagaaactt cttcggcaag tacttggaca
     2641 atttgatcca acagactttg cagctgtgtt tcactatatc ggaaagctgc aacatgctca
     2701 tccatatcaa gctgtctttt tgcttttcga acagctcgtt ttttttcacg ataatcaggt
     2761 gcatactcac catatgccgc tacattttca aatgcttctt tcttagacaa gaacgcttct
     2821 ttcaactggc aaacttttgc agattcttcc attgactgtt tatgttcaag ataagaagcc
     2881 atcgttttac tcttttttat catttcagcg acttggaggc attgatcctc cagttgaaac
     2941 agctcttcgt taacgatcac tgctatcact actttctccg ttttcttctt gcaataagac
     3001 taacatattt tctttagaaa aagcaatgcc tacagaatcc gttcccgggt ccatgaaacg
     3061 gtcatggttg taaggatcgc tgtaccaata taaaaacgaa aaagtagcgt caatcgccgg
     3121 atgaataaat agcccactag tattttctaa tttttgttta ctgattaact tctccaattc
     3181 atcattcgtc agtgagaatt tcactgtcgt ttgagcactg cctagcgatt tttgccaatc
     3241 agccatacgt tcctctgaca atatttcctc aggccttgtc aaaaaatcat gtagtagcag
     3301 ctgactgtgg ttttgaaagg caagtgtttg attgtatatg ggaagttcgt cttctcttct
     3361 aagcatattt aataaagtcg tactttttcg ttctttaacg cgattgattg attcccaatc
     3421 agccgattta tctgcttgat aatgtggaag gccttcacca aacacttgat atggcaatag
     3481 tttcaaaaga ctatctttat ctaaatacac aactgaaaat aggccgtttg ttgtttgatc
     3541 aaaaaataaa atagcaaaag tgtcgttatc aaaagcaatt agcggacgat aattcatatc
     3601 ctcttccatt agttcgattc caacttctgt atcatggtac tcaaaagtga aattagtata
     3661 gatagtagtg attttcgtaa gatcttgcat cgtcatccct aaataaaacg gcgcgatatc
     3721 agcaggccct gtttgcagta ctttaacagt tgtgactctg ctgtcttgga tgttcgcttc
     3781 aaatgtgaga tgactagctt tattttcata atatcttgtt tcaaaaccaa atccgctagt
     3841 taatcgatca taggcttggc caaaatcctt ttccagctga tcgatcggtt ttccgatata
     3901 agtagaaaaa ccagttgctt taagctcctg gtatttccac gttgtcattt ggtttgatat
     3961 ttgttgatca gagttttcct tcacttcaag ttttggcgga aacaagactg gttccatgta
     4021 gaatgccaca ataactaata gcagaatcag ccaaaacaaa cccagtcttt tcatgtttcc
     4081 ccacctttcg cttattattt ctcagtcact tcaatcaaaa ggtcaccaga cgaaatagtg
     4141 tctcctgaag acacatatac atgtgcgact tctccgtcaa aacgtgcttc gatggccgtt
     4201 tccattttca tcgcttcagt aatcatcaac gtatcacctt ttttaacgtt atcgcctttt
     4261 ttcactaata catccaaaac agaaccagac atcgttgctc caatttgttc tcggtttgtc
     4321 ggttcagctt tcaatcgggt ttggaccgta ctgatgatcg aattatcttt gatagtgatt
     4381 tcacgtcttt gtccattcaa attgaagaac agtacgcgat tcccttcgat atcgggttct
     4441 ccgatttcat ctaaacgaat gataagcgtt tttccgcgtt cgatttgtac attgattgtt
     4501 tcacccaaac gtataccttg gaaaaatgtt ggtgtgtcca gtaatgtaac atcgccaaat
     4561 tgattatatg ctttttgata gtccaaaaag acttgcggat acatcaaata gcttaatact
     4621 tcgtcttgtt ttggttcata gccaattttt tctgccaatt ctttttttac ttcgttgaag
     4681 tcgactgatt cagccaaaag acctggacgt tcattcaatg caggtctgcc tttaagaata
     4741 atcttttgca attttttagg aaatccgcca acgggttgcc ctagttctcc ttggaagaaa
     4801 gtcacaacag attcagggaa acttagtgtt tcgccttttt cataaatatc ttcttctgta
     4861 agatcatttt ggaccataaa tagtgccata tctccaacta ctttagatga tggagttact
     4921 ttaacgatat cgccaaacat caagttgaca gtatgataca tttgtttgat ttcatcccag
     4981 cgatgaccta atccgacagc ttttgcctgt tgctgtagat tcgaatactg tcctcctggc
     5041 atttcatgca tatatacttc tgtttcagga gcattcaagc cgttttcaaa cggtttgtaa
     5101 tacatgcgca catcttccca ataatggttt aatttctgtg cattttcaat cgtgatttct
     5161 ggcagccgtg gaccattgac cagtgcataa tacaagctgc tcatgctagg ctgactagta
     5221 ttcccactca ttgcactcat tgccacgtca acgatgtcta ctcctgcttt tgaagcagca
     5281 gaataagtga tgattccatt cccgctggta tcatgagtat gcagatggat tggcagatct
     5341 gtcgtttctt tcaattcact aatcaaacga taagctgcct gcggtttaag cagcccagcc
     5401 atatctttga ttgcaatgat atgcgcaccc agttgttcta gttcctttgc catgtcttta
     5461 taatactgaa cattgtattt tgcccgactt ggatcgttga tatcgccagt atagcaaata
     5521 gctgcctctg cgattttacc agtatcccgt acagcttgga tacttttttc catttgtgga
     5581 gtccagttca aactatcaaa gattcgaaat acatcgattc cttgcgctgc tgcttctttg
     5641 acaaattcca ccaatacatt gtcaggataa ttagaatacc caacggcatt tgaacctctg
     5701 aaaagcattt gcagcaacgt attaggcatt aaagaacgaa tttttctcaa ccgttgccaa
     5761 ggatcttcat tcaaaaagcg gtaagccaca tcaaatgttg ctccccccca catctcactt
     5821 gaaaagagtt caggaagacc ttctccagtc aaacgtgcaa tctgcttgaa atcctttgtc
     5881 cgaacacgag ttgctagcag actttgatgg gcgtctctaa atgtcgtatc cgtcaacaac
     5941 aaattttcct gtcctttgat ccattcgacc aaagcatcgg cacccttcgc atctaataca
     6001 ttcttagccg ttacgtaatc agttcttgtg attagatctt taggcatacg tggttcttca
     6061 taaaatggtt tttcgccact ttcgatacct ggaaaaccat tcacagtaat ttcaccgata
     6121 tatttcatcg ttttgtttcc gcggtctcgt agtcgaggaa attcaaataa tgttgctgta
     6181 ctatcaatga aggttgtttt tgcatttcct gattggaatt caggatgtgt gatgacattg
     6241 agcataaatg gaatgtttgt tttcactcca cggattctaa attctttcaa gcaacgttcc
     6301 attttctgaa tggctgtttc aaaggtagca ccatgcgtac atacttttac tagcaatgaa
     6361 tcaaaatacg gtgtcactac atagcccgcg taagcatttc cgacatccag acgtacgcca
     6421 aaaccaccag gtgaacgata cgtatcgatt ttccctgtgt ctggtaaaaa gttattcaac
     6481 gggtcttctg tagtgattcg gcattgaatg gctgaaccat ttaatttaat tccttcttgc
     6541 tgaggcaatc cgatttcttt atgcagatcc aaaccttgag caatcaacag ttgcgttgtt
     6601 acgatatcaa tatctgtaat catttctgtg atcgtatgtt ccacttggac gcgagggttg
     6661 acttcgataa agtaaaaatc atctccttca acaagaaatt ctactgtacc tgcgttgaca
     6721 tacccgacat atttcatcaa ttggacagct gcctggcaga ttttttgacg ttgctgttca
     6781 ttcatagaga cacaaggcgc tacttctact actttttggt gacggcgctg aacagaacaa
     6841 tctctttcaa aaagatggat gacgtttcca tgcgtatccc cgagaatctg tacttcgata
     6901 tgcttaggat tagcgatgta tttttcaaca tacacttcat cactaccaaa ggctgctttt
     6961 gcttcgcttt tcgcacgttc gtagccctct cttgcactct tttcatcgtg agctacacgc
     7021 atgccgcgtc cgccgccgcc tagagctgcc ttgatcataa tcggataacc gtattgtttc
     7081 gcaaactcca aggcatcgtc tatagaagcg attggtccat ctgtgcctgg aatcgaagca
     7141 atccccgctt cgatagctgc agcttttgct ttgattttat ctccaaaaat atctaaatga
     7201 tgcagtttgg gtccaacaaa aatgatgcct tcttcttcgc accgttgcgc aaaattcaag
     7261 ttttccgaca aaagaccgta acccggatga atagcgtcag ctccgcattc tttcgcgata
     7321 gaaatgatcc cttcaatatc tagatacgca tcaatcggtt tttttccttg tcctactaaa
     7381 tatgcttcat ccgctttgaa acggtgtacg gaatattcat cttcttctgc atagattcca
     7441 acagttttaa ttcctaattc tgtacaagca cgaaatacac gtacagcgat ttcacccctg
     7501 ttagcaacta aaactttttt catcgaacca ctcctacatc tttttttatt ataaacctaa
     7561 gctcttgcgt ttttcatccg cactgatgtt gaggacaaag ccgacacaaa tcgaaagcat
     7621 cagcaaactg gagcctcctt gacttaaaaa tgggaacgtg atccctgtca aaggaataac
     7681 gccagtaatt cctccaagat tgacaaatac ttggatcaaa aacattgcgc cgatcccaat
     7741 gcacaaaagc gaattgaacg gatcttttga acggatacct acaaggacaa tgcgcacaat
     7801 cataaagaat agcaacgcta atatcaaaat ccccataatc actcccagtt cttccactac
     7861 gattgcataa ataaaatcgg tttgtgcttc ttgtaaaaat ccttttttct ggatactgtt
     7921 acctaatccc ctgccaaata gtcctccgtt gaacatggca tagtagccgt tgactagttg
     7981 atgcccttta tttaattcgt ctgagaaagg attttggtaa atagcaaatc gattatagat
     8041 atactgtaaa cgtccgggta agatctttcc gtttgtgata ttgatcagcc agatcgtaaa
     8101 agtacttaga caaaagcctc ctactcctac aatcaaagta tacacataat tgactccgct
     8161 ggctaaaagc agaaccaaaa taatcaaaaa gataactgcc gcattaccaa aatcgggaag
     8221 aatagcgact aatgcagtaa gtccgataac cattgcaagt ggacgtttca cagtcccaag
     8281 aaaatctttt tgtacactgt tttgtctttt agataaagtc agagacaggt accagataga
     8341 aatgattttt aggtattcgg caggttggat cgagaatcct gcaatttgca gccaaccttt
     8401 cgccccattg atctccttac caaaaaacac aacaatcagc agcaaaatcg tcaaaactgc
     8461 gatggctgcc atgatcaaac gctggttttt caaaacatct gttttcattt tataaagtag
     8521 tgcgatagca atcaagctaa gcacccaaaa aatactctgg ttaatgactg atgcggacgg
     8581 attttgtccg ttttctaata atagatacga cgtagaacta tataccatga tcaatcccaa
     8641 tacacataaa attaaatagg gaatcaagat gctatagtct aatagatgcc tttttttcac
     8701 tttcttaggc aagtctattc ctccttgcca acttcttttt tatcataaat ttcttggtaa
     8761 acctcactat ataactgatt caattctcgt tctagctcac ttaacatcag ctggccctcg
     8821 ttctttccga ttactcctat acggacagca aaaccgactt gccgggaaaa gccgtacatc
     8881 tgtgtatcta ccacttcttc gaaggctttg cattgtgaga tacagagact gttgttttga
     8941 tttcggatta atcgtttgat ccgttctgct tcatcattta atacttggat cgcaagtttt
     9001 ttcgacaaag aatccatctg tgcttacctc ccattctaat tccttaatta taacatattt
     9061 gattttcata aggagcaaaa aaaacagatt taaccagttg tttttattca tttttacgta
     9121 ttcgttcttt ctacttatcc agaacgttag agtccgcatt ttttatccga gataaaaagg
     9181 cttcgccata tttatcaagc ttgctttggc caactccttt tatctgaagc atctccaatc
     9241 tgcttgatgg acgtattcgg ctcatttctt tcaatgtgga atctgaaaat acgacatacg
     9301 ggggaacgcc tgcttctgat gctagatccg ttcgcagttc ccgcagcact tcaaatagtg
     9361 tatcggtttc ttcatccgat agctgctgga ctttcttggg ttcctttcga taaacagatt
     9421 cttgtccttt caatacttgt atccctcttt ctgttacttt caaaacagga tactcaccat
     9481 tgatggtcag aagatagcca ttcgatatca aatattcaat taactgcatc gtttcttttt
     9541 gcgattggtt tttcatcagt ccataggtag ataaatgtcc aaaacctaaa gcttgcagct
     9601 tttgttcttt cgatcctgct aagactttca tcagcatttg tttaccatag ttctcaccca
     9661 tacgttttaa gcaagaaaga accatctgtg cttctgtcgt tacttctact atttctctgt
     9721 catctaaaca gttaccacaa cggttgcacg gactgcttgt ctcgccaaaa tagtttagga
     9781 tgtattgctg aagacaggac tcaatataca cgtattctgt catcgctttt aatttttcat
     9841 attctttttg cttttgccct tcttctcttt gggactgctg gataaaaaac ttttggactt
     9901 ggatatcttg aggagagaaa agcaggatgg cttcactgga aagaccatct cttcccgccc
     9961 gaccagcttc ctgatagtat gcttctagag agccaggaat ttgataatgg ataacaaaac
    10021 ggacattgct tttatttata cccataccaa atgcatttgt cgctacaatc agctgcaagc
    10081 gatcgtataa aaaggcttct tgcatttctg ttcgctcatt ctcatttaga ccaccatgat
    10141 atctacccac ggaaaaatcg aattttttaa gcaaatgata gagacggtct acttcttttc
    10201 tcgttgctgc ataaatgata ccagattgat ttttgttgat ctttaaatat tcgattagat
    10261 attgttcttt tttttgatct ttaattactt gaaatcgtag attttctcgt tcaaaaccag
    10321 tctgaatatg gttgttttct ggaattttta aaagtctttt tatgtcagca gctacttgca
    10381 cagtcgctgt agctgtcaaa gcaacaatta caggacgatt tggcagttga tctagctggt
    10441 tcgccatctg taaataactt gggcggaaat catgccccca ctgagagata caatgtgcct
    10501 cgtcaatagc gatcatacgg acgttccatc gcttcattgc ttggataaaa tccggcatga
    10561 taaaacgttc aggagcgata taaagtaaac ggtactgctg tctatcgatt tcttggaagc
    10621 gtctagctgt ttcgtaacca tctaacgagc tattgataaa agtggcgggt atacctaatt
    10681 gattcgccgc gtccacctga tccttcatca aagagattaa aggggacaca accacagtaa
    10741 gcccatctaa tagtaaagct ggaagctggt aacagattga tttcccactt ccggtaggca
    10801 tgattcctaa tacattttcc tgatcaatga ctttctggat aatttctttt tgaccaggtc
    10861 taaactcatc atagccaaag tattgtttta ataagtcgtt catcttcccg ctcctttctc
    10921 ttgccatttt acttcgattt tcttcgaagg acaagttgat ctcctttcgt gaaacattat
    10981 cttattatga aacaaaaact ctctagaaat aagtttttta aaagtctttt aaacgtcttt
    11041 cggcgtattt tctgaattta tgtccattga caaagatgac ttctccacag aaaagagttg
    11101 gacaaaaaaa gaaaccagct att
//
LOCUS       DAGCWU010000093        11051 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.093, whole genome shotgun sequence.
ACCESSION   DAGCWU010000093 DAGCWU010000000
VERSION     DAGCWU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.093"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(271..1539)
                     /locus_tag="MBZ71_001835"
     CDS             complement(271..1539)
                     /locus_tag="MBZ71_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBT4429788.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(1770..2507)
                     /locus_tag="MBZ71_001836"
     CDS             complement(1770..2507)
                     /locus_tag="MBZ71_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4429789.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(2574..3143)
                     /locus_tag="MBZ71_001837"
     CDS             complement(2574..3143)
                     /locus_tag="MBZ71_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBT4429790.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            3271..4143
                     /locus_tag="MBZ71_001838"
     CDS             3271..4143
                     /locus_tag="MBZ71_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4429791.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4485..5357
                     /locus_tag="MBZ71_001839"
     CDS             4485..5357
                     /locus_tag="MBZ71_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4429792.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            5354..7312
                     /locus_tag="MBZ71_001840"
     CDS             5354..7312
                     /locus_tag="MBZ71_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4429793.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(7563..8414)
                     /locus_tag="MBZ71_001841"
     CDS             complement(7563..8414)
                     /locus_tag="MBZ71_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4429794.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            8537..9004
                     /locus_tag="MBZ71_001842"
     CDS             8537..9004
                     /locus_tag="MBZ71_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBT4429795.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(9267..9749)
                     /locus_tag="MBZ71_001843"
     CDS             complement(9267..9749)
                     /locus_tag="MBZ71_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBT4429796.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(9849..10712)
                     /locus_tag="MBZ71_001844"
     CDS             complement(9849..10712)
                     /locus_tag="MBZ71_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBT4429797.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         3222 a         2268 c         2032 g         3529 t
ORIGIN      
        1 tagctcttct gcagtagaaa attttacacc gccaaaaatt tccgaaatcg ccttttgcaa
       61 gaatcccgtc gaatattaga aattggatca gtatgcttaa acagtaataa aaaagttatg
      121 acaagatgtt gtcacaacct tttttgagta aacggtgttc aaaaacttgc atctgcggta
      181 ataagttcaa ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt cttactactt
      241 gatgcagggc agctttttca ctacctcttt ttaataacca taaaatccta ttccttcata
      301 tctccagccc atcggtgtta gccaatctct ttccttagca ctcaaggtat agtgatgggt
      361 accagttttt tcatttggat taaatagtcg ataaattggc ttggaaccac cagaataaaa
      421 acttagacct tcataacgcc aaccgatttt tgttaaagag ttgatctcag attgtgccat
      481 tgtgtaatga tggtctccac tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt
      541 tggtgcttgc caagcaatcc cttcatattc ccagcctaca gttggcagat aatttttttc
      601 tccggcattc aatgtatata aatgttctcc tgtatgacgg ttatacagac gatacatcgg
      661 agaagttgaa gttggtgctg tatcatactg tgtcagatta taggtttcga ttacattgtt
      721 caatgtagaa ccataattag gcgcagtggc ataacgacct gttagccaag ccgtagcatc
      781 tttgtaagag ttcgtattgc ttttccatgc accggaatag tagtaaacgc ctggctgaaa
      841 agaagtcgtc ttcaataccg ttacgttatc ttgcaatgat tctttgtacg aaggatattt
      901 tctaaaagcg gcattcaccg taatccattg cccattcaca tattccgaag taggcatcgt
      961 aacagattgg ccattatagc ttcctttaat cccaaataga ttgtaattag gggcagaagc
     1021 caaagtactg ttcccccagc cactttcaac gatggcttga gcaatcatga ctgatgcata
     1081 taagtcattg gcacttgcca aactttgtgc agttgaggca atactatcaa tgaacgcttg
     1141 ttgagaatta gcagatgagc gagcataaaa agcatagcca gtatagtttt cgaattcaga
     1201 caaatctgct tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc
     1261 ttctgcattc tctggattgt tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg
     1321 ttctgttgtt tcagttgttg gttcttcggt tgaaattgta ttttctgaga cagtagctga
     1381 ctgcaaagta ctctctgtat cgttttcaat tcctgtttct gaagaactcg atgtctgagt
     1441 tgcgacagtt tctgtcgatt cttcagctag cactgttgtg ttggcaactg ctagatttcc
     1501 taaaagaata acgctaaata ataaattctt tgctttcatc ttttttctcc tttgaattat
     1561 gaaacttttc ctaaataaca gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt
     1621 aaataacatt tgtttctttt tgataaattt gctttttcta cataaaaaga cgtaaatgaa
     1681 gaatattctt ctccattcac gccttaaatt gatggtcgtt tgaataacta tttaacaagt
     1741 aattctcttt ggatattatc cgttatggat tatacttcct gtgtcgtcgg acggataatc
     1801 atgttactga cagacatccg gtctggcgtt tctatcgcaa atgctacagc agaagctata
     1861 tcttcggagg tcaatcccca ttctttttgc gcttcatgaa gttcaagagc agcctttttg
     1921 tcgctgatgg tttgatataa ttcagtttga acagctcctg gagagatgat cgtagatttg
     1981 atgttatttt gccgttgctc ttgacgtaat ccttccatga ttgcacggac agcaaatttt
     2041 gttccgcaat aaacggcgga atcaggataa acaacatgtc ccgccacaga atcagttgaa
     2101 atgatatgcc cagatttttg tttttccatg attggaagta cggcggaaat tccatttaac
     2161 acacccatga tgttgatatc aagcatattt tgccattcat cacggtgacc ttcaactaat
     2221 ggagcggttg gcatgattcc tgcattattg tacaatacat cgattcgacc gaacttttcc
     2281 atcgctaaat cgataactgc ttggacttgt gcaaaatctc ttacatctgc tgtttgaata
     2341 tatagctcag cttctggtaa agattctttt attgctttta ggcgatcttc tcggcgtgca
     2401 gctataacta gtttagcccc tttttctgct aatagcttcg ttgttgcttc accgattccg
     2461 cttgaagcgc ccatgatgac aatcactttt tctgataaat cattcattct attctcctcc
     2521 tgttttgaaa aatctcttgc ttatactcac tatctcttat ttcatcttac tgttcaaata
     2581 atccgttcga caggtttgac gattttggca gggattcctg caacgatggt attgtctggg
     2641 acatctttcg taacagttgc attcgctgca acaatggcat tctcgccaat cgttacgcct
     2701 ggcagaatag ttgctcctgc tccgatccaa gcatttttct tgatgtggat tggctttact
     2761 gacaagcctc ttcgttcttt tggatcggtt agatggttca ccgtgatgag gttgacacga
     2821 ggaccaatca atactgaatc ttctaaacag atgccaccta agtcaacgaa catcacttga
     2881 cgattgataa agacattttt ccctatacta atatgtttgc cgaaatctgt ataaaaaggc
     2941 agcagaacag tcaccgtatc atctactttg tcactaatga tattttctag ccgtttgcgg
     3001 atatctgctg gttcatggta cccagtattc agcgctgcta aaggctcttc gttttcagct
     3061 tggatctgat ggatcacttc aaagagcgga tccgttactc gaatctcttt attgacgatt
     3121 tgtttttcga tgaggcttgt cattttgctc cctcctttta tccttacttt ataacggacg
     3181 gcttatcatg tctaatactt ataaaacata cttaactatt ccttttaact atacttagtg
     3241 gtatgctttt ttaaaaacag gagggaaaaa atggatattc gtatactgac gtatttttta
     3301 gcagtagcta gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat cacgcaacct
     3361 actctctcaa aacaactgaa agatttagaa gaagaacttg gtgtgacact tttcactcgg
     3421 ggctcccgag agatacagtt gacggattct ggtcgatatc tttattcaaa aggaaaagaa
     3481 atcttgtcgt tggtagaaaa aacggcgaac aatttaacaa atgacgcaat catctctgga
     3541 gaaatctaca ttggcggagg cgaaactaaa gcaatctctt tcatttctga tagccttcat
     3601 tcgttgatca aggcgcatcc tgatatccag tttcatttat atagcgggaa tgcagatgat
     3661 atcatagaca aactggatag cggattactt gattttggac tcgtaatcga accgacaaac
     3721 aaacaaaaat accagtcatt tcgactgcca gcccaagatc gttggggact tttgatacat
     3781 cgaaatcatc ctttagcaaa gcaaaccgtc atccatccgc aggaactgaa aactgttcct
     3841 ttgatcattt cccggcaaac gtcagtggac agtcaactag ctgaatggct aggtttcagc
     3901 atagatcagt tatcgatcgt cggaacatat aacttattgt acaatgcttc tttgatggtc
     3961 gataacggga cttttggtgc attagctcta gatgggatca tcaatactta tgggacggaa
     4021 aaaatattca tcccactcga tccgccatta caagcttcac tgaatctcat ttggaaaaaa
     4081 gaccagcctc tttctcctgc tgctcgtaaa tttttagatg tcctaacgga gaaatacagt
     4141 taagtagagt ttttttcctt cgctttcagc tttttaacaa cgaataagat aagcaaaaga
     4201 tccaacgatt aaataacata agtttcttaa tttatttctt ccttttattt ctgataattc
     4261 cagtaaatta ctataattat aaaaaaagaa ttgacaaaac ataactgtct atataaaatt
     4321 gattacgtta acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct
     4381 gaggaacatg gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact
     4441 ggtctagcaa acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga
     4501 tctttaatca aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa
     4561 aagggattgg atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga
     4621 tatttgtcat ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg
     4681 aacgcttttt atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa
     4741 agttgaatga acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca
     4801 ttaataacgg tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg
     4861 atgaattcag tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat
     4921 atacttatga cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga
     4981 acaataacca tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg
     5041 aatcagaatt ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg
     5101 tgaatcattt acgactgctt ctacaacgaa cccatgcgca acaatatgct gttttagaca
     5161 cggaaatcgt agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa
     5221 tccgtgtgtt gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact
     5281 tggccatcca tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg
     5341 aggaggaaaa aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt
     5401 tgcggtctta cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag
     5461 taacgacatt gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc
     5521 tttgctgttt gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc
     5581 cttggcaggt ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc
     5641 tactttattg aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt
     5701 atttattggt attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc
     5761 aaaattaccg atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc
     5821 ctttgtcatg ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc
     5881 tttagtttct tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg
     5941 cttcttcaac cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg
     6001 gtttgacgta gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg
     6061 cgtaacagga atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg
     6121 agcatatgct atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat
     6181 gctagcagca gcttttgctt cattctttac tggtgtgaca gaaccacttg aattttcatt
     6241 tatgttcgtt gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc
     6301 agtgagtgca ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta
     6361 cttcctatct ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt
     6421 agtaacagca gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat
     6481 gactcctggc cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga
     6541 caataaatat gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt
     6601 aacttcgatc gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat
     6661 cgatcaaaat aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa
     6721 tgcgcaagtc atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca
     6781 tggtggtgct gcagcacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc
     6841 cggtgaaacg atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac
     6901 tgaagtttca gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc
     6961 agaaaatgga gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa
     7021 acatgctgtc ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa
     7081 cactgttgac ttaaaaggcg aaccattcac tttatatgta gaagaaggtc aaaaagtcgc
     7141 aagaggtcaa ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac
     7201 ggatatggtc gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc
     7261 acgtgatgta aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt
     7321 cttccccaga cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc
     7381 tcctttttcc gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagctaa
     7441 acgaaacata tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc
     7501 tttttcacaa aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata
     7561 cattattcct gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc
     7621 aatacttttt ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg
     7681 atcgctaaaa acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca
     7741 aacgctgcat cacttgcttc aataatttct aactctttac ctgtcgcacg ctctaatgcc
     7801 ttggctaatt cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca
     7861 ctttctacta gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt
     7921 tttccatttc ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt
     7981 aattcattct ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta
     8041 atcatttttt cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct
     8101 gcaaaacttg tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc
     8161 ttatgttcgg cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct
     8221 ttcaacgctt ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca
     8281 gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt
     8341 ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt
     8401 actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta
     8461 catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat
     8521 gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc
     8581 ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat
     8641 cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat
     8701 ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt
     8761 actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac
     8821 aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg
     8881 gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa
     8941 aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt
     9001 ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta
     9061 ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag
     9121 cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc
     9181 tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa
     9241 gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct
     9301 cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag
     9361 gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt
     9421 aattcttcag gcgattctgc taaagcgctt acaacaagat acgttccttt tgggaatggg
     9481 atgattcgcg tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt
     9541 ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat
     9601 gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa
     9661 ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct
     9721 ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca
     9781 tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta
     9841 ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa
     9901 tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaacccata atctgaagaa
     9961 ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct
    10021 ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac
    10081 ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag
    10141 cggtctccat caaatccata agaaacgttg attacttgat ttggatataa atctcctaac
    10201 ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga
    10261 ttgactcctt caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct
    10321 ttcttttcga tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag
    10381 cgtggaaaag atttttgtat gccgtatcta ctgatttctg aaggcagata tcctgaccaa
    10441 tcacgaaaag ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct
    10501 gtcactcttg atccatctag aagatcgcga ttagccaaag ccaatctacg gtttttgata
    10561 tagtccatta gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag
    10621 attcctgtat tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg
    10681 taatcgatca attggttcag atctttaagc atatttatct tcctccttga ttgccttttt
    10741 atcatattat caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta
    10801 gaaaatgttg tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt
    10861 attatctgcc tatctatgtt ttacctttag aaaaataaat tttggggaag acagattcta
    10921 agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg
    10981 tacaaagcaa attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga
    11041 tacatcatac c
//
LOCUS       DAGCWU010000094        11041 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.094, whole genome shotgun sequence.
ACCESSION   DAGCWU010000094 DAGCWU010000000
VERSION     DAGCWU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.094"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..222
                     /locus_tag="MBZ71_001845"
     CDS             <1..222
                     /locus_tag="MBZ71_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429798.1"
                     /translation="LTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVEGKIKSNQTK
                     KYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            241..1833
                     /locus_tag="MBZ71_001846"
     CDS             241..1833
                     /locus_tag="MBZ71_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429799.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            1845..2180
                     /locus_tag="MBZ71_001847"
     CDS             1845..2180
                     /locus_tag="MBZ71_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429800.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2327..2521
                     /locus_tag="MBZ71_001848"
     CDS             2327..2521
                     /locus_tag="MBZ71_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429801.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            2553..4925
                     /locus_tag="MBZ71_001849"
     CDS             2553..4925
                     /locus_tag="MBZ71_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429802.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            4986..6446
                     /locus_tag="MBZ71_001850"
     CDS             4986..6446
                     /locus_tag="MBZ71_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBT4429803.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            6457..8661
                     /locus_tag="MBZ71_001851"
     CDS             6457..8661
                     /locus_tag="MBZ71_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBT4429804.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            8682..8831
                     /locus_tag="MBZ71_001852"
     CDS             8682..8831
                     /locus_tag="MBZ71_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429805.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            8907..9500
                     /locus_tag="MBZ71_001853"
     CDS             8907..9500
                     /locus_tag="MBZ71_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBT4429806.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            9516..10415
                     /locus_tag="MBZ71_001854"
     CDS             9516..10415
                     /locus_tag="MBZ71_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBT4429807.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            10418..10903
                     /gene="ssb"
                     /locus_tag="MBZ71_001855"
     CDS             10418..10903
                     /gene="ssb"
                     /locus_tag="MBZ71_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4429808.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT         4228 a         1831 c         2292 g         2690 t
ORIGIN      
        1 ttaaccgaaa tggtttcaga gtttgcggat cttcatttga aagatacagg aatactaatt
       61 ggtacaaatt caactgttta tcaagaagca gaagaacgtg tggaaggaaa aattaaatcc
      121 aatcaaacca aaaaatattc ttctaagtta aagaaaccag tgaatgaatc tgttcatacc
      181 agagtagatc catttgatcc aacgaagatt ctaaaaagat aaaagtagaa ggagaacgtg
      241 atgaataaat cgcctgcagt tattcttact tgtcaattta ccttgcccaa taaaaaatct
      301 ttttcaacat acattgatta tatgacaaga gaaaaagcac tggaagaaat ggaacgacgt
      361 acgcccgaag aagaacagga actgcaacga atcaagaatg ctttagagga tttttatatt
      421 cctgaaggag aaactttttc ggaaagtaag acggataaag aattcagtga aacgtcgaat
      481 gaagctcaat cgttgatgga acatggaatc aattttgaca agttagagga acaagatttt
      541 accaaatatc tttcttatat gacccgtcac tatgcgttag aacaaaagaa agaactgacg
      601 caaagggaac aaaaagaata ccaacggttg aaccaagcaa tgaaaaaata tcaaacagag
      661 ccacataaaa aagcttcagg aaatcttcct ggagtttttt ctatggcttc agacgaagtg
      721 aaaggaagtg atctgaagga gatcaaacaa atcttccaaa gaggacaaca aaaaggctca
      781 atcatttatc aagatgttat ctctcacgac aattcgtatc ttgaaaaaat gggcttgtac
      841 gatcccaaaa cagatacctt agatgaagaa ggtttgaaag cagctggaag aaaaatgatg
      901 gagacattat tcgagaaaga acaattgaat gaaacaggtt actggatggc aacgattcac
      961 cgtaacacta agcatatcca tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa
     1021 ccgtatactg agattgaaaa tggtgtgagt tatatcgtac cgaaggggaa gagaagacaa
     1081 gcaacattag acgcaatgaa aaccaattat gtacatgagt tagaacgttt ctcttatgaa
     1141 aaggatcgaa tgatcggtat ggagaaacga aatttattga cacgaaaaag tgatttgcgt
     1201 aacaccttaa caaaagtagt aaaagaacca gcacgttacg atcgacacgc catgaactta
     1261 ttaaatgaag tatataaaac cctacctgaa aaacgttcgg aatggaatta tggagatgaa
     1321 aatcgaacca agctttcacc atttacaagg ggaaaacttg atgagttaac caaatatgtt
     1381 ttaacaaaag aacctggata tcaagaatat atacagattt caaaagcatt gaaagaagaa
     1441 gccaaaagcc tttatgggga aagcaaaaga gaaacaaaga atgcagaaaa aaacgccctc
     1501 tttgatttaa aaaagcgcac aggaaatgcg attttaacag aactgaagag acaggatagt
     1561 caaataaaac cattaataga aagtgtcaat cagatgcaat ctatttacaa tgaacgatcc
     1621 tttcgaacac tcaaacagtt gccaacaaat gaatacaagt atagttatca agaattcgta
     1681 aagcgtagga aacaattcca acaaagagtt gtcacgcagc gaagcttgcg tcaattaaat
     1741 cgaacaatta atgcacatga agaaaaatac cacgcaagta aagcatatga agagcaacag
     1801 cgaagaatcc aaatggaaca agaaagacaa taggaggaag agaaatgaaa caatatttag
     1861 atcaatggaa agtcatagaa ggatcactaa aagaagaacg aatcgaacaa cttcctgatt
     1921 gtttagaaaa agagcatttg tttcagataa gcgaaatgtt gagaaacgaa cagtttgatc
     1981 caaatcaatt tttagttgtt gagtacccag ctactggtgt gtattgttgt aatcatgtga
     2041 agggtgaaaa atattttatt attcaagaat acgaaggtaa gttagcgcct tattatacta
     2101 cttgggaaat aaatgaagag ggaatcaata atttcccatg tgaaagcatt gaagagtcta
     2161 tttctctcac ggaatgttaa ataaatagag acgtaaaaga gtcctgaacg tgattgtcca
     2221 ggactctttt tcaatgaatt gttttccgcc aattcactga gtggtgcgat tcaattttat
     2281 tatatcaaaa gaaagtgaga ttccaaagtg aagggaggaa gaagaaatga ctcagacaac
     2341 gattggtgtt gtggttgaaa aaagcaggga taatctcgtt tttgtgacag aaattcaaac
     2401 aggtagagct tttgtagtca cagacaaagc tgcaaaagcg tatcaaaatg gcaatatttt
     2461 aacgttaaat ctgactacga agatgttcgt tgatgcagta gaaaattatc catttgttta
     2521 gacaaacgat attttacaag gaggaaacgg acatggcaga agaaaaagaa ccactagata
     2581 atagtcgttt gggaaagtcc aaaagaaaat tagtacggtt gcaaaacgaa ttaaataatc
     2641 aatttcaaaa gctatttgat caccaaaaat tgacaaatgg gcaaccaatg aatgacaaac
     2701 gaaatggtcg ttcatggttt aaccagcaag aaaaaataga aaataaaata caaagtttga
     2761 gagaagagat caagcaacaa gaaaaacagg tagaaaaact tgaaagacaa gaagagctaa
     2821 aggaaatggg ttataacaag tacggtggcc tggacatgac gatcgaaaat attccaagaa
     2881 tcaaagaaga aatcgaacgc tttgaaaaag gtgaatcgac tttttcagct gccacgatcc
     2941 gaaaatacca acgaaaattg gaaacattag aacagctgaa agaaaaatca gagaaaggaa
     3001 aagaaaatct tcttcctgaa gtacaagcga tcattgacag cggacgagtg acgcaatgga
     3061 aaaagaatcc aactatctat tttctgaaag gctatcgaaa agtcgcttta gaactcaatg
     3121 aaaacggggg atttgaggaa tctaaaaaat accaagccac gtcggaagaa gaaaaggcag
     3181 ctgttcgtga attacttgaa gaagctacac ctactgattc tgtgaaggag aaagaaaccg
     3241 atcatttcta tcgagtagaa ttcaacgaaa atgatccagc aaaagggttg aaaagttatg
     3301 gtggggagat cgtaacacta gaattgatcg atcaactcaa aacactggat aaccaatacg
     3361 ccgcagaagc aggctattat aaattctatt ttgaagaagt gaagaatgga gaagtagtcg
     3421 atcagatacg aatggatcta ggggatggcg tcaatagtaa tcaccccatc tatttagagt
     3481 tagctagatt gtgcgaacaa tcacctgaaa aggaacaaga acaaacagtc aaaaatgaac
     3541 ctatttcaaa agtacaaaac gagattaaag agacaaaaac aaaacaaaag ggttatcaaa
     3601 aacgaacggc agaagagatt aagcaagaag tcaaatcact ttcccaacag gcgcttgatg
     3661 cggtgaaaaa gcatactcat tcacctgaag acgtcaaaga acttttgaat tttatgtctc
     3721 gttttccaga acgatccttt agaaatcaat tattgatcga ggaacaattt ccaggcgcaa
     3781 cggcttgttt aggtcgtgcc gcgttgaaaa aagaaggaat ctatatcaaa aaaggagaac
     3841 aagggaacaa gatctttgtc agaaaaacgg tcaaaggatt ttatgagaaa ggacgtggct
     3901 ttgttcgaga aacagaggct acaccagaag ataaaaaacg aatcgaaagt ggtcaaatcg
     3961 aagtgatcaa aaaaccttac tacacgattg aaaaagtgtt tgatatcaca caaacacaat
     4021 tgaaacctga agactatccg aaaatcttcc cgaaccgcgt ctttgatttt caattggatc
     4081 aaacagggca gacagaactt caagttggga ttcaagcggt agccaaaaat attggaattc
     4141 aaattcgaga tatgacagaa agtgaagtct atcaaagaga attaggacaa gcacgtgggg
     4201 cgtacgttcg taacgaattt actggcgaag aagaaatcgt catgaatact cgaaatactc
     4261 ctacgcagca tttagccaca agtattcacg aattagccca tgctagaatg cacaaatttt
     4321 cagaattaga tactgcaaca aaagaactac aggcagaaat gacctcctat attgtgtgca
     4381 agcattttgg gatggacacc tcggagaaag ctattcctta tattgcgaca tggacgaaga
     4441 atagtcagac gttggatgat aaggaagctg cggaacgtgg gaaaatcatg aatgatgtca
     4501 gtcgtgtcgc aaatgaattc attcaaacca tttctaatga aatcaatcaa tacagagaaa
     4561 gagaaccaga gatccagcca gcagaagtga agcaggagca taaaaatact gtagaaacgg
     4621 cagaacaaaa gaatattgtg cccgttggct cactttggat cgacaagaaa gacgggaaag
     4681 tcctggaaga agacacgggc cgaacgttac atttgaagga tgcggcgttt attgaagatc
     4741 cgcagcataa tcaagtgatt ttaaaaggaa aaagttatgg aaaagagatc caaacggcgt
     4801 taccttcaga agaatttgcg ctaggaaatc cattccgatt ttcacttgag acagtgtcag
     4861 aagtggcaaa acgaaaaaat gaaatctatt atgaacaaca attgtcacaa aaagaacgcg
     4921 tataaaacaa gcaagaaaga ctatctgaaa aatggatagt cttttttctt ttgggaggtt
     4981 tgaaaatgac agagaaaaaa atgagtctca ttgatcgatg caagcaaatg gatattgtgg
     5041 attttgcacg aaacaatggt ttagcggtgg ccaataaagg acgagactat cgcttagaag
     5101 accatgattc atttgttttt gatcgaagaa aacaacgttt ttattggaac agtcaaaata
     5161 ttagtggcga cattattgaa ttagctaaac tttttttcat agacaaagag atccaagacc
     5221 ctaagcaaca atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac
     5281 gagtggaaaa tcttcatttt gagacagaaa agtacaaaga tcaccccgtt gattatcaac
     5341 cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactgccc gattggttga
     5401 ttgattatgc agaaaaagaa ggattgattg ccgaactaaa gccaaaacat gaacggcaaa
     5461 atttcttagt tcgagacgat cgtttggatc atgcggtcgc ctttttatgg aaagatccac
     5521 agacaaaaga gacagtaggg gcgagttatc aaggaacgtt cattgattat gaacgttttg
     5581 gtgagagagg cacatataag catatagata aaaattcgac agcgaatcat ggctttaacc
     5641 taaagattgg tgatcctaaa cagttaaaat tttttgagag tagtattgat ttacttagct
     5701 acgctgcact gaatcgtgat cagttaaacg atacgtggtt agtctctatg gaagggttaa
     5761 aacaccatgt gatcagtcat tattttggtg aggcagtatc tgaattgaga aagaaacaag
     5821 cctttcctca atccatcgag atttgtgtgg ataatgaccg tgcaggacat attttctacg
     5881 aaaaagaaca attaatgggc gcagttgatc catttacgaa tcagaaagta cgttgtgaac
     5941 gaggaatcgc taatgattgg caagtgccaa gagaatacaa agtcatttat gaagaagtcg
     6001 caaaagaaga aaaggtcaca ccagaagcta ttatggccat acacaagaca gaaaacaatc
     6061 tgcaattaac gaaccaactg gtttccgctc acaaagtcaa tgcgtctttt ggtcagcagc
     6121 tttctgttaa cgattcaata gaagtcatta gtttgaaaga tatttgtaga gaagtcgcaa
     6181 aagaattaaa agattgtgaa cgctcgaatg gtacgtatga ttttgatcgt ttttatcaag
     6241 gaaaagggga tattaattct cagattcttt tctcttataa agcagaacaa tactacaaag
     6301 gctataaaaa tcatgaacat gaatttgtac ctgaagtgaa aaaggattgg aacgatcaat
     6361 tgaagcatga gatccatcaa caggaaatta gaaagcaaaa acgtgcgatg ttgtttcaac
     6421 aaggcagaca acaagaaagg gagtgagatc gtcacgatga aaactgtcat tttagcagaa
     6481 aaaccgtcac aagcgaaggc ctatgccgat agcttttcta aagccacccg aaaagacggg
     6541 tattttgaaa tccaagatcg attgttttcg ggtgaaactg tgatcacgta tggttttggt
     6601 catttagtcg agttagattc gcccgatatg tacgatgaaa attggaaaca atggtcatta
     6661 gagcatttgc ctattttccc tacacattat cactatcatg ttcctaaaga taagaaaaag
     6721 caattcaacg tggtgaaaca gcaacttcaa tcggccgaca ccattattat tgcgacagat
     6781 agcgatcgtg aaggggaact gatcgcttgg acgatcattc aacaagcagg cgctgatcaa
     6841 ggaaaaacct ttaaaagact ttggatcaac tccttagaaa aagaagccat ttatcaggga
     6901 ttccagcaat tacgagacgc agaagagacc tatcccaaat ttgaagaagc acaagcacgc
     6961 cagatcgctg attggttgat cggcatgaac ggcagcccac tgtatagttt actcttgcag
     7021 caaaagggca taccaggaag tttttcctta ggcagagtcc aaaccccaac gctttacatg
     7081 atttatcagc tgcaggaaaa gattagaaac ttccaaaaag agccttattt tgaagggaaa
     7141 gcccaagtaa ccgctcaaaa tggcatattt gatgcgaagc ttgatcccaa tgaaacccaa
     7201 gcgacgcaag aagcatttga agcctattta aaagaaaaag gtgttcaagt aggaaagcag
     7261 ccaggcacga ttcttgaagt cgaaaccgag aaaaaaagcg cggcaagtcc acgtcttttt
     7321 tctctatcca gccttcagtc taaaatgaat cagctcatga aagctagtgc caaagatact
     7381 ttggaagcca tgcaagggtt gtatgaaggg aaatatctaa gctatccacg aacagacacc
     7441 ccttatatta cagaaggaga gcatgcgtat ctgttggatc atttagatga atacaagcat
     7501 tttttaaagg ctgaatcatt ccctactcct attcatacac cgaatagtcg gtatgtgaat
     7561 aataagaaag tccaggaaca ttacgcgatc attcctacaa aaacagttat gacagaagca
     7621 acgtatgaaa agctgacacc tttacaacaa gcgatttatg atcaagtttt aaagacgacg
     7681 gtggccatgt ttgcggaaaa atatacctat gaagaaacta cgattttgac acaagtccag
     7741 cagcttcagc taaaggcagt cggaaaagta ccagtagatc taggttggaa aaagctattt
     7801 ggcaaagaga gcgacagaaa agagaaggaa gaagaaccgc tacttcctaa agtaacaaaa
     7861 ggagaaacgg tgacggtaga tctacaaatc tttgaaaaag aaaccaagcc accacaacct
     7921 tacacggaag gtacactaat caccgccatg aagacagctg gtaaaaccgt ggatagtgaa
     7981 gaagcacaat ccatattgaa agaagtggag ggaattggta cagaagcgac acgagcaaat
     8041 attattgaaa ccttgaagca aaaggaatat atcaaggtag agaaaaataa acttgtggtg
     8101 acgaataaag gaattttgct ttgtcaggct gttgaaaaag aaccgctgtt aacaagtgcc
     8161 gaaatgaccg ccaaatggga aagctatctt acaaagattg gtgagcaaaa agggacacaa
     8221 gctacttttc tagcaaatat ccaaaaattt gtttcccact tgttggaagt cgtcccagga
     8281 caaatccaag ccactgattt tggagcaacg ttacaagagg tgaaagtagc cagtgaaaag
     8341 caagaagcgg cgcgtcattt aggatcttgt ccaaagtgcc aggaaaaaga agtcatactt
     8401 tatcagaagg tggcgacttg cacgtctgaa gcctgtgatt ttaaattgtg ggtaacgatt
     8461 gccaaaaaga aactcacagc gacacaactg aaagagatca ttcagaacgg ccgaacaagt
     8521 caaccagtca gaggactaaa aggtcagaaa ggttcgtttg aagcaacgat cgttttaaaa
     8581 gaagacttta caacaggatt tgagttttct gaaaagaaga agaccaccta caaaagaaaa
     8641 gtgagacgta aaacgagtta gaattgacgg agggaaaaac gatgagaaat acaacgcata
     8701 aagaacaaga aatcgaaaat caaaaagcca aaatacgttc agagcaggag aaaatgcgtg
     8761 ccagcaaacg aagactacag caattgaaga atcaacaacg gattcaaaaa gaacttgaat
     8821 tagatcgata aatcctcctg ttgttaatat taaactaaca tgttatagtt atgttagttt
     8881 aatattaaca ttcgagaggg aaagcgatga aagataaagt caagtgcatt tttgagaaat
     8941 acaatggtaa tctatccttg aaggatgcta gaaatgaagg gttatcacct atgacgttag
     9001 atcgtttagc cgaaaaggac aaaatagaaa aaatagcatc aggtttttat accttaaaag
     9061 atagttttgt ggatgaatta tatctcgctc aaagtatcta tactagagga atcttctctc
     9121 acgagacagc gcttgatctt tttcaagtag gaacatatat ccctaaaaaa attaatatga
     9181 tgtttcctaa aggatacaat gtttcgaaag agaatttaga tcagtatgcc attcaaccgc
     9241 attacacgaa taaagaaaac tttgaactgg gaatcacaga ggcagaaagc ttttacggca
     9301 ataaaatttt cgtctatgat ttagagcgaa cgttatgtga tatgtggaat cccagatata
     9361 aagcaagcgt agaagtaaaa caagaagcat taaaagaata tatgacaagt accagcagaa
     9421 atacaaataa gctaagaaga tacatgaata ttttaaaatc accaaaagaa atgactttgt
     9481 atatgttgcc actttattga gaggagtaaa ggtgaatgac accgacacaa tttaaagcga
     9541 aaacaagaaa tgttgcagct gagaaacatt taaacccaca aattgttcaa agatattata
     9601 tgatggaaaa attattagaa tgtattgttg aatcgcctta tcatgatgat tttgtattaa
     9661 aagggggatt tctgatcgga tcaaaatacg ggttagaaaa ccgttcaacc gttgatattg
     9721 atacgactta tcgaaattcg aaactaacaa aagaaaaatt agaaactgtt ttttctgaat
     9781 taacagctgc tcctacaaaa gatggcattc ggtttgaatt aaagggctta acagagacga
     9841 gggaagccga ttattaccca ggttttcaag caaagtttat tgcttttctt gaaaatgcac
     9901 gtgtaccttt taaattagat attacttcag gtgattctat tattccagca gcattgatat
     9961 atgaacataa attgatgttt gaagataaaa caattcatat tccagcttat cctacggaac
    10021 aaattttagc tgaaaaatta catgccacgt ttagtttcgg aatagataat tcacgtatga
    10081 aagatttcta tgatctgtat atgatcccta aactagaacg tatcgatgag ataaatttgt
    10141 ataagagtgt tcaaggaaca tttaaggaaa gaaataatca atcagtgttt aagaatttgt
    10201 ataatcagga aatgcctcaa ttgatgaaga atgagggatt gagggagaaa tggaaaagat
    10261 atcagcaaga aaactatttt gccaaagata tttctttcga atctacctta acttctgttg
    10321 attcgctgat gcaatcaatt gtcgagcaag aggaagtaga acgaaaacaa aattttaaga
    10381 gatcacaaca aatgaatcaa gaaatggaga gataaagatg atcaataatg taactttagt
    10441 aggtcgatta accaaagatc ccgatttacg ttacaccgca agtggcactg cagtgaccac
    10501 ttttacctta gccgttaacc gtaattttac taatcaaagt ggcaatcgag aagctgactt
    10561 tatcaattgt gtgatttggc gcaaaccagc tgaaacgata gcgacattag ctaaaaaagg
    10621 cagcttgatc ggcgtcgtcg gacggattca aacgcggact tatgacaacc agcaaggaca
    10681 gcgtgtctat gtgactgaag ttgtggccga taatttccaa ctattagaaa gtaaagcagc
    10741 gacagaaagc cgtatgcacg ctgatcaatc gtccagcagt ccaagcccta ccacctttga
    10801 acaacgggat acagcgacgc ctaacaacaa tggattgaac gcttcacaga atccatttgg
    10861 tggacaatca atcgatatta gcgatgatga tctaccgttc taagtagcat tttctattgg
    10921 ttgcatttgc tacggtttat gttataattt agacaacaaa aagaattttc aatctataac
    10981 caaaaaccac tagctggcgg gctagtggct ttcttttacg tttctattat aagaattaac
    11041 g
//
LOCUS       DAGCWU010000095        11000 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.095, whole genome shotgun sequence.
ACCESSION   DAGCWU010000095 DAGCWU010000000
VERSION     DAGCWU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.095"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(63..1268)
                     /locus_tag="MBZ71_001856"
     CDS             complement(63..1268)
                     /locus_tag="MBZ71_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4429809.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="MBZ71_001857"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="MBZ71_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="MBZ71_001858"
     CDS             complement(1447..2832)
                     /locus_tag="MBZ71_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBT4429810.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="MBZ71_001859"
     CDS             complement(2861..3247)
                     /locus_tag="MBZ71_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4429811.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="MBZ71_001860"
     CDS             complement(3263..3577)
                     /locus_tag="MBZ71_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4429812.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="MBZ71_001861"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="MBZ71_001861"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBT4429813.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="MBZ71_001862"
     CDS             4837..6126
                     /locus_tag="MBZ71_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBT4429814.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="MBZ71_001863"
     CDS             complement(6165..6770)
                     /locus_tag="MBZ71_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBT4429815.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="MBZ71_001864"
     CDS             complement(6966..7430)
                     /locus_tag="MBZ71_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4429816.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="MBZ71_001865"
     CDS             complement(7452..7772)
                     /locus_tag="MBZ71_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBT4429817.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="MBZ71_001866"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="MBZ71_001866"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBT4429818.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="MBZ71_001867"
     CDS             9194..9544
                     /locus_tag="MBZ71_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBT4429819.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="MBZ71_001868"
     CDS             9729..10682
                     /locus_tag="MBZ71_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4429820.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..>11000
                     /locus_tag="MBZ71_001869"
     CDS             10695..>11000
                     /locus_tag="MBZ71_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429821.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     "
BASE COUNT         3162 a         2203 c         2051 g         3584 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt
//
LOCUS       DAGCWU010000096        10891 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.096, whole genome shotgun sequence.
ACCESSION   DAGCWU010000096 DAGCWU010000000
VERSION     DAGCWU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.096"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            148..1371
                     /locus_tag="MBZ71_001870"
     CDS             148..1371
                     /locus_tag="MBZ71_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBT4429822.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            1406..2173
                     /locus_tag="MBZ71_001871"
     CDS             1406..2173
                     /locus_tag="MBZ71_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4429823.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            2363..2761
                     /locus_tag="MBZ71_001872"
     CDS             2363..2761
                     /locus_tag="MBZ71_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBT4429824.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(3067..3441)
                     /locus_tag="MBZ71_001873"
     CDS             complement(3067..3441)
                     /locus_tag="MBZ71_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429825.1"
                     /translation="MKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLWKYKILFL
                     FWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(3463..3840)
                     /locus_tag="MBZ71_001874"
     CDS             complement(3463..3840)
                     /locus_tag="MBZ71_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429826.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(3830..4225)
                     /locus_tag="MBZ71_001875"
     CDS             complement(3830..4225)
                     /locus_tag="MBZ71_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBT4429827.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEDLLEK"
     gene            complement(4292..4477)
                     /locus_tag="MBZ71_001876"
     CDS             complement(4292..4477)
                     /locus_tag="MBZ71_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBT4429828.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(4559..4813)
                     /locus_tag="MBZ71_001877"
     CDS             complement(4559..4813)
                     /locus_tag="MBZ71_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429829.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(5087..5371)
                     /locus_tag="MBZ71_001878"
     CDS             complement(5087..5371)
                     /locus_tag="MBZ71_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429830.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(5865..6404)
                     /locus_tag="MBZ71_001879"
     CDS             complement(5865..6404)
                     /locus_tag="MBZ71_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429831.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(6517..6783)
                     /locus_tag="MBZ71_001880"
     CDS             complement(6517..6783)
                     /locus_tag="MBZ71_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBT4429832.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(6785..7006)
                     /locus_tag="MBZ71_001881"
     CDS             complement(6785..7006)
                     /locus_tag="MBZ71_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBT4429833.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            7232..8050
                     /locus_tag="MBZ71_001882"
     CDS             7232..8050
                     /locus_tag="MBZ71_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4429834.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            8054..8350
                     /locus_tag="MBZ71_001883"
     CDS             8054..8350
                     /locus_tag="MBZ71_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429835.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(8532..8885)
                     /locus_tag="MBZ71_001884"
     CDS             complement(8532..8885)
                     /locus_tag="MBZ71_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429836.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(8905..9960)
                     /locus_tag="MBZ71_001885"
     CDS             complement(8905..9960)
                     /locus_tag="MBZ71_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBT4429837.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            10176..10574
                     /locus_tag="MBZ71_001886"
     CDS             10176..10574
                     /locus_tag="MBZ71_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429838.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            10577..10750
                     /locus_tag="MBZ71_001887"
     CDS             10577..10750
                     /locus_tag="MBZ71_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429839.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         3615 a         1697 c         1872 g         3707 t
ORIGIN      
        1 aaatgttcta aaatagcgat ttctgctgta ttttcaatga aatttgattg gaaaaaataa
       61 aatgtgtttt actgacattg agttatgtta taaatttacg atacatttct tattgtaacg
      121 tatcgaaaac aggtctatac ttattcaatg gaatcgtacc agttccaagt ttttaagctc
      181 gtaccaagct tagaaagaag aggcaatcgt atgaaaataa cagaaaagaa tgtaaacccg
      241 aaatttgaaa ctaccgaaag caatctaaaa aagtttgatt taactgttac tttatcgaat
      301 caacttgtag atagcattgt tgaaatggat gctatgccac aaaaaattta tctttacgct
      361 ataggacatc ttgaccctaa aaatccgcct tttaataata ccgtcaaagt atctaaaaag
      421 gctttatttg cgttgatgtc ttctagttca tcttcttcaa aacattcaag attaaaacaa
      481 gttattgaaa aattgcatca gcaatctgtc attaaaattc aaagaactaa acctaacggt
      541 aagactgaat acaaagtcat ctcaccgcta gaaattactg aattcaatga tttttctgat
      601 gcagtatcta ttaaatttag tagttcaatt atgcctttat taacgaactt agaagatcat
      661 tttacgcaat atacatttga tcaagtagtt aacttgacaa gcaagtatgc gatcagtttt
      721 tttcaattat tcactaaaga ttttaatctg tatgaagcaa atagagataa cttaacatgg
      781 agtacaaaat cacttgatag attaaaaaat cctgttttct ccattgcaga tttacgaaag
      841 aaatttagta ttgagaaaaa atataaagct tttcgagact ttgaaagacg tgtaattaaa
      901 gaaccgattg cccaactgaa taaatacact gattttaaaa ttgactatga gcttatcaag
      961 aagggtcata cttatacaaa cataaagttc ttgcttacac gtaccatgtc agttccggta
     1021 ccttataagc aagaacaaag agaccctgct tatttaaaag gcgcagaaga taaaacagag
     1081 aaaatgaatc aatcgtactt gaaagctatg aatagcaaat acactaaatt actaatcaaa
     1141 aactttttga tcagttctgc cgacttatgc gatcttaaac ttatgagccg acttcagaat
     1201 aaagtttatc cgctttatga tcgtttatcc aaagatagag gatttgaatc agttgtcaat
     1261 cacctatctt acgttgctac tcataaggag gattacacaa taaaaaatac tgttaaatat
     1321 ctcgaaaaat caattacgag ttatttgcct tatgtgaacc tttatgatta acaactgaaa
     1381 aaatgcgtga aggaaggaaa aacttttgaa aaaagatgaa ctgacaaagt acaatgagga
     1441 gttcttagca acaattgata tgaaaatgga tttaaagtcg tatgaacttt atcaattgct
     1501 gttcagttat atctcaccca caaagctaaa gaaaacaggc gtagtagatt tacctgttaa
     1561 agcgattatt aattacatgg atgttgatca aaatcttgaa acggaactaa aaaccatagt
     1621 taacactttg caaagacaag cagtttatga aatatcgaat ctagacaaga aatataagac
     1681 gtatactact gtatctgcta ttgatcggat tcattgggat ttagaagagc atagtgtatc
     1741 tttcagtttt tcagaagatg taattccctt aattgtctca atcaaaaaga agtatccgtt
     1801 ctttgttttt aaagacttag catcttttca aaaaaaatat agcatcgtgt tatacaaata
     1861 tttggcgatg tattataaaa aatatcttat cgccaaaaat aaaggagatt ttgagaaagc
     1921 tgaaatatat aagaatcccc tgattgaaat agttaaaatt cgtgagatta cgaaaacaga
     1981 aaatgagtat aaaacaatat cgaattttga tagacgtgtt ttaagggaat cagttgaaga
     2041 aattaatgaa aaatcaatgc tgaagattga ttttgctaaa gttagaaaag gcagaaatta
     2101 caattttgct gtccagttta atatttctag tgagtttgat gaaaaacctt atagagaaaa
     2161 aaaattaggt taatgcaaaa tatatcttac tcgtttgaag atgcttcaga cgtatgttaa
     2221 gatatgtagt gccttgggag caaggtatta atctccttta gaggttaatt agcttgtgat
     2281 cgcctatcaa ttttttggta ggcgattctt tttttgaaac caaaaagcaa aaattatttt
     2341 ttaattagag aggtgcaatg cgatgaaata ctttttttgt gcagtattcg aatggaaagg
     2401 aaatgaatat gcagttaaat tcccagattt tccagactta tatacgcatg gtgaaacttt
     2461 agaagatgct tttgagatgg cgcaagaagt attggaagga tacttggtgc ttttagagtt
     2521 gcaagaaaaa gaaattccat cgcctacgcc tgttcaagag ttaaatgcaa gaatcaagcg
     2581 atctgagaat gttaatttat ttgtcgtaaa agctaatacg aaagttgcta aagctcgaaa
     2641 caagcaaaat tatgatcgaa aatcagtgac tgtggaacac tatttaaatg ttttagcggc
     2701 tgaagagaat attaatctgt cgaaagcttt gaatgatttg ttgagagaaa agttgatttg
     2761 atgtctctca gatgatcata ttaagcaaaa acagcttgtt ttaggacgta tggatttaag
     2821 aaagacaaat tatcaaagaa tatattaaaa gctctcagaa gcgttctgag agcttttaaa
     2881 ttgctatgta ttatactgca tctgctcaaa ttgattttct tttcatgctt accatgtatc
     2941 tagttttcca tcaagtaatt tttagatatg tattgaggaa taaatagcaa atttgcttga
     3001 tataagctcg aattaggtgc tttgtaatgt gttaggataa attatacaag aaaatgtcaa
     3061 aagctctcag aacgcttctg agagcttttt atcggtaggc ttgtttaacg tgatcttgaa
     3121 agataccctt ttgatgtttc tctcgatccc tgcatcgcta attactaagt gttcttcaag
     3181 tgtgatgtag gtatataaat taagcatcaa aatggtgtag ataagcaaaa aactccctaa
     3241 aactaagtgt tgattataaa aatctgactt ataaaaaaga actgagaatg ttagtgttgc
     3301 ttcccaaaaa agaaacaaaa ttttgtattt ccaaagatag tggatcaagc gctgtgcttt
     3361 actcatctca aataatttat aataacctgc taattgatca gcaagcgtat cgttaatggg
     3421 ttctgtttgt gtttgtttca tctttctctt ctcctgtttc aattaatcta aaatttcaga
     3481 ttgagcaagc aactcattgt ggtagtcggt ttgaaattca aataacaatt tagccttctt
     3541 aacgaggttt aaagcgttcg ttagtgattc acttaatgct tcaacatctt taactgattt
     3601 tgcatgtttc attttttcag tgtgcgtata gcagaattca ggtattgagt taggagttgc
     3661 ctgttcaata aacatctcaa ttcgcaagtt attatcctca aaaataatta gatcattggc
     3721 aaagtgtttg gtactaacgc taatgccagt tgtatctttt aatagattat tgattaacgt
     3781 tcgagagaaa tagtttaagt gtgtagattt cataggttcg gtctcctttt tacttttcaa
     3841 gcaagtcttc ttctagtgct ttttgaagta attgagagaa gtttatgttt ttctccttcc
     3901 ctaaaagaac taaccattcc ggaacactaa cattttttct aattgttttt gtatttaagc
     3961 ttcttcggaa aagtttaatg tttagttgga tgattactac tacttgattg gctttcaaag
     4021 ttaattcttc cactgctgat ggtttaggaa attctgcatc tggtacacta tatagcgcta
     4081 atcctaaagc atccttagcg tttaaatatg cttcttcaag actatttcca aaggtagata
     4141 tttccggtaa gtcagggaaa acgatgttat aagcttctcc ttctttctcg aagattgccg
     4201 gataaaaaat attttttgtc ttcatttcct cgctcctttt ctagtgtaag agaggatagt
     4261 atgcctaata ccaaatagga aggcaaggct gttaaagccc tgcttgcttc cagatattag
     4321 cttctgttcc tagtggtaca tcaccattgt ggatcggaac aacgaccgtt cttttagtct
     4381 tggaattata aaattttgca tgactgccag tttgtccagt ctttttgaag ccgttagatt
     4441 tgagaatttt tacaatttct cttgctgtct taggcatagc taccctccct ttacaaaatc
     4501 tattatacac attgttgtgt atggaatcaa tagaaaaaga ggatgtttta ttgcaaaact
     4561 aataaaatga tttattggtt ttttcagcaa attgaatgtt attagcgttg caaatattca
     4621 taatttccca agtatcatta gaagtatagg tatctaagaa ttcattgaca gttacggttg
     4681 attcgtatgg ttcataagtc aaagcgttct ccaagtcttt tgatgaaatg atcaagactt
     4741 tttcaatttc ctcccctttt tcatttgtga aaacgtattc caaagtgatt tgttcttcat
     4801 cggtataatt catattgtgt tctcctttct tgtattaaga aaaatggata tttccaattg
     4861 catcccactc ggcaccattg tggtataaga cgtgaccgag cgttacgcct ttgaaatgct
     4921 cttctaaata catctaaacg cattcaaggc tagtacctgc tttgagattg tagaagtctg
     4981 catccaatac cttttcagaa gttatcggat caatgcgagt tggtacctca ataaattcag
     5041 tccaaatttc tgcaaaagtc ttaccttcca aagtccacac tccctatcaa ttaattccaa
     5101 aagcaaatag agaaatcaag cgaccgtcaa tagtcacttc aacaactgaa aatgccgttg
     5161 aaacagtttt aacatccaca ctttccaatt tccctatgaa ggaatttttg tgctttagaa
     5221 tagctttctt taactgtctg ttatacgctt gacgactgtt aaactttttg aattctcgat
     5281 ccttgatcag ctgtttaacg acccctgcat taattgtata gctttctgca atagcaacgt
     5341 gagtaggttg ctgtaatgta gctgttagca tattgtcact ccttcctaaa agttaattag
     5401 cttgtttaca attacattat acacactagt gtgtataatg taaagagagg ttttttgctg
     5461 atggatcagc tgtgctaaac aaaagaaaac gatagagtaa caaaaaaagt cagcaaagtt
     5521 aaaaaggagg aaacggctag tccggttgct ttgcttgtgt aagtttcggc tttcttatag
     5581 atcaaatagg aaattatcag tgatattcca gtaaagaata atgtactagc tgtcactaaa
     5641 agaaataggt taatcatacg atacactcct tctgtaatat tggtatcaga tacgtttaaa
     5701 tgcgtttctg agctgttttg gatcaatgga tgttttagcc ctaccctaaa atcaaagccc
     5761 agaaaagcat ttaaaaacgt tcgtacctaa tacgtttata ttcatataca tatacataaa
     5821 cataaacata aacgtataca cgtttatgtt tatgtattta agttttaggc tacttcctta
     5881 acatctcctg ctgatagtcg agtgaaccga gtgagtgatc catcttgtag tctaaaaata
     5941 aagctacgtt tgagtttata ctgataaaca gcgacatacg atccatccca gattgagtaa
     6001 gttttacctg caactaataa gtcatcaagg tttggtttat tgttttcttc tttaattcta
     6061 tctagttgtt gcttaaccat tttttcttga tcaccgacaa agaatttcaa ccattttttc
     6121 ggaattttct taaagtaagc aggactgtca tttaagaagt agattggttt gtacatttca
     6181 tctcgaaaga tgaagctgtc aatatcgtag gttccttctg catctttgaa aaggataata
     6241 ccataggtct tcgttacttt ggtatctacg atttccaaac catttttggc gtaccctttc
     6301 ttttcttcga tcacttcatt tgctgatgaa taagcaccat accatcccat aaaaaatcgc
     6361 tccttttgaa agttaattag cttgtttaca cttatattat acacaccagt gtgtataata
     6421 acaagagggt attgttcttt tttaggtaaa gtgggatcgt aaacagtgat tttgttgggt
     6481 taacaaaaaa acagacaagc cttatcagct tgtctgttac ttataaactt cacttctgtg
     6541 accaatagac aatataagaa ttgtcacttc atgctcgcta atatcagcta ttatacggta
     6601 ggcacctacc ctatatcgcc agtagcctga tttattagct gttagacctt taccatgttt
     6661 tcttggatca tcagtgccta caagattttt ctcgatccag tttaggataa cctttgcctg
     6721 aaacttatcc atttttttta gcgctttttg agcttcacgt tcatatctta cttggtaagt
     6781 catcttataa gcctaactct tttagcatat cttcgtggga aatactttga tcgttttcct
     6841 catgctcttt cattagttcc ttgtaggtgg caaagtctac taacgtttct gctgtttgca
     6901 gggaattact acgcataaat tcagaaatgt tatctccgtt gaattcagca actttttcaa
     6961 gaaaatcttt ttcttcttta gatacacgaa tacttattac tggcatagtt tatcaactcc
     7021 ttttcaatta cattgtacta cgattgtatg acaaatgcaa tagttatata tatgaatata
     7081 tacataaaca taaacatata catatatatg tttatgttgt agaatataaa agcaaatata
     7141 aatataaaca cgtttatgta tataaacata tacataaacg tgtttatagt gtataatgga
     7201 gttacaaaag gttaataagg gaggtttaat catggaaaag aagttgtcaa tcattactgg
     7261 aaacattgaa aaaggtggaa caggtaaaac aacaacaatc tacaatctag gtggatatgc
     7321 tgtcgaaaat ttaggtatga aggtgctgta tattgatgaa gataagtcgc aaaacctatc
     7381 aaaacgattt cctgatgctt tcagtaaagc gaaaagagag aatacaatca atcatttata
     7441 tcttcatgga tcagctgaac ctttagctgt aaatgagaac atggatattt tagtagcagg
     7501 tgaagattta aaacaagttg agatgcaaat gcgtgatatg cctaacaaca gaaccctact
     7561 gtttagatgg atggttgtta actatgaaca gcttaaagag aagtatgatc taattctgat
     7621 tgatactcat aatgatgcag gtctactagc tcaaaatgct tgggctgtat ctgatcttgt
     7681 cgtaggcatt tctgatccat ctaaagatgg ttttgaagct ttgctgaagc ttggtaaaga
     7741 tattgatgca ctagcaaaac agttgattga tattaagact ggtgaaacac tgatggaaac
     7801 taaatacgct acgatcggta gcaggatcaa acataatact aattcaagtc gtgagttcct
     7861 agaactggta ccaacgttat ctaattatct tggatatgta caagagaagg aattgcttaa
     7921 tgcaactagc ttagaaggta agaatattgc tgagttcgct aaggataaga atatctatcg
     7981 taagaataag gatttctttg aaagcacgtt tacgctatac aacaaaataa ttgatacggc
     8041 acaagaataa ggagtgattg aagtgaaaga aaatagattt aaaaaattcg ctgaagctga
     8101 agtagaagaa gttcctgaag tggttgaagc agttgaagaa cctaaacaaa cacctaaaga
     8161 aacaacaagt tctttggtag ctaaactaaa aagtgatagt tctgaaaaga agaaaaatta
     8221 tcaattcacg cttcaaccat cgcaaagaaa gaaactatct aaaatcagcg aaaagttagg
     8281 aattcgatca gattcagctt tacttggtga aatgatagat aacttgtatg atgaagtttt
     8341 tggtgagtga tcagtttaaa gctgatcact ttttttattg gaataagcgg ttaggtatag
     8401 cttaactaaa tttaatatac gtgtttatgg atatatacgt atacatgttt atatacatgt
     8461 atacgtatat gtttatgttt atatttgtac ataataaaaa ggctaggtat tgtaacctcg
     8521 cctttttatt tttaaagtaa gtctttgtta cttggtgcat tgaacccttt cagcattggc
     8581 ttttctttct ttgctgaatg atcggatgta accgttgaag gatgctcttg ttgaataggg
     8641 ttatgatcag ttggtttatt ctttacttct gctactttgc tatcgtttct atcgttgcct
     8701 gcttcaataa ttgtaacttc attgttcttt acacgccgat ataggttcaa agcttttttg
     8761 atcgtctgag agtatttaac gcctagtgtt tcatattcgg attcaagaaa ctctgctaaa
     8821 ggtaactcgc taaccttttc tctattgatt ataactacat aacgctctga ttttccctta
     8881 ttcatgttga tctccttcgc tgttttattg agttaaacca aacttgtaaa agccttttac
     8941 attagaaaat tcactgtctt caacaaacaa ggtatgcgga attttatctt ttacaagttg
     9001 tttgtcgata atagaagctc caccaccagt aaagacaact ttatctaagg ttgtgaagtt
     9061 tttgaatgta gctaaaactt ctgcaacaac catattggtg tatgttttta tttctctttc
     9121 aactacttct gaaatatcaa aactttcatt tccgcttggc ttaaatagat atacgccttt
     9181 ttctttgcct tctcggataa tcttttcaag ttggaaagca ttaggactga tcggaaggtc
     9241 aagcatattt acgatacgat tatataaagt aaaaatgccg gtatcctttt gttcacgatt
     9301 gttttcgtca atttcaaaat tttggacaac atcaaaaagt ttcgtgccac cgccaatatc
     9361 aacaaaaccg atcttctgac tgatcaatgt ttcatcttgt acactaccat catcagcaag
     9421 taggaggtta tatagtgttc caatcggctg tggcagtacc caaacggtat ctacgtttat
     9481 attataagct ttaccgtcaa cttcaactga atgttgtcgt gtggcttgtt gcatcacgta
     9541 tttgacgatt gtttcggtat agtcggatga aggcagacct aaaactagat tagcggttaa
     9601 aacgtcatca gattgatctt ctaaacgtgc taggttagct aaagagaaag ataggatatt
     9661 ttggtaggtt tcctgcttgt atctgctgat cccaaaacct agtgattcta tcacgtagtc
     9721 atcttttcct agctcgtgta tatccttccc aaagtaatag ctttcagtag tgtttactag
     9781 gctatattca gaaacgttta gtgatcgttt taatgaggaa cctatattag aaaaacgtcc
     9841 tgaattaatg atatttttgt tcaaaatacg agagggaagt gagagtggag aaacgtgtgt
     9901 actcatttct tttactgatt tattccctaa gtcaacggaa aacaataatg gttctttcat
     9961 ggtatgacct ctttctgttt gtgtttatgg tatttcggta cgaaaatagt ttacacctat
    10021 ttactattag ttgcaactgt ttataagaaa aaaatgcaat cataaaagtc taattaatta
    10081 taataaagtg taaataaaaa ggtgtaaata aatgcttgca aatttatctt attatgatat
    10141 tataaaaaca taaattatat taaggagtga tcctgatgcg attgagaaga aataagaatt
    10201 acactaccat tattttagaa gattttactg atcaagaatt acttattgtc ggtaaaacaa
    10261 aagcagattt tatcgaaaag ggaaatgctt caatagtaaa agcttcaaag attgctgtcg
    10321 aaaaatattt tgattcttta gaagaaagtg ctaaggaaat ttttaatgga gagaaagctg
    10381 gtagctatga gttagacggt attagattta gcttgatccc aacaactgaa aatattattc
    10441 cgaagaaatt atattatatc ttttcatctg aaaaacatac ttcaatgaag ccttgtattt
    10501 ttgattggca atggttgatt gatgctgtat tggatgtaaa atgttcaaaa tacaatatta
    10561 ctttggggtg ttaattatga gtatgctagt tagaaaatta ccattatctg taagagaatt
    10621 tctattttac ttggtctgct taatcgcatc agggtttaag ctgatcgaac atatttttat
    10681 ctttgcttat gtattagcaa tgatcgcatt tttatctggc ggttggttaa ttatttattt
    10741 gttaagctaa gttttagatc aaaaataatt aataaattaa acaataaaat aaccgttaat
    10801 aaatgtaatg aaaggacaac gcctaacaat cagcttgtaa taagattata aaataacatt
    10861 atattaaaat aagtgctttt taaaggacaa a
//
LOCUS       DAGCWU010000097        10760 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.097, whole genome shotgun sequence.
ACCESSION   DAGCWU010000097 DAGCWU010000000
VERSION     DAGCWU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.097"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            46..1272
                     /locus_tag="MBZ71_001888"
     CDS             46..1272
                     /locus_tag="MBZ71_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429840.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2122..3446
                     /locus_tag="MBZ71_001889"
                     /pseudo
     CDS             2122..3446
                     /locus_tag="MBZ71_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            3439..3786
                     /locus_tag="MBZ71_001890"
     CDS             3439..3786
                     /locus_tag="MBZ71_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429841.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            4101..4958
                     /locus_tag="MBZ71_001891"
     CDS             4101..4958
                     /locus_tag="MBZ71_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBT4429842.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            4978..5538
                     /locus_tag="MBZ71_001892"
     CDS             4978..5538
                     /locus_tag="MBZ71_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HBT4429843.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            6511..7278
                     /locus_tag="MBZ71_001893"
     CDS             6511..7278
                     /locus_tag="MBZ71_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HBT4429844.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            7304..7849
                     /locus_tag="MBZ71_001894"
     CDS             7304..7849
                     /locus_tag="MBZ71_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429845.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            7850..8416
                     /locus_tag="MBZ71_001895"
     CDS             7850..8416
                     /locus_tag="MBZ71_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429846.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            8431..8679
                     /locus_tag="MBZ71_001896"
     CDS             8431..8679
                     /locus_tag="MBZ71_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429847.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            8698..8937
                     /locus_tag="MBZ71_001897"
     CDS             8698..8937
                     /locus_tag="MBZ71_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429848.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            8954..9538
                     /locus_tag="MBZ71_001898"
     CDS             8954..9538
                     /locus_tag="MBZ71_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429849.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            9645..9926
                     /locus_tag="MBZ71_001899"
     CDS             9645..9926
                     /locus_tag="MBZ71_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBT4429850.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            9916..10257
                     /locus_tag="MBZ71_001900"
     CDS             9916..10257
                     /locus_tag="MBZ71_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4429851.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         4091 a         1399 c         2152 g         3118 t
ORIGIN      
        1 atttggacag tcatgtgaat atgagcttaa caattaaacg tatttatgat cttttatatg
       61 tttataaaca ttaatgaggt gctaaaattg acaacaataa ctatgacaaa aaaaatagaa
      121 gtatatgttg atggatcaca agaagaaagg gattcctttt ttgatttttt ttggaaaaat
      181 agtagagaat atgtaaaagc gttgaatctg gtatataaac agctaatgtt tgaagagttg
      241 ataaaagatg caattaaaga aatggatcaa ggttatgctg agagagaaga aagtattcaa
      301 aatcggattg aaaagttagc atatactgat ccaatagatc aaaaaaaact tgataaagca
      361 aaagctgaat tatctaaatt gagaaatgaa aaaaataaag aagcgagaga aatattaacg
      421 caagcgatag ggttgaagag tcaaacgaga acacgagatg tagcaaaaaa attagacttt
      481 gactttggag attttgttga ttccgcaaat atgaaagcta gtaaagattt tggcaatgat
      541 tttatcggaa ttgtgaccgg acaaagaacg cctagaatat ataaaaatag aaacaaatat
      601 ggattactac cttttagagg tcgagatatt aaaatccata aagatgataa gttttatatt
      661 tatttaccta aaggctttag gtttaaagtg aatttaggta gcaagccaag aaaagcaaga
      721 gatgttgctt cagttttaca acacattatg gatggcgatt acaaattaaa tcaaagtaat
      781 cttatttttg atggaagaaa attatttttt aatctaacaa tttcttttga aaaaagctat
      841 aatgaaatgg aattcaagaa aggtaatgaa ttgatcgtaa actttgcaga tttgtgtagc
      901 ggtgaatgtt taattaatga taaatctgta atgtcttttg gtgatagcca atatataaga
      961 aacttcaaaa gtaaaatttt agcaatgaaa tcaaaagaac aaagccgagg agtcttttct
     1021 aagggcggtc atggtcgaaa aagaaaatta gatcaagatc gatgggaagc aataaagaaa
     1081 agagaaagta attttgttaa aacttataat aatcaatgtt ctgcaaaaat cgttaaaact
     1141 gctattcgca ataaagctca aattattcgt atcattgaac cagataaggc ttatggcgaa
     1201 tgggcttact atcaattgaa agaacaacta gtaaacaaag gaaaaagata tgggatagaa
     1261 gtcagaagtt agtaaattaa acaatcgttg atttggcaac gaaatagtga gggaaaatcc
     1321 actcactgaa gggtaaactc cgtcaagttg tgtgaccaga cggaaggacg aatgttgttg
     1381 aggttttaga accatataaa acagcatgtt tctcaaattt ttaggataag ggaaattgct
     1441 agcagtttgt tttagaacca tataaaacag catgtttctc aaatgagtac tttaaattaa
     1501 ttgatggcgt tcctgcttta gaaccatata aaacaacatg gatttaaaac aagaagtaag
     1561 tcagttctcc ttttacttta gtaaggaaaa ataaagtatt ctcaataaaa cttaaaataa
     1621 gcaaaccttt gttttaatag ctaatcgaag taaaacccaa agccatatga acccctgaat
     1681 cagcgaaagt ttatgatgat ttggattact aatagtttag gaattagatg gtaaatctaa
     1741 attaaacaat cgttgatttg gcaacgaaat agtgagggaa aatccactca ctgaagggta
     1801 aactccgtca agttatgtga ccagacggaa ggacgaatgt tgttgaggtt ttagaaccat
     1861 ataaaacagc atagttctca aattgtttaa aatgattgtc aagttaaaaa aatctgggat
     1921 catataaaat agtatagttc tcaaatatga ttagaaaccc tattaaagag caatattaag
     1981 ccaacttact taaatcgttt aattaagaac aataatctct tttagtcaga cttttagtct
     2041 gacttttttg tatttccaaa atttattctt tacaaaaacg aacatacgtt tgtataattg
     2101 cttgtgaatg ggggaataaa aatgtttgat tacagtagag aaccggtgaa tgactatttt
     2161 ttaatcgaca tgaagtcttt ctatgctagc gtggaatgta ttgaacgaaa tttagaccca
     2221 atgactacac cgttaattgt catgagtaga tcggacaaca ctggttcagg tctgactcta
     2281 gcttcttcac ctactgcaaa gaaaaaatat catattacta atgttagtag agctactgat
     2341 ttaccttatc cattaccaaa agaacttcat attgttccac cacgaatgaa cttatatata
     2401 aaaaaaatat ggaagttaat cagattttta gaaagtatgt cgcagatgaa gatttattga
     2461 tttattcgat tgatgaatca attttaaaag tgacaaaatc cttaaattta tttactagtt
     2521 caggaacaag aagcgagaga agaagagaat tagctaaaat aatcaaagat gagattcaga
     2581 aagaaacagg tctaatagca acagtcggaa taggagataa cccattatta gctaaacttg
     2641 cactggacaa tgaagcgaag agtagtaaag aatttatagc tgagtggact tatgagactg
     2701 tacctgaaaa aatatggaaa ataaacgaga tgactgactt ttggggcatt ggatcacgca
     2761 tgaaaaaaag attgaatcat atgggaatta tgagtataga agatttagca aattggaatc
     2821 cctacacaat taaaagtcag ttaggtgtaa tcggactcca gttatatttc cataccaatg
     2881 gaattgatcg tacggatatt gctattccac cagaaccaac caaagaaaag tcttatggga
     2941 attcacaagt tctaccaaaa gattatacga ggagagaaga agtagaacta gtagtcaagg
     3001 agatggcaga gcaagtagcc attcgaatta gacaacacaa ttgcaaaact ggttgtgtac
     3061 gcttagcaat tggtacgtca attttggaag aaaaatcagg tttctcgcac cagatgaaaa
     3121 taccaattac agataatacg aaagaattac aaaattattg tcttttttta ttcaataagt
     3181 tttatgaggg gcaggaagta cgacatatag gagtaactta tagcagatta gtatacacag
     3241 attccctaca attagattta tttagtgatc caagaaaaaa gattgctgaa gaaaatttgg
     3301 ataggattat tgataagatt agacaaaaat atggatttac agctattata catgcttcat
     3361 ctatgttaga aggtgcaaga agtattacga gaagcacttt aataggagga cacgcaggag
     3421 gattaggagg tttgcataat gattgaatat aacgatagag gacgtttaaa atggtcggga
     3481 ttttatttgt ctgatcatac tgaaagaatc aatgcagaca atgtacaacg tgctaatcat
     3541 aatttggcaa aagaacaaat gacgactgaa gagattgcga aaatactgag aactgctgtc
     3601 ataaaaaata gacccgtctg cattcaaaaa gaggaacgaa acgctgaagg ttactatcca
     3661 gatgatgtag taggaaaaat tattggatat gacgagcttg gtgtatttgt ggaagaagaa
     3721 aaagttcact atgatgaaat taggaatgtt tctttctcgg aattattaaa atggagtcag
     3781 atatgaacat tattaaaaaa tacgaattcg gttatattac gtatgaacag ctaatagagg
     3841 aattatgggg atatggtcaa caattaatta atgaagtggg gattgattgc ttttgtttct
     3901 atattgaatc cggagcagga taccataaga acagatacta tatatttcca tatccaccaa
     3961 aatagtctct tagatatttt taaattttct ccaaatactt tcaattattg tgaaatattt
     4021 ccctttaatt aatcaaattt tttataaaag taatatataa aataaattgc ttgaagtatt
     4081 atatttagat atcataattt atggaagaaa aattaattaa aaaggatgag attataatgg
     4141 ggttactggt tccgagagct aatacttata ctataccttt aacgaaacgc caattaattg
     4201 ctaaaagata tcaaaggatt actagagcca tcaatagaga gttttggaat tctgaaagtg
     4261 atactgctca cagtttatac gtaggttctt atgggagagg aacggcaatt agtactagtg
     4321 atatagatat aatagttgaa ttaccgatgg cagaatttga cagattcaaa aattatttat
     4381 caaatggccc gtctaagtta ttacaagtta taaaaaacgc atttcaggaa atactaccaa
     4441 atagtgatat tagagctgat ggacaagttg ttaaaataaa tttccatgat ggaatcaaat
     4501 ttgaaatagt tccagctttt aatgaaaaag attattgggg tgagtcaaaa gggtttattt
     4561 atccagattc aaacatggga gggaactgga aagctacaaa tcccaaaaaa gagcaagaag
     4621 ccatgaaatt aaagaataca aaaagtaata atttattata tgctacgtgt aaacattttc
     4681 gacatgtacg agatacagaa tttacgagtt atcatctttc aggaatagta attgacagtt
     4741 tcgtatatga ggctatggga aattggaaat tcgttgaaaa taacagtggg ggtcaaaaca
     4801 ttagttctgt atcttatgag acagctttat tagaatacta taactcacat aaagttatgg
     4861 gcggtttaaa tttatactct cctggaagca atcaatttgt taattctgat agtagtatca
     4921 tatgtttaga aaaagtatta aaaaaaatag ctttataaag ttttatggag gaacaacatg
     4981 ggagacgaaa aaaagcgctt ggaagttcaa ttacgtgaag catatggacg agtaacgtat
     5041 acttacacca ctcatttaaa gcaagccgat aatattattt ctgacaataa aattttaagg
     5101 tgtcttcaaa ttggtttgtc agcccttact actggagggg tacttagtac cattattact
     5161 gataatagtg agttaacctt tgttgctgga ttatgttcaa caatttctct tggattaaac
     5221 ttatacttta agaattttga tcttgctgaa gaagctaaaa atcatcaaat tgttgccgat
     5281 aaattatggt tgatcagaga agaatacgta agcttactaa cagatttaaa tcgtttaagt
     5341 gttgaagata ttgtgaaaaa aagagatcaa ttaatgaacg aaactcatga agcatataaa
     5401 aatgccccta aaacaaatag taaaagctat gaatcagctc agaaagcatt aaaatctgaa
     5461 gaagagcaat tttttacaga agaggaattg gataagatgt taccagagca tcttagaaaa
     5521 aagagttcta aatattgaac tcttttttta tgcttaaaat tctcttgtaa tttttctatt
     5581 catctatatt taagaagaaa tatgatgata gaaacatctc ctaaagccac cgcaagccgt
     5641 tctaagcata gcttagcccg acattgctag atatgctcat ctcaccatga acgtttccta
     5701 aagccaccgc aagccgtcct agccattgcc tagtccgaca tcgctagatt agtgcatctc
     5761 gccataaaag ttttctaaag ccaccgcaag ccgtcctagc cattgcctag tccgacatcg
     5821 ctagattagt gcatctcgcc ataaaagttt tctaaagcca ccgcaagccg ttctaagcat
     5881 agcttagccc gacattgcta gatatgctca tctcaccatg aacgtttcct aaaaccaccg
     5941 caaaccgtcc tagccatcgc ctagtgccta gtccgatttt gcttgatcgg ttccatttct
     6001 gtttatttag tgggtcaaca ccgtaatctt aaatagccat cgcctattta agattactag
     6061 gtctgctttg aaggtaaaaa gtctttcatt cttcaagact tttaggcttt caagctacgc
     6121 ctgaaagcat tgaaattcat cgctctttat ttggttttgc catttataat tccttacgcc
     6181 cattatcttt ttcgcctgtc ttgccttgtc agaagtgttt agagcttatc tcatctttgg
     6241 attaatacaa gtggcgtaaa tttttttcta aacgcaagcg ttacgaaaat aatttactta
     6301 aacacttgta ttaatccggc agataagaac gcattctaat cactcccgac aaggcaagac
     6361 aggcgaaaaa gataaataaa tgaacgaaag gaagaaacat aaaatgcaaa accaaatagc
     6421 gatgaagaaa aaaccacctc cctctaaaac aatccaaaca aaaaagaatt ttgaaaaacc
     6481 aaaaatcaaa aacagaaaga agcgttcaac atgagagata gaattttaga agttaatcaa
     6541 gaaatggtaa acaagttaac aaatatcagt gggaactatg aaaagtacga tggtacttta
     6601 cttgataatt atatttttca cgatacacaa aatatcagaa ttggcagaag taaaccgaga
     6661 gcgttcattc tcatcaaaga gaaatatttg aatgaatgga atagcaccta tgaaatggtt
     6721 atgactgata actttgaaaa ggtattggat ttcagaaatg ctttgttggg ttctgatatg
     6781 ggaaatagca gtcttgaatc tattgaagtt gaaaacaaga ccgagaatga aggtgaagga
     6841 ttgattaaga atgtcctgaa aatcgaacaa aaatatatca gtgaagatag aagtttttat
     6901 agagtcacaa gctcggttgg tctagcaaac tttgctacta acttaattgg aaacaatgct
     6961 agtgaatctt tgctagtgat gtgtctgaat acaaagaatg aggtcattgc ttatagcgaa
     7021 gttttcaaag gtggaattaa tcaatcagta gcgataccaa gagagatttt tcaactagct
     7081 ttgctaaaca attcagcaaa aatcgcagta tcacataatc atccgagcgg atatgaattt
     7141 ccaagtgaaa atgacaaaag ttttactacg aatttgaaaa acgcaggaaa aacaattggt
     7201 attgaattgt tagaccatat catcgtaaca agtaaaaaca caagctatta ctcatttcaa
     7261 gaaaatggtt ctttataaaa attagagaaa agaggaaaac aagatgggcg aaagaacaca
     7321 agcgttaatt acagtcaaat atggtaacag caaaacgaaa gtaagcgtgt caattcatta
     7381 tcagtgggga tatggtcgat tcatgttgat ggacgttttg aatcaaggaa tatatttaat
     7441 caataaagca tatgacttac gagatgaatc aagagaagaa ttagcaaaag gaatctttga
     7501 tagaagtaca ggagttatca attttgttac ctatgaatct ggagtgagtg cttatgatga
     7561 aacagggttt ggtgggcgct atgaacctta tcaagaaacg attgaagatg tgatgaagtt
     7621 ttcagataac aatgatggct atgctcattt agagatggtg ttcgatgaaa atggatttag
     7681 tctagatgat aaaaccagat tgcgattgtt tgattttgag ggtcaacaga taggactaaa
     7741 caagttttgt gaaagcggaa aagagtttgc tacgaaagat tttattaagg gatacagatt
     7801 attgctagaa aattatggaa taaacctaga agaagagagc gtgaaataag tgtttgaaaa
     7861 aagaaaagac cagttcccta atttagaagc gaaaagtcaa aaagtgattg atggtgaaat
     7921 gattgaatta ttccaaaaaa gttatggaac catagaaact accgcaagtt gcaaatacta
     7981 ctttcaagga aagagaaaca cgtttaaagg ctttgccgat tctgatgaag aattggcaaa
     8041 gaaaatcatt gattggcttg tcagcagtga acacgtagca agagaagata ccgttagaac
     8101 aacgattgag ggtgaagtcc taacaggtac tagatgtgtt tgttgtaatc atgaatttgt
     8161 agaagatgaa ggttgctacc gaatcaatga atatggtgat tttatttgcc agagttgcca
     8221 cgagatagaa tttccaaccg aaagaagttg gtttgaatac atgctacaag attatgagat
     8281 ggaaacagaa aatggtgaag tagttttttg taccaaagag gaattgagta agagaagcga
     8341 tgatgaaatt gaagattggt gtgtgtggtt agtagggaca gatgattgcg ttgcatatta
     8401 cacaacagca gaataggagt gtgaatcagc atggaaggaa tggattttat agatcatgaa
     8461 gatttgattg attttggtta tacatggaaa ggaatggtag gtattagcag aagtcttgct
     8521 aatgcctttt acgaaagaaa ttatgcagtc tatgtccttt atgatgacaa tacagaaagc
     8581 cttgttgatg aagagtataa attggattta gagaatgttt tatatgggat agaaaaagaa
     8641 gatttagcaa aatatatttt ttcatggttg ggtcaataaa cgaaagaggg tagcaacatg
     8701 acaaaattca aaagtaaagg tatgcaatgg tgttcttatt attatgttgg gaatgatgaa
     8761 gagcttttga gaaaagccaa acaaaaaggt tatccagata agatgttaaa acagattggc
     8821 tattacatgc cagaagagag aatagaaaaa gaatcttgta aggttctttt tgatagtgaa
     8881 cagctattag ttttgttaac tcaaaataaa ttgatttgtc aatttgaaaa taattaatat
     8941 ggggtgaaga accatgaatg agaataagat ggtattaagt gaatacgcaa gattgagaac
     9001 cattaattgg ttgaatggta tgcaaagtag tggcgctttt gatggatttg atgaggagaa
     9061 tatggacttt gaaagtttat cgaatgagga tattagatat tattttatcg aatggtattt
     9121 tacagaacca gatgaagagg tggaagatga accagaatta attttagaac gtcaaagaat
     9181 tttattcagt gaacaagata gtaagaggtt gtatggttta tatgacttac aactgaaagg
     9241 attaaaagcg cagtatgttg ctgatagtta cgatgatatg gtagaacagg gggcagatag
     9301 ggttgtcact tattcagaag atgatgaaga ggaatgggaa aactattcag atgatgagat
     9361 atgctttaat gatgggtata tgcctattga aatcaccgaa gaagaatatg ataaagtgtc
     9421 ggatagcgat gagattggta tgtgtacggt tgtaagtgga ttggatatag atgtccaaag
     9481 agtagaggtt gcataccaga agatgaaaga acgtataaaa agtaaagtag aatcttgatt
     9541 tgattggatt gtttgcaaag tagctaatta agagtataat ataatcataa atgtagttaa
     9601 tggatataat aatgagtata ttatactcaa aagaggtgaa cgaaatgaag gtaaaaaatc
     9661 caaaagaaaa aagcagaatt caagtcggaa tagacaagga tttaaaagaa aacgcagaaa
     9721 tgatattgga agagctaggg ttaaatccaa caacagcaat cacaatcctt tataagcaag
     9781 tggtcgctag aggtgagttt ccagtagaga taaagctaag cgaagaggaa aagcaaggaa
     9841 tcagattgca acagctaact atggatatgc cagttgatgt tctagataca gatgaaaagt
     9901 tagaagagtg gttcaatgaa gcctaatgag atagtaacgg tctatgttgc atttacagat
     9961 aaaaatggtg gtaaaagaag acctattttg gtggttagtg ataaagaaga tagagtagaa
    10021 ttttttggaa tcacaagtca atatgagaat aaatcagacc aaataaagag agtgtatttt
    10081 cctattgcag aatgggaaaa agtaggtcta aagaagcaat catggattga cgtagggtct
    10141 ttaaaggcta ttcccaaaag ccgagagaac atatcattta aaaaggttgg tatgctatca
    10201 gctattgata cgaaaaagct aaataggttt atcgagagat tacaaagaaa agtctaacaa
    10261 ggcttttctt tttttgttta tatgtatatt aagatatacg tttatataaa cgtatttatg
    10321 tatattaaaa tacacgtaaa tatatacgtg attataaaaa ttaagaaaat attaagtatt
    10381 gttaaatagt gataattagt aatatacgaa ttataagata cattttatat ttaatgtatg
    10441 taaataaata aaatgactga cggatattga atagtaataa ataacaataa ttcagacgga
    10501 tacagtgagt gaagtagctt gctacgaacg aacaggcaga atgaaacggt agtttcatta
    10561 gagcaagtcg cttgcgacgt agcgttacca ctgatgtaac tggcaagtta catagagaga
    10621 gtttgcttgc aaacgaacgt tacccacttg aatagaaaac aaaaataaca tattagtgaa
    10681 ttttgaatag gagccacaac tcaaccaacc gcttgcggtg ggtgagtccc acggaggaaa
    10741 agacgaacga acggtcgcgt
//
LOCUS       DAGCWU010000098        10752 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.098, whole genome shotgun sequence.
ACCESSION   DAGCWU010000098 DAGCWU010000000
VERSION     DAGCWU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.098"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            163..978
                     /gene="thiD"
                     /locus_tag="MBZ71_001901"
     CDS             163..978
                     /gene="thiD"
                     /locus_tag="MBZ71_001901"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBT4429852.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1307..2758)
                     /gene="cls"
                     /locus_tag="MBZ71_001902"
     CDS             complement(1307..2758)
                     /gene="cls"
                     /locus_tag="MBZ71_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBT4429853.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3099..5765
                     /locus_tag="MBZ71_001903"
     CDS             3099..5765
                     /locus_tag="MBZ71_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4429854.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6129..6881
                     /locus_tag="MBZ71_001904"
     CDS             6129..6881
                     /locus_tag="MBZ71_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4429855.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6903..8057)
                     /locus_tag="MBZ71_001905"
     CDS             complement(6903..8057)
                     /locus_tag="MBZ71_001905"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBT4429856.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8242..10683
                     /locus_tag="MBZ71_001906"
     CDS             8242..10683
                     /locus_tag="MBZ71_001906"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBT4429857.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3503 a         1916 c         2306 g         3027 t
ORIGIN      
        1 acgaccacta ttttctgatt tattgcaaat gttttttcaa ataaatttga ttaccaagaa
       61 aaggtagtgg tatatttact gagggatact ttgaaaaaca tatgatagaa acagtaacag
      121 tattatgaaa aatcttttta taaaaatgga ggaatcgatt tgatggaaga tcagtatgta
      181 caagcaatga cgatcgcagg atcagatagc gatggaagcg cagggatgca agcagatatg
      241 catacattct ttacaagaaa tgtttacggt gtttctgtca tgaccgcatg tgttgcaggt
      301 aattcttacg gaatcggagc aagtgtgaca ttgccaacag attttatcga caaagaattt
      361 gaactgattg cgaaggactt tcaaatacgt gcagctaaaa caggtatgct agcagattca
      421 aagttgattg agacagttgt aaaaaattac caaaaatatg attttggacc attagtcgtg
      481 gatcccgtaa tcgtaacgaa acatggaaat cttttactag aagaatctgc tttgcaaagc
      541 ttgaaagaaa agcttgtccc tttggccgaa gtactgacac ctaattttta tgaagcagaa
      601 aagctgactg gacggacatt agcagctgaa gaagattttg tgcaagcagc aaaagaactg
      661 caagatatgg gtgcaaaaaa cgttatgatc aaaggacatc atgctaaaaa tgcgcagcaa
      721 gaaacagtca atgactacgt attgttcgaa tcaggaaaat cattttggtt aaatgcacca
      781 ttttatccta ccgaccggat caacggaact ggcgattcgc tatctgcatg tattgcagct
      841 gagttagcga aaggaaaaac agttgaacaa gcgatccgat tggcaaaaga ctatgtaaac
      901 cgtgcaattc gtgaagaaat aaatgtaggg cacaaatatg gccccatcaa tcattgggca
      961 gctggtgaat tagagtaaaa gaaagaagtt gtgaaaaagt tgccctgcat caagtaataa
     1021 gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gttggactta ttaccgcaga
     1081 tgcaagcttt tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgtcca gctccgagtc
     1141 acaaggaaca agttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg
     1201 aggagttggc aggagaacac cgtttgttcg gaagaaggga ctgtgacaag atttttgtca
     1261 cagtcccttt aatcttagta tatttttctc tacttgaata aaaaaattat aggattggag
     1321 aaaataatcg agaaatttgt tgcttgaata atagccagtt agacatttcc ttgactgttt
     1381 cagttgtcat ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca agttcttcta
     1441 aaaactcagg atcgtaaatg acagcacttg tttcaaaatt caatcggtag ctacgaatat
     1501 cttgatttgc tgaacctacc atgcagattt catcatccat tatcaatgtt tttgcatgga
     1561 ggaagccgtt ctcatagaca aggatttgta cattttcctt gatcagctgc cgagcataat
     1621 attgtgttgc tcgataaata aaaggatgat ccggtttgtt gggaatcatg attttcacat
     1681 ctacaccaga ggcagttgcg atttttaaag cagcaatgac actttcatca ggaacaaggt
     1741 aaggtgtctg tatccaaaca cgttttttag cagaagtaat caatttgata aatgaaagct
     1801 tgatttgttc cctttcgtta ttcggtccgc ttgaaacaag ctggatggat gtatcagcaa
     1861 gcttagattc atctcgatct gcttttttaa agaaataatt caaatgatag ccgacctttt
     1921 tttcttcagg taccgagacg ttccagtctg ttaagaagcg aagctggagc aaagaagaag
     1981 cggcaccgaa aatccgtata tgcgtatcgc gccaatagcc gaattttttt gttgtattta
     2041 catattgatt ggcaatattg aaaccaccgg tataactaat ctttccatcg atcacaacga
     2101 tttttcggtg atcatggtaa ttcaatcgga aacgaagtaa tgccctttgt gaggtaacaa
     2161 atgtatggac atgtccacca tttttgatta gacgattgaa atcttttgct tttgtgccat
     2221 gagagccaaa tgcatcatat aatattcgaa cttccacgcc ttctgcggct ttttcttcta
     2281 aaacatgtaa gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat tcgatatgga
     2341 tcgaatgttt ggcttttttt agatcttgaa gcagtgaatc caatttctct tgtccgtctg
     2401 tgagaagagt gacagaattc attcttgtca gcggcatacg atttaatgaa gagaagaagt
     2461 caacaaattg ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca atggtatctc
     2521 ctctaaaaga ctgaaagttt tctaattctt tcaagtcact ttgtcggaga tagaattttt
     2581 tcttatccgt taatccacgt ccgaaaaata aatataatac aaagcccacc ccaggaaggg
     2641 caaacaatac taagagccat gcccaaatgg ctgctacatc tcggggtttg atcaaaatag
     2701 ccaccaaagc aataagtgca tttaatagat aaagggcggt tataatacta gataccacaa
     2761 aaatgttcct ccttacatac aataacatta gtatagcatg atagaagaaa gaagcctacc
     2821 aaggtgaata tacttttcac tcctttcatg aaaatgtagg taaatcgggc taaagtagtt
     2881 gctttttgta ggttaataag gtaggatatt agtacagttg aatatctcct taggggagta
     2941 accggcaacg tttgttgcga taatatcaac agtaagttaa ccaaatggtt ttctggtatt
     3001 gtatgaaatg gtgagaccta agacaaatgc gtatttgtct taggtctctt ttttttcaga
     3061 caggactagg gccaagatca aaaaatgggg gatgaaattt ggaaaacaag gaacaagcac
     3121 aacaagcggt ctataaagaa acgatcgact cgttgatgca gcaaacgcag agctcaaccg
     3181 atggattgag caccacgcaa gcgaaggaaa aattagaaag agatggcttg aaccagctaa
     3241 aagaaaagcc gaagaagcca acttggaaat tgttcttaga aactttcaaa gatgcaatgg
     3301 ttatcgtatt gctgattgtc gcactgattc agatggtcat gggtgactac atcgaattct
     3361 tagttatttt tgcagtattg atgttgaatt ctataatcag tgttgtccaa acaaaaaaag
     3421 cagaaggttc tttagaagcg ttgaaaaacc tttcagcgcc cgatgctcga gtgattcgtg
     3481 atggtaagga aaaaacgatt ccggcaaacg aacttgtggt aggagatatc gtgttgttag
     3541 acgcaggcga ttatgtacct gctgatggac gtttactaga tgcgggatca ttaaaagtag
     3601 atgaaggtat gctgacagga gagtcgactt ctgcagaaaa agaagtaacg gatatcacaa
     3661 gtacggtacc aatcggagat cgtacaaata tggtattcag tggaacgatc gtaacttacg
     3721 gacgcggtaa attcttagtg acttcaactg ggaataatac ggagatcgga caagttgctg
     3781 gccttttgga atcaacaact gagcaagtca ctccgttaca acgaggtctg gataaattca
     3841 gtaaaaaact aagtttggct attttggcat tgtctttatt gattttaggt atccaactgt
     3901 ttcgtattta tcttggagaa ggtacaggag atatgactgc agatatcgtc agcgcagtga
     3961 tgtttgccgt agccgtagcc gtagctgcta ttccagaagc attacagtct atcgtaacaa
     4021 tcgtgttatc tttgggaacg aataaaatgg caaaacgtca tgccattatt cggaagcttc
     4081 cagcagttga aacacttggt tcgacaagta tcatctgtac agataagact ggtacattga
     4141 cacaaaacaa aatgacagtt gttgactact ttttaggtaa tggcgatact ggggatttca
     4201 caaatgaccc ttcaaaatgg acagctgacg aacgtcgctt gatcgaaatc agtgttttag
     4261 cgaatgatgc atcgatcagt gaagacggta ctaaattggg cgaccctacg gaagtagcgt
     4321 ttatcgactt tagtgaaaaa ttaaaccagc cttatcagga aatccgaaac aactacccaa
     4381 gacaagcaga acttcctttt gactcagatc gaaaattaat gtctactgga cacacaattg
     4441 ataacaaaaa ttacttgttt gttaaaggcg gaccagatat cgtcttccaa cgcagtaaaa
     4501 aagtattgct aaatggtgaa gtcgttccga tgacggatga attgatgaaa aaattccaag
     4561 agcaaaatga agctttttca acacgcgcat tacgtgtact agcttttgct taccgtccac
     4621 ttgataattt tgatttatca tttgaagatg aagacgattt gatccttgtt ggtttgcttg
     4681 ctatgatcga tcctccacgt gctgaagtga ctcaggcggt aacagaagca agaagtgcag
     4741 gaatcaaaac aatcatgatt actggtgacc acaaaacaac agctgttgct atcgcaaaac
     4801 aaatcgggat ttttgaagaa ggaaacctgg ctttgaccgg gacggaacta gatgaattga
     4861 cagaagatca actaaacgaa gttttagaaa aagtcactgt ttatgcacgt gtttcacctg
     4921 agaataaaat tcgtatcgtt cgtgcttggc aaaataaagg tcatatctca gctatgacag
     4981 gtgatggtgt aaatgacgca ccagcattga aacaagcaga catcggaatt gctatgggaa
     5041 cgggtacaga tgtggcaaaa gatgcagcag cgatggtatt gacggatgat aactttgctt
     5101 ctattgtcag cgcagtagaa gtaggacgta atgtgtttga taatatcaag aaagctattt
     5161 cttatctatt tgcaggaaat ttaggtgcga tcattgcgat catctttgcc ttagttgctg
     5221 actggtccaa tccatttaca gcattacaac tgttgttcat caatttagtc aatgattcac
     5281 tgccagcaat tgcattaggt acagaaaaag ctgaacctgc tacgatgaac cgcccgccaa
     5341 gagatccaaa ttcaggaatc tttacggaca aaacattgat ttctgtaacg tatcgtggtg
     5401 tgctgattgg gattgctgtt atctttgctc aatggcttgg catgcagcat tcacctgaat
     5461 tcggtgtagc catgtcattt tcaacattga tttggtgtcg gacattgcaa acattcccag
     5521 cacgttcaaa cacacaaaca gctatccaag cagggttctt ctctaacaga agtgtcttac
     5581 tagctgtagt tgtatgtagc gcgctttact gcttaacaat gatcccagga ctgaaagaag
     5641 tcttctctat cccatcagca tttggattga cagaagtctt tacatgtatg ggaatcgcac
     5701 ttgtgacaat tgttttgatg gaaatcacta aattgatcat tgtacgagta caaggaaata
     5761 aataaaacga atcaacgagg ttgtgagcct gccgtttaat tctgagtcac aaggtgcaat
     5821 ttcaacaaac agcttctcat gtttgttgaa tattgtgact tgtgaccgaa gagttggcag
     5881 gagaacaccg tttaaggaag aagttgtaaa atcagcattt tgagctaata ctgagggtca
     5941 tctgattgga cactgtttat tcggaaagag ggactttgac acgactttgg tcacagtccc
     6001 tttttttatt tatgatagac ggaaagccaa tcattttatt attcatcaaa agagatagaa
     6061 tagataaaag aggagaatga tttattccga aaagtgaaaa catataacaa aaaattagag
     6121 gaaatcaaat gaatgaaaaa gaatgggatg aatttgctga agaatattat gaaatccagc
     6181 aagaatcgca agtaactatt gctgaagatg taaaaaatta tctgaaaacg gagaaagtcc
     6241 tgcctgctga aacagcagca gacgttgctg gaggatctgg cagatttctt tccttagcaa
     6301 aagaaattaa tcgctatgat ttgatcgatt tttcttcagg aatgctagaa aatgcaaaaa
     6361 aagaagcaga aaaaaatgag ttgccaaatg tttcctttat caaacagtcg ttagaagaat
     6421 ttatccaatc aaatgagaca tatgaaatgg tttttacagc agcaaatcca gcattggtac
     6481 atcttattca attagaaaaa ttgatgaaaa agtcaagcaa atggtgcgtg ataatcagag
     6541 tagtggaatc aatcgatgat gttttttatc ctatcgaaaa gcaactagga atgatagaag
     6601 aagatcctaa tacatcgcca gttgtaatgg atcaatttga aacatactta attgatgaag
     6661 gattcaaagt gaagaaaaaa gattttactt attttacaca ggaatcaatt actgaagaat
     6721 tgatgaagct atactatgac gcgtacgcaa aagatcctgt tgtgaccagg tacattcgca
     6781 aactattcag tgaaaaacga gaaatcctta gtacgactaa gctgacctat agactgctcc
     6841 tttggtccgc agataaaaaa agaacctttc acgactcgtg aagggttctt tttatgattt
     6901 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
     6961 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
     7021 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
     7081 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
     7141 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
     7201 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
     7261 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
     7321 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
     7381 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
     7441 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
     7501 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
     7561 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
     7621 gccaagccgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
     7681 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
     7741 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
     7801 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
     7861 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     7921 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     7981 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     8041 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     8101 gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt
     8161 aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
     8221 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
     8281 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
     8341 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt
     8401 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
     8461 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
     8521 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
     8581 ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa
     8641 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
     8701 catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag
     8761 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
     8821 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
     8881 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
     8941 ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt
     9001 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
     9061 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
     9121 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     9181 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     9241 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     9301 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     9361 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     9421 gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata
     9481 gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa
     9541 gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc
     9601 gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca
     9661 tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca
     9721 cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg
     9781 atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct
     9841 tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt
     9901 ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac
     9961 atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa
    10021 gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt
    10081 gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa
    10141 atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa
    10201 gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt
    10261 agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg
    10321 cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta
    10381 ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat
    10441 tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct
    10501 ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca
    10561 ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt
    10621 gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa
    10681 taaaaaagca atatctttag aagaataaag caagatttac agttatttta tttactgcag
    10741 atcttgcttt tt
//
LOCUS       DAGCWU010000099        10620 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.099, whole genome shotgun sequence.
ACCESSION   DAGCWU010000099 DAGCWU010000000
VERSION     DAGCWU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.099"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="MBZ71_001907"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="MBZ71_001907"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBT4429858.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="MBZ71_001908"
     CDS             2714..2938
                     /locus_tag="MBZ71_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429859.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            3055..3552
                     /locus_tag="MBZ71_001909"
     CDS             3055..3552
                     /locus_tag="MBZ71_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBT4429860.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            3639..4031
                     /locus_tag="MBZ71_001910"
     CDS             3639..4031
                     /locus_tag="MBZ71_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4429861.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            4015..6462
                     /locus_tag="MBZ71_001911"
     CDS             4015..6462
                     /locus_tag="MBZ71_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4429862.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            6465..8648
                     /locus_tag="MBZ71_001912"
     CDS             6465..8648
                     /locus_tag="MBZ71_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4429863.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            8645..9646
                     /locus_tag="MBZ71_001913"
     CDS             8645..9646
                     /locus_tag="MBZ71_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBT4429864.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            9661..10575
                     /locus_tag="MBZ71_001914"
     CDS             9661..10575
                     /locus_tag="MBZ71_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4429865.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
BASE COUNT         3527 a         1946 c         2241 g         2906 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat aactggtttc tatcaaacgc
     2761 tcaatcactg gtgcttttag caatcgttgt gattggcttg tatcttggct tcaagcgtga
     2821 gtttagcaaa ctgattggct ttttaattat tgcgattatt gcggttggct tagtcttcaa
     2881 cgctgctgga gtaaaagaca ttttactaga gctattcaat cgcattattg gtgcttaaat
     2941 aaaaccgttc ttttgtggaa tataagtggt tttcttatgt tccgcaaagg aatggtacac
     3001 caaacgaagt gcggtaggga tttttgaatc tctacaaaga aaggacgtga atatatggac
     3061 gatatgcaag tctatattgc gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg
     3121 tggtttacct ttcccattga ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa
     3181 tatgaggaat acgccattca tgattacgag ttacccttta cggttgacga atacacttct
     3241 attagcgaac tcaatcgcct atgggaaatg gtatcggagt tgccagaaga actacaatcg
     3301 gagctatctg ctctgctcac tcatttttca agtattgaag aactaagcga acatcaagaa
     3361 gatattatca ttcattccga ttgtgatgat atgtctgacg tggcacgcta ctacattgaa
     3421 gaaacgggtg ctttaggcga agtgccagcc agtcttcaaa actatattga ttatgaatcc
     3481 tatggtcggg atttagacct ttcgggaacg tttatctcaa ccaatcacgg gatttttgaa
     3541 atcgtctatt aaatctgtcg gtacattctt actggcagat tttcttgttt acggggtagt
     3601 caaaagacta tccccatttt tatgaaagga ttgattacat gaagaaaata cgaagctata
     3661 ccagtatctg gtctgttgag aaagtgttgt actccatcaa tgactttaga cttccgtttc
     3721 ccataacctt tacgcaaatg acatggtttg tcgtgtcact ctttgcagtt atgatacttg
     3781 gcaacttgcc ccctctttct atgatagagg gagcatttct caaatacttt gggattcctg
     3841 tggctttcac atggtttatg tctacaaaaa cctttgatgg taaaaagcct tatggatttt
     3901 tgaagtctgt cattgcttat gcactgcgac gaaagctgac ctatgcagga aaaaaagtaa
     3961 cgcttggcag aaaccagcca caagaagcca ttacagcagt taggagtgaa ttttatggca
     4021 tatccaatta aatatattga aaacaatctg gtctggaata aagatgggga atgttacgct
     4081 tactatgagc ttgttcccta caattactca tttctaagtc cggaacagaa aatacaagta
     4141 catgattctt tcagacagct tatcgcacaa aatcgtgatg gcaagattca tgctttacaa
     4201 atcagtacag aatccagcat actttctgca caagaacgtt ccaaaaatga agtcactgga
     4261 aagctcaaaa cggttgccta tgacaaaatc gaccaacaga cagacgcttt aatatccatg
     4321 attggcgaaa atcaagtgga ctaccgtttc tttatcggtt ttaagttgct tctcaacgat
     4381 caggagtttt ctatgaaaag tcttaccgtt gaagcaaaaa atgcttttac tgattttgtc
     4441 tatgatgtga accataagct gatgggcgat tttgttagta tgagtaatga tgaaatcctg
     4501 cgttttcaga agatggaaaa gcttttagaa aataaaatct cccgtcgttt caaaatccga
     4561 agattagata aggatgactt cggctatctg attgaacacc tttacggaca gacaggcact
     4621 gcctatgaag agtatgagta tcctctatca aagaaaaagc tggaacatga aacactgatt
     4681 aaaacctatg acctcattaa gcctactcgc tgtctggtgg aagaaaaaca gcgatatttg
     4741 aaaatccagc aggaagacga aaccgcctat gtagcttact ttaccatcaa cagtattgtc
     4801 ggcgaactgg acttcccgtc ctctgaaatc ttctactacc agcaacagca atttaccttt
     4861 ccgattgata cgtcaatgaa tgtggaaatt gtagcgaatc gtaaagccct atctactgtc
     4921 cgcaataaaa agaaagaact gaaagacttg gataaccacg cttggcaaag tgataatgaa
     4981 accagctcta atgtggcgga agctctggaa agtgtgaacg agctggaaac caatttagac
     5041 caaagcaagg aatctatgta taagctgtcc tatgttgtaa gggtatcagc aaatgacctt
     5101 gacgaactta aacgtcgttg taatgaagtg aaagatttct atgatgattt gagcgtgaaa
     5161 ctggtacgac cttttgggga tatgctgggc ttacatgaag aatttttacc tgccagcaaa
     5221 agatatatga atgactatat tcaatacgtg acctctgatt tcctcgctgg tttaggtttt
     5281 ggtgctactc aaatgctggg tgaaaatgag gggatttatg ttggctacag cttagatact
     5341 ggacgcaatg tctatctgaa acctgctctt gccagtcaag gggttaaggg ttcagtaacc
     5401 aatgcgttag cgtctgcctt tgtcggttcg ctgggtggtg gtaaatcctt tgcgaataac
     5461 cttatcgtct attatgcagt gctttatggg gcacaagcag tgattgtaga cccaaaagca
     5521 gaacgtggca gatggaaaga aaccttgcca gagatttccc atgaaatcaa tatcgtcaat
     5581 ctgacttctg atgagaaaaa caaaggctta cttgaccctt atgtaattat gaaaaatacc
     5641 aaagattctg aatcactggc tattgatatt ttgacattcc ttacggggat ttcctctcgt
     5701 gatggggaac gcttcccaat ccttagaaaa gccattcgtg cagtaaccaa tagtgaagtg
     5761 cgagggttga tgaaagtgat tgaagaatta cgggttgaga atacgccact aagtaccagc
     5821 atagccgacc atatcgaaag ttttacagac tatgactttg cccatctgct ttttagtgat
     5881 ggttatgtag agcagtctat cagccttgaa aaacaactga acattataca ggttgccgac
     5941 ttggtacttc ctgacaagga aacttccttt gaggaatata ccacaatgga gttactatcg
     6001 gtagcaatgc tgattgtcat tagtaccttt gcgttggact ttatccatac agaccgaagt
     6061 attttcaaga ttgtagactt agacgaagca tggagcttct tacaggtggc acaagggaaa
     6121 acactatcta tgaagctggt tcgtgctggt cgtgctatga acgctggggt atatttcgtg
     6181 acccaaaata cagatgacct cttagatgaa aaactgaaaa ataacctcgg cttaaaattt
     6241 gcctttcgtt ccactgacct taacgagatt aaaaaaaccc ttgccttttt tggtgtagac
     6301 ccagaggacg aaaacaatca gaagcgattg cgtgatttgg aaaacgggca atgccttatc
     6361 agtgatttat atggtcgtgt cggtgtgata cagttccacc ctgtatttga agaactgctc
     6421 catgcctttg ataccagacc acctgtgcga aaagaggtgt aaatgtgaaa ccatcaatat
     6481 taaacagaat aaaatcaaac tggacgctga aacgtctagg taaagtggca atgacagtgg
     6541 ctttcacact tgtgattgcc atttttcttt tagccatgct gggaacggtg gttcaagctg
     6601 cgggcttggt agatgatacg gtcaatgtgg caaatgaata cagccgatac ccacttgaaa
     6661 actatcaact ggatttttat gtggataata gctggggctg gctaccgtgg aactggtcgg
     6721 acggaattgg aaaacaagtc atgtatggac tatatgccat taccaatttt atttggacaa
     6781 tcagtcttta tgtttccaat gcgacgggtt acttagtaca ggaagcctat tccttagact
     6841 tcatttccgc tacagcagat tccattggca agaatatgca gacccttgca ggggttacgc
     6901 ctagtggact atcaacagag ggtttctatg ttggattcct tttactcttg attttggttc
     6961 ttggggttta tgttgcctat acaggactga taaagagaga aaccacaaag gcaattcatg
     7021 ccctcatgaa ttttgtgctg gtttttatcc tatcggcttc ctttattgcc tacgctcccg
     7081 actacattaa aaaaatcaat gacttttcat cagacatcag caacgctagt ttgtcactgg
     7141 gtacaaaaat tgtcatgccc cattcagata gtcaaggcaa ggacagtgtg gacttgatac
     7201 gtgacagcct attttccata caggttcagc aaccgtggct actgcttcaa tataacagtt
     7261 cagacattga aagtattggt attgaccgtg tggaaagcct gctctcaacc agtccagatt
     7321 ccaacaatgg ggaagacaga gaaaaaattg ttgcggaaga aattgaagat agaagcaata
     7381 ccaatatgac gattacaaag acgattaacc gtttaggtac agtcttcttc ctatttgtct
     7441 tcaatattgg gatttccata tttgtattcc tattaacagg aatcatgatt ttctcgcagg
     7501 tactttttat catctatgct atgtttctgc ctgtgagctt tattttaagc atgattccat
     7561 catttgatgg tatgtcaaaa cgagccataa caaagctctt taataccatt ttgacacgag
     7621 ctggaatcac attgattatt acgacagcat ttagtatttc aaccatgctc tataccttat
     7681 cggctggtta tccgttcttt ttgattgctt ttctacagat tgtgaccttt gcaggaatct
     7741 actttaagct gggcgattta atgagtatgt tttctctaca gagtaacgat tctcaaagtg
     7801 tgggaagtcg tatgatgaga aaacctcgta tgcttatgca cgctcacatg caccgtctac
     7861 agcggaaact tggacgctcc gtgactgctt taggggctgg gtctgccatt gccagtgcta
     7921 cgggtaaaaa aggacagtcg ggttcgggaa gttctgcgag tacacaagca gatcactctc
     7981 gaccaaacgg gcaggaaaaa tcaacactag gaaagcgtat cggtcaaacc atcggtacag
     8041 tagctgatac caaagacaga atggtagata ctgctggtaa tttgaaagaa caggttaagg
     8101 atttgccgac caatgcaaga tatgcagtat atcaaggaaa atctaaagtg acggataatg
     8161 tccatgattt aaccagaagt atttctcaaa ccaaagcgga taaagccagt ggacgcaaga
     8221 aacagcagga acaaagacgg aaaaccattg cggagcgtcg ctctgaaatg gaacaggtca
     8281 aacagaaaaa acagccagct tcttccgttc atgaaagacc agccactaag caagaacaat
     8341 ctcatgatgg acagacttca aggcaaacaa ctgtacaggc ttcctatagg gaatctcaac
     8401 aagccaaaca agagcgtcca acagttaagt ccgattcttc aagtttaaaa acggaacgtc
     8461 aaagtaatac agttcaagaa agaacggttc aaaagccagt aacttcaact gcaccaacag
     8521 atagagcttc acaacgttca atcacaaaag aacgtcagtc tactgttcaa agagtaccac
     8581 tacaaaatac aaaaagtaga ccaccaatca aaaccgccac cattaagaaa ggcagtaaga
     8641 aaccatgaag ctgaaaactt tagtgattgg tggttcggga ttattcttga tggtcttctc
     8701 actgcttctg tttgtcgcca ttttattttc agatgaacaa gatggcggtt tttccaacat
     8761 tcactatggc ggtgtggatg tttccgcaga agtgctggct cataagccta tggtagaaaa
     8821 agttgccaaa gaatatggaa ttgaagaata cgtcaatata cttcttgcga ttatacaggt
     8881 ggaatcgggt ggtacagcag aagatgttat gcagtcctcg gaatccctcg gtcttccacc
     8941 taattcattg agtacagaag aatccattaa gcaaggtgtg aagtatttca gtgagttatt
     9001 agccagtagc gaaaggcttg gtgtagattt agaatcggtt atccagtcct acaattatgg
     9061 tggtggtttc ttaggatatg tggctaatcg tgggaataaa tatacctttg aactggctca
     9121 aagtttctca aaagagtatt cgggtgacga aaaagtgtct taccccaatc ccatagccat
     9181 acctatcaat gggggctggc gatacaatta cgggaatatg ttttatgtgc aactggtaac
     9241 gcagtatctt gtcacaacag agtttgatga tgatacagta caagccatca tggacgaagc
     9301 tctgaaattt gaaggctgga agtatgtcta tggcggagct tccccgacta cctcttttga
     9361 ttgtagcgga ctgacacaat ggacgtatgg aaaagctgga atcaacttac cacgaacagc
     9421 acaacagcaa tatgatgtaa cccggcatat cccattattg gaagcaaaag ccggcgattt
     9481 ggttttcttt cattctacct ataacgctgg ctcttatatt actcatatcg ggatttattt
     9541 aggcgataac cgtatgtttc atgcaggcga ccctatcggt tatgccgact taacaagctc
     9601 ttactggcaa cagcatttag tgggagcagg acgaatcaaa caataagaaa ggacgattca
     9661 atgatgaaat tcggaaaaaa tcagaataca gaaaaacaga caccaaagga aaagaaacct
     9721 cgtgtctata aggtcaatcc tcgtaaaaag gttgtgattg tcctatgggt acttttagga
     9781 cttagtttca gctttgcgat attcaagcac tttacagcca ttgataccca taccattcat
     9841 gaaaccacca tcatagaaaa ggaatatgtc gatactcatc atgtggaaaa ttttgtagag
     9901 aactttgcga aagtctacta ttcatgggaa ctaaacgaca agtccattga taatcgcata
     9961 gaaaacctga aagcatatct gacagaggaa cttcaagctc tgaatgtcga tacagttcgt
    10021 aaagatattc ctgtatcgtc ttctgtaaaa ggatttcaga tatggacggt agaagcagat
    10081 ggcgacaatc aatttgatgt aacctacagt gtagaccaac tcattacaga gggggaaaat
    10141 acaaagaccg tccactctgc ttatatagtg agtgtctatg tagacagtac tggaaatatg
    10201 gtcctcatta agaatccgac cattactagc ataccaaaga aatcagacta taaaccaaaa
    10261 gctattgaaa gtgagggtac ggttgattcc attacaacca atgaaatcaa tgagttttta
    10321 acgacgttct tcaagctcta tcctacagcg acagccagtg aactttccta ctatgtgaat
    10381 gacgggatat taaaaccaat cggaaaagag tacatctttc aagaactggt aaatcctatt
    10441 tacaatcgta aggataatca agtcacggta tcgctgacag tggagtatat cgaccagcag
    10501 accaaagcaa cgcaggtatc tcaatttgat ttggtacttg aaaagaacgg gagtaattgg
    10561 aagattatag aataacaaat attggtacat tattacagct attttgtaat cacgtactct
//
LOCUS       DAGCWU010000100        10564 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.100, whole genome shotgun sequence.
ACCESSION   DAGCWU010000100 DAGCWU010000000
VERSION     DAGCWU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.100"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            12..84
                     /locus_tag="MBZ71_001915"
     tRNA            12..84
                     /locus_tag="MBZ71_001915"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="MBZ71_001916"
     tRNA            88..160
                     /locus_tag="MBZ71_001916"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="MBZ71_001917"
     tRNA            168..249
                     /locus_tag="MBZ71_001917"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="MBZ71_001918"
     tRNA            267..339
                     /locus_tag="MBZ71_001918"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="MBZ71_001919"
     tRNA            351..422
                     /locus_tag="MBZ71_001919"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="MBZ71_001920"
     tRNA            439..524
                     /locus_tag="MBZ71_001920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="MBZ71_001921"
     tRNA            535..608
                     /locus_tag="MBZ71_001921"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="MBZ71_001922"
     tRNA            618..691
                     /locus_tag="MBZ71_001922"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="MBZ71_001923"
     tRNA            711..783
                     /locus_tag="MBZ71_001923"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="MBZ71_001924"
     tRNA            811..884
                     /locus_tag="MBZ71_001924"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="MBZ71_001925"
     tRNA            898..971
                     /locus_tag="MBZ71_001925"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="MBZ71_001926"
     tRNA            995..1084
                     /locus_tag="MBZ71_001926"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="MBZ71_001927"
     tRNA            1097..1170
                     /locus_tag="MBZ71_001927"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="MBZ71_001928"
     tRNA            1189..1264
                     /locus_tag="MBZ71_001928"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="MBZ71_001929"
     tRNA            1266..1336
                     /locus_tag="MBZ71_001929"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="MBZ71_001930"
     tRNA            1350..1423
                     /locus_tag="MBZ71_001930"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="MBZ71_001931"
     tRNA            1448..1535
                     /locus_tag="MBZ71_001931"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="MBZ71_001932"
     tRNA            1557..1628
                     /locus_tag="MBZ71_001932"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="MBZ71_001933"
     tRNA            1651..1740
                     /locus_tag="MBZ71_001933"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="MBZ71_001934"
     tRNA            1753..1826
                     /locus_tag="MBZ71_001934"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="MBZ71_001935"
     tRNA            1845..1917
                     /locus_tag="MBZ71_001935"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="MBZ71_001936"
     tRNA            1921..1993
                     /locus_tag="MBZ71_001936"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="MBZ71_001937"
     tRNA            1998..2068
                     /locus_tag="MBZ71_001937"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="MBZ71_001938"
     tRNA            2081..2154
                     /locus_tag="MBZ71_001938"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="MBZ71_001939"
     tRNA            2166..2237
                     /locus_tag="MBZ71_001939"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="MBZ71_001940"
     CDS             2503..3696
                     /locus_tag="MBZ71_001940"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBT4429866.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="MBZ71_001941"
     CDS             3826..5298
                     /locus_tag="MBZ71_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4429867.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="MBZ71_001942"
     CDS             5381..6169
                     /locus_tag="MBZ71_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBT4429868.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="MBZ71_001943"
     CDS             complement(6236..6904)
                     /locus_tag="MBZ71_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBT4429869.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="MBZ71_001944"
     CDS             7019..8731
                     /locus_tag="MBZ71_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429870.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="MBZ71_001945"
     CDS             8734..10506
                     /locus_tag="MBZ71_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429871.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3029 a         1925 c         2449 g         3161 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 tttt
//
LOCUS       DAGCWU010000101        10322 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.101, whole genome shotgun sequence.
ACCESSION   DAGCWU010000101 DAGCWU010000000
VERSION     DAGCWU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.101"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            59..832
                     /gene="sufC"
                     /locus_tag="MBZ71_001946"
     CDS             59..832
                     /gene="sufC"
                     /locus_tag="MBZ71_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBT4429872.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            845..2131
                     /gene="sufD"
                     /locus_tag="MBZ71_001947"
     CDS             845..2131
                     /gene="sufD"
                     /locus_tag="MBZ71_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBT4429873.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            2128..3363
                     /locus_tag="MBZ71_001948"
     CDS             2128..3363
                     /locus_tag="MBZ71_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBT4429874.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            3350..3820
                     /locus_tag="MBZ71_001949"
     CDS             3350..3820
                     /locus_tag="MBZ71_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBT4429875.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            3825..5216
                     /gene="sufB"
                     /locus_tag="MBZ71_001950"
     CDS             3825..5216
                     /gene="sufB"
                     /locus_tag="MBZ71_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBT4429876.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5311..6453)
                     /locus_tag="MBZ71_001951"
     CDS             complement(5311..6453)
                     /locus_tag="MBZ71_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4429877.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(6492..7226)
                     /locus_tag="MBZ71_001952"
     CDS             complement(6492..7226)
                     /locus_tag="MBZ71_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4429878.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            7458..7733
                     /locus_tag="MBZ71_001953"
     CDS             7458..7733
                     /locus_tag="MBZ71_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4429879.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7831..9828
                     /locus_tag="MBZ71_001954"
     CDS             7831..9828
                     /locus_tag="MBZ71_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429880.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9904..10239)
                     /locus_tag="MBZ71_001955"
     CDS             complement(9904..10239)
                     /locus_tag="MBZ71_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4429881.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
BASE COUNT         3367 a         1815 c         2082 g         3058 t
ORIGIN      
        1 ttcattctca attagtctaa aatgagtgag aacgaaagag aatttggggg tataatgcat
       61 gtctgtctta gaaataaaaa atctacatgt gtcgatcgag gaaaaagaaa tccttaaagg
      121 cgtaaaccta acaatgaaaa ccggagaaat ccatgccatc atgggaccta acggcacagg
      181 aaaatctacc ttatctgccg ctatcatggg caatccaaac tatgaagtaa cagaaggcga
      241 aattttgttt gacgggaaaa atgttttaga cttggaagta gatgaacgtg cgcgacttgg
      301 cttattttta gctatgcaat atccaagtga gataccaggt atcacgaatg cggaattcat
      361 gcgtgcagcg atcaatgcga aacgtgatga aaacgacaaa atttctgtta tggcattttt
      421 gaaaaagtta gatgaaaaaa tggctttact aaatatgcca gaagaaatgg ctgaacgtta
      481 tctgaacgaa ggtttttctg gcggagaaaa gaaacggaat gagatcctgc aattgttgat
      541 gttagaacct acatttgcaa ttttagacga aatcgattca ggtctggata tcgatgcttt
      601 gaaagtcgtt gctaaaggag taaatgaaat gcgcggagac aatttcggtg cattgatcat
      661 cacccactat caacgattat tgaactatat cacacctgat gtcgttcaca tcatgatgga
      721 aggccgagta gtcatgacag ggaatgctga cctagctaaa cgtctggaag cagaaggcta
      781 cgctggtatc agtaaagaat taggaatcga ctataaagaa gaagaagcgt aagggggagc
      841 aagaatgaaa gaaaaaaact ggctcgatta tcttgacgcg gtaaatgatt tttctttaag
      901 taaaggcgaa cctgattgga tgagaacctt ccgtcaagac gcactagcga aagcagatga
      961 actgccattg ccgcatatcg atcgggtgaa attccatcgc tggtcgttat ttgatgtgaa
     1021 agaaacacaa accatcagtg aaacaggcac gattccggca tttgatgcaa tgaaagacaa
     1081 tcctgtgctt gtccaacaag gttcatggac gatttttgaa caactgcctg tggaattagc
     1141 agaaaaagga gtcattttta ccgacttgtt cactgcaatg atcgaatatc cagaactagt
     1201 ccaagaatat tatatgaaaa aagcagtaaa catgaacgaa gaccaattga ctgcactcca
     1261 tgtagctttt atgaacagcg gtattttcct gtatgtgcct aaaaatgtcg tgatcgatga
     1321 accactcgag tccttattca ttcaagatgg cgcatccgat gagcacttct tcaagcatgt
     1381 gttgatcgtg gcagatgaac atagtgaatt ttcttatctg gaacgtttcc agacaaccaa
     1441 agaacaagta gcaaaatcat caggtaatat catcgtggaa gtcatcgcta aagctggttc
     1501 aaaaatcaaa tattcagccg tagatcaatt aggagaaaat attacttctt atatgaatcg
     1561 ccgtgggcac attttacgag atgcttctgt cgactgggca attggtgtca tgaatgacgg
     1621 tcatgtgatc gctgactttg actcagattt agcaggagaa ggtgcacacg cagaagtcaa
     1681 aatcgtggca atcagctcgg gcagacaaat ccaaggaatc gatacacgag taaccaataa
     1741 agcccctcat acgatcgggc atattttgca acacggagtg atccgagaaa aaggaacatt
     1801 gacctttaat ggtatcggcc acatcttgaa aggggcaaaa ggtgcagatg cccaacaaga
     1861 aagtcgtgtc ttgatgcttt ctgataaagc acgtggtgat gcgaatccta ttcttttgat
     1921 cgatgaaaac gaagtaactg ctggacatgc tgccagtgta ggacgagttg atccagaaga
     1981 aatgtactat ctgatgagta gaggattgca taaagaagaa gcagagcgct tagtgattcg
     2041 aggattcctc ggttctgtct taacagcaat ccctgtagaa caagttcgga aagaattagt
     2101 cgaagtaatc gaagggaagt tgaatggatg atcgatgcta aaaagctacg caatgatttt
     2161 cctatcttat ttcaagacgt caatgatgaa cctttagttt atctggataa tgcagcgacg
     2221 acccaaaagc caaaacaagt actccaggca ttagcgtact attacgaaca cgataacgcc
     2281 aatgttcatc gcggtgtcca tacgttagca gaacgcgcga cccatgatta tgaacaagca
     2341 agagaaaaag tacgggcgtt catccatgca aaagaaacag cagaagtgct gtttactcgt
     2401 ggtacgacga caagcttgaa ttgggtagcc agaaccttcg gcgaccgcta tatcaaagca
     2461 ggagacgaga tagtgctctc ttacatggaa catcattcta atgttatccc gtggcagcag
     2521 ttggcaaaac gaacaggtgc tgtcttgaaa tatatcgaac taacagaaga aggcttcctg
     2581 gatatggagg atgcagcgaa aaagattacg gacaaaacca aaatcgtctc cattgctcat
     2641 gtatccaatg tcttaggtgt cttcaatcct gttcaagaat tagctgaact tgtccatgaa
     2701 aacggcgggg tcatcgtagt agatggtgct caatctgccc cgcacatggc cattgatgtc
     2761 caagcactag actgtgattt ctttgctttc agcggacaca aaatgtgcgg accaacaggt
     2821 attggtgttt tatatggtaa acgaaaatgg ttagaagaaa tggaacctgt tgaatttggt
     2881 ggcgaaatga tcgattttgt cgaattatac gacagtacat ggaaagaact accttggaaa
     2941 ttcgaagcag ggacaccaaa tatcgccggc gctatcgcat taggccatgc aatcgattat
     3001 ttgcaaaaac tcggtatgga caatatccat cgatatgaag aagaactagc cgcttatgtg
     3061 ttgccaaaat tacaagcaat tgatggattg actgtttatg gtccacaaga cccgaatttt
     3121 cggacaggag tcgttgcatt caatttggat gggttacatc cccatgatgt cgcgacagct
     3181 ttagacatgg aaggggtagc cgtcagagca ggtcatcatt gtgcgcaacc gctattgaag
     3241 taccttgaag tatcagcgac tgctcgcgcg agcttttatt tctacaacac aacgcaagac
     3301 gccgaccgtt tagttgaagc aatcatggca acaaaggagt ttttccaaca tgggactatc
     3361 taaattagac aatttatacc gtcaagtgat cttggatcat tcttcccatc cgcatcatcg
     3421 tggaagcttg actgactcga caagcaagat cgaattgaac aaccccacat gtggagatgt
     3481 gatcgaattg caagtcgatg taaaagacgg aatcatcaaa aatatcgcct ttgacggcag
     3541 tggctgctcc atcagtacgg caagtgcctc gatgatgaca gacgccgtca tcggaaaatc
     3601 aatagaagaa gcagaacatt taactgtaga ctttctacag cttgtccaag gaaaagaagt
     3661 caaagatccc gaccaattag gcgatgctgc tatgcttggt ggtgtagcta aatttcccgc
     3721 tcggatcaaa tgtgcgacac ttgcctggaa agcactagga aaagcaatgg aaaacgatgg
     3781 taccgcagct ttccatcatt accacaagga agaggagtga gaacatgggc gtaccagaat
     3841 tagaagaata taaattcggg tttcacgatg atgtcgaacc ggttttcagt acaggagaag
     3901 gcttgacaga agaagtgatc cgtgagatgt caagaatcaa aggcgaacca gaatggatgc
     3961 ttgaattccg tctgaaatct ctagaaacat ttaacaaaat gccaatgcaa acatggggcc
     4021 ccgacctttc agacatcgat ttcgatgcca tcaaatatta ccaaaaacca agtgacaaac
     4081 ccgctcgtga ctgggaagac gtaccagaaa aaatcaaaga tacatttgaa aagattggta
     4141 taccagaagc tgaacgagct tatctagccg gtgcttccgc acaatatgaa tcagaagtcg
     4201 tgtaccataa tatgaaagat gaattcgaaa agttaggaat cgtctttact gacactgact
     4261 ctgctttgaa agaataccct gaattattca aagagtactt ttcaaaactt gtaccgccaa
     4321 ccgacaacaa attagcagcg ctgaattcag ccgtatggtc aggtgggacc tttatttacg
     4381 tgccaaaagg cgtcaaagtc gatgttccat tacaaacgta cttccggatc aatgcggaaa
     4441 acacggggca attcgaacgt acgctgatca ttgttgacga aggagcaagc atccactatg
     4501 tcgaaggttg tacggcacct acttattcaa gtaacagcct gcatgccgcc atcgtagaaa
     4561 tctttacaag aaaagatgcg tattgccgtt acacaacgat ccaaaactgg tcagacaatg
     4621 tgtacaactt agtaaccaaa cgagcaaaag catacgaagg cgcaaccgtt gaatggatcg
     4681 acggaaacct aggtgcaaaa acaacgatga aatacccaag tgtttactta gatggaaaag
     4741 gtgcacgagg caccatgctc tccatcgcct ttgcaggagc aaaccaaatc caagatacgg
     4801 gtgcaaaaat gatccacaat gcaccaaata catctagctc tatcgtctct aaatcaattt
     4861 caaaagacgg cggagaagta aactatcgag gacaagtaac ctttgccaaa aacagtgcgg
     4921 gttcgatctc tcatatcgaa tgtgacacga tcatcatgga cgaatggtcg aaatccgata
     4981 cgatcccatt caatgagatc cacaacagcc aagtctcttt agaacacgaa gccaaagtct
     5041 caaaaatctc agaagaacaa ctctactatc tgatgagccg aggcctgacc gaatctgaag
     5101 caactgaaat gatcgtcatg ggctttgttg aaccattcac caaagaactt ccaatggaat
     5161 atgcagtcga actcaatcga ttgatcagtt atgagatgga aggcagtgtt gggtaaacct
     5221 gttataggag ctagaaccct tgatatataa gggttctagc ttttttgttt tgcctgtata
     5281 ctagaaatga ccagattttg accagctctt ttactgatga gccatcatat cagcaaagtc
     5341 ttgacctgtt ttcttaggtg cattatttgt aatatgtgca tagatgtcta atgtcacttt
     5401 actattttca tgccctaatc gttgttgaac taccttgaca gattctcctg cttctaataa
     5461 taaactagca tgtgtatgtc taaacccatg aggtgtaata cgaggcaagt tatatttttt
     5521 caaaatatat cttaaccaat cattaacagt gtttggaatg tatagagtgt ttttaactgt
     5581 tgtaaaaaca tgttgctctt tagagctagt attgtatcca agttttaaat actcttcttt
     5641 ttgctgtaat ttccagttgt tgaggataga aagagtctca gtatctaaag agataactcg
     5701 tctagaagct cttgttttcg gagattgaat tataacttta ccaaattcat ctacagcaag
     5761 agttttattg acatgtagtt ctttattaaa cgtgtctatg tctttccact gtaaagcgag
     5821 tacttcactc tttctcatac ctgtgtatgc caataatctg aagaaggcgt attgtttcat
     5881 gttaccaaaa tctttaaaag catccagtaa attattaagc tcctcttttg agtaaaattt
     5941 acgatttttc tcatactctt tttttcgtgg aagaagtgtt ttttccatag gattattgtc
     6001 aatatattgc attgcaaccc cgtagcgtaa aatttgtgca gtctgttttc tcatataatc
     6061 gtaactttca tatttactgt gccattcatc taccactttt tgacaatatg ggacagagat
     6121 attcgatagt ttaagcttac caaatgctgg aagtatttga ttatttgcgt atcttactga
     6181 gattgcgatt gttgaagctt taacactggt acgctgatga gttatccatt cattatacag
     6241 gtcttcaaat gtcatatcag gtgctttctt tttaacaccg tataatattt gatgtctgta
     6301 ctcgtcataa tctttttgag cttcttgttt gctactaaaa cctcttctcg tagtatttat
     6361 tttttcacca gttcgtggat cggtggcaat gtgtccttta tacatccacg ccacttgacc
     6421 gtttttcttt ttgtactttg atatttttgc catgaaatca tctcctctta gtttacaagg
     6481 agtacttacc actagatggt atcacctccc ttcatttttt tcttagtgta ttttttccca
     6541 attgatgaaa tcgtttgttc tagcttgttt atattatcag taatttctct cacagcttct
     6601 tcgggataag acatgtcttt caattgaata ggtagccatt catctaccat tctttctagt
     6661 agttcttcta tactgctagt gaggaaaaga atgttattgt caaattcttc tacaatagca
     6721 ttgatgaata attcttctat ttttaagata ttgaaaagac taattttttc atttttaact
     6781 ttattacaag tcttgttagc aataaattct tgcatctctg gttctaattt ggaatatcta
     6841 tccagccatg tatctaaatt agagtgtact ggtgggaact gttgacgaag atgctggaac
     6901 aggtttttat ctggaaatgt tataccatct agtacaatgc tatctctata aagaataatt
     6961 cccaaaatat attcttctac attgccacaa actaactcct ctatactcat atttcccaaa
     7021 tcagcaattc gggataatcg ctctggtatt ggtaaatagg ttcccttctc ccattcgtag
     7081 atagtctttt tgtcaattaa ttgaccgttg ttaatcataa gaccaaatgc tggttgtgtt
     7141 aattttttac tctttcttat agcttttatt tttgttccga gtatcttttt tcgttctaaa
     7201 taatttattg gtggacgttt tatcatattg accacacctc ctaaaattag tatacctcaa
     7261 aaaaggggtg ttgtaaataa attttacaaa actattgaca cccctttttt atcgaggtat
     7321 tatgtgtatg tcgatgaact ggtaatcatt ttttgtttga ttaatcggtt aataacaaaa
     7381 tacttttatt ttgagcaaaa aatacgaaga taattcttag aaaatttgtt caagcaatca
     7441 cgaaaggagg gggaaatatg tctctacaaa tcattgatag cattgtttct atacaagttg
     7501 aacaacaagt gaaagagata attacagtgc taaagcctat gctagaagac attatttctg
     7561 gagtcactct agagtatatg gactatcagc aggcaagtga atatctaggt gtatcagtag
     7621 gaacgattag aaaatatgtt tctcaatacg ggttacctgt tataaaaatt gatacggtca
     7681 ttagatttaa aaaatctgat attgatagtt tcatggaacg gtataaaggt tgagtaaaca
     7741 aataagacta aaacatatag taataaagag tttgttttta ttatctgtat gttttagtct
     7801 ttaacagaat tttataagga ggaaacacaa atggaaacat caatgattaa tattccaatt
     7861 tatgtagatc aagaaaaatt attagagaag ccagttacaa aaaaagaaaa aactgatgta
     7921 agagataggt gtttagataa ctatagagaa gttacaagag atgagtttgt agagatacta
     7981 aattcatcac aatcatttat tccaagcaag acaaagaata aaggcaatga caaggagagt
     8041 tttgtggaaa ccagagtaat tattctggat gtcgataaca ctgttaaaga tgagaagggg
     8101 aaggtagtag atttgagtaa agatgatagt agatacttaa gcatagaaaa ggttttatct
     8161 atcaatttag ttcataattc tgcgtttgcg atacaaaaaa gtattcggta ctctgaaaat
     8221 ctagaaaaat acaagattgt ctttctatta aaagaagcaa ttactgacta tggtaaaatg
     8281 gttgctgttt acgaatactt gaaaaagaac atccccgggt gtgatgacaa tgtaaatgct
     8341 tcgaatagaa tgttttttgg aggatataag tcagatgcag tgataataat taatgaagac
     8401 aacatgttag atattactga gcttccgatt gattttacca tgacatgttc taaaaattgt
     8461 caaagtgatt cagggagaga gtatgaacta gtcaatgaaa ccgattttgt taagttgata
     8521 aaaaatgacg acaaagaaga aatgaaacag tggtttaaag actgcctgtt tgattcctcc
     8581 gacatgtctt ttaatgagat ttatgaaaga ttgttaacca ttgatatgaa taaattactt
     8641 aagtcaagta aaaacttaag atgtctgttt cacaacgatc ataatccctc tgctagtatt
     8701 tttaccagta aaactgggca ttccattttt tattgtcatt ccagtaattg tgatatcagt
     8761 gaaaatttta ttggagttgt gatgctaatt ttaaagaaag acagtcgggt agaaacattt
     8821 aattggctat tagagaacct agacttgtat cctacacatt ttcgaaaatt gaaagaggaa
     8881 tcaaaagtac tttttgacac tcttgaatca atgaaagaca aacttttcag gacaataagg
     8941 aattatctag aggaaatgcg acgtgtctat gatgtcttgc tttctgatgt taattttttt
     9001 gatgatagta aggaaagtgt gacttgtatt ttgtcggggg aacaattagc taaaaaaatg
     9061 agttcttact atggtaaaac ttatgacata gataaatgta ataagttact atcacttatg
     9121 acttttatag gattgataaa aaaattagat gatgaagatg ttcctttgaa gatgctcgaa
     9181 tacctaaata ggttaaagga agagaaagcg ttaggaaact ttaatcataa gagaagcaat
     9241 gtctatcgat tagacgtttt taatgcaact tctgtgattc aaaaattgaa tgaagatatc
     9301 cttccacttc tggaaaaaat tcattttaca tatcaatatt tttcttatga atgggttaag
     9361 atatgtttta acgaaaaaga ggctaaaaga gtatttcctc aaaattcccg atcagaactc
     9421 agcaaggaaa aacaattgat tttagatgaa gcacatgaag ttttacaggg cttatcgtgg
     9481 caagggacat atctaatgac agagaaagaa cttattaaca ctgtttgtga acgcctacgt
     9541 aatgttgggg ataacagcgt gaaaaatgct ttaataaaaa acagaggcta ctttgttaaa
     9601 caaggattca ctcttgaacg agcttctaag gatattaaga aactatacaa tgtgaaagca
     9661 agtagtagtt ttttaatata tttagcgaaa ggggcaggga tagatgaaac aataaaagaa
     9721 ttgatattat caggagctgg gatagttcct ctgtatcaat ttacaagtgt aacaagagat
     9781 gatggacgga gagtaaggaa gagaaaagac ccaattgtat taaactagac aggttatcca
     9841 tagctcttaa tcttatttca tctcaataca cccaccgtta aagttcggtg ggttatttat
     9901 ttttcaatct ttcaatagct gattgcgttc attttcttgc ctttttaata gcttcatata
     9961 gtcaattgtt cgaactgcat cttcagtgct aagagaacga tagagagcca aaaactcttc
    10021 ctcataatct acgacaacat tgtctagaaa atggcttaca ggggttccta gggctctaga
    10081 aagagctgtt aagctatcga ggttaggctc tgttacagaa ttttcccatc ttgctactgc
    10141 ctgttgtgta gagaagattt tttcagctaa ttctttttgt gtccacccga gagcttttcg
    10201 cttttctcta atgacctttg catattgttt ctgtttcatt aatacacccc catctcagta
    10261 tagtacaaca agttgttgta tttgtaatgg tatttgaagt tttttatatt ttatgagttg
    10321 aa
//
LOCUS       DAGCWU010000102        10218 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.102, whole genome shotgun sequence.
ACCESSION   DAGCWU010000102 DAGCWU010000000
VERSION     DAGCWU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.102"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(259..411)
                     /locus_tag="MBZ71_001956"
     CDS             complement(259..411)
                     /locus_tag="MBZ71_001956"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBT4429882.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(602..1480)
                     /locus_tag="MBZ71_001957"
     CDS             complement(602..1480)
                     /locus_tag="MBZ71_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4429883.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1519..2637)
                     /locus_tag="MBZ71_001958"
     CDS             complement(1519..2637)
                     /locus_tag="MBZ71_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBT4429884.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2634..4733)
                     /locus_tag="MBZ71_001959"
     CDS             complement(2634..4733)
                     /locus_tag="MBZ71_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBT4429885.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4784..5605)
                     /locus_tag="MBZ71_001960"
     CDS             complement(4784..5605)
                     /locus_tag="MBZ71_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4429886.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5602..6411)
                     /locus_tag="MBZ71_001961"
     CDS             complement(5602..6411)
                     /locus_tag="MBZ71_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4429887.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6432..6926)
                     /locus_tag="MBZ71_001962"
     CDS             complement(6432..6926)
                     /locus_tag="MBZ71_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4429888.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6938..7426)
                     /locus_tag="MBZ71_001963"
     CDS             complement(6938..7426)
                     /locus_tag="MBZ71_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4429889.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7503..10199)
                     /locus_tag="MBZ71_001964"
     CDS             complement(7503..10199)
                     /locus_tag="MBZ71_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4429890.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3127 a         2281 c         1677 g         3133 t
ORIGIN      
        1 aaaaatagaa gtgacaaaat tatcaatcat ttcaaggtaa tataactacc tgagtttatt
       61 atcgattaaa atgctaacta catttgcaag agaagcgaga gttaaaggta aatatagaat
      121 ttctactttt ttagcaatag aaataggaat aaaatattta aaatgtatag aaactttctt
      181 ttttttcttg ctatcttttt ataatcacaa ctctctataa tagaattacg atttatttta
      241 tacggctgaa aaaatctatc accaaaaaaa ttcaaacatt aagccaagga ttatgaagtc
      301 aacatttcga aaaatgaaca tacattggta agcaactata actattacca cagacataaa
      361 gtaacacaat atattactat ttaagtgtac gccaccagtg aatatcctca aggtatacag
      421 cgcacaacaa aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat ttaatgaaaa
      481 caacacgatt tgacttagag cctttttatt tgaattcatt ttcgctttat ccaacttcat
      541 ttgatcattt gattactcgg ttaaatactt ttgacatgag tatcaatgaa acattcatta
      601 attatggttg cttatttctt accgcttttc tggccaatgc taatccgccg attattccgg
      661 cattatcacc taattcgggc gtaataatgt agctatcaat tggtggtatt tcaacatatc
      721 catttataag ctcaacaaat ttatcctgaa cttttttctt catgtgttct tgtttcatta
      781 caccaccgcc aaaaataatt ctatctggcg aaaacatcag tgttgtattg tatgcacatt
      841 gggcaaggta aaaggcttca atctgccaaa acgaatcgtc agccaataaa ttctggcctt
      901 ttacacccaa ccgtttttca atcgctggtc ctgctgccat cccctctaaa caatcttggt
      961 ggaagggaca gtttcctgaa aaattgtcct ttggatgtcg cttgatcaac atatgcccca
     1021 tttccggatg actgaaccct tccacgaaag tcccattgaa caaggcacca cctccgatac
     1081 ctgttcccac agtgaagtat gcaacacctg ctaacccttt gccagaacca gcaacatatt
     1141 caccatagca agccgcattg acatctgttg tccattcaat cggaatattg aaatattgct
     1201 ttagcgttcc tacaaaattg aaattttgcc aagcaagttt tggtgttgat gtgatatgac
     1261 cgaaactcgt tgatttgcat tgaatatcaa ttggaccaaa gcagccgacc ccgatgcctg
     1321 ccaattgttc cttaaaaggt ttaaaaaaat caatgacctg cttcattgtt tcaactggag
     1381 ttgttgtggg aaaactgact ctttccaaaa tcgttaattc ctcagtaccg actgcacaga
     1441 caaatttagt ccctccagct tcaattgccc catatatcat cataggttcc tcccttcgaa
     1501 tatgtgaaaa acacttcatt aacgcggcaa gcgttctcga tcagtgaaat gacgtttagt
     1561 atttcctgaa gcttctgttg aataccagta agcgacacta gcaacatcgt cttgccgctc
     1621 aaataagcga acatcatcgt tgccgatttg ttgtaaagtg accgataaat cttcggtaaa
     1681 acagatgggg tcttgaagat gccaacgata aagaccatgt cgtggcattg cgtcgtcacc
     1741 aaaggcatga acggcattca attttcccgt ttcaaaacga tctcttgtgg catctctatt
     1801 tgattggtaa ggatacccta aaaaggctgt tgaatagttt ttcgcatgtg gacgtccgaa
     1861 ctcatcgtac tcatgaaagg cccacgcccc gccaaaataa tcttctgaac ctgttgaggt
     1921 tacggtcgga tactcactat ccccatcaat gtagaattta aactcgcctt cgccccacca
     1981 ataacgttct aggcaggtaa gcgcaaggta agtacccacg taatagcctt gaccctttaa
     2041 tcgatcaatt aacttataat cctcctgtaa tttggtaatg cgttcccgat gccaatttgc
     2101 gtgaaaatat aggctatttt cttccaattc gtccaccaaa gcgtaattta tcgtataaaa
     2161 aaatgaggac atatccttcg gatgtaaatt ctcgatcgta attttagcgg atgtttggaa
     2221 aggcatttcg aaataggaat taaagccccc tgtaggatta accactatcg gcaacgagtt
     2281 cacctcgcaa cgctctccaa acccattaca aaagaaatcc cctaaaggac tttcaacagc
     2341 agggctatcc gacccatccc aataaattct taagattaaa tcacgtaaaa caaaactgcc
     2401 aaattctgtt cgatcacgaa tcgtcatcca catatgacga attattcccg ttccagtaat
     2461 atccgcaatc acagtttctt caccagaagt cagcggaatt gcgccacgac ctttgcggct
     2521 tggtcctagc tcgcttggca acatcgcagc tttacctttc tcgcctgttg gattttcggg
     2581 agtgattgct cgactctgga tttttttaaa ttgggtaata ttttttagta tactcatctt
     2641 tttctccttt gaatgtgaat cgtttttccc ttttctacgt gaagaatttt tttcgtatca
     2701 cttattttca atggcttttc tgacaaattt gaaatactca actcggattg cgtacattca
     2761 atcgaaatga aggcttgacc gaacttcaaa ttcttccaag aaaacgttaa attatcaaca
     2821 ggatcaattt gcaatactcc ttttttgaat ttcaatccga aatattgact gatcattagg
     2881 ctgacaaaaa gacccgatgc ccagccgcat ttgccgcagc caaagtctcg cacgacttct
     2941 ccttgttgag cctctaagcg atacggccac caccaccacg aaccgtctaa atctgccaaa
     3001 caaattagtt cttctaatcg ggttacgaaa gcttgagggt tgtgaagctc gctcattaaa
     3061 acagtgataa agccaggaaa agtggcgccg gattctaatc cccaagtgat gcctttgatt
     3121 tcctcgctat aggttggatt gaaagaacta gcagaaaagg ccatggtgtt ctgatataaa
     3181 tgatctttat tatctaaaaa ttgataaaaa ggcatcaacg tagtgtctga ttcttctcca
     3241 tcatgcatca ataaattaat ttttccatcc acaatcacat cacttaaaaa gattaaccct
     3301 tgctcaagaa tcggtttttg atagtgcttc actgaatagg ttgttttctc ctcatcaccg
     3361 attccctcca aaaattgctc accaaaagga ccatcgatag tcatttgttt acaaattgat
     3421 tcctttagtt tacaggcaat gtttttgtat cgcttgctaa gactcagttg ttctagggct
     3481 tcaaaaattt ctgcaagtcc aatacaggcc ttccaaagac aaagattgct gccggtatga
     3541 tactttccaa gtgcaaaggc gtctgataac caaacagatg gaaaaagcat tggttcccct
     3601 gaccgacgtt gagctaatat gtcctcaata attcttgaag cattcagtag aacctgcgga
     3661 caccgctcaa agaattttgt atccccagta tgttcaaaat aaagactagc cagtaacgca
     3721 ctggataacg aattactcag agaatgattg atacctcctg taaattttga accaggaaac
     3781 ttaacgccat attgatcaaa ccatagaacc gttttttgac ataactccgg ttcaaacatg
     3841 gtgaatggca atgatgcgta atacatatcc ttattccaaa ttcgattagt agcaggatag
     3901 ctgccccaat tgctgccgac gacctgtccc tgtcgattca taccgaaact agaaaagctt
     3961 tgataaagtg cacgacgata gagatggaca actctatcgt ttggcattga cagctgccca
     4021 aatagctgag caaaatagtt caatgtttcc ttcagccatt tttcgatatc agattcctga
     4081 aaagtcatta cttcttcaaa agcatttggg tccacaaaag caatggcgaa gcttgccact
     4141 tcctgttgtg cgatcgtgtc cttatttcct ttttgagaaa tcaacacatt ttgctgatcc
     4201 gaatacttct cttgatacga aggcacatct ttcactgaaa cttgaaggct ttcgtgtgtt
     4261 tcattcacaa tgtaaacctg ttgaacaagc atacttaaac gcttattagc cagtatggga
     4321 gcaaaggata ctacaatgac cttaaacgtc gaataatgat gaatatatct aggcaaacag
     4381 tcttgaatca catcgacttc aatacgatga tccgattctg acaaatggta gacaatcccg
     4441 tttacttcaa cctttaagga taacgaattg gtttgagaga gctgtttatt gacccaaaca
     4501 cctggttttt cctcagttag taaattttct cgataaaagg tcaacgtttg aatcgtcccg
     4561 caaacatcaa ggtttgctaa tgtcttttga ttacttacat cgaaggtcaa tccctttaca
     4621 tcaggaatat tccgatagta gctgtattcg tttgaagtag tcacatttcc gtcgtttaac
     4681 tcagcattaa aaaatgggtg tttgggtgaa taaattactt cattaatagt catcttttcc
     4741 ctccttgcta aaaaaactgc agcctagtag ctgcagcttc ctactatgcg attccaagta
     4801 atgacaataa aattcccaag acgaccattc caaaaataat tttgatgacg ccgacttttt
     4861 tatctagtaa tttgaaacag ctcaatgtta ccaataacgg tacaattcct ttaaaaatac
     4921 tatcgaggat ttcttggaca ttcattagtt cttcgccctt cattgaaaag ctcaaaacgg
     4981 tattgaattt gaccatactg atcgtcattg cacctaccat gatcagaccc aagatactag
     5041 cagcttttgt cagaatttca attaacccgt tcgcatacag cttttcaata tatttagagc
     5101 ctaatgaata gcctaaaacg ccgccatagt atcgcatcaa gtaattaggt atattgaaca
     5161 taactaaaaa gataattggt gccaaaatat ttccggcatt ggctaagttg atcgctagtc
     5221 ccgcggtaac gacacgccaa actccccaga acaatgaatc gccaattcca gccaatggtc
     5281 ccatcaaact cgttttgatc gcgttgatac tttccacgtc aaaaccagct ttttcagaat
     5341 tttccttctc catcgatgcc gcaatcccca tgacaaagga cgcgcaagga atggtcgtcg
     5401 aaaaatactg tgtatgacgt tccagcgccg cagctcgatc ttcttcactt ttgtagaaat
     5461 gattaatgaa gggcatcata gaataggcga aaccaccgcc gcccatcgtc accggattca
     5521 ccgaagcata catagtcatc gagcgccaaa acatcttccg aaccaatttt ttctcatctt
     5581 tagaaattcc atcagctaat ttcattgaaa gaaatcctcc tcttcttggt caagcgttgc
     5641 atttacagca gcatcgcttt gaactgcatg aacagatata ttttttagtt gtagatctct
     5701 ttgggctata acaattgcaa taatcgttcc aactgcggca attgcaatca atggtaactc
     5761 gagatacgcc ataacaacgg ccccaaagaa aaaccacaca gcaatatttt tactccaaag
     5821 catccgtaac agcatcgcca tcccaactgc cggcagcaga tttccagcga ctgttaatcc
     5881 gttcatgatg acctctggaa tattatccaa gacgcttttg acaacatctg ctccaaataa
     5941 aacggcaaag aatggaataa gagcataaag cgcatagtgg acaaaccaca taaagaagtg
     6001 cagattgatg attccttttt gatcaccaga ttttgcaaat ttgaaaaatt tagcagccat
     6061 cggtccacat acaaaagaaa aaatcatatt cattaactgt aatcctaata ctgctagtgg
     6121 aaagacaatg ggaataatcg catccacgtt tcctcccatt ttgattgcga acgctgtagc
     6181 cagcccagca gccaaagaag gctccgccgt actagagatt ccgatattga cgacacccat
     6241 aaagatggct tctagtgacg ccccgacaat caaccccgtc tgcaaatccc ccatcaacgc
     6301 tcctgctaac ggagcaacaa caatcggacg actcagcatg gacatcccga acatttcatg
     6361 tccagcaact ccaataaaaa cggccaaagc gactacaaat gcatcataca taatttttct
     6421 ccttcctttt tctaaaaatc actcaaaata gagatagctg aacgtttttc catcgccggt
     6481 gtaggctgaa aaacggtatc tggataaagt tcaactaatt cttttaagtc ttgaatttct
     6541 gacgtcgtta acatcacttc ctttgataag gttttcttat cttccgatgg cggtgtcatt
     6601 ttcccagcat tccccacgtt cattaattcg atttccgttg ggaaagcttt cgcaactttt
     6661 accgcatctt tgacattagg gactaaaacc atcaagcgca tgtccttcgc ccgcggatct
     6721 tttaacaaat gaatcgcgtc atcaactgtt ttaataatga ctttgacatt cgatggtgca
     6781 gccatcttca atgtcatctt ttgaatatcg ttggtagccg cctcatcgct tgctactact
     6841 aacccggtta aatttagact ctttgtccaa gtcatcgcta cttgtccatg aattaatcgt
     6901 tcatcaattc gcactgcttt aatcattttt cttcctccta aaaaaaatct tcctcttcat
     6961 ttactacatg tacctcatca agaatggtca tctttaattc ctgttggcat ttatttacca
     7021 gttcctgaat atcctccttt gttaattcct tggttgccaa tactatttcg agcagaattg
     7081 gcagattaaa gccagacaaa atagttacag caatctcttt ctccaaaaag gttttggtaa
     7141 tcttctgatt gacacttccg ccaaataaat cggtaaaaac aaaaatctga tcctgctcgt
     7201 tcacctggtt tacaaataca ttcaactcct gttcaaaatc acactcatcc acataagcat
     7261 taatcaccgt aatctcttga accttccccg tcagtaattg aatactgcta agaaagcctg
     7321 aagctaagtg accgtgagtc gctattagaa ttttcttcat atggttaatt cctttctttc
     7381 tgttcacttt tatataagca attcctgtgc caacttttaa aaacattggt ttaatcacgt
     7441 taattcacat taaaaaaagt gtctaaccta aaaaaaacag attagacact tttttagaca
     7501 ctttattttt gaatatacgg tcctaataat tcatagagca gcgaaagctc ataagcattg
     7561 actttaaaat tatatttcat ctccattgat tgaaaaatac ttctagaaac ataataaaat
     7621 tcatcttcat ctggtttcaa atcataattc ggttcttcag atttttctgt ccgagccaag
     7681 aataaacgtt ccatcatcaa tgccgtatgc atatagagat ttagttttac acggccattt
     7741 aaagtgaaat gatagtaatt ttcatattta gaaatgactg tttccacttc actcatcacg
     7801 atcgttgggt tcagaaagtt caaacggtcc gcaattcctt ccaacgaaaa gaattttact
     7861 agctcctgaa tcaagcggtt aaattctgcc ggcttcattt caggactcaa aatatgctcc
     7921 aaattggctg ctccatcacc gtctaagatg tcataaacat taatattagg aattgaaaag
     7981 gaacgtggaa gatccgaagt cgtgatgacc aattttgtct tgtcaaaata agtttcatca
     8041 ttttggtcga ttaattcacg tagttctctg tagtccttcg tgaaaacttc gatggattca
     8101 tccgataaat gatgacgaaa aacatctttc aatttgtccg aaatccccaa acccgacata
     8161 caggaaatga taatgttgct actttgagaa aagccttcaa agtattgaac attgattgaa
     8221 tacttgtctg ctgcatcctc cgcaatctgt ttaaatggcg cattcatcgt catcttcaag
     8281 ccaatatcaa gtgcaatgga agtcgtcaaa ttattcagaa gaagtaactc cccttttaaa
     8341 tcgttcttga tctgtgtgta aatttgattt aaagagccca tatctacgat cagcactaga
     8401 ctatctgtat tcacttgccg tttcacgaaa ttccttgtcg attcgacaat ctctgacaca
     8461 ttactctcta ctggcatatc caacgcttca aaaacataat tgccgcataa ttgattcact
     8521 actgcttgaa tgctgctagc cgtactatct ccgtgagcaa gcaacaggcc cttcagctcg
     8581 atggattcat ctacataatc agataaagtc acgacaataa gcagtcttag aaagttctcg
     8641 tccttttctg agagcggtgg taaatgctcc aagaactgat tacataagta agtcgtccga
     8701 ggtagatttg cagctaatgt acgatttatc ccggttaagt ctgtcttata gcttggggaa
     8761 atgacatagg atctgctaat aatttctgca acaggtccac tcgtcgtcaa tccgtacgtt
     8821 tttcctaaaa cggataccca gacctttcta aacatctgaa tcatcaattt tctactcatt
     8881 gataaaaaag aatccttatc aatgtgatgc accaaatgct gtacttcatt ctttatcgaa
     8941 gaaaaatttt cattgaaatt accttcattt atcagctgag taattttttc caaaatcgaa
     9001 tcataatcaa acggtttgat tcgatccgtt tttatctttt gatttttctt tatcaataaa
     9061 ctagcaatcg gcaagctttc tacttcggat tccagcatat ttaactgaat caagatctca
     9121 tttttcccaa tcgttaatgg ttcttcttgt gttcgactat acacatccgc acaactgatt
     9181 ttaatgatat tcttcagctt accaatattc ccactaaagg gatgagataa cattaaagac
     9241 aaggcttctc ttgaaacaat aatatctttt tgtaaaacat ccgcttcatt ttgaaaaaat
     9301 aattgaatca gctctaatcg ttctcgaatg gggcgatctt caaaagttgg caacataaca
     9361 ctaatttgaa tacgccgatc aaaggtgtct aataaataat tttcaccttt ttccgtcgtg
     9421 gcgaaaataa aacgaacctt agcagaatgc caatttacat tttcaccgac tggacgatag
     9481 tttccagtat caataaatag aaagagcttt tcttgatttt ctttcgatag gcgatggacc
     9541 tcatcgagaa aaagatagcc gttgttggcc gttgccaata aaccatcggt atccttttca
     9601 gcccccgtaa aggccccctt cttgtatcca aacagtgttg ccgaaagcag ttcgggattg
     9661 tccgcataat cggcacagtt taagatagca aatggtgcat ctttttcaat aatttgctca
     9721 tggatagcgt attcaaaaat ttttgtggct aaaaaacttt ttccaacacc cgtggctcca
     9781 tttattaata cactgagtcc gttaggcgga tatttgacag cggctataca tttttgaatg
     9841 acctctctca gacttccgtc atgaccaact acagaatgaa aggcttcgtt tatttgctcc
     9901 gtattatcta caaccgcata ttcatctaaa ggcttccaca aaacaggctt tcccggtaat
     9961 ttctcgacct ttttatcttc cactaatctc gtcaaataat ggctggcatt ctgacgagag
    10021 atacctagat tcttcgaaat tttctcggca gtgacagaag acatattccc tgttagtcgt
    10081 atcagctcat caaaaactcg atctttagcg ctctttttca ttttttcctc cagtttaatg
    10141 tgtctaaaag cataataaac tatcggctgc aatatgtcta ttctatattt gtattccaat
    10201 ttttactttg aaacacaa
//
LOCUS       DAGCWU010000103        10175 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.103, whole genome shotgun sequence.
ACCESSION   DAGCWU010000103 DAGCWU010000000
VERSION     DAGCWU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.103"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            165..359
                     /locus_tag="MBZ71_001965"
     CDS             165..359
                     /locus_tag="MBZ71_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429891.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            628..927
                     /locus_tag="MBZ71_001966"
     CDS             628..927
                     /locus_tag="MBZ71_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429892.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1024..1458)
                     /locus_tag="MBZ71_001967"
     CDS             complement(1024..1458)
                     /locus_tag="MBZ71_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4429893.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1690..2400)
                     /locus_tag="MBZ71_001968"
     CDS             complement(1690..2400)
                     /locus_tag="MBZ71_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBT4429894.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2390..3250)
                     /locus_tag="MBZ71_001969"
     CDS             complement(2390..3250)
                     /locus_tag="MBZ71_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBT4429895.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3255..3893)
                     /locus_tag="MBZ71_001970"
     CDS             complement(3255..3893)
                     /locus_tag="MBZ71_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBT4429896.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3908..4207)
                     /locus_tag="MBZ71_001971"
     CDS             complement(3908..4207)
                     /locus_tag="MBZ71_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4429897.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4250..5734)
                     /locus_tag="MBZ71_001972"
     CDS             complement(4250..5734)
                     /locus_tag="MBZ71_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBT4429898.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5754..6215)
                     /locus_tag="MBZ71_001973"
     CDS             complement(5754..6215)
                     /locus_tag="MBZ71_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4429899.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6233..7300)
                     /gene="ulaG"
                     /locus_tag="MBZ71_001974"
     CDS             complement(6233..7300)
                     /gene="ulaG"
                     /locus_tag="MBZ71_001974"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBT4429900.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7518..8288
                     /locus_tag="MBZ71_001975"
     CDS             7518..8288
                     /locus_tag="MBZ71_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4429901.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8561..9388)
                     /locus_tag="MBZ71_001976"
     CDS             complement(8561..9388)
                     /locus_tag="MBZ71_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4429902.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(9913..>10175)
                     /locus_tag="MBZ71_001977"
     CDS             complement(9913..>10175)
                     /locus_tag="MBZ71_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4429903.1"
                     /translation="PRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHSIRLKF
                     LKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT         3183 a         2061 c         1588 g         3343 t
ORIGIN      
        1 gaacaatagc cttttattat atatattatt caaattagtt atatcgagtg aaaaaaactt
       61 tcaaattgat tctttttatt tatcataaag atatagatac ataatcattt tcacttacat
      121 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
      181 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
      241 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
      301 acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc gaaaagtaaa
      361 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
      421 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
      481 atgattattt caatatgtat cctggaattg gagatctcgt agagctatat caagatgaat
      541 caaatatact tgtcattcat catgaaatat agagacttat aaaattgttt atttttataa
      601 tgtatcagta tagaaaggaa ataaaacatg gattatactg ttgaatttga cagtgaacta
      661 cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc ccacaaagtg
      721 acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa
      781 ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc atttgcttta
      841 aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat tgtatttgat
      901 aaagaggtga gtaaaaaaat aaaatagctg agcattggag gatagattga gtgatactaa
      961 gtctatcctc ttgtttttct aaagaactaa gctagaattt tttcttttgt acgttattat
     1021 atgttatttg tcattaccaa aaagcacctt ttttcttcta gtatctatga cataagaaat
     1081 accacccatt tcctttactt gaacaaatcc tactgattca taaaattttt ttgttttctc
     1141 agtatcatct gtcaataatt cgatttggac tggtccataa ctagtgatct tctccaaagc
     1201 ttcattcaaa agcactttgc ctattccttg gcgctgatac ctcggatgaa tgaggatatc
     1261 ttgaataaat gcgatcgtcg caaaatcagt gatccaacga acgattccga taatcttccc
     1321 ttccccatta cgctttatca gagattcaga ttgttcaaat gctctagcta accttgcagt
     1381 gtcttttgtg taattcgtcc aaccaacgct cttatataat tcagaaatct catcgatcat
     1441 aagggaatca atattcattt ttttcttcct ttcttataca attctccctc taataataca
     1501 gttctttgac tatttaatct atcacatata actctcctgt aaagattctc tcgattcctt
     1561 aaaattcctc tttcctttta gaatgcatac tttttgataa ataaaaaggt tgtgataaat
     1621 tttcttcaca acctctgttt ctctctatca agtgttatct tttaattttc tacccttacc
     1681 ctagcatctc tattgcccgt agtatgcata ctttccatgt ttgcgaaaat aatgtttatc
     1741 tagtaaatat tgcgggattt cacatatatt ggggttgatc agctcgtttt ctttggccat
     1801 cagacaaatt tcatctaaga tcaaactgtt ttctactgct ttcataggtg aatcccccca
     1861 tgtgaacggt ccatgtccat agacaagtac tccagggact tcgtttggat ctaattttct
     1921 ttccttaaac gtctcgacaa tcacattccc tgtatgaact tcgtaagctt ctcttacttc
     1981 ttctttcgtt aattggcgtg tacaaggaac cttaccataa aaagcatcag catgagtcgt
     2041 gccataagct ggcaaatcgc gtcctgcttg cgcccacatg acggagtgcg tggaatgagt
     2101 atgagtaata gcagttatat cttcaaaagt ttgatacaaa acaacatgtg tggggagatc
     2161 tgaagatggt tttaagctat cttcttctat tatatttccc gacaaatctg ttaccaccat
     2221 ttgatctgct tgcatgcatt catatctgac accgcttggc ttaatgacga tgactcctag
     2281 ctcacggtcc acttcactca cattgcccca agtcaatttg actaatccgt atcgcggcaa
     2341 tgccagattt gcttcaagga cacgttgctt cattgctgca attttttctt cacaagacat
     2401 catagccagc ctccttcaat ttagaactta agtagtcttt tgcctgctgg atttctcttc
     2461 gaaagtcttg gcttttttca ctccacattt caatcagaaa tgttccatca taatctagac
     2521 gtttcaaatg tcttaataat ccaagaaaat caacacaacc gctaccaaaa ggaacatcac
     2581 gaaactggcc tgaatattct tttgttacag ggtaagtatc ttttaagtgg atagcagtga
     2641 tataatcgat ccctttctca agttctcgag ccggattatt ttccggccat gcggacaaat
     2701 tgcctaggtc tggatatact tgtagaaaag gtgaatgaat ctgttctttg atttctaaaa
     2761 attttgaaat ggagttcata aatggatcat ccatgatttc cacggaaaga atgatgccat
     2821 atttagaagc ttcctttacg ctttctttta ttccttcgat aaagtcttct ctggaattca
     2881 ttgatttctc ttcataataa acatcgtatc cagctatttg gattactttg acgcttagat
     2941 tatgcgccaa atggattgct tttttcatta gttgtttcgc tgcttttttc ttttctggat
     3001 cagcagaacc aaacggaaat cgccgatgcg cacttaaaca aatcgaatgg atacgtacac
     3061 cggagctgaa catctcttct ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg
     3121 ctaaccgctc atccgtctca tcaatcgaca tctcgataaa atcgaagtcc atttctttta
     3181 caagggaaaa acgttctttc catgaaatat ctttgggtaa cgctttttca taaatcccaa
     3241 tttgagccat ttccttaccc ccagatacga atgatctcat ctttgaagtt ttgtgcggcc
     3301 ttcatcggat catttgctgc tgtaatacca cgaccagtga taaaggtata cacatcgatc
     3361 ccttcaaata agtgtaatgt gtgtggattt aatccaccag ttacagaaac attaaagcct
     3421 agttcaatca gttttttgat cttcgataaa tctttttcac cccaagtttc accagataac
     3481 agcgcatcac ggctttgatg gtagattacc tgccggatac cgatgttatg ccaatccatg
     3541 gcttgttcaa aagtccaatt gccgtagagt tctacttgca attcacctat ttctttttgt
     3601 gctgcctcca tagttggaat cgttgcacaa caaatgactg tcatccagtc tgctcctgct
     3661 tttgcaacat tactcgccac tgttcctcca gcgtctgcac atttcgtatc agcgactagt
     3721 tttttatctg ggaacatact gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa
     3781 agaattgtgc ccacttcaat aatatctact acatcaccaa ctttcataac atctcctaat
     3841 gcatgttcta agtcattgtg atctaacgca atttgcaaat tcggcctgct catcttagtt
     3901 ccttccttta cttgtttttt tggtaccact ttgtttcagc aaatttttca ctgatttctt
     3961 tgactgacat cacatttttg ataccgataa ctgtaactcc ttttttctct gcctgagtaa
     4021 acatattcaa aaaattgact ggcgtaaaaa ctacatcgta ttggccagct gaactttttc
     4081 cctctgaaat ggcacaatga tggattttgg taattggtac gtttagttct ttcattgctt
     4141 tttccacact tctcatcatc atcaagcttg ttcctgatcc atttgcacac gatactaaaa
     4201 ttctcatctt aatccctcca ttatgagtgt tgtctaatca gattttcttt tatactctct
     4261 gcttttctgc atatgtgtca tagtcttcta caatcaaaaa ataaccatca ggatttttcc
     4321 gatattgtaa ctgcggaata gccaataaac caatgacaac tacggcaatt ccagcataac
     4381 ctaggacttt catgattact gtaaacaatg gccaagctgt tgcccagtcg aacatcccga
     4441 tataaccacc gtatgttgac atcccaatcc atgtagcgaa taatgctgaa ccgcctactt
     4501 gtatgattcc tgaaagaaac gggaacaggc aagcggcttt gaatccacct cggttattgg
     4561 cataaactgc tataacggca ttatcaaaga acaacggaat gaaaccggct atgacgatcg
     4621 ttggtgattt caataaaatc agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca
     4681 agaacccaat cgttaccgca tttggcgaac caaagccaaa agtagcagct acgtcaattc
     4741 ctggaacagc tcctggtaaa atcgtgtttg agattccttg gaaagattga gttagttcat
     4801 cgacgaatgt ccgaacacct aattgcaaga tcgccagata taccgcaaaa tttaaagaag
     4861 tcgtcataat gtagaataag aaactctgcc cttcttgcat gaattgcgct tgaatcaaat
     4921 aatctttgcc tagtacaatc agaatgatcc caaagaaaaa tagcatgagt aacgaagtag
     4981 caaccatatt ctcattgaaa atcgacaaga atcctggtaa tttgatatct tcaatttttt
     5041 tgtcttgctt gattgatcta tttttctttt catcccgctt tttcatccac tcagctaatt
     5101 ttgcaaatat aaagattccg aacatttgct gatgtgccac tgcaaatccg gcaccttctg
     5161 tcaattcctg cgtgattcct actgttaagt tagaagctac tgcccaataa caacctaaaa
     5221 tcagtcccat aaaaatcaat acttgtatcc tacctagatc aggaaagcaa aacagtaaaa
     5281 tccaaaaagc ggtagctgct tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca
     5341 gtttcgtata tttctgaaaa cggactaata agatatttaa aacaaatgca atcagtaata
     5401 aaatcatcac atctccaaag gtacggccaa atgtctccat taatccagca tccacagcat
     5461 tttgtccaaa ataaggatct gtaaccatgg catccaaatt gaaccgttcc tttagtccaa
     5521 ccaagattgg ccgaaaatta ttgaccaaac caccagagcc aacagtcaaa ataaagtaac
     5581 cgaccgttgc ctttaagaat ccagcgatgc attcatgaag cgggcgtttc agcaaaatat
     5641 agcctaataa cacgataaaa ccaatcaggt atgctggctg cgtcaagata tttgttgcaa
     5701 aatacgtcca aatgcttaac aaaaattccc ccatcttttt tctcctctat caattaatta
     5761 tattctgcca tcacaacttg gtaatcatcg atggagttcg tagagatgag acgctcaacc
     5821 aatccatctg tcattaataa ttcggataat ttttggatat tttctagatg ttcttctgga
     5881 tcttttgctg cgagtgtaaa aaacaaacta gccgttttct cctcattctc cccttcactt
     5941 tcaaaacgga ttggctgttt caacttggta aatgaaatag ctgttccaaa aacaccatca
     6001 cttttttctg aagaatgcgg cattgctact tgaggtgcga tcacaatata aggaccgtat
     6061 ttttctacac aatggatgat ctcctccaca tacgtttcat caataatttc cttatcgatc
     6121 aatacaagac agctttcggc gatagcttca cgccaatccg tgatttcttt atctgggtaa
     6181 tgaatcaatt gatggtcaaa aaagtatttc agcaagaaac tccccttctt tcttataaaa
     6241 acgacggaaa cggtgtatca ttttcaatcg agaagacatc ttcaaatcct cgataaaaat
     6301 taaactcaag atcatctttg tttgtcggat agatgaattt cccgcctact tgccagatat
     6361 aaggtttaaa tccatacttc aggcgatcct ttttgaattt ccaaatttca gtgatctctt
     6421 ttggatcagc cataaaatta gaccaaatat cgtagtggac aggaatcact acttttgcat
     6481 tcaatgactc cgccatgcgc agcatatcaa cagaagtaac tttgtctgtt atccctcgtg
     6541 ggttttcacc ataggcccct aaacagacat cgattttgtg ttcattacca tgtttggcaa
     6601 ataaattgga gtaatgtgaa tctcctgcat gataaagatt ccctcctgat gtttcgaata
     6661 aatagtttac tgcaatttga tccatctcct gaggcatttt tccttttaat acctcttctg
     6721 gatcttcaca agtgaccaac gctgtccgat caaacgcttc taaagctact acattgatgt
     6781 ctttgatttt gactgcatca cctggcttta ctgtaatcgt tttttcttca ggaattcccc
     6841 atttttgcca cgtgcgaacc acttcttttg gcccaatgaa tgttgcttca ggacaatttt
     6901 gatgaactgc tgcggctgta tggatatcta gatgatcgga atggatatgt gtaacgatta
     6961 aagcgtctac attttttatc gcaaaaggat cgattacgaa aggctgagta cgtaaattag
     7021 gctgcatatt ttgaacgcca ctcatacgca tcatctgatg gccttttttc atcatgccat
     7081 tcccatgact acgttttccc gtcccacacc atagatcaca taaaatattc gttccttcat
     7141 gacttttcag ccaaattccc gtacagccca gccaccacat agcaaaagtt ccaggatcca
     7201 ctacttctgt ttcgatttct tcattcaagt atgtgcccca ctcaggaaat gtttctacta
     7261 accacttttc ttttgttact tcattgacag ttttactcat ttgcttgctc cccccatatc
     7321 gttgttccaa cttacaagta aatattaatg tgtatcagca tccatgaaaa cccttttata
     7381 tgaccgtttg ttttttatat gattttcttt cagtaatcaa tgattgtttc agtataataa
     7441 agacagatat gaatgtttaa atgaacaact agtcaaataa atccatggat caagatgtcc
     7501 ctcaggaggc aaaagtcatg aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa
     7561 ttttggaact gcttcagcag actgaacaac tatcaacttt cgaattagct gaatcccttg
     7621 atgtatctat tagtacgata cgaagagatt taaatttatt agaagaaaaa aacgagatca
     7681 tacgcaatta cggttattgt tcttataatt acaaaaacca gactgatttt gatcaatctg
     7741 gtcctgaaag gattaaacaa gcgattgcca gagaagctag taaatacatt aacgattatg
     7801 atacgctgtt tatcaattct agctcgactg cgttgaaaac actggatttt cttcaatcta
     7861 accatttgac agtcattaca aacaacgtga aaatcaatta ctctccgcac aaagataatt
     7921 gtagttatat tttaactggt ggagagatgc gctttccaaa agaagcacta gtgggcgaca
     7981 tcgcattaaa tacgatttct tcagtcaatg cagacatttg tatcattggc tgtagcggag
     8041 tcgatgtagt acatggggtc acaacaaaga tattaaatga atcaacgatc aacgaaatga
     8101 tggtcaacaa gacaatcaaa tgtaagattt tagtcgctga tcatcgaaaa atcggactga
     8161 catcaaagtt caagattgct gatatttcag tctttgatta tttgatcact gataaatttt
     8221 gttctgaaaa agtattagag gagatcagaa atacggggat aaaaaccatt caagtagatt
     8281 cattttaaga accaggacgt aaatatgtaa ataggattat caaaaaaatg aataacaaat
     8341 ttatcgataa atgaaaaaca cgctacgctc aaattgataa aggaatccca agatgtatcc
     8401 atccaatcga taccatcata atctattgct tcttttataa ccgttatatg ttgcctaaat
     8461 aggaatcaaa caatatcgaa aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa
     8521 taaacggtat tcaaatcaga acactgattt cacaaccact ttatttcagg tcgttttcaa
     8581 tcacctttac cgcgtaatca tttccgttga aacgcgctat tttttttgcg tccatatatc
     8641 tttctaaata atcatcttct cctaattgct tatggcaaga agcaatttca taaagcaagt
     8701 attccaatgc ataagtcgat ttatgtttct tattatactc aatacccttc tcacataatt
     8761 ggattgcttt atcataatcc tcgattttac ggtaataggt ggcgatatca taatatacaa
     8821 tgcagaccat cagattatca aaatcagtct cactcaatat tttaagtgta cgctccacat
     8881 aaactttcgc aaaatctaat tctccttttg acaagtagat cattgccacg tttgcagctg
     8941 cttgtaagtg atggatatct gtttcggtcg tttgttccaa cacttgatta aaataaaata
     9001 atgctgcatc tggttttttc gcataaagat gatcaagcag tccttgataa aataaaattt
     9061 gatttaaaag atcagaatta cttacttttt cctgatcgat cgtttgtaaa attttataag
     9121 attcttggta ttttgataca gaattcaatg attgaatctc atttattttt ttcctgattt
     9181 tgtcttcttc tgtttctact aatatatcgg agatctcgac attcagcttt ttgcaaagcg
     9241 cacttaagat attgatgcct ggcgacatat tcttgttttc gatattgctg acagttgctt
     9301 gttcacaaat gccttctgct aattgtactt gtgttaaccc ctttttcttt cggtattctt
     9361 ttagtaatgc tccatctact ttaatcaaaa caatttcctt ccctttaaaa aattatttct
     9421 cctttatatc atacacaaaa aaaagttcta aacaaccaaa aatgtttaga acagaataaa
     9481 aattttaatt ttaaaataat attcaaatta aacaactggc gtatctgctc cttgatttct
     9541 ttttgtgtta tctgaataat tatcgtttac agttataaca catagtgaaa attgtcatgg
     9601 cgagaaagtc taagaatatt tttaaacata actatttctt tcttacgtgt gtataatgac
     9661 gacagcacac ctatcaatta atataattat tttatttttt atgttctcac ctgaataatt
     9721 catagcttta attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac
     9781 tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt
     9841 ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt
     9901 tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa
     9961 ttcattctga tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac
    10021 taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg
    10081 gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag
    10141 catcattcgg acatgattct ccaaaaagcg aggac
//
LOCUS       DAGCWU010000104        10143 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.104, whole genome shotgun sequence.
ACCESSION   DAGCWU010000104 DAGCWU010000000
VERSION     DAGCWU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.104"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            122..622
                     /locus_tag="MBZ71_001978"
     CDS             122..622
                     /locus_tag="MBZ71_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4429904.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(812..1156)
                     /locus_tag="MBZ71_001979"
     CDS             complement(812..1156)
                     /locus_tag="MBZ71_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBT4429905.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1310..2230)
                     /locus_tag="MBZ71_001980"
     CDS             complement(1310..2230)
                     /locus_tag="MBZ71_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4429906.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2417..2908
                     /locus_tag="MBZ71_001981"
     CDS             2417..2908
                     /locus_tag="MBZ71_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBT4429907.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(2932..5307)
                     /locus_tag="MBZ71_001982"
     CDS             complement(2932..5307)
                     /locus_tag="MBZ71_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBT4429908.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            5633..6259
                     /locus_tag="MBZ71_001983"
     CDS             5633..6259
                     /locus_tag="MBZ71_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBT4429909.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6282..6986
                     /locus_tag="MBZ71_001984"
     CDS             6282..6986
                     /locus_tag="MBZ71_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4429910.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7178..8011
                     /locus_tag="MBZ71_001985"
     CDS             7178..8011
                     /locus_tag="MBZ71_001985"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBT4429911.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            8208..10136
                     /locus_tag="MBZ71_001986"
     CDS             8208..10136
                     /locus_tag="MBZ71_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBT4429912.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFSESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         3146 a         1914 c         1852 g         3231 t
ORIGIN      
        1 tgtattgttc gatgattctt aatacgatat gacacaataa ataaaaatag gcatggctat
       61 ttacaacaat agtgctatag tagaaaacaa atgaataagg ttacttaaag aggtgaatac
      121 gatgaaactg atcaaactat gggaagcaga tttaaagaaa gcttatcagc tacaaactag
      181 ctttggacca aatgagagtg gatttatcaa caatgcttat ggctatacat ttgctgaatt
      241 tttagattat gtaaaaaaat gcgaaaaaac gtctttagga atcgatcttc ctgagagtta
      301 cgttcccgca accgtcttta ttttagaaag tgatgaacat gagtatgtag gaatttttaa
      361 tttgcgccat aaactgaacg aaacattaag aaacggcgct ggccatattg gttacggcat
      421 ctcccctaat tatcgtaaaa aaggatatgc cacacaagga ttagcattag tactacaaga
      481 ggcaaaaaaa atcggcattg atgaggcact tttgtctgta aataaaaaaa atcttggcag
      541 tcttaaagca caacaaaaaa atggggctgc gatttatcgt gaagatgaga aagaatacta
      601 tacgaaaatc aaactgatgt aaaaaagaag gattccaaaa ggaaagggat ttgaatgtcc
      661 tttatcaata gaacacattt atttaattaa cttataatat agcagacaaa attttataaa
      721 caaagaaacc ttttatctct tttcttctag aaaggataaa aggctttttt gtttttccta
      781 tttgcttctc atagctagtc agccaagatt tctatcgtat ccccaacgca atccgtgcat
      841 aacggctcat cttatctgtc gtccatgctg gataccagac tagtttgact tctgctttcg
      901 ttacttctgg cacttctttt aatgcgtcat ggatagattc tgttaaaata tctgctaatg
      961 gacagcccat agttgttaga gtcatcttaa tgactgtttc tccattttca ggattgaatt
     1021 ctatatcata aatcaagcct aaattgacga tgtcgattcc taattcagga tcaatcacca
     1081 tttccaaggc agcaagaata cgttctttga ttagttctat ttcttgtcca gaccgttctt
     1141 ggtttgcttc gctcatcgta cgacactcct taacacgatt tttatttatc atactctttt
     1201 tttagctgct tgtaaatgag aatttagaag taagattctc gtttaaattt ataaaaatgt
     1261 tcttattaga tgtaatagat taaatttatt ctaaaataaa cctgattcct catttttttt
     1321 gtgaacgatt ttttatttct ggcaatacgc cagttgttcg atacattttt tgttcgtttt
     1381 catttgcaaa ccggccaaat actgaatgag ttggtaaaat attgataaat gcatcgtcgt
     1441 cgatttcatg aacgatctga tcaagatcat acatttcgta gcgggtgatg accatcatga
     1501 tcatgttccc ttgttttttt gaatagccac cgacagacgg cagtaatgtc attccacgta
     1561 ccatccgttt tgctagttct tctgttacct cttctggctt gacggtaacg atcattgctg
     1621 ttactttttg atgactcgtg tggatggcat caattacttg agtcattgca taaatggaaa
     1681 taatcgtata caaggcactt tcccagttaa agaagaagcc tgcgatgact acaatgatac
     1741 catttagaat cagcatgtag tttccgactg tttttccagt tgtttgtgaa agtacaagag
     1801 agatgatatc catcccacca gttgtaaagc ccatttttaa agataatcct acgccaagac
     1861 ctaataaaac accgcctact agggcattca tcaaaatatt atcagtgatc tgtccgactg
     1921 gaagcagcat cgtcatcacc gatacacata caacattcac aaaactaagg atcgttgctc
     1981 gttttcctac tttgagaaac cctaatacaa acacagggat attcaaaaga aaaataaatg
     2041 ttcctgtgtc tatcttgata tgaaaccatt catataaaag cgaggaaatg atttgagcga
     2101 tcccattcat ccctgcagaa aagacattgg ctggaatcaa aaaataattt aaagcgaccg
     2161 cagcggtcaa tccggttatc aaaataacca ctattttctt taatctttca tttttatgat
     2221 acgcagtcat aattttactc acaagcacct ctcccctact ttggtattcc aaatgtaaga
     2281 ataacagttt atctattaaa ataaaatact tcgaactcaa atgagaagaa ttttagcaaa
     2341 caggtaagaa tctgccctaa tcctatgata agatagatgt atggtaaata tttatatgta
     2401 tatcaggagg gttcctatgg caaaagatgc tagcttcgac attgtatcag aagtcaatac
     2461 agaagaagta aaaaatgcga ttcagcaaac aacaaaagaa ttgtccaatc gctttgactt
     2521 caaggattct acagtagaaa tcaaattaga gaacagcaat ctagttgtat tgagcgatga
     2581 tgatttcaaa atcgaacaga tcaaagatgt attatttggt aagctgatca aaagaaatgt
     2641 gccaatcaaa aatatccatt tttccgaatc agaacacgct ctaggtggca aagctcgaca
     2701 aactgccgat ttggtcaacg gaatcgatcg tgaaaacgcg aagaaaatca ctacagctat
     2761 caaaaatgaa aaactgaaag taaaagcaca aatccaagaa gatcagatta gagtgacagg
     2821 taagaaccgt gatgatctac aagctgtgat tgctttatta cgtaaacttg acttaccaat
     2881 cgagttgcaa ttcactaact atcgttaatc aaaaaacgag gctgacgtct gtcattcctc
     2941 gtttttttga tttactgctt cttttgataa ccaaaaacgc tgtattcctt cttccaattc
     3001 cccaacactt cgtacttctc ttgctgtttg ccaatgttgt gggaataaac gtcgataaga
     3061 aaaactagaa aatcgttgat ctgccaatac aacgattcct tgatccgcca tgccacgaat
     3121 cactcgacca ccagcttgca agactttgtt cattccagga agctgataag cgtaagcaaa
     3181 tccaaggtgt tccttctcat cataatatga tttgatcagt tcctgctcat gattcatctg
     3241 cggcaaacct acacctacga tcatactgcc gatcaatcgg tcccctctca aatcgattcc
     3301 ttctgaaaaa atcccgccaa gtacgcaaaa tccaatcaat gtttcagcgg gatttgtttg
     3361 aaattttgtt aaaaatcgtt cgcgttcttc ctcattcatg tccgtttctt ggattagcac
     3421 attgctgtcg ggatattgct gtctgaatgc ttccaccgca agatctaaat attgataaga
     3481 aggaaagaaa acgaaataat tcccgacctt tccatcagca aaagcatgaa tcgtctctac
     3541 taattttgcc agactgtgtt ccctttttcg ataagtcgtc tctaggtaat ttgcgatgaa
     3601 aacttgctgg tttccttctg ggaaaggact cggcagtttg taagccagac ttttttgtcc
     3661 ccccagtgtt tcctgataat aagagagtgg tgaaaaacta gcagaaaaaa gaatactgct
     3721 ttttccttta tccaacgact gctcaagaaa caagcttgga tcaatacaaa actcttttac
     3781 tatcagatcg cgatacgttt tttcgactgt tgtttcgtag tgatcatcat aaaattcact
     3841 gaccttcaaa aaatgaagca aatcaaaata ataaggaagc aattgttctt gtgatgggtg
     3901 ctcaggaaat tctgccagcc attctttaat tttttcgctt aattgataac ctgcctttac
     3961 taaagattca gccggtgctt tttgatgatg ataatcccaa tgatcttctt ttgctatttc
     4021 tcggatcgaa tcaaattctt ttaatagttt gttgaaacgg cgatgaagtt ttttgaattc
     4081 ctttggtata gcttccttac ttcgtttcgt tttttcataa gatagttctg ccgaatacat
     4141 ctctcgggaa cgattcacca aattatgtgc ttcgtcaatc aaaaacagat agtcctcgtt
     4201 tttttcatct tcaaaaaaac gtcttaaata aacagttgga tcaaataaat aattgtaatc
     4261 cccaatgatt acgtcacacc agatactgac atctaatgat agttcgaaag gacaaagtgt
     4321 atgctttctt gcataagttt cgatgacttc acgagtgatc tgattctcat gattcaaaag
     4381 atcccataaa ccttcattaa tccgattgta ataaccattg gcatatgggc actgatctgg
     4441 attacaggtc gtctcatcta aaaagcagat cttgtctttc gccgtcaaag tgacactttt
     4501 tacttcagct cctgtatctt ttaatgctgt catagcatct tcagctactt gtctagttat
     4561 tgttttagcc gttagataaa agatcttttc tccctcttct tcccctacag ctttcaaagc
     4621 ggggaataac gtagagattg tttttcctgt tccggtaggt gcttctacaa acaatttctg
     4681 tttcgtacgg atcgttttat aagcagctac cgctagttca cgctgacctt tcctatactc
     4741 atcatggggg aatttcaaag ccatcaaaga ggcattgcgt acagtgcgcc agttttcttg
     4801 aaaaacaagc cattctttat aatcttcagt cagcttttta aaaaaagttt ctaactgctc
     4861 tcttgtctga tgttcaacct ttctcgtgat cacttcttct gccgtctgga agtaagtcaa
     4921 ttgcagattt atttcttgta aatcatgttc ttgactatag atataggcat acacccttgc
     4981 ttgatgaaag aaaagttcca cttgttctgg ttctaaatct tcaaatcgtg gttcggaggt
     5041 tttgatttca tctatataat aaataccgtc tcttttgaaa ataccgtctg cacggccttc
     5101 aacgatcagg taatattcat ctaattgaac tgttgttttt aaaaagactt ctttttcata
     5161 gttttctccc gcatcttttt ggagcttgcg gtggattctc gctccttcta atgcagtatg
     5221 gttacttttt ttacgattgt cgatacttcc acgtcgcaaa ataaattcta ctaagtgccg
     5281 cacagatatt tttgactgtt tgatcatttc caccacctct ttctctctaa gattacctga
     5341 aaaaacctag tttagcaaaa agagctctct ttccaaacat aaaaaaacac cctgctgatt
     5401 aggcagggat ataggatata gaatttggat ttttggatga atctaatgta acaaggacat
     5461 attaaaatgc tatgaaaact tactttcctt ctctttagaa tatccaaata cttttcatat
     5521 agtctgataa ttcagataag taaagagaaa taagtacctg tttttgtttc ctagcaatga
     5581 atgcgttata ctaatggatg aaagaagttt tggctatcaa ggaggaatgc acatgcatat
     5641 cgaaagatta aaaacaggaa tgattgaaga aaactgttat ttggtctaca acgaagaagc
     5701 acttttgatc attgaccctg gagaagatgc ggaacgaatc aaacaagaaa tcaaaaaaac
     5761 aaaccaacag ccggtagcga ttttgcttac ccatactcac tacgatcata tcggtgcagt
     5821 ggaaccttta cgcgcgtatt atcaaatccc agtttatgtc agcccactag agcaaaaatg
     5881 gctaggtgat ccgattttga atctttctgg actcggtaga catgatgata ttgctgatgt
     5941 tattgtctcg cctgcagaat atgaatttga aatgaaaact tatactttag gaaacatgtc
     6001 ttttcgcgtc gtccctaccc cagggcattc tataggaagc gtcagcttta tttttgatga
     6061 ttttgtcgtt tctggcgacg ccctgtttag aggaagtatc ggtagaaccg atctgtatac
     6121 agggaattta gaacaattac ttcatagtat tcggacccag ctgtttgtat tacctgatga
     6181 atttgcagtc tatccaggac atggcgatgc aacgacgatt gaacaggaaa aaaggacaaa
     6241 tccttttttc aatcgttaaa acgacttagg aggagaataa aatgacagaa actacacttt
     6301 atatcgtacg acacggaaaa acgatgttca atacaattga acgagtacaa ggctggtgtg
     6361 atacacccct cacaaagcaa ggacaagaag gcattcatta tttaggtcgc gggttaaaaa
     6421 atgtcgactt ttcatttgct tattctagtg acagtggtcg tgcaatcgaa actgcccgta
     6481 ttattctagg agaacattct aagggaaaag aaattcctta ttttattgaa aaaaggatta
     6541 gagaatggtg tttcgggtct cttgaaggtg gatatgacat ggaaatgtgg ggtgtgatcc
     6601 ctcgtgtatt gaattttaaa acatatgatg aaatgtttac cacagaagtt tcttttgaac
     6661 aaattgctaa tgctgtatac aacttagata ccgctgggtg ggcggaaact tatgaaacat
     6721 tgcgagaccg tgtatggaca ggatttgaag atatcgccca tcactgcgaa aaaaatggtg
     6781 gtggaaatgt tttagtcgta tctcatggtt tgacgatcgc ctttttgctt aatctgatcg
     6841 atcctaaaca gcctgttcgt gctgggcttt taaatggcag cgtaacaaag gtcacttatg
     6901 caaacggtcg attttctatc cacggcatca atgatacgtc ttatatcgat aacgggaaaa
     6961 cacatagtcg cgactcgttt ctataatggg gatattttaa tttttaccag agaatcataa
     7021 aaggcaaagt ctgaacgagg gctttgtctt cttttttctc ttcgtgtact tatctgcttt
     7081 atttctcaag tttttcttaa tattaaaaaa cagattgggt ttctcaaaaa ataacggtat
     7141 actataagat gtatcttaat tgggaaggaa ggaatttatg ttttttgcaa atattatcaa
     7201 agcagtgatt ctcggtatta ttgaaggaat aacggaatgg ctgcccatca gtagtactgg
     7261 tcatttgatc ctagcagatg aattcatcaa actggatgcc agttcacaat ttatgtcgat
     7321 gttcaatgtc gttatccaat taggggcgat cttggctgtc gtcgttcttt attttcacaa
     7381 gttaaaccca ttttctccaa gtaaatcaac ggtagaaaaa aaagatacgt ggacactttg
     7441 gtttaaagtg atcgttgctt gcataccagc catcatcatt ggtttgcctt tggatgattg
     7501 gttagaagca aatttccaca agttcttacc agttgccatt gttttgatcg tttacggtat
     7561 tgcctttatt attgtcgaaa aacgcaacaa aaacaaaaca cctcgttgga cttctctgaa
     7621 tgactttacg taccaagcag ctcttcttgt tgggttattc caagtcttgg cacttgttcc
     7681 cggaacatcc cgttctggcg caacgatcct aggatctatc ttgattggtg cttcgcgttt
     7741 tgtcgctaca gagttttcat ttttcttagg gattcctgtc atgtttggcg caagcggctt
     7801 gaagatcgtc aaatatttag ctgaagggaa ttcattcaaa atggaagaaa ctgttgttct
     7861 tcttgtagga acattcgtat cttttgtggt ttccgtctta gctatcaaat tcttactaag
     7921 ctatttgaag aaaaacgact ttaccgcttt tggctggtat cgtattatcc ttggtatcat
     7981 tttgattggt tattggttta ttgcaatgta gaaaaaacca gaacacttct cttatttgag
     8041 aaatgttctg gttttttatt ttctttaata ataatcatta taaataataa aaacaacatc
     8101 aagcttaatc accaaatgat aaccattatc gttctcattt agctgttatt attatcaatt
     8161 acatttttat tatgctatcc ttgttttgac aaggaggtct aataacgatg actaatcaga
     8221 aaaaagcttt tgcagcaaca atcggctgcc ttttatttat gatcttcggt tttatcttcg
     8281 accgattctc tcttaccatc accccacttt tttatatcag tgccattatc atcggtggat
     8341 ataaacaaac atcggaaggc ttgaaagaat tgtggaatga tcggacactg aatgtggatt
     8401 tattgatggc tttagctgct attggtgcct gtttgatcgg taattacttt gaaggagcaa
     8461 tgttgacatt catcttttgt ttaagcggtg cattagagga atacacaact aataaaagtc
     8521 aaaaagaaat tactgctttg atgaatcttc aaccacaaaa agcccaatta ttaaaagaaa
     8581 atggacaaat gcaagaagta gatgtttctc aactaaaggt ggataacttg gtatttgtcg
     8641 ctaaaggagc agctgttccg atagatggct tttcagaaag cacacaggca acgatcgatg
     8701 agtctgcatt aagcggtgaa tctgtgcctg tagaaaaata tttaggcgat gagatgtatg
     8761 caggaacgct gaatcaagga aatcccatca cgattcgagt aacaaaaacg agttccgaaa
     8821 ctgtctttgc taagattatc caattagtag aagaagcgca gaatacgcca actcaaacag
     8881 cttcatttat cgaacgaatc gaaaataatt atgtaaaatt gattttgctt gctgttcctc
     8941 taatgatcct tctcccccac tttttcctag gttggagttg ggatgaaagc ttttatcgag
     9001 gaatggtatt attagttgtg gcttctcctt gtgcgcttgt cgcatctgct acaccagctt
     9061 ctctagctgc tctttcaaat tctgcaaaaa atgggatttt aatcaaaggc ggtatccatt
     9121 tagaaaagct ttctgaatta aaagcgattg cctttgataa aacaggaacg ctcactaaag
     9181 gaaaaccagt tgtaactgac agctttttct ttgatgaaaa ggatttggca caacaacttc
     9241 ttgttgctat ggaacaaaaa acgacgcatc cacttgctca tgcgattttg caatatttcg
     9301 attgcacaat acctgaagaa atccaatact taccaataga agaaattact ggttttggat
     9361 tacaaactac ctatttaggt gcgcaatgga aagttggcaa gcacgcatat gatcctgaga
     9421 cgatgataat cagcaaagag attgcggaaa tgatcgaacg tttggaaaat caaggcaaaa
     9481 ccgtgattta tctttctaaa gatcagcagt taattgctgt tttaggtctg ctagatattc
     9541 caaaagcaaa tacccaacaa gtaatttcct actttaaatc ccaaaacatc catacaagta
     9601 tgataaccgg cgaccattct ggcacagcaa aggccattgc tgaacaagta gggattgaac
     9661 attactatgc aagctgtact ccggaagaaa aaacacaact agtcaaaaaa gagaatgaac
     9721 tttaccaagt aaatgcgatg gttggtgatg gagtcaatga tgcgcctgct ttagcagccg
     9781 cttctttagg tgtcgctatg gggcaaggga cagatgccgc tatggatatc gcagacatcg
     9841 tcttaatgaa aagcgactta aataaatttg tctacagtca tcggctatct ttaaaaatgc
     9901 ggcggatcat caaacaaaat attatttttt cgattcttgt tattctcttg ttgattgctt
     9961 ctaacttcct gcaattttta aatctacctc ttggagttgt aggacacgaa ggaagcacaa
    10021 tattggttat tctgaatggc ttgcgtttac taagaccttt acctgatctt tctgattctt
    10081 caaaaaaatg taagtcttgt ccactataca aagcgcggct gtcttctgtc aaataagcaa
    10141 agt
//
LOCUS       DAGCWU010000105        10132 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.105, whole genome shotgun sequence.
ACCESSION   DAGCWU010000105 DAGCWU010000000
VERSION     DAGCWU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.105"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            116..622
                     /locus_tag="MBZ71_001987"
     CDS             116..622
                     /locus_tag="MBZ71_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBT4429913.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            639..1538
                     /locus_tag="MBZ71_001988"
     CDS             639..1538
                     /locus_tag="MBZ71_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBT4429914.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1625..2509
                     /locus_tag="MBZ71_001989"
     CDS             1625..2509
                     /locus_tag="MBZ71_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBT4429915.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2701..2772)
                     /locus_tag="MBZ71_001990"
     tRNA            complement(2701..2772)
                     /locus_tag="MBZ71_001990"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2737..2739),aa:Arg,seq:ccg)
     gene            2832..4166
                     /gene="rpoN"
                     /locus_tag="MBZ71_001991"
     CDS             2832..4166
                     /gene="rpoN"
                     /locus_tag="MBZ71_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBT4429916.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4315..5406
                     /locus_tag="MBZ71_001992"
     CDS             4315..5406
                     /locus_tag="MBZ71_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBT4429917.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5447..6448
                     /gene="gap"
                     /locus_tag="MBZ71_001993"
     CDS             5447..6448
                     /gene="gap"
                     /locus_tag="MBZ71_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBT4429918.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6558..7748
                     /locus_tag="MBZ71_001994"
     CDS             6558..7748
                     /locus_tag="MBZ71_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBT4429919.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7852..8607
                     /locus_tag="MBZ71_001995"
     CDS             7852..8607
                     /locus_tag="MBZ71_001995"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBT4429920.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8723..10021
                     /gene="eno"
                     /locus_tag="MBZ71_001996"
     CDS             8723..10021
                     /gene="eno"
                     /locus_tag="MBZ71_001996"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBT4429921.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHHLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3548 a         1870 c         2100 g         2614 t
ORIGIN      
        1 gtcacacctt cttttttgta tataaaaatc ccaagtaaat acataaaaaa taaaaaaatt
       61 gaaactttta gcgcaccttg tacgtcatat taagtgaggc agaaaggagt ggagaatgat
      121 ttttactgga tataagaaaa aacggcagat cgaaaaatac cgtcacctgc tgaatggact
      181 catcgaaact gattatcaaa aaatgttccg tatcacgatg aattacgtac atgataaaga
      241 agaggcctta gatgtgatgc aagatagttt tcataaagcc ctgaactatt ttgaaaaaga
      301 aaaagagatc gagcatttta gtgcatggtt ttatcggata ttgatccgca cggcattaga
      361 tagttggcgc agacaaaaaa ggacgcctgt ggacttattt gaaattcaag atatccccca
      421 acagttacct ttagaaactt ccgttgcaga gctccatatg attttagata aaatagacag
      481 tccggaaaaa gagattctga tcctctactt ttttgaagga tttcgtttga aagaaattgc
      541 gactatcttg gatatgaatg aaaacacagt taaaacgaaa atgtaccgct cattgaatgc
      601 tttacgtcaa atcctagaat gaaggagaga agagtgccat gtattcccaa aaagaaaatt
      661 taaaaaaact aaagaaaact tataaagaac aagcaattcc caaagggctt aaagaggaga
      721 tccgccagcg ttttattatc gaagagcaat catttataaa agcaaagcgg agaaagagac
      781 gtctccaagg gttcggagta tctttggcta tcgtaatgat tacaacaagt agccttcttt
      841 ttaatagtca agttcgaagc tttgcagaag atttacccat cttaggatca gtcattgaat
      901 tgatcttagg cgaacgcttt actgatcgat ctgaaaaaat cgatattcag gtaccgaaga
      961 tcaatacaca aaatgagaaa gaaaataaaa caattcatgg attaaatcaa aaatactttc
     1021 gcgaaggaca agcagatttt gaaaaagctg aaaaagaata tggaaatttt gaaacggatc
     1081 attaccaagt tttgggagat tatcagaaaa tcgtagacga taaccggttt ctcgtgattg
     1141 aaaggcagat cacccaaaca gcagcagata gccatgttga aaaaagatac gacacaatcg
     1201 acaagaaaaa cagtgtccag ctctcacttc cacttttatt caaagatgac acctatctat
     1261 ctgttttgac aaaagaagtc aaacggcaga tggccgaaca agtcaaggaa gatccatcta
     1321 agtactattg gactgagcaa gatattcaag aagggacgat agaaaaacca acacttgtta
     1381 cgcctacacg aagtttttat ttgaacaaag agcatcaatt agttttgact ttttctcaat
     1441 acgaaattgc tcctggttat atgggtacac cagaatttgt cattcctaag tccgtgacaa
     1501 aaacgatttt ggcatcggaa gattatttgg atcattagac gagaagtagt tgcgactagc
     1561 agtttcaatt ttcaagtaag tacaagtaaa taataagaag agaaaaagag aaagaagaga
     1621 tatagtgaaa agaaattatt tagttatcag tattattgct ttatccgtgc ttttgttttt
     1681 cgaaattact tcggtggcct ccttgatcac cgaagtaaaa ggacaaacac actcttcgga
     1741 gcattcgtca caatctttca aacaatcaga acaatcaacc attaaaagcc aatcaactga
     1801 aacagctaac gtcaaagaag ccattcaaga atcagcggaa aaggtacaaa gtcagtcatc
     1861 acaatcaaca caagacagta caaaaacgag ccgagatccg gctgtcaaaa gcgtcgcaat
     1921 cagctttgat gatggaccag gagcgaccac aacaccgcag cttctgcgaa tcttaaaaga
     1981 aaaaaacgtg catgccactt ttttcgtctt aggagaaaat acggcgcagc atcctgaaat
     2041 cgtgaagcag acagctgaag cagggcacga gattggcaat cacacatacg atcatcagaa
     2101 tctggccact ctttctgccc agtcgatgac ggaggaagtg acgaaagcag acactgagat
     2161 caaaaaagtc actggcaaaa cccccacttt tgttcgtccg ccctatggtt ctgtcacgaa
     2221 tgtaggagca acgatcatcc agcgaccgat catcgaatgg tctgttgatt cagaagattg
     2281 gaagacaaga aatcctgatt tgattttgca gaagatccaa gcaacagtat acgatggggc
     2341 aattatttta ttccatgata tctatcctga aacgattcgt gccgttcctc aagtgatcga
     2401 ttatctgaaa gagcaagggt atcgcatcac gacagttggg gatttattag ggcatccaac
     2461 ggctgtcgag aactattatg gcagaaacga tcatcggcca gttcagtaag tgagacaagt
     2521 atctttaata aataacacat tttaataagt agttgaacac ataactacct tacttgcaaa
     2581 ggcttaacaa tatctataca ggtacaacaa aaaactgtcc accaattttc tttggcggac
     2641 agttttcact taaaaataaa gaagcaacta ttcgtaaatt atgctaaaat agcagtagaa
     2701 cgcgtcctga gggaatcgaa cccccgtctc aagaaccgga atcttacgtg atatccacta
     2761 cactaaggac gcaagcactg ggataattgt aactaaacag ataaaaaatt acaaggggat
     2821 tggacaaaaa aatgaaattt gagcaaaatt tttcacaaaa acagcagcag acacagaaac
     2881 tagccatgac gcaacagttg caacagtcca tccaagtttt acaatataac agtgaggagc
     2941 tgttggcatt cgttgagaat caagcaatgg aaaatccatt ggtggaagtt gttgagccgg
     3001 aatggcagcc agattacata aaagcttctt catcttctta tgaaggagaa gagacaaatt
     3061 acctaaatca gattcctgat acaaaagggt ctttatttga ttcattgatc gaacaagtcc
     3121 atttgaatta ccgagatact tttttgagga aaatcgttct ttatcttgta gaatacatcg
     3181 atttgaacgg atttttgacg attagtttag aagaagccgg aaaagagatt ggagctacgc
     3241 ctatccaaat gctcgatgcg ttgactctga ttcagcaatt agaaccagct ggagtaggtg
     3301 cacgtagttt gcaagaatgt ctcatgcttc aaacagaacg agatgattat gcgccggaat
     3361 tagcttatat cgtgttggaa gaatgttttg aggaactggt ggagcgaaaa tggaaagaaa
     3421 tcgcacaacg tttttctgtt gatctacatg ctgttcagca gatattcgac tatttacaaa
     3481 cgttgagtcc aagccctggc cggattttcg accgtagcag cgaattgtac attattcctg
     3541 atgtccgggt attagtggac gacgataaaa atgtacaagt tatctcaaac cgaaaaaatc
     3601 agccaaatat acgctttcaa gagagttact tcaaacaaat gtcacaacaa gcagataaag
     3661 aaactgaaaa ttatttaaaa gaacggaaac aggaattcga atggttgaag aaaacgatcc
     3721 tacaacgtgg ggatacaatt ttgcgggttg ctcaagtcat tgtttcccgg caaaaagctt
     3781 tctttatcga caaggaacgt ccaatcaagc ctcttacgtt aaaggaagtt gcaactgaga
     3841 tagatgtgca tgaatccacg gtcagtcgtg cagtcaacgg gaaatatttg gaaacagact
     3901 tcggtgtatt tgaattaaaa aaatttttca ctacacgaat tcctacgaat agtacagaac
     3961 agacagaaga cctttcagca gatacagcga agaaaaagct gcaggaatta gtcgatcaag
     4021 aagacaaaaa caagccgttg tctgaccaga aattggttga attgctgaaa aaagatgaga
     4081 tagcgatctc aagacgaaca gtagcaaaat atcgtgattt attaggaatc ccgagttcat
     4141 caaaaagaaa acgttatgat aactaatcag tcaaaagact agaaaaagta tttcactttt
     4201 tctagtcttt ttagttgaaa aacgagagca aaactgttag acttacagtc gtgaggttga
     4261 gatttccctt tattgatgtg aaattcgaat agttaatttc tttttttatc gtatatgggt
     4321 cgttttaagt ctacattgga cgtaaagtgt ccaactaagg agatcgctat gctggatgaa
     4381 ataaaaatga ttgaatctgt tgcaccagat attatcgaag tgttgcaaga acgctataaa
     4441 attttacgaa atatctattg gatgcagccg attggacgtc gaagtttatc ggaaagtatg
     4501 gggatcacag aacgagtatt gcgaacagaa acagatcttt tgaaaaatct gaatttgatc
     4561 gacacctcaa aaagcggtat gacgctgaca actaagggcg aagaagttta tcaaagcctt
     4621 gaaaacttca tggatcagct tttaggtacg catcaaaccg aacaacagtt ggcagaatat
     4681 tttggtaacc aacgctgtat cgttgtttca ggaaacagcg aagagcagac caaagtggct
     4741 gatgcattcg gcgaagcgct gagcgaagcc ctagaccgac tacttccaga aggcgagaat
     4801 atcatcgctg tgatgggagg aactactatg gctgtggtag ccgaacaatt gtctaatctg
     4861 gaaaataaga aacggcataa cctgtttgtc cctgcaagag gcgggattgg tgaagcgata
     4921 actgtacagg caaattctgt cagtgcaaga atggctgcta aagcaaacgg taaccaccgt
     4981 gcactttatg ttccggaaca attaagtctg gcaacttaca attctttact caatgagcca
     5041 tctatccaag aagtcttaaa cttgattagt gaagcgaatt gtgttattca tagtattggg
     5101 cgtgcattac acatggcagc acgtcgtaag atgactgaaa aagaattagt catgctgaaa
     5161 caagcaaatg ctgttgcgga atcttttggc tactttttca atgaaaaagg tgaagtcgtt
     5221 tataaggtcc ctagaatcgg catagaattg caagatttgg aaaaagtccc gatcatcatg
     5281 gcaatcgcgg gcggaaaatc aaaagctaaa gcaattcgag cttatatgaa aaatgcacca
     5341 aaacaaacgt ggctcatcac tgatgaagct gctgcaaatg agattttaaa aggggtaacc
     5401 ctttaaaata aatatttttt tgattttcat aaggaggaaa tcttaaatga cagttaaagt
     5461 aggtattaat ggttttggac gtattggacg tctagcattc cgtcgtatcc aagatgtaga
     5521 tggaatcgaa gtagtagcaa tcaacgactt gacagatgct aaaatgttgg cacacttgtt
     5581 aaaatacgac acaactcaag gacgtttcaa cggaacagtt gaagttcatg aaggttcatt
     5641 caacgtaaac ggaaaagaag ttaaagtatt agctaaccgc aacccagaag aattaccatg
     5701 gggcgaacta ggcgtagata tcgttctaga atgtactggt ttcttcactt ctaaatcagc
     5761 agctgaaaaa catttaacag ctggtgctaa acgcgttgtt atctctgctc ctggcggaaa
     5821 cgatgttcca acaatcgttt ataacacaaa ccacgaaaca ttaactggta aagaaacagt
     5881 tatctcaggt gcttcatgta ctactaactg tttagctcct atggctaaaa cattgaacga
     5941 caaatttggt gttgttgaag gattaatgac aactatccac gcttacacag gtgaccaaat
     6001 gactctagac ggacctcatc ctaaaggtga cttccgccgt gcacgcgctg ctgctgcaaa
     6061 catcgttcct aactcaacag gtgctgctaa agctatcggt ttagtaattc ctgaattgaa
     6121 cggtaaatta gacggagctg ctcaacgtgt tcctgtacca acaggttcat taacagaatt
     6181 agtaacagtt cttgaaaaag aagtaactgt tgacgaaatc aatgcagcaa tgaaagaagc
     6241 ttcaaacgaa tcttacggat acaacacaga cgaaatcgtt tcttcagata tcgttggtat
     6301 gacttacggt tcattatttg atgctacaca aactaaagta atgacagttg gcgacaaaca
     6361 attagttaaa actgttgctt ggtacgacaa cgaaatgtca tacactgcac aattagtacg
     6421 tactttagaa tacttcgcta acttataaga tagacaaacg tacaagtgta aattatagac
     6481 atgtacattt aaaagcgggg aagcaatcgc gcttcttccg ctttttttga taataagttt
     6541 attcaggagg aaataaaatg gctaagaaaa cagtgaaaga tatcgattta aaagacaaaa
     6601 aagttcttgt ccgtgttgac ttcaacgttc ctttgaaaga cggcgtgatc actgacgaca
     6661 ctcgtatcaa agcagcattg ccaacaatca actacgttct agaacaaggc ggaaaagcaa
     6721 tccttttctc tcaccttgga cgtgtaaaaa cagaagaaga taaagaagga aaatctttag
     6781 ctccagttgc aaaacgtcta ggcgaattat taggtaaaga agtaacattc gtacctgaaa
     6841 ctcgcggaga acaattagaa gaagcaatcc gcaacatgaa tgacggcgac gttgtcgtat
     6901 tcgaaaatac acgatttgaa gatattgacg gtaaaaaaga aagcaaaaat gatacagaac
     6961 taggtaaata ctgggcttca ttaggcgatg tatttgttaa cgatgctttc ggtacagctc
     7021 accgtgcaca tgcttctaac gtaggtatcg cttcaactgg tatcccaaca gttgctggat
     7081 tcttgatgga aaaagaaatc aagttcatcg gtgaagcagt agaagaacca aaacgaccaa
     7141 tgattgcaat ccttggtggc gcaaaagttt ctgataaaat cggcgtaatc gaaaacttga
     7201 ttcctaaagc agataaaatc ttgatcggtg gcggaatgac ttacacattc tacgaagcaa
     7261 aaggaatcaa gatcggtaac tctctagtag aagctgataa agtggaacta gcaaaagaat
     7321 taatcgaaaa agcaggcgac aaacttgtct tgccaatcga caatgtctgt gcaccagaat
     7381 tttcaaatga tgttgaaaca caagtaatcg aaggagacat tcctgatggt ttgatggctt
     7441 tagacatcgg cccagcatca gtaaaattat ttgctgatac attacaaggt gcaaaaacag
     7501 ttgtttggaa cggacctatg ggtgtatttg aaatgagtaa ctttgctaaa ggtacaatcg
     7561 gtgtttgtga agcaatcgct aacttagaag gcgcaacaac aatcatcggt ggcggagact
     7621 cagctgcagc agcagaacaa ttaggatttg ctgacaaatt cacacacatc tcaacaggtg
     7681 gtggcgcaag cttggaattg cttgaaggta aagaattacc aggactagca gcaatcaacg
     7741 ataaataaac gaccaagttt gctcaattct gacgagtaat cgatcaaaag gtgaagggag
     7801 ttttcatgac tccctagcca tttttcaata aattaaggag tgtgttcctc aatgcgtaaa
     7861 cctattatcg caggtaactg gaaaatgaac aaaactgcat cagaagcaaa agaatttgct
     7921 gaagcagtaa aaaacaaaat cccttcaaat gacaaagttg attcagtcat cggatctcct
     7981 gcattattct tacaagaatt aatgacatca gctgaaggta cagaactaaa aatctcagca
     8041 caaaactgct actgggaaaa ctcaggtgcg ttcactggtg aaacttcacc agcagctctt
     8101 gctgatctag gtgttcatta tgtgatcatc ggacattcag aacgtcgtga atatttccat
     8161 gaaacagacg aagacatcaa taaaaaagca aaagctatct ttgcaaatgg tatgacacca
     8221 atcctatgct gcggcgaatc tttagaaacc tatgaagcag gcaaaactgc tgaatggatc
     8281 gaaggacaaa ttacagctgg tttagctgat ctttcagctg aacaagtgtc aaacatggtt
     8341 attgcttatg aaccaatctg ggcaatcggt actggtaaat cagctgacgc acaaatcgct
     8401 gacgaaattt gtggagttgt acgtcaaaca gttgaaaaac tttatggaaa agaagtatca
     8461 gaagcagttc gtatccaata cggcggttct gtaaaaccag aaaatattgc tgaatacatg
     8521 gcaaaagaaa atgttgacgg tgctttagtc ggtggcgcaa gcttgcaagc agattcattt
     8581 ttgtcattgt tagacgctgt aaaataattg ttgaaaattt gcgaaaaatg caagaaatgt
     8641 ttgcactaat gagcaaaatt aactaaaatc gtatatgagg gaaatccctt aggaaaaaac
     8701 aaactcaaag gagagacaaa acatgtcaat tattactgat gtttacgctc gcgagatctt
     8761 agactcacgt ggtaacccaa caatcgaagt agaagtatac acagaaagcg gagcttttgg
     8821 ccgcggaatg gttccttcag gtgcttcaac tggtgaatac gaagcagttg aattacgtga
     8881 cggagacaaa gctcgttatg gcggtaaagg tgtaactaaa gctgtcgaca acgtaaacaa
     8941 catcattgct gaagcaatca ttggctacga tgttcgtgac caaatggcta tcgacaaagc
     9001 tatgatcgct ttagacggaa ctcctaacaa aggtaaatta ggtgcgaacg ctattttagg
     9061 tgtttctatc gctgttgcac gtgcagctgc tgactaccta gaagtacctt tataccacca
     9121 cctaggcgga ttcaacacaa aagtattgcc aacaccaatg atgaacatca tcaacggcgg
     9181 atcacatgct gataactcaa tcgacttcca agaattcatg atcatgcctg ttggcgctcc
     9241 aacattcaaa gaagctctac gtatgggtgc tgaagtattc cacgcattag cttcaatctt
     9301 gaaagctcgc ggattagcaa cttctgttgg tgacgaaggt ggatttgctc caaaccttgg
     9361 ttcaaacgaa gaaggttttg aagtaatcat cgaagcaatc gaaaaagctg gctatgtacc
     9421 tggtaaagac gttgttcttg ctatggatgc tgcttcttca gaattctacg acaaagaaaa
     9481 aggtgtttac gtactagctg actcaggcga aggcgaaaaa acaactgacg aaatgatcaa
     9541 attctacgaa gaattagttt ctaaatatcc aatcatctca atcgaagatg gattagacga
     9601 aaacgactgg gacggattca agaaattaac tgacgtatta ggcgacaaag ttcaattagt
     9661 tggtgacgac ttgttcgtaa caaacactca aaaattatct gaaggtatcg aaaaaggaat
     9721 tgctaactca atccttatca aagtgaacca aatcggtaca ttaacagaaa ctttcgaagc
     9781 tatcgaaatg gctaaagaag ctggctacac agcagttgta tctcaccgtt ctggtgaaac
     9841 agaagattca acaatctctg atatcgctgt tgcaacaaat gctggccaaa tcaaaactgg
     9901 ttctctatca cgtactgacc gtattgctaa atacaaccaa ttactacgta tcgaagacca
     9961 acttggtgaa gttgcagaat acaaaggttt gaaatctttc tacaacttaa aaaacaaata
    10021 attcttttct aatgaagata catgatcgac tattgaaaga tagtcgagta tgatcactta
    10081 gttaagtaat cgaactctct acttgctgaa atatcagcag gtagagagtt tt
//
LOCUS       DAGCWU010000106        10116 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.106, whole genome shotgun sequence.
ACCESSION   DAGCWU010000106 DAGCWU010000000
VERSION     DAGCWU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.106"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(133..1713)
                     /locus_tag="MBZ71_001997"
     CDS             complement(133..1713)
                     /locus_tag="MBZ71_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBT4429922.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2114..3490)
                     /locus_tag="MBZ71_001998"
     CDS             complement(2114..3490)
                     /locus_tag="MBZ71_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBT4429923.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3618..4736)
                     /locus_tag="MBZ71_001999"
     CDS             complement(3618..4736)
                     /locus_tag="MBZ71_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4429924.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4878..5158
                     /locus_tag="MBZ71_002000"
                     /pseudo
     CDS             4878..5158
                     /locus_tag="MBZ71_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(5236..5769)
                     /locus_tag="MBZ71_002001"
     CDS             complement(5236..5769)
                     /locus_tag="MBZ71_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBT4429925.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(5863..7041)
                     /gene="mutY"
                     /locus_tag="MBZ71_002002"
     CDS             complement(5863..7041)
                     /gene="mutY"
                     /locus_tag="MBZ71_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBT4429926.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7034..7831)
                     /gene="recX"
                     /locus_tag="MBZ71_002003"
     CDS             complement(7034..7831)
                     /gene="recX"
                     /locus_tag="MBZ71_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBT4429927.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            7938..9320
                     /gene="rlmD"
                     /locus_tag="MBZ71_002004"
     CDS             7938..9320
                     /gene="rlmD"
                     /locus_tag="MBZ71_002004"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBT4429928.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9494..10072)
                     /locus_tag="MBZ71_002005"
     CDS             complement(9494..10072)
                     /locus_tag="MBZ71_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBT4429929.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         2980 a         2082 c         1747 g         3307 t
ORIGIN      
        1 atttctatga ttttaatata gtaaaaagag ccgtcacgga aaatccgtca cggctctaaa
       61 caaaatatga gagactgttt ttgtttggct attcttatta cttgatgcag ggcagcttgt
      121 gtcaagactt ctttacatca aactttttaa ttttacatta ggatatttat ccgcaaacca
      181 gcgttctgca aattggttct cgaataagaa caatggttgg tcgaaacggt cacgagccaa
      241 gatattgcga cttgaactca tcttctcatc caaatcttct ggatcgatcc agcgagcgat
      301 tttgtgtccc attggagaca tgattacttc ggcgttatac tcatttaaca tacgatattg
      361 gaatacttcg aattgtagct gaccaacagc tccaatgata tattcttccg ttagatatgt
      421 tttataaagc tgaatcgctc cttcttgaac taattgatag attcctttgt ggaaggattt
      481 ttgtttcatg acattttttg cagtcacttt cataaacagc tctggagtaa atgaagggag
      541 ttcttcataa gcaactttta gttttccttc gaacaatgta tccccaatct gataattgcc
      601 tgtatcatat acaccgatga tatcgcctgc tactgctttt tcgacgtttt ctctcgaatc
      661 tgccataaat tgtgtgacat tgcttaattt cagtttttta tttgtacgtt ccaaccagac
      721 atccattcct cgttcgaatg ttccagaaca gattcggaca aaagcgattc ggtcacgatg
      781 ggctggattc atatttgctt ggatcttgaa tacgaaacct gaaaattctt cctcataagg
      841 acttaccatc tgatcatctt gtgttttgtg tccataagga gctggtgcca attcaacgaa
      901 agtctctaaa aaagtctgta caccgaagtt agtcaatgca gaaccaaaaa atactggtgt
      961 ctgatcgcca cgagcaattt tttcttgatt aaatgcatct ccagcctctt tgattagttc
     1021 tacttcttct aagacttgtt catataagga attttcagtt agaggatgat tttcgggaag
     1081 cagtccatct tcatccaatt ctactaatcg ttcatcctga taattttccg gacgataaaa
     1141 ctctacacga ttatggtaga tatcataaag accttctagc cctttaccca taccgattgg
     1201 ccaattcatc ggataagatt cgatatctaa tagctcttct aattcttcta acaattccaa
     1261 tggttcacga ccatcacgat ctagtttgtt gataaaagta aatatcggga tgccacgttt
     1321 ttttacaact tggaataatt tctttgtctg tgcttcgatc ccttttgcac tatcgattac
     1381 catgaccgca ctatccacgg ccatcaaagt acgataagta tcttcagaaa aatcttcatg
     1441 tcctggtgta tccaagatat ttactcgttt cccttgataa tcaaactgca taacagaact
     1501 agtaacagaa atcccacgtt gtttctcgat ttccatccag tctgatttgg caaaattacc
     1561 tgtttttttg ccttttacgg tcccggcttg acggatagcc ccaccaaaca atagtaattg
     1621 ttctgtaatc gttgttttcc cagcatccgg atgggaaatg atcgcaaaag ttctgcgatt
     1681 gtccacttgt tgctttaatt ctggattatt cataacctaa ctccatttct tccattcaat
     1741 tatttatatt ttctctaggc acagattgtc cgatctctta aagaaagaca atttcatata
     1801 gggctgtgac aaaagccgtg tagcagtctc tttttccgag taaacggcgt tctagagaca
     1861 gcctatcagc aataagccca cccaagccaa aaatacgaaa agctgttttt gcttggctgc
     1921 tcttattact taatgcagga tagctttttc acaacctttg tcaactacta caactgaatc
     1981 agtatagcat ggattcatcg aatgaaaaag ccaccaaacc ataagagtca aatatttttt
     2041 tgtcagctga ctaaaagaaa agacgtgttg cttgtttatt caagcaacac gtctttcata
     2101 accaagtttc caattatctt cttggctcgt catcttcgaa ttcttttctg aagtatcttc
     2161 gttcttcttc aggttccgct tctgtttctt ccggatcatg aaaaatgaca cggattttaa
     2221 ggattcgaga accttccatt tcttcagaaa ccagcgtaat attatcgaca tcaaacgata
     2281 acttttcgcc ttcatccgga atcatgccta atgctgtaat caaataacca gccatcgtat
     2341 ctacatcact catatgcaga tcactgtcaa aagcttcatt gaactcatca atcagcatac
     2401 gtccttggat gatatattcg tgttcatcga ttttttcata aagattctca acttcatctg
     2461 attcatcatc gatttctccg acgatttctt ctaaaaggtc ttctagtgtt gctaacccaa
     2521 cgacgccgcc atattcatct aataaaatag ccatctgatt ctgcgtcttt ttcatttcat
     2581 ataacagatc atcaataaag accgtttcag gaacgaataa aggctcctgc atgattttct
     2641 tgatatctaa attatcaaaa ccgaatttat gggcagcttt tagtaaattc tttgtatgaa
     2701 ggattccgac tactttatct ttgtcttcgt tataaacagg gattcttgaa tagttttcgg
     2761 ataaaacttc attcacattt tcttccacag tgtcgttgat atcaatcata aatgcatccg
     2821 ttcgtgggac catgacttct cgggcaactt tcgtatcaag agaaaaaacc ccttggagca
     2881 tttctaattc ttcattattc aatacgcctt cgttttcaag catataacgc atttcatctc
     2941 tggtcatttt tgaatcttca tcatcaaaag tcatcggcgt gatacgcgcc aataaacttg
     3001 tggaagcaga aagcagccag acgaagggct tagctactac accaatcaag cgtatcagcc
     3061 ctgaagtgaa ctttgcgact tcttctgatt tatttaaagc aatacgttta gggtacaatt
     3121 ctccaaaaac aatcgataca taggtcaaaa tagccagcac aatgatattt gcaatatttt
     3181 ttgccgctgt gccgccacct aataccggag ctaaccgttc cgataaagta ttagctagtg
     3241 aagcaccaga caaaatgttg accaaagtga ttcctacttg gattgtcgat aggaaattat
     3301 ttggattctg caatattttt aaaagctttt gagatttcac atctccttca tcagcttttt
     3361 gctcgattcg attcttattg atcgaaacaa cagcaatttc ggaagctgct aaaaaagcat
     3421 tgatcaatgt caaaacgatc aataataaaa tctgcgctaa cagcgactga ctctcagggt
     3481 cagcattcat aatcgcattc tctccttata aaattaattt ttagtttacg atagaaaaaa
     3541 acgtggcact atagccacgt taaaatggta tcacaaagca attttctttg caataaaaac
     3601 aagtgtacta aaactaccta cgaggtcgta gcaattttct tcttgttttc attttgcttg
     3661 tcttctttga cgattttgat tatataggaa atgatcgtct tgcagattgc gtaaaaagga
     3721 acacctaaga agataccaag caatccggca atatttccag ctactagaag aacgataatg
     3781 atcgttaatg gatggatttt cagtgatttc ccaattacat taggataaat gatattccca
     3841 tccaactgtt ggacgattag cacaaccaca caacaaagta atgcttttac cggatcattg
     3901 aagactgtta ctagaaatgc aggtgctagt cctagataag gtcctaaata tggaattagg
     3961 tttgtcaatc ccgcaatgac cccaaataag aaagcataat ccacaccaat taccatatat
     4021 cctaaaaatg tgaaggtacc aacgaataaa cattcgattg cttgtccgct aatgtaatta
     4081 gccaatgttt tattcagttg ccccaataac tccacaattt ggtttcttct tttttctggg
     4141 aaaaactgtt ggatgtttgg cactagtttt tctccgtctt tcaacatata gaacaagatg
     4201 aatggtgctg taatgattac tacggttgcc gaagcgatcg ttccaacgat cgaacctaaa
     4261 ctattggata ctccactaag gaattgctga atgattgtcc cgtaagaaat gtctaattgc
     4321 tcaaaatatt tattgatatc tacttccttg aacaaaggca attcggctac ctcttttaac
     4381 cagctttcta cctgtttgat gaaatcaggc atattagatg ccaatgagga aatttgctgt
     4441 actagacttg ggatgacact aagtaaaatc caaataattg ctgcaactaa aaggagcagc
     4501 acaattgcga cagcccatat tcttttgatt tttgctttca tcaacagctt gacaatagga
     4561 ttcaataaat agtaaagaaa gcctgcaatc agtacaggtg caaataacgt ggaaaaaaat
     4621 gtcccgatcg gttcgaaaat aaagtttatt tctgatgaaa tcatgatcaa tgcagctatg
     4681 atcaataatt caagcgacca aaacattagt ttagactgtc taagtttttc aaacaataga
     4741 tttcctcctt tctttcttac tagtatatat tatattcgtt aaaaattctt ttattctttt
     4801 tttatttttg aaaacttgcc atacttttta tttgttttat tttccagtat actatacgaa
     4861 aggaaggtgg ctagaccatg aaagttgtac aaacaaaaga tacaatgagc aatatttact
     4921 tagatgctgt acggatcagg catcaagttt ttgtagtcga acagggtatg cctttgtctc
     4981 gagaaataga caaagatgaa gcccattgta tccacttcgt cctgtactca gataaaaaag
     5041 aaccgcaagg aactgtacgt cttttgcctt tagaaaacgg aaaaatgaaa ttacagcgga
     5101 tggcgatcct tttgaagatg ctggcatgcc tcatatttat atgaaaaaga ctttataaaa
     5161 atttatttcg aaaaaagcaa aaaacagcaa gcttttgaga aaaatctctc actggcttac
     5221 tgttcttttt tgtgtttact tacgcgacaa ttgcttgtaa cggttatacc aaatatcaat
     5281 atagccttcc gagaacgggc catctccatt attgatccag tcaaccagaa tcttcacatt
     5341 ttcttttagg atgaagtcaa tatcgttcgg ataattcatc attttgctgt gcatctcata
     5401 ttcatcgaca tccaatagtc gtttctcgcc gtcaggaaat accttgatat ccaagtcata
     5461 gtcgatatat ttcaaggcct catcgtccag caaaaaaggt gaagctagat tacaataata
     5521 ggaaaccccc ttttctctga tcattgctat tacattgaac cagtattttt tatgaaaata
     5581 aacaatcgct ggttcgcgcg ttacccatcg gcgtccatca gattcagtga ctaatgtgtg
     5641 atcattcacg ccgatcatag agtgttcgct tgtttttaat accatggtat ctcgccacgt
     5701 tcgatgcaat ttgccgtcgt gcttgtaact ttggatcgtc acaaactctc cttcttttgg
     5761 aactcccata gcaacccttc cttaagcagc attttgtgct ttgtgtttgg gtgttcatta
     5821 caccgacatc ataattatta tatcaataaa aatgagcttt gcctagaaag gattcctatt
     5881 gtttcgattt ttctctaact gatctaccag tttcatttgg accttaggaa aaacgaccga
     5941 atcaaatgca tcaggcttca accacttcga agttttgttc tcggtaaatt cttgttctgc
     6001 tccctcggtt gcccgtccgt aaaaaaggag aacatgccat ttcagatgac taaaaatatg
     6061 agtaacttct cctaaatgtc gtgtctgcca aacaaagagt tcctgctttt caaatgggag
     6121 attttgctct tcttctgcta ctaggtcatc aaacagatcg atttctggct ttgtttccca
     6181 ttcttttttc aatcgctcat actcctcttg tgtcacttcc atcattggaa atgtccacat
     6241 attcgccagc aatttttggc tatctctttc ttcaaaataa tacgctccag aatgattttg
     6301 aagagccgca ctgatataat aaacgtcttt tggctttgct ttttttgttt ttacaggaaa
     6361 actcgtttgg attcctcgtt tgttagctaa acaaaaggct tggatcggac aagtctcaca
     6421 ctttggagaa gttggtgtac agatagctga acctagatcc atcattgctt ggttgaattc
     6481 acctggatgc ttttcatcaa tgattttccg catcgcttca tcaaagattt tcctgctaga
     6541 agcttttgcg atgtctgctt caatgcaaaa caatctgctg actactcgca tcacgttgcc
     6601 gtcaaccgcg ggttctggaa gtccaaatgc gatacttgca atcgcgcctg tcgtatatgg
     6661 tcctattccc ttcaatgagc taatttcttc aggcgtttgc ggcatttccc catcaaattc
     6721 tgacatgatc tgtttagcag cagcttggat gttccgggct cttgagtaat aaccaaggcc
     6781 ctcccatgct tttaaaagct tttcttctgg agcatttgct agctcttcaa ttgtaggaaa
     6841 ccattccatg aaacgataaa aataatcgat cactgtgtct actctggttt gctgaagcat
     6901 gatctcagag atccagatac gataaggatc acgattatag cgccaaggca ggtttctttt
     6961 ttcttgttca taccattgaa tgaactgatc ttgaaattct tttgtttctt tatctgtcca
     7021 tttttcccat tctttattca ctttcctctt ccttttccgt aatgaatcgc tggatcatat
     7081 ccaaatcaaa tcctttttga tataagcttt gctttacttt tagatttctt tttttaggtt
     7141 caaaccttaa atttcttcgc catagtttat ctccttgttt gactaaataa tcatattcta
     7201 tttcttgatc tgcttcttgc ggaagatcag ccatcgcttg ttggatcact tcttgagaaa
     7261 aacctttcgt catcaatgtt tgttggattt ttctcaataa ttctttttgg ctttttccgc
     7321 gattttttcc aaacgctttt tcggctgtct gatgtgcatt ttgaacttgg tcttcaaatg
     7381 tatattggct gagcgcttgt tcaatgattt caggtttcaa acctttttgc tgcatctgct
     7441 gcgctaattt ctttggtcct ttgtcactta agcgcatatt cgtgcgcaca taactttccc
     7501 catagaccaa atcatcgaca aggtccaatt cttttaagcg ggctacgatt ttatttcgat
     7561 cttcttgaag gatttccttt tcttttaagt atgttcggat ttctttttcc gatcgcaact
     7621 gataacttaa gtaattcatt gctaggtgaa aaccgaaatc agctccgcta ctcttcttga
     7681 tctcttggat agtttcttcg tctaattccc gtccttttaa taatcgatag cgtactaata
     7741 catcttcaga tacttgtaaa gcttctcctt cagataaatc tacgcgatag aaagggcctt
     7801 tgcctttact gattctcgta acggttaaca taaacatcct cctcgaacat atattctcta
     7861 tcataccaca gaaaatcccc gtaatcaggt attatatgtt agaataaacc acagaaaaaa
     7921 gaaaaaggga gatacttatg gcagaaacaa ttgtcaaaac tggacaaacg gttcctttga
     7981 aaatcaagcg tttaggcata aatggtgaag gaatcggtta tttcaaacgg ttgatcatat
     8041 ttgttccata tgcactacca aaagaagaag tactagtgaa aatcacgaaa gcaactcctc
     8101 gctatgcaga aggagaatta ataaaagtca aaaaaacaag caaagatcgc gtagtggcac
     8161 catgtcctgt ttactatgaa tgcggtggct gtcaattaca acacttagct tatcaagctc
     8221 aattggattt taaaaaggat ctgcttttgc aagcattgga aaaattcaaa ccagcaggct
     8281 ttcgatctta tcaacttttg ccgacgatcg ggatggatga gccttggcat tatagaaaca
     8341 aagcacaatt tcagttacga aaaaataagc agactcaaaa aattgaagct ggtttatacg
     8401 aagcaaactc tcatcagtta gtccctataa cagactgctt ggttcaagaa cctcgaacac
     8461 aaaaagtaat gaatactgtt gttcaactat tgaataaatt tgatgcaccc atctatgatg
     8521 aacggacaaa cagtggaatc ttccgcacaa tcatggttag aatcggtgtt aaaactgggg
     8581 agttacaagt tgtttttatt actcgcagta aaaaattccc gcaaaaaaac gcaatgatct
     8641 gtgagatcaa tgaacgactg cccgaagttg tttcaatcat gcaaaatgtc caggcacaaa
     8701 aaacgtctct cgtgatgggg gatgaaacga tccacttatg gggaaaagaa gccattgaag
     8761 aacaaatcaa tgaagtgact ttcgatcttt ctcctcgtgc cttctttcaa ctcaatcctc
     8821 agcagaccga attgctttat ggcgaaggaa tcaaagcatt agatgtccag cctaatgaaa
     8881 cgatcgtgga tgcttattgc ggagttggga cgatcggctt aagtgtagca aaacaagcca
     8941 aagaagtacg gggaatggat accgttcctc aagcaatcgc tgatgcaaaa aaaaatgcgg
     9001 aaagattagg atatcacaac actcgatatg aagtcgggac agcagagcaa gttctgccta
     9061 aatggttgaa tgaaggtttt cagcctgatg gtatcatcgt agatccacca cgtacgggtt
     9121 tggataacgc attgatccaa tcattattga aataccctcc aaaaaaactt gtttatatct
     9181 cctgtaatgt ttctacactt gccagagatc tggttaattt aagtaaaaag ttcaagattg
     9241 aatatttaca gtctgtcgac atgtttcctc agactgcacg ttgtgaagtt gtcgtgaaga
     9301 tgacgagact cgctgactaa atgaaagagg ttgtgaaaaa gtctgccctg tatcaagtaa
     9361 taagaacagc caaacaaaaa tagctcttca tgttttatga ataccgtact tgtgaccgaa
     9421 gagttaacag gaggacacta tttatttaaa aaaagggttg tgacatagtt tttgtcacag
     9481 ccctttttga ttattattgg atattcacgt ctatcacttg ataaaatgca tttgtcgtat
     9541 cgctgatatt ccatactgct aaaatcacat tatatccttt tcgatcctgc ggaagtgtga
     9601 tcgtttggtt gactgttttt ggtggaacag ttgctttgtc gtctattttt gtgatcaatt
     9661 caaaatcact aaatttcaaa ggttgattag gattccaagt tggtttggta atgtagtaat
     9721 cccaagtact tgtccgatgt tgtgctgtta aattccaagt aatatttaat gttcctggat
     9781 taaccatgtt tttatgccat ctgttagatg tctgttcatc taaaggctca aagccgctga
     9841 ctcctgcact agcaattttt ccatcaatga atgtattttt ggtagcttcg atactttgcg
     9901 gttcccattg tgctcttccg acattttgat ttaagtttcc tgctgctgat gttcctaggt
     9961 atgcacggct tcctggagaa gtaatataac catgcgcaaa acctggaatg ctaaaaccga
    10021 acaaccctaa actaactaac ccaataaaca ataacgtact aatctttttc atgtagaaac
    10081 ctccttcatt tgataaccac attgtaaacg ctttat
//
LOCUS       DAGCWU010000107         9949 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.107, whole genome shotgun sequence.
ACCESSION   DAGCWU010000107 DAGCWU010000000
VERSION     DAGCWU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.107"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..838)
                     /locus_tag="MBZ71_002006"
     CDS             complement(<1..838)
                     /locus_tag="MBZ71_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBT4429930.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GIT"
     gene            984..1787
                     /locus_tag="MBZ71_002007"
     CDS             984..1787
                     /locus_tag="MBZ71_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBT4429931.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1965..2912
                     /locus_tag="MBZ71_002008"
     CDS             1965..2912
                     /locus_tag="MBZ71_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBT4429932.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2980..3273)
                     /locus_tag="MBZ71_002009"
     CDS             complement(2980..3273)
                     /locus_tag="MBZ71_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBT4429933.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3301..3966)
                     /locus_tag="MBZ71_002010"
     CDS             complement(3301..3966)
                     /locus_tag="MBZ71_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBT4429934.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4106..4738)
                     /locus_tag="MBZ71_002011"
     CDS             complement(4106..4738)
                     /locus_tag="MBZ71_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4429935.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4745..5812)
                     /locus_tag="MBZ71_002012"
     CDS             complement(4745..5812)
                     /locus_tag="MBZ71_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4429936.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(5809..6540)
                     /locus_tag="MBZ71_002013"
     CDS             complement(5809..6540)
                     /locus_tag="MBZ71_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBT4429937.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(6708..7187)
                     /gene="greA"
                     /locus_tag="MBZ71_002014"
     CDS             complement(6708..7187)
                     /gene="greA"
                     /locus_tag="MBZ71_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBT4429938.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7323..8498)
                     /gene="mltG"
                     /locus_tag="MBZ71_002015"
     CDS             complement(7323..8498)
                     /gene="mltG"
                     /locus_tag="MBZ71_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBT4429939.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            8663..9823
                     /locus_tag="MBZ71_002016"
     CDS             8663..9823
                     /locus_tag="MBZ71_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBT4429940.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT         3017 a         2034 c         1556 g         3342 t
ORIGIN      
        1 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
       61 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
      121 actgccaaat atggacatga tgaacattct ttaccccatc gattgcttca atttcttgtt
      181 ccattttttc atagtcaata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
      241 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
      301 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
      361 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
      421 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
      481 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
      541 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
      601 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
      661 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
      721 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
      781 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
      841 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
      901 cagatgtttc cctaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
      961 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
     1021 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
     1081 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaata
     1141 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
     1201 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
     1261 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
     1321 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
     1381 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
     1441 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
     1501 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
     1561 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
     1621 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
     1681 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
     1741 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
     1801 ctcgttattt ctacttaaat agaccctttt agaggttgtg acattttttt gtcacagccc
     1861 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
     1921 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
     1981 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
     2041 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
     2101 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
     2161 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
     2221 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
     2281 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
     2341 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
     2401 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
     2461 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
     2521 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
     2581 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
     2641 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
     2701 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
     2761 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
     2821 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
     2881 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
     2941 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
     3001 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
     3061 ttcatctgct tcctcgataa aaaaaagtgt cccatccatt tccactttct ttacaggatg
     3121 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
     3181 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
     3241 tttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
     3301 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
     3361 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
     3421 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
     3481 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacag
     3541 ctctaagtag tctagaatat tctttgatac taagttatgt gcaatccgta tccccatgtc
     3601 tcgctcaggg tgtacgacac gatctgctcc gatcttctct agtacacggg catggtactc
     3661 attttgggct tttgctaaaa tattcggtac gcccatttcc tttaccatca gagtaactaa
     3721 gatgctggct tggatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatacc
     3781 taatgaacga agtgtcatct catcctgtgc atttgccaca acagcatggg tggctatatt
     3841 catatattca ttgactcgat cttcattact gtcaatagcc agtacttctt gtccagcttc
     3901 gatcagtgta cggcagatgc ttccgccaaa tcgtccaagg cctacaattg caaaattttg
     3961 tttcaccatt ttcctccttt agtatcttct tttctcagta taacaaagcc tttccaaaaa
     4021 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
     4081 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttaaac gcatagatag
     4141 ctgcttgagt tcggtcttct acctctaact tagctaatat attcgacaca tgcgttttga
     4201 ctgttttcag tgtgataaac aattcatccg cgatttcctg attacttttc ccttcagaaa
     4261 tcagcatcaa aatttcattt tctcgattcg ttaattcttc atgcaatact ggatcatttc
     4321 gccgactcat tttttccatc atttttgttg tcacttcagg ttctagtact cgctcgccgc
     4381 gttgcgtagc ccttatagca tcagctattt catgtgctgt agacgtcttc aacaaatatc
     4441 ctgctgctcc tgcttcaatt gcaggataaa ctttttcatc atcaataaaa cttgtgacga
     4501 taatgatttt ggcttttggc caatctttta agattgcttt tgttgattcg atgccatcca
     4561 tttcttccat gactagatcc attaaaatga catccggtcg aagtgccatc gccttttcat
     4621 agccttgacg tccattttca gcttctccaa tcacttcaat gtcttcttgg atagataaat
     4681 aagaagagac gcctaagcgt accatttcat ggtcatctac taataaaact tttatcatct
     4741 ttatctatgc ctccttcatc aaagggacct tggtctccac gctcgtacct tgccctttaa
     4801 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
     4861 cataacttcc cgttttggtt tcattcatat taaatcctgt cccgtcgtca atgatacgta
     4921 ataaaagatt gttatctatt ttatgcaaat agacttctaa ttcattagcc ttagcatgtc
     4981 ttaatgtgtt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
     5041 ttaattttac atcttctacc tcccatttta gcgaaatctg gattttattc tgcaattcct
     5101 tcaataactg ctcaatgcct tgttttaaac ttttttcttc taaattgact ggacgcagat
     5161 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgcc accattttca
     5221 actgcttacg gaacatttct ggtgtctcac ttttttctgc ttgctccgtc aatgccgaca
     5281 tcatcatcat cgctgcaaat aattgctgtg aaactgaatc atgaagctca cgagctaatc
     5341 gatgcctttc taattttaaa atctcttctt tggtttgtcc atctacatat tgaggacggc
     5401 tagtcataat ctgtaattca ctggatactt cgatcatttt ttcttttatt ttagtaattt
     5461 ccatatcaat atctttgatg tacagatcat ccgacgcgct tgggatcggc tgatgcaaaa
     5521 gtttggattc ataattgcca gaagaaagtg cacgaagctt ttcttcgatt ttcccgtatt
     5581 ttgccttttg aataagtgat aacaatagaa atacgatcaa tcctacacct aatgaaattg
     5641 ccatcaaatg gaagataagc ggaacgtata ataaacgcgc acgcagcaat tctccccacc
     5701 aatgactttg gttactggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
     5761 ccgcaatccc cgaataaaca gctagcattg ctttggatat tcttcccatc atattcgtat
     5821 cacctcgaca tctccaacca aagtattggt aatgatcttc aatctgcgag gattttcatc
     5881 ataatcactg ctgcagattt tgatctgttc gttttttaag gaaaactgct cttcttcaaa
     5941 caagacattt cctactaatg tcgcatgctc taaacgaata gccactccca ggggtactaa
     6001 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
     6061 tgtattgccc aaatcgatga tagtatcacc agaaatgatg gcaatgttga tatcatccca
     6121 ttcatagacg tcgtttccaa tacgttggtt accaaataat tgctgtcttt tacgctcatt
     6181 attatgtgtt tttaattgct ctgtttggac catcatgatc tgtttttttc gccaaaaagc
     6241 attttttgtc aaatctattc ctgaaatctc tacacctttc agcccgataa ataaaacggc
     6301 caatacaacc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaagct
     6361 ccctaagatc aattgaaaat tttgaaattt agtacgagga tatttgcgca tagctagata
     6421 tatattgaaa ataccgaata ttactaaaag caaaagtcca gtattgttta cgatttgcca
     6481 aacagcaaat aacagcagca gtgcttcgac taccacaaaa aaacgccaag aactattcat
     6541 cgctttttct ccctcctgac acattctcat ttcatcatac ctattttttt gactgttggc
     6601 tataggactt tagatgtaag atcgttcagt gaaacctgca atactcaaag agaaaagatc
     6661 tagagtgcgt catcagtaag atgaactctc tagatctttc agtcagacta cgcttgttct
     6721 actttgacga ttttaactaa catatcgcca cctggtgtag caatggttac ttcatcattt
     6781 ttacgtttgc caatcaatgc ttgcgcgata ggagaatcat tggaaatctt tccagaaaac
     6841 gggtctgctt cagcacttcc gacaatcgtg tactcttctt catctccatc aggcaactcg
     6901 atgaacgtaa ctgttttacc gatcgatacc tcatctgcat ctacgccgtc attatcgatg
     6961 atttgtgcaa aacggatcat attttcaatt gttgtgattc ttccttcaac aaatgcttgt
     7021 tcatctttag ctgattcgta ttcggagttt tcggaaagat ctccgaaacc gcgagcgatt
     7081 ttgattcgtt caatgatttc tccgcgttta actgttttta gttcttctaa ttctttttct
     7141 aatttttctt taccttctag agtcattgga taaactttct ctaccatgtg taacgtctcc
     7201 ttttttcgat aaaataccgc aaaaggtaat cttttgagga atatagtcca aaaaagatta
     7261 ccacgctttt cttaaaatgt aaatgttttt ctaaaaatga tcaatgacta gcccctgttt
     7321 ttctaagaat tatttacata tttttgcact agttccatat gttcttcata cgttttagcg
     7381 aaataaacat ttccagtgct cgtatctgct acgaaataat aatagtcatt ttctgctggt
     7441 tctaatactg ccttgattgc ctcttcgctt ggattgtcaa aaggaccagg cccatagccg
     7501 gtattggtat acaaattgta tggtgaatct acagctgtat cttcatacgt caccaattct
     7561 ttatgttctc ctaatgcata taaaacagag atatctgact gaagtggcat atttgccttg
     7621 attcggttaa agaagacttg tgcaatattt ttacgatcat tttcttttac gccttctttt
     7681 tcaactagag aagccaaagt taaaacttct tgtacagtca aatttttctg tttgatagct
     7741 gaataatagt tttgcatgac cgtatttgtt ttgttcacca tctcaatcac aagatcctta
     7801 agcgaagtac tcgtgtagta atcataagta gctgggaata agtagccttc caaacggtat
     7861 tttgtgttct cagctttaga ggcactttcc aacaattctg ggaatttttg atacaactcg
     7921 ttaaagaaag tctcatcatt tactaaatct aaaaattctt tcttgtcttt accagttacc
     7981 ttcgctaccc gctcagcaat ctgatcgatg tcgtagcctt ctggaatggc aattttccca
     8041 tctgcaactt ttgttggctc agaagtgccg ccttcttgca gctgtttgcc aatctcgtca
     8101 agagtcatat taggcgctaa ctgataatag ccagcttgaa agccagttaa atttttaaat
     8161 tttgtataat aattgaaaac aatcccgctt ttaataatat tgtccttctc caaaatctca
     8221 ccaatctgct tgttagaaga gccactcggg atctccactt ggactaattg gtcatctgat
     8281 ttatctaaag gcttcaaccc gctatctaca taacgataca cggtaaatcc aagaaatccg
     8341 ccaataatta ataatgtaag agcaatcact aaaatgatct tcctcacaat cttatcttct
     8401 tttttacgca gatttctccg cttgtcgatt atatctgctg tagctgcctt actggcttct
     8461 tttttatctt tacttgggtg ttgtttgttt cctgacaaaa caactcctcc ttactatcat
     8521 cttcgtaact ctgtttatta tacatgaaat ttctactgcc gtgaattatt ttaccaaata
     8581 ttatgtcaaa attgtaagtg gcaaatactc tttttgagga ttacaatgaa aaatagttaa
     8641 aaatggatag aaaggaatta acatgtcaaa aggaaaacat cgaaaaaaac aaaaaatttc
     8701 tatttctttt cttgtaattt tattattgtc tgtcagcttc ttttcaacag gcatcattat
     8761 aggaggtcaa ttaacaaaaa tacctcattc ccaaacttat aaagcggagc caacacaact
     8821 tacttccaat ttaacgcccg atgagcagag gcttgctagc aaaattgatc aattactaca
     8881 aaaatcagat tatatcggtt cgatttatgt tcgcaaaaat aatcgcgtac tattagaaaa
     8941 aggatatgga tatgcaaatc tgcgcgtaaa agaatcgaat tcaccttcta tttactatca
     9001 aatcggttcg attcaaaaag caatgactgc tttactgata ctaaaacaaa tccaagctgg
     9061 taagttaact tttgacacaa agttagcaga attttatcct cagattcctg gaagtcagca
     9121 aatcacaatc aaaaatatgc tttatatgcg ctctggtctt catcgaactg cttctcccga
     9181 caaacctatg tcggatagtg aaatcattca gttcgccctt catcatttga aacttactga
     9241 ttatgatacg tatcattatg aaccattgaa ttatacattg cttacaggga tattgatcaa
     9301 actaacgaat cagccttatg aaactcttct aaaaaaagaa atcattcgtc cactccatct
     9361 agaacataca gatttttatg acaatgtaaa aaattcgcct caacatgctg tatcttacca
     9421 gatgtctgca actgacgatt attcaaaagc attgatcgag cctgaaaccg acatccgaaa
     9481 tgaattaggt acaggaaaca taagtatgtc tgtttatgac ttgaacaaat tctttaccag
     9541 tgttctttct ggcggtatca taccaaaaga actacttttc tcattgtggc aaaataatgg
     9601 tgataaacat ccttacagcg gcggtgttta cagtggaaac gattatatcc ttgctcaggg
     9661 gaatatcaat cgattccatg cagtagtggc gatgaaaaaa gacactaaag atgcaatcgt
     9721 catggaaagt aatatccaat cggataagaa catcaaattg ccggccactg atttaagaaa
     9781 tcagatttat gaactgatgg aaggtgttca cttatcaaat taaattttga cataatatta
     9841 gttgtcatct ctgtatagtc aatttagtta gtactctatc tgaaaagtag aaataaatta
     9901 acaaatatta tctgtcctta ttatttgtta atttatttct tattttact
//
LOCUS       DAGCWU010000108         9812 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.108, whole genome shotgun sequence.
ACCESSION   DAGCWU010000108 DAGCWU010000000
VERSION     DAGCWU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.108"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(33..2153)
                     /locus_tag="MBZ71_002017"
     CDS             complement(33..2153)
                     /locus_tag="MBZ71_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBT4429941.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2383..3561
                     /locus_tag="MBZ71_002018"
     CDS             2383..3561
                     /locus_tag="MBZ71_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBT4429942.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3577..4335
                     /gene="aroD"
                     /locus_tag="MBZ71_002019"
     CDS             3577..4335
                     /gene="aroD"
                     /locus_tag="MBZ71_002019"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBT4429943.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4358..4843
                     /gene="ybaK"
                     /locus_tag="MBZ71_002020"
     CDS             4358..4843
                     /gene="ybaK"
                     /locus_tag="MBZ71_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBT4429944.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4914..6167)
                     /locus_tag="MBZ71_002021"
     CDS             complement(4914..6167)
                     /locus_tag="MBZ71_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4429945.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6284..7633
                     /gene="gdhA"
                     /locus_tag="MBZ71_002022"
     CDS             6284..7633
                     /gene="gdhA"
                     /locus_tag="MBZ71_002022"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBT4429946.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7745..9091
                     /locus_tag="MBZ71_002023"
     CDS             7745..9091
                     /locus_tag="MBZ71_002023"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBT4429947.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9399..9785)
                     /locus_tag="MBZ71_002024"
     CDS             complement(9399..9785)
                     /locus_tag="MBZ71_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4429948.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2010 g         2892 t
ORIGIN      
        1 attttgtcgc tgctcctttt aaatttattt tatcattcgg aagaacttgt ggtcgttccg
       61 tgaagttctt ggtacgtttg cgttttataa agatcaacag ttgattgtcc gttgtgttca
      121 ctaaagacac tagttgactt gtcaccaaga gatttttcag cttcttttaa tttctctaag
      181 ccattcttgt agtcataatc actggtatca attgggtcta atccactatt cgtatagaac
      241 cgaagaagat caccattatt gatttggtca gaaacagcta actgtttatc aactttttct
      301 ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt cagtaatagc ttcaccagtt
      361 tcgttactat agatcgtacc accgtagtaa gtatattttg gtgtaacaaa atcaccatca
      421 cggaaagaaa caagctgttc atgatctttc gagaatagat cttgtcctaa ctgaatataa
      481 ttttttgtat ctacacctag taagtgaagt aatgtaggca aggcatcaac ttgtccgccg
      541 taagtgtgat tgattccgcc ctgtgtttgt ccagggatat ggatcatata agggacacgc
      601 tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg tttttccaac taattcagct
      661 agatttttat ttcttgaatt cgagacaccg taatggtcac cgtaaaggac gatgactgaa
      721 ttatcataca agccgctttc tttcaaataa ttgaagaatt cttgtacggc ggtatctaaa
      781 taattggctg tcgcaaagta accgttgatc gtttcatcat ttgtattggc taacgggaat
      841 cctgcttcgt cattggtgaa ttttgaataa ggataatggt tagatacagc aatgaatttt
      901 gaataaaatg gttgttgcag acgttccaag tattgtacgg attgttggaa aaatggttta
      961 tcatgcaacc cgtattggaa cgaattgtca tcattaacat cataataaga agaatcaaag
     1021 aaataatcat atcctaaatt cttgtatgtc tcgtttcgat tccagaatgt accagcgttc
     1081 ccatggaata ccgcactaat gtaaccttgt gtttgactta aaatatcagg tgcagcttgg
     1141 aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt gattcaagcc aaacaatgaa
     1201 ttttccatca gcgtttccgc atcgcttgtt tttcctgctt ttacttgatg gaaaaagttg
     1261 tcaaaactga acgtgctgtt gctgtggtaa agactattga tgaaaggtgt aacttcatgc
     1321 tggagaccat tctcatctgt caatttataa tcgatcagga attgctggaa gctttctaaa
     1381 tggatataaa tcacattttt tcctctagcg agcccgaaca aatcgtcatt tggtgctgca
     1441 taatgttctt ttacataatt ttctacttct ttcatatcat tgggacttgc ttctgcccgc
     1501 acttggtttg tctggtaagt ttgaacaccg tcataaacca tgaaagcatt caagccaaga
     1561 tatttgacca aataatcacg agagaatgtt cgagtcagca attctggacg atcagcttcc
     1621 gccataaaaa gatttcctga gaaaatcata actgctaatg tagagatcgc aaaagccatt
     1681 cttgctcgga tcgggcgagg gtccaacttg attttttttg caatcagcag aacagtcaag
     1741 ataataaaat cgatccaata gatcacatca tatgggcgaa acagtcgaat ggcactttct
     1801 cccagaccac tcgctacttt cccagcacct aaaatcgtgt tgatcgtaat gaagtccgtg
     1861 aattctcgat aataagtgac attcgaaaaa acaagcaatg tcaaaagaaa ataaatgacc
     1921 atcatcgtga tataagcaac tctagggcgt ctgatataaa gagcaattcc aagtaataac
     1981 aatgtagttg caatagggtt gataaacaag ataaagtatt gaatgccact ttcgattccg
     2041 agatggaaat caactaaata ggcaaaaatc gttttcaacc aaagtaaaac gactagtaaa
     2101 gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt ttatacattt caagataatc
     2161 ttcctttctg cacttcttaa tcacagaagt gtcctgaaac catgttacac agctcactta
     2221 ttctcattta ttttactagt atgacaatat agtgtcaatt gaaaccagaa gtttatgctg
     2281 tttcattaag gttttcttta agcttcatga aaaaatgagc agtaagacaa gtcctatgtt
     2341 ttttgttata cttgggaaga gaaagctaaa gcaggtgaag gaatgaaagt aaaagtaaaa
     2401 aaaagagcaa gcaatcgatt gaaaaaagga tatccactga tccaaaaaga agaccttgct
     2461 catccccaaa aagaaaccac gcaatgggtg gaattagttg attcgcaaaa ccaatttatc
     2521 gcaaaaggat atctaggaaa gcagaataag ggaatcggtt gggtattgag ccagcagaac
     2581 atcccatttg atcaatcttt ttttgaagaa ttttttgtga agtcaaaaga aaaaagaact
     2641 ttcttttttt cagatgattc tactaccgct tttcgtatat tcaatggaga aggagacggg
     2701 atcggcggtc tgattgttga ttattatgca ggttatgctg ttttttcttg gtataacgaa
     2761 acgttatatg cacatcgtga attattgatt caagcttttc gtgaaatcta cccggaaatc
     2821 ataggaggct atgagaaaat tcgatttgaa tcagtaaatc tgcctgagag ccagttcatt
     2881 tacggaaaag aggcaccgga acccttgatc gtaaaagaaa acggagtaaa atttgccgtt
     2941 tatttaaatg aaggattaat gactgggatc tttttagacc aaaaagaagt acggggatca
     3001 ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca tgtttagtta taccggcgcc
     3061 ttttctgttg ctgccgcaat gggaggggca atcgaaacaa caagtgtcga tctagcaaaa
     3121 cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg gtttggatgt cgatcagcaa
     3181 aaaatcattg ttatggacgt attcgagtat ttcaaatacg ccaaaagaaa acaattttta
     3241 tatgacatga ttgtattgga tccaccaagt tttgccagaa ataagaaaaa agtctttcgc
     3301 gtagcaaaag attatgggga actggtcaag gatagtctgg atattttagc agaagacggg
     3361 atacttattg cgtctacaaa tgctgcgaat gtctcgatag aacaattcca aacaatgatt
     3421 gaagaggaat tccatgccaa aaatgtttct tttgaacaac ttgctttata tcgtctgcca
     3481 aaagatttcc agacgacgga taccttttta gaaggcaatt atctgaaagt gtttgtttat
     3541 caaattaaaa ataaccatta gaaggagcga tgcagaatgg ttcgggtaaa gggattgaat
     3601 ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg gggccgtccg agaggaagta
     3661 ttacgtgaag cagttcttgc acgtgaagca ggggccgatt tagtcgaatg gcgagtcgat
     3721 cattatcgtg aagttttttc ttttgatgaa gtagcagaaa cattggttct tatccatgag
     3781 atgcttgatg gactgccgtt attatttacg tttcgtacct tagctgaagg cggagaacaa
     3841 gacatatcga tccgtcagta tcgcgacctt tatgaacatg tgatacacac aggattagta
     3901 gatatggtcg atatcgagtt gttcaaagta gaaagtctag gaaaaagact gatagaggaa
     3961 atcaagtctt taaacattcc tttaattatc agtagtcatg attttaaaga aacaccagca
     4021 gaccctgtct tgctttaccg attgaatgtt atggaacatt ttggagccga tatcggaaaa
     4081 ctggcagtaa ctcccaataa tgaacgagat gttttgcggt tgatggagtt gactagacgg
     4141 gcaaatgctt ttgtctccat gcctattatt acgatgtcca tgggaaatct tggtaaaatt
     4201 tctcgtctgg ttggcggaac gacgggttcc gttatgacat ttggttcctt aagcagcgat
     4261 tctacttctg ctccaggaca attaccggtc aaagcattga agcaaattat tcaattatta
     4321 aatgaaccgt tgtagaacaa gtttgaggtg atattttttg gcaaagaaaa aaatagtaaa
     4381 aacgaatgca atccgtatgg tcgaacaaaa aaagattccc tatacagagc acgaatatga
     4441 gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa cagcttccag aaagccaatc
     4501 tcgtatattt aaaacactgg ttgctgtggg caatgtgacg gggcctcttg ttgctgtgat
     4561 accgggagaa gccgaattga atctaaaaaa attagcaaaa gtttcaggga ataaaaaagt
     4621 agaaatgctc catttaaaag atttggaagc aacgaccgga tacatacgtg gtggctgctc
     4681 gcctataggt atgaaaaaac tatttcctac ttatttagat caaattgcag aaagctatga
     4741 acaaatcatt gtctcggctg gcagaagagg gctacagatg gagttagcac ctcaagatat
     4801 ctgtgcatta acgagcggac aatttgcaga tataaaacaa taaatcatac gtataaaaga
     4861 aagaaggagg ccgggatgtt gtttgtccag tctccttctt tcttttttat acattatgct
     4921 ctttgcccgg cattcattcc tgctacatgc cctgtagcga aagccgctgt gatattaaag
     4981 ccgccagtat aaccattgac atccagtaat tctcccgcaa aatacagacc atcaatgatt
     5041 ttactctcca atgttttagg ataaatttcc tttagagaaa cccctccgcc agttacaaat
     5101 gatttctcaa taggaaaagt tttgttgatt gatagcttaa actctttgca tagttgaaca
     5161 aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca gatgatccat ttcaagccgc
     5221 tcaagaaaga aaactaacaa tcgctcaggc aatataccat gccaagcatt gaccaagttc
     5281 tttttggttg ctttactttt ttctgtcaat atatgaataa gctcttgttt ggtttgtgta
     5341 gggaaacaat ccagagaaag tgtcactggt tctcccgttt tctctaattc tttattgaca
     5401 aaacttgaac aacgcagtgc agccggacca gaaataccga aatgggtgaa aagtaaatcc
     5461 atcgtatgtt cagtcaatac acgacctttt ttatttaaaa cgcgtaaagt gatgtcttgt
     5521 aaagaaagcc cttgaagtgt tttctcctga atatacggtt catccgatat caagggggac
     5581 tctgtcgcat aaagaggcgt caccgtgtga cccactcgct tcacgatctt gtacccatct
     5641 ccagtagaac cagtagatgg ataagtccgt ccaccagttg ttaagataat ggcaggagca
     5701 tggaactctt cgaattccgt gcgtataccg taaattttat gttctttgtg gatcaatttc
     5761 tctactcgag tagagaatag aagcgtcaca tctagttctt tcagacgatt gagcaatgct
     5821 tcgataatcg tttttgattt atttgttaca ggaaacatcc gtccatgatc ctcttctttt
     5881 aaatgtacgc cttgtgattc aaagaagttc atgatatcga aattattcca ttgcgcaaac
     5941 gtactatata aaaactttcc atttccagga atatgtgcaa tcaaatcatc aacaggacga
     6001 ttgttcgtaa cattgcaccg accaccacct gtcatcagta gtttttttcc tgcttttttg
     6061 tttttttcga tcaagagtac gcgtgcgcct tgttcagctg cactgatcgc tgccatcatc
     6121 ccgcttgttc cagcgccgac tacgatcaca tcatacagtt tttccattcc ctcacctcaa
     6181 ggaaagtgta gcatatctga agaaagaaaa cagaaaaaaa tgttttgagc caaaagagaa
     6241 aatgagctaa aatgagggaa gataagcaaa ggagcgtgga acgatgacaa acgcaacaga
     6301 atatgttcaa gcaattcaag aaaagctaca tcaaaaggat caagggcaag aagaattttt
     6361 acaagcaatc gatgaattca tgcccacggt tatgtctttt ttggacactc atcccgaata
     6421 tatcgaaaag aatatcttag aacttttaac ggaaccagaa agagttattc aatttcgagt
     6481 gccttggcaa gatgatgaag gaaactggcg agtaaataga gggtaccgta tccaatacaa
     6541 ctcagctatc ggcccttata aaggtggatt gcgcttccat cctagtgtca atctaagtat
     6601 cttgaaattt ttggcttttg aacagatttt caagaatagt ttaaccggtc tgccaatcgg
     6661 tggaggaaaa ggcggaagtg acttcgatcc taaaggaaaa tctgataatg agatcatgcg
     6721 tttttgtcaa agtttcatgc tggaattgtc aaaacatatc ggcccatcga ttgatgttcc
     6781 tgcgggagat atcggtgtag gagctagaga aatcggctat ttgttcggcg aatataaacg
     6841 attgaaaaaa tatgatactg gtgtgttgac aggtaaacct ttggattttt ggggaagtaa
     6901 gatacgtacg gaagctactg gttatggatt agtgtattat gtcaaacatc tattgaacga
     6961 agaaaaagat tcatttaatc aaaaaacagt ttttgtttcc ggaagcggga atgtagccat
     7021 ttatgcaatc gaaaaagtgc aagagctagg cgggaaagtt gttacatgtt cagattctag
     7081 cgggtacatc tatgatccag aaggaataga tgtgaaacta ttgaaagaag tcaaagaaat
     7141 caatagacaa cgtttgacgg aatacgcaaa ccaacgtcct tctgctattt atcatgctgg
     7201 agaatctgtt tggacattga aagaaaaagc ggatatcgct ttgccatgtg ctactcaaaa
     7261 tgaaatagac gaaagcttgg ccgaaatctt agtagaaaat gggatcaaaa tcgtagcaga
     7321 aggcgctaat atgccatgta cagtaaaagc tgtttctgtt cttcatgaag caggcgtgtg
     7381 gtattgtcca ggaaaagctg ctaatgctgg aggagtagca gtatctgctt tagaaatgag
     7441 tcagaacgtc cagcgattga cttgggaaag agagcaagtg gatgagcagc tggatcaaat
     7501 catggaaacg atttaccata cgtgtcgcca aacagcagcc gagtacggcg acgaaaaaaa
     7561 tctgcttctt ggagcaaatg ttgctggatt tgaaaaagtt gccaaagcaa tggcagcaga
     7621 aggtttagtt tgaaaaatga aactttcttg gaaatattgt cagcattttg tttatttgct
     7681 tgaaaaataa taaaaaaaaa cgtaaagtat tacctgtaga gtaaacctat aaggaggaga
     7741 acaaatggca cacattcatt ttgactattc aaaagtagca ccgtttgtca acgaacaaga
     7801 gttagggtac atgcaaagcc aagtaacagc agcccacaac gaattacggg aaggtactgg
     7861 cgcaggaagc gacttccgcg gctggatcga tttaccaacg aactatgaca aagaagaatt
     7921 tgcacgtatc aaagaagcag ctaaaaaaat ccaatctgat tctgaagtat tagttgttat
     7981 cggtatcggt ggttcttatt taggcgctcg tgcagcgatc gacttcttga atcacacatt
     8041 cttcaattta ttagatagca acacaagaaa agcaccacaa atcttctttg ccggaaattc
     8101 aatcagctca acatatattg ctgacttgat cgaagtaatc ggtgatcgcg acttttcagt
     8161 aaacgttatt tcaaaatctg gaacaactac tgaaccagcg attgcgttcc gtgtattcaa
     8221 agagcttctt gttaagaaat atggacaaga agaagcaaac aaacgtatct acgctacaac
     8281 agataaagca aaaggtgcag ttaaagtaga agcagatgca gaaggttggg aaactttcgt
     8341 gattcctgat gacatcggcg gacgtttctc tgtattaact gctgttggtt tgttaccaat
     8401 cgctgttagt ggtgcagata tcgatgcatt gatgcaaggt gccgctgatg caagcaaagc
     8461 atactcaagc gacaaactag aagaaaacga agcttaccaa tacgcagcaa tgcgcaacat
     8521 tctttaccgt aaaggcaaag taactgagtt gttgatcaac tatgaaccag gaatgcaata
     8581 cttctcagaa tggtggaaac aattgtatgg tgaatctgaa ggaaaagacc aaaaaggcat
     8641 ctatccttca agcgcaaact tctcaactga tttgcactct ttaggacaat tcatccaaga
     8701 aggacgtcgc aatatttttg aaacagttgt gaaggttgaa aaacctcgta aatcaatcac
     8761 aattcctgaa caagcagaag atttagacgg tctaggctac ttgcaaggaa aagaagtgga
     8821 cttcgtaaat acaaaagcat ttgaaggaac attgcttgct catacagacg gtgacgtgcc
     8881 taacttgcta gtcaaaattc cagaaatgga tgcttataca ttgggttata caatgtactt
     8941 ctttgaaatt gctgtaggta tttcaggtta cttgaatgga atcaacccat tcgaccaacc
     9001 aggtgtagaa gcttacaaga aaaacatgtt cgcacttcta ggcaaaccag gctttgaaga
     9061 tcttgctaaa gaattgaacg aacgcctgta agataagggt ttttcgctag atttaaataa
     9121 tcagtgatag aaacatgttc gctttaaaat gaaaactggt acccatttag gtactcaaac
     9181 atactgcatc gtttattcag aataaggggc tgtgacaaga cgtttgtcac agtcccttgt
     9241 ttgtatagaa atcgattgat tgccttgctc tttagtttgt tttaggactt taagaacagt
     9301 gaggctgact tcgaaggtta attctgtttt tttgttttct agaatcagta agcaaaaaag
     9361 cactcacttc gagtgctttt ttgcgatata ttcttagctt aagataggcc taatttttct
     9421 tttacagatg agggtacctt atcgctggta attacttttt tctctatgat ccgcttttcg
     9481 cttgctttca actgcaagat cgttccttcg gggtaagcag aaccattggc agagtcacca
     9541 gaagtaatga catcgaaatc taactgtctt ttttcaccgt tttcagcata agcatctacg
     9601 ttgtatttaa aacctcttcc aattacttcc ccattatctg ctttgatatc agtttctgca
     9661 ggcattggag ctttgactac cgcataataa tctttaccta cataagtaga ctgatagtag
     9721 tcccaacctt tccatcctat aaaagcaatc aatatgataa cgatcaaccc taaaattttt
     9781 ttcatgaaag atctcctttt ttatttttat ta
//
LOCUS       DAGCWU010000109         9754 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.109, whole genome shotgun sequence.
ACCESSION   DAGCWU010000109 DAGCWU010000000
VERSION     DAGCWU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.109"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..173)
                     /locus_tag="MBZ71_002025"
     CDS             complement(<1..173)
                     /locus_tag="MBZ71_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429949.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQT"
     gene            complement(189..803)
                     /locus_tag="MBZ71_002026"
     CDS             complement(189..803)
                     /locus_tag="MBZ71_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBT4429950.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(950..1633)
                     /locus_tag="MBZ71_002027"
     CDS             complement(950..1633)
                     /locus_tag="MBZ71_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBT4429951.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1742..2200)
                     /locus_tag="MBZ71_002028"
     CDS             complement(1742..2200)
                     /locus_tag="MBZ71_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBT4429952.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2284..4047)
                     /locus_tag="MBZ71_002029"
     CDS             complement(2284..4047)
                     /locus_tag="MBZ71_002029"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBT4429953.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4268..5728)
                     /locus_tag="MBZ71_002030"
     CDS             complement(4268..5728)
                     /locus_tag="MBZ71_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429954.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5936..6862)
                     /gene="glsA"
                     /locus_tag="MBZ71_002031"
     CDS             complement(5936..6862)
                     /gene="glsA"
                     /locus_tag="MBZ71_002031"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBT4429955.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6885..8312)
                     /locus_tag="MBZ71_002032"
     CDS             complement(6885..8312)
                     /locus_tag="MBZ71_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4429956.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8343..9557)
                     /locus_tag="MBZ71_002033"
     CDS             complement(8343..9557)
                     /locus_tag="MBZ71_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBT4429957.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2890 a         2033 c         1621 g         3210 t
ORIGIN      
        1 gtttgatctg gcatttctat ttccttacca tctaaagtaa cagtagtaac gacaaaatgg
       61 tcttgcaatg gcggaacttc taagtactgc ccttgcagaa aatcattcac tgtttcatat
      121 tcttttttca tttcttttga atctgtcgat ggtttatagc ttatttttaa cataactagt
      181 gctccttttt attcgggacg tgcaatcgtg aaaatttctc ctattgctgc ataatcattt
      241 cctgccaaac ggcttacagc attcaatttt cttggatcga tccttcctgt cttatcatca
      301 tagactgttt catttaaatg atatttctgt acttctagca aaaataaatc agctgttact
      361 ttatttttat tccgtatctc gacatgttca tataaggatg tttcaaaacg aacttttgct
      421 tctgtcaatg ctggtactga aatgattact gaatcagtta aagtcaagtc agtcaggtac
      481 agttcgcttt cctcattagg cagattggct gctgttttat ttacttcttc tacgttgtcc
      541 tcatcaagga tatggataac agcttctctt gtctcaagaa tattctttgc tgtgtctttt
      601 actgcatctt gttttctctg gatcgccaag gataagatgg gtcgattcga actgacgata
      661 ttgaagtaac tgaagggtgc gatattcact atatcttttg ctgatttagt cgttactact
      721 gctactggtc tagggatgac tgaaccaatc agcaacttgt aattttcacg ttctgtcaat
      781 tctgaaggac tccgttcaaa catggtttcg ccttctttct ataaaattcg ttaaaaagca
      841 gatcttgaag acaaaatgaa aagtaatgct tttttcatct tcttagaatc tgctttaaac
      901 tatctatcaa tttttctttt ttgtcaagaa tctgtgccgt gcgtgttttt tagttatttg
      961 ctttacttgc ggcttcgaca tatcttttag tgatcaattg cggacgggtg ataatagatc
     1021 caactacgac tgaaaaagca cctaattcat atgctttctc tacttgttca ggagagtcag
     1081 tatgtccttc cataattact gggatatcga cttgttctat gatttgattc aataaatcaa
     1141 aatcaggtgt ctgcgtcttt cgtgaatatt ctgtatagcc tgctaaagtc gtagacaaaa
     1201 gatcaaaacc atcttcttgc gcttgaagcg cttcctcaaa agtcgaaata tctgccatag
     1261 ctaaccgacc gtgttcgtga atcatttgga caagttcagc agttgttttt tctgtcggac
     1321 gtttacgaga agttgcatcc agtgcaatga tttctgcttg cgtctccaaa agttcttcca
     1381 cttctttttc tgtcgcagta ataaacactt ctgacccatc gtaatctctt ttgataatcc
     1441 ctattaccgg taaatcaacg attcgcttga tttcattgat atcttctgtc gtatttgctc
     1501 gaataccaac tgctccgcct tcttttgcag ccaaagccat tctccccata ataaaagaac
     1561 tgtgtagcgg ctcgttttcc aaagcttgac aagaaacgac gactccgcct ttgatttgtt
     1621 ccagtgtatc cacaatatct cctccagtaa tgttatataa aaatacagct tcatataaaa
     1681 atgtaaccct tttctattca ggagacaata atgagctatt tctttcagca gacctttctt
     1741 actaaaagaa aattacttca aattcttcgt atacgaccag cttctcttct gtaaattcta
     1801 tgttcaacgc ttctttataa gcatgtttaa aaaactcagc atggactttc cgccaataag
     1861 ctaatgtccg atccccttct ccttctttcc aagcatgctc tttcgtgact tcactaaaag
     1921 gcaagatggt cacatttata tttttgatga tacaaactgg ttcatttttt ccattcagga
     1981 ccacgtctaa ttgaccaact tttggtaaag gctcattctc ttgtttatac agatcgtaac
     2041 cacttgatgt accagtcttt atctttttta caactagttc gctcaactcg tctgccattt
     2101 tttctgtatc cccaaatgaa aagaaaccta atttttctga ttgataacta ggatattttt
     2161 cttggaactt tttgacatat tcttctactt gctgatacat aacatccacc cctttgcttt
     2221 ttataatgaa aaaaccatcc agatactagt ataaacaatt atctggatgg tgagcataat
     2281 gaattattga atattcaaga cacgcagact atcttcaacg atttgttttg gtgttaaatg
     2341 ataacgttca taaagctgcc ctacaggaac gctatccgtg aattctttct ctgcaccaaa
     2401 atttaatacg ttcattgaag aatcaccata ataacgactg atattttcgc caaatcctcc
     2461 tgacaaacta ccatcttcta atgtgatgac taattcgtgt gtttctttta atttatctaa
     2521 tgtttctacg tcattttctg tgatcgttcc tggattgatc aacgttgctt cgattccttt
     2581 ttttctaaaa gcttttactg tttcttttcc taattcaaga aaaccgccta aagctaaaat
     2641 tgctactttt cctccattat ttactgtttg ataggctgga atactataat catttgcgat
     2701 agctgtacca tgttctacac catgtgtagg aattcggatt gctacaggct gcgctgtttg
     2761 ttcgaacgcc caatctagca tagagatcat atcctctttt gttgctggag caagatattt
     2821 gatgttcggg atacttgata agtaaggaat gtcaaatgtt ccttggtgtg ttcggtcact
     2881 agctgagatc gttccgccac cgactaaaat aatcactgga ttctcgttga tcgctaaatc
     2941 atgtgataat tgatcatatg cccgttgcaa gaaagtactt gattggaaga cgactggacg
     3001 tactccctga gaagccaaag cagaagcgaa agtgatagag tgctgttcag cgatccccac
     3061 gtcaaaataa tgatctggat gtttttcttg gaatgttttc agtccaaagg cgcctggtat
     3121 cgctgcattg actgcggcaa taggagctgt acttgtttgc attttcttat ccaaataatc
     3181 catgatgatc atattaaatg tttctccaga agttacttgc tttgcctccc cggtggacag
     3241 atcaaatggt acatgccaat gatatttcat tttattatcg attgccggct gataaccgcg
     3301 acctttttcc gtatggacgt gaagtacaat cggatgatca atatctttta cttcttcaaa
     3361 taaacggatc accgtttcca gatcgtttcc attatccacg taacgataat caaatcccat
     3421 tgctttaaat aggttattgc tgcttttacc gttggtttga cgaagctcag ctaagttttt
     3481 atataaactg ccatgattct catcaataga catctcatta tcgtttacta atacgattag
     3541 attggattgt aaaacaccac cattgttcaa tccttcaaaa gctaagccac cacttaggga
     3601 gccgtcaccg ataaatgcta ccacatttcc tgattctttc ttcagatctc tcgctttagc
     3661 taaacctgtt gctaagctga tagatgtaga tgtgtgcccc actgtaaaat agtcatgttt
     3721 actttcatgt tggttggaat atccgctcac ttcacggtaa tgtccttttt caaaaccatt
     3781 ttttcgacca gttaaaagct tatgaccata agactgatgg gaaacatccc aaacgatttt
     3841 atcctttggt gaatcaaata cataatgaaa cgcaatcgtt aattctacta cacctagatt
     3901 cggaccaacg tgcccaccta catcgctgtc cttttgcaag attttttggc gcatctcttc
     3961 tgccaactgt ttcatttcct caacagataa ttttttgaca tctgcggggc tattgattgc
     4021 atccaataca tgtaatactg tactcataat acattctctc ctccgtacta tcttctttta
     4081 atgaaaatag atttgttaca ctttatctat tctactactt agagttgact ctaagtcaat
     4141 agcattttaa aaaaatttca gagaaagctt gttatataag tagatcttcg cacgaatcag
     4201 gactcaaata aaaaccttct acagacaatt aaaaagcttt gtagaaggtc tcctaattaa
     4261 ggtctcatca agaaatatgt ttttttaata ggtgaagttc ctgagcaata tgatacaaat
     4321 ggatgtttgc accttttaat gagattggat agatctttgt cttttcagtt tctaatccat
     4381 tttgatagct agcagcatcg cacaaaacca ctcttttttt cgcacataga cattcatcag
     4441 gcaattcttc taatgaacga aataaaatag gatttatttc agcgatgcct tcttccgcat
     4501 acaaatgaaa ttgggttcga taaaaaaatt gtttgactga actttctgaa acaacaaaaa
     4561 tttctatttc ctgtctcccc gcttgttcac tgttgatcaa agaaagactt ttgacgaatt
     4621 ttcgaatgaa attttcattc catcttagtt ggatttgatt tttctttttc cactcttcca
     4681 atatttctac aatttttccg tacagaagtt gttctttttc tgatagaaga tagatccttt
     4741 ccggtaaaaa tacttgtcca tctgcccaca acgtacggac aaattgtaaa aaggattgct
     4801 tgaaaataaa attttctgca tcgtcgattt cagcgatact aataatctgt aaaatcaatt
     4861 cattcagtga taggtcattg agaacaaaat ttttataaac aacttcgaaa tcttttctta
     4921 acagctccat gttctcattg gtataatttc tactgttgaa caaagaaaaa atataataga
     4981 catcttcttc ttgtaagtct agaattcctt tttgctcttc caatccttga cagactaatt
     5041 gaaataacgg cttcgtttct gcgcttttct tatcttcttc tttcagtcca aggggaaatg
     5101 cctttcttcc gattgcgatc tctattcctc gtatgataaa ggacttttca tcttccgaat
     5161 atcttctttt aaaaaaatga gtctctacat aatcaatcaa ctcgttacaa gcttcttcta
     5221 catacggatt tttattttca tagccttccc aattgacata acgtactaaa gctagcaaga
     5281 tgttccgcga gtcaaattct gggataatga tttctttatt tttcaaataa tcattttctt
     5341 taaaaaaatc caatacgatt tttttgattg tatatacttt gctcaaagaa ataaattcaa
     5401 gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa aactttaaaa
     5461 aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca tgtttgaata
     5521 cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct agacactcgg
     5581 ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa atagctgttt
     5641 gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact tttctaaata
     5701 tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt ttcttatgat
     5761 tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga aaagcccttt
     5821 caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa tttcagcctc
     5881 attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag aatggttatt
     5941 caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata ccagctgcgc
     6001 tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga tttggcacag
     6061 ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg acggaaaact
     6121 ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt gtagcacttt
     6181 cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc agcacagcag
     6241 ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa taagcatcga
     6301 gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa gcaagagagc
     6361 gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat gtgatattag
     6421 gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca tctgcatctt
     6481 taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga ttcattggat
     6541 gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg gttggttccg
     6601 tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat aacaatacgg
     6661 gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc tcaatcagtt
     6721 gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact tcagctaatg
     6781 ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt ccaatcaatt
     6841 gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt ttctgcatgc
     6901 tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat aagataagaa
     6961 acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc aggtggaaca
     7021 aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac gattactttt
     7081 acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt aaaaaccagc
     7141 ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac agtcaatgac
     7201 atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac ggctgcccag
     7261 attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg tgtgttttct
     7321 tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc ttttgaagga
     7381 gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat cgcaagaatt
     7441 ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa cgattgaatc
     7501 acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt caatccgcca
     7561 aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg ataatccttt
     7621 ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat atatgctaaa
     7681 ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat caatgttttc
     7741 atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc taagccaaac
     7801 aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat caatgaagtg
     7861 tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa taggattgcc
     7921 aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga taatgcacca
     7981 gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg gaaaaagatt
     8041 gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac accgcctttt
     8101 tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat aaaccatagc
     8161 aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa tgtgggatat
     8221 tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa cgttaatttt
     8281 tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta tttgaatttg
     8341 tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg ttcttctttt
     8401 gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt cagcacatag
     8461 gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta ccagctgctt
     8521 ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt acctgtgaat
     8581 ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt ctttttacga
     8641 aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat ggcacttttt
     8701 gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga tcctgttagt
     8761 acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca agtaatcatc
     8821 gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc atctgctcga
     8881 agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt accgatagca
     8941 gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct ttgaccaagt
     9001 gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc tccaccagcg
     9061 aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg gcaaacaggg
     9121 aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa attcaatctc
     9181 cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag ttggtaaaga
     9241 aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct tgtaacatgt
     9301 ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg tccaaaggcc
     9361 aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag aaaagattga
     9421 gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa agctagacca
     9481 ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc tcctgtattg
     9541 ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata aaagaaggtg
     9601 tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa gacagacaat
     9661 taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa gacaaaaaat
     9721 aaacggtctt ctttttatag aaaaccgttt attt
//
LOCUS       DAGCWU010000110         9671 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.110, whole genome shotgun sequence.
ACCESSION   DAGCWU010000110 DAGCWU010000000
VERSION     DAGCWU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.110"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(19..339)
                     /locus_tag="MBZ71_002034"
     CDS             complement(19..339)
                     /locus_tag="MBZ71_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBT4429958.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(487..2721)
                     /locus_tag="MBZ71_002035"
     CDS             complement(487..2721)
                     /locus_tag="MBZ71_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4429959.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3200..3466
                     /locus_tag="MBZ71_002036"
     CDS             3200..3466
                     /locus_tag="MBZ71_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBT4429960.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3466..5193
                     /gene="ptsP"
                     /locus_tag="MBZ71_002037"
     CDS             3466..5193
                     /gene="ptsP"
                     /locus_tag="MBZ71_002037"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBT4429961.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5357..6403
                     /locus_tag="MBZ71_002038"
     CDS             5357..6403
                     /locus_tag="MBZ71_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBT4429962.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6420..7643
                     /locus_tag="MBZ71_002039"
     CDS             6420..7643
                     /locus_tag="MBZ71_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBT4429963.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7732..8619
                     /locus_tag="MBZ71_002040"
     CDS             7732..8619
                     /locus_tag="MBZ71_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBT4429964.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8705..8935
                     /locus_tag="MBZ71_002041"
     CDS             8705..8935
                     /locus_tag="MBZ71_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBT4429965.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(8992..>9671)
                     /locus_tag="MBZ71_002042"
     CDS             complement(8992..>9671)
                     /locus_tag="MBZ71_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HBT4429966.1"
                     /translation="NYNYVHITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQ
                     MPWLSEISLLALRAQLPDLQEMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLN
                     FEKAGMLQKDIELVTYFIRRIQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAE
                     TMIASADVSPDFYYEKKEAHLSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKK
                     EAEKQLN"
BASE COUNT         3083 a         1713 c         1970 g         2905 t
ORIGIN      
        1 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
       61 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
      121 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
      181 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
      241 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
      301 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
      361 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
      421 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
      481 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
      541 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
      601 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
      661 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
      721 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
      781 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
      841 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
      901 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
      961 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     1021 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     1081 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     1141 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     1201 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     1261 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     1321 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     1381 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     1441 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     1501 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     1561 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     1621 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     1681 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     1741 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     1801 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     1861 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     1921 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     1981 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     2041 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     2101 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     2161 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     2221 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     2281 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     2341 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     2401 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     2461 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     2521 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     2581 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     2641 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     2701 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     2761 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     2821 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     2881 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     2941 atgataaacg tcaggagaag agaaagcgac aagaaaaaga aaataaacac gtttgttata
     3001 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     3061 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     3121 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     3181 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     3241 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     3301 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     3361 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     3421 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     3481 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     3541 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     3601 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     3661 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     3721 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     3781 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     3841 caagagcgtg ctgcagatat tcgtgatgtt gctaaacgta ttttagcgca cttattaggc
     3901 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     3961 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     4021 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     4081 ggtacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     4141 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     4201 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     4261 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     4321 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
     4381 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
     4441 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
     4501 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
     4561 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
     4621 gttcatggta acttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
     4681 gcaaaagcga tctttgaaga agaaaaacaa aaattgatca gtgaaggcaa ggaagtatct
     4741 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
     4801 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
     4861 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
     4921 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
     4981 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
     5041 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
     5101 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatac gatggaagaa
     5161 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
     5221 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
     5281 tagataaaaa tagggcttaa gtttgagtga cagtttgtag gctatgttat actatataag
     5341 tgaaggggga cgtaaggtga aggtattatt atattttgaa agtgaaaaga tgcttgcaaa
     5401 atctggaatc gggcgtgcgc ttgatcatca aaaacgtgct ctgacggaag tcggtatctc
     5461 ttatacacta gatgaaaaag aagattatga catcctgcac atcaatacat acggaatcaa
     5521 tagtcataat atggtcaata aagccagaag agaaggaaaa aaagttgtct atcatgcgca
     5581 ttctacagag gaagatttcc gaaattcctt tatcggatcg aaccagcttt ctcctatcgt
     5641 aaaaaaatat ctggtaggat tgtaccaaaa agcggactat ctgatcacgc caacacctta
     5701 ttcaaaacag ttgttggaaa gctatagtat ccgtgtgccg attcaagcta tttccaatgg
     5761 gatcgactta gagaaatatc gccctgatcc aatgaaagaa caaaagttca gagaatattt
     5821 caaactgtct ccggatcaaa aagtaatcat ttgtgtcggt ttgtttttcg aacgtaaagg
     5881 aatcattgat ttcgtagaaa ttgctaagaa gatgcccgaa tacacattta tttggtttgg
     5941 tcatgttccc atgtactcga ttcctcggaa catccggaaa attgtaaaag aagatcatcc
     6001 tgaaaatgtc ctgtttcctg gttacattcg tggagagatc atagaaggtg cgtattcagg
     6061 tgcagatcta ttctttttcc cttcttatga agagacagaa gggatcgttg ttttagaagc
     6121 tttagcaagc aaacaaaacg tacttgttcg agatattcca gtgtataatg gctggttaga
     6181 agatagcaag aactgctata tggggaagac aaacgaagaa tttatccgac tgatccaaca
     6241 gattaccaat caagaactac cgagtacgac acaagccggt tatcaaacag caaaagagcg
     6301 aagtatcaat aaaatcggtg aagaacttaa aaatgtatac gaaagtgtac tgaatgaaaa
     6361 agtctctagc aaattaaaaa aagtaaatca ggtcaaggac tagtttggag gcgaaaaaca
     6421 tgaaaatcgg cttttttacc gatacctact ttccgcaagt cagcggagtt gctacatcga
     6481 tcaaaacatt aaaacaagaa ttagaaagac atgggcatga agtctatatt tttacaacga
     6541 ccgatcctaa tgcggataag ttggaaaaag atgtgattcg aatgccaagt gtaccattta
     6601 tttctttcaa ggatcgaaga gttgttgtta gaggcatgtg gtatgcttat ttggttgcca
     6661 aagaactaga actagatctt attcataccc atacagaatt cggagcaggg atacttggga
     6721 agatggtagc taagaagttg aaaatacctc tgatccatac ttaccatacg atgtatgaag
     6781 actaccttca ttatatcggt aaaggaaaag tgatcaggca atcccatgta aaatacttgt
     6841 ctagattatt tgcaaatcat acgacaggtg tagtttgtcc aagtgaacgt gttattgata
     6901 cattacgtag ctatggtgta atagcaccgc ttcgcgtgat tcctactggt atcgacattg
     6961 aaaaattcaa acgttctgat atcactcagc aggatatcag caaattaagg gagtcactag
     7021 gtcttcagga aaaccatttg atgattcttt ctttaagcag gatttcttat gaaaaaaata
     7081 tccaagcctt gattaatggg tttcctcagg tcatctccgc ttatcctaac gccagattag
     7141 ttatcgtagg aaaaggacct tatgtagaag agttagagga attgatctca gaactgcagt
     7201 tagaagaatt tgtgcagttc acaggagaag tggacaacaa tgatgtagca ctttattata
     7261 aagcggcaga ttattttgtc agtgcttcta cttcagaaac acaagggctt acttatacag
     7321 aagcgatggc agccggaaca ccttgtgtcg tggaaggaaa tgcttatttg aaccgattgt
     7381 tcgatcatcc ttcattaggg aaaacttttg aaaccgacga agactttgca ccagcactta
     7441 ttgattatat ttccagtgaa gttcctaaag atccaaaagt tttagaagct aaactttatg
     7501 atatttcatc tacacatttc ggcgaagcta tgatcgaatt ttatcaagac atggttgttt
     7561 atcatgaaga agagatgcga agaaaagaag aagaagactc gattgaacga atcaaaataa
     7621 aattaaattc atttaaaaga taaaattcaa acatttttta tgaaaaaatc cttgtttcta
     7681 gcgtatacta gcatagaagc aaggattttt gatttttagg aggtcgaaaa aatgaaactt
     7741 ggttttatcg gaacaggcgt catgggaagt gctgtcgcac gccatctact agaagcagga
     7801 cacgaagtag ctgtctataa tcggacaaaa gcaaaagctg atcctttagt aacagaagga
     7861 gctatctggg cagatacacc aaaagcggta gcagaacaaa gtaatattct ctttacaatg
     7921 gtaggttacc ccaaagatgt agaagaaatc tactatggac agtcggggat tttttctgca
     7981 gatatctcag gacatatttt agttgacttg actacaagta caccaagcct ggcagaaaaa
     8041 attgcaaaaa cagcaaaaga aaaaggcgca gatgcgttag atgcaccggt atccggtggt
     8101 gacttaggag caaagaatgg tacactgacg atcatggtcg gtggagaaga agccgtttat
     8161 gatcaagtac tgcctttatt caaagaattt ggtacgacat ttacattgca tggttctgct
     8221 gggaaaggtc agcatacaaa aatggctaat cagatcatga ttgcaggtac gatgacagga
     8281 atgacggaaa tgcttgtcta tgcacaaaaa aacggacttg atttaaagaa agtgattgag
     8341 acgctttcag gtggaagtgc agctaactgg tctttaagca attattctcc tcgtatttta
     8401 aaagaagatt acacacctgg tttttttgtc aaacatttta tcaaagattt aaagattgca
     8461 ttagaagaag cagaaaaaat ggaccttgta ttgccagcaa ctacccaagc attgaaacta
     8521 tatgaagagt tggcagataa aggttttgag aatgacggaa cccaagcttt gatcaaactt
     8581 tggtggcctg aaggaaaaat accagaaaaa aaatcataaa aaacgagttt ttttagagaa
     8641 aatcaatgca agtaggctat gcttttgata caatataaca gaaataagaa aaggaggacc
     8701 tccgatgaca cattcacaat tagtcgcaat cataaaacga ctggaggcta tggtcgagtc
     8761 agcagataat gaactgcaag ttcgccgttt tgaaaaagaa ggcgtagaga agtgtacagt
     8821 aacttatgat aaagcgaccg atacatttga attaactgaa acagatactc atcagcaata
     8881 cgaatttgat aatatcgata tcgtagcgat ggaaatctat gacttaatcc aataaatttt
     8941 agaataattg acatttgaag ttgaagatgc ctaaaagaag ccgagaagca actaatttag
     9001 ttgcttttca gcttcttttt tgtttaacag ttcaatttca gactgttctt tttctaattt
     9061 tttgcggtaa ctgatcaaga tttgtacagg atcgatttct tcttttgaca attgacgttt
     9121 caaagataaa tgagcttctt tcttttcata atagaagtca ggagaaacgt cagcagaggc
     9181 aatcattgtt tctgcaagtt gaccataaca aacgaggtag tgctttttct gactctcatc
     9241 tgctgctaat ggcatgctga aggtgagcga tggtgttctt aaaaaatgct tttgttcctg
     9301 gatccgtcta ataaaataag taactaattc gatatctttt tgaagcatac cggctttttc
     9361 gaaattcaac tgatagatga aatgttgttg tctttttttc aagtaagtta gtagcttttt
     9421 attttggcct tgaaacagcc cttggagttc cttttttttc tgttcaaaag tcatttcctg
     9481 taggtcagga agctgggctc gcaaagctaa caaactaatt tcagacaacc aaggcatttg
     9541 ataagtttct tccattatac gcaaaataga cggcattttt ttatagagac gaaaaggccc
     9601 gtatgttcga gccgtaggag tatctgttaa tacaaatccc ttattggtaa tatgtacata
     9661 attataatta c
//
LOCUS       DAGCWU010000111         9638 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.111, whole genome shotgun sequence.
ACCESSION   DAGCWU010000111 DAGCWU010000000
VERSION     DAGCWU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.111"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            57..479
                     /locus_tag="MBZ71_002043"
     CDS             57..479
                     /locus_tag="MBZ71_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4429967.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(645..1838)
                     /locus_tag="MBZ71_002044"
     CDS             complement(645..1838)
                     /locus_tag="MBZ71_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4429968.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2062..2439
                     /locus_tag="MBZ71_002045"
     CDS             2062..2439
                     /locus_tag="MBZ71_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4429969.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2427..2765
                     /locus_tag="MBZ71_002046"
     CDS             2427..2765
                     /locus_tag="MBZ71_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4429970.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            2899..3342
                     /locus_tag="MBZ71_002047"
     CDS             2899..3342
                     /locus_tag="MBZ71_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBT4429971.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3604..4419
                     /locus_tag="MBZ71_002048"
     CDS             3604..4419
                     /locus_tag="MBZ71_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4429972.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4429..5091
                     /locus_tag="MBZ71_002049"
     CDS             4429..5091
                     /locus_tag="MBZ71_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBT4429973.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            5296..6960
                     /gene="alsS"
                     /locus_tag="MBZ71_002050"
     CDS             5296..6960
                     /gene="alsS"
                     /locus_tag="MBZ71_002050"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBT4429974.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            6973..7683
                     /gene="budA"
                     /locus_tag="MBZ71_002051"
     CDS             6973..7683
                     /gene="budA"
                     /locus_tag="MBZ71_002051"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBT4429975.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            7813..8310
                     /locus_tag="MBZ71_002052"
     CDS             7813..8310
                     /locus_tag="MBZ71_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBT4429976.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            8495..8755
                     /locus_tag="MBZ71_002053"
     CDS             8495..8755
                     /locus_tag="MBZ71_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429977.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(9117..9356)
                     /locus_tag="MBZ71_002054"
     CDS             complement(9117..9356)
                     /locus_tag="MBZ71_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429978.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         3259 a         1583 c         1922 g         2874 t
ORIGIN      
        1 gtatccgttt tttttcttgt atgtagtata gtaaagtaga tacaggagga gaaaatatgc
       61 agactttttt aacaaaagtt tctcaaaaga aaattaaaat gattcattta ctgcttgaaa
      121 cagaacgttg gtgttcaata gaagaactac aaaacgaatt gaaggtatca agtaagtcta
      181 tcttgaatta tctaacagat ttagaagaat tgttccaaaa atatcctgat aaggtcattt
      241 tgaagaatga gaataatcgg cgcttttcta tggaaaagca ggagaacttc ccaatctaca
      301 ttatttatct tcatttctac agaaaatcat ataattatca tttgattgaa ttcatgtatc
      361 aacatcctga gaaaaatttg gaagattatg cagacgctca atatacgagt gtatccacag
      421 tttttcgtta tgctaagtta ttagtaaagt actttgaaat gacggacgtc aaagaatagt
      481 agccgccaga tagaaggaaa ttagcagttg aaaaagctgc cctgcatcaa gtaatacgac
      541 cagccaaacg gaaacagtgt ctcatgtttt tgatttggct ggtcttatta ccgcagatgc
      601 aagcttttgc cacacctctt tcttgtcatt tacagactca actatcaatg ctgcaagctt
      661 tcccttaatg ctgtaacacc agtaagagtt gtcaagacca gcagactgac taggtaaata
      721 ccgctcaaaa acaagattac ggcagttaaa ctataatgat ccatcaagaa accgatgacg
      781 atagaagaaa aaccaccaac agctcgtcca aggttcataa tgaaattatt cgccgtcgcg
      841 cggatttctg ttggatacaa actactaatc actgcgccat atccaccata catgccattg
      901 ataaagtagc cgactatcac agcggctgct accaaactcc actggctatg ggctaacaca
      961 agtagataga cagataagga tgaacaaatt aaaaacaaag aaaaggctag tttaggcccc
     1021 aatttatcca tgattatgcc aaaagtcatc atccccaaag acatgcctaa aatagtactg
     1081 accatccaca atgaagaccc agaaaccgaa agccctaatc gattttgcat aattgaaggc
     1141 agccaattca ttaacccaaa atagccggca atttggacag ttaccatgat actaaggcca
     1201 catgtttgcc aagcaagatg ttttgattca aacagtctgc taagtttggt tttcttttcg
     1261 gcagattgta cagatttgaa ttcctttggt tcagtcaagc cgcgacgaat gaacaagacc
     1321 atcaacaccg gcagtacacc tacgacatac aacatcttcc aaccaaacgt tggaatgatc
     1381 acagcagcta aaatcgcagc caaagccgca ccgacctgcg caccaatacc agcaaccgaa
     1441 gaagctcgtc caatttgttt ttttgaaaaa ctttcggaaa tcaaggacat acacgccccg
     1501 tattctcctc ccgctcctac acctgcgata aagcgaaaga gataaaccaa gtagatgttt
     1561 gaagcaaaat acatcaataa agaagcaatc gaaaacagaa ggaccgtttg agaaaatact
     1621 ttcacacgtc catatttatc agctatgatg ccaaaaaaaa tacctccaat gagcatcccc
     1681 aaattagtga tcgttgaaat gaaacccgct tgcgtaccac taatatgaaa agaagtaatc
     1741 attgaagata atgaaaaagc taaaaacata atatccatat tctctaaagc aattcccgcc
     1801 gatgtcgata ataataactt tcgctgatac gttgtcattt ttatacccct tttcctaaaa
     1861 tttccctaaa ttgttaaaag tatatccaaa aaagaataaa aaaacaagac ttttttattt
     1921 gaaataatac acaaacaacc gatatagata atccagctta gttttaatag aaatgagtat
     1981 tgacttggag tacacttaaa gttgtagaat aaataaaata cataagtaaa gtggcaagaa
     2041 attaagagaa agagtgatca gatgaatatc aaacaagtca gtgaagaaaa gggcatttcg
     2101 gccgatacat tgcgctatta tgaacgaatc gggttgattc caccagttaa tcggacaaaa
     2161 ggcggaatcc gtgattatac agaagaagac ctaaaatggg tcgactttac gatctgtatg
     2221 cgaaatgccg gattatcgat cgagtctttg attgaatata ttcgcctatc tagcgaagga
     2281 gaagcaactg tatttgagcg gcgtaagctt ttgattgaag aaagtgaact tttgacgaaa
     2341 aaaattgctg aaatgcaaga atgtttggaa cgtttacaag gaaaaatcaa gaaatatgat
     2401 cgtgtgttat ctagaaataa aatggaatgt atcaagtaaa aaaatcaagg agcatcgcta
     2461 tggagttacg cttactaaaa tatttttgga caattgcaga agaaggaaca atctctcaag
     2521 ccgcagaagt tctacatctt acacaaccaa ccttaagccg acaactgaag gagttagaag
     2581 aggaactagg aacagaactg tttgtacgcg aaaaccgcaa aatgatcttg acagaagcag
     2641 gttattttct aaaaaagccg agcagaagaa attttagatt taacgaatca aaccactcaa
     2701 gaattcgaga acagaaaaaa acaattattc agcggccgat ttacgatcgg ctgtgtggaa
     2761 gctgataatt cgaatacatt ggcactatta ctagaaaagt tgatccgtga ttatcccagc
     2821 gttcaatttc atatccacag cggaacaagt gatgatatca cggagtgact agaaaaaagc
     2881 ctgttagatt tagcgatttt gctagatcca gtagatacag agaaatacga aacgattttg
     2941 ttaccaagaa aggaaaaatg gggattgctc attttagaaa attctgtttt agcaaaaaaa
     3001 gacgtgataa caaaagaaga ctttgcgaat gtacctcttt tgatctcaaa gcgtccagaa
     3061 atacagcgaa tgtttgctga ctggttaggc ataaaaaagg agcagttgct gatcacagga
     3121 acgtacaatt tgattttcaa tgtatttgcg ctaattgaga atcaggtagg tgctgcttta
     3181 gtcatagaag gtgctgtaag tcatctttca aagcgagtga cattcgttcc gcttgaccca
     3241 atgattgaaa cgaactgcat gctagtctgg aaaaagaatc ggattttatc acctgtagtc
     3301 aatgaattga ttcaccgttt caaagaagct tttgaacagt aataagtatc tctaatcgaa
     3361 gaaatttcaa tacaaaagcg agacgtatga cagtcttgct tttgtatttt ttcattttta
     3421 aatcaaaaaa aataaagaga tttacataac ttgttaaata agcgcttaca aacataagaa
     3481 gaacggaaat gaatcagcta taataaccgt tgttctggat tgtaatcatt acaaagaaat
     3541 gaagattata actttggagg tgttcgaaat gaaagaaaaa aagcaaaaat tcgttcaaaa
     3601 aacatgccct ttattcaatg gttgggtatg attctgctga ttgtattagc tttattgacg
     3661 atcattcctt ttttatggat ggtttctgct tcattcaaag caaataatga agtgttcacg
     3721 attccgattc aatggatccc taaaacttgg catttagaga attatctggt tatttggtct
     3781 cgtattcctt tagtgacatt ttttaagaat acagcttttt taagcgttgt gatcacgttg
     3841 attcagttgt ttacttctag ttttgctgct tacgggtttt ctaaactaaa ttttaaaggg
     3901 cgcgatattt tatttttagc atatatcggc acgattgctg tcccttggca gtcttatatg
     3961 atcccgcagt tcattatgat gagaaaagtg ggtttaacat atacgttgtg gtcgttggtg
     4021 ctattacaag cgttcaatgc ttttggtgtg ttcttgttga agcaatacta cagtagtatc
     4081 ccagattctt tatgtgagag tgcgcggatc gatggcttat cagagtgggg aatctatcgc
     4141 aagatcattt tacctcttac aaaaccggcg attgccagtt tgacgatcat cacattcgtt
     4201 aatacatgga acgattatat ggggccattc atttatttgt cttctacgga aaataaaaca
     4261 atccagttag ggttgaaaat gtttattggg ctatttgatg cagagtacgc attaatcatg
     4321 gctgcctcag ttgtttctgt tcttcctgtt gcttttatat ttttagcgat gcaaaaatat
     4381 tttgtagaag ggatcgcttc atcaggaatg aaggaatagg aggtgattat gaaagagcag
     4441 cgttttgaca aaaatgttta tatggaattc ttttattggt tgtatcaatc attggcaagt
     4501 agcctttgtt tttggtgtgt caatatcttg tatatagcag ctgcgttcct tttgtcgata
     4561 gatggaagga atgtcttttc ctttattttt tctttacttt ttattggtcc aggttgcatc
     4621 gtgatgatag gtatgacaga agaattagca aaagaaaaaa gtttttcacc agtacgtttg
     4681 ttcataaaga cgtttcgcca attttggcta aagggatttt tttattggct atttgcttgg
     4741 atagttagtg tgataatgat ttttgattgt ttctttttta tacgtttttc ttatggaaaa
     4801 tggttgattc cgttgtttgt tttacttgct tgtttatcag tttcctttag catcaactac
     4861 tggtattttc aggtgagaaa tccagcgtcg aaaccaaatc aagttctacg gatagcgttc
     4921 tactatacgt tgaaaaaatg gtatgtgagt ttactagatt ttttactatt gacaagtttg
     4981 tttctcttct ttttcgtaaa gccccaatgg tgtattctac taggtccttc tatcgtattt
     5041 ggtttgattt acttcaacaa tcgtaaattg atgagaacta tggatctgta gactttataa
     5101 attgaaactt tcaattaaga atgacagttt ttatgtaaaa actgtcattc tttttcattt
     5161 tttgaaacat atggctaaac aaatgaaata aaacgtgcta tacttttgaa tgaaacaggt
     5221 ttagaaactg tatcaattat ttgtgattat ttctaactaa taattaaaaa taaagcataa
     5281 aaaggacgat gttcaatgga cgaaaaaatg aaacaaggtt cgaaatctgt agtagaaagt
     5341 ttgattaatc atcatgttga ttatgtattc ggtataccag gtgcaaaaat cgatggtgta
     5401 tttaatgaat tagaggatca aggtcctgaa ttaatcgtga ctcgtcacga acaaaatgct
     5461 gcatttatgg cacaagcaat cggacgtatt acaggagagc caggagtagt tatcgcaaca
     5521 agtggtcctg gtgctagtaa cttagctaca ggtttggtaa cagcgacagc agaaggtgac
     5581 ccagttttag caattgccgg acaagtaaaa cgaagcgatc tattgaagtt gacccatcaa
     5641 agcatggata atgcagcact gttccagcca atcacaaaat acagtgcaga aatccaagat
     5701 ccagaaacaa tttcagaagt gattgccaat gcttatcgta tggctaagag ttcaaaaaaa
     5761 ggcgcgagct ttatcagtat cccgcaagat gtagtggatg cacctgtcca agggaatgtc
     5821 atcaagccat tgagcgatcc taaattaggt tctgcttctg cagatgatat ccgttaccta
     5881 gctgaacgca ttagagaagc aaaactaccg gtactattag taggtatgcg aggatcaagt
     5941 gaaaaagaaa cgttagccat caggcaatta gttggaaaga cagcattgcc tgttgtagag
     6001 acattccaag cagctggagt gatctcacgt gaattagaag cgcatttctt tggtcgtgtg
     6061 ggtttattta gaaaccaacc aggagatatg cttttgaaac gaagtgatct agttattgct
     6121 attggctatg atccaatcga atatgaagca agaaactgga atgcggaaaa agatgcgcgg
     6181 atcattgtga tcgatgaagc gccagctgaa atcgatcctt ttatgcagcc tgaaagagaa
     6241 ttgatcggtg atatctcagc cactcttgat ttattgacag gttcattaga gcctcaacaa
     6301 gtgtcagaag atgcaaaaga atatttagca tctcttcaag cagaattaac ggagcgggac
     6361 attgttcaat caaaaggaga agcaggtatc ttgcatcctt tagaagttat taacacgctt
     6421 cagtcaaaag taacagatga tatgactgtt accgtagatg taggtagtca ttatatttgg
     6481 atggcacgcc actttagaag ttatgaacct cgtcatttgt tattcagtaa tggtatgcaa
     6541 acgcttggtg ttgcattgcc atgggcgatt tctgctgcat tggtgcgtcc aaatacacaa
     6601 atcgtatcag tttccggcga tgggggattt ttattctctg cccaggattt agaaacggcg
     6661 gtacgtaaaa aattgaatat cgttcatttg atttggaatg atggacacta caatatggta
     6721 gagttccaag aaaaaatgaa atatcagcgg gcgtcaggtg tagactttgg tccagttgat
     6781 tttgtgaaat atgcagaagc ttttggcgct aaaggaatcc gagctacaag tgtagaggaa
     6841 ttagaaaaag ctttagaaga aggatttgcg acagaaggac ccgtaattat tgatattccg
     6901 attgattacc gtgataatga gaaattggga gaaacaatct taccagatca attctattaa
     6961 ggaagtgtcg acatgactga aaaaatctta taccagcacg ggacattagg agctttgatg
     7021 gctggcttga tggatggaac agaaacgatt gctcatatat tagaaaaagg aactctggga
     7081 ttgggaacat tgcatgggct tgatggtgaa gtcatctttt tagatggcgt agcttatcaa
     7141 ggaagatcag atggctcagt tgcccaatta gatggaagtg aacttacccc ctatgcagca
     7201 attacagatt ttacgccaga tactagtttt tctgtctcaa aaactgcaga tggagaacaa
     7261 ttgaaaaaag acatattagt aagagaagcc ggggaaaacc tatttttggc agtgaagata
     7321 actggattat tcaaaaatat gcacatccgt atcatgccga agcaagaaaa accttacaga
     7381 agactagcaa aaatttcaga gtctcaacca gaattccaac aaagcaatgt ccaaggaaca
     7441 ttagtcgggt tctttacgcc tgaattattc caaggagttg ctgcagcggg atttcacttg
     7501 cattttatcg atgatacaca tacatttggc ggacatgtaa tggattttga agtggctgaa
     7561 ggaaaagttg agattagccg catctcgtcc cttgtacagc attttccagt tgatgatccg
     7621 acttttgttc acgaaacgat tgattatgct gatttagcag cagaaatcca acaagcagaa
     7681 tagaaaaaga cagcgagatg cgttcaaggt gattaacctg acgtatctcg tttttttgag
     7741 gaggaaacct agcgtttaca aaaaaactga gctataatct tctccataga tcaaaaagga
     7801 gcggttttta tcatgtggac agaaaaacag aaaaaagatg catacgaaat tttattatta
     7861 cagcaacaag gattattaga agcagaagat aactggttag caaatttagc aaattcatct
     7921 gcattgttga atgagacttt gcctgagact gtctttgcag gatattattt attcgatggt
     7981 aatgaattga ttttaggacc gttccaaggg cgagtttctt gcacacgtat caagatggga
     8041 aaaggagttt gtggagaatc agctgaaaaa caagaaacat tgatcgttga tgatgtcaaa
     8101 acccatgcaa attacatttc ctgcgattca gccgcacaat cagaaatcgt agtgccaatg
     8161 gtaaaagaag gtaatttaat cggtgtttta gatatcgatt gcggagtaac gaaagggtat
     8221 gatgaaatcg atcaagcatt tctagaaaaa tatgtagatc ttttattagc aaattttaat
     8281 ataaatgctt tttcatttat taataactaa aatcgtctat tgaaacaaaa aagaagagaa
     8341 atcaagaata aaaagagccg gaaagacagt gggcatttct gtttttcggt ttttttattt
     8401 ttttataaaa tcacaagtgt tattgatttt cataatcata ttttaatggt attatcattt
     8461 taaataatgg aaaaacatta aggatggatg aaatatgaat aacgaaacta tcattttagg
     8521 aaaaaaatat atttgccaac ctattggctt gaaacaaaaa gtggttggag aagtaatcag
     8581 taaattagaa aattgtgttg tgatctgcgt agacgaatat catgacgtcg atcgcaatga
     8641 aatattagaa aaatgcggaa aagtagtggc aaaatatact cacgtttatg agttggaaac
     8701 aacagaatat ctatataact atagcaacaa atatgaacat actttagttt cataaagatt
     8761 taaataaagt gccttcaaaa gcgagaagac caagcttttg aaggcacttt atttagttgg
     8821 cagtgattgt tcatgatttt tgatttattc aaaaggttcg cctagagaat ctgtctctgt
     8881 aacgaggcct ggtggagaaa ctcgataaaa acctatactg ccagaactgt cctattttcg
     8941 gatagacaaa ctagtagttt taaaaagatc atccgatcca atcatttcaa aaaatgcaaa
     9001 actgatatca ggatcattta gctcaggctt gttatcgtaa agcatttgta aaggttgcat
     9061 ttagttagga gtaaacgcga aactatcatc attatttgct gaagaagatg aagaagctaa
     9121 ttcagagttt gtttgaaata aaggagacgt gctgacagtt tctttaccct gttttttgca
     9181 gaggtagaaa cttttatatc ggaagtttct accggagaat ttttctcatt tgtagaaatt
     9241 gttgaatcga tactattcga aatttgcgta tctgaaggta tgttctttga tgcagaagtc
     9301 ccacaagcag taagtaacaa taaatttgat aaactgataa acagaattct tttcattatg
     9361 tacacactcc ttatagtatc gtttagcata cacctatgct cagtattagg caaaatcaat
     9421 gcaaaactat caagtctttt atagtatcta ttagtcttag taatacatat atggagaggg
     9481 tgatatccca gttcaaacca gatacattgg ttaccatctg tattaacgat tatgcctcta
     9541 aacgcttttt ttatgaaaaa atttataagt gaaaacgtag gtatgatatt atctagaggt
     9601 aatttttttg ggtatttaaa tagttgctag gaggatag
//
LOCUS       DAGCWU010000112         9620 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.112, whole genome shotgun sequence.
ACCESSION   DAGCWU010000112 DAGCWU010000000
VERSION     DAGCWU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.112"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            191..616
                     /locus_tag="MBZ71_002055"
     CDS             191..616
                     /locus_tag="MBZ71_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4429979.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(1104..1805)
                     /locus_tag="MBZ71_002056"
     CDS             complement(1104..1805)
                     /locus_tag="MBZ71_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBT4429980.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(1821..2261)
                     /locus_tag="MBZ71_002057"
     CDS             complement(1821..2261)
                     /locus_tag="MBZ71_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBT4429981.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(2261..2836)
                     /locus_tag="MBZ71_002058"
     CDS             complement(2261..2836)
                     /locus_tag="MBZ71_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBT4429982.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(3684..4163)
                     /locus_tag="MBZ71_002059"
                     /pseudo
     CDS             complement(3684..4163)
                     /locus_tag="MBZ71_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(4322..5023)
                     /locus_tag="MBZ71_002060"
     CDS             complement(4322..5023)
                     /locus_tag="MBZ71_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4429983.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(5131..6540)
                     /locus_tag="MBZ71_002061"
     CDS             complement(5131..6540)
                     /locus_tag="MBZ71_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBT4429984.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(6588..8453)
                     /locus_tag="MBZ71_002062"
     CDS             complement(6588..8453)
                     /locus_tag="MBZ71_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4429985.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(8574..9425)
                     /locus_tag="MBZ71_002063"
     CDS             complement(8574..9425)
                     /locus_tag="MBZ71_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4429986.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         2828 a         2067 c         1582 g         3143 t
ORIGIN      
        1 gcattttttt aaaagattgc aacacttttt taaaacgctt tctgaaagtg gcggattttt
       61 tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg tgcgtttttc
      121 tcttccatcc gtttgaagtt ttacattcca atggtataat ttctttatcc tatcaaatgg
      181 gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac ttttgacatt
      241 gactggctgt gatttgtttg gcggctctga ccaatcctcg gacgaaacaa ccgattcttc
      301 tgtcttaaaa gcggaaaaag cagataccac caacagtgta tatacgctaa aagtacgtaa
      361 agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg aatggacacg
      421 cacgtatcag acacaaggaa ctgattcttc tgaatcatct gaagaaaaaa cagtgctgac
      481 agatatcaag atcactacca aagatgacga atatattatt tcaggaaaag aaaataaaaa
      541 agaaacaaca gtcatattta caaaaatcgg taattaccgt ttgaaggatg aggatggcaa
      601 catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct tcttttctgc
      661 tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat agtcctgatt
      721 attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa ggaagcttga
      781 gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt gaatcttatc
      841 ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa actcactatg
      901 gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca tcagtaaaac
      961 aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat ttcaaatatt
     1021 tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt aatttttctt
     1081 tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg ctggttatcc
     1141 gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg gatctgacgg
     1201 atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc atccacttgt
     1261 attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt caacgtttca
     1321 taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc acgttttttg
     1381 atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc aggccgtttt
     1441 tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc gtactgctgt
     1501 acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag gctatcttca
     1561 tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat tgctaaaaat
     1621 cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt tgaataaaag
     1681 agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc aatctctttt
     1741 gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat caaaatcact
     1801 gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac aggcaatgtt
     1861 tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag actttgtgta
     1921 atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt tttcaacata
     1981 gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc aaagtcaagg
     2041 atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta tacttcctgc
     2101 gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg agagtagaaa
     2161 cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg atcgatttgc
     2221 tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc tccttcaata
     2281 ataactgatt tttaatagat actgctcgta attcattgac cagttccaat ggattgttgg
     2341 aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca aaagtttcta
     2401 ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt ggcttgtcgg
     2461 gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat gtacctagac
     2521 ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt ctttcatcga
     2581 tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt tcaaaaagcc
     2641 cattcggata gatacctgtg acttttccgt tattttccag tactgtttta ctaatcactc
     2701 ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt gccatgtatt
     2761 ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg gaaccgcaaa
     2821 aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc attctacacg
     2881 tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata ggatagacgc
     2941 cactatatat agtatttaga aaaaaatcat ttttcagcca ttttatagat aaaacaaaaa
     3001 taaataacgt gttttcatcg atatgaaatg acttacttat agaagatact gggatttcgt
     3061 cctatatgcc aataacgtgt attattttaa acaacaaata cttgtttctt ttattaacga
     3121 aaaaatcatt ttgttctcgt gttatgtttc actgataaat gatgtttctc taaataaaaa
     3181 agccgacgaa ctaaacgatc aaaatttgat catttttgtt catcagcttc tcctaaaaat
     3241 ctttttgtta acgataaata gctggatttg taaaaatatc acttgaatcg tcactgctgg
     3301 aagaaaattc agtgataacc gcacctgttg cgcctgcctg gaactagtgt ttgatattgg
     3361 gctcgaacgt gtactgatct ctagcatgaa gaataatttc tttttttact gtataataac
     3421 tttctttcct ttttggaata gtacaggaaa gtttttttgt ttcctttcct tcgacataag
     3481 gtataccaga ccctttcgac aacgaaaggt tctcattttt ccttcatcgt catttcaagg
     3541 agggtgccga tgctctggac agtttcgatc gctcctaaac caaaatctgt atattcgatt
     3601 ctttctaact ctgattcttt cataccattc ctgtctcaag gtacatctat tgtacttttt
     3661 tcttgtaatc cattctgcct ctcctaacta attgtcaaat catcccaaga tatgtcttct
     3721 tcctcttttt cttcttggat gatttctgca ttcggatcta ctggtttttt caataataaa
     3781 atggttattc ctgtgacaaa agaaccagct aacaatgcac caatgaatgc ccatggtctg
     3841 gtcatcgtag cgactgcaaa cattcccgat gccggaatac ggctatctgc gccccaaaac
     3901 atgcttaatc cgccacctac tgtcccaccg actcccgttg ctatgacatt gcgtgccaag
     3961 tcgttcaata cgatagggaa acatccctct gtgatctgac agatccccat aggaaaagcg
     4021 gtcttcaatg tatcgatttc ttgacgattg agtatatttt tacgaatgat tttccctaag
     4081 aaataagcga tcgtcaagcc gaatggggtt accatcgaag ctaaaatcaa tacagtgatt
     4141 ggttcataga ttccttctga aaatcgtgcc acaccccctt atccagaata aatggtaatc
     4201 cagaaacagc cgtgtatctg cggtaataag cctaaccaaa cgaaaaatat gagaagctat
     4261 ttttgtttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttttttta
     4321 tttatgtttc ataatcaata tggtaaggat gcagctccgt atggtagacc gcctccgaat
     4381 attcaatcat ttccccaatt tgcgtagacg atttccgtat ccgtttgatg ccgatctgtg
     4441 cgtctgtttt cagaattcct tgttcttctt ctgttaaaaa tactgccgag aaatcatcag
     4501 aaaatgagtc aatagtatat ccttttttcg tcagcaaacg atacaaagat tctttgccaa
     4561 attcttctct gctgaattgc atcatttctt gtggaagata atgaacaaaa taaatataag
     4621 gctgattgtt caaataatat aagcgcttaa aacaaacagc ttgctcaccg aaaaaggaaa
     4681 acaaaggatc ttccttcttt atatggacca attgcaaatc cagcgtttct ttccggactt
     4741 catatcggga gttctccaag atttgcgtaa agcttgccgc tttggataat cgattgtaag
     4801 gacgattgct tagaaccgtc gtcccacgac cacttttctt tgcgacatat tcatctgatg
     4861 ccagcagttc gattgccttt cggacagtga ttttgctgac ttgaaacagt tcttctaatt
     4921 cactttctgt cggcaacatc gaaccgactg gatattttcc tgagaaaata tcttctttaa
     4981 ttttatctgc gacttccaag tatagtacac gtgtcctttt cataacgtca ccctttgctt
     5041 tactattact aaaagaatag cacataaaaa ataaaaaaag aacacaaatc acatgtgaga
     5101 agtgtgttct ttttcattca gctgctttaa ttacaagttt acgccatttg aggcaattac
     5161 ttctttatac caatcaaaag attttttctt tgatcgattc agtgtcccat ttccttcatc
     5221 gtctagatcc acatagataa agccgtaacg tttactcatt tctcctgttg atgcactgac
     5281 aagatcgata catccccaag gcgtatagcc catcaaatct acaccgtctg ctactgcatt
     5341 agccatcgct tcgatatgac gacgtaaata atcgatccga taatcatcaa taatcgtgcc
     5401 atcctcttcc atatgatcaa tggctccaag accgttttca acaatgaata atggtttctg
     5461 gtaacgattg tataattcat tcaatgcgat tctcagccct tctggatcga tctgccagcc
     5521 ccattcactt gcctcaagaa acggattttt tactcctccc agcaagttcc cttctgaagt
     5581 tgctgcttgt ggatcagtct cattgattgc cgtagacata taataactaa atccgatgta
     5641 gtccactgga tattctttta gcaactggaa gtctttttcc gtttgctcta atttttccgg
     5701 atcgactcca tattttttat gggtacgcgc tgtgtatgca gggtattctc cacgtacttg
     5761 gacatccgtg cagaaaaaat tgaattcttg gttttggatc attgttgctg cctgattgac
     5821 tggattggca tcgatgctgt aagttgttgc atagataatc atgcagccga tttggatatc
     5881 cgaacgcaat tcgtggccga tttttacggc tttgctactt gcaacgaatt gattatgcca
     5941 tgcttggaaa atattttggt actctcctgc tccattgctt tttactaatc cttgacttaa
     6001 agctggaaag tgaaaggccg aattgatttc attgaaagtc atccaatatt tgactttact
     6061 gctgtatctc tctaatactg tctgagcaaa tcgttcatag aagtcgatca gttttcgatt
     6121 tttccagcca ccgtattctt ttgccaaatg aagaggcatt tcgtaatgag aaatcgtgat
     6181 cacaggttca atatcatatt ttaagcactc atcaatcagc tgatcataaa attctaatcc
     6241 cgcttcgtta ggagtacctt cgtctccatt tggaaaaatt cgtgtccagg caatcgaaaa
     6301 acgataacat ttgaatccca tttccgcaaa caaggcaaga tcttctttga aacgatcgta
     6361 atgatcgatt cctcgatgat tcgggtaaat atatttttct tgatcaatcg tccagtcaaa
     6421 ttcttcactg ccgataatcg caaagcgctg cttacctcca ggcatagcat ctgcaacgga
     6481 tagtcctttt ccgtctcgat cataagcccc ttctaattga tttggcagca gttgctccac
     6541 cccataaaaa tttttctgaa aaacgtgatt gcatcctgat ttcctcctta ttttacaatt
     6601 gccagaacat cttcgtttgc aataatctct ttttggtcta attcaagtat atcggcaaat
     6661 gcatcagtat tcgtaatgac aactggagtg attacgctat agccagctgc tctaattgct
     6721 tcgatgtcaa aacgaatcaa taaatctcct gctttgacga gatccccttg tttgactaag
     6781 atctcaaagc cattcccatt taattctact gtatccatcc cgatatgaat caaaatctcc
     6841 gctccgtcag tcgttgtgat ccctacagca tgtccagttg gaaaaatagt cgtgatctct
     6901 ccgtctgcag gtgcaactag ttctcctact tctggatcaa tagcgatacc ttttcccatc
     6961 acgccagaag aaaaaacttg gtctggtact tcactcaatg gcagtacttt acccgttaaa
     7021 ggacttttta aggtatgccg tgctgaagta atcggttctc ctgcattcgc atgtttgttt
     7081 tctaattgat tgtcttgtgt ttgttcgtca aatcctaaaa tcaatgtacc tacgaatgca
     7141 agaacaaaag caattcctgt agctattact gctactgtca catttgactc tacaccatcc
     7201 accgtattaa tgaaagtcgg aatcgtcaat aagctgacca aactgctgga gaaagctttt
     7261 actccggaaa atccgataat agctccacca atacctccag aaatacaagc ggcaatgaat
     7321 ggtttcttca aaggtaatgt cacaccatac acagttggtt cagtgattcc aaataacgaa
     7381 gtcactgtag aagaaatccc taatgctcga agtttcgtat cttttgttcg caatgctaca
     7441 gctaaagcag ccccaccttg tgccaagatg gctggaagaa gcattggcat caatacatca
     7501 aacccatatt gttcgatatt caaaaacatg atcggtacga atccccaatg catgccgaac
     7561 atcacgaata cttgccataa acttcccatg attagacctg ctacgattgg actaaatcca
     7621 taaatcgagt tgaagagacc gcctaaagca ttaccggcaa ccgtcccgat tggtcctaaa
     7681 gcaagaaatg ttaatggaac catcactact aaaacgacaa gcggaactaa aatcatttgc
     7741 agaaattgcg gaatcacttt ttttacgaat ggctctaatt tactttgaac ccatacggct
     7801 agaatgattg gtatgacaga agaagtataa ccagatggtg aaagaatcac tgggatccca
     7861 aagaaactga tagcttctcc cgcaccagca actgctgtga tagctggata cacaagtgcc
     7921 atagcaattg ctactgctaa aaactggttg gctttgaact ttttagctgc tgtaaaagca
     7981 agcatgacag gtaagaaggt aaagacacca tctgctgcag caaacaatat ttgataagta
     8041 ccgctatcag ctgtcagcca gcccatcacc gttgccaatg ataagaatcc tttcaatacc
     8101 ccggcggctg ccatcgcccc tagaaaaggt gtaaatatac tagagataat atcgatcaga
     8161 cgattcaaaa tatttttgga ctcttctttt ccatcctccg aaactgcaag accttcctca
     8221 tccacgatcg attgataaac atctgctaca ttactgccaa tcactacttg atactgaccg
     8281 ccactttgta cgacttggat cacgtccggg tcttctttta acttttgcgt atctgctaaa
     8341 gattcatctt ttaatctgaa tcgcaaccgc gtagcacaat gtactaaact tttgacattc
     8401 ttttgtcctc ctaccgcttc ccatacacga cgaccaactg cttgattatc cattgttttt
     8461 tccttctttc ttttttattt aaaaaataaa aacccagatt gaaaaaaaca gtcacaaaca
     8521 ttcgtgaacc tattttttca atccaggttt tgcctgccat acagtaacaa tccttatgat
     8581 tggatgcttt ttttctcact tactactctt tgaatatgaa tcgttaaata aatctgctct
     8641 gcgatcccca tttcctgttt ttgcgtttgt cgaagatact cattgatccg ttcgacacaa
     8701 tgaaacgctt ttggatactt tccttgaact aatgcgtaga gaaattcatc ttgctcgtca
     8761 gatccttccc ctttgagata acgctgggca aaaaactgca gatgagtaat gatacgctga
     8821 taattcaaag attcttcatc gaacacgatg ccgaagaatt tactgatgat actcagtata
     8881 tctcttacca ttgtggtagc ggtcatcgtc acttgcatgt cttcgtttcc ttgttggcta
     8941 ttcacaaaat gaaaggcaat aaaaccggct tcatcctcag aaagttccac acccgtcttt
     9001 tgtttcacga acaaaagagc ctctttcgct gcttgatatt ctttcggata aaattttttt
     9061 gtttcaaata ataatggatt aggcagttgg atattttctt ttgctcgtgt gatcgcataa
     9121 tgaagatgat cggtcaatgt cagataaata ttaggcgaaa attcaacttt tagtttatct
     9181 tcagccagtt cgactacttg tttgacccat tcgatctcct ctgccggaat atcagccatc
     9241 agctgctgga attggttttt agcaaaagaa tctcttaaaa caaattcttt ttggaccgct
     9301 tctggcgaaa tctcgtcccc aatctttttt tgaaaagcta gtccttttcc catacaaacg
     9361 acttcttccc cgaatccatt tttcgaaatg acgacattgt tattcaaaat tttatcaatt
     9421 ttcattttgc ttcgccctct caagaaaaat aaaaaaacct agaattcgcc tactgtttct
     9481 tagctgaatt ctggttttgc ctgccgtaca gtaacaatcc atttcttatt caaaatcatc
     9541 ataccatcga atttttattt atgcaagcct tttcatataa aaaataaaaa aactggaaca
     9601 caagtctatc tagaaaacaa
//
LOCUS       DAGCWU010000113         9540 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.113, whole genome shotgun sequence.
ACCESSION   DAGCWU010000113 DAGCWU010000000
VERSION     DAGCWU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.113"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            147..1016
                     /locus_tag="MBZ71_002064"
     CDS             147..1016
                     /locus_tag="MBZ71_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBT4429987.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="MBZ71_002065"
     CDS             1225..2250
                     /locus_tag="MBZ71_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBT4429988.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="MBZ71_002066"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="MBZ71_002066"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBT4429989.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="MBZ71_002067"
     CDS             3333..5036
                     /locus_tag="MBZ71_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4429990.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="MBZ71_002068"
     CDS             5038..7071
                     /locus_tag="MBZ71_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4429991.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="MBZ71_002069"
     CDS             7082..7522
                     /locus_tag="MBZ71_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4429992.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="MBZ71_002070"
     CDS             complement(7722..8849)
                     /locus_tag="MBZ71_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4429993.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="MBZ71_002071"
     CDS             complement(8852..9529)
                     /locus_tag="MBZ71_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4429994.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
BASE COUNT         3236 a         1652 c         1953 g         2699 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
//
LOCUS       DAGCWU010000114         9537 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.114, whole genome shotgun sequence.
ACCESSION   DAGCWU010000114 DAGCWU010000000
VERSION     DAGCWU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.114"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            99..746
                     /locus_tag="MBZ71_002072"
     CDS             99..746
                     /locus_tag="MBZ71_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBT4429995.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="MBZ71_002073"
     CDS             1139..3784
                     /locus_tag="MBZ71_002073"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBT4429996.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="MBZ71_002074"
     CDS             4094..5407
                     /locus_tag="MBZ71_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBT4429997.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="MBZ71_002075"
     CDS             5397..6050
                     /locus_tag="MBZ71_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4429998.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="MBZ71_002076"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="MBZ71_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBT4429999.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="MBZ71_002077"
     CDS             6947..7147
                     /locus_tag="MBZ71_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4430000.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="MBZ71_002078"
     CDS             complement(7401..9080)
                     /locus_tag="MBZ71_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBT4430001.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="MBZ71_002079"
     CDS             complement(9082..9294)
                     /locus_tag="MBZ71_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBT4430002.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
BASE COUNT         3038 a         1701 c         2035 g         2763 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt atcttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaa
//
LOCUS       DAGCWU010000115         9495 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.115, whole genome shotgun sequence.
ACCESSION   DAGCWU010000115 DAGCWU010000000
VERSION     DAGCWU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.115"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            8..1195
                     /locus_tag="MBZ71_002080"
     CDS             8..1195
                     /locus_tag="MBZ71_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBT4430003.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1176..2480
                     /locus_tag="MBZ71_002081"
     CDS             1176..2480
                     /locus_tag="MBZ71_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBT4430004.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2506..3519
                     /gene="citC"
                     /locus_tag="MBZ71_002082"
     CDS             2506..3519
                     /gene="citC"
                     /locus_tag="MBZ71_002082"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBT4430005.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3523..3816
                     /gene="citD"
                     /locus_tag="MBZ71_002083"
     CDS             3523..3816
                     /gene="citD"
                     /locus_tag="MBZ71_002083"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBT4430006.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3821..4726
                     /gene="citE"
                     /locus_tag="MBZ71_002084"
     CDS             3821..4726
                     /gene="citE"
                     /locus_tag="MBZ71_002084"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBT4430007.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4719..6278
                     /gene="citF"
                     /locus_tag="MBZ71_002085"
     CDS             4719..6278
                     /gene="citF"
                     /locus_tag="MBZ71_002085"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBT4430008.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6275..6832
                     /gene="citX"
                     /locus_tag="MBZ71_002086"
     CDS             6275..6832
                     /gene="citX"
                     /locus_tag="MBZ71_002086"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBT4430009.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6825..7688
                     /gene="citG"
                     /locus_tag="MBZ71_002087"
     CDS             6825..7688
                     /gene="citG"
                     /locus_tag="MBZ71_002087"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBT4430010.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            7922..8331
                     /gene="tnpA"
                     /locus_tag="MBZ71_002088"
                     /pseudo
     CDS             7922..8331
                     /gene="tnpA"
                     /locus_tag="MBZ71_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(8968..>9495)
                     /locus_tag="MBZ71_002089"
     CDS             complement(8968..>9495)
                     /locus_tag="MBZ71_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4430011.1"
                     /translation="FEYLKERGLQGTELVISDAHKGLVSAIRKSFTNASWQRCQVHFL
                     RNIFTTIPKKNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDG
                     FEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNCVSANRLIGAVLIDQH
                     DEWLSSSRKYIKFAK"
BASE COUNT         3276 a         1485 c         1923 g         2811 t
ORIGIN      
        1 tgaaggtatg cttgatgaag ttttagaaat ccataaaaaa aatgtaggtg ttttacggat
       61 ggaaacaaca gaagccgtgc aagatgatca agatttagca aaaatgtaca ccccaggtgt
      121 agcagaacta agtttaatga ttgagaaaaa tcatgctttg gcaagagaat taacaattag
      181 tggtaaatta gtggccgtta ttacagacgg gtcggcggta ttaggtcttg gaaatgtagg
      241 aaaccaagct ggattaccaa tcgtcgaagg gaaagcgctg ctttataaaa atttagcagg
      301 tgtcaatgcg attccattag ccatcgaaca aaaatcagta gacgagattg tacaaacaat
      361 tgtcaatttg caaaatagtt ttgcagggat ccatttagaa gatattgcag caccgaagtg
      421 ttttgagatt gaagaaaaat tacaagaaaa actttctatt cctgtgtatc atgacgatca
      481 agaaggaaca gcgatagttg ttttagctgg attgatcaat gctgctaaga tccaaaggaa
      541 aacattaact gaacttcgag ttttgatcaa cggcatggga gcatctgggg ttgcaacagc
      601 tagactatta atcgctgctg gtatcaaaaa tctaacattg gtggataaac aagggagatt
      661 aaagaagcaa gatagtacat taaataaata tcaaagagag ttgttggatc atatacatga
      721 tacaaataat aatgcaagcg ttttggaaga tgcaatcgtt ggacaagatg tttttatcgg
      781 tctttcaata ggaaatgttt taacgcctga aatgattgga aaaatgaatg ataatccgat
      841 tatttttgct ttagccaatc cagtaccaga gatcacaccc gatttagcaa aagaatctgg
      901 tgcaaagcta atcgctacag gttcttcgaa atatcctaac caagtaaaca atattttagt
      961 atttcccgga ttgttcaagg gactattact atcgaaagcg aataaagtcg atctttcatt
     1021 acaacttgaa gtagccaaac aattagctgc taaggtcgca gagcctacgg tagaaaaatt
     1081 catccctaat gtttttgatg aaggagtcgc aaacgtcgtt tcacaggcag ttttggatta
     1141 tttacaaaaa ctaaaagaaa acgaggataa tatacatgct gaaaacatta aatagaatta
     1201 aaattggttt tattccttta tctctttata tcattttggt actcgcgtat cttgcaatga
     1261 cgataagcaa tatcgttcca gaaaatgata tgttagtttc tttgggtatt atgactgttt
     1321 ttgcttattt attagaagaa attggtaaaa ctattcctat tttaaaagat ttaggtggta
     1381 aagtattagt tgttacattt ttgcctgcat ttctagtcta taaacattgg ctacctcaaa
     1441 gtactgtaac tgtggtttct gattttatga ataataacaa tttcttgatg ttctttatca
     1501 cactactagt tgtcggcagt attgcttcaa tgaatcggca aacattggtt aaagcaactt
     1561 cacgtattat cattatttta attttatgcg atgtgattgg ttctttgata ggaacaggtg
     1621 tgggaatact ccttgggcta tctgcttgga atgcgtattt ctttatcgtt gcgccgatta
     1681 tggctggtgg tgttggggaa ggagcgatgc cattatcgat tggttatgca gcaatattat
     1741 ctggtatggg acaaaatgat atctttggga agattctacc gtgtgtgatg ttgggaagtt
     1801 tagtcgcagt tctactgtct ggtatgctca aaaaaattgg agagaaatac ccacaattaa
     1861 atggtcaggg taatttgatc gatggggatg atgaacattt agagaacttg aagggttcag
     1921 ctaaaccagt caatattgaa aaaacattgt tagcaggcgt ggtagcaata acgatttatt
     1981 ttgttgctgt ctatgttgag cacttattaa aatcactcct tggcttttct attccttcac
     2041 ctgtgatctt attagtctct cttatggggg ctaaaatgct tggttttatc cctaaaaata
     2101 tcgaagaagg tggaaattcg ttgtatggat tcactgttcg tgggatcaca cctcccttac
     2161 tttttggcgt aggggttgca aaaacagatt ggttagagct gatcgaagta tttaaaaatc
     2221 ctgcaacgat agtaacaatc attgtgacgg tagtagctat tgtcgctaca gcttttatcg
     2281 ccgcaaaatt tttaaaaatg tacccagttg atactgccat cgcagtttca tgttgtagcg
     2341 gacaaggcgg tacaggtgct ttagctatct tagctgcggg tgatcgcatg gaattaatgc
     2401 catttgcaca agttgctgtt cgactagggg gtgctatgac cgtaacgttc gctatctttt
     2461 taatgggtct gctatcataa gaacataaag aaaggacgtg accatgtgtt tgatcaaaca
     2521 gatatccaac gattattttt aaaaaataac tatatcaaag accaatggga aaatcttttg
     2581 cttgattcag gaatacaaaa gaagcaaatc gaagattttc aacatttaga tcaaacattg
     2641 ggtatatacc ataaagaaaa gttagtagga acagtatctt atcaaaacaa cgtgattaaa
     2701 tatatcgccg tgtctgaaaa atataaagat ggtggacata catttaatgt actagtctct
     2761 gcaataattc aggcaatgag tcaacaagag atttttcatt atattgtttt cacgaaaccg
     2821 cgctatcaaa aaagttttaa ctttttaggt tttgaaacaa tcgtatctac cccaaaaata
     2881 gctgtattag aaatgggaga tagttctata aaacagtacc tagagaggat tcctaaagtg
     2941 aatggtgaaa aaaaagcagc aattgtcatg aatgcgaacc catttacaga agggcattat
     3001 tatttagtaa aacaagctgc caataacaac gattgggtat atgtatttgt actcgaaaaa
     3061 gaacaaaatc ttttttcgac gaaggaccga ctcaaattag ttcaacaagg attacaaaaa
     3121 ctgaaaaatg tcattgttct atcaggtggc gagtatatta ttagccctgc aacgtttccc
     3181 acctattttt tacgagaaga tgatgaaata gcaaaggagc aaatggtcgt agatgctact
     3241 ttattcaaag aaagaattgc cacttattta gatatctctg ctagatatgt gggagaagaa
     3301 ccactttccc caatgactcg ttcatataac gacatactgg aaacgatttt accccctgaa
     3361 ataaaagttc atgtccttgc aaggaagaaa acggaacaac accaagttat ttctgcttct
     3421 caagttcgta aagcgtatct tgctgggcaa ttggaaaaaa taaaatatat ggttccagaa
     3481 acaacctatc aatacttaaa aaataaaagg gagcgttaag taatggaaat aaataaatca
     3541 gcgattgctg gaacattaga gtcaagtgat gtccaaatca tgatttcacc gaatacagaa
     3601 ggaatagaaa tcgatttaga cagtcaagtg attgagcaat ttggaaaaca aatcaaaaaa
     3661 gttatctatg acacactagc agtttatcag attaaaaacg taaaactcaa ggtagtagat
     3721 aaaggggcat tagattgcgt tattgttgca cgattaaaaa cagcgatcca tcgttcctta
     3781 ggtatccaac accaagaaac accgtgggag gtttaattag atgatgaaag aacgtttgag
     3841 aagaacaatg atgtttgttc caggaaataa tccagcgatg atcaaagatg caggtattta
     3901 tggtgctgac tcgattatgt tcgacctgga agatgcagtg tctatggcag aaaaagacag
     3961 cgcaagaatg cttgtggctg aggcattgaa aacagtggat tatggtgaca cagaattaat
     4021 tgttcgaata aatgacctgc actcagaatt tggacgtgca gacatttttg caatggttaa
     4081 agccggcgcg catgtattac gtttacctaa aacagaaacc tctgaagata taacagaagt
     4141 agaaaaagtg attatagaag cagaaaacta tcataatgtt ccgatagggc gaacattgat
     4201 gatggcagcc attgaaggtg cttctggtgt attgaacgca cctgcgatcg ccaaagcatc
     4261 tgatcgattg attggaatcg ccattggggc agaagactat gtgacaaata tgaaaaccaa
     4321 acgatatcca gatggaaacg aattgttttt tgctcgcagt atgatcgttc atgctgcacg
     4381 tgcagcaggc atttccgcat ttgacactgt ttactctgat gtggatgata tggaaggatt
     4441 tttaaacgaa gtacgtttaa tccatcaatt aggttttgat ggaaaatctt gtatcaatcc
     4501 acggcaaatt ccagtagtca atactgttta tcagccgtca atcaaagaaa tcaaacgatc
     4561 actagacatc atctcggcaa tcgaagaagc aaaagaaaaa ggttctggcg taatttcttt
     4621 aaatggaaaa atgatcgata aaccaattgt agaacgtgca caacggacat tgatgttagc
     4681 aaaagcatca cgattgattg atgaggaggg aaatatccat gcataataaa caaagacgta
     4741 gcattccatc aagtgtttta acaaagtata aaaaagaatt ccaaagtgtt tcattatcgc
     4801 cacaaacgat tcaacgttgt gcaccaaaag taacgactgt aactaatcag acaaataaat
     4861 tgatgcactc gatcaaagaa gctatagaaa aatcgggctt aaaagatgga atgacgattt
     4921 catttcatca tcatttgaga gaaggtgatt ttgtttttaa ccaagtgatg gccgtgatcc
     4981 gtgaaatggg catcaaagaa ttgaccttag caccaagttc tttaacgaac actatgaatg
     5041 agatggttat tgaatgcatc aaggacggta cagtaactca tatcacaagc agtgggatgc
     5101 gcggcagctt aggtgaattt atttctcatg gaggaatgga caatcctgtg attttacgtt
     5161 ctcatggtaa tcgtccacgt gcaattgaaa gtggtgaatt gaaaatcgat gttgcatttc
     5221 taggagtacc aacatctgat gattatggaa atgctaatgc gactgttgga aaatccgttt
     5281 ttggttcatt agggtatgca aaagtagatg cacaatatgc agataaagtc attcttctaa
     5341 ctgatcattt agttccttac cctaatacgc ccattagtat tccgcaaaca caagtcgact
     5401 atgtcgtgga agtagaagca attggtgatc cgaataaaat cggtgctggt gctacacggt
     5461 ttactaaaaa cccaaaagaa ttaaaaattg ctgaaatggt caaagatgtc attgttcact
     5521 caccttattt caaagatggc ttttcttttc aaacaggaac gggtggtgca gctttagcag
     5581 taacgcgtta cttagctagt gaaatggaag aaaaacagat caaagcttct tttgcattag
     5641 gtggcatcac taaaccaacg gttgatttat tggaaaaagg tcttgttgaa aaagtgttag
     5701 atgtccaaga ctttgacaaa ggagcagctg aaggaatggc gcggcttgag aaccaacagg
     5761 agatcgatgc ttccttttat gctgatccaa gtaataaagg agcggttgtt aaccaattag
     5821 atgtcgctat tttatctgca ttagaaattg acacaaactt caatgtaaat gttatgactg
     5881 gttcagatgg tgtgctaaga ggtgcgattg gtggacatca agatgcagca acggctaaac
     5941 ttactatcat ctcagcgccg ttagtacgtg gccgaatacc tacagtagta aatgatgtta
     6001 ctacattgat cacaccaggg aaaagtatcg atgtattagt cacagaagta ggggttgcta
     6061 tcaatcccca acgtaaagat ctaatagcta tttttgaaag aattccacaa attcctgttt
     6121 ttacaataga agaattacag caaaaagcag aaaaaatagt aggcatcccg gaacctttgc
     6181 aattcacaga tcggatagtt gcctatgtag aataccgaga tggttcaatt ttagatgtcg
     6241 ttcgtcaggt gaaagaattt gaagaagaga gatcatgatg tttgatactg gagaaattca
     6301 agaaatcgag gatattttga tccataaaga acaacgtgct aacttgcaaa gcaacctgct
     6361 ttccaagtat tcaaatagtt cattgatggt ctttacattg aatattcctg ggccgatcaa
     6421 aaataatcca atgattgagc aggtatttcg tcagggacag cagtcaattc agcaaatgtt
     6481 tttaaaaaaa aaggtggaaa tgacgtacac aaaagaaatc aatttgccaa gtggattgga
     6541 ctggtacgta atagtaacag aagcaccttt gaatctcaaa aaatggttgg tcacgttaga
     6601 agagcaagac gcattaggta gactttttga tttagatgtc ttgttcaaaa aaggagagca
     6661 gataacaatt gtcagtcgcc aagctcttaa tcatggggaa cgtccttgtt ttatttgtgg
     6721 gaaatcagca aaggtatgtg cacgtaatcg tacacatact gtgaaagaaa tgcaaaagaa
     6781 aatcgaacaa atagtagccg attccttaaa aggagcattg aaacatgcat gataatattc
     6841 aaaaaatagc acaagaagca caattagcga tgctttatga agttacatgt atcaataaac
     6901 caggattggt tgatccagta gaccccgggt ctcatcaaga tatggacatt tttacttttc
     6961 tacaaagtag tgtagttttg actccctatt ttgaagaatt tatccaaaca ggattggacc
     7021 gacaacatca accaattgaa gaaaccttta tggcgattcg acagattggt ttagaggctg
     7081 aaactgcgat gttctcagtg acaaatggta taaatacaca taaaggtgct atcttttcat
     7141 tggggatttt tcttgctatt tgtggccggt taactatttg gaaagttcct tgtaaaaaaa
     7201 gtttctttca aaaagaaatt caaaaaatga cgaaaaatct acttaacgat tttgggaaga
     7261 ttgatcaaac aaaaccaaaa gcatggactt ggggagaata tctatttgtt aaccatggat
     7321 tgacagggat acgaggggaa gccaaaagag gttatccttg tgtttttgac cgaggattgc
     7381 cttattatca agcatatcaa gggtcgcaac aagataagat gatagacact ttactttttc
     7441 tatcaatgga agtagaagat accaatttga tcaaacgaag taataataga catgtttttg
     7501 aagagtatca attacttatt cgtccttatt ttgaattagg gggtgtaaaa accatacaag
     7561 gtaaaaaata tttggattac ctaaatcagc aatttaaaaa gaaaaattgg agtattggtg
     7621 gaagtgcaga cttattgatc cttacaatct ttttagataa aatcatggac aaaggatggt
     7681 tatgttgata ctataataca ttattttgta ttagtaagct aataactaaa aaatggttca
     7741 atacaaatat tttataaaaa atggtagtgc ctatcctttc agattgtaga caaagaaaaa
     7801 tttctttgtc tacaattttt ttgttaatga cttcagtcag tttcgtgcgc aaagcgtgcg
     7861 aaacaagaaa accatgcgtt tctacacgtg gaagaactag catatcgaaa ggagtttttt
     7921 catgtcaaat gacgataaaa gtttagccca tactcgttgg aattttaagt atcatattgt
     7981 atttacacca aaatatcgac gaaaagtaat ctataggcaa ttacgaaaag atattggaaa
     8041 aatacttagg aagttgtgca aaataaaaaa cgtggaaatt atagaagcac atgcaacgcc
     8101 agatcatatt cacatgcttg ttcaagtacc atcaaaaata agtatatcca gttttatagt
     8161 tatctgaaag atcgtagtgc tgtgctaatc cacgagcagc acgtaaatca caaatataga
     8221 tacggaaata aatcattttg gtcggaggaa tattatgtga gtacagtcga attgaatcaa
     8281 aagacaattg aaaaatacat ccgtgaacaa gaagctgatg atcgcatttg agatagtata
     8341 agtaaaagag agtagatgtt gcaataacaa aagggtaaaa atggactacc tccagtaaaa
     8401 taccgaaaaa tagtttaact tttggagata agtccattta agggtctata gaatgacaat
     8461 ttttttaggg gattttacta ttattacttc aagacccgct tggattcgca aagtgagtga
     8521 actcttaaaa ctacctgata gtaggttgtt ttaagagtta tctgaaagta aaaattgcat
     8581 ttatattttt gataaatacg taaatttata tatacgtatt tatcaaagtt ttaggccaaa
     8641 ggaatattgt gtaaatacac taggattgaa tgaatagacc agaagaaatt gcgtccaaga
     8701 acaaaagaag catgatagtg ctctatataa attgggcatt caagaatctg aaatttaaag
     8761 gtagaatttt agttagactt ttagatttta aacacaaaaa taaccataca agcaacaaga
     8821 agcaaagtgc tatattataa attgtccgtc cgtttactta cataagatca ttggggctat
     8881 ttttaaaaga gaataataga taataaatga aaaaaaagtc aagtccacaa tcctgtgtaa
     8941 aatactatac aatgctctca ctatttttta cttggcaaat ttaatatatt ttcttgatga
     9001 agaaagccac tcatcgtgtt gatcaataag gactgctcca attaaacgat tggctgaaac
     9061 gcagtttgga aagatacgaa taattttttc tcttctgcgc acttcttgat tcagtcgttc
     9121 aattagattg gtactcttta gtcgattgtg ggaatttcct tgtacggtat attgaaaggc
     9181 gtcttcgaat ccatcatcca atgatgcgca agcttttgaa tattttggtt gatcgatata
     9241 atcatgaatc aatcgatttt tagcctcacg agctaagtta atatctgtga acttaaaaat
     9301 tcctttaaca gcttctctga aagattttga atttttttta ggaatggtgg taaagatatt
     9361 tcttaggaag tgaacttggc atctttgcca acttgcgttg gtgaaggatt ttctaatggc
     9421 agagactaat cctttgtgcg catcagaaat aacgagttct gtaccttgta aaccgcgttc
     9481 ttttaggtat tcaaa
//
LOCUS       DAGCWU010000116         9351 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.116, whole genome shotgun sequence.
ACCESSION   DAGCWU010000116 DAGCWU010000000
VERSION     DAGCWU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.116"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            428..1231
                     /locus_tag="MBZ71_002090"
     CDS             428..1231
                     /locus_tag="MBZ71_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4430012.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1815..2270
                     /locus_tag="MBZ71_002091"
     CDS             1815..2270
                     /locus_tag="MBZ71_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430013.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2389..3447
                     /locus_tag="MBZ71_002092"
     CDS             2389..3447
                     /locus_tag="MBZ71_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HBT4430014.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3489..5438
                     /locus_tag="MBZ71_002093"
     CDS             3489..5438
                     /locus_tag="MBZ71_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBT4430015.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5441..9082
                     /locus_tag="MBZ71_002094"
     CDS             5441..9082
                     /locus_tag="MBZ71_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430016.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         3379 a         1337 c         1928 g         2707 t
ORIGIN      
        1 ttaagatttt gaaataagag aggagtaatc ctcctttatt aaatatctgt taatatatct
       61 gttaaatatc tgttaatata tctgttaggg gaggcttaat ctcttgggag agtaagtatt
      121 aaaggggtat aaaggtaaca cttttggaca gtaaaggtaa caaaagtgga cagtaagggt
      181 aacagttttg gacagcaaag gtaacaaaag tggacatttg aggtatcaca ctaggataag
      241 acgttacctt cggttttctt gatttggaag gcgaaaaaat agaaaattag attagtaaca
      301 aatctttgtt gactgttacc tttagttgtg atttaattta ggtaacaagc agaaaaaatt
      361 gtttccttta attagtacca gattaacatg attaaaggag cgcaaaaaga agattggaga
      421 gtaatcaatg tctcaagaag ttaccgaaaa gattattgat agtgatgtag atttagtatc
      481 agaacaaaca aatgcaattg tgcgttataa caatggtctg aatacaacag ctttgcgagg
      541 atttaagcct gttgaaatgg atgtgttttg ggctatttta tcaaaaatga aacgtcaagg
      601 aacagcagaa gtaacttttg atttcaacct ctttcgttat ttggtcaaat acgacagaag
      661 agaaaaggac agtttttatt ctgtgttaaa agatttgagt gacaaactag gtaatttaac
      721 ctaccgtttt gaagattcag acgtctatga acagctgtgg ctttttcaaa aattccggat
      781 agataaacag aaggaaacag ttacaattaa ggcaaatgag gaatttgaat ttatcttaaa
      841 ctcaattggc aaaaacttta ctcgttttga acttgataat tttacaaaac taagaagtag
      901 ctacacaaaa gaattgtatc gtcatctcat gcaatacaga aataaagagt ggcgaaatgg
      961 ttattgggta gtcaaagttg aagattttag aagagcatta agtatacctt caagttatag
     1021 aatgacaaat attgataaaa gaatactaga gcaggcgaaa gaagaatttt tagctcctga
     1081 tgaaaacgga atacaggtat ttgaaacttt tgattatgaa aaaatctatg caaagaaagg
     1141 aagaagagtt gatcggctca gattcacttt tagtgagcca gaatcaaact tgccaaccat
     1201 ttcaatgcat aattggttag aagaagattg agaagaagca ggttgtaatt ttaatacaac
     1261 ctgcttcttt aaaaaagtat gggtaacact tttggacaat aaatgcaaga agtttttttg
     1321 taagcaagtt tcagtctaaa taatatttga aagaatggct aatctataca aattttaaca
     1381 actatgggta acacttttgg acaagtcaag aaaaaaacaa tttttcttaa tttgttgatc
     1441 gactaaaggt aacacttttg gacaacacag aataaaaagt ccggtgatat cagttttggt
     1501 aggaaacatc ttaatatctt tgtgagattg aaggtaacat ttttggacaa gtcaactatt
     1561 tgttaaaagt taatatagag taattagata agttttttct tttcgtctga taaatggata
     1621 atattagtta ttttattgct aattagtctt tcatgatgac tgaaatagct aaaataaata
     1681 tctcgttgga taattgctga tactttaagg gatatctaaa tttgctgatt gatttattta
     1741 tgttattatg atattataat aacataaatg tgttacgccc gaacctcatt ttaaaaaaat
     1801 gaaggggtga gacaatgaat gctattaatt ggcatgaatt tgttaactat atggagagct
     1861 tactagatga tctaaaaagt tttagtattg ctgatgcagt aaagcatggg ttaccagtta
     1921 gtacaacgat gaaattacga ggattgaaaa ttccagatga aattattaag ctgagaacta
     1981 aaacaattat tgaaaccgca aaagtcgttg acaatatgaa ccatgaacta aaaggcaaga
     2041 aaacaactta cgagaaagat tttgaacgtt ttttaaaatc tgttattgga aaatatctag
     2101 gtaaaaaatc tttcagtgaa ttgagagatg aagagattgt acctactcca atggttagca
     2161 aaatccgaaa aaacaaagcc gaggggaatt actatcttgg tacaatagca aaaatcattg
     2221 attatttgga aaataaagag caaaagaaag gaacgaagaa aggaagataa tttttgtgtt
     2281 aaatattaat ggggaagaat ataccgaaat gattcaaaat cccgaaccta gaattaccga
     2341 aaaaacgtct gattgtttgc tcttttatga aagggggcag acaaactaat ggaaaaaaac
     2401 gtcaattcaa catctaccaa agatcaatac agtattattg gagggttgct tggtgataag
     2461 gataagagaa cacgtcacgg attacgcatt atgcttgcaa cttttctagg agtgcttttt
     2521 ttattaacag gcttgggtag ctacatttat caattaggaa actataatta ttatctcgtg
     2581 aacaatacaa cacctatagg ctcttcattg gagtttgctc gatcaggagc agagatcact
     2641 atttcagatg tgtggacaga tcaaaacaga gatgtaacag tagtaaaact cggttactcg
     2701 caaaacgcta gaaagttgtt atctacgcaa ggaaaaaact acaacttata tatggcaacg
     2761 aaagaaaaag atcgtcctaa aaagcttgaa atgagctatg ggatgttaag tactgatggg
     2821 gatgctttct tattcctaaa aggtaaatta gatgaaagag cttatcaagt ttttattgca
     2881 aaccaagtat cactaatttc tggtgatgat acagctgacg atgataccaa agtcaatgtg
     2941 aatgaagcag attcagttac aaaagcactt tctcaatatt ctatgaatga tgttaataat
     3001 gaaggagtaa tttcttttgg gaaaaaagca aaacctgtta atactcctga taatatcaat
     3061 ttcagaataa atccatattc agattcaact aatgtctatc aaggaagctt tcttaccaaa
     3121 gatggcgata ttgattattc aaaagttgta gcagttacaa gtaagaataa tgttgtaagt
     3181 aagttagaag atcagttaaa tgcttcaaat actaacatca agaaaattga agctacaaaa
     3241 gctgagtatc tggaacgttt acaacaaaat cctgacgata aagattcgca agaaagtatt
     3301 cgtcaactag acagtcgaat ggagaccgaa attaaagcaa tcaatgatac acaaaaattg
     3361 attgatcaat acaaaaatgc tgactttaac aaagattctt ttggagacat gaagacgaca
     3421 tttaagtacc taactctgac tgactagaag taaattattc ttattttaga ggaagtgaac
     3481 acaaacagat ggaaaatagt accgagtttt ttaagaatat tcaaactgaa tttaatcatg
     3541 ctctcacacc aatgaaacca ccgaaacata agttagtggg acgtgatgac gaactccatg
     3601 agttgtcact gatcttgaat agaccggaaa caccgcaagc tatcttgtta ggtgaagccg
     3661 gaacaggtaa aacagcactt gcaagtgaat gggctgaaag acagcgacaa gatggaaaga
     3721 aagtacaagt atacaaatta tcaatcggac aattgcgtgt tgggggagat gatgtcctcc
     3781 aaacacgttt agaaacatta attccaagat tgaaagaata tgagcgaatt ttaaaatctg
     3841 aggacaaaga aaatgaattt gtcctcttca ttgacgagtt tcataaagtt gttagctctt
     3901 ttggtagtgg caataaaatt gggggtgatt cgctaaaaga accgttagca aatgaatcac
     3961 ttcaattgat taccgctact acaagaaaag aatatgaaaa ctatatcaaa agagatgatg
     4021 ccttagggag acggttacaa ccgttagaat tgaatgaact aaaaagcgat gttgtcgtcg
     4081 atattttgag acaatggcta aatacatcct ctgttaaaga tgatcctcag gcttctttta
     4141 aattatcgca aaggattcct gatgaattac ttcaagaaat cgtacaagca aacaagcttt
     4201 atcgtgagga tttgtccgaa ccggctaaat caattgccgt aatggaggat ttgattgcaa
     4261 aagatcgagt agacggaaat gcttataatc gagaaagtct attgcaaata tttggtaagt
     4321 ataatattca gctagattct gattttgata tggaaaaact tcaagaggaa ttaaagtcca
     4381 taaaagggca accgttagca gtttatacta taacaatgtt gtttaaacgt atgaagcttg
     4441 gatttgatcg ttttaaaggt cgaaagcaac caaaagcaac tgctctgttt acaggatcaa
     4501 caggggtcgg taaaactgca atggctaaag ccgtggcaag aggactttat ggcgatgaaa
     4561 ataaagttct cttagttaat atgccggact acaaaacaaa agatgatgca atgcgtttta
     4621 gacgactcat aggtactcat gtaaggcatg atccttctgc gattattgtg tgtgatgaat
     4681 atgagaaagc tggtgtagaa acactcgatg cctttttatc aattttagat gaaggtagga
     4741 taagttttac tgaaagagga tgggatgatc gagaagttca cgacactgtt tcattgtcaa
     4801 atacaatttt atttgcgaca acaaatgcag gtgcggagat gttcgattcg ttaaacagat
     4861 atagctcact atctcgtgtt acaaacaagg aaaattctaa tgaatatagc actgaactaa
     4921 aaagagaata ccgaaaagtc gcatcaggga tcaaacaagc cttgattggt gaaggtctaa
     4981 agcctgaagt tattaaccga tttggacgaa tagttccatt ttgggcttta tccgaacaaa
     5041 ctcttctgga aattgcttat aatgaaatgt tgaagagtgc tgaattagta cgaaaacatt
     5101 taaatataga tttacgtctt ccacctagga aagaaagagt atatggtcaa aatttttatg
     5161 cagacgatct ctccatgcac ttagtttttg acatggcttc acaagaggat tctaaatcag
     5221 ggggtgcaag gaatatcttg catttagttg aatctgagtt tgaagaagcg attactgaag
     5281 caataattac ttatccaaac gcaaaaatat tcgatgttaa gttgagtgaa gccggagcgt
     5341 ttgaaggaaa aggtgattct aatgctgaaa ttattgcaaa gccttccgtt gctgaaagta
     5401 aagaaaacaa gattgtattt tcgattggag gggcgtagct atgaaattca agaaatattt
     5461 actacaaaac cttagaataa aacgatgggt cgtaagaggt ctagttattg gaggagtatt
     5521 agcagttact ttaggcgctc cttttggaat gaatgatcca actcctgaca atcaagatag
     5581 caaacaagaa acagttataa ataagaacgt tgcatatgcg gaaagtaaac cggcagaaca
     5641 aggaatgtgg acactatatg ctcgtatcct aatgggagat aaagaagtta atgatagtgc
     5701 cgctaaaact ggacaaatga atttgttagg acaaggtggt atcagtgccg atttccctta
     5761 ttctgacatt tctaaacaag gtaattcgat tgcagggggt aatgcaggta ctcaactagc
     5821 atctacttta gcaacttata gttactacgg atatattgaa acagtctcag gaaatgctat
     5881 tgccagtcaa gcatcatcca ttctaggaag tattggtagg acgattggta gttttatcgg
     5941 atcaattagt ttaatccttt ctaatattgc tgaatccata aatgaactcc ttggaaaatt
     6001 tttcatgagt atgaatatat tttctcttat ggggtgggga gaaggtggat cggatgtatc
     6061 taatccggta tctaaagcat taactagctt gcttaacaat ttgggattaa ataatgaatt
     6121 atttaaagcg atagcaagct tatctatgct ctttttcaca gcaatactag gttatcaagg
     6181 aatgaaatta ttgtcgaaag atggtgttaa ctcgaaggga ttatcaccag tacttgaaaa
     6241 atggattgtt cgtatttttg tattgtttat attcttacca gtggcaggat cgctattggg
     6301 agtttatatc aacggcatcc agtcagcaaa aaaagatgga gatataccaa caaatgttgt
     6361 ttctgactac atcattaaca caagactttg ggcttctact caaaatcttg ctccatcatc
     6421 tgatactggt ggtgatgtac ctgacggaaa gcctacatcc gaaatcaagt tcgttgatac
     6481 agattatgaa ccatctaaaa aatctgcaat gataagagcg atcaatacta gcagttatgc
     6541 cagacaaaat aatgatctag attctaattc agtcggtatg gctctattaa aagaatggtc
     6601 atcgaatgat actttcaata ttaatactta tgccggtgat gttaatgtcg ccgaaaatga
     6661 tgcttatatg ggaagagcgc ctgctgtttg gacaggctat aatttagggg atggaaaaga
     6721 aggaagtgca attaagaaac caaccaattt aaatggatat atttggtcag caaatcaaaa
     6781 tgtgacagac gacaatcgca agcctgatgg aaaaggaaca gcgcataact ttgatgctac
     6841 tcaaccttgg ggcgttgcta acaatgcaac ttttagcaca cagtcggtag ctttattgct
     6901 ccaatcggca cttgatacat cgggagctaa gttttatgcg tataatatcg ctccttcagg
     6961 aattcaaggc agtgctaaga atatgtcaac agtaaaaaca gaatggcgta cagcatcctt
     7021 agttggtcat ggagctatgg gtaagtttgg ctcgtggcta gcgatgaacg ctgaatcaat
     7081 cgttttagca gttctttact tagcatgtgc ttgggcgatg ttcaatgtga atatcgtgaa
     7141 agcatttaaa cgtgaattta ttcggatttg ggaagtagtt ctaacaggat cacttgcatc
     7201 aggagcaggt gtgatggcaa tttcattagg tgttgttaca acatctctga tcggactaca
     7261 attacctatc tttctgatta cttttattcg tgcatttggt gatactgctg tgggattcat
     7321 tcctaataca ttagttggcg atggcgtagc aaatattgtt cgatctgcta tgattttagg
     7381 tgtaggatat cttttagtct ttaaaaaagg cgaaaaagta aataatttga ttaatatgct
     7441 tattggctta ccacttgaag tagcgtttgg gtttgatgaa cgtgctaagt tattatttgg
     7501 taatagaaat gatgctgtgc gagttgtgga cggcgtttct aaagcaatca agaaaagcca
     7561 aggagctaaa aatccctatt atgatggtgg caacagctca gtaaaagata tggttgcagg
     7621 aggtcttgga gcagttggag cgcaaaaaat ggctgaacgt ttgaacaata tgggagatag
     7681 caatgctgat gctaagtcca ttgctgataa agtaattcct gaaacaccag aaggaatgtc
     7741 attcaacggt ggcggagttg gttccacaat caataatacc tctttcaaag atttagttcg
     7801 tcaagatcaa ctacgtaata actcccaaca atatattgat catttaggac gtatggttga
     7861 tgaaaacgga aactttgtcg atgaaaatgg tgaaatatta aaagatagta aatacggtaa
     7921 agatgctaaa ggcaacttta ttgacgaaaa agggcgtttg gtcgatgaag atggaaacct
     7981 tatagatact aatggtaaac cattggaaga tagtgaagat ggtaatattg gtatggatag
     8041 cgaaggacgt ttaattgatg aaaaaggtcg cttagtcgat gaagatggaa atcttgttga
     8101 cgaaaatgga cgcaggttgc taaatggtga ttttggtcat gatagtaacg gacgtttagt
     8161 tgacgaaaaa ggtcgtttag ttgatgaaga tggaaatctt gttgatgagt ttggtaataa
     8221 acttggtaaa cacagcgatg gatataatgt gaatggagat ttagtcgatg aggatgggaa
     8281 tatcattgag acgagagatg cgcttgtggc agatcgaaat agagaactcg aacaacaacg
     8341 gcatacgcca attgctgttg caattcctga gaatgaagaa gacgagatta ctttagatca
     8401 aactcatcaa gaactgagga atgaaatgag tggtgaggac gtgtcagaca gagtgcatcg
     8461 agaattagga gatggagcat ctggtgaaag tcagtcaaga gagcttaaca gcaactttga
     8521 taatagtgct attcggaatg acacagtact taaagacaga gaatcgctaa caaaagaaac
     8581 ggataaagaa caatcagatg tgaagaaaaa gttcattcct actcctaatg ttaatcaaac
     8641 agataaatat gaaacaaata ttggatcagg aatgtcgaaa gacaatcaat caattagaga
     8701 acctgaactg acaaataaaa ataatagaac aaataattct aaatctactt cagataattc
     8761 agggaaatct aatcaatcaa ccaagaaaac atcaacagct aaatcagccg ttgaatatct
     8821 atcaaaaact ggtaaaaatg tttacacctc agcagataaa gctttagcaa aagctccaaa
     8881 aggtagtcta gcagggataa cctatgaagc taaaaacgaa gctattaaaa taggtaaagg
     8941 aacggctaag gttgccaagg ctactgctac aggagcatcc aaaactggta aagtagtgaa
     9001 gaaatctacc atgtcagtca aggactatat tgtaaaatac ggtaatcaaa aatcaactga
     9061 aaataagtct gaagacgagt aaaaagaagg agcattgatt tgctccttct ttttactttg
     9121 atttctaact gaagatgtca aggaaaattt ctatttatgc aaaaaaatta ttttaaattg
     9181 tctataaatg aattaaatat ttactgttca aagttgtata attaaaaagg gcttatgctg
     9241 gtaaagaaca gaattattat ctaaacaaaa acttgataaa ccagcatttt tagccaataa
     9301 aaaaagtgaa ataatttaat ggaaaatctc ttttaatcat cgaaaattta t
//
LOCUS       DAGCWU010000117         9279 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.117, whole genome shotgun sequence.
ACCESSION   DAGCWU010000117 DAGCWU010000000
VERSION     DAGCWU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.117"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..714
                     /locus_tag="MBZ71_002095"
     CDS             <1..714
                     /locus_tag="MBZ71_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBT4430017.1"
                     /translation="RKSSSNYYEGGNEIVSKMKISEVTLDETDDFTYYKGYKKGWNTL
                     CTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQ
                     LKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKL
                     YSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAK
                     LIIGISSTVTDYISGYLDK"
     gene            781..1275
                     /locus_tag="MBZ71_002096"
     CDS             781..1275
                     /locus_tag="MBZ71_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBT4430018.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1331..1969
                     /locus_tag="MBZ71_002097"
     CDS             1331..1969
                     /locus_tag="MBZ71_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430019.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            2041..2436
                     /locus_tag="MBZ71_002098"
     CDS             2041..2436
                     /locus_tag="MBZ71_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4430020.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2420..4873
                     /locus_tag="MBZ71_002099"
     CDS             2420..4873
                     /locus_tag="MBZ71_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4430021.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4870..6897
                     /locus_tag="MBZ71_002100"
     CDS             4870..6897
                     /locus_tag="MBZ71_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430022.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6894..7916
                     /locus_tag="MBZ71_002101"
     CDS             6894..7916
                     /locus_tag="MBZ71_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBT4430023.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7933..8862
                     /locus_tag="MBZ71_002102"
     CDS             7933..8862
                     /locus_tag="MBZ71_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4430024.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            9107..9223
                     /locus_tag="MBZ71_002103"
     CDS             9107..9223
                     /locus_tag="MBZ71_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBT4430025.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         3151 a         1540 c         1846 g         2742 t
ORIGIN      
        1 agaaagagct cttcaaatta ttatgaagga ggtaacgaga ttgtttctaa aatgaaaata
       61 tcagaggtaa ctttagatga gactgacgat tttacttatt ataaaggata taaaaagggc
      121 tggaatacgc tttgcacata tcttaaaata agagttcctt ttgatgattt agactttttt
      181 gaaagtcata aagatttgat tacccagaca gcaagctcca tatatgataa acaaggtgat
      241 aatgtattag tggatactat tttagtacca cttccagaga attacgaagt aattaacttt
      301 agtcagttga aaattagtga tgtggtttca caagctattg aagacgctga gtcttttatg
      361 agtaatggtg aatatcaacg tgcctttgac agagttcaca ctgcttttca tggatattta
      421 attgaaatac taaaaaaata tgaaattaca gttcctagag acgaaaatct atctaaatta
      481 tatagtagga tacaacaatt aattgaaaaa gaaattcaac ctactgaact tgctgatata
      541 gtgaagacaa cgataaggtc atcaaatgga atgattagct ctcttaatga agcaagaaat
      601 aggcattcac ttgcacaccc taatacaaat attattggaa agagagaagc aaaactaata
      661 ataggtattt caagtacagt tacagattat attagcggat accttgataa gtagcgtcat
      721 taactgattg ctactttttt tatgactatt tttataaaaa ggatgtgata agaattggaa
      781 atgaaagtct atgtggcaaa tttaggacgg tataatgaag gtgaattagt cggagcatgg
      841 tttactccac caattgatga ggaagaaatg gcagaaagaa tcggtttaaa tgaggactac
      901 gaagaatatg caatacatga ttttgagctt ccttttgatg tggacgaata cacgccgatt
      961 agtgaaatca accgtctatg tgaagccatt caagaaatcg aaggtacacc tatttataat
     1021 gaactcaaag aaattcaagg catgtggttt agtagcttgg aagaattact ggaaaacaaa
     1081 gaggatattc attgttatag tgattgtgat tcgatggaag atgtcgcccg ttattacgtg
     1141 gaagaaacag gacagcttgg tgaagtccct tccaatttac aaaattatat tgactaccaa
     1201 tctttgggac gtgatatgga aatagagggt aattacctag ttacctctca tggggtattt
     1261 gaatattgtc agtaagttta acaatccgtg ttataattaa attaataaat agcacggaga
     1321 ggtataaatt atggtaaatg tatttgaaaa tcttaaacta ttaaaactag acatgaaaga
     1381 taaaggctgg gtgatagatt ccttttactt tcgttacaaa cagcaaaatt atattgtact
     1441 tgttaagcta tttgaaaaag aggaaaaagt tccagaatat gctctgttaa aactagaatt
     1501 tttaaaggaa aacgattttt cagatatgct agctgtctat gctaattcgg taaaattata
     1561 tacagacacg aaaacaataa gagaatattt tggtatcgaa tacagtagta acttgggaga
     1621 tgttttattt caatttagcc aaactcttgc tcgatttatt ccgacagaag ttagtgagaa
     1681 gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta agtcaaagtg attcagaaga
     1741 tcccagaaaa aaatattgtt tttcagttag aagaaatcct ttaaagggaa atggtgagct
     1801 gggaaaacga agtcctttta atgacaataa aacgagattg tatagaccca gcttatatga
     1861 acgattaggg tcagatacca atttaagttt cagatattca atgaatccag atgatgaaga
     1921 aactgatgaa ggtattattg caaaatggac aaaaaataaa attgaatagt taaagcattg
     1981 aaagagcaat cacctatttt gattgttctt tttctatttt tacggaaagg acgtgaagac
     2041 atgaaaaaaa taagaagtta caccagtatt tggtcagtag aaaaagtgat ttatgcgatt
     2101 aatgatttac aactcccttt cccaattacc tttacgcaaa tgacatggtt tatcgtttct
     2161 ttgtttgtcg tcatactctt tggcagttta ccaccacttt ctctgattga cggagcacta
     2221 ctcaaatact tagggattcc cattgttctc actgggttca tgtgccaaaa gacctttgac
     2281 ggaaagaaac cctatggatt tctaaagtct atcattacct atgctctacg ccctaaagta
     2341 acctacgctg gcaaacaaat acgattaaaa aaagaaacaa tgaatgaatc gattacgatt
     2401 gtaaggagtg aacaactaga tggcatatcc gattaaatat atggaagata atctcgtatt
     2461 taatcatgat ggcgaagtat tcgcttatta tgaattaatt ccctataatt acagtttttt
     2521 aagttccgat gaaaaaattc aagttcatga taatttctgt caattgattg cccaaaatcg
     2581 tgatggcaag attcatgctc tgcaactagc aaccgaatca agtattcgag ccacacagga
     2641 acaatcgaaa aaagaaatca ctggacgatt aaaagagata gcttacgaaa aaatagatga
     2701 acagaccgaa gcgttaattt ctatgattgg tgacaatcaa gtagactatc gcttttttat
     2761 tggttttaaa ctctacctca atgaacagga agtatccgta aaaaatgttg ggaaagaaat
     2821 tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac aaattaatgg gcgattttgt
     2881 ttctatgcct atggcggaaa tcgaacgctt ttctaagatg gaacagttgt tagaaagtaa
     2941 attatccaga cgctttcaga ttcgtaaatt ggagaaagat gattttggtt atattattga
     3001 acatctttac gggcaaacag gtgtcccttt tgatgactac gactaccatt taccgaaacg
     3061 atacgaagat aaaaaagtgt tggtaaagaa atacgatatt ctcaaaccca ctcgctgttt
     3121 aatcgaagaa aatcaacgct atttacggat tgaacaggaa gataccacta cttatgtggc
     3181 gtattttaca attaatagta ttgttggtga attggatttc cctaatagtg aaatttttta
     3241 ttaccagcaa cagcagttta ctttccccgt tgataccagt atgaatgttg aaattgtcgc
     3301 caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa gaactgaaag atttagatag
     3361 ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg gtggacgctt tagaatctgt
     3421 cgatgaatta gaaagtaatt tagaccaaac caaagaatct atgtacaaac taagctatgt
     3481 ggtacgggta tcagcaaaga atttggacga gctgaaacaa cgttgtgatg aagtaaaaga
     3541 tttttatgat gatctgtcta tcaaattagt acgacctttt ggcgatatgc taggcttgca
     3601 taatgaattt attccctcaa gcaagcgata tatcaatgac tatattcaat atgtcacgtc
     3661 ggattttttg gctggtcttg gtttcggtgc tacacaaatg attggtgaaa attctggtat
     3721 ttacgtcggt tacaatctcg atacaggcag aaatatttat ctccaaccta gccttgcaag
     3781 tcaaggaatt aaaggctctg taacaaatgc attagcgtcc gcttttattg gttcattagg
     3841 tggcggaaaa tcattctcta acaatatgct ggtctattat tcagtactct ttggcggaca
     3901 ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg aaagaaacct tacctgatat
     3961 tgcccatgaa atcaatattg ttaatctcac gagtgagaac gacaataaag ggttacttga
     4021 cccttatgtg attttaaaac tcccgaaaga ttcagaaagc cttgccattg atattctcac
     4081 gttcttaaca ggtatttcca gccgagatag cgagaagttt cctgtcctgc gtaaagctat
     4141 tcggagtgta actcaaagca aacaacgagg gttgttattg gtgattgatg aattgcgaaa
     4201 agaagatacg cccatcagca atagtattgc tgaccatatc gaaagctttg tcgattatga
     4261 ttttgcccat ctgttattca gtgatggcag tgtcacccaa tccattagct tggaaaaaca
     4321 actcaatatt attcaagtgg cagatttggt cttacccgat tccaatagct cttttgaaga
     4381 atacactacc atggaattat taagtgtggc aatgctgatt gtcattagta cctttgcttt
     4441 agactttatc cactctgacc gttccatttt taaaattgtt gaccttgatg aggcttggag
     4501 ttttttacaa gtggcacaag gaaaagctct atctatgcga ttggttcgag caggacgtgc
     4561 catgaatgca ggggtctatt tcgttactca aaacgcggac gatttactag atgaaaagat
     4621 gaagaataat attggcttga aatttgcctt tcgttccact gacctagtgg aaatcaaaaa
     4681 gaccttagaa ttttttggtg tggacaagga agatgaaaac aatcagaaac gcttacgtga
     4741 attagaaaat ggacaatgtt tagtttcaga catctatggt cgtgtagggg ttatgcagtt
     4801 tcacccgatt tttgaagagt tgcttcatgc ctttgatacc agaccgcctg tgcgaaatga
     4861 ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact atagttggat aacgctcctt
     4921 tgtgtgggcg ttattttact tttattaggc acgctgggca atgcagtaca agcgacaggc
     4981 ttagttgatg aaaccattga tacaagcaat gaatactcca agtatggttt gaatcaatat
     5041 cagcttgatt attatgtgga caatagctgg ggctggcttc catggaattg gagtgatggc
     5101 ataggacaat cggtgatgta tggattgtat gccattacta attttatttg gacaatttct
     5161 ctttatctat cgaacgccac aggttatttg gtgaaagaag cctatgcctt agatttcatt
     5221 agtcaaacat cccaagcaat tggcgaaaac atgcagacga ttgcaggcat tacaccgaat
     5281 ggcttttcca gttcagggtt ttatgtcggt ttcctccttc tttttattct cattgttgga
     5341 atctatgttg cctatacagg attggttaag cgagaaacga cgaaagtgat acgagctgtt
     5401 ttaaactttg tcgtggtgtt tattctatcg gcttccttta ttgcgtatgc acccgattat
     5461 attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg ccagtctgaa tattgggaca
     5521 aaaattgttc ttcctaacac ggatagtcaa gggaaagaca gtgtggacat gatacgtgac
     5581 agcttattct ctgttcaagt aaagcaacca tggctattgc ttcaatatgg ctccactgat
     5641 gtagaagcgt taggggaaga acgtgtggaa agtttattgt ctaccagtcc agatacaaac
     5701 aatggtgaag atagagaaaa tgtagtgatt gaagaaattg aagatcatga caatacctat
     5761 ttaaccctac cgaagacgat ttccagatta ggaacagtct tctttttgtt tgtattcaat
     5821 attggtatct cgatttttgt tttccttcta acaggaatca tgattttttc gcaagtgcta
     5881 tttattattt tcgctatgtt tttgccaatc agtttcctat taagtatgtt gccgagtttt
     5941 gatggcatgg gcaggcatgc cattaccaag ttatttaatg tgattatgac aagggctggt
     6001 attaccttga ttattaccgt tgcctttagt atttccacta tgctttattc gctgtcagca
     6061 agttctccct ttttcatgat tatgttttta caaattgtga cctttgcagg gatttatttt
     6121 aaactgggcg atttaatgag cctattcact ttacaaagca acgactctca aaacatgggc
     6181 agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac aaaccagaag atttcaacgc
     6241 agtatgagta agatttttcg tggaaaagca gggtcaacga agcaaaagac aatgacaaca
     6301 aagcagaatc aaaccaataa agctaaccat actcgtcaaa atgaacggac tacaccaaag
     6361 aaagaaacag cactttccaa tgtgaaaaag caacaaggcg gtatcgggca acgattaggt
     6421 gaaaagacag cccatgtatt ggacacgaaa aatcgtctag tcaataaaag aaagcaggca
     6481 aaacaacaga tacaggcgac accaacccat gtcaaatatg cccttcatca aggaaaagag
     6541 aatgtcactc ataatgtaac agactttaaa caatctatgg cagataccaa agctcaacgc
     6601 aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga acattgctca aaagcgtttg
     6661 gagctggata aagcaaaaga aaaaagacga aaagaaaacc catcatctcc tgttcagaat
     6721 aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc ctgttttgtc acaaaacact
     6781 aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa aacaaaagac accaccgaag
     6841 caattcaatc agaggaagct aaataaaaga acggataagc gaggacaaca aacatgaagc
     6901 taaaaataac agtgatttgt agtgtggtct tctctctctt cttttttctc ttattattcc
     6961 tagtcatttt ctttgcagat gatacggaca gtggcgaaaa caacaaggat tcttctattc
     7021 cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca ccgtccgttg attgaaaaat
     7081 atggtaagga atatgggatt gaagactatg tttcttatat cttagccatt atgcaagtgg
     7141 aatcgggcgg tacagcagaa gatgttatgc aaagttccga atctttgggt ttaccgccca
     7201 acagtttgag taccgaagag tccatcaaac aaggggttaa atatttcagt gaattattaa
     7261 ccagtgcaga acaacaagga gtggatatag attctgttat ccaatcttat aattatggcg
     7321 gtggcttttt aaattatgtg aggagtcatg ggaaaaaata tacctatgaa ttagccgaac
     7381 agttttctaa agagaagtcg ggtggtcaaa aagcagacta tcccaatcct atagccatac
     7441 ctgtgaatgg tggttggcga tataactatg gaaatcagtt ttatgttcag ttggtttcac
     7501 aatacctaac cgacacttcc ccaacagaat ttgatgatga aaccgtgcaa gtcattatgg
     7561 acgaagcctt gaaatacgaa gggtttccct atgtctttgg aggggcgtcg cctaccacct
     7621 cttttgattg tagtggctta atacaatggg tgtatgacaa agctggcatt tctctccctc
     7681 gtgtcgccca agatcagtat gacgctacgc aagaaatctc tatggaagaa gcccaagcag
     7741 gcgaccttat cttctttcat tcaacctata atgcaggcac atatgtgact catgtggcga
     7801 tatatttaga gggaaatcgc ttttatcatg caggcgaccc aatcggttat ggcgacttaa
     7861 gcagtcgtta ttggcaagac catctgattg gggctagacg tgttattcat aactaagaaa
     7921 ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa agaaggagaa accaaagaaa
     7981 tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta aagttggcac gcataagaaa
     8041 ttaacctttg ttttatgggt tttattgatt ggtagtgtcg gatttggaat ctataaaaat
     8101 tttactgcca ttgacactca tactgtccgt gaaacagaaa tcattaaaca gcagattgtc
     8161 gataccaacc aagtagaaag ttttgtgaaa tcctttgcga aagactactt ttcgtggcaa
     8221 caatcccaag aagccattga taaacgaaat gaaaaattga cccattattt gacggaagaa
     8281 ttgcaggtat taaatgaaga aatgattcgt aaggatattc ctactagttc tagtgtgaac
     8341 gatatacagg tgtggcaggt ttctcaagtg aatgagaaca cctttgaggt actgttttct
     8401 gttgaacaag ttattaccga agacaaagac aaggaaacta tatcgtctag ctttcatgtg
     8461 gttgtccata tagatgaatc agataatatg gttattatca aaaatccgac aatgagtaag
     8521 aaacctcaaa aatcggacta ccaacccaaa cagctagaaa gtgaccatac agtagataca
     8581 gaaacgatgg acgaaatcac tagcttttta gaaacgttct tccagctcta tcctaccgcc
     8641 acagaaaaag aattgactta ctatgtaagt aatcatgtgt tacctatgat taacaaggaa
     8701 tatgttttcg aggaattggt gaatcctatc tttaccagaa aagataacca agtaatcgtg
     8761 aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa cccaaatctc gcaatttgag
     8821 ttgatattag aaaaacaaga taattggaag attgtaaaat aacaaatatt ggtacatgat
     8881 tacagatact ttgtaatcat gtactctttt tgataaaaaa ttggagattc ctttacaaat
     8941 atgctcttac gtgctattat ttaagtgact atttaaaagg agttaataaa tatgcggcaa
     9001 ggtattctta aataaactgt caatttgata gcgggaacaa ataattagat gtcctttttt
     9061 aggagggctt agttttttgt acccagttta agaatacctt tatcatgtga ttctaaagta
     9121 tccagagaat atctgtatgc tttgtatacc tatggttatg cataaaaatc ccagtgataa
     9181 aagtatttat cactgggatt tttatgccct tttgggtttt tgaatggagg aaaatcacat
     9241 gaaaattatt aatattggag ttttagctca tgttgatgc
//
LOCUS       DAGCWU010000118         8919 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.118, whole genome shotgun sequence.
ACCESSION   DAGCWU010000118 DAGCWU010000000
VERSION     DAGCWU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.118"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="MBZ71_002104"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="MBZ71_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBT4430026.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="MBZ71_002105"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="MBZ71_002105"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBT4430027.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="MBZ71_002106"
     CDS             complement(2169..2906)
                     /locus_tag="MBZ71_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBT4430028.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="MBZ71_002107"
     CDS             complement(2997..3524)
                     /locus_tag="MBZ71_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430029.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="MBZ71_002108"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="MBZ71_002108"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBT4430030.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="MBZ71_002109"
     CDS             complement(4401..4886)
                     /locus_tag="MBZ71_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBT4430031.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="MBZ71_002110"
     CDS             complement(5039..5236)
                     /locus_tag="MBZ71_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430032.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="MBZ71_002111"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="MBZ71_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBT4430033.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="MBZ71_002112"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="MBZ71_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBT4430034.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2520 a         1983 c         1492 g         2924 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt tcgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatg
//
LOCUS       DAGCWU010000119         8873 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.119, whole genome shotgun sequence.
ACCESSION   DAGCWU010000119 DAGCWU010000000
VERSION     DAGCWU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.119"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            23..808
                     /gene="purR"
                     /locus_tag="MBZ71_002113"
     CDS             23..808
                     /gene="purR"
                     /locus_tag="MBZ71_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBT4430035.1"
                     /translation="MTQYLLNHPHELISLTSFAERYESAKSSISEDLGIIKKTFKERG
                     YGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGYVYLSDLLGEPE
                     LLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVRRDSKITEGSTV
                     SVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSLIEEFESELVGI
                     TVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1311..2684
                     /gene="glmU"
                     /locus_tag="MBZ71_002114"
     CDS             1311..2684
                     /gene="glmU"
                     /locus_tag="MBZ71_002114"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBT4430036.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2778..3749
                     /locus_tag="MBZ71_002115"
     CDS             2778..3749
                     /locus_tag="MBZ71_002115"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBT4430037.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3755..3857
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3923..4492
                     /gene="thiT"
                     /locus_tag="MBZ71_002116"
     CDS             3923..4492
                     /gene="thiT"
                     /locus_tag="MBZ71_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBT4430038.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4573..5346)
                     /locus_tag="MBZ71_002117"
     CDS             complement(4573..5346)
                     /locus_tag="MBZ71_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430039.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5613..7313
                     /locus_tag="MBZ71_002118"
     CDS             5613..7313
                     /locus_tag="MBZ71_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBT4430040.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7283..7501
                     /locus_tag="MBZ71_002119"
     CDS             7283..7501
                     /locus_tag="MBZ71_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBT4430041.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7521..8411
                     /locus_tag="MBZ71_002120"
     CDS             7521..8411
                     /locus_tag="MBZ71_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBT4430042.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8429..8713
                     /locus_tag="MBZ71_002121"
     CDS             8429..8713
                     /locus_tag="MBZ71_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430043.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         2986 a         1526 c         1871 g         2490 t
ORIGIN      
        1 tcgcagtgaa cgtctagttg acatgacaca gtatttattg aatcacccgc atgaattgat
       61 ttccttaaca agctttgccg aacgttatga atctgctaaa tcttctatca gtgaagattt
      121 gggaatcatc aagaaaacat ttaaagaacg agggtatgga accttggaaa caatcccggg
      181 tgctgcaggt ggtgtattgt tcatccctga aatttcatat gaagaggcga aaaaatatat
      241 tgaatctcta gcagaacgtc tatcagaaca agaccgtctg cttccaggag gatatgttta
      301 tctctctgat ttgttaggag aacctgaact gctgcgtcaa gttggaagaa tcattgcttc
      361 taaatacctt ggtgaaaaaa tcgatgctgt aatgactgta gccacaaaag gcgtacctat
      421 tgcccaagct gtgtcctatt atctgaatgc gccattcgtt attgtacgcc gggactcaaa
      481 aataacagaa ggatcaacag taagtgtcaa ctatgtgtct ggatcttctg aaagaattga
      541 aaagatggaa ctgtctaaga gaagtttgaa acgtggttca cgtgtccttg tagtggatga
      601 tttcatgaaa ggcggcggaa cagtcaacgg aatgaagagc ttgattgaag aattcgaatc
      661 agaacttgtt ggcatcactg tatttgccga atcaaaattc aacggtcatc gcgccatcga
      721 tgattatacg tcactgcttt atgtcaaaga tgtagatact caaacgaaaa atattacggt
      781 tgtgccagga aattattttt cagaataaga ggttgtgaaa aagctgctta gtttcgagta
      841 gtaagaaaaa ttttgtgaaa atggctttcc acattttttc ataaatttgg cttaataccg
      901 agaaactggc ttttgaacac cgtttatcta gaggttgtga aatcagcgtc ctgtcctgag
      961 tattagctca ataaacggaa aaattgctcc tcatattttg ccgtttattg agctaatacc
     1021 gaaggtcagc tgattgaaaa acgtttaatg aagaggttgt aagcctaccg tttaactcta
     1081 agtcacaaga aacgatttca aaaaacagct tttcatgttt tatgagtagt gtgacttgtg
     1141 accgaggagt tggcaggaga gcaccggtta ccaaatagat aggcccgtta tggtctatct
     1201 atttttttat ccagaaaacg ctgggataaa agaaaatcac ttgttttgtt gaaaaaatgg
     1261 gtatactggg ataaactgaa atcagcaaac tatatttagg agtgacgcag ttggacgcac
     1321 gttatgcaat tattttggcg gcagggaaag gtacccgcat gaagtcaaaa ttatataaag
     1381 ttttacatcc cgtttctggt aaacctatgg tagaacatat cattaatcgg gtcagtgaaa
     1441 cgaaaccaga tgaagtgatt acgatcgttg gacacggagc agaacaagtt aaagcacaac
     1501 taggagaacg cagtaaatat gcgttacaag cagaacaatt gggaacagga catgctgttt
     1561 tgcaagctgc ttctttcttg gaaggaaaaa aaggaacaac gttagttatc agtggcgata
     1621 caccattatt gactactgaa acgctgaaca atctttttga atatcaccaa ggaaaaaatg
     1681 caagtgcaac gatcttgacg gcacaagcag aaaacccaac aggatatgga cgaatcatcc
     1741 gtgaccatat tgggatcgta gaaaaaatcg tggaacaaaa agatgcaaca ccagaagaag
     1801 ctcttgtaca agaaatcaat acaggaactt actgttttga caacgaagct ttgttcgatg
     1861 cgttaagcaa agttggaaca aacaatgctc aaggtgaata ctatctaaca gatatcatcg
     1921 aaatcttaaa agaagaaggc catacagttg ctgcttatca aacggatgat tttgaagaat
     1981 caatgggagt caatgatcgg atcgcgttag ctaaagcaaa tgagatcatg agaaagcgaa
     2041 ttaaccagat gcatatggtc aatggcgtaa gctttgtcga ttcagcaacg acatacatcg
     2101 atgcaggcgt agaaatcggt ccagacacat tgatcgaagc aggtgtacaa atccaaggaa
     2161 atacagtcat tggttctgat tgtgtcatcg ggtcacattc aaaaatcgta gacagtagga
     2221 ttgaggatca cgtcgtcatt gaaaactcag tcattgaaag cagccatgtc aagaaacatg
     2281 cagatgtggg accttatgca catttacgac caaaagctga aattggcgaa aacgtccata
     2341 tcggaaactt tgtggaagtg aaaaatgctc aaatcggcaa aggaactaaa gtcggtcact
     2401 tgacttatgt tggtgatgct acgctgggag aagaaatcaa tgtcggatgt ggtgttgtct
     2461 tcgtcaacta tgatggaaag aacaaacatc atacgactgt cggcgaccac agctttattg
     2521 gttctagcac gaatatcatc ggacctgtcg aggtagctaa aaacagttcg attgctgccg
     2581 ggtcaacaat tacagacaat attccagaat atgcgctggc aattgcgaga gcaagacaag
     2641 tcaataaaga aggatatgca aaaaaactcc cttatctaaa ctaagacttt tgcaaaatca
     2701 cttgatttat ttaaaaaata tgactactat ttattaggta gaattctaga gttgtgaaaa
     2761 agaaagtgga ggtccccatg tcaaaacatt actttgaccc aagattaaaa atttttgcgt
     2821 taaattccaa tcgcccatta gcagagaaaa ttgcagcagc agtcggcgtc gagttaggaa
     2881 aatcatctgt tactcaattt agtgatggag aaattcaagt aaacattgaa gaaagtattc
     2941 gtggatcgca tgtctatgtt attcaatcaa cgagcagccc agtcaatgat aacttgatgg
     3001 agttgctgat catgattgac gcactgaaac gagcaagtgc aaaaacaatc aacgtagtca
     3061 tgccttatta tggctatgcg cgtcaagacc gtaaagcacg tgcacgggaa ccaatcacag
     3121 cgaaattagt agcaaacatg attcaaaaag ctggtgcaac aagaatgctg actttggacc
     3181 ttcatgcagt tcaaatccaa ggtttctttg atattccagt cgatcatttg atgggtgcgc
     3241 cacttattgc caattatttc ttagaaaaag gaatcaaagg cgatgatgtt gtcgttgttt
     3301 caccagacca tggtggtgtc acacgggcac gtaaattagc ggaatttttg aaatctccga
     3361 ttgcgatcat agacaaacgc cgtccaaaag caaatgttgc agaagtaatg aatatcattg
     3421 gacaagtcaa aggcaaaact tgtgtactga tcgatgatat gatcgataca gcaggtacga
     3481 tcacattggc tgccaatgct ttgaaagaag ctggtgcaac tagtgtgtat gcttcatgta
     3541 cacatccagt attatccggc cctgctttac aaagaataga agattcagct atcgagcgtc
     3601 tagttgttac agactcgatc tatcttccag aagatcgtaa aatcgacaaa atcgatgaag
     3661 taagtgttgg cggactaatg ggagatgcaa tcaaacgtat ccatgaaaac aaaccagtta
     3721 gtccattgtt tgaaacaaaa aataaataat aaataaactc acaaagggga gtccaattgg
     3781 gctgagattg aatctatttc taaacccttc gtacctgtat cggttatgcg agcgtaggaa
     3841 ttgtgaataa acagctgttt gactattttc gaaagctgct tcctcctttg tgactttttt
     3901 attcaaagga ggaagttttt ttatgggaaa aaatagagtt tgggtagaag gaacgattgt
     3961 tgcagcgtta gcgatggtgt tgtcgttgat tccgatccag atcggcagca gtttttctat
     4021 ttcattaggg cagattccat tgactctttt tgcgttaaga agagggacaa aggcagggat
     4081 gctggcaggt ttcatttggg gcatcctaca ttttccgttg ggacaagttt attatttaag
     4141 tgtcgttcaa gtgttgattg agtacccgat tgcctatact tttgcaggcg cagcagggct
     4201 catggcttca aatgtccaaa aaggctatgt agacgataat acgaaacaaa ttagaaaaag
     4261 catcgtgttt ggtgcattga ttggcgcact ttccagatat ttttggcatt ttattgcagg
     4321 agtggttttc tggggagcgt atgcactatg ggggatgaat ccttggctct tttctttcgt
     4381 aatgaatggt gccagcgggt tgagtacagc tattgtaacg atcatcgttt tattagggat
     4441 ccaacaaacg actccttcga tttttgtgcc agataaacga caggagctgt aaataaaaaa
     4501 gaagcaagaa acttgagaca gaatcatgtc ctagtttctt gtttcctttt tattttttga
     4561 taagaaatag atctacttca atggcagata agaaagatcg gcatcttcta ccaattgcca
     4621 gtgtcgatcg gtgacagagt agattaaagt agcgatgatt tcctcttctc gataccgggt
     4681 gccttttaat ttccaaatat aatcttcaat cacatagatt gggatacgat atttaccatc
     4741 gatcaaacca acttgatcaa aagaaaaaat gatcccttcc tgcatcaaat tacgcatttc
     4801 tttcaataat tccacactga ttttttcaac aggtgtttct tttttgaaaa aagatttttt
     4861 tacattgatc ttatctgaca agatcgaact taatttacta gcaaaaatcc tataaaattc
     4921 atctaaatag cgataataaa tccgattcag ctgatcatta agatcgaagt atacaaaatt
     4981 attctgtagt ttataaaaaa acggcgaatg aagatgtgtt ttcatatgac caaaatataa
     5041 taattctgaa atttctaaag gtgtcaattc tttcagcatc gttaaatcag taaagtcgat
     5101 ccacttcaat tcatcgaatc cacgcctgtt acgagaagag gaaaagaaac gctgaatatt
     5161 ttctgtacca cggattactt tcagccctgt atgggattca tattctccag cagaagcaga
     5221 tggatcaagc aagagcaagt tttgaggaaa atgcacgata ctgtttgcaa agtcagtagc
     5281 agtcagccct tttgttaaaa cagcattact tgtggtatca atatgtacat aaactgactc
     5341 agtcatcatg ttcctcctct tatttcctct taatctcttt tattttacaa catttgcatt
     5401 aaaaaagctt gtttctttta ttttaaaatt ccattacaaa atcacgtttt cattgaaaac
     5461 actctcattt tcaattaatt tttgtgattt ttgtattttt ttatttaaag gaggttttat
     5521 gaattgtttt cttatggaat ctacaaagtc gaatacgaga ctcatgacct ttttatgacg
     5581 tactggtata cttgaagaaa aaggagagcg cgatggagat aaaaaaagtt tacttttcac
     5641 agaaaatgtc gctgtattcg cctatccagc aagcaaaaca accccttcca ttttttcaaa
     5701 gtcatcaaga aagtagagac gctttttggc gttctgcaaa aaagcaatgt gcttttaaag
     5761 cagtatcgaa aaaatatacc gtctatttta ctattgctaa aagtagagga gatgttattt
     5821 ttaataatct gttgattgaa ggtgaaactt ttgaatggaa aaaatttttt cggtatatgg
     5881 aagcttgcgc tcgttttttt attaaagaaa actgttgttt tttatttcaa tctcctctat
     5941 cagaagaatg gttgcgtatt tttcataaaa acggctatca aggaaccaca caattaaata
     6001 aaaagctggt ctatcataca gcacttgttt taggtggagg aggagcgcac ggtgcttatc
     6061 agattggtgt gtggcaggcg ctgaaggaac atgatatcaa ctttgagatt atcacaggta
     6121 cctcggtagg tgcgttgaac ggcgcattga ttttacaagg agatatgaaa aaagctgtga
     6181 atttatggaa aaagctgtcg acaagacaag tgcttgcgtt accagaaatg gctgcagttg
     6241 aagaccttcg tgaacgcttc tatcgagaaa gacgccaaat gacgaaaaca gcactaattg
     6301 aaggcggggt ttcttctgct ccattggaaa agatggtcaa agacaatttg gatttgtcct
     6361 tactcaaaca taatcccaaa atacgtttat atacggtgtc tactaaactg ccagaactag
     6421 cggaagtagt gactgatatc caacagacaa aaacagaaga aataccagat tggatactcg
     6481 cctctgcttc attttatccc gctatggctt atcgaaagat cggaaaaaat aaatatgcag
     6541 acggtgggta tcgcaacaaa atacctattg atattgcgat taacaaagga gcaacagagg
     6601 catttgtcgt agatgtgcaa gggccaggcc cagcgaaaaa aattcgtatg ccagacattt
     6661 ttatccattg gaaatgtcaa acattgtgga cactaggcag ctttctgctt tttgatagcc
     6721 aaaggaacca attaaattta caattaggct atctggaaat gaaaaaacga ttaggctatt
     6781 actatgggaa ctggtataca tttcattctg ttaaacctgc aagaacatac tggcgaggat
     6841 ttttgtctta cttaacaaaa gaaattcagt tagaattatc ttttttcaaa agcataaaat
     6901 tttggcaaaa actgcgtaat ttatataaga acagggtcgt accagagaca tgcggattgg
     6961 cgatgctaga acttttagca aaaaaacatt ttttactgcc caatgaaatc tatcaagtag
     7021 acatgatgat tcaaatgatc tgccaacaag atataaaaag tatgccggat gatttattgg
     7081 cacaaattgg tcaattaagt actgaagaat ggcgaaggta tcgaaaatac cagcaaaaaa
     7141 tacaaaatga gcgcacgaaa actgtttatt ttgattatct gctacaagga aaacgcaagg
     7201 atcgtttgca gcatgaattg cttaagcaac cagttgacac attactgatc ctatatttat
     7261 attatctcaa ggaggaacaa ccatggcaca agaattttca tatgaaatcg tagaagaaat
     7321 cgctatattg tcagaaaaca acaaggggtg gcgtaaagaa ctgaacctag tcagctggaa
     7381 tggtcgcccg ccaaaattcg atcttcgaga ttgggcgcca gaccatgaaa aaatggggaa
     7441 aggcctgaca ttgacgaacg aagagtttga acaactgcaa aaagcaatag aaaatatgta
     7501 atgaggaagg accctgtcga atgaaaagta tgactggttt tggcaaagcc atccgcgaaa
     7561 caaaagcgta tcagttagat attgaaataa aaagtgtcaa ccagcgtttt ctagatgttc
     7621 aaatacgaag tcctaaaatc ctgaactttg ttgaaaacga ttttcgtcag cttattaaac
     7681 agcagttaag ccgcggtcga atcgaagtat ttgtgaatct agcttatatt ggcaaaaacg
     7741 aaaaacagat cgtcatcaac tgggaattga ttgattcact tatcactcaa ttgacagacg
     7801 gaattcaaca acgatacggt agtcaaaatc aagtgtcaat tagtcggatg gcggaacgct
     7861 tcgcgctgga cgaaaacttc gtaacggtag aagaaaaacc ggaagagagc atggaagaat
     7921 tggcacagtt agctctagat acattgaaaa gcgcacttga ttctattgag gcgagtcgag
     7981 aaaaagaagg tcaagcttta gctttagtgt tgaaaaagaa tactgaagaa ttcaaagaag
     8041 tactaaaaaa actcgatagt tttgtagaaa tctatgaaaa ggaatttcaa ataaggtatc
     8101 aaaaaaagct ggaggaattt cttggagcga aagtcgatga gcaacggttg ttgacggagc
     8161 tagccatctt gttggaacgc ggagatattc acgaggaact ggatcgtctg gcgatccatg
     8221 tacaaaaact agatgaactt atcgaagcac aatgtcctgt aggtcgggaa ttagatttcc
     8281 tgatccaaga aatgaatcgg gaaatcaaca cgatcggttc caaatcaaat gcaatcgaaa
     8341 ttaaaaatca agtcgtccag tctaagacga tcttagaaaa aatcagagaa caagtacaaa
     8401 atattgaatg acgaaagagg tagagataat gaaatattat gacgtaacgt tccacgaatt
     8461 aagtggaaaa actgtggtta aacgggatat cccttctgaa aaaaacggct ttgacgtatg
     8521 gaaagatgcc tgtgcagact ataatgagaa cgaactgttt attttgatca acgacggtgc
     8581 ttacgtgacg atgaatcgga aatttattgt tcgaattgac acagaagagg tagaagatcc
     8641 gacagaaaaa gcacgttctc gaaaagatga gatcatgggt gtagtgaata ctttgtccaa
     8701 tatgggcttt tagcaaggat gctgaacaaa gaaatacaat aaataatcag aattggattt
     8761 cttcgcttag cttctttgct ctaggaatac atttaaaggt tgtgacaaaa ttcgtgtcac
     8821 aatctttttt tattttgaat aagtgatgtt caaaaaccat tttctcggta tta
//
LOCUS       DAGCWU010000120         8853 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.120, whole genome shotgun sequence.
ACCESSION   DAGCWU010000120 DAGCWU010000000
VERSION     DAGCWU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.120"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            441..1550
                     /gene="pdhA"
                     /locus_tag="MBZ71_002122"
     CDS             441..1550
                     /gene="pdhA"
                     /locus_tag="MBZ71_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBT4430044.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1553..2530
                     /locus_tag="MBZ71_002123"
     CDS             1553..2530
                     /locus_tag="MBZ71_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBT4430045.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2555..4198
                     /locus_tag="MBZ71_002124"
     CDS             2555..4198
                     /locus_tag="MBZ71_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBT4430046.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4206..5612
                     /gene="lpdA"
                     /locus_tag="MBZ71_002125"
     CDS             4206..5612
                     /gene="lpdA"
                     /locus_tag="MBZ71_002125"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBT4430047.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5774..6049
                     /locus_tag="MBZ71_002126"
     CDS             5774..6049
                     /locus_tag="MBZ71_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBT4430048.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            6055..6822
                     /locus_tag="MBZ71_002127"
     CDS             6055..6822
                     /locus_tag="MBZ71_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBT4430049.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6990..8819
                     /gene="typA"
                     /locus_tag="MBZ71_002128"
     CDS             6990..8819
                     /gene="typA"
                     /locus_tag="MBZ71_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBT4430050.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT         3012 a         1592 c         1958 g         2291 t
ORIGIN      
        1 aaagaatata acagcttgga tttaaatttc aagctgtttc ttttttttcg gaaaaagaat
       61 acgatataaa gaaactaaga aaaaaccatt atttgtacgt ccgttcacga tgtgtaaata
      121 aaataaatgt ttgcaacaaa actgtataac accaacgttt ttaaatagaa acagatggtt
      181 gtaagtttga ttagaaaata gaaaaaatgc agatgaaata taaaacgctt tccttattgt
      241 gattttattc actaatgctg ttaatatgcg gattggcata atttcctttc atcgtgaaaa
      301 tttgtgcttt tagcttgtat ttctcataaa tccatgataa tttattaatg aagacggtta
      361 aatagtgata cagttttatt cactgtttca atcgcagaat gaagagattt tcttatagat
      421 gagagaggaa tgtgtgacaa atggcaaaca aaaaagcacc gatcgacttc caagcactgc
      481 tagatgaagt caatgcagat ttcccagtat atcaagcact tgatcaagat ggaaacgtag
      541 ttaacaaaga tttagtacct gatttatccg atgatgaact cgttgaatta atgactcata
      601 tggtatggtc tcgtgtatta gaccaacgtt caacagcgct gaaccgccaa ggacgcctag
      661 gcttttttgc accaactgct ggacaagagg caagtcagct ggctagtgct tacgcatttg
      721 ataaagaaga tgttcttctt ccaggttatc gggatgtacc tcaactagta aaacatggtt
      781 tacctttatc tcaagctttt ttatggtctc gaggacatgc tgcaggtaat tcttatccgg
      841 aagaattgaa agctctacca ccacaaatta tcatcggtgc acaatatgta caagcagccg
      901 gtgttgcatt aggtttgaag aaacgaaaca agaaaaatgt cgtcttcact tatactggtg
      961 atggcggttc atcacaaggt gatttttacg aagcaatcaa tttcgctggc gcttaccatg
     1021 caaatgccgt attttatatc caaaacaacg ggtatgcgat ctctacacct cgtgaaaaac
     1081 aaactgcagc aaaaacactt gcacaaaaag ctgtcgcagc tgggattcct ggtattcaag
     1141 tcgatggaat ggatccgctt gcagtatata ctgtatctaa aatggcacgg gaatgggcga
     1201 tcagtggaaa tggaccagtc ctaatcgaaa cactgactta tcgttatgga ccacatacac
     1261 tatctggtga cgatccaacc cgctaccgta caaaagacac agatgacgat tggcagaaaa
     1321 aagatccatt gatccgcttc cgtaaatatt tgacggaaaa aggactttgg tctgaagaaa
     1381 aagaagaaca agtcatcgaa gcaactaaag aagaaatcaa agcagctatt gctgaagccg
     1441 acaaagtacc aaaacaaaag gtttcagatt tcttgaaaaa catgttcgaa gtatctccac
     1501 aatcaatcaa agaacaaatt gcaatttatg aagcgaagga gtcgaaataa tcatggcaca
     1561 aaaaacaatg atccaagcaa tcacagatgc cttagcactt gaactagaaa atgacgagaa
     1621 tgtcgttgtt tttggtgaag atgttgggaa aaacggagga gttttccggg caactgaagg
     1681 attgcaggaa aagttcggag aagaccgagt attcgatact cctttagctg aatctggaat
     1741 cgcaggattg agtttcggtc ttgcgttaga aggatttcgt ccagttcctg aaatccaatt
     1801 ctttggtttt attttcgaag caatggacga agtagtagct caaatggctc gtacaagata
     1861 tcgaatgagc ggcactagaa atctacccat cacaattcgt tcgccatttg gtggcggtgt
     1921 tcatacacca gaattgcatt cggataattt agaaggtttg atcgctcagt ctcctggtat
     1981 tcgagtagtg attccttcca acccttatga tgcaaaagga ctattgattt cttctattag
     2041 aagtaacgat ccggttgttt tcctagaaca catgaaacta tatcgctcat tccgagaaga
     2101 agtgccggat gaagcttacg aagttccttt ggataaagca gcagttactc gtgagggaac
     2161 agatgtatct attatcacat acggagcaat ggtgcgtgaa gcaatcaaag cagcagacaa
     2221 tcttgcaaaa gaaaatatat ctgttgaaat cattgatctt cgtacagttg cgccattaga
     2281 tgtagaaacg atcattcaat ccgtggaaaa aactggtcgc gtagttgttg tccaagaagc
     2341 acaacgacaa gcgggcgttg ccgcgcaagt agtttctgag atctcagaac gcgctatcct
     2401 ttcattagaa gccccaatcg gacgagtttc tgcacctgat acagtcttcc catttggaca
     2461 agcagaaaat gtctggttgc caaatgcaaa agatatcgaa gacaaagtaa aagaaatcgc
     2521 ggaattttaa gaaaagaagg gacgaaacaa agtcatggct tatcaattta aattgccgga
     2581 tatcggtgaa ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac
     2641 gatcaatgaa gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaatccc
     2701 gtcacctgtt acaggaacgg ttaaaaatgt gattgttcca gaaggtaccg tagcaaatgt
     2761 tggggatgta ttagtagaaa tcgatgcacc aggacacgaa gacaatgaag gggacagtgg
     2821 tgtagccgca gaatcccaaa ctccagctaa accggctgct gaacccactg ttgatacaga
     2881 atcagctgga tcgtcaagcg aaggagtctt ccaatttaag ttaccggata tcggtgaagg
     2941 aatcgcagaa ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga
     3001 tgatacatta ctagaagttc aaaatgacaa atcagtagaa gaaattccgt cacctgtcac
     3061 agggacagtt aaaaacgtga ttgttccaga aggcactgta gcaaatgttg gggatgtatt
     3121 agtagaaatc gatgctcctg gacacaatag tgcaccgtca actagtgcgc catcagctga
     3181 agcaccaaaa gaaaaagtgg aaacatcagg ttcagcaagt gtcgttgaag ctgccgatcc
     3241 aaataaacgt gtattggcta tgccatctgt tcgtcaattt gcccgtgaaa aagatgtaga
     3301 tatctcacaa gtaactgcaa ctggaaaagg tggcagagta acaaaagagg acatcgaaaa
     3361 cttcttggca ggtggtccaa gcagtgcacc agcgaaatca gaagcgccag aagcggcagc
     3421 accgaaagaa gcagctcctg cagcagaaag caaaccagct gcaccagcaa aaccattcaa
     3481 gtccaactta ggagacttgg aagaacgtgt agcaatgacg ccaacacgta aagctatcgc
     3541 taaagcaatg gtcaacagta agcatacagc tccacacgtg acattgcatg atgaagtaga
     3601 agtgtctaaa ctatgggata atcggaaacg atttaaagaa gttgctgctg caaatggtac
     3661 gaaacttact ttcttgccat atgtcgtgaa agctttaaca gcgactgtta agaaataccc
     3721 agtattgaat gcatctatcg acgatgcaaa ccaagaaatc gtttataaac attactacaa
     3781 tatcggtatt gcaactgata cggatcacgg actatatgta ccaaatgtga aagatgcaga
     3841 ccgcaaagga atgtttgcta ttgcagacga aatcaatgag aaagcaaaat tagctcatga
     3901 cggcaaacta tctgcagaag atatgcgcaa tggaacaatt acgatcagca atatcggatc
     3961 tgtaggtggc ggttggttta caccagtcat caactatcca gaagtagcta ttttaggtgt
     4021 aggaacgatt gcacaacaac caatcgtaaa tgctgaaggc gaaatcgttg tcggtcgtgt
     4081 gatgaaactg tcattaagtt tcgatcaccg tatcgtagat ggagcaactg cgcaacaagc
     4141 aatgaataat atcaaacgtc ttttagctga tccagagcta ttaatgatgg aaggatgata
     4201 aacaaatggt agttggagat tttgctgtag aattagatac agttgtaatt ggtgcgggac
     4261 ctggtggcta tgtagctgcc atccgcgctg ctgaaatggg acaaaaagta gcaatcattg
     4321 aaagagagta tattggcggt gtttgtttga atgtcggctg tattccttca aaagcactga
     4381 tagctgcagg acaccattat caagagtcac tagattctga aatgtttgga gtaactagtg
     4441 aaaacgtcaa actagatttt gcaaaaacac aagaatggaa agaaaacaaa gttgtccata
     4501 cgttgacttc tggtgttggt ttcctattaa aaaaacataa ggttgaaaca atcgaaggag
     4561 aagcgttctt cgtagatgat cacacactac gcgtcattca tccagattca gctcaaactt
     4621 attcgttcaa taacgcaatc atcgcaacag gttcccgtcc aatcgagatt ccaggattta
     4681 aattcggagg acgtgtgtta gattctactg gcggattagc attgaaagaa gtacctaaga
     4741 aatttgtgat catcggtggc ggtgtgattg gtgccgaatt gggcggagcg tacgcaaact
     4801 taggagcaga agtaacgatt cttgaaggct caccacaaat tttaccaact tacgaaaaag
     4861 atctagtaaa attagtagaa gatgatttca agaaaaaagg cgtaacagtc gtaacgaatg
     4921 caatggcgaa agaagcagtc gataacggtg acagcgtgac agtgaaatat gcagtggacg
     4981 ggaaagaaga atccgttact gctgattatg tgatggtcac tgtaggtcgt cgtccgaata
     5041 cagatgatat gggcttagaa caagcgggtg tcgaagtagg agaacgagga ttgatcacgg
     5101 tggataaaca aggacgtacg aacgtaccta acatctatgc catcggtgat atcgttccag
     5161 gggcggcatt agcacacaaa gccagttatg aagcaaaaat cgctgctgaa gcaatctctg
     5221 gcaaaaaagt agctgtagat tataaagcaa tgccggcagt agccttcact gatcctgaat
     5281 tggcttctgt aggtatgacg atcaaagaag caaaagatgc tggtatcgaa gcaaaagcat
     5341 ataaattccc attttcaggg aacggtcggg cattatcgct tggtaaaaca gaaggattca
     5401 tccgtctagt tacgacgatc gaagataacg tgcttattgg cgcgcaaatc ggtggagtcg
     5461 gcgcaagtga tatggtctct gaattagctt tagcaatcga atcaggaatg aatgctgaag
     5521 atatcgcctt gacgattcat cctcatccat ctcttggcga aatcgtcatg gatgcttcgg
     5581 aattagcatt aggtttacca atccatattt gatttgaata atacaggcga aacgcaactg
     5641 aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt aaacttaaaa
     5701 atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg aatatgaaat
     5761 aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga caacagacga
     5821 aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa
     5881 caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat ccattggtga
     5941 ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat atcgtacagt
     6001 gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag ataaatggaa
     6061 cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat caaatcttct
     6121 caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat
     6181 cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga ggcgaaaata
     6241 ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt ctttatcatc
     6301 gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg tattatgctc
     6361 gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat gagaaatgaa
     6421 cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt gcctagacca
     6481 gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt cggtcataat
     6541 aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta cggatgcgct
     6601 ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc aaaccttagc
     6661 ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt aacaagcggt
     6721 ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg gacttcttgt
     6781 gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat
     6841 tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga aaacatttta
     6901 ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg ccctgtcata
     6961 aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc cgtaacgtcg
     7021 caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta ttaaaacaat
     7081 cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca aatgcgctag
     7141 aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat aaaggtattc
     7201 gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta gaacggatca
     7261 tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa
     7321 cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt gtcgtaaaca
     7381 agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt gaactattca
     7441 tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca tctgcactaa
     7501 atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg gcacctattt
     7561 ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa ccattacaat
     7621 tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat
     7681 ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac ggaacagtta
     7741 agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta gagattcaag
     7801 aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt gtcggtgaaa
     7861 cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac gaaccaacat
     7921 tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt aaattcgtaa
     7981 ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta tccttacgag
     8041 ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt
     8101 ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct cgtccagaag
     8161 ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata
     8221 caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc
     8281 aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca cctgcccgcg
     8341 gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga atcatgaacc
     8401 atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt caccaagggg
     8461 cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt atcgaagaac
     8521 gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt atcggggaaa
     8581 acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac
     8641 ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg acgttggaag
     8701 aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa agcattcgct
     8761 tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat
     8821 ctgtaaaagg atgaagagag aaaccagctt gtt
//
LOCUS       DAGCWU010000121         8739 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.121, whole genome shotgun sequence.
ACCESSION   DAGCWU010000121 DAGCWU010000000
VERSION     DAGCWU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.121"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            137..1540
                     /locus_tag="MBZ71_002129"
     CDS             137..1540
                     /locus_tag="MBZ71_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBT4430051.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            1540..2973
                     /locus_tag="MBZ71_002130"
     CDS             1540..2973
                     /locus_tag="MBZ71_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBT4430052.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            3118..4044
                     /gene="trxB"
                     /locus_tag="MBZ71_002131"
     CDS             3118..4044
                     /gene="trxB"
                     /locus_tag="MBZ71_002131"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBT4430053.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYGSVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            4258..5985
                     /locus_tag="MBZ71_002132"
     CDS             4258..5985
                     /locus_tag="MBZ71_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBT4430054.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            6000..6350
                     /locus_tag="MBZ71_002133"
     CDS             6000..6350
                     /locus_tag="MBZ71_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBT4430055.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(6442..7302)
                     /locus_tag="MBZ71_002134"
     CDS             complement(6442..7302)
                     /locus_tag="MBZ71_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBT4430056.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRVFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            7394..7822
                     /locus_tag="MBZ71_002135"
     CDS             7394..7822
                     /locus_tag="MBZ71_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBT4430057.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            7984..8160
                     /locus_tag="MBZ71_002136"
     CDS             7984..8160
                     /locus_tag="MBZ71_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBT4430058.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            8187..8633
                     /locus_tag="MBZ71_002137"
     CDS             8187..8633
                     /locus_tag="MBZ71_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBT4430059.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         2932 a         1479 c         1953 g         2375 t
ORIGIN      
        1 ttttgacaag atatttacaa aaatccacta gaaaacagta aattttgtaa atgttactgg
       61 aaagaaacag atttctattg tttgttttgt ttacaaaaaa gataatgaaa gtgtaaaaaa
      121 gtaaaggagt gagaacatgg caaaaggaat caaaatcgct acgattggtg gaggcagcag
      181 ttacaccccc gagttgatgg aaggatttat caaaagatat gaggaattac cgatacgaga
      241 aatctggctc gtagatgtag aagaaggaaa agaaaagctt gagatcgtta gtcagatgtc
      301 tcagcgtatg tgggatgctt ctccgtatga agtcaagatc tatgcaactc tggatcggga
      361 agaagcattg aaagatgcgg attttgttac gactcagttt cgtgtcggtc aattagatgc
      421 acgaatcaaa gacgagcgga tccctagtta ttatgggatg attgggcaag aaacaaatgg
      481 tgctggcggg atattcaaag cttttcgtac cgttccagtc gttttggcaa tcgttgagga
      541 catgaagcgt ttatgtccaa atgcttggct gatcaatttc gcgaacccaa gtgggatggt
      601 gacagaagca gttatcagat atggcaaatg ggataaagtg attggattat gcaatgtgcc
      661 agtgatggcg atgatgacag aaccagcaat gcttggcaag aaaaaagaag agctgatcta
      721 caagtttgcc ggtctaaatc atttccattg gcacaaagta gcagataata aaggaaatga
      781 ccgcacggca gaattgattg atcgtttata cgatgaagag aatggattac cgaaaaatat
      841 ctttgatatc ccgtttttca aagaacaatt aaaacaaatg aacatgatcc cttgtggcta
      901 ccaccgctat tactatcgtg aagaagaaat gctggaacat gctttagaag aatatcaaac
      961 gattggtaca agagcgcagc aagtgaaaga aacagaagca gagctatttg aattatacaa
     1021 agatccagaa ttagaccata aacctgaaca attgcaaaaa cgaggtggcg cttattattc
     1081 agacgctgcc tgtgaaacga ttgcttcaat ctatggaaat aaagaaacac agatcgtggt
     1141 atctacaaag aatgatggag ctgttcctga cctgccggca gattgtgtag tagaagtcac
     1201 tgcctatgtt ggcgcacaag gagcgagaaa tgtagcgttt ggttctcttc ctgctgctga
     1261 aagaggctgg ttgcaagtaa tgaagaatat ggaattgctg acgatcgaag cagctgtgac
     1321 tggtgattat gggacagctt tacaggcgtt tacgatcaat ccacttatac gatcaggaaa
     1381 gacagcaaaa cggatcatgg atgagttgtt tattgcacac cagcagtatt taccgcaatt
     1441 caaggagaca atcagccgat taaaagaaga aggaatcgaa gttcaagatg aactggcaag
     1501 agaattatca ggtaatacac atataatgga ggaagtataa tgggattcag aaaagatttt
     1561 ttatggggcg gtgctacagc agccaatcaa tgtgagggcg gttatgatga aggtggacgc
     1621 ggactagcaa acgtagatgt tgtgccaatc ggaaaagacc gttttcctgt tatcaccgga
     1681 aagaaaaaaa tgtttgaatt tgatgaggat catttttatc cagcaaaaat tgcaatcgat
     1741 atgtatcatc gttataaaga agatattgct ttgtttgcag aaatggggtt caagacttat
     1801 cgaatgtcca tcgcctggag tcgtattttt ccaatggggg atgagaaaga gccgaatgaa
     1861 gctggcttga aattttatga ggatgccttt aaagaatgcc aaaagcacgg aattgagcca
     1921 cttgtgacga ttacgcattt tgattgtcca atgcatttga tcaaagaata tggtgcgtgg
     1981 cgttccagag aactcgttgg tttttatgaa aatctgtgtc gagtcatctt caatcgctat
     2041 aaaggacttg tcaaatattg gctgacattc aatgagatca atatgatcct tcatgcacca
     2101 ttcatgggcg cagggctgta ttttgaaaaa ggagaaaatg aagaacaaat caaatatcaa
     2161 gcggcacatc atgagttggt tgcaagtgcc atcgcaacga agattgctca tgaagtagat
     2221 ccggagaaca aagtaggctg tatgctggca gcaggagcat attatccata cacttgcaat
     2281 cctgaagatg tttgggcttc tagaaaatca gatcgagaaa attatttctt cattgatgtc
     2341 cagtcacgag gagaatatcc cgcttatgca ttgaaagaaa tggagcgaaa tggcattcag
     2401 atcgaaatgg aaccagggga tgccgcattg ctgaaagaac atactgttga ttttatctct
     2461 ttttcttatt atgcttcccg agtttcgact accgacaacg aactcttgga acagactgca
     2521 ggaaatatct ttgcttctgt caaaaatcca tatctgcaag caagtgaatg ggggtggcaa
     2581 attgatccat taggcttgag aataacgatg aatgatattt atgatcgtta tcaaaaacca
     2641 ttgtttattg tagaaaacgg cttaggtgca gtcgatacgc cagatgaaaa tggttatgtg
     2701 gaagatgatt accgaatcga ttacctggca gcgcatatcc aggcgatgaa ggatgcagta
     2761 gaactagacg gagtcgatct attaggctat acgacttggg gatgtattga tttagtatca
     2821 gctggtaccg gtgagatgaa gaaaaggtac ggattcattt atgtcgatcg tgataaccaa
     2881 ggaaatggca cattagcccg ttcaaagaaa aaatcattca actggtataa aaaagtaatt
     2941 gattcaaatg gagaaaatct ttcgaacgat tgatcaatga tttataaaag tctattgttt
     3001 ttcagcggat ttttcgagta taataaagct attcgagaaa cgcaagcaga acaatagact
     3061 ttttttcgca ttggctgtaa agtatgctta cttgattaag taaaggagaa tctacatatg
     3121 tatgatgtaa tcgttattgg cgcaggtcct gctgggatga ctgctgcttt atatgcttca
     3181 cgttcaaact tatccgtatt gatgatcgaa caaggtgccc ctggcggcca aatgaacaat
     3241 acggcagaaa tcgaaaatta tcctggattc gattcgatca tgggccccga tctagcttat
     3301 aagatgtatg gaagtgtctc aagatttggg acagaaaatg cttatggaat cgttcaagat
     3361 atcaaggatc acggcagcca taaagaagtc atctgtgaag ataaaagtta cgaaggaaaa
     3421 acagtcatta tcgcaacagg atgtatccac cgtaaattag gcgtacctgg cgaggaagaa
     3481 tttgctggac gaggcgtttc ttactgtgca gtctgtgatg gtgcgttttt ccgtaacaaa
     3541 cgtctagtag tcatcggggg tggggactca gcagtagaag aagccatcta tttgacacaa
     3601 tttgcttctg aagtagtgat cgtccaccgt agagatgaat tacgtgcaca aaagatcatc
     3661 caagaccgtg catttgcaaa tgaaaaaatt tcctttatgt gggatacagt cgttgacgaa
     3721 atcgttggaa acgaaatggt agtgacaggt gtgaaagcac gtaatgtcaa aaccggcaat
     3781 gaatttgatt tagcagcaga cggagtattc atctatgttg gactggatcc attaactgca
     3841 ccttttagag aagcaggttt gacgaacgaa gctggctgga ttccaacaga caacgaaatg
     3901 aaaacgaata tgccaggcgt ttttgccatc ggagatgttc gtgaaaaaga tttgcgacaa
     3961 atcactacag ctgttggtga cggcggtatt gctggacaac aagtgtataa atacttggag
     4021 tctttagaag aaacaacagc ataagtttaa aaagagtaga agaacgggtt caattgttct
     4081 tttactcttt tttttcgcgt gaaatttact gaatctgatt tatgcgaaca cgtccaacac
     4141 cttttgataa aagaatcctt tcttgattta agcgtttttt ggtcctcata ctttcatatc
     4201 taataagtga atgctatact tattaagaac catatttcag aaagaggtgt tattttcatg
     4261 tcttgggaac aagtgtatga acaatgggta catgaagaaa atttagaaga aaacttaaaa
     4321 aaacaattaa atgatctggg tgaagatcct gaaaaacttg aagatgcttt ttatgcaccg
     4381 ttggaattcg gtactgcagg tatgcgggga atcttaggac ctgggatcaa tcgaatgaac
     4441 atctatacgg taagacaagc aacagaagga ctagcacgtt tcatggatac acaaggtgaa
     4501 gaaacaaaac gccgaggtgt cgctatcgct tatgactcac gccatcagtc accagaattt
     4561 gcaatggaag cagccaaaac actagcacat cacaatatcc cgtcttatgt attcgaaagt
     4621 ctgcgtccaa caccagagct ttcttttgca gttcgtcatt tgaagacgtt tgcgggaatc
     4681 atggtaaccg catctcacaa cccagcggct tacaatggat ataaagtcta cggagaagat
     4741 ggcggacaaa tgcctcctgc agacgcagat gcattgacga aatatgtccg tgaagtcagc
     4801 aatccgctaa aagtcgaagt attatctgac gaagaagcta aacatagcgg gttgatcacg
     4861 atcatcggag aagaagtgga cgctgcttat ctagaagaaa tcaaagcagt gacgattgat
     4921 cgtgaattgg ttgaaacaat ggggaaagat ttgaaacttg tgtacacgcc acttcatgga
     4981 acagggaaaa tgttgggcga acgtgcgttg aaacaagctg gattcgaaca attcgtacta
     5041 gttcccgaac aagctgtggc agatccagat ttcactacgg tcaaatcgcc aaatccagaa
     5101 gaacattcag cttttgaata tgctatacga cttggagaaa aagaaggagc tgacttgctg
     5161 attgcaacag atcctgatgc agaccgttta ggcgcagcgg ttcgtttgcc agatggttct
     5221 tatgaagtat tgactgggaa ccaaatcgga gcattgtttg ctcaatatat tttggaagca
     5281 catcaatcaa gaggcacttt accagaaaac gcagcggtat tgaaatcaat cgtttccagc
     5341 gaattgccta cagcaattgc ggaacactac ggtgctacca tgttcaatgt gttgacaggg
     5401 tttaaattca ttgctgaaaa aatccaacaa tttgaagaag accattcaca tacttttatg
     5461 tttggattcg aagaaagtta tggttattta gttaaacctt ttgttcgcga taaagacgcg
     5521 atccaagcat tgcttttatt agcagaagta gcagcttact ataaaaaaca aggcaaaacg
     5581 ttgtatgacg gcttgcaaac gatttttgaa acctatggct atttcgcaga aaaaacgatt
     5641 tctgtgacaa tgagcggaca agaaggttct gcgaaaatct ctgctttgat ggaaaaattc
     5701 cgtaaagaag caccgacaga atttgccgga gcaaaagtta ttcaaacaga agactttaaa
     5761 gaattgacga gaacagaccg tgaaggaaac gtagaaaaac taacaacacc gccatccgac
     5821 gtattgaagt atacattgga agaaaatggc tggatcgcta tccgcccttc aggaactgag
     5881 ccgaaaatca agttttatat tggtgtaaaa ggaagctcta cagaagacgc agccgaaaaa
     5941 gtagcctctt atgaagcgat tatcaaaaag atgacagaag aataaaaaac ttgagactga
     6001 tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa gttggaggtg aatggattgc
     6061 cagacacgca aaaatcaaaa gtagagaaag tcagtcaatt attcaaaatg ttgagtgatc
     6121 ccactcgtct taaaattctg ctatatctga aagacgggga acaaaatgtg acagcaatca
     6181 cccaagcagt ggaaatggag cagtctgctg tatcgcatca actacgttta ttaagagaaa
     6241 atcatgtcgt caagtctcat cgcgagggaa aagcgatttt gtatagtctt gatgattctc
     6301 atgtactgga tattttgaac cagacactca aacatgtgga acatcaataa cgataggtga
     6361 aaatagataa acgctggagt gtgacaacga agaaatttcg ttgtcacact ccagtgtttt
     6421 ttaagaaaaa gacaaaaatg attaatcgct tttttctgtt gtttcagttc cataataact
     6481 atgatcttct aggtctaaag cattcaaaat aagcgaagcc gtttcttcaa tagataaaga
     6541 tgccacattg atcactacac atcctagttt ttcataaaga tcattcgcaa aatcgagttc
     6601 agctctaatt ttttcgatat cggaataagc agtatcaggg tttaatccgt aagagcgcat
     6661 ccgttcttta cggatattat ttaaaatatc tgggttattc gttagtccca cgattttttt
     6721 aggatcgatt tcccacagct gtttgggaat atgtgcttgg ggaatcaaag gtaaattagc
     6781 aacttttaaa tttttatttg ctaaaaacaa gctcaaaggc gttttggaag ttcgggatac
     6841 gcctagcaaa acgacatctg cttctaaaaa aacacgagga tctttcccat catcgtattt
     6901 tacagcaaat tccattgctt tgatccgttt gaaatagttc tcattcaata aatggagggc
     6961 accaggttgc cgaattggct tgataccagt taaacgttca atttctgata tcggtggcgt
     7021 catcacatcg aaactaaata attgatgttc ttcacaaaaa gaacgtgtta tctcgatcag
     7081 ttcttgattg attaatgtat gcaggacaag tgctttttct tctaatgcat cttgcaatgc
     7141 ctgccttaat gttttttcat ctgtcacgaa cgttctgcgg aataggttga actcaacggt
     7201 aggatactga gccatagtag cttgcgctag ttttgtcgca gtttctccag cagagtcgga
     7261 gatgacaaaa aaagtaacga tttctgtaga atcggaagac atggataaaa cctcacttgc
     7321 atttttttta tgtttattat accatactct agaatgcgta cttcgtaatc attttgattt
     7381 ggaggaataa gtcatgaaaa aacagacatt gatcgaacaa tcattagaaa cattgaaaaa
     7441 taacggtttt aaatatacta aaaaaagaga agcattgtta acttatttag tgaagcgtaa
     7501 ccgctacgta tctgctaaag aagtttttga ctatatgaat caggaattca aaggggtaag
     7561 ttatgatacg atttaccgga atctccatga ttttgcagag atcgatttac tggaagaaac
     7621 cgatttaaat ggtgaaatga agtttcgttt tcattgctgt cagggggaaa tcggccatca
     7681 ccatcatttt atctgtacag tgtgcggaac aacgaaagaa atccatatgt gtccaatgaa
     7741 ttatttcgaa gaacagctag gtggatgcac gatcgaaggg catagattcg agattttcgg
     7801 aagatgtgaa aattgtcaat aaggatttaa ataaaataac atttgataat ttaaaaaaaa
     7861 agcaagattt ccttgacggt caaaacagca ttagatataa tatttacagg acagtcttgt
     7921 tttttgagat atatttcaat aacaattacg gttacttttt aagctcggag ggagggaatt
     7981 aacatgtcaa aaacagtcgt tcgcaaaaac gaatcacttg acgatgctct tcgtcgcttc
     8041 aaacgttccg tgtcaaaagc gggtacttta caagaatctc gtaaacgtga attctacgaa
     8101 aaaccaagtg taagacgcaa gaaaaaatct gaagcagcta gaaaacgcaa aaaattctag
     8161 ttctgtgatg aattggagtt ggtagtgtgt cactactaag tagattaaac gatgacatga
     8221 aagcagcgat gaaagcgaaa gataaggaat ctttacaagt catccgaatg atcaagtcat
     8281 ctatacaaaa cgagcagatt aaagaaggtc acgacttgac agaagaagaa gaactaacgg
     8341 tgttgtctcg cgaaatgaaa cagcgacgtg attctttgca tgagtttgaa gaagcaggac
     8401 gtgacgattt agctgaaaaa gtaaaaagtg aaattgtaat agtcgaaaaa tatatgccgg
     8461 aacaactttc cgacgaagaa attcgacaac ttgttcaaga agcaatcact caaactggtg
     8521 cttcatcaat gaaagaattt ggcaaagtta tgggtgcaat tatgcctaaa gtcaaaggaa
     8581 aagcagatgg aaaccaagtg aatgcgattg tgaaagaact attacaatct taattttatc
     8641 atttaaactg cggacagtga ttgtccgcag ttttttgtgt tcgtaaaaaa agaactaaaa
     8701 aacagctaga agaaagaata agattctttc ttctagctg
//
LOCUS       DAGCWU010000122         8682 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.122, whole genome shotgun sequence.
ACCESSION   DAGCWU010000122 DAGCWU010000000
VERSION     DAGCWU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.122"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..218
                     /locus_tag="MBZ71_002138"
     CDS             <1..218
                     /locus_tag="MBZ71_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430060.1"
                     /translation="KALPDYTGKKAKEDIQSVLKEYERQGILFVSAAIGQDKEEIKSI
                     YGESRFVDITDLNEFPKQMIQLIARYL"
     gene            347..1990
                     /locus_tag="MBZ71_002139"
     CDS             347..1990
                     /locus_tag="MBZ71_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBT4430061.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGNGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPEF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(2063..2608)
                     /locus_tag="MBZ71_002140"
     CDS             complement(2063..2608)
                     /locus_tag="MBZ71_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBT4430062.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEARLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(2631..3116)
                     /locus_tag="MBZ71_002141"
     CDS             complement(2631..3116)
                     /locus_tag="MBZ71_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4430063.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(3106..3918)
                     /locus_tag="MBZ71_002142"
     CDS             complement(3106..3918)
                     /locus_tag="MBZ71_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBT4430064.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(4057..5589)
                     /locus_tag="MBZ71_002143"
     CDS             complement(4057..5589)
                     /locus_tag="MBZ71_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBT4430065.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRACQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPDLSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEVHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQNAVFLQVYGQSECGPMILKAHTLESLKISD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(5698..6729)
                     /locus_tag="MBZ71_002144"
     CDS             complement(5698..6729)
                     /locus_tag="MBZ71_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4430066.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFRTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFELTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVQVSSI"
     gene            7462..8448
                     /locus_tag="MBZ71_002145"
     CDS             7462..8448
                     /locus_tag="MBZ71_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBT4430067.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDTLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDENLEDDTITIPIKEEQ
                     VDVSKHTVVREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHMEDRNKKS"
BASE COUNT         2855 a         1582 c         1648 g         2597 t
ORIGIN      
        1 caaaagcgtt gccggattat acaggcaaaa aagcaaaaga agatatccag tctgttttga
       61 aagaatatga gcgtcaagga atcctatttg tctccgcagc tattggccaa gacaaagaag
      121 aaatcaagtc gatctacggt gaaagtcgtt ttgtagatat tactgacttg aatgaatttc
      181 ccaagcaaat gattcaactg attgcccgct atctatgata aaataaagct caacgaacgc
      241 acttagcaga ttcattgagc ttttcgtttc tagttaccgg taacaaggat acttctaaaa
      301 tagaatcggt tataatcaag agggaccgtt aaaggagtga aacgatatga tacaagaaca
      361 aaaatggtgg aaagaagcag taggctatca aatctatcct gctagtttca aagatagcaa
      421 cggcaatggt caaggagaca tcaatgggat ccgtgaaaaa cttaattatt tgaaagatct
      481 tggtatcggc tttatctgga tcaatccgat ctatcaatct ccatttgtcg ataatggtta
      541 tgacgtaagt gactatcaag atatagaaga gagattcgga acgatggaag attttgattt
      601 attgctaaaa gaagcacatg aattagggat caaagtgatt atggatcttg tcatcaatca
      661 tagttcggat cagcatcaat ggtttgaaga atcgaaaaaa tctaaagaaa gcccgtatcg
      721 cgattattac atttgggtcg acggggtaga tggcaaagaa ccgaataatt ggacatcgat
      781 tttcggcggt tctgcttggg agtattctca tgaaactgga cagtattatc tgcatgtttt
      841 tgcaaaagaa caaccagact taaattggga aagcgagaaa ttgaaagaag aactattcaa
      901 tatgatccga tggtggttgg ataaagggat agatggattt cgtttagatg cgatttctca
      961 tgtaaaaaaa gatgaatatt cagtaaaagc cacagagaac cctttctcac cctttcaaaa
     1021 tgtctcagga atcgaagaac atttgaccga tttgaaacat gtttttgaag agtacgatat
     1081 catgactgtc ggagaagcaa gtggtgtcac tgcagaagaa ggaccacaat gggtagggaa
     1141 agacggttat ttcgatatga tattcgaatt tgatcatatc catatctggc agcaagaaaa
     1201 agaagggcaa ttagatgtcc ttaaattgaa acatgcatta agcgcttggc aaacttcatt
     1261 ggatggtatc ggatggaacg ctttatatat ggaaaaccat gatgttcctc gtgccgtttc
     1321 agtgtttgga gatactcgtc ccgagttttg ggctatgtct gcaaaagcaa tcgctatgat
     1381 gtatttcttt ttgcaaggta cgccatttat ttatcaagga caggaaatcg gaatgacgaa
     1441 tatgcccttt gaatcgatcg atcaagttga tgcagtagat tcgaaaagac tttataaaag
     1501 gctgcttgcg gaaggtaaga caagagaaga agcattggat atcattcgtg aaacaacgag
     1561 agacaacagc aggacaccca tgcaatggac ttcggaacaa tatgctggtt tttctacaca
     1621 tgaaccttgg ctgatcacta atccaaatac caaaacgatc aatgtagaac aacaagaata
     1681 tgaaccagag tcggtcctgc aattctataa gaacatgatc cgcattagac aaaccaataa
     1741 aggtctgata tacggttctt ataaagaata tcttcatgaa catccacaac tttatgtcta
     1801 tgaaagatat ttagaagatg aacaatatct tattatggta aacttaacag aatcacttgc
     1861 tgactatgaa cttccaaaag aagcagatca gtcctggaca ttactcttgt caaattcctc
     1921 tagcggagaa tttgaagcta aaggcatact agcaccttat gaagctcggt tatataaaac
     1981 aaacaagtaa aacttcctcg ttaaagttca ttagttggac tttgcgagga agtttttcag
     2041 attgctctgt caattactca tttcaataat tcgctctagg accaccgata tatttaggac
     2101 gagagcgaag ggctgtcacg tgagcttgtc caagggtttt caaacgaggt ctgacaggta
     2161 cttggacatg tttgatatgc ataccgattg atgtgtcacc gatatcgata ccggcttgtc
     2221 caactacgtg ttccacttcg acaggatcat tgaactggtc aaaagcagca acagaacacg
     2281 cacctccagc atgtagtgca ggaacaacag agacaatttc gaaatttttt gcttctgcca
     2341 ctgttcgttc tactactaat gctcggttca aatgttcaca accttggaca gctagcgcaa
     2401 tttctttagg atccaacaac tcttttaacg tacggatgat ccattgacca acttccgcac
     2461 tggaattttt tccaatatgt cctccaacga cttcgcttgt gctgcatcct aaaacaaaaa
     2521 gatcaccttt tttcaagcgt gcctcagcca atatgtcatt aactatttca gtcaattggt
     2581 cttttaattc tttttctgac aacttcattt tctaaaacct tctttttcaa ttaatattgc
     2641 ttccgtgctt gaatacgttg aagacttgca gttataggaa aggcaagtcc cattccaatc
     2701 agattttgga agatgtttcc tggtatagaa gccactccag ccggccaatc atataaaatc
     2761 caaccggcga taccatagcc gatgatcata actaagcttc ctaataaaag ccctaatccc
     2821 cacatacgag tagaagattt gctgaaagaa ccagcaacat atccttgtaa cccgtggatc
     2881 actaatgaaa aaactgccca ctgcggatat cctgaaatca aatcgatcaa tagaccactg
     2941 gaagcaccaa caactaatcc acccgttgga ccataaagaa aagcagcggt atagatcccg
     3001 gcctcgcata atgtcacaaa tccattcgta gccggtacag ggatcaaaat tgttaatgaa
     3061 agggcaactg tcaacgcaat cagcatagca tagatcgtta attgcttagt tttcatttct
     3121 cattcctcct ttttgaatac cgccagacca gacgcttccg gtatgatcgt tcatcagcac
     3181 accattagaa atacaatggg caacaaattt tttactttct gctactgctt tggtaaagtc
     3241 tgcccctaac acaagattcg ctgttattgc agaagaaaag gcacaaccgg ccccgtttac
     3301 tgttgtagtg tcgatttttt tatgagaaaa tacctcataa tgatcaattg aatagagaag
     3361 atccacagct tcatctccag ccatccttgt gccgcctttg atcacaatgt tttttgttcc
     3421 tgaagcagtg agttctttag cgagatggat catttctgtt aacgaagagg gaagagcttg
     3481 accagaaagt aaagcagctt ctgccaaatt aggcgtaatc atcgtaacaa gtggaaaaag
     3541 ttcttcgatg agctgcgaaa tatacaattg attgatcaca gcttcagttt ctttgaatgc
     3601 caaaacaggg tctaaaatga ttgggatatt ttgttttgtt tttatgaagt ctcgaacaac
     3661 catgattgct tcacttgagt tcaataaccc aattttgata ccatctaatg aagtcatttt
     3721 atcaatcgaa actaactgtc gtttaagcaa ttccaaagat atagattcca gcataaatcg
     3781 ctcattaata tctagtgccc ctatagaagt caaggcgctt agtccgaata gaccaaaatt
     3841 ctcaaaagtt tttaaatctg tttggattcc tccaccagca aatggatcag aaccgccaat
     3901 tgtcaagatt cttttcataa cattttcctc catgaataag agtataacga aattttcaaa
     3961 aaagaaaaac agccaattgg catattttta tctcatccaa tttaaaaaaa caaaaagaaa
     4021 agaaagacca aaatggatct ttctttcctt aaatagttag gacttttgtg attttaattc
     4081 tttctcgatt tgaagccgtt gcactttcat tgtagctgtt cgcgggattg catcaaaatc
     4141 acggatgata gggacgttca agcgaggaag ttcatggact tgctcccacc atcgattcca
     4201 atccatttct ttgtcaggag ctaaagcaat aatcggttgt ggagaatgat ttttatccct
     4261 tacaatcact acttctgcca aaaaatctaa cgaatctaat aaaaagtctt caatagctaa
     4321 tgtactattg atcgtttcga tcaaatccac ttgccgatct ttcaaaaata ggtggccatg
     4381 cttatccata aaaccgtaat ctccgctatc ccaccaatcg ccataaacat tttcctggaa
     4441 ccgtgcatct tccttgtaat aagtcaaagc ccgtccttta gacagtaatt gtatatgacc
     4501 atctgtgttt tcaggtagga cattcccaac tgaatctgta atccgagcgc ttgtcatgtc
     4561 ttccagtcca acgcccatat ctcttgcatc acttattttc aaactctcta gtgtatgagc
     4621 cttaagaatc attggtccgc attcactttg gccatatact tgaagaaata ccgcattctg
     4681 tgccatcgac gttcgcaaaa atgtcttcat tgtctggttg tttatagcat caaaagtgga
     4741 gtgatagtat cggatacccg aaaaagcttc tggatgctcc ttagcagtaa aacgccactg
     4801 aacaaagtta ttcggatggg tctctagtgc aattggccgg tgaacttcca acatatgaac
     4861 aacttttgaa ctttgtgcgt ttgctaaagg catcatagga aatcccatgg acatcaaaga
     4921 agagatacca atattgaatc tggaatgcac tggagagatg tgaaaagcga ttaatttctt
     4981 ttccgaaatt tttgtgaaaa ctgttttttg ccatttcgtc cgccatccca tagaatgagc
     5041 agaatgacaa ataagtttag ggataccagt tgtaccagaa gtatgggtca tataagaaat
     5101 ttcatttttg tctaaaaatg attgagaaac agacaaatca ggttgttcca ttaaataaag
     5161 aataggaatc tgttttgatt ttgttccatt tgaaatttct ctcactcgct tttttgtctc
     5221 atcatcaaat aaaatgaaag ggtcttctaa acgatcgata aatacttcca ttgttgttgt
     5281 tggtagatga taagaagtca tgacaggcac tgcaccaagg taagacacag aaacagctag
     5341 aaggtatgtg tcaaaagcgg aactcttata aataataact ttgtctccgg atttcactcc
     5401 aagatgagcc aattgacaag cccgtttcaa cacactttcg cagcattttt tgtaagttgt
     5461 ttcaagtcct aattctggga aagctgaaag tggctcatca aagataatcg gggtctcgga
     5521 aaagttagct gcagcttctt gaaaattcgt aaataagttc aaaggggaat acgttatggt
     5581 ctccatcatt cacactccat tatcttttgt taagtaatac acaaattcaa gataatcgta
     5641 acataatcct ttatatagta acatagttta ttgtgaaaaa aagatcatac atttgtttta
     5701 tatggaagaa acctgaacga atgccgctaa ttcgtgataa gaaaaatcaa ccatcttttc
     5761 ccagatatct tgaaaagaat gggatgagag gtatcgatta ccacataaat aatttaccgc
     5821 gtcagcatca tctaaatata gttctgcata ataacgatat gtttttctgc atagttctgg
     5881 cgatacgttt ttatatactg taattttgct caaaatttgg cgaatagtcc gaggagaaat
     5941 ttgctgatca tgtgaggaac gaaaaacaaa agaattttcg tccgtatttt gtgctgttaa
     6001 ctcaaaatag ttctccagaa acggcatcgc ttcttttggt attggtactt tattcagtct
     6061 gttttctttt ctattcagat taagaatctg cttggtcaaa gatacatctt tacgtttaag
     6121 ttctactact tcatggatct ttaatccgca atacagaaga atggcagtaa ttgcaaggtt
     6181 cctcggtcga ttcttttctt caaagctagc ttctctgctt gttcgaaagc cttcaatcgg
     6241 gataaacaaa tgctcaaaat ctttgaaaag ttctgcatcg atatatcgcg gagctgcttt
     6301 ggttgtttcc ggtaaacttg tacccaaaag agaaatctct tcttcaatca gtcctttttg
     6361 atacaaaaat cgtaaaaaat gatttaatga atattgcttc cttgaaatcc cgtaatagct
     6421 attttcaaac aattgctccg tttcttggtt cgctgtagtt tggaacgaac gagtatgtcg
     6481 atagatttta gctaagaaat tctgataatc ttgtttttct aaatggataa tcagctgatc
     6541 atccacttct tcagaaaata cttttctcaa ataatcagta aacagatgaa aatcttttgc
     6601 gtattcatag cgactgccta atgaaaggga agaacgattt tcttctaaaa actgttgaaa
     6661 gacggaaggg agcaagtgtg tacgttgctt taacttttct tgcagttgtt ttctagtttt
     6721 gctgtccatt acaaattccc tccaatagtg ttcattttct atattttacc gcatttcatc
     6781 aaatgaagat agacagtttg tcttgatctt aggaagcaaa tttttaactg tcttttaaaa
     6841 gtactagcta aaagatttga agtaagaaga tacattttta gttattttat tagtgcagtc
     6901 attcttttga tattttaatt gatttatcct ctttttagct atgtggttac ttcatgagca
     6961 atgaaatgga tttggttcgt tctaatggca ggaggctttt tcttttattt ccgaaaaatt
     7021 agaaaacatt ttttcagaat cgattggctc tccatttatt caataatagt ttgtctaacg
     7081 atattgttgg gataattctt aaataagtat tagaaatagt tggtacttca ttactttaca
     7141 tgaagattta ttctatggta ggagcaatta tactgagaat taatcctttt ttatataagt
     7201 tgtttgctta ttgatggatt ggataaatta aaccatcaca acgagtggaa attatatgaa
     7261 atttttcata aaaattcata tagaaacaaa tgacatttta ttagaatatg actttcatta
     7321 acataaatgt tttcaaattt caattagatt gttttaatta aattccaatc taaatatttg
     7381 tagaaaacaa tcgtctccta tagaataaat ttataagtaa caatatacca atcataaatc
     7441 tatttgctag gaggaattta catggaagag aaaaatttaa cggtcatcgg aagctatagt
     7501 acgagggaag aagcattatc agtaattgaa cggttgcgta acgaagggta tgaacgagat
     7561 gatatcgtta tttatactac tgatgaagca gcatctagac taggatttga cggattgtct
     7621 ggtatagatg ttgaaacaga tgaaaatcgg atggatggtg aagaagaccg ttctttatgg
     7681 gaaaaaataa aagatacttt ttcttttgat acctatgatt ctgagacggc tacaccagaa
     7741 aatgatactt tatatcaata tagaggagat atttctgatg gaaaatttgt catcacagta
     7801 aaaggctatc gtcagccaga aacaacagaa gatacaatgg atacacaaga tactgttagc
     7861 ccagcaagca caatggggac tggattagaa actggcactg gaacagatcc aatggacgta
     7921 ggtggcacga caggctttca aaatacaacg gatccattga atatgacacc aggaactgat
     7981 ccgatggacg tgcctggcga gactgataca atgggtacaa gcaagactga agacatcgat
     8041 cgcaaaccaa atctagatga tgatacaatt caactaaaag aagaaaaatt agatgtaaat
     8101 acacatgatg tgacaactgg agaagtcgac atccacaaac atgtggtcaa cgatactgaa
     8161 acggtagaag taccggtcaa acgtgaagaa atcgtcatcg aacgcaaacc agtaacagat
     8221 caatcttcac agggaacaga cgaaaacttg gaagatgaca cgattactat cccaatcaaa
     8281 gaagagcaag tagatgtaag caaacataca gttgttcgtg aagaagttgg gattcataaa
     8341 gaagaacatg aagatgttga aaaagtgaca gaagatgtta gccgagaaga attggatatt
     8401 gacacaagcg gtgatgtaca catggaagac cgcaataaaa aatcgtaagc gtaagctaaa
     8461 gaggttgtga cagaagtggt cagcttaaag aaatttcggc ttatttccga agaagctact
     8521 tctggaacac cgtttattcg caacaaaaaa gggctgtgac aagatttttt gtagtgaccc
     8581 ccaaaagtta gacttttttg gagtggagaa atccactcct tttttcatgc tctaaaatga
     8641 tatttttaca caaccgttgc ggttgtttta cgaaattgga cg
//
LOCUS       DAGCWU010000123         8674 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.123, whole genome shotgun sequence.
ACCESSION   DAGCWU010000123 DAGCWU010000000
VERSION     DAGCWU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.123"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(5..535)
                     /locus_tag="MBZ71_002146"
     CDS             complement(5..535)
                     /locus_tag="MBZ71_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBT4430068.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(634..984)
                     /locus_tag="MBZ71_002147"
     CDS             complement(634..984)
                     /locus_tag="MBZ71_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBT4430069.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1141..2670)
                     /gene="ahpF"
                     /locus_tag="MBZ71_002148"
     CDS             complement(1141..2670)
                     /gene="ahpF"
                     /locus_tag="MBZ71_002148"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBT4430070.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2680..3243)
                     /gene="ahpC"
                     /locus_tag="MBZ71_002149"
     CDS             complement(2680..3243)
                     /gene="ahpC"
                     /locus_tag="MBZ71_002149"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBT4430071.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3387..4049)
                     /locus_tag="MBZ71_002150"
     CDS             complement(3387..4049)
                     /locus_tag="MBZ71_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4430072.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4233..5879
                     /locus_tag="MBZ71_002151"
     CDS             4233..5879
                     /locus_tag="MBZ71_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBT4430073.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIVEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5870..6952
                     /gene="uxuA"
                     /locus_tag="MBZ71_002152"
     CDS             5870..6952
                     /gene="uxuA"
                     /locus_tag="MBZ71_002152"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBT4430074.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7060..8613)
                     /locus_tag="MBZ71_002153"
                     /pseudo
     CDS             complement(7060..8613)
                     /locus_tag="MBZ71_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2777 a         1581 c         1570 g         2746 t
ORIGIN      
        1 atgattaacg tatagtcatt tttcttattc tgatgaaatt gataatctcc tggatgccca
       61 tgaaaatcaa aataaagccg aatatttgga atagaagcat caaacttttg aatggattga
      121 acaataagac gaatccgcca atcaacaaca gaatactact ataaacactc caacttttag
      181 cttttgtatt catgctgaag aacaactgga acaagctgtt gatgataaca gctaatccaa
      241 gaagaatcgg caggatagaa acgattgcag gtgcaaaagc atagacgatc agagctaaga
      301 tcaggaaaaa aagaccactg aacagaccga tactccaaga acctgttttc cttttaaaac
      361 ggtaagcctc aaaaagattc aacaacccaa gcaaaacaag ataggcggtg atcagataac
      421 cgataaaatg aaaaacagca gaagggttta gcacaatcgc aatcccagaa ataatataaa
      481 cagcagctct taataaggcg tatttttgaa tattttgttt aatcgtctca aacatataag
      541 aaaacctcct ttgacttatt ttaacgtgaa ctagtcattt taccaaatta aatgttacaa
      601 aatgagcgaa ggagaagaat atctctggaa aaatcaactt ctgaggatct tttccatttt
      661 tttgcctttt gctagctcat cgatcatttt atctaaataa cgaatttctt tcatcaagtt
      721 atcctcgatg tcttctactc gtacaccaca aacaacacct gtaatcaagg aacgtgaagg
      781 attggggaca ggggcttccg caaaaaattg ttcgaaagtc acttgttttt gtagttgata
      841 ttctagttct gtttgggaat aacccgtcag ccaacaaatg atttcatcta cttcctcttt
      901 tatacgatct tttctctccg cttttgctac atacatagga taaacgcttg caaaacttgt
      961 cgtaaaaata cgattttgcg ccataatcag tcacctttct ttttttcaaa ttatttacta
     1021 cttatagtat ataagaaaaa gagaagaaaa agcgattatt caaaaaaaca aaaaggattg
     1081 tgacaaaatt cttgtcacca tccttttttc cgctctctga ctttttacaa tcttcatagc
     1141 tcaagtgcgc atcagataat cgaatgctcc taaagctgca gtagctcctg aacccattga
     1201 aacaatgatt tgtttgtatg gactatctgt acaatcacca gctgcgaaaa cgccggggat
     1261 attcgttgcg ccatttttct cggtcactat ttcatttctt ggattcattt cgatcgttct
     1321 atctagccaa tccgtattag ggagtaagcc gactagaatg aatactcctg aaacagctag
     1381 tgtatgcatc tccatcgtac ggcgatcgat ataggagaga gattctacct gattcgtacc
     1441 gctgatttct ttcgtcgctg catgtttgat aacagtgaca ttcgcaagag agactagttt
     1501 atcctgtaag atttgatctg cttttaattc tggtaagaat tctagaacat atacatgttt
     1561 tgctaaccca gccaaatcga ttgctgcttc aacaccagaa ttaccaccac cgataacagc
     1621 aatctctttt cctttaaata aagggccgtc acagtgtggg caataagcaa ttcctttatt
     1681 tttaaactct ttttcgcctg gtacattgat agaacgccag tgcgcacctg tcgcgatgat
     1741 ggcggttttt gctttaagtg atataccgtt agctaagcca atattaataa aatgttgctc
     1801 tttatttaag gaaacggcct gttgattcat cataatgtcg atagggtaac tacgcacatg
     1861 actttctact tgctgcatca attttggacc ttctgtgtaa gaggtaccaa tcatattttc
     1921 aatccctagt gtttcaacga cttgtccgcc gaaagaatca gcgacaagtc ctgtacggat
     1981 tcctttacgt gctgcataga tagctgcact actagctgct ggtccaccgc caatcactaa
     2041 tacatcataa ggttcttttt caaacaactc ttcttcaatc aaagggccag tgattttttc
     2101 aacaatttgt tcgatcgtca tgcgtccgct actaaattct tctccattta aaaacacagt
     2161 tggcactgcc atgatttttt tatcatcgat ttctttttgg aacatcccac cttcaatcat
     2221 cgtatgagta atatttggat ttaatacagc taatatattc aatgcctgta caacgtcggg
     2281 gcaattatga caagtcaagc taacataact gtcaaaatgc aatgtttgtg ttattttttt
     2341 gattttaaga ctcaaagatt cactgatttt cggtgcccgt ccactaattt gtaacatagc
     2401 cagtaaaaaa gaggaaaatt catgaccgag ggggatacca gcaaaaacga ttccactttc
     2461 tttgccaact ggattgatag aaaaactagg tgtgcgcatc gaatctcttt tttcccaaga
     2521 gattttagga gacatttctg ctatttcctt cgtaaattca gctagttttt gactattttc
     2581 actattatcc aaactaacag aaaaaacaat aggagattca atcagtgcta aatattgttc
     2641 gagattttgt ttagtttctt tgtctaacat atccaccacc taaatctttc caacaagatc
     2701 aaaacttggt ttcaatgttt cgccactttc tttccatttt gccggacaaa cttcacctgg
     2761 gtgggtacgt acatattgag cagcgcgaat cttatcaatc agtgcactag cgtcacgacc
     2821 gattccatct gcattgattt ccatagcttg aacgattccg tctggatcaa taataaatgt
     2881 tcctcgctga gctaatcctt gctcttcatc caaaacatca aaaatacgag aaatctgatg
     2941 agacggatca ccgatcatca cgtattcgat tttaccgata gcatctgatg tatcatgcca
     3001 tgctttgtga gtaaagtggg tatcggtaga gcaagaaaag acttcaacac ctaaagattt
     3061 caatgtactg tattgttctt gtaagtcttc taattcagta ggacaaacaa aagtaaaatc
     3121 agctggatag aagcaaagaa tactccagtt ccccataaga tctttttcac tgacttctaa
     3181 aaattctcct tgatgataag catgtgcatt aaattcttcg atttttttcc caatcaatga
     3241 catagtaaaa cctccaataa taattattta tttataatta ttgtaattta gttggagttg
     3301 caaagtcaat ggatattttg ttatttaata aagattactc aaaaacgaag gttaattata
     3361 aaaatagaag aggtcgcaag aaaatgctac tgctggaaat actcgggata acgttgagta
     3421 acagtttcct gttctttcag catcatgtgt aagtgcaatc ccgtttgaaa tcgagcttct
     3481 tctggttttt tatcaactaa agccttgagt aaatcataat gttgttccat gatcgtttcc
     3541 catggaagcc cctgaatggt cactcgtaac cagcggaaac gttctaaatg ggtattatgg
     3601 tcatctaacc agttccaaac ttcttttctt cctgcaattt cgaaacaagc ttcatgaaat
     3661 aaattatctg cacggaaaaa agcgcgttcg tcatttattt tgactgcttt ttcttgttct
     3721 tctaaaatcg tttctagcac tattcgtgtt tgtgtgtcca atttggaaca acattcaatc
     3781 atgatttgct cttccaactg ttctcggaca aagcgagcat tttctgcaga aacaagatta
     3841 attttggaaa catacgtacc actttgaggt atcgtataca cgagttcttg ctgacgtagt
     3901 tgaatcaatg cttcgcgaat gggagtccgg ccgatagcaa gatgttcctc cagtgccttt
     3961 tcagatattt tccttccagg ttctaagtca gcatagataa tttgtttacg aatcgcttga
     4021 taagcttgtg attgcaagct gcttttcatc tttattcctc caaaatcatt tctatgaacg
     4081 tagtataaca gaagaggtct gaaaaacaat ctggtatgtc acattaacta gtaaatttgt
     4141 ataaatagta aaattttggt tgacatagcg atttttattg cataaaattc acttgaatac
     4201 aaaaactaat atgttagttt aggaggggaa aaatgattga tttaaacaac tggaggaact
     4261 ttatccctga attatctcaa aaagaagtag aaatacctca ttttgaccgt gcatcattaa
     4321 gagaaaatgg acaaaaaaat cctgtctgga tccatttcgg tggcggaaat ctttatcgag
     4381 gatttcatgc agaaatcgct caacgtttag cggatctagg cgaattgtcc actggtgtca
     4441 ttgtttgtga aacattcgat gaagaaataa ttgaaagggc ttaccaagat tttgatgatt
     4501 tgattcttga agtggtcatg ccaagcagtg gagagttcgt caaacgtata ttagctgcga
     4561 cagctgacag tctgtattgt catccagatt gttctgaaca ctttgataag atgattcgac
     4621 actttgaaaa tcctagttta caattagtta cttttgctat tactgaaaaa ggttatcgat
     4681 tgaaagatac aaataataaa tatataggac cgattgcagt cgatataaaa aatggtccaa
     4741 agaagccatc acatacaatg agtattcttg ctgtgctact ttataaacgt tttttatctg
     4801 gagcttggcc gattgcactt gtctctacag ataatttttc acaaaacggt agtcaattac
     4861 gtacttcttt gctaacgatc gcaaaagaat ggcaaaagga aggttatgta caaaatacat
     4921 ttatccaata cttatcgaat gaagaaattg ttgcgtttcc atggacgatg attgatcgta
     4981 tcaccccgaa tccttccttg tcagttagca aaatgttggc aaaagagggc ttttcaaata
     5041 tgtcgatttt ccaaaccgca aaaggaaata atattgctcc atttgtcaat acagaggaca
     5101 tccattattt aatcatggaa gatcattttc ccaatggacg tccgaaattc gaaaaagctg
     5161 gggtattctt gactgatcgt gcaacggtag ataaagcaga tacgatgaag gtgacgactt
     5221 gccttaatcc tttgcatacg tctttagcta ttatgggttg tttactgggg tataggagca
     5281 tagttgagga gatgaaagat ccagatttat cgctgctagt ttctgaaatc gggtacaatg
     5341 aaggcctccc agttgtggag tctccaggta tcattgatcc aaagatcttt ctggaagaag
     5401 taattactgt gcgcttttct aatccaaata ttcctaatat gcctcagcga atcgccacgg
     5461 atacctctca aaaggtggcg attcgttatg gtgagacgat taaaaaatat atggaaatag
     5521 aagttaaaga cccgcagaca cttgtattca taccattgac tctcgctgct tggcttcgat
     5581 accttattgg cattgatgat caaggaaaac cattcatccc gagtccagac ccactattga
     5641 cagaccttca gcataagctt tccgatattc aactgggtac gcaagataca tcacatattc
     5701 atgagaaaat ggctcctatt ttgtcaaata aagaaatttt tggtgtagat ctttaccaag
     5761 cagagcttgg cagaaaaata gaagaaatgt tcatcaagat ggtagcagga ccaaatgccg
     5821 tacgaaaaac attacaggat tacttacaag aacatggagg aaatttatca tggaaatgac
     5881 ttttagatgg tacggatcac aggaagaaaa gattacctta gatgaaatca gacagattcc
     5941 tgcggtcaaa gggattgtag gcacattgat ggatatacca gtaggagagg tatggcctag
     6001 ggagaggatc aaagaactaa aagaagaaat tgagtctcgt gggttaacac taaaagtaat
     6061 tgaaagcgtc aatgtgcatg atgccattaa aattggatta ccttcccgtg agcagtatat
     6121 cgaaaactat aagcagacaa tccgaaactt ggcagaatat ggaatagaag tcatctgcta
     6181 taattttatg cctgtatttg actgggtgaa atctgacctt gactaccgct tagaagacgg
     6241 ctcatccacg ttagctttta tatctgatga tattccagat gatccaagag aaatcgtcgc
     6301 gcgcatcgaa cattccagca atgaatttgg attgccggga tgggaacctg agcgcttagc
     6361 tcctatcaaa tccttgcttg aagcctatgc atcagtagac gaagaaaaac ttcgagaaaa
     6421 ttttgcctat tttctccaat caatcatccc aacatgtgaa gaggtagggg tcaaaatggc
     6481 tgtacatcca gatgatccgc catactcgat ttttggatta gctcgaatcg taaagaatcg
     6541 agaagattta gattggttgt gtcgcatagt agacagccct tataacggaa tcactctttg
     6601 tacagggagt attgcagaag atccagaaaa tgatgtatat gggattttag cagaattttg
     6661 ccaaagagat cgaatcccat ttgcacatgt tcgaaatatt aaatttttag ctaatcgaga
     6721 tttctatgag gcaccacata aatccgaata cggttcatta gacatgtacc gcatactaaa
     6781 aacaatgtat gacaacggat ttaacggata catccgtcct gatcatggaa gaatgatctg
     6841 gaatgagaca ggaagaccag gatacggctt atatgatcga gcattaggtg ctgtttatct
     6901 aaatggatta tgggaagcaa tcgaaaaagg aaacaaaagt aaagataatt agtgaaaaaa
     6961 actgtaattt tattatgtaa ataagaagga tgttcaaatt cttgatacat aaatccggag
     7021 gttgttaata agcattcgga tttatgtatc ttatttggtc taggaagtaa cttgattttc
     7081 ttttttcgat tgatccatga gtgcttcttc ttttaattca aagatatatt ctgagatcct
     7141 cataatcact tttttccagt cttctggtgt gatgattcct tttaaaatat agattttttt
     7201 gatatgagaa ggcaattgtt tcaaagggca gtcgctgata acgatgttgc agttctccat
     7261 aaaatcattt tgtattttga tcgattcatt tgaaaaaatt tgttctatct taacgatcag
     7321 atctttactg atataaaaat cacgcgtata gttcacacca atcacaattc gcggctgttt
     7381 tttgtagcga tcatacaaat gtactaggtc ttcaaccatc cattgcacat tttccttttc
     7441 taaaataact ggtaacaagt gttgattttg ttctctgaaa tccatataaa attgttcaat
     7501 ggcggtttgg acttctttca atacgctatg ttcttgataa agcatgaaag taggaagggg
     7561 cgtatttggt tgattgaagt taagaaatcc attataaagt ttatttcgtg cgaatgccaa
     7621 tgagtaaaga atcttttctt tttcaggtat aaacaaatca aatacagtac aaagggactc
     7681 caccatttct cttgaataaa gaacatgagg aacgtttgct ttttttaaaa gtttgtattg
     7741 ctccaagctt gtttcttccg attcgatttt cgtgatagaa agtcgagcta aaatattcac
     7801 taagatgcgt tgttcgcgac aaattggcag attaggtcgc aaaatatcta cagagagatc
     7861 aataaaaaag tcaatatatt catactctgt gaattctttt tgtacgtctt tcacagaagt
     7921 tgcaggaaaa ttctctttac atatttttaa tagaaaatat aatttatcta tcataccgct
     7981 atttagtcgg tcaagtactt cttttttaaa aaaagtattc atcataaaag ccactacttg
     8041 cggatcttca tcataaaatt ctgtagcagt acttgaaaag atgttccaat aaatatctaa
     8101 tgctgcatat tgaatttgta cctcttttcc agaaaaagac tttctgccat tattagaaaa
     8161 ggagatagac aaaccgtact atgctaaaaa tgcattaatc tttttcattt tcgtatacaa
     8221 ataagaggca ctatagtttg tttgctgact gaaactgata atcgaatttg tctgacagct
     8281 gaacaaagaa gtcaagatct gataactgac tgattcgttg cagtatcttc caaatagacg
     8341 ataatagcag taagccggat caattccttc aaaattcaac tttaaaaaag gtttttgctt
     8401 atcaatatgc agttctgtta gattgagacg ctcaaagtct tgttctaatt ctttgatgta
     8461 tagtaaaaaa tttgattcag acatatggaa atgatctgct aaatattgtg tagtaataaa
     8521 gaaacgatgt tcagatagga aaagaaaaat atctaatttt gtattgcttt ttttcttcaa
     8581 gataaacgtt ttaatcgctt cttgagtttg catgaaagag gctccttttt acttaaattg
     8641 ttaaaacttg aaaatattac tgtttttttc ataa
//
LOCUS       DAGCWU010000124         8536 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.124, whole genome shotgun sequence.
ACCESSION   DAGCWU010000124 DAGCWU010000000
VERSION     DAGCWU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.124"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            69..356
                     /locus_tag="MBZ71_002154"
     CDS             69..356
                     /locus_tag="MBZ71_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430075.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            772..2310
                     /locus_tag="MBZ71_002155"
     CDS             772..2310
                     /locus_tag="MBZ71_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBT4430076.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2307..3272
                     /locus_tag="MBZ71_002156"
     CDS             2307..3272
                     /locus_tag="MBZ71_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4430077.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3259..4581
                     /locus_tag="MBZ71_002157"
     CDS             3259..4581
                     /locus_tag="MBZ71_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4430078.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4644..4748
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4822..5565
                     /locus_tag="MBZ71_002158"
     CDS             4822..5565
                     /locus_tag="MBZ71_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4430079.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5580..6572
                     /locus_tag="MBZ71_002159"
     CDS             5580..6572
                     /locus_tag="MBZ71_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4430080.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6584..7321
                     /locus_tag="MBZ71_002160"
     CDS             6584..7321
                     /locus_tag="MBZ71_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4430081.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7311..8033
                     /locus_tag="MBZ71_002161"
     CDS             7311..8033
                     /locus_tag="MBZ71_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBT4430082.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8117..>8536)
                     /locus_tag="MBZ71_002162"
     CDS             complement(8117..>8536)
                     /locus_tag="MBZ71_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBT4430083.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2760 a         1464 c         1839 g         2473 t
ORIGIN      
        1 tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga
       61 gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag
      121 agtatcaagg agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga
      181 taaataaagc aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga
      241 aagacatctt tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag
      301 aagaactcat tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa
      361 aaataagaaa acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa
      421 aagtgtcatg atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg
      481 agatttctac tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag
      541 atatagctga ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa
      601 cgagaaccaa aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta
      661 gaggtatttt tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg
      721 gaaaaaggaa agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat
      781 tttatacaaa cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg
      841 tccatttctc tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg
      901 gcagccaatc ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg
      961 attccttcac ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca
     1021 gcattgattg cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt
     1081 ctagctgaaa ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg
     1141 agacgcttgc tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga
     1201 actgccttag ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga
     1261 ttagggacat ttattttgtt aggtattgac cgaaatacac ctgttttgac attgattggt
     1321 gcgatcagtt ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat
     1381 aagaaacctc gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt
     1441 tctttatatc agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga
     1501 gcggaacctg aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca
     1561 gaagttgaat taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa
     1621 ggtcaaatcg atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta
     1681 cctgaagcaa tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa
     1741 ttattgaata agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat
     1801 gccttggcag tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg
     1861 gcagctatct ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat
     1921 ggatataaag gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag
     1981 ccaagtttgc gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc
     2041 acagatagcc agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc
     2101 ccagcttatc aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc
     2161 gttaaagcac tgaataaatt aagcggaaaa ataacagagg atcaaatgat tgatatgaat
     2221 taccaagtca atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat
     2281 gaaggattag taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa
     2341 aaaagtatga tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa
     2401 tctttgtttt ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg
     2461 gattatctaa accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata
     2521 atctgcagaa aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga
     2581 ccatgacagt caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga
     2641 aaagagcaga ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc
     2701 gagatcgtat gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag
     2761 ctattgctgc aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga
     2821 tttcaagaaa ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca
     2881 tcgttttcgt tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta
     2941 tgaaggatgg agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa
     3001 acgactacgt tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag
     3061 atttagtcgt caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt
     3121 gctttgatac gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga
     3181 tagtagaaaa gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa
     3241 gtcgagagga ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc
     3301 ggcttaagtg ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa
     3361 gatctatggg gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt
     3421 gcagtagagg cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca
     3481 actgccaact gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa
     3541 agccgaaaaa aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt
     3601 ttggatgatt catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg
     3661 gcaaccgggc aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt
     3721 gcagactttt tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat
     3781 attgcttttg agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat
     3841 cataatcaac ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa
     3901 atggaagcat ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa
     3961 ggtactcgct atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt
     4021 gcgactggga gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt
     4081 gataaacacg gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg
     4141 tgtggagata ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga
     4201 aattatgtag ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata
     4261 cctacgatcg tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc
     4321 tcttcgtcag atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc
     4381 aatgaaccag ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct
     4441 gcagtgatta gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa
     4501 aaattgacta aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat
     4561 ctctcctatt tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct
     4621 tgtgctacac tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt
     4681 aagttgtgaa cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag
     4741 cagacgcttg aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat
     4801 tagaagaaag gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc
     4861 attttaatta tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc
     4921 ggacctgctg ctgtattttc tgcattagcg gcagatgcac aagtattact ttcccattct
     4981 gtcacgacat taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt
     5041 actgccttgg caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg
     5101 atttcacaaa tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc
     5161 ggtatgcttc caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct
     5221 ttttcggata cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg
     5281 gcagatacag ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt
     5341 tccggtttaa aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta
     5401 agcgctcaag caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat
     5461 aaagtttttg ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt
     5521 tcattattga aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa
     5581 tgaaaaaatt atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa
     5641 aagaaacaag tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt
     5701 atgtaccgaa tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag
     5761 aggctggttt aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag
     5821 taggtgcgga caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg
     5881 cagatggaca gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg
     5941 gattcgccac cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat
     6001 atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg
     6061 gcggggactt ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag
     6121 gaaaatcctc agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag
     6181 cgggtattga actaaactat atcccattga aagaattaga tgaacggtta gattactata
     6241 ctccggtaat tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt
     6301 ttatggatgc cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta
     6361 aactgttgca aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat
     6421 tcttgtcaaa aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt
     6481 ggcgcaatta tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc
     6541 aagaactatt cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag
     6601 ccacttgaat gtagtcattg aaaacctgaa aatattagaa gacatccatt tcactatccc
     6661 taaagatacc tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa
     6721 aactttaaca ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt
     6781 aactggtctc catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct
     6841 gagcgtctat caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga
     6901 acaagtaagg gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga
     6961 acaattatct ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc
     7021 ttcttacttg cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat
     7081 gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca
     7141 tgatatcgaa gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc
     7201 acatgtgatt gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca
     7261 gcaatcagag ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg
     7321 atgctcattg tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga
     7381 ttttgacgat catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag
     7441 caagtaagac tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt
     7501 ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata
     7561 atatttggac gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt
     7621 ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct
     7681 taaattatat ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact
     7741 atcaacaaga ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga
     7801 atccagcagt ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg
     7861 atggactaca tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct
     7921 cactgcttga agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag
     7981 ttaaaaaaaa tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag
     8041 atgtgacaca actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt
     8101 gattaataaa tgaatcttag taccaaccgt ttgcttccca gaatgctttc gccgcttccc
     8161 aagaaccata acgtgaagta acatattgtt ctgctactct ttcttggttt gcagcagagt
     8221 agtcaccatt taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt ccgttcgttg
     8281 ctgtgtaaga accgcttgat tctttttgtg cgatccattc ttttgcagag cttgtgcttg
     8341 ctggagtagc agcagtttct gttacagcag gagcaggttg tgtttcagca acaggcgctg
     8401 cttgtttttc cgtttgaacc gtttcttgta caacaggttg ttcagcttgg actggtgctt
     8461 cttgaacggc tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt gggattgtta
     8521 attgttgtcc tgagta
//
LOCUS       DAGCWU010000125         8489 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.125, whole genome shotgun sequence.
ACCESSION   DAGCWU010000125 DAGCWU010000000
VERSION     DAGCWU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.125"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            1..2373
                     /locus_tag="MBZ71_002163"
     CDS             1..2373
                     /locus_tag="MBZ71_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430084.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2434..3894
                     /locus_tag="MBZ71_002164"
     CDS             2434..3894
                     /locus_tag="MBZ71_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBT4430085.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERSNGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3905..6109
                     /gene="topB"
                     /locus_tag="MBZ71_002165"
     CDS             3905..6109
                     /gene="topB"
                     /locus_tag="MBZ71_002165"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBT4430086.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            6125..6274
                     /locus_tag="MBZ71_002166"
     CDS             6125..6274
                     /locus_tag="MBZ71_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430087.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6355..6951
                     /locus_tag="MBZ71_002167"
     CDS             6355..6951
                     /locus_tag="MBZ71_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBT4430088.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            6964..7863
                     /locus_tag="MBZ71_002168"
     CDS             6964..7863
                     /locus_tag="MBZ71_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBT4430089.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            7866..8351
                     /gene="ssb"
                     /locus_tag="MBZ71_002169"
     CDS             7866..8351
                     /gene="ssb"
                     /locus_tag="MBZ71_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4430090.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT         3200 a         1444 c         1814 g         2031 t
ORIGIN      
        1 atggcagaag aaaaagaacc actagacaat agtcgtttgg gaaaatccaa aagaaaacta
       61 gtacgattgc aaaatgaatt aaacgaacaa attgaaaaga tgtttgaaca ccaaagaaag
      121 accaacggtc aaccaatgaa cgataagaga aatgggcatt cttggtttag gcaacaagag
      181 cgaatcgaaa ataaagtgca tagtctgaga gaagagatca agcaacaaga aaaacaggta
      241 gaaaaacttg aaagacaaga agagctaaag gaaatgggct ataacaagta cggcggactg
      301 gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
      361 gaatcgactt tttcagccgc cacgatccga aaataccaac ggaagttgga aacattagaa
      421 cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt acaagcgatc
      481 attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt tttgaaaggc
      541 tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc aaaaaaatac
      601 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgcacca
      661 actgattctc tgaaggagaa agaaaaagat catttctatc gagtagaatt caacgaaaat
      721 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattaga gttgatcgat
      781 caacttaaaa cgttggataa ccaatacgct gcagaatcag gttattataa attctatttt
      841 gaagaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
      901 aacagtaatc aacccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
      961 gaacaaaaac aatcggtcaa aaatgaacct gttacgaaag aacaaatgca gtcgaaagaa
     1021 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1081 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc tcctgaggac
     1141 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1201 ttgattgagg aacaatttcc aggtgcaacg gcatgtttag gtcgtgccgc gttgaaaaaa
     1261 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1321 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac accagaagat
     1381 aaaaaacgaa tcgaaagtgg tcaaattgaa gtgatcaaaa aaccttacta cacgattgaa
     1441 aaagtgtttg atatcacaca aacacaattg aagcctgaag actatccgaa aatcttcccg
     1501 aaccgcgtct ttgattttca attggataaa acagggcaga cagaacttca agttgggatt
     1561 caagcggtag ccaaaaatat tggaattcaa attcgagata tgacagaaag tgaagtctat
     1621 caaagagaat taggacaagc acgtggggcg tacgttcgta acgaatttac tggcgaagaa
     1681 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     1741 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     1801 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtc ggagaaagct
     1861 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     1921 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     1981 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     2041 caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc tgttggttca
     2101 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     2161 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt aaaaggaaaa
     2221 agttatggaa aagagattca aacggcgtta ccttcagaag aattttcgct aggaaatcca
     2281 ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga aatctattat
     2341 gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact atctgaaaaa
     2401 cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat gagtcttatt
     2461 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
     2521 aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     2581 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     2641 tttttcatag acaaagagat ccaagactct aagcaacaat ttaaagcagc cacagatttt
     2701 attctaaaga atgaagacaa aacagaacga gtagaaaatc ttcattttga gacagaaaag
     2761 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     2821 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgcc
     2881 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
     2941 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
     3001 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
     3061 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
     3121 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
     3181 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
     3241 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
     3301 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
     3361 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
     3421 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
     3481 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
     3541 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
     3601 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg ctcgaatggt
     3661 acgtacgatt ttgatcgttt ttatcaagga aaaggggata ttaatgctca gattcttttc
     3721 tcttataaag cagaacaata ctacaaaggc tataaaaagc atgaacatga atttgtacct
     3781 gaagtgaaaa aggattggaa cgatcaattg aagcatgaga tccagcaaca agaaattaga
     3841 aagcaaaaac gtgcgatgtt gtttcaacaa ggcagacaac aagaaaggga gtgagatcgt
     3901 cacgatgaaa actgtcattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     3961 cttttctaaa gccacccgaa aagacgggta ttttgagatc caagatcgat tgtttcctgg
     4021 tgaaactgtg attacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     4081 cgatgaaaat tggaaacaat ggtcattaga acatttacct attttcccta cacattatca
     4141 ctatcatgtt cctaaagata agaaaaagca attcaacgtg gtgaaacagc aacttcaatc
     4201 ggccgacacc attattattg cgacagatag cgatcgcgaa ggggaactga tcgcttggac
     4261 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     4321 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     4381 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     4441 tagcctactg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     4501 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     4561 ccaaaaagaa ccatattttg aaggaaaatc ccaagtaatc gctcaaaatg gcgcatttga
     4621 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     4681 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     4741 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     4801 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaaggaaa
     4861 atatctaagc tatccgcgaa cggatacccc ttatattacg gaaggggaat atgcgtatct
     4921 gttggatcac ttagacgaat acaagcattt tttaaaagcc gaagcgattc cgactcctat
     4981 tcatacaccg aatagtcggt atgtgaataa taagaaagtc caggaacatt acgcgatcat
     5041 tcctacaaaa acggttatga cagcagcggc ttttgaacaa ctaagtccac tgcagcaggc
     5101 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     5161 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     5221 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     5281 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     5341 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     5401 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     5461 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     5521 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     5581 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     5641 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     5701 ctctcacttg ttggaagtcg taccaggaca aatccaatcc actgattttg gatcgacgtt
     5761 acaagaggtg aaagcagcca gtgaaaagca agaagccgcg cgtcatttag ggatttgtcc
     5821 aaaatgccag gaacaagaag ttttactcta tcataaggcg gccgcatgta cgtctgaagc
     5881 ttgtgatttt agattatgga caacgattgc caaaaagaaa ctcacagcga cacagctgaa
     5941 ggaaatcatt caaaatggcc gaacaagcca gccagtgaaa ggattaaaag gtcaaaaggg
     6001 ttcgtttgaa gcaacgattg ttctaaaaga agacttcaca acgagctttg aattttctga
     6061 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat ggaggggtta
     6121 tcttatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     6181 ttcagaacaa gagaaattac gtgccagtaa aagaagactt cagcaattaa agaaccagca
     6241 acggattcaa aaagaaattg aattagatcg ataaaatctt cctggctatt gtatacgcat
     6301 aaacttatgt tatactatat ttataagttt atacgtatac aaaggaggga gaaaatgaca
     6361 aaagcaatgg tttacaagct gctagaagaa tttaacggaa ctttggcttt gaaggatgcc
     6421 aaaaaagcgg gcatttctcc tgtaacgatc aaacgtatgg tagatcgtgg agagctggat
     6481 cgtgagtatc caggtttttt caccttacct ggtcaatttc ctgatgaatt gtttatggca
     6541 caaaagaaat atgagcgcgg cattatttcg catatcaccg cgttggattt atatgattta
     6601 acggatatga tccctagaca gatcgatcta accgtacctt acggttatca cgtttctgaa
     6661 aaggggttga aagaattcgc agttgagtta cactattcaa aagcggaatg gtatgaatta
     6721 ggtaagatcg aaataaagag tcgttatggg aatcctgtta tagcttatga tccagaaaga
     6781 accttgtgtg acatttggaa tccctggtac aacgttgagg atgaaatcaa agtaaaagca
     6841 atcaaaaact acatggagtc ggacagaaaa aatcttagaa aattgaatga gtatcgacga
     6901 attttgccta cagacaaaac gatacgctct tatattatgg ctttaaatta aaggagaaga
     6961 cacatgacac ctacgcaatt aaaagcaaaa gtgaagaata tttccagaga aaaagaagtt
     7021 gatccgcagc ttgttttaag acattttatg atggaaaaat tcttggaaaa aatctcagag
     7081 tctccttatc gtgaaaattt tgtgttaaaa ggcggcttct taattggctc aaagtatggc
     7141 attgaaaatc gaacaacaaa agacattgat acgactttac gggaaatgaa agtaaccaaa
     7201 gaaacattga caacggttct aaacgatatt ttttcaacgc cgacgaaaga aggcattcaa
     7261 tttgagattc aaggaatgaa agaaacaagg gaagctgatt attatccagg gtttagctta
     7321 cgtgtactgg cacatttgga aaacatgcgg ccagatttta aggtagacgt gacgacaggt
     7381 gactcgatct atcctgcaac gatcactcac agtcataaat tgatgtttga aaatcgaacg
     7441 attcctttgg aatcttatcc tacagaacaa attattgccg aaaaattaag tgcaaccttt
     7501 gattttttaa cagataattc gagaggaaaa gatttttatg atctctatac tattcctaaa
     7561 atggaaaaga tcgatgagaa gaccttgaaa gatagtgtga gaaatacatt tacacgtcga
     7621 ggaaaaactg atccgttaaa gatttattac gaaaaggata tggatgtact gaaaaatagt
     7681 catgagctaa aagatacctg gaagaaatat caaaaaagca atccttatgc tgcgtcgatc
     7741 acgtacgaag agacaatgga ttcgattagt aatttgatgg aaaaagtgat tcacgtcgaa
     7801 gaaaaagagc gaaaaatagc ttatcaaaga caccaacaga tgaacagaga aatggagaga
     7861 taaaaatgat caataatgta actttagtag gacggttaac caaagatcca gatttacgtt
     7921 ataccgcaag tggcacagca gtggctactt ttaccctagc cgttaatcgt aatttcacga
     7981 atcaaaacgg caatcgagaa gctgatttta tcaattgcgt gatttggcgc aaaccagcag
     8041 aaacgatggc gacattagct aaaaaaggca tcttgatcgg cgttgtcgga cggattcaaa
     8101 cacggactta tgacaaccag caaggacaac gtgtgtatgt gacagaagtt gtagctgata
     8161 acttccaact attagaaagc aaagcagcga cagagagccg tgcgcacgct gatcaatcgt
     8221 ccacgagtcc aagcaccacc acttttgaac aacgggatac agcgacgcct aacaacaatg
     8281 gattgaacgc ttcacagaat ccatttggtg gacaatcaat cgatattagc gatgatgatc
     8341 taccgtttta agtagcattt tctattggtt gcatttgcta cggtttatgt tataatttag
     8401 tcaacaaaaa gaattttcaa tctataacca aaaaccacta gctggcgggc tagtggtttt
     8461 cttttacgtt tctattataa gaattaacg
//
LOCUS       DAGCWU010000126         8212 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.126, whole genome shotgun sequence.
ACCESSION   DAGCWU010000126 DAGCWU010000000
VERSION     DAGCWU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.126"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(216..1844)
                     /locus_tag="MBZ71_002170"
     CDS             complement(216..1844)
                     /locus_tag="MBZ71_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430091.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            complement(1864..2067)
                     /locus_tag="MBZ71_002171"
     CDS             complement(1864..2067)
                     /locus_tag="MBZ71_002171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430092.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            complement(2068..2757)
                     /locus_tag="MBZ71_002172"
     CDS             complement(2068..2757)
                     /locus_tag="MBZ71_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HBT4430093.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(3012..3332)
                     /locus_tag="MBZ71_002173"
     CDS             complement(3012..3332)
                     /locus_tag="MBZ71_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430094.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(3365..4483)
                     /locus_tag="MBZ71_002174"
     CDS             complement(3365..4483)
                     /locus_tag="MBZ71_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430095.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(4512..5306)
                     /locus_tag="MBZ71_002175"
     CDS             complement(4512..5306)
                     /locus_tag="MBZ71_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430096.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(5316..6191)
                     /locus_tag="MBZ71_002176"
     CDS             complement(5316..6191)
                     /locus_tag="MBZ71_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430097.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(6408..6665)
                     /locus_tag="MBZ71_002177"
     CDS             complement(6408..6665)
                     /locus_tag="MBZ71_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430098.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(6668..6892)
                     /locus_tag="MBZ71_002178"
     CDS             complement(6668..6892)
                     /locus_tag="MBZ71_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430099.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(7065..8108)
                     /locus_tag="MBZ71_002179"
     CDS             complement(7065..8108)
                     /locus_tag="MBZ71_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBT4430100.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         2314 a         1680 c         1026 g         3192 t
ORIGIN      
        1 tcctagatta tctaaactta gcatataaga aattttttcc tgatctgtta gttttgaaaa
       61 tgaactttct tttaaacttt ggattataca atcagcacta aatatttcgt ttaataaagc
      121 agttttcttt tctaattcct tagaagataa agttagttct ctcatagtga cttacctact
      181 ttccaaattt tgtgtcctat ttatcacttc tctctttata taagtgataa aagatcgtaa
      241 atacgtttaa ttgttaagtt catattcaca tgattatcca aataaaatat cttatcttta
      301 ttagaaattt cactcataac acgataatat agaccatgat tcaagtaatc tgtatataga
      361 aaaatcgcat cacacgtatt caaattttta agtatctttc cattattttc gcttacatca
      421 aacattttaa tatttggtaa tagttcagtt aatttatttt gaaaatttac atgtcctccc
      481 actaaaagaa tgtttttatg ttttagataa gctatcattt cagacagtga aggctcttca
      541 gttttaccat ttaatgattg tctatgttct tgctcctgca attgctttaa tactttattt
      601 tcaaacctca gctgttcaag ttcttttctg attcctttta attctttaat ctcttgcttc
      661 aaaagttcct cttctttttt tgccgatttg actaaagatt ctaaccgacc gttttcttta
      721 ctcgcaatac ttaattcttt tcttaatttg attacttcag atttgttttc ttttctaata
      781 cttgcaattt gttttgctat ttcataatca gaattcagga tcaattcttc cttagcattt
      841 ttaaacaacc gagttaaaac gaactgatat aacaaactat aaaaaactag caaatagtca
      901 atttgtcggt actcggtatt ctgatcaatt gcatatagag agattaaaac gaaatacaca
      961 atatcattca gctcgctacc cttgatctca aaattattga ctaagtaact atcaataccc
     1021 atcaaatctg caaaatctcc atacaaagaa aaatatgatt ttgacaatat atctatatct
     1081 gaatcgtcaa acccatttac tattttccca tcattgttag tagttgctaa cgaagcaata
     1141 gtgacttttg aattgggttt tacaaatcct atacctaata gttctttcat ctttttaatt
     1201 tcaagtttgt tatccttttg ttcggcttta tgctgtttca tattgtaaag cggataaaat
     1261 gctgttccgt aagcaccttt tagtacgtta tctataaaat aatcttcaga acctgttttg
     1321 aatagaaagt taatcgcagc caaattagat aacagctctt cagtgttgtg catcaatttt
     1381 ttatcattta ataaattatc cccaagatat ttcattgcat aataagggtt tgtttcgcta
     1441 cttattttta atattttttg atagctattc ctcacgatat taaccaaaat atctgtctga
     1501 ttaattgaat tacagtacaa agccactcct aatgccatat aaacattcaa atcatgtttt
     1561 gccgtaccgt tgaataaaac gtcttcaact tttgaattat ctcgtctata ctttttgatc
     1621 atatttttga tattttgatc attctcttgg tatatttttt tcactcgttc caatgccttg
     1681 ggtttgttgg aaacagcaac agcgaagtct agttctttga aatcaatatt tagtatctgt
     1741 acctcatcac ctagattata atctctattt acgctcggaa aactgctgaa atctaactgt
     1801 tttttaccta tttttggcga aaatattcct tcgatttctg gcaaatgtaa tcacactctc
     1861 tccttattta tctttagaaa atttgtcact attctcctta tatttatcaa taaacctttt
     1921 taatcgagca atataagcgt taaccctttt aggttcttct tttcgccatt catcgtttac
     1981 gtgaccattt tcaaagaaag aagctaaaag agtttccact tcattaacca tatctgcgat
     2041 cgtatatttt tctgtaccct cactcaatta ttcatgaact gttaagtgtt catagctaag
     2101 ttcattcata attttgttat agcgttttct tgctttttca aattctttct gttcgtcaaa
     2161 ttccacttga ttcttcatgt tgttcttcct ctctgtttga tattttttca cttcttcaag
     2221 tttagcatct agttccgctt taacttctgt cacttcttta agcgcttggg ataacttatg
     2281 agacaatttc gcataagttt ctctcttgta ttcatttcct actcccaact cttcggagta
     2341 gaactcttct agtagtcttg caaaattaac atttctcctg ccagcatagt tttttagctt
     2401 ctcaaaatca gattgaaaga cagcaacact tttgtaatcc ttttcaccct caaaatcaaa
     2461 agcatagtca tactttcctg aaatcaattc atcaataact gtagcaatat attgagagat
     2521 agaaatatta tcttcacttc tatgtcgctc tataagatta tatatatcag ggctgacatg
     2581 aaacgtactt ctcttttttg tttgcctttt tctaactgtt acgaccactt ttggaacttc
     2641 actatctcca ttgatctctt tgtatttttc aataaataat tttctagctt gttctcgact
     2701 ttttgccgga atttcatcta caattttatc ttctaaataa atatagtgtg ttctcataca
     2761 ttcgtcctct atcttcttta ctctcaatct tcatgatctt agttcttacc ttgtttactc
     2821 ttaaacattg caaaaaaatc tagtcaaaat caaacttgaa atcatcgtct atcacaacac
     2881 ccttaacttc atctttagat ttcccaaagg aacccttatt ttcaaatcct ttatttttga
     2941 atgatgatgg ttatggaaaa cttcctttga aattctgaca agcataacta gtcagaactg
     3001 ttttacttag attaagcttt caaaagatac tgatgaagaa ctaagttctc agtaccttcc
     3061 cattcttcaa taaaaaatat ttctccttct tttacccact ctatagctaa tttcttataa
     3121 tcaaataaac catatgtgtt taagcctttg tctttcatta aactcaaaaa ctgatcccct
     3181 tttaatcttt ctgtatcatc catcagaaat tcaactaaat ctttatccat gcgtgcaact
     3241 ttttcgtctt ttagtgccat tgatttagca tagtcagttg aatcttttag aataataaca
     3301 cctacatttc cttccacaat tttcttcttc atatttacct cctaccatct tttccaaaga
     3361 gtatttagtc aaaatcaaac tcgaaatcat cgtctatcac aacaccctta acttcatctt
     3421 tagatttccc aaaggagccc ttattttcaa atcctttgtt tttaaattct aaagcttttt
     3481 cgacttcttc tttggatatt gtatgaactt ttttcttaac tactccaagt ttatacagct
     3541 cttcaaactc tggtacttcg gatctgaaat acaatccatc agtacgaatt tttaatagcc
     3601 catatgcgct atgaacgtat ttctgttcat atctaaccat acgatctcta aatacgcctg
     3661 ctaccatgga ggaatacgct cgattataca tactgtattt atcgaaggtg cttgtttctc
     3721 cttctcttaa tgatttttgt ccgatagcac caaatacttt cgttctaaga tttttataaa
     3781 gcttcaacgt atcaggatca gtttttaatg gctcgattct ttcaaaatat tctttgagta
     3841 atcctttttt cgtcaaaaat gtgtggctat ctaggacttc catctcttta atatcataat
     3901 aatcaaggat aagtgttagc tcaatctccg ataaccaaat aggctcatta gctgttttgg
     3961 gattttcgcc ataatccgca aacatcgact gtgattttgg attagcatat gttccttctt
     4021 tggctgttcc ttcaaaatta accaaaaaga acatatacgt ttttaaattt tcatacggtc
     4081 ttacttttgg tctatttatc gctctcaaaa tagcttgatc tgtattatat tttgctttca
     4141 aataagtgtt tgacggcaaa ggataatttc tcatgatcct cgaataggct tcgttgaaat
     4201 catattcgat catctttgct ccttcctcaa acaaatccaa gttatacatg ctaccggagg
     4261 tactggctct ataggctgaa cttggaacaa agaaaggtgc atcttccggc ttatcatagc
     4321 ccaaaaaatg tattcctgtt tgcttgttct gacggtaaat gtccatcaaa agatatttga
     4381 tcgtactaaa agaatctaat tttcctcttg tcatccaccc taatccgaca agtcttttaa
     4441 ttttattctg catttcaggt gtgtatatgc tattgtttcc catttatctg ctcatctccg
     4501 gcttttttat tctagaccct atatttgttc attacaccac ttatctcgtc tacaaaggct
     4561 cccagatcgt gtgtaaaggc atcatagtaa tcttcaatct cactcgattc agttctccgg
     4621 tttcttccgt ttttatttct gaatagatac atctgcgttt ctcttaaatc ccttgctttt
     4681 ttcgcaagtg ctaataaatc cgtaaaatta tcaataacca taatgtcatt gatcataaaa
     4741 ccttcttcat gaccatactt ttcagccatc tcgataaaat cattaacatc gaacaactcc
     4801 aataattctg ctttatattt ctctaacaat gtatttaaat agcgatttct caattcccat
     4861 gaactttcac cagttccgta ttcaaccttg atccctaaat tcttagtgta gccttttgct
     4921 gtgtatcgtc caatgaagtc tcccttattc cgattgctat cttcttcttt gaaaaactca
     4981 cgccaaatcc cctttttctt cttaggaatt agacctctgc tcttgaaatc atcatatttt
     5041 tttgaagctt ctcttcgtgt atctaagtac ctttgcttca gctctgtagc tgattcattc
     5101 gttccatatt caacagtgat ccttaagcgc ctgctataac cttttgctgt atatcgtcca
     5161 acaaagtcac cgccacttcg atcactactg acttccttaa caaattctct ccatatacct
     5221 tttttgtcct ttggtattaa tcctcgtctt ttcatatctt gatatttttc agaagcttct
     5281 tttagagtat tcttttttgc taccatatat tccactcact ctttgttatc tagtatttta
     5341 ataactggtt tcatttttgt aatatcttct ttttcagcta tatacaactc cgttacggta
     5401 acgatcttag tatgatttag attaattact ttcacttgat cgccaatttt gatttttcct
     5461 cgcaaccgtt tgggaataaa ccagttgtat aatcgtgatt tattattatc tacataagct
     5521 tttattgcat tttcaggaaa caggttattt agtctttcaa tttttttact tttaaaaaat
     5581 tcattcgcaa acttttcatc aacaactaaa ttaaataaag tttcagaatt tacagcttcc
     5641 atatctattc ctttgtagat aggagaaagc aattttttat attttgccag taatgacttt
     5701 ccagaattac taatcttaat ttggttactt aaatcttctt gataaagaac cccatatcga
     5761 agtaacgcct taatataatg ttttaatatg ccgttgatac cagaataaat atccatgccg
     5821 ttctcaatat agtagaaagt gtcgtctctc caaaacaatt tgaagctatt attcaaataa
     5881 agtaacaaga tcaatatatg tttgtcagaa attctgacaa atctattact ttttagatac
     5941 tcaccaatgg cttcacttaa ctctttgaag ccattgtatt ttcccgataa aaatgtcgta
     6001 acattatctt ctctcttttt cctatggtgt gaacttaccc tgaaataata gagtctattc
     6061 tcatttaata ttgaaaaata aatactttca gttgtttgtg agcattgtat atagttgaaa
     6121 tgatatccat ctggcatgac ttttttaact gtattttgta ctagctctga ccaccttttg
     6181 ttagtagtca tatatttttt tccattaaac caaacataaa tatctttttt aaaaattttg
     6241 ttccccccct attgcgtttt ctttgacatt aaagcccttt gttttcatca cattcgtata
     6301 gccctagcca ctcataacct gatgcttttg cgaagtttat ttaaaagtgt tttcctttgt
     6361 aatctacctt tcccctcgtc ctggtacttt tcttggcgac tattaaacta ttcaactttt
     6421 ttagaatcat tgtcagcttg aaatccttca cgtttcatta tgtcttctgc ttttccaatg
     6481 ctgattcctt tccaatcacg ccatcctacg cttcccttat tttgacgata ggctttaata
     6541 gctgattcgg atatttgcca tcttgtagcc aactctttgg ctgtgtactt atttgaaaaa
     6601 agtagcttct ctatcagtga aatatcaatc attttattct cactcctgaa atcttcatta
     6661 atcatctcta gctagctata tcttcaatca attctaatat gtcggataaa ccatcaacag
     6721 cttcttcaag ttgatcaacg gattcttctg ctttttcata tctttctgat ccctgtagat
     6781 ttttaggtat attttctttg tactcttctt cttgaattac ttctaactca tcctttagct
     6841 cggaaagtcg ctcgcttatt tcttctaacc tttttcttct tctttggttc atcaaaatca
     6901 cctcttcgca taatctctac gattttaata taccactttt gtgtactaca ttcaatattt
     6961 tttgttatag attcgtatta caaaaatttc ctctaaaaaa ataagagcct atttaccatt
     7021 tcagtaatta agctcttatt tcatttagtt gataattcta tcaattaacg tacagcaacc
     7081 gccattccag tcacattaaa tgctctctca tacgtgcttg cattgatcca ttttgcgcca
     7141 aatacagggt caacaacatg gtatgcacca tttctgaagc catccacgag aactacatgt
     7201 ccattccata ctgcacgacc ccaaggcatt tgtttgaact ctggcgtagc atagttataa
     7261 gtaatccaag ttacaaccgg attaccattg cgaatttcat tcttgattcc attcgttcct
     7321 gaaccagtga tattgacggc attaccgcca acgttttttg cccatttaac tactgggtca
     7381 gccatcatgc cttgatacgt tccattaacg ttatgacgcc actctcctga gtaaccatga
     7441 taaggattat tgtcattagc ttttggcata tagtttacaa aactggttaa gctttgattt
     7501 gtggctacac ctttgtagtg taaaccttct aataaagaag ctccttcgca taacattgta
     7561 tttccttgcc atacaacagg aacgttcaac actttttcag tgcttggagc aggtgtagga
     7621 tttggtacgc tgtttggcat tgcataccag cctgtacctt caatcttcca accttgttta
     7681 ctaattacgt tcttttcatt ttgatctaac gtgtagtgat gccaattcac gtatttgtta
     7741 tataaacgtg ttacaggaac tctatttgct ccgttgtagg aatagaacgc aacaccttcg
     7801 tacgtccatc ctgtgtgtga aatcttatct ctttctccgg catcaactgt gtaaaaatgt
     7861 tcaccgttat accgattata caaacgataa acaggagtac cacttgttgg agcttgccat
     7921 gcgacacctt cgtaatgcca tccataacgt ttcagattgt ctctttctcc aacgcttcta
     7981 gtgtatagat gctctccgct attgggatta tacatacgat acatggaaat agagctttct
     8041 gctgaaactg aaggtgataa taatactagc cccattgtga atagaattcc tactaaaaat
     8101 tttttcattt gattttaccc cttttcattt tttattgtca ttattgcatg aaaattattc
     8161 gtaagattgc caattacata aacgattgtt ttaagatcat aatcgttgtt gt
//
LOCUS       DAGCWU010000127         8068 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.127, whole genome shotgun sequence.
ACCESSION   DAGCWU010000127 DAGCWU010000000
VERSION     DAGCWU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.127"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..884
                     /locus_tag="MBZ71_002180"
     CDS             <1..884
                     /locus_tag="MBZ71_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBT4430101.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="MBZ71_002181"
     CDS             932..3061
                     /locus_tag="MBZ71_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBT4430102.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIESVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="MBZ71_002182"
     CDS             3036..3725
                     /locus_tag="MBZ71_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430103.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="MBZ71_002183"
     CDS             complement(4133..4819)
                     /locus_tag="MBZ71_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4430104.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="MBZ71_002184"
     CDS             complement(4955..5149)
                     /locus_tag="MBZ71_002184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430105.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="MBZ71_002185"
     CDS             complement(5199..5639)
                     /locus_tag="MBZ71_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBT4430106.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="MBZ71_002186"
     CDS             complement(5643..6488)
                     /locus_tag="MBZ71_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBT4430107.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="MBZ71_002187"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="MBZ71_002187"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBT4430108.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1514 c         1714 g         2359 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga atcggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccgggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaa
//
LOCUS       DAGCWU010000128         8010 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.128, whole genome shotgun sequence.
ACCESSION   DAGCWU010000128 DAGCWU010000000
VERSION     DAGCWU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.128"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(55..903)
                     /locus_tag="MBZ71_002188"
     CDS             complement(55..903)
                     /locus_tag="MBZ71_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4430109.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1127..3067)
                     /gene="thrS"
                     /locus_tag="MBZ71_002189"
     CDS             complement(1127..3067)
                     /gene="thrS"
                     /locus_tag="MBZ71_002189"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBT4430110.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3404..3583)
                     /locus_tag="MBZ71_002190"
     CDS             complement(3404..3583)
                     /locus_tag="MBZ71_002190"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBT4430111.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3630..5453)
                     /gene="cadA"
                     /locus_tag="MBZ71_002191"
     CDS             complement(3630..5453)
                     /gene="cadA"
                     /locus_tag="MBZ71_002191"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBT4430112.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5731..8010
                     /locus_tag="MBZ71_002192"
     CDS             5731..8010
                     /locus_tag="MBZ71_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4430113.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2347 a         1704 c         1449 g         2510 t
ORIGIN      
        1 tggaaaacta cgttttgtta ttctaaaaaa cttatataaa aaatagtaag gtttttattt
       61 cgaatccata aaatattctt ggattgcttc tcgtgttttt ttcacttttt cgctaagctt
      121 ttcgttttta gccatataga aataaaaaat gatatcgatc gtagcaaatt gggccaaaag
      181 tgaattagtt gccgcacttc gattcgtgat ttcttttggt ctagatgttt gtacgagtac
      241 atcggctatc ttgctaagcg ggttatttcc aaattgcgtc aacgcaatga tttcgatatt
      301 catgcttttc gccagctcgg ctaatgcgac tacatctgca cttctacctg agtttgaaac
      361 aagccaaagc actttctttt tctcattgct gacaagttgc gggagcaaga cgtggatatc
      421 tttttcaaaa atgattggct taccgacacg agaccatttt tgcagaatat cttctactac
      481 taaagaagag gcgccaaccc catataaaaa aacagtctct gccgattcca aacactctac
      541 gactttttgg atcgtttctt cttctagaat atctactgtt tcttttatcg ctacttgtgc
      601 attgttacca agtttgtttt tgatagagta aaaggactca tttgcttcta tatcactaaa
      661 accttcatcg attccagtct gtagttctgt tgaaatcgtg attttgaagt ccgtcagact
      721 ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc gcactcaacc cggaagcttc
      781 tgctaattca ttagctgtca ttgtagggac cttctcaata ttaccgataa tgtagttggc
      841 caactttttt tctgctgaag aataatcttc catcatactt tcaatacgac taattacgct
      901 cataagttcc tccacccttt taatgatagt ctatgtaagt ataccatatc agacccttat
      961 tcacggtcgt atactggata aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc
     1021 catcaatccc acagattcta ttgagcatct gtgtctttga ggaatacctg ttatctattg
     1081 ttcggattgc tttctatcta ctactttttt acaggaaatt tacctattag ttttctctgc
     1141 tgtaattttt gacttctgct tccatagcat caataaagat attcaaatct tctacagctg
     1201 tttctttact tccataacga cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa
     1261 caactaattg gtaagggatc ttttgtgttt gagaggcacg tatcttatag cccatttttt
     1321 cattacgatc atcaacttct acacgcaacc ccttcatttg caaacgttct ttgatttcat
     1381 aagcataatc gccatgtgca tcaacagaaa cagggatgat tgttgcttgt ataggtgcca
     1441 accatgttgg aaaagcgcct ttgtatactt ctgttaaata agcgacaaaa cgttccattg
     1501 tagaaacaat tccgcggtgg ataactactg gacgatgcgt attttccccg tcttctccaa
     1561 catatgtcaa atcaaagcgc tctggcaata agaagtctaa ttgtatagtt gataatgttt
     1621 cttctgtacc aagagctgtt ttgacttgaa catctagttt tggtccgtaa aaggctgcct
     1681 cgccttctgc ttcaaagtaa tctagttcta attcatccat agcggctttc agcattgctt
     1741 gggctttttc ccacattgcg tcatcatcaa aatatttatc tgtattgttt ggatcgcgat
     1801 agctcaagcg gaaacgataa tctgtaatat tgaagtcagc atatactgcc accatcaatt
     1861 caagcgtacg tttaaattca tctttgattt gatcaggacg aacaaatgta tgaccatcat
     1921 tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc acctgatttt tcatagcgat
     1981 gcatcattcc taattcagcg atacgaattg gtaattcacg ataagaatgg atcgtgtttt
     2041 tgtagaccat catgtgatga gggcagttca ttgggcgaag aacaagcatt tcgccatctc
     2101 ccatatccat tggcgggaac atgtcttctt gataatgatc ccagtgtcca gatgttttgt
     2161 acaactcaac atctcccatt attggtgtat atacatgttg gtaacctaga ctaacttctt
     2221 tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc cccttttgga agccagaatg
     2281 gcaaaccaga acctacttct ggggaaacca tgaacaagtc aagttctttt cctagtttac
     2341 gatggtctct ttcttttgct tcttcacgga gacggatata ttctttcaaa gctttcttgt
     2401 caaaaaaggc tgtcccgtat acacgttgca tcatttgatt gtttgagttt ccacgccaat
     2461 aagcacctgc cactgataat aatttaaata cttgtatacg gccagtggat ggaacatgtg
     2521 gtccacgaca caaatcgatg aattcgcctt gttgataagc agtaatcact tcttcttctg
     2581 gcaattcact aatcagttct actttgtacg gatcacttgc aaaaatttct aatgcttcct
     2641 gttttgaaag aacacggcgt tcgatcggca gattttcttt gacgattttc atcatttctg
     2701 cttcgattgc cggcaaatct tctgcagtta cttggttttg tccattatct gtatcgtagt
     2761 aaaaccctga atcgatagct ggtccaacgc caaagtggat atttggatag aggcgacgca
     2821 tggcttgggc catcaaatga gctgctgaat gacggactaa gccaagtgca tcttcatggt
     2881 ctggtgtgat gatctcaata cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt
     2941 ttccgttcac tttaccagct aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa
     3001 ttgccaatgt tgttgttcca gactcaaatt ctttgactgc accatctgga aaagtaattt
     3061 ttatcataat agtttcctcc ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac
     3121 aaaaaaatcc tagcatctct tgaaaagaga tactaggacg aaaagttcgt ggttccacct
     3181 aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa
     3241 caagggtttc gaaagtggtc atcctttggt tttctcctgg aaaagtttca gcctagcttt
     3301 tcctctctta tcgaagactc tcaaattcag ttggctttct catcaatcaa ttatgtgttt
     3361 atttaaccac taataaatag aaaaatcaag tgggaaacta aacttatttt ttccattccc
     3421 catttacacc gtatgttcct tttttcatct cattgatgat gacatggatg tgttcttttg
     3481 gtgcctgcgt gtttttatga acagcttccg tgatatcttt catcatattt tctaattgtt
     3541 ccggactgcg accttctact aattcgacat gtacgaatgg cataatgctc tctcctcttt
     3601 ctgatcgttc ttttttcaac tcgattctat catactatgt ttgtcttggg tactgaatca
     3661 ggaaaaatgg ctggatcttt ttttgctctc aaggcactca aaatagagac cgtatccacg
     3721 acttcttgca aaacagctcc gaataatgcg gggattagtc ccgtacttgc aattaacatc
     3781 aaaaaaatac aaataaagat gccaatcagt acagactgtt tcgctattct catcgtatct
     3841 cttgaaatca ctactgcttt tgctacacgc aagagatcat ctttcaagat gaccgcatct
     3901 gcactttcac tagcagctgt agaaccatga gcccccattg caataccgat atctgcagca
     3961 gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg caacaggtct ttgatcttta
     4021 gggatacttt ctaaaatggt cagtttatct tgaggcaagc tacgggcatg tacagaatct
     4081 aattggactt tttctgcaat cgtttgagca atggcagatc catccccggt gatcatcatc
     4141 aaatttttca catcaagttt tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc
     4201 tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa ttgttacata gattgctgta
     4261 ttggacgttt ctatttcttc agctgaaaca aaagaggcac gtccaatctt tacttcatga
     4321 ttattgacta ctgcttggat tccttgaccg gtcacttctt tcagttgact aacaggatac
     4381 agttggctgt ttttcacgtt gttcactaac gaacgagcta agatatggct agattcctgt
     4441 tctgcactag ctgcatacag aagtagctct tcttttttta ttccattgac tggctgaatc
     4501 gcctctactt ccaatatccc tttggtaagc gttcctgttt tatcaaaagc aatcgttttt
     4561 gtacgtgcga gcttttcaat cgttgttcca gtttttacaa cgatcccgtt tttacttgaa
     4621 cggctcattc ctgcaactaa agcaatgggc gcagctagaa tcagtggaca aggtgatgcc
     4681 actaccagta cctcagcaaa tcgtaaaggg tcttttgaga accaccaaga aataccaccg
     4741 atcaaataag caatcaacgt gaaagggact gcataacgat cagctaaacg gacaaatcgg
     4801 gcaggctttt cttttgattc ttcgactagt ttaataatca tttgatactg gctgtctgcg
     4861 gcacgttttt cagcgatcat ttggatcgat ccgtctccat ttaaagaccc tgacataatc
     4921 tgatctccag gtttcttggt ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc
     4981 gaagattctc ctttggtcac tcgaccatct gcgggtacaa gttcgccagg cttgactagt
     5041 aactggtcat caatatttat ttcatcgatt actatatcct gcaattttcc ttctttgaat
     5101 cgatgggcaa ttcttggggt gttatctaac aaagactgca gttctttacc cgctcgttta
     5161 ttcgcgtaat cctctagact atctccgccc gtaagcatga tcaatatcat caaactagcc
     5221 cagtattctt tgacggctaa tgttgcaagg attgcggtaa tcgctaatac atctacgcca
     5281 tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca tcaagaaggc gagaatccct
     5341 ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat
     5401 aaagctatcc caccaataag caaagtaagt aaaaattttt tgaaatgtgt cattgttttt
     5461 tctcctcttt tatgtgactt agagaggtta gtaataaatt tccagctgcc tctttcatct
     5521 taagcatagt ccttgactag cactttttaa aacaataggc cttaattgaa aacaactatc
     5581 actatcagcg tttccaaaat tcatcagttc tacaaacaaa aatattcagc aaggattact
     5641 ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa ctatgctata cttttaattc
     5701 gttatttacg gcggaaggag caaacatgcg atgaatgaga aacaactaga acaaacgcac
     5761 gtggatgaaa cgatcgaact aatccagatc gaacaaaata tgctggatac aaaacaacaa
     5821 gaattgactc aggaaatgaa tcattcgaca aaagacacag ctaatcacaa gattcgagct
     5881 ggttccaatg aagcttttta tgaatctgct gttgaatatc gccaacatga gcaggaattg
     5941 cttttgaaat accatactct tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg
     6001 aaagatagtc cttattttgc ccgtatcgac tttaaagaag accgtgaaac agaaacatta
     6061 tatctaggta tcgcttcttt aagagatctg gcagaagaaa caatcgttat tgactggcgc
     6121 gcaccaattg ccaatcttta ttatgaaggt gagattggtc ccgcatttta tgaaactgaa
     6181 acagatagaa tagatgtcga tctgcagttg aaacgacaat ttaaaatcca agaaggacgt
     6241 attttatcta tggtagatac gtcggaagta atcaatgatg actttttgct agagatattg
     6301 gatgaatcat caagcaacca tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat
     6361 gcaatcattc gggacacaac aagtaaagta atgctgattg aaggaatcgc cggaagtgga
     6421 aagacatccg ctttgcttca acgtattgct tttctgttgt atcataatcg caagtggctc
     6481 gaggctgaaa acgttcttct attttcaccc aaccatttgt tttctgatta catctcgact
     6541 gtattgcctt cattaggtga aagcggcgtg cctacacaga cattcaaaaa ttatttggtg
     6601 cagctgctgc cagagttttc tttactagat gaacagcaac aagaaagtgg ttttttgcta
     6661 ggggaaaaag atccgataca agtgatgaaa agcggattga ccttagtcga tcagattgat
     6721 cgctatatcc aaggcatcac aaactacggt cccttgttcc gtgatatgaa aataaatggc
     6781 cgtacgatcc tatcaaagga atcgattcgc caatggtata aagaaacaaa tgaacaatta
     6841 cctttacatc atagactttc tctattacaa acgaagctcc tgaaaaaatt aggcggtctg
     6901 caaaaagacg aaacacgtca gcaatgggtc aaagatctgg cggaagaaca gctccaagaa
     6961 ctatacgcaa cagatccaaa tcttgaatat acagaacaaa aggagcgttc attacgtaaa
     7021 aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat
     7081 tatcaatttg tcaatataac gaaacaatat ctccactttc tacaaactgt gccactttct
     7141 cttctaacac gacataaaat cagcgagacc aggtggcagg aacatttgac aactgttcgt
     7201 acacacttgc gtcaaagaga attgcgtcaa gaggatgctg tactattctt tttactgatg
     7261 cgccgaacat atccagtgac ggttacacac aaagctcgct atatctttat tgatgaaatg
     7321 caggatttcg caccatcaca agtggcattg ctgcaagcct tgttcccaaa agcaaatttg
     7381 actttctgtg gagatctaaa tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg
     7441 gaagaccttt tcgtggaccg tgaagtcact cgtttccaat taacgacaag ctatcgctcc
     7501 acaaaagaaa tcactgattt tgctaatcat tttattgtga aagaacatca agtagaaaca
     7561 acagcaagaa aaggagacct tcctttgctc atccaaggaa caacagatga tatcaaattg
     7621 aattggttag cagataccat tcgaacgact cagggaaaag cccggtattg gcgtacagca
     7681 atcatagcta aaacacaaga agaatgcgaa gctctttatg ctcaactgcc agacgagttg
     7741 aaacaacaag tccagctgat tttaaatgag agtgacttta tgaaacgttc cattatttta
     7801 cttccagcct ttttagcaaa aggtctggag tttgaccgag ttttcttgtg ggatgtagat
     7861 gcagaacatt tttcttctca gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg
     7921 atgcatgaat tgaccctttt tacctccaat aatgaaagtc ctttgttaaa caatatggat
     7981 atttcatctt atgagaaaaa agtactgtaa
//
LOCUS       DAGCWU010000129         7917 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.130, whole genome shotgun sequence.
ACCESSION   DAGCWU010000129 DAGCWU010000000
VERSION     DAGCWU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.130"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="MBZ71_002193"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="MBZ71_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBT4430114.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="MBZ71_002194"
     CDS             complement(2203..4389)
                     /locus_tag="MBZ71_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBT4430115.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="MBZ71_002195"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="MBZ71_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBT4430116.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="MBZ71_002196"
     CDS             complement(4611..5051)
                     /locus_tag="MBZ71_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBT4430117.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="MBZ71_002197"
     CDS             complement(5125..5664)
                     /locus_tag="MBZ71_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBT4430118.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="MBZ71_002198"
     CDS             complement(6279..7745)
                     /locus_tag="MBZ71_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4430119.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2150 a         1737 c         1375 g         2655 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaact tacaagacag tgttatttta tgtgtgtatt cttttattta atacacatcg
     7861 ttttttcata ttttttccat caaaaaaata agttattttc ttttatttca tttacct
//
LOCUS       DAGCWU010000130         7637 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.131, whole genome shotgun sequence.
ACCESSION   DAGCWU010000130 DAGCWU010000000
VERSION     DAGCWU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.131"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            371..3178
                     /locus_tag="MBZ71_002199"
     CDS             371..3178
                     /locus_tag="MBZ71_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4430120.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLGTFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3369..3860
                     /locus_tag="MBZ71_002200"
     CDS             3369..3860
                     /locus_tag="MBZ71_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4430121.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3963..4952
                     /gene="manX"
                     /locus_tag="MBZ71_002201"
     CDS             3963..4952
                     /gene="manX"
                     /locus_tag="MBZ71_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBT4430122.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4985..5788
                     /locus_tag="MBZ71_002202"
     CDS             4985..5788
                     /locus_tag="MBZ71_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBT4430123.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5809..6723
                     /locus_tag="MBZ71_002203"
     CDS             5809..6723
                     /locus_tag="MBZ71_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBT4430124.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6806..7180
                     /locus_tag="MBZ71_002204"
     CDS             6806..7180
                     /locus_tag="MBZ71_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBT4430125.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7319..7564
                     /locus_tag="MBZ71_002205"
     CDS             7319..7564
                     /locus_tag="MBZ71_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4430126.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         2475 a         1263 c         1683 g         2216 t
ORIGIN      
        1 ataaaagttg tagtggaggc tacgaagttt actttgtctt tttacactta atattttttt
       61 gccaatttgg aatcaggatg ttttgataac tatcgttttc aattgatgga agctatattg
      121 tttgctatca gtgaaataat tatatggtaa acagtgttct ttttcttgtt tccattataa
      181 aaatcgtatc gggatttttc gttcttttaa attgaacatt tctatctatc cgcgtgttat
      241 caacaaaaca aaatcaggta aatagttgac gaaacagaaa taaatacata gtattatgtt
      301 ttgaagccta agtacaatag tctatagaaa aggagcgata ccattggcaa aacgcattga
      361 ccgaatttat atgtatgtca aagaaaaaac cgctcattta acaaaggcag aatatgatca
      421 aggagtaaca acacaggaaa ttgctgaagt gttagggatt cagcgaacaa atagcagtaa
      481 agatctgaat cagctagtgc gcgaaggaaa actgataaag acagacggac gaccagtacg
      541 ttacatatat caaacactcg ttacgcacgt aaagccagtt acaaaacatg tagtcagtta
      601 caaagaaacg ccggtctctg aagaaaaaaa tacactccaa atagatacta aggatatctt
      661 tgcaaaaatc attggagcta acggcagcat gaaaaattca gtggaacagg caaaagctgc
      721 gattttgtat ccgccaagag gattaaattg tttgattaca ggacctaccg gatcaggaaa
      781 gacttatttt gcacacgcga tgtatcattt tgcaaaagcg catcatgtaa tagatgagga
      841 aacggaactg attgttttca attgtgctga ttatgccaat aaccctgagc ttttgatgag
      901 tcatttattt gggtacgcaa aaggtgcttt tacaggcgca gatgaagaaa aaatggggat
      961 tatcgatcaa gcggatggcg gtatgctgtt tttggatgag attcaccggt tgccgcctga
     1021 aggtcaagaa atgatctttt actttatgga tcatggaact tacagtcgtc ttggggaaac
     1081 aacgaaatcc catgaagcaa atgtacggat cgtaggtgcg accacagaag acccaggttc
     1141 ttctctatta ggaacttttg ttcggcgtat cccaatcaat atcaaacttc cggcttttga
     1201 aaaacgtcct gcaaatgaga aagtcgattt agtaaaaatc atgattgccc acgaagcaaa
     1261 ccgcacgcag cgaaagattt ctttgacaga agatgtagca aaagcattga tcggcagtgt
     1321 gacctatgga aatataggac aattgaagtc aaatatccag ttagtatgtg cacgaggatt
     1381 tctaaatcat atgcaatcac ccgaaatctc gatcacaatc gatgatttga cagaagggat
     1441 tcgtagcgga ttgattcaat tagcaagcaa ccgcagtgca atggcagagc tttctaaaat
     1501 attggagcca aagataactg tagcaccaaa tgatacgatc atgaaaatcc aatcggactc
     1561 ctatgagtta ccttataatc tatatgatat catcggagat aaagcagctt tactgaagtc
     1621 ggatggtttg gatcaagaag caatcaatca tttcatttct acagatatca acatccatct
     1681 gaagtctttc tataaagacc atggtttttc ttttaatgca gacagcaaat tagcagaatt
     1741 tgtagatccg aaaatcatag aagtgaccaa tcagatttat gtaatggtca aaagatcttt
     1801 accatatgag tttcagcaaa actttattta tgctatgagc ttgcatatca gttctttttt
     1861 gaaaaaaatc aacttaggag aagaacggca tacgaatgat aacatccgtg agatggcttt
     1921 tgattttcca gatgaatatg ctgttgcgaa agaagtgcgt agttatatcg aaacgtattt
     1981 tcaagtggag attccagaaa gtgaagacta ttacttgacc gttctactcg tctcgttacg
     2041 ggcaaatcaa gcaagcggca gaatcggtgt agtcgttgca gctcacggaa acagcacagc
     2101 aagcagtatg gtccaagttg tccaacagtt gttagatgca gagaacgtac gagcagtaga
     2161 catgcctttg gatatggatc cgaaaacagc attggaccga attgaacgca atgtacaaga
     2221 agtagatgaa ggaagcgggg tcatactctt agtagatatg gggtcccttg cgtcgtttaa
     2281 ttcgcaaatt caaagagaca ctggaattcc agtacgtact gttgatatgg taacaacttc
     2341 tctagttttg gaaactgtac gaaaggtctc agtattaggg acagatctag atatgttgca
     2401 tgaatcattg aaaaattttc gaggttacgc agaaatatct tctgtagaat ctgttcagga
     2461 agcaaaacct attcataaga aaaaagctgt tttggctatc tgcgcatctg gcgaaggtac
     2521 agcacaaaga atcaaagaat tgatcgaacg agcggtaaac aagcgtcaag aaacagaatt
     2581 agaagttcta gctttgtcga ttgtggaagt gaaagaagaa ctgccagtta tccaaaaaga
     2641 atatcagatc atagctacta ctgggattac tgatttgaat atccaagccc catttattcc
     2701 tttagaacgt tttattgatc aaaatatcga agtcattttg gatcagttgc tgatggaatc
     2761 agaattggag gaaactgaat tcatctccct agatgaagag tcagctaaaa atacgtgtgt
     2821 tgagtttatc tctgataact tcatctttat taatggtagt aagttaatcg atccaatgtg
     2881 gcaattttct acacaaatct ctcagacgac gggaatcggc gatgaagaat atggattcaa
     2941 aatcaaccta gtgatgcaca cagcaggaat gatcgaacgt attattcgga atgaaccatt
     3001 gactgtggaa gaaaacgaat tgacaaatac aacgaatgat cctctttata gccaattggc
     3061 agcctcagtt gttctgttgg aagatcagat aaaagtaaaa gtgccgatag aagaaatgta
     3121 ttatctgcta cgtctagtcc ataatcagtt agataaaaaa gaatacactg tgccatgaat
     3181 acacaaaaac gatacacttt tatagtgtat cgtttttgtg tattggttaa gaatccttta
     3241 aattcaatta gtgtatcgta aaaaagttgg cacgcttttt gcttatagta gtgtgtgcta
     3301 ggaggtggtt gttcacacag aaatgatagg ggtttctgtg aagtagtgag gaagaaggag
     3361 ttttgcagat ggatattcga ttggcacgta tagatgatcg tttaatccac gggcaagtcg
     3421 caacagtttg gtcgaagcaa acaggtgttg aacgtatcat tgtgatcagc gatgtagtcg
     3481 ctcaagataa attgagaaag tttctactaa aagaagctgc accgccgggg atcacagcaa
     3541 atgtaataac cgtgcagaaa atgattcaaa tctatcatag tggattatta tcacgagcaa
     3601 aagtgatgct tttattcacc aatccccaag atgttgaagc ggttgtccgc tgcggtgtac
     3661 agttgaagtc gttgaatatc ggcggtatga gttttactga aggcaaatca atgattacca
     3721 attttgtttc agtaaatcaa caagatattg attcctttcg ttacttgaac agtcaagaag
     3781 tggagttaga gattcgaaaa gtacctgctg acaggaaaat ccttttgatg gaactactag
     3841 cgaaaatcaa aaaggtgtag caagaatggt tgttggaaaa ggtcaaataa ttctgttgta
     3901 atggttagca attattaata tttttgaatt tgttacaaca aaagtaaata ggaggtataa
     3961 atatggtagg gattatccta gcaagtcatg gacaatttgc tgaaggaatc ttacaatccg
     4021 gttcaatgat ctttggcgaa caagaaaacg tcaaagccgt gattttgaaa ccaagtgaag
     4081 gtccagatga cttgagagcc aaattggaag aagcggttgc ttcttttgat aatcaagacg
     4141 aagtgttatt cttagttgat ttatggggcg gaacaccgtt caatcaatca aacacattat
     4201 tcgaagagca caaagataaa tgggcaatcg tcagcggttt gaatttacca atgttgattg
     4261 aagcctacgc atcacgcttt tcaatggaat ctgctcatga aattgcagca cacatcattg
     4321 aaacagcaaa agacggtgta aaagtaaaac cggaagaatt agaaccggca gaagcaccga
     4381 aagcagcagt tgaagacgca caacctaaag gagcacttcc agaaggaact gtggtcggag
     4441 acggaaaaat caaatatgta ttagcacgtg ttgactcacg tctgttacat ggacaagttg
     4501 cgacagcttg gacaaaagca gtgcaaccaa accgtattat cgttgtttca gatgcagtat
     4561 caaaggatga tctgcgtaag agactaatcg aacaagctgc tcctccaggt gtaaaagcaa
     4621 atgttattcc tattagcaaa atgatcgaag tagcaaaaga ccctcgtttt ggaaatacca
     4681 aagcattgct tttatttgaa aatcctgaag acgtattgac agctgttgaa ggtggcgtag
     4741 acatcaaaga attaaatgtt gggtcaatgg cgcattcggt cggtaaagtc gttgtaagta
     4801 aagtattgtc tatgggacaa gaagacgttg aagcgtttga gaagcttgaa caaaaaggtg
     4861 tgaaattcga tgtacgtaaa gtacctaatg attcacgcga taacatggat gatattttga
     4921 aaaaagcaaa agctgaatta gcaaaagcgt aataaataat attaaaattt ataggaggct
     4981 tatcatgtct attatttcaa taattttagt cgtattagtt gcctttctag ctggtatgga
     5041 aggtattctt gatgaatttc aatttcatca accactagtg gcatgtacat tgatcggttt
     5101 agttaccggt aacctcgaag ctggtatcgt acttggcggt acgcttcaga tgatcgcttt
     5161 aggttgggca aatatcggtg ccgctgtagc accagatgct gcattagcat cagtagcatc
     5221 agcaattatt ttagttttag gtggacaagg tgttaaaggt gttccttctg cgatcgcgat
     5281 tgccgttcct ttggctgttg cagggttgtt cttaacaatg atcgtacgta caatcgctgt
     5341 tccaatcgtt catttgatgg atgcggctgc tgaagaagga aacatccgta aggttgaaat
     5401 gtggcacatc attgctgtat gtttacaagg ggttcgtatc gcggtcccag cagcagcgtt
     5461 gctatttatc ccagcacaaa ctgtgcaatc attccttgaa tcaatgccag catggttaac
     5521 agatggtatg gctatcggtg ggggaatggt cgtagctgta ggttacgctt tggtaatcaa
     5581 catgatggct acaaaagaag tatggccatt cttcgttatt ggtttcgttg tagctgcaat
     5641 ttctcaatta acattgattg ctctaggtgc tttaggtgtt gcattagctc ttatttactt
     5701 gaacctttct aaaatgggtg gttcatcaaa taatggaggc ggaagcaact ctggtgaccc
     5761 tttaggcgac attttgaatg actattaatc ttgaaggagg aaaataaaat ggcagaagaa
     5821 aaaatcaaat tatcaaaaaa agatcgttta gctgttgcgt ggcgctctac cttcattcaa
     5881 ggttcatgga actacgaacg tatgcaaaac ggtggctggg cattttctat gattccagca
     5941 atcaaaaaat tatacaaaac aaaagaagaa cgttctgcag cattaaaacg tcacttggaa
     6001 ttctttaata cacacccata cattgcttct ccaattttag gggttacatt ggctctggaa
     6061 gaagaacgtg cgaatggcgc gcctgttgat gatgtagcga ttcaaggggt taaagtaggt
     6121 atgatgggtc ctttagctgg tgttggtgac ccagtgttct ggtttacagt acgtccaatg
     6181 ctaggtgcac taggtgcttc attagctatg ggaggaaata tccttggacc aatcatcttc
     6241 ttcttaggtt ggaacattat tcgttgggca ttcatgtggt atacacaaga atttggctac
     6301 aaagctggtt ctaaaattac tgatgacctt tctggcggat tgttacaaga cgtaacaaaa
     6361 ggtgcttcaa ttttagggat gttcgtatta gctgcccttg tacaaagatg ggtatcaatc
     6421 aaattcttac cagttgtatc aactgtaaaa ttagataaag gtgcttacat cgaatgggat
     6481 aaattgcctt caggtggtga aggaatcaag agtgcatttg aacaagttaa taatggctta
     6541 gccctttctc ctgaaaaggt aacaacatta caagacaact tagaccaatt gattcctggt
     6601 ttagctgcat taggattaac attgttatgt atgtggttgc ttaagaaaaa ggtttctcca
     6661 atcgttatca tccttggttt attcgtagtc ggtgttgttg gacacgtcat cggactttta
     6721 taatccataa aaatatagct cgggcgttgt cccgagcttt ttttatatag tatactgttc
     6781 ataaacaaat atctggaggg agaaaatggt tcaatcaatc aatacaaaag tagacttggt
     6841 aattgatgct acttcgttta cgggtctgac agattacggg caaatcatga taggtgataa
     6901 tggttttgag ttttaccatg cgcgagatag acgcaaatat atccaaattc catgggaaga
     6961 agtggattat gtactagcat cagtcatgct aaagggaaaa tggattcctc gatatgccat
     7021 tcaaacgaaa aaaaatggta catacacatt ttcttccaaa gaacctaaaa aagtgcttcg
     7081 tgcgatcaga gaatatgtag atgaagatag aatggtacaa tcattgagtt tctttgatgt
     7141 agtgaaacgt ggtattcgta gtatgtttaa acgcaagtaa aaaataaaga aaacttctaa
     7201 gaggaggttt tctttatttt tttactttgc agaagaaaat atgaaatagc aaaaccaatt
     7261 atttgtcccc tttaaatccc atatgctaaa attgattagc aaagaaggag gttatattat
     7321 gagttggtta tggacattga ttgttggagc cgtgatcgga gcaattgcag gtgcgatcac
     7381 cagtaaagga aattcgatgg gctgtgtaac gaatatcatt gctggactag tcggttcaag
     7441 tattggacaa gcgattttcg gcgaatgggg accaaaagct gcaggtatgg cacttattcc
     7501 atctatttta ggcgcagtga ttttggtagc agttgtctca tttttctttg gaaaacgatc
     7561 ataagttttt acaaaatagt aaactaaaag gagttgcagc gagactgtga ctcctttttg
     7621 gctttattta tttaaaa
//
LOCUS       DAGCWU010000131         7622 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.132, whole genome shotgun sequence.
ACCESSION   DAGCWU010000131 DAGCWU010000000
VERSION     DAGCWU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.132"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(12..704)
                     /locus_tag="MBZ71_002206"
     CDS             complement(12..704)
                     /locus_tag="MBZ71_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4430127.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1141..1710)
                     /locus_tag="MBZ71_002207"
     CDS             complement(1141..1710)
                     /locus_tag="MBZ71_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4430128.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1784..2107)
                     /locus_tag="MBZ71_002208"
     CDS             complement(1784..2107)
                     /locus_tag="MBZ71_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430129.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2261..3073)
                     /locus_tag="MBZ71_002209"
     CDS             complement(2261..3073)
                     /locus_tag="MBZ71_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430130.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3427..4275
                     /locus_tag="MBZ71_002210"
     CDS             3427..4275
                     /locus_tag="MBZ71_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBT4430131.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4420..5361
                     /locus_tag="MBZ71_002211"
     CDS             4420..5361
                     /locus_tag="MBZ71_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBT4430132.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5351..7522
                     /locus_tag="MBZ71_002212"
                     /pseudo
     CDS             5351..7522
                     /locus_tag="MBZ71_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2271 a         1512 c         1370 g         2469 t
ORIGIN      
        1 ggggggattt tttatcgttg tggaatattt aaataccaac gcttgtcatg aaaatcttgt
       61 gctccaccct tagtatttcc ttctggatcg tttgtcgcac gcatcatgac gtatactttc
      121 ttattaggga aatcacgcat attgaaagat acatgatagc caacatttcc taaagtatta
      181 taagcttgat ttacgtctgg tctataaatc ccatcagctc ttactctagc tagttctttt
      241 ccagtattgt agtccataat gaaaatatac tcgtatttat aattagcaat gtgccatcca
      301 gctacatgca agtttgcgtt ttcgatttct ccgaactgat caatgtgggc gtaatttgtt
      361 ccatctgtca gcgtagaatt tgcagcacct gctcgagttg gatcaattac tggtttatcc
      421 tccgaagtag ttggattatc atccgtaaaa ccatgagcca aatcataggc tagtttttct
      481 ttacttacac ctatttcaga aagataaccg taaggatctg tatgatcgcc ccaaatattt
      541 tgtgttaccc ataaatgcga tttgattcct ggttggttat aaggagtgtc taatgttaat
      601 ggaataccat atttcattgc tgaatctctt gccaattcaa cgtatacttt atagttcttt
      661 tcaaaagttt ctttatcatg tgtgtgttgt aactcgattt gcacaggact tttggctcac
      721 gaggttgtcc tactctacat cttgctgata gatatgctga aaggaaaaca ccaacattat
      781 gttttgcttt ttcagctaga acgagacaag caggaatacc tttagaagat atcaaaggtt
      841 ttttaggtca taaagatgtt tccactacac aagtctatgc ccatatttct cccgaagtga
      901 aaaaaagatc aatgaatcaa ctagaaaact atatagaaga gcaaataaaa aagcactcaa
      961 attgagtgct tttttaagct cactatcact aaaactatca ctaattttat ttataagcga
     1021 acatgtttct atcactgatt ttttaaatct agcgaaaaac ccttatctta aaggcgttcg
     1081 ttcaattctt tggcaaggtc ttcaaagcct ggcttgccta gaagtgcgaa catggttttc
     1141 ttatagctca tgtagaaaaa ttttcttgat gatgatttta tccatcaatt gagattgact
     1201 gttttgctct cctatgattt cagctatgat tgtccgattt tgcggttttc ttcttaactg
     1261 gtagttgatt ttcccagttt ccatcaactc gctaagcata tccacgactc cgccaaatcc
     1321 ggtctttttc gtaataaaac ctagtgctac cattcgttca tctagatttt ttgctgtaat
     1381 cgtcacacca gtaatgagcg agtgctgtaa tgtttgggcc agctcttcca tcccttcagc
     1441 attggatacg acggaagctg ttttgttaat aggttcttcg tttatttctt ttggttcttt
     1501 cctctccact ttcactgttt gacgaaggac atctagttct tgcaagagcg tttcgtattg
     1561 ttcttgggtc agcatcacgc cagctacttt ttcacgatta aatatatata cgccagttcc
     1621 cgcgtctcgc gcttgagcaa atacatccat cggcgatcgt ttcacttctg tgatcgaaga
     1681 tgtgggaact tctaatttct ttaattccaa tggatttcct cctttactaa tcgcttttta
     1741 ttatagcaca gactgacttg ttttgatctt ttttccaaag gttttatcgg tttttgccta
     1801 accagtaatt gatatcggta agaagtcttt cgataccaaa tgaaggatgg tacagatacg
     1861 ttttttgatc ttcgttatgg tctagtggaa aaaaatgatc gatgatcact agatctgcat
     1921 accgaggcgg cttatttcga gtacatacat gtatagagat ccgttttcga tgtcttagcg
     1981 ttttgttcag taccccagca agtcttcttg cttcttggtg ttcatagcga aaaaaaaaga
     2041 cgattttcac ttcaggaggg tggacatcta aataccctcg ttcctggagt gttttcagta
     2101 aaagcatccc ccactctact gcggggtgtg caagtgcttg ttggatcgct agccgaaatt
     2161 gatatagttt atctttggaa ttgggaaatt tttctaatgt ttcctgataa taacgaactt
     2221 cttctatcag ccggtcatgg ataccaatta cgtaacgaag tcattcttgg taaagtactt
     2281 ttgtcagctt tgatttgctt tcgctctcaa attcgctatt cagccaaaac agcgaatcaa
     2341 gcttttcagt cacaatcgtc aatttccgat agtcttgtgt ctcttctatc cgtttccacc
     2401 gcttttcagt aatctggtta cagctcaagc gcaaaattgc tagataattt ttttctttta
     2461 ttggcaaaat ctgctcctct ctaaatagca attcaggaaa caattcaaag aatagtgttc
     2521 gactgtctcc taaaatctcg atttctgccg gatcctttct ttgcttcaat aattggatga
     2581 acatctcaga aaaggactgc tctccatcat ctgcaaaaaa atgcggaaaa tctttcaaaa
     2641 agcaaaacag cagatatctt acttttcttt ctgaaccaaa gatctgtgct tttttataag
     2701 aaaaagaaag gtggtactcg ccaaaccact ttctcaatcg ctcttgcgta gaacgatcgt
     2761 acttctgctc gtaaggttca ttcaatagca gatttttcag taaacaaaac gggggtgatt
     2821 ggatcagaaa aacaaacaag agataaggtt cgctatatcc ttgatcataa actaactgaa
     2881 cggttttctt atgaagaatc agctgaatgg gacagtcgct ttccgccaaa cgtttcagat
     2941 gccgatccac tgttttaaca tcgattttca gttcttcagc taaatcagct tttcgccaga
     3001 tcaaatcttt cattagattg attagtgaaa ataaattctg tgcatttttt gataatagtt
     3061 tttgccatct catcgatcat ttctcacctg atttctaaac atattgtttg atcaaaatgg
     3121 gttcactcct aaaaagataa aaaactccct ttctatttat ttaaatacgc aaatcttcct
     3181 cattattttt tattttactc aatttaatcg tattctcatt tcaaaacgaa tgtactttaa
     3241 aaaaacataa taagatcact ttgcttgtag gatgtattcc cttccattac aatgaaataa
     3301 atagcgattt tatgagaagg tgatgtgaaa atggaagaaa aaatagaaga atccaaacaa
     3361 aaaatcgatc agttgatata agaattagaa aaagcggtca tcggcaaacg ttcaactatc
     3421 caattgatga tcgtggccct attagcaggt ggtcatgtct tattcgaaga tattcccggt
     3481 gtaggtaaaa cgttactggt caaagcacta gcaaaagctg tacagggaac cttcagcaga
     3541 gtccaatgta ctcctgatct gctgcctagt gatattcttg gtttttccgt atacaattca
     3601 caatcaaaag agtttgaatt tcgtccggga ccgatcttta ccacgatctt gttagcagat
     3661 gagatcaatc gtacgactcc tcgtactcag tcagcacttt tagaagcaat ggccgagaat
     3721 catgcaacga tcgacaacaa tacataccct ctagacaatc atttttttgt cttagctacg
     3781 caaaatccaa tcgagtacga aggaacatat cctttgccgg aagcacagct cgatcgtttt
     3841 ttattccgac tacaaatcgg ttatccttct tttgatgacg aacttttgct acttttggat
     3901 aaattcgaaa aagtgttgga taacttaaat gaagtattga acagttatga aatagaagaa
     3961 ataaaaacaa atgtggcgaa tgtctttgtc agtcccgaag tggcatctta tgccttgcag
     4021 ttagtgtcag caacaagaag tcatgctgcc atccagcttg gcatcagtcc acgaggaagc
     4081 ctttccttta ttcaggcagc aaaagcttat gctttaatcc atgggagaaa ttacgtcacg
     4141 cccaaagatt tgcaggattt agttccttac gtgttttccc atcgcctgat tttttacgat
     4201 cgttcattga aggaagacga aaagaaacaa atcattcata ctcttgttgc acaaattcct
     4261 attcctgtga ggtagattga ggtagacaat ggaaaaaatc aaagattatt tcattaattt
     4321 tgtgttattt ctggtatatt tcatcattgt gctttttgct cttgtattca acaattcttt
     4381 aggctggttc ttgttggcct tttttactct ttatgtatca tggagcttga tttcattaag
     4441 cagctttcta aagaaacttt cggttacttg tacattgcag ccactcgttt atcgcaaaaa
     4501 aagtatgcag gttatttttt ccatagcaaa aaagagaact ttttctttac ctctccctcg
     4561 tttacaaatc ggcttcccag caatctttgc agaaaaaaac aagagatttt gattcttaca
     4621 aataaaccgc aagaagtcca attgctttgg cagccaaaag aaagaggatg ccatgaaaca
     4681 ttaactgtca tttttacagc ctatgatagt ctaggacttt tcagaaaaag tgcaaaaaga
     4741 gagatctctt ttcctgtgac tgttctgcct gctttttgta agattcaagc agaaaaatta
     4801 cagagagttc tggaaaaaaa acagcagctg aacgcgtacc aaagaggtct tgattttcgg
     4861 gaacatcgcg cttatcgtcc aggtgattct ttcaaccgaa tcgattggaa attgtctgct
     4921 catactcaag agtggttata ccgtgaatac gattatcaag aagaagaatg ttctcctctt
     4981 ttttgctttt ggggttcgcc cagtccaaaa tttgaacctc tgctagactt gtatttttct
     5041 ttatggcatt tgagaaaaga aaagcagccg gaacttctga tattaggcaa ccgcgccttt
     5101 catggcaaag accccggtca tacctatttt gcttctttgg aagctggcga tgagaaaact
     5161 tttttcgcac acttgaaaaa atatgcaaaa aaacaaatcg tcctctttgt tcctgagaat
     5221 tctcctgaag tctcagaagc tattgaaaca ttgtcaaaag atcgaacggt ttatcttgtt
     5281 tatttcgtag aaaaacaact catggtccaa gaaaaagaca agatatgttc attgccagga
     5341 ggtgaatgga ttgacgatta aacgggaaaa atggctgcgt ggcgtttact cctttgtgat
     5401 actaagtgta ggtgctccga tatttgtagt agctaatcaa ttgcaaaaca gctaccttct
     5461 tatcctaact attggtgtcc tatgccttat tgtctgctgt attcctaaaa aatgggtacg
     5521 tttgctgctt tctttgccag taatgatagg ttgtttgttt ttatattttc cttcgaagac
     5581 attttccata ttatggatct tacagttttt ttcgtcagtt acagaggaat tcttccatct
     5641 atcccaaggt aaactttatt atttgccaga aaagacagct ttttttctca taatgttgct
     5701 gatctattta tttattacat tgaccgtgga ttacgactat tggtacgcac cctttctttc
     5761 tttgatggct tacttcatga tgcttaccgt ttttcggaaa aaagatttac tgtacgaaat
     5821 gattgcggta gtaactgttt tgttcgtgta tctggcagca aggcaatttt tctccatccg
     5881 gttgttgtcg agaaacactc gattccagtc attgactaca gttctgctta tttgtttctt
     5941 aacttttagc gctatcttgc ctttatatct tccgaaagta caccaatctc tcgaggaaac
     6001 gagtgaaccg atccgagaat atcttaatga tgaagggctt tatgacacac tccatgagta
     6061 tcaattaacc ggaagtgcaa gaacaggctt cagtgagaat gatgaacagc ttggtggacc
     6121 tttatatgat aatggcgaag ttgtttttac agcgaaccaa aaaggaaatg gctattggaa
     6181 gatcgaaaat aaaaatcatt acaccggttc tggatgaata gaagatcagg acgcacccat
     6241 ccaaccaatc caagaattcc ctgttgacat cacgagctac caaaccgtta tagaagacaa
     6301 agaaaacatt acattgaccc taaaaaaaca agctttctta ccttatcctt atggaaatac
     6361 taccttgact gatttttcat tagctagtgg acaaacgctt tactatttgc ctgaaagcca
     6421 gcgattcatt cctgtcccga atgaatcgac acgttctgca actttacaaa cagaaaggtt
     6481 agtttttacg agagaagaat taaaaagcag tcagttaaaa gctgatgatt tagaaaatca
     6541 gcagacaaat cttcaattgc caaaaagttt gcctaaacgt attgaggact tggcggtcga
     6601 attgaccaaa aatgaagcaa ctttaattcg taaagtcgaa gctgtctaac agtatttaaa
     6661 aacaagcgga aatttccgct attccaaaac ggataccggg catactccaa aagaacacga
     6721 ttatgtggat tatttcctct ttgattcaaa agtcggctat tgtgacaact tttcaacttc
     6781 tatggtcgtc cttcttcgaa cacttggtat cccagctcgc tggacaaaag gttttacaaa
     6841 tgggctcaga acaggcacag ataatcaagg gaatgaggaa tacagcattc tcaatagtca
     6901 tgctcattct tggccagagg tatactttcc tggatttggc tggcttccat ttgaaccaac
     6961 cccttcattt gccaatccaa tgggttcaca accagttgct gaaacaaccg aaaccgtcca
     7021 gcctgctatc gaacaaccaa atgaaagcga aaatatggca gaaactattt ccagtgagag
     7081 atctgaggtc attccatcag aagaaacaac actcacacca gcttataaaa tcgtcttatt
     7141 cagccttgga gtgtgtttat ccgtcttgtt gttctttcta ttgttattca gaaaatctct
     7201 gcgctggaca agttatctta tctatcttca tttttttgct tctactaata cgattgcata
     7261 tcaaaaactt cttaaccctt tggaaacatt ggtaccgcgc aaacccgcag aatctttgca
     7321 ggaatatagc ctgcgaatag aacaagaatt caagctagat ctgcgtttca ttcgattaac
     7381 tactcaatat gaagcagaaa tatatggacg acaagcagat ccggtaaaat tcgataaaaa
     7441 aacctattta gaaataacgc atataataag aaaaacggta tacaaaaaaa ttctgacaaa
     7501 agggagccgc ttcaaaaaat aagataaaat ttctggagaa tctacttctc gaatgccgag
     7561 aaccagctga ttgagtatcg attactcgaa aaaaaggttg tgacacgttt tttgtcacaa
     7621 cc
//
LOCUS       DAGCWU010000132         7472 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.133, whole genome shotgun sequence.
ACCESSION   DAGCWU010000132 DAGCWU010000000
VERSION     DAGCWU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.133"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            75..854
                     /locus_tag="MBZ71_002213"
     CDS             75..854
                     /locus_tag="MBZ71_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430133.1"
                     /translation="MEKPFVKSLTAVAVGAGAIVICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVIQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNISY"
     gene            900..1844
                     /locus_tag="MBZ71_002214"
     CDS             900..1844
                     /locus_tag="MBZ71_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBT4430134.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGSDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1860..2639
                     /locus_tag="MBZ71_002215"
     CDS             1860..2639
                     /locus_tag="MBZ71_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBT4430135.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKRTAPARPKATNDKTAPSRRSRSRV"
     gene            2651..3349
                     /locus_tag="MBZ71_002216"
     CDS             2651..3349
                     /locus_tag="MBZ71_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBT4430136.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3377..4141
                     /locus_tag="MBZ71_002217"
     CDS             3377..4141
                     /locus_tag="MBZ71_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBT4430137.1"
                     /translation="MIDLHCHILPGIDDGSENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4155..5974
                     /locus_tag="MBZ71_002218"
                     /pseudo
     CDS             4155..5974
                     /locus_tag="MBZ71_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            5985..6617
                     /locus_tag="MBZ71_002219"
     CDS             5985..6617
                     /locus_tag="MBZ71_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBT4430138.1"
                     /translation="MKAIIIGAGGFGKEIAFLLQSVSRYELIGFVDDSLKMKNQELLG
                     KPVLGTIDSLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDAQIGNYNSIFPSVNISGHVILGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            6619..>7472
                     /locus_tag="MBZ71_002220"
     CDS             6619..>7472
                     /locus_tag="MBZ71_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBT4430139.1"
                     /translation="MENKRILLASPHMSEEGYEQEYVKEAFDTNWVAPLGANVNGFEK
                     ELAKYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKNELSKLSNIQF
                     MPENNWNEP"
BASE COUNT         2636 a         1287 c         1556 g         1993 t
ORIGIN      
        1 aataaaaagc tgtgattttt atgtaagtga attatatctt aatttatcta tcatcaatac
       61 ttaggagtga atctatggaa aagccattcg tcaagtcatt gactgctgtg gcggtaggtg
      121 caggtgctat tgtgatctgt ttgtttggtt accatatcaa taatcagcgg caacatcagc
      181 aacggatcaa ttatgcagaa tctgccatca ccaatcaaaa agacaccttg acttctttat
      241 cgaaagaagt agacaagctg tatagtacaa aagaaaaaat ttttctaaac cctgaaataa
      301 cagaagaaac aattacaagt ttgtccaata agctatcctc tgtcaaactt tcagcagatg
      361 attacgacat caaagaatca gaattgccta aagaagctgt tgcaatccaa gatgagaaga
      421 aagcaatcgt gacacagtta gatagtgcag aatcaaaact gaagatccag acagctgtca
      481 acgatttatt tactaaaaat gtatcaaact ggcagcaagc tgtagacgat gtcatcatca
      541 aagaaaaggt gacaagtgca gatgtcgctc gtgtacgtga aaatatgagc ttttttaaag
      601 atagtccttg gaaaacggtt gttatacaat atctaggatt cgctgatacc cagatcgcgc
      661 aagtcacgca actagatcag ttattcgata cgatgttaaa ggatggtcaa gtgactgcca
      721 ccgctactta cgatcaatat ttgacggcgc tcagtcaaat agagcagatc cggaatgaaa
      781 aaataagcgt agcatacgca acaaaagcac aaactgttgc tcagcaaatg ggctatagca
      841 atataagcta ttgacagtta aagcgttgat tcaagcaagg ttagcaaacg gaggaaacta
      901 tggcaagacg aagaagaaaa tcaagaggga aaaaaatatt tctgattatt ttagcagttc
      961 ttatcgtatt agtattagca attgtaggag taggagcaaa attatatctg gatctctcac
     1021 attctattga gaaaacttac gaatcagtgg aacgaaccca aggagaacga acgaaaccag
     1081 ttgatctgga caaacaagaa tctttctctg ttctattgat ggggattgat actggagatt
     1141 taggtcgggt cgatcaagga agatcagata cgatgatggt tgccacagtc agtcctgaag
     1201 acaaacagac aacgatcgtc agcattcctc gagatacgta tgtcgacatc gtaggtagtg
     1261 acacaaaaga caagatcaac cacgcttacg cattcggcgg agctgccatg gcgatggata
     1321 ccgtacaaaa atatgtagac atcccaatcg atcactatgt ctctatcaat atgaaagggc
     1381 taaaagaact tgttgatgca gttggtggta tcgaagtcaa taatgacctc actttctcac
     1441 aagatggcta tgactttaca atcggtaaaa ttactttgaa cggggatcag gcattatctt
     1501 attctcgtat gcgttatgaa gatccaaacg gtgactatgg tagacaagag cgtcaaagaa
     1561 agatcgtaga aggtgtagcg aaaaaagtat tgagtctaaa cggtgtcagc aactatcaat
     1621 ccgttttgaa tgctctagag tcgaatatga aaacagacat gagctttgat gaaatgaaaa
     1681 aaattgcttt agattatcgg gaggcatttg gtacgatcaa gcaagatcag ctgcaaggag
     1741 aaggcttcat gcaagataat atctcctatc aaaaagtcag tgatcaagaa cttgatcgtg
     1801 tgaaaaaaga attaaaagat caaatgaatc tagagaataa ataggaaagt ggagaaaaaa
     1861 tggaagagac aatcagcttg aaagaaattt ttgaaacatt acgcaaacac ttggtaacaa
     1921 tcatcatctc aatgtttgct ggcttggcca tctcaggaat cgtaacattt ttcatcttga
     1981 caccaaaata cagttcacaa gcacaattga tcgtgacact gccacaatca gacacagcaa
     2041 atgtcaatga tgtcaatacg aacctgcaaa tgatcaatac gtataaagat atgatcgtca
     2101 gtgatttagt actaaatgaa gtcaaagatc gtttagaaac agaagacaat gtgaaaatga
     2161 ctgctggaca aatcaaagat gcgatctccg tcaatcagtc acaaaattca caaatgttct
     2221 cgatccaggc aataagcacg aatgcagtga ctgctcaaca gatcgctaat acaacttcac
     2281 aagttttcca agaaaacgca aaagacgtga tgaatgtcga taaaatctcg attatttcag
     2341 gtgcagttgc caatacgaca cctgtttcgc caaataataa attgaatcta gtgattggct
     2401 tagtattagg aatgatggta ggcgtaggtt tggcattctt attcgaatta cttgatcgga
     2461 cagtgaaaga tgaaaaatat gtgacggata cattaggatt cccaatctta ggaacagttc
     2521 cagaaatgtc agcgaaagaa ttgaacgctg gtatccgtaa aaaaagaaca gctcctgcac
     2581 gaccaaaagc tacaaacgat aaaacagccc catcacgcag aagccgctca agagtctagg
     2641 aggaagaaaa atggcacgaa cacagaaaca aaaagcaaca caaactcatg cagtcagttt
     2701 gatcacatta acaaacccat cttcaccaat cgccgaacaa tatcgtacga tccggacgaa
     2761 tatccagttt gcctccgcag ctggtcagca aatcaaaacg attgtcgtga cttcatcagg
     2821 tcctggagaa gggaaatcca ccactgcagc aaacattgca gtcgtttttg ctaaatccgg
     2881 tcaacgagtt ctactagtcg atgcagattt acgtaaaccc gtcgtctata agacattcca
     2941 gttgaacaat gccagtggat taagtacagc acttagttca tcaggaagtg tagcagatgt
     3001 gatccaacga acgccagtcg agaacttgtc gattttgcct agtggaccta agccacccaa
     3061 cccatcagaa ttgttaagct cgccaagaat ggatcagatc ctagctgaag caagacagtt
     3121 atttgacgtg gtgatattcg atatgccacc agtagtagcc gtaacagatg cacagatcat
     3181 gtcttcaaaa acagatggca ctttattggt cgtacgtgaa aatatgtcac gtaaagaatc
     3241 cttgactaaa gcaaaagaac tgctagagat ggtacaagca cgagtcttag gtgtcgtata
     3301 caacggagca gaacacagca aagatgctgg ttattactac ggaaattaag gaataagaag
     3361 agaggagaca agagaaatga tcgatctaca ttgccatatc ctaccaggaa tcgatgacgg
     3421 gtcagaaaac ttagaagcaa gtattgcaat ggctgaaaaa gcgattcagc aaggcattac
     3481 gcatatttta tgcacaccac accacaataa tggaaaatac agcaatgaaa aatcacaagt
     3541 catttctcta gtcgcttcgt tacaagccga attagagaaa agacagttgc ctttgacgtt
     3601 attggaagga caagaagtac gtattaccgg aacgctcatc gaagacatcc accgagatga
     3661 aattttattt acagatttag atgatacata tcttctgatc gaatttccga cactggaagt
     3721 gcctttatat gcagaacggc ttttttttga gttgttagaa ctaggaaaga caccagtgat
     3781 cgtccatcca gaacgcaacg cgcattttcg agaagaccca aatcatttgc ttccgttcct
     3841 agatatggga tgtttggccc aacttactgc gccaagttat gttggtgtat ttggcaaaga
     3901 catccagaaa acagccaaaa caatggtcaa acataatctc gtgcagatgg ctgcttctga
     3961 tgcacacggg gtctctaagc gcacctttta cttaaaagag tgctatgagc agattgccaa
     4021 ggagtttggt aaggaaaaag tagtacagat gaaacaagtc gcaaaggatc tgatcaacgg
     4081 ggatgagatc aattatccaa tctatgaaga ggtaaaaaag aagaagtttg gtttatttta
     4141 gggggagtta aaaagtgttt atattaacaa gaagaagaaa aattgccaca ttggttgttg
     4201 tggacagctt gttattaatc gctgcaaata ttgcagcagc taaattcatg aagccgtttg
     4261 tatctatacc aatggatctt attttaatct ctatcggttt gtcgattggt ttttatttat
     4321 tttatggtag tttattcaaa gttttcactc ggatcaatcg ctatacaaat ttaagagaga
     4381 tcattgcaat ttttagttca ctatctgcaa gtgctgcctc aagtatcttc atactgctgt
     4441 ttattaatcg gcgatacagt ttacggttag ttatctttac ttatctgctt tcgctcttat
     4501 tgatcatcgg tagccgattg atctggcgta tctacatcga aaaaaaaaaa atatgagata
     4561 tgtatcagca gattctgcta aaaatacatt gattgttggt gctggcgaag gcgggcgtat
     4621 tttatacaac agctttcttg gatccaaaac ggctcaagat atccacgttg tcgggtttgt
     4681 agatgatgat ccaaataaaa gaaatacgta tctttcagga aaaaaagtat taggtgcatt
     4741 aaaagatatt ccggaattga ttgaaaaata tgacattcaa atggtaacga tcgctatccc
     4801 ttccttatca agaaaaaaat tacgacgaat cttcgagtta gtcgaatccg ctcgtgtgaa
     4861 agtcaacacg atgccttcta tagaagaact tgcttcaggg aaaattagcg tatcaaaatt
     4921 aaaaacaatt gacgtagtgg atttactggg aagagatgag gtaaagctgg atattgaatc
     4981 catcaaagat cagatcacag acaaagtcat tcttgtcacc ggcgcaggag gatcgatcgg
     5041 ttcagaaatc tgtcggcaaa tcattcaatt caatcctgca aagctattgc ttttaggaca
     5101 tggggagaac tcgatctact tgattgaccg tgagttacgc acgcatcatc aaaattgtcc
     5161 tacagaaatc gtaccgatca tcgcagatat ccaagatcgc gagaaaatca atgagatcat
     5221 ggaacaatat catccagata tcgtctacca tgcagctgct cataaacatg taccactgat
     5281 ggaatataat ccaaaagaag cagtcaaaaa caatattttc ggtacaaaaa atgtagcaga
     5341 agcagctaaa gcagcaaaag tgaaaaactt cgtcatggta tcgacagata aagctaacaa
     5401 tcctccaaat gtcatgggtt caacaaaacg aatcgctgaa atgattgtga ctggattgaa
     5461 tgaagaaggc tgtacgaaat tctcagctgt cagatttggg aatgtccttg gttctcgcgg
     5521 tagtgtgatt ccagtattca gagaacaaat tgctcaaggt ggtccaatca ctgtaacaga
     5581 ctttcgtatg actcgatact ttatgacgat tcctgaagct agtcgtttag ttattcaatc
     5641 tggcgcatta gcaaaaggtg gcgaaatttt tattctagat atgagtgaac cagtgaagat
     5701 tgtagaccta gcgaaaaaca tgatccgatt gagcgggtat agcgaagacg aaatcgaaat
     5761 cattgaaaca ggtatccgtc caggggaaaa actatatgaa gaactattgt tggataagga
     5821 aagaaatgat gaagcagttt atgagaaaat ctttgtagga aacatcaaag gctactcaat
     5881 acaaaaagtg atggattttg ttaagagcct gccgcaagac gatgaacaat tggctaaaga
     5941 cattgtcact tttgcaaatg cttcgaataa gtagggggag taaagtgaaa gcaattatta
     6001 taggagctgg tggatttggc aaagagattg cttttctatt acaaagtgtc tctagatatg
     6061 aattgattgg ttttgtcgat gattcgttaa aaatgaaaaa tcaagaatta ttgggaaaac
     6121 ctgtattagg aacaatagat tcattaatag aattagaaga agagactgct atttttctgg
     6181 gaattgcttc gccagatatc aaggagaaaa tatatcaaaa gataaataaa aataataaac
     6241 tgattttccc taatttagtt gcaccaagtg cactagtggg cataaatgtt cagttaggga
     6301 ttggcaatat tttgatgccc tatacgactt atactgctga cgtagtttta ggaaatttca
     6361 atatgatcaa tatcggctct actatcggac atgatgctca aatcggtaat tataattcaa
     6421 tttttccaag tgtaaatatt tcaggacatg ttatattagg agataaaaat gaaattggag
     6481 taggtactaa aataatacaa aatctaagaa ttggaaatat gaatattata ggtgcaggaa
     6541 gtgtagtaat aaaaaatatt aataataata ctaaaaatgt tggcgtgcct accaaagtaa
     6601 tagaaagatg ggattaagtt ggaaaataaa cgaatattat tagcatctcc tcatatgagt
     6661 gaggaaggat atgaacaaga gtatgtaaaa gaagcatttg atacaaattg ggttgctcct
     6721 ttaggtgcga acgtgaatgg ttttgagaaa gaattagcaa aatacgtggg agctagtcat
     6781 gctgccgcat tgtcttctgg aacagcagct atacatttag ctttgaaagc ggctggggtc
     6841 ggtaaagatg acatagtgat ttgtcagtcc ttgacgttct cagctactgc taatccaatt
     6901 ctctatcaag gagctatacc tgtatttgtt gatagtgatt acgaaacttg gaatatgaaa
     6961 cctgaacatt tgcgaacagc actagaaaaa tatcccaaca caaaggctgt attagtagta
     7021 catttgtacg gtttatcagc taatttagac gaaattacta aaatatgcaa agaatatgat
     7081 gttactctaa ttgaagatgc tgcagaatca ttaggaacaa cgtataaagg aaaatatact
     7141 ggtacatttg gcgactatgg aatttattct tttaatggaa ataagataat cacaacttca
     7201 ggtggcggaa tgcttgtatc taatgacaaa gaaaaggtgg aaaaagtgag attttgggct
     7261 acacaatctc gtgagaaggc acgccattat caacacaatg aaattggcta taattatcga
     7321 atgagcaata tcgttgcagg tatcggtcga ggacaattga aagttttaaa tgaaagaatt
     7381 gagaaaaaac gatatattca tagtttttat aaaaacgaat tgtctaaatt atcaaatata
     7441 caattcatgc cagaaaataa ttggaatgaa cc
//
LOCUS       DAGCWU010000133         7386 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.134, whole genome shotgun sequence.
ACCESSION   DAGCWU010000133 DAGCWU010000000
VERSION     DAGCWU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.134"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            21..476
                     /locus_tag="MBZ71_002221"
     CDS             21..476
                     /locus_tag="MBZ71_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBT4430140.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            946..1215
                     /locus_tag="MBZ71_002222"
     CDS             946..1215
                     /locus_tag="MBZ71_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430141.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            1615..1869
                     /locus_tag="MBZ71_002223"
     CDS             1615..1869
                     /locus_tag="MBZ71_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBT4430142.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(1958..3004)
                     /gene="holA"
                     /locus_tag="MBZ71_002224"
     CDS             complement(1958..3004)
                     /gene="holA"
                     /locus_tag="MBZ71_002224"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBT4430143.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(3042..5315)
                     /locus_tag="MBZ71_002225"
     CDS             complement(3042..5315)
                     /locus_tag="MBZ71_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBT4430144.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(5308..5847)
                     /locus_tag="MBZ71_002226"
     CDS             complement(5308..5847)
                     /locus_tag="MBZ71_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBT4430145.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(5983..6201)
                     /locus_tag="MBZ71_002227"
     CDS             complement(5983..6201)
                     /locus_tag="MBZ71_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBT4430146.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(6198..6443)
                     /locus_tag="MBZ71_002228"
     CDS             complement(6198..6443)
                     /locus_tag="MBZ71_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBT4430147.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(6559..7287)
                     /locus_tag="MBZ71_002229"
     CDS             complement(6559..7287)
                     /locus_tag="MBZ71_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBT4430148.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         2260 a         1466 c         1250 g         2410 t
ORIGIN      
        1 gaaagtgagg aatatatttc atggaaagtt ggcttttttt aggaattatt ttacttgtag
       61 caattttagc taaaaatcaa agtctgatga tagcggcagc ttttgttttg attatgaagc
      121 tcataccagc ctcccgttcc tttttccctt ggcttcaagg caaaggaatc aatattgggg
      181 tgacgatcat cacagcggct attctaatcc ctatcgcaac aggagaaatc ggtctacgtg
      241 atctattaga agcctttaaa tcccctattg gctgggtagc tgttttttgc ggtgggctag
      301 tggctgtctt atcttccaag ggtgtaggat tgattgcttc tgatccacaa atcactgttg
      361 cacttgtttt cggcacgatt ttaggtgttg tcttcttaag aggaatcgcc gccggaccgg
      421 tcattgcttc tggtatcact tactgtatct tacaagtatt tgctttagga aaatagaaca
      481 aattttgtct attttaggag attcttaaca cccccaattt actgatccgt gatatactga
      541 caacgaggtg atacaatgtt cgaaaaatta aagtatttca taggaacaat cggctggttt
      601 cttctagcgg tacttttgat tctgggctta gcttatctat tatttcctga tttgatacaa
      661 aatgtgtttg atactgcttt ttgattgacc tattaaaacg atatagggga tgctcctgcg
      721 atgcaggggc tttttttaat caatgttttg taataattta ggccgtgcca atgagacttc
      781 ctatagaatt cgatgctctt ttttcttttt ggctcttctg ctcttttttg ttatactaga
      841 ttacagtgga caaaagaaag aatgatcgca tccacttcaa cgaatatcaa aatggaagaa
      901 ttagtgcctg ttttcaggtt actttgaaaa gaaaggaaga cgcttgtgga aacttttgaa
      961 ggtcttttaa ttgatacgta cgttttacaa gaacagccaa tgatgctgac tgggatcaac
     1021 ttgcagcttg aggaccgtgt gatcaatggc ctgcttaaag atcgctattt ggcaaaatat
     1081 atcttacgtt tgcccctaaa taaatatgtg ctggaagtaa gtggcaaatg gaaccgtcgg
     1141 aaccagttgg tcataaaaaa aatgttgata aaaaatatcg atgaatacat taaaataatc
     1201 ggtacaccag aatagccttt actgatcaaa ggatactggt tataaaataa ctgcagtttc
     1261 ttaatagaaa aacaaagaac aacagggaat cgtatccctt ttcaaaaaaa actttgcaaa
     1321 cggttctctt tgttatataa taacttagta ccaaacattc ccattcaaac tttgtttaaa
     1381 tagataaata aaaatctagt ttggtttgac gctattgcct cccacaatag cgtctttttg
     1441 atttttttca gcttgtacta tccgttttta ctgaaaaaaa atcctaataa aagactgtta
     1501 aggaaaaaaa cttgtcatgt tcatattgca aagcacgctg cctttaggta taataatgaa
     1561 tgttgaatta tgcggtaaca cgcgtaagaa cttaagttat cggaggtgaa actaatgcca
     1621 aacattgaat ctgcaatcaa acgtgttcgt acaagcgaaa acgctaatgt gaaaaattca
     1681 tctcaaacaa gcgctatgcg tactgcaatc aaaaaatttg aagatgctgt agcttcaggt
     1741 gctgacaacg tagatgcgtt atataaagaa gctgttaaag ctattgatat ggctgaatca
     1801 aaaggtctaa tccacaaaaa caaagctaac cgcgacaaat ctcgtttaag caaaaaaatc
     1861 gctaaataaa agaatcggct tcggccgatt tttttatttg tcaaaaaaag gacaaaaaca
     1921 aacgaccttc ccgcatgctt tgccctcgtc tttcttctta tcgatttctt aacaactgcc
     1981 ctgtgcgcaa aacgaatagt tgaaaactta attctttttc gatttgtccc gttttgattt
     2041 gataatccat ctcaactaat tgatcaaaca actgtaccag caaatgttca tcgaattttt
     2101 ttacttcctg catggcaagt tttacacgat atggatggat tttcaatgtt tcagcaatat
     2161 ttgcctgctg ataaccaatc tttacaagaa attttgtctg taaaaataaa cgtaattgag
     2221 aaagcaaaat cgcattaatt ttgattgttt cttccccttg cataactaaa tcttcgtata
     2281 aacgcaaagc ttgttccgtc ttccctttta aaatatattg ggtcatgtca aaaatattat
     2341 gttctaacgt tttaggaact aattcttcta cttctcttac cgtgatttgc tgttgatttt
     2401 gtccataaag gaccaatttc tgtaactctc tcatcatttt ggataaatct aagtcggtca
     2461 acctcaagaa taaatcaatt gcacttcgtt ccattttgat tgaggaattc tctaaggtat
     2521 tcatcagata ttgacgtact tctttttcat tcaactgttg gacattgatg ataattgctg
     2581 tttttttcaa ttgtttcgtt acttttttcc gttcatccaa cttttcatat ggcgcaaaga
     2641 atactaatac agtcgattct aatggttctt tcaaataagc cgtcaataaa tcagtatttt
     2701 gttcgattcc attgtttact ttttcacctg ttaaaaaata aggattctct acgaatacta
     2761 accgttgatc tccgaaaaac ggcaacgtct ctgcttcagc tactacctca tctaaacttg
     2821 cattttccat gtcaaaactt aaaaaattta agtcatcttc gccctctaat tggatttttt
     2881 tgacaatttc tgtccgaact tgatcttgga gaaacttttc tgttcctaaa atcaagtaac
     2941 aagatgcaag tttttcttta cgtatttttt gtaactctgc ttgtatactc aaattactca
     3001 ccactttcaa aaattatcct accatgagca gatgagaatt actagtctat cattgtttcc
     3061 actagcccat gaggatgaaa aactggatgc cagcgatagg tgatcattcc ttgttgatcc
     3121 gttcgccatg tttttatctg attttcttct agtgtctcca atacttcgct gtgcgggtga
     3181 ccataacgat tatttacgcc gcaggatata atcccatgtt ttgcttcaat atgggaaaga
     3241 aaatctgtgt tacttgacgt acggctaccg tgatgaccta atttgactac atcggctttc
     3301 agattaggat aaatcaaaag caattgtttt tccattgctt gattcaaatc tcctaaaaat
     3361 aaaaagcgcc tattgttata ttccatccat agaccaatcg aatcttcatt tcttccctct
     3421 cccttttcaa aaggagtcaa tacataaaga tcataataac cgctgatcct gtccccatgt
     3481 ttgatttcaa tgatctcagt accatttttt tgagcttcag ataaatgcct ttgtaatggc
     3541 tcatttcgtc cagcgcccca accgatataa atttttttga tagtaaaatg ctgagataac
     3601 gtgggtaatt ctcccacatg atccgtatcg tcgtgtgtca agatgagttt atcgattaca
     3661 cttatcccct gaccttttag atatggaacg atatttcttt cagcaggagg acgtatcata
     3721 ccttgcgccc acttttctct ttcaaagtgt aactgacctc cagtatcgat caaaatcgtt
     3781 tcttttcgaa aaggactttg caaaaagata ctgtctcctt gaccgacatc gataaaagcg
     3841 atcgttgaag aaaagcgtaa aaatggcaaa gtcaaaaaaa taaaagaaaa aagaccaaca
     3901 taaaacaagt gtttcacagg ttgttctttc aacctatcca tacaaatcag taatccgaaa
     3961 atatacatcc cagatagcac aaggggcaaa cgtcctataa tgagagacga ccctgaagaa
     4021 aaatcgatta ttcgctcaaa taagattaat ccctcttcta tcagcaaaag taaagtttcc
     4081 ggcagccagc gattgaagag tgtgagaaat aacacaccag gaagaaaaag aaacttgaat
     4141 accggtgcaa tcagtaatgt aaaaagacta cctaaaatag gccactcata aaaatgccat
     4201 acaatcaacg gcatcgtgat catagtaaac aggacgtgag aggccgtttt tttcaaccaa
     4261 tctccatcaa aagaaatcag gataaacaaa aaactcatga aaaaactaag ctgtccaccc
     4321 gcatgaaaaa gaaatcgagg atcaaatagt aaaagaagcc aaaagacaac tgcaaataag
     4381 tccatggtgg aataatgctt attcagcaat ttgactagca atcgcaataa aaacagcaaa
     4441 ctagcacgca aaacagaaat agaacttcct gcaatagaaa cataacagat agttagtaga
     4501 ctcaacggaa taatactgac gtgtagtggc catccgatac gtcgaaataa ataatacagc
     4561 caccctaaat aaaattggat gtgtagaccc gatagactga acagatgcag caaaccactc
     4621 tccctaaaaa tttcttctgg ttctctgaat cctgcatctt tatagcccca taataaagaa
     4681 ttaatatata cacttgtttt ttgcggaaat tcagattgga caaaggagat aaaagatcct
     4741 ctttttctct gaagccagtt tcgccagtga ttttgcatgg tgatctgttc aatcttttga
     4801 atttggaacc gtccagaaat tctatggttc ctgaagtaag cttcttggtc aaaattataa
     4861 agattccgct tttttgctgg agagatgaac tcgccttcag cagtcaattc tattcccttt
     4921 cctcggaagt taagccatgt tttcaattct gcttctgtgt ttacttgata agaaagctgg
     4981 agcttttgtt tattctctga taaccttcct tccattgtca aaaaattacc atttgcacga
     5041 atcgtatctg taaatacagt aatttgcgca gatgttgttt tttcctctaa aaatgaatac
     5101 tcttgtagcc tataagaaat ggataccgtt atgctacaca aaatacacaa gtacgcgata
     5161 aaacgtcctt cttttagcga aaaccataaa aaaattccgg caagaagcaa acgataaccc
     5221 caattgtcag tgtaatacaa caaacagata atcatcgacg taattgccgg aaaaatcaac
     5281 cgatttcgaa catgcctttc taaaacgcta gacatgtttt cctgttaatt cattttgaag
     5341 ttttgaaaaa tgctgtgagt ctaaagcaac ttggtgtaca gatacaccta attgttcgat
     5401 caacgcttgg gcataaggat catttcgata gtcatggaga taattgatcc ctttcacacc
     5461 agcttgcaac aaagcctttg tacaggccaa acatggaaaa tgtgtgacat agatttctga
     5521 accttccgtc gggataccaa gttttgcaca ctgtaggagt gcattcatct ctgcatggat
     5581 cgttcgcaag cagtgtccat ccacgacgta acacccttca tctatgcagt ggacatctcc
     5641 actgactgct ccattatagc cgccagcaat gatccttttt tctcgaacga gtgtcgctcc
     5701 tacttctaaa cgtgtacacg tactacgtaa agataacagt actgcctgag ccataaagta
     5761 ctggtcccaa ggaatgcgtt cattatgtga cttatcagta actttttctt tattcgtatc
     5821 gcatgctttt gaagattttt ctaacatgtt ttatattcct ccgttttttc aagttgatta
     5881 aaaagtactc attgcttaaa caaaatattc tttaaatata acgaatcatc ctgtctgaca
     5941 aaagatccta cccttcactg ccgaaatgaa gtccgtcatc cattagccca ttataaaaat
     6001 tcttcgaatc catttgttca agaaattccg tataaaaatc gatacttcac aatcatggcg
     6061 ttttgctact tctgtgagaa cagagatata ttctttgatc aacttgttat tccgatattt
     6121 ttgttttgtc tcatcgactg ctggtggcgt gataaagaaa gcatgatcag aactatagtc
     6181 ttttttcagc tgaactatca tccactgcag attttttggt acgtttcttt agaaagttgt
     6241 ttatgctctg ctagatcatt cgtcccaatg agaataaata ctttatctgc tgtatagcta
     6301 agtacatctt tggcaaagcg ttctcgtaga gcttgactgt tattgccgct gacagccgta
     6361 ttcataatat gaatcccaga tattttattt tggagataga cattcagcat tggttttttt
     6421 aatgtttctt tgcgaacaat caagctattt cctatccaaa aatactacct cctcgtcctg
     6481 ccaagctctt agaattggat tattcttagt atccattagc gtccatttat tgctcgtgcc
     6541 agtcgatttt gaatcattct atttaattgt aacgaagttt tttagttttt caactgtctt
     6601 ttcaccgata ccagaaatct cctgcaggtc ttccactttc tggaaggatc cgttttcttc
     6661 tctaaatcga atgatttctt gcgctttgac tgatccgatc ccaggaatct gctgaagttc
     6721 tgatggagtg gctgtattga tatttatttt gggaggctcg gaatctgctg taggctgttt
     6781 tccttccgag gatgccaact gatcaagatg ttctggcagc tcttcaccta cagcagggat
     6841 atataggact tgctgatctt ctatcttcgc agaaaagttg attcgatcgc tatccgcttc
     6901 ttctaataat ccacctgcac gctttagtac atcatagacc cgttcttctg aagaaaagga
     6961 atagatacca ggctccttca cacttccttt gatatcggtg tacatgacct ttggacgagc
     7021 atcctcttct acatcgtttc cttcagctga tggatcttct ccactggaac taaacgaaga
     7081 aagtatttct tttgcagcag tctcttctgt ttctctataa aatagcaaat ttgataataa
     7141 aaaaccactt gcgatacaaa agataagaac tatcatagac atccaaatga tcaatttggg
     7201 tgaagctagt attttcttgc tgattttttc tatcattgtt cttttttcaa agaatgacca
     7261 gtaaaaatca ttcattactc ctttcattta aaattgaaga aacagtgagc tctggcaaaa
     7321 tcgtcccttc aacatctaat tacgtcaatt ttttcttttg tttttatttt tatcgtgaag
     7381 attttt
//
LOCUS       DAGCWU010000134         7197 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.135, whole genome shotgun sequence.
ACCESSION   DAGCWU010000134 DAGCWU010000000
VERSION     DAGCWU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.135"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(133..651)
                     /locus_tag="MBZ71_002230"
     CDS             complement(133..651)
                     /locus_tag="MBZ71_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBT4430149.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(653..1270)
                     /locus_tag="MBZ71_002231"
     CDS             complement(653..1270)
                     /locus_tag="MBZ71_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBT4430150.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKFGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1267..2121)
                     /locus_tag="MBZ71_002232"
     CDS             complement(1267..2121)
                     /locus_tag="MBZ71_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4430151.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2118..3029)
                     /locus_tag="MBZ71_002233"
     CDS             complement(2118..3029)
                     /locus_tag="MBZ71_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4430152.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3070..4404)
                     /locus_tag="MBZ71_002234"
     CDS             complement(3070..4404)
                     /locus_tag="MBZ71_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4430153.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4612..5442
                     /locus_tag="MBZ71_002235"
     CDS             4612..5442
                     /locus_tag="MBZ71_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBT4430154.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5686..5968)
                     /locus_tag="MBZ71_002236"
                     /pseudo
     CDS             complement(5686..5968)
                     /locus_tag="MBZ71_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6126..6320)
                     /locus_tag="MBZ71_002237"
     CDS             complement(6126..6320)
                     /locus_tag="MBZ71_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430155.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6825..7010)
                     /locus_tag="MBZ71_002238"
     CDS             complement(6825..7010)
                     /locus_tag="MBZ71_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430156.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         2383 a         1425 c         1232 g         2157 t
ORIGIN      
        1 attttataac gttctcattt aacaaaactt aagttattaa gacaaatgaa taaacaacta
       61 cgctttcctc gaaacaaaaa aataaagcac aaatgcgcat ttacgcactc atgctttatt
      121 cgtacaatta attcagataa cggcagcgca taggatgcca ataacacttc ctaaaatagc
      181 tcctcctaaa atgtcagtag gataatgtaa aagtaaaaaa atcctcgaat aagcgatcaa
      241 acaagcaaat aaaaaggcta atattcctgc aactggactt aattcgaaca acgcagctac
      301 tgataaaaag gctagcgctg tatgtcctga aggaaaagaa gaagtcttcg gtttacttgt
      361 gtaaaggaca agccgacgat ttttttcata aggtcttttc cgtcttgtcg tttttttgat
      421 aaaaagcaaa ccaacaaaaa aatagatcaa actgccgata agaatggcta gaccatattc
      481 tctagtatca ggtatcaaga ctaaaacagc agcaaggagc aaccaaataa aaccaccatt
      541 agagatatta ctgataaatt tcaaaaaatt gatttttcga ctatccgttt tccgttgctt
      601 ggcgaacttg ttgataatac tatcatcgat tttagcaatc aaacgcaaca tgttattcct
      661 cctttttaag aaccttttct tgcgtacgat ggataaaaag caggacttga aaataaaaca
      721 agaaactgaa acctaataaa gcaaaataga ttggtcctgc aaaccagaat gcgacaagaa
      781 ggatcaacgt gcctgccaaa tagaaaagcg cgcttttgaa atcattaaaa aacaagaaaa
      841 atgcagtaat ccaagaagct tctttttgtt ccagttgaag caagattttg taactaaaga
      901 gaagcgcgct taatagaagc aaaaacaacg tagtatacat caatgtatag ccaattcccc
      961 caccccaata gcggaaaatc tgataatcga tcaatagaag aagaaaataa acacttgcta
     1021 aactatcttg ctttccataa agagagaagt tctcacgaaa ctttttccag aatagtgaac
     1081 cgaacttttt ctcttcattt tttttgtaat gataaatcat atacagaact gttcgacttg
     1141 cagttcctaa tgtgaaaaca ggcaagacgc acaggaacca tagtagctgt aaatggataa
     1201 gacgtggaat ccattcaatg atttctaaga aataatcatt ggctttttta ccaatcgatc
     1261 gattcatcat ccttttgaac ctcctgtcaa atccatccct tcaatgaaat aacgttgtgc
     1321 aaaaaagaac agcaaaatcg tcgggatcaa gacaagtgtt gctcctgcca tcagataatt
     1381 ccattgtgtc gttgcttggt tctgaaaaac ttgcaagcca atctgtaacg tgtatttttc
     1441 ttgatcttgg atatataaca gaggacctaa gaaatcattc caagctgcgt tgaaggaatt
     1501 gatcgcaatg gtaatcaagg caggtttcgt caatggaagc atcaaatgac tccaaatata
     1561 caaatgattc gctccgtcga tttcagcagc ttcgatcaat tcattattga tcgataagta
     1621 aaattgacgc atcagaaata tattgaacgc attgccgaag aacgatggaa cgatcagggg
     1681 caaataagta cctacccagc cgatcttgga gaataataca tattgaggga tcatcgttac
     1741 aaaccccggg atcatcatcg tagaaagaac caaagcaaac atcagctttt tcccaggaaa
     1801 atcaagcttc gcaaatccat aagcaataaa agaatttgaa aggacattac cgatcactac
     1861 tagcacagtg atgaataatg tatttcttgc atagcgccaa aaaggaaagg cggcaatcgt
     1921 atccagatag ttttcccata cgattttttc tgggaaaaag gtcggaggat aacgaacgat
     1981 ttcttccatt ggtttcaatg aagtaaacac catccaaagc aatggaatga tcaaaatgat
     2041 tcctattact gtcatgagga cataacttag tatttttttt gtttgccgat tcacactggt
     2101 ctttttgaaa taatccatca tttgccgtca cctccagaat aatagaccca cttaggtgca
     2161 agcttcaaat tgacaaacgt cagaaccaac acgatcagaa acaggatcat tgacatagcc
     2221 atcgcataac ccatatcaaa agctacgaac gccttgttcc acatatgcag attataaaaa
     2281 agcaaagaat ttgcaggtcc gcctgttcca tttttcgtca tgacataagc ttcttgaaaa
     2341 atttggaaag aaccgatcgt agaggtgatc acatcgtaaa aaataatagg agtgattaat
     2401 ggcaaagtaa tacttttgaa ctttccccaa acgcctgctc catcgatttc tgctgattca
     2461 tataaagatc taggaacatt ttgtaatgta gctaagtaaa gtagcattgc tcctccagaa
     2521 ctccatattt tcatcaaaat caacgcaggt ttcgtccatt gtggatcaaa cagccaagct
     2581 ggtccaggga tatggaacca gcccagaaat gtgttgatca atcctgcaga tggagaaagc
     2641 agctgcatcc ataagatgta gactgctact cctgataaga ctgccggtaa atagaaagct
     2701 gtacggaata cactgattcc tttgattttt tgattgagta ctgtggctaa aaacacactg
     2761 aaaatcgtcg tcagcggtac attgaagatg acgaaataaa cggtgttgta taacgatttc
     2821 caaaataaat catcgtgaac gaacattctt ttgaaatttg ctagaccaat gaaattcatc
     2881 ttggatgtca aattataatc ggtgaaacta ccaataactg aaaaaagcat aggccctagc
     2941 ataaatacca agaacccgat aataaaaggc gagataaata aataggccca tcttgcttct
     3001 tttttctttc ggatagataa acgcttcaaa ttttcttgcc tcctctgtac ctagacagtc
     3061 aaaaatggat tattgatttt gcttgactaa gttttctact gctttttgtg catctgccaa
     3121 tccttctttt ggtgattttg ttcctaaaag aatctggtca atctgttcat tcactaatgt
     3181 cgtgtaatcc ggagcatcta aaggaactgg tgtgatcacc gtatttttaa agttttcgtc
     3241 cgccatttga tagatcattt ttcctttatc cgttaggttg tcattattca ctaaattctc
     3301 attggctgtc ttgctcgcca tgatatcaaa acttttttcg ccgaattttt cttgtacttc
     3361 tggagaagaa agatatttga tgaattcata agaggcctca ggatcttttg ctccgtatgg
     3421 tacttccaag acgaatccgc caccccacga ccaatggcct gaaccttctt ctttttctgg
     3481 gagctgcatc acgccaaaat caaaatcatc cggtgcattt tccatcaaac tagagtaata
     3541 attgatattt tgggcacgca tggctactaa tccggaaata aatggatcag atacaccaga
     3601 cccaaattcg gcttctaatt tattgatcgt atttttccca taataatctt gccagtcttg
     3661 gatccactcc aaggcttcca ctttattttt agtatcaatc ttgacttttt catctttgtc
     3721 aaaccaacta gtcccgttat ctgcgtttag cgcccataca tcagccccta aattccataa
     3781 tggataaaaa ccgatgcgtg taaattcttt tccttctttt ttatctagtt tacgtgctgt
     3841 ttcttctaat tcatcccaag ttgtcggtag atcttgttca ctgattccag cttctttaaa
     3901 taacttttta ttataaaaaa tgacttgtgt atctgtattg aatgggatcg cataaggatc
     3961 accttcatat tctactgtat cccaaagctg cggataaaaa tccttagaag tgtctttttc
     4021 tacatatttg gagagattag ttgcttgctg tgcttctgct ctttgggcaa cggaatttat
     4081 gtcctgtaca ataacatcgg gtgcatttcc agctgttatt gcagataaag atttagtcca
     4141 gatgtcaccc catggctgat acttataatt tacatggatc tcgttttgtg attgattgaa
     4201 atcctcaatg atttcttcga tagcttcttt tcgtgcaccg gatccccaaa aggaccagaa
     4261 atcaatattt tttcttccat cacttgtttt ttcagaagaa tttgctgcac ttgaactggc
     4321 atcgcttgca ttacttgatc cgcatcctcc taataaaaac aatgctgaac ttgccagcaa
     4381 ccctgtaaac aataattttt tcatatttta cctccaaatt ttaatgtaac cgatgccggt
     4441 tttaatataa cgatatcatt ttgttctgaa aataacaatt tataaggtat gattctaaaa
     4501 aaattattaa tgataccaat actttatata attcgcgagc gctttctttg aattttttaa
     4561 tctttcgtag cagaatgaga atgtattaaa aatagattag gaggaatttt gatgtttaga
     4621 caccagaaag aattgcagtt tgaagcaaaa ccagatcgtc cgaatccatt aatcgcgaaa
     4681 tatctgcagg agctgatcgg cggccaatat ggcgagatga gtgtagctat gcagtacctg
     4741 ttccaaggct ggagctgccg aggagaagaa aaatataaag atatgctgat ggacattgca
     4801 acagaagaaa tcggccacgt ggaaatgttg gcgacaatgg ttgctcgttt attagagggt
     4861 gcgcctgttg aagatcaaga agcagcgatg caaaaagatc caacaatcgg tgccattatc
     4921 tctggaatga atccacagca cgcgatcgta gctggcttag gacctcgtcc tgctgacagc
     4981 gtgggcaatc catggagcgg tgcctatatc attgcgagtg gcaacttgtt agcagacttt
     5041 agagctaatt taaatgccga atcacaagga agacttcaag tagcacgtat ttacgaaatg
     5101 atcgatgatc gtggcgtaaa agatatgctg tctgttctcc ttgcaagaga tagctatcat
     5161 caaaatctat gggaatgtgc agtgaaagaa ttagaagaaa aagaaggttc aatcttagtc
     5221 ccaagtactt tcccgcgtga caaagaacga aaagacattg cttatgactt ctataacttc
     5281 tcagagggag accaaagtag tgaaggttct tgggctagtg gcaaagcact ggatggcgaa
     5341 ggcgaatatc aatggttgaa agaacctaag atcgaaggcg ctgcaccaaa attagaacca
     5401 gtaacaccaa aaatgtatgg gacacctcca ttcaagaaat aaatatctac ccgtagaaac
     5461 acccgttttt tgttccacac gctttgtgag caaaactgac taaagtcatt aatcataacc
     5521 acccttcaaa agggtggttt tctctagagc tataagctca aatcaccggc cagcgcctaa
     5581 agacgctggc tttcatattt tatgttcaag ccatattgcc tttgccagtt ttgcccacct
     5641 cttaaaaggt ttatgtacta ctccttagcc cttaaaagga tcgtcttact ctcgtacact
     5701 cagcttatct aacgcactat catgtttttc ttgttcttga atgtactttt tgatggtcgc
     5761 ttcattttgt cctacttttg tcacatagta tccttccacc caaaagtgtc gatacccaaa
     5821 tttatatttt agatttgcac aatctcttga tgatctcctg aatattggct cgatattgat
     5881 tataaattat ttttcttcta tactttggtg tgaatacaat gtggtattta cacatctact
     5941 ttgtacgggc taaactattt ccctttattg ctccacccgc atagcaagtg gttttgtatt
     6001 tcaatcgctc ggcgcgctca attaggtcta aagacaataa atctaaaaac ccgctgcttc
     6061 actgacagaa acaacgggtt ttcagaaaaa aatgaaatga aaaagatctc ttacggcaat
     6121 ctattttatt tttttgattt tttataaatg gaaaatgctt caagaagtcc aagaaataaa
     6181 gccgtataaa aacaagaacc tatgaaatct ttatttatta gatattcaac agaaatcccg
     6241 ataacactag ccaacaatat atttatcaga agtgtaaaat aaggatgctt tttatagaat
     6301 agaactagtg ttgttttcat agactctcct ttcttcatat ttccaattaa tcacaaattc
     6361 acggcattac tagaaaaatt aaaggggcgc agaagtggaa aaatactctt cgactttccc
     6421 tacaaaccag gccatcttgt gtttaataac tataccaaat ctaaggtacc tctaaatcta
     6481 aattccattt gtggcggtga cctccaattt ttaactcttc tatctcccat ccagtataca
     6541 taaccttgat acttcgacca cttatatatc gaacacacaa atacctatcc attgcaggag
     6601 tactacctaa cttagttgct acttgcatct aaataacagc tgaaaagata ggacttactc
     6661 ttagtaaagt atctgaacta ttaattttta aactatcatt tttatcctca actacatgca
     6721 catctgttgc aacttcactt tctgtctcat ccagataaat gattaaggaa tcgggaattg
     6781 caatatcttc agctgacgct ggaatagttg agctaaatta gtcatcatat ctttattata
     6841 aaataaccaa cagcaaaaaa tacaatgaaa aacaaacttg gaactaatgt atcattaata
     6901 aatgttgaaa aattcagcaa ctcattactg tatgatttta gcagcccaac caacatatac
     6961 cctataaaag ctaataagat ggttaggaat cctttagaat ttttactcat tcatagacac
     7021 ttccttaaat aacttttatt atagaaggta ttgtaaataa taatattgac aaaaagtgac
     7081 tcaaaataaa aaaaacctta cattatactg gaatgaactg tgaatcggct acactagact
     7141 agacagaaaa aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatg
//
LOCUS       DAGCWU010000135         7127 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.136, whole genome shotgun sequence.
ACCESSION   DAGCWU010000135 DAGCWU010000000
VERSION     DAGCWU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.136"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(37..1383)
                     /gene="gor"
                     /locus_tag="MBZ71_002239"
     CDS             complement(37..1383)
                     /gene="gor"
                     /locus_tag="MBZ71_002239"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBT4430157.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1517..2770)
                     /locus_tag="MBZ71_002240"
     CDS             complement(1517..2770)
                     /locus_tag="MBZ71_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBT4430158.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(2776..3699)
                     /locus_tag="MBZ71_002241"
     CDS             complement(2776..3699)
                     /locus_tag="MBZ71_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBT4430159.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(3920..6412)
                     /locus_tag="MBZ71_002242"
     CDS             complement(3920..6412)
                     /locus_tag="MBZ71_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBT4430160.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6419..6886)
                     /locus_tag="MBZ71_002243"
     CDS             complement(6419..6886)
                     /locus_tag="MBZ71_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBT4430161.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7022..7095)
                     /locus_tag="MBZ71_002244"
     tRNA            complement(7022..7095)
                     /locus_tag="MBZ71_002244"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7059..7061),aa:Arg,seq:acg)
BASE COUNT         1948 a         1571 c         1158 g         2450 t
ORIGIN      
        1 gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta cgaattcttc
       61 cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg cacccatctt
      121 gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag catgtaagcc
      181 gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt ggcgataatc
      241 atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt cattttcgcc
      301 atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg gatgcgtaaa
      361 gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat taaataaacg
      421 ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt taccgatcac
      481 atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta ctttgataaa
      541 cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg tattcggtac
      601 acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt caaatagcac
      661 cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt aagtttttat
      721 tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa acgttctaag
      781 tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacgaa atacacctga
      841 aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta actctgttaa
      901 ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca ttttacttgc
      961 acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt ttacttcgat
     1021 cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat tgctatctaa
     1081 tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt ctactagacc
     1141 ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat ctcgttcaat
     1201 tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac cgacgttgac
     1261 acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat gcatccctgc
     1321 tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat catatatttg
     1381 cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga tcctttttct
     1441 acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt aaaacaggga
     1501 tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc accggctgtg
     1561 cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat tcgccttctt
     1621 cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca taaaattcga
     1681 tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt gttgcctcat
     1741 cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt tcaccgaata
     1801 attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct cgttgtgcta
     1861 ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga tcttccatat
     1921 aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg atcgatttca
     1981 ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga atatgttcga
     2041 tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct gtatcagtta
     2101 aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag cgacaagatt
     2161 gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat gtgcatcgac
     2221 cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta tccgtatttt
     2281 tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca cgttccaacc
     2341 cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga gtggataaat
     2401 ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga tcagaaacaa
     2461 tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct ccatcttcat
     2521 ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt tcttttgcaa
     2581 aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt cttaaaccat
     2641 atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt tttaactttt
     2701 ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt actgtcattt
     2761 ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc ctgtatctaa
     2821 ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg ctttttcttg
     2881 atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag tgatgtttac
     2941 gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat gatgctgata
     3001 aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat gtgtgccatg
     3061 actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga taaaaagacc
     3121 tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct gtagcagtgg
     3181 acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag gaatatcgag
     3241 tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta ctgctccaac
     3301 tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg tttcagcatc
     3361 ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa tacctgtatc
     3421 accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt caggtacatt
     3481 tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt taccatactt
     3541 gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag gcagacgcaa
     3601 tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca actgttctgc
     3661 ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct cctacaactt
     3721 cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa gtcggatttt
     3781 tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca aatataaaaa
     3841 gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt ataaaaattc
     3901 agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt ccgcgaacat
     3961 tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa cgaatttctc
     4021 cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg cgacggattg
     4081 gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt gcagatggtg
     4141 tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg ctcatgattt
     4201 ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc tcatcgattc
     4261 gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt ttttgcatgg
     4321 ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct tctcgaattt
     4381 gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc acttttctac
     4441 ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg aaaacgtctg
     4501 gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga cgaatttttt
     4561 ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg attaatcggc
     4621 ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca tcttcacttc
     4681 caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca gttggtccaa
     4741 ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt gcacggcgga
     4801 tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca tgcagaagtc
     4861 cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga gggacaccag
     4921 tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa taaccttgct
     4981 cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct ttttcttgtc
     5041 tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc ttttcttgta
     5101 ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg gtttgatcca
     5161 aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt agttgacgag
     5221 aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct gtgatctcca
     5281 ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct gcttcttcag
     5341 gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa tctttttcga
     5401 tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct cctcgagcta
     5461 acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct gcaccaatta
     5521 aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt tcgtcgatga
     5581 cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc aaagatccca
     5641 tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct aaaatgattt
     5701 tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc accagtacag
     5761 ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct ctcgaacgac
     5821 caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt gctaatgaat
     5881 ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca tttctacgac
     5941 gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc agaacttgac
     6001 gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg atactttcat
     6061 cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca gttttcttca
     6121 cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga agaaagactc
     6181 ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct tcataaattt
     6241 ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt tgctcgacca
     6301 ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt tttgcttctt
     6361 cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc atctaatctc
     6421 acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga gcacgtgtat
     6481 gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac atcaagtttc
     6541 cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca aaagcatttt
     6601 tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt tttttatctg
     6661 aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt tctactagat
     6721 aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa ggaacacagt
     6781 taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt tccagaattt
     6841 tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta ttcattccct
     6901 ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga agaacgcttt
     6961 ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta ttccgtatcg
     7021 acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca ctctatccag
     7081 ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttg
//
LOCUS       DAGCWU010000136         7091 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.137, whole genome shotgun sequence.
ACCESSION   DAGCWU010000136 DAGCWU010000000
VERSION     DAGCWU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.137"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(433..846)
                     /locus_tag="MBZ71_002245"
     CDS             complement(433..846)
                     /locus_tag="MBZ71_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBT4430162.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1302..1787)
                     /locus_tag="MBZ71_002246"
     CDS             complement(1302..1787)
                     /locus_tag="MBZ71_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430163.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1926..2141)
                     /locus_tag="MBZ71_002247"
     CDS             complement(1926..2141)
                     /locus_tag="MBZ71_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBT4430164.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2365..3165
                     /locus_tag="MBZ71_002248"
     CDS             2365..3165
                     /locus_tag="MBZ71_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4430165.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3184..5052
                     /locus_tag="MBZ71_002249"
     CDS             3184..5052
                     /locus_tag="MBZ71_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBT4430166.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5045..5536
                     /locus_tag="MBZ71_002250"
     CDS             5045..5536
                     /locus_tag="MBZ71_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBT4430167.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5524..6078
                     /gene="srlA"
                     /locus_tag="MBZ71_002251"
     CDS             5524..6078
                     /gene="srlA"
                     /locus_tag="MBZ71_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBT4430168.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6096..7091
                     /locus_tag="MBZ71_002252"
     CDS             6096..7091
                     /locus_tag="MBZ71_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBT4430169.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2494 a         1132 c         1370 g         2095 t
ORIGIN      
        1 aaataaaaaa cttccagcca aaactgactg gaagtttatt tttttctgaa atattctaac
       61 acaaatatga aaaagatggc cgaagctaat aagccgattt cgtagcaaaa caatgagata
      121 ccagagtcgt aataataaaa tcctctaatc ttatggataa aatagatcat cggacaagca
      181 gataagagta agtatttgtt aggccttttg aataagtaaa agaaagcact ggtcattatg
      241 ataaatggta tccaaaacaa aagtttactt aagacaatca aatgatcaac tcccatattt
      301 tttaccaaga gagatcataa catctttatc gtaaaaacaa aatatttttt tgctcatttt
      361 gaaaatttaa acatactcgt tcttatccaa atatataaaa atcgcccctc attcatcgag
      421 aggcgataag ttctaattta actcaatttt ttttgtttct tttggctgat aagctgtttt
      481 aggtaatacc aatttcagca caccgttttt aaaggttcca gtgatttttt cttcatcaac
      541 attaggaaga tagaatgaac gtttgtaaga gaccgaatgt ctttctttcc gtacatagtt
      601 gccatcttct ttatcttctg tatgggactc atgattggcg gagatcgtca aagtattatc
      661 tgaatattca actgtcgtgt cttctttgtc acagccaggc atatcagcag ttagtttgta
      721 ttctttttca ttttcgacta aatctacttt tggataactt gaaacaccta aaaaatcatt
      781 gaatgctgga ctaaacacgt cattgaaatc agggaacatg tcgcgaatgc taggtacatt
      841 tgccattttt gatcatcctt tcaataagta ttttttattt atccctattt acgtaataac
      901 tctattttga aaaaaatgca agtataatgg ttagctttgt gaatatttca tctaatacga
      961 tacaaaggct atctttctaa tttgaaaaag ggatcattaa aataataaga agaaaatatg
     1021 tcaaaaggta aaaaagtaaa ttcttctatc caaatataga aaaattgaat ctagaaaacc
     1081 gcccgacaat aaatttcatt aactcagttg accgaaaatg aagtagatac caaaaattta
     1141 tcaagagagc aagtagagaa gtgagtatat tcataagaga aatagctgta acgaattatt
     1201 aggttaatgc aaatgcttat ttatactccg tataactttc tcagaaaaaa tgataccaat
     1261 aatgacttgc tagtactgta aatccgtata atatcatcta atcaaaatgg tgttccattt
     1321 gcatctgtca tcgtaatcga tccttcgggt gacactcgat aaaaaccaac gcttccagat
     1381 cctccttggg aacggatttg tttgctggaa gctgtgaaca aataatctcg atcaattttt
     1441 tgataaaagc taataacaat atcgtcactt tgtaattcgg aacggtgggc aataagaaat
     1501 agaagtgctt tgtcagagtt ttctaatgga agagaaggta cagtgctttc ttgcatttca
     1561 gatgttgaag aaatgctaaa cgactgaata cttgattggc tatgttgcga atctacatta
     1621 ccagtagaag tattagtaat ggaagtagta gaagtacttt tagttgaacc aacctcagtt
     1681 gttgaaatgg ctgctgaaga ttctttaaca gaattctttg ttttatatcc acaggcagaa
     1741 aaaagtatta cagttcccaa caagcctgcg taaatccctt tatgcatgat agctcctcct
     1801 aaaatagatt atctttattc agtatatcac ttaaacaaag aaaagcagat ggtttaaaat
     1861 gaaaaaacct tcccaataaa gggaaggttc atgaagataa taactttttt aataattata
     1921 gctttctaat atctttcatc aacttttcta gtaatcattg ggaaaaattt caagcagtag
     1981 atcgcacaaa tacctacgac gatataaatg atcttcgcaa aaatagttgt tgaaccacca
     2041 gcaatcatag ctactaaatc aagttcgaac aagcctacca acagccagtt caaccctccc
     2101 acaatcaata aagttaatgc aatagcatct aaagttttca ttagaattct cctttcttaa
     2161 atgataaaac aattatatat caaaaagaga aacattgctt atataacgct caggaatttt
     2221 agcaatctat gcaaaaagat gaaaattttt tttgctcttc cattagtaca cattttttag
     2281 ctgaagctgt aaatttcatg tcttaatttt ttttagtgag cttttatact ggatttgtaa
     2341 gaaaaaagaa ggagaggatc acatatgtcg gattggttgg atattgcagg aaaagtagta
     2401 attgtaactg gaggttcctc aggaattgga agaagtatcg tcgaaaattt gttgaagcag
     2461 aatgcacaag tagcgaattt tgatgtaact gaatgccgta tacagcatga aaacctacta
     2521 tctttaaaag tcgatgtgtc ttctaaaacg gacatagaag aagggatata taaagtaatg
     2581 aaacatttca agacgatcga tggtctagtc aataatgcag gaataaatat tccttctctt
     2641 ctaatagata gaaatcatcc aaaaagcaag tatgaattat cagaacaagt ttttgataag
     2701 atgatcgctg tcaatcaaaa aagtgtatat ttgatgagcc aagcagttgg aagaatcttg
     2761 gtgcaaaagg gatcaggagt gatcgtgaat ctttcttctg aatcaggatt ggaaggatca
     2821 gaaggacaga gctgttatgc tgctacaaaa gcagcgatga acagttttac tcgttcttgg
     2881 gcaaaagagt taggaaagag gaatgttcgt gtggtaggtg ttgcaccagg gatactggaa
     2941 gaaaccggtt tgagaacaca agaatatgaa gaagctcttt cttatacgag aggtataagc
     3001 gtcgaacagc tgagaaacgg ttacagtcga acaagtacga tccctctagg aaggagtggt
     3061 aagctgcaag aagtggcgga tctagtctgc tattatcttt cagaaagatc aagttatatc
     3121 actggagtca cgactaatat ttcaggaggt aaaacaagag gataaaggat aggggggacg
     3181 cacgtgacgc tagtaaacag atggtaccaa ttattgaaat tactagtcac tcacaagaaa
     3241 gtttcaatcc aggaagtgca aaaagaaatc aaaaccagta atcaaaccat aaaaaaagat
     3301 attggggagt tgaatgaaga aattgccggt attgcaaaaa tcatccaaaa aaacaaacat
     3361 tttgaacttg aaatttatga ttttgatttt tttgatgaga tcatgcaagg aaaattgaaa
     3421 acagccagtg atttcaattc ggcatccaaa agaatcgctt atttactaaa taggctgatc
     3481 gaaacagagg atttcttacg tatggatgat ttgtctgaag aagtcggtgt cagcagagga
     3541 acagtggtca aggatctaaa gacattaaaa gagatgatca atgattttga tgtaaagatt
     3601 acaggcacac caaataaagg actaagaata ataggagatg agctagaatt acgactactt
     3661 ttattttatt ttgtttctcc ctacttttca gagacgaaaa cagaacagtt tctagaagaa
     3721 aaacaagcaa tcaaacattt tcagaagaag acgaatgcta gtaaacaaga aatcaccctt
     3781 ttaacgaaag ttatcggtat ttctttgaat agaatctcta gtaactatct gttgaagaag
     3841 cccatcaatc gttacagtgg gtgttttgaa tatatccaag aattcaatga actgatcaat
     3901 cagttggaag aaatctatga actaactttg agccaatatg aaaaagattt tatcagtttt
     3961 cctttgaaca tcatcaacac aagtataacg atgataagga aagctaactc tgatttgcgc
     4021 ttatatttcg accccatgat gatgcgtatt cgacgattag atctcattga tctggatgag
     4081 tccttcttat atcaagagat gaaacagcac ctctctttat tgataaatcg ggtaattttt
     4141 cgttatcgat tgactgattt attttatggt gagatagaaa aaaagtatcc tttttcttac
     4201 caaatcgcta gcgaatgcat acaagcatta gaagctatct tgcaacataa gatctctaaa
     4261 gtagagatca gctacctaac gttgtatttg gagttagcat ttcgatatca aaaagagcat
     4321 ttttcctcta aaaaagtagc cattgttcat acgacaggaa aaggaacagc attaatgatc
     4381 caaagacaaa tcaaaaggat attggggagg gatatacaaa taacacatgt ttcagaagaa
     4441 caatatgaaa aagtagatct aaacaaatat ttcgctgttt ttacaacgat tccgttaaaa
     4501 aatgtaaatg aagagacacc ggtgatccat ttgactaatc tttttgatga ctcttggtta
     4561 agggcacaat ggcaaaaagc aaaaaaaaag cgattagccg ggtttcattc tactgttttt
     4621 ttctatcaaa tatggaaaga aaaaaagacg tatcaagaat atttaaacaa attagtcgct
     4681 cctttgatca gaggctatta tgtggatgaa gaatttgaaa aaaggatctt gaaaagagaa
     4741 tatcagcaaa gcacatttgt taacaaagaa tttgcttttc ctcacacaac caaccagcta
     4801 tctcaacaca ttgttttatc agtagggcta ataactgatc aaggtttagc agaaacagga
     4861 ggaaatggag ttattttttt atttgccata cctgaagaaa tgacggctga agttgagaat
     4921 gaattatttg agctatacga tatcctattt gaactcataa aaatgggaat agatgagttt
     4981 attgaattag caccgacaca agaatcattt ttaaaactaa tcaaaagtaa aggagagaga
     5041 cttcatgaat gatatgtatc tattcatagt tttcgcagct tcagcttatg tgctgcaagc
     5101 agttatcagt tggaagcaac taaagcattt caatcaaaca tatagcgaac tgcgaagaaa
     5161 aggaaaagta gcgattggta aacgacctgg gaaaatccgt tcaggcacga ttgttatgct
     5221 agctatagac gatcatgatc aagttttaga agtcagaaaa atgcaagggg ttacatcttt
     5281 ggcaaaattt cattcattac ctgtctatgc gggtgaagat atccattaca tcgatcgtta
     5341 ccatccctca gttcgagcgg aagatcgttt gacacaagca gctttagaaa atgccagaga
     5401 agtatactta cgaatagagg caggaaatga aaaaagtgaa caaccttcat tgccttttat
     5461 tgctatgctc aataattttc ggatatggaa aagtacgatc aaagaaaaaa ggagtgacaa
     5521 acgatggata tcataacaaa atttgctgag ggatttatga ggctgttcca aacaggtgca
     5581 gaaacattca taagctggat gacaggaatc gtaccagttg tcttgatgct gatggttgca
     5641 atgaatacgc tgattgcttt tttaggtgaa gaccgagtaa cgaaagtagc caaattatca
     5701 gcgaaaaatc cgcttactcg ttaccttgtg ttaccgttct tatctgcttt tatgttggga
     5761 aatcctatgt catttacaat ggctcgtttc cttccagaat actacaaacc aagctattat
     5821 gctgctcaag ctcaattttg tcatacgagc aatggtgtat tcccccatat taatcctgga
     5881 gaattgtttg tatggatggg gattgctcaa gggatcgaaa ccttagggct aaattcgatg
     5941 gagctggcta tccgatactt gcttgtggga ctgctgatga actttattgg tggttggata
     6001 actgatttta ctactggatt tgtatgcaga cagcaaggaa tcgttcttag caaaaaagta
     6061 gagctttcag ttgattagag aaaggagagt tttaaatgta tggtgatatt aagatcgtaa
     6121 gaggaaacgg cgggtatgga ggtcctttga ttattaggcc gacaaaagat aaacacaagt
     6181 tcatctatgt cacaggtgga ggtgagaggc caatgatcgt agataaaata gaagaactga
     6241 caggtatgga gtctatcaat gggttcaaaa catcaatacc tgatagtgaa atcgcgcttg
     6301 caatcgttga ctgtggaggg actcttagat gtggaattta tcctaaaaaa gggataccaa
     6361 ccatcaatat cgtttccaca ggaaaaagtg gcccattagc acaatatatc actgaagata
     6421 tttatgtttc agcagtaggg ttagaacaaa tacagccact aatggaaact gatgataaca
     6481 aagaaacagt aaatactgca tctccagata aaaagtacaa atatgatact tctaaaaaga
     6541 taaccgagca aaaagcagag cagcaaaact ggatagcaaa aattggaatg ggcgcgggaa
     6601 aagtagttgc tacattcaat caatcagcaa gagaagcagt agatacgatg atccggacag
     6661 tgatcccttt tatggctttc gtttcccttt tgattggtgt gatacaaggt tctgggatcg
     6721 gtaatgtatt tgctaacatt atggcacctt tagcaggaaa tgcagttgga ttaacaatca
     6781 ttggctttat ttgttcactg ccatttttat ctcctttgct tggtccaggg gcagttatta
     6841 gccaagtgat tggaacattg atcggtgtag agattgggaa aggaaatatt cctccccaat
     6901 tagcattacc ggcactattt gctatcaata ctcaaaatgc atgtgatttt attccagtcg
     6961 gtctaggttt ggaagaagca aaagctgaga cagtagaagt aggtgtacca tcagttcttt
     7021 attcaagatt tcttaacggc atcccacgag ttatcattgc ttgggtagca agttttggat
     7081 tatatagctg a
//
LOCUS       DAGCWU010000137         7040 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.138, whole genome shotgun sequence.
ACCESSION   DAGCWU010000137 DAGCWU010000000
VERSION     DAGCWU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.138"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(576..1592)
                     /gene="tsaD"
                     /locus_tag="MBZ71_002253"
     CDS             complement(576..1592)
                     /gene="tsaD"
                     /locus_tag="MBZ71_002253"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBT4430170.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1589..2053)
                     /gene="rimI"
                     /locus_tag="MBZ71_002254"
     CDS             complement(1589..2053)
                     /gene="rimI"
                     /locus_tag="MBZ71_002254"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBT4430171.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2060..2602)
                     /gene="rimI"
                     /locus_tag="MBZ71_002255"
     CDS             complement(2060..2602)
                     /gene="rimI"
                     /locus_tag="MBZ71_002255"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBT4430172.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2586..3308)
                     /gene="tsaB"
                     /locus_tag="MBZ71_002256"
     CDS             complement(2586..3308)
                     /gene="tsaB"
                     /locus_tag="MBZ71_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBT4430173.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3499..4479)
                     /locus_tag="MBZ71_002257"
     CDS             complement(3499..4479)
                     /locus_tag="MBZ71_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4430174.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4503..5987)
                     /gene="galT"
                     /locus_tag="MBZ71_002258"
     CDS             complement(4503..5987)
                     /gene="galT"
                     /locus_tag="MBZ71_002258"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBT4430175.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(5999..6988)
                     /gene="galE"
                     /locus_tag="MBZ71_002259"
     CDS             complement(5999..6988)
                     /gene="galE"
                     /locus_tag="MBZ71_002259"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBT4430176.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1904 a         1547 c         1252 g         2337 t
ORIGIN      
        1 atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca gttttagatc
       61 actggacaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt cactgtttgc
      121 aaaaaatact gttagattaa ctagatgaag cgtcttgtcc tcatccgatc tagattattc
      181 gatattcctc gacaataaac gattactcag tgagggcaat cagaggaacg gataatgata
      241 aataagctgt aaaaaatagc aaaataacaa taatgattaa aagtatgtaa aaaatagggc
      301 tttgacaaaa acgtgtcaca gccctatttc gattaaacgg tgtgtacctg tcaactcttc
      361 ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg ttccttgtga
      421 atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa agcttgcata
      481 cgcggtaata agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt
      541 attacttgat gcagggcagc tttttcacaa cctctttatt cagataaaac caagcttgga
      601 tcagcattca actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct
      661 ccaatcattg cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca
      721 gggagtttag ttgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca
      781 cctccagcta taatcagttg cttgactgga taattttgac aagctcgaag tgttttagta
      841 atcaatacat cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct
      901 tctccacgct gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa
      961 ctgaaatcat aattatcttc atggatcatc gctcgcggaa aatggtaatt atcttttccc
     1021 tgatgagcta gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca
     1081 actttgtcat aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat
     1141 ccatcttctt gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat
     1201 ggaaactgga atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca
     1261 ggaataagcg gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc
     1321 aacgagccga caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac
     1381 acaccagctt ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg
     1441 ctagctactt caggtactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg
     1501 atgttagata agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt
     1561 gattcaatcg ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc
     1621 tcataatgat cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac
     1681 gaaacttttc tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa
     1741 aaacatgcag cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat
     1801 gcccttttcc ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta
     1861 ctttcgagta tccaataaag ccacagatct ggttatcttc agctaaaagc agataatcag
     1921 agtgaggctg ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat
     1981 aagcattttc gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt
     2041 ttctgcaaat caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt
     2101 cttttgtttc agtgtaatat ctttttttga tcgttgttgc ttgaaacccc aatttccgat
     2161 aaagtctttg tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat
     2221 tcattatagc aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat
     2281 atgccggtcg aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga
     2341 taaaaccaat cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact
     2401 tcaattctga tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt
     2461 cgacatccag cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat
     2521 cgatttgttc gactgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat
     2581 acgctctaaa ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt
     2641 tcttctgctt ctactcgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt
     2701 tcaactaaag cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact
     2761 gtattaatca tcgcactagg caatatttcc tttatttcag agaagaattt gtgacaatca
     2821 ctcccgacga aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca
     2881 gaaatatgta catcttcaag tacagtttct aatctatttt ctttccagcg gtatgcccca
     2941 gcgtatacat tttttcttcg agcatcaaac atgggcacaa taatattatc cacaccgatg
     3001 caattagccg cgatggtttt cagactagat actcctacta gctctttatc taatgtataa
     3061 gcaatcgttt ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca
     3121 gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg
     3181 attgcaggca tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga
     3241 atttgttcgt cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat
     3301 gtaatcatat atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt
     3361 tttattctga ggatgaaaat accaaaggca aaactagtac aataatacat gattatctgc
     3421 tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc tgcggtatca ctccattttc
     3481 ccacagcttt tccatctttt atttcgttga ttctcgaata atcaactctg tttccacaat
     3541 aatctttctt gaaaccggtg ctttggaatt acttaattga agaatcgtct ccactgctag
     3601 ctctcccatc cattcggtat aaacttttac agtagaaagc gagggcgtta catatttcgc
     3661 tacactgaca tcattgaagc ccatcacaga aatatcttct ggtaccacta aacctttttc
     3721 ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg
     3781 aacatgcgga ttcgttttta aatactgctt cactgcttga tatcctgact cgacagaaaa
     3841 atctgcttcg ataattggac cttcgtcgta gcctttttct gccaaaactt gttgaaatgc
     3901 cataattctg ggatctttta gccgctgatg gtttttcttc gtatattctg ttccagcaag
     3961 catagcgata tgttgcgctt tatgggagac aaagtgttca atgacttttt gaacacttga
     4021 cttaaagtcg acaactagag agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag
     4081 caaggcattt tgggatcctt tcatttctaa taactgagct tcgtcgaatt ttcctagtgc
     4141 gatcgttcca tctgccggaa gatcactaat ggaatcccac gtttctttca ataaagtaac
     4201 gtttgcttct tctgcctttt tttcgattcc taggcgaata gataaataat aaagatcttc
     4261 aagctcttcc gtatcgtcgt accactggat caggcgaagc gtttgcttag tatttgctgc
     4321 atttttacga tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt tttgctttgt
     4381 ggctaagcca acggaaagat caggatcata gttgagaact cttgaaacag tagcaggaga
     4441 aactcctgcc aactgcgcaa tgtctttaat tgttgccatt tgtctctcct ccttttttac
     4501 gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat
     4561 actccagcgt cttccagtac acgtgcaaaa acctgaccta cagatttttg tacgacatcg
     4621 gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta attgttctgc ccacggacga
     4681 tgatattcag ccatctggtt ttcttctcct aataagaaat gctctacttc tactagttct
     4741 ggtttcaaac gaggtggtaa aacagcaagc cccattactt cgatcaaccc aatgttctcc
     4801 tttttgatat gttggacatc tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa
     4861 acattgttgt ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt
     4921 ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag ctctgataga tactgtttca
     4981 tccgaatatg ctttccattt ttccaatact gagttagctg cttcaataag caagtttttc
     5041 tctgttgatt gcaaacggat cacagacatc ggccatttca cgactcctgc gatgacttgc
     5101 ggataatctt ctagtttgat aagtttttct atcggtgctt ttgccatagg aaattcatga
     5161 cgtcctgcct gataatgatc atgagaaaga atcgatcctc caacaattgg taagtcagca
     5221 ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc gaagcagtct agcaaatgtt
     5281 tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa
     5341 taagcataag gggaatattg gaatccccag ctttcaccgt ctagattcat tcgaataatc
     5401 cggtgattgg tgcgggctgg atagttcaag cgacctctat atccttcgtt ttccatgcaa
     5461 agcatgcagg taggatagtc tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga
     5521 tctttttctg gcttagataa attgattgtg atttccagtt caccatactc cgactctgcc
     5581 ggaaatttga tgttttttgc gatggctctt gttttgatgt agtcattttt cttacttaaa
     5641 ttaaagaaat actcagttgc ttcctctggg cttttcgaat agtgctgtgc aaagaatgca
     5701 ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt
     5761 tctgctggca attctgaaag aacaccattt tctcttgcct tagcgacaag ctgatccaat
     5821 aagtctacag attcttcctt aactggtctt acttcaatgc tttctagagc atcttctcct
     5881 accatcccta aaagacgatt ctgtaaatat aagcggtcca tttccatcca tccgccagct
     5941 tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga ttgacatatc tcattcccct
     6001 attcttcata accatttgga tggctgacat gccaatccca tgctgttttg ataatcgctt
     6061 tgatatctgt gtattcaggt ttccagccca gaatctctct cgctttttca cttgaagcga
     6121 ctaatcggct aggatctcca gctcttcttg gtgctatttc tgctggaatt tcttttcctg
     6181 tcacttctct agctgcttct agcatttcct tgactgagta tcctttgctg ctgcctaaat
     6241 tgaagaagtt gctttcattt ccttctttca agtattccaa agctaaaata tgtgcagcaa
     6301 tcaaatcttc tacttgaaca taatctctga tacatgttcc atctggtgta tcataatcgt
     6361 ctccataaac agccaaggct tttctttgac caagagctac ttgtaaaatg attgggacaa
     6421 gatgcgtttc tggtgtatga tcttctccga tagatgcatc tgcttttgct ccggctacat
     6481 tgaagtagcg caaagcgaca tagcgcatgc catatgcttg gtcacaccac ttcatcattt
     6541 tttccatcat caatttactt tcaccatacg gatttttagg attagctggt gtatcttctg
     6601 tgattggaga ctctttcggc tctccataag ttgctgcagt agaagagaat acgattttat
     6661 tcacgttgaa ctcgtgcatg acttcaagta agatttgcat accgtatacg ttgttgttga
     6721 aatacatcaa tggtttttcg acagattctc ctactaaaga gcttgcagca aaatggatta
     6781 ctccttcaat cggttccttt tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc
     6841 cttcataaaa atgagcatct ggatgtactg cttgtttgtg acctgtgagc aaattatcta
     6901 ccacagctac ttgatacccc ttttgaacta gctggtctac agcgtgcgaa ccgatataac
     6961 cggctccgcc tagtactaaa attgtcataa aaaacactcc tttgattagt aaaattgtgt
     7021 ttgttgattc tatataattt
//
LOCUS       DAGCWU010000138         6909 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.139, whole genome shotgun sequence.
ACCESSION   DAGCWU010000138 DAGCWU010000000
VERSION     DAGCWU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.139"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1397)
                     /locus_tag="MBZ71_002260"
     CDS             complement(<1..1397)
                     /locus_tag="MBZ71_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBT4430177.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKM"
     gene            complement(1661..2638)
                     /gene="guaC"
                     /locus_tag="MBZ71_002261"
     CDS             complement(1661..2638)
                     /gene="guaC"
                     /locus_tag="MBZ71_002261"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBT4430178.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            complement(2895..4100)
                     /locus_tag="MBZ71_002262"
     CDS             complement(2895..4100)
                     /locus_tag="MBZ71_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4430179.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            complement(4102..5628)
                     /locus_tag="MBZ71_002263"
     CDS             complement(4102..5628)
                     /locus_tag="MBZ71_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBT4430180.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            complement(5682..6770)
                     /locus_tag="MBZ71_002264"
     CDS             complement(5682..6770)
                     /locus_tag="MBZ71_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBT4430181.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
BASE COUNT         1909 a         1599 c         1114 g         2287 t
ORIGIN      
        1 ttcatctttg aataaaacaa cggtatttgg ttttcatcca gcctactgta attgattctg
       61 attccattta tatcttgccc aaaaagtaga cttggtgcaa ttccaagacc catatctact
      121 actttttctg ctagttcggg gtctagtttt ttcccatccc aagtaatcca ggcatagtaa
      181 ccacctaaaa ttgaatgaag cttgaaaaac gattgtgaat ctccccaagc gcttatcagt
      241 cggctttttt ctttcaactt ttttcttaca tcttgcattt tcaaatcaat tgcttcatta
      301 aatacattag ccactgtgat ttgtgtaagg atattcatcg aaaactccat catttcttgc
      361 gcttgagcta gttgcgagat aaaagtctca ggtcctatca cccaaccgat tttcgttgtt
      421 tttcctaaaa ccctagataa cgaaccgacg tataacacat tatccggatc aatcgttcgt
      481 ataggaagga cttcttcttg gtttagaaat gaaagatccg taaatacatc gtcttcaata
      541 attggaagcc gatactgtcg acacaattct actagctttt ctcgtcgttt ttgactcatc
      601 acttttcctg tagggttttg aaaattcggg ttgataagca ctgctttcaa tttattttta
      661 tggatcgatt ctttcagttt ttctaagtcg atcccttctt catccatcgg tatcccaaat
      721 aaacgcgttc ctgaagcttt gaaaagaggt aatcggtaaa aaaaagaagg tgactctaca
      781 gctatcccct ctccagctga aagaatcgtt tgtaaaatca aaaagattgc ttgctgaccg
      841 ccaccagtca caagaagctg gttgggggaa aatgccaatt caaatttact ttccaaatac
      901 cgtttgattt tgttacgtaa cggagtatag cctaaaagat ccatttcttt ttcttccgct
      961 aagatttctt caagagaata agaaggaaac tcaaaattcg gcaaaagctc atctggcatc
     1021 tcgctagcgt ataaatcaag aaacgatgtt ttttggatct gctttgcttc tcttattttt
     1081 tgctcatacc agtccgtctg ctcataacga ggagagatca tttgccgcca atcgattctt
     1141 ggaacagcga atttccccca ttcagtctga ttaacaaacc atccgctgcc acgtttgctc
     1201 gttagtaccc caagctccct aagctcttcc aatgcatgga caacggtgga acgattcaca
     1261 ccaaaaagtt gccctagttt tcgttctgat tcaagttgac ttccaggatt gatccttccg
     1321 ttttcgattt ctctcataat ctgttccata atttgacgat aaatcggtcc ttgttttttc
     1381 tctacttttt cccacataag cttctctttt ctattgtcta ttttttcttt tgattttacc
     1441 gaatttacga gaaaaaagaa aaactttttt tgcaaaacat aagggactgt gacaaaaatc
     1501 ttatcacagt cccttattct gaataatgat gttcaaaagc ttgtatctgc gataataatt
     1561 ccagccaaac aaaacatata aagaactatt tttgtttggc tgttcttatt acttaatgca
     1621 ggacaacttt ttcacaacat cctaatattt tatttatttt ttagattgtg tctccattaa
     1681 aaatcgaatt tttcacgatg acatagtcaa cttttcgaat ggcttccaga tctcttcctc
     1741 cagcataaga aatagatgat tgaaggtctt gttgcatttc cactagtgta tctttcaaag
     1801 atcctttgtg ttggatccag attttctttc cttcaacatt tcgtttttcg cctttttgga
     1861 attctgaagc agaaccaaag tattctttat acacgattcc attttcgact tttgtttcgc
     1921 caggagattc ctcgtgaccg gcaaacaatg aaccaatcat gaccattgtc gcaccaaaac
     1981 ggacagattt tgcaatatcg ccatgtgttc tgatcccacc gtcagcgatg atcggcttgc
     2041 gagctgcttt cgcacaccat ctaagagctg ccagttgcca gcctccagtc ccaaagcctg
     2101 tcttgatttt tgtaatacat acttttcctg gaccaatccc gacttttgtt gcatctgcgc
     2161 ccgcattttc caattcacgg acggcttccg gtgtaccaac attaccggca atcacaaaag
     2221 tttcaggcaa atatttttta atatgctgaa tcatatcaat aactgagtta gcatgtccat
     2281 gcgcgatgtc gatagtgata taatcaggat ttagcgattc ttctgccaac ttctctataa
     2341 aggaatattc ctctttcttg acgccaacac tgatcgaggt aatcaatcca cgctttttca
     2401 tttttttgat aaacggaatt cgagctgctt catcaaaccg atgcatgata tagaaataac
     2461 cattttccgc taagaattct gcgattgttt catcaataat cgtctgcatg ttggcaggca
     2521 ctactggcat tttgaatgta tgtttaccta atgtgaccgt tgtatcacat tcagaacggc
     2581 tatttacgat acatttatta ggaattaatt gaatatcttc gtaatcaaat actttcatta
     2641 cattatcctc ctgatgttca catccagcaa aacacgaaca ttattaattt aatcaaggct
     2701 ttttttagcg ccctatgtaa tttaactcaa ttaagcaaaa aaatcaagag caaaaagaga
     2761 atttatacga atggtaaaaa aacatatatt atttttagtc tatacaaacg aaacaatgaa
     2821 tgttcgcttt tagcgaaaaa aagactgtga caaatgtctt gtcacagtct tttttctgaa
     2881 taaccggcgt tctcctacgt tgttcgtttt ttcttattat acaagtctag cacaacggct
     2941 aaaagcaaga tcaatccttt gattgcctgc tgccagtcta ccccgacgcc taaaatcgac
     3001 atgccattgt tcaatacacc catgaccaag ccaccgacaa tcgcacccat gattgttcca
     3061 atgccgccgg aagtcgaagc accaccaaaa tatactgccg ccattgcatc cagttccatc
     3121 gatgtacctg cttgaggtgt tgccgcattc aatcgagcag ctaaaactaa tccagctaaa
     3181 gctgccatga ctcccatgtt gacgaatacc cagaacgtga ttcgctttgt tttgatccct
     3241 gatagttcag ccgcttttcg atttccgccc gtcgcataaa tctgtcgacc tgctactgtt
     3301 ttatttgtca aaaacccata tgctgcaacg atcaaaccaa gaatgatcaa aataatcgga
     3361 tacccttgat aagaggcaaa gatataagtt attcctagta caacgactgc tgtaaatacc
     3421 gcctttacca agaaagctgt tggagattcc acttcaaata aatgattttt ccgacgattt
     3481 cttgttcgcc attgaccaac aactaacacg atcgtgatga tcattcctaa aatcaatgtc
     3541 aagaagtgaa ggctgcttcc ttcgcttaag tctggtaaat aacccgtaga cattttttgg
     3601 aaaacagctg gaaaaggtgc tagagattgg cctcctagaa caacttgtgt cagtccacga
     3661 aacatcagca atcctgctaa agtcacgata aacgccggta tccctgcata agctacccaa
     3721 aaaccttgcc atgcgccgat cagcccgcct actgcaagac atgcgataac tgtcatccac
     3781 gggttcattt ggcgattgac catcatcatg cccgcaattg caccaacaaa cgccatcact
     3841 gaccctaccg acaaatccac atgacctaag agcacaacta ataacatccc tgcagctagt
     3901 actaatatat ggctattttg cagaacaagg ttagttatat tcaatggacg ccagaaaata
     3961 ccatccgtca tcaattgaaa cgcaataagt agtcccgcta aaatgatcac catgctgtag
     4021 cgaccaaaaa gatcagctgc tctacttttc aaatttagtt ttttctcttt tttatccgta
     4081 agattttttg cctgttccaa cttaacccac ctcttctttc gtcattaatt tcatcaagac
     4141 ttcctgatct gcatcttctc gcgagacttc tcctgtaata cgtccttcgt tcatcgtata
     4201 gattcgatca cacatcccta atatttcagg aagttcagaa gagataagac agacagcttt
     4261 gccatttgct gccatttctt cgataatcgt gtagatctca tattttgccc caacatctat
     4321 cccacgggtt ggctcatcca aaaaaagaat atcgggttgt gtcatcagcc acttggataa
     4381 aacgactttt tgttgattcc caccactaag actactgaca atttgatgga tggagtttgt
     4441 ttttatacgc atctttttcc ggaaattttc tgcctcagtt acttcttttt ctttatctaa
     4501 tactcctttt tgacttattt tatccaaact gctcagcgtc acgttttccc gaatatccat
     4561 cagcagattc agtcctaaag attttcggtc ctccgagaca taagcgagtc cgtttgcaat
     4621 ggctgaagga acggtcttaa ctgatatctc tgccccttcc tttaatatac gtccggatat
     4681 cttgcttcca taagaatgtc caaatagact catagcgaat tcagttctgc ccgctcccat
     4741 cagtccagct attcctacaa tctctccttt tttcaaatgc aagttgatgt ggtcgtttat
     4801 gattcgctcg tgatcaagag ggtgatagac cgtccagtct tctactttaa aataaacttc
     4861 tccaatatcc ggttttcttt caggataacg atcaactaaa tctctgccca ccattccgcg
     4921 aataatatgc tcttcgctga tcatttcatt ttctagtgtc tcaatcgtct gtccatcccg
     4981 taaaatggtg attcgatctg cgatatccac gatttcattc agcttatgtg aaataatgat
     5041 ggaggttaca ccttgctctt tcaactctcg gatcaatctc agcaaattcg cgctttcttc
     5101 ttcattcaat gcagcggtag gctcatccaa aatcaacaga cggacggatt ttgataatgc
     5161 tttagctatt tcaactagtt gctgttgccc tacgccaatc tgggatacta gaagattcgg
     5221 gtcgatagac aaaccgactt tattcagcag ttcatatgtt ttcttttctg tcagttccca
     5281 atcaatcacg cctctttctg cttgttcatt tccgagaaaa atattctctt ttattgaaag
     5341 ataaggactt agtgctaatt cttgatgaat gatgacgatg cctttttctt cactatcttt
     5401 caaatcttta aattgacaca gctttccatc ataaaagatt tccccgtcat aggagccata
     5461 tggatacaag ccgctcaaga cattcatcaa tgttgatttt cccgctccgt tttctccgca
     5521 caatgcatgg atttccccac gttcaatcat cagattcacg caatctaacg ctcggattcc
     5581 agaaaatttt ttggtgatat ctgccatttt taaaatacaa tccgccataa ttcttatcct
     5641 ctctctgact tagatctctt tagttccttg tttggttcct cttaattttt cagatcggat
     5701 tctttataat aatcggtgtc gatcagctct gcttgataat tatctttatc gacagatact
     5761 gtatttgcta aatacgtagg gacagttttc accccattat cataagtttc agtatcattg
     5821 accggaacct cttctccatc actgattgct ttgatcattt caatcgtatt ttttgctaag
     5881 atacgtgtgt ctttaaagat cgtttgtgtt tgttccccgg caatgatcga tttaacgcca
     5941 gcaactgtcg catcttgtcc agtaatcaca ggtaaaggtt tactttcact cccatacccc
     6001 acgcctttta atgacgaaat gattcctaaa cttattggat catatggcga caaaacagca
     6061 tccaatgttt ggtcagtata atttgcgctt aacagattat ccatccttgc ttgcgccgta
     6121 gaaccatccc agcgaagcgt agcgatttga ctgaaactcg tctgtccaga tggcacgacc
     6181 aactgaccgt tgtccatata tggttgtaaa acagacatca ctccgttgta attgattagt
     6241 gcgttgttgt cgtcaggcga gccaccaaat aattcaatcg tgaatggtcc ttttccttct
     6301 ttcaatccta agtgctcttc aatataagct gcttgcgata cacctactcc aaaattatca
     6361 aaagtcgcgt aataatcgac attttctgaa ttcatcagta aacgatcata ggcgatcacc
     6421 ttgatatctg cttcttttgc tttagctaac acatcagtta aagccgaacc atcgatagaa
     6481 gcaatcacaa gtgtatctac cccttttgta atcatatttt ctatttgtgc tacttgattt
     6541 tctactttat cttctccata ttgaagatct gttttataac ccagtttttc taattccgag
     6601 accatgttgt tcccatcagc gatccatctt tctgctgatt tggtcggcat agcgattcct
     6661 acataccccg tactatcatc tgcagatcct gaattgccac acgcacttaa tagaatgctt
     6721 gcggtcaaaa tagcgatagt cccaacaatc tttctaaatt tgtttttcat tttttatccc
     6781 ccatttcaat tgatacaccg agtatagcta ataaagaaag cgttaacatt gattttttta
     6841 tgggatttac tttaaatttt ttaggtgtac tttttttgtc ttggattttt ttaagtttct
     6901 tggattttt
//
LOCUS       DAGCWU010000139         6879 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.140, whole genome shotgun sequence.
ACCESSION   DAGCWU010000139 DAGCWU010000000
VERSION     DAGCWU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.140"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            124..981
                     /locus_tag="MBZ71_002265"
     CDS             124..981
                     /locus_tag="MBZ71_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBT4430182.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            974..1876
                     /locus_tag="MBZ71_002266"
     CDS             974..1876
                     /locus_tag="MBZ71_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBT4430183.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1909..2259
                     /locus_tag="MBZ71_002267"
     CDS             1909..2259
                     /locus_tag="MBZ71_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4430184.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2308..3230
                     /locus_tag="MBZ71_002268"
                     /pseudo
     CDS             2308..3230
                     /locus_tag="MBZ71_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            3308..4201
                     /locus_tag="MBZ71_002269"
     CDS             3308..4201
                     /locus_tag="MBZ71_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4430185.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4239..4802
                     /gene="amaP"
                     /locus_tag="MBZ71_002270"
     CDS             4239..4802
                     /gene="amaP"
                     /locus_tag="MBZ71_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBT4430186.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4816..5004
                     /locus_tag="MBZ71_002271"
     CDS             4816..5004
                     /locus_tag="MBZ71_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBT4430187.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5017..5919
                     /locus_tag="MBZ71_002272"
     CDS             5017..5919
                     /locus_tag="MBZ71_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4430188.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5951..6193
                     /locus_tag="MBZ71_002273"
     CDS             5951..6193
                     /locus_tag="MBZ71_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4430189.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6449..6820)
                     /locus_tag="MBZ71_002274"
     CDS             complement(6449..6820)
                     /locus_tag="MBZ71_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430190.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2506 a         1172 c         1375 g         1826 t
ORIGIN      
        1 ttgtgataaa aaaattttaa aatattcaat gaaaacaaag catatatttt gctttaaaac
       61 caaatgagga atattctaga tgtagaacag gttagaaaaa gattagaaaa aggagtgatt
      121 ttgatggaga agagatacgg cgtagttcct gtggtaactg cttcagacga aaattatgct
      181 ccttacttga gcgtgatgat cgcaactgca ttagaaaatt gcaataaaac aagacgcata
      241 aaattttatg tgattgacga tggtctatca gaatatagta aagaaggatt ggaagaaacg
      301 gtaaataaat attcaagtaa tgcaagtatc caattcctga cagtggaaaa agatatctac
      361 gaagacttcc tagtcagtga ccatatcaca actaccgctt acttaagaat ttctttacca
      421 aacttattag ctaaagagga ttataagaaa gtcctttact tagattcaga tgtgttagta
      481 ctagacgata tcgtaaaact atatgatgag ccgttaaatg gaaaaacaat cggcgctatt
      541 atcgatccag gccaagtgaa agctttggaa agattaggaa tcgattccga tgacttgtac
      601 ttcaattcag gagtgatggt gattgacatt gatcaatgga ataaaaaaga aattacagaa
      661 aaaactatcc attacctcag tgaaaatggc gatcgaatca tctatcacga ccaagatgct
      721 ttgaatgctg tactttatga agattgggaa caacttcatc caaaatggaa catgcagact
      781 tcgctgattt ttgaacgtca tccagcacca aatgaaaaat atgaacgtca gtataaagaa
      841 ggaaatgaaa aaccttcgat cgttcacttt acaggacatg ataaaccttg gaatacgttg
      901 aaagatcatc cgtataccaa tttatactta aaaaaattag cacacagtac tctaacgaaa
      961 gtaggcgaga tcaatgaata aaaaagaaat agcagtagtc gcaagttgta acacaaaatt
     1021 cgtaccacat ttagctgcat tatttgtctc agtattagat aattgcaacc cgtctaaatt
     1081 tgttcggttc tatgtgattg acgatgatat cgattttgaa agtaaacagc ttttacgctt
     1141 ctcagtgaaa aacgcacgaa tgaactcaga tgtggaattc ttgaaaatca ataaagaatt
     1201 ctttacaaat gtggtaatca gcgatcgtat tccagaaaca gcttattatc gtattgcgat
     1261 tccggaattg ttccgcggca cagaagttga acgtatctta tatatggatt gcgacatgat
     1321 cgctttacaa gatatctcta aattatggcg tcttgacttc ggtgattcaa tcgtagcagc
     1381 tgttgaagac gctgggttcc atcaacgact ggaaaaaatg aaaatttcag ctaaatcaat
     1441 gcgttatttc aactcaggtt tgatgctgat caatgtgaaa aaatggttag atgaaaatat
     1501 tacgcaaaaa gtattagatt tcatcgaaca taatccagaa aaattacgat tccatgacca
     1561 agatgcatta aatgctatct tacatgaccg ttggttacca cttcatccgc gttggaatgc
     1621 acaaggctat atcatggcaa aagcaaaaaa acatccaaca gcagcaggtg aacgtgaata
     1681 cgaagaaact cgcaacaatc cttacattat ccatttttca ggacatgtga aaccgtggag
     1741 caaggatttt gaagggccaa cgaagaaata ttacgaaaaa tatgcaggga tgacggcttt
     1801 ccgttgtgta gctaaattcc ctaaatatcc aaaatatgca aaagttcaac caaaaacaga
     1861 tgtagttgtt aaataataga agacaacaac tgaagagggg gagtaggaat ggaaattaca
     1921 atgtctaaaa atgcagtaga gacgttaatt gaaaaggttg gggaaaatac aaaaatcgca
     1981 ctcgcgctta tcaatgattc cgatccattt ctaagggaca aaggtgcttt tgcaaaaggc
     2041 agcttttttc aaatcattcc ttttgtttcg gaatttggag aatatgcgac taaaatcgag
     2101 catccattat tggacatcta tacatctaaa ttagagcaaa attatttcgg taaacgattg
     2161 aacatggact tcaacaaaca gcttgacagc ttttcattag aaaatgaaat cgcagtactc
     2221 gattataata tcaaattaaa gaactgcttc ttcagctaat agtttcgaga aagtccaaaa
     2281 aggaggtata cattgagata ctactttgtg gatgaagaaa agaatgtaat agaaacagat
     2341 gaaaaaaaat ataattggct cgtacttgat agtgcaaagc ccgaagagat tgagcaagca
     2401 atcgaagaat tcgagcttcc tgatgatatt ttcgtaggaa ccgaatatcc ggaagaagtt
     2461 tctcggatgg agcgccttta tgagacgaaa ctagagcatc cattttcttt ggtagtgatt
     2521 aatctagatc agtcagataa tcgtatcgaa aaaaattaac gccaatatct tttgtacttt
     2581 ccgataatct cttgattact tgtatcgata gaaaaaccga atttgtggat cggctgctga
     2641 caaagcatgg aaatcattta aactcgtttg aaaagatcat cacgtataca ttattagata
     2701 tttacaccca ttacgtgaaa gaactaagag agatgaaaaa gcgaattgac gctttggata
     2761 aagcagctcg taaaacaacg gaaaatgaag aactgtataa acaggcagat cttgaaagag
     2821 acatcgtata tatcgatcat accttaagag atcaacggga aacaatggat caattatgga
     2881 attctgaaac tttcatcaaa cgtttagacg atgaacagct actctatgat gtccatttga
     2941 gacagcgaca aacagaaaaa atgatcgaga tctatcgaga tttattagag agtattggtg
     3001 atctattcaa cgggatgatg gacaataatc taaatcatat catgaaatat ctagattcag
     3061 ctgcattaat catttccgtt ccggcgttgt tcgcagggat ttggggaatg aatacaggag
     3121 gattaccggg taaaagttct gctctaggct ttatactcgt agtctttggc tctttgatag
     3181 cagcagtagt tttaggttac catttatacc ggaaagatta tacaaattaa agaatctatt
     3241 taaaacaggt atcaaacaaa gaaatttgtt ttggcaatac aatttaaaaa ctaggaggaa
     3301 tacagcaatg acagaaccaa aattaatcga cccgcgaaac ttgtaccata cggagaaatt
     3361 tcctaaacaa gatcaggata caccagcttt acagcagaat atgattcctg ttcctgattg
     3421 tggagaagaa tcctatgaag gcaataatca actagaaaac cgccgtgtac taatcactgg
     3481 tggagattca gggatcggtc gtgcagcagc gattgcattt gctagagaag gggcagatat
     3541 cgctcttcaa ttcttcccag gagaagaaaa agatgcagag gaagtagcaa aatacgtgaa
     3601 agatgcagga cgtaagattg tcttactacc ttttgatctt cgtgacgaac aagcacctaa
     3661 agaaatcata gataaagccg tgagtgaact tggcgggcta gatacgatgg tactaaacgc
     3721 aggacagcaa atttcttgtc agtctattgc tgagttacca cttgagcaag tcaaagacac
     3781 atttatgata aacatcatcg ctatgtttgc tttagtgaaa gaagctgttc cgcatatccc
     3841 agcaggagga gcaatcgtaa caacaacttc tgttcaagca ttcaatccaa gcgaacattt
     3901 attagattac gcagcaacaa aagcttcaat cgctaacttt acgatcggtt tagcaaaaca
     3961 gcttgcacct aaaggaatcc gtgtaaatgg tgtagcacct ggtccaatct ggacaccatt
     4021 acaactagac gaaggacaac cttctgattc gatccctgaa tttggtcagc aaagtttact
     4081 agaacgtgca ggtcagccag gagaattagc accagtttat gttttcttag catcgaataa
     4141 agcaagttat gttacagccc aaatttacgg agtaactggt ggagaagcga tcaacttata
     4201 agaagttgat cgtttcaccc ctatgaggag gtgcgaaaat gaataaaggt gtaaaaacga
     4261 tcggagtgat cggaacatta cttttgctat ctgtgctttt gtttgcgata ctattaagtg
     4321 caacctatcc attgcctttt cagctagaga agtttcgatt cttttccatc actaattcat
     4381 acgtgcaaca atatgtattt tggcttgccg ttgcatttgc tattttgaca attatcacgt
     4441 tacttgtttt cctgttttat ccaaaaacaa tcggaaactt tctactgaaa catggtgatg
     4501 gggagttgac gttggataaa aaagcaattg aaggaatggt tcgttctcac ttgcatgaag
     4561 atgaattcat ccattcacca aaagtaaatg tcaaagcaac gaaaaacaag atcgccgtca
     4621 atgtaaaagg ggagttgaaa cgaacttcag cactcattgg taaaacagga acattgatga
     4681 aagaaatcga aaatgaagta caacacatat tgggaacaaa agagccagtg aaagtatcgg
     4741 tgaaatacac aggctttgaa gaaccagcta aacgaagcaa tgaccgcaaa cgagtagaat
     4801 agggggggac caaaaatgaa agaactattt tcgacataca agctccctat catctttggg
     4861 gtacttggat tgatattagc cgttttatgg atcaccgtgg gctttttcaa gaccttattg
     4921 atcttgatca tggcagcgat cggcgtcgct atcggttttt atctgaaaca aacgaaaatc
     4981 ttagatcact attttaaatc ttaggaggaa acatccatga ataacaataa caaaaataat
     5041 aacaataaaa tgaacaacca aaaaccagtg tctgcacatc cagacccaac gacaccaaac
     5101 caatcaaatg gtgtgaacga tgaaaagaac aaaacagcac cagtatctgc acatccagat
     5161 ccagtagtcc ctaacgttca acaaggagta gaagagaaga aacatgatgc agaaaagcat
     5221 gcacctcatc atccaggaac tccagaatct aaagttccag aacaacatga tgcaatcgat
     5281 ccttctaaac ctgtggaaga gcaaaaacat ccttctgacc atgcgaaagc acaagaaaaa
     5341 cttagttctg accacacaaa cggacaagga aaacttggtt ctgatcatac aaataaccaa
     5401 ggaaagcatg attctgaaca ggaagattca atgcgcggtg agctgaatta tgaagataaa
     5461 gttgtgcaaa aaatcatcgg aatcgcaatt gaacaagtag acggtttatt atctgcaaac
     5521 ggtggtttct tctctaatgt ggcaggaaaa cttgtgaata cagataatgt aacggctggg
     5581 atcgaaacag aagtcggtaa aaaacaagta gcagttgata tggatgtcat tgtggaatat
     5641 ggcaaagata tcgaaaaaat ctttgaagaa atgcaagaag taatcggcaa agaagtaaaa
     5701 aatatgacac atttagaagt gatcgaagtc aacgctaacg tagtggacat caaaacaaaa
     5761 gaagaatttg aaaaagatca agaaacggtg caagacaaag taactgacgc agcgaaaaaa
     5821 accggtgaat ttgcttcgaa tcaaacagat aaagtaaaat cagcagctgg aagcggcgca
     5881 caaaaagtaa aagaaaatac agaaccacgg gttcagtaat aaggaagtaa aacattagga
     5941 ggaacgaaac atgggattta tttggtcact tatcgtcgga ggaattatcg gagcaattgc
     6001 aggagcaatc acgaataaag gttcttcgat ggggattatt gcaaatgtcg tagcaggttt
     6061 agttggatca gcacttggtc aagcattatt aggttcttgg ggaccaagta ttgcaggaat
     6121 ggcaatcgtg ccatcattga ttggtgccat cattttagta ctaatcgtat cattctttgc
     6181 tcgaaaactg taaaaagact tttaaaaaga ggtcgtgaaa aagctgttct tcatcaagta
     6241 ataagaacag ccaaacaaaa atagcttctc atatttttca tttggttggg catattatcg
     6301 aagatgcaag cttttgaaca ctgtttatac ggaataagag gctgtgacaa gacttttgtc
     6361 acagcctctt atttatgcgg gcaaattccc aattagattt ttaaaagttt ttccttaaat
     6421 gtgtctttat tgatacagag aagcttcttt aataaaacaa tgcctcccta aattttcttg
     6481 agataggaaa ggtgaacata caaaataata cgaccacttt gaaaacccag atgatccaca
     6541 aatggatcgt atgatactga tgaagtaaaa agcaatggat cagaaactca aagagattga
     6601 aaaagacaag gaagaaataa tatctctttt ttactcttgt ctgttttagc caataatagc
     6661 tcgtaaagta atctagacaa acaaatagaa gtaaacaaag tttcgtaaga gcagcaatcg
     6721 tatcatgttg ctctgctaga cgattgacga gcaggaaaaa tgttggtaat ggaatataga
     6781 tcacgagtaa tgaaagaaaa aagtaaacaa taaatggcat actattcctc ctaattgtgt
     6841 gagttcgccc tcattttaaa ataacgatat ttgtttgtg
//
LOCUS       DAGCWU010000140         6694 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.141, whole genome shotgun sequence.
ACCESSION   DAGCWU010000140 DAGCWU010000000
VERSION     DAGCWU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.141"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            117..1064
                     /locus_tag="MBZ71_002275"
     CDS             117..1064
                     /locus_tag="MBZ71_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBT4430191.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            complement(1361..1909)
                     /locus_tag="MBZ71_002276"
     CDS             complement(1361..1909)
                     /locus_tag="MBZ71_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430192.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1910..2767)
                     /locus_tag="MBZ71_002277"
     CDS             complement(1910..2767)
                     /locus_tag="MBZ71_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4430193.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(3053..3340)
                     /locus_tag="MBZ71_002278"
     CDS             complement(3053..3340)
                     /locus_tag="MBZ71_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBT4430194.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3330..3659)
                     /locus_tag="MBZ71_002279"
     CDS             complement(3330..3659)
                     /locus_tag="MBZ71_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4430195.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(3736..3825)
                     /locus_tag="MBZ71_002280"
                     /pseudo
     CDS             complement(3736..3825)
                     /locus_tag="MBZ71_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            3900..4580
                     /locus_tag="MBZ71_002281"
     CDS             3900..4580
                     /locus_tag="MBZ71_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HBT4430196.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
     gene            4608..5171
                     /locus_tag="MBZ71_002282"
     CDS             4608..5171
                     /locus_tag="MBZ71_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4430197.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(5216..5383)
                     /locus_tag="MBZ71_002283"
     CDS             complement(5216..5383)
                     /locus_tag="MBZ71_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4430198.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5417..5716)
                     /locus_tag="MBZ71_002284"
     CDS             complement(5417..5716)
                     /locus_tag="MBZ71_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBT4430199.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            complement(5757..6374)
                     /locus_tag="MBZ71_002285"
     CDS             complement(5757..6374)
                     /locus_tag="MBZ71_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4430200.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
BASE COUNT         2085 a         1238 c         1039 g         2332 t
ORIGIN      
        1 tcattttaaa ttttaaaaaa atgatgctta tttttaaaaa tttaaaaaaa gactaaattc
       61 aatttttttg gtataatgag aaaaaatatc tatttgaaaa agttaggagc tatactatgc
      121 atctggaatt ctctgactat gaatcactcc ttgtaaaaat ttgtgaagac tattatctcg
      181 aggatatcaa tattagtgaa atttctaaaa aatatgggat caccagatat aaagctttaa
      241 aatatattga agatgcacgt gaaaaacaat tagttagtat ttcaattaat tctccgtatg
      301 ctaggaatta tgagctagaa gaaatcttca aaaattactt taaaacgaat atctatatat
      361 taaaaagtag tgaagactta tcacaacacg atttattatt ttcaaaattt gctgccgatt
      421 acattcaaga attaattaag aaagccaatg tggttgcact atcttgggga gataccgttt
      481 acaaagtgat tgatcagttt aaaatgggtg ctcgtgaaga attaattttt actcaattca
      541 ttggcgaaat cggaaaatat aattccttag ctggctccac acgattagtt caaaaagctg
      601 ctgaacgtta tgagtcttct tatttgactc tcagtattcc tttatatgtc atcaatgaaa
      661 ctgcacgtga actaatggct ctagaacctt ctttagctaa taccttagca accgcttcgc
      721 atgcagatat gttaatttct ggaattggca ccttatcctc tattgattct gtcgaaccct
      781 ggaacaagca tcgtaaaatc ctatttccaa gcatgacgga tactgttgga tttctttacg
      841 gaagaccctt caatatcaat ggtgaattca tcaaagcatc tgaggataag acttttggtc
      901 tttctttaaa agaaattttc gatattccaa aacgaattgg tatttgtaaa agtaagttca
      961 aagcggagag ttgtttaggc gccttgaatg gtaatttttt tacagattta tttttggatg
     1021 aaaaaactgc ttggaaaata ctttctttga taaaaaaaga gtgagtcatg taaaatcctc
     1081 caaaaaattt agatttttgg tactactttt ttggagggca gtacaatgat tcactctttt
     1141 tattggatat caaaatccct ttttttgaga agcttgtctt gatatttttt gaacttttcc
     1201 aagccatact ccattacttt tcaagattta atttctttgt attcactgaa taatttccac
     1261 gttttttcgt taatgttact tcttattaat tttattgtac ttctcatgca ataggatcat
     1321 atatccttct aaatcttttt tatgctataa aaagggggtt ctatctaact ttattattca
     1381 ttccccaccc tttgcgcagt gagtcttcgg tatcttgttt tatctgactg gtagttctta
     1441 ttatttctcc acggtcatct aattctggaa caattggata tttttctctg aacaagtcta
     1501 gttctttcac cacgtcatta aacaaatctt gcttatcttc ttgcgtaaga ttctgtttaa
     1561 tatactcatc taaagctttt ctaagcaatt catctcttgt catattagtc tcatactttt
     1621 ctactaaaac tctggaagtc gcatttaaca aagtcgacag ttttaaagga tgcttggtgg
     1681 aataaacctt atcaaacttt ctgattggtg cgccaacttt ttttgttctt cctctactct
     1741 gtctttcaac attggaggaa gtttttattt cttgaacttg gtgcacttct aattgcttat
     1801 tttcagatgg tgtaattgct tgctgctttt tctttgcttc tgcttctttt tttattttgg
     1861 cgagtccttt gttgttccca aaaatatcaa gtcctttgat gtcagccaat tactccacct
     1921 ccgcatacca attctcgtgt tcttccaatt ctttcaaaat actgataaat aattcgtggc
     1981 ttttgcgatc ccattgtttt aagagacctt tctttgtctt ctccatcgta atacctgatc
     2041 cgtcgtatcg ttttaagcgc gcatttttga gtacaacatt tttaatcaaa tgttccccat
     2101 aaatctcttg ggcttgttta tacatttcct gatctactgc gtcaccgctt tccaacatga
     2161 aaggaataac accagcaaca tataaatttg tatcaaattc tttcacaaaa aagtccatat
     2221 actcaagata ttgcatcgca tttctcatcg attttacttg ggtctgtaag acgacgattg
     2281 cccagtccgc aatgtacatc gcggatttac tatatacaga aatagaaggt gggacatcta
     2341 aatagatgac gtcaaactca tcaaataatg gttctagctt ttctttcata tatttgaatt
     2401 gggataatga atcattatat tggtctttga tccaatcatc atacatttca aaggacatat
     2461 ctgctggaag tatatagagg ttattgatta ctttatagac agcctcttta ggtggaagat
     2521 tgtttatcaa cacgtcagtc acagtatttt cgtaaacaat cggttcacct tctccgcctc
     2581 cttcttcgtt agtgaagttc atatgcgtta agttcatcac atcagaagta tttccttgca
     2641 tatccaagtc aattatcaag gttctttttc cttcattaac tgctgattca aaccctaaca
     2701 tttgcgcgca cgtactttta cctacgccac ctttaaaatt accaattaca tatttcttcg
     2761 ctaccacgtt cctacctctt ttcttttagc aatattcatt tgatactatt ataacatatt
     2821 tatttttagt tactaccaaa aaaaagatat atatgtacta ccttataatt aataagtaaa
     2881 aggtagtaca taaataagga aaagttgaaa tatattgatt tatagctttc ctcaaagtaa
     2941 tgacgttatt taatttatcg agaagcagag aatactcgcg actttagtca tgtagacgaa
     3001 tcagattttc gataaaaaag aagccgtcct tcttaagaac gacaacaaat cactagatta
     3061 attttttcaa ctgttcataa aagttttcat gcgttccaca taacaatact ggaataacct
     3121 tgttttctat aatctcgtaa gctactctat atgttgtgcc cgcatatttg aacccactag
     3181 accaaatacc tgataaatcc ccagtttttt gttcaccctt atatggatca atcgcaattt
     3241 catcataaat taaattcaaa aatttttctt tcaatggctt ctcttttagc ttttttaaat
     3301 acttaatgac atgttttgaa ggaacgattt cataagccaa tttctgttgc caactcctct
     3361 ttagtcatta ctttagaatt agtaagtgta tcttcagcaa tatctctaaa ggaagaatgc
     3421 attttgtttt ttctattctt aaattctaca agaatctcat ctttatcgta gccttctgca
     3481 attatatcag acaaaatatc ttcgctaaaa tcataaaatt ctttttgtgg ttcaacaaat
     3541 tcataaagaa taccattctc taccaacttt gcttcaactt cgactccatt aggaacatta
     3601 aaatctttag gtactgtcaa catgattgaa ttgccttgtt ttcttgtttt tgtaatcatt
     3661 taaatccctc atttctaaca gtatgtaatt acttagtaaa tacattatac cacgagataa
     3721 tgtatttaca agtaattact gtgactcgta atgcgtcctt gctgaataaa tttcaaccac
     3781 gtgttcatct tcatctacct tataaacaac ccggtgttgt ctattcggtt ctgttgcaaa
     3841 gttttccaaa aaatctattt tagtgtaaaa ttgagaaaaa agacagagag gacagagtaa
     3901 tgaatcattt taaaggcaaa caattcaaaa aagacgtcat tattgtcgct gttggttact
     3961 acctgcgtta caatctaagc tatcgtgaag ttcaggaatt gttatatgat cgtggaataa
     4021 atgtttgtca tactacgatt tatcgttggg tgcaagagta cagcaaagtc ctctattatc
     4081 tttggaagaa gaaaaataga caatccttct attcatggaa aatggacgaa acctatatca
     4141 aaattaaggg acgttggcat tatctttatc gtgcaattga tgcggacggc ttaaccttag
     4201 atatctggtt acgaaagaaa cgggatacgc aagcagccta tgctttctta aaacgactcc
     4261 ataaacagtt tggtgagccg aaagcaattg tgaccgataa agcaccttct cttggctccg
     4321 cctttagaaa gttacagagt gtgggtttat atactaagac agagcaccga actgtgaagt
     4381 atcttaacaa tttaatagaa caagaccatc gacctattaa acgacgaaat aaattttatc
     4441 aaagtctccg tacagcctct tccacgatta agggcatgga gaccattcga ggaatatata
     4501 aaaagaaccg aagaaatgga acgctcttcg gcttttcggt gtctactgaa atcaaggtat
     4561 taatgggaat aacagcctaa gatatttgga gttcagagag ggcgcgtttg attttcaaac
     4621 ttcgcaacag aaccgaagga cttaacattg ataaattatt ccaagaaaaa gccagtggta
     4681 aagatactga gcgtcctgag tttcaaaaat tactaaatta tatccgtgaa ggtgattgcg
     4741 tcatcgttac atcactagat cgtttaggaa gagattatga agatataaaa aatacagttg
     4801 cttttatgaa acagaaaaaa gtagcgttaa aaatattaga tgcacagttt ttagatttta
     4861 atactggaaa tgaactcctg gataccgcta tgttcgatat gtttttatca ctgctaagtt
     4921 atattgcaca aaacgagcgt gagaaaattc gtgaacgtca gcggcaagga gtattattgg
     4981 ctcaaaaagc cggccgatat aagggacgac caatcgagta ttctattaac tcaacagatc
     5041 cgcaaaaacg cttagttttt aaaaccgtgg tggacatgtt aaagcaaggc attccagttg
     5101 cagagattgc aaaagaaaac gggatctctc gaccaactat ttataaaata aagaaagcga
     5161 atgatttgta gaaatatact acattcattc gcttttctac cgcactattt cgtttctatg
     5221 ttccatgtac cccgtatcgg cgatggcatt taggacagta aaagtaattt ttttgataaa
     5281 cactttttgc taattcccca cagtcacact ccacgtgaaa agcaatattt cgattaaaac
     5341 tagttatttc aatgccccaa cgatcataag attttcgctt cataaaaatt ttcctccttg
     5401 taaattttac taacgattat aaataccgtt gccattcttt caactgttga ctaattaacg
     5461 cataaaattc gacttctgat aaaatggtaa tctcatgacc taacgcaatc aagtcccgcg
     5521 cggtttgttc cttttttgta tagagagaat catcaaacaa cgacttagaa tactgaccac
     5581 aaaccaggaa atttgttttt gaagtaacac ttgattgaca atggccgttt aaagagtgaa
     5641 ccaaaccgat ggcttgggtt cgtgtcattt gctgcagcgt tccagtaaaa acgacccttt
     5701 gattcgttaa gtccattttc aaactctccc ttcccgtcta atagctgtta tatctatcaa
     5761 gaaaagtggt gcaaggtatg ttcatattct ttcacccgtc gataaaaggt atttcgtttc
     5821 atgcccaatt gatccatagc atagactgca gttatctttc ctgctttcca gtcagaatag
     5881 atcgcaggaa attcaggagg taaagcaatt ttctttctgc cgaattttac acccttttgt
     5941 ttcgcagctt ctacgccttc tctttgccgg ctattaattc gtttccgctc gtcttcggcc
     6001 atatagctga gtaattgcag aattaagtca gagataagcg tctctaaccc atctagttgc
     6061 ttgtaactag cagtatctaa cagaggcatg tctaacacag aaatatcaat ctgcttttcc
     6121 ttagttaatt ctcgccattc ttctaaaatc atttgtttat ttctgcctag tctatctaat
     6181 gattgtagat agagtaaatc tccttttcga aacatccgtt tcattaattg atattcaggg
     6241 cgtttaaaat ctttgccgct ggctttttct acaaatatat attcttcagg gattcctaat
     6301 tccttcgcgg aaatcagttg tcgttcgatc gattgatcat aactactgac ccggatataa
     6361 aaaaattttc tcatcgtttt tatctaccct tttttcacta aaattgtttc aaaacaatca
     6421 gattaaatca gaaacgtttc aaattgtatt atgtggttat tatgccatac ataaacaaag
     6481 aaaatcaagg catgtcaaaa agtatacttt ttgaaacagt aagtttacac tcccgcatga
     6541 ttatatttat tccgctaaaa tacataaaaa tggcaggcct taacggtctg ccattaaata
     6601 aactagttat ccagtttttc ttttatgtct tccatggtat attttatttt tcacatcaga
     6661 atttgacttc tttaactttt acaatttttt taac
//
LOCUS       DAGCWU010000141         6434 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.142, whole genome shotgun sequence.
ACCESSION   DAGCWU010000141 DAGCWU010000000
VERSION     DAGCWU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.142"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(99..1229)
                     /locus_tag="MBZ71_002286"
     CDS             complement(99..1229)
                     /locus_tag="MBZ71_002286"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBT4430201.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="MBZ71_002287"
     CDS             complement(1878..3080)
                     /locus_tag="MBZ71_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4430202.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="MBZ71_002288"
     CDS             complement(3140..3343)
                     /locus_tag="MBZ71_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBT4430203.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="MBZ71_002289"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="MBZ71_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="MBZ71_002290"
     CDS             4880..5692
                     /locus_tag="MBZ71_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4430204.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1930 a         1314 c         1008 g         2182 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcataga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatattttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cact
//
LOCUS       DAGCWU010000142         6384 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.143, whole genome shotgun sequence.
ACCESSION   DAGCWU010000142 DAGCWU010000000
VERSION     DAGCWU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.143"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(50..277)
                     /locus_tag="MBZ71_002291"
     CDS             complement(50..277)
                     /locus_tag="MBZ71_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430205.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(444..2414)
                     /locus_tag="MBZ71_002292"
     CDS             complement(444..2414)
                     /locus_tag="MBZ71_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430206.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2429..3295)
                     /locus_tag="MBZ71_002293"
     CDS             complement(2429..3295)
                     /locus_tag="MBZ71_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430207.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3285..3518)
                     /locus_tag="MBZ71_002294"
     CDS             complement(3285..3518)
                     /locus_tag="MBZ71_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4430208.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3530..5686)
                     /locus_tag="MBZ71_002295"
     CDS             complement(3530..5686)
                     /locus_tag="MBZ71_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430209.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5706..5927)
                     /locus_tag="MBZ71_002296"
     CDS             complement(5706..5927)
                     /locus_tag="MBZ71_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430210.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(6037..6375)
                     /locus_tag="MBZ71_002297"
     CDS             complement(6037..6375)
                     /locus_tag="MBZ71_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430211.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
BASE COUNT         1799 a         1260 c          875 g         2450 t
ORIGIN      
        1 ctgagatcac ctttcaatct aaatacacgg ttcttttctg gtaacttatt catgcttcaa
       61 ccacctcata ctgttctctc atacctttga cttcattaaa aaaaactctc gctttttctt
      121 catcagtaaa gtttttatac aactgattac aataatgagg tttttgaagt ttctgaatac
      181 ctatactaac ttcaaatcct tcttcatgtt gaattaggat tacttctgtg tctctaccta
      241 gaaatattcg atctaaaatt tttatgtttg gtttcattat ttaacctcca ctataattat
      301 ttcactcaat aaggtttcac tttatgaaat ttctatagtt ctcttttcac aaaacgtacc
      361 atgttctcta agactgtttt tagtctcaaa tatacatgtt ttttcttatc ctttccttat
      421 acactcaaaa tcaaagataa aacttaaaga ataaactctc caataacaaa attattagtc
      481 agatccactg gtctcctgat ctgaaattta ttacttcttc ctgttgtcaa atactgtctc
      541 aaatcaacag aaagtttttg atcatcttct ccttttctag caggagcaac ttttttttca
      601 tacttcatta actctccact ggtcatcttt ccaatgatag tgtaattaaa atcactttca
      661 aaactttggt aaatttttcc atctgtattg aatatatcag ctgtttcaat atcttctttg
      721 tattcattgt cttctcctga accgattgta tcaaatagaa aggcaagacg aattccatct
      781 tcttcaagca ttgttaatct ttttttaatt agcccaacgg tttcaataga taacttatcg
      841 aagccgtgaa tcataacaat atcatctttt tttgcttgtg atgtgatgaa atcaaaggta
      901 tttaaaaatt gagcttccat aacattggtt tctgctctta gatcacctaa gttatagtaa
      961 aactgtaatt tgtcactttt accgatttct ttcagtgtcg ttctttgatt aaacatccta
     1021 gagaagctgt ctaatgctgt tctcaatgtg tcttttaata caataatacg atcgacttct
     1081 ttttcagtac cagatttttt ggcttttgtt tcagcattag tcaaatagct aataaaatcc
     1141 ccaagaactg gcattgtatc actactgttc accaaaagtt ttgaacgttc ttcataatta
     1201 atatcccaaa gtttctgact tatataaaag ttacctaatt cttttttcaa taaaactcta
     1261 gttgcagaat ctaatgttct gcctgctaac aaataaaaaa tctgagttag tttatcgaga
     1321 ttccgtctat ataaatcacc aatgtccttt tgattaccaa aaagctccaa agagttaaga
     1381 ccttcttcag ccaaattaac cttttctatt acactgttta acgaaggttt ggcaacaaat
     1441 gttctaccag ttggatcacc aaagtaatca aatttattta agacaatatg aaaagttcgt
     1501 ttatgatttg ccattgcatt attagcaatc aattgccccc aaagtgatga tgctgagatt
     1561 ttttcatcat agcctttaat tgtagcgttt ttgtgtgatg ccactaaaac tttacttgta
     1621 aaagaatcat ccagtgccat aaaagctaca ccacttgcgt agctagtaga catagtgccg
     1681 atagcccatc cattcgcatc atctaaacct ttgcgaattg catgatcatt tcctgcaaaa
     1741 tccgaactca tagacgtata ctcatagaca ttcccgattg gttcacttaa aagtctatca
     1801 aacagatcac gttgatctcc tccatatgac ataagcttta atccatacat tttttcatca
     1861 tacaaccgtt ggagtttaat tagctgttcg ctgattttat taggagaatc ggatactaat
     1921 aggatttcta gcttccaatc aataatttta tttgccatgc tgtctagtaa agatgctgta
     1981 ttcaaatcca taatccgcat aacagttttg ctaatttcag catctgaatt ttttccttct
     2041 aaattatttt cataactatt cgttgatccc ccaacattct cgtgcattat ttcttgttga
     2101 tctttttgtg acactggctt atctgcttca attagatagg cttttacccc ttctactctc
     2161 acttcaggaa tagctttaac catccaacca aatccataat ttctattcat tccataagta
     2221 ttcactaatt tcacaattgt tccccaccgc ttacctgtcc taatgtaaga tggtgtaaat
     2281 tgataaccaa ctaaagatgt taaatcacta acaaaggtat tattcactac ctttgaattt
     2341 ctagtttttt gttctttttt agtttttctc ttttttttga aatttaatag ttgtgatcta
     2401 cccataattt ccatttaatc tctcacctct atttcagtgc ataaatatca gataatattt
     2461 cttttgtttg tttagctgac aactgagtga cactataata aagtcctcta ctcgtatagt
     2521 tttctaaacg ttccacacct tcatccaata ccatcttaga ttgagatttc aagataagat
     2581 attggattac tgttgttttc acaccatcaa tatattctga tacaaatcgt tcttgctgat
     2641 ctattatgtc aaagactgct ttgttactcc ttgcatttcg tttttgtatt ttcatatttc
     2701 gagtttgtct ttccgtattc tgaggttgag atgaagtgat tttgatttca gctaaggaac
     2761 gatctctggt attttgatat tgtattgatt tcgattcctg agccataact tcttctggaa
     2821 aagcggttag tgatgtagcc ccatctagat tgaacattaa tgctacactc ccatctcgat
     2881 atacgattgt tccatcttgt ttcactttta aaatctctgt gcctcgaaat ggtttctttt
     2941 tccccataga tactcttgct gaattaacta ctgatttgaa cagtctaata gaaccagaaa
     3001 tcaatgaacc actagatgga tcaataaaca tatactggct aattagaata agaaatccaa
     3061 ttatcattaa taacgttgga acaaaaataa atttcagttt aatcgtatca gaaactaagt
     3121 aaacaattga tccaagaaca acgactgcaa caactatagc tgatacaatt aatctttttg
     3181 ctgtaacaga ataggtgcta ttgacatgct tttcaagcca ttcaactctg acaattgttt
     3241 cagaaggttt ttgatgtaag ttatgtttaa atccatagtt tgagttatct gccatcagaa
     3301 atacttcctt tcacgttcaa cttacggtaa acacctacag ttatcactat tgcaacaata
     3361 agcaagccaa ttaaagatat tgacttgctc attgtctcgt tcgtttgtgg tagttcattt
     3421 acctgataca cgagctgtgc ttcctgtaag tgctgagaag aggtcatgtt ttcttgattg
     3481 ttttgaatta ctggataaac tattttttct tctcgcataa tagccctcct tagttattat
     3541 cagataagtg agcttgaatt ttgtactgat ttccgtctgg agttttcaca actgcttttt
     3601 ctccatcaat agaaatcact tcagccttag ttgttttccc atcaatactg atgacaacat
     3661 ctttcccaac tttgaactct gtagttggtg ttgcttcata gctactattt gttgaagcag
     3721 agttcgtatt tgtcccaatc gaattttcca ctctcgaaat agatgtttga gtttgagcct
     3781 gtgcttgttg attattatga ctaataaagt aggacgatcc caagatcgtt ccgcaagcca
     3841 ttgcagcgac agaaatgata gtagccatct ttttacttgt aaaatcacgt tttgaacttg
     3901 attgattttc tgtagcaact gctaatgatt tcgttttttc tgccatttga gctagatcac
     3961 ttcgggaacg agataagtct tctcttaaag cttcactttg ttctgccaac ttttcgctat
     4021 ttaatttata accctctgct aaatctttga cctctttcat tcgatcttta tattcttcga
     4081 tagttttttg ttgctctaca agttttgttt ctaattcttt cgtgttgtta gctacctgct
     4141 tttcaacttc ttctttgttc gcaaaatatt tgacaaccac atctgttgtt ttctcttttg
     4201 tatcatggaa ctcagcttca ttttctgact ttgttttttc gacaatctct aaagtttttt
     4261 cttgcacgtc tgttaatgat ttttcaattt ctggtttcaa tgaattgata attcgttcca
     4321 ccacaacttc ttttgcttct tggctttgtt tcaatgcatc gtttttaata gattcaattg
     4381 cttcttggta ctgttgatca agactttctt cagttgcttt tctatattca tcttcttgtt
     4441 gtttaacttc tggtaaatgc tcagcatcat accgttgctc tagtttcttt agttcagcat
     4501 ccaaataagc ctgtttcgcc atatcataat ctttacgaat gatattggat ttttccatca
     4561 ctaaatcatc aattttagat gcttctgctt cgtagctctc tttagcaacc gatagtgtat
     4621 ttgtaaactg attttgacca tttaatgagg ttgcttcctc tatccgcaaa agctcatcag
     4681 ctaacatttg ctttcctttg aggatcaatt gctccttcgt cttatttaat tcactgttga
     4741 ttttattatt agcaatagag atgcgagcgt tcatactctc tttagctaaa aacagcaatg
     4801 gtttcaactc aggtatggat agcatctcct cgtttaaatc gacagggtca ttaatctcat
     4861 ctaaaactac aggttgaaat ttgtcagcaa tgatctgttc aaaatttttt tctaaaaaat
     4921 cagcatctaa gtttttcggt acatcacttt cagatgtgat tttttcagct tcataaacat
     4981 aattttcttt gtcactttgg gattcgggtt tttcatcaat ttttggttgt tggtaggttt
     5041 ctgtttgtat ttgtgtttct tctatgctta cttcttggtt ttctgcttcg gttggtttgt
     5101 tatcatccac aaaaagagct tctttttcat ctggtacatt ttctgctacg gcttcaaatt
     5161 caacttcttc agaagagttg aaggtctcag caattcgttc taatccatta gtagctactt
     5221 ctaattgctc tactgcgcta tagaagtctg tagcttcttc aacttctgat tcaaacacat
     5281 taggatcatc cggaataact aataattgac cactactatc cataaaacgg acacctgcta
     5341 atagcttctc tttaaatttt tctggtagat cagctacata attcaaactt gacttcaata
     5401 gaacgacata cttctcagaa tcgagaatag catcatcctc atctataact ccctcactgt
     5461 tgagcttttg tacatcttca attaccatat aatttttctt tggtaaatct ttggtcagca
     5521 acgcatcgaa ttctttccat acctttgctt tagctttttt tgccttcgct tctttttctt
     5581 cttttttgta cacttcgagt aattgtggct tcaaagagag tgcaactaca ggaactgttt
     5641 ctgccacata attaatagat tcggtaatat ctttgaattt tgccattatt gtaaaactcc
     5701 ttttttcagt caatagttct tttctgttta tctagcatat atacatatgc tttttcgaat
     5761 ttaataagcg tttcaagtct ggctttttca atctttttac tcccatttcg ataatcatag
     5821 aactggttaa cattcatatt cattatcttt gccagttcgg tacctttcag actgctcatt
     5881 aatacttcgt tggcgtgagc taacattttt tctctatcaa aaaccatctt acatttactt
     5941 tcttccctca cttctaaact acgcttggag cgtagttttt tggtaaaaag taaattgaac
     6001 aaatcggcat attatccaaa tttgttcaat tcacttttaa cctaacagtt cattttttgg
     6061 aaattttgaa actagataga taccaacaac tggaataagt gctgatatga tattagcttt
     6121 ttggttcaaa tcattacctg ttttagtacc cattgtgtaa agcataacgg ttgtaattac
     6181 aggaataata aagattgcaa taatcgcccc aacatatttt aatccatctc cccattttgc
     6241 atcaccaaaa aatttcattg cagtgaatcc tggtttcaac gaaaaaacga atgccaagaa
     6301 atacaaaaca acttgtacta aagttttcat atcaggcgtc attccgctaa ccaaattttt
     6361 aatagaatcg aacatataaa aacc
//
LOCUS       DAGCWU010000143         6216 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.144, whole genome shotgun sequence.
ACCESSION   DAGCWU010000143 DAGCWU010000000
VERSION     DAGCWU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.144"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            123..1094
                     /locus_tag="MBZ71_002298"
     CDS             123..1094
                     /locus_tag="MBZ71_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4430212.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            1126..2523
                     /locus_tag="MBZ71_002299"
     CDS             1126..2523
                     /locus_tag="MBZ71_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4430213.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            2682..3305
                     /locus_tag="MBZ71_002300"
     CDS             2682..3305
                     /locus_tag="MBZ71_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430214.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            4054..6147
                     /locus_tag="MBZ71_002301"
     CDS             4054..6147
                     /locus_tag="MBZ71_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBT4430215.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         2136 a         1043 c         1304 g         1733 t
ORIGIN      
        1 gctctactga agactttagt tatatggata ttgatgacaa gcaacactat gcaaagaatg
       61 cggtcatcat aatcgaacaa caagaaagag aaatcaaatg taactggcag gagtgagaat
      121 gtatggcatc gatccgtgat gtggctaaga tggctggtgt ttcagtggga acagtttccc
      181 gttacttgaa tggtcagcaa ctgaaggaaa agaacatgaa aaaaatagca gaagcgattt
      241 cagcgttgga ctataaagaa aacatcattg ctaaagggtt gaagaataat cgaagcttct
      301 ctattggact tttgataaac gggatttcca gccgttttgg gtcggaagtt gtctcaggga
      361 tcgaaagagt agctgaagaa aatgggtata gcttattatt aagcgggttt gccgatcagc
      421 cagaaaaaat tgaacaaaaa attgaatacc tgatgaagca tgtgatcgat gggctgattg
      481 tcttcttatc cgaagaagag tggtctggat ttgaaatgct cacaaaaatg agtattccag
      541 ttatcgcatt gaattgtcca gaaggaccag caggtattga tacaatatta gtgaatgatc
      601 gagaaagtgt agcaaaagtg atcgctcatc atggtgaaca aggacataaa aaaatcggat
      661 ttattgctgc cactcaaaca gactacgtag caagagaacg tttagcagga gccaaagaag
      721 cggtccaaaa tgatccatcg atccaattag aggtctttac aggagactat tctagaaaaa
      781 gcggctatta cggggcaaaa gcattattag aaaaaggggc aactgctatt tttgtcagca
      841 attacaatat gtcgattggt gccatcgaat gtttcaatca agaaggtgtc agaatcggga
      901 aggatattgc gttcagtcac tatgattatt tagataaaaa cgatctgagt atccttccga
      961 aaatcacgat cacgccacca acagataaaa tcggggaacg agcagcagaa cagctgttaa
     1021 aacgaatcaa aaaagaagag gaagaaaatg aaaaagtgat tgtgatggaa aatatcatac
     1081 atggaatcaa ctaaaacgaa ctaaaaaaga aaaagaggtg ctttgatgaa aaaatatcaa
     1141 tttccagcaa atttttggtg gggttctgca gcaagtggcc cacaaacaga aggaaggatt
     1201 tcaggcgatg gaaaaggaga gaacattttt gattattggt acaaaaaaga accgcaaaag
     1261 ttctttgatc aagtaggacc tgaaaaaacc tcgcaagttt atacgaagta tcaagaggat
     1321 gtacagttga tgaaacagac tgggcataat tcatttcgaa catctatcca atggagccgt
     1381 ttgatacctg atgaatctgg aacaatcaac gaagaagccg ttcgtttcta tcatcgttat
     1441 tttgatgagt tgattgaaaa cggtatcgaa ccctttgtga atctgtatca tttcgatatg
     1501 ccgatgtatc tgcaagaaaa aggcggatgg ttgaaccgag aaacggttga agcctatgaa
     1561 aaatatgcca aaagttgttt tgaattgttt ggttcgaaag taaaaaaatg gtttactcat
     1621 aatgaaccaa tcgttccagt cgaagcaggt tatttatatc gatggcatta tccagaagag
     1681 tcagatatga aaaaagcgat ccaagttggc tatcatgaag cgttggcttc agcgttagcc
     1741 atcaaatctt accatgaaat ggagcaaggt ggtgttattg gtatcatctt gaatctgaca
     1801 ccaagctatc caagagacga atcaaaccct gaagatgtta aagcagcgca aatagcagat
     1861 gcgttcttca atcgttcatt ccttgatcca gctgtaaaag gcgagtttcc aagtgaattg
     1921 attgagatag taaaagaact tgatctcatg cctgtgatcc atgcagagga tttgacggtt
     1981 atcaaagaaa atacagtaga tcttttaggt atcaattatt accagccacg ccgggtaaaa
     2041 gcaaaagaaa caccaatcga tactaaaaac ggtccaatgc ctgaggatta tttcgacaat
     2101 tatgaaatgc ctggtcgtaa aatgaatcct tatcgcggat gggagatcta tgaacaagga
     2161 atctatgata ttttgatcaa tgtacgagat cattatggaa atatcgactg ctttatttct
     2221 gaaaacggta tgggtgtaga aaacgaagca cgatttatca aagaagatgg catgatccac
     2281 gatgattatc ggatcgaatt tgtccaatct catctgaaat atgtccatca agccattcaa
     2341 gaaggtgcca attgtttagg ctatcatatg tggacatgta tggataactg gtcgtggacc
     2401 aatgcatata agaatcgata tggctttatt tcagtagact tagcacaaga tggaaaaaga
     2461 acaatcaaaa aatcgggtca ttggttcaaa gacgtatcag ataaaaacgg atttgaggct
     2521 taagaaagaa tagttgagag caaatgcgtg aaattctcca ttcttaccat tttgtgaaaa
     2581 agaagttcaa ttatttacga tctttttcaa cttttttgtc tttctatttc attttttgta
     2641 tataatagaa tagtcaaaat taaataggag tggaggaaag catgaggaaa aaaagaccgt
     2701 ttcttctctt ttggttattc ggttttttct tactgcttat tttatccgca gcattttcag
     2761 gaaaaatgac agcagatctt gctcagaaag aaacagaaaa aatcgtagca agcaaagaat
     2821 cagtagataa tttcaaaaaa gaattctcaa cttattggtg ggcggagtat aaaataactg
     2881 ccaattacga aaaaggcgaa ttggacattt atttgccaaa aggaaaagaa gaaaatcaat
     2941 ttattaatat cgatgacgta cttagtgcga tttctctcag acaatatcaa tttaaagaca
     3001 agcatttgac cgttttgctt ttgactcatg atggcgacaa agtagcccgt ttgaaaaatg
     3061 gaaaagtgat caccgcttat tctgaaaaag tagacaaaaa agtggtcgcc gatcgtttag
     3121 cggaatggaa aaagacaaat gaacaagtca agaaaacaac tgtctcttct caaaaaaacg
     3181 agtcgacagg aacaagtcct tcagattctg gcagtggaca aacgataacg ggaagtgaag
     3241 aaaatcaagc ctcaattatt acgaatgagt catcacaagg acaatatcaa tcctatggac
     3301 aatgaaaaac agaggttgtg aaatcagcgt tttgacctga gtattagaga aacaaacggc
     3361 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac
     3421 accgtgtatc aagaggtctt gagcctgccg tttaactctg agccacaagg agcaatttca
     3481 aaaaatagct ccttatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg
     3541 aacaccgttt accaagaggt tgtaaaatta gcgtcctgac ataatctaat accgagggtc
     3601 agctgattga acaccgtgta tcaagaggtc ttgagcctgc cgtttaactc tgagccacaa
     3661 ggaacaattt caaaaaacag cttctcatgt tttataaata ttgtgacttg tggccgagga
     3721 gttggcagga gaacaccgtt taaggaagaa gttgtgaaaa agctgtcctg catcaagaaa
     3781 taaggagctg cgacaagaat tttgtcacag cctctttttt tgtacgaata ccgagaaact
     3841 ggcttttgaa taccgtggag tgaagcggtc accacaagac ttttatctct tcattagttt
     3901 aatcgcttac atcgcagcgg ttttatgctg tgaaaagtgg acggaataat ctttagaaag
     3961 aataaaaaaa tcttaagata acaacttttt taggaaattt tcatttttca ggagcaaaat
     4021 aagaaaaaat gtgaatagag agaagtgata tacgtgacac aacttaaaaa attgtgccaa
     4081 aatcgtttag caatcgtttt gacagctgtt ttctttttct ggctgaagac gatcattgcc
     4141 tactatgcag atttttcatt aggtgtggaa ggtacgatcc agtactttat cttgtggatc
     4201 aacccaattg cgactacctt attgtttttt ggcctttctt tgtatattaa aaagccaaag
     4261 ccagcgttag ctattctttt gatcattgat attttaaata cgcttttgtt gtacttgaat
     4321 attatttttt atcgggaatt cacagatttc attacagtca aatctgtatt aggcttttcg
     4381 aaagtgtcgc aaggattgtc aggaagctcc ttttctttga tgaagcccca tgatgtcatc
     4441 tactggctgg atatcgctgt gttcattggg ctactggttt ggttgaaggt caagaaaata
     4501 cctatcaaaa gcaaccctgt cggcaaaccg atggctatcg ctgttacctg tctttcaggg
     4561 cttatttttt caggaaatct agcgctgagc gaagccaacc gtccacaatt gctgcagcga
     4621 acatttgacc gttcatatat tgtcaaatat ctaggaatcg atgcgtatac tatttatgac
     4681 ggaatcaaaa caggaatgac tagcagtgtt cgcgcgcatg caagcagtaa tgggattgat
     4741 gaagttttag attatacaaa gaaacattat gcagagccaa atcccgaaac atttgggatc
     4801 gctaaaggga aaaatgtgat cgttctccat ttagaaagtt tccagcagtt tttgattaat
     4861 atgaaggtag acggacaaga agtcacaccg tttttaaata gtattttcca gaatcaagca
     4921 acgatcagct ttgacaattt ctttcatgaa gtaggacagg gaaagacaag tgatgctgaa
     4981 aatatgttgg aaactggcac atttggttta cctcaaggct cattattcac agaacttggg
     5041 tcagacaatg tctttcaagc cgcaccagcg atccttggac aaaagcaagg ctatacaagc
     5101 gctgttttcc atgggaatgt agctagtttt tggaatagag atcatgtata taaaaactta
     5161 ggttatgaca atttctttga tcgatcttat tttgatgaat ccgacgaaac gttaggttat
     5221 gggatattag ataaagattt attcagagaa tctgcccaat atcttgagca tctacaacag
     5281 ccgttttaca cgaaatttct ttccgtaacc aaccacacgc cttattatac ggatgacaag
     5341 aatttcgact ttccatcttt gaatactggc aatagcacag tagataatta tgttcgaacg
     5401 gcacattatc tggatcaatc attggaacaa ttttttacgt atttgaaaaa atctggtatt
     5461 taccaaaatt caatctttgt gatttacggg gatcattttg ggatctcgaa tacagataat
     5521 aaagatttag caagcgctct tggtaaagat ccagatacat gggatgaatt tgacaacgca
     5581 cagatgcaac gagtgccatt gatgatccat atgcctggtt atactaaagg gacagtcaat
     5641 catgaatacg gtggagaaat cgatgttttg ccgacattac tacatctatt gggaatcgat
     5701 gataaagatt atcttcattt tggtacagac ttattatctt ctcagcatga tcaagtagtt
     5761 gcttttcgaa atgggaactt tgtgacaccc aaatacaccg tcctaggagg aaaagcttac
     5821 aataatcaga caggagaaat catcgatacg aaagcagcag gaatcgatga ggaaatctcc
     5881 aaagatcagg aaaaagtcaa gactgcgtta agtctttcag ataagctgaa tcaggaaaat
     5941 ctcttgcggt tttatgtgcc aaacggattt gagacgattg atccgaaaaa atatgactat
     6001 aaaaatgaag cggaacgtaa tgaagcaatc gaaaagcaaa aaggcgaaca ttcgacaagt
     6061 gtcttttcaa aaaatggtaa taaaacaaca accaacttat atccagtaac aagaacagac
     6121 gttgaacagc aaaaaaataa tcaataaaca atcaaaccaa ccttcagtag ttagacaaca
     6181 ttctaatgct tgaaggttgg tattttttat gaaaaa
//
LOCUS       DAGCWU010000144         6173 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.145, whole genome shotgun sequence.
ACCESSION   DAGCWU010000144 DAGCWU010000000
VERSION     DAGCWU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.145"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            725..811
                     /locus_tag="MBZ71_002302"
     CDS             725..811
                     /locus_tag="MBZ71_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBT4430216.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="MBZ71_002303"
     CDS             complement(932..1621)
                     /locus_tag="MBZ71_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430217.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="MBZ71_002304"
     CDS             complement(1866..2780)
                     /locus_tag="MBZ71_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4430218.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="MBZ71_002305"
     CDS             complement(2762..3142)
                     /locus_tag="MBZ71_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBT4430219.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="MBZ71_002306"
     CDS             complement(3592..3879)
                     /locus_tag="MBZ71_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBT4430220.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="MBZ71_002307"
     CDS             complement(3899..4123)
                     /locus_tag="MBZ71_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBT4430221.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="MBZ71_002308"
     CDS             complement(4578..4748)
                     /locus_tag="MBZ71_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430222.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="MBZ71_002309"
     CDS             complement(4763..5296)
                     /locus_tag="MBZ71_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBT4430223.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="MBZ71_002310"
     CDS             complement(5317..6159)
                     /locus_tag="MBZ71_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4430224.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAGCWU010000145         6147 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.146, whole genome shotgun sequence.
ACCESSION   DAGCWU010000145 DAGCWU010000000
VERSION     DAGCWU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.146"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            508..1782
                     /locus_tag="MBZ71_002311"
     CDS             508..1782
                     /locus_tag="MBZ71_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4430225.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1836..3263
                     /locus_tag="MBZ71_002312"
     CDS             1836..3263
                     /locus_tag="MBZ71_002312"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBT4430226.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            3276..4148
                     /locus_tag="MBZ71_002313"
     CDS             3276..4148
                     /locus_tag="MBZ71_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4430227.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            4243..5238
                     /locus_tag="MBZ71_002314"
     CDS             4243..5238
                     /locus_tag="MBZ71_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4430228.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(5843..5950)
                     /locus_tag="MBZ71_002315"
                     /pseudo
     CDS             complement(5843..5950)
                     /locus_tag="MBZ71_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2140 a          838 c         1164 g         2005 t
ORIGIN      
        1 tcgttattga gaagatctag aaggccagcc aactttatca aaaatcatca ttcctgtcac
       61 tcgtatcgtt ttagaagctg ctcgagtaag taatggagaa actgttaaat aattttaagt
      121 aacagttaag tcgttagttg ggttatcaat ggctattccg tccttatctg aacctcgaaa
      181 cccaatcata gaaaaagttt tttgaacaag ttttaacaga taaatcaagt tatatactag
      241 aaaattcgta aaatatattt ttaatgtcat aatcgtttga tttttctgga ttgacattta
      301 tttataaaaa atagtgccat taaaaaagaa aagtgacaca ccaataaaag tcatatcctg
      361 tgacattata tggcattatc caaatagaat aaccggttga catgtaagcg gattcctttt
      421 ataattttag gagtataagt ccttttctaa aaatattaac cggttatcat gaaaggggat
      481 acaaatatat cgttggagga taaaaatatg tcaaaggtta acgtatttaa aaatcaatcc
      541 tatttacaaa gttcagctac attattacta ttttttgctt cttggggtgt ttggtggtca
      601 ttttttcaac tttggctaac atctgaatca aatggtttag ggttatctgg cagtgctgta
      661 ggaacagtat tctcggcaaa ttcgttagtt accttaattt tgatgtttat ttatggaaca
      721 ttacaagata aattgtatat taaacgaaat ttattaattt ttgcttctgt attagcgaca
      781 cttgttggac cattttttat atggatatac gggccattgc tagataacaa ttttaattta
      841 ggcattatta tgggagcact atttttgtca gctggatatt tagcttctgt aggagttttt
      901 gaagctgtgt cagaaaggtt tagtcgttta tttggctttg aatatggaca agcaagggcg
      961 tggggatcat ttggttatgc cttggtagcg cttttggcag gatttttatt tgtaaaaaat
     1021 cctcatttaa acttttgggc gggatctttc tttggttctt tactattgtt aaatttatta
     1081 ttttggaacc ctaaagttga acgggaagca aatcaaaatt ttaatcaaga acaagctgaa
     1141 tcaaatagta ttccttcttt aaaagaaatg tttgatctaa tgaaactgcc tcaattatgg
     1201 acgataatca tctttattgt ttttacatgg acattttata cggtattcga tcaacaaatg
     1261 tttccgggat tttatactgg tttgttttca acatcagcta atggtgaaaa aatatatggg
     1321 acattgaatg ctattcaagt attttgtgaa gcgttaatga tgggaattgt tccaatcatt
     1381 atgagaaaat taggggttcg aaatactttg ttattaggtg taaccattat gtgtgtacga
     1441 attggattgt gcgggtttgc ctcgacacca ttatctgttt catgcataaa aatgttgcat
     1501 gctttagaag taccattatt tacattacca atgtttcgct attttacact tcattttgat
     1561 acaaagctat cagcaaccct ctatatgata ggatttcaga tagctgctca aattgggcaa
     1621 gtgattttat caacaccatt gggaatatta agagacaacg ttggctatca accaacattt
     1681 aaaattattt ctcttattgt attactagca ggcatatatg cattctttat tcttaaacaa
     1741 gatgatagag atgttcaagg ggatccattt attcgaggat aatggaaacc aacagaataa
     1801 aaaacaattt tatctagaaa gtgagcgaat atattatgac aaaaagagct atagaattaa
     1861 caaatacacg ttatcgtttg ggatatcatg tgtctgcacc atcaggatgg attaatgatc
     1921 caaatggatt ttgctatttt gatgggtatt atcatgtgtt ttatcaacat catccatata
     1981 gtgctgaatg gggaccaatg cactgggcac atgctagaag taaggattta gttcattggg
     2041 aatctttacc acttgctttg actcctggag atcaagaaga tgaaggtggt tgtttttcag
     2101 gaagcgctat tgaaaaaaat ggtgtgttgt atctgttcta tacaggacat cactattatg
     2161 gagacaatga tccagatcat ttttggcaaa atcaaaacat ggcgtatagt acggatggca
     2221 ttcactttac taaatatgaa aaaaatcctg taatagcaaa agcaccagaa gataacacac
     2281 atcatttcag agatccaaaa gtatggaaac acgatgaaaa atattacatg gttttgggta
     2341 gtcaagaaaa agatggatta ggtcgtgcta ttgtttatca gtcgttagat ttattaaatt
     2401 gggattacat tggaccaatt tctaaagcga aggaagtact tacagaagga tttatgtggg
     2461 aatgcccaga cttttttgaa ttaaatggaa aatctattct tttattatcc cctcagggaa
     2521 ttgaagcaaa tggaaaagat tatttaaatc tatatgaaac aggctacttt atcggtgatt
     2581 atgattatga gacagcaaaa ttcattagag gaaattttta tgaattagat aagggacacg
     2641 atttctatgc aacacaaaca actttatctc cagatggtag acgtattgtt atggcttgga
     2701 tggatatgtg ggaaagccct atgcctgaaa aagtagacgg atgggcagga gcattaacgc
     2761 ttcctcgaga attagagtta attggagatc atttgtatat gaaacctgtg agagaattag
     2821 aacaacttcg agttggcagc ggcgttgaaa caaaagttaa tttggcagga gaaataataa
     2881 tcgctcaaga tgcttcattc aaagaagctc taattgatgt actattgact aaatcaaaaa
     2941 atcaagaaat caaattttca ttaaaaacaa aagatgaaga gttaattaat attttttatt
     3001 ctaaggataa aaatgaatgt accttaactc gtaacggcaa aaatgatcca aggtatggaa
     3061 ccattcagat gtgtgataga ttatcacttc gagtgtttat cgataaaagt tcggcagaga
     3121 tttttattaa tgatggcgag cttgttttta cagagcgtta ttatacagaa aatcaaccaa
     3181 gtatgcattt gagtgttagt gaagaagctt ttgtctcgag tacaatttat catctaaaaa
     3241 aagatgcaat ttcttacgag taaggaggtt ttatgatgtt aattggtagc atagaagcag
     3301 gtgggactaa atttgtatgt gccgtgggag acgaggatta ccgagtacaa gaccaaataa
     3361 tttgtccaac gaccaccccc agggaaacat tgaaaaaaac tgttgactat tttaaacagt
     3421 ttgatataaa tgcattaggt attgcatcat ttggtccaat agaaattcga aaaaattcac
     3481 caaaatcagg atatattacg tctacaccaa aggtgggatg gcgaaatgtt aatgtgttag
     3541 gttatttaca agatgagctg aatgttccaa tgtcttggac aacggatgtg aacggatcag
     3601 cttatggaga gtacatcatg tccactttat cgaatgaaaa aataaattca ttagtttatt
     3661 acaccgttgg tactggcgtt ggtggtggag ctgttgttga cggaaaacta ttaggatcgt
     3721 ttggtcattc agaaatgggc catacgtttg taaaacgcca tcctgatgat attgatttta
     3781 aaggaatttg tccttatcat ggtgattgtt tagaaggctt agttgcggga ccaacgtttg
     3841 aagcacgttt tggaaaaaag ggtgaagacg tttctttaac ggatcctatt tgggatgtaa
     3901 tggcctatta tctagcacaa gctgtgattc aaacaacatt gattatacga ccggataaaa
     3961 tagtatttgg tggaagcgta gtaaatgaaa aattgttaga aaaaattcgt gttaatgtta
     4021 atgaattatt ggatgattac gtggaaatac caccgttaag taaatatatt acgcgtcctt
     4081 taattaaaaa taatggatcg gcaacactag gaaactttgc tttgggcttg aatcaattac
     4141 aagaataaaa catagaaaaa atatcgatta tcaggtatag tagttctata gagttctttt
     4201 tttcgatagt taattattgc attatttagg agagacaaga gaatgcgccc aaaattagaa
     4261 gatgttgcca aaagagcaaa tgtatcaaaa acaactgttt cacgtgtatt aaacaatcgt
     4321 ggctatttaa gtcaaaaaac gattgataat gtttataagg ctatagaaga actcaattat
     4381 caaccgaacg ttgtagcgcg tcaattattt caaaagaaaa ctaacattgt cgggttacta
     4441 tttcctacag tagccaatcc tttttttagt gaactcgttg aggcattgga aaaaaagtta
     4501 tatgaaattg gttataaggt attaataggc aattcgatga ataataaaga aaaggaaaca
     4561 aattatttaa atcagttatt gtctgatcaa gtagatggtt taattgttgg tacacataac
     4621 caagggattc aggaatataa atatcagaat ttaccgattg ttgcaattga tcgagtgatg
     4681 aatgaagata ttccagttgt tgaatccgat aactacaatg gtggaaaact tgcaaccaag
     4741 ttattaattg cacaaggtgc aaaaaatatt attcatacaa acggtccaat tgacttacag
     4801 actccggcta atcgtcggag actagcctat gaagatacta tgaaagcata tcagttaatt
     4861 ccacgaactg tgacattaga tttcaatatt tcttatgtaa agaaaaaaca gatttttttc
     4921 caaatgtttg aggattatcc taaaatagat ggtatttttg cttcaaatga catcgatgcg
     4981 gccctaatat tacaagtagc aaaagaaaag gggttaaatg taccagctga tttattagta
     5041 gtcggatatg atggtacttt aatgactcgg agtatattac cagatctgac aacagtaata
     5101 caaccaatta atgatatagc agacacagct gttgctattt tgatgaaacg aataaataaa
     5161 gaagaaacaa aaaaggaata tattttacct gtcacattat ggatggggaa gacatcagta
     5221 aagaaaaagg acaattaaat ttataaagac tatcaatgat aatttcatta ttaattcgtg
     5281 atataaaaag ttgttaaatc tcttataata gactatgata caaaatcatg cttttaatct
     5341 gtgtagatgt tgccaaatat ttgaaagaca tagaacttgt cagaaaaacc ttaacaattc
     5401 ctcaatgggc ggataagcta ggcaaaaggg ctggcataaa cttttcagtc cttctaacag
     5461 aatcaattgc agataaagca gataacattc ttctttctgg aagaaataat tagaactcaa
     5521 aagggtcaga aacttaaaag tttttgatcc tttattttca agtctgattt gacagatttt
     5581 cggcagctca tttatttatg gtcagaaata gttgctgaaa ttttaatcta ttagcaaata
     5641 gtccagaacg tatgaagagg attcggatat catttgtcat taacaccata aatatttata
     5701 aaattcgtgt tcacctacaa ttaaatttaa tatataatat tcgttgtaag ggcttataat
     5761 tttttgttaa tgaacaagat atgtaattga tagtgtcaat agctattcaa aaatgtaggc
     5821 ttttggccca taaataacaa ggttacttat atgataactt tttttcttta taatctttaa
     5881 tccgatagag ctttccagta gtttgaataa tcttagcgtg gtgcactaat cgattgatta
     5941 gtgcatttgt gttcagtcca ttgagacata atagtggata gttttactga gaaaattgat
     6001 aaaaaataga gatataattc aaatacgtat ataatgaaac taaagataaa tacggaggaa
     6061 aatatatgat tataaaaaac ttaggaaatg ttaaatcaat agatatacac gttaaagatt
     6121 tgctgatttt tactgggaaa aataata
//
LOCUS       DAGCWU010000146         6128 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.147, whole genome shotgun sequence.
ACCESSION   DAGCWU010000146 DAGCWU010000000
VERSION     DAGCWU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.147"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..476)
                     /locus_tag="MBZ71_002316"
     CDS             complement(<1..476)
                     /locus_tag="MBZ71_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBT4430229.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKSLETWFHTVDRRMIGTIGTLILFTYY"
     gene            complement(617..1219)
                     /locus_tag="MBZ71_002317"
     CDS             complement(617..1219)
                     /locus_tag="MBZ71_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBT4430230.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            1465..1671
                     /locus_tag="MBZ71_002318"
     CDS             1465..1671
                     /locus_tag="MBZ71_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430231.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            1984..2703
                     /locus_tag="MBZ71_002319"
     CDS             1984..2703
                     /locus_tag="MBZ71_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBT4430232.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(2784..3467)
                     /locus_tag="MBZ71_002320"
     CDS             complement(2784..3467)
                     /locus_tag="MBZ71_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBT4430233.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            3699..5885
                     /locus_tag="MBZ71_002321"
     CDS             3699..5885
                     /locus_tag="MBZ71_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBT4430234.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2306 a         1024 c         1156 g         1642 t
ORIGIN      
        1 atatagtatg taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg
       61 tgaaaccaag tctctaacga ttttttaaaa caaaaagcta ataaagcaga taaaaaagcg
      121 gataaggcaa tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa
      181 acaaaaaaat cgccgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa
      241 ggaagcgcgt aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc
      301 attcctatag caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa
      361 atacaagaaa tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc
      421 aataagatac aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag
      481 tacagctccc attgattttt accaaattat agcacaatta gcgtattatt tttttaattt
      541 ttcagggatt ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga
      601 taagttagtt aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca
      661 agtagtttgt aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg
      721 tagaaaagta agaagctgag aaaaataaag taatgagaga aaaaattaga aacatgctga
      781 tcgccttctt taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc
      841 ttgatccata gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga
      901 aggagatagt caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg
      961 tccacagcag acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc
     1021 atatataaga aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga
     1081 ctagaacgac cgctagtttt ccaagattat tcaataaaac ttctaaggca aattttactt
     1141 ggacatacac catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt
     1201 tcacaacttt atctttcatc tcatacgtac ctctaccaca aattttatgc taaaaaaaat
     1261 aaaaaacgtc ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatttccta
     1321 aaaaaatctt cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt
     1381 actttttatg acaagtttgt gctttttcga gtgatttatg ttaaaaaaat tactgacctt
     1441 ataaaaggtg atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca
     1501 tatgcattta aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat
     1561 gaaaatagtg tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat
     1621 aatctgcact ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata
     1681 aaatgttgag agcataaaga ggttgtgagt ctccgtttag ctctgagtta caaggaataa
     1741 tgttaaaaaa cagcttttca tgttttatga atattgccac ttgtgaccga ggtgttggca
     1801 ggagaatact gtttatttga aaaaagggct gcgacaagac ttttgtcaca gcctttattt
     1861 tcatgaatag gagttacttt tggctacttt tagagaagta gcttttaagg agcagataaa
     1921 gtcaaggctt ttatttctat aaatgaagta gtaaaatgaa gaacagattt tattacagga
     1981 aggatgccga taattgtgat cattgttgca ggaacgatcg gtgcagggaa aagcacactg
     2041 actgaaatgc ttgcccaaga tttagaaaca aaaccttttt atgagaacgt ggaagataat
     2101 gaagtattgc cgctatttta ttctaatcca gaaaagtata cttttttatt acagattttc
     2161 tttttgaaca aacgcttttt ggcaatcaaa gatgctttca gccatgatga caatgtacta
     2221 gaccgctcca tctacgaaga tagtatgctg tttcatttga atgcagattt agggcgtgtt
     2281 tctgaagtag aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa
     2341 gaaatttcgc cacaaaagaa accagatcta ctggtttata ttcgtgtatc ttttgaaact
     2401 atgttagcga gaatcaaaaa aagaggccgg gaatatgaac agctagaaca agatcctgaa
     2461 ctctattcct actataaaga actcaatcga cgatacgaag agtggtatga acagtttgat
     2521 atatgtccca aaatagtgat cgatggggac aaatatgatt tcgtagccga tccggcatgt
     2581 ggagaacaaa tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga agaggcagat
     2641 gcccagtcga tccgctacac tacaaataac ccgaaagcaa agggaatctt tacaactaaa
     2701 taatataaaa actaagcaaa ttgctcactc gcagatattt ctctgtgagc agcatcttgc
     2761 ttagtttttt tgattcattg agatcaaata gtgaaccgat tcgtatgata aaattgccat
     2821 aaatgaaatg tctgagtgag ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa
     2881 tgggcgccaa acagatgatg acagaaaaca aggacaatag ctcctgcgat aaaggcaaga
     2941 acgacactta gatagtggaa aaatcgatca aaatctgctt tttctttaga aatacaataa
     3001 tcatataagt tcgttgctac cccttttaaa tttccagttg taaataaatt agtgtaggca
     3061 cgcccgttga ttttatcaaa agctacccac tggatcgatg cagtaaaagc aatcaaaatc
     3121 gttactaatg cgggatgctc atggacgggt gtcaaactaa caaagaaaaa aatcactgct
     3181 tcataatata gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa ataatcaatt
     3241 agatactttg ttagaagaat ccctaagaaa aagaaaaggg tagacaatac ttttttacca
     3301 acacttagcc attctccgtc aaatgcttgg atgatcgata gaatgatatt gccagtttga
     3361 gcagaggcaa aagcaccata ttggatataa gtataactgt ccatggctcc tccgataaat
     3421 gtcagcaaaa gtcctattgt acgcccctcg tgaaaggaag tctgcatttt tttcatcacc
     3481 ttttatgaat atttttgtct tccttatctt agcataaacg attgaaaaaa aggaaaaatt
     3541 ttcagaaaac taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata
     3601 aagaaaaaat agacaacgaa actttaaata accaacttga aataaccaac ttgaaataaa
     3661 caaattcaaa gaagaaagat gggaaggaaa tcaatcagtt gaatgaaaaa gaaattctcc
     3721 gattacaaaa agaattgaaa cgctgggaga acaagtataa aaagcttcaa caagtctttc
     3781 ggctacaaga agaaatcatt gctacctatg aagcggctta tggcgaagca cccggactta
     3841 ttgaggaaga agtacaagta aaagaaagtg aaggaaacag ctctttcttt aaaatgatgg
     3901 agaaacgttc ctataatcgc ttcaatcaag aacaaaaagc agctatcaca tatgatatga
     3961 agaaacatct tcgaatcata gcaggagctg ggagcggcaa aacgcaaaca atctgtgcaa
     4021 aagctgttta tctgatgacg caaaaacaag tagacgaaga acgcatacta atgatcactt
     4081 ttacaaggaa cgctgcaaat gaactaaaaa aacgagtaga taatttcagc cagagaaaaa
     4141 ccgctgtcca tatcggaaca ttccatagta tcttttttag gatatacaat gaaatttgtc
     4201 gaaaatttcc tgaagtagca atgcaaggaa tacaaggaga cttttcaaag gatactgcac
     4261 aaaaagtgaa tgctgttttg caacagctga tacgtaaata taatctttat atatttgatc
     4321 aatatggaga aaaaacgatt gcttctcggt tagactattg gcagaatatg aacctttcag
     4381 tagaagaaat ggttcagtta gttaaagaaa aatatgattc cattgataag aatgcacgac
     4441 agccgatatc agaacgactg tatggattgc tcacggaact ccaagaacaa aaaagaagcc
     4501 agcaattgct agagttcaat gatgtactcc aaaatctgaa aagcgctttg gagaatgagg
     4561 agatccagca ttatatcggc caaaaatatg attatctatt cattgatgaa tttcaagata
     4621 ctaatccact tcaatgggaa atcgtgaaac ggatgacaaa ggaaaacagg atcaagttga
     4681 ttgttgttgg agatgatgat cagagtatct atggattcag aggatcagag ccagcataca
     4741 tcaagaactt tgaaaaagaa tatcctacca aaactctttt cttattaact aattatcgtt
     4801 ctcgggcagc tattgtccaa ggagcaaata gattgatcag ctataataaa aatgatcgga
     4861 taccaaaggc gatgattcca gcacaaaaag aagcaggtgt tatggaagcc tatttgtttt
     4921 ctgatactaa agcagaaagt cagtggctaa tcaaacaaat tcagacattt attcaaaaaa
     4981 atgggaaata taaggaatca atcatattat accgatcagc ttcacaaacg cagcagcttg
     5041 tccaatctct gctgaaaacg aatattcctt ttgtactgga agcagatagt ccatatgaag
     5101 gtatctttgg tatcaagtct ttccaacatt tctatcaaaa aattgttcat tggcaaacag
     5161 cactgaatct gcaaaagaaa aatcaggcct accagcaatt gctccggcag ctgatggcag
     5221 actgctacct gaaaaaaagt gaatgcgatc aatacttttc tcctaaatat caaaatatag
     5281 ccgttgcaga ttatattttg tcgataagcc ccaatttaaa atcaaaatct gcagaaatcc
     5341 gagagtttga ggaagcgttg cgtaaaacag accagcatca agaaatctat tcgcttgttc
     5401 aaagctatct tcatctgcca cgtatagcta aggaaatcga ccagagtgat caagaatgga
     5461 tacgagaaga agtaaagcag catgctactt tcagaagact ccaagcctta tcgcaagaaa
     5521 cccaagcgac agctaaagaa ttaaaaaccc gcttgattgc ttacagacaa ggaaaacttg
     5581 atgcactatg tatccagagc attcacaaaa gtaaaggtct tagttaccga aatgtctttt
     5641 tgattggttg taacgaagga agtttgcctt ataacggagc gacagaaaag aaagtaatcg
     5701 actttaatga aataaaagca gaaccagtga cgacgataga agaagaacga agactatttt
     5761 atgttgcgat gactagagca agaaatcgtc tgtatctatg tgtacctcaa atgaaaaatt
     5821 caagaaaatt gaaagtatcg agatttatca aagaaaccgg gagtcgtgtg aaaaaaggaa
     5881 agtgatagaa gagagggtgt gacagaagtg gtcagtttca agaaagaaga aggaatgtac
     5941 gaaaattgct tctcaaattt ttgtgaaatt tcagcttatt ttcgaagaag ctatttctgg
     6001 aacaccgttt attcggaaaa aggaggtgtg acaagaattt tgtcacacct cccttattta
     6061 ttttgaaaat gagaatacta ttatcaaaat agagaatata tagaataatt ctcattattc
     6121 aaacgtaa
//
LOCUS       DAGCWU010000147         6124 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.148, whole genome shotgun sequence.
ACCESSION   DAGCWU010000147 DAGCWU010000000
VERSION     DAGCWU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.148"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(168..470)
                     /locus_tag="MBZ71_002322"
     CDS             complement(168..470)
                     /locus_tag="MBZ71_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430235.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(690..1056)
                     /gene="ssrA"
                     /locus_tag="MBZ71_002323"
     tmRNA           complement(690..1056)
                     /gene="ssrA"
                     /locus_tag="MBZ71_002323"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1227..2000)
                     /locus_tag="MBZ71_002324"
     CDS             complement(1227..2000)
                     /locus_tag="MBZ71_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4430236.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2144..2494
                     /locus_tag="MBZ71_002325"
     CDS             2144..2494
                     /locus_tag="MBZ71_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBT4430237.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2475..3191
                     /locus_tag="MBZ71_002326"
     CDS             2475..3191
                     /locus_tag="MBZ71_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4430238.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3191..4639
                     /locus_tag="MBZ71_002327"
     CDS             3191..4639
                     /locus_tag="MBZ71_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4430239.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4709..5218)
                     /locus_tag="MBZ71_002328"
     CDS             complement(4709..5218)
                     /locus_tag="MBZ71_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430240.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5208..5933)
                     /locus_tag="MBZ71_002329"
     CDS             complement(5208..5933)
                     /locus_tag="MBZ71_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430241.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2089 a         1061 c         1182 g         1792 t
ORIGIN      
        1 aattggagtg gtagttaatt agtgacttat ttttgatttt atagcactga tactataaaa
       61 tatagatatc atcatattac acaatcttaa tactaactta aaaaatatcc cttttataag
      121 tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta atctttatac
      181 ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc gctccgtgcg
      241 tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat tctggttttt
      301 gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa taagctatct
      361 tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta
      421 ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat aatgttcacc
      481 tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat tgataatatg
      541 gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg ccattttcat
      601 tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaaa taaggattta
      661 agcatctagc gaaatctgct gaaatctcac aatggagacg gcgggagtcg aacccgcgtc
      721 caaacacatc gccacttaaa aatctacgtt catagtcaac tcatttaagg attcacttta
      781 cagcttgccg agtgacaggc attctgtatc gctagtctga tgttctcttt ttcaacttac
      841 agacggaaag ttgaaacgta tcccactaaa gttaggaccc ttactcgagc acatgggcga
      901 tgccgagagg atctacgcta actgttttta ggcagctaaa gcgtaagagt tgttttcgtt
      961 tttagcagtt atatttaact gtaacgtttt tacgtagacg taacctacga aacgcaattc
     1021 aagctcgact gtgcctgtcg aatccgtaac gtccccgtta gctaacaagg gttacgtgta
     1081 tttttcatac aaaattagta tagcatatat tgggataaat tcaataaaaa agcgaatcat
     1141 atcccattat ttatataata actgcccctt tcttaagaaa aaataagcga ttttcttaag
     1201 aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa aatacttcaa tgcgtggaca
     1261 attccgcctt ctgtattttt ctttgtgata aaggtagaaa gttcttttaa tccgtcatag
     1321 gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca aggcaaaatc attcggacca
     1381 tcgccaaatc caaaagtagg aactttttcc agctgaagtt ctttttgcag acgtttgaca
     1441 cctgcccctt ttgagattcc tttcttcact gtatcgatcg agtaaggacc attccgataa
     1501 aaagttagct caggaaaatg ttcgtgatag tattcgtcac ctgattgaga taagatcagc
     1561 aacatattta ctgtgttttc tttaaagcct aaaggatcaa tttccggaac atctgaatga
     1621 atgaagtcgt aagcattgat cattgtttgg ctatgaccgg tacaccagat tttctgatcg
     1681 ttatagtaag aaagttcatg accgttcaac tgtacatgat catacatttt ttgacattct
     1741 tctgaagaaa atgtttgaga atagatttct tcgccgtcta ctcgaatgaa agaaccattc
     1801 atggtgaccg cagaagtaat gcccgcatct tccatgatag agtggatttc tgcttctgta
     1861 cgtcctgtag cgatgacggg gagaacattg ttttctttta atgcgttcat agcttttgcc
     1921 acttcgggtg tgatttttga tttcgcatct agtaatgttc catcaagatc aaagaaagtg
     1981 attgcacgat aatttgacac aaataaaact ccttttttat tggaataagt ttatcttaac
     2041 aaagaatgaa caaaagacaa acctttcgtg aagaattcct gaacatattg gttcagtcaa
     2101 caatttagag tatactataa gaagagaatt taggagggaa agaatgattt ttttcagtat
     2161 tggtatctta ttcttaataa ttggcagtct ttttcggatt ctgccctcaa aaggaaatct
     2221 gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca gacgctcatt ggcggctggc
     2281 tcaaaagaca agcggaaact ggttttttct tatgggctta ctgatggcat tgatcggcta
     2341 ttatttaaag acatctgggc atacgaatta ttttctgatt gaaatgcttc tgctagtatt
     2401 tccaattatg cctattttta ttatgacaga aaagaaatta cagaaatatg acctggaaac
     2461 tggagggaac gataatgaat attttaatga ttgaagataa tcaatctgtt tctgaaatga
     2521 tgcagatgtt tttcttaaat gaaggttggg aagcaacttt caagtatgat ggaaaagaag
     2581 ggctcgatgc ctttttagaa aacccacaac attgggatat gattacacta gacctgaatt
     2641 tgcccacact ggacggtatg ctagtaggaa gagagatccg caaagtttca agtactgtgc
     2701 cgattatcat gctgacagct agagattctg aaagcgatca ggtgatcggc ttagagatgg
     2761 gggcagatga ttatgtcaca aaacctttca gtcctctgac gcttattgct cgaatgaaag
     2821 ctttgcatcg ccgtagtgag ttggcagaag caagagaaga agcagtagtt tcagatgatc
     2881 attttgatat cgagactagt catttcaaga tgaatacgaa aacacgagaa gcgtatctgg
     2941 acaaccggcc aattgaagga ctgacgccta aagagttcga tctattgttt actttagcaa
     3001 aaaaaccacg tcaagtattc tctcgggagc agttgcttga attagtctgg gattatcagt
     3061 actttggtga tgaacggaca gtggacgccc atatcaagaa attacgacaa aaaatcgaaa
     3121 aggtcggacc acaagtcatc caaactgttt ggggagtcgg ctacaaattc gatgattctg
     3181 gtgttgccta atgcgttatt tgtatcagca actcttagct ttctgcggaa tggtcgcgtt
     3241 gatcttattg atcgttggag tttcttttac acaactgaca aaacaaacga ttgaagaaaa
     3301 caattaccag caactgtttg gatatgctga atcagttgaa aagacaacgc aaacctatgc
     3361 cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa aatgcattgc tgctgacaga
     3421 gcaagtactg acagaacaaa atgtgaactt tatatttatc gataaaaacg aacgtgtcat
     3481 ttatccgact aatacagctt tactgaattt taccttaaca gctgatcaat ggaaaaatct
     3541 gaggaatgga cgtcaagaaa aattcacatc gaataaaaat attttaggac aagcacaggc
     3601 gacctcttac gcacttgtgc catttaattt gaatcatgaa ttctatggag cacttgtagt
     3661 tagtcaacct gcgcgaaata tcgataacag cgttcgtcca attacactga acctgttcaa
     3721 aggatttatt ttttccagta tcgttgcagt tattgccagc tatgcttttg cagcattcca
     3781 agttaagcgt atcaatcggt taaggaatgc gacgaaagaa gtgacgaatg gtaattttga
     3841 tgtacagctc cccgtacatg acaaagatga atttgatgag ttagcagatg atttcaataa
     3901 aatgacgaat tcattgaaag aatctcaagc agaaattgaa gaacaagaaa atcgtcgaag
     3961 acaatttatg gcagatgctt ctcacgagat gagaacacct cttacgacaa tcaacggact
     4021 tctggaaggg ttagaataca atgcaatccc tgaaaatcag cgagaaaacg cgattaaatt
     4081 gatgaaaaat gaaacagagc gtctgatccg tttggtgaat gaaaacttgg attatgaaaa
     4141 aatccgtacg aatcaaatca gtatggtcat taaaaaattc aatggaacag aaacattgaa
     4201 aaatattgtt gcacagttag aagctaaagc agaagcagca ggagatacat tgacattgaa
     4261 agccgttgat gatattgatg tttacgctga ttatgaccga tttgtccaaa tcatggtcaa
     4321 tattattcaa aacgcgatac agtttactga aaacggccaa atcacgatta ctttagaaaa
     4381 aggctatctt gaaacgatca tcacgattga agatacggga attggaatgt ctgaacaaca
     4441 aatgaaaagt atctgggatc gttattataa agtagatcct tcaagaaaaa atacaaaata
     4501 tggtgaatca ggattaggtt taccaatcgt gcagcaactt gtacgcttgc ataaaggaaa
     4561 attagaagta gaaagcgaac taggaaaagg aacaaaattc gctgttcgtt tgcctgacgt
     4621 agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat catgaatcga tcttctatac
     4681 cgcttgtttt tactataaaa tgattgattt agcccgcggg ctctaaacga tcaatataag
     4741 gactttgagc aatagtagca tcatgagtaa agagtaagat accttgattt tcttcatgag
     4801 ctaaataaga aagcaagtgg agtagctctt ggatctccaa tacactaagt tcatcaaatg
     4861 tatcttcaat aacaatattg tttcgcttgg aaagcaaggc atgaagtaat tgcagcttta
     4921 ctttttcaaa agacgtcaac tcagctgccg cacaattaag caaggaagga gagatatcaa
     4981 gtttttcttg ataggccaaa cgatcttgtt tgatttcttt ttccggtaca tccaacagga
     5041 catggtcttt aagattcaga taaggaaaca tcggccaatt tttttggatg atcgcaaaat
     5101 tatccagttt tgcaacaaga acctcttttt tcaagtgctc caatatttta tgctgttttt
     5161 cgagaggaca caaaataacg gtcaagtgtt cagcatgaat caaatattca ggtttcatta
     5221 cgtccaggcc tcctactttg caaatgatgg ataaaaaaag tgaaagggaa tagactaaga
     5281 atgagacagc atccactgag cccaataaga gtttgggcaa atcccttaag tatatctgtg
     5341 aaaaaaagat cattttggaa atcgatggta aaaaaagtct gttgatccat cggaattgta
     5401 agtccgctta ccgaagtcaa gatatcacgg atatctggca gttcagtagt ctttaagaca
     5461 agaaaattga tcctttgaag aagcgcttca taaactgact ggaagcttac gatccacaat
     5521 aaaaccaaga tacaacaaag aaagagagga aagaacagct gccagaaaat aaaaaggaat
     5581 aatttggttt tagaaagacc agtcaggtgc caattgatca attcctgctt gttttgcctg
     5641 actgaatagt aaaagaaaaa gataaaaagg aaggcaaata gaaaaaataa actgaatatg
     5701 ccttttatat aaaatcggct agctaattct aaagccggaa gaagctctgt agatgcagaa
     5761 gcgccaataa atgaaagccg atcttggact tgattggcta aagccgattg caaatggtaa
     5821 agagtcaggc atgctatcaa taaaaaagca gaaaagcttg aaaataaaca atagagtatt
     5881 gtctgctttt tgtaataaat taatcgagca gtagcatagc ggattatttt catacttttc
     5941 acctctataa aaagtataga aaatgaatat gaataaagta tgaaattcct ttagtgaaat
     6001 cacgaattaa aaccagaaaa tattttgtaa aaagcaagag gttgtgaaga agctgcccta
     6061 ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg agcagcgaca
     6121 ataa
//
LOCUS       DAGCWU010000148         6102 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.149, whole genome shotgun sequence.
ACCESSION   DAGCWU010000148 DAGCWU010000000
VERSION     DAGCWU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.149"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            1..162
                     /locus_tag="MBZ71_002330"
     CDS             1..162
                     /locus_tag="MBZ71_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430242.1"
                     /translation="MKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            159..1463
                     /locus_tag="MBZ71_002331"
     CDS             159..1463
                     /locus_tag="MBZ71_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4430243.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1559..2428
                     /locus_tag="MBZ71_002332"
     CDS             1559..2428
                     /locus_tag="MBZ71_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4430244.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2540..2860)
                     /locus_tag="MBZ71_002333"
     CDS             complement(2540..2860)
                     /locus_tag="MBZ71_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBT4430245.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            3030..3989
                     /locus_tag="MBZ71_002334"
     CDS             3030..3989
                     /locus_tag="MBZ71_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4430246.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            4028..4474
                     /gene="dhaM"
                     /locus_tag="MBZ71_002335"
     CDS             4028..4474
                     /gene="dhaM"
                     /locus_tag="MBZ71_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBT4430247.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            4449..5459
                     /gene="dhaK"
                     /locus_tag="MBZ71_002336"
     CDS             4449..5459
                     /gene="dhaK"
                     /locus_tag="MBZ71_002336"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBT4430248.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            5487..6092
                     /gene="dhaL"
                     /locus_tag="MBZ71_002337"
     CDS             5487..6092
                     /gene="dhaL"
                     /locus_tag="MBZ71_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBT4430249.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
BASE COUNT         2046 a         1103 c         1322 g         1631 t
ORIGIN      
        1 ttgaaaaaaa gacaagaatt acttgaagaa gtgtatacgg aacgttttgg aacaaaagaa
       61 gagagagaaa cggtccgttt ttattctgtc tccgaagaga aaaatctaga tacgacattc
      121 attgcagatt tgtataaaga aaatggggtg gaactgaaat gatctcaatc acccatacag
      181 gtaatttatt cttagatact tgtttgtcta ttatgtattt ttttctcgtt tcttatccga
      241 ttttaggcgg atttgtatgg tttattggtg tctggtgtta tgtgttttta tacaaacata
      301 agcaaaaaga atgggtagat gttcccttga gtgtcgaacc gttcatcacg atcatggtgc
      361 cagcccataa tgaagaaatt gtgatcgaag atacaatcga gtatttgatg acgaaattga
      421 attatcacaa ctatgaggta ttagtgacag acgatggttc aacggaccaa acaccagaaa
      481 tattagctag actgatgaaa aaatacgcta atcttcgtgt ggttcggatc gaaaaaaata
      541 aaggaaaagc ccacgcgttt aatatcggac ttgcctttgc aaaagggaaa ttgattttaa
      601 gcaatgatgc agacacggta ccggagccag atgcactgat taggtatgtg aattatttca
      661 ttcgccctgg tgcaagacat attgctgcgg tcactgcaaa tatggatgtc caaaatcgta
      721 ccaaattgat tgcaaaatct caaacggttg aattctcaag tatcgtgggc atcattaaac
      781 gaacacagtc agctgttttc ggtggtcttt atgcgtacag tggagcaaat acgatgtatc
      841 gaaaagaagc cttgatcgat gtgggcggat tcagacaaga tcgagcgaca gaagacatca
      901 gtattgcttg ggatcatcag ttaaatgact ggttatctgt ttttgctcct gagattatct
      961 ttttcatgga agtacctgtg acgttgaaga tgctatatcg acacagaaaa cgttgggcaa
     1021 aaggtggaac agaagtctgg ctgacgaatt tcaaaaaagt gatgctccat ccttttaaac
     1081 atattggaag aacgatcatc ttcattgatc aaacattgag tattgtctgg tcaatattct
     1141 tttgtatttc cgtagttctt tttgctggat taatcgggca ttatgtatat caaggaaact
     1201 acgaacagat ttatattact tttacattca gttttgtgtt catctgcttt gaaatggtcg
     1261 caggattttt ccagttgcta gcttcgctga ttgtagatga tcgacgaaga aaattgaaat
     1321 accttctttt cgcaccacta tatatgcttt tgttctggat agtgaacgcg ataacgatcg
     1381 tgacgacttt cattccagca gtcaaaacga ttttaggtta tggaagcggg acatggaaaa
     1441 gcccggaacg tacaaaaaaa taaaagatgc accgtttttt agacaaacag cctgttgtgt
     1501 ttatggttct tccaaacatt tttgcttata ataagaaatt agttaaaaag tagtaaggat
     1561 gggaaaagtg atgaaaaaaa gaatgaagcc gttaatctgg ccaattgctg tttccagtat
     1621 tttcttattg ggacagacag cttttgcgga aacttctgaa cctgctcaaa cagaaccttc
     1681 aacaaccata agctcttctg gtgctgaaaa cggtgcctcg gaaacagaaa tgacttccaa
     1741 tgtaccgaca gataattcag gagaaacgat ttcatcttca gaagatacat caataaatag
     1801 ttctacctct tctgaccaaa tcgatcattt gcaagacttg gaaaataaaa gaaaagaact
     1861 cgaagaagca ttagaagctg caaaaaacaa attgaaagaa gaccaagcag caatcgatca
     1921 agatttagct gatcttaaaa ataaagtggg aaatgaccgt gcacaacttg agaaaaagct
     1981 tgccgaagcg aaagcaaaag cggaagctgt ccaaacagat cttgacggga agatccaaca
     2041 agcaaaagaa ttcaatcagc aattaaatca ggttgctcaa acagcttctg gaaagctaag
     2101 tcaaactcaa gcggatatca cagcggccag tcaagcaatc cacggaaaga tcagcgaagc
     2161 acaaacagtt gctaatgatt ttgaagcaaa aaaagctgac ttagcagcta gtttccaacg
     2221 aataaaagat attttgcatt cggcaggaga aaaatcagtg acctctggaa gcacgacaga
     2281 aacagttcag caaacgacag agccatccaa atcaacaagt cagactttgc caaaaacaaa
     2341 tgaaaaaaca gcttctgtca gttattcctt agcaggggcg ggtgtgttag tcgcagcagc
     2401 ttacggaatc atccgcaaaa agaaataaaa aagagaggat gggaaggtag agtacctcga
     2461 agttagactg aaaaatctaa ttcttgagtg ccactacatg tcccatctct ctttagttat
     2521 taaaacagat gtcccctact tatctcgtgg agacagctgt tccactacat gtgaccatca
     2581 gcatgccatt gtcgctgcct aatacttcat agtccatttt tacgccaatg atcgcatcag
     2641 ctcctaatgc ttttgcacgt tcggccattt cggcaagtgc ttcttctctt gcacccatta
     2701 gttcgttttc atagcttttt gagcggccgc caaagatatt tcgcaacccc gcctccaagt
     2761 ctttaaatgc atggatccca gtaatgacct caccaaaaac gatcccttta taagctacta
     2821 cggcttttcc ctctacactg ttcgttgttg ttgtaatcat tcgatcactc ctctctttta
     2881 attaaagtat aacaaacttc aagaagcact acacatattt tcccaaaatt agaaatcgcc
     2941 gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca aaatagttcc
     3001 gtcgaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca ccaactgaac
     3061 tcgtaatgat agaagcttat ttcaatcaat cacactctgt ttcaaaagta gcccatctgg
     3121 tacaacgttc tcgacaaacc atttataatg tctatcgttt tcttaaatct ggtggaagcg
     3181 caataagcta ctatgaacaa tataagaaaa acaaacgtaa ctgcggaaga cgtccgattg
     3241 tgttaccaga tgggcaacaa gaatacattc aaaaaaaggt ggctcaaggg tggactccag
     3301 atgtccttat tggtcgagca gagtttccta ttgcttgttc ggtacgtaca ctatatcgga
     3361 tgtttaaaga taaaaccttt gaccctcacg atttaccaat gaaagggaaa agaaaaccaa
     3421 acggacataa agaaaaaaga ggaaaacaag cttttcgccg ttcgattcag gatagaacaa
     3481 ctgattatat acagttcaat catgagtttg gtcacctaga aggtgacacc attgtcggtg
     3541 gaaaacataa aagtgcggtg atcacgttag ttgagcgatt atctaaagtg atcatcacct
     3601 tgaaacccgc tggtaggcaa gctatagata ttgagaatag actgaatcaa tggtttcaat
     3661 ctgtaccgaa acacttgttt aaatccatca catttgattg tggaaaagag ttctcgaatt
     3721 ggaaaaagat tagtaatacc aatgatattt cgatttattt cgctgatcca ggaacacctt
     3781 ctcaacgagg actaaatgaa cattcgaatg gcttattacg taaagatggc ttatataaag
     3841 ctatggattt taataaggta tccgaaacct ttatccaatc ggtggcatca aaacgaaatc
     3901 atattcctag aaaatcatta cactatcgca caccattgga agtatttttg agttacgtag
     3961 gtaaagatga tttgtctagc ttaatttgac aaacgaaaaa tttattatat acttgaattg
     4021 ttcattaatg aaagcgaaac caatcgttag caactatgta aaagaaagga agattcttgt
     4081 gaaaaaaagt attttgctta tttctcatag tcaaaaagta acagatggta ttaaagaaat
     4141 gattgagcag atgcaaaaat ccgaagaggt gcaaattttt tctctcggcg gaataaatgg
     4201 cgagatcgga tcagacccga tgaaaatcgt agaagcagtc aatgaatcaa gcgatgcagc
     4261 tagttatttt gtttttgcgg atatcggtag cgcggtattg aactcggaac tggcaaaaga
     4321 catgttggaa gaagagcagc agcaacgtta tcatctggtg gatgcgcctt tagtggaagg
     4381 agcttttgca gcagccatca ctgccggtat aacagatgat actcagcaaa tcttaacaga
     4441 agcacaaaat gccggaaaga aaggatggga ataaattgaa aaaaatcatg aatgatccaa
     4501 gcaatattgt cgaagaaatg ctggaaggat tagtaaaaag ctatccagaa cttgttcatc
     4561 gtgtagaatc ttcccgtgtt gtagccaaga atcagaaagc ggaacaagtt ggtttggttt
     4621 ctggtggagg aagcggacat gaaccatccc atgccgggtt tgtaggggaa gggatgttaa
     4681 gtgcagcggt gcttggtgat gtgtttactt ctccaactcc agatcagatt cagacggcta
     4741 ttaaggaagc cgatagtgga aaaggagttt tgctgatcgt taagaattat acaggggatg
     4801 cattgaactt cgatatggca aaggaattag ctgctatgga cgatattgaa gtagaaagtg
     4861 tgatcgtaga tgatgatatc gccgttgaaa atagtacgta tactgctgga aaacgcggag
     4921 ttgcaggtac tgtccttgta cacaaaatcg tgggcgatgc agcaagaaac ggtgcttccc
     4981 tagctgagtt gaaagaactg ggagaaaagg tcgtacaggc aactaagacg atcggtctag
     5041 cgcttcgagc agcaactgta ccagaagtag ggaaaccagg attcgaacta ggagaagatg
     5101 agatcgagta tggtgttggt atccacggtg agccaggcta tcgtcgagaa aagatgcagc
     5161 cttctaaagt actggcaaag gaacttgtta ctaagatttt agatgattac tcgaatttac
     5221 caaaagaagc aggtgtacta gtcaacggaa tgggcgggac gcctttgatg gaacagttcg
     5281 tgttcatgaa tgatgtttta gctctgttgg aagaaagagg cgtcaatgta gttttccgaa
     5341 aagtcggtaa ttttatgacc tcactagata tgcaaggtct ttctctaaca ctgatcgact
     5401 tgacggagac acaatggaaa gattctttag aaagcaatgt acagacgatt tcatggtaaa
     5461 aaatctcaag ataaaagggt gataatatga aactaaccgt tcaagaaatc caagaatggc
     5521 tcacgcaatt tgcagaggca atcaatcaga acaaacaata tctcagtgat ctagataccc
     5581 cgattggcga tggagaccat ggaaataaca tgggaagagg ggtttcagct tatgaagaag
     5641 cttttcaagc cgatcatcct gaaacaatca gcgatacatt caaagtattc tctatggcga
     5701 tgatatcgaa agtcggtggg gcttctggtc cgttatacgg atctgcattc atgaacatga
     5761 tgaaagcgac aaaagaggtc gattctattg attctcaaga aaaattaggc gaagtgatcg
     5821 agcagggaac agcgggtatt caaagccgag gaaaggcaga agctgacgat aaaacgatgt
     5881 tagatgtttg gctgccagtc acagaggcat tgaaggcagg gaatttaaca aaagaaatca
     5941 ttgaccaggc aaaagaacac accaaagtct tggtagctaa aaaaggacga gcttcctatc
     6001 taggagagcg tgcaatcgga catatcgacc caggagctgc ttctagtgcc attttatttt
     6061 caacgctttt agatgtgatc aatcaacctt aagttcaata aa
//
LOCUS       DAGCWU010000149         6026 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.150, whole genome shotgun sequence.
ACCESSION   DAGCWU010000149 DAGCWU010000000
VERSION     DAGCWU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.150"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            57..716
                     /locus_tag="MBZ71_002338"
     CDS             57..716
                     /locus_tag="MBZ71_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBT4430250.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            801..1706
                     /locus_tag="MBZ71_002339"
     CDS             801..1706
                     /locus_tag="MBZ71_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBT4430251.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(1708..2340)
                     /locus_tag="MBZ71_002340"
     CDS             complement(1708..2340)
                     /locus_tag="MBZ71_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBT4430252.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2491..2616
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2660..3184
                     /gene="infC"
                     /locus_tag="MBZ71_002341"
     CDS             2660..3184
                     /gene="infC"
                     /locus_tag="MBZ71_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBT4430253.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3256..3456
                     /gene="rpmI"
                     /locus_tag="MBZ71_002342"
     CDS             3256..3456
                     /gene="rpmI"
                     /locus_tag="MBZ71_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBT4430254.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3509..3868
                     /gene="rplT"
                     /locus_tag="MBZ71_002343"
     CDS             3509..3868
                     /gene="rplT"
                     /locus_tag="MBZ71_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBT4430255.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4304..4645
                     /locus_tag="MBZ71_002344"
     CDS             4304..4645
                     /locus_tag="MBZ71_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430256.1"
                     /translation="MLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLIRKV
                     VELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVKSTILCKS
                     STSSKAQCFAL"
     gene            complement(4752..5939)
                     /locus_tag="MBZ71_002345"
     CDS             complement(4752..5939)
                     /locus_tag="MBZ71_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBT4430257.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         1927 a         1107 c         1218 g         1774 t
ORIGIN      
        1 tttaaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
       61 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
      121 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
      181 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
      241 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
      301 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
      361 tttgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
      421 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttagagcgta
      481 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga cccaagtcta tacgattatt
      541 acaaagaatt gaacagacgt tatgatcaat ggtatgaaga atacaaagaa agtccaaaaa
      601 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
      661 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtatcc atttgattta
      721 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
      781 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
      841 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
      901 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
      961 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
     1021 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
     1081 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cggatgccag
     1141 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
     1201 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
     1261 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
     1321 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcactggct atttatcgca
     1381 agttgtcaga aaaggaaaaa gaagagttgg acgatgcact gatcgaagca cgacaagcag
     1441 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
     1501 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
     1561 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
     1621 ccgttttcgg atgggaaatc gccttgatca tcagtttggt cttatctatt tggatgctta
     1681 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
     1741 gatcacaagt gcttgctctt gattgacgat tagtcccgcc aaagaatctt gagataaagc
     1801 gattgttgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
     1861 cgaaccggtt aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
     1921 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
     1981 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
     2041 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
     2101 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaacc
     2161 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
     2221 gagatagctt tggttacctc cgttgatcag aagaccagag aagatctcct catcttctaa
     2281 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
     2341 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg ctagaaagcc
     2401 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
     2461 attagttttt gctatactat aatggttgag aaataaaagc agaagcaccc gcttctcgcc
     2521 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
     2581 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
     2641 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
     2701 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
     2761 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
     2821 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
     2881 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
     2941 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
     3001 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
     3061 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
     3121 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
     3181 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
     3241 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
     3301 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
     3361 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
     3421 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
     3481 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
     3541 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
     3601 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
     3661 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
     3721 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
     3781 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
     3841 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
     3901 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
     3961 ataacgtata agtctaaaat aacataatta aggtttttta attgaaaact gaacatttat
     4021 ggtcatcctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
     4081 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
     4141 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
     4201 atggtcggct tttgtcatca tggctactgt atggatggga ttagcgctag tcggaaacac
     4261 gttttcacca aactcggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta
     4321 tacgacgggg ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt
     4381 tttcgggcgt gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga
     4441 acttggaggg gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat
     4501 attcatgtcg atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccgat
     4561 cttgatgtca ttaggtattt cagtcaagtc cacaatcttg tgtaaaagtt caacctcatc
     4621 aaaagcacaa tgttttgcgt tgtgaaccta actgccatgc ctttctttct gaggagagag
     4681 gtcaggagca gtagcgcagc gactcttgat gtctctcctc agaaagaaga gactcgcaca
     4741 ggttcatggt gctaaatgtt attcgaatta aattctaaat attttcgtgg agaagccagc
     4801 cactcagtgt ctttttccat caaaagtgtt ccaattatcc gagtgatgga attgatattt
     4861 ggaaaaatcc gaatcactcg ttctctccgg cgaatctctt cgttcaaccg ttccaacaca
     4921 ttggttgtac gaatccttcg gcggatattt tcaggaaaag acaaaatttg aatagcatct
     4981 tcaaagccgt tttctaaaat gtcgcaagca gcacttaatt ttgaatcaca accatatttt
     5041 tccaagaagt gctcttttct ttcacgtgtc aattcaagtt cagaggcttt aaagatactc
     5101 ttcaactcgt cttttacatc agaagaaact ttctttggca gcttatcaaa aatatttctc
     5161 aaaaagtggg cttggcatct ttgccagctc gcacctaaaa agttcttgcg aacagcctta
     5221 actaaccctt tgtgtgcatc agaaatgacc agttttacac cgaacaaacc tcgttgaacg
     5281 aacgattgaa aaacggtatc ccaattatct tccgattcgc cgtcttgaac atcaaaacca
     5341 agaatcgtac gtcgtccatc agaatcaatg ccgataccaa tatatacacc ttttgagaca
     5401 atacgatgat tttcccttac tttcatatac agggcatcac acatgaaaaa tggatattcc
     5461 gagccttcta ggctccggtt tctccactca aaaacaaatg gatccaactg tttcatcaga
     5521 tttgaaacga atgatttaga aacagtcgca ccatccgtta gcagttcaac tgttttagtt
     5581 actttgcgag tggagacacc agaaatgacc atttcaatca tggcagcgat caaagctttt
     5641 tcgcttcgct gatatctttc aaagatagta ggactaaact taccgtctct ggttcgtgga
     5701 acatgaagtt ctaacgtacc aattcgagtt gtaaacgaac gatcgtagta accatttctt
     5761 tgtgattgcc gttcttctga acgttcatag gcatttgcct gaatatattg atttcgttgt
     5821 tcttccatca gctgattaaa catgatagtc ataagtgttt ttgcggtatc attcgcaccg
     5881 ctttcgttta taattgcttg aatttcttcg ctttcaaaag taaaatgtac ttgagtcatt
     5941 gatatcagtc ctattcgagt atgttttctg caaaaaacat tgtactccta actgagatca
     6001 atggcttttt tgatgcttca ctttta
//
LOCUS       DAGCWU010000150         5970 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.151, whole genome shotgun sequence.
ACCESSION   DAGCWU010000150 DAGCWU010000000
VERSION     DAGCWU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.151"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..453)
                     /locus_tag="MBZ71_002346"
     CDS             complement(<1..453)
                     /locus_tag="MBZ71_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4430258.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITNY"
     gene            677..1468
                     /locus_tag="MBZ71_002347"
     CDS             677..1468
                     /locus_tag="MBZ71_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4430259.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1434..2387
                     /locus_tag="MBZ71_002348"
     CDS             1434..2387
                     /locus_tag="MBZ71_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBT4430260.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2785..3759)
                     /locus_tag="MBZ71_002349"
     CDS             complement(2785..3759)
                     /locus_tag="MBZ71_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4430261.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YNAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3874..4308
                     /locus_tag="MBZ71_002350"
     CDS             3874..4308
                     /locus_tag="MBZ71_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4430262.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4433..5455)
                     /locus_tag="MBZ71_002351"
     CDS             complement(4433..5455)
                     /locus_tag="MBZ71_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBT4430263.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5847..>5970
                     /locus_tag="MBZ71_002352"
     CDS             5847..>5970
                     /locus_tag="MBZ71_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HBT4430264.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETH"
BASE COUNT         1877 a         1125 c         1027 g         1941 t
ORIGIN      
        1 ataatttgta ataagttcgt atatgttttg cggtgtttct tgtttcttgt aattttccac
       61 gaatgtttca tccatgaata aattcataaa cgcttgaagt aatcctactt gttcttttgg
      121 ttctttgatt cctaaaaaaa atagattttc tacagtcatt tcctgattat cccccatttg
      181 ataaaaataa attgggttct tcagctgcat aacaaaaaca aatggctttt cgacatattc
      241 acattccgaa tgtggcagcg cgatatttaa atacttagta atcaatccag taggaaattc
      301 tctttctctt gcaatcagtc cattcaaata attctcattg acgtacccca ttttttctaa
      361 ttgttgtgca gcgattaaaa aagcttcttc tttatttttt acttctaccg ctaattgaat
      421 caaactagga acgaacatct ctttatattt cattccaacc tcctcctttt tgattaagtt
      481 tgttaattta ttgttctact aagttcatct ttgttttaca agaacattat atttctgtta
      541 gtttgttttg tcaacgcttt caaaagaaat ttattttgtt aaatttgatc tacttgaaaa
      601 taaaatcgtt tatctatata attaagaaga acaaagtcga acaaaaattg acttttatgt
      661 taaaggagta attcatatgc tgaaaaaaga aagacaagaa caaatcatta cattattaga
      721 gaaagatttt tatttaacta attcagaaat cgctaacaaa ttaggcgtat cagaaatgac
      781 catacgccga gatgtcactg aattagcaaa aaaaaataaa gtaaaaaagc tatatggtgg
      841 cgttgaacga aaggaacctt ataatcgaga actatcgact caagaaaaga ttgatacgaa
      901 tgtagccgaa aaaaaatata ttggtcaagt catgaacagt atgattcccg ataattctac
      961 tatttatgtc ggtgctggaa caacgatcct atatgcactt cctttcattc aaaagaaaaa
     1021 tctatttgta attacaaaca gtttgattgc tttcaactat ttaaagaatc atacaagtta
     1081 tcggatttta cttactggtg gcgaattttc tcctattaca gaagagttca ttggggaagt
     1141 tgcagaaaga tcttttgaaa gtttgaatgt cgatatctct ttcgctgcaa caaacggcat
     1201 atttgataac aatgtgacaa ccgctcaatt tattgaggga aatattcaga acgcagcgct
     1261 caatgcagca aaaatcaaat gcattgttgc agattattca aaattaaata aatcagacgt
     1321 ctatactttt agaaaattaa ccgattttga ttatctaata accgataatc atttatcaga
     1381 acaggatttc aatcattatt caaaatactt aacaatatta aaggagccca aaaatgatac
     1441 tgacaatcac gatgaaccca tccattgatt ccatttattt tacaaaacac tttgaattag
     1501 gtacgatgac acgctttgat tctccggtac gctatgtagg cggcaaggga atcaattgcg
     1561 gacgagcttc tgctctacta ggttcatcag ttattttaac agggtttttg ggcggttatt
     1621 ttggcagtat gattcaagat atgctgaaaa atgaaaattt gtttgatttg cattttttac
     1681 ctattgcaga agaatcaaga ggtgccgtca cgatcatgca tgatgatgaa acgcagacag
     1741 agctagtgga agctggtcct gaaatagcag atactactat caaaaaatta atgaatgata
     1801 tcaaactaat cagtaaaaat aattaccttt cagtcattac aataaatggt tctgttaaca
     1861 atccagatcc ctttttgtat gctaaattat tacaatatat caaagaacaa atagatcctt
     1921 ccacctatat attaatggat atatctggac aacatctccg tgaagtctta gcaagtaagt
     1981 ataaaccgat gttcatcaaa cctaatatcc atgagttttc agatatcatc catcaaaaaa
     2041 ttactcaaaa aagcgacata aaacaccatc taaaaacaaa tccactattt aaagatatcg
     2101 atgttatcat gatttcttgc ggctcgtatg gtgcagtagt caaagtaaaa gaagaatttt
     2161 ggaatgttga gatccctaag attcctatca ttaatacaac aggctctggg gatgccactg
     2221 ttggaggttt tgctcattca ttagaacaga attatccttt aaaagaatgt gtaaggtacg
     2281 caatggcttg cggcatgtcg aatgcacagc aatcagaagt cgggaaagtt attccagctg
     2341 atgtaaaaaa tttagcaaaa agcattaaaa tcacagaaat gaaataaata gtacaaagta
     2401 aataattgtt tatatgaaat gagacacaaa gagactgtga caaaaaacgt gtgtaccgac
     2461 ccccaaaagt tagatttttt ggtctaactt ttgggagtcg atacattgtc acagtccttt
     2521 tttcgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat attcaacaaa
     2581 catgagaagc tattttttga aattgctcct tgtgactcag agttaaacgg caggctcacg
     2641 acctcttggt ataaacggtg ttcaaaagct tgcatctacg ctaatgagtc caaccaagtc
     2701 aaaaatgagg agcttttttg gctgtcctta ttacttgatg cagggcagtt ttttcataat
     2761 ctcttttaga ttaaatcctt ttggttacac aaggttctct tcaaagaatg ttgccatttt
     2821 ttcaaatgga atgatgtctg tctggtcgta tagatctgta tgggatgctc ctggaatgat
     2881 catgagttcc ttgttgtcac cttttagttc tttgaaagcg tcctccccaa agtaacgtga
     2941 atgagctttt tccccgtgaa tgactaagac tgcatgacgg atttctggaa tataatgcaa
     3001 tagttttgca ttcataagtg acaaccctga tgtcctattc caaccatcat tggagtttaa
     3061 agaacggaca tgatatccgc ggtctgtttt ataatacgca ttatagtctt tgacaaattg
     3121 aggagcatct tcaggcaacg gatcaaccac acctccagca cgtgcgtagt ccccttgttt
     3181 gaaatcaatg atgcgttgtg catttaattc ttcacgcatt tcatatcggg cattttcatc
     3241 cataacatcg aagtagccat ttgctgttac tcgagtcatg tcatacattg tggaaacaac
     3301 tgttgctttg acccgcgtat cgatagctgc tgcattcaat gccatacctc cccatccaca
     3361 aataccaatg attccgattc tctcagggtt tacatttttt tgtacagata aaaagtctac
     3421 tgccgcttgg aaatcttccg tattgatatc aggcgaagca acatatcgag gtactcctcc
     3481 gctttctcct gtaaacgatg gatcaaacgc aatcgttaaa aagcctcttt cagccatcgt
     3541 ctgcgcatat agaccagacg attgttcctt cactgcacca aatggcccgc tcacagcgat
     3601 tgccggcaat ttcttttcac ttacttcttt tggttcatat aaatcagcga ctaatgtgat
     3661 accaaaacga ttcgggaagc tcacctttcg atgattgact tgattgcttt ttggaaatac
     3721 tttgtcccat tcttgtgtga tgtttaattc tttattcata ttctagactc tcctttgatc
     3781 aaaattgaca tttgtttttg aatgtattat tatgataaaa cttaaacttg actttaggtc
     3841 aaggtaaaaa ttttatttta ggaggatttt tttatgtatt ccattggaca agtgtctgaa
     3901 atgtttggat tgcctgtttc tactttacgt tattacgaca aagagggatt atttcctgat
     3961 atccagcgtg tttctggtat tagaaaattt tcagagaatg agttagaggc tttaagagtc
     4021 attgactgtt tgaaaaaatc tggtttagaa ataaaagaca ttaagcaatt catggaatgg
     4081 agcaaagaag gcagtaagac atttgaggtt cgtaaagaac tatttgaaaa acaaaaagaa
     4141 gtagtagaaa aagaaatagc aaaattagaa agagtactcg ctatgctaaa ctacaaaagc
     4201 tggtactatg aagaagcaat aaaagccggc aatgaagaag ccgtattgac aatgattcct
     4261 gatgatttac cccaacatgt aaaagaagct tatgtacatt ctcattgaac aaacggataa
     4321 ctcactctcc ttctctatga acttcatgaa aacatgaata aacacctcta actgattgtt
     4381 agaagtgttt attttttcac tcaagtatcc tgcacttaca aaaatatcct ttttaccgcc
     4441 atcctaattc tggagcgata tcttttacga ttgtttcaag caagtgcatg ttatattcta
     4501 cccccaaagt attaggaatc gtgacaagta ctgtgtctgc ttctttgatt gcttcatctt
     4561 cagctagctc cttgattaat tgatcaggtt caccagcaaa agtttttcca aaaatcgttg
     4621 ggtttcggtg ataagcaaga tagccgacag catcttgttg cggactaccg tcttgtccaa
     4681 ataagcgtcg atccaaatca gtggtgattg gctgaatcga gcgagtaact aatgttcttg
     4741 gttcaaaatc atgacctgct tgcttccacg cttctttata cgcacgtaat tggtttgctt
     4801 gttggatatg gaatggctcg tgggattcgt aattgactaa tgtagatgat tgcagattca
     4861 ttccatgttg tgctgcccag attgccgttt gctgcgaacc agctccccac cagattcttt
     4921 gtcgtaatcc ttctgaataa ggttctatcc gtaattttcc tgatccttgt gggaacattg
     4981 gttgaggatt tggttcttca aatggttttc cttcaagtaa ttccaagaat tcaagggtgc
     5041 gttcccgtgt tacctctttg atttcttctt cactgtaatc ataaccaaag tatttccagc
     5101 catctagaac ttgttcaggt gaaccacgcc ccactcctag ttgcactctt ccttgcgtga
     5161 tgatatctgt caatccggca ttttctgcca tataataagg gttttcatat cgcatatcaa
     5221 taagtcctgt accgatttca atcttacttg tctttgcgcc gattgccgct agcaaaggaa
     5281 acggcgaccc aatctgaggg gcaaaatgat gtacgcggaa aaacgctccg tccactccga
     5341 ttttttctgc ttccaccgct aaatcaatcg attgcaaata agcatcttga gctgatttca
     5401 caagcgatcc ttgctccatc caatgtccaa agcttaaaaa gccgattttt ttcaaggtaa
     5461 tgacctcctt taaacctaca aaaatggaag gttttttatt tttaatatga actgattcta
     5521 ctactttttt tcttaaaaga cgaataattg ttctcgcctc acaatcaaga aatgtatcgg
     5581 cttactacac ccctactttc ttcatcatct gccgtgtctt aatctctttt aacagtctgt
     5641 tacatcgttg atttttgact caaaatgatt gtttctaaca tgtatttatg gtaaaaaaga
     5701 caagattagt tctttgtaca catcgattta tacgctgaac aaaaagaaat atataaacta
     5761 agaaacccat tgttttttcg ctccatcaca tagagtttac tttagataaa cagtgacatt
     5821 tactaatcca taacggagga aatcaaatga aaaaaatcta tttcttatgt acgggcaatt
     5881 catgccgcag tcaaatagca gaaggctacg cgcgtaaata cttgtctcct tcaatgtttg
     5941 agataagaag tgcggggatc gagacacatg
//
LOCUS       DAGCWU010000151         5936 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.152, whole genome shotgun sequence.
ACCESSION   DAGCWU010000151 DAGCWU010000000
VERSION     DAGCWU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.152"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            361..642
                     /locus_tag="MBZ71_002353"
     CDS             361..642
                     /locus_tag="MBZ71_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430265.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            644..1060
                     /locus_tag="MBZ71_002354"
     CDS             644..1060
                     /locus_tag="MBZ71_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430266.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1682..2836
                     /locus_tag="MBZ71_002355"
     CDS             1682..2836
                     /locus_tag="MBZ71_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBT4430267.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2830..3252
                     /locus_tag="MBZ71_002356"
     CDS             2830..3252
                     /locus_tag="MBZ71_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430268.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4422..5390
                     /locus_tag="MBZ71_002357"
     CDS             4422..5390
                     /locus_tag="MBZ71_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4430269.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            5407..5931
                     /locus_tag="MBZ71_002358"
     CDS             5407..5931
                     /locus_tag="MBZ71_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBT4430270.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
BASE COUNT         2252 a         1014 c          962 g         1708 t
ORIGIN      
        1 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
       61 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
      121 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
      181 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
      241 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
      301 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
      361 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
      421 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
      481 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
      541 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
      601 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
      661 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
      721 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
      781 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
      841 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
      901 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
      961 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     1021 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     1081 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     1141 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttgaggcg
     1201 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     1261 ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat cctattcatc
     1321 aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat atcaaatcct
     1381 ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa ctaaaaaaga
     1441 gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt aaaaatcaca
     1501 aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtgtaca ttatgttaac
     1561 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     1621 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     1681 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     1741 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     1801 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     1861 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
     1921 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
     1981 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
     2041 agaatattca ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag
     2101 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
     2161 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
     2221 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
     2281 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
     2341 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
     2401 agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat
     2461 gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga
     2521 tcaaataata aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact
     2581 aaacctttca gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc
     2641 tggttatgga aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca
     2701 aggtggttat caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag
     2761 tgcaaatgca ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac
     2821 cattacaaat tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc
     2881 ttaactagtt gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga
     2941 acttggcaaa ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa
     3001 aatatagaac aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat
     3061 aaaaatttaa atataaatga acaagttttc atttacaaag caacaaataa tttactaaca
     3121 ctgcgctcta ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc
     3181 gtatttattt tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt
     3241 aaagctgatt gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa
     3301 caagcgaaac tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt
     3361 gggttttgag ggggattcaa gggggagctt gcgccccttt gaaaagcggt tatttgcacc
     3421 aaatacgtag gtatttggct agcaaatata gagcttgcca aacactttag tgagttttgt
     3481 agaaagtacc tttaaaggta ctttcacatt acgaacgata cgtccgtagg acgtactgaa
     3541 agggtgggat ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata
     3601 tatgctaata atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag
     3661 ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca
     3721 cacgagcaaa tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag
     3781 tatataaata tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag
     3841 acaactttag ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat
     3901 aagggattta tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa
     3961 gagttcctta tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag
     4021 agcagagcga tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa
     4081 tgcgcgaatc tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct
     4141 cttttataat ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac
     4201 tctcccaagg gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg
     4261 tctgtttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga
     4321 attagtacat cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa
     4381 cgtgtatctc ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa
     4441 agtatcatca tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt
     4501 tgttcttttc tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat
     4561 tcgagcaatt aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg
     4621 atttagaaac aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg
     4681 gtttgcaacg tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata
     4741 ttccttttgc tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag
     4801 aatgggtgcg ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa
     4861 tgtttcgatt actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact
     4921 ttcatgaatt acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt
     4981 tcaatccaat acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat
     5041 acggaaaagg acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa
     5101 aaaaatcaga cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag
     5161 aactagctaa ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca
     5221 aagaacaaaa gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa
     5281 atacccaaaa taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag
     5341 aactaaaaaa agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta
     5401 gattttatga cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca
     5461 gcaattaaca aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac
     5521 aagtttgtga ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac
     5581 gaaaactcaa atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc
     5641 aaccaagttc ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat
     5701 atgcaactac aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa
     5761 caacaactca ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg
     5821 acttatacgg aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg
     5881 atagaaaaac aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaacca
//
LOCUS       DAGCWU010000152         5788 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.153, whole genome shotgun sequence.
ACCESSION   DAGCWU010000152 DAGCWU010000000
VERSION     DAGCWU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.153"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            131..1075
                     /gene="mvk"
                     /locus_tag="MBZ71_002359"
     CDS             131..1075
                     /gene="mvk"
                     /locus_tag="MBZ71_002359"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBT4430271.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1068..2045
                     /gene="mvaD"
                     /locus_tag="MBZ71_002360"
     CDS             1068..2045
                     /gene="mvaD"
                     /locus_tag="MBZ71_002360"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBT4430272.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2058..3143
                     /locus_tag="MBZ71_002361"
     CDS             2058..3143
                     /locus_tag="MBZ71_002361"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBT4430273.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3140..4204
                     /locus_tag="MBZ71_002362"
     CDS             3140..4204
                     /locus_tag="MBZ71_002362"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBT4430274.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4453..5256)
                     /locus_tag="MBZ71_002363"
     CDS             complement(4453..5256)
                     /locus_tag="MBZ71_002363"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBT4430275.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5276..5581)
                     /gene="kduI"
                     /locus_tag="MBZ71_002364"
                     /pseudo
     CDS             complement(5276..5581)
                     /gene="kduI"
                     /locus_tag="MBZ71_002364"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(5563..>5788)
                     /locus_tag="MBZ71_002365"
     CDS             complement(5563..>5788)
                     /locus_tag="MBZ71_002365"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBT4430276.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         1744 a         1239 c         1198 g         1607 t
ORIGIN      
        1 tttagtaaaa taaaggagga atgttttttg agacccttag tggacaaaaa aattcgtgaa
       61 gcagcgcaac tcgttttggg cgctttattt tttgcttaaa aatcactttt acgtgagaag
      121 gagttagatt atggcaaact atggccaagg agaatcaagc ggaaagatca tattgatggg
      181 cgagcacgcg gttgtttatg gagaaccagc gattgccttt cctttctatg caacaaaagt
      241 caccgcattc cttgaagagc tggatgcaat ggacgatcaa ctggtttctt cctactattc
      301 aggaaattta gccgaagctc ctcatgcatt aaaaaatatc aaaaaattat tcattcactt
      361 aaaaaaacag catgacatcc aaaaaaactt gcaactgacc attgaaagca cgattcctgc
      421 tgaacgtgga atgggatcaa gcgctgcagt cgccacagca gtcactcgtg ctttttatga
      481 ttacttagca tttcctttgt ctcgtgaaat actattagaa aatgtccagc tttcggaaaa
      541 aatcgcccac ggtaatccta gtggaatcga tgcagccgct actagcagct tgcagccgat
      601 ttattttaca aaagggcatc cttttgacta cttttctttg aacatcgatg cttttttgat
      661 tgtcgctgat acaggaatca aaggacaaac aagagaagcc gtcaaagatg tcgctcacct
      721 ctttgaaacc cagcctcatg aaactggaca aatgatccaa aaattaggat acttgacgaa
      781 gcaagcaaaa caagcaatca ttgagaattc accagaaacg ttggcacaga caatggatga
      841 atcacagtca cttctggaaa agctgacaat cagcaatgat tttcttaatc tattgatcca
      901 aacagcaaaa gataccgggg cattgggtgc caaattaact ggcggtggac gcggtggctg
      961 tatgattgca ttagcacaaa caaaaacaaa agcccaagaa atcagtcaag cacttgaaga
     1021 tgcgggtgct gctgaaactt ggatacaagg attaggagta catacctatg tttaaaggca
     1081 aagcacgcgc atataccaac attgctctaa tcaaatattg gggtaagaaa aatgaagaac
     1141 tcatccttcc aatgaacaac agcctttcgt taacattgga tgcattttat acagagacag
     1201 aagtcatctt ttctgatagc tatatggtag atgaatttta tctagatggc accttacaag
     1261 acgaaaaagc aacaaaaaaa gtcagtcagt ttcttgacct ttttcgtaaa gaagccggcc
     1321 tctcactaaa agcttcagta atcagtcaga atttcgttcc taccgcagct ggattagcct
     1381 cctctgccag cgggctagct gctttagcag gagcttgcaa tactgctctt aagcttggat
     1441 tagacgatct ctctctttca agatttgctc gacgcgggtc tggttcagct tgccgaagta
     1501 ttttcggtgg tttcgttgaa tgggaaaaag gccatgacga cttaagttct tacgctaagc
     1561 cagtcccttc cgattctttc gaagacgatt tagcaatggt tttcgttttg atcaacgacc
     1621 agaaaaaaga agtgtccagc agaaatggga tgcgtcgaac agtcgaaaca tccagttttt
     1681 atcaaggctg gttagattcc gttgaagggg atctatatca attgaaacaa gcaatcaaaa
     1741 caaaagattt ccaacttctc ggagaaacga tggaaagaaa cggactaaaa atgcatggaa
     1801 cgactttagc tgcccagcct ccattcactt actggtctcc tgacagctta aaagcgatgg
     1861 atgcggtgcg gcaattgaga aaaaaaggca ttccatgcta ctttacgatg gatgctggcc
     1921 ctaatgtcaa agttttagtg gaaaacagcc atttatctga ggtacaagaa acatttacca
     1981 aattatttag caaagaacaa gtaattactg cccatgctgg tccaggaatt gcgattattg
     2041 aataaaggga gatttttatg attgaagtat ctgcaccagg caaactctat attgccggag
     2101 aatatgcagt tgttgaaaca ggacatccag cagttatcgc tgcagtcgat caattcgtaa
     2161 cagtcactgt agaatccgca cgaaaagtcg gaagtatcca atctgcacaa tatagtggga
     2221 tgcctgtacg ttggacaaga cgcaacggag aattagtttt agatattcgg gaaaatcctt
     2281 ttcattatat ccttgctgct attcgtttga ctgaaaagta tgcacaagaa aaaaacattc
     2341 ttttatcctt ttatgatctg aaagtaacaa gtgagttaga cagttcaaat ggccggaaat
     2401 atggattggg ttctagcggt gccgtgaccg ttgcaacggt caaagccttg aatgtttttt
     2461 acgcgttgaa tttatctcaa ttggagattt tcaagattgc agcactagcc aatttagcag
     2521 ttcaagataa tggttcctgc ggcgacatcg ctgctagctg ttatggtggc tggatcgctt
     2581 tctcaacctt cgaccatcct tggctccaag aacaagaaac tcagcattct atcagtgagt
     2641 tacttgccct ggattggcca ggtctatcca ttgagccatt gattgctcct gaagatttac
     2701 gtttattgat tggttggacg ggtagccctg cctctacttc tgatttggtc gatcaagttc
     2761 accgttcgag agaagacaaa atggtggctt atcagctttt cttaaaaaac agtacagaat
     2821 gtgtcaacga aatgatcaaa gggtttaaag aaaataatgt aacgttgatc caacagatga
     2881 ttcgaaaaaa ccgacaatta ctgcatgatt tatctgcaat cactggggtc gtcatcgaaa
     2941 cgcctgcttt gaacaaattg tgtaatttag ctgaacagta tgaaggagcc gcaaaatctt
     3001 ctggtgcagg tggtggcgat tgcggaatcg taattgttga ccagaaatct ggcatccttc
     3061 ctttaatgag tgcatgggaa aaagcagaaa tcactccact gccgttacat gtctatagcg
     3121 atcaaagaaa ggaaaaccga tgaatcgaaa agacgaacat gtttcactag ctaaagcctt
     3181 tcatgataaa caaaaaaacg aatttgactt cgtacggatc atccataacc ctttgccgca
     3241 aatagcagtt tctgatgtcg atctaagtac acaggcagtt gggtttacac taagcagccc
     3301 attttatatc aatgcgatga ctggcggaag cgaaaaaaca aaaaaaatca accaagatct
     3361 tgcaatcgtc gcaagagaag cagatttgat gattgccact ggttcagtca gcgctgcctt
     3421 gaaagaccct tctttagccg atacttatac gattatgcga caagaatatc ctcacgggaa
     3481 aatcattgcc aacatcggtg cagggacttc tgtcgaaaga gcccaagaag ctatccgatt
     3541 gtttcacgca gatgccttac agattcattt gaatgcacct caagagttgg ttatgcctga
     3601 gggagaccgt gattttacca attggaaagt acttattcaa gagactcaaa cagccattga
     3661 cgtccccctc atcgtaaaag aagtcggttt tggcatgaca agagaaacgc tcaatgatct
     3721 agctgcttta ggagttcata cagttgatat cagcggtcga agcggaacaa gcttcaccca
     3781 gatcgaaaac gcccgtcgct ccaagcggga actgagttat ttagctgact gggggcaatc
     3841 gacagtctct tcattgcttg aagcaaatga agcagacact tctatggaaa tcctagcctc
     3901 cggaggtatc cgcaatgcct atgacatatt taaagccctt tgtctcggcg caaatgcagt
     3961 gggtacttca ggaaccgtac tgacgcatct gatgaatcac ggcgtagaag aaactattat
     4021 actgatgaag caatggcaag aagagcttcg tctgctttat actatggtcg gcgcaacgaa
     4081 taccgcggcc ctacaccaac aatcactgat cttttccgga ccagtgaagg attggtgcga
     4141 agcaagaggg atcgacttag tgaagtatgg aagacgcaca gaaaagagcg tgggacaaaa
     4201 gtaaagaaca cttttatccc acgctctaaa cacgtataaa cggcggaagc aaaagcaact
     4261 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aattccttct
     4321 tatttcttgg agttaaacgc ttctgtccca gcctcttttt tcgaataaat gatgtttaat
     4381 cagctgtcct tcggtattcg ttcaagccgc tgatttcaca acctctccac taaacggtgt
     4441 actaaagctc ttttaaggtt gttttccgat ataggcaagt atgcctccgt ccacatacaa
     4501 gacatggccg ttaacgaaat cagatgcgct agaagccaag aagacagatg gtccagccaa
     4561 atcgattggc tcgccccagc gtgctgctgg cgtacgtcca acgataaatt gatcgaatgg
     4621 atgacgttca ccgttttctt gtgtttcgcg taatggagca gtttgcggtg tagcaatata
     4681 acctggtcca attccattac attggatatt gtattgaccg tattcagaag cgatgttttt
     4741 cgtcagcatt tttaatccgc cttttgccgc agcataagca ctgaccgttt cgcgtcctaa
     4801 ttcactcatc atggagcaga tattgatgat tttaccgccg cctttttcga tcatatcccg
     4861 aataactgct tttgaaacga tgaatggggc atttaaatcc acgtcgatta cttggcggaa
     4921 gtctgccgca tccatttcga tcatcggtgt ccgtttgatg atccctgcat tgttgactag
     4981 aatatcaact gaacctacat cttctttgat ctgttgaacc atcgcttgaa ccgcttcttc
     5041 atcggtcaca tcgcagacat aaccttttgc atcgatgcct gcttctttat aatgcgctat
     5101 accttcatca actgattctt gattcaagtt attaaatacg atttttgctc ctgctgtagc
     5161 taaagagcgt gcaatctcaa atccaatacc atagacagca ccggtgacta atgctacttt
     5221 tccatctaaa cgaaacatat ccatactaaa ttccatcatt ttctctccct tatgcttatt
     5281 ttaattgatc catttgcacc atatccatgt ctgtataggt gatattttct ccgcacattg
     5341 cccagatgaa tgagtaattg cttgtaccta cacctgaatg gatcgaccag cttggcgaaa
     5401 taatggcttg ttcattgtct acgactaaat gttttgtttc atctggtttg cccatcatat
     5461 ggaacacacg tgtatcttca ttggcataat caaaatagac ataagcttcc atacgacgtt
     5521 catgtgtatg acaaggcatc gtattccaag cacttcctgg ttctaaaatc gtataaccca
     5581 tttataaatg acagctatcg ctgaagcttc tactgctgta aatatccccg tcaagatacc
     5641 accgataatg atgacaatca atagaaggct aggaatcgcc tcttttatta tttttccagc
     5701 atgagccatc tgtccttttt cgacaacagg gtatttattc tttttagcaa cgatgaatgc
     5761 tactagcatg attcctagcg cccacaaa
//
LOCUS       DAGCWU010000153         5736 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.155, whole genome shotgun sequence.
ACCESSION   DAGCWU010000153 DAGCWU010000000
VERSION     DAGCWU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.155"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            106..405
                     /locus_tag="MBZ71_002366"
                     /pseudo
     CDS             106..405
                     /locus_tag="MBZ71_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
     gene            complement(764..2668)
                     /locus_tag="MBZ71_002367"
     CDS             complement(764..2668)
                     /locus_tag="MBZ71_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBT4430277.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTDMAPESALVQTEDGEFEEVDVDDVDEGDVLLVKTGAKVPVDGTVL
                     TGEGHINEASITGESVPVTKTEGSEVFAGTILENGTIQIWADKVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKWYTPTVLVLAFIVWAMTQNVELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVINDFSKVDTIVFDKTGTLTVGNPEVAETEVYGDNVE
                     KAYSYLASVETESDHPLAKAVLQHTGTVELFPVEDTDVVKGGGVVAQVDGHRIAVGNV
                     ALMEKENVQLSKKAQKDVKQFEKNGNSLVLTAVDGELKILMGIRDQIRPGVKQDLQEL
                     KNLGVKDLVVLSGDNQGTVDRVARELNLTEAHGHMLPEDKAAYIEKLQQKGHTVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIY"
     gene            complement(2866..3099)
                     /locus_tag="MBZ71_002368"
     CDS             complement(2866..3099)
                     /locus_tag="MBZ71_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBT4430278.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGVDKDSIKVLFNSSKV
                     KANFDSAVTSIEEIQKAIENVGYPVLKAKVKAA"
     gene            3338..4378
                     /locus_tag="MBZ71_002369"
     CDS             3338..4378
                     /locus_tag="MBZ71_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HBT4430279.1"
                     /translation="METERQIRELEKAMNTIDNRRLYERFLAVKLVLEGHTRKETGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKAGSKKQQVFLE
                     ETFPSLKKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIG
                     TLDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLE
                     ENARRLALIFLPPYRPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNER
                     PLEVIDRLCVRM"
     gene            complement(4693..5292)
                     /locus_tag="MBZ71_002370"
     CDS             complement(4693..5292)
                     /locus_tag="MBZ71_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4430280.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLSDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFLEQ
                     LQVQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTVVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
BASE COUNT         1624 a         1253 c         1040 g         1819 t
ORIGIN      
        1 ttttacactc tcatcaaatc attagaatcg tgataagaca ttgtattaaa tctaatcctt
       61 tatccatttt ttatcaatct tacttcgcac catctccaat acttattatt agcctctctt
      121 ggagcatgta aagttattgt agctcggtct ttcgctaaag tgcagggcat aaatttaaac
      181 gatatccgtg tagagataga tggagtatta gatcctgatg gttttatagg aaaaaataaa
      241 gcagctaaaa taggctattc tgcaatcgta tctaaatttt atattgaagc tgataatact
      301 gatgaagaga tttcagattt tatcgcattt attgatcgta cttgtccggt agctgatacc
      361 attaaaaatg cacctacgac taaatcagaa atccataaat tataaactat gaattaaaaa
      421 agattcttct cattttgcac taaacaggta tttctatttt atttcttgtc ttttcgaagg
      481 tatacatatc tataaaaaag acgagagaaa aaaactagtt aaatcaagat tttaaataca
      541 ttattttaag caatttcatg tgttacaact taataggaat ttaaatttta ttattaaatt
      601 ttttaatcat ttacacgaaa attaatttta taaagaacat actctatact cttatttttt
      661 ttcaagaact caaaaaagcg gaaaatccca gatttaaaaa tctgagtttc cgcttttgtc
      721 atttcaagga tggactattg tctcagcctc ttctaattta ttcttaatat atttttactt
      781 tctctgcagg tgctgatact tctttttgtt ctttccgacc tttcaactta tacttcatta
      841 atctcatgcc attgaaaatt accaccaaga tactaccttc atgaaccaac attccgatag
      901 acatattcat ccattcgctg aagaagacgc tggtcaacaa gaccaacact actccaactg
      961 aaatcacgat attttgtttc atattatttg cggtggcttt tactaatcct aatgcgtggg
     1021 gcaagttgct gaagttcgag ttcattaaaa cgacatcgga tgtttcaatc gctacgtccg
     1081 ttccgcttcc cattgcaatc ccgatatctg ctagcgccaa ggacgggctg tcattgacac
     1141 cgtccccgac aaaggcgacc gtgtgccctt tttgctggag tttttcaata taagccgctt
     1201 tgtcttccgg cagcatatgc ccgtgcgcct cggtgaggtt cagttcacgc gcaacccggt
     1261 ccactgtgcc ttggttgtcg ccggaaagaa ccactaaatc tttcacgcct aaattcttca
     1321 attcctgcaa gtcttgtttc actcctggac ggatttggtc gcggattccc atcagaatct
     1381 tcaattcccc gtcgactgct gtcaatacta aggagttccc atttttctcg aattgtttga
     1441 cgtctttctg cgctttttta ctcaactgca cattttcttt ttccatcaac gccacatttc
     1501 caaccgcgat gcggtgtccg tccacttgag cgacaacgcc gccgcctttg acgacgtctg
     1561 tgtcttctac cgggaacagt tctaccgtgc cggtatgctg gaggacggcc ttggctagtg
     1621 gatggtctga ttcagtctcc acgctggcta aataactgta ggctttttct acattgtctc
     1681 cgtacacttc tgtttccgcg acttcaggat tcccgacagt cagcgttcct gttttgtcga
     1741 acacaatcgt atcaactttg ctgaagtcat tgatgacttc gcttcctttc aaaagaactc
     1801 cattgcgtgc accgtttccg attccggcta cgttggagac cggaacccca atgaccaatg
     1861 cacctggaca gcctaggact aagatcgtaa tcgccaattc aacgttttgg gtcattgccc
     1921 agacaatgaa tgccagaacc aaaacagttg gggtatacca tttagaaaag cggtcaataa
     1981 agcgctctgc ttctgattta gaatcttgcg cttcttccac aagttcaatg attttaccga
     2041 atgtggtgtc ttctccgact ttgtctgccc aaatctgaat ggtgccattt tccaatatcg
     2101 taccggcaaa gacttcactg ccttctgttt ttgtgacagg aacggattct cctgtgatac
     2161 tggcttcgtt gatatgccct tcacccgtaa ggactgtacc atctactgga actttcgcac
     2221 cagttttgac aagcagaaca tctccttcat ctacatcatc tacatccact tcttcaaatt
     2281 ctccgtcttc tgtttggacc aaggcacttt ctggtgccat gtcggttaat tctttgattg
     2341 ctgaacgtgt ctggttcaat gtacgctgtt ccaaataatg tccgaacaag aacaagaacg
     2401 taacgatagc cgattcttca taattttgaa taaaaagcgc accaatcgct gcaatactga
     2461 ctaagacatc aatactgatg actttgactt tcattgcttg aaacgcttga atggcaattg
     2521 gcgtaatccc aaagacagag gcgatgatca aagaccactc tgaaagtcct acgttttcta
     2581 aaacaaagtg actgaagaaa ccgagtacga ttaacaatcc actgatgacg gtgataacat
     2641 ttttcttgct taatatatat tgttgcatgc tttttccctc caaatttaat ttctctttta
     2701 ttgataaaca cagtataaga taattgccta agtaattaat tgactgacat caagtttaga
     2761 aagttctctt ttatcgtttc taagcacctc gaatcggatt gcaacaaaaa aagcaatcct
     2821 ggaaaaatag cttttcttcc agaattgcct agttcattta atctgttaag cggccttgac
     2881 ttttgcttta aggaccggat agcctacgtt ttcgatagct ttttgaatct cttcaatcga
     2941 agtgacagct gaatcaaaat tggctttgac tttgctggag ttgaataata ctttaatgct
     3001 gtctttatca acaccgttaa ctgattttac ggcaccttca attttttgca tacaacttgg
     3061 acaagacaac gtttctaatt gcaatatagc tttttccata attcctatct cctttatgtg
     3121 tttttatttt atcggcagca gattaatgaa cactgccttt tcctttttac aactgtagta
     3181 tagctcactt ttagcaaacg aaaattgaca catattaagt tttgcacttc tttttatctt
     3241 tttgctcaat tcaccatctt atacaaattg agttaaattg tattcgtttt tcctcctata
     3301 ggcgtatact aaagaaaaca cttgaggagt ggggaacatg gaaacagaac gccaaatccg
     3361 tgaattggaa aaagcgatga atacgataga caaccgccgg ttgtacgagc gtttcctggc
     3421 agtcaagttg gttcttgaag gccacacccg gaaagaaacc gggcacacca tcggccgcga
     3481 cgagcatact gtcagccact atgtgaaaaa ctactgccat aatggaatcc aaggtttagt
     3541 ctccaaaaaa caaagcggac gaccttcacg gctgacagat gaacaggaag cggaactggt
     3601 ccaaatcgtc gcctaccaca ctccggagga agtaggtttc aaatcccgtg ccaattggac
     3661 attggcgatt gtgtgtgacc tgattctccg ggaatggagt tatggatata cgcaaaaagg
     3721 cgtggcggac ctccttcacc ggctaggact aagctggacc cgtccgacgt ataccctcaa
     3781 aaaagcgggt tccaagaaac aacaggtatt tttggaagaa accttcccga gcctaaaaaa
     3841 aaaattgctg aatagcgata ttgaccacct cctattcgag gacgaaacca tgatccggga
     3901 ttaccaagcc attggctgca cctggtttct ccgcggccac cagcgaaaaa tccccactta
     3961 tggacaacac aaaggggtaa aattgatagg caccttggat tatgaaaccg gtcggatcat
     4021 ttgtacagaa gaaggggcat acgacgcagc ggccttcctc cgttttctgg agcggacgtt
     4081 gcgggaatac cccaccggaa aaatcgtgat ggtattggat aatgccctta tccaccacgc
     4141 caagctgatc cagtcttttc ttgaagaaaa cgctaggcgc ctggcactca tcttcctccc
     4201 tccctacaga cctcagttga acctaatgga aggcgtttgg aagtggctca aggaatcggt
     4261 catcaacaat gttttttttg accacgtcca gaaaatcaaa cagtcggtcc gcggtttctt
     4321 agccgatgta aacgagcgtc cccttgaggt gattgaccga ctgtgcgtgc ggatgtaaag
     4381 tatttaattc aacctatata gaaagaagtc ttgtacttca ttttagtcta atgacagtca
     4441 taacatcatc gacagccccg ttattgagtc aattctattc aataatttct tctacttgta
     4501 ataacgttgt attcaacgct taaaatattc cagcattaaa caccagttcc acactgttct
     4561 ttttcaatag atacaccgat tctttgcgag ataattggta tttatcagga gatttaggac
     4621 ggttttctac gttcattttc ccacctcttg aatttttcct tctattatat aggtatatag
     4681 ggcgttattc tgtcatagat ccaaactctt tactgcggta tctttgtctt cctgggtgat
     4741 cccaatatag cgtaaggttt cccgttgtga agaatggttt agtagtgact gaatgatctc
     4801 tgttttggtt ccttgcttgt acaaaaaata gccaaaggtc ttacgcatcg aatggggacc
     4861 aatttctttc aacccaacca ccgttcctgc ttcatgaatg atccgccaaa ggctttgtcg
     4921 ggtcaaaggc gtatctgctc cttttcgact tggaaacaaa tacttgctga agtcttgtac
     4981 ttgcagctgc tcaagaaatt ctgattgaac ataatcaaga atcacttttc gtaatttgtt
     5041 cgaaatgaaa accgaattgt gtttagctgt tttttgttcc ttcatggcca gacgttcttt
     5101 gacgatcacc cggccacggg aaatttctaa cacgtcactt aatttcaact gtcttaaatc
     5161 ggagattcga tacgccgtat tgatcccgat cgaaaaaagc accaaatttc gttgcccata
     5221 ttttccggaa gccaaaattg ccttcatggc gtcgatcttt tttttatcgc gaatcggttc
     5281 cacgctcttc atttttagtc ccctctcaaa tgttacttaa tatgattata acagataata
     5341 agtcacattt aaacaagcta tagaaaaaag gccctaaaag aaaagtgatt tcctcttaga
     5401 gcctctaagg atggagcgat tttgatgaat gtgacagaat ccgattgtgt cacattaggc
     5461 ttttgatatt ttcggtaaga tttgattatc tcagaacatt aatattgccc ctttaaaaaa
     5521 ttttgtcagt acttttaagt tattctattc tgaaaattca aaattttcag tctatataat
     5581 taacttatat tcagttttac caaatgataa gctttaaaaa gtattcagaa tttagtcatg
     5641 ttatggatcc aataaagtag atatggaata tttattttct cttgtctaat ttataagaat
     5701 atgatttgtc aaagaattaa agctatgaat tattca
//
LOCUS       DAGCWU010000154         5677 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.156, whole genome shotgun sequence.
ACCESSION   DAGCWU010000154 DAGCWU010000000
VERSION     DAGCWU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.156"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            321..1244
                     /gene="truB"
                     /locus_tag="MBZ71_002371"
     CDS             321..1244
                     /gene="truB"
                     /locus_tag="MBZ71_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBT4430281.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1248..2189
                     /gene="ribF"
                     /locus_tag="MBZ71_002372"
     CDS             1248..2189
                     /gene="ribF"
                     /locus_tag="MBZ71_002372"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBT4430282.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2584..3369
                     /locus_tag="MBZ71_002373"
     CDS             2584..3369
                     /locus_tag="MBZ71_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4430283.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3382..4233
                     /locus_tag="MBZ71_002374"
     CDS             3382..4233
                     /locus_tag="MBZ71_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBT4430284.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4357..4809)
                     /locus_tag="MBZ71_002375"
     CDS             complement(4357..4809)
                     /locus_tag="MBZ71_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4430285.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5274..5642)
                     /locus_tag="MBZ71_002376"
     CDS             complement(5274..5642)
                     /locus_tag="MBZ71_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBT4430286.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         1902 a          922 c         1155 g         1698 t
ORIGIN      
        1 actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca gtcttttttt
       61 tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa atccaatcaa
      121 atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca taacatgatt
      181 tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa aaaccgttca
      241 ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca gttaaatcaa
      301 aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa cgcggaatga
      361 caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa atcggacatg
      421 ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga aaagccacaa
      481 aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata actctaggtt
      541 tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca gtagatatgt
      601 ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag atcacccaga
      661 taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa tatgcccgca
      721 ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt atccggacaa
      781 gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa gtagaatgcg
      841 ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt ggttaccctg
      901 cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa gatgctctga
      961 cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt ttgctgccaa
     1021 tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta tggcagaaag
     1081 taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg ccgtcagaag
     1141 aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac ccgaaagaaa
     1201 aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg aatatcatca
     1261 agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt ttagtactag
     1321 gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt atggaaattg
     1381 cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca tcgattgtct
     1441 ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa aaagaagaaa
     1501 aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct tcttttgctc
     1561 atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca aaatatgctg
     1621 tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct cattttcctg
     1681 aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa caaggtgaaa
     1741 aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa gaagcagcaa
     1801 aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat gcacgaggca
     1861 gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat ttgcctaaag
     1921 aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc atgggttcaa
     1981 ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg tatattttgg
     2041 actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa tttttaagag
     2101 atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag gatgaacaag
     2161 caacagctga atatttttct cagcaataaa acattatgca taaaatctct tgtttatgta
     2221 taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc tgtgacaaaa
     2281 gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt cttcggaaat
     2341 aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct tatttcttga
     2401 agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     2461 tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt gtacactcac
     2521 tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     2581 actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     2641 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     2701 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     2761 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     2821 ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga aaatcagcat
     2881 ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa tttgactggc
     2941 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa aaaaggaaat
     3001 atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc tcattacgct
     3061 gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta cgcaccaaag
     3121 ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa tgcggcaaaa
     3181 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     3241 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     3301 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     3361 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttgggtgg
     3421 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     3481 actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc tccagctggt
     3541 atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt catcggacaa
     3601 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     3661 gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga atggtctgga
     3721 agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg ttctgcttta
     3781 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat cagtctatta
     3841 gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt tgatgcaagc
     3901 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     3961 attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac aatagttgga
     4021 gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     4081 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     4141 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     4201 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tctcatttat
     4261 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     4321 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg aaatatctgt
     4381 ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat tgaagttacc
     4441 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     4501 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt gattgattgc
     4561 tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     4621 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     4681 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     4741 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     4801 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     4861 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     4921 ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     4981 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     5041 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt ggaaaattca
     5101 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     5161 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     5221 tataagctga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     5281 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     5341 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     5401 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     5461 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     5521 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     5581 ttgatctttt gttgatgttc tttttctttg atctgattga tgatcggctg aaatttttcc
     5641 attctaaaac gcctccatta agtggtttga tttattt
//
LOCUS       DAGCWU010000155         5586 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.157, whole genome shotgun sequence.
ACCESSION   DAGCWU010000155 DAGCWU010000000
VERSION     DAGCWU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.157"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..242)
                     /locus_tag="MBZ71_002377"
     CDS             complement(<1..242)
                     /locus_tag="MBZ71_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430287.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRK"
     gene            complement(229..756)
                     /locus_tag="MBZ71_002378"
     CDS             complement(229..756)
                     /locus_tag="MBZ71_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430288.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(779..2116)
                     /locus_tag="MBZ71_002379"
     CDS             complement(779..2116)
                     /locus_tag="MBZ71_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBT4430289.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            2236..2715
                     /locus_tag="MBZ71_002380"
     CDS             2236..2715
                     /locus_tag="MBZ71_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430290.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(2830..3062)
                     /locus_tag="MBZ71_002381"
                     /pseudo
     CDS             complement(2830..3062)
                     /locus_tag="MBZ71_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3065..3382)
                     /locus_tag="MBZ71_002382"
     CDS             complement(3065..3382)
                     /locus_tag="MBZ71_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430291.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(3493..3675)
                     /locus_tag="MBZ71_002383"
     CDS             complement(3493..3675)
                     /locus_tag="MBZ71_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430292.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            complement(3712..4512)
                     /locus_tag="MBZ71_002384"
     CDS             complement(3712..4512)
                     /locus_tag="MBZ71_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430293.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(4649..5068)
                     /locus_tag="MBZ71_002385"
                     /pseudo
     CDS             complement(4649..5068)
                     /locus_tag="MBZ71_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5064..5372)
                     /locus_tag="MBZ71_002386"
     CDS             complement(5064..5372)
                     /locus_tag="MBZ71_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430294.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
BASE COUNT         1680 a         1196 c          693 g         2017 t
ORIGIN      
        1 aatttcctac tttgttcctt aatttcagca tttcccaatt ctttgtattg agcttcctgc
       61 atagctttca ctttctggtt attcttgact acttcgattt gtttactttt aaggtcttcc
      121 aattctttga cattattatc ataagcattt ttcttttgaa atagtaccac acaacaaagg
      181 atcacaatta tggaaagtat tccgatcgcc tttttagcag tagtgatttc atgattttgc
      241 ataaaaagca cccccagtca ctttcttatc attatttagt gtgatggtta atgtggtatc
      301 atacactttt tttccattgt attgaacttc aaaatcggtc aatgcttcat acaacccact
      361 actagatgtc ttaattccaa agtttgttag cttaaaagta aaaggcttat agtactcgat
      421 tttttctttc tcactactct tatcagtaat tggtggttta cctgttacaa taaatagttt
      481 tctcaaaaat tcttcacctg attgatctgc aaacttattt tgaactgatc cattcaacat
      541 ttcttgaata tcattttcta aatctttccg taatccatcc acctcatttg gtttaacctc
      601 tttaaagaat gtcagcttat cttcaatatg cacactgtgt ttttcagctt ctatgtcctg
      661 attctgctta ttgaagaata ggattccaac aagcagtgct aataacatac cagaaagcaa
      721 agaaatgata attgtatctt tcactttact attcatctat atcgtccttc attccaattt
      781 atttttcgat ttcaaattta gcttttccta caccgctaaa gaaatgcaca ttatctgatc
      841 cgtccactcc atcaatcaac gttaccgaaa ctccccttgt tggagcttgg gttggtggaa
      901 cattaaagtt agcacataga aatgatccat cttcaaacac cgctataact acaccagtat
      961 gccctgcatc tccaacagcc cataaccatc catccgaagc actaaaccca taacctacga
     1021 ttggtttgtt cgttattttt gctcctgccg atttgtagct ttcccataca taaccaccat
     1081 ttccagtagt cggttgattc ccatcgtata attggctcat ataagcccac gtcaactctg
     1141 tacactgacc ttgatatatt ccgttaaaag gtgaagttga ccaatctggt tctttgaatt
     1201 ttggtagcgt gatcgctttc ccatattttt tcaaagcttc ttcataagtc atattcgcta
     1261 ttacttgacc ttttttaccg ttaatttttc cttctttccc aattgatcca gaaccagaat
     1321 tagcgtcatc cgtagttgca caggcatctt ctactgttcc tgatgctgaa cttgatgaat
     1381 tattcgatcc atctccggta cttgaacctc cggatatttt attgatctta ctttcatctt
     1441 ttgcaccgtc catacccttg cttttcatgt acttccagat ttcttttgca ttttcaccac
     1501 gtacagctat tacagctgat tcggaatcag ctgaaatttc ccagttttta tgaaaagtta
     1561 ctgcattttc tacgacatca tcagaagcat ttaaagcata atcttttaat attcctacga
     1621 atcccccatc acctttgaac ataaagtcca attgggcttc tgtagtccac caagaaccgc
     1681 cattattttt cttagaccaa tcaactaatg ctgtatgtcg cccagctgtc cattgaccat
     1741 agccaatacc catcagacca tcagtaaatt gtttgatgaa gtcaacttct ctctgtccat
     1801 ctccgtaata cccttctact gctcgaggat caatacctga ttcttgtttc cagttaccta
     1861 aaatacctgc aatactagca cctgataagc catattctcc attcatgtat ttgtaaatat
     1921 cttcaatatt tttggctaca tcacctgtta cagcgccact tccagtatcg tttccatcgc
     1981 tatctgttcc tgacccacta ccttcagtag tacagttaaa ttgtgcatca taagtagcta
     2041 tcccatcagc atgtatcact tgcggattta ttgtgattac actcattgca atccctgtaa
     2101 attgcaccat taacataagc aatgtggcaa tctttctctt agattttttc aataactacg
     2161 cccccaaaaa gtgtactttt ttatgttatt atgataatat aataacataa attaatagaa
     2221 aggaaaagct aataaatgac tgataaaaca agtgatttac ctaaactagc tccaaaaaca
     2281 tctcgagtaa agagcaaaaa agatggaaaa acaaaaaatg ctgtaaagaa aaagtcagta
     2341 aaagaaacat cctcgttact acttgatatt ccaatgagtt cttttgatga caatacggac
     2401 gaaacttctg ttaaaattgc aactcacgtt ggaatgaaca atcaaaatga ttctttttac
     2461 aacttgttaa acagcctaag agagttggaa gataaaaaaa atcaggtaat cgcatcaaaa
     2521 gctcctgatc tcacaaccga tttggatgct gaaattatcc aaatcaaaca agatgagcga
     2581 tcagttaaat cgctactgaa aacctacttt gatggttttg ataaagaact agctccttat
     2641 agccaagcaa tcctaactaa accaatacaa gctccacata tttcaatttc cctacaaaaa
     2701 aagagtgatg attaaacaat cattactctt ttttagattt ctggtaagcg ctgatcgcaa
     2761 aaaccatcaa caatattgat tcaaaaaata tgttcattcg tgtctctttt ttccattata
     2821 gatttcccac tattccatta aatcgaaagt cacaccgatt actacaaaga ttaacgtgac
     2881 cacattcaaa aacattaaaa gatatgctga taatctcatc atttttttag ttcacttact
     2941 ggtaatctaa acatttgaac gattgcactg gaaataccat aattagcaat gacaaacaaa
     3001 aaatacgcaa taatttcact tacacctaga tagatcagta aaataaataa gtcacttaac
     3061 attgttagtt tcctcccagt atatgatttt catttttggg aagtctttta taatccaatt
     3121 cctcaaaaaa aggaaccata gtgtcactaa tataggtttt agtttcaggt accgccaaaa
     3181 ttccccaatc aagaatttct tcccatgata catcatcaaa ttctaaaata ttatgatcaa
     3241 ctgacctatt accaagttca tatccaccaa agatcaccat attatcaatc atgtatagaa
     3301 tttcatcagg cgaaccatga gttaatgttt cgtcctcttc aagcagttcg tttaatcttt
     3361 gtttgtattc ctctcttttc atacttttat cccttctgac tcattttttt gaattttaac
     3421 atttttaacc caagcatgac tatctttaca tgctattaat ttttatctcc attttgatat
     3481 ttctgttttt ttctactcac caatcaaccg taaatcctca atattctcac cttcaagcga
     3541 atatgaatat acttcgccgt aaatattttc tataactacg acttcttctt caaagtctat
     3601 ggactgcaca tcaaaccatt ctcctccgcc aaattcgtct atgattagta cctgcaatat
     3661 tttatttact gccatttcaa tttcccctta taccattcta tgtcttccaa actatttttc
     3721 ttctttccat accaaggttt cacgtatctc aaaattttct ttaaataagt gttccggtac
     3781 ttttataatt aactcttttc ctacttttat cagatagtct ttttcaccta atgagatttg
     3841 accagtcggt aactgtatag aaactctttt agttttatta aattttttag ttttcaatac
     3901 agtatacggt ctacagaatt tctctaagcg ttcaaatctt ttactatcgt attggaaata
     3961 agctttagga acagataaag tacaaaaact atcggtaaca gcacttatgt cttttttaga
     4021 aattatccca actttagaac ttctaaggaa agttgctaat ttcttatatc caccatgact
     4081 tgtttcaata tagatgctat tgtattggct gtcgtctatc tcatttattg tttcaacctt
     4141 tttcctgaca ggtgtttcaa cgagttctcg aacctcttct tcactgtacg ctaatacaat
     4201 taactcttcc ccatttacca tctttagaac atacgcttgc ttcttttttc ttggcttatt
     4261 ttgagattca acataaccag acatggaatc tttatacttc caagcatgta cttttttctc
     4321 taatttctgt atctcttttt tcagctgtag atattcagac attttttcat tgtaacgttc
     4381 gcaatataaa caatattcat cttttccaaa atcatgatca agtccttctt caatatgttt
     4441 attcaacaaa ccggaaaaat actgatcata ctgcttcttt cgtaacttca gctctcgtaa
     4501 atcttcagac atttcatttt tcctttccaa ttattggtgc catttcacaa tttgggattg
     4561 actaaacacc ataattaatt tgtaagctaa cacaattaca actgttggta attgtgttag
     4621 tctccatcaa cttgtctggt ggcttttttt attgcacaaa tttcatagct ctcaaaaagc
     4681 gcttggcttg aaaaggtgtt attctacgaa ccttattcgc caatttccaa tctgataaag
     4741 caatttgcca ttcgtgcatt tcaaaatccg gcatgatttc tttgatatac tgtagctctt
     4801 tcttttctct ttcttttaag aaacggctca tcgagtttaa cattggaact aaggatgcgt
     4861 aggtacttga ataacttcca atgcgtttcg tagtaattac ataatcaata aaaacactat
     4921 taagaaaggt caagtttata aactctgatt catacactct aaaatttgca gttaatcgct
     4981 tcgccttgtc accccaatag tcacttttgt tgtaggcttt ttcccctcga atatagcact
     5041 ctgtttctct ttcgtaataa tagtcatact tttttcatca tcaaatctga gagtttttga
     5101 ttatcttttt cactgtaaaa taatctactc gttaaacttt taggataatc tttgatagaa
     5161 tatgcatccc aaacaattct ttgaacatat tcacgatcta tattttctct gacttcccca
     5221 gtccaaaaat gttgaccata atggcatctt tctattacat tgttagcaga aacttcaaaa
     5281 taggattgtt ccctttgtat cctcatacca atcatcatag cgcaggaaat aactattaat
     5341 aacaatcctg tttttaatct attattccac atgcagtttc cacatcctta tcttgataaa
     5401 aatcctcttg accctctcca attactgttt taaatataac caccaaaaca agatcatgct
     5461 aagaaatcca agagtatata agatgataca ttctttgtta attatccaaa aaactctgat
     5521 ttctttgagc ttttcacgca ttaggaaaca ccttgttata tcttttttgt atttctttca
     5581 attgtt
//
LOCUS       DAGCWU010000156         5508 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.158, whole genome shotgun sequence.
ACCESSION   DAGCWU010000156 DAGCWU010000000
VERSION     DAGCWU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.158"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(47..268)
                     /locus_tag="MBZ71_002387"
     CDS             complement(47..268)
                     /locus_tag="MBZ71_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430295.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(265..555)
                     /locus_tag="MBZ71_002388"
     CDS             complement(265..555)
                     /locus_tag="MBZ71_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430296.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(552..1736)
                     /locus_tag="MBZ71_002389"
     CDS             complement(552..1736)
                     /locus_tag="MBZ71_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4430297.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1918..3321)
                     /locus_tag="MBZ71_002390"
     CDS             complement(1918..3321)
                     /locus_tag="MBZ71_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBT4430298.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3343..4116)
                     /locus_tag="MBZ71_002391"
     CDS             complement(3343..4116)
                     /locus_tag="MBZ71_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430299.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4126..4503)
                     /locus_tag="MBZ71_002392"
     CDS             complement(4126..4503)
                     /locus_tag="MBZ71_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4430300.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4524..4838)
                     /locus_tag="MBZ71_002393"
     CDS             complement(4524..4838)
                     /locus_tag="MBZ71_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4430301.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5038..>5508)
                     /locus_tag="MBZ71_002394"
     CDS             complement(5038..>5508)
                     /locus_tag="MBZ71_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBT4430302.1"
                     /translation="GDDNGVDLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDR
                     IKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNV
                     SDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
BASE COUNT         1630 a         1087 c          936 g         1855 t
ORIGIN      
        1 ttcataacaa aatatatact tcctatttta tcatgaaaaa tatctgttaa gcaccaatga
       61 ttcggttaaa taagtttaat aacacatctt tcacgccagc tgcattgaac acaagtccga
      121 cggcgattag agcaataatc aagaagccaa tgagttttga aaattctcgt ttaaaaccta
      181 aaaacagtcc aatgacaaca atcgctaaca ataccaatga ctgtgcatta tccaagaacc
      241 agttgtacaa attctgccca aaattcatgt gattgtcctc ctttccttaa gatatttttc
      301 tcgataatag atatagcttt ttacacgcct taataattga aaaggggtta aatgctctcc
      361 atagacggtt actcgattgg tataattatt ataaaatctc accttgtaaa aatcataggt
      421 atagatggta tcaccagctt gccgttttcc ctcaataatg agagacgtat atttccctgt
      481 attttgtata tacgcttgaa actcaatgcc acactgaaac cctaacttct ttaattcttt
      541 tgttaaaagg ctcatgtaat caaatcctcc atggctagat gttgttgttc aattaatttc
      601 tcatgttttt ctgtcagttt ggcttcctga atcatggtgg agataatggt tgtaccgttg
      661 agaacatcta aaatgcttgc gatttttaat gtaggggcga cttgatgatg taaccagttc
      721 aatgttcgtt caaaagaata aggctctggc tgtgtggtta ggcgtaatgc tccacgattt
      781 ttaccgataa accattccca acgttcatta gttttccaat cactccgacg tttagtgtcg
      841 tctttatctg caaagcggat atagcgattg ataatttcaa aggctgtttt ttccgcctgt
      901 tgatgtgcca ataaatcttt catggcttgg gtggctcggt cattttttaa ccgaatttca
      961 aaccgattct ttacttctgc gtcttctaag ggaacatcat ttttcacgta ttgctcataa
     1021 tctttctcat atatacagaa atagacttca ctttttaatg agccaatata aagtgtgttt
     1081 cccatgtatg gtttttcatc acggtgaacc aattcaccac tgcgataatt tttaaagctt
     1141 cgaaagacgg aaatgcattc ttcctgttta catttctttg ccaaatctgg aatattcaaa
     1201 atccctgttt tatcgttgat agctaaatcc aagcgtttga aaataccttt atgttccatg
     1261 caatcttgaa agaaatcata ccagctccga tgttgagcca atagaaagtt ttcaaattgc
     1321 cgacagcctt ttcctttcag ttctaataaa acacctttac ttacatcatg agagactagg
     1381 acaacaatat caccgaaacg atagtgttcg ggataagaat aatagccaaa gtcttcgtgt
     1441 agcatataat ccatattcaa acgtaaaacc tcaccgataa tttgagccac atcattggta
     1501 gggaatcgaa tccgtacata gtcgaataac atctccaatg ggttatcggg attgaagcgt
     1561 tccaaagcgt tcagtaaatc ttctttcacc tgttgagtag ggactgcctt gcctgtttca
     1621 atatccgata ggtaaggtct ggtaatatga gaagcaaccg ccagtttatt ttgagaaacg
     1681 ccataagcca gtcgcttttc tttcaattcc ttaatccaaa ttttttcgtt ttccattcgc
     1741 aaacctccaa tctaaaaaag atgtaaacta tgacccttga gtttacacct ttcatatttt
     1801 ccgcaatcct ttgtaccatc aacattggtt gatgagaatt gcctgttttc agttgtattt
     1861 gtgcccccct gttagataga gggggctaaa ggattggcgt ggctaacgcc acaccaatca
     1921 aagcaagtct gaacctgtac ctttcacttc gtgcggtaca ggctcagatt gctttttttg
     1981 aatgactttt cctatttctc ctaagaaatc gtaccttttt ggtacaaggg gtgtataaaa
     2041 ctcactaatc acactttttc ctgtatccac gtaacctctt cccttgatgg gttttaagaa
     2101 aaattgtttt tgtgtatcgc ttccgaacat cataccaaag cccatttcag acatacgacc
     2161 caaagccaca cggaagttaa agttgtcccg aatcccatca ctaaaatatt ttgcgtctgg
     2221 acgttgacag gcaagaatta aaaagaagcc tgcttgtcgt cctaacatta caatttgttt
     2281 gagtttatga ataacggtgg tactttcacg acctagcata tccatcattg ccacatattc
     2341 atcaaaaata agaaaattgg cagacaaacc aagataagca taattttctc ctgttttata
     2401 gttagggtgt tgtttcatgg tttcactgcg atcaatcata tcttcgtaaa attgattgat
     2461 acatgccaac atatcttctt tacggtaata gacattattc atgaccgccc ctaagtctgc
     2521 caaatcagca tttttagggt caagaatata aagctttgca tttgtatgca ggagagattc
     2581 aatcaatgtg agaataaaat aggattttcc actaccagtg cccccagcaa ttaacatgtg
     2641 agggagagaa tcgaactccc aatagaccga ttccatcaat ttgacacgtc catcttgagc
     2701 taccacgtct tcaatgccaa ttcgattgcc aatagtatca taaagtagga catactctac
     2761 ataagaatct ttcaacgttt tttccactaa ttcacaatac aatcctgttt ctagtttgtt
     2821 ttctaggtgc aaaagctgat cttgatattt ccctaaggtg atttctacat gaatatggat
     2881 tagtccatct ttcattcgat aatatatttt aggaaaatgg cggattcgtt gtttggtttt
     2941 cttgctgggt aaatctttaa agaatccctc tgattgagtt tcttcggctt cataccaatg
     3001 attttctaaa atcatacgag ctaacttttg acggtgctct aactgtttta tagaatctat
     3061 acgatagtga taataaagcc agctgataag tccgcataca aaaagagcag tgagaatact
     3121 gctccaaaag tatggcgtat gaagttgata gaaaaatgta tctaactgaa ctgcttgcca
     3181 gttcaccgat tgtaacgctc tccaatgaaa agggagtagg acgactagaa aaactaccag
     3241 caatgaaatt agtacaaaat gatagaccaa gtgcttatcg ttggctttga ttcgctctcc
     3301 tctgtcatga agcaatggca taaaaatcct cctttccacc tatcaaaata attgtgtatt
     3361 gtagatacga gcctgtttat caaaagttgg ttgagaaatg gcttcatatt tctgataagc
     3421 gattcgcttt tcaaagcgta aatttttttc tttcggataa taatgacgtc gatagagagt
     3481 gtgtcctaac agttttttcg cttcttttgt caacggatag agatagcgaa acatgcgtcc
     3541 attgattttc tcaatccctt tgtattcgca aaatgcctgg gaaagccaat gctttttctc
     3601 tacctgttca aagcgagcat tttcctctaa taaaaggcga gcacttcgag ggtgtatctt
     3661 ttcccatgac tgcttatcac gatagacact tgtcttgaaa tacccgcagt aaaagaagtt
     3721 ggacgcttga tagacatagc cacattttcc ctcaattcca tctgccagtg tatataaaaa
     3781 atgacagtcc gtatgttctt tcaaccactt aattagagct gaaagagctt gactgccaaa
     3841 ataattggtt tgattcattt ctggaagaaa acacatcttt ccaatttcta aataatctgt
     3901 ggtttgaagg gaactgtcgg gaaagagttt gcgtatggtt tgtaggggtt gcgtacccca
     3961 gcctaattcc acaacgccta ataatttttc ttctgaaaaa atccctagaa agtatttaca
     4021 caatctaggg agtatctttg aataatggta ttgccgaata aaagaaatgg cttcatcctt
     4081 cggaatttct ttcaaaatca atggtgtgtt tgtcataagc aacacctatt tttcactttc
     4141 ggacgcttct tttttatatt tctgatgagg ttttgtctgt ccttttaata caagatcgtc
     4201 tgctttcacg taccaatcaa cgtctgcccc tcgaaaagta gccgtcgcta cggtatctgc
     4261 cacaggattg attaattcca cttctgcgtt gtagtcaaat tctttcaaag gaacagaagc
     4321 ggggatactg acttgtatca tgcgtccttg tccttttgat tttaagtcat aggtacgttc
     4381 tttaatttct tcggaaaccg taccatcttc attctggata cgaacctcac gacgcaaggc
     4441 ggaaaatttc aatgtgccaa atgtctggtc tttttcaatc acaatacctt ctgctaatct
     4501 catatttttt cacctttcct ttcttatgct ttcaccatat cgtctgcgtg cataatgtaa
     4561 ttggtaaatc ctcgattgcc aattttataa ccttctgcgg taatatgtgg gttgatgagt
     4621 ttgacttttt cgtcggattc aaaatgtttc tcgccagctt cggagggaag aatgacaaca
     4681 atatcatctg ctcgttgaat cgttgagtat aaattgtaac tacgagagag aatggttggt
     4741 ctgccattta ttcgacgttg ttcaatgttc ccttcaccag catattctaa ttgtccaaat
     4801 gttttttcca tattgggaat gacatattta agttccatat ttttacctat cctttctgtt
     4861 tttatgtttg aatgaatctt atttgtgtga tggttttaaa ggggggagag cgaccttttg
     4921 attcttgatt ttttattttc ataagttcac ttcctttcaa aattgggtaa aaaaatagac
     4981 gctccgtact gagcgtccga ttcctactat atttaaaaca acgactacct aggtatttta
     5041 aatattataa tcaaattcta ttttatctaa taattgagtg ggataaagca agtctccttt
     5101 actcgtaaaa taacccacct ctgagaaaag aaataaatta ttttttgctc ttgaacaaat
     5161 tacgtatagt agttttctcg ctgaactaat tcctttatca gaaacattaa aaatttcatt
     5221 ccaatgagga attttcccgt ttagaagatt aatagcgata acagtatcat actcatttcc
     5281 tttaacttta tgtgctgtag taagtgtgat accatctcga gatttaaaca tcttcttaaa
     5341 actagataca ctactagata atgaattatt ttttatccga tcttctactt cgttaataaa
     5401 aaatagatag tgcttaaaaa tagacgggta atttttctcg tcaatattaa gattaacaaa
     5461 tatatgtttg gtaacaaatt tgtataaatc aacaccgttg tcgtctcc
//
LOCUS       DAGCWU010000157         5416 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.159, whole genome shotgun sequence.
ACCESSION   DAGCWU010000157 DAGCWU010000000
VERSION     DAGCWU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.159"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            98..2425
                     /locus_tag="MBZ71_002395"
     CDS             98..2425
                     /locus_tag="MBZ71_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4430303.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2551..2880
                     /locus_tag="MBZ71_002396"
     CDS             2551..2880
                     /locus_tag="MBZ71_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBT4430304.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2920..3924
                     /locus_tag="MBZ71_002397"
     CDS             2920..3924
                     /locus_tag="MBZ71_002397"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBT4430305.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3921..5350
                     /locus_tag="MBZ71_002398"
                     /pseudo
     CDS             3921..5350
                     /locus_tag="MBZ71_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         1930 a         1034 c         1065 g         1387 t
ORIGIN      
        1 aaaagcccca aactttctat acatcaataa aataataaaa attaaattta gaataacgtg
       61 taaaggatta tttgatgatt ttttattgag aggagggatg aaaatgatta agaaaactca
      121 aaataaaatc caacgtatat gtcaaaaaca ttttgtttct agactcaaag ctgtatctaa
      181 aagaaaatac ttacatccca ttatgatttc gatcatcttg atttttactt tcttagggag
      241 cttatcaagt atttatgtaa acgtttatac agaaacgtca aattttgcta accaaacgac
      301 agaggagaca aatagtccac cctattctga aatacagtcc atagaaagca ccaatcagtc
      361 tagcaacaac gagacaacct caaccacatc ctcgactcaa ctgattgatc cgacagaaaa
      421 agaaatcaca gcagcaaaag cagcagggga gaaagcttat tcagaaacag gtagaagcca
      481 gacatttagt actgttgcgg ccactgcgaa tttcaacgga cataccatac cagatggata
      541 tacagtgata tatgccaaag atggagtcat tgttgcggtt aacggatcga tgagtgcgga
      601 taaaactgcg ttacaaggcg taaccaaagc aatcgctttt attgataacc gtccagatat
      661 ccataatctg tttattggct ccacagtaaa tggtactact atcacattcc caaaaagtat
      721 ctatatgggg gtggctaccg ctggaaatac gatcgattca gttacaccca atgcggataa
      781 aagttattct tttacaccct caacatgggg agggcttcct gccggaggag gtatgagtac
      841 tttttcaatc ggtatacagt tagcaaatga tattccattc acggggtact cgatcaaaaa
      901 tgggcagatc gttgatggta agggaaatgt ttcaaatttt ccaacctatc aagcagcaac
      961 ggttcaggtc aatgcgaatc ggacaggaac tttttcttat acagtcaatg gtggagcagc
     1021 aaaaatcgga acgtacggga ctacattatc tggaatccat gtgggtgaca aagtggttat
     1081 cactccaaca gcagtaacgg gctattcttt tgcacaaaca aacggttctt tcacagccct
     1141 agaaagtaac gtcgatacgg tgacctatac aggcaaacaa acaccaatca ctattaagta
     1201 tcaagactat tttggacaga tgattgctcc ttctaaaata atgaatctta cttacggaag
     1261 tgctgcacaa gacctgacga ctaatacgcc tactatcaac ggatatacct ttacagctgt
     1321 ttcagcgaca gaaacaaaga accaatcagc aatgagtgtt ttagctagtt tagatacaaa
     1381 tggaaatgta atcgtaaagg acgcaaatgg aaaacagatt tctgaagtca tcctatatta
     1441 taagacaaat gcaacagtat caatcaatgc aaatggcagt aagtactatg atggtctttc
     1501 tgttgtaccg atcgttaaat atgcctttaa tgataaaaca gaatcaacat cacttttaaa
     1561 caatgatctc agtattgatc aaatcacatg gaatccaact gatttcaaag cgatgaatga
     1621 aaagggagtt gaagtaactg cgcctactga aattggaacc tatacgtctt ggcaattgac
     1681 aaaaagcgga ctagctaaac tagctgccag aacaaactat ttatttacta tcgtgcagac
     1741 atctgatgtc tatacgatta agcaaatcag cggtgcggta gagctcagtg gcagtaagac
     1801 atatgatgga aaagcaggtg tcccgtcaat ccatgtcaag ctagcagagg gtgtcactag
     1861 ttcgcttact ccagtacaag tggcacttag cagtatggat tacacagcag atgcacaatc
     1921 agcaaaaaat atggttaatg ctggcagcta tacaattaaa ctaacgaata gcggaataga
     1981 caaagtcaaa gcagcaaatc ccaagttgtc atttactgat atagccaaca catcaggaac
     2041 ttataccatc tataaagcta acgcagtgat tactgtcgat gacgctgcct ttaattatga
     2101 tgcccaaagt cactcgattt cggttggaaa tctccacgtt accggtgttg tcttaggcga
     2161 atcattggat tataccctaa cagttaacag ccggacagat attggatcac aaatagtaac
     2221 gattagttta ggcgaaggaa gtgtaaacaa taattatacg attatcgcaa cagataccgc
     2281 taagttgacg atcaatccga ctctcttgct tccttcgaca ggtgggatag gtctactgcc
     2341 ctttgtcttt ttaggactga tctttatttt ttctggcttc ttttatttta tccatcgtaa
     2401 gaaaaaagca ggtgaacagc gatgaaaaaa acaaaaaatg tacgtgtgga tatatttgct
     2461 gttctctttt ttatcctact ggtctttttt ataggttctc cgactgtgtc agctagagaa
     2521 tcacctatta catttacttt gcataatcga atgttcgaaa gcgataagat gccaagtatc
     2581 agtcaaaacg acggaataaa tatcccggat aactttcaag gagtaaaagg gttgaacggc
     2641 gttactttcg ctatatatga tgtctcggat gaattttaca agctgcggtc agaaggaagt
     2701 agtgtggaag acgcacaaag aaaactagca caaaaaagtg atagtgaaaa aatactggca
     2761 gaaaatgtga caaaaacagt agacgaggaa gagggcattg cttcttttag tgcatcggac
     2821 aaagatgagc aaggtcgtga cgctgtttac cgatttgcgg aaataaagac ttcagattaa
     2881 gtaaaagaaa aatcagcacc atttgtcgtt gtgcttcctg tgacagattc atctaatcat
     2941 aaattgacga ccatccactt gtatccaaag agcgaacaaa aaatacctgc tgcactcaca
     3001 ttaacaaaaa cagtagagaa taagcagaca gattttgcag atggggataa agttccttat
     3061 ctcatcaccg taacaattcc tgagaacatc aacgagatag gaacctatac gatcaaagat
     3121 acagctgatc ctcagttatg gctagaacca gaaacgatcg attttttgat tggtggagac
     3181 aagattcata catttcacac acaaaaaaca aaacatggtt ttgtcttatc agattctgga
     3241 aaagatctta gttcgtttgc tgggaaaaaa ttaacgatcc gttaccaaat gacattgaaa
     3301 gaaaacagtg aaaaaacgac atttgataac gatgtacgtt tgacgactga caatcaaaca
     3361 gtagaaacac atcttgccat ccaaacagga ggcaaacaat ttgtcaaagt ggatcggcaa
     3421 acgaaacaaa aactagcaga tgctcaattt ctagtcaaaa aaaatgacca atacttagcg
     3481 caagaaaacg ggataaatca ttgggtagcc aaagatgatt caagagcaac gatttttact
     3541 tccagtaaag atggaacatt ttctgtccat ggattgtctt ttggaagtta cgaactagtg
     3601 gagattaaac cgcctaaagg atatatattg agtcaatcaa ccattctctt tgaagttgaa
     3661 aaaggcagtt atgatccgct tcatcctata ccattggaaa tcatcaacga accaaaagca
     3721 aatccgacga ataatggaaa aacgccagtt cagatgcaga caaatggtcc atcaaatgaa
     3781 aaaaatggag gaagctttcc aaaaacaagc gaagagatgc ttggaggttt ctcaatttta
     3841 gggttgttgc tagtcctttc aacagggaca gcttggtttt ataaaaaaca acaaaaagga
     3901 aacaatagga gggaaataaa atgaaaaaca tcaaaacaaa aatatggacg tttttaggaa
     3961 ctgctatcat gctgcttcct ttcgttctcg gcctaggaac tgcagaggtt tctaggccgt
     4021 atcacctaca ccagaaaatg ttacggttaa tttgcataaa ttgaaattca cttcagcacc
     4081 agaaaatcaa ataaacaacg gaacggaatt gacatttccg aatagcgaac cgttgaacgg
     4141 cgttgaattc aacgtatatg acatcactgc tacgtactat ccaagtaaag acacagcagt
     4201 tccagcagac gctacaccct tcgcaagtgt aacgacttct ggtgaaggat tagctaacct
     4261 gactcttcca ggtaaatctg atgggaaaga tgctgtatac gtctttgtag aaacaccaaa
     4321 gcccggtgtc gaaacgtcgc caaatatcgt gttaagctta cccttttata acccgaattc
     4381 atcagaccct gacaaacctt tagacacgat tcacctatat gctaaaaatg tcatcaaaga
     4441 aagcaatatc aaaatccaga aagctgggaa cttttcaggg gtagataagt tagcaggggc
     4501 gcaattccag atcaaaaatt cagagaatca attcgtgacc ggttttgatg aaaacactgg
     4561 tgtcgctctc tatgatcctg ctgagtcagc gttgacattc acaactgata ctgaaggatc
     4621 catcagtgtc aatggattaa tagacggcaa ttatacttta gttgaaacaa aagcaccaga
     4681 tggttatcaa ttaaccaatc cacaaaaaga aactactttt atagtaaaaa acggaaaatt
     4741 gcaacaaaca accggtatca tccatggcgc agatgaagcc aatcttggga atccaaacgg
     4801 tgtagtcaat cgggaaaata caactacgga taacacgatt actgtagaaa atgtccaaaa
     4861 ctacggtgat ttccacttca tcaaacaaga tgtgaacacg caagaaaaat tagccgatgc
     4921 agaattttct attctaagag caaaagatcc aactgttaaa ttaatgaaaa aaatagcaaa
     4981 tcttgcctca ggagaatacc catatgcttg gtcagatgaa gtcacaggtt ctgactggga
     5041 agaagtgaaa ttgatttctg attcagaagg gaaattttca atcgaagggc tgaaacaagg
     5101 ggcatactgc cttcaagaaa caaaagcacc agaaggctac gcattaccta agaatgaagc
     5161 tgcgtacact gaatttacag tagatgatga tccaacaact tctgatgaaa caacgatcga
     5221 taaccaacct aaaggaatct tgccttcaac tggtggtatg gggatcatcg tattcatctt
     5281 ggtaggtact gctttagttg gaggagcagt gatctacttc aaaaaacgcc atcaagaaac
     5341 agaagcttaa actattggaa acaaaaatca tacgataaat aaacgatagt gattcaaatg
     5401 gggagaaagg ttgtga
//
LOCUS       DAGCWU010000158         5356 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.160, whole genome shotgun sequence.
ACCESSION   DAGCWU010000158 DAGCWU010000000
VERSION     DAGCWU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.160"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            7..78
                     /locus_tag="MBZ71_002399"
     tRNA            7..78
                     /locus_tag="MBZ71_002399"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Gln,seq:ttg)
     gene            96..180
                     /locus_tag="MBZ71_002400"
     tRNA            96..180
                     /locus_tag="MBZ71_002400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:Leu,seq:caa)
     gene            558..1310
                     /locus_tag="MBZ71_002401"
     CDS             558..1310
                     /locus_tag="MBZ71_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4430306.1"
                     /translation="MGKGIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPI
                     EFMDFAEEVIELITARISSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLY
                     PNEYDIAIEVVERINHRYQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTIL
                     SHLEKSYSNVYDKESLTYHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCT
                     ALKIKDIIEQQFQLAIQENEIIYLAIHLARIY"
     gene            1414..2178
                     /locus_tag="MBZ71_002402"
     CDS             1414..2178
                     /locus_tag="MBZ71_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBT4430307.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2165..3607
                     /locus_tag="MBZ71_002403"
     CDS             2165..3607
                     /locus_tag="MBZ71_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4430308.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3618..4948
                     /locus_tag="MBZ71_002404"
                     /pseudo
     CDS             3618..4948
                     /locus_tag="MBZ71_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4961..>5356
                     /locus_tag="MBZ71_002405"
     CDS             4961..>5356
                     /locus_tag="MBZ71_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4430309.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIF"
BASE COUNT         1782 a          812 c         1138 g         1624 t
ORIGIN      
        1 tattcttggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
       61 cgaatccagc taccccagtt attattttta aatttgccgg cgtggcggaa ttggcagacg
      121 cgctggactc aaaatccagt gtccgttgag gacgtgccgg ttcgaccccg gccgccggta
      181 taattatcaa tatagcaact ttcttataga gagttgctat attttttttg tttttatgta
      241 attacctcca aatcaattcg atacaagtat tatcataaaa cgagtaaaaa agatattgat
      301 aacgctttta ttatgtgtta aactattttt gtaaattatg tagggtgcta ctattcaatt
      361 aggcacgacc taagaaaggg ttctttctta ggtcgttttt tgtctttaaa aattgtttgg
      421 agacaaaaat atgtatgaag gagtctgcaa gttgttttgt tttatcatat agtggaggtg
      481 aattaacggt gtatgtgatg aaaaagctga atcataatgc tgtattgatc gatgacgatg
      541 gaatagaata aattgcgatg gggaaaggga ttggcttttc tgctaaagtg agtaagccat
      601 ttgatgttga ggatgctgac aaattatttg tattagattc taaagaaaag atcaaacgat
      661 tcagtgagat agtctcgcag attccaatcg aatttatgga ttttgcggaa gaagtgattg
      721 aactgataac agctaggatc tcctctccat tagattcaaa tatctatatt acattgacag
      781 atcatattta ttttgctatt caacgaaaaa gagaagatag agatgtcaca gcaattatgt
      841 tgccagaaat gagattcctt tatccaaatg aatatgatat agcaatagaa gtggtcgagc
      901 gaatcaatca tcgatatcaa accgagttag ggtcaaatga agtaggattc atcactatgc
      961 atatcattaa tgctgagcta ggagagccta atagtttaaa gagcttaaaa atagtagaaa
     1021 tgacgaccac gattttgtcc catctagaaa aatcctattc aaacgtttat gataaagaat
     1081 cactgactta tcatcgctta atggttcata tcaaattcct ggttaaacgc ttgatttatc
     1141 aggaagaaac gccagtagaa aatattggtt tttttactga aacatttaaa catagcaaag
     1201 cgtattgtac agcactaaag attaaagata tcatcgaaca gcaatttcaa cttgccattc
     1261 aagaaaatga aatcatctat ttggcaattc atctagcacg aatctattaa aataagggcg
     1321 taactatcta taggcgcaac caaatgacac atagtaaatg gactatgatt atttggttgc
     1381 gcctatttta gtagaaaaaa gaaggagaat gaaatgggaa aagtgatttt taattcggat
     1441 gattttggct atagccatgg tgtgaattat gggattatgg atgcttatca gcgaggaatt
     1501 ttgacatcaa ctacattgat ggcaaatatg ccgggtttcg agcacgctgt gaaactaaga
     1561 aaggaaatgc ctaggctagg tgtaggagtc catttaacat tgacgtgtgg caaaccactt
     1621 ctcaaaactg tcgatactct tatcgaaagc ggagagttca gatccttatc ttattatcag
     1681 catccattta acattgacaa agatcaactg tatcaggaat ggaatgctca gattcaaaaa
     1741 atttatcatg cggggatcat accaactcat ttagacagtc atcatcatac tcatactttg
     1801 gatgaaaacc aggaggtagt tgttacatta gcaaaaaaat atgatttgcc ggttcggagc
     1861 aactttgaac gaaaagatga agttcgtcat gtcgattatt ttgagccttg tttcgatgtc
     1921 gtaggagaaa ctgatgaaaa aaaacgacaa atcaatacga gtgtggaaga atatttggca
     1981 agtttactca tgatattgag agaagataaa acgatcgaag tcatgtgtca cgcagcgtat
     2041 ttggatcagg aattgttaaa tggttcaagt tttgtgtatc ctcgtactca ccaagtcgaa
     2101 tttttaattc attcagcatt tgctcaaaaa gtgaaaaatg atcctttgat tgagttagta
     2161 acatatgagg atatttaata gaggaggaat aaaaatgaat gttaaatcaa atgtgcagaa
     2221 gcttggaaga gcgatgctac ttccagttgc agcgatgcca ttagcgggat tgatcatgcg
     2281 tttatcagct gatgatatgc tgaacatccc agtaattggt gcagctggaa atgccgtttt
     2341 tggaaacttg gatatcttat ttgctatagg agttgcaatc ggttttgtca aaaataagga
     2401 caaaggaata cctgcactta ctggtttttt agcgattgct accttgaaaa aaggcttaga
     2461 aatcatggac ccagaagtga atatggggat ttttggtgga attatttccg gtttagtagc
     2521 ggcatggacg tataatcgct ttaaagaaca aaaattacca gtagtttttt cttattttgc
     2581 gggcgaaaaa ttcccattga ctatggtgat gattctacaa acaatcgcag ctattttttt
     2641 gggctttatc tggccgctca tccaaattgg gattgataat tttgcaaaac tgctcgttga
     2701 tatgggggca ttaggtgtgg ggatatttat gtttttgaat cgtttgctga tcccctttgg
     2761 actgcaccat gtgttgaata cgtatattta ttacgattta ggaagctata catctcctag
     2821 caaggaagtt ttcagaggag agatgacacg ctttatcaac ggagatcctc aggctggact
     2881 gtttttatca ggtttttttg tagtaatgat gtttggtata ccagctatat gtctagctat
     2941 ctataaagca gcttataaag aaaataaaga gatggtcaaa ggaatcatgg gaagcggtgc
     3001 aggaacttca tttattgcaa atatcactga gcctgttgaa tttagtttca tgtttctttc
     3061 tcctatgttg tacgtgatcc atgcactttt tgccggttta gcagggattg tctgttactt
     3121 attcaatata cgaattggtt ttacattcgg agcatgtatt gttgattatc tcatcaattt
     3181 tcgaatcgct accaatgcta ttttgattct tccaatcgga attttctttt ttgccttgta
     3241 ttacgttact ttttattatt tgatcaataa gagaaacatt cagacattag gacgtgaagc
     3301 taaggcagag tttggcaatg aagtaacctt agaagaaaca gagttagggt tagcaagtaa
     3361 aaattatgaa tatatggcaa caaaaatgct acaagcgttt ggaggaaaag ccaatatcct
     3421 ggatgtctat agttgtaata cacgcctacg ggtagaggta gtggatccca ccatggtcga
     3481 ggaacagcga atcaagcaat tagggataag cgggatcatt aaaccaactg aaaaaaatta
     3541 tcaaattatc atcggcttag aagtgactta tgttatggca gaattcaata aattattaga
     3601 agaatagaaa ggaaaagatg atgatgaaaa aacagattat cactatagct ggaggcggga
     3661 gtacgtatac accaggaatc gttcaagcca ttttgaacaa tgaaaaacgg ttgccattat
     3721 ctgaaatacg tttatatgat attgatgaag aaagaaatat ggatatgtac ttgattgtta
     3781 aatttatgct gaaaaaaaag gtttttcaaa tattcgcatt cgtgcaacgg atgatccaaa
     3841 gcttgctttt acaggatgcg attttgtttt ctcacaaatt cgtgttggag gattggagat
     3901 gcgagaaaag gacgaaaaga ttccacttaa acatggatta gtaggacagg aaacgtgtgg
     3961 actcggagga tttgcttatg ggatgcgctc aatcaaaggt ttattggaaa ttgtcgatca
     4021 tatacaagac tatgccccca aagcatggat tttgaattat acaaatccag agtcgatcgt
     4081 ttctgaagct gttcgtcgta aatatccgaa tgtaaaaatg attaatgcct gtgatatgac
     4141 tatttcaatt gaagaaacga ttgcagtgaa ttacggttat gatcgtaaga attggatcgt
     4201 tacgtattat ggtttaaacc actttggctg gtatacatcg atttatgata aagaattgca
     4261 acgagaaatt atgccagaga ttattgaaaa actaataaca aaagaaatgc aagtcgccga
     4321 ttttaatata ggagacaaaa cttggcaaaa aacctttcaa atgatgtcag tcatcacgaa
     4381 aaattttcca agtaatatcc cgaataatta cttagagtat tacttatatc ctgatatggt
     4441 cgttgagcat actgacaaag agtatacacg tgctaatatg gtcatggatg gacgagagaa
     4501 aaataccaaa gaaatggctg ataaaatacg ccgcggtatt gtggaagaag tattgaactt
     4561 taattttggg gagcatggac aatatatcgt cgatatagct atttccttgt tgaatgatga
     4621 tcgtcgtcgc tttatgctaa tcgttcctaa tcaaggggcg attccgaatc tacggtcaga
     4681 tgcagttgtt gaaataccgg catatgttgg tgcaactggt gtggagacaa ttacactaag
     4741 aaaaccaatc agtgattttc ataaaggatt gatggaggca caagttgcag cagagaagtt
     4801 actagtagat gcttactttg aaggttctta tcaaaaagct ttacaagcat ttacattgaa
     4861 tcaaaccgta ccaaatgcca gagtcgcaaa aaaaatacta gatgaaatga tcaaagtaaa
     4921 caaaaaatat tggccagcat taaagtagag gtgataaaaa gtgcatttct ttaaaacaag
     4981 aaacattcta aaagctgtag ctagtggtca aattattcca ttaacagcag taaaagacga
     5041 ggtcttttct tcaaaaatga taggagttgg atacgctata acgaatcatg atggatatat
     5101 ccatgcgcca gttaaaggaa taatcgataa tatatttcct acccgacatg ccataacgat
     5161 cgaaagtgaa tctggcagaa aaatactgat ccatatgggt ttagatacag ttgaactcga
     5221 aggaagtccg tttcagatac gggttgaaag taaccaggag gtaaaagaag ggacactgtt
     5281 agctattata gatttgaaga agttggagca agcacaaaaa gatacggtga tcattgtagt
     5341 ttctccaggc attttt
//
LOCUS       DAGCWU010000159         5354 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.161, whole genome shotgun sequence.
ACCESSION   DAGCWU010000159 DAGCWU010000000
VERSION     DAGCWU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.161"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            16..1005
                     /locus_tag="MBZ71_002406"
     CDS             16..1005
                     /locus_tag="MBZ71_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4430310.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            987..1448
                     /locus_tag="MBZ71_002407"
     CDS             987..1448
                     /locus_tag="MBZ71_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBT4430311.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1498..1962
                     /locus_tag="MBZ71_002408"
     CDS             1498..1962
                     /locus_tag="MBZ71_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4430312.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1976..2725
                     /locus_tag="MBZ71_002409"
     CDS             1976..2725
                     /locus_tag="MBZ71_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4430313.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2725..3555
                     /locus_tag="MBZ71_002410"
     CDS             2725..3555
                     /locus_tag="MBZ71_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4430314.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3555..4202
                     /locus_tag="MBZ71_002411"
     CDS             3555..4202
                     /locus_tag="MBZ71_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4430315.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4300..5076)
                     /locus_tag="MBZ71_002412"
     CDS             complement(4300..5076)
                     /locus_tag="MBZ71_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4430316.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5158..5301
                     /locus_tag="MBZ71_002413"
                     /pseudo
     CDS             5158..5301
                     /locus_tag="MBZ71_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1826 a          734 c          962 g         1832 t
ORIGIN      
        1 ataataccta ttagagtgag ggataaagaa atgagacaac tacacaaaga acagattgaa
       61 acagcaatgg cagcgttaaa ggaaaagaca attgatcatg tatattttac agcatgtggg
      121 ggttcacaag ccgttttaat ggctggtcaa tatctatttg ataaagaatt aactgtacct
      181 tgtcatgttt atactgctaa cgaatttaac tatgctatac caaaaggatt atctgaaaat
      241 tctttagtta tcacttgttc ccattctggc acaacaccag aaactgtaga agcaacaaaa
      301 aaagcaagag aatttggagc aatgactatt tgtttatcta atgaagaagg atctcctcta
      361 tgggaagcag ctgaatatcc tgttcactat gattggggta aagatgttga tgctagtgat
      421 ttaaataaag gaattcttta tggtttatta tttaatattt tacttgtttt agatggctct
      481 aaaaaatggg aaacttgctt gaatgaattt gataagttaa atgatcttgt tattgctgaa
      541 aagagcaaat ttgaaataac tgctaaagaa tggggtaaaa accaaaaacg agataaaatt
      601 atttacacaa ttggtagcgg tattaattac ggagaaattt actccacagc agcttgctgg
      661 ttcatggaaa tgcaatggat taattctaat gctattcatt ctggggaatt tttccatggt
      721 ccttttgaaa tcactgatta tgatgtacca ttcatcttag tgaaaagtat tggagataca
      781 agatttttag atcaacgggt agaagacttc actaaaaaat tcagtaataa attaatggtt
      841 ttagaccaag ctgattttga cttagaatct atagatagtg tagcgaaaga atatattgca
      901 gctgtactta gtggcgttgt tattcgccaa atggtagaag cgattgcttt tgaaagaggc
      961 cattccttag atgttagacg ctatatgtgg caaatgaaat attaattaat aggaggttgc
     1021 aaaagatggc aaatcaatta tttcacattg tgattacaac acattctacc ctttgtgagg
     1081 gttatttaaa ggcaacaaaa ttaattttaa tgaatgacgc cacaggtatt agtgctgttc
     1141 catttaaaga aaatactagt ccagatgatt ttgaggagag tatgaaagaa ataattagtc
     1201 agcatcattt gcaacctcta attattttaa cggatttaat tggaggaaca cctaataatg
     1261 tttctattaa acagttgaac aacaatagaa ttcaaattat tgctgggatt aatttacctc
     1321 ttttactgga gttattaatg gcccagtcag ctggtaaatc tatagaagaa atagatttta
     1381 aggaattact gaataactct caaaaaagta tggttcatat taattcgctt ttaaaagatg
     1441 tcagttaagg tggcaatttc tattctataa attttaaaaa ttgaaggagg aaatagaatg
     1501 attaaattag tgcgtgtaga ccacagatta cttcatggac aagtaatatt ttcttggact
     1561 aaacagtatg atattaacca tattattgtg gctgataaca aagttccagg agatccaatg
     1621 tctgtgatgg ctttgacaat tgctaaacct gctgattgca aattagacat tgttaaaatt
     1681 agtgatgtta aagggattat ggaaacatcc ccaaatgata attacatgat tttaattaaa
     1741 ggacctaagg aagcttttga attagttcaa aatttaccag aaattaaaga aattaatttt
     1801 ggcggtgtgt ctaagaaaag cgagtccaaa caatatggaa aggcagtctt tttaactcct
     1861 gaagaattag aagccacaaa aaaaattctt gatcaagaag tgaaaatatt tgtgcaacaa
     1921 gtcccatctt caagtattga aaatgtagat tttagtaagt aaggggggaa aataaatgtt
     1981 attaaaagcc attttattag gtttagttgg tgtggtagct gttattgact ccaggttaat
     2041 tggtcgacaa aatattggta gaccactaat tttgagtaca cttgccggac ttgtgctagg
     2101 tgacttgcat acagggattg ttcttggggc atctttagaa ttaatttcta tgggattcgt
     2161 ctcaattggg gcggctggac ctcctaatat gcaacttggt agtattattg ccactgcttt
     2221 tgcaatttta actaatagta gtacagaaac agccttaaca atagctatcc cagtggctgt
     2281 agctggtgaa tttatgagta ttatcatgcg tatgataatt gctcaatttg gacatgctgc
     2341 agataaagcc attgaaaata ataaatttag aaaagctcaa attattcata tttattggtc
     2401 atttattttt aatgcttttg tttactttat tccaatcttt ttaacagtat attttggtgc
     2461 tgacgttgtg gcagatttag tagataaaat tccagaaatg attacagacg ccttgactgt
     2521 ctctggaaat atgctttccg cattagggtt tgcaatgcta ttaagcacaa tgttgagtaa
     2581 aaaattatat ccttacttta tttttggatt ctttattgtt gcctattcag gtctttcact
     2641 aattggcgtg acaatttttg cagctctgat tgcctttgta atggatcaag ttaaatatgg
     2701 aaaaagagaa gaggcgcatg gttaatggaa aatagtacag aaaaaaaacg tttaatcaca
     2761 aaaaaagatc ttcataagat gtgtcttcgt tcatggacaa tcatgtgttc gtggaattat
     2821 gaaagacaaa tgcatatggg atttatgtat gggatggcgc caattttaaa taaagtttat
     2881 aaaaatgatg aagagaaaaa aagagaagct tatactcgtc atatggcatt ttttaactgt
     2941 acgcctcaaa tgactccatt tattatggga ctttgtgctt ctatggaaga acaaaatgct
     3001 caagcagaag aaggaaactt taatactgaa tctatctcca tgattaaaac cagtttaatg
     3061 ggtccttttg ctggaattgg tgattcattt ttccaaggta ctttaagaat tattacattt
     3121 gggattggat tgtcttttgc taagcaaggt agtattttag gaccgatttt agctgttggc
     3181 ctttttgcta taccttctat tttagctgtt ttttacggcg gtaaattagg atatgagtct
     3241 ggcaataaat accttactaa attgtatcaa gaaggtctta tggatcgagt aatgcaattt
     3301 gcttctatca ttggtttggc tgtagtaggt ggaatggtgg ctagcatggt gccaattgtc
     3361 acccctttaa aattcactac agggggaaca gaacttgttt tacaagactt acttgattcc
     3421 attattcctg gattattacc acttgtatgt actttgggaa tatataaatt gattaagaaa
     3481 aatgttagta caaatatgct cttattaggt tgtattttgg cagggtttgt tttaagtata
     3541 ataggtatct tataatgatt aaggctctaa tttttgatat ggacggagtt ttaattgata
     3601 gcgagattat gtatcaaaag tgggtattta attttttaaa acaagaaaat ttaagctatc
     3661 ctttgtattc ttatcaaaaa aagattggaa ctacagcaag tatttttgat gatatagaaa
     3721 agtacaatga gggatgtaac agtttagaat tgaagggaaa atttactaaa tattggaaat
     3781 cgcaaaagat tgattacttg gatatttttc ctaatatact aattgaacag cttgaatggt
     3841 taaaaaagaa aaaaataaaa atggcaattg cttcttccag tccaatggat actataaatg
     3901 aagttgttac cacttgtaaa atagagtcat attttgattg tttaattagc ggaagagatt
     3961 tgccagaaag taaacctaat cctactatat tccttaaagc ttcagaacag ctcagaattc
     4021 cagttgaaga atgtctggtg atagaagatt cttataatgg tataaaagct ggtaaaagag
     4081 caaatatgaa ggttcttgca attaaagata aacgtttttc tcaagatgta agtctagcag
     4141 atgatgttat ttacgatatt aaatatctgc gacaagaagt acaggtgaaa tttggaatat
     4201 aaataagttt tttgatgtat ttagagaata ttagtaaaaa taaaaatcat gcccaaatct
     4261 aatcaaaatt gattgctttg ggtatgattt cttttaatat caatctatta ctttaggatc
     4321 tgaatcattt ttatgcaccg tatctaaagt gatattttct gtgacttcaa taacataact
     4381 aattctatca caacgaactg tggattgaga ataatgaaat ggttgattat ttttatcttt
     4441 gccaaccttt tctaaaataa atagcggatc tcctactata cattgcaata agtcggaaag
     4501 ctcaggtgtt gcagataaac cgtttatttc cataatagag ctcgtagctt tgacatcata
     4561 gtcatcttct agaagtttat ataatgaagt atattggcta cctttttcaa ataaagttgg
     4621 aaatttttct tctgatgcga aaaaattatc aattcccatc ggggcaccgt cttcccacat
     4681 taaacgttta atattgtaaa ctctatcagt tacattgatt tgtaatttat tagctaaaaa
     4741 ctcatcagcc tttataattt tagaatgtaa aacagtatat ctaattttat gtcctcgggt
     4801 agaaagagaa tcgctaaatc catgaactcc agctaatttt aaactttctt ttttttgagt
     4861 tacaaaggtc cctttccccc ttaatcgttc aactgttcca ctttctgtta aaagtttaaa
     4921 agcttttcga atggtaatct tacttactcc ataaatttta cataattctt cttctgtagg
     4981 taattgctct cctggaagat attttttttc acgaatatcg ttacgtatag aagcttctac
     5041 ttgtagatat aatggtctat tatcttgaaa attcatagta cactccctaa aaagttatac
     5101 ttcattatat cacaatttat taaaataaaa gattaagata taaatataaa tgaactagtc
     5161 tttgactaac caatgcccgc ttttgaaaga aaacaaattc attctgtttt aagtaaaaat
     5221 ccttatgtta ccactcattc tgaaggggat gagccttatc gctacttggt tgtggaacca
     5281 agcgataaga caattttata aattcaatat taattaaaat aaaaacaaac aaagtagatt
     5341 attttacttt gttt
//
LOCUS       DAGCWU010000160         5320 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.162, whole genome shotgun sequence.
ACCESSION   DAGCWU010000160 DAGCWU010000000
VERSION     DAGCWU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.162"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            84..818
                     /locus_tag="MBZ71_002414"
     CDS             84..818
                     /locus_tag="MBZ71_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4430317.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            834..2003
                     /locus_tag="MBZ71_002415"
     CDS             834..2003
                     /locus_tag="MBZ71_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4430318.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2019..3014
                     /gene="lacD"
                     /locus_tag="MBZ71_002416"
     CDS             2019..3014
                     /gene="lacD"
                     /locus_tag="MBZ71_002416"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBT4430319.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3093..4019
                     /locus_tag="MBZ71_002417"
     CDS             3093..4019
                     /locus_tag="MBZ71_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBT4430320.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4038..5288
                     /locus_tag="MBZ71_002418"
     CDS             4038..5288
                     /locus_tag="MBZ71_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4430321.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1743 a          884 c         1102 g         1591 t
ORIGIN      
        1 aaaagattgt tcttagtatt gactcataaa aaataccact actatactta cattgtggtt
       61 atagccagtt aggagagaca tcgatgcaaa atacctttca atcaaaatca ctttattatc
      121 agctggtaga aatattgaaa caacgaatcg aaaacagtat ggtcgctcat gacaagctgc
      181 catcagaaag agagttgacc agtttttatg gcgttagtcg cacgacggtg cgactagcat
      241 tgcaagaact ggaaaaaatg ggctacattt atcgacaaca tggaaaagga acctttgttt
      301 cggaattaag gacaaatgcg actgatctag ctggcgcata tagttttacc gaacaaatgc
      361 gggcaatggg caaacatcct gaaaccaaga tattggcctt caagaagatt gaagcaacta
      421 aatatttagc gagtcatctg aatgttcaac taggtgagcc tttatttcaa ttagaccgat
      481 tgcgcttagc tgatgatttg ccaatgatga tcgagaaatc ctatttgcca gtaaagctat
      541 ttatgtccct ttctcaagaa attctatttc tgaaaccttt atataatttg ttttcagaga
      601 atttcaatca aatcgtacgg ttggctgacg aggaatttta tgccagcatt gtccagaaag
      661 aagatagtga gttgctaaat attccagaag gatcaccagt gctccattta gttcgctcaa
      721 cctataatct cgagaacgaa gtgattgaat ttacattaag tgtagcgcgg gctgataaat
      781 ttcattataa gatcaggcat ctgcgttcca ctaactgaat tggaggaaag aaaatgttta
      841 tgttctcaaa agaagaacta cgaaagctag gagcagaaaa tactacccga gaaattcgac
      901 agcaaccgga actgtggcat gagacgtttg accgttatca agaaacagtt gcagatttag
      961 atcgtttgtt tacggatgcg aaacaacatg ctggagagcg accaatccga gtcattttta
     1021 caggggccgg aacctcgcaa tatgtgggag atacactaat tccgtttctt actagtcaag
     1081 gaaatacgaa ccagtttatt tttcaaagta ttggttcgac cgatatcgtt gctacgccaa
     1141 aagaatacct gattcctgat gagacaacga tcttggtctc atttgcccgt agtggaaata
     1201 gtccagaaag tctagccgca gttaagatgg ccaatcagct ggtcaacaag atctatcata
     1261 tcgtgatcac ctgtgcacca gacggattat tggctacaca aatgcctaca aaaaaagaaa
     1321 atctagtttt actaatgcca aacaaagcca atgacgctgg ttttgctatg acaagcagtt
     1381 tcagctgtat gacgctgact gcgctactta tttttgatca agcaagcaat cagcaaaaag
     1441 aaagatatgt tcatcaatta agccgactag gagaagaagt catccaacga gaatcagaga
     1501 tccaagggta tctggcatca gactttcatc gcatcattta cttaggttcg ggaagcttag
     1561 gcggattgac tagagaagcg caattaaaag ttctcgagtt aactgctgga aaaatcgcaa
     1621 ccgtatttga tacatcttta ggattcagac atggtcccaa atcttttgtc aatgagcaaa
     1681 cattggtttt cctgtttgtc agcaatgact catatacgca gaaatacgat gtagacatgc
     1741 tggaagaaat caaaaatgac ggtattgccc aacgcgttgt tggaattggt caaaagggtt
     1801 cattgtttag tggtgattcc tttgaattag ttgcagattc gttactgccg gatggctatc
     1861 ttgctttagc agatatcatg gtagcccaaa cgatcgcatt actttgttcg gtgaaggttg
     1921 gtaatacacc agatacacca tcgccaagtg gaacagtcaa tcgtgtggtc aaaggcgtaa
     1981 cgatttatcc atatgaaaaa taggaaagag tgtgaagcat gttagaatgg tcggaaaata
     2041 aaagacaagc aatggaacgt ctatcgacgc cagaaggcat tattaccgct ttggcgatcg
     2101 atcagcgagg cgcgctgaag aaaatgatgt cggctttagg gatggaagcg acaaaggaaa
     2161 aaattgagca gtttaaaaca ctaatttccc aagaattgac tccttatgcc tcagcaattt
     2221 tattggatcc tgaatacgga ctgcctgctg cacagaagcg tgcagcagcg agcggtctgc
     2281 tgttggcata tgaaaaaact ggttatgatc aaaccgtacc cggccgcttg ccggatctgc
     2341 tgtcggactg ttcagttctg cgattgaaag agttaggtgc ggatgcggtg aaatttttac
     2401 tgtattacga ttatgaagaa gcagaggaaa tcaatcggca aaagcaagtt tttgttgaac
     2461 gaattggaag cgaatgcatg gctgaaggac tgccatttta tttagagtta ttgtcatatg
     2521 atgctaaaaa tgtagatacg aaatcgcttt cctatgccaa agcaaaaccg cgcaaagtga
     2581 tcgaaatgat gaaagaattc tcgaaaaccc agtataaagt agatgtctta aaggttgaag
     2641 taccagtaaa catgtcattt gttgaaggaa atgcaacagg ggagatcgcc tatacacgtc
     2701 aagaagctgc cgcttatttt aaagaacaaa gcgatgcaac agatttgccg tatatcttct
     2761 taagtgctgg ggtatcagcg gcattattca tggagacatt gcgttttgcc aaacatgctg
     2821 gggcacgatt caacggtgta ctctgtggca gagcaacttg gaaagatgca gtcgctcctt
     2881 tcgttgaaaa aggagaagcg gcaactttag aatggctgac agaacaaggg acacaaaaca
     2941 ttcgtcaatt gaatgaagtg ataagagaaa ctgccatccc atggtatgag aaagtaagtg
     3001 aaagcacttg ttaatgtttc taatatatta gtagtgataa gagatatgtg tgattataat
     3061 ttatcattat tgaaactggg aggatatttt atatgaataa aagattgtta gattattcag
     3121 cttcagattt tttgaaagct aagccgatgg aattaaagca ggcaattttg gcttctgaag
     3181 gtagaacaat tatggcagaa aatgtgccaa gtagctgtcc ttacatggga aaatcagtga
     3241 caaatgcaga attggagaga gctgctggag ctgatttaat tttgtttaat gcactagatc
     3301 tttttaatcc tcaaatttca ggtgtacctt taactctaaa agagaatcca ataatctgga
     3361 ttaaaaaagc tttaggaaga gctattggtg taaatttaga acctgtagac aaagaagttt
     3421 tgatgagtga aaatcgaact gaaatttcaa gtggtagaat cgtttctcct gagactttaa
     3481 aaaaggcaaa tgaattaggc tttaatttta tttgtttaac tggaaatcca ggaacaggag
     3541 tatctaatag aactattgag aaagcaataa cattatccaa atcaattttt aatggattaa
     3601 ttatcgctgg aaaaatgcat ggtgctggtg ttgatgaacc aattatgaac ttagatatag
     3661 caaaaagatt tattaaaaca ggtgtagata ttttattagt tcctgctcca tacactgttc
     3721 cgtatttcat ggaaagtgat ttgcgaggaa tcgttgatta tataaaagat tttaatagaa
     3781 ataaatctat tgaggaaaaa gttctagtac taacggctaa tggaacaagc caagactctt
     3841 ctgaccaaga aactattaaa aaaattgcat tagcatcaaa agcatgtgga gctgatattc
     3901 aacacatagg ggattcttta aatggtattt gtcttccaga aaatattttt gctttgggtc
     3961 aagcaattcg gggatatcgt catcaaatta ttatgttagg aaaatctaat tatcgttaat
     4021 taaggaggaa atgaaagatg atcgacaaag ttcaaaaaat agctgcaaaa attagtaatc
     4081 aaagacatct actagcttta agagacagca tggctgcagt tatgccatta atcattattg
     4141 gttccttttt tatgttatta gcaaatttcc cgatagaagg atttacaaaa tggttatctg
     4201 aaattggagt acaatccttt ttgaacaaag catcagattc aacatttggt attattggat
     4261 tagctgttac ctactctttg ggacacaatc tagcaaagac ttatggtgta gactcattat
     4321 cagcaggatt attatctttg agtggatatg ttttacttac acctttattg gatgcagatg
     4381 gttctatagg atttccaaca aaatactttg gtacctcggg tctttttgtt ggtattattg
     4441 tagcacttgt atctacagaa attttccggt ggtttgtact taaaaatatt acaattaaaa
     4501 tgccggcaag cgttccacca aatgtaagcc gagctttcgt tgctattatt cctggctttt
     4561 ttgtcgttct actatggttt attgttgttg taatttgtta taaacttgga attgaaaatg
     4621 ttcatgctct tattgcagat acactcgcaa aaccactatc tttactgacg aaaactttac
     4681 caggaattat attggttata cttattcaat gtttcttttg gatgtttggt attcatggtg
     4741 cacaggtaac ggggccaata attgaaccgt tattattgca aaactctgat gtaaatagaa
     4801 ttgcttatca agctggaaaa gaacttccta acattataac ttatgaattc ttatataact
     4861 ttgttttttc tggtggtgca ggttgtttat ttgcacttgc cattttaaca tttttctttt
     4921 caaagagtca ggaaaataaa actttaggta agttatcaat cgctccagta agttttcaag
     4981 tagcagagcc ccttctattt gggtttccaa ctatattaaa tataaaaatg ttaattccat
     5041 ttgtaactgc accagtagta acaacattaa ttacttattt ttcaatgagt atgggattag
     5101 ttgcaaaacc tgtaggagca acaatcccat ggacaactcc accgataatt gccggatttt
     5161 tagcttctgg tggacgaata agtggtgcag tgattcaagt aataacaatt gcaataaatg
     5221 ttttgatata ctatccattc tttaaattag atgataatgc caagctaaaa agcgaaaaaa
     5281 atgattagaa ctacctatat gtttctaact gtagttaagt
//
LOCUS       DAGCWU010000161         5205 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.163, whole genome shotgun sequence.
ACCESSION   DAGCWU010000161 DAGCWU010000000
VERSION     DAGCWU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.163"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            28..2253
                     /locus_tag="MBZ71_002419"
     CDS             28..2253
                     /locus_tag="MBZ71_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBT4430322.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            2271..4154
                     /locus_tag="MBZ71_002420"
     CDS             2271..4154
                     /locus_tag="MBZ71_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBT4430323.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            4135..5109
                     /locus_tag="MBZ71_002421"
     CDS             4135..5109
                     /locus_tag="MBZ71_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBT4430324.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         1707 a          929 c         1154 g         1415 t
ORIGIN      
        1 ggtttccgtt ttgttgggag tgaaatgatg gatattacgc atagaaaaac agataaaaat
       61 ttcatcttat ttgatgaaaa gagccgagtc ttccatttac gaaatacatt tctttcttat
      121 ctgataaaga tagaagaaag caatgtattg gcgcatattt attttggcaa accagtcaag
      181 cagtacaaag acaataaaaa ttatccccga agagatcgtg gattttcagg aaatgttccc
      241 ttaaatcctg accggtctct gtcgaaagat acactaccgc aagaatactc ttctcatggc
      301 agcatggact ttcgaacgcc cgcatcgatc attcagcgca agaatggatc agacttactg
      361 gatctgagat atgacagcca ttatattaca gatgggaagc cagagatcga aggcttgcct
      421 caaacttatg tgatggacaa gtcagaggca caaacgttgg tgatttcact taaggataga
      481 gaaacagcga tttatttcga tttgttctat accatattta ctgatagagc agtgatcaca
      541 cgatctgtaa agataagaaa tgaaaccgga gaaacaatca aactagaaaa agcggctagt
      601 ttccagctag actttgccca tactaggcga tttgatgaag tcattgcttt gccaggagca
      661 catgtcaatg aaagacagat ttcacggcaa tctgttttat ctggaacaaa agtatttgaa
      721 agccgtcgag gaacgagcag tcaccatatg aataacttta ttgctttagt tcatcatcat
      781 acgacagaaa atactggaga agcgatcgga cttcaatttg tttattccgg caaccattct
      841 ttcgaacttg aaaaagatca gatcaatcaa ttgagagtcg tcggtgggat caacagccat
      901 cgtttttcgt gggaattgaa tgcaggccag tcttttcaaa cacctgaaat gatcctttct
      961 tactcaagtc agggattgaa caaaatgagt cagatccacc atgagttgct acgtgaaaga
     1021 atcgcaagag gtcgtcatca atttgctgaa cggccgattc tggtcaataa ctgggaagcc
     1081 acttactttg acttcaatag tgaaaaaatc aaggcgatca ttgatgaagc aaaggagcta
     1141 ggcatcgaaa tgttcgtatt agatgatggc tggtttggaa aaagagatgc ggacaattct
     1201 tcattaggag actggttcga gtatgaaggc aagttgacaa atggattacg tgaaatagca
     1261 gattatgctc atagcaaagg attgaaattt ggtttatggt ttgaacctga aatgatctcg
     1321 gttgattctg aactgtatcg aacacatcct gattttctta tgcaggagcc aggtagaatg
     1381 ccttctgctt ccagatcaca acatgtactg gattttacaa ggttagatgt tcggcagacg
     1441 attgagaaac aaatgagaaa gattttagat acgattcctc ttgactacat caaatgggat
     1501 atgaaccgtt cgctttcaga tgtctattcg attacccttg acccacagag acaaggagaa
     1561 gtagcgcatc gttatatgct cgggctttat gaactacttg aacatttgac aactgattat
     1621 ccggaaatct tatgggaagg ctgttctggt ggaggtggac ggtttgatgc cgggtttatt
     1681 tattatatgc cgcagtcatg gacaagtgat aacacggatg cagtggagcg tatgaaaatc
     1741 caatatggaa caagccttgc ttatccgatt tcgtccatca cagctcatgt atcagctgta
     1801 ccaaatcatc agaccgacag atctacttcg ttgaaaacaa gaggagaaac agctatgagc
     1861 gctgtttttg gttatgaact ggatttgaca aagctgtctc ctgaagaaaa aaagcaagta
     1921 aaagaccaaa ttatatccta tcaaacgatt cgtccggtga tccagtatgg ccattattat
     1981 cgtttggcga gtccttttga agaaaatatt gccgcatgga tgtttgtgtc tcctaaacaa
     2041 gatgaagcta ttgtgttttt agggaggata ctagcttcag ctcaaccagc gttccatgaa
     2101 gtatatctga tggggttaga tgatgaggca ctttatcagg aacagacctc gaagcggata
     2161 ttttcggggg ccgaattgat gacagttgga ctttacttcc ccgattttca aggtgatttc
     2221 caaacagaac tgcttcattt caaaaagtta tgagagagaa ggaaaaaagt atgaaagcaa
     2281 atataaaatg gttagatgat ccacaagttt ttagagtgaa ccaatcaaat gcacatagcg
     2341 atcatgtttg ttatgcaaca agggaagaag tagaaaccac atcctccctt aaacagtctt
     2401 taaatggatc atggcgtttt tgttattcgc caaatccttc ggtacgacca aaaacatttt
     2461 ataaaaaaga ttttgatgcc tgttcttttg atgaaatcac tgttcctagt catattgaat
     2521 tagcaaatta tgacaagatc cattacatca atacgatgta tccgtgggag ggacatgtat
     2581 ttcgccgtcc ccctttcacg acaagccctg agaaagaggc ggaagggatc ttttcagctg
     2641 ctaaagacaa tccggttggt tcgtatttga cgacttttcg attagaagaa ggtctaaagg
     2701 gaaaacaaat ctcgattcat tttgctggtg tagaacaagc aatgtttatt tggttgaatg
     2761 gtcattttat cgggtatgcc gaagattcgt tcactccgtc tgaatttgat ttaacacctt
     2821 atttggaaga agaaaatatt ctagctgtgg aggtttacaa aagaagtaca gctgcattct
     2881 tggaagatca ggactttttc cgattttttg ggatcttcag agatgtcttc ttagttgctc
     2941 gaggacaaaa ccatttagaa gatctttgga tcaaacctcg cttagatgac aaatgtcaaa
     3001 atggcaaact tgaagtagat ttgtcttttg agcaagtagc caaaaacagt cagctgaatg
     3061 tagcgatact agatcctgat gggaaggaaa tctatcaaca gcaatatccg ttaaaagagc
     3121 aaatgacgat tcgcatcgat ggatttgaaa atgtggcact gtgggggcat tttcagccca
     3181 atttatataa gcttgaattg gagacgatag acgaagaaaa aagagtaacg gagtatattg
     3241 actattcctt cggttttcgt aaaatcgaaa tcaaagataa aatcatctac ttcaatggaa
     3301 aacggatgat tatcaacggg gtaaatcgac atgagtggca tccgaaaaaa ggacgcgcca
     3361 tcactataga agatatggaa aaagatttgg ctatcataaa aaatgcaaag atcaacggcg
     3421 tacggacatc tcattacccc aaccagatca cttggtatta tttatgcgac caagcaggaa
     3481 tccatgtgat ggcggaaacg aacctcgaat cccatggctc atggcaaaaa atgggcgcat
     3541 ttgagccttc ttacaacgta ccaggtgcag tacctcagtg gcaggcggct gttcttgatc
     3601 gagcaaaaac gaatttcgaa acattaaaaa accatccaag tattcttttt tggtctcttg
     3661 gaaatgaatc ttatgcggaa gaaaacattc gacaaatgaa cgattacttc aaaaaagtag
     3721 accccgatcg acttgtccat tatgaaggga attttccaaa tccagcattc gaagattcga
     3781 tttcagatgt aaaaagctgc atgtatgcgc caccagaaga gatcattgac taccttgaaa
     3841 ataaagcgga aaaaccgttc atcttatgtg aatatatgca tgacatgggg aattcgttag
     3901 gtggaatgga ttcttacatg acgttgcttg atcgttatga aatgtaccaa ggaggctata
     3961 tatgggattt taaagatcag gcgatccaag tgactgatcc aatcacaggc agagaagtga
     4021 tgcgctatgg cggtgacttt gacgataaac caagtgacta tgaattctca gggaatggga
     4081 tcgtttttgc agatggacaa gaaaaacccg ccatgcagga ggtaagatat tattatgaaa
     4141 aatacagtaa ataaaagtca tatggatacg gaaaaagttg caatcgtctt cggcgactgt
     4201 acattaggtg tcaaatcggg gaatacgcat tatatttttt cttatacaag aggcggactg
     4261 gaatcgctca ataaaaacgg aaaagaatgg ctataccggg aaacacttcc agttttttgg
     4321 cgagcaacga ccgataatga cagaggtagc ggatttggat ttaaatccgg tatgtggtta
     4381 ggagccggat tgtatccaaa agtcaaagaa atagaagtac tggtggacaa taaaaagatc
     4441 gatttgccga ttgctccagt aaataatcaa tattcaagta atgaatacgc tgaaacggtt
     4501 gaaatcattt ttacactgga aatcaacaca gtaccaaaaa cccaagtgac aatttcctac
     4561 caaatcgctg gagagggtaa aatgcttgtc aatatgcatt attatggaca aaaaggatta
     4621 ccggaattac ccgcattggg tttaaggttc attatgccaa cgaaagcaaa attttttgag
     4681 tatgaaggcc tatcagggga aacatatccg gatcggatgg ctggtggtgt cgaaggtatt
     4741 tataaagtgg caggactccc cgtttcccca taccttgttc cccaagaatg cggcatgcat
     4801 atggaaacga aatgggtaga actaacaaga aatcaaacgt tggataatac ggataaagat
     4861 caagaagatt tttcgttgcg atttgaacag caggatacgc catttgcttt ttccgcttta
     4921 ccattcaaag cagaagaact ggaacaggca acacatatag aagaactacc gccagaaaga
     4981 cgaacggttc ttacgatttt tgcaaaagta agaggagtcg ggggtatcaa tagctgggga
     5041 gcggatgttg agtcacctta ccgaatcagc agcgaagagg accatgaaat cggctttatt
     5101 attcagtagc aatctttaga ttattatttg aaaaacggag tttacaagac aaagctgctt
     5161 tattctatat gtcatgaaca agagagtaaa aaagttctca gatac
//
LOCUS       DAGCWU010000162         5199 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.164, whole genome shotgun sequence.
ACCESSION   DAGCWU010000162 DAGCWU010000000
VERSION     DAGCWU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.164"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            1..357
                     /locus_tag="MBZ71_002422"
     CDS             1..357
                     /locus_tag="MBZ71_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HBT4430325.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            373..723
                     /locus_tag="MBZ71_002423"
     CDS             373..723
                     /locus_tag="MBZ71_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HBT4430326.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            752..1531
                     /locus_tag="MBZ71_002424"
     CDS             752..1531
                     /locus_tag="MBZ71_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HBT4430327.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1542..2219
                     /locus_tag="MBZ71_002425"
     CDS             1542..2219
                     /locus_tag="MBZ71_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HBT4430328.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2238..3344
                     /locus_tag="MBZ71_002426"
     CDS             2238..3344
                     /locus_tag="MBZ71_002426"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBT4430329.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3334..4425
                     /locus_tag="MBZ71_002427"
     CDS             3334..4425
                     /locus_tag="MBZ71_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBT4430330.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4438..5178
                     /locus_tag="MBZ71_002428"
     CDS             4438..5178
                     /locus_tag="MBZ71_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBT4430331.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1659 a          858 c         1246 g         1436 t
ORIGIN      
        1 atgattaaag tagttattgc agaccgtatg ggtaaaggac aaaatgtggc caaaggggta
       61 gaagcagctg gtggaaaagc agttgtcgta ccaggaatgg gggcagatat gcgtttgggc
      121 gatgtgatgc aacaagagca tgcagatatg ggtatctctt tttgtggtag tggtggtgca
      181 ggtgcattaa cagcagcaac aaaatatggc tatccagaac gacatggtat gcgttcgatt
      241 gaagaaggga tcacagctat caaggatggc aaaactgttc taggtttcgg ctttatggat
      301 caagaggaat tagggaaacg tctgacagaa gaatttatca aaaagcatgg aaaatagaaa
      361 gagggacatg aaatgagcgt tcaaaagaca attcagcaaa aagttatcgt ctccggtaaa
      421 ggggaaacaa agcaacatgc ttttgcatca gcgttagcag aaattcaaaa caaaataatg
      481 gaacaatcaa acgtattatt gcgaatcgaa ccagtcaatg tggatatttt aaaggcggtc
      541 gaaaacaaat acacagaacg ctttctattt ttcttcttcc cacggcaacg tattagctat
      601 gaagtcgaac tctctgttac tgtggctgta acagaaataa aattggcaga catatcgttc
      661 agccagcaaa tgactgaaga tccaaatggg attgccattc catttgtaac taaaaaaatc
      721 taattttgaa tcataagggg gaaagaataa cgtggatttc gtaatcattc tagtcaaatc
      781 ggtcattatc gggggattac ttggttttgc agcatctgtc ggggcagctc gaatgtttca
      841 tgcacctaat acacaaggtt taggagcttt tagaacatta ggcgaaatga atgcctgcat
      901 gggtgatcca gcttctcatt tttcatttgg tttaggcttt ttctttaatg catgggcttc
      961 ttcagttggt gctggtgcat tcacacaaga tgtgacgcat cgaatcattc ccaattgggc
     1021 agcggcagca cttctagcaa aaaacaaaga tgtcacccag accatgcatg atccgaaaaa
     1081 aatggggatc attggagcgg ttatcggggc agtggtggta gccttcttaa acacaacagc
     1141 tgcagcaatt ccagaggcgt tacaagtgac ggcggtttcc gttttggtac cagcagcgac
     1201 gattctgatc aatacagtaa tgccagttat tttttggttg gcagctttag acgcagggaa
     1261 acgaactggt ttttggggca ctctttttgg tggtatcgcg caattgatta tgggaaatgc
     1321 ggtacctggt gttgttctag gaatcttgat tggtaaaggc gtcgatgaga gcggttggaa
     1381 tcgcgtcacg aagagcatga tgatcgctat tgttttatta tttattttca gtggattttt
     1441 ccgtggattt gatatggaaa tgatcaagag cttccagctt caagtgccta attggcttga
     1501 taacttgcac aacatcttta gtgtgaaata ggaggagaat catggagaca acagaagaac
     1561 aagcaaaagt attagaagca aagcggcaaa agagcttttg gtttgcagac tggtcttttc
     1621 cgctaatcgt aggaattatg tccgctgctg tatttgcagg aacacacatg tatgtgatgt
     1681 atggtgtggg tgcattcaat gaagtttcga tcgtggcgat gttaaaagcc gggctggatg
     1741 gcggatccta tggcgcagcg gcagcgtttg gtgctagttt cttatttgcc agaattttag
     1801 aaggctcttt agttgggatc ttggacttag gcggttcgat cctaactggg atcggaattg
     1861 gggtaccagc aattctctta agtatgaaaa tagcggcacc aatcgataat tttgcactct
     1921 ctttattgac tggtttggtt ctaggtttga ttgttggtgg catcattgtc ttgattcgta
     1981 aatttacgat caatcaatca aattccactt ttggtgctga cgtcatgatg ggggcaggaa
     2041 attcatctgg acgttttttg ggtccattaa ttatattaag cgcgtgtgca gcatctattc
     2101 caatcggcat tggcgctaca ttaggagcac taggattcta tgcttggaaa aaaccaattg
     2161 ctggaggtgc tattttagga gcaatgattt taggagcaat cttcccggca gtaaattaat
     2221 agaaagaggg tcattaaatg tttgatactt tgattaaaaa tggaaggtta gttgatggat
     2281 ctaaaataga ggtcgctata gaaaaaggca tgattaaagc agttgcagca gaaataaatc
     2341 agccggcgca agaagtagtt gacttggagg gaaagtatta tttatccgct gggtggatcg
     2401 atgatcatgt ccactgctat gaaaagatga atctttatta tgactatcct gatcagatag
     2461 gggtcgaaaa aggtgtgacg acggtcattg atgctggaac tacgggagca gaaaacatcc
     2521 gtgaatttta tcaattgacg aaatcggtca agacaaatgt gtatgcgcta atgaatatct
     2581 ctaaatgggg cattgtcaaa caagatgaac ttgctgatct aacaaagatt caagaagatc
     2641 tagtaagtac tgcgttagca gaactgccgg aatttattgt cgggattaaa gcgagaatga
     2701 gtaagacagt tgttggtgaa aatggcatta agccattaga attagctaaa aaaatccaga
     2761 cagaaaacaa tgatctgcca ttgatggttc atatcggttc tgcacctcct gagttagaag
     2821 aagtattgtc aagaatggaa agaggcgatg tattgaccca ttgttttaat ggaaaaccaa
     2881 acggtatttt agatccaaag acagatcaaa ttaaaaactt tgtctgggat gcttacaata
     2941 atggtgtcgt ctttgatatt ggacatggga cggatagctt taatttccat gtggcagaaa
     3001 ccgcactaaa agcaggaatg aaagctacct ctattagtac ggatatctac atccgcaatc
     3061 gtacaaatgg cccagttttt gatctagcaa ctactttaga aaaattgagg gtagtcggct
     3121 attcttggga agaaatcatt gaaaaagtta cagcagttcc tgcacggaat tttcatttga
     3181 agaataaggg ccagttagct gtcaattatg atgcagattt gacgatattt gagattgttg
     3241 aaaatgaaaa ggaattgaaa gattccaacg gatttacacg tatagctaaa gaacaaattt
     3301 taccgattaa aacaattatt ggaggcgtca tatatgacaa ttagctatgg aaagtttgat
     3361 ttaaaagaag tcattaatgc atcgggaaaa atgacgatcc taggggtttc taaggtatct
     3421 gaaagtgtcc tagcagcaca acgatttgga ggcgagcatt tttttgagat gagtgatctt
     3481 gccatcaaaa ccggcgctta tctttctcaa ttactgaaag tggaagatgc gcaaatcgtt
     3541 tcttctgctt cagcagggat tgctcaatct gttgctgctt taatcggaca gggcgacatg
     3601 taccatgtct atcatcccta tacagaaaaa atcgctagac gggaaatcat catacctaag
     3661 ggacataatg tagattatgg gacaccggtt gaagtaatgg ttgctcaagg tggaggaaaa
     3721 gtcatagaag ctggctatgc aaatatgtgt acaccggaac atgtttccat gatgattacg
     3781 gaaaaaactg ctgcgcttct atatattaaa agtcatcatg cagttcagaa aagtatgctg
     3841 acggtggcag aaatggtaga agttgccaaa gctcatcgtt tgccattgat cgttgatgca
     3901 gctgcggaag aagatttatt caaatatagc gaaatgggcg ttgacttagt gatttactct
     3961 ggggcaaaag caatcgaagg tccgagtgcc ggactggtca taggaaaaaa gaagtatatt
     4021 caatggattc ggctacagtc taaaggtatt ggccgagcaa tgaaaatcgg taaagataat
     4081 attttaggct ttacccaggc actagaagat tatttaaaaa atgggagcga atccggtgac
     4141 tccatgaagc aacgattagc gccttttgtt aacgcaatca atcaacttcc tgacctttca
     4201 gcgaaaatag ttcaagatgg tgccggcaga gatatttatc gagcaagtgt aacgattaat
     4261 ggcgataaaa gtgcgaaaga agtcattcag gaattaaaag cacgaaatcc agcagtctac
     4321 acccgagaat atcaagcaaa taacggaatc attgagttcg acattcgttc cgtcagccaa
     4381 aacgaaatgg aaaaaattat aaaaagattg agagaaatca tgtaggaggc ttttaaattg
     4441 tcagttatcc caaattatta taaaaatcgt gtatgtttaa atgtattagc tcattcagtt
     4501 gaaaatgcaa aagcatgcta tgaagcagcg gaagggcatg tcgtactagg tgtgctatcg
     4561 aaaaattatg ctagcgatga agcagcaatc gaagatatga aaaggtacca agacgcaacc
     4621 aataatgcgt tgtcagttgg attaggcgct ggagatccaa accaaagtca gatggtttcg
     4681 cgtttatctg gggttttaca accacaacat gtcaatcaag tatttactgg cgtggggact
     4741 tctcgtgctt tattgggcca aaatgataca gtcatcaatg gattggtttc accaacaggt
     4801 aagatcggct atgtcaatat tgctacaggt ccgttaagtt cacaagcgcc agcagctgaa
     4861 gtgcctattg aaactgccat tcaaatgctg caagacatgg gaggtacttc aatcaagtat
     4921 ttcccgatga aaggcttagc acatagggaa gaatttaaag cagttgcgga agcttgcgca
     4981 aaatatgaat tctatttaga gccaacagga ggaattgatc tttcaaactt tgaagaaatt
     5041 ctgaaaattg caatcgatgc aggtgtaaaa aaagtgattc ctcatgttta tagttcaatt
     5101 atagataaag aaacaggaga tacgcgtata ggagacgtaa agatactatt aagcatggta
     5161 aaaaaaatta ttcactaact cattagaaaa taagaagct
//
LOCUS       DAGCWU010000163         5152 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.165, whole genome shotgun sequence.
ACCESSION   DAGCWU010000163 DAGCWU010000000
VERSION     DAGCWU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.165"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(41..1189)
                     /gene="nagA"
                     /locus_tag="MBZ71_002429"
     CDS             complement(41..1189)
                     /gene="nagA"
                     /locus_tag="MBZ71_002429"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBT4430332.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1265..2101)
                     /locus_tag="MBZ71_002430"
     CDS             complement(1265..2101)
                     /locus_tag="MBZ71_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4430333.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2285..3502)
                     /locus_tag="MBZ71_002431"
     CDS             complement(2285..3502)
                     /locus_tag="MBZ71_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4430334.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3651..3830)
                     /locus_tag="MBZ71_002432"
     CDS             complement(3651..3830)
                     /locus_tag="MBZ71_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430335.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3885..3968
                     /locus_tag="MBZ71_002433"
     tRNA            3885..3968
                     /locus_tag="MBZ71_002433"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3919..3921,aa:Leu,seq:gag)
     gene            complement(4208..5065)
                     /locus_tag="MBZ71_002434"
     CDS             complement(4208..5065)
                     /locus_tag="MBZ71_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430336.1"
                     /translation="MIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLA
                     ELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLF
                     FLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLN
                     NLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCS
                     RLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGIC
                     TFIITVINR"
BASE COUNT         1596 a         1048 c          819 g         1689 t
ORIGIN      
        1 aaacttaaga agtttagttt taaggttaac ttaaattata ttatgcgtgg aaacgttcca
       61 cgccatctag ataagttgct tccaatgtca tatcagggtt caatacgata aagtctgcat
      121 cgcgtccttg agcgattttt ccacatgtat catcgatttt acagctgatt gctggaatca
      181 gactagccat catgactgct tgttctggtg ttgctaatcc ccagtctaca acatttttaa
      241 ttgcttcttt caatttcaat atacttccag ctaagttgcc agattctaga cgtgctgttc
      301 cttcttttac tactactgga aattcgccta aattgtaatt tccatcaggc atcccaccgg
      361 ccatcataca gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc attaggattc
      421 ccgcagcatt tggatgtaca tgatggccat cacaaatcaa ttcagaaaag acatgttcta
      481 aagataataa cgcacccacc atacctggtt cgcgatgatt caatccacgc ataccattga
      541 aagcatggac aaatacactt gcgcctgctt caacagcttt ttgcgcttct tctagtgtag
      601 cattactgtg tcctagagaa acaactactc cttcttcagt cacttgtttg acaaactctt
      661 ctacaccttg acgttccggt gctaaagcaa tttttttgat taaaccacct gaagcttctt
      721 gccattcatg gaaagtatcc aaatcaggat ccccgaaata acttgggttt tgtgcccctt
      781 tgtgttcttc tgtgaagaaa ggaccttcaa aatagattcc tttgatttta gctcctggtg
      841 cttcctgata cattttgccg attgtctcag ctacatcttt taaacgttct ttgcttgacg
      901 tcaatgttgt tggtaaaaat gaagtcactc cacaagataa cagaccttca gacatcactt
      961 tgatgccttc tgcatcatta tccattacat catgattcat atagccgtga atatgtgtat
     1021 ctaccaaacc aggagcaatc catttgccgc tttggtcaat gatgttgact tctccctcag
     1081 gtagttcttt ggtgtattca ccaaaaagtc catcggtaat ttcaagatag cctgcatgtt
     1141 tgacacctga cgctaaaaag aatttgtcag catagataaa agttctcatt ctattctctc
     1201 cctttgtttt tctacctcta aagtactctt atttagagaa gaagtcaaga atggtctaca
     1261 ccatttacaa attttttgaa atttgtttca cagcttgtgc taagccttca tagaatgctg
     1321 ctctttctgg ttcttcttta cgcttgtata ttaagctttt aagcacaaat aaatcattga
     1381 ggtaatagta gctattcttc tcttgagccc aatagattcc ttgattcacc tgttctaacg
     1441 cttcgtcgat ttcattttga tggaataaga aatttccata attataaaaa atttgcgaaa
     1501 attcactttt tgatcgttca tcagctgatt gatgcagcag ttggatgctt cgactcaaat
     1561 agtagcgtgc ttcagccgtt tttcctgcca caccatatat ttttccgata caactaatca
     1621 gttgtatttc tgtgtcagaa acatggattc tttctttttg gctagagtag cttaatgctt
     1681 ctttcaaata ataaagggac tgagagatat tttgacgcaa cgtgaattcg caaacaccga
     1741 gataataata atagcgttga aagtcagttg cttgatacag attttcagaa atatcagaag
     1801 attttagtac ttgttctaac ttccgataat cacgtttttg gaaacaacta tccaacagtt
     1861 caaataattt atcgtttgtc cgagtagttt ttcgagagag attcatcaca tgatcaatcg
     1921 ttacccctag ccgttgacaa atctgcatca tcaccaatac attcggcagt tcctcattat
     1981 tttcgatccg acttaataca ctttgtgaac aaatatcatg cgaaagcatt ttttgcgtca
     2041 ttttttgttc ttttcgaatc tctttaatga tcgttccaaa ggactcgatt aacgtttcca
     2101 taagtatcct ccccaaaata tgcgtattcg cattctattc gtattttact ttaggaagtc
     2161 gcaaacttca agaaaagact tgcaactaaa taatatactt tagttattta agaaaactcc
     2221 actagaaaga gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa tttggttttt
     2281 aaaatcattt ttggcgatag gttgctagga attctctcat tttcttagct gtttgtttca
     2341 aatcatccat tttaggaaga taaacgattc gaaaatgatc tggctggttc caattaaatc
     2401 caccaccatg tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg aatttttcat
     2461 catccaaaat attgaatcgc tttgtatcaa tttttgggaa tatataaaaa gcagcttttg
     2521 gttttacggc tgaaagacca ggaatatcat tgattgcatt atagatatat tctctttgtt
     2581 catagatcct tccgcctggc aggagcagct catcgacact ttgataaccg cctagcgcgg
     2641 tttggataat ttgctgagac aatacattgg aacacaaacg catggaggac aacatattca
     2701 gcccttctat atagcctttg acgtgtgact tatctccact taggaccatc cagcctactc
     2761 gaaatcctgc tacacgatga gatttggata gaccgttcag cgtaacaacg aacaaatcag
     2821 gcgctaatgt agcaatcgga atatgttcta gaccatccat cactaagcga tcatagattt
     2881 cgtcggaata aataatcagg ttattttgtc gagctacttc aacgatttct tctaataatt
     2941 cttttggata aagtgcccca gtaggattat taggattaat gataacaata gcttttgtcc
     3001 gactcgttat ttttgatttg atatcatcaa tatcaggata ccattctgcc tcttcatcgc
     3061 aaatataatg aacaggtgtg cctccagcta atgcaataga agccgtccac aacggatagt
     3121 ccggcattgg aaccagtact tcatctccat tgtccagcaa cccctgcata cacatcgtga
     3181 tcaattcgct cactccgttg ccggtgtaga tatcattgat cgtcacattc ggaaaatgtt
     3241 tcagttggca atattgctca attgcttttc tagcagaaaa gatccccttg gaatctgagt
     3301 agccttctga gtcacgggca ttcatgatca aatcacgaat gacttcgttt ggtgcttcaa
     3361 atccaaaagg tgcgggattt cccgtgttca attttaaaat actaatgccc tcttcttgca
     3421 tccgctccgc ttcttctaga acgggaccgc gaacatcgta actgacacct tctaatttgt
     3481 tggatttttc aaattctctc atcacttagc caatccctca tttcaaaaag ttagaacttt
     3541 ccaaattttt tgttatttac tatttcaata tcatactcca tcctagaaaa ttttgccact
     3601 tatttaggaa aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac ttaattgtca
     3661 tgatagtcat tccatagatt cgctgcccaa tccattgcaa ttccacaaat gattcctagc
     3721 ataaaaccaa gcaaaaaacg tccgaatgca agtccaatca atacacctaa gatcattccc
     3781 agtcctgaaa attctccgtc atatttctcc atcttaataa gtcctcccat ttttgagctt
     3841 gacaactatg taaaacctcg ctatcattag tgtgttatta ttacgcagat gtgttggaat
     3901 tggcagacaa gcatgattga gggtcatgtg agcgttagct cgtgtgggtt cgagtcccat
     3961 catctgcact acaactcgtg aagttaattt tgcttcacga gtttttttgt ttgaacgttt
     4021 ctttatgcca ttatatacat atcactgtat cctttacaaa aagaacgtac aatcatgtaa
     4081 acagcctgag gtgagtatat gaaggtcgga aacaagctac tcgtagaaac ttgcaagtgt
     4141 taaaacaatt aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc gagggatttt
     4201 ttgtatatta tctgtttatc accgttatta taaaggtaca aataccaagc acggttaatc
     4261 caaattgcat aaattcattt ttttgtacac tcatgctttt cattagctct ttaactgcca
     4321 ttagtactaa gatggcgata acaacataaa gtatatagta cgtccgcatt ccggaagccc
     4381 atactgtggg agaaaatccc ataattaacc ttgaacaaaa tcccattatc atcaaaataa
     4441 ctaaaaaaat agaagttttc ttattgtcaa aacttaaata aattccaaca attaaacata
     4501 gtaataatgc caagactact aaagtagcta tccaagtacc tggataaaaa agtgacagtt
     4561 tcgttcctaa ctctgtaaag agattattca ggttactact actccaaatc attgcccttt
     4621 tatttccatt gacgtaagta aagctttgac ccattgtatt accaaaataa ataatcaggt
     4681 ttaagaaaaa cggtaatgca gtcaatatac gcacataata gttttgacat ttcctatatg
     4741 ttagaaggaa aattaagaaa aatagcaata aaaataagat attcatgtct aaaaaaaacg
     4801 gctttccaaa tgatgaaata cctaaatcta acttattaag aaagttgaaa tttttatatt
     4861 caggaaacca tttattaatt tcatgtgcag cacgtaatgc attccctgga gttgtcaaag
     4921 agaaaattaa ttctgctaag cttattattg aaaatactga taacttataa ttatatcgtc
     4981 cattgacaat caaatataaa ctaactatac ttgttagaac aaaaaaacag gcgttgactt
     5041 gttcttgatt agttaaaaat atcaataaag gaatacttac ccaatatatt tttctattta
     5101 tttcttctcc tcttaagagt tgaaaaaacg gataaaaagt tagtaaacta aa
//
LOCUS       DAGCWU010000164         5028 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.166, whole genome shotgun sequence.
ACCESSION   DAGCWU010000164 DAGCWU010000000
VERSION     DAGCWU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.166"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            28..987
                     /locus_tag="MBZ71_002435"
     CDS             28..987
                     /locus_tag="MBZ71_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4430337.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSAASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(1240..1389)
                     /locus_tag="MBZ71_002436"
     CDS             complement(1240..1389)
                     /locus_tag="MBZ71_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430338.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            1851..2063
                     /locus_tag="MBZ71_002437"
     CDS             1851..2063
                     /locus_tag="MBZ71_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430339.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(2341..4140)
                     /locus_tag="MBZ71_002438"
     CDS             complement(2341..4140)
                     /locus_tag="MBZ71_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBT4430340.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(4264..4860)
                     /locus_tag="MBZ71_002439"
     CDS             complement(4264..4860)
                     /locus_tag="MBZ71_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBT4430341.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1794 a          881 c          873 g         1480 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtaga tgaatttttt attcaatctg ccgcatctaa acgaaataat
      901 attcctagaa aatcactgga ttatcgaaca cctttggaag tatttttgag ttacgtaagt
      961 attgatgatc tgtctaactt aatttgacaa ttaagattat attaaatatt atacagataa
     1021 taacggaata ttatcatcga aacagctaac aaaattgtca taaaaaacga gatggttgat
     1081 attagaactt gtgtttttgt ttaggtctag agataaagag agacaatttc caaatatatg
     1141 taatgaatac aaaaagacgt ttgtcgcgga ttaccgttac aaacgtcttt tattgatttt
     1201 ccgagatctc atcagtcgcc atttggaatg gttcactcct tatctagatt cgtttaatgc
     1261 tcggactgct tgttcgattg taataccaaa cgcaacgtta ttttcgtttt gtgcatcaat
     1321 cgccataaaa cggttagaag ttgtatcaaa agctacgcgg tattgcatat ttgtttttgt
     1381 aaacgtcata aaattcatct cctttgtaat gtaatttagt tcttgaaagt ttcctttaaa
     1441 ctttcgaact tataccaagt ttagcatcgt acgttttttg atgcaattaa aaagcattta
     1501 tcattttcct tatttatttt ttcttaaatg gtttagctat tttcttttaa tccgataagt
     1561 agcccaatca tcaaggttac aaatactatt gcttgaatat aaatcatatg ctcacctctt
     1621 tataaataac ttagcaatat aaagaaaaaa acgcacgaac aacataggaa tataaacaaa
     1681 cgtttttgtc acaacgtcta agatcgtgtt aaaaaatagt tatctaaaaa gcagaaagat
     1741 actcaaaata aaaaataaaa aaatggataa aaacgttttc aaatacaagg taatttgtta
     1801 ttataaaata aagaaaaaac aaaataaaaa taaagaaaag agggaatgaa atggtaaaag
     1861 cagaagtagg aagatcttat atatgcaaac cgatcggagt atctcatgaa ctaatcggct
     1921 gtattgaacg tacatatatc aacacggctg tcgtgtatgt tgaaagttat gaccaacgag
     1981 actatcaact tatcaatgaa agcaagtatc gcatgctcgt aaaattctgt gatattagcg
     2041 cacctgctga attggtttct taaacagtat ttttgagaat tcgcaagaac tcccttataa
     2101 tataaataaa gacagaaggg agcgagaaga tatgccaaaa agaaaaagtt cagggaaaat
     2161 gggaattatt cttgcagtga tctctgcaat tcctgctatt ttagctattg ttcgtgcgat
     2221 gaaggaatca agagaaagtt aagcacacat tatgagcgtc aaacaaaaaa cgcatatacc
     2281 tgcggtggca ggttatgcgt tttttgttgt ataaagactt ttttcattaa cgatttttga
     2341 ttagccgact cccaaaacaa aatttagcag tttatctgaa taagtgattt cttcatctgt
     2401 ttgtttcaca ctttcattta gtagtcccat ctcatcggtt atgataccat ccacaccata
     2461 aaacatcatg cgactcatag catctgaatc gttgattgtc catacatata cttcttttcc
     2521 gtcttggtgg gcagaattaa tgaagttacg gttgatcgta ctatattcca ttgtcagaaa
     2581 atcagcaggc gtcacagggg gcccaacaat attgaaaggt aagatgtagc ccacataaaa
     2641 gtttggttct gcttctttta aatctgaaac ggtttggtac gtcaaacttt gcaaaatatg
     2701 atggttctct aagataactt tgcggtattt atggacaaat cgttcgacaa ggtcatccga
     2761 atctttttta gaggtcttga tctcaataag taatttttgg tttagcttat gtgctttttc
     2821 caaataagaa tcaaaagaac aaacaggggc ttgcactccg ttttcagtaa ctgtaagatt
     2881 tgtcaattct ttcaatgtaa gatcctgtgg tgttccattt acccctgtta agtgacgaag
     2941 atttgcatca tgcatcacta cgaaatgacc atcttttgtt tcctgaacat ccatctctac
     3001 ataatcagga tgatagtctc tgcttgtttt ttcaagtgct tctaaagtat tttgcgcccc
     3061 attttgggta ctcacacctc tgtgagagac agtcactggt tttgtctgaa cagctgaagt
     3121 cagataatcc atattgtaaa gacagacgcc aataccgaaa aaaactgcaa aaagagtcaa
     3181 accagtgttt ttcagcgcag agaaacgcaa gttgggggct tgtgggataa accacttagg
     3241 gatctcaggc aagaagcctt cgtcatccat gaaatcaaca atgatataaa aaatccctac
     3301 ggtagacatc acgatattga ataataaaat cccttgtaaa agcgtcatag caaacacagc
     3361 gctgatcaat gcatagtctg ggaaaaattg ttcaaccata ctttgtgcca aaataacaac
     3421 gatatatcct aaagaagaaa ggagtaaaat cgtaccgcca atcacaataa attggcccgt
     3481 gatggccaaa agacgcgatt tggtcaatga ccagctttcc cgaatggctg ctcgaaaagg
     3541 acgatcccgt agaatcattt ccggtaaagc aaaaatcagt cgaatcccaa tatagccaag
     3601 gaaaatataa acgagtaaaa aggaactgac gatgatccaa cgattagcaa aaataaaatc
     3661 cataatgaaa gcggggattt ttatttttga cagtagatca gaattaaaac ttaatccgct
     3721 gatcggtaga atcaataaaa aataagcaag gaaaaacaaa aaagtaatgg gtctcacttt
     3781 cttcagctga agtatcgtca agtgtaagag ttgcttcaaa gagattggtt ctttcttttt
     3841 aataaaaaaa acactcatca aaagaaaagt aaattcgaaa taaactaaca gtaaaatcgt
     3901 catcaaaact acgattaacg agagtagaac cccaggatgt tgagaaaaaa ttgaaggaat
     3961 attgtcataa gacaaatagt caattgcccc tcggtgtaaa ataaaacggg tcgtactgct
     4021 caataagggt aaacaaataa ataaaataaa gccatgcatc agcaagacat cacggaaata
     4081 agcttgtgtt ccttttaaaa agtcccaagc attacctaag ctacgtttca aataattcat
     4141 cctatcacct gctcttcaca ttatagcgca aatctaatga aaaaggaaag ggtgcagtgt
     4201 gttttgcaga ccctttccca gatttaacat tgtcaatcaa tgactaattt tacaaaataa
     4261 atattatcca agaagaccac ttagatattg acctccataa atcaaggcca tactatataa
     4321 gaaaatcgac agtggtttag caatcaaaat gattagtgta tatcgtttca ctgacatgtt
     4381 tgtcaatcca gccatcaaac ataacgcatc gtcaggagca actggaagaa aaatagctag
     4441 agcaaacagc ttctcaaaac ggttttgatt atccagccag ccaatatatt tgttatatgt
     4501 cttatcgcta atcatggaaa gtatgaatgg ctttccataa cgacgcccta gcagaaaaat
     4561 gataatcgaa ccaatgcaga ttccaatata gttatagata aatccagcat aagggccaaa
     4621 tatcaggaca ccagctgcag tactgatccc tccaggaatg attggaataa ctacttgcag
     4681 gatttggatg aagacaaaaa taatcggtcc taaaatgatg gaatcaccta ctaatcctct
     4741 taacgcattc aaatctttga aaacacctaa attaataaaa taaatcgtca gcgcgattga
     4801 taggcctaat ccgataatgg atatgaaatt tatcagtttt cgagaggcag caatattcat
     4861 tattcatcaa ctccttagag gttctttgtt aactatactt tactagaaaa gcaactaact
     4921 gcgccatttt ttgatatgac agaaaacaaa caattttgta agttcatcat aagtataaaa
     4981 gattgatctc aaaaaaacat ccaactaaag acttatttaa taacttgt
//
LOCUS       DAGCWU010000165         4995 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.167, whole genome shotgun sequence.
ACCESSION   DAGCWU010000165 DAGCWU010000000
VERSION     DAGCWU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.167"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(145..756)
                     /locus_tag="MBZ71_002440"
     CDS             complement(145..756)
                     /locus_tag="MBZ71_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4430342.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(879..1004)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1086..1802)
                     /locus_tag="MBZ71_002441"
     CDS             complement(1086..1802)
                     /locus_tag="MBZ71_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBT4430343.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1808..2377)
                     /gene="scpB"
                     /locus_tag="MBZ71_002442"
     CDS             complement(1808..2377)
                     /gene="scpB"
                     /locus_tag="MBZ71_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBT4430344.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2361..3158)
                     /locus_tag="MBZ71_002443"
     CDS             complement(2361..3158)
                     /locus_tag="MBZ71_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBT4430345.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3128..3499)
                     /locus_tag="MBZ71_002444"
     CDS             complement(3128..3499)
                     /locus_tag="MBZ71_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBT4430346.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3518..4405)
                     /gene="xerD"
                     /locus_tag="MBZ71_002445"
     CDS             complement(3518..4405)
                     /gene="xerD"
                     /locus_tag="MBZ71_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBT4430347.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4501..4974)
                     /locus_tag="MBZ71_002446"
     CDS             complement(4501..4974)
                     /locus_tag="MBZ71_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBT4430348.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
BASE COUNT         1373 a         1091 c          852 g         1679 t
ORIGIN      
        1 tttcacattt gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca
       61 aacaaataaa acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa
      121 gaaatttctt gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg
      181 ttgttttcga cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac
      241 gatgaatcct ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga
      301 catgcccagc atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac
      361 gaaataattt gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa
      421 aaatttgttt cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt
      481 aaagatcgtt gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc
      541 tgtagtcagc agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc
      601 gaataagaac atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa
      661 agccggcatg atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc
      721 tactgcaacc attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc
      781 tcttcagcga actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa
      841 tagagttaga gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact
      901 atactgtcgg tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac
      961 tataaccacc ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt
     1021 ttacatattc attatacata tctattcaaa aaacgcaaca tctaacactt ataaaaagta
     1081 agtgttcatt ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa
     1141 tccccaggac gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc
     1201 acaggaagac caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc
     1261 gtcaattcca cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct
     1321 ggagatgttt ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt
     1381 gtagcgatcc cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc
     1441 aggcgctgag caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc
     1501 agtcgaccta caggatagat acgttctttt accatcggca aaagatccgt aacaactttt
     1561 cttcctttgt catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa
     1621 ccgtattctt cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact
     1681 tttatcccta attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat
     1741 tcttctgctt ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc
     1801 attacaatta ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt
     1861 caagtgaaat ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat
     1921 aatctaagaa gtagccagtt gttccatata atatcggccg acctggtcct tctactctac
     1981 ctttttcttc aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc
     2041 ctcgaatctg ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg
     2101 tttcgacggc ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg
     2161 gagccagctc ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag
     2221 ctgagtgcga gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg
     2281 cttcgctgag atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa
     2341 taaaaagaag ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga
     2401 taaagcataa ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc
     2461 aattctagca aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa
     2521 gaatcgaaag tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt
     2581 tcttcgatcg ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta
     2641 cgttctaaga ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc
     2701 aacggctttt cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga
     2761 cttcgttcct ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct
     2821 aaaagctgag tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg
     2881 acgaattctt ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc
     2941 actaagtatt ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata
     3001 tattgctccg tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc
     3061 agatgaagca gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc
     3121 aactcattca ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga
     3181 tttcagttgc acggatggag acatccgggt acttttcctg taattcatct aacatttgaa
     3241 agcccatatt ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat
     3301 cgttcgttgc ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa
     3361 agaaaaaaag ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag
     3421 ttttacctgt cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt
     3481 tccggtaatg tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct
     3541 tataaacatc cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat
     3601 ccgcgtgacc taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg
     3661 tggcaaagct atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta
     3721 ctaactgctt cagatttttc cagattcctt gccgagaaag acctttccca tgattattga
     3781 caaatacatg catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc
     3841 gttccagcca ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc
     3901 caactgtctg taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt
     3961 cactgacgcg catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta
     4021 acgtttttgt cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac
     4081 ttggtaattt ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga
     4141 tacgctcttg ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag
     4201 ttgctgtcgc ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat
     4261 cgacatcctg ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat
     4321 cacgttcata gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga
     4381 gataatcgtt gatttgctct ttcataataa tccccctcat gcctctctat catatcagat
     4441 accgcttcat tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac
     4501 tcagacggac tggtttcttt tttctttttc atagcattcc tgacagattc catggaacgt
     4561 tagccgatga tcttttacta aaaaatgata acgttcttct actacttgct ccacttcaag
     4621 taaaagatcc tcttctactt cctcgatcgt gccgcagttc aagcatagta aatgatgatg
     4681 gaaatgtttg gcgccttctt ttctcaaatc ataacgggct aatccatcat taaaacttac
     4741 tttatcgaca attttcaatt ctgtcaagat ttcaagggtt cgataaacag tagcaagtcc
     4801 aatttccgga ctcttttttt tcactaaaaa gaagatttct tctgcagata aatgctcttt
     4861 ctcattttcc aaaagcacaa gcagagtagc ttctctttgc ggtgtcagct taaaccccgc
     4921 ttcatgtagc tgctgttttg tttttttcat cgccgaaata gcattcatga ccatttactc
     4981 aaccccaaca attta
//
LOCUS       DAGCWU010000166         4861 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.170, whole genome shotgun sequence.
ACCESSION   DAGCWU010000166 DAGCWU010000000
VERSION     DAGCWU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.170"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1219)
                     /locus_tag="MBZ71_002447"
     CDS             complement(<1..1219)
                     /locus_tag="MBZ71_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBT4430349.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSFFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEM"
     gene            1280..1855
                     /locus_tag="MBZ71_002448"
                     /pseudo
     CDS             1280..1855
                     /locus_tag="MBZ71_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2036..3229)
                     /locus_tag="MBZ71_002449"
     CDS             complement(2036..3229)
                     /locus_tag="MBZ71_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4430350.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3313..3513)
                     /locus_tag="MBZ71_002450"
     CDS             complement(3313..3513)
                     /locus_tag="MBZ71_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBT4430351.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3937..4170)
                     /locus_tag="MBZ71_002451"
     CDS             complement(3937..4170)
                     /locus_tag="MBZ71_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4430352.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4177..4608)
                     /locus_tag="MBZ71_002452"
     CDS             complement(4177..4608)
                     /locus_tag="MBZ71_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4430353.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1325 a         1110 c         1003 g         1423 t
ORIGIN      
        1 acatttctac aaaaaaatag tgattctgtt ttgaaaattc gatttttagt tctgcatttt
       61 cgtaaacatc caacattttt ttcgcactgt atagacctaa cccgccattt tttttagagg
      121 tttttcctct tttgaccatc tccgatagcg tcaaattcgt tggatttgcc ttgttcgcta
      181 tactaaaacg tgatccctct ttttcttcca gcagattgat cgtaatcgga ctctgatcgg
      241 ctacacgatg atcgaatgcg ttagaaataa aaatagataa acagcgaata aaaacaacca
      301 gatcaatcgg gattgttttc acttcgacag gaataactac ttggatcggg attttctccg
      361 attctgcttt ttctagaaat tttaaaaaga cacccctgac agctggggat acaatatttg
      421 ttagctgttg tatttgcggt ttttcaaaaa tagctgcaga atgatcttct aatatttcta
      481 aaaattccaa cgtttctttt ttattttcct gaagagtaat ttttaatgaa aacagtagac
      541 ttttataatc atgtttgaat tcctctagat gatggtaata ttcttcattt atttgataca
      601 gttgaagcga ttcctgatgt tgtctttctt ttacttggaa actgatcaac ataatggaaa
      661 aaaaaaaaga aaaaaaactg atgactatcg acaaaagaga tagaagcaga taattagatg
      721 agcggaagtg aacacttccc aataccaagt aaatcacgcc ccagcttaat aaaatacaaa
      781 tacctccaaa atagtgttca gttggataag taaaaagcaa gttgagaaat ctcgtctgtt
      841 tgtctatact ttttactaat agatagaaaa taaatgcgat aagtagtgca aaaaaacttg
      901 ctgttccaag atgtctcttt cctaaataat ccatcagatc aaaaaccaga aaaaagctga
      961 gtccagtgat agcaaaaaaa agattactga tcaaacagct gataaacagg tttttcgtag
     1021 taaaataaaa aagaaacatc aataataagc caactaggta aggaaagctc actttaggga
     1081 taggaagtaa aagtgtcacc accatcaaag ctatacaacc aaagctgttt tttactagca
     1141 gttgtttcga aaagaataaa tagataccta aatctaaaat caaagtaaag gtcaaataaa
     1201 gtgtcactgt tgcgaccatc ttattcctcc attgttttgt ttcaaaatgt acacatattt
     1261 ttttaatagg agcctgcata tgaaaaagta ttttatcata gaagataacc agtttcacag
     1321 cgaatggatc atcagagaat tggtcaatca tcatatcgag aatatcgtgc agctcacttc
     1381 tatctctcaa atcaatcagt tgatccagtt gggcactatt gataatcacg atgttttctt
     1441 tatcgatatc gacttgaagc aatcactcac aggtattgat gtcgccgaac aaatacgtaa
     1501 aaaaaatgca catagtaata ttgttttttt caccgcttat acgaattttg cagtagagat
     1561 catcaatcgg aaaatccttc cgattggata tatcagaaaa gacaggaatt tatctgaaca
     1621 aattcgtcaa gtgctagaac agatcaattg gaaagaacag cagatcgtaa atgtcgataa
     1681 aatctttatt gatcaaaaag gtggaagtct cgtcctgtat cctcgggaaa ttttatatat
     1741 tgaaagtatc aaaggtttga aaaaccaagt tatggtcaaa acaactacag aagataaatt
     1801 agtgaatact tctttaaaac aattgaagac agagttagaa aagtatgctt actttttctt
     1861 aaaaattata tgggatttta gattatcttt ttagaagttc ttgaatgtcg gtatagcccg
     1921 tgtttccggg cttttccggc atttttgata ttgttagata cttacaaatg ttcttattac
     1981 ctctcaccat ttccccctca aaagtagtaa atggagtagt aaaaccggcc ttgtgtcagg
     2041 ctgccagccg ttccatttct gcccttgcgg aatcgaatgt ggcgtgtgca taatagttca
     2101 gcgtcatggt gatattggaa tgtcccataa tgtactgcaa ggctttgggg ttcattcccg
     2161 cattggctaa ccgggtgcag aatgtatgac gcatagcatg gggcgtcgtc gttttcggca
     2221 acgcttcttc atggctcttg ttgtacttct tcacaagcct gccaaacatc ccgccataat
     2281 ctaccgccgt catgggatag ccgttctgtt tgagaaacag gaaattggca tacccgtcaa
     2341 taatgaacgg ctttgcacct ttgcggtttt ttatcactcg ctggaacgcc tgatacactt
     2401 cctcattcat ggggattttt ctgacgccgc tttgggtctt gggttcgtca atatagtaac
     2461 cgacgccggt atttctcaaa agctggtggc tcacgatgat tacccggttt tcaaaatcca
     2521 aatctttgtc cgtcagtccg cacagttccg aaatgcgaag ccctgtcccc agcagaataa
     2581 cgattgcgtc ataatgcttt tggtaaacct tatcaccttg aataaaggac agaaaacttt
     2641 cttcctgtgc tggtgtaaga ggcacctttg gttccgtatc gtcgtccaga acgtcactaa
     2701 gctggaagtc aaacgggttt tttcggatgc aatcgtcctg tatggcggtg taaaaagccg
     2761 ctttcaggga acgcttgtcg ttattgatgg tcttataaga aaggcccttt tctttcatcc
     2821 gaagcgccca ttcctttgcg tcggacagct tcacactgtc aatcgggcag ctccccagcc
     2881 gatcttgttc cagaatacgc atcagccgtt cccggcccag tttggtgttg tgcttcacat
     2941 tgccccggtg cctgatctgc ttggcgtaga gctggcagac ggtcattttc ttgccgatgg
     3001 tgtctatccc gtcgtcaagg tctttctgga tttctttctc cttttcccgg agtgaaatat
     3061 cctcccgttt acctgccggg gtcttatccg taggcactaa cttccatgag taaacaaact
     3121 gcggttttcc aaaggtatca acatatttgt aagcatatcg tccgtctttt ctctggctct
     3181 ctccagaccg caaaacgcga tttttgctgt ctcgtctttt ttctgacatc tcgtcatgct
     3241 cctttccatg acggaaagag ccgcatatat gcttataaat tattataaca tatctgcggc
     3301 cctttttcac tattagattg tgtccaaagt gtcaattatt ttctcaaact gcttgcgttt
     3361 gatctggatg cggttgccat tcataatgac ccagccagag gccggatttt cctcggccag
     3421 cttacgcaat ttgttttctc cgatacggaa atatttagac gcttcctcaa tcgtcagcgt
     3481 gtatttttcc caaataggca catcatggtt cataatgcag ccccctttca caaattcaaa
     3541 ggtttgtgtg atttaggggc atagacggac ggctgctggc acagctccac gggactttca
     3601 cccccgcgtg gttctcacgc ggccctaccc attgcctgcg acgcttttaa cgctcggact
     3661 gtggctataa gggagtatca tagtcctgcc tgtacgtcgt cgcccgcaag ccgccacgct
     3721 cacattgcgg cgtggtgttc ctcgctccgc ttttcccgta gggaaaagta tcatggcgca
     3781 ccagccgtcc ggctcggcac agacagaatg tgtccgtatg ccctatgatg cgccgccgtt
     3841 atcttgcggg catatttatc aaggtgcgat caggccaccg ggaaaacacc gaaacgggga
     3901 aagggtaagc cgtttcatgc tgccggggcc ggttcgtcag ttcgtgacga tagcgtggat
     3961 gagcttgatt tccagacggc gcttcatggc ctcgtccaga cacacatgcg gatagccgtt
     4021 ttcgtcatag agcgtccggg tacacagctt gttcatgtaa ccgctgtaat actgcaaaac
     4081 gcgctctacg gctccggcat ccccagcgcg ggcggcctcg atcaccgaaa ggggcaaaag
     4141 ctcaatgccc ttgtagttcg ggttcttcat ccgcgtctaa cctcccttcg gcatcaacgc
     4201 ctttagctgt ttccgcagct ccgataaggt atttgtcctg tgccgctgaa cggcactccg
     4261 ggacattccc acaaggccgc cgatctcacc atctgccata tccagaacac agtgcaggat
     4321 taaaatactc tgctccatgc tgggcagcgc ggcaaaggct tcggcaacaa gctcattgtt
     4381 gatatgtagg tcatagccat gcgacgaaaa cgtaaagctg tcgctggggt agtgatctac
     4441 tgtacagagc ttgtccaaat ctgcctgtga caaagagctt agtgacactt cacggtcacg
     4501 gcggcgcttc atttcagcca agtagctttt ggcctcgtta cgcagtaccg tcttgcaaaa
     4561 agcatcgaac atgcaccgcg cttcatagtc gcgggggata gcatccatct tctcaccccc
     4621 tttctgtggg ggacttaggt ggttctctcc ctttccgcta acaagacttt gacaaggccg
     4681 ccagctaccc gctaaatacc tcctttttga aaatttctca aaaaattttt ttggggacag
     4741 ccgggccgca aaagacgaca aaaatcgccc ggcaggtcgc aaacctaccg ggcgagaaaa
     4801 tgctttgcta tgctgctgat ttacatggta tagtacatac tcataaccgg aatagccctg
     4861 t
//
LOCUS       DAGCWU010000167         4732 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.171, whole genome shotgun sequence.
ACCESSION   DAGCWU010000167 DAGCWU010000000
VERSION     DAGCWU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.171"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="MBZ71_002453"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="MBZ71_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBT4430354.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="MBZ71_002454"
     CDS             complement(2066..3064)
                     /locus_tag="MBZ71_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBT4430355.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="MBZ71_002455"
     CDS             complement(3290..3943)
                     /locus_tag="MBZ71_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBT4430356.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="MBZ71_002456"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="MBZ71_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBT4430357.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1389 a          994 c          742 g         1607 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata at
//
LOCUS       DAGCWU010000168         4675 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.172, whole genome shotgun sequence.
ACCESSION   DAGCWU010000168 DAGCWU010000000
VERSION     DAGCWU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.172"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(95..1030)
                     /gene="murB"
                     /locus_tag="MBZ71_002457"
     CDS             complement(95..1030)
                     /gene="murB"
                     /locus_tag="MBZ71_002457"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBT4430358.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1103..2023)
                     /locus_tag="MBZ71_002458"
     CDS             complement(1103..2023)
                     /locus_tag="MBZ71_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4430359.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2136..2888
                     /gene="xth"
                     /locus_tag="MBZ71_002459"
     CDS             2136..2888
                     /gene="xth"
                     /locus_tag="MBZ71_002459"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBT4430360.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3874..4407
                     /locus_tag="MBZ71_002460"
     CDS             3874..4407
                     /locus_tag="MBZ71_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBT4430361.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1380 a          915 c          839 g         1541 t
ORIGIN      
        1 tgtacaagat ttgcgccatt tgcgtaaaca accacaaaaa agagcaggac ggccaccgtc
       61 ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt ctgcttgttt
      121 ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac acatcaaatt
      181 tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc gctgtcgcat
      241 gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg ccccatttca
      301 ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact gggcgcttaa
      361 agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat tcggtcaact
      421 catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat cgagcttcca
      481 atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc atttcgtctt
      541 ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca aaaacatctt
      601 tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca cttcccggga
      661 tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa gtcgtatcaa
      721 tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc tgtttcattt
      781 cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga ttgcttgcat
      841 ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt tgctttactt
      901 cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc gtaaacgtat
      961 agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca actatttctt
     1021 ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc tatcacgctt
     1081 aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta agcacttcct
     1141 cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt tccgatttac
     1201 ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt ttgactagcg
     1261 tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcatcttga atgacaagct
     1321 gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat ttttctatat
     1381 tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt tctaattgta
     1441 aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt ctcaaaatgg
     1501 gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca taaaggacga
     1561 actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac cgcttatctg
     1621 ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg atcatcaaaa
     1681 ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga tggacactca
     1741 gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct aattcataat
     1801 gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa agctcatcga
     1861 tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt gtttctttat
     1921 ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt ggtaaatatg
     1981 ctttttctgc gatattccct acaccgatta caccaatctt catctacttt cctcctggat
     2041 catttagtta ctttcatctt acaccatttt ttactaaaat gaggtacaat agtatcgcaa
     2101 aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct tggaacgtaa
     2161 acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag ttagacgccg
     2221 attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta gatcttccag
     2281 gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca gcgatttttg
     2341 ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat gatacagaag
     2401 gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac acgccaaatt
     2461 ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc ttttataatt
     2521 atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta aatgtcgctc
     2581 atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga ttcactccag
     2641 aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca ttccgttatt
     2701 tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat tcaaggaaaa
     2761 acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag ccaggactag
     2821 ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta gaattagatt
     2881 tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa attttgtgaa
     2941 aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc ttttgaacac
     3001 tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     3061 aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagataca agcttttgaa
     3121 caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa
     3181 caaaaatagt tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
     3241 gaacaccgtt tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc
     3301 aaacaaaaat agttcctcat atttttcgtt tggttggact tattaccgca gatgcaagct
     3361 tttgaacacc gtttattgag aagttgtgaa atcagcgtcc tgacctgagt attagagaaa
     3421 caaatggcaa aatagttcct catattttgc tattttttga gctaatacgg agggtcagct
     3481 gattgaacac tgtttagtgg agaggttgtg aaatcagcgt cctgacctga gtattagaga
     3541 aacaaatggc aaaatagttc ctcatatttt gctatttttt gagctaatac cgagggtcag
     3601 ctgattgaac accgtttagt ggagaagttg tgaaaaagtc gcctactttc gagtcgtaag
     3661 aaattttttt gaaaatggtt cctcacaact ttgcataaat ttgacttaat accgagaagt
     3721 catgatcccc atctaactcc aaatcacaag gaatgagttc aaaaactagc ttttctcaac
     3781 aaatattaca taaaaaattc tatgtttttt gttaaattta tttgcttttt tcaaaagtat
     3841 gttacagtaa tttcccaagg aggttttaca gaaatgaatt ttgttgccat ggattttgaa
     3901 actgccaatc atcagcctta tagtgcttgt tctcttgcgc tagtcatggt aaaaaatagc
     3961 cagatcgttg atgaattcta tacattgata cagcctgaga ctcctttttt ctggcgaaat
     4021 gtccaaattc acggaatcca tcaagaagac gtacgcaatg cgccgaaatt cccagaagtc
     4081 tggcaaacta tccaaccata ttttcaaaaa aatcgtttag tcgttgcaca taatgctgct
     4141 tttgatacaa aggtcttagc gggatgctta gattattatc atcttgaaca gcctaattat
     4201 ctttctttat gtacagtgaa aagtagccgt cgcctgtttc ctgaatttcc aaatcatcga
     4261 ttgaacactg tgtgcgaaaa tttgaacatc caattagatc atcaccatga tgcattggaa
     4321 gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa aaaaattcgg gacagatcca
     4381 ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg aaacacacaa agaggtcgtg
     4441 agcctgccgt ttcactccga gtcacaagga gcaatttcaa aaaatagctt ctcatgttta
     4501 ttaaatattg tgactggtga ccgaggagct ggcaggagaa caccgtttat tggagaggtt
     4561 gtgaaaaagc tgttctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
     4621 ttttcatttg gttggactta ttaccgcaga tgcaagcttt tggacaccgt ttacc
//
LOCUS       DAGCWU010000169         4614 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.173, whole genome shotgun sequence.
ACCESSION   DAGCWU010000169 DAGCWU010000000
VERSION     DAGCWU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.173"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(134..1324)
                     /locus_tag="MBZ71_002461"
     CDS             complement(134..1324)
                     /locus_tag="MBZ71_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4430362.1"
                     /translation="MNYFTTSLMKNLFSNDNNLPAFEQAVTELFRSELEKSLNEILAY
                     ELTDFLDYEPYARTNNENSRNGSYQRKFDTKYGTITLNIPRDRMGEFFTSLLPKYRRR
                     DLFTETTILDLFEEGMSHSEICSMIQKLCGARYSKQTISNITDKALECVDSFKNRILN
                     KEYAVVYLDGTSMPLRRDTVSREMVHIALGITVEGTKEILGYMIAPNESAEIWEELLK
                     DLKSRGVERISLFCTDGLSGMENVIQDIFPGSKIQRCLVHIQRNICAKVRVSDRKEVA
                     NEFKEVYRSKSKEEALTSLKKFIDRWKHKYPSMVKSLSNNDYLFTFYDYPESVRHTIY
                     STNLIEGNNKQLKRSFKKKEQFPTEKSEEKYLVIQFNRYNEKNMNHVHRGFGQTTRED
                     WFKS"
     gene            complement(1723..3024)
                     /locus_tag="MBZ71_002462"
     CDS             complement(1723..3024)
                     /locus_tag="MBZ71_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4430363.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
     gene            complement(3036..3653)
                     /locus_tag="MBZ71_002463"
     CDS             complement(3036..3653)
                     /locus_tag="MBZ71_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430364.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPWQV"
     gene            3694..4386
                     /locus_tag="MBZ71_002464"
     CDS             3694..4386
                     /locus_tag="MBZ71_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4430365.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
BASE COUNT         1422 a          832 c          832 g         1528 t
ORIGIN      
        1 tctttcaaaa aattattcaa aaccatatat tgtatttttt atttgataat aacaatggga
       61 ttattaagaa gtggcactaa ataaaaggga gcgtcaagaa ttatgtgtaa aagtatatcc
      121 ttggtaaagc agattagctc ttgaaccaat cttctctggt tgtttgtcca aaacctctat
      181 gtacgtgatt catgtttttc tcgttgtaac gattgaactg aatcacaagg tatttctctt
      241 cagatttttc cgttggaaat tgttctttct ttttgaaact tcgttttaat tgtttattgt
      301 ttccttcaat cagatttgtt gaataaatcg tatgcctaac actttctgga taatcataga
      361 atgtaaacag atagtcattg ttgctgagac ttttcaccat tgatggatat ttgtgtttcc
      421 acctatcgat gaatttcttt agagaagtta aagcttcttc ttttgatttg gaccgatata
      481 cctctttaaa ttcattggcc acttcttttc tgtcactgac tcttactttt gcacatatat
      541 ttctttgaat atggaccagg catctttgaa ttttagagcc cgggaaaatg tcctgtatta
      601 cattttccat cccagacaat ccgtctgtac aaaataaaga aatacgttca acccctcttg
      661 atttaaggtc tttcagaagt tcttcccata tctcagcgga ctcgtttggt gcaatcatat
      721 agccaagaat ttcttttgta ccttctactg tgattcctag tgctatatgg accatttccc
      781 tggatacagt atcacggcgt aaaggcatgg atgttccatc aagatataca acggcatact
      841 ctttgttcaa tattctgttt ttgaaggaat ctacacattc cagagctttg tccgtaatat
      901 tagatatggt ctgtttagaa tagcgagctc cacataattt ttggatcatg gaacaaattt
      961 ctgaatggct cattccttct tcaaataaat ccaaaatcgt tgtttcggta aatagatctc
     1021 gtcttctgta tttaggcaga agagaagtga aaaattctcc cattctatct cttggaatat
     1081 tgagtgtaat agttccatat tttgtatcaa actttctttg ataggaacca tttctagaat
     1141 tttcgttatt ggttctagcg tatggttcat aatcaagaaa atccgtcaac tcatatgcga
     1201 gtatttcatt gagagatttc tccaactctg aacgaaacag ttcggttaca gcctgttcaa
     1261 aagctggaag attgttgtca ttagaaaata ggtttttcat taaagatgta gtaaaatagt
     1321 tcatgggtat attcctttcg taaattgttt ttggtcgaac taattttact aaggatatac
     1381 ctttttttct acatttctag aaaaacgaat ttacacaaga tattttatgc tatccattta
     1441 tttttgaaac tccatttgtt ttactacgtg tattaccttt atttttcttt ctatttttag
     1501 gtgtctatta tctcttacgt tcgcttataa atgatgactc aaaatttaaa gcaaaataaa
     1561 aataaaggaa gtagcctttt gaacaagggc gcttccttta tttgttgtgg gacaccagag
     1621 tatacttttt tataaaaaat aggttgatgg gaaaccctaa gatttcccat caacactaaa
     1681 tagtatagaa ccatattttc gttacaaggt taagaatgac gactatagtt tttcatctac
     1741 taattttgtt ttattgctct ccatgacttt aaaaaatgga tagtagatga agatgctgat
     1801 gacgatcaac agtaggcaaa agagaatatt gcgccagtcc atacttgaaa gataagcttg
     1861 tgcaaaaaaa ggtgtgaagg aaggatcgac gataaatcca atcccaatcc atcctagttg
     1921 ctgagcaaag taggtcagca ataatgaaat gatgggcgta atcaaaaatg gaattgccaa
     1981 aatagggtta aaaacgatag gtaatccgaa aatcactggt tcgttgatgg aacagattcc
     2041 aggaaccaat gacaattttc caattgtgcg atattcgggt actttgcttc gcatcatcaa
     2101 taaaaccaaa cccaaagtac caccggctcc accaagtaca gaaatacgga acatttgtaa
     2161 gttcattaag tgtgtcatgg tttcaccatt tgccagttgt tcagcattca aaccagtttg
     2221 agccattccc aatgtaaaga cgatagggaa aataattgac gagccattga taccaaatag
     2281 ccataggatg ttacccaaag tcacgatcaa taagaaacca cctagactgc cagcgatgtt
     2341 cgttgctgga gtcaggactt ccatcaccgc ttcagggaag attttgtttg ttgctgccaa
     2401 gaaaatcaga ttgatgccat aaaagatgaa tatatttgtc agcagtggaa cgagcatatt
     2461 gataaaggtt gctaccatgg gtggaactgt atctgggagt ttcaacttaa tattttttac
     2521 ttcaaatagt cggttgattt ccactactag caagccgatg ataattgcga cgaacaatcc
     2581 gtttgtccca aggaaattaa gattgatggc accttcttcc actggtgtac aaaccattag
     2641 ataaatgaca gatgcaacca tgccattcat ggaaggatta attttgtatt cattggcaag
     2701 ggaaaatgca atgccaaaaa cactaatgaa tccgatgatt cccattgtca aattataagg
     2761 ggctgtgatg agatcataat ttgctacggc aaaatctttc cagctggcca taaatttcat
     2821 gaaaatatta gctgtatctg gattgtattt atcaatattg atgggtggat ttgcaaaaat
     2881 cagaaaaaaa gaaccgataa caataaaggg taggccaaac atcataccgc tggatatagc
     2941 ttttaggtgg cgctgattac ctatcttggc tgcgatgggg ctaagtatgt cattcagttt
     3001 catgataaac gcagaatttt ccatataact tcctcctata cttgccaagg attaaatgaa
     3061 aacgtacttg gcacgacgtg atctggatca taggtctcga tgattttttc gtattgttcc
     3121 ttgtagtcat tatcaacttt tgcttggggg atcactttac ttgcttcatg caaaaagtgt
     3181 tctgaaccac gccccattgc ctgtccccga acagtatgtt tatccaacgc aatatcagga
     3241 atttctggaa tatatcccat agcgaaactc ttgataacaa tgttcttaag taaatccgat
     3301 gaacgatctt tttcagattg gcataggtaa cgaatcgcgt ggaaaaaata catcgcacga
     3361 tcggattcat tgtattggaa ttctttgcgc atggcgttta agttattgat catgatggcg
     3421 gccataggat tgcccatgcc cacatcctca acactgatcg ccaatagcct tctccagagt
     3481 ttctcttcaa attgtggtga ggtgatatac atttcataag caaattcaca tgcttctttt
     3541 tcttttcctc gacgaatcga tttctgtaat gctgagatag cttcatctcc acgtaagccg
     3601 ttacgggtag tcatttttga ccacggatct tgaataaatt tcgtttctgt cataatataa
     3661 tactcctatt agataaaatt tgaggtgaat cttatggatg ttgaaatgct tatagataaa
     3721 taccaactga atgactcaga agctgaagtc ttacgattta tgaaaaaaaa tcgtcagtct
     3781 ttaaaaaata ttgggattcg tgacgttgcc aaagcatcgt atgtttctac tgcaacgatt
     3841 atcaatatgg cgaagaaaat tggatattcc ggttatagtg agttggtatt tgctctagga
     3901 aacagccaat ctgacattac gatgccaagc acattctcca atgaggagaa ggaagcattc
     3961 atacatttaa tgtctgcgta tcgcgataaa cggattatga ttcttggctc aggtttttcg
     4021 caaaatctag ccaattattt ttcggaatct cttaatctat atggatttcg tgcgacggcc
     4081 aatagtcatt tagaatttct tagacagcaa gtgcaacaag atattctgct ttttattatt
     4141 tccaattctg gtgatacagt tcgattagcc gaattagcta aaattgcgaa cgatcacttt
     4201 atcgatgtga tcgcatttgt tgggcaaaaa aattcaaaaa tcggtacgct ggcaacatta
     4261 accattagca cggaaaccta tattccacaa aaaatatctg attaccaacc aaatcttttc
     4321 tttggcacga cactgaatca atttgagctt ttgttgagcg cggcgctgaa ggctatttat
     4381 aaatagaaga acgctgtggc cactgtttct tcaaaatcta gtataaagaa tcttctttag
     4441 aatgtaattg ttttctgtgt gttctgaaca tgtttaggaa tttccaaact tgtttagaaa
     4501 actttctcat tccctccgct gtagaattga actcacataa aaaacatcta aaccaaaata
     4561 tctacaagct tggaaaggat aataggttgt tattaaaaat aatttttatt cctg
//
LOCUS       DAGCWU010000170         4561 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.174, whole genome shotgun sequence.
ACCESSION   DAGCWU010000170 DAGCWU010000000
VERSION     DAGCWU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.174"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(715..831)
                     /locus_tag="MBZ71_002465"
     CDS             complement(715..831)
                     /locus_tag="MBZ71_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBT4430366.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(1307..2185)
                     /locus_tag="MBZ71_002466"
     CDS             complement(1307..2185)
                     /locus_tag="MBZ71_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBT4430367.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            complement(2508..3533)
                     /locus_tag="MBZ71_002467"
     CDS             complement(2508..3533)
                     /locus_tag="MBZ71_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4430368.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            3892..4395
                     /locus_tag="MBZ71_002468"
     CDS             3892..4395
                     /locus_tag="MBZ71_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBT4430369.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
BASE COUNT         1501 a          829 c          729 g         1502 t
ORIGIN      
        1 ttgtaatagt gattctcaca aaatttcatg aaaaatactt tattactgta tctaaaattc
       61 caatacacaa cttatcgact attatctgaa aaccaaaact tattattcct tgatgttatt
      121 ctagcaatag taactgcttt tttatttttt tgcatatttt ttcggcgtta ctttttaaag
      181 ttaacttttt cgacaaatag aaagagatac gaatcactga tttttttgaa gaaaagaaat
      241 ggagtgagct gaacgttaga gatattggga gctttcgtaa ctgcttatta aacgataatg
      301 gttatttgca accgtattgc tcgcttctac atgaaatatt tcatgaaata tttcgcaaaa
      361 cctttgatta tgtagttacg tttccattct tgactaaaaa tgtttcaaaa acgaacaaag
      421 gtgttaaaag gtaaagtgat caatactact ggtcaaaata agaggttgaa agggaattga
      481 aaaataacta cagataatac ttttataaac gtacgtgctt tgtggttctg tagttaacta
      541 tataactgga attagggaat ggtatcaatt tgaaaagaaa gattctacca atttatcata
      601 tgttaaaaat ggaaaatcaa cagcagttct ttcggaaacc ttacacaaaa accgattatt
      661 gaaaatagcc gaaaaacgtt tttcggctat ttttttaatt aagtttcctt cttcttacct
      721 tttcccacta ttccgtttca catctttgat gatcttcagc gcacgttttc gtgttttgat
      781 atttgggctt ttcaagcggc ggtatgcact tgagatatct gttttttcca tatggacgac
      841 ccactttctt ctttgattgt agtcatgcta aaaaacacaa atcgaaacaa ttacgatttg
      901 tgttttaaat ggtatcaaaa gttaaaatga aagtcaactt gttaaaacag tcgatcagac
      961 tagctgattg actcctatct ctggaaataa attttcatta ttacgtttta gctacctaaa
     1021 aaaggccctc ttgtccgtta actttaatat ctattgttat agctagttta cctattgaac
     1081 gtactgtgtg ttcgatagaa cactaaaaaa gaggctggga cagaagcgtt taactccaag
     1141 aaataagaag aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc
     1201 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     1261 tttacttttg tctcacgctc ttttaaacta tttcgaagta ctaaacttat gaagtaagaa
     1321 tacctaagcc agagccaatg tgctcgcctg caagcaattg ctcttttgct tgtcgctgta
     1381 aactcgtact ttctggataa tcatgtattt gggcatctac tcgtccaaag atccaaggga
     1441 ctccactatc gtcctgttca tcaactgata ataatcgata atcggctgca aaaagacaga
     1501 tttttgactc ttttttcaac catttagtaa gattgggtga tagtaaccaa gtatctgtgt
     1561 acatcacttg gtaatggtaa tcaggaaaat aaagttcaaa aaaacgaatg gcactttcgt
     1621 aactatctct tatcttattc tgagataaat ctgcatcgga aggaatatga agagaaaggc
     1681 actgctttcc ttctaatctt agatccgtgt accctttcgg aaaatatgtc atttcgtact
     1741 ccaattcacc aatccgaaag atcaagccac tagtgtaatg ccaaatccaa aaaccacggt
     1801 caaagcgata gcttcctgtt acttggaaat gttcatgaat aaatctagta aaagcattca
     1861 ttgttgccaa atagatagtt cttggaatct tcaatggttg ataaattttt ttccaacttt
     1921 gagaagctgc atatagataa atagctaatg ctgcaaaagg ttcctcttca tagactttgt
     1981 ctattttctt tgcagtcgcc tttgctttat taggatccat tagttcatga ctatattgct
     2041 caaacacctc atcaaggatg ttttttgctt gtacaatcat ttgctctata acgactttgg
     2101 gaagagttaa agtttctgcc agaatttgga tttcattgct tattttcttc atcaaatttc
     2161 cccttttcat atctttgaac ctcattgttt aaatgactga ttatccggac accatatggt
     2221 ataaacatac ttttattgta cactaactat aaaacttaaa ctttctatga agaaagaata
     2281 tctgtaagac tttcaaggaa agcattgacc atctcaggaa agtttattca gttaccatca
     2341 taaacaaaat tttttgatag aataaatata aaattttttt gaaagttcct ttccacattt
     2401 ttgtgtgaat ttgacttaat gctgagaatc tggcttttga acaccttgaa ttagaggttg
     2461 tgacaaaaat cttgtcataa cctcttttgg cacatatatt tgtccttcta ttctaatttt
     2521 tgacgaacaa ccgttaaata atgttgcaaa ccaaccgtca ttacccacgc aacacctaaa
     2581 ccgatgaaac ttcctatgac gatccctaaa aaacgatatt cgatcaaaga aatcaagtat
     2641 gctccacgaa taagtgtaga tagcatcaac gccatcggat taagaaaaat attagcaact
     2701 gcataatttc tagcaactag aaattgtgcg attccatata gaaaagtgat cagaaaaatg
     2761 gtctgcaact gcgtaaacgg cacaaatgac aagaatgccg acacacataa cccacccatc
     2821 gatccaatca gatattggac ttgtcgatgc ttcatcgcat ccaagttttc tgctagcaaa
     2881 atcgatgcac atgacaaggt caaccaataa ggattatgca gattcaagcc gtgactgaca
     2941 taaacggaaa gaaataaggc agcgctgtag aaaacagaat caatgatcac taaaggctct
     3001 tcgtgaaatc tttcttttaa agttgctttt tcaattgtct gctcaggccg tgaatccagt
     3061 aattttgtaa tcactgccgc aatcagggca aaaacgacac ccataaaaaa ataaagtgag
     3121 acgatcggta attgagcgat tggcagctgc atgctcgttc ccatggcaga aagcatcgca
     3181 aaaaacaagc cacctggttt agaaatatgg aataatcgca acacgaatct gctggaaaaa
     3241 gcaaccaaag ctacggcaaa tggttcaagc cataaaacat aagtcgataa gatccccaaa
     3301 acgaaagcaa ccaacagtcc gcaaccgatc agaaacatac gtttcagcaa atatttcatc
     3361 ggtatgtttt gataatagaa aaaagtaaag atccctaatg atgaaaaact tgaaatcaaa
     3421 aaatcatgat ttatgtaacc taaccataat acaataaaga taagtagcat cagattcatg
     3481 atacgaaaag gattatcttg tattttattg aaacgaaaat aagtcttgaa caatctatcc
     3541 ctccttccta gtaattggaa aaaaagctga accaagatga aatgccaatc ccgtttgtca
     3601 gcattaccta gtataacaac tatttcttga tttttcataa acttagtatc aaaaagtaaa
     3661 caacttggag aaattagaac aaaaaacgaa cattccgagt cgttttgaca gaaaaaagat
     3721 agcaactttt ccaagcaact cgctatctta gctttgttaa agaaaccgga ttcccaacaa
     3781 aaaaaagctt ctaattttcg tacgataact acgttggtcc ttaaacttca tactttttta
     3841 tgatttttca gcggttttcg atggtatact ttttaaatgg aggagatgtc tatgcggtat
     3901 gtatcaaaat ttttattagg gatttattta ttctttctat tatggttgat tttattcaag
     3961 ctatcattta atcttccaca atttctcact tattcttaca gcaatgtcaa tctcgtccct
     4021 ttttctactt tttcttttga aaatacgacg gtattacgtg agaccactta taacttgatt
     4081 gtcttttttc cttttggggt gcttttgaat gtcaatttca agagactttc tttttctaaa
     4141 aaattaggga ttattttcct tgtaagcttc ttggcagaat tgattcaatt cctattcggt
     4201 attggcgtag cggacatcac tgatttgata actaatacaa caggaggact gataggttta
     4261 tgggcctatc aacttctaaa caaacatcta tcgacaaata agttagaccg tctagccatt
     4321 attttgggat acattttatt cttatttctg atcgtagctt ttttattttt gacccgaact
     4381 gtccgtcttc aataaaatcg ttcgtaaaaa aagaccgtaa tagacacatt ccaatgtgta
     4441 tctatgacgg tctttttatt cagtcattgg tttaaaaaaa tctattttca tctctaatct
     4501 actatattat tgcagcagga cgacttgcag tttgaaactg acaggctata ttccccttat
     4561 t
//
LOCUS       DAGCWU010000171         4464 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.176, whole genome shotgun sequence.
ACCESSION   DAGCWU010000171 DAGCWU010000000
VERSION     DAGCWU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.176"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            573..1007
                     /locus_tag="MBZ71_002469"
     CDS             573..1007
                     /locus_tag="MBZ71_002469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430370.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2297..3220
                     /locus_tag="MBZ71_002470"
     CDS             2297..3220
                     /locus_tag="MBZ71_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4430371.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3236..3775
                     /locus_tag="MBZ71_002471"
     CDS             3236..3775
                     /locus_tag="MBZ71_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBT4430372.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          667 c          756 g         1374 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcacgcgctt
      241 atgaagcgct accaaaaatt taccatattt tacaaaaaat aacaagtctt aaaaaaatga
      301 cagataaaaa tgcacaaaga attagatata gataaccgcg aaaaatcaag ctttagaaag
      361 ttgcttttag ctagaccaat taaaaatcac aaagtgagcg tctctatcca ttggtagagg
      421 ggctacttgg catagtgtac attatgtcta ctaaccttta aaataacacg ttttataaaa
      481 taaaccctct tgacaatatt taattttctg attattctaa atatatacag aaagcgcttt
      541 cctaggattt tatatctaag gaggaagtaa caatgaaaaa taaaattatt aaaattatta
      601 cttgtctatt tttgttcggt atgattgttg gagcaagtac tccgttaaat ctgtttcctt
      661 ttagtgctag aggtataaaa gtggaagctg caagttcacg atataatcat aatcatcgtg
      721 gatttacttg ctataaaatg aactattatg ttactaataa aatgtgtaga gatttgaaaa
      781 aaaattataa taagctaaag actcctgctc aaattgcatc ttttattcct attggtggag
      841 ttggaacatg ggttattaca aatgctttta gtagtgctgt tgatgcaatg aatgtctttg
      901 taagagctgc aaatcaagga aaaggtgtac aattaacata taatgctcac tttagcaata
      961 caacatcata tcaatacaat gattacgctc gttatgtaat aaaataatca gaaagtgaaa
     1021 ctatctcatg aataaaaaga ttttagctgt aattatttca ataatagttt taatcactat
     1081 gttcttcata tttaggctaa ttttcaatat aagcttacaa aaaagtattc tttatctaat
     1141 tcctatagca tttatcttag caatttttag aagtatttac ggaaataaaa actctaaata
     1201 gctaaaaata aatcatacta aattttacaa aaagtggcta gcattttcta gccacttttt
     1261 gtattgtgga gcgtgggttt tgagggggat tcaaggggga gcttgcgccc ctttgacaag
     1321 cggttatttg caccaaatac ggaggtattt ggctagcaaa tatagagctt gccaaaccct
     1381 tttagtgagt tttgtagaaa gtacctttaa aggtactttc acattacgaa cgatacgtcc
     1441 gtaggacgta ctgaaagggt gggacttatg agcaaattaa tgcaaaattt ggctagcaaa
     1501 aaagctaaaa aatatatgct aataatcgag cgaaataaac actttttcaa gtgagcgaat
     1561 ccgccatgac aaagctgaag cagtttattt cagctttgtc atggcgtgtg ccgacttttt
     1621 taaaagtcta ggcacacgag caaatatctt tatcaagctc taatttgcgt tttaagacgt
     1681 ttgaaacgcc taagtatata aatatataag aataggttta agagtgctta aatcgaattt
     1741 tagagggcat ttagacaatt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgt
     1801 tttaagaaca agataaggga tttatactag gactgtctta aaatgtctta aacgaacgct
     1861 cagagcttct caaagggttc cttatgaacg gcttttagca aaggagccgg aggcgactgc
     1921 gcagtataat gtgagagcag agcgatcaca cctttttagc gaaccgtagg gagcaaggtt
     1981 tggaattggc agttttgata aattcgcgaa cctgtttaga gaaaaaagga gtgatttttt
     2041 aggcgggaaa attagcctcc cctcttttta tatctctttt tatatctctt tttatatctc
     2101 ttttatgtcg gctgtgtcct tactctccca agcaattcag agttttttca agtaggtttt
     2161 ctacgctttt caagtaggtt ttctacgctt ttcaagtagg ttttctacgc ttttcaagta
     2221 ggttttccga cattattgag tatttttata aagtatgtta cttttatttc atactttata
     2281 aaagtaggag aagcttatgg caaatgaaat tgtaaaatat gaaaatagat taaatcatat
     2341 tcctctgaga aaatttaata gtcgtgaaat gaacttattt ttttcaattg cttcacgtgt
     2401 acgtgaaaaa ggtgtagatg agattatttt ttcatttcac gatttgaaaa aactaagcgg
     2461 ttatacagaa cgtggggaac aatttataaa agatcttagt agcacgtatg acaaattatt
     2521 aagtattaat gcttggaatg atgatggaaa tactttaact aaatttgttg ctttcacaaa
     2581 atatagcatt atacgagata aacaacttgt tataatttca gttaatccag aatttaaagg
     2641 actttttaat caattaaaga actggacaag atttaatcta gagcaattta ctaatttgga
     2701 tagcacatat tcaaaaacta tgtttcgatt attaaaacaa tatcgaactc aaggctggac
     2761 tgaattttcc aaagaaaaat tttttgattt actagatatt cctaaaagtt attggaatag
     2821 tccgtcaaat attgacaaat ttatcttaag gccaattaaa gaagaactca ctcccttatt
     2881 taaaggatta actattcgta aaaaatatgg taaaggtcga ggtaaacctg ttatcggtta
     2941 tcgttttact tggaaagctg aaattaacca tgctgacgat ttttccaaag ggaaacaaga
     3001 agatttacgt ataaaattat ttaatattga tcacaatgga gaattaacac gagaagaaaa
     3061 atggcgcgct aaagatcgaa tactaaatct tcccttaggc actcatgaag ctgattttaa
     3121 acaacaacaa caaactgaag aaaaacaggc agaaaaacga gaactttcaa acaaactcaa
     3181 acaagattta ctaggaaatt tacaaaactt attcgattaa gcttaaggag tatttatggc
     3241 agataaaaca atcaaagaac ttgcggaaga attaggagtt agtaaaacag caattaacaa
     3301 aaaagtttct gacaaaaatc gaaaactttg gtttgcaaaa aacggaaaca agtttgtgat
     3361 taacgaaacc ggtcaaaaag ctataaaatc aatgttttta gtcgataacg caaacttaaa
     3421 tcgaaaacta gttggcgaaa aatcaaaaac caaaaaccat aaaaacacca accaagtttc
     3481 cgaattaaaa ctcctttctt tcctagaaaa acagttaaat cgaaaagata tacagctaca
     3541 agaaaaagat aatcaaataa aacaaatgca aaaattactc gaccagcaac aacaactcac
     3601 tctacaagcg aaccaacaaa ttgagaacct acaagaacag ctccatttaa cttatacaga
     3661 agaaactcca acaaccgaat caactgcctt ttctgaaaaa aaagcagaca atacacaacc
     3721 acttattgaa aaaaagtggt ggcaaatttg gaaatctaat agaaccaatc tctaaaattt
     3781 tctagaagtt agttcggata gtagaggaaa aagttgtact acctgatggg gagatcactc
     3841 aaatttttat gagaaagaaa aaatagttta caactgtaaa caatcaatgt ttatagttgt
     3901 aaactatcaa actatatggt gacttattgc ttaaaatagg ttgaaatctc actcaatttt
     3961 tggtggaaaa acaaaataaa ttctaatcga ttttgttgaa aaagtcataa aaaaacaaat
     4021 gtgattttaa caaaatttca aaaaaatcgt ttgaaatttc gtttattttt tttgagaatt
     4081 ttgatcatcg tagggtcatt gtagaatcat tatgagatca tcgtgggatc atcgaataaa
     4141 gtatgggaaa ggcttgcggt gcttggtatt aagggcattc tccgcctccc aggttcttga
     4201 ccattttctc tcaacgtggt aaaaatccca cttataagag aaaatggtca agaacctaaa
     4261 cggtcggcga agaaaacata ccaagcacca caagcaaaaa aagttagagt aacttccaaa
     4321 aaagaaaaat tttttagaac aacttttgaa aatttaattt tgatttatat ccgttcaatt
     4381 taaacatttg ataaactgaa ggctagtatt taaaatgtgt atacaagtgg tagcgttaaa
     4441 aaaagtggag gtataaccta tgtt
//
LOCUS       DAGCWU010000172         4373 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.177, whole genome shotgun sequence.
ACCESSION   DAGCWU010000172 DAGCWU010000000
VERSION     DAGCWU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.177"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            367..924
                     /locus_tag="MBZ71_002472"
                     /pseudo
     CDS             367..924
                     /locus_tag="MBZ71_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            982..3021
                     /locus_tag="MBZ71_002473"
     CDS             982..3021
                     /locus_tag="MBZ71_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBT4430373.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3130..4281)
                     /locus_tag="MBZ71_002474"
     CDS             complement(3130..4281)
                     /locus_tag="MBZ71_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBT4430374.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1585 a          808 c          859 g         1121 t
ORIGIN      
        1 cattgcctaa tcattaggaa aagtctatca tgaactcgta aatagaatat agtaataacc
       61 ttgttaggtg aggctcctat acaaacatag gctgttgccc agaaacgtcg aaagtcgcca
      121 atgggtaaaa cagaaactgt tggattaagg ctttttccaa tagctaagaa tgttttcttt
      181 acgttgtata gtgctaaaac tcggacgatg agatgatgct aggtcagaat tcttgttcga
      241 aaaggtttct gaccttttct ttatattttt gtgactgact tatttgatga gacaaactcg
      301 ctcctcacct gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt
      361 ggtggaggtc gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca
      421 aaactgatct ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag
      481 ataaatgcag cttactctta tgggggagtt gatttattaa atcaaacatt aaaggaaaat
      541 ttcaaatttg aagctcccta ttatgcaagt atcacatttc aagattttat cgattgcgtt
      601 aatgaactgt ttcctggtgg agtcaagatc gatgcagaaa aatctctaga tttagatggc
      661 gtatatataa acaaaggaga gcaagtgatg gatggtaata ctttactcca gtatgctcga
      721 tttcgggaag acaaagaagg agacttcggg aggatcagaa ggcagcagca agtgatcaaa
      781 gctatctctc agcagctgaa agacgtaact tcaatattca agctacccaa agcggttggg
      841 aaattacttg gtagtatcca aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg
      901 gatttcctca aagacatatg ttagaataaa caggtataaa tagtgaaata aaggaatgac
      961 aagcaagaga aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc
     1021 gcttatattg ccggcgcagt gatcttaata gcagctgcgg gtggcggtta tttttactac
     1081 cagcactatc aagaaaccca agcagtagaa gctggagaaa agacagttga gcaatttgtc
     1141 caagctttga acaagggaga ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca
     1201 aataaaagtg cattatctga aaaagagatc ttagataaat accagaatat atacggtgct
     1261 gctgatgtca aaggacttca gatatcgaat ctaaaagtag ataaaaaaga cgattctact
     1321 tacagttttt catataaagc aaagatgaat acctcattag gtgaattgaa agatctttct
     1381 tataaaggaa cattagacag aaatgatggt caaaccacga tcaactggca acccaacttg
     1441 gtttttccag aaatggaagg aaatgacaaa gtaagcctga ccacacaaga agcagcaaga
     1501 gggaatatta tagatcgaaa tggtgaacca ttagcaacaa caggcaaact aaaacaatta
     1561 ggagtcgttc caagtaaact tggagatgga ggcgaaaaaa cagccaatat caaagcgatt
     1621 gcttcctcct tcgacttaac agaagatgct atcaatcagg cgatttcaca aagctgggta
     1681 caacccgatt actttgtccc attgaaaatc attgatggag caacgccaga acttccagct
     1741 ggagctacca ttcaagaagt agacggcaga tattatcctt tgggtgaagc agctgctcag
     1801 ctgattggtt acgtgggaga tatcacagca gaagatattg ataagaatcc agaattaagc
     1861 agtaatggaa aaatcggtcg ctcaggtttg gagatggctt ttgataagga tcttcgtggg
     1921 acaacaggtg ggaaattaag cattacagat gcagacggtg tagagaaaaa agttctgatc
     1981 gaacatgaag ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa
     2041 atagcttttg acagtctagg aggaaaagct gcatcaactg ttgcgacaac gccaaaaacc
     2101 ggtgatcttc ttgcgcttgc tagctctcca agctatgatc caaacaaaat gacaaacggg
     2161 atctcacaag aagattacaa agcttatgaa gaaaatcctg aacatccatt catcagccga
     2221 tttgcgacag gttatgctcc tggatctacg tttaaaatga ttacagcagc aatcggtctc
     2281 gacaacggca ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa
     2341 gacagttctt ggggatcttt taaaacaacg cgtgtcagta gtgatgtatc acaagtagac
     2401 ttaaaaactg ctttgatcta ttccgataat atatatgcgg cacaagaaac gttgaaaatg
     2461 ggtgagaaaa aatttcgtac aggcttagat aaattcattt ttggtgaaga ccttgatttg
     2521 ccaattagta tgaatccagc acaaatttct aatgaagata gctttaactc agatatcttg
     2581 ctagctgata ctggatatgg acagggcgaa cttctaatta ctcctatcca gcaagcagca
     2641 atgtattctg tttttaccaa caatggcaca cttgtctatc ctaaattgat tgcagataaa
     2701 gagacaaaag ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat
     2761 ctgagagaag ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt
     2821 ccgttggcag cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa
     2881 gagaacagtt tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg
     2941 ttggaaaata aagaagatga tgattcagca accaaacgag catcggaact attgcaatac
     3001 ctcaaccaaa attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt
     3061 atcgtcacag tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt
     3121 ttgatttact cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat
     3181 aagtgtactt aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg
     3241 actttgctgt ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc
     3301 accgaccatc aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc
     3361 aagcaatttg gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact
     3421 aaagtcagaa aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc
     3481 atatcctaaa gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc
     3541 tatcccagca aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga
     3601 cattcctaaa caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc
     3661 tgcaaatgaa agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata
     3721 aattagctgt tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag
     3781 aaaagatagg ctgaaactta gaactaaaac gactaaaatg tcatctacca cagaagcacc
     3841 caaaattgtt gatccttctt ttgtattcac tacgtttagt tcttttaata cttctactga
     3901 aatactgaca gaagttgccg caagaataat cccaaagaaa atcgcttcat ttcccgaaac
     3961 ttggaatgct ttgcctgtca accagccaaa aacaaccggg aagatgatcc caagcaaagc
     4021 aacgaacata ccaggtcgga aatatttttt caaaagagat aaatcacttt ccagaccagc
     4081 taaaaacatt aataagatca caccaatctc cgaaaagtca tggactagaa tatcaggttt
     4141 gacccaattc aatccagctt ggcctagcaa aacaccaaca agtaattgtc caatcactgc
     4201 tgggatacct aatctccgcg agatatgtga gccaatcgtt gttgaaatca atatcaggca
     4261 taaaatacca ataaattcca ttctcatcat tcctttgtat aaaatatagc ccgaacttag
     4321 cataacatac attattgaaa aaataggaaa atatactcac aaaaaaacca aca
//
LOCUS       DAGCWU010000173         4364 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.178, whole genome shotgun sequence.
ACCESSION   DAGCWU010000173 DAGCWU010000000
VERSION     DAGCWU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.178"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            77..547
                     /locus_tag="MBZ71_002475"
     CDS             77..547
                     /locus_tag="MBZ71_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBT4430375.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            632..1240
                     /locus_tag="MBZ71_002476"
     CDS             632..1240
                     /locus_tag="MBZ71_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBT4430376.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1374..1574
                     /locus_tag="MBZ71_002477"
     CDS             1374..1574
                     /locus_tag="MBZ71_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4430377.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            2328..3554
                     /locus_tag="MBZ71_002478"
     CDS             2328..3554
                     /locus_tag="MBZ71_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBT4430378.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(3662..4132)
                     /locus_tag="MBZ71_002479"
     CDS             complement(3662..4132)
                     /locus_tag="MBZ71_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBT4430379.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         1522 a          640 c          859 g         1343 t
ORIGIN      
        1 tttttttaaa taaagatgaa agttattgaa atatagagta tgataaataa gaatttttaa
       61 taaggtaggt tggctcatga agcagtatcg acagttaaaa ggatatttag aagcaaaagg
      121 atattgggag atactagtct atctcttttt tggtggtttg gctacgattg taaattttgt
      181 tagttttgct ctcgcacgcc aatattttga tttaagtatg gccttatcca attctatttc
      241 gtggttttgt tctgtattgt ttgcttttgt aacgaacaaa ctttgggtat tccattcaaa
      301 atcaccgaat tttacacatg cacttattga atgcggtaag ttcttcttct accgtatcct
      361 ttcttacgga ttggacatgg gggcaatggt attgttgatc aatgttatga acagcaatga
      421 gtatgtggcg aaaatcatca ctcaaattat tgttatccta gcgaactata ttttcagcaa
      481 gttgtttatt tttaaagaaa ctgaggtttt tgaagaagaa gtaggtcaaa gtgaaaaaga
      541 cgactaggca taacaattga agaatttcct tctctttgtt actctttgta atgtagacat
      601 tataaagaaa acattgagga ggaatttttt tatgacttac acattaccag atctacctta
      661 tgcatatgac gcattggaac cttatattga cgaagaaacg atgcatctgc atcatgataa
      721 gcatcacaat acttatgtga cgaatttaaa tgcagcaatt gagaaatacc cagaattagg
      781 cgaaaaaaca atagaagaat tattatctga tatggacgct attccaacag atatcaagac
      841 agctgtacgt aacaatggtg gcggacatgc taaccattca tttttctggg aaatcatggc
      901 accaaatgct ggtggcgaac ctacaggaga aataaaagaa gcgattaatg aagcttttgg
      961 tgatttttct tcttttaaag aagaattcaa aaaagcagcc gctggacgat ttggttctgg
     1021 atgggcttgg cttgtaatgg aaaatggaaa attagctatt acctctactg caaatcaaga
     1081 ttctccattg atggaaggaa agacaccaat tctaggtttg gacgtttggg aacatgctta
     1141 ttatttgaaa tataaaaatg tccgaccaga ttatattgca gcattctgga atgttattaa
     1201 ttgggatgaa gtaaacaaac gtttcgaagc agcaaaataa ccatttcctc cgtcatatca
     1261 gtcttaaaaa taatttggaa aataaaagag cgaaagcttg ctagcgtttt ctaaattggg
     1321 tataatgttc ttgcaaggtt acttgcaaga acttttttgg aggctataca tttatggaac
     1381 acggaacggt taaatggttc aacaatgaaa aaggatttgg gtttattaca gtagacggcg
     1441 gagatgacgt atttgttcat ttctcagcaa ttcaaggaga cggcttcaag agcctagaag
     1501 aaggtcaaga agtagaattc acgattgttg aaggtgcacg cggacctcaa gctgctgaag
     1561 tgactaaatt ataataattt gttgaataaa gacacgactt cttagagagt cgtgtctttt
     1621 tgtctattta tgaagttttt agattttttg gctaagtaaa tgcatttgtt cacttgacat
     1681 gtcctaacta attatcaagc aaggaacatt ttcttcgtta gtaatggatt ttaaagaaat
     1741 ttcagttttt ggatgttgaa taaaagacca tttgtgaaac cactagtata catactcatt
     1801 accatgtcaa ttctattact ttaaaagaaa atcgttttac tttgaagagg agcatatcta
     1861 tccattaaaa gatgatggta atgaaatcac tagttcgagt cattttaaga gccatgcagt
     1921 gagctggaaa taaaacgaat agaagatggg tttaacaaaa ggaccagtca taggaagaaa
     1981 ttttcgtgaa atcgaatgat aatctgaacg gtaggcaaag actgttaaaa agatgggaaa
     2041 ggtatgtgcg atcattttac tgcacgatta tttggaaaat accttgttgt tcgctttttc
     2101 tggaaaaaat acgtaaaaaa agataaaacg attgcgaact gcgtgtattc ggtctttcga
     2161 tctttttcac ggtatggtat gatagttaag aattttgaga aattttaaaa aatagtaatc
     2221 gttacacaaa aatgtttttt aaagttttct tatgattaaa aagtgaattt gctgattgaa
     2281 cagttatatg agacaatatt ttagaaaatg tgaaacagag gtgtattatg aagcgatggc
     2341 aaaaagttct attaaccttg ttaggggtat ttgttttgct gattgccgga ttatcagcat
     2401 acggaatcag atttatggga gaagcgagcc aaacagtaaa caaaatttct aaaaactcta
     2461 atcgaatatc ctccaaacgt tctgagagag taagcattga tgacaaagaa cctttttcgg
     2521 ttttgctatt aggtttagat accggtggat taggaagaac ggaacaagga cgttctgata
     2581 cgatgatggt agtaacagtt aatccgaaac aaaaaaaatc aacgatcatc agtttggacc
     2641 gagatattta tacgaatatc gtaggttatg gaacggtaga taaattaaat catgcctatg
     2701 catttggtgg tgtcgaaatg gccatggatt caattgagca actgctcgat attcctattg
     2761 atcattatgt aacgatcaat ttggatggca tggaagattt gatcaatgcg gtcggtggca
     2821 tcaaagtgaa taataaaatt gactttactt tggatggtgt ccatgtacca gtaggtgaac
     2881 aaacattgaa tggagaaaaa ggtttagcct attctcgaat gcgacacgaa gatccagaag
     2941 gcgatatcgg tcgacaacgt cgtcagcggg aagtagtgac gaaaatcgtc aataaagttt
     3001 taagtttaga tggcgtaagc aattatagaa aaattttaaa agcagttgag aacaatgtga
     3061 cgacggactt agattgggat gacatgcttg atgtagcgac gaactacacc cctgcgtttg
     3121 aaacaatcaa acaagaccag ttgcaaggcg aaggacaaat gatcaatgat atctactatc
     3181 aaattttagg aaaaaatgat cttctttcga tccaaaatga actgaagaaa caattaaata
     3241 taaaaacaag cgatacatta cctaatttga aaaatgagaa tgcttcaatg atgttctttg
     3301 atgattcgga agatggcgat tcgacaaacg gtgatggatc gaatagtgaa gggtacaccg
     3361 attattcttc ctattcagaa aacacatatg atcagtacca agatccgaat acctattatc
     3421 agcaaacaga agatacaagt cagtactata caccaagtca agatactcaa agttatgatc
     3481 cttacgctgg acaggaacaa aatggttatg gttatgacca gaataatgag tatggaacaa
     3541 caagtgggta ctagaaaaaa atagtttggt taataaagag tgccacctaa attagacaaa
     3601 aaatctaact taggtggcac tttttctatg tgttaaatta caggacgtct ctttttagcg
     3661 cttagtttaa aaaataaatt gaataattca atagagttgc gaatagtacc cataaataat
     3721 aaggaatcat gagccacatt gccaggcgat gatgtagcca caactgatac ataagcactg
     3781 acaagaaaat aactaaaagt gtaatatcga ttgctgcaat gaaaatattt cttaaattaa
     3841 aaaatagagc agaccatata aaattaaaaa tgaactgaat cacaaaaaga ccgacaagat
     3901 tgtttcgctg ttttttgttg ttgtgattaa gtaacaaata aagagaaata cccatcaata
     3961 tatacagtaa ggtccacatc ggcccaaata tccagctagg tggtgcaaat ggtggaagtt
     4021 gcaaagaata gtaatattct ccgggatttc cgctaagcaa ccctgataaa aagcctaata
     4081 tgacaattcc tataacacaa acccaaaaac gaatatcttt tatcatcttc attttactaa
     4141 ctcctttcta taatagtagt ttaccatgaa ggctcacagc aagacctgta tttagtttgt
     4201 gaagtaagaa aaaaattgat gattgttgtt attaaaaagt tgaacatttg tttatatgaa
     4261 tgatgttttt gaaaaagatc atatgatttg gttctcatgt tttctatttg tacttttctc
     4321 gtctacttct gttataatct gcatcttact tttttcacct tttt
//
LOCUS       DAGCWU010000174         4342 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.179, whole genome shotgun sequence.
ACCESSION   DAGCWU010000174 DAGCWU010000000
VERSION     DAGCWU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.179"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            45..1418
                     /gene="tet(L)"
                     /locus_tag="MBZ71_002480"
     CDS             45..1418
                     /gene="tet(L)"
                     /locus_tag="MBZ71_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBT4430380.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            1982..3244
                     /locus_tag="MBZ71_002481"
     CDS             1982..3244
                     /locus_tag="MBZ71_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBT4430381.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3489..>4342
                     /locus_tag="MBZ71_002482"
     CDS             3489..>4342
                     /locus_tag="MBZ71_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBT4430382.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1511 a          589 c          893 g         1349 t
ORIGIN      
        1 tttttaataa agatttgaat taaagtgtaa aggaggaatt aattatgaag tgtaatgaat
       61 gtaacagggt taaatacaac caaattttaa tttggctttg cgttttatct ttttttagcg
      121 tattaaatga aatggttttg aacgtctcat tacctgatat tgcaaatgat tttaataaac
      181 cacctgcgag tacaaactgg gtgaacacag cctttatgtt aaccttttcc attggaacag
      241 ctgtatatgg aaagctatct gatcaattag gcatcaaaag gttactccta tttggaatta
      301 taataaattg tttcgggtcg gtaattgggt ttgttggcca ttctttcttt tccttactta
      361 ttatggctcg ttttattcaa ggggctggtg cagctgcatt tccagcactc gtaatggttg
      421 tagttgcgcg ctatattcca aaggaaaata ggggtaaagc atttggtctt attggatcga
      481 tagtagccat gggagaagga gtcggtccag cgattggtgg aatgatagcc cattatattc
      541 attggtccta tcttctactc attcctatga taacaattat cactgttccg tttcttatga
      601 aattattaaa gaaagaagta aggataaaag gtcattttga tatcaaagga attatactaa
      661 tgtctgtagg cattgtattt tttatgttgt ttacaacatc atatagcatt tcttttctta
      721 tcgttagcgt gctgtcattc ctgatatttg taaaacatat caggaaagta acagatcctt
      781 ttgttgatcc cggattaggg aaaaatatac cttttatgat tggagttctt tgtgggggaa
      841 ttatatttgg aacagtagca gggtttgtct ctatggttcc ttatatgatg aaagatgttc
      901 accagctaag tactgccgaa atcggaagtg taattatttt ccctggaaca atgagtgtca
      961 ttattttcgg ctacattggt gggatacttg ttgatagaag aggtccttta tacgtgttaa
     1021 acatcggagt tacatttctt tctgttagct ttttaactgc ttcctttctt ttagaaacaa
     1081 catcatggtt catgacaatt ataatcgtat ttgttttagg tgggctttcg ttcaccaaaa
     1141 cagttatatc aacaattgtt tcaagtagct tgaaacagca ggaagctggt gctggaatga
     1201 gtttgcttaa ctttaccagc tttttatcag agggaacagg tattgcaatt gtaggtggtt
     1261 tattatccat acccttactt gatcaaaggt tgttacctat ggaagttgat cagtcaactt
     1321 atctgtatag taatttgtta ttactttttt caggaatcat tgtcattagt tggctggtta
     1381 ccttgaatgt atataaacat tctcaaaggg atttctaaat cgttaaggga tcaactttgg
     1441 gagagagttc aaaattgatc ctttttttat aacaggaatt caaatctttt tgttccatta
     1501 aagggcgcga ttgctgaata aaagatacga gagacctctc ttgtatcttt tttattttga
     1561 gtggttttgt ccgttacact agaaaaccga aagacaataa aaattttatt cttgctgagt
     1621 ctggctttcg gtaagctaga caaaacggac aaaataaaaa ttggcaaggg tttaaaggtg
     1681 gagatttttt gagtgatctt ctcaaaaaat actacctgtc ccttgctgat ttttaaacga
     1741 gcacgagagc aaaacccccc tttgctgagg tggcagaggg caggtttttt tgtttctttt
     1801 ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt ttggtcggca ctgccgacag
     1861 cctcgcagag cacacacttt atgaatataa agtatagtgt gttatacttt acttggaagt
     1921 ggttgccgga aagagcgaaa atgcctcaca tttgtgccac ctaaaaagga gcgatttaca
     1981 tatgagttat gcagtttgta gaatgcaaaa agtgaaatca gctggactaa aaggcatgca
     2041 atttcataat caaagagagc gaaaaagtag aacgaatgat gatattgacc atgagcgaac
     2101 acgtgaaaat tatgatttga aaaatgataa aaatattgat tacaacgaac gtgtcaaaga
     2161 aattattgaa tcacaaaaaa caggtacaag aaaaacgagg aaagatgctg ttcttgtaaa
     2221 tgagttgcta gtaacatctg accgagattt ttttgagcaa ctggatccag gagaacaaaa
     2281 acgatttttt gaggaaagtt ataaattatt ttccgaacga tatggcaagc aaaatattgc
     2341 ttatgcaaca gttcataatg atgagcaaac ccctcacatg catttaggtg ttgtgcctat
     2401 gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt caagaactgt tatggctaca
     2461 agataaattc cccgagcata tgaaaaaaca gggttttgag ttgaagcgtg gtgaacgtgg
     2521 ctctgaccgt aaacatattg agacagctaa atttaaaaaa caaactttgg aaaaagagat
     2581 tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa tggactgctt atagcgataa
     2641 agttaaatca gatttagaag taccagcgaa acgacacatg aaaagtgttg aagtgccaac
     2701 gggtgaaaag tccatgtttg gtttgggaaa agaaataatg aaaacagaaa agaaaccaac
     2761 caaaaatgtt gttatatcgg agcgtgatta taaaaactta gtgactgctg cgagagataa
     2821 cgataggtta aaacagcatg ttagaaatct catgagtact gatatggcga gagaatataa
     2881 aaaattaagt aaagaacatg ggcaagttaa agaaaaatat agtggtcttg tagagcgatt
     2941 taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa aacaagtctt taaagtctaa
     3001 aataagcgat ttaaagcgtg atgtgagttt aatctatgaa agcactaagg aattccttaa
     3061 ggaacgtaca gacggcttaa aagcctttaa aaacgttttt aaggggtttg tagacaaggt
     3121 aaaggataaa acagcacaat tccaagaaaa acacgattta gaacctaaaa agaacgaatt
     3181 tgaactaact cataaccgag aggtaaaaaa agaacgaagt cgagatcagg gaatgagttt
     3241 ataaaataaa aaaagcacct gaaaaggtgt ctttttttga tggttttgaa cttgttcttt
     3301 cttatcttga tatatataga aataacgtca tttttatttt agttgctgaa aggtgcgttg
     3361 aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct taaaattggt tgcacagaaa
     3421 aaccccatct gttaaagtta taagtgacca aacaaataac taaatagatg ggggtttctt
     3481 ttaatattat gtgtcctaat agtagcattt attcagatga aaaatcaagg gttttagtgg
     3541 acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa gaaaatcgct aatgttgatt
     3601 actttgaact tctgcatatt cttgaattta aaaaggctga aagagtaaaa gattgtgctg
     3661 aaatattaga gtataaacaa aatcgtgaaa caggcgaaag aaagttgtat cgagtgtggt
     3721 tttgtaaatc caggctttgt ccaatgtgca actggaggag agcaatgaaa catggcattc
     3781 agtcacaaaa ggttgttgct gaagttatta aacaaaagcc aacagttcgt tggttgtttc
     3841 tcacattaac agttaaaaat gtttatgatg gcgaagaatt aaataagagt ttgtcagata
     3901 tggctcaaag atttcgccga atgatgcaat ataaaaaaat taataaaaat cttgttggtt
     3961 ttatgcgtgc aacggaagtg acaataaata ataaagataa ttcttataat cagcacatgc
     4021 atgtattggt atgtgtggaa ccaacttatt ttaagaatac agaaaactac gtgaatcaaa
     4081 aacaatggat tcaattttgg aaaaaggcaa tgaaattaga ctatgatcca aatgtaaaag
     4141 ttcaaatgat tcgaccgaaa aataaatata aatcggatat acaatcggca attgacgaaa
     4201 ctgcaaaata tcctgtaaag gatacggatt ttatgaccga tgatgaagaa aagaatttga
     4261 aacgtttgtc tgatttggag gaaggtttac accgtaaaag gttaatctcc tatggtggtt
     4321 tgttaaaaga aatacataaa aa
//
LOCUS       DAGCWU010000175         4285 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.180, whole genome shotgun sequence.
ACCESSION   DAGCWU010000175 DAGCWU010000000
VERSION     DAGCWU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.180"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..868
                     /locus_tag="MBZ71_002483"
     CDS             <1..868
                     /locus_tag="MBZ71_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBT4430383.1"
                     /translation="SARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVPQHVHADE
                     QGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLASAPNYNLF
                     GVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTSFSTGDYH
                     YAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTGSATTTTS
                     TTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMAKNGMSMS
                     SYSLLVGQELNV"
     gene            1008..1934
                     /gene="cysK"
                     /locus_tag="MBZ71_002484"
     CDS             1008..1934
                     /gene="cysK"
                     /locus_tag="MBZ71_002484"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBT4430384.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2170..2616
                     /locus_tag="MBZ71_002485"
     CDS             2170..2616
                     /locus_tag="MBZ71_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBT4430385.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2889..4241
                     /locus_tag="MBZ71_002486"
     CDS             2889..4241
                     /locus_tag="MBZ71_002486"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBT4430386.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT         1461 a          721 c          912 g         1191 t
ORIGIN      
        1 cagtgcccgg caccatagac ctaaaagaaa aatggtgatc aataaaaaag cgattgctcc
       61 tttaattgga acgagtcttt tagttgggcc gatgatggtt acagcggtac cgcaacatgt
      121 tcatgcagat gaacaaggat ctcaaacagt cgctgatcct caagcgttta tcgatcaaat
      181 cggctggtct gcgtcagaag ttgctgcaag taatgacttg tatgcttcag tcatgatcgc
      241 tcaagcgttg ttggaaagtg catatggcac ttccggatta gctagtgcgc caaattataa
      301 tctattcggt gtgaaaggaa gctataacgg acaggtcgtt tatatggcaa ccaaagaata
      361 tgtaaacgga caatgggtaa cgatgaatga gccattccga aaatattctt cttattggga
      421 atccttccaa gaccatgcga atgttcttcg ttcaaccagt ttttccacgg gcgattatca
      481 ttatgctggc gtgtggaaaa gcaatacaac aagtttttat gatgcaacag cgtacttgac
      541 aggtcgttat gctaccgatc caaattatgg ggccaaattg aactcattga ttaatactta
      601 taatttaacc cgttttgata cgccatcaac aggttcggct acaacaacca caagtacaac
      661 aacaacgact actacgacga cttcaacgtc atcatcttca acaacagatg taaatacaaa
      721 taatggtcag acatatacag tcgtgtcagg tgatacgcta tgggatattg cacaaaaatt
      781 tggcatgtct gttgatgatc taatggcgaa aaatggcatg tctatgtcca gttattcttt
      841 attagttggt caagaactaa acgtttgatt tagataaaaa ggcaaagaaa ttaccatata
      901 tggtatatct ttgtcttttt tttgctagtc aaagacgaat cactcgccat aaatgaacag
      961 attgtattat gataagtaag aagagcaaag acaaggagga attgaagatg acagctatct
     1021 ataactcagt caccgaattg attggcaaaa cgccaatcgt caaattaaat aaaatcgtac
     1081 cagaagattc agcagatgtt tttgtaaagc ttgagttttt taatccagga ggaagtgtaa
     1141 aagatcgtat tgctttaagt atgatagaaa aagcagaaca cgatggtcta ctgaaaccag
     1201 gtgatactat cattgaacct acttctggaa atacaggaat cggattgtcg atggtaggag
     1261 ttgcaaaagg atataaagta atcatcgtga tgcctgagac gatgagtatc gaaagacgtt
     1321 tattgatgaa agggtacggc gcagagttga ttttgactcc tggtgcggat ggtatttctg
     1381 gatcaatcag agaagcagaa cgtttggcaa aagaaaacgg ctacttttta ccattacaat
     1441 ttgaaaatga agcaaatcct cttgtgcatg agaagacgac tggtcctgag atacatcaag
     1501 catttggagt aaatggcttg gacgcgtttg ttgcaggtat cggtacagga ggaacgatca
     1561 ctggcgctgg ccgagaatta aaacgtgtgt atccgaaaat cgaattaatc ggggtagaac
     1621 cagcagaatc tgctatttta gaagggaaag aagcgggacc tcacaaaatc caaggaatcg
     1681 gtacaggttt tgttcctaaa acactggata cttctgtgta tgataaagtc ctttctatta
     1741 gtggagacga agcgatggaa acagctagag aagttgggcg aaaagaaggg attttagtag
     1801 gcatctcatc tggcgctgca attgctgccg cgctaaaagt cgcaaaagaa ttaggaaaag
     1861 gcaagaaggt attagctgtg gttcccgaca atggtgaacg ttatttatct acagcccttt
     1921 accaagagat ataaggcact tggtttttct ccagataggt ttatgtaaaa aacttttaaa
     1981 gatgaaaaga acacacaaaa atttatgatg agaaaaatta tttgaataga gacgtagaat
     2041 agtgaacgcg actgttttac gtcttttttc attaaaatac aataaaattg aattttcttt
     2101 tcatgttaag tggattttaa aatgtaaaaa tagtatgata agacgagaat aacaacgtgg
     2161 ggtgtttaca tggaaaataa gttagtcgaa gaggttatcg aaaagttaaa aaaagagaac
     2221 attcgaatca cacctcaacg gtacgcggtt ttagaatatt tgattgaaca tcattcccat
     2281 ccaacggctg atgagattta taaagcactt gaacatcggt ttccaaatat gagtgttgcg
     2341 actgtttaca ataaccttcg cttatttact tcgattggat ttgttcaaga aatgaagtac
     2401 ggcgatgctt ctagccgatt tgatttcagt tcaaagcgtc attatcatgt aatttgtgaa
     2461 aactgcggta agattgttga ttttcattac ccagggcttg aagatataga aatggctgca
     2521 agcagactga ctgattttga gattaatgaa catcggttgg aattgtatgg actctgtccg
     2581 gattgtaaag agtcagcaaa aaaggcgcaa atgtaaaaac agtttctaca agtgttatac
     2641 ctaaaaaaag ctagctgtgt aaaaaaaacg ctagcttttt tatttttttg tcttttattc
     2701 acaatcttag atataaaagt gaatattatt aaaataagaa tcttgtagat attgggttct
     2761 tatgcttgaa aaggaatgaa actacaaaca aacctttgtg aattcacaag cattaaactg
     2821 ttgtatccgt tttatttctg ttatagtagt tttgtcgaag gacaaaatgt taatcggagg
     2881 gaccataaat gaaagtagta gtaattggct gtactcatgc aggtactgca gctgtaaaaa
     2941 gtattttaaa aaatcatcca gaagcagaag tgacagtata tgaaagaaat gacaatattt
     3001 ctttcttatc ttgcggtatc gcactttatg tcggcggagt agtaaaggac ccagcaggct
     3061 tgttctactc aaatcctgaa gaattatctt cattaggagc aacagttaaa atggaacacg
     3121 atgtaacaga cgtcgataca gaaaataaaa cagtaacggc aaaagatctg aaaacaggag
     3181 aaacagaaac ggtttcttat gataaattag tgatgacaac tggttcatgg cctattatcc
     3241 caccgattag tggaatcgat gcgaaaaatg tactcctatg caaaaactac aatcaagcaa
     3301 atgaaattat tgcacaagcg aaagaagcaa aacgtgtagt aattgttggt ggtggatata
     3361 tcggtattga attagtagaa gcttttgtcg aatctggaaa acaagtgaca ttgattgatg
     3421 gattagatcg catcttgaat aaatatctag ataaaccatt cacagatatc ttagaaaaag
     3481 aattgacaga ccgcggtgtt actcttgcgc taggcgaaaa tgttactgaa tttatcacag
     3541 atgaagaagg aaaagtgaaa caagtagcta caccaacaga tacatttgat acagatatgg
     3601 tcattttatg tgtgggcttc cgtccaaata caaaactgat cgaaggaaaa gtagatacac
     3661 tgccaaacgg ggcaatcaaa gtgaatgaat acatgcagac aagtaatcca gacatttttg
     3721 cagctggaga ctctgcagta gtcaactaca atccaagtgg cacgcaaaac tatattcctt
     3781 tggcaacaaa tgccgttcgt caaggtttgc ttgtgggaaa taacttgaca gatcataaaa
     3841 tggcgtatcg tggtactcaa ggaacttcag gactttacct atttggctgg acgatcggtt
     3901 cgacaggtgt gaccaaagaa agcgcaactt tgaacgatct agaagtccaa gcgactgtat
     3961 ttgaagataa ttatcgtcca gagttcatgc caactactga aaaagtaatg atggagctag
     4021 tctatgaaaa aggaacgaat cgtatcgtag gtgctcagtt catgtccaaa tacgacatta
     4081 cacaatcagc aaataccatg tctttagctg ttcaaaataa aatgactgta gaagatctcg
     4141 cactttcaga tttcttcttc caaccacatt ttgaccgccc atggaattac ttgaacctac
     4201 ttgcacaagc agcgctaggc gaaatcgaaa ctgcacaata aagaaaaagg gttgtgacac
     4261 aattttgtca cgaccctctg tcttt
//
LOCUS       DAGCWU010000176         4282 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.181, whole genome shotgun sequence.
ACCESSION   DAGCWU010000176 DAGCWU010000000
VERSION     DAGCWU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.181"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            212..1513
                     /locus_tag="MBZ71_002487"
     CDS             212..1513
                     /locus_tag="MBZ71_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4430387.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1540..1800
                     /locus_tag="MBZ71_002488"
     CDS             1540..1800
                     /locus_tag="MBZ71_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBT4430388.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2799..4034)
                     /locus_tag="MBZ71_002489"
     CDS             complement(2799..4034)
                     /locus_tag="MBZ71_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBT4430389.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1234 a          680 c          877 g         1491 t
ORIGIN      
        1 tagatttaat cattttgatt tttgttaatt atcgaatttt tttgttgctc ctatgattta
       61 gaaaacttat tttcaggatt cttaaaaata aatttgatta tttaagagaa tatattgaaa
      121 taaaatttct taattatata attagttatg aacattcttt gcgttgtcat gcaatgaatg
      181 gttcatattt tattgaaaag gagtggtttt agtggaaaag ctgatgaaat gggtcgaaga
      241 gaaacttgtc ccaccaatgg caaaaattgg tacacagcgt catttattag caattcgtaa
      301 tggggttgtt tcaacattgt cattgatttt gattgggaca ttttttatgg tctttatcaa
      361 tttaccgttt cctggatgga atgaatttat tgccccttat tcggcaacga ttgttttacc
      421 ttttaggatg acgatgggat tgatggctat ttatgcaact tttgttatgg gttcggatct
      481 agctaaatca tatggattag atagtgtgac aggtggtata ttatctttag gaacgttctt
      541 tatgttacaa gttcctgtaa acgttttaac tcctgaaaag gcacctcttg gctgggtctt
      601 acctatgagt tctttgggag catctgggat gtttgcgggg atcttatcta tgatttttgc
      661 agtggaagta tatcgtttct ttaaaaatcg aaatattact atcaaaatgc cagaacaagt
      721 gccacctgct gttgcccgta gttttgaggc attgattcca ggtgcagtca ttttaacaat
      781 tacttggctg attcgaagcg tcgcgggctt tgatgtaaac gctaccttat tatcgttatt
      841 cgagccttta accaatattt tagggaataa tttgcttggt gttttactac caatgttttt
      901 gatccatttg ttgtggtcat ttggtatcca tggcatgagt attgtagggg ctgttgttcg
      961 tccaatgtgg ctgatcatgt tggatgagaa tgcaaaggca ttggcagatg gtacagctgc
     1021 cacccaatta ccctatatcg ctcctgaaca gttttatcaa tggacggtaa caattggtgg
     1081 tgcaggagca acgatcgtgg tatctgtttt gtttatattc ttctgtaaat caaaattctt
     1141 gaaagaggtt ggtcgttttt ctattattcc tggtatcttc aatatcaatg agcccatgat
     1201 ttttggagct cctatgatgt tgaatcctta catgttcatc ccgttcaact tagttccttt
     1261 agttttgact attatcagtt attttgcagt caaattagaa atggtcaata gatttactgt
     1321 tttgccaact tggacattac cagctccaat cggcggttat ctttcagcag gaaatgactg
     1381 gcgtgttgtt atcttgatca tgatcaacac attgatagca tttattatct attatccatt
     1441 tgtaaaagct tatgataaaa agatgctgtc agatgaacaa agtcgtggag aaaacgaagt
     1501 ccaaactgtc taagtttggt ttttagaagg tgggaaacaa tgtattggct gattttattt
     1561 tttgttttta tctttttatt aacggctagt caccttatat tgaatatgct agctacatat
     1621 catattcaaa tcaatcgatg gatttgggca ctggcatcat ttttgatcgt gattcttcca
     1681 aagatcattg taccgcatat gaatgttttg ttttcatggg ggacatatgt actttgtgga
     1741 atttttgcta tcaattttat gatcgagcag catagatggt ttgtcacatc gaagctttaa
     1801 atgttctaaa agtggttgtg acagaagtgg ttagcttcaa gaaataagaa gaaatttacg
     1861 aaaattgctt cttaaatttt tgtgaaatgt tggcttattt ttcgtttggt tgaacttatt
     1921 accgcagatg caagcttttg accaccgttt attcggaata tgggactgtg acaaggcttt
     1981 tgtcacagcc cttttttaca taaagattat tattgtttta actgttttgg cagaaagaag
     2041 gaggcaaaaa tgaaaatttt atcagcaatg gattaagaat tagtcgatct actaccaaag
     2101 tgatcaaggc aaatatagat ttgggagctt acaaccatat agtaggggct gcagtatggt
     2161 gtatgaatgg taatatgtat gatgagagtg tttattcaaa tcatgaaacg tgtatagaaa
     2221 taattgcgct gggaatagca atcagtcatg gtgaacgtga cttttagttt atcgttgctg
     2281 acgacataga acattctgac atgatctact cagcatgtgg ggattgctgg cagtttatat
     2341 aggagtatgc atcggattgc gagatcattg tctcaacaga agagggaggt tgattatcaa
     2401 agataaagtg gtaaatccta catttgattt agtaattgtt gaagggacta gcagcacaaa
     2461 tctctacaaa gcgatgcttc tagatctata tgacacattt taagaaaatg tatttgccta
     2521 attattatgg tctttctttt gaagaaacgt tgagatgatc caaccagaga aaggtgtcaa
     2581 agaatttgac taagaataaa tggcagacgc tacctatgct catttggatt gcgtcagatc
     2641 ttttatttta aggatgattt tgtgtttttt agacaccgta atttaagtaa ctctaacttt
     2701 tcaatccact atttactttt tttgtatttt cactgtctgg gtctcgaata gcgaaattac
     2761 tgatgagtct ctattaaaaa agctttttct acttgaaact atattgggtg gttgctcagg
     2821 aaggtttcga tttcttgaat tgcttgttgt ggaccattta ttggaaaaac ggcttgcagc
     2881 gtgcgtgcct gctgacgaat cttgggtgaa gaaacttcgc gcagagcaat ctgcagtttt
     2941 ctttcagtca ctggttttgt gagggcgatt gcgtttccgt attttacaca atagtcaata
     3001 ttacagcttt gctcaaattg catccccaca ccgataaacg gtttgccgga ttcacaggca
     3061 gtttggacgg tcccttcccc gccgtggata aaagaaaaat caatgaattc atgcaaaaga
     3121 tgactagaca gatattctgt tattaaaaga tcttctgagt cagattgaat gtcatcaagc
     3181 agatagtcaa taggtgcaat aaccgctaga tccggttgtt tttgtaagaa gaacaaaatt
     3241 ttgcgtaaca tacgcggatt gcctgaacta cccattgcga aatagataat acgttttctt
     3301 tcttgttgct ttcgctgtat aaaagttagc acttcttcag gcaaggaagt actgagctta
     3361 gcaaaaattg gaccaacgat tcgataattt tccggcagct gttcttcttc caaaaatagt
     3421 ttaggcgttg tgatgagatt tagatcagca tccatcattt gtaaacttgt ttttggtaaa
     3481 ggaagcattt ctttttgtgc agcgtgtttg aaggcattgg ggatccatcg ggtatttact
     3541 atacgctttt ttgtccagtg ccaaagggga ttgcttaaag ggccgaagaa acgtaactcg
     3601 tggggcatca gcgcatgctc tgattgaaaa aaaggttcac tcaaagcata gggtttaaca
     3661 tatactaaag gaatcttttt attgcgggcc gataaaaaaa ttgtcacatt cgaacctgtc
     3721 acaatcagtc gaggctcaaa cttagcaatc agactctgtt cactagtgat acgagaggca
     3781 accatttctt tggtaaaagg gtttttaagt gtacgcaact ggtcaaattt tatgatttgt
     3841 tcttcttgtt ttttcgtaag gatcggcgct agtaaatgat actcaaagcc actttctgtg
     3901 atcaaaggag caaaagtttt agaaaagcca aagaatacac agtaataacg gtctttcata
     3961 gctttggcaa cctcaatcat tcgtgttgtc tcagctaaat tgaatactgc aggagcaaaa
     4021 agaattgtct tcatgaaatc ctccttattg gaatgagtct atcccttcgt ttcttactag
     4081 tatactaaaa aacacagcgt tcgagtagcc tatgtatatt ttgaatatta aactgtctct
     4141 cgctgttttc acaacttcta aatttaaatt ttgtaatcag gttgaaactc caattttcaa
     4201 aaaaacacgc aacaattttt tgatcgattt atatgttgtg ccagataaat acgagaagtt
     4261 gtgtcattaa aaataaacat ga
//
LOCUS       DAGCWU010000177         4129 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.182, whole genome shotgun sequence.
ACCESSION   DAGCWU010000177 DAGCWU010000000
VERSION     DAGCWU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.182"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            133..2073
                     /locus_tag="MBZ71_002490"
     CDS             133..2073
                     /locus_tag="MBZ71_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBT4430390.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2160..4082
                     /locus_tag="MBZ71_002491"
     CDS             2160..4082
                     /locus_tag="MBZ71_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBT4430391.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
BASE COUNT         1394 a          703 c          853 g         1179 t
ORIGIN      
        1 aataaatcgt atatcaattt ttttagcgaa atttctattt attccaagaa tttcagttat
       61 tacggaatca ttttgtgctt aataaaaaga aaccgttttt atatactata aatgtagaaa
      121 ggcggtagga aaatgaaaaa aattattgca atcacatcct gtccagttgg tattgcccat
      181 acatacatgg cagcagaaaa cttagagaaa gcggggaaag cagttggcgc cgaggtaaaa
      241 gtcgaaacac atggttccat cggtattgaa aatgaattga ctgcaagaga tatcgaagaa
      301 gcagctggtg taatcatcgc agcagatact aaaatagata aatcacgatt cggcggtaaa
      361 cctttgataa ccgtaggtgt gcaagaagga atccaccatg cagatgagtt ggtgagagat
      421 attttagcag gaaaagcgcc agtctataaa agtacggagg caattatttc ttccgaaaat
      481 aaaagtgaat cagaaaattt aggtaaaaaa atctataaga gtttgatgaa tggcgtttcg
      541 tacatggtac ctttcgtagt tacaggaggg ttattgatcg cgatttcttt aacattagga
      601 ggaacagcta cacctgaagg gatcaagata ccagaaggaa cgatttgggc aacgatgaat
      661 agtatcggaa gtatcgctat ggggttgatg gttccaattc tatctgcatt tattgcgcaa
      721 agtattgcag atcgtccagg acttgttcct ggatttgttg gtgggatgtt agcggcaaac
      781 ggagcattat atggtagtga tgcgaatgcg ggattcttag gcgggatcat cactggattt
      841 ttagcaggat tcattgcttt aggaatcaag aaagtgcgag taccgaaagc tttacaatcg
      901 atcatgccaa ttattgttat cccaatttta ggatcattgg cagttggttt tgtgtttatc
      961 tatgttattg gagaaccagt agctcttcta tttagttcat tgactcactt tttagcaggg
     1021 atgcaaggag gaagtgaaat tgttctagct atgatcttag gagcgatgat tgcttttgat
     1081 atgggggggc cgttcaataa agtagccttt ttgttcggat ctggcttgat tgcagaaggc
     1141 aattattcca ttatggggcc aattgctgta gctatctgta tccctccatt agcattgggg
     1201 ctggcatcat tgattttaaa aaccaaattt acgaaaacag aacgagaagc tggtaaggcg
     1261 tccttagcaa tgggactgtt tgggattaca gaaggcgcta ttccgtttgc ttcaacagat
     1321 ccactacggg tgattccaag cattgtaatc ggggcaatga caggaagtgt gatcgcgatg
     1381 ctttcaggag tgacagatca tgtagctcat ggcggtccga tcgtagcggt tttaggagca
     1441 gtggatcacg tagccatgtt ttttgtagca gtgattgctg gtattgctgt aaccgtgcta
     1501 atgttaagaa tccttaaacc gacattacaa caaactgcgg ttgctgcagc aaatggtgtg
     1561 acagtagcag atctatcaga gactgaaaca agtgatgttt atacgaaaac tgaaactttt
     1621 gattctatca cggatattat gtcagatgaa gcaatcgttt taggtgaaac agcaacaaca
     1681 aaagacggag taatcgaaga tttagtccaa cgtttagaaa aaacagcggc gatcacagat
     1741 aaaaccagat ttaaagaagt catctatgat agagaacatg aaagtacaac gggaatcggt
     1801 atggggattg ctattccaca tggaaaatca gcttatgttc ttaaaccaac agtggcattt
     1861 gccagaagtc aatctggggt agattggcat tctttagatg gaaaacctgc acatatgatc
     1921 tttatgattg ctgtacctga aaacagccaa ggtgatatgc acttgaaaat actgcaacgt
     1981 ctatcacgta aattgatgga tgatgacttc cgtcaagcac tgatggaagc gccagataaa
     2041 acaacagttc accaattatt aagcgaaatg taacataaga tgaaaaatcg accgtgatga
     2101 gacataatag atgtgttatc atgatcgatt ttttatttag aagggagttt ctgcacatta
     2161 tgaatcagcg tcagcaatct ttgatctatc aattaagtga gaacaataat tatataacaa
     2221 caaaaaagct agcagagcat ttccatgtgt cagaaagaac gattcatagc gatttgaata
     2281 ccattgaaac ttggttgaga gacaaacatc tgcctttaat gtttgaaaga aaaaaaggta
     2341 caggtatctt gttgaatggt ctgcctcacg aaagggaaca actgaaacga gcgcttaatg
     2401 aagagactca aacagaaatg gataaaaaac agcttcgtca gttattgatt ttccatctgc
     2461 tagtagcaaa agacggattt agtcttgaag aactttcaga aaaactatat gttggaaaac
     2521 gtacgattcg taaagaattg acagagttgc aaagcttttt tgagtatcac catttacagc
     2581 tagtatccaa aacaaaacta ggaacttttc ttaaaggcga tgaacaagaa aaaagacagt
     2641 tgcttgttaa gacattacga aatatgcaga aagaagatcc acaaagtccg tcgttgaaag
     2701 aattcttcgc taaagataca ttaaaagtta tccaacatac attacaagat gttttttatg
     2761 aaaaccattt agaacagccg aatggactgg ctagtgtgga gattcacatc tactttatgc
     2821 tcgagcgaat gaaacagtac cagaaagtga aattaagtaa agacgaaaac gaagtcgtgg
     2881 aacacaccca agcacaaaag ttaagtagtc aaatattagc aaaactagcc accatctatc
     2941 caattgaatt ttctcctgat gaaatcaatt atctggcttt gcgtatcgcc aatcttttta
     3001 caaattcaca agcagccaca cgttttcaaa aggaatctag cgcacttgct gatcatttga
     3061 tcgaacaagt ggaacagttt ctaggctatt cattaaaaga agatcagcta ctaaaacaaa
     3121 atttaaggtc tcatttatct tcaacttatt ttcgactcca ttatggtttg cagatttcta
     3181 atccattgac gaaaaatgtg tttagtacgt acacacaact tttcttagtt ttacagttaa
     3241 ttttagaaga ttattttgaa aaggaaaatt tttatgttcc acaagaggag acagcttatt
     3301 taacgatcca tttccaagca gcgatcgagc gacacaaaca tcagaaaagt agaagatatc
     3361 aaacggtttt agttagtgaa tattcaaaag ctatggctac ttttctcgaa gcacggctga
     3421 acagagaact tccagaatta acgattatgg atctagttga atataagcca gaattaaaga
     3481 gacaagattt tccctctgtt gattttatct tggctacgtt accatttaaa catataagca
     3541 ttccagtaat cgagatctca ccaatgatca cagaaactga tctagcctat ttgacaaaat
     3601 atatgctgga gcatgtgcct atcaagaaga aaaaaacatt tgatctagca agctttactc
     3661 atccatttct gatctttcct caacttgaac ggacagaccc agccgatatt ttaaacttta
     3721 tgggaaacgt cttagtggaa catcattatg tggagccgga attcatcgat tctgtcttgg
     3781 aaagagacag tcactccagt actagagtag caccatttgt aacaatccct cacggtaatc
     3841 cgctgtatgt gaagcattcg atgatttcta ttgctacaat gaaagaacca gtcctatggc
     3901 acggggaaca gatacgtatc gtactgctat tagcaataaa gaaaaatgat ttgaaacatg
     3961 cagagttcaa aaaaatattt tctgttattc actatctaga aaagcagcca gaacaaatgg
     4021 ctcagttgat gcaatccaat catccgctag aaatcttaac gttgttatct agttatgaat
     4081 aagtagcgat taaaaaactg cctttatatt tataataatt ctatctatt
//
LOCUS       DAGCWU010000178         4105 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.183, whole genome shotgun sequence.
ACCESSION   DAGCWU010000178 DAGCWU010000000
VERSION     DAGCWU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.183"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..365
                     /locus_tag="MBZ71_002492"
     CDS             <1..365
                     /locus_tag="MBZ71_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430392.1"
                     /translation="TTIPTFADSTNEPTTVVDNNQELTPLDRNGIAIGNPTNDLTVTE
                     NYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSY
                     KSAGNNWIATYSGTLTRK"
     gene            375..578
                     /locus_tag="MBZ71_002493"
     CDS             375..578
                     /locus_tag="MBZ71_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430393.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            789..1373
                     /locus_tag="MBZ71_002494"
     CDS             789..1373
                     /locus_tag="MBZ71_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4430394.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1744..3069
                     /locus_tag="MBZ71_002495"
     CDS             1744..3069
                     /locus_tag="MBZ71_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBT4430395.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            3062..3412
                     /locus_tag="MBZ71_002496"
     CDS             3062..3412
                     /locus_tag="MBZ71_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430396.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            3409..3588
                     /locus_tag="MBZ71_002497"
     CDS             3409..3588
                     /locus_tag="MBZ71_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430397.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            3724..4017
                     /locus_tag="MBZ71_002498"
     CDS             3724..4017
                     /locus_tag="MBZ71_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBT4430398.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
BASE COUNT         1464 a          611 c          795 g         1235 t
ORIGIN      
        1 gaactacaat tcctacattt gctgatagta caaatgaacc aactactgta gtagataata
       61 accaagaatt aactccttta gataggaacg gaatagccat tggtaaccca actaacgact
      121 taactgttac tgaaaattat ttaatagttt ctccattact tgctcgagca gcttctaaaa
      181 cgatacgagt gacaggaacg atgatttttg ataaagttgg ttggccttct agacctcctc
      241 aatcacggaa ttggaaagaa aaaagaaatg gttattggta ctatggaaaa ttgtatttac
      301 aaagttataa atcagctgga aacaattgga ttgcgactta ttcaggaacc ttaactagaa
      361 agtagggatt ttttatgcca gaatttgtga acacaccaaa tttttatcaa gatagcgata
      421 ttctaagtaa aattaattta ccgaaagaag aatcagcttt acaagttcag cgaactttac
      481 aggagacaca aaaaattcaa aaatcaattt ctaatctaga agattctgaa gaaaaaagaa
      541 aaaaaagaga ccaaaaaaca tatcaaaaag aaaaataatg attttaaaaa aagagtgtct
      601 agtcgacatt ctttttttct aattgaaagt gttcataaaa tcacccattt ctggacactt
      661 cctttttttg ctttattttt caaaaaaaca attcatttca tgttcataaa cccataacaa
      721 tatctatgtt cagttaaact atatggataa agttatgtta caataaaatt aatgaaggag
      781 tgattttttt gaaaatagga tacgcccggg tatctactgg tttacaaaat ttggatttac
      841 aagaagacag cctaaataaa tttggttgtg aaaaaatttt tactgatcat atgagtgggg
      901 caaaaagtaa tcgaccagga ttagaaatgg ccattgattt tgttcgctct ggcgatacac
      961 ttgtcgtttg gcgcttagat cgtttaggta gaaatatgga agatttgatt tcaatcgtca
     1021 atcgactgaa tgagcgtggt gtgagttttc atagtattca agaaaatatt acgatggaca
     1081 aatcaagttc tacaggacag ctcatgtttc atttgtttgc ggcatttgca gaatttgagc
     1141 gtaatttgat cctggaacga tccgcagcag gacgagcagc tgctcgtgca agaggacggt
     1201 ttggtggtcg tcctgaaaaa ttgtctgaac aagatttgga attactgaag actttagtgg
     1261 atagcggtac acctattaag actattgcgg aacggtggaa cgtctcaaga accaccattt
     1321 atcgttattt ggcaaaaatg gaggagaaaa atggaaagac aaattctcat tagtttttgt
     1381 catttaatgc tgaaaatttt tgccatttat ctagaaaagt gacaatagta tgagaatttt
     1441 ttgtaagtgt ctactgataa cttattatca aatgttgtaa ggattttatt catttgttaa
     1501 tattaaagaa cggaactcca aaaaagtccg tactctaatt tgcagtctct cactctttta
     1561 gagtgagagt ttttattaga ttttcaaatt aattagtaaa gtgatagaat aaattgatca
     1621 tacaaatcat aagaagaagc agggtgatag catacctatg cttctttttt tatgtattta
     1681 gctatttctt ctttacaaca tcgaacatat gtttgtataa tgtggaagaa tggagtgtta
     1741 tttatgatgg attattcaaa agagccagta aatgattact ttttaatcga tatgaaaagc
     1801 ttttacgcaa gtgtcgaatg tatagaaaga aatttagatc cattaacaac agaacttgtt
     1861 gttatgagtc gatctgataa tactggttca ggattgatat tagcttcttc tcctgaagca
     1921 aaaaagcggt atggtattac aaatgtgagt agaccacgtg acttaccaca accatttcct
     1981 aaaacactac acgttgttcc accacgtatg aacctatata tcaagcgaaa tatgcaggta
     2041 aataatattt tcagaagata tgtggctgat gaagatctac tgatttactc gatcgatgaa
     2101 tcgattttaa aagtgacaaa atcactgaat ctttttacga ctgaagaaac acgaagtcag
     2161 cgaaggaaga agctcgctca aatgatccaa gaacgtatta aagaagagtt aggtttgatt
     2221 gctacggtag gcgtcggcga taatcccctt ttggctaaat tagccttaga caatgaagca
     2281 aagcataatg aaggatttat tgcggaatgg acgtacgaaa acgttcctga gaaagtatgg
     2341 aatattcccg aaatgacgga cttttgggga attggttctc gaatgaaaaa aaggctcaac
     2401 caaatgggaa ttttgagcat tagagacctt gctaactgga atccttatac gattaagaat
     2461 agattaggtg tgattggatt acagctgtac ttccatgcta acggaatcga tcgaacagat
     2521 atagcaatcc cgccagaacc gacaaaggaa aaatcttatg gtaattcaca agtgttacca
     2581 cgagattata ctagaagaaa tgaaatcgag ctggtggtaa aagaaatggc ggaacaagta
     2641 gccattcgta tcaggcagca taattgtaaa acaggatgtg ttcatttaaa cattggtacg
     2701 tcgatcttag aaacgagacc aggattttca caccagatga aaatacctat cacagataat
     2761 acaaaggaat tgcagaatta ttgtcttttc ttgtttgata agtattatga aggacaagaa
     2821 gtaaggcacg ttggaataac ctatagcaag cttgtgtaca ccgattcgct tcagctggat
     2881 ttatttagtg atccacaaaa gcaaattaac gaagaaaatt tagataaaat tattgacaag
     2941 attcggcaaa aatatggttt tacgtcgatt gtacatgctt cttcaatgtt agaaagcgct
     3001 agaagcatta ctagaagcac acttgttggt ggtcatgcgg gcggaaatgg gggaattaaa
     3061 aatgattgat tacaatgatc gtggacgttt aaagtgggca gggttttatt tatctgatca
     3121 tactgaagaa attgatgccg acaatgaaca acgaaccagt caaaatttag caaaagaaca
     3181 aatgacgact gaagagattg cggaagtatt gaacgctgca gtcataaaaa atagatcagt
     3241 atctattcaa aaagaagaac gaaacgctga aggatactat ccagaagata ttgttggcaa
     3301 gatcttaggt agtgatgagc tggggatcta cgttggaaaa gacaaaattc actacgacga
     3361 aatacgtcat atcgaatttt accaggataa taaatggagt cgattagaat gaacattatc
     3421 cagcaatatg agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat
     3481 ggccaacgtt tgataaatga ggttggtgtt gagcgcttct ctttctatgt agaagctgca
     3541 gcaggttatc ataattttag gttttatatt ttcccgttat atatataagg tattttacgg
     3601 attctataga gttatctcta tgaatccttt tttgttttta caaataggag tagatactag
     3661 aataatgcgt gtgttaaact attattgcaa acgcaataat aaaagaaagt aggcttacgc
     3721 accatgtcta tcaatacatt tgtttactcg cacccgatca atgtttacat tatcaagaat
     3781 ttaggaatca ctgtagaaca attttgtgag ttatatgcct acccacaagg aacggttgca
     3841 agctggataa cccgacaacg tagaataaaa tcattacctg cttcatttgt atatgatcta
     3901 agtcttgctt ctagtatgaa tatgaccgat gtatacgaaa agttattaat actagaaaat
     3961 gaatatgatc aattcaattc aaatcagcag cgaaaagtaa agaaacagat agactagaat
     4021 agctattttc gaaaagcaat atttaaggta taagttatac cttaaataat aaaagtataa
     4081 gttataccat aaatatatat ggtat
//
LOCUS       DAGCWU010000179         4091 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.184, whole genome shotgun sequence.
ACCESSION   DAGCWU010000179 DAGCWU010000000
VERSION     DAGCWU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.184"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            34..873
                     /locus_tag="MBZ71_002499"
     CDS             34..873
                     /locus_tag="MBZ71_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4430399.1"
                     /translation="MPRKPLSEEDKKARSIIANNLKKITHGYTRAQLSDMTGIPVTTL
                     SGYFTEKSTISMENAELIAKAFNVDKSDIDPRYSREYNYYDLKKEVLDELQENNKSFV
                     ADKFVELAEKANAAGMTLNRTKTDRGNIMTVLSSDIQEYNFFDTSVAAGIPSSVEAFD
                     QDHIEQIAIPDYAMGKYAGYQDVFFTKINGDSMNNVIPDGSLIAVKKIESCDELKDGD
                     IVVFSNDNEFSVKRYINDQQNNRIIFRPDSSNISFTDIVVNYDDTKYVQIYGKVVVYI
                     VQL"
     gene            894..1094
                     /locus_tag="MBZ71_002500"
     CDS             894..1094
                     /locus_tag="MBZ71_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430400.1"
                     /translation="MKIGMRKPSIKKSIGARTTGKAKRAVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            1180..1620
                     /locus_tag="MBZ71_002501"
     CDS             1180..1620
                     /locus_tag="MBZ71_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430401.1"
                     /translation="MKFIKFGIIGLLVVTVMGGCGTSTAETKESKNYSETNSTIKEVD
                     SDQTAEFATIAEKHIKEIYSIDNLKMDLKSIKVTQFPDDKNAETGEEYKNVYNGTGDF
                     TWQDKKYNFSLIYSKKDATNYTVLYLYSNLDPNKGVDTPLESDQ"
     gene            1759..2964
                     /locus_tag="MBZ71_002502"
     CDS             1759..2964
                     /locus_tag="MBZ71_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4430402.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIEFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQSENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(3016..3103)
                     /locus_tag="MBZ71_002503"
     tRNA            complement(3016..3103)
                     /locus_tag="MBZ71_002503"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3069..3071),aa:Ser,seq:cga)
     gene            complement(3248..3814)
                     /locus_tag="MBZ71_002504"
     CDS             complement(3248..3814)
                     /locus_tag="MBZ71_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4430403.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         1522 a          651 c          687 g         1231 t
ORIGIN      
        1 gatattatta ataaaaaagg aggagaaaat aacatgccaa gaaaaccatt atcagaagaa
       61 gataaaaaag ctcgctccat tatcgctaac aatttaaaaa aaattacgca tggctacaca
      121 agagcacaat tgtcagatat gacaggtatt cctgttacga cgctatcggg atattttact
      181 gaaaaatcaa ctatcagtat ggaaaacgct gaattaatag ctaaagcttt taatgttgat
      241 aaatctgata tagatccaag atattccaga gaatacaatt attatgattt aaagaaagaa
      301 gttttagatg aattacagga aaacaataaa agtttcgttg ctgataaatt tgtagagtta
      361 gcagaaaaag caaatgcagc agggatgact ttgaacagaa ctaaaactga tagaggaaac
      421 attatgacag ttctatcaag tgatattcaa gaatataact tttttgacac ttctgttgct
      481 gccggcatac cttcttcagt cgaagctttt gatcaagacc atattgagca aattgctatt
      541 cctgattatg ccatgggaaa atatgcagga tatcaagatg tattttttac taaaataaat
      601 ggagattcca tgaataacgt tattcctgat ggatcattaa ttgctgtaaa gaaaatagag
      661 agctgtgatg agttaaaaga tggagatata gtagtcttta gcaacgataa cgaattttct
      721 gtaaaaagat atataaatga tcaacaaaat aatcgcatca tttttagacc tgattcttct
      781 aacatttctt tcactgatat tgtggttaat tatgatgata ctaaatacgt tcaaatttat
      841 gggaaagtcg ttgtatatat tgtccaacta taattaattt gagagggtat aaaatgaaaa
      901 ttggaatgag aaaaccgagc ataaagaaat ctatcggcgc tagaactact ggaaaggcaa
      961 aaagagctgt gaaaaaagct gtgattcctg gttatggaaa gaaaggctct ggatggatta
     1021 aagacccaaa aaaagctgca tacaataaag tttataaaaa gactacgttt agcttttggg
     1081 acttatttaa ataaaaaaag cccgtgcagc aacacggact cgaaacctta tttctaagat
     1141 tccccaataa aataatatca tagagataag gagtagaaaa tgaaatttat taaatttgga
     1201 attattggat tactagtagt gacagttatg ggaggatgtg gaacatcaac agctgaaaca
     1261 aaggaatcaa aaaattatag tgagacaaac agtactatca aagaagtaga ctctgatcaa
     1321 accgctgagt ttgcaactat tgccgagaaa cacattaaag aaatatattc aatagataat
     1381 ttgaagatgg atttaaaatc tatcaaagtt actcaatttc ctgacgataa aaatgcagaa
     1441 actggtgaag agtacaaaaa tgtctacaat ggtactggag attttacttg gcaagacaaa
     1501 aaatacaact tttctttgat ttattcgaaa aaagatgcaa ctaattatac cgtcctatat
     1561 ctatatagca atctagatcc taataaaggt gtagatacac cattagaaag cgatcaataa
     1621 aaaaagcaca tttaccgccc gaccaagaac gcaaatgtgc aaagagctac ggtaaagtag
     1681 gtctatttgt tgtaccctat tttaccatct cgaaatccgc tgtacaaccg aacaaacgta
     1741 cgaaaggaaa gataaaagat ggcaaaaaaa gaaattgata aacgaattaa aaagcactta
     1801 acaaaaaaag gagaagaaaa atatcagttt agtctgtact taggagttga tccgctaaca
     1861 ggaaaaaaaa gaaaaacaac gagacgaggt tttagtacgc cattagccgc tgagagagct
     1921 ttaaaaagat tggaggcaga tatacaggaa aaaggactgc aagcttctac agctcaaaag
     1981 agcaagaaat tcgaatatgt ttacggctta tggtttgaaa attataaaaa gacagttaaa
     2041 gaaagcactt ggtcctccac caaacaaatt tttgacacac acatcttaaa agtgtttgga
     2101 gataagttca ttgacaaaat agacgttttt ttctgtcaag aagcagtaaa cacttggtcc
     2161 gatagccatc ctaaaatttt taagaaaata aaaaattaca catctaatgt ttttgattac
     2221 gctgcttctt tacaaattat aactagcaat cctatgaaat tagtttctat tccacgtgga
     2281 gaagctctag atatcgaaga taaaaatatc gaattttata ctaaagaaga attaatcgaa
     2341 tttttagaag caataagaaa cgatgatgat gaaagatatt tattcttttc cctattggct
     2401 tttacaggta taagaaaagg agaagctttt gcactgacct ggtcggatat tgattttaag
     2461 agcaaaacac tcaatattaa taaaactgta acaagaggtt atcaagggag attgatcgta
     2521 aatactccta aatcaaaatc tggaaaaaga aaaatttatc tggacaatga tttgatcagt
     2581 ttattgagaa agtattatac aaaaaacaaa acgatcgtta caatccaaag cgaaaactta
     2641 atttttcacc atgatggtct tctctataat cctacagttt cacgttcgtg gcttaatgtc
     2701 atatataagc atcatccaga attaactaaa agaatcacta ctcatggttt tcgacatact
     2761 catgcctctc tcctttttga atcaggagct tctttaaaag atgttcagga acgtttaggg
     2821 catgcagaca ttcaaactac ttcaaatatt tacactcatg taactgaaac tcaaaacaaa
     2881 aaagtcatca ataattttgt agcatttatg aagaatagta ctcaggggga gtcaaaaggg
     2941 gagtcaaaaa acattttagg ctaaattaca acaaaaaaag acacgatcga aatcgtgtct
     3001 aatcgttgat atataaggaa ggtacaggat ttgaacctgc gcgccggtat tagccggttc
     3061 gccggatttc gagtccggtg cattaccact ctgccaacct tccgtaagta ctatttttaa
     3121 acttttttca cgaaatgtca agcctatttc taatagataa ataacagaaa tcgctttttt
     3181 tagcaaaaaa gtccattgac agtttgcagc caacggactt accgatcaaa ttcatactga
     3241 tagaacacta tctgattatt tcaggattat ctaatgctaa aatcgagtca attgtccgtt
     3301 tgatcagtgc aatgatttgt tcttctgttg gatgcatgat gtttgatgac atcaatgacg
     3361 ctaagccagt cactgctacc catgtaccag tatgcagagc atcgacatat tcatccgata
     3421 attgattgta tctttcatct tctttaacta tttcacggaa ataattgtat gaaaaatcat
     3481 gcattttctt tccgtcccca tgttcatcaa tgtacaatgc catgtaaaga ctatgatgct
     3541 tttttgcaaa gtagatatag ttcaaaccga gatcaatgat tttatcacca gtacgttcaa
     3601 tagggaaaac atcttctttt agttgttcaa agatagaatc cagcaacgta ttttttaaat
     3661 cttccatgtt tttgaattcc agataaatcg gctgtgtcga aatccccatc tttttagcaa
     3721 tatttctcgc agtaaatcct ttaaaccctt cctttgcaac aacatcataa gcagctttta
     3781 gaatttgatc cttggtatag acttttcttc tcattaaaac tcccccactt ctttactgca
     3841 gtatatgttg ttttattgta ccctattttt agcatttttt gcaaacattt tactcaaaac
     3901 gttatatttt aaattttttt tcagtcttct ttaacttttt tttgcaaaat taaaaaaatt
     3961 tggttaaatt caatgggttc ccttacattt ttagttttga cctaaatgat agttgtccaa
     4021 atctttcatt tcaagttata gttaaagaaa atgaaaaaga aggtgtgtaa tggaaaacga
     4081 aactgacgtt a
//
LOCUS       DAGCWU010000180         4005 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.185, whole genome shotgun sequence.
ACCESSION   DAGCWU010000180 DAGCWU010000000
VERSION     DAGCWU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.185"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            24..1373
                     /locus_tag="MBZ71_002505"
     CDS             24..1373
                     /locus_tag="MBZ71_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4430404.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1501..2091)
                     /locus_tag="MBZ71_002506"
     CDS             complement(1501..2091)
                     /locus_tag="MBZ71_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBT4430405.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2238..3590
                     /gene="brnQ"
                     /locus_tag="MBZ71_002507"
     CDS             2238..3590
                     /gene="brnQ"
                     /locus_tag="MBZ71_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBT4430406.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1151 a          738 c          779 g         1337 t
ORIGIN      
        1 tttttagtta ggatgattta aaaatgaatg aagagcaaga actgcagcgt ggactgaaaa
       61 atagacatgt ccaactgatt tctattggcg gagcgattgg aacggggttg tttttaggtt
      121 ccggaaagtc tatccagcta gctggccctt cgatattgct cgcctatctt attactggat
      181 gcatctgttt tttcattatg cgtgcgttag gcgaacttct gttatcaaac accaataacc
      241 attcttttct ggattttgtt gcagaatatc ttggcaaaaa agcggctttc atcactggct
      301 ggacgtattg gttctgctgg gtatcgattg caatggccga tttaacagcc atcggaatgt
      361 atgtacgcta ctgggcgcct ggtgttcctc agtggcttcc tgaactgatt gcattaggtc
      421 ttcttctttg cttgaatatg gtggctgtct cattgtttgg tgagttagag ttttggtttg
      481 cattgatcaa agttgtagcg atcattgcct ttatcctcgt aggagcttat atgatcctca
      541 cccactatac gacagatatt ggcacagcca gtatcacgaa tctatggtct catggcggat
      601 tctttccaac tggtgcaaaa ggatttatct tagctttcca aatggtaacc tttgcttttg
      661 ctgggataga attagttgga ttagtcgctg gagaaacaga aaatcctgaa aaagtgttgc
      721 cggaagctat caataatatt cctatcagga tcatcttatt ttacttagga tctctttttg
      781 tcattatggc aatctatcca tggaacagct tgaatccaga taacagtcca tttgtagaag
      841 tattttcgga aattgggatc acgattgctg cttccttgat caatttagtc gtattaagtg
      901 cagcagcttc tgcctgcaac agtgcaatat atagtacagg acgtatgctt cgttctttag
      961 cgcaagaagg cagtgcccct aaaaaattca aaaagttaac aacacatcat gtgccaggga
     1021 atgctttgac attttctact atcgtcattt tcatttctgt tatcttgaat tacgtgatgc
     1081 ctagtgaagt attcacacta gtaagcagta ttgccactac ttgtttcttg tttatctgga
     1141 gtatgcttgt ctacacgcac atgaaatatc gtaaaagtat cctcggcaaa aaagctcatt
     1201 catttaaaat gccgttgtac ccatttagta attatctggt atttctttac atggcctttg
     1261 tgtgtgtcgt cttgttccta ggaaaagata cgagaatcgc tttattacta acacctgtgt
     1321 ggttcttgct tctattacta atctatcata tgaaatatga acgtaagaag taagaaagta
     1381 gttgtgacag aagtggtcag cttcgagaaa taagaaggaa tttacgaaaa ttgcttctta
     1441 aatttttgtg aaatttcggc ttatttcttg gagataaaag cttctgtccc agcctctttt
     1501 ctatttcccc ggaaacaacc gctgataata ggcatctgct tttttatagg aaaaaatcca
     1561 aataccagat acaaaaaaac gtccgaataa cccgatcaac ggtacaaaaa agacaccggc
     1621 aatcaagcat aagttaatca tcatggagat aagacttcga cggtcctttg acaaaactct
     1681 cacgacatgg gaatctggtt tatttgcttt catgatttgc ctcatcaaga taccaaaaga
     1741 aaaactccac atcgtgtata ctaagacata agccatctca ggccaaaaag cttcagggtt
     1801 acgcccgacc caactagtaa cagaaggaca taaagtcagc caaaagagca aaaagatatt
     1861 cgaccagacc actcggtaat taattcgttc tgccatctga aatatacgat gatggttcac
     1921 ccagatcacc gcaacaaata taaagctgat ggtataagct aataatgtat ttcggatgac
     1981 ccataaagaa tgaaagctag atgtattagg gatcttgatc tctaatacaa taactctcaa
     2041 aacaatggct accactccat cagtaaatgc ttctaatctc gactttgtca cgatgattcc
     2101 tcctcagatg attcagcgct tttattttat cacttacgtt attaaataac catcgtttta
     2161 tatagggaat tttctgaaaa ttgttgcata tttttcgaaa ttttgttact tttaaaaaaa
     2221 ccgtaaagga gttatgtatg acaaaaaaat tatcatttaa agactatctt tttataggct
     2281 ccatgctttt cggtctattt tttggtgctg gaaatcttat ctttcccgtt catttgggtc
     2341 aagaagcagg cgctgctacc ttttgggcga atctaggttt cttggttacc ggtatcggtc
     2401 ttcccttctt aggggtgatt gccattggtg tatcgaaaag ctcaggtgta tatgaattag
     2461 caaaacggat aaatcaaaca tatgcgatgg tttttaccat cttgctttac ttagttatcg
     2521 gaccgttttt tgctttacct cgccttgcaa ccacatcttt cgagattggt ttagcaccat
     2581 ttatcgatcc tagcagacag acgctatatc tagcaatttt cagtattgta ttttttgtgc
     2641 tggcttggtg gttttcaaga aaaccgacaa agatattaga ttatgttgga aagttcctga
     2701 atcctgcatt tttggtttta ttaggtgctt tgcttctcct tgcttttttg aatccactag
     2761 gttctgtaaa tcaagcgcct attcagccaa attatcaaga gcatgctttt ttcacagggt
     2821 ttactcaagg atataacaca ttagatgcgc ttgctgcttt agcatttggg atcatcattg
     2881 tcacaacaat ccaaaacatg ggtgtaacaa aacctgctga aatcgctaaa gacacgatca
     2941 aatctggtgc gatcagcatt atattgatgg gaatcattta tactttgctc gcttatttag
     3001 gtgctatgag cttaggtagc tttgctctaa gtgaaaatgg cgggatcacg ctggctcaga
     3061 ttgctgatca ttatttaggt acttatggca gtatcttatt agcatttatc gttatattag
     3121 cttgcttgaa gaccggtatt ggtttgatta cagcttttgc agaaacattc acagatttgt
     3181 ttccgcaaag aaattacgca ttctttgttg cacttgctag tatccttcct tgtttagcgg
     3241 caaacgtagg cttgacgaat atcattcaat tttcgttgcc tgttttgatg ttcatctatc
     3301 cattagcaat gacactgatt ttactagtgt tagttggtcc tttgtttaag cagcgtacgg
     3361 ctgtttatcg aatgactact tattttacat tgattgcttc gatttttgat gggttgaacg
     3421 cttgcccaga aactatcaaa caaacaccta ttgttcaaaa tcttttatat gttgcggaaa
     3481 gctacttgcc gtttttcaaa ttggggatgg ggtggatcgt accagcagtt atcggttttg
     3541 ttatcggact gatttggagt ttcgctaaga aagaagaagt agcagattga taaaagaggt
     3601 tgtgaaagag acgttcagcc ttgagcaata aaaagtaatt ttgaaaaatg gcttctcaca
     3661 tttttgcata aatttgactt aataccgaga aactgacttt tgaacaccgt ttattgagag
     3721 gtcgtgaaaa agccgcttag tttcaagtat taagaaatat tttgtgaaaa tggcttctca
     3781 catttttgca taaatttgac ttaataccga gaaactggct tttgaacacc gtttattgag
     3841 cgtctataaa ctgacctgag tattagagaa acaaaacggc aaagtagctc catatacttt
     3901 gccgttttgt tttgaactaa taccgaagag aagctgatca aacaacattt cttttataat
     3961 atagatttta gacatatata tgatataaaa atagttttcg caaaa
//
LOCUS       DAGCWU010000181         3955 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.186, whole genome shotgun sequence.
ACCESSION   DAGCWU010000181 DAGCWU010000000
VERSION     DAGCWU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.186"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            2..460
                     /locus_tag="MBZ71_002508"
     CDS             2..460
                     /locus_tag="MBZ71_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430407.1"
                     /translation="MLHLFLSKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIFSVL
                     LSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLII
                     FITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(586..1263)
                     /gene="nth"
                     /locus_tag="MBZ71_002509"
     CDS             complement(586..1263)
                     /gene="nth"
                     /locus_tag="MBZ71_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBT4430408.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1270..1968)
                     /locus_tag="MBZ71_002510"
     CDS             complement(1270..1968)
                     /locus_tag="MBZ71_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBT4430409.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2236..3420
                     /locus_tag="MBZ71_002511"
     CDS             2236..3420
                     /locus_tag="MBZ71_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBT4430410.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3426..3869)
                     /locus_tag="MBZ71_002512"
     CDS             complement(3426..3869)
                     /locus_tag="MBZ71_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4430411.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1031 a          778 c          643 g         1503 t
ORIGIN      
        1 tctgctccat ctttttttat cgaaaacaat acctcgttat tccaagaata aaaaagctga
       61 aaaattttgt ttattattaa ataaatttac tttaattgct cctatccttg catttattat
      121 tttttctgta cttttaagca ctactcttaa aggaaaattt atggaaagaa gctctcatgc
      181 aatgattctc acttttttat ggttgttgtt caccagaatc tatatttttc tgatgagcct
      241 gaaaccgccc aaatccatta gtttatgttt agttataaat ggtatttttt tattatcact
      301 tatcattttt attacacctt tagatagata cgtcacttat ttatataatc ctttggaata
      361 ctggacttat tttataggta tattggaagg cattattttc tacatcgggt attttcccaa
      421 taaaaataac aacttttatt tttatcgcaa taaattgtaa taagttacca tcctagctac
      481 tgagtatgta catttacttt ttcatgcaaa aaagataaac gaaacagctt ttctccataa
      541 taacaagata attcatacat ttatttgcaa ttaaagaaac cacgtctaca ggcgtggttt
      601 cttttttagc gccttttctt ttagacgcat ccgatttttc ccatcttgac atattgatag
      661 caaaggacat acctcgcact tgggatttct tgccgtgcaa tgatatctac caaaaaaaat
      721 cagtgtatga tgtgtcttta cccataattc ttgtggtact ttccgcatca gtgtttcttc
      781 tacttccatg accgtagcat ctagtttgca gatcctcagg cgttttgaga cacgttccac
      841 gtgggtatct actgcgattg ctggtatccc gaatgcatcc cccaatacga catttgctgt
      901 ctttctgcct acaccaggaa gagacatcaa ttcttctctt gacgttggca cttgcccatc
      961 gaaacgttca atcagctgct gtgcacacgc tttgatgttt tttgctttat ttcgatacaa
     1021 accaatcgtt tttattttaa gaatgatttc atcgattgaa gcttctgcta acgcatcggg
     1081 tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc actgatacat ctgtcgcctg
     1141 ggcacttaag atcactgcaa tcaataactc aaatggatta ttgtgcttga gttctccatg
     1201 tgcttcggga aacattccat acatcgtttc tagagcttcc attgtttttt gtttgcttaa
     1261 catattccgc tactcctcca accaattttt cagtgagact ttcggcaatt cttcttgttg
     1321 agctgactgt tctacttctc tttgaagaag ctgctgtttt cttcgttttt ggtcttcttc
     1381 cacttgttgt tttgtacgta gattttttct ttcccacgat agcaaaatgc ggtcaacgta
     1441 attaaaacta taagcctgat ttaatacggc ttcccgcagt gctagcttca aaatttccgg
     1501 ctgataatgg tcttcttcta gccattgtcc gatccgctgg aactcgatcg aagaaagcgg
     1561 gcgtccaaat tcttgttcaa ataattgata cactgactgg atttcttttt cttgtgtttt
     1621 ttcttcgtat tttttttctt gtgtttttaa atactcacct aatagatcat agatggggta
     1681 aagattatat cgatctgctt ttttaccgtt gacaatcgtc gtttctatct tgataaatcc
     1741 atttgttacg agacgatcca gtatttgaaa aatctgatct tgtttgattc ccatatccat
     1801 agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc tgtgccatat gcaactgtag
     1861 aacaaataaa aattcttcag attgtagacc gatcctatga taatttttaa gtaaaagatt
     1921 agatatgact gtttgtccag cgcttaagta attttctaaa ttcaacatga tttctcctcc
     1981 gctcaatcac agtatacgta aaaaaaacat gggaagcaag aacaggtttc ttttctattt
     2041 acttcactga tcggtcagat ttacaatgtc gtctacttga tttttgtttg ttcatgtttt
     2101 tgatgcgtat cttcactttt cggaataaat aaattaagta gatttcgctg ttccttagaa
     2161 tgttgacaag gaaacaaaat tactatacac tatttttgtt tccaagttaa cattattgca
     2221 taaaggagtt tttacatgta cagtttcaca aataaacatt ctaaagtttt tggatacgtc
     2281 tctgtcttat tcactggatt aggatttact atcgtcagtc ctgtacttcc gtttttaacg
     2341 ctgccttatt ctcactccat acatcagcaa gctttctata tcactttgct catgtctgtt
     2401 tatgctctag ctgccttctt atctgccccc attctcggta gcttaagtga tcattttggg
     2461 cgtcatccga tccttattat cagtttatta ggatcaagta tcggatattt gctttttggt
     2521 ttaggaaatt cgatatggat gttgttcctt ggtcgtatca ttgaaggatt gacggcagga
     2581 gaaatctcca ccttatatgc ttactttgcc gacatcacgg aacctaatga aagaaccaaa
     2641 gtttttggat ggatgggtgc tttagtcggt ataggaacaa cgctgggtcc tattataggg
     2701 ggtctattag ctgagttggg gaattctgtt cctatattta ttggagcact ctttactttc
     2761 ttaaatgccg tttatggcta cacatttatg ctagaaagcc ttccaataaa aaaacgttca
     2821 gtagatttaa gtttctccca tgtaagacct tttcaccaat tacaacaatt attcaagatt
     2881 tcatccgtta ttcctttatt gacagcaggt tttgctgttt ggttggcagc tggctctctt
     2941 caatctatct tttctcaatt ttcgattgac gcctttcagt ggaaggctgg gttggttggt
     3001 ttaagcttct ctttgattgg aatcttggat actgtctcgc aattgttcat tatgcctcga
     3061 ttgctcaaaa aattttcaga acagcagatc acaagaatag ggatgttcag tgaaatcctc
     3121 gcctatctat tcatcacatt atctggaatt cttctactgc cgattttgtt tttatttggg
     3181 attatctgtt atggtttcgg tgattctatt tttactcctg tattcaatgg ccaactatcc
     3241 aattctgtct cagataacca gcaaggtctt gtaatgggcg gtacccaaag tatacaatct
     3301 ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt atgctgcagc accatttctt
     3361 ccgacggtat tcggattttt gcttatgatt tttgcctatt atcactacaa taaaaaatag
     3421 tagcttcatt tgctacttcg tataaattct ctgtatcgac cgataaaacg aaatactgtg
     3481 tcaaattctt cttttgacat attttcaaat acagtagcat ctcgctgtac ccatttttga
     3541 tggagttgct cgtgtctatt ccttattttt tgtccgattg gcgtcaattg aaaatagatt
     3601 tcttttttat tctcttcgct ttgataagtg tttatgatcc ctctagctat cagttttttt
     3661 gttagcttac tgatggctcc tctagtcatg taactagcat tcgctaattt tgtcacgtta
     3721 ggatcaggga ttttcccgat atgttcaatc acttctagtt catttagagt aaatcctgca
     3781 aattcttttt ccatttctgg tttattacga tgaataatct cattagctaa atccagatat
     3841 tcttctatta gcagttgtct tttatccatt tctacacctt ctttttcttt cattatattg
     3901 tttttttgtt tcctattcaa caaagataaa aatattttgt aggaaaaccc aatct
//
LOCUS       DAGCWU010000182         3917 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.187, whole genome shotgun sequence.
ACCESSION   DAGCWU010000182 DAGCWU010000000
VERSION     DAGCWU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.187"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..477
                     /locus_tag="MBZ71_002513"
     CDS             <1..477
                     /locus_tag="MBZ71_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBT4430412.1"
                     /translation="LELYKSYKDSKDIKESHNDDLLESLSTKNNKLDEELIETFIEEN
                     LLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKY
                     FVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            553..771
                     /locus_tag="MBZ71_002514"
     CDS             553..771
                     /locus_tag="MBZ71_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430413.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            758..3436
                     /locus_tag="MBZ71_002515"
     CDS             758..3436
                     /locus_tag="MBZ71_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430414.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSETVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3427..3624
                     /locus_tag="MBZ71_002516"
     CDS             3427..3624
                     /locus_tag="MBZ71_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430415.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3628..>3917
                     /gene="mobC"
                     /locus_tag="MBZ71_002517"
     CDS             3628..>3917
                     /gene="mobC"
                     /locus_tag="MBZ71_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBT4430416.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREES"
BASE COUNT         1497 a          532 c          784 g         1104 t
ORIGIN      
        1 ctagaattat ataagtctta taaagatagt aaagatatta aagaatcaca taatgatgat
       61 cttcttgaat cactttcaac taaaaataat aaattagatg aagaattgat cgaaacattt
      121 attgaagaaa atcttcttaa acaaatatgg ggagaaagta ttgtgaattg cttaaaactg
      181 gctagcaata gtgattatcg tcaattcgac aactggtaca aaaaattcaa atatgcgatt
      241 agatctgctg aaaaagaaca aaaaattcaa ctgaaaatta tctatgaaat ttgtaataac
      301 aagtattttg tagatcatgt tagagaacag ctatcaatga ctttacgtga cttgatacga
      361 agagcaaaaa cagatcatcg tatcaaacaa aaagacgatt atatctttgc ttctctaaaa
      421 aataaagcgt tagaactaat tgaattgcaa atcagcgaag gaatagataa gcaataagaa
      481 tagaattgag tcaaatgaaa tggaggggta tctccacttc atttgagaac tttgaatata
      541 ggaggggtta ttttgattaa aaatcgttta atagatcaag tttggtgtct agatcaaaat
      601 acagatagtc tcgaattaat cgagactatt tgcttcaaca ctatagttct cgatttaaga
      661 gctgaaaatg ataattgtgt aactcatgtc ataattaatc aaaaaatggc tcagaagctc
      721 tctgagagcc tcagaaaatg ggctaaggga ggtaacgatg aaaataattg aaaaaaagat
      781 tgccaataaa aaaataaatt ggttggttgt taattttctt gttctgatag ctatgattat
      841 ttttacaagt aatattgctc atgcgagtat tttcaattgg tttgatgaca caaaaagtca
      901 aacagagttt ttgaataatt ctctttatag accttaccta aaaaagtaca atggagattt
      961 ccttagtcag ttcgggattt ggctcggatg ggctgtggta aaaggcatgt ttactgtgac
     1021 agattcgatt caaaatatga ttccagatgt gctggatttg ttcaatttca ttgaaagtac
     1081 agggctaaat aatgtttatc aatcagccgt aaatacaatt gtcattggat taatgatcct
     1141 ttcattgatg tttgttggtt acaaaatgat tactggaaaa ggaacagtgg atttaaaatc
     1201 ggttggaatg aacgtagtga tgtctatagc attaatactt ttaatgccaa ccatgatttc
     1261 tagtggtatt caatttgcta aaacatttta taaggactct actacgatta caaattcaga
     1321 tgacggagtc gcttggtcat tgataaaaca aggagtaaca gatttagcat atattaataa
     1381 aacagatcag tactcaagca ttgataaaac ggaggatcga aataatttaa cacgaaaaaa
     1441 ttttcaacag acggatctca cacaagtgtt aacggataaa gtgattgata aattggaaaa
     1501 agaaaatcca attgcggaca atcttcgtta tgaattagtt gaaaattcta ataatgaatt
     1561 tgttgctaca aagttttctg acaacttctt atcaacattt tctgatagct tgaaatcagg
     1621 ctattaccga taccaagcaa atctatgggg gatttcaatt gggttgacag cgttagcaat
     1681 cgcctatgtg ttcagcgcgt ttgtgatcat tacagcaata ctagaacttg catttaaacg
     1741 tgtgttaggt gttcttgttt ttgcaacaga tattgagaca ggacagcgtt caaaagttgt
     1801 tctatcagat attctacaat gttacttaac tgttgggttt caaggatttg ggttatcaat
     1861 gtttgctatg tttatcaatt ttctgaactc aggacaaggt atctcaacaa atatttttat
     1921 aaaaacaatt gcttatatct gtgccgtttt tgttttaatc aaaggcagtg aaacagtcat
     1981 gagatacttt ggtgttgata ttggactcaa agaaggctat gggcaacttg cttctgcttt
     2041 tggaatgggc gcaatgctgt ttagaaaagg atcaaatgga tttaatcgtg ctaaaggaag
     2101 cggagaagga gaagatcgaa aaccagaaaa gaatttcggc gaaactctat caaaaaaagc
     2161 tggcaaaact ggaagagctt tgggatatgc ccatgaacgt ggattatccg gtttagcttc
     2221 agatggagct acaatggcta gtgaaagagc aacaaaacca tttaagagta tgcgtgatat
     2281 agctaatgat accaagaata agtttaaaga aggtttagat gatggtacag tgtccgctat
     2341 taataaaaat agcaaaccta tgttagctaa aaataaagaa gatgaaactg gaaaatatgc
     2401 tgattccgtg ccaacacgtc tttcagatag aaaagatggt gctaaagttg aaaatgccga
     2461 tagaattatg tctagttctg aacgtatgcg tgaagcaatg aaaaataatg ctgaatcgaa
     2521 taataatcct gcaagcgcaa ttcagcaaaa agtccaacaa gatattgaag aacgtaaaaa
     2581 tgctatgcac ggtcaagcta agtctgctga agaattaatt aatcagaagc gtcatgaagc
     2641 gaaatacacg cctgaagcaa tgaatcgcga ggaaataatg ttaaggaaac gtgtagaagg
     2701 gcggacaggc gctaatattg acggaaaaga agcgttgacc aaagaaagaa ttcaagaagc
     2761 taaaaattca aatacaggat tagaaaaatt agtcaaagaa aatccacaaa attctacaag
     2821 tagtcatggt ggccgatcag tagatgtccg agaaaatatt caaggttcac tgaatggtca
     2881 tgttggacgg aatgttgatg taagagagaa tttacaaagt tcttcaaatg ctcaaggtac
     2941 taagacaatt gatgttcgag aaaatctgca aagtagcagt acaagtcaac caaaaacagt
     3001 agatgttcga gaaaatgtac aaaattctac aagtagtcaa gggtttaaat cagtggatgt
     3061 ccgagagaat gtgcaaggta gcagtgcaag tcaaccaaaa acagtagatg tccgagaaaa
     3121 tgtacaaagt tctacaagta gtcaaggatt caaaacagta gatgttcggg agaacattca
     3181 aaaagatagt ggagaaatga aagaaaaaac acaaaaaatc aatatcgttg aagacagaaa
     3241 gtcagcgaaa aaatttgatt caaatcatga aacagtgatt attgatagtg aaatccgtga
     3301 aaatgataag ggatcaaaat caagacgaag attcacttat gaagacaatg agttattcag
     3361 agatacactt aatgatccta atccactttt cgataattta ttaagaaata aaaggggggg
     3421 attgtcatgc aattaattct aaagattagt acgcatgatt tcttagaact tgacgaatta
     3481 tgtgaatcag taaagaacgc aacaaatgaa aaaatcacgg tcactaatta tattgaactg
     3541 atgattcaag aagaattaaa gatgaaaaat gaaatagaga gcaatttcaa atgttgctct
     3601 ctagaaaaaa atgtgagggg ataatcaatg gatgataaac aaaaaatgta cgaaaaaaat
     3661 cgcaaacata gtgaggcctt aaattttcgt gtcgataaaa agacttatct tgaactgaca
     3721 ttgattgcaa aacaaagggg aatttctaga gcagacgttt gccgtgagcg gttaaaggga
     3781 ctcaaatatg aaccgccgtt gttagctgaa gatgataggg tagttttaac caaacagtta
     3841 aatcaaattg gtaacaattt gaatcaagcg gtaaaggcat taaatatcat tgctaaatat
     3901 tttagagaag aatctga
//
LOCUS       DAGCWU010000183         3896 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.188, whole genome shotgun sequence.
ACCESSION   DAGCWU010000183 DAGCWU010000000
VERSION     DAGCWU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.188"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(25..936)
                     /locus_tag="MBZ71_002518"
     CDS             complement(25..936)
                     /locus_tag="MBZ71_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBT4430417.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1393..1821)
                     /locus_tag="MBZ71_002519"
     CDS             complement(1393..1821)
                     /locus_tag="MBZ71_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430418.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1849..2487)
                     /locus_tag="MBZ71_002520"
     CDS             complement(1849..2487)
                     /locus_tag="MBZ71_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4430419.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2491..3261)
                     /locus_tag="MBZ71_002521"
     CDS             complement(2491..3261)
                     /locus_tag="MBZ71_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBT4430420.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT         1186 a          738 c          572 g         1400 t
ORIGIN      
        1 gttcatattt tttatttata ccgcttattt actacttgct gtactagaat ctgaagtgaa
       61 tgtactcaaa acattttcaa agtctttgtc tttgattttt acattagcgt ctttcaatac
      121 ttcgccaatt actttattct ggaaggtact gtcacttaat tttgtgtctg ttgcaatttc
      181 tttcaattct tttttgtatt tatccatgtc gttgcctttg ttttggtttt tcaccatttt
      241 aaccacataa tattccgtag tataagttga agcattggta gaagtgatca catcagaaac
      301 ttgaccatct ttcaacttaa aggctgcttc tttgacttct gccggaaccg ttgtagtagt
      361 ggaatcaaat ttcacttttc cgccatcttc ttttgtcgta gtatcagttg acttgtcttt
      421 ggctaattta gagaaatcgt ctcccttatc agcagctttc ttcacatctt ttgcatcatc
      481 ttcacttgct acttggataa tttgagcttc cacttctgga tggaaagatt cccaagccgt
      541 ttttaaatct tcatcggtca atttgatatg tttctttaga ccttcttgaa aggctagact
      601 ttgtttgata ctgtctttga atgtttgttc tgtgtaacct gcagatttca attgtgaatc
      661 aaatgaatca cccaattgtt tctttgtttg atcatattga gaatcgattt ctttggttga
      721 tacgtcatct ccatatttat cttcaaatac ttgataaacg atcaactgtt gcacagcgga
      781 atctgctcct tgcatttttt tcaagctatc cattacttct tgttcgctga ttgtaccgcc
      841 tttataacta acggctgttt tttcaccggc attcgtacag cctgacaata agccagtgcc
      901 tagtacgatg gcaagtgcta aaaatctttt tttcatttat caattctcct acttttcgtt
      961 ttttgcgaat tgctaaaaac aaaggtgttc aagatgaaaa tcatcaagaa cacctttact
     1021 aatctgctcg atacgtcctg aatagatata aggctacaca atccagaaag ttcgctcagc
     1081 agaataaatg tcaagtagca catcacacct taatatacta cttcttttgt taccgttttt
     1141 caagtctatc ttgcgttccg tatttaacgt aactaattct tagaacaaat gttatcgatt
     1201 ttgtttttct tcttccgctg ctgggatatt caaatcagac acttttatgt tcgattttcc
     1261 agacattttt ttgttgtttt tattatgata tatttttata caactaaaaa tgaatatatt
     1321 aaaactacca aaaataactt ttaacgctgt ttcattatat gttttttcta ttttatataa
     1381 acttttggat acttaatacc aaccggttgg attgtaccaa catgagaatg tgtcagttct
     1441 atttcctgtc gcacttgcat aggcccatac tccttttgtt gcatttgctg atcgaataga
     1501 gtttccaact ttcgcttttg caccgtgacg attatagaca cgactatagt ggttagaatg
     1561 acctgttttt ctcgtacaag ttgtatcact ataataaacc catcttccag agctatgatt
     1621 aattgctgtt ctagaaattt cccggtctac tggtaaattt gacttaataa tattaccttt
     1681 agcatcaaaa tgaatctctc ctggctttaa agatttgtaa tagatttcac cgtgaactga
     1741 atttgcttcc acttcagatg acatagcgtt tcctccaaca aaacctatcc ctagtgcaca
     1801 aataataact gcttttttca taaaacgaca tcctttcctt tttgattttt aaagcatgat
     1861 cttcttgttt cctattttgg tcagatatgg atcatgcgtg actacgataa ctaacgtacc
     1921 ttgggaagct tgttgcgata agatttcaac tactttatcc ttatttcttt tatccaatga
     1981 cccagtcggt tcatcggcta aaatgatttt gctattcttc aataagactc tcgcacaggc
     2041 gattctttgc ttctcaccac cagataatgt gtagataggc gtacttagct tcttgtttat
     2101 atttactttt ttcaatgcct catactttgc tgttcgctta tctgaaacat ttaaattcga
     2161 aaacttcaat ccaatatcta aattaaaatc gattgtttca tattcaatca acccaaaatc
     2221 ttgaaaaaga aaagatatgg tatttctttt tatcaagcgc ttctctctcc cagttaattg
     2281 tttcgtctct tgatcgaaca aatagaattg tcctgaactg aactgatcat tcaatcctaa
     2341 tatattcaat aacgtacttt tacctgctcc actttctcca acaacagtca caagatcccc
     2401 agcatccgca gtgaaacgat ccaaatgaag aatcgttttg tttcgaatga tttttttcgc
     2461 accttttatc actatcaacg gctccaattg ttaacctcca ttcatcagca ataactgact
     2521 tttcttttct gtagacaaaa taaaaacaac tgaaagaata aaatcaatga agctaaagaa
     2581 aaaaatatta aaaagatatt taggctctct ttctataaga aaaaggaaga ttacacttgt
     2641 cgttccccat ttgatgaggt tcatcatgaa gaaatcttta tacgtttcaa agagcgaata
     2701 accacttgaa cgaaagatag cgattttccg acaattgtag acaaaatata tgcataacat
     2761 gacatagctc aacaagaaga aagaaaagaa agtgagtaaa aatatctgta tcaagtaatg
     2821 aaatgatctg cttaacactt tgacagctat ctcttcagtc tgagtatacg gaaatgataa
     2881 ctgcaagtta tcacgtaact gactttcttc aatcagctca aataaccttt ctttcgacaa
     2941 agagcctatc ttgatcttta acggtggata gatttctccg ctgagaatat ttctttccaa
     3001 atacatactg tttttcaatg tcagtatttc aacaaccgga taatgttcga tccaggggat
     3061 agaaggaata aatgtataaa tgggttgatt gttttttata aatagaaatt tcggttcttt
     3121 ttcactgatt tcttgataat attctgttat ttgcgccagg tcatttttta aatctgtatg
     3181 tctatatcgt tcagggatca atataacagg gtgcgtttct ttttcggtta tttggatcaa
     3241 ccttccagag acatcaatca aaggaaattt cttaagataa ttcagattta tttttatccc
     3301 tctgccaaac tgttcatttt cttcattcaa gtaatctgat acattgacta atatacttcc
     3361 gtttttattc aaagtttcat ataattcctg atctgctgct gatgcaaaac gattggaata
     3421 aataaaatcc acgtggtttt tcccgatggt tactggataa aatacatgat agtcctcacc
     3481 agcgatttct tttggtggtt catgggaaac agctaactgt gaagaagaaa aaacgatagt
     3541 tgctaattcg acattggcaa cggtcaaaag aagagaaaac aacaatccaa atgtaaaaag
     3601 aatgtattgg atatagttga acgcttctct taacgaagcg attttttcaa ctaataaaag
     3661 agaaatcaaa gagataccat atattccgct gaacaagaaa acgtacatct ggaacaaata
     3721 aagaaattgc tccttataat acataaaaat aaaaccacca atgattcctg caattatgaa
     3781 tgtaataatg attttctttt tccccaataa acaccacaat acttgatgta atgaaaagtc
     3841 attattcaac aataaactga tttctttatt catcaataag aaaaaggatg ataaca
//
LOCUS       DAGCWU010000184         3874 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.189, whole genome shotgun sequence.
ACCESSION   DAGCWU010000184 DAGCWU010000000
VERSION     DAGCWU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.189"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(7..528)
                     /locus_tag="MBZ71_002522"
     CDS             complement(7..528)
                     /locus_tag="MBZ71_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBT4430421.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            777..2399
                     /locus_tag="MBZ71_002523"
     CDS             777..2399
                     /locus_tag="MBZ71_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4430422.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            complement(2528..3391)
                     /locus_tag="MBZ71_002524"
     CDS             complement(2528..3391)
                     /locus_tag="MBZ71_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4430423.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
BASE COUNT         1260 a          740 c          697 g         1177 t
ORIGIN      
        1 ttttacttat ctaaatgcat agacgatatg tgtgatacca aataacataa aatagaatcc
       61 aactaaaaag cttaatgtca acgcagaaga aattggatta aacaataaga aaattcctaa
      121 taaaattcct aaaatattga cgatgatcgt aaaccaataa tatccttcac ttactcctct
      181 tgccaagtca gcagtaaata acgctaaaac tgaatcagca ataaaccata ctgcaaaaac
      241 aaaaggcaat gctgcgactc ctgcaccaat attgaataag aaaaagacgc caactagaat
      301 atcgatgatc ccgattatta agggcatctt tcctttatat cctgttagct cttttaatct
      361 gttccgtaca aacagttcaa atatcccttt aataaaggca aaaatcgcaa agactactac
      421 aattgcaact aaatttccga cagggtcttg aaatgataac aatgagacaa gtacaaataa
      481 aattcccaaa atcaatgagc cccagtcgat ggttcttttt tcatccattt tcaatccttc
      541 tttcatttct tttacagttt tatagtactc cctcttataa ataacgtcaa aaattatgct
      601 gttgcaaact aataaatcct atagtagcta ttttcgctcc gcttttattt ttattttttt
      661 cattctgttt cacgtgaaac acaaagaatg ttcgattgcc ttgaatcttt tacctttccc
      721 ctatataatt ctataagact tgtcaaaact aataatgata aaagtgagga aaaaaattga
      781 ttactgtaaa tgatgtgagt ttgcactttt cagaccgcaa actatttgat gatgtgaata
      841 ttaaatttac ccccggaaac tgctatggct tgattggtgc aaatggtgca ggtaaatcaa
      901 cgttccttaa agtgttatca ggggatctgc agccttccac aggttccgtt actatgggac
      961 cagatgaacg gatggccgta ttgaaacaga accattacga ctatgaagac tatactgtac
     1021 ttgaaacagt catcatggga cacaagcgtc tgtatgaagt gctgaaagaa aaagatgcga
     1081 tttacatgaa agaggatttc actgacgaag atggtattcg agcagctgaa ttagaaggag
     1141 aatttgctga gcttaatgga tgggaagctg aaccagaagc agctgtttta ctgcaaggtc
     1201 tgaacatccc tgaagaattg caccatcagc aaatgagtga attaactggt ggacaaaagg
     1261 taaaagtgtt attagcacaa acactatttg gtaaacctga tgttcttttg ctagacgaac
     1321 caacaaacgg tctggatatc caatcgatca attggctgga agaatttttg atcaattttg
     1381 aaaatactgt gatcgttgta tcccatgacc gtcacttttt gaacaaagta tgtacacata
     1441 tggcggatct agattttggt aaaatcaaac tttatgtagg aaattatgat ttttggttgg
     1501 aatctagtca actagcggct aaacttcaag caaacgcaaa tgccaaaaaa gaagaacaaa
     1561 tcaaagaact ccaagaattt attgcccgct ttagtgcgaa tgcatcaaaa tcaaaacaag
     1621 caacttcacg taaaaaaatg cttgaaaaaa ttactttaga cgatatccaa ccttcttcta
     1681 gacgttatcc attcgttgga ttcaaacctg aacgtgagat tggaaacgac ttattgcagg
     1741 tagaaaatgt aagtgtaacg attgatggaa agaaaatttt agatgatata tcttttaatt
     1801 tgagcaaaga agacaaggtc gctttcgtcg cagaaaatga tataacaaca acaacgctat
     1861 tcaaagttat catgggtgaa attacgccag atactggaac agtacgttgg ggtgtgacaa
     1921 caagtaattc ttatttacca aaggatacaa cgaaagaatt cgatactgat ctaacgattt
     1981 tagactggtt gcgtcaatat gcaagtaaag aagaagatga taacaccttc ttaagaagct
     2041 tcttaggaag aatgttgttt tctggtgaag aagtattgaa accagtgaat gttttatctg
     2101 gtggagagaa agtccgttgc atgctttcaa aaatgatgct ttcgaaagct aacgtacttg
     2161 tattagatga tccaacgaac catttagact tagaatcaat cacagcattg aatgacggct
     2221 tgatggcatt tactggctca atcttatttg cgtcacatga ccatcaattt atccaaacat
     2281 tggcaaaccg catcattgcc gtttcggata aaggtgtgat cgatcgagct gatacgacat
     2341 atgatgaatt tttggaaaat gcagatattc aaaaacaatt gaacgagtta tgggcataat
     2401 ggcctattta gcttaaaaaa taaaaaaaca gcgacctcta agagatagga tgctcatcta
     2461 tcttttaaga gtcgctgttt ttgttacaat caatttctgg gcttttttac agaatgagtt
     2521 actgctttta tcgactgatt cccatcgaaa aaatcgcgat acagccatag cctgtgtgac
     2581 tggcgatcgt tggtcctaag ggataaagct tcacatcttt catctttatc tgctcttcca
     2641 aaagccgttt tgccttctcc gcgctctcta gatccccagc gtaagccaca taaacgattt
     2701 gatcaagtgg tgcaacgatt gtttttaccg tctcattgac gattgtctgg atcgacttgt
     2761 ttcttcctcg aactttgtca acattcgtca gccttccttc tttatccacc gtaataatag
     2821 gctttacact caatagactg ccaagagtag cacttgcttt ggaaatccta ccgcctcgtt
     2881 ctagatattt cagatcgtct actgttaccc atgaatggac tttcaaataa ttactttcca
     2941 gccactctag ggtttcttcg atgcttttac cttctttttt caatcttaca gcttccaata
     3001 ctaaaagccc ttccccaata cttgcagctt ttgtatcaaa aacatagatc tcagggtctt
     3061 catattcttc cttcagcaat tcaaccgctt gaagcgcact actgtaagag ccgcttaatc
     3121 cactggaaaa agccacatat aaaacaggaa tctgtttttc cgcataagaa cggaaaaatt
     3181 ctaaatatcg gccaacattg atttgtgatg tcgtaggcat cgcaccattt ttcagctgat
     3241 tcatgtagcg gtcataattg aatgtctttc cgaggtcatc tactaactct tgatcattca
     3301 attggagatt cattgataaa aaatcaactt tttcttttgc taatacatgg tatggcagat
     3361 cacaacaaga atctgttaca atttgataca catttttcaa ccccttctcg ttacatttta
     3421 cccttatcgt ctattatagc aaatctcttg cgctagacac actttaaccg gtttattttc
     3481 gcaagttcta atcaaaataa gaggttgtga cagaagtggt cagcttcaag aaataagaag
     3541 gaatttacga aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgtagaagct
     3601 acttctggaa caccgtgaat cagaggttgt gaaatcagcg ttttgacctg agtattagag
     3661 aaaaaaacgg aaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     3721 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttagctc cgagtcacaa
     3781 ggaacaattt caaaaaacag cttctcatgt ttgttgaata ttgtgacttg tgaccgagga
     3841 gttggcagga gaacaccgtt tagtggagag gttg
//
LOCUS       DAGCWU010000185         3793 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.190, whole genome shotgun sequence.
ACCESSION   DAGCWU010000185 DAGCWU010000000
VERSION     DAGCWU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.190"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(53..787)
                     /locus_tag="MBZ71_002525"
     CDS             complement(53..787)
                     /locus_tag="MBZ71_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBT4430424.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="MBZ71_002526"
     CDS             1114..1524
                     /locus_tag="MBZ71_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBT4430425.1"
                     /translation="MNFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="MBZ71_002527"
     CDS             1517..2218
                     /locus_tag="MBZ71_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBT4430426.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..>3793
                     /locus_tag="MBZ71_002528"
     CDS             2218..>3793
                     /locus_tag="MBZ71_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4430427.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKL"
BASE COUNT         1176 a          688 c          743 g         1186 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaatt tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tat
//
LOCUS       DAGCWU010000186         3785 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.191, whole genome shotgun sequence.
ACCESSION   DAGCWU010000186 DAGCWU010000000
VERSION     DAGCWU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.191"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..171
                     /locus_tag="MBZ71_002529"
     CDS             <1..171
                     /locus_tag="MBZ71_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4430428.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="MBZ71_002530"
     CDS             406..642
                     /locus_tag="MBZ71_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430429.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="MBZ71_002531"
     CDS             649..2601
                     /locus_tag="MBZ71_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBT4430430.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="MBZ71_002532"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="MBZ71_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBT4430431.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="MBZ71_002533"
     CDS             3400..>3785
                     /locus_tag="MBZ71_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4430432.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DAGCWU010000187         3781 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.192, whole genome shotgun sequence.
ACCESSION   DAGCWU010000187 DAGCWU010000000
VERSION     DAGCWU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.192"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..945
                     /locus_tag="MBZ71_002534"
     CDS             <1..945
                     /locus_tag="MBZ71_002534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430433.1"
                     /translation="SSLIFAVNPIFLNIYVKKYYSIDTKIAPDNSAISQRWHAFAQEI
                     AVMINNNTDIVVLTIFSNVLEISVYVIYNMVLSGVKQLLQTFTTGIGAAFGSMIAEGI
                     DNSSLNRKLKVFEVLVYNITTILFSVTLVTIVPFVRVYTSNINDVNYIRPVFAIIITI
                     AAMFNCFRIPYQTIVYAAGHFKQTRNGSIVEVILNLSISIILVIKIGLIGVAIGTLVG
                     SIFRTLQYGIYLSRNIIVRSNWLFYTKIVISLLETMIIYIISSIFINISNVSYFGWIS
                     YAINIFMLSIAIVCMGSFLFYRKDTISIIKRLIFALKR"
     gene            complement(1243..1803)
                     /locus_tag="MBZ71_002535"
                     /pseudo
     CDS             complement(1243..1803)
                     /locus_tag="MBZ71_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369693.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2037..3329
                     /locus_tag="MBZ71_002536"
     CDS             2037..3329
                     /locus_tag="MBZ71_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4430434.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCLISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYIRRLWRPGFKAHLTETDIVDRLLKRSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            3587..>3781
                     /locus_tag="MBZ71_002537"
     CDS             3587..>3781
                     /locus_tag="MBZ71_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4430435.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
BASE COUNT         1264 a          610 c          648 g         1259 t
ORIGIN      
        1 agttcattaa tttttgctgt taatcctatt tttttgaata tttatgttaa gaaatattac
       61 agcatagata caaaaatagc accggataat tcagcgattt cgcaaaggtg gcacgctttt
      121 gcacaagaaa ttgcagttat gataaataac aatacggata ttgtcgttct tactatattc
      181 tcaaatgtat tagaaatttc tgtatacgta atttataaca tggttttaag tggagttaag
      241 cagttattac agacatttac tacaggtata ggggcagcat ttggaagcat gattgcggaa
      301 ggaatagata atagtagctt aaatcgtaag cttaaagttt ttgaagtact cgtttataat
      361 ataactacta ttttattttc tgtaacatta gttacaattg taccatttgt tagagtttat
      421 acttcaaaca taaatgatgt aaattatata cgtccggtat tcgcaattat tattacaatt
      481 gctgctatgt ttaattgttt tcgaattcct tatcaaacga ttgtatatgc ggcaggacat
      541 ttcaaacaaa caagaaatgg ttctatagtt gaagttattc taaatctttc gatttctata
      601 attctagtta ttaaaatagg actaataggc gttgcaatag gtacattagt gggttctata
      661 tttagaacgc tacaatatgg tatatattta tcgagaaata taatagtgcg ctctaattgg
      721 ttattttata caaaaattgt tatttctctt ctagagacaa tgattattta tataataagc
      781 tcaatattta taaatataag taatgtttcg tattttggtt ggattagtta tgcgataaat
      841 atatttatgt tatctattgc tattgtatgc atgggtagtt tcctatttta taggaaagat
      901 actatttcaa tcataaagag attaatattt gctttgaaaa gatgatataa aaagtatttt
      961 taatttgaga atatctattg cgactattag actattaatt tggttgagga ttagatagtc
     1021 gtgaaatgtt tattctatta cattagattt tttagtaata actatataaa ttatctaaat
     1081 taagatttaa atgatagctt atagaaaata accttatttt agataatatt tctattaatt
     1141 atgtttaatt gattgcattt tgtagagagt gggacaatag tcctttctcc aaaagaaaac
     1201 aagtccgatt ttgatggtta atttcaaaat cggacttgtt ttttcttctt ttaaataagg
     1261 caagatttct tctttataaa gggcataata gaattcgttt gattgcttat ccaaagcagt
     1321 ttcataacga ttcgtttctt tacgccagaa gaaactgaac ttgttggcat ctgcttcaat
     1381 ttttgtacca tcaataaata ttgcttcatt atcaattact taattggtga tcgattggcg
     1441 gctaaataag acaaacgctt cagctaaaag gtcagcgggt gtttcctgac ttcggaatcg
     1501 attgatggtc cgatagctga cttgttcgtg gttagccaac caacacatac gatagctgtc
     1561 atctaataga aattcaattt tccgaccaga aaagacacgc tgcatataag catatagaat
     1621 aatttttaac atcattttgg ggtggtaggc cggtcgtcct agttattctt ataattcaag
     1681 aaagagtgtt tgtggcattg ctacgactaa agcgttgact gcaaaagcga catctgtttc
     1741 aggaatatat tgttccatat ctaatggtaa agttgtttga ttgatgggat aatttttaaa
     1801 cataaagtac tcgcttcctt tctgattggg cttggtcact ttaatcttac agggatgagt
     1861 gctttttgtg taccttaaaa tcaaaaaaag tggggcaata atcagatttt ggctctatac
     1921 tttttgatgt tggtggatga caccagcatc ttttttattt tccaaaacat atagaaaaga
     1981 caccttgaaa tcctttagaa ttaatttggc gaaccaaccc aaaaggagga atcaagatgt
     2041 cttacactca ccttatcaaa gaaacattag atattctaga cttatcagtt acttttaacg
     2101 aaaattgtct cacaaaagaa aaatacaaag ggcaaatctg tcatatttat cgtggaaact
     2161 tgatttatac agcccaagaa tgcatccact gtaaacacca gattgcttca gatattgtgc
     2221 gttggggtac aacaaccgtc cggttgttaa tgaacgatgt ttctgaatac cgtacctacc
     2281 tcgaattgaa aaagcaacgc ttcaaatgca aagcttgtca gcggaccttt gtcgcagata
     2341 cttctgttgc gaaaaaacat tgtctcatca gtgagaaagt ccgctggtct gtcgtgactc
     2401 gtctgaaaaa aaatacctca atgacagaga tcgccgctca gaaaaatcta tcggtctctt
     2461 ctgtttaccg tattatgaag cgattttatc gtccgttgaa tccttttcgg acaccgttac
     2521 ctaaggtact ttgttttgat gaattcaaat cagttcgggg tgtttcaggt gctatgagtt
     2581 tcatcatgat ggatggacaa acacagcgct tattggacat tgtcgagaac cgacaactgc
     2641 cttttttaaa acgttatttt agtcattttt cacgagaaat tcgtgaagcg gtcgaatgga
     2701 tcgtgattga tatgtacgct ccttatgtat cgctcgttaa aaagctgttt ccaaaagcac
     2761 agctgattat tgaccggttt cacatcgtcc aacatattgg tcgtaccttt cgcaatcatc
     2821 gaatcaaaga aacgaaccaa ctattaaaaa gcaaagaaca aaaacactat caattaggta
     2881 agcaattaaa aagatactgg aaacttcttc aaaaagatga acgcaagctt gattatattc
     2941 gacgcctttg gcgacctggc ttcaaagctc atttaacaga gaccgatatt gtggatcgct
     3001 tactgaaaag gagccctgct ttgcgtgtag gctatcagtt atatcaagac tttctttacg
     3061 ctgtaaagga acgagactac gtatcgtttg aagaactact taccaataat atcatgcttc
     3121 ccgaaggcta tcagacgacc ttgagaacgt tccagaaatt tctgccacaa atcaaaaatg
     3181 ctttacaaca aagttattca aatggcccac ttgaatgtct gaacaaccat atcaaagtgt
     3241 taaaacgaaa tgcatatggc tttcgtagct tttataattt caaattgcgc atcatgattc
     3301 gtcatggcaa cgcattgatt ttcaactaaa aaagatcaag aggaactgat ccacttgatc
     3361 tccaattatt ctaagatttc tctttaccaa catcatttga caaagagcca cataaaatag
     3421 caaataatat cctaaaacag ctatgatttt ttccttacaa agtatacatt tctgattgaa
     3481 tttatgataa agtccgtata attgtgtaaa agtaaaaagg ccatataaca gtccttttac
     3541 ggtacaatgt ttttaacgac aaaaacatac ccaggaggac ttttacatga cccaagtaca
     3601 ttttacactg aaaagcgaag agattcaaag cattattgaa tattctgtaa aggatgacgt
     3661 ttctaaaaat attttaacaa cggtatttaa tcaactaatg gaaaatcaac gaacagaata
     3721 tattcaagca aaagaatatg aacgaacaga aaaccgacaa agtcaacgaa atggctatta
     3781 t
//
LOCUS       DAGCWU010000188         3732 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.193, whole genome shotgun sequence.
ACCESSION   DAGCWU010000188 DAGCWU010000000
VERSION     DAGCWU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.193"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..312)
                     /locus_tag="MBZ71_002538"
     CDS             complement(<1..312)
                     /locus_tag="MBZ71_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430436.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YE"
     gene            434..1018
                     /locus_tag="MBZ71_002539"
     CDS             434..1018
                     /locus_tag="MBZ71_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430437.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            1029..1274
                     /locus_tag="MBZ71_002540"
     CDS             1029..1274
                     /locus_tag="MBZ71_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430438.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            1280..2002
                     /locus_tag="MBZ71_002541"
     CDS             1280..2002
                     /locus_tag="MBZ71_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430439.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            2133..2876
                     /locus_tag="MBZ71_002542"
     CDS             2133..2876
                     /locus_tag="MBZ71_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430440.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2882..3247
                     /locus_tag="MBZ71_002543"
     CDS             2882..3247
                     /locus_tag="MBZ71_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430441.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            3263..>3732
                     /locus_tag="MBZ71_002544"
     CDS             3263..>3732
                     /locus_tag="MBZ71_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430442.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFM"
BASE COUNT         1454 a          498 c          657 g         1123 t
ORIGIN      
        1 ctcatattca tagctatcaa ttagctcagt tgaactataa gctttttcta gtagttccat
       61 ttcccatttt ggagagtttg gatcactttt tcttagtcta gctatatctt ctaaaatttt
      121 cttagaagta ttatcctcgt aaatatttgc taaatcagat aatttatcaa ttaaatctaa
      181 aataagcgca ctgtaattga cagctattgc agaacgataa tttccctgat aaaaactact
      241 tctaacttct tctaataatg gaataattct aggattttta acttttttta tttcatcatc
      301 taatataaac atataaatct ccttttttta tttgtatgac aagtttacca taaaaaaaaa
      361 ttaaaaagcg agacatattt atgttgttgt gttatcataa taacataaat atgtagagtg
      421 agatgatgaa aaaatggatt tgaaacaatc gttttacatc tggttatatc ctgataatcc
      481 tatgtcaata atggctgata tttttgtagt tagcatctta ttgtatttat tttatctaat
      541 tttctttcac aatttaatcg ggtacatgat tcttgattgg aaaggaatgt tcgatataga
      601 aagaaaacca tttgctaaca atcaaaccta taccgtcttc gaaagtggag attgggaaga
      661 aaatgaagtt gatgtgatcc atgaaattgg attagcttta tcaggaatac aagatgctga
      721 tgttttggta tcaatgacaa cggatactgg atacaacgct tatgaggtat tttatcttat
      781 gtattttata tggcgtgatg gtcaagaaat tatcaatggt cgtgctgaaa aaacaatcaa
      841 ttctaatact gaaatttctt tttgtatggg tgaaggggaa atgattgtat ataaagatgg
      901 gtcagcaaac attaaaacag cctttggtga tattgaaaca agtattaagg aacttaggga
      961 atatgtgcag aacccatata attttcaata tttagcagga agaataaatt tagtttagga
     1021 gtaaaaatat gaaaaaaatt ataaatcaac caacaaatca cttgataatt gaaggtgtta
     1081 ttttcgtaac aatgtatttt actgtaggaa atggcatctc aaatacaatc attcaaaaaa
     1141 atccacaata cctattattt agtctttatg cttggattgt acaaatggtt tgggtttcca
     1201 tattgatgct gttgttttat tggatagtac gggttattct tttattgtta aacaaaagaa
     1261 aaaaggatag gtgaataaaa tgaaatcaac taaattgaag gttgtattgt tatcgacagc
     1321 agcattaata acccttacag catgtggtac aactaatagt gctaataata acaaagacac
     1381 aaagtctagt agtacgatgg ttactaaaaa cagcactaca tcggcatcaa caacaatgtc
     1441 ttcaatgact gaaaataatg ctgaaaagta tcaaaaatac gaagacatta ataaaggtca
     1501 aaagccggat gaagtttata acaactttat taatacagta aaaaatgatg aaaattatgg
     1561 gaatgatcca ttgcaaaata aaaatctgac tatcgttctt tacaaacctg attgtccaga
     1621 ttgtcaaaaa gcagaatcaa aaatcatgca agaaataaaa gaaggattta gttatgctcc
     1681 aacattaatg catgatatta ccataaataa accagcttac gagtattacg cagttgctgt
     1741 agatattact gatggtgtac ctgagtgggt acaaaacatt cctggtctgg ctgatgagaa
     1801 caatcaatat catacgccaa cagttgcagt agtaacacca aagaaagtta acggcacact
     1861 atactgggat gtaatgggaa tgacaacaaa tacaactgat gaaatgattg aaagtacaac
     1921 taaatattat atggatttgg gagcatataa attatatcaa caatcagagc ctgttatgga
     1981 cgatcaatcc aaagttaaat agatctttag ggcgtaagcc ctattttttt aaaattaagt
     2041 gataacaaat aaatgatttc tgaaacaaac gttcttactc aactactcga aacacacaag
     2101 ctaattaact agattataaa ggagaatgcg taatgaaaga aatcagtgca aatgaagctc
     2161 atattccgca aaagaatcag gttggtatat ataaggtgct tcatggtgaa ggaatttata
     2221 aaatacagtt acttaccggt tacaataatg gtgcaaatgg gaagtggtta aaggcaacaa
     2281 gtattcgtga acttagaagt gaagtagaaa aaattaggct tggtctcaca agtgaagaaa
     2341 tagctaaact gaagggaaat aattcttttg taattgaaaa agaaaaggaa aaacatctac
     2401 tttatcgtga gctgaaaatt gaagaatact atgaaattga tttacctgaa aaattaagaa
     2461 ttattgatca acgttcagaa gaaaaatgga aaactgaaat atatccaagc ttaaatgact
     2521 acaagacatc aggaaagccc atgtataact acacaggctt tttaacctac acacaataca
     2581 aatatgcctt tcgcaaatta aaaggcatta aacaagctga aattgctctg tatgagatga
     2641 aaaaagtgga tagattgaaa ttgattcaag atttagtgca aaatacatgg aatcaagcga
     2701 ttgtcgagct agaaaaagaa aatgggaaaa ttttaactaa taaatatctg atcgtaaatg
     2761 aaattgaaga attaattggt ttattttcat gtgaaaaagg tgaagtcttc ttgaagtcat
     2821 caccaaccta ttcaattttg gacagtccgt atcagtttat acaggcaagg agatgagtta
     2881 gatgtctagc ttgaaagatg actatatcca agcaaaacaa gagtatgaaa aaatacagat
     2941 gttgaaatct gagaaagtcg aattattttt aacacaaaaa ttagcagagg atcatattgc
     3001 taacttagat gctaaaactt tagaggaata tgatcggcag tttaatcaga tacatcacaa
     3061 tatcattttg cttgttgagc aggcaaagcg aaggtttgaa aaagcggaat atgatctatt
     3121 agatcaagga attgtatgct tggataaggg attgcgaaga tattatggtg cagagttaaa
     3181 aaagcaaaat gtagatagaa atgcactaaa acatgaaatc ttagaattga taatgcaacg
     3241 aatttgaagg gatagaaagc aaatgtcaca aaacattagt gctgtaaaac aattaataga
     3301 agaagattta gagaccacag ttcagagatt agcggaacga atccattttg caaattatca
     3361 aaccatctct aactggtcaa gcaggaacag aaatccggaa gaaattccaa tgaaattttt
     3421 ttatcgaatc ggaaaagaat atggtgttga tccgataaaa attttcgaga agaccatgat
     3481 ttattactat catggaacac ctaacgaagt gttagaacat cttttggaag aggcaaaaga
     3541 aaaatcatca atattagagc tatcgcaaaa tgacaaagtt attcaagtga aatgcgttga
     3601 tccaacagga aagattattg tcggttttga tattgaaaga aaatccaaaa tagattttat
     3661 tacacaatta aaaaagataa ataatttaga tattctaacg cttagaccag atcatgaaca
     3721 gttgtttatg ag
//
LOCUS       DAGCWU010000189         3709 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.194, whole genome shotgun sequence.
ACCESSION   DAGCWU010000189 DAGCWU010000000
VERSION     DAGCWU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.194"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            507..713
                     /locus_tag="MBZ71_002545"
     CDS             507..713
                     /locus_tag="MBZ71_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBT4430443.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            713..937
                     /locus_tag="MBZ71_002546"
     CDS             713..937
                     /locus_tag="MBZ71_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430444.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            952..1389
                     /locus_tag="MBZ71_002547"
     CDS             952..1389
                     /locus_tag="MBZ71_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430445.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            1382..2089
                     /locus_tag="MBZ71_002548"
     CDS             1382..2089
                     /locus_tag="MBZ71_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430446.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            2470..2649
                     /locus_tag="MBZ71_002549"
     CDS             2470..2649
                     /locus_tag="MBZ71_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430447.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            2742..2927
                     /locus_tag="MBZ71_002550"
     CDS             2742..2927
                     /locus_tag="MBZ71_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBT4430448.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            2961..3392
                     /locus_tag="MBZ71_002551"
     CDS             2961..3392
                     /locus_tag="MBZ71_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBT4430449.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         1313 a          614 c          763 g         1019 t
ORIGIN      
        1 gttattgcta gcatgtatgt tagaaatccc gatcatttta cacgatcgat gggcatataa
       61 ataaaagaag agttgtagtc ctaaatgagg attgcagctc tttttgtttt acatagaaac
      121 gggaacagga aaaggcctgt ttaggaaggc acaagcgcct tctcaattaa aaaacctttc
      181 tctcactcca accgccaccg ccgttcgaga agaaaggaag tgaacgctgc acgttcacta
      241 agatatggtg tcttgcaaag caagacaaaa taggaaaagc ttcctctcac caaaaggttc
      301 gttcctgttc gctatctctt tattgatttg ccagaccggc aacaagttgc cttttatcac
      361 caaaaagcga caaggacaag ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt
      421 cgttgattca ttctggccaa caaatcaata aagaaagaac taacgaaagg aagtaaaacc
      481 tttcctaaga ggaggcagaa tcaatcatgc gaaagaaatt tgattattgg ggtgttccct
      541 tcagcgaatt attcccaaac tatcatgcac cacatactgt cgaatgcgat tgtggagaaa
      601 gagcgaaatg tatcaaaagc tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata
      661 ccttgtctta tggcgattac attttgattg atgagaaagg aaagaaacgc tgatgaaaac
      721 acaacacaaa ttattgaatg taaaagaagt catttctgaa aaagtagcag aagctttact
      781 tattccgcgg tactggacga tagaaacccc tttagtagag gacgcaccga ctatgctatt
      841 tgaagaccta gtgaagattg caggatatga actgcattat caagaaaact tggaaactta
      901 tgaaaacgaa tattggttaa ccaaattaga gaactaaaaa ggagaaaaaa tatggctcag
      961 aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa aatggaggat
     1021 atgacgttcc tataccatga aacacgtgta cccaaaaaac attacaagga aaaattgtca
     1081 gtcgcagtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc gacgtactat
     1141 cgaacattgg aagaactgaa aaaacaaaat ggaaaatggt ttttccaagc cttactatgc
     1201 ttggaagcag gcgtcaaacc aagcacaatc aaaccttctg aataccaagc tttagaacta
     1261 acgtatggaa aatttatcga aacgaaaaaa gccaagactg tttcaagtga atggttggac
     1321 tactttgaaa atgtcaacaa gtatggcgca tgttacatca tgaaaaagga ggacaacgaa
     1381 aatgagtaat gtggtttatc aactaacagt ggaagatgtt tctcactttt taacaaacaa
     1441 agaacatttg aatgaagtag tcagacggat tcaaaaagac tttagttgta tggaagaaat
     1501 gaagtacttt attcaaagag tggaagaaca gttgtatatg gaaagcgaaa cgagtcaaac
     1561 gaaggtcaca gtcagccata atcatttaga cgtgtataaa cgtcgtcctg aaacggtaaa
     1621 aaaaggaaca gagcttctac gactagaaac agaaaaatac atcatgaatg tgtacctaga
     1681 aaggagtgag ctggtcgaaa actttgtcag ctacacgatt tgtaggaaac actattactc
     1741 agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag aaatggaaga
     1801 gctaaacagc aaaaatgcga cggtagacga ttattacctt gtatttacta gatttctgac
     1861 gtgtgggacg gaatctatcg tgttaatggg aatggattat gaactcgctg tacccgaagc
     1921 atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt acacgacttt
     1981 aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa atgagcttga
     2041 ctgtaaacga atgccatatc aacgatttga aaggaaaaaa ggtcaataac catttatacc
     2101 gaaaagtgag aatgtgtgaa ataagaacaa aaaaccttga aattaaggga atggttctta
     2161 tttcgcacca atcgctccaa ccgcccccgc cgctcaggtg gtgcgaaata agcgggcaac
     2221 gaccgataac ctaagagaaa gaaggaaatc catatgaata aaatcattcc tctgttgttc
     2281 gtactgtttg ttggcgagat acttatgcgt catgctgcag ctgtacattt aaacttcgta
     2341 gtattcttag gaatactggt attttgtttg atcttttggc gatttgtatt agcccttgtg
     2401 agtctactat tcaagatgat tgcagccata tttttcatag ggttatttcg atgaaaggag
     2461 agagtactaa tgccaattgc aataggaaat aaacgtttac ccgtgacgtt ggatgaaaag
     2521 agacaaaaag aattgcagga actcaaacag aagtacggca aaagtgaatc caggattatg
     2581 tgtattgcgt tagatttatt gattgcacaa gaaaaagcag ggtttgatgt accagcactc
     2641 aaaaagtgac gtcacttttc atttgaagaa acaaaagtga tagcacttta cgcacgtgtc
     2701 gatacgtgtt atgattattt tgtaaggagg tatagtgcac aatgccactt acgggaaaag
     2761 aaatgcttaa gttattgaaa aaaaatggct gggttgagcg aaggcaagag ggatctcatc
     2821 atcatcttta taaggatggt gttagaatca ctgttcctgt tcatgccaat caagatcttg
     2881 gccgaggact tgagcaaaaa attttaaagg atgcgggatt gaaataatct cgcattcgat
     2941 taaagaaagg attgactgaa atgttaagtg agcctttagc aaaaacctat cctgctattt
     3001 ttagcccaga agaaggtggt gggtacttta ttgaatttcc tgatgttcaa ggtgcttaca
     3061 caggaattaa cgaagacgat atatcttatg gaattgctat ggcacaagag gtgttaggta
     3121 tggttttagc agattatatt gagcatgaag atctacttcc agaaccaaca ccaatcaata
     3181 agatttctgt tgaagatgat tcttttacta ctttaattcg tgtagatgtt gccaaatatt
     3241 tgaaagacac agaacttgtc aaaaaaacct taacaattcc tcaatgggcg gataagctag
     3301 gcaaaagggc tggcataaat ttttcagtcc ttctaacaga atcaattgca gataaagcag
     3361 ataacattct tcgttctgga agaaataatt agaactcaaa agagtcagaa acttaaaaat
     3421 ctttggcttc tttttttact acaaagatgg cttctttaaa ttatttgcct gattgattta
     3481 aaaaaattgc gcaaagacta cgagctaatt aagttgctcg tggtctttct ttatgaaaac
     3541 tttttagatc tagataaaaa cattgagaca ctttgaaaaa tttttttaat agtaatgggc
     3601 gcttctatgt caagctgatt cttctttcgg taaattaatt ttacttagaa tatcactatc
     3661 ttgataaaaa atggtgtatt cacaaattct ggcataaaaa atacctact
//
LOCUS       DAGCWU010000190         3688 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.195, whole genome shotgun sequence.
ACCESSION   DAGCWU010000190 DAGCWU010000000
VERSION     DAGCWU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.195"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..276)
                     /locus_tag="MBZ71_002552"
     CDS             complement(<1..276)
                     /locus_tag="MBZ71_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4430450.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
     gene            611..2002
                     /locus_tag="MBZ71_002553"
     CDS             611..2002
                     /locus_tag="MBZ71_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBT4430451.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVTPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            2040..2870
                     /locus_tag="MBZ71_002554"
     CDS             2040..2870
                     /locus_tag="MBZ71_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4430452.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2842..>3688
                     /locus_tag="MBZ71_002555"
     CDS             2842..>3688
                     /locus_tag="MBZ71_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4430453.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYN"
BASE COUNT         1261 a          531 c          700 g         1196 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgttatatga atattgtaac ttgtgaataa ggaattagca ggagaatact
      421 agttcagcat aaaggaagtg atgggacttc tgagacaact ttctgttatg cagaaataca
      481 catataaaca gacaataaac gcttttcccc gtttctttta atttccttta caatttggtt
      541 attcactagg tttttaaact ttgctatggt aagatgatag aggcattatt ttatggttag
      601 gagtgtatat atgaataaaa atggggagtg gaatgcggca cgcagaatat tcattattct
      661 tatggatgta cttgtattaa atgtatcgat ctatcttagt tttttgctga aattccaggg
      721 agaagtacca gtgagaaact tagaaacctt tcagcattca gcaattttta tttccatcat
      781 ctttattgcg ttgaatattt tgttaggagc ttatgtattc tataaccgaa tgatcagtga
      841 tattgtcttc attactgtta ttggacaagt actaatagca ttggggatca tggtagttac
      901 ttttgctggc cgatggtttg catttccgcg tggcgtcatt ttgatctcgt ttatcctagg
      961 tacgatatta cttagtgttt accggatagt cgtttacaaa ctgtacttaa gagtcagtcg
     1021 tgataagaaa gtccttatcg tgggtttgga gaaagatgtc acaccagcta tccaaaattt
     1081 tcaatctaag aaaaacaata aacataaggt tgaagcagtg gcaatcgata attattacga
     1141 aaatacaaaa aaattgatcg atgatatgga tatcgtctat ttggcttcta aaatcccaga
     1201 agatgaaaaa atcaacattt atcaattagt gactaaaaaa gagaagaaat tatttttaaa
     1261 tacgactttt gagaatctga caatgataaa tccgaatatt atgaattttg aagatgaaag
     1321 catcattgaa gcttctgggt tcagaatcca accagaatac gaactattca agcgtctctt
     1381 tgatatcatc gtttcgctta ttttactgat cctcgcctca ccttttatgc taatcacagc
     1441 gattttggta aaaacaactt cacctggacc aatcatctat aaacaagtac gtattacgaa
     1501 aaatcaaaag gaattttcaa tctataaatt ccgttccatg agtgcgacag ctgaagcaaa
     1561 atcaggacca gtcttggcta aaagtaacga tgcacgtgtg acaccagtag gtaaatttat
     1621 ccgtgctgta cgttttgacg aattacctca aatctttaat gtattgaaag gcgatatgtc
     1681 gattgtcggt ccaaggccag aaagaccatt tttcgttgat cagttcaatg aagaaaatcc
     1741 ttattattac ttaagacaca atgtacgtgc gggaattacc ggatatgcac aagtatacgg
     1801 aaaatatgta tcagattata acagtaaact acgttttgat ttgttgtata tcaaaaagta
     1861 ttccttacta ttagatttga agattttact tcaaacaatc aaaatcttat ttgataagat
     1921 gtcctcacaa ggactagatg aagatgaaaa tatcagttca ttagcagaaa gtgcatttcc
     1981 aaaagaaaaa acgtatcgtt agattggaaa aattttgagc aaagaagtag gagagaacat
     2041 tggaaaataa aaatataagt atactgcttt ctttgtatga taaagaaaag ccggaatatt
     2101 taaaggaagc attagctagt atctttgagc aaacggtgat gccggcagaa atcgtattgg
     2161 tttatgatgg ccctataaat gagaagcttc aggcagttgt cacttatttc cagcaaaaag
     2221 ttcctcactt gtttacagtt gtaaaattag aaaaaaatca aggattgggg attgctttag
     2281 caactggatt agaacatact aaaaatcgct tagtggccag aatggataca gatgacatca
     2341 tggaaccaca cagattggaa aaacaattag agatcttcca agaatatcca gaaatagcga
     2401 ttgttgggag taatattgaa gaatttactg gcacacttag caatgttatc ggaagaagga
     2461 ttgtacctga gtataatgat gaaatccgtg tgttttcgaa aagaagaaac ccttttaatc
     2521 atatgacagt catgtttgat aaaaatgcta ttttagaagt gggaaactat cgcccattaa
     2581 ttggatttga agattattat ttgtgggtaa gactattgaa ggcgggatat aaaggatata
     2641 atatccaaga gattctagta catgctagag caggtgctga catgtatgct agacgtggag
     2701 gaagtaaata tcttttacca ggaattaaag gccgatatcg cgtatggaaa gatggtctag
     2761 gatcactaaa agattttctg atagttattt gcggacatct tgtaattagt ttattaccga
     2821 ataaaattag gggaaaagta tatgaatcaa aacttagaaa aacaaactaa actttctata
     2881 atcatacctt tttacaatgc aaagaatact ttaagaagag ctgtagacag cgtcttaacg
     2941 caaggttatc ccaatattga aatcttgtta attgatgatg gatctacaga tggtagtagt
     3001 gacattggtg acgaaattgc taatctagag agtaaagtag catgttatca cttggaaaac
     3061 gctggtccat cagttgctag gaactatggg ataaaagaag caactggaaa tttggtagga
     3121 tttttagata gtgacgatta ttttttacct gattgtttta ataatgtttt gaaagactac
     3181 cataggaatc accccgatat cattgctttt ggattaagta aaggaaaaaa tatagaaaat
     3241 gcaaatttat ttgttcccga aaagaagatt ggttgttcaa gtgaagaagc aattgagcaa
     3301 atgtttagaa gtaaggcagt ggacttttac gtatggaata aattttttaa aagggaatta
     3361 ttcaatcata tagtttttcc agaaaataaa ttatatgagg atatggttcc tatgtatgaa
     3421 gcttttaggg cggctagtaa aattgatatt ttgccatttt caggtatttt ttattatcaa
     3481 aataacgata gcatcgttta tcagtctttt aattcaaaac agtatgataa tattttgcag
     3541 agaaaaatat tagttgatga agtaaagaaa gattatcctc gacttattaa tttggccaat
     3601 gctcggttag tagacggata tctttcaaca gggtttaaaa ttactggcaa aaatcaaaat
     3661 cagaaagaaa aacaaaaata ttataatc
//
LOCUS       DAGCWU010000191         3684 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.196, whole genome shotgun sequence.
ACCESSION   DAGCWU010000191 DAGCWU010000000
VERSION     DAGCWU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.196"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            45..497
                     /locus_tag="MBZ71_002556"
     CDS             45..497
                     /locus_tag="MBZ71_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430454.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            511..1200
                     /locus_tag="MBZ71_002557"
     CDS             511..1200
                     /locus_tag="MBZ71_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430455.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1325..3676
                     /locus_tag="MBZ71_002558"
     CDS             1325..3676
                     /locus_tag="MBZ71_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBT4430456.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1255 a          477 c          771 g         1181 t
ORIGIN      
        1 gtagatatat attataaatt taataagaaa gcgaggtgag aatcgtgaaa aaaacgatac
       61 taagtatttt ttcagtattt acaatattaa ttgggggcta ttttcttttg aatttagatt
      121 ctgttaattc aaacgctcaa ataaaagatc aaagagtagc tgaacataat aataaaaaaa
      181 tgagtttgac caaaaatatc tattctatgt cagatgaaga attgtataaa gaatttggtg
      241 atttatccgg ggaacagtta gaagaaagta ttccaggaat tagtgactca gataaaattg
      301 ttgtcttgtc tgctggtggg caattaatgg gagatggcaa atccgaatta gttagttata
      361 aacaaaatca cagacagttt attgatgtta atactgattc caacgctatt tcgattaagg
      421 atctaaaaga gcttttaaat agtaaaagaa atgaattaaa gcagataaaa gaaactaatt
      481 cagtggaatt agagtaaagg aagattcatt atgaaaaaga tttttggtgt ttctgttggg
      541 ctacttatga tgttaacatt tggaaatgca gtatatgctg ctgagaaaga agttatttca
      601 gatccagagc agtacgcctc acgatacttt gctgattttg aagaagaaga taaagtactt
      661 gcattgccag aaggtggttt tttaaaaggg gaagctaaaa ttgtatctat ggataatcca
      721 gatataattc aaggttcata taattcagaa actgatcctg cttcagtttc agtgacagaa
      781 gcaaaaaaaa ttgttgctga aaaattcaaa aatgatattg aaactactcc aattatgcct
      841 cgaggagcat ttccacccga tgttggtact agcggacacc ttcttgtact agcaggagga
      901 ggaagctata aatcacagcc atttagtggt gcgggatggc gttttggagg tatgggtgta
      961 aaaccagctg atggtacagg caacttttta cgttggaaat cctacattga cgatggaaga
     1021 gtaggaaatg taaatcaagc caacgcaact aaatctggtt ctattcaagg aatggcattg
     1081 tatgctcgtg aaggattcaa atactttgca ccaaacgggc ctggttggac aatgaatata
     1141 ggtatttatt acacttataa tcctgttgca ggtacgtatt atgttgttca aaatatttaa
     1201 taaaacagca caaagagatt tctttgtgct gttttattat ttttagttgt tgacaaacac
     1261 taaaatataa aatataatca acacataaac gaacgtatgt tcggcaagaa agcaggggat
     1321 ttttatggat gagatggttt taagtactca gaaatggcta aacaagaaat acagtaatgt
     1381 tacagggttt gataaggttc cagaaaatgg gcgaacaggg tggcctacaa tctatggact
     1441 tattgaagga ctacaagtag aacttggtat tactaatcta gtagctaatt ttggacctac
     1501 cactgaaaaa atgtacgata atcaggttac tcctaaatgg ggcaaaaatc tacctaaaaa
     1561 tattgtattt cttattcaag gtgccttttg gtgtaagggg attaatccag gtggcttcga
     1621 tggggtatat actccatatt tagatactgc tgtaaaagag ttgcagactg atgcagggtt
     1681 taatggaagc gctgtaactg gagttttgga tgcgaaatgg gccaaagcgt tatttgatat
     1741 gagtgctttt gttcttgttc ccggcggaga tagtaaaatt agaaaaatgc agcaatggtt
     1801 aaatgtaaat tatttagaat acacgggaat aatgccttgt gatggtattt atcaaagaaa
     1861 ttcaaatcaa gcattaattt ttgcattaca agctgaacta ggatattctc ccagtgaagc
     1921 aacagggtct tatggtaatg ggacaactag taaaacttat cctgtgtctg aaggtaactc
     1981 gggaaactat gtaagaataa ttcaatatgg attatatgtt aatggttatt ttgaagacgg
     2041 tacattcgat ggtattttca ctaaatatat gggtttagaa gtactggcat tccgaaaatt
     2101 tatggtattg cctgaatata ctgaaatagc agatgttgtt gtaataaaag gactattgtc
     2161 tagcgctgga gatattagaa gatctgcaga tggcgcagat acttctacgc aattaactag
     2221 atctcaaatc caaacattgg ttgataatga tataaaaata gttggtagat atcttactgg
     2281 tactgttgga attggaaaag atgaacgaaa taagtatcta actacagagg aattgaataa
     2341 tattttttct aaaaatttat ctgtgtttcc tatctatcaa gatggaggtg cagcattagc
     2401 ttattttact tatgatcgtg gcttatcaga tggtaagaag gctatagatg cagcaaaaaa
     2461 tctaaatata ccattaacaa cagttattta ttttgctgtt gatctagaca tgttagggga
     2521 agaaattctt gcatatgctg gagaatattt taagggggta tctgctgtaa tgtcatatag
     2581 cgggtatcaa acgggtgttt acggaacaag aaatgtttgt tctcaaataa tcaataacgg
     2641 gtatgcttcg tttgcatttg tttcagatat gtcgactggt tatagtggga atttaggttt
     2701 tcctatgcct agacaatggt cttttgatca atttgttgaa tttaccatag gaagtggcaa
     2761 cggagctgtt ggtatcgatt taatcgctac atcgggtaga gatagcggat ttaatgagtt
     2821 atcaaatgat actaataatg ataattacat tgccaaatac aatcagaagg ttataaatca
     2881 gatggtacgt gcgtatcaat atctatcaca agctggtctt gataatcctt ggaaccctca
     2941 tttgacattt tatagatatg taaattattc tggtttgtca tgggacatca tttcttctcc
     3001 tgtaacagag catgatagaa aaatttatga tgaatatagg aataaattaa ctaatagcga
     3061 gggcctgtat aattatttta tagatcctaa tactggttca ataataggtt tgcctcactt
     3121 aattgtgacg ttacaatcac agatgtttct agccgacaca atcaatgata gtgtttctga
     3181 ctttgcaggt tggttaggag atttaatgac ttgctggggt gaagttaaga aattaggaat
     3241 acaaatggag caaggtgtat ttgcattagt aggtacggct gcaggtggaa catttagtct
     3301 agaagattta taccaagatg ttgacgcggt aaatgtttat accttagcaa aaagaaattc
     3361 tgattcaaca gctttatcgg ctttaaaaac gtactattgt gataagggtg ctattgatag
     3421 atttaatcaa tttgttaaga acagatttgg ttcttcagat aaaatcgctt cagtaacaaa
     3481 tagtttatta gaaaatgacg aaacagttga tatcttaggt gtagggataa tttcattttt
     3541 cattgataaa atagttaata attcacttgg gacagatttt acttatcttg attttattgg
     3601 agatgagggg aaagttgcaa atggattttc tgaaaaaatt attcaatttc aagaaaatag
     3661 aagtaaactt aattaagaat cgtt
//
LOCUS       DAGCWU010000192         3545 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.197, whole genome shotgun sequence.
ACCESSION   DAGCWU010000192 DAGCWU010000000
VERSION     DAGCWU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.197"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..182)
                     /locus_tag="MBZ71_002559"
     CDS             complement(<1..182)
                     /locus_tag="MBZ71_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4430457.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="MBZ71_002560"
     CDS             517..951
                     /locus_tag="MBZ71_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4430458.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="MBZ71_002561"
     CDS             974..1294
                     /locus_tag="MBZ71_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430459.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="MBZ71_002562"
     CDS             1435..1626
                     /locus_tag="MBZ71_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430460.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="MBZ71_002563"
     CDS             complement(1697..1954)
                     /locus_tag="MBZ71_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBT4430461.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="MBZ71_002564"
     CDS             2112..2375
                     /locus_tag="MBZ71_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430462.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="MBZ71_002565"
     CDS             2399..2587
                     /locus_tag="MBZ71_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430463.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="MBZ71_002566"
     CDS             complement(2670..2855)
                     /locus_tag="MBZ71_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430464.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="MBZ71_002567"
     CDS             3070..3441
                     /locus_tag="MBZ71_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4430465.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1331 a          507 c          704 g         1003 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaag
//
LOCUS       DAGCWU010000193         3447 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.198, whole genome shotgun sequence.
ACCESSION   DAGCWU010000193 DAGCWU010000000
VERSION     DAGCWU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.198"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..2232)
                     /locus_tag="MBZ71_002568"
     CDS             complement(<1..2232)
                     /locus_tag="MBZ71_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430466.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="MBZ71_002569"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="MBZ71_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          963 a          762 c          548 g         1174 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaa
//
LOCUS       DAGCWU010000194         3357 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.199, whole genome shotgun sequence.
ACCESSION   DAGCWU010000194 DAGCWU010000000
VERSION     DAGCWU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.199"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..641
                     /locus_tag="MBZ71_002570"
     CDS             <1..641
                     /locus_tag="MBZ71_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBT4430467.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(727..1821)
                     /locus_tag="MBZ71_002571"
     CDS             complement(727..1821)
                     /locus_tag="MBZ71_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBT4430468.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            2010..2771
                     /locus_tag="MBZ71_002572"
     CDS             2010..2771
                     /locus_tag="MBZ71_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4430469.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2886..3326)
                     /gene="fabZ"
                     /locus_tag="MBZ71_002573"
     CDS             complement(2886..3326)
                     /gene="fabZ"
                     /locus_tag="MBZ71_002573"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBT4430470.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         1080 a          667 c          570 g         1040 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata acctataaaa
      661 aaacaaaata aaaaagcaga aataactggc tatttctagc ggattatttc tgcaataact
      721 aattatctat tttttatttt caattaattt aaagcttgac catggtttca aaaagtcaag
      781 gaggaataat tcgtcatcgg agattcttcc tacaacattg acacgtccgt cgtttttcat
      841 atccttcaag gcgatttgtg tttctccttt gtattgaccg tacccttcat tgtcaatcag
      901 tacatcccca tgatgcatat ctcgcgtatt atgaggcggg aaatctttat ctttgtagta
      961 tacacgagtc atcgttgaac gaaggatata ttctgagcga tcccctcgat aactatgaag
     1021 gttatcaaac aagcagattc gttcgttttc tgtaatacct tcttccgtat ctactttcaa
     1081 tgttggataa tccgcgttaa atgcttctgc catttctttc aattcttctt cacttgcgta
     1141 cgcatttcca atagaaatat catcaatcaa tcctgtaagg attaaatgct tcacttgtgt
     1201 cgcaatttca agatctcggt ggtcttccag cgtgcacaag ccatcttgcg taggccaagg
     1261 gccaaactca gcagattgcg aattgacgaa tgccatcgta ttcaaattat attttctgaa
     1321 tttttctgaa caaaaaacaa agtgttcgta tcctaaacca gagtatcgat gaggataaaa
     1381 attatgagaa cctaataaat tatccgtgtt tggcgaataa ctcatgatgt tgtcaacgta
     1441 attcgtgccc gaactcatat tgatctcgat tttgatacca aaaggattgc gcgtcatttt
     1501 tgcttcttct gctcctgtga atccgatatc taagcggacc ccgtaggcac ccatcttatg
     1561 gaaaaaagat aagtcatcgt aagaaatctc caactgttca aataacgctg gattgatatc
     1621 aaccattact tccatgccta ggctattcgc gtaatcaact acttctttaa attctgaaag
     1681 aactttttct ttatcatcat tgatctgcag caaactggta aaaacccgtt tatacccata
     1741 cttatgagct aaatccaagt acgctttgtc cttttcaaat gttgaccgtt ctggataaat
     1801 cgagattcct aattttccca tattaccatt tcctcctgca tcttttcgtt tttgtatcca
     1861 cttacattat agcaaacaca aagtaaatgg tctacacctt ttctttttct tttaaaataa
     1921 atgataaatt ccaataattt aagtaggtat tttccctcta ttttgttatc attaaagata
     1981 acaaaatagt aaattggaaa ggaacagtta tgaagaaaaa taaagttatt ttaagtatta
     2041 gtttagcagc agcattgctg tttgctgctg gttgttctgc aaacaacacg acttcttcta
     2101 gttcgtctac agaaagttca aaaagtacat ctactgcgga aagcacgact gccagtagcg
     2161 tcgatttgaa cgcattggaa ttaccacaat tgacgaaaga tgtcgccgaa aatgaagatc
     2221 tggtcgaaat ggtgacgaat aaaggcacaa tcaaaatcaa attatttccc gaacacgcac
     2281 caaaagcggt tgaaaacttt atgacacatg caaaagacgg ttactacaac ggactgactt
     2341 tccatcgcgt catcaaagat ttcatgatcc aaggtggcga tcctaaaggc gatggcactg
     2401 gaggggaaag catttgggga gaaggatttg aaacagagat caacaaccac ctttataata
     2461 taagaggtgc gttatctatg gctcgctcac aagatcctaa cagtaacggc agccagttct
     2521 ttatcgttca aaatacagat gatatgcatg atggtctttt aaaagacgac tatcctcaag
     2581 caatcatcga cgcttataaa aacggcggtt atccttcact agatggccat tatactgtct
     2641 tcggccaagt gattgaagga atggatgtcg tggatgctat tgcaaatatc gaaacagata
     2701 gttcagacaa accaaaagaa gaagtaaaaa tcgataaaat caatatactg caagaagcaa
     2761 aacaaaaata aaaaaataac gtctgaatcc tgcatagatg acaacgtatg ctaggattca
     2821 ggcgtcttct ttttacataa aaagcaagta agcttccttt tgttttgcta tatcaggttt
     2881 tatagtcaag cattttggtt gacgatgaac gtgaactcgc actcacagac ttttttatca
     2941 tctacatatg ctgcagataa cgctgtaccg actgatccct tcattttggt gatttcaaaa
     3001 tgacatttca gcacatctcc tggaacaact ttttgacgga atttggcctt attgatccca
     3061 ccgatataag ctctttttcc tagaaactct tctgatttta ggataagtat cgaacctgct
     3121 tgtgccagtg tttctagaat caacgcaccc ggcatcactg gattgtcagg aaagtgacct
     3181 tggaaaaaat cttcattgat tgttacattt tttgtcgcaa cgatcttttt tcctggttct
     3241 aaagtatcta cataatcaat ataacaaata ggataacgat taggaatcaa ttccattact
     3301 tcagtagcag ataatacgta agacataatt aaactccttc tttcaggcaa ttgccat
//
LOCUS       DAGCWU010000195         3302 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.200, whole genome shotgun sequence.
ACCESSION   DAGCWU010000195 DAGCWU010000000
VERSION     DAGCWU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.200"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            93..506
                     /locus_tag="MBZ71_002574"
     CDS             93..506
                     /locus_tag="MBZ71_002574"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4430471.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            663..920
                     /locus_tag="MBZ71_002575"
     CDS             663..920
                     /locus_tag="MBZ71_002575"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4430472.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            1007..2245
                     /gene="istA"
                     /locus_tag="MBZ71_002576"
     CDS             1007..2245
                     /gene="istA"
                     /locus_tag="MBZ71_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBT4430473.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            2235..2975
                     /gene="istB"
                     /locus_tag="MBZ71_002577"
     CDS             2235..2975
                     /gene="istB"
                     /locus_tag="MBZ71_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBT4430474.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
BASE COUNT         1061 a          671 c          651 g          919 t
ORIGIN      
        1 ctgtcggtcc tagatttttt gtaaaaaaaa acagacataa aaaaattccg atagaataga
       61 gtcgtctaaa acatcaatct gaaaggaagt ttttgatatc catttctcat tctatcaaag
      121 agctcttcac tattaaagat aaaaatattg aaattaattc ttttcaagaa actttttata
      181 aggacaagaa agccatcgta atcgaaggtc cattagttcg cacttttaga cattgtctat
      241 tttgtaaaga atctgcaaag cagatcgaga aaaacggcaa gaagatttca atgattatct
      301 taaatcgcag tgggaataaa caaacctatt tgcgtcttag aaaacaacat taccactgcc
      361 aatcatgcgg gaaatatttt actgctcata cgtatcttgt cgctccttca tgttttattt
      421 cgaaacaagt tcattacacg atattagaag agttgaccga gcgtcaagca ttaaagacaa
      481 ttgcgaaacg ttgtgatttt cagtaacgac agttcaacgg acattatctg ctctatcggc
      541 aacgctgcga cagaagctag actatttacc tcattgttta ctattcgacg agtttcgctc
      601 tctttcaatt ttccatggga agttctcctt ttcttgtatg aattgagaga cagggaagct
      661 ctttgacatt ttgtccagcc ggagaaaaaa aagatttgat tgcttatttc atgcgtttta
      721 caagaaaagc tcatatgggg gttcgataca ttgtcacaga tatgaatgcc ccttattttt
      781 cattagtcaa agaacccttc ccaaatgcac aaatcatcat tgattgcttt cacattgggc
      841 aacacttgaa caagtttttc gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa
      901 aatgtcaacg gcacctttaa aatgtaggaa aacggcaatt taaaattgtt ggtttaccta
      961 catttttgta tgctcttctt atcccaaatg aaatggaggc tcagggatga gaaaagacat
     1021 acgcgaagga gtgatgatct acgtgatcaa tgaaatcaaa cccaattatg ctgcactagc
     1081 aaaacaatac gactgcgact accgaacagt taagcatgct tacgaagaag cccaagttaa
     1141 agaatcaaaa cctccagaac gtaagaagcg tccaagtaag ttagatccct acagagaaat
     1201 catccaagac aaaatcaaag accaatgcag tgcttacagc attttcaagt ttattgagca
     1261 caaaggattt ggaggaagct acagtttagt aaagacctat tgtcgccaga ttaagcaggc
     1321 gaaagtacac aaggcaaccg tccgagtcga acatagccct gggctttctg ctcaggttga
     1381 ctggaaggaa gatatgcgac taatcagtaa tcagggcgaa atttttgagt tcaatatctt
     1441 tttgtacgtc ttgccatatt ccaagaaaaa atatgtcaca ctaactttcg accgaaaaca
     1501 agacacactc tttgagtgct tagatgatgc gttttatcaa accggtggcg ttcccaaaga
     1561 aatctggttc gataatatga agacagtcgt tgaccattct cgtagtcagt tttccaaacc
     1621 gcatttcaac aaacgttttt atgctttcag taaggatgcc ggcttcacac caattgcctg
     1681 tcggccattt cgtccgcaaa caaagggctg tgtcgaagct ttagccagaa ctgttgaccg
     1741 cctacgggtc tataatcatg aattttatga tgccgttgag ctgtgtcatt tagtgaataa
     1801 cctttgtgtt gagttaaata acgaggtttc tcaatcaact gatcaaacac cagatgaacg
     1861 ttggcagacg aacgaaaaag agcacctgca tccataccaa aaaaaccttt tgaatcccta
     1921 ttttgaagac tttattcgcc gtaaagtcac caaggaagaa atggtcattt tccgtaaatg
     1981 caggtactct gtggcgcctc gctatatcgg taaagaagtc gaaatcgaac tctccgataa
     2041 agaagaccaa gtacaaatct attataacgg agaactgatt cggtcgcatc cattaacgac
     2101 aaaacagctg aactaccacg aggaagacta tttccaaatc ttgaaatccg atgtcatgag
     2161 tcacaaagaa gacgatggaa tcagagcgta tactcgtgaa agtctcaagc aatatgatct
     2221 ggtggaggtt gccgatgaag aatagccatt accaagaact gatgaccaac ttagagactc
     2281 ttggtttgaa tcatatgcgg gaatatgttc ctaactacat cgaagtggcc aatcgagagg
     2341 agctttcttt aacggaagct cttctcgagc tgacacgcag agaagttgat catcaaaata
     2401 agcaaaaaat gaatcgtgtg gtggcacgcg cccgcttttc caagagaacc agcctggaag
     2461 agtttgattt tgccttccag ccaagtatca ataagcgcga agtgctagac ttaaggcaca
     2521 tgggctttgt ggaaaaacat gagaacttgg tttttatcgg cagtcctggt gtaggcaaga
     2581 cacatctgac gattgcattg ggaatcgaag cctgtcgtca aggctatcgg acactattca
     2641 tcacctgtca tgagctgctt ttgcgtctgc gaacagccta tgaaaaaggt accctagagc
     2701 gtgttctcaa acgatacgct cgctatgacg tcttgattat tgatgagatt ggctatcttc
     2761 cgattcaaaa acaggaagct gatttattct ttcagcttct tgccatgcgc tatgaacacc
     2821 gctctacgat cattacgacg aacattgtct tatctcgttg gggggagttg ttccaaagtt
     2881 ctgagatcgc agctgccatc cttgaccgtt tagtacatca cgtcaaggtg ttcaagatca
     2941 caggcaagtc ttatcgtatg agagacaaga tctaaagtgt cgattgtcga caattactat
     3001 tgccgaaaac ctacattttt atcctgccca aaacctacaa ttttaagctg cccttgacat
     3061 caaaaagaca cttcacaagc taagtgctat cgacaattga aacctttgta taaattactt
     3121 ctataatcag atgacgagtt ggattataca acattcaaga aatggcagaa ttttcaatgg
     3181 cgctatttaa cagaaagtga agtagttgat tgtctaccct caatctctta tgaacttaaa
     3241 atggcttagc agtattatca aaagctgctt aacaattatc acaagaaaca gctagctact
     3301 tc
//
LOCUS       DAGCWU010000196         3263 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.201, whole genome shotgun sequence.
ACCESSION   DAGCWU010000196 DAGCWU010000000
VERSION     DAGCWU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.201"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(451..1761)
                     /locus_tag="MBZ71_002578"
     CDS             complement(451..1761)
                     /locus_tag="MBZ71_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBT4430475.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="MBZ71_002579"
     CDS             complement(1773..3191)
                     /locus_tag="MBZ71_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4430476.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1000 a          668 c          506 g         1089 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cac
//
LOCUS       DAGCWU010000197         3193 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.202, whole genome shotgun sequence.
ACCESSION   DAGCWU010000197 DAGCWU010000000
VERSION     DAGCWU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.202"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..924
                     /locus_tag="MBZ71_002580"
     CDS             <1..924
                     /locus_tag="MBZ71_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBT4430477.1"
                     /translation="NSKYSKMKDSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQA
                     DLMDKLNCYQGKVKTILNELETYNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLF
                     IQNSFDDDLSETIDKSTSSRNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHL
                     KDTETDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNA
                     KHKAEELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNW
                     FIEKAIAAKLANEQRETFNELPTVSTENWLE"
     gene            1782..2300
                     /locus_tag="MBZ71_002581"
     CDS             1782..2300
                     /locus_tag="MBZ71_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBT4430478.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1185 a          460 c          573 g          975 t
ORIGIN      
        1 aattctaaat actctaaaat gaaagacagc tcaaaaatag cctatatgct ttttaaaaat
       61 gaatttcgta gagccttaca tgaaaaatgg atcgacgatg atggtctatt atatttggaa
      121 tttacacaag cagacctaat ggacaaatta aattgctacc aaggcaaagt aaaaaccatt
      181 ttaaacgagt tagaaacata taatttgatt tcagtcgtaa aaggagaatt taacccaaaa
      241 acgaaaaaaa atgaaaaaaa taaatactat ttacttcaac ctgaaataag tgccgatgat
      301 ctttttattc aaaattcttt cgatgacgat ttaagcgaaa ctattgataa atcaacctct
      361 tctcggaatg cggaaatcgc acgtcgagaa aaaacagctg aaacccttga taacactgga
      421 aatgcggaaa tcgcacaaga ttattatatt aataaatctt tagacactaa aagacactta
      481 aaagacactg aaacagacca attacaaaat caagttttgc tagataactt cgttgaaatc
      541 atgaaagaag acagcataaa cacatttgta ccagaaaagg tattaagctt gataaaaaca
      601 ttttctaaca cttacgaaga agcccaacaa accgtcacca caatacacaa cgcaaaacat
      661 aaagccgagg aacttgagaa gacggtagtt gtgtatgaag aacttgaatc ttacggaatt
      721 gatgcggata aaggattata tatgacgttg ctgaaagctt atcaaaaaca aaagactgag
      781 aaagtaaata atttgcagaa tttgattttt gtgtatgtaa aaaactggtt tattgaaaaa
      841 gctattgcgg caaagcttgc aaacgaacaa agagaaacat ttaacgaatt accaacagtt
      901 agtacggaaa attggctaga gtagttgata aaaagcgaac actataaaaa tgatcaattt
      961 taaattagaa cttttttaca attttaattt tattacaaat gttacaaata aatagttatt
     1021 attccggagt ttttagatta aaagtttatg aaaacaatca caaacgtagg ttttaatgtc
     1081 ataatcgtta gatattttag ttatttttca ttcaaaaaaa cagggaacta gtgtttgcat
     1141 atgtattttt ttgagttata ataattcatg aagagaggta attcacttat ttacccaagc
     1201 tgaaaagcct gatattactt cttttcaggc agtgctgcta atattcaatt acactttttt
     1261 gtataaattt agttgtttta aatttaattg tctgctatgc ttgtgatgaa ttgattttcg
     1321 ttctgcactt ttggacgata aatttggaca attgaaaata caaaaaaaaa gaaaggccct
     1381 gtgagactcg gaaggctacc aaccttcgtc gacgtccaca ggtggcatta agatcactcc
     1441 cttaatgcgt ttacctttca gtgttacggt attacccgta acggatttag tataacagat
     1501 attcaaaagt gataccactt tttttggctg tgttaaggtt aaaaatacgt gatattacga
     1561 tgaatctatg cctaagcata atgttataaa aatgtccgtg tggctctttg ccgtatgacg
     1621 aacgctgaaa ttaggagacg ttgggatatt ttataaatca ttcacatatg agttagacag
     1681 tcaatgcata tcagatatgc attgtagaac ggattaaaat atcgtggatg aaattataga
     1741 tgaattaaag gagacaatta ggaatttatg gaggtaatat aatgagaaca tataaaaata
     1801 aagaagaatt aaaaaaagaa ataaatagga cttatactaa atatatttta gaatttgaaa
     1861 ctattcctga agaattgaag gataaaagag ttgaggaagt ggatagaaca cctgctgaaa
     1921 atttagcata tcaagttggt tggacgaatt taattctaaa atgggaaaat gatgaaagaa
     1981 atggattatc tgttaaaacg ccttctgatc aatttaaatg gaatcagtta ggagagttat
     2041 ataagtggtt tacaaatacc tatgcacatc taccattgaa agaattagag gaaattctca
     2101 atcgtaatat tgatgatatt aacatgatga ttgattcaat gagtgatgaa gatctattca
     2161 ctgcacacaa aagaaaatgg gcagacgagg caaccaaaac ggctgtatgg gaagtatata
     2221 aatttattca tgtaaataca gttgctccat ttgggacttt tagaacaaag ataagaaaat
     2281 ggaagagatt agctttgtaa attcccgttt actgctaagc aattgtggaa aggagacctc
     2341 ttgtatgtta acagtaagaa catacgacta cataagttct ttgctaactg ccggttttta
     2401 ttgggtgaat gcaagtgcac tcccctagaa tgccgtagag ggtgcatcgt tagattgtat
     2461 caattttggt tggccaacag ataaaccatg actcacttcg ataaaatgag attatggtgc
     2521 catatccgct acaaagggac ttaggagact agcaggggaa agattttttt atttttggga
     2581 tgtttttcag accgccgata gtaccgacaa ttccctttaa atatggaatc tcaacaaccc
     2641 acatgaaggg taaggcaagt ccggtaatta tatcttctga ctttgggtta ggaagaattg
     2701 aaattgttaa gaacgattca acgcttatgg tttataattt ggagatagcc tttgttaata
     2761 tgctaaagcc aacatacgat acagattttg agcagtttat tctaaccatc aaagggtatg
     2821 tggcctatcg cctatcctat tactgatact gaagagatct taaataaatt ctatacggga
     2881 tttgaagatc ctgaaggtga aatcgtcatt tatcagaata aataaatcaa atagtagaag
     2941 ttcaaagaat gaggggaaca ttagttttta ttgcttagtt taatcccagt accgatttct
     3001 gcaagatgta aaaggtcttt catttcataa gttttcagaa gcaagttcat acgcactaac
     3061 tcactttcga ttgagtagca atcgtcaaca aactttacta tcagctgtta tttttattct
     3121 ttcgtgttat ctaaatacca tcaatttata agttgtaggt gtatttaata aaagaacaaa
     3181 taactttagt att
//
LOCUS       DAGCWU010000198         3112 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.203, whole genome shotgun sequence.
ACCESSION   DAGCWU010000198 DAGCWU010000000
VERSION     DAGCWU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.203"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            38..1777
                     /gene="yfcC"
                     /locus_tag="MBZ71_002582"
     CDS             38..1777
                     /gene="yfcC"
                     /locus_tag="MBZ71_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBT4430479.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1934..>3112
                     /gene="iadA"
                     /locus_tag="MBZ71_002583"
     CDS             1934..>3112
                     /gene="iadA"
                     /locus_tag="MBZ71_002583"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBT4430480.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     S"
BASE COUNT          951 a          530 c          742 g          889 t
ORIGIN      
        1 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
       61 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      121 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      181 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      241 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      301 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      361 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      421 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      481 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      541 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      601 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      661 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      721 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      781 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      841 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
      901 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
      961 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1021 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1081 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1141 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1201 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1261 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1321 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1381 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1441 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1501 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1561 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1621 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1681 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1741 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1801 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1861 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     1921 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     1981 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2041 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2101 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2161 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2221 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2281 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2341 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2401 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2461 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2521 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2581 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2641 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2701 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2761 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2821 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     2881 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     2941 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3001 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3061 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gt
//
LOCUS       DAGCWU010000199         3110 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.204, whole genome shotgun sequence.
ACCESSION   DAGCWU010000199 DAGCWU010000000
VERSION     DAGCWU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.204"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(37..1332)
                     /gene="purB"
                     /locus_tag="MBZ71_002584"
     CDS             complement(37..1332)
                     /gene="purB"
                     /locus_tag="MBZ71_002584"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBT4430481.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1345..2469)
                     /gene="purK"
                     /locus_tag="MBZ71_002585"
     CDS             complement(1345..2469)
                     /gene="purK"
                     /locus_tag="MBZ71_002585"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBT4430482.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2462..2950)
                     /gene="purE"
                     /locus_tag="MBZ71_002586"
     CDS             complement(2462..2950)
                     /gene="purE"
                     /locus_tag="MBZ71_002586"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBT4430483.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
BASE COUNT          831 a          733 c          549 g          997 t
ORIGIN      
        1 attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta ctcgtttgaa
       61 tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta tttcttcttt
      121 agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact gcacttggtt
      181 gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc ttgtcattcc
      241 cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg ttgcagacat
      301 attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc caaaacgatt
      361 taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg ctgaagaatg
      421 agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca tgtgtccgcg
      481 caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt tgtgcggcat
      541 agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg tttcggattt
      601 ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa gcgccatcgc
      661 tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt cttgtggacg
      721 gatccccaat tgtttacata catactcttc aataaatggc gggatatttg cgaacgttcc
      781 cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa atcgttcgat
      841 gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag ttggttcagc
      901 atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg ctttttcgcc
      961 aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt gcttgatgag
     1021 atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga cccattttct
     1081 ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat ggcgtgttaa
     1141 tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa tttttttcac
     1201 atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa tctcgacttc
     1261 gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag ggcgtgtata
     1321 acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga tttcttccag
     1381 cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac ctttttttgc
     1441 ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca agcgatagct
     1501 ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca aacgtacttt
     1561 ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac tcatagaaca
     1621 agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat tcacatatag
     1681 cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc tcactgcttc
     1741 tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa tcgcggctgg
     1801 agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg gaaaagtcgt
     1861 gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa acgggatcca
     1921 tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta gatccgcccg
     1981 acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt tcaaaacaca
     2041 aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga tcgttgcata
     2101 aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc ggtcttgcgt
     2161 aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg catttaacgc
     2221 ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg caagctcttt
     2281 cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt gtgcggctgg
     2341 acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg cactaatcgc
     2401 catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag gcaacaaagg
     2461 tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa cgtctttcag
     2521 ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca agtagtccag
     2581 cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct ccaggcattt
     2641 ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc ggtacaccga
     2701 taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct ccccctgctc
     2761 ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca aacatcaaat
     2821 ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat tcatccaata
     2881 tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc acagagattt
     2941 ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa tcactttttt
     3001 tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc gtgtttatta
     3061 aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
//
LOCUS       DAGCWU010000200         3104 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.205, whole genome shotgun sequence.
ACCESSION   DAGCWU010000200 DAGCWU010000000
VERSION     DAGCWU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.205"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(132..1076)
                     /locus_tag="MBZ71_002587"
     CDS             complement(132..1076)
                     /locus_tag="MBZ71_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBT4430484.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1210..2601)
                     /locus_tag="MBZ71_002588"
     CDS             complement(1210..2601)
                     /locus_tag="MBZ71_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBT4430485.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHNKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2727..>3104
                     /gene="tagD"
                     /locus_tag="MBZ71_002589"
     CDS             2727..>3104
                     /gene="tagD"
                     /locus_tag="MBZ71_002589"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HBT4430486.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIK"
BASE COUNT         1105 a          542 c          434 g         1023 t
ORIGIN      
        1 tttcatgtaa aatatttaat tatatatttc ctaaataaaa agtaaaacaa gaattaaata
       61 gttggttata caactttaat tcttgtttta ctttttagaa ttatttatcc aaagatgcaa
      121 tcattttttc atcaaatttc tgtctttaaa taccgttcca ttccgctcaa tatatcaaat
      181 ttaggctgat agcctaaaga acgaattttt gaaatatccg ctaaggaatc acgaatatcc
      241 cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg atcaattgaa
      301 tggatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg ctgtcctaaa
      361 gctttttctt ccttggcaac aagtaaaagt gcttgtataa catcttctat ataaacaaag
      421 tctctagact gactcccatc gccaaataaa gtaaattccg ctttttctcc tgccaattgt
      481 tttttgtaac gatccactag aatcgaaatg actccgctat atggagagtt tggattttga
      541 tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata taaatgacag
      601 taatccaaaa catattgttc tgctgcaaat ttatcgatag catatggtgt taatggacgg
      661 atgactgatt cttctttttt tggcagtgtt ggttcatcac cataaactgc agctgatgaa
      721 ctaaatacta atcgttttaa attagattga tatttccgtg ctaactcaag caataaaagc
      781 gcactgtcaa aatttacacg atgtgtttct acaggccgtt ctacagaatc tgccacacta
      841 gctacagcag ctagatgaaa aatataggca aactgatatt ctttcatgat tctttccatc
      901 aattgagaat ctgatacatc cccctcaatg aaagtcactt gtttatccat atccaagttt
      961 tctttttttc ccattgataa atcgtcaact actacgattt tgttttcttt tcccaaataa
     1021 ttcgctagcg ttgaaccgat aaaaccagct ccgccagtaa tcaatatagt gtccatcaaa
     1081 ttcactccaa ttgcacattt tcgtatcaaa taatattagt ttaccatttt tatattgtaa
     1141 aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt catataccta
     1201 aaaatagttt caattagtta ctccttcttc tttagatata gttttttgga tagaacccag
     1261 atatccccaa aaaataaatg aacctatcgt actaaccaaa atgagttctg tgataaataa
     1321 tgccgaaact gctattgtaa gaatcattaa aatagcaaaa aagtgcttat cttttactag
     1381 atttggttgt gtgaaacaat aacgcaaggc tttaaagcat tggataaata agaaggttaa
     1441 caatgtcaat agacctaatc ctcctgtagc agccatcact aagaaaataa aattatgagc
     1501 agttaaagta tcttttccca ttaatgtata tggtagttta ttatgtgcat attcaatata
     1561 gtagcctgga gaacctccgg atatcggctt actcttgaat atttcaattg cacttttcca
     1621 aagttcaaac cttaaattag aaacatcccc gctatcagtg tcagggcgtt ttaaatcaac
     1681 tttattgctg ctagttgttc ctgatgaatc agatgtatct ttttttactt ctgtaatatg
     1741 cggaacatgt gcccatgcat gagcagttat atctgttaca taatacatcg caattatact
     1801 tagcaatcct actactaatg cagttagcca tctttttaaa gtattagttt taagaaaacg
     1861 actactataa cttgtaaaga aactaaaaac aaaagttgtt attaataaac aaactaatgt
     1921 ggtgcgtgaa cccgataaag caataaacga gaattgaata aaaatattaa ttacaaaaaa
     1981 tattttaaat agaagattta aatgcttgta agtgaccaaa taatatatag caataatgat
     2041 gacgattaat gatataaccg caccaaaatt tggatctgta tatacaccaa ataaacggtt
     2101 gcctaaccac cctaaacgaa ctgggtaata cttatatttt attggaattt ttatgccata
     2161 ttgaatgaca aaccagtata atgatactaa attagctaaa aaccatacca atgaaagtat
     2221 ccaggtaaat actttgaaaa aaaagttact actatagtct ttgccaaact gataaatcat
     2281 aaagaaaaag attaattgcc acatgattaa cttaatattt tcgctatatc catattttaa
     2341 attcattaaa ctgcttacta gcatcgcgcc aataaaagca attaatagcc aatcatattt
     2401 ttttagattt ttttctctaa atgcaataat tgcatctata atgatcaaga caactcccgc
     2461 tattccagca ggccaaaaaa gtaaagtatc atatctagta ggtaatagta aatacgggcc
     2521 tattgctcgt aacattaaaa taaaaccaaa ataaataaaa taccaacctt tcacatattt
     2581 taaaaatctt tcattcaaca tttttcctcc atccacctat ttctcataaa tatttcttat
     2641 cctttttagg ataaactggt cctttcaatt ctaacaaaaa aatgctaaac taacaatgta
     2701 tattaatgaa tttcgagagg tgaaagatga aaagagtaat tacttacggt acctttgatt
     2761 tgcttcatta cgggcatatc aatttactga aacgagcaaa acaatacgga gactatctta
     2821 ttgttgccct ttctacagat gaattcaatt ggaatgcgaa acaaaaaaaa tgttatttta
     2881 gttatgaaaa aagaaaacag ttacttgaag ctattcgcta cgttgattta gttattcctg
     2941 aagaaaattg ggaacaaaaa gtaaaagata taaaagaata ccatgcagat tatttcgtga
     3001 tgggcgatga ttgggctgga cattttgatg aacttgaaca actaacagat gcacaagtga
     3061 tttatttacc aagaacacct gagatctcta caactcaaat aaag
//
LOCUS       DAGCWU010000201         3093 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.206, whole genome shotgun sequence.
ACCESSION   DAGCWU010000201 DAGCWU010000000
VERSION     DAGCWU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.206"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            63..818
                     /gene="fabI"
                     /locus_tag="MBZ71_002590"
     CDS             63..818
                     /gene="fabI"
                     /locus_tag="MBZ71_002590"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBT4430487.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(928..1941)
                     /locus_tag="MBZ71_002591"
     CDS             complement(928..1941)
                     /locus_tag="MBZ71_002591"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBT4430488.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            2106..3026
                     /locus_tag="MBZ71_002592"
     CDS             2106..3026
                     /locus_tag="MBZ71_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBT4430489.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          899 a          624 c          590 g          980 t
ORIGIN      
        1 agctattatt ctctattttt ttacgactat caaattttta tcagaaacaa aggagagtca
       61 ttatgtcatt tctagaagga aaaaaaatcg tcattatggg cgtcgctaac aaaaaaagca
      121 ttgcttgggg ttgtgctgaa gcaatgttga aacaaggggc agaaattatc tatacttacc
      181 aaaatgaacg aatgaaaaaa tcattagtca aattggtcgg cgaagaagca ttcactgtag
      241 aatgtgatgt ttctagcgac gaaagcatcg aaaaagcaat gaatgccatc ggtgaatatg
      301 ctggggagat ccacggcctt gtccatgcag ttgcttacgc caacaaagaa gaattgtccg
      361 gaaatgtgtc tgatatttcc agagacggct atcaattagc tcaagatatc agcagctact
      421 ctttaattgc tgtatcaaaa tacgcacaac cgtttcttgc accaaacagc gggatcgtta
      481 gcatgactta tctaggttca gaacgcgcaa tcccgaatta taacatgatg ggtattgcga
      541 aagcttctct tgaagctgct atccgttatt tggcagctga gttctcgcct aaaggaattc
      601 gggtcaatgg aatctcagct ggggcaatca aaacattagc tgtcacaggt gttaaagact
      661 atcaaaaact gatccagtta tctgaatctc gtacgcctga tcagcaaggt gtgacgatcg
      721 aagaagtcgg taatgcttgt gctttcttaa tcagcccact ttccagcgga atcatcggtg
      781 atattatcta cgttgacaaa ggtgtccatc tttcataaac ttttgttgca ttctttaaag
      841 taaaacatat aaaaatcagg aaaaaacttt ttaaagtctc ttcctgattt tttcttttta
      901 cgtttttttc aactcattac tttgaaatta ttccacgact tcaatccagc cttctggtgc
      961 ttctatgtct ccaaattgaa tacctgtcaa ttcatcatac aaccgttttg ttactggtcc
     1021 tacttccgtt tcactataaa atacatgaag attgttattg tgataaatcc caccgattgg
     1081 agaaataaca gccgctgtac cacatgcacc agcttctgca aattgatcta gatcatcaat
     1141 gaaaatatct ccttcaattg cttgcagtcc tagtcgttct tcagctaact gcaacaagga
     1201 atatttcgtg atacttggta agatagaggg cgattgcgga gtgatgaatt ttccgtcttt
     1261 tgtaattcca aagaaatttg ctgcacctac ttcttcgatt ttcgtatgtg tggctggatc
     1321 aagataaaca caatctgagt aatccagttc ttttgcttct gttcctggaa gtaaactcgc
     1381 tgcgtaattt ccgccgactt ttgctgcacc agtcccctta ggagcagcac gatcgtaatc
     1441 agatacaata aaattcgttg gttttagtcc acctttgaaa tacgctccta ctggcgtaca
     1501 gaagacgaca aataaatatt ctggagctgg atggacacca atgttttcac cgaccccaat
     1561 cagtaaaggg cgcaagtata atgtagcgcc tgttccatag ggcggcacaa aatcttcatt
     1621 tgcttttacc acttgcttga cagcatcaac gaacatcttt tcagggacca ttggcatcag
     1681 caatcgtcta gcactttgat tcatccgtcg actattttgt tcaggtcgaa agagattgac
     1741 cttgccgtct tttcgacgat aagcttttag accttcaaaa cattgctgac cataatgaag
     1801 cgctggagag ccttcatgaa tcgtcaggta attatcttct gtcaattctc cttcttccca
     1861 ttgtccgtct ttccatttgg caataaaacg ccaaggagtt ttgatataag aaaaacctag
     1921 attgttccaa tcgatttgca tatacttacc accttcgtta ttttctccac attttatcat
     1981 tatccatatt atttgtcatt ttattttcag cttttttcct tcattatagt tatttttgct
     2041 atttcctttt ttgaaaactt cttggtacaa tacagaaaga atcaaatttt agaaaagagg
     2101 attttatgtt ttcattatta gcaactgccg gtacgacaga ctcttctggc gagacattga
     2161 cagatcaagc tgtcagcaaa gtaaatgcgt ttcagcggtt ttgggacggg attgattggg
     2221 acgccatttt ggctgccttt atccaaaaaa gtttatatct gatttttttg atcgtccttt
     2281 tttggattct tcaccgtgtg ggaaaatatc tgattgataa gtcttttaat cagtattcta
     2341 aaaaagccat tctaaacgag agccgcttaa agactttaca tagtctttta aatactattt
     2401 ttcattatac ccttggtttc ttttttatct atgcaatttt atctgctatc ggtgtgccga
     2461 tcggatcact tttggctggt gccggaattg caggtgtggc aatcggtctt ggtgcccaag
     2521 ggttcatgag tgatgttatt accggatttt ttattatcat ggaacaacag atggatgttg
     2581 gggactacat caagctagca aacttaacga ttgaaggaac cgtcgtatct gttggtatcc
     2641 gtacccttca gctgaaagca acggatggta ctgtccattt catcccgaat cgaaacatca
     2701 ccacgatcag caatctctct cgtgcaaata tgcaagtact tttagatatt cggattgttc
     2761 ctgaagaagg atatgacaaa atctatgaga tcattgatcg agtaaatcgt acattagctg
     2821 aaaaatacca agaagagctc caaactgaac cttctatctt tggattagtg gatcttggaa
     2881 acagcaattt tgcgattcgt acggtctgct acgcattgaa cggaaaacaa tatgccttaa
     2941 aagaagagtt cttgagctcc tatgtcaaag aattgacagc ttctggtttc acgattccga
     3001 acagtccaat tgctgtcgga aaataatttg gacaaacgat gcaacgaatc tcactaaaaa
     3061 gagcgtggga caaaagtaaa gaacactttt gtc
//
LOCUS       DAGCWU010000202         3007 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.208, whole genome shotgun sequence.
ACCESSION   DAGCWU010000202 DAGCWU010000000
VERSION     DAGCWU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.208"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(276..692)
                     /locus_tag="MBZ71_002593"
     CDS             complement(276..692)
                     /locus_tag="MBZ71_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430490.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(695..979)
                     /locus_tag="MBZ71_002594"
     CDS             complement(695..979)
                     /locus_tag="MBZ71_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430491.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1679..1774)
                     /locus_tag="MBZ71_002595"
     CDS             complement(1679..1774)
                     /locus_tag="MBZ71_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBT4430492.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2427..>3007
                     /locus_tag="MBZ71_002596"
     CDS             2427..>3007
                     /locus_tag="MBZ71_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4430493.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALREL"
BASE COUNT          957 a          478 c          565 g         1007 t
ORIGIN      
        1 aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat
       61 cgccaaatta gaatttactt tttcaagcct aacgtcagat ttgccaaaga tttctctgcg
      121 taactggtta gaagaataaa taaaaaagaa ctcgcccccc ttacattaag caagtagaca
      181 agtcccttac aaccgactaa gattgctagt acattctagc atattgtatg gggcttctaa
      241 ataaaaaaca gataggaata actcctatct gttttttatc gttttagaaa atttgttggg
      301 tcgaaatttc tagaagtgac tgtttgactg gtttcttttt gatgattctt tggtcgattt
      361 cgatttgatg gatttagttt agcttcgatt ttttctttca cttcttgata tactttagaa
      421 tttgtgccaa taagaacgcc tgtatctttt agatataaat cagcaaattc tgcgatgatt
      481 tcagtcatga tggaaatttc ttttcccata gcatttagtt ttgcctgatt gctggtagtg
      541 gtcgtgttgg tatcgggttc ataactcata cataaaaagc gaatggcttc cgagtagtta
      601 ttgatatctg gatttttttc gatgatggaa tctaaaacgt caacatgact agggtacaaa
      661 gtaacatgtt tttggacaga agtttttctc atggttatat cattcctccg ataagcattt
      721 caatgatttg attatttgtg tagacatact cgcttaatat ttgagtattt tctgtttgga
      781 tagcaataaa tttatcgagc aataaatcga attggtcttt tttgtaagtc gtaagttgac
      841 ggtcaaaatc actttcgatt aaaactttta gatatttatt tcgactccat ttttctcctg
      901 ttttattcgt gagttcttgg caacgtttgt ctattgttgc aacaaacttt ggatcaatat
      961 tccgaactaa aatgtccata aaaaatctcc ttttgcaatt atgtataatt tatgtatacc
     1021 atacaaatac tatatttgta tagctgtttt ttgccaaaat gctatacata agctacccac
     1081 gatggcgaaa aagtgatatc aaattttttt ctgagaaacg tgataggcac agcctatcta
     1141 cccacttttt ttagaaagtg ggtacacgtt tccttatgct ctttaaaatt cgggggggag
     1201 tgctgcacgt gtaatcgata accaaattta acagacgaaa aattgttgga atataaaaat
     1261 atgacacccg aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc
     1321 ggaatttcag acgcagcttt tgacgcatgg agcgaatttc atggagaata taattaagtg
     1381 ggtttaaatg gctctaaaat cgcttctaag agttttttag cttggaccaa tgaaatacac
     1441 caaaaactaa ataaaacgtc ttaaatcgaa aaatacgagc tctcagagcg actttatttg
     1501 taaatgttgc atataagaaa taattacgga ttttagactt ttgtaggata tttttgaaat
     1561 aaatatttat ttctgttata atttgattgc aaataagtga acacgaaaca cacgaaatcc
     1621 ccagagaggt cggattctct ggggcttttt ttgtgggctg agtcgcctta attaagtttt
     1681 acttcttgtt tcggttattc aaccagtaac tgaacaaagc gcttaaaagc ccaacaagaa
     1741 gggagcaaat aaagttagtg aacacgaaac acacctcctt ccagtcgcaa gactgtcgga
     1801 taggcgacaa tcaattatac aataaaaaag caaaaataag cctaagtttt ttattgcctt
     1861 ggggttgtta gggcttgccc tgaccgcctg taaggcgctt tagaacgaag gacatagttt
     1921 ttatcgaatc ttgtcttaga acggcttaga cgagctctca ggacgccaca aaggagtccc
     1981 taatgaatta tttttggcga aggagcctaa aggcgactgc tgcaagtaaa atgtgagagc
     2041 gtagcgatca ctccttctta cagcgaccat agggagctag attggcagtt taatatcatg
     2101 cgcgaacctg tttattttaa aaagtgggtg attttttggc gggaaatttt gctcgcgcgc
     2161 gcgcgtttat tttatttatt ttttaaagat tttttagaac cctttttagg gggcagttga
     2221 gccttactct cccaacggat acagaagggt actaacaact ttttatctga gtactaacaa
     2281 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2341 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2401 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2461 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2521 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2581 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2641 aaaagctcta taaagatatg cttaatctta cttaccgtac tgaagatgag gattctattc
     2701 gctactttgt tttgtttact ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca
     2761 gtgttaatcc ggacctagaa tatattatta atggtttaac agctgaattt agtagatttg
     2821 aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa aactctattc cgactgctta
     2881 tgcagtaccg ttctactggt tattatgtag ttcgtattga agagtttagg agcttaatgg
     2941 atatcccaga atactatcaa atgggtaaca ttgatcaaaa agtattaaat cctgcattac
     3001 gagaact
//
LOCUS       DAGCWU010000203         2972 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.209, whole genome shotgun sequence.
ACCESSION   DAGCWU010000203 DAGCWU010000000
VERSION     DAGCWU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.209"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(235..597)
                     /locus_tag="MBZ71_002597"
     CDS             complement(235..597)
                     /locus_tag="MBZ71_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430494.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(671..1051)
                     /locus_tag="MBZ71_002598"
     CDS             complement(671..1051)
                     /locus_tag="MBZ71_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBT4430495.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1115..2308
                     /locus_tag="MBZ71_002599"
     CDS             1115..2308
                     /locus_tag="MBZ71_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4430496.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2385..2969)
                     /locus_tag="MBZ71_002600"
     CDS             complement(2385..2969)
                     /locus_tag="MBZ71_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4430497.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          924 a          570 c          493 g          985 t
ORIGIN      
        1 gttaaattta tcatgtattt cttccttggt caactataaa ttaagcatta ctttgtgaaa
       61 tgatttttcg atcaattatt attattcgtt atacaaccgt tttcaacata ttttgaaaat
      121 aaaatgcact aaaaaactat ctttagcaga acatttcatt gtattttttg gaccaaacaa
      181 taaaaaaaga agtttgaaaa aaattcaaac ttctttgttc gcttaatctg tattttagaa
      241 gaaaggcact ccctctactt cgtaagttcc aagaacagag cgcatttgac ctggttcttc
      301 ccaaatatat ccttcgccta attcttctct ttgattcttt tgttcagttt caaagacaaa
      361 caacggaaca ttttcctctt taatatgtgt atttgtcaat tccatcaaac taatcttgct
      421 aacatcgata tgaactattt cttttaagaa attaagtata ttcgctaaac cagtctttcc
      481 ttcagaaagt tccgctaacg caaattccaa cttgtcatca attgaatgga ccaaaaattt
      541 tttagtacca tcctccaaat gcaacataat tgtaccagca actcttttgc ttgccaaaat
      601 ttccacctcg atcaacttgt tctatggctt acataaaaat tctagcacat tttcagaaaa
      661 aatacacttt ttatttctta ttttctttcc gttctagttc agataatgct tcttctatcc
      721 aaataggtaa ctgctccgca atagtggtaa atccgatctt acgattttta ttcgtgaaat
      781 acctttttct gcgatatgga acagctggat ttgttttttc taacgttctt aaagataaac
      841 tgattttttt ggaatattca tcaatgtcga tgatctgaac tgttactggc tgtccgactt
      901 ttaacaatga atggatattt ttcgtataac cggattgtac ttctgaaaca tgaataagtc
      961 cttgcgtctc gttatcaaga gaaacgaatg cgccataagg ctgtatccct gtcacttttc
     1021 cttcaattat ttgtccaatt ttataagtca tctacttccc tctcttcgca tttatcatta
     1081 tactgtaaat agctcaaaaa aagaagcgac gaaaatgact ggaggtctta agatgatcga
     1141 atttgataaa cagatttcaa gaaaagatac agaaagtgta aaatgggacg caattgctga
     1201 gacatatcaa atggatgact tacttccttt gtgggtagca gatatggact tcttgtctcc
     1261 cggtggggta gctaaagcgt ttagtgaata catcaaacat ggaatctttg gttatgccac
     1321 tctgtctgat aaactatatc aggctatcat tcattgggaa cgccaacacc atgctgtgga
     1381 gttgacgaaa gaaaatatcg tttttacaag tggtgtgtta accagtttag ctgcagcagt
     1441 acaaactttt acaaaaccag gcgattctat tttgattcat gacccagttt atcctccatt
     1501 ttcctctatt atagaaacaa atcaacgtca catcgttcga agctctttgc tagaaaaaaa
     1561 taatcatttc atcatggact tgtctgacat ggagaaaaaa atcgaagaaa acaatgtaaa
     1621 agcaatgatt ttatgcaatc ctcacaatcc tggagggcgt gtttggagca aagaagaatt
     1681 gaaacagtta agcgaattgt gtttaaaaca tcaagtgatt ctgtttagtg acgagatcca
     1741 tcaagattta actttattcg atcatacatt tacttccatg ctaacgattg atcctaattt
     1801 agatgatcta ctagttgctt ttacttccgc aaccaagact ttcaatctag ctgcaatcaa
     1861 aaattcaatg gtatttatca aaaatccaga gatgaaagct gattttgaaa aacatttagt
     1921 gatgaaccaa cagcacgaaa tcaacacttt tggactgatt ggaacccaag cggcttatga
     1981 gacgggtgga gaatggttaa gccaactgat tccttatcta gaaaaaaatg ttcaaacagt
     2041 taatcagttt tttgaacagt atcttccgaa agtgagaata atgaaccctg aaggaactta
     2101 cttaatgtgg ttagatttta ccgcctatat ttcagacgac caagaattgg agaacactct
     2161 tgttcggaaa ggaaaagtcg tattaaatcc tggcatcacg tttggtcctt ccggtcatgg
     2221 gcatatgcgc ttgaatattg cttgtccaga agaaacttta ctggaaggac ttaatcgaat
     2281 caagtatgct ttagagacaa tcgattaaat catttattca aaataaaagg acgagacctt
     2341 actaggtttc gtcctttttc ataattactc cgtaccatac caaactattc tgaaatttca
     2401 attgtttcaa tcactacatc atgtacaggt cggtcttgtg gaccacgttg tacatttgcg
     2461 atttcatcta ctgtgtccat cccttctagt acatgtccaa agactgtatg tcggaaatct
     2521 agccaaggag ttccgccacc tttatacgct tcaatgattt ctgatgggaa tccagcacct
     2581 tctagttgtc caagcatatt agctggcaca tttgtgttat ttacaataaa gaattggctt
     2641 ccgtttgtgt ttggacctga atttgccata gataaagctc cgcgaaggtt gaacagttca
     2701 cgactgaatt catcttcaaa actttcgcca taaatacttt cgccacccat tcctgttcca
     2761 gttggatcgc cgccttggat catgaaatca ggaataacgc ggtgaaaaat cacgccatca
     2821 taataacctt ttttggaaag ttcaatgaaa ttttgaaccg tcttcggtgc tagttcaggg
     2881 aataattgaa cagtaatgtc accacggttt gttttgataa cagcttttgg tcctttttca
     2941 ctttttaaat ctagttgtgg gaatgccata tt
//
LOCUS       DAGCWU010000204         2949 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.210, whole genome shotgun sequence.
ACCESSION   DAGCWU010000204 DAGCWU010000000
VERSION     DAGCWU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.210"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(50..592)
                     /locus_tag="MBZ71_002601"
     CDS             complement(50..592)
                     /locus_tag="MBZ71_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBT4430498.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            727..1116
                     /locus_tag="MBZ71_002602"
     CDS             727..1116
                     /locus_tag="MBZ71_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430499.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1120..1884
                     /locus_tag="MBZ71_002603"
     CDS             1120..1884
                     /locus_tag="MBZ71_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBT4430500.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2013..>2949
                     /locus_tag="MBZ71_002604"
     CDS             2013..>2949
                     /locus_tag="MBZ71_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4430501.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRE"
BASE COUNT          940 a          496 c          611 g          902 t
ORIGIN      
        1 taacatttaa aatttaattt agatattaga cagtttttaa gtttgtgagt tattttcctt
       61 gtcgttttct cactttttta gtccagaaac cacctgaaat atattttggc tcataagaaa
      121 tgataaaagc acgctcttcg atttctttga tcaggcgata cagttcacgt tcgctttttc
      181 taggagaaag tatttcgaga actagccgct caccttccag ccccattgca gaagtctgtg
      241 tcacaccata accgtgttct ctcagtatac ccggcaaact tttatcttct gcgctatttg
      301 ctggcaaaat cgttgtgacc atgatgtatc ctaatgccaa tttgtcttca atcaaaattc
      361 cgacactgat acctactgcg taacctaacg catagacaaa taaattcagt ggattatcta
      421 aacgatctaa aacaagggat aagccgacga tataaatcgt gatctcgacc atactgacaa
      481 gtggagcaat cagtcgatag cctttcattg tcaacatgaa acgtaacgtg ttcagagtga
      541 tataagcaaa attaatgagg aagatcatcc ccaacatttt tggatcaatc atttgtaaaa
      601 ctcctttcat ttatataaca acaatatcaa aaaaaatcgt aagtgaagaa aaattgaaac
      661 tttatacata caaaaaaatg ataaaatgat tataatagaa ttataaagat aggttaggtg
      721 attaaaatga aaacggtcaa tcgtgtgttt tgtgccagcc acgtgctaag tctccttctc
      781 agtatatact taggtgtcta ttcgattcaa cagccaattg aggtagagaa cattgttttt
      841 cagatgtcgc tgacagatgg tttaatttta agcgtttttt tctttttgat actgtttatt
      901 ggtaatattt tcggtagcgt tgccagtttt ttgaattttt ctatttatcc actcctttcg
      961 ctagcattag gagtggtggg actttgttct ctgtgcttat ttcccgagcc attcagtcct
     1021 gcatttatat tatttggtac actgaacctg ttccagacaa ctgttggagc atggttgcta
     1081 tggcgatcag gaaatttaat gaaaataggt gagtaacaat tgaatccaat tatattgaaa
     1141 cgttctgtcc atcatattcc agtgttagaa gttgttctga aagaattaag agaggaacgt
     1201 attcccgttg tcatctatta tcacggatgg cagacaagta aggagttagt tttgacgcaa
     1261 ggaagaaaaa ttgcgcagca gggcattcgt gtgatcctgc cagatgcgat gaatcatggg
     1321 gagcgcaaac agccggtttc ttctattcca tcctatacat tttgggatag tatctacgga
     1381 aatctgtttg aatttgacac acttatcaag catttagaaa aaagaggtct tctacatgat
     1441 cagcttgcag ttggcggggt ttcgatgggg ggaatgacaa catgcgcctt gttggctaaa
     1501 catcctcata tagtagcggg cgtgtgtttg atgggttccc ctgcaccttt ggaatatgga
     1561 aaagaaatcg taaggcgcgc atcggaatat aattatgtcg taccaaaaga ctattttgat
     1621 ttgatttcat gggttaagag ctatgatttg tcattgcagc cggaaaagtt agctggacgt
     1681 cctctgttct tttggcatgg acaagctgac gaaaaaatcc cttatataca aacggaacga
     1741 tttgtagaaa aaaatcggca taagtcttac ggaaaagaaa ttgtttttcg ttcctcaaaa
     1801 acacagcgac atatggtaca aataccacta atggaagaag cttccacttt tttagcaaaa
     1861 aatttgataa aaaagtataa ataattgaaa cggaaaatca ctcatgacga tagaggattt
     1921 tccgtttttt attttgggct ataataaact ctgttaggaa aatttggtat aattgatagt
     1981 aagaatgaca acatgttaga aaggaagaga gcatgcccac tttagcagat gttgcaaaac
     2041 gtgccaatgt ttcaaaaatg acggtctctc gagtcatcaa tcatccggaa caggtgactg
     2101 acgaattgaa agagttagtt ttctcagcaa tggctgaact ggattaccgt ccaaatattg
     2161 ctgcaaaagc acttgtcacg aatcggtcgc agatcatcaa attatttata ctggaagaaa
     2221 tcgacacaac tgagccctat tacatgaatt tgctgatggg aatcgctaaa cggatcggta
     2281 aagcgcatta ttctctccaa ttagttacaa atgatgcctt cgacgtcggt tcttgtgatg
     2341 gatacatcat cacaggtgtc agaaaaaaag acttcgattg gatcgaccgt ttggaaaaac
     2401 cagttgtctt gtttggggaa aattctgaag gatatgatta tgtagacagc gataatgaat
     2461 atggtacagc aaaagcaacg caatatgcct tagaaagaga ttatcaaacg attatttata
     2521 tcggtatcga agtggacgag ccttttgaaa tttcaaggga aactggttat ttatctgtga
     2581 tgaaggaagt tgatcttcct agtaaaatct tgcggtttcc aaatcattcg acagaagtag
     2641 aaagatatat gacagaaaac tgggcagatt ttacagataa aacttgtttt gtatgtagtt
     2701 cagatcggct agcactagga gcagtaagag ggattataaa tgctggagga cacttgcccg
     2761 aagaatttgg tgtgattgga tttgatgggg tatttttaga ccaaatcagt tcacctaaat
     2821 tgacaacgat caagcaagat attatacgta tgggtgaagt atgtggagaa atgttgttaa
     2881 aaaaaataga agaaaaaggg gcgagtcaag gttatcgtcg tttttttcct gaactagtta
     2941 tcagagaga
//
LOCUS       DAGCWU010000205         2949 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.211, whole genome shotgun sequence.
ACCESSION   DAGCWU010000205 DAGCWU010000000
VERSION     DAGCWU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.211"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            104..2166
                     /locus_tag="MBZ71_002605"
                     /pseudo
     CDS             104..2166
                     /locus_tag="MBZ71_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
     gene            2249..2818
                     /locus_tag="MBZ71_002606"
     CDS             2249..2818
                     /locus_tag="MBZ71_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4430502.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1058 a          531 c          569 g          791 t
ORIGIN      
        1 tctcctaata gtccatcttc ttttgatttt atttatataa tgaaatcatc ttatgaaaat
       61 tactacttca tgtttagaca agagtaggga ggactaagaa taaatgtgtt caaaaagacg
      121 ttcaaaacaa ctaaataaac acgcttgtcg tttagataag gaacggtcgt gttctgaaaa
      181 acactacgtt aagtttatga tgcgcacgat gctgatcagt ttatctgtag gtgtctatat
      241 gcatgtaccc gttcaggcaa cgacacaaac catcaaggtc gtacgtacct ctgtttctga
      301 gaccactgat tcccctatca ctattactca tactttcact gatgcaaatg gaaaacagct
      361 acttcctgca ttgacttcct ataataaagt cagttatcct ccaacgataa accactactg
      421 gttaattcct aacaaaacat tatggacatc ggaaaccgga agcagtgtgc cgctttctat
      481 cttttccgat atgactgatc ctacaaaagg tgatcagtta aaacagatca ctgatatttt
      541 gaatcatgct ttgacagata taaaggacgg tggaagcgca caagtttctt atgtatatga
      601 aaaagattat tcacagtttg aaagaaagaa aatcgatgtt ctggcaggga caaaagtttc
      661 gatatctgat ctggtagaaa ggctgaagaa cgcagatgga acacaaggag atatgtccaa
      721 agtaatttca aacaatggga atatcgtgga tacgactaat acaggaacat ctactatttc
      781 cttgtcctat tttgatccaa ttgctttaaa aacaatgact gcatctgctg tgatccatgt
      841 tcgtgctgat caaacagcta tcaaaggaaa agatcttcac ttaagaacag gagagagtta
      901 cgcccctaaa gatcttgttg aatatgtaac aaaaagcgat ggaacttcag gaaataaaga
      961 agaaatcaaa atgaacggga cagtagatac tacaactccg ggagaatatc ccgttgtatt
     1021 aacttatata gactcgaaaa catcaaatgt ggcagaaaca acagctgtag tttatgtaga
     1081 agcatctgct gcttcatcga ctgtcatcac tcattttata gatagtcaaa caaataaaga
     1141 acttcaagca gaaacacata tgagtagtgt aaatcgtatt cctgatttgc gcctacctgc
     1201 tgaattttct gatcgagtgc caaatatata gatttatcga cttatgaaca aaatgggaaa
     1261 gtcacttcta tttctgattg gatgcaagca aacggattag acagaaaaag tcactggtcg
     1321 gagatccttg aagtacttca ctcagaactg atgaataata ggaagcaatc aggaggggaa
     1381 attaccttta cttatgttta tataccagac aatcgttcat tagaaggtca tgatcttatt
     1441 gttcatccaa acgaaaaaat aaacaaagaa gagttgatcc aatcagcaac aaattcctta
     1501 ggtaaacccg taccgttaaa ggatgtatcg gttacaatca atgaagtacc attggaggat
     1561 ggttatttga ccaaacaaac aggtgatttt acgattatgt atagttatca tgacccgtat
     1621 tcactccaag aactccatgc tcagtcacta ttacatgttt taccattaaa caaacagata
     1681 gatcaaaaag aacaaaaaag acaagaagaa agcttgtcag aaaaaaaaca acaaatggct
     1741 catgcagttt ctccaacagg gaaagtaaaa caaacaagaa gcctagagtt agcaagaata
     1801 tctagaaaaa tctcagaatc taataaaacg ataaggcaag aagctcattt ggccccacaa
     1861 gtttctggat tggaaaatga aattttagag cctaaaataa aacaacaatc agaaaaagaa
     1921 tttgttgcta aacagtcgga agatcagaag gaatcagatg accagaagga aacgaagaaa
     1981 aaacgcttgt caactattga ttccaccgat gagcatctgc agccgcaagt accacttcct
     2041 agtccgggaa cacccgcacc tggtggaggt attccttctt ctgctttagg agatttcatg
     2101 cgaggtatgg caggaacatg ggtatacgct aatcgaaatg aatttgacgc agatgactgg
     2161 ttctgattta tatctctatc tgttatagtc aaataagtag ctgagtcata tgttatttgg
     2221 cataatggag acgaggaggg atagaaaaat gttagataaa acgttacctt acgcagaaat
     2281 ctggatgaca agaccacttc aacaaaatgt tccaatcacg cctttagcgg aaggttttga
     2341 atttcgattt tatcaaaaag aagatgaatc tgcttgggcg gagattgaga cttccgttgg
     2401 ggaatttcaa acgacagatg aagcacttga gtattttcaa caaaactttg ctccttatcc
     2461 tgaagaactt gaaagaagga tgttttttgt agagacaaaa aaaggagaaa aagtagcaac
     2521 ctgtaccgct tggcgaaaaa aatcaagaga gcaagtgtat ccggtttttc attggttagc
     2581 agtgaaacca gattaccaag gaaaagggct cgcaaaagca ttgactgcca gagtcttgca
     2641 agaatttcct accctccata cggacggacc aatctatctt cacacacaga catggagcca
     2701 tcaggcgatt gctctttata aaaaaatggg ttttactttt attgctgaaa atctagacgg
     2761 cacacctaat ccagattatg aaaaagttat gcaggtttta agtaaattag ataaataacg
     2821 aaatggtgaa ataagtcctc agtattggga taaaaaacga aatagtcatt cctcatatct
     2881 tacttttttg agttaatact atcggataac taatggcatg ccatacattc aaaaaagaag
     2941 gggcagtga
//
LOCUS       DAGCWU010000206         2945 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.212, whole genome shotgun sequence.
ACCESSION   DAGCWU010000206 DAGCWU010000000
VERSION     DAGCWU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.212"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            6..977
                     /locus_tag="MBZ71_002607"
     CDS             6..977
                     /locus_tag="MBZ71_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBT4430503.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            970..1998
                     /gene="vanB"
                     /locus_tag="MBZ71_002608"
     CDS             970..1998
                     /gene="vanB"
                     /locus_tag="MBZ71_002608"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBT4430504.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2004..2612
                     /locus_tag="MBZ71_002609"
     CDS             2004..2612
                     /locus_tag="MBZ71_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBT4430505.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(2678..2851)
                     /locus_tag="MBZ71_002610"
     CDS             complement(2678..2851)
                     /locus_tag="MBZ71_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430506.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT          780 a          625 c          812 g          728 t
ORIGIN      
        1 aaccaatgag aaaaagtgtg ggcattactg tttttggatg cgaacaggat gaggcaaatg
       61 ctttccgcgc tttatcgccg gattttcata ttatccctac gctgattagc gatgcgatat
      121 cggcagacaa cgcaaaattg gccgctggca atcaatgcgt tagcgtaggc cataagtccg
      181 aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg ggtaaaatac atttctaccc
      241 gcagcatcgg ctgcgatcac attgatacga ctgccgccga gagaatggga atctcggttg
      301 gcacggttgc gtattcgccg gacagcgttg cggattatgc tttgatgctg atgctgatgg
      361 ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca acaagatttc agattggatc
      421 gtatccgggg gaaagaactg ggggatatga ctgtgggagt tattggaacc ggccatatcg
      481 ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca tgtgctggcc tatgataaca
      541 gccgaaaaat ggatgcagat tatgtccagc ttgatgagct tctaaaaaac agcgatattg
      601 ttacgctcca tgtgccgctt tgtgcggata cccgccatct gatcggtcag aagcaaattg
      661 gagagatgaa gcaaggcgca tttttaatca acaccgcgcg cggagcgctt gtcgataccg
      721 gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg gatgtgttgg
      781 agggcgagga tcagtttgtt tataccgatt gctcgcagaa agtgcttgac catccctttt
      841 tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc ccatacggcg tactacactg
      901 agcgtgtgct gcaggatact acagaaaaaa caatcaggaa ttgtctcaat tttgaaagga
      961 gtttacagca tgaatagaat aaaagtcgca atcatcttcg gcggttgctc ggaggaacat
     1021 gatgtgtcgg taaaatccgc aatagaaatt gctgcgaaca ttgatacgga aaaattcgat
     1081 ccgcactaca tcggaattac aaaaaacggt gtatggaagc tatgcaagaa gccatgtacg
     1141 gaatgggaag ccgacagtct ccccgccata ctctccccgg ataggaaaac gcatgggctg
     1201 cttgtcatga aagaaagcga atacgaaaca cggcgtattg atgtggcttt cccggttttg
     1261 catggcaaat gcggggagga tggtgcgata caggggctgt ttgtattgtc tggtatcccc
     1321 tatgtgggct gtgatattca aagctccgca gcttgcatgg acaaatcact ggcctacatt
     1381 cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa ttattgataa aggtgacaag
     1441 ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc aggttcgtcc
     1501 tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga agcggcagga
     1561 caatatgatg gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt cgggtgtgcg
     1621 gtcatgggga acgaggatga tttgattgtc ggcgaagtgg atcaaatccg gctgagccac
     1681 ggtatcttcc gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa tgcgatgatt
     1741 acagttcacg cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac ggcaaagaaa
     1801 gtatatcggg tgcttggatg cagagggctt gcccgtgttg atcttttttt gcaggaggat
     1861 ggcggcatcg ttctaaatga ggtcaatacc atgcccggtt ttacatcgta cagccgctac
     1921 ccacgtatgg tggccgccgc aggaatcacg cttcctgcac tgattgacag cctgattaca
     1981 ttggcgttaa agaggtgata agtatggaaa atggtttttt gtttttagat gaaatgttgc
     2041 atggcgtccg ttgggatgcc aagtacgcca catgggataa cttcacagga aaaccggtgg
     2101 atgggtatga ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct ctgcgcgagg
     2161 cacaaatcca tgcggctgcg cttggctatg gcttgctttt atgggatgga tatcggccaa
     2221 gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc ggaggacaat ctcacaaaag
     2281 aaaaatttta ccccaatata gagcgagccg agttgattac aaagggttat gtggcctcac
     2341 aatccagcca tagccgtgga agcgcaattg atcttacgct ctaccacctg gatacagggg
     2401 aacttgtttc aatgggaagt aacttcgatt ttatggacga acggtcgcat catacagcaa
     2461 aagggatagg ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc atggaaagca
     2521 gcggatttca gtcttatcgc tttgaatggt ggcactataa gttgattgat gagccatacc
     2581 ccgataccta ttttaatttt gccgtttcat aatgaaagta ttggttttgt atcagatttt
     2641 ggatttgata aaatctatgc tggtcatgga caatctttta ctggaaagtg gttaaagcat
     2701 cttttgtaat acattgcaaa ttatagtatt caatctgccc agcggcagaa aagaaaaaaa
     2761 ccgctgttgg gcagaccttt cccacgccca ttttgtattt tgctggcggc ggttttgaga
     2821 aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg cggcccatag gaggatgccg
     2881 ctatgctgtt ttgctattgc catgctctaa tccacttcca ccactcaaaa aagccttgcc
     2941 cctcc
//
LOCUS       DAGCWU010000207         2866 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.213, whole genome shotgun sequence.
ACCESSION   DAGCWU010000207 DAGCWU010000000
VERSION     DAGCWU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.213"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(17..835)
                     /locus_tag="MBZ71_002611"
     CDS             complement(17..835)
                     /locus_tag="MBZ71_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBT4430507.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            1000..2730
                     /gene="spxB"
                     /locus_tag="MBZ71_002612"
     CDS             1000..2730
                     /gene="spxB"
                     /locus_tag="MBZ71_002612"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBT4430508.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
BASE COUNT          910 a          604 c          555 g          797 t
ORIGIN      
        1 tatatatttt ttttatttat cctaatgcca catccaaaat catcataatg ataaatccac
       61 tcatgacgcc aaaaactgca aaatgacgtt tgctggacaa tgtctgctgt gcttcaggga
      121 tcaattcttc caccacgaca taaatcattg ctcctgcggc aaaagcaagt gcataaggaa
      181 gcagcagctc cactttcgtt actagtagcg ctccaatcac tccagcaatt ggttcaacga
      241 ttccagaagc ttgtccgtaa acaaaggcct ttttacgact cagaccttct tgtcgcaacg
      301 ggattgatac agctgctcct tctgggaagt tttggatacc aatacctagc gccaccgaaa
      361 cagcggctaa tatagcggct gtcggattat ccgcagttgc tgctgcaccg aatgctaccc
      421 ccacggctaa accttcagga atgttatgca atgtaataga aaaaacaagc aagatcgtcc
      481 gttttaaatg ggtaggcaat ccttctgctt catgttgtgg tccaaaatgc atatggggca
      541 gtgttttatc tgccatatat aaaaacacac cgcctaaacc aaacccaata cttacaacta
      601 gccaagcaat atctccattt tcttctgctt ttgtaatagc cggatctaac aaagaccaaa
      661 aacttgcagc aatcatgaca ccagaagcaa atcctaacat caagtttagc acttcttttt
      721 tgatttcttt aaagaaaaaa accaacccgg caccaagagc tgtcataaaa taagtaaaag
      781 ctgtcccagc aagtgcttgc tgccaaggtt caagtctcaa tagccattca accatgccat
      841 tcccctctct tgaatctatc tatgtactac tctagcatat tcccaaaaac atgactacat
      901 ttttgtgaaa agacgaatta gttgggattt tcgataaaaa gttctatact ctagatacaa
      961 aaaatttatg tatcatcatt gctaaaggag tgaaacacca tgtcaaaaat caatgcagga
     1021 atcgcaatgg taaaagttct tgaaagctgg ggtatcgacc atatttacgg cattcccgga
     1081 ggatctttca attcaattat ggatgcgcta tatcaagaaa aaaccgaaat caactacatt
     1141 caagttcgtc atgaagaagt cggcgcactc gcagccgctg cagatgcaaa attaacgggg
     1201 aaagtcggcg ctgtcttcgg ctctgcagga cctggtgcga cacatttgat caatggtttg
     1261 tatgatgcac agatggacca tgtgcctcta gtagcgttct taggacaagt tgcttctaca
     1321 tccatgaact ataactcttt ccaggaacta aatgaaaatc ctattttcgc agatgttagc
     1381 gtatacaatc gtacagtcat gacaccacaa agtcttccgc atgtagtaga tgaagccatt
     1441 aaagccgcct atgagcataa aggcgtggct atcgtgacta tccctgttga tcttggtttt
     1501 gaagaaattg acgaacaacc tttctccacc gctcacactc acaaaacaag tgtgatatta
     1561 ccagaagaaa aagatttgct agctgctttg ccttatcttg aaaaagcaaa aaaaccagtt
     1621 ctttatgttg gtcaaggaac gcgaaacgga tttccgcaaa tcaaagaatt ctcagaacat
     1681 ttttcagttc ctgttgtcgc ttccgttcta gcaaaaggaa ttattcctga tgattatgaa
     1741 aactttcttg gttttgctgg acgtgtcgct actaaacccg gaaatgaagc attggcagaa
     1801 gccgatctga tcttatttgt cggtagcgac ttcccatttg gacgtgcatt ttttaatcca
     1861 actgctgaat ttatccaaat cgatatcgat gcgtcaaaat ttggacgacg ccatgaaaca
     1921 tctttgtcga tcctcggtga tgcaaacaca gctttgcaaa gacttgtcga gttaggggat
     1981 gctcgccctg ctgatagctg gtacctagca aatcaagaaa ataaagaaaa atgggtaaat
     2041 tggttgaaat cttttgaaga tcgagaggaa gaaccgattc gtccagaggc tgtctataaa
     2101 gaaatcaatc gtattgctga agacgatgcg attttcgtca cagatgtcgg taacacgact
     2161 atccattcta ttcgtttatt gaatatgaac ggtaaacaaa aacatacgac atctggatgg
     2221 ttcgcaacta tgggtaatgg tgtgccagga ggaattgccg cacaactaag ctacccagaa
     2281 aaacaagtat ttaccttaag tggtgatggc gggtttgcta tggtcatgca agatatcatt
     2341 acacaagtca aatatcagct tccaattatt aatgttgtct tttcgaatga ttcttttggt
     2401 ttcatcgaag cagaacaaga agatacggaa caaaagaaat ttggtgtatt tttagaagat
     2461 gcagatttcg gtaaagttgg agaagcgctc ggggcaaata gctttacagt cacagaatac
     2521 tcgcaacttc gatttgcctt tgatgcagct gcaaaatcag atcgtcctgt agtaatcgat
     2581 gtgaagatca ataacaagcg cccattgcca gtagaagatc tgcatttaga tccaagaaaa
     2641 tattctgaag aagaaatcca agcattcaaa gaaaaatatg aagtccatga tttgccagta
     2701 ttgaatgaat tatatgaaaa gaggttgtga caactgcgct atgcttcgag tattaagata
     2761 aaatttgtgt atatagcttc tcacattttt gcataaattt gtctcgacac ctaaaaatca
     2821 cctcgtgcat atttgtccat gataaaaaag ccggcttcca attaca
//
LOCUS       DAGCWU010000208         2799 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.214, whole genome shotgun sequence.
ACCESSION   DAGCWU010000208 DAGCWU010000000
VERSION     DAGCWU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.214"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(136..630)
                     /locus_tag="MBZ71_002613"
     CDS             complement(136..630)
                     /locus_tag="MBZ71_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430509.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(650..937)
                     /locus_tag="MBZ71_002614"
     CDS             complement(650..937)
                     /locus_tag="MBZ71_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430510.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNK"
     gene            complement(1153..2544)
                     /locus_tag="MBZ71_002615"
     CDS             complement(1153..2544)
                     /locus_tag="MBZ71_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4430511.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(2529..>2799)
                     /locus_tag="MBZ71_002616"
     CDS             complement(2529..>2799)
                     /locus_tag="MBZ71_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430512.1"
                     /translation="IFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYF
                     EYWIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
BASE COUNT          810 a          528 c          385 g         1076 t
ORIGIN      
        1 agttgctcaa actcttcctt atttatcact aatgtttcaa acggaacaac tttattttcc
       61 tcagccattc tcccagttcc attttcaaaa atcacaaaac tgatattacc ttaaaaacta
      121 ctaacaaaat aaaacttata aagtaaatgg caacccaact atgtaaccat tctctctacg
      181 tactacgatt tggtagtcct gcctttcaat cggtaattca taatagtaat ttactgtttc
      241 ttctttgcca ctttcaatat actctggatt atccaatatc atatcatact cataaagttc
      301 ttcaccatcc ttattttcaa tcgaccattg ttgtggacgt atcggatttt ctgtatcatt
      361 tataagtgta taggtcactt gtaatacacg atcatgttgt gcaaattcat ttcttttttc
      421 ggtcacttca atatttttaa atgttagttc aaccccatct gcctgataag tttcacctat
      481 ttttaaatct tttgttacaa tttttattgg cttttcttct ggaaaatgtc tggctttaat
      541 tgttagataa cttccaaaaa gaatcaatcc tagtagcact aaaagaattc ctttaacgag
      601 ccactgaaac gggaagtaaa ctttaaacat cttttttctc ctccgttttt tatttgtttt
      661 ttagagaata cgataacttt tgatcaatgt catccagctt ttcaattact tcattactta
      721 tcattgattc tagttctgaa attttttgtt caaatttaat gctttcagga tcattaaatt
      781 tattatttga tggactagct aacactggta aattcgaaac cataacacga aacatatttg
      841 cctttgaacc aaaaactcct ttgttttggt tgtaaaaatt gttgaatatt tctttagttt
      901 cttctgaagc cgagacttta aaatatggat agttcatttt tttcttccac ctttcttttt
      961 ttgggaggga gtctgtaggg agcttgctcc ctcagagatt tacccaccat ctgacttgcc
     1021 agaaagtagc ctagcgtgag cggggcggat tgactggcaa gtagatgggt agctggcgta
     1081 tgtcctaaaa actgcttctt tccaagcaac tcttcatagg caaaaaactg gaaagtccac
     1141 taacaagccc attcagaatt ccatttcaaa attttgttgc cttttttgct gattttcaat
     1201 catttgttgt gtgaattcat aatccttttt agcacgatac atttttacat cgtcattcat
     1261 tatgcgactg atttgatagt gaatctgttt agcagaaggt gtatgagaac aattttcaaa
     1321 accctttgcc ggtagaaata actctttctg atcagtcttt ttaatagaaa ttgttttact
     1381 tttcgcacta cgtaaagttt gaggattcac atccgtatga agtttaatcg tgtattcttg
     1441 atcatcttct aaagacattg tatttacaaa atgcttgtct acctctcgca agaagtcatc
     1501 atatttaatg aatttttcat cgccttcacg gtcgctaaat tgcttttgtg cgatggaatc
     1561 taattccaca aacggtacat ttaactcacc agttttgtaa tttactccac taattagcga
     1621 cataacttgc tctctttcca tattaaaaaa attttttata tgtgattctt ctaaattctt
     1681 ttcaagttgt tcagaaacag attcaaatga atatcttttc cctaactttc ttgcactaaa
     1741 agtacgttcg acatactgac ttttttcttt tctagattgt ttatctcttc ctaaagtaat
     1801 taactcacct ctacgctcat agaaatttac accttgtttg taaagaacat agccaagttt
     1861 gttatttgtt tttgaaaatt gataaacttc aatgttatat tcttttgcca aatgatccga
     1921 aaaatcttca aaagaattca cttcgcattt ttccaacgtt gcttcgatcg cattttgcac
     1981 aaactcttga taattaattt ttccatattt catcttagaa ggatcgtttg aataaagatt
     2041 ggttctcttt tctaagtttt catagtactc attattcact gattcaagac catgttcatt
     2101 gaggacttga tcgttaattt ttcgcattag ttctaaatct ttagggtcaa tacgcaacct
     2161 tttacatgtt tccatgttaa tattattaat gacaagatga ctatgaaaat gatctttatc
     2221 attgtgactt gcaacaatga tttgcgaatt tggaaaagct ttctctgcaa attcgacagc
     2281 tatttcgtgc attttatcaa tacttaactc tttagaagta ctcttatcaa acgaatgaat
     2341 aacatgaaac gcttgtgcta tatctttaga tttattcatc ttggtacgta ctaaattcat
     2401 ttcttgcagt gcttcctcac tatcatcact acaatttatt cctgagactg caacacattt
     2461 ttcttttttt gaaggattag ctgcatattg taatagcgag gataaatttt tcttgcgtcc
     2521 tttcgatgtt atagtagctc ccaaagttgt ttcacctcat cttttaaact tgcaataatc
     2581 tgaattgagt gaattaaatt ccgatcaact ttttgggaat actcttcttt cgtacccggt
     2641 ctagtttgct tttctatcca atattcgaaa tattcttttt ctacttctga taatttaaaa
     2701 aaagaggggg cactccgatt ggactccaaa aaaatgtcat ctggataact aatcatcaac
     2761 ttttttgcta tgctattcgc aagttcatct ttaaaaatg
//
LOCUS       DAGCWU010000209         2797 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.215, whole genome shotgun sequence.
ACCESSION   DAGCWU010000209 DAGCWU010000000
VERSION     DAGCWU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.215"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            55..999
                     /locus_tag="MBZ71_002617"
     CDS             55..999
                     /locus_tag="MBZ71_002617"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBT4430513.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1666..2064)
                     /locus_tag="MBZ71_002618"
     CDS             complement(1666..2064)
                     /locus_tag="MBZ71_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBT4430514.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(2061..2297)
                     /locus_tag="MBZ71_002619"
     CDS             complement(2061..2297)
                     /locus_tag="MBZ71_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4430515.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(2457..2741)
                     /locus_tag="MBZ71_002620"
     CDS             complement(2457..2741)
                     /locus_tag="MBZ71_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430516.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT          956 a          451 c          471 g          919 t
ORIGIN      
        1 agactatact tttcatgaaa gtaacaaata gttatgaaaa ggagctgttt attcatgaaa
       61 aaaacaagtc gtaaagtagt aatcgtcggt acaggttttg ttggaacaag tatcgcttat
      121 gcaatgatca accaaggaat ctctaatgaa ttagtattga tcgatgtgaa tcaagaaaaa
      181 gcagaagggg aagcattgga ccttttagat ggtatggctt ggggcgatga aaatgtggct
      241 gtatggtcag gtggctatga agagtgtaaa gatgctgata tcgttgttat cacagctggt
      301 atcaaccaaa aacctggtca atctcgttta gatttagtta aaacaaatgc gtcaatcatg
      361 cgccaaatcg taaaagaaat catgggatca ggattcgacg gtattatcgt tgttgcttca
      421 aacccagtag atattttgac atatatcgct tggaacgaat ctggtctgcc aacatcacgt
      481 gttatcggta ctggaacaac attggataca actcgtttcc gtaaagaaat cgcattgaaa
      541 ttaaaagttg acccacgcag cgtccacggc tatatcttag gtgaacatgg tgattcagaa
      601 gttgctgcat ggtcacatac aactgtcggt ggtaaaccag tgtttgaaat tgtagaaaaa
      661 gaccatcgta ttgcccaaga tgaattagat gtgattgctg ataaagtccg taatgctgct
      721 tatgagatca ttgatcgtaa aaaagcaact tactatggta ttggtatgag tactgcacgt
      781 atcgtaaaag ctatcttgaa taacgaacaa gctgtattgc ctgtatctgc ttatttaact
      841 ggtgaatacg acgaaaaaga tatctttaca ggtgtgccat caatcgttga cgaaaatggt
      901 gttcgtgaag ttgttgaatt atcaatcaac gaagaagaaa aagcaatgtt cagcaaatca
      961 acaagtgctt taagagaagt attgaacact gtattataag aaacaatgaa tatgaactaa
     1021 aaatcctaaa actgcttaac agctgaaata gctgttaagc agttttttat tgatatctaa
     1081 taggtaagca aataaacaga atggttaaaa ttaatcagtc caaaaatgta tccgtacata
     1141 tgaacatatg aaatttaatc tgttgattac aataagcgaa tcgttatcaa tgtttatctg
     1201 tggttaatta aaaaatcctt ctgcaacggc aaatacaatg attcatatct aattcgttca
     1261 tatttgatta actgaataaa gaaactggtt tttatcattc aattaaacga caaaaaaagt
     1321 aagaaaaact gcaaaatttt tttataattt tgcataagca gattttgatg gaaaaatgac
     1381 gatttttgat aaaaaaccat tcgagagagg tttatcattt tttgaatcta ctttgtataa
     1441 tatatattat gtaaactaaa atatataaaa aatcagccca ctagttctaa accactattt
     1501 cctactattc actcttttgt attcctttta aatcaattat ttcaaaatta atccatattt
     1561 tccatatttt tatgaaaaaa ctattttgta tgattaaaca taaaaaaatc agcatattcc
     1621 ttaatgacat gctgatctaa ttgagaaata aagtttttaa ttcgttcatt ttattgcggt
     1681 ttgtcgtaaa taactggtta tcttggactt tagtgaatcg aaatccaatg aactagtggt
     1741 aatcattatg ataaaatcaa cagcttcttg acgagaaaaa gcaactggac aattattcaa
     1801 ttgaagaaac aacaacatgg caacaagagc agttcgttta ttgccattat gaaaaggatg
     1861 ttttttagct aaattaattg ctaaaatcgc cgctttttca tagatttcag gatagagttc
     1921 ttgattgaat actgcttgtg aaggctgttt gacggccata tctaatgcat ttgcatcttt
     1981 tacaccgatc atttcgcctg gcgagtgacg taaaataact ttcgcgttga ttttgataat
     2041 ttctttagtc gaaagatacc tcataaatca accaaatctt ttaacgcgtc ttcatattga
     2101 ttcatgatcg aatcaactaa tgaatcaata tcaggttctt cttgtgcttt gatcattcgt
     2161 acagaaccat cagattgaaa aacataactg attgcgtcac ctgtctgcac acccatgaat
     2221 tcttttactt ctggtgggat agttgttaca tcgctattgc cagattttct aattttatac
     2281 tttttcattt ttttcatatt tcgccaccct ctataccatt ctcattctat agtaagtaca
     2341 ttatatccaa gtatatactt ttaatcaatg tatatacttt tattttgctt tttcttacga
     2401 aaaaatgaca aagaaaaaag tttgtgacat gacttttgtc acaaactctc gtattcttaa
     2461 ccaaaatatt gatgaaatac ttgtgtaata actggtagaa aataaataat gatcatagcc
     2521 gctaccacca ccaacaattg ttttcgctgt ttctttcgct ttagcatgct tgtcgtttta
     2581 tgaaaaaaat aagtagcgct tttagctcct gcagtcaaag tcgataaaat caacgttggt
     2641 gaatatgctg tttgattgtg cgtcaatgac aacaacttcg tcccgtaatc aggatacttt
     2701 agaaatgctt ggtacacagc cacaatcgtg attgctccca taagataatt taaaaatgaa
     2761 tgcacttttt tcaaataaaa aaactccttg ctatcta
//
LOCUS       DAGCWU010000210         2720 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.216, whole genome shotgun sequence.
ACCESSION   DAGCWU010000210 DAGCWU010000000
VERSION     DAGCWU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.216"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..2530
                     /locus_tag="MBZ71_002621"
     rRNA            <1..2530
                     /locus_tag="MBZ71_002621"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2625..>2720
                     /gene="rrf"
                     /locus_tag="MBZ71_002622"
     rRNA            2625..>2720
                     /gene="rrf"
                     /locus_tag="MBZ71_002622"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          766 a          564 c          815 g          575 t
ORIGIN      
        1 tggaagacac ctaggaggat cctgagtacg gcggaacacg agaaattccg tcggaatccg
       61 ggaggaccat ctcccaaggc taaatactcc ctagtgaccg atagtgaacc agtaccgtga
      121 gggaaaggtg aaaagcaccc cggaagggga gtgaaataga acctgaaacc gtgtgcctac
      181 aacaagtcaa agcccgttaa tgggtgatgg cgtgcctttt gtagaatgaa ccggcgagtt
      241 acgattgcat gcgaggttaa gttgaagaga cggagccgca gcgaaagcga gtctgaatag
      301 ggcgtttgag tatgtagtcg tagacccgaa accatgtgat ctacccatgt ccaggttgaa
      361 ggtgcggtaa aacgcactgg aggaccgaac ccacgtacgt tgaaaagtgc ggggatgagg
      421 tgtgggtagc ggagaaattc caaacgaact tggagatagc tggttctctc cgaaatagct
      481 ttagggctag cctcggaatt gagaatgatg gaggtagagc actgtttgga ctaggggccc
      541 atctcgggtt accgaattca gataaactcc gaatgccatt cattcatatc cgggagtcag
      601 actgtgagtg ataagatcca tagtcgaaag ggaaacagcc cagaccacca gctaaggtcc
      661 caaaatatat gttaagtgga aaaggatgtg gggttgcaca gacaactagg atgttggctt
      721 agaagcagcc accatttaaa gagtgcgtaa tagctcacta gtcgagtgac cctgcgccga
      781 aaatgtaccg gggctaaaca tattaccgaa gctgtggagt acacctttag gtgtattggt
      841 aggagagcgt tctaagggcg ttgaaggcag atcgtgagga ctgctggagc gcttagaagt
      901 gagaatgccg gtatgagtag cgaaagacag gtgagaatcc tgtccaccga atgactaagg
      961 tttcctgggg aaggctcgtc cgcccagggt tagtcgggac ctaagccgag gccgacaggc
     1021 gtaggcgatg gataacaggt tgatattcct gtacccgttg tttttgtttg agcaatggag
     1081 ggacgcagga ggctaaggaa tgcagacgat cggaaatgtc tgtccaagca gtaagtctga
     1141 agaggagtca aatgcttctt ttcttaagga caagctgtga tggggaggga aataatagta
     1201 ccgaagttcc tgatgtcaca ctgccgagaa aagcttctag tgagaaaaca gcggcccgta
     1261 ccgcaaaccg acacaggtag tcgaggagag aatcctaagg tgagcgagag aactctcgtt
     1321 aaggaactcg gcaaaatgac cccgtaactt cgggagaagg ggtgctgatc atacgatcag
     1381 ccgcagtgaa taggcccaag cgactgttta tcaaaaacac aggtctctgc aaaatcgtaa
     1441 gatgaagtat aggggctgac gcctgcccgg tgctggaagg ttaagaggag tgcttagcgc
     1501 aagcgaaggt acgaattgaa gccccagtaa acggcggccg taactataac ggtcctaagg
     1561 tagcgaaatt ccttgtcggg taagttccga cccgcacgaa aggcgtaacg atttgggcac
     1621 tgtctcaacg agagactcgg tgaaatttta gtacctgtga agatgcaggt tacccgcgac
     1681 aggacggaaa gaccccatgg agctttactg tagtttgata ttgagtgtct gtaccgcatg
     1741 tacaggatag gtaggagccg tagaaatcgg aacgctagtt tcgatggagg cgctggtggg
     1801 atactacccc tgcgttatgg ccactctaac ccgcaccact aatcgtggtg ggagacagtg
     1861 tcagatgggc agtttgactg gggcggtcgc ctcctaaaag gtaacggagg cgcccaaagg
     1921 ttccctcaga atggttggaa atcattcgaa gagtgtaaag gcagaaggga gcttgactgc
     1981 gagaccaaca agtcgagcag ggacgaaagt cgggcttagt gatccggtgg ttccgcatgg
     2041 aagggccatc gctcaacgga taaaagctac cctggggata acaggcttat ctcccccaag
     2101 agtccacatc gacggggagg tttggcacct cgatgtcggc tcgtcgcatc ctggggctgt
     2161 agtcggtccc aagggttggg ctgttcgccc attaaagcgg cacgcgagct gggttcagaa
     2221 cgtcgtgaga cagttcggtc cctatccgtc gcgggcgttg gaaatttgag aggagctgtc
     2281 cttagtacga gaggaccggg atggacttac cgctggtgta ccagttgttc tgccaagggc
     2341 attgctgggt agctatgtag ggaagggata aacgctgaaa gcatctaagt gtgaagccca
     2401 cctcaagatg agatttccca tttctttaag aaagtaagat ccctgagaga tgatcaggta
     2461 gataggtcag gagtggaagt acagtgatgt atggagcgga ctgatactaa tcgatcgagg
     2521 acttaaccaa taggttaaaa gaaaagaact cggaaaaaaa cttatgtgac ttcaaatcca
     2581 gttttgagtg aacaaaattt actcaataaa agataacacc acagtgtggt ggcgatagcg
     2641 agaaggatac acctgtaacc atgccgaaca cagaagttaa gcttcttagc gccgattgta
     2701 gtgaggggtt gccccttgtg
//
LOCUS       DAGCWU010000211         2698 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.217, whole genome shotgun sequence.
ACCESSION   DAGCWU010000211 DAGCWU010000000
VERSION     DAGCWU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.217"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1481
                     /locus_tag="MBZ71_002623"
     CDS             <1..1481
                     /locus_tag="MBZ71_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430517.1"
                     /translation="FYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKY
                     FLFFTVFLVLCWLPYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVH
                     DLPTDTINHGVFLFSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPI
                     YALNMQKDTLFSIACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVV
                     TGTGLICGLIILKQKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQ
                     ISRTIVMDGDLSKKDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFL
                     SLWARNLPSNIKIYTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLT
                     GLSGMKKIIDARDFFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATP
                     VAFSLRYVFVLALGLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            1468..2670
                     /locus_tag="MBZ71_002624"
     CDS             1468..2670
                     /locus_tag="MBZ71_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430518.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT          762 a          478 c          461 g          997 t
ORIGIN      
        1 ttttttactt tcttgtttat ttctccattt ttgtgtattc ttttttattt ttacaatcga
       61 tttatttatc aatgccaaaa ctggaaacat tttttactgc tcaaaacaca caggctttct
      121 cttcaagaaa atatttttta ttctttactg tctttttagt tctgtgttgg cttccttatc
      181 tgttggcatt gtatcctgga gtggttttgc ctgattcgtt aagttcggtc gcccaatcta
      241 tgggtgatgt tcctataagc aatcatcatc cagtcttatt caccttactg gtcaagctat
      301 ttgttcatga tttaccaact gatacgatca atcacggtgt atttttattt tcgatttttc
      361 aaacattgat aacagcttca gcgataagtt attctctttg ctggctgtta aaaagaaaca
      421 tccatttttt tccggtactc attagtttat gctattttgc atttgcaccg gtttttccaa
      481 tctacgcctt gaacatgcaa aaagatactt tgttctctat cgcttgcttt ttattttcct
      541 tagcggtttt tgactttatt tttcaacgag aaaagccaac gattctcaat cagatttacg
      601 taatcatcct tgctttactg accacctatc taagaaataa tgggatttat gtagtaaccg
      661 gcacaggttt gatttgcggc ttgattattt tgaaacaaaa aaggtatcaa gcattctttc
      721 aagtggtgct gggttataca gtgattgctg gactactcat ccacccgttg atggctcgtt
      781 atgctattcc tacagaaact gctgaacaat taggtgttcc tttgcagcaa atttctagaa
      841 ctattgttat ggatggtgac ttgtctaaga aagataaagc attcatgaat cagctactcc
      901 cgataaaaga ttatgaagcg tattctccta gtcttgcaga ctcaatcaag tggcataaaa
      961 attttaacaa tgagtttttg aatacacata aaaaagaatt tctatcacta tgggcaagaa
     1021 atttaccaag taatataaaa atctatacag atgcttatat tttagaaacg ttcgggttct
     1081 gggtacctgg tacaaaaaat agttacggct ttttagatac tcgtgtaaac acgaataatt
     1141 atggtatgaa gcaagtagat ttgattgaac atcttactgg attgtcagga atgaaaaaaa
     1201 tcatcgatgc tcgtgatttc tttggttccg gcacattact gtggttagct atcggcagtg
     1261 cacttttagc aatctttaag aaaaaatatc ggtatttact cctttttctt cccggtatgc
     1321 ttgtttttgc tactattatg attgctactc ctgtggcttt tagtttacgg tatgtgtttg
     1381 ttttagcatt aggtctacct gtttatatcc tctctccttt tttactagat aaaagagaaa
     1441 aaatgaaaac agatatagga gatactcatg aaactattta aaaagatatt ttttgctttt
     1501 gttctcttga tcgctagcta ttgcgtacta ctgacaatta cttttttcat tccgcagcac
     1561 tctattgaat cgaatgcaga aaaatctcta cagatggttg aaaaagaagg aatgtatcct
     1621 tctatcaatc aaggaaatat gctaggcact cggttagata attttactga tcatttgatg
     1681 atacgaaaaa caaaagcaga tcctgaattg aactccttgg agaacgcgat gtctatggcg
     1741 gattatcccc gttattggca tggttatcag ctttttttac gacctttact gctagggatg
     1801 agtttgggga gtattcgaat gatttacgca gccattcttt ttctgctgat tggattgacg
     1861 agctactttt taaccaagtg tagcgacatc tatttaacga cagccttgtt gatttcgctt
     1921 gctataggaa atgctgcaac ctttttcttc agtatgcaat tttcaaatat ttggattttg
     1981 actttattgt ctgtattact atttttaaca aaaccacaac tctccaacac caaccaacaa
     2041 ttattcttgt atttctttgt cattggttct ttagctaact tttttgactt gttgacaaca
     2101 ccggtcatct cttgggggat acctgtgatc attatttatt atgtaacaaa taagtattta
     2161 atggataaac gcagttcatt gagcaaacaa gtaggcagtt ttgtttttac cggtatcttt
     2221 tggggattag gctatggttt gacatgggtg gctaaatgga tcctatcatc gatcatattg
     2281 aacaaaaaca ttgtaaaaga tgcaatcaac caaatcctct ttcgaacgga aggaaacgac
     2341 aaatacccgt tgcatcgatt agagatgctg aaaagcaata ttagattgat gtatccaaaa
     2401 gtagcaatat tattgctatt gattacttgt cttgtttttc tctattttgc ttatcgtaaa
     2461 cgaaatagct ggcgtccgtt ccaacttcta gttttgtttg ttttagctgc tgtaacgccg
     2521 tatatttggt acaatatttt agctaatcac tcacagattc attattggtt tacgtatcgt
     2581 acgcaaatca tcacaagttt tgctatactt agtgctttcg cctttttgat ccctcctgag
     2641 aaatcaaaag acgaactaaa ttacttttaa gaataccgat gtactaattc atttttaa
//
LOCUS       DAGCWU010000212         2647 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.218, whole genome shotgun sequence.
ACCESSION   DAGCWU010000212 DAGCWU010000000
VERSION     DAGCWU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.218"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(1273..1938)
                     /locus_tag="MBZ71_002625"
     CDS             complement(1273..1938)
                     /locus_tag="MBZ71_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4430519.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..>2647
                     /locus_tag="MBZ71_002626"
     CDS             2064..>2647
                     /locus_tag="MBZ71_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBT4430520.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDV"
BASE COUNT          854 a          422 c          475 g          896 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtata
//
LOCUS       DAGCWU010000213         2637 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.219, whole genome shotgun sequence.
ACCESSION   DAGCWU010000213 DAGCWU010000000
VERSION     DAGCWU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.219"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            170..514
                     /locus_tag="MBZ71_002627"
     CDS             170..514
                     /locus_tag="MBZ71_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBT4430521.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="MBZ71_002628"
     CDS             515..934
                     /locus_tag="MBZ71_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBT4430522.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="MBZ71_002629"
     CDS             complement(1026..1376)
                     /locus_tag="MBZ71_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430523.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2562)
                     /locus_tag="MBZ71_002630"
     CDS             complement(1342..2562)
                     /locus_tag="MBZ71_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBT4430524.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT          982 a          478 c          516 g          661 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttg
//
LOCUS       DAGCWU010000214         2622 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.220, whole genome shotgun sequence.
ACCESSION   DAGCWU010000214 DAGCWU010000000
VERSION     DAGCWU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.220"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..321)
                     /locus_tag="MBZ71_002631"
     CDS             complement(<1..321)
                     /locus_tag="MBZ71_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430525.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQ"
     gene            complement(333..533)
                     /locus_tag="MBZ71_002632"
     CDS             complement(333..533)
                     /locus_tag="MBZ71_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4430526.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            656..1258
                     /locus_tag="MBZ71_002633"
     CDS             656..1258
                     /locus_tag="MBZ71_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4430527.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1313..2479
                     /locus_tag="MBZ71_002634"
     CDS             1313..2479
                     /locus_tag="MBZ71_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4430528.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT          938 a          445 c          388 g          851 t
ORIGIN      
        1 ttgatgactc aatatccatt ttttgcattc ttcttttgaa aacaactcca ttttttctaa
       61 taaaataact ggcaatccca ttcttttaaa tttcaaaaaa gttgtatatg atacattcaa
      121 atatttagcc atctcttttc cttttagaaa tggtctttct aatccggtat caattcttgc
      181 ttgatcaata gcctcttttg tcatttcaaa aacataattt ttcaattgat tcgtttgttg
      241 tttatctaac attactgtaa ttcctgcatc cattattttt ctcctctcat tttctatttc
      301 ttcaatcagc caactcgcca agttgtattt atttagattt tcaaatcatt aagtaaccaa
      361 tttaccaact tttgataagt agatgctcta atactttctt tgctcccatt ttcgattttg
      421 acatatgttt tccgatttat acctatcaat tttgctgcat cgtttttagt aagtataaga
      481 tcggctctat gtcttcttaa gcttctaatg aattgatcat taagagttat cattcagcgc
      541 ctcctttcaa attattcact ttgtggtttt tatcttaata agcacatttt aaaccacaaa
      601 acgagtaata tcaattaaaa tattactcaa tttgtggttt tcatgctata atagtttgga
      661 atttactata gggggcgtta ctttgaatag aataaaattt tatattgatt ccagtaaaaa
      721 gaccattaaa caaattagta cagaaactgg tattaactat acaactctta cgaactatta
      781 ccgtggtgag cgaaatccta gaaatactga aacatggaat atccttgctg attatttcca
      841 tgttcctgta agttatttga tgggattaga cgaaacaaat tccatagaac aacctaatct
      901 tgatagaata aaaactgctc gtttagaaaa aaaggacaaa atagtcaaga atattgccag
      961 tcttgactca gattatttta ctgaattatc taatctatac ttttccatga cttctttact
     1021 tgaagtcaaa cttcaagaag atgactccga ctttcttttt ttaaaaagta ttctagagga
     1081 ctcctttgaa tacacgattc tatttgccaa acaaaagaag tttattaata aagctttaaa
     1141 cttaaatatc gatgaaagaa aagagttaga atctgagtta tttggcaact taacccaagt
     1201 acaggttaac aatctaaaaa gagagataac agctcgatta atatcaaaac ttcaataaaa
     1261 ctaattattc aatcagccaa ctcgccaagt tatcagattg gagagattag ttatgtcaat
     1321 acaaaagtac acaacaaaaa gcggtgcacg atatagtgtc cgcttgtatg ttcgtaaaga
     1381 tgaaaatgga aagcaagaat actatgtaaa aaaagggttt aaaactgaaa aagaagccaa
     1441 gttacacgaa gcaagaaaaa aggtcgagat tgaagatacc ggctatcata agccttctaa
     1501 tgacttgttt ataacagtgt atgaagaatg gttgccttct tataggaatc gtgtacaaga
     1561 aacgacatat caacgtacaa aagacctttt ccgcttgcat atactaccaa aattcgggaa
     1621 taagtccatt tctaagatta ctcctgttca ctgtcagaat gcagtcaatg aatgggcttt
     1681 aaaattcaag aatatcaaac agcttaagtc ttacagctct caaatatttg agtatgccat
     1741 ttttgcagag ctattacacc gaaatcctat ggcaaataca aatctaccaa agcgggaaaa
     1801 gaaagaaaat gaaaattact tttcattaga agaactaaaa gctttttggg atattctcaa
     1861 taaagaagag cctttaaaac atattttaat cttccagctt ttgattacta ctggtatacg
     1921 taaaggagag ctagctgctc ttcattggtc tgacatttat tttgaagaac agctaatgta
     1981 tgttagaaag agctatgcaa ctattcgaaa tgaagagaag aatgcaagaa gaaaaactgt
     2041 caggattcaa aaaaatacaa aaaatatatc ttccgaacga atattaccaa ttgatgcaca
     2101 aactattgaa atgttaaaaa tctggaaaaa agaacaagct cgagagcttt tgcagtttgg
     2161 cataaataca aatacaaaaa atcaacttat ctttacctat gtaaatgcag acggtgacat
     2221 taatcaacct ttgcatgcgg actattcaaa taatatattg aaacgtttag agaagaaata
     2281 taaatttaag cacgttacta ttcatggatt ccggcacact cacgctactc ttctactaga
     2341 aggtggagca agtatcaaag aaacacaaga tcgattgggt cataaaaatg ctgaaactac
     2401 ccttaacact tattctcatg ttactgaaaa agctcaacga aatgcgttag aaaaattcat
     2461 tgcttacact ggtttataat attgtcatta cctttctcat taccagaacg aaaaaaagac
     2521 accttctcgc aatgagaaag tgtctgaaat cgttggtata acaacgatta acgttttgaa
     2581 aactgtgaag ctttacgtgc tttcttaaga cctggttttt tc
//
LOCUS       DAGCWU010000215         2569 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.222, whole genome shotgun sequence.
ACCESSION   DAGCWU010000215 DAGCWU010000000
VERSION     DAGCWU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.222"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            58..309
                     /locus_tag="MBZ71_002635"
     CDS             58..309
                     /locus_tag="MBZ71_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBT4430529.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            411..2408
                     /gene="tkt"
                     /locus_tag="MBZ71_002636"
     CDS             411..2408
                     /gene="tkt"
                     /locus_tag="MBZ71_002636"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBT4430530.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
BASE COUNT          853 a          467 c          581 g          668 t
ORIGIN      
        1 agttgttttt ttttcagttt ttatataata tgaaggagag taaaaggagg gctgagcatg
       61 ctgtcacctg aaaaaataga gcggatcaat gaacttgcac gtaaaaagaa ggaaaatggt
      121 ctctcaaaag ccgaagaaca agaacaaatc aacttgcgca aagagtattt ggaagctttt
      181 cgttccggta tgcgtcatca tatcgaagga atgaaagtcg tcgatcctga gggcaatgac
      241 gtgaccccgg aaaaattaaa agaaatccaa aagaaaaaag gcttacacaa ccgaaatgac
      301 aacttttagt aaaaaatact gaaaaagatt gctttttcgt gtgacatctt atagaatgaa
      361 agtgtaagaa ataaaggtaa aaaaaaccta aattaatagg aggggttatt ttgtttgaca
      421 aaattgacca acttggcgtt aacacaattc gtacattaag tattgaagca gtccaaaaag
      481 caaactcagg acatcctgga ttgccgatgg gcgcagcacc tatggcatat gcgttatgga
      541 caaaacacct gaaagtaaat ccaacgacat caaaaaactg ggctgaccgc gaccgtttcg
      601 ttctttcggc aggacatggg tcagcaatgt tgtatagctt gcttcattta gctggctatc
      661 aagtgacaat cgatgatttg aaacaattcc gtcaatggga ttctaaaaca ccaggacatc
      721 ctgaagtaca tcatacagat ggtgtagaag ccactacagg gccattagga caagggattg
      781 caatggctgt cggtatggca atggcagaag cgcaccttgc agcaacgtat aacaaagaaa
      841 actttaatgt gatggatcac tatacgtatg caatatgcgg tgacggagac ttgatggaag
      901 gggtatctca agaagcaagt tcgatggcag gtcatatgaa actagggaaa ttgatcgttt
      961 tatacgattc aaacgatatt tcactagacg gtccaacttc aaaagctttc acagaaaacg
     1021 ttggtgctcg ctatgaagca tatggctggc aacatatctt agtgaaagac ggaaatgacc
     1081 tagaagcaat ctctaaagcg attgaagaag caaaagctga aacagacaaa ccaacattga
     1141 tcgaagtaaa aacagtcatc ggctatggtg ctcctaaaga aggaacatct gctgttcatg
     1201 gtgctcctct tggtgaagat ggcatcaaga tggctaaaga agtttatggt tgggagtatc
     1261 cagatttcac tgttccagaa gaagtagctg cccgcttcca tcaaacgatg atagaagaag
     1321 gacaaaaagc tgaagatgca tggaatgaaa tgtttgccaa ttataaaaaa gcttatcctg
     1381 aattagcaca acaatttgaa gatgcttttg atggcaaact accagaaaac tgggatgcag
     1441 aattgccaac ttatgaagtt ggaagcagtc aagcaagccg tgtttcaagt aaagaagtca
     1501 tccaagaatt atcgaaagct ataccaagct tctggggtgg ttcagcggac ttgtcaggtt
     1561 ccaataatac aatggtggct gcagataaag atttcactcc tgaacattat gaaggacgca
     1621 acatctggtt cggtgtccgc gaatttgcaa tggcttcggc tatgaatggt atccaacttc
     1681 atggcggaac tcgtatctac ggtggaacat tctttgtctt tgtcgattat ctaagaccag
     1741 cagttcgttt agcagcaatc caacatactc ctgttgttta cgtcttaaca catgactcgg
     1801 ttgctgtcgg agaagacgga ccaacgcacg aaccgattga acaactagca tctgttcgct
     1861 gcatgcctgg tgttcaagtg attcgtccgg cagacggaaa tgaaacacgt gctgcttgga
     1921 aagtagcgat ggagacaaca gatgcgccta ctatccttgt attgagtcgt caaaaccttc
     1981 cagtattgcc ttctacaaaa gaagtagcag atgaaatggt gaaaaaaggc gcgtatgttc
     2041 tttctccatc acaaggagaa acaccagaag gtattttgat tgcaacaggt tctgaagttg
     2101 atttggctgt gaaagctcaa aaagaattgg ctgaaaaagg caaagatgtt tctgttgttt
     2161 ctatgccaag ctttgacctc tttgaaaaac aatcttctga atataaagaa agtgtacttc
     2221 ctaaatcagt gaagaaacgt gtggctatcg aagctgctgc atcatttgga tgggaacgct
     2281 acgtaggaac agagggcgca acgattacaa tcgatcattt cggtgcatct gcccctggca
     2341 ctaaaatttt agaagaattt ggatttacag tagaaaatgt cgtgaatacg tacaatcaac
     2401 tatcatagat ccaagcaatt gtttgactga taagaacagc tgtctgcaag ttggagacag
     2461 ctgttcttta tgtgttagaa gaataaatca atattacttt tcgttttcgg ctatgttaca
     2521 tttaaatgac aaaacaaaga attcttcgga ttagtttgtg tttcttgag
//
LOCUS       DAGCWU010000216         2556 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.223, whole genome shotgun sequence.
ACCESSION   DAGCWU010000216 DAGCWU010000000
VERSION     DAGCWU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.223"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            144..494
                     /locus_tag="MBZ71_002637"
     CDS             144..494
                     /locus_tag="MBZ71_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4430531.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="MBZ71_002638"
     CDS             908..1726
                     /locus_tag="MBZ71_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBT4430532.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="MBZ71_002639"
     CDS             complement(1799..2158)
                     /locus_tag="MBZ71_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4430533.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          844 a          366 c          468 g          878 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttatatatct atttctctta ggagtatgtt tagatgtttg aataatagtt
     2461 gcccaacttt aaaggttatt cttataatat tttacttcat tttccaatag aaaattcaaa
     2521 ggattactgg tttttctaaa atagtttagt ttaaat
//
LOCUS       DAGCWU010000217         2523 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.224, whole genome shotgun sequence.
ACCESSION   DAGCWU010000217 DAGCWU010000000
VERSION     DAGCWU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.224"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            213..>2523
                     /gene="esp"
                     /locus_tag="MBZ71_002640"
     CDS             213..>2523
                     /gene="esp"
                     /locus_tag="MBZ71_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBT4430534.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHK"
BASE COUNT          915 a          381 c          505 g          722 t
ORIGIN      
        1 tttatatgtt ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag
       61 aaatgtactt tttttgttgt aatgtgcatg aaattcttta agtaaactag ttaacataag
      121 tttagtaaga ttcctattct gtgaataata cgtatcagat ttatctttta ttcttactaa
      181 gaaaataact attaagggag ttgatttgaa taatggttag caagaataat aagagagtat
      241 ttctagaaaa aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg
      301 ttgcatctgt tttggttggt gttggtcttg tcttgggaac aactgaacta gttcaagcac
      361 aagatgaaat ttctccaagc accccacttg aaacagcgat aagctccgta caagtaggtg
      421 acaaagtagc ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact
      481 tttcggattt acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa
      541 ttggatttga agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc
      601 gtcttcaatt agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga
      661 aaggaattgg atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta
      721 caaatatttg gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggctgaaa
      781 caactgatac acaaactgct cctaatgggg taattacgtt tgaaaagagc ttagatgaaa
      841 tttttaaaga gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact
      901 tggtaagtca tcaagacgat gataaaattg taccaggtat tgatagtacg ggctactttt
      961 taacagattc agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt
     1021 ttaaacatgg atcagtaagt gcaaaatatg aggaacctaa tactcaaaca aaagatggtt
     1081 caggatccac aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata
     1141 atttctcata ttcagcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg
     1201 aacgattagt tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa
     1261 catatgatgt ttcttttgaa tcagggaaaa aagtggctga tttagcaatt gaacggcgca
     1321 aagatcatga aaattatggt gtaggttcaa ttactgataa tgatcttacc aaacttattg
     1381 attttgctaa tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc
     1441 cattagatga aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat
     1501 ttggtgaaga tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga
     1561 acacttatgg aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg
     1621 ataaagaaga gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat
     1681 atgatgttga cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc
     1741 acaaagccca acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa
     1801 atgaaaaaga tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt
     1861 caagtaaatt aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata
     1921 aaccagaaga aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg
     1981 tggtagttgg atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg
     2041 aagatgcaga gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga
     2101 aaggtattgt aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg
     2161 taaaagcttc tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag
     2221 aaacgacagt tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag
     2281 atgccttttc aattttagat gtctataata aggacttcgg taatgcaagt gttgatgcaa
     2341 atactggtat tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg
     2401 gaacaattcc tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag
     2461 taactgtaag taaagatatt tatgaaaatc caggagaaaa cattcctgca ggctaccaca
     2521 aag
//
LOCUS       DAGCWU010000218         2492 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.225, whole genome shotgun sequence.
ACCESSION   DAGCWU010000218 DAGCWU010000000
VERSION     DAGCWU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.225"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(61..354)
                     /gene="rpmA"
                     /locus_tag="MBZ71_002641"
     CDS             complement(61..354)
                     /gene="rpmA"
                     /locus_tag="MBZ71_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBT4430535.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(367..711)
                     /locus_tag="MBZ71_002642"
     CDS             complement(367..711)
                     /locus_tag="MBZ71_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBT4430536.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(738..1046)
                     /gene="rplU"
                     /locus_tag="MBZ71_002643"
     CDS             complement(738..1046)
                     /gene="rplU"
                     /locus_tag="MBZ71_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBT4430537.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(1066..1142)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1328..1969
                     /locus_tag="MBZ71_002644"
     CDS             1328..1969
                     /locus_tag="MBZ71_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBT4430538.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            2131..>2492
                     /locus_tag="MBZ71_002645"
     CDS             2131..>2492
                     /locus_tag="MBZ71_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBT4430539.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIA"
BASE COUNT          771 a          523 c          449 g          749 t
ORIGIN      
        1 tataaacaaa aaaggctgtg acaaaagtct tgtcacaacc tctttaatca taagattcaa
       61 ttaagcttct tgagctactg gataaacaga cacttgtttt ttgttacggc ctttacgttc
      121 gaaacgtact acgccatcaa cttttgcaaa taaagtgtcg tctccaccga tacccacgtt
      181 tacacctgga tagatttttg taccgcgttg acggtaaaga attgatccac ctgtaactgt
      241 ttgtccatca gcacgtttag cacctagacg ttttgattct gagtcacggc cgttagatgt
      301 agaaccgcca ccttttttgt gggcaaataa ttgtagattc atagataaca acatggtctg
      361 cacctcctat ttatcattaa ttgttttaat ttggatatat tctgagtttt cagcttctat
      421 cgattgtaat cctaataaaa gattttctaa tagaatctca gcaatattgt tttgttcttg
      481 tgtcattcct gaagtgactt cggtataaag ataaccgcct tcttcttcat tcatttcaac
      541 aatcggctca aaaccggcta atgaggcaat cccgttgaca gtgctgatcg ctaacgcaga
      601 aacacccgca caaacgatat cacttccata aggaccagca tcagcatgcc cggtcaaagt
      661 gaatgaaacg atctgacctg cgtcatttcg cttaaatgtt cctttgatca tatataggcc
      721 ttctttctca aacgctatta agcgttgatc gcgtcgataa ccacttttgt atatggttga
      781 cgatgacctt gtttacggtg tgaatgtttt ttaggtttgt atttgaaagt aacgactttc
      841 ttttgtttgc cgtgtttttc aacagtacct tcaacagttg cacctgcaac agttggagcg
      901 cctacttttg tagattctcc gcctactaag ataacttcgt caaaaacaac tttttctcct
      961 gcttcaacgt caagtttttc aacatagatt gcttgtccca cttcaacttt tacttgttta
     1021 ccaccagttt taataattgc gtacatgcta taaagcacct ccttctgata tacttagact
     1081 cgccatccga agtgacagca tatgctgtgc ttattacttg ttctgagcgg ttgtagctgt
     1141 ggagtccaca attacaacag tactagctta ccagaacagc aaccgttagt caactaaatt
     1201 taaaatcaaa aagtaaaaca aaaattcaag caatacttct gcttttctat cctcacagtt
     1261 ctaccaaata aaatagtcgc attgtgtttc ttttattctt ataataaaag aaaaaggagg
     1321 agcataaatg gaatacccaa aagaactgat acaagaagct tctttaagaa tgaacggccg
     1381 tcctgtagaa ggatttatcg ctgggcaagg acctcttcat cctaaactga tgctcgtggg
     1441 agaagcccca gggaaaacag agattgatac gcatgttcct tttagcggtc aagctggaaa
     1501 agaattgatg caggcacttt cttcaactgg cttaacacga gaagaggtgt atatcaccag
     1561 tgctgtaagg agccgcccgt atcgcgtgac tcatcggatc aataaacgga cacaacagtc
     1621 agaaactgtt tatccaaatc ggacacctac tcgttccgaa gtatttgcgc atgcaccaat
     1681 attggattac gaactgatga aggtccagcc aaaattaatt gcgacacttg gaaacattgg
     1741 tcttcaacgt ttacttggca aagaatatac ggttaccaaa aatcatggtc aactctatac
     1801 agggcctgtc ttacaattaa cagaacaaaa ggatgcctac gaatggtcag aaaaaaacta
     1861 tcgaattctt ccactttttc atccagcagc tattttctac aatcgctccc ttgtacctgt
     1921 tatccatcaa gactggcaag tactagcttc acttttacag caatattaaa aagaacagac
     1981 aagaccataa aatcggctct ataatttatg ggactgatga atcagaattg attcttcagc
     2041 cccattttta ttttaggtat taaaaaacat atcgttctct agtatgatta aatcacgaca
     2101 aaataaacaa ctgagaggac gatatgctat atgaatgatt ctatcaaaaa aatgctgcga
     2161 ttaatagaga aagatttgat gattacagag gtctcttacg agacctttca gaagaaaaag
     2221 acgttgatcg tcgatgctgt tttctcacct gctccccata cctgtagaaa ttgtggttct
     2281 actgtggtag atggaaacgg gaaagtaatt gtagtgaaaa atgggaagaa ggaaacgatt
     2341 gtccgctttg aacaatacaa tcacatgcct ttggttatgc gcttaaaaaa gcagcgctat
     2401 acctgtaaaa actgtcgaac ccattggacg gctcaaagtt attttgttca acccagacat
     2461 tcaatcgcga atcatgtcag atataaaatt gc
//
LOCUS       DAGCWU010000219         2488 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.226, whole genome shotgun sequence.
ACCESSION   DAGCWU010000219 DAGCWU010000000
VERSION     DAGCWU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.226"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            2..1147
                     /locus_tag="MBZ71_002646"
     CDS             2..1147
                     /locus_tag="MBZ71_002646"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBT4430540.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1318..1776
                     /locus_tag="MBZ71_002647"
     CDS             1318..1776
                     /locus_tag="MBZ71_002647"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBT4430541.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1935..2405
                     /locus_tag="MBZ71_002648"
     CDS             1935..2405
                     /locus_tag="MBZ71_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBT4430542.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          826 a          470 c          469 g          723 t
ORIGIN      
        1 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
       61 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      121 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      181 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      241 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      301 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      361 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      421 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
      481 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
      541 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
      601 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
      661 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
      721 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
      781 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
      841 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
      901 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
      961 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1021 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1081 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1141 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1201 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1261 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1321 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1381 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1441 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     1501 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     1561 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     1621 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     1681 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     1741 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     1801 aagcaatata gctatcttat cttcgtattt tattaaatat ttattgtctg aaaagagttc
     1861 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
     1921 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
     1981 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
     2041 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
     2101 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
     2161 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
     2221 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
     2281 tcactaaagc aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa
     2341 tcgaacgatt tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc
     2401 tttaacttaa taaaaataaa aaaagatgaa ctatttctag ttcatctttt tgcctttgcg
     2461 tggcgacgtc ctactctcac aaggggca
//
LOCUS       DAGCWU010000220         2428 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.227, whole genome shotgun sequence.
ACCESSION   DAGCWU010000220 DAGCWU010000000
VERSION     DAGCWU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.227"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            128..2281
                     /locus_tag="MBZ71_002649"
     CDS             128..2281
                     /locus_tag="MBZ71_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBT4430543.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVLVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          831 a          425 c          537 g          635 t
ORIGIN      
        1 tgaaacaaaa agttgatttt atcttgaaat taggtaaaga atttcttgct ccgcccattt
       61 tttctttttt attaagaagt ttttgggtac aatgaagcat gaatgtaaat aggcggaagg
      121 ataggaaatg atgaaagatt ttatgaaaaa aatattaaat acttcttggt tagaaaaaat
      181 aagaaaaggt gcacaagaac ctaagaacaa aacgtttcgc cggtcccatg ttccttttcg
      241 cttaaatttt ctttttttca ttatatttac gttatttgtt gcgttgattg ctagattggg
      301 ttatttgcag atcgtgaacg gcgaagagat ggaggcgcga gtgaagtcta cttctacgat
      361 tacggtccaa gaaagtactc ctcgcggaat gatctacgac tccacaggaa aagctttagt
      421 tacgaataaa gcgaatcagg ccatcacata tacgagagga acacaagtta cggctaaaga
      481 tttgcttgaa atcgcaacga agctgaactc gctgatcgat gttctggtag atgaaaatct
      541 gacagataga gataaaaaag atttttggct ggctgatccc aaacatttga aagaagccac
      601 aaaaagactt tcggcaaaag aaaagcaatt agggaacagc gaacaatata gtgcgatggt
      661 caataaagtc acagctgatg agattaattt aaacgaggag caattaaaaa ttgctaccat
      721 ctttaaacgg atgaatagtg catatgaatt aaacactgtc tatatcaaaa actctggtgt
      781 tactgatcaa gagttagctg ttgtggcaga acgcgcttct gaacttccag gagtttcgac
      841 tggcacagac tggacacgag aatataatgc agcaagttca ctgaaaagta ttcttggcag
      901 tgtgacgact gaaaagcaag gacttccagc agatgaagca gagaaatacc tagcaaaagg
      961 ttattctcgg aatgaccgtg taggacaaag ttatttggaa aaacaatatg aagatgtcct
     1021 gcaaggaacc aaaacccaat atgaagtttc ccttgataat gaaggaaatg tttcgaatca
     1081 aaaagaaatc ttctctgggg aaaaaggttc taatttaatg ttatctatga atgcagaatt
     1141 ccaatcaaaa gtagaagaaa ttttgaaaag gaattatcaa acattgatca acaacggcaa
     1201 agcacagtat tcgcctggtg cttatgcagt ggctatgaat cctcagacag gagaagtgct
     1261 agcaatgact ggtttcagtc atgaacaagg ttccaaagaa atcaccgaga atgctttagg
     1321 cacgattact agcgcctttg atccagggtc tgtagtcaag gctggtactt tgacagcagg
     1381 ctgggcaagt aacgcgatca gcggaaatca agttttgatt gatgagccga ttcgtctgca
     1441 aggtgcttca gaaaaaagct ctgtctttaa tcgaagcgga caagttgctc ttgatgcagt
     1501 caaagcatcg gaattgtctt ctaatacgta tatgatcaaa gtggcgttaa agatgttagg
     1561 actagactat acacctggaa tgggactacc tagtttagat gaagaagcta aagcttatca
     1621 gcaattgaga gactctttca aagagtttgg tcttggtaca acgactggga tcgatttgcc
     1681 gaatgaatct cctgggatct cacgttctgt tgattatatg aagaagttca atgcggataa
     1741 tggcaaagaa tggtatgctc caggtaactt taccgatttg gcatttggac agtttgacac
     1801 atatacacca atccaattag ctcaatatgc ttcaacagtc gctaatggcg ggaaacgagt
     1861 acagccgcac ctagtcaaag cgatctatgg caatgacgaa aatggaaact tgggtgaagt
     1921 gaagaaagaa attggcacaa cagtagaaaa tacggtcaat atttctgcag aaaatatggc
     1981 gatcctccgt gaaggattcc accaagttgt ccatggtacc gatccttaca caacagccaa
     2041 accattagcc agtgcgaaaa tggacctttc cgcaaaaaca gggactgcag aaacggttgc
     2101 ggaaggtcat ccagatattg cgacagtcaa tagtaatatc gtggcatacg gaccaacaga
     2161 caatccggaa attgccatca gtgttgtctt gccgaactta ttagacgaac aagaccacat
     2221 aaacttgacg attgctaaag aaataatgga cgcgtattat gatatgttca tggcaaaata
     2281 aaaaagaggt cgtgaaaaag ccgcttagtt tcgagtatga agaaaaattt tgtgaaaata
     2341 gcttctcaca tttttgctta aatttgactt cataccgaga aactggcttt tgaacaccgt
     2401 ttactcggca aaaaaggttg tgacaaaa
//
LOCUS       DAGCWU010000221         2423 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.228, whole genome shotgun sequence.
ACCESSION   DAGCWU010000221 DAGCWU010000000
VERSION     DAGCWU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.228"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            3..476
                     /locus_tag="MBZ71_002650"
     CDS             3..476
                     /locus_tag="MBZ71_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBT4430544.1"
                     /translation="MAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETKSLF
                     LKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEK
                     QIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            513..1523
                     /locus_tag="MBZ71_002651"
     CDS             513..1523
                     /locus_tag="MBZ71_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBT4430545.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1638..2351)
                     /locus_tag="MBZ71_002652"
     CDS             complement(1638..2351)
                     /locus_tag="MBZ71_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430546.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          816 a          402 c          495 g          710 t
ORIGIN      
        1 acttggcaaa gaaattctta cacgaaaata tatattttga gttgtatcat catcgagcca
       61 tttatacaaa tgaagatgct ttggtaataa agaaagaaca aggatttaca ggtacagaga
      121 ccaaaagctt attcttaaaa gataaaaaag ataataacta cgtatatctg acttttacta
      181 cgaaacaaac ggatttcaaa gcattaggga agatcgtcgg taaaaggctt tctattgttt
      241 catcagaacg catggaagaa caaacgggac aaaagcaggg cgctgtttct ccgtttggtt
      301 atgaaaaaca gattccgatt attattgatg aagagctctt atcaaatgaa aaacttgttt
      361 ttgcaccagg tagaccagat caaacgatgg tcatccaaac ggttgactta aacaaaatca
      421 tccagctact tgagatgccg atatttttga tgccggcaaa cgaaaaaaac aattaaccaa
      481 gtaaaatagt gcatacttgg aggtgaagca acatgataag ttatccatta ttagaaagaa
      541 atgcaacgat tggggttact gcaccatcct caggcgtatc agaggaacac cattcgttac
      601 tcgaatcagc tttaaagaga atggaagaaa aatctttcca agtagttact ggaaagacgc
      661 tttggacgca agataaggaa aaatcaggtc cagcaagaaa acgagcagat gaattcaatc
      721 aaatgatgca agatgaaaat atccaaatga tctttccgcc ttggggagga gaattattga
      781 ttgagattct cgattttgtc gattttgatg ctctacaacc aaaatggata gtaggttatt
      841 cagatattag tacgttgcta cttgctgtca cattaaaaac agggatagcc actgcacatg
      901 gaaccaacct tattgattta agaggagagt acttggatga aacgacagca atgtgggaaa
      961 gtgtactgaa aacaagagaa ggagaatcga ttctacagca accttctgaa aagtatcaaa
     1021 aagagtggcc ccatgagcat tcatcatctt ctgttttcca tctcactgaa ccaacaaatt
     1081 ggcaatcagt cagcggagat gcagtaagga tgaagggacg attattaggt ggctgtattg
     1141 atacgatcag tcatttaatc ggtacccctt atggagatgt ccaatctttt aaaagaaagt
     1201 ataatgatgg aaagccgatc ttgtggtttc tagaaaattg tgagctatcg tcagttgatt
     1261 taagaagatc acttgttcag atgaaactgg caggatggtt tgatggcagc tcggggatca
     1321 tgttcggtag aagtgcagca gaggatttgt tagattataa aatgaaggat gtatatgaag
     1381 atctgtcaaa ggaattaaaa ctaccgatct tgtatgatat tgattgtggt catgtaccgc
     1441 cacaactgac attgatcaat ggtgcctatg cagaagtagc ggtagaagat agaaaagcga
     1501 ctgttttgca aacgtttatt taaaaaattt tttcctgcct tggagtttat caaagaccaa
     1561 caaatctaaa tagaaaagaa aatgaaagaa gctgacagta ttttgccagc ttctttttgt
     1621 atgatctatt agaaatttta attatccata actatcacat tgatcagctg tttcacatca
     1681 tttggattac ctgtaggaat agctactaca gatgcattat caataatttc ttgagttgtt
     1741 tctctaatga acgctggata agatgtttga tctgcaggca tttgccaatg tgaaggaact
     1801 ggataactag tgattgtccc gtcatggtta cttgaataca ctaaaactcc ctggtaactg
     1861 tatttcccag tgagcataac cacatctttc ggatagactg cactccctgc atcataaggg
     1921 gcgataggtg ttcctgcagg ataatgaata acatgtagtt cgaaatcacc cgatgtatct
     1981 ctaaaagtat tacctctaat tgctagccaa acacgtgcgt actcaatttg tagatcagaa
     2041 tatcctgcta acggatctat tgactgagaa gactgtgagg aagttgtgtc tattgttgaa
     2101 gaagaaatat ttgtctcaac tgtactacta gatgtagaag aagtattggg ctctgagact
     2161 ttactgcttg ttgaagatgt attcgtttca gtagaagctg atttctgagt acttgatttt
     2221 tcagctgaac tggtttgttt gattgaactt ttgtctgtgg tcgcagatgg atcactattt
     2281 tctttactag gagacgtaca tcctacaagg aataaagaga ccaacaataa aataaaacct
     2341 aactttttca tataaaatac ctcattattt ttttaatacc agtatagcat aatcctgaat
     2401 ttttgaatcc gtaaaacctt gtc
//
LOCUS       DAGCWU010000222         2412 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.229, whole genome shotgun sequence.
ACCESSION   DAGCWU010000222 DAGCWU010000000
VERSION     DAGCWU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.229"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            612..1424
                     /locus_tag="MBZ71_002653"
     CDS             612..1424
                     /locus_tag="MBZ71_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430547.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          841 a          335 c          372 g          864 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa taacttttat
     1681 attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag attagataga
     1741 tattataaat atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata
     1801 aaagaaggta tcaagagtca cggtgaattt attccgaaaa gtgaaaagaa caaatataaa
     1861 atttaataaa aatatagaac taaataggaa aacgtagacg taattttcta cgttttcctt
     1921 ttcatatata ttaaatatta cggaaattta tataaactaa tttagtgttt tgtttgtaat
     1981 tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatc agacattcta
     2041 tgctcatttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt atttgaaaat
     2101 tcttctttta gagtactttt attacgatac tagtatgata tatatctata gcaaacagtt
     2161 aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt tagatgaagc
     2221 gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct ttcaaattta
     2281 gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt atgtgacaaa
     2341 aagaactagt agaagaagga ataatggctt gataataaag tactacctat taaaggtagt
     2401 actttattta at
//
LOCUS       DAGCWU010000223         2401 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.230, whole genome shotgun sequence.
ACCESSION   DAGCWU010000223 DAGCWU010000000
VERSION     DAGCWU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.230"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(4..249)
                     /locus_tag="MBZ71_002654"
     CDS             complement(4..249)
                     /locus_tag="MBZ71_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430548.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="MBZ71_002655"
     CDS             complement(266..1117)
                     /locus_tag="MBZ71_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBT4430549.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="MBZ71_002656"
     CDS             1283..2287
                     /locus_tag="MBZ71_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBT4430550.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          771 a          404 c          485 g          741 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa
     2401 g
//
LOCUS       DAGCWU010000224         2358 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.231, whole genome shotgun sequence.
ACCESSION   DAGCWU010000224 DAGCWU010000000
VERSION     DAGCWU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.231"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(299..460)
                     /locus_tag="MBZ71_002657"
     CDS             complement(299..460)
                     /locus_tag="MBZ71_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430551.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
     gene            complement(457..1128)
                     /locus_tag="MBZ71_002658"
     CDS             complement(457..1128)
                     /locus_tag="MBZ71_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBT4430552.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            1651..2241
                     /locus_tag="MBZ71_002659"
     CDS             1651..2241
                     /locus_tag="MBZ71_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBT4430553.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT          760 a          396 c          362 g          840 t
ORIGIN      
        1 aaaaataccc caagtcttct tatgtaaaaa caccggttat tacttaaata tgacttaggt
       61 aaaacaatga gtgatccaat tataaaattt tatggcgaat aatttagtaa gcgctacata
      121 ttgaatgatt tagaaatttt gaaaaagtag ctagttcctt ttataccaga aaagtaaacc
      181 taataaaaaa cacaactaat ttttagctgt gttttttaag ttacaataat tatctattta
      241 atagtcttaa ttcattaaaa caaaaaactc aactttacaa ggatatggtt taaaaaattt
      301 atttatccaa cccaactagt tttcgctgct tacgatttgc ttcatctgct acgtacttca
      361 ttactgtttt aaattctttc gctgaggcgt tttctgataa atcagcgata cttttattat
      421 acttcttcac gatttgttgt acttgttctt tctcagtcat gtaattcctc ctccaactct
      481 tttgaagtat attgtattgg gtagtactaa ttgaatatta tctgctgcat aatgcacttc
      541 agaaatgtcg ttttgataat ccttaatcat tatgtgaagg actacttcag gatgtctatt
      601 tttgactttt tcaatattct ctctggactt aaaaaacgca ttgataaaca tttcttttgg
      661 cacatgacgt ccttcagcta gttcacgttt tcgagtgaaa tcccaggcaa ctttgggatc
      721 ttggtaaaca tagaaaatcg taacgcgaaa gttattttta agggctcgaa taatattttt
      781 ctctgcacta aggatagcaa aagtatcctc tagtatgaaa gaatagccat gttccagtac
      841 atattttaat gattgttcaa ctaaccaaga agaagctttc tgaaaaacac ttgagttctt
      901 tccattgtat tctggaaatt gtgctctaaa atagtccgca tcaatcacaa catggttgtc
      961 ataattttca gctaaaccta gtgcaacctc tgttttacct gctcctgggc ttccagccat
     1021 gaataccgca tctttttcat catcagggat ttttcccttg atgattgttt caataaattt
     1081 atctttattg accctagcgt aatctaaata cttgtcttta ggttccaaga ttgcaccttc
     1141 tttccttctt ctatattcat actaattata cgatatttat ataaaaaata taacggtcat
     1201 tttaaccata ttaacattta gtcacagtaa tagcagatat gctcgcaaaa tgagatatga
     1261 atgtacgcag aatccttgtt ttctacggca ttcgctgttt tttcattgga ctagttatat
     1321 atttctaggg ttgttttcat gtctgagtgc ccgatatgat ttatttttcg tttctataat
     1381 tactttttgc cttccagttt cttttaagat tgtactcaag caagtgggaa ttggttcttt
     1441 tgtcatccat gtatgcaaaa tgttataatg ctggtaaaca aaaatgatta cctgtcattt
     1501 ttatttagtt cgtatttaat taagttgact gtctgtatac tctgtctaaa acttaagaat
     1561 tgttgttttc gctgtcgcta tccaaattta atgaatgtct gtatgttaaa actgttgtcc
     1621 ttgtcataga tagtaaagga gagcttgaaa atgaaaactg cgattacgtt tctattagag
     1681 gaatatgccg attgggaagg tgcttatatt actagcacac taaaccaaaa agagggatgg
     1741 caaaacaaaa ctgcttctat ccaatcagag gtcacttcca ttggaggatt aaaaacaaaa
     1801 atcgattact taatcgaaca aatacctgaa aaagttgacc tttttattat gatcggtggc
     1861 aattcatggg acataaaaaa tcatcattta tatcaaataa tcgataaatt gttaaaaaca
     1921 gacgtaccag tagctgctat ttgtggggca gttgattatt tagctcagag cggattattg
     1981 caaggttaca aacacaccgg aaatgcgcaa tttccttggt tagaatcgga aaattatcag
     2041 aataaagaag attttataga acaacaagct gtgaaagatc gtaatttgat tactgcgaat
     2101 ggaacagctc ctatagaatt cacagacttg attcttcaag cagtccattt tgaaacagaa
     2161 gaaataagag aaaaaaagat tcgtctatac aaacttggat tctatgatta ctataaaaag
     2221 tacggagatc ctttctccta atcattctct tcaattaaat actccttatc aatagtatga
     2281 caagtaattt tactgatggt tatttgtcac actttttctg acatttatca aagaagatac
     2341 actctattta tattcata
//
LOCUS       DAGCWU010000225         2338 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.232, whole genome shotgun sequence.
ACCESSION   DAGCWU010000225 DAGCWU010000000
VERSION     DAGCWU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.232"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..951
                     /locus_tag="MBZ71_002660"
     CDS             <1..951
                     /locus_tag="MBZ71_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBT4430554.1"
                     /translation="AIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTIKYAKRK
                     KQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEKYVSAFN
                     IVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVD
                     NLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLEMLFEEI
                     DVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDSYGLVDV
                     FKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            948..>2338
                     /locus_tag="MBZ71_002661"
     CDS             948..>2338
                     /locus_tag="MBZ71_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBT4430555.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFP"
BASE COUNT          780 a          306 c          379 g          873 t
ORIGIN      
        1 gcgattataa ctgatttacc agattatatt aaaagaattg attccgattc ttttttagct
       61 gccttttatt tgtatacagc taagtattgg atagacgata gtcggaaaac gattaagtat
      121 gcaaaaagga aaaaacaatt ttattttcaa acatggcacg gtacgccttt aaaaaaaatt
      181 gaatttgatg caaaagataa gttacctaaa cgttacctaa gctatgcgaa aaaagatagt
      241 gagtcaatta catatttatt atctggtaat cgctatagtt ctgagaagta tgtttcagca
      301 ttcaatatcg ttccttctaa aatattagaa gtaggaactc ctagaaacga tgaattagcc
      361 tctagtaaag agcaagtgtt tgacttgaaa aagaaacaaa tcaatgtctt atttgctcca
      421 acttttcgaa ataatattga ggacaacgga attacgcaac tagaatggtt aggtgtggat
      481 aacttacgtg aattttttaa aaagcaacaa caagaattaa atttaatgac taaatttcat
      541 cctaatgttc atagtaaatt agcaactgat tctaaaagta tagaatatat gaaaaaacat
      601 caaattactt taattaatga tgggattcct cttgaaatgc tttttgaaga gatagatgtt
      661 ttaatcacag attattcatc tatttttttc gattttgcgc taacaaaaaa accaattatt
      721 ctttttaact atgatgaaaa agaatatagc ttagaaagag gtttttatat acagttaagt
      781 gagttaccaa ttataagcgt acaagatagt tatggccttg ttgatgtttt taaaaaccaa
      841 caagaaaagc ttttgaaaag ttctcaagag ttacttgatt atattggaaa ttatgaaaca
      901 ggggcaagta cagaaaaggt tcttgagttg ttagagaggg atataaagtg aaaaaaacag
      961 taaaagatat attttataat gcgatatatc aagtcttttt gattgttttg cccttactta
     1021 ctatacctat acttagtcgt agaataggtt caactggttt agggatttat gggtatgttt
     1081 tctcaatatc acaattttta atgactgtca ttgctgtggg aatgaatcct tttcgtattc
     1141 gaaatatcgc taaatcaagg aaagataaaa aagcactatc tttacaattc tggaatattt
     1201 actttcttca atttttaata ggcttgagtg tttcctttct ctatatagcc tatatttatt
     1261 tttttgatgt aaagtataaa aatttatact taattcaatt gatatttatt gcagggctaa
     1321 cattagatat ttcttggttt tttcaaggaa ttgaagaatt ttcgaaagtg gttattagaa
     1381 atacagttat caaattatca tcggtattgt taataatcct ttttgtttca actaaagatg
     1441 atttgtggat ttatgtgttc attacttcaa ttactaactt tttaggaagt ttagttttct
     1501 ggttgagtat ccacggaaaa gtaggtaaac cactctttaa ctctaatgtt tttaaacagt
     1561 tgtggaaacc agcttttatt attttgactc ctcagttgtt tatgcaagtg tacacaactt
     1621 tagacaaaac gatagttggg tattttgtgg atccaacagc attatcttat tatgatcagt
     1681 ctcaaaaaat tgctcgtatt atattggcag tcttaacatc aattactatt gttatgttgc
     1741 ctaaaatatc taaagcacag gcagagggaa aatcagaaaa tattttagtt tacacaaaaa
     1801 aatcttttga ttacacgatt attattgcat taattctttt tagtgtggtg tttatcaata
     1861 caaaagaatt tgttccatgg ttttttgggg agaatttcaa accgatgaca ataaacatgc
     1921 ttatttcttg tctaattata gttgttagtc caattggtgg agtttttgct aaccaatttg
     1981 caattgctat agaaaaagat aaggagtatg cttatccaat gatcattggt gcgattgtaa
     2041 gtattattgg aaatgtagct tttgtgccta cttatggcgc tgtagcggct acaatcgtac
     2101 tcgtggtagt agaattatta gtctttttct ttagagtatt ttttgtacga aaagaaataa
     2161 aaatatccta tttctttggg aagaacgttg tcttattttt tatagtcaat cttactttga
     2221 ctacttcggc ctattacttt cttccacaat tattctcttc ttcattttta gatatggcaa
     2281 ttaaatcaat aatgattttc attatttatc ttccgtttgt ttattttctt tttccaga
//
LOCUS       DAGCWU010000226         2309 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.233, whole genome shotgun sequence.
ACCESSION   DAGCWU010000226 DAGCWU010000000
VERSION     DAGCWU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.233"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            66..584
                     /locus_tag="MBZ71_002662"
     CDS             66..584
                     /locus_tag="MBZ71_002662"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBT4430556.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            712..1395
                     /locus_tag="MBZ71_002663"
     CDS             712..1395
                     /locus_tag="MBZ71_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBT4430557.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1704..1777
                     /locus_tag="MBZ71_002664"
     tRNA            1704..1777
                     /locus_tag="MBZ71_002664"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1738..1740,aa:Arg,seq:tct)
     gene            1936..2295
                     /locus_tag="MBZ71_002665"
     CDS             1936..2295
                     /locus_tag="MBZ71_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBT4430558.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          779 a          351 c          463 g          716 t
ORIGIN      
        1 tttgaaaaaa atttatcgtt attatatgat taagaaggat tgaataaaga aagaagttga
       61 aaacgatacc tgattacaaa aaaataactt ttataagttc tataacgttg attatatttt
      121 taattcttat tcttctcgtc gtcttggttc cccgaatttt tggtttgact ccttatatta
      181 ttgaggacaa agaaatgagt ccatattatg cacaaggaaa tctgatatat gtacgaccga
      241 aaaatacaaa tgagatttta gtcggagata ttattacata ttatgaaaat tcaggaaaaa
      301 aaatctcgac gcgtcgcgta atagccgttg aaaacaacca cgaagatttt tatacaaaag
      361 ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt atcggtagtc
      421 ccatctttca gattccatat attggactag tcctttcaaa aacggcattt gcatggattc
      481 aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg aatacctttg
      541 tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt taaaaacata
      601 aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa cactcatgca
      661 gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag tatgcgcaat
      721 gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg tacccagcaa
      781 aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc agaagtacca
      841 gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct tcagcctgaa
      901 cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc cacgacaatc
      961 ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga gaaagctaaa
     1021 aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg tcaagcagca
     1081 gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag tgcaaaatca
     1141 aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg cgttttgatg
     1201 gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat tccccgtggc
     1261 aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga tcatcccgat
     1321 ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa ggaatcagaa
     1381 acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga atcgagggct
     1441 caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga tacaggaaat
     1501 ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg ataaccttag
     1561 aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg ccaaaataat
     1621 tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa ttatctataa
     1681 ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca tccgccttct
     1741 aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt agaaactgag
     1801 gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa aggcaaataa
     1861 aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa actcatcaga
     1921 aaagaggaag aagatatgac gatactatct agaattttag tgactctagt tgcaattgaa
     1981 tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac aagtagggtt
     2041 tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt taagaaccaa
     2101 ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct ttcttccgca
     2161 tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt atatggcagt
     2221 ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt ggcattgctt
     2281 tctattttct tataaatata gaaaaaata
//
LOCUS       DAGCWU010000227         2304 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.234, whole genome shotgun sequence.
ACCESSION   DAGCWU010000227 DAGCWU010000000
VERSION     DAGCWU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.234"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="MBZ71_002666"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="MBZ71_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBT4430559.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          762 a          330 c          370 g          842 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagagac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
     2281 cctatacttt tacttggtat attt
//
LOCUS       DAGCWU010000228         2291 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.235, whole genome shotgun sequence.
ACCESSION   DAGCWU010000228 DAGCWU010000000
VERSION     DAGCWU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.235"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            110..1972
                     /locus_tag="MBZ71_002667"
     CDS             110..1972
                     /locus_tag="MBZ71_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4430560.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1972..>2291
                     /locus_tag="MBZ71_002668"
     CDS             1972..>2291
                     /locus_tag="MBZ71_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4430561.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETA"
BASE COUNT          818 a          342 c          453 g          678 t
ORIGIN      
        1 tctgtaatat tagttaaaaa gctataagtt gcacttagtt aagtgcaaac atttcatatt
       61 gtcgttagtt tagaaaacgc ttatattagc aatgtaagga gagatgataa tgcagcagct
      121 aacaaaaaga gaaaggcagc ttttgattta cttggtggaa atgtctgaac cggtaacaat
      181 caaaaagttg gctgaaaaag aaaatgttag tgttcggacg ataaaatacg atctagatga
      241 tataagagag tggctgaatg agcggaagca agtcctatat agcaaacgta gtcagggaat
      301 ttggatgaat attagtagct ctgatcgaat caaattaaaa agcgaattga tggatgttaa
      361 tcgttttgaa atatttgcag atcaaagtct ccgaatcaaa cgtttactaa ttatgctttt
      421 agtaacaaaa gaagctatta ctggaatgaa aatggccaac gcattagatg tcagcaaaga
      481 tacgatcatg aatgatttag atgtgctgga aaataatttt cttaaggaag gattgacctt
      541 gaatcgtcag gcgcgaaagg gtttttggat cataggagaa gaacgtctag ttcgattaac
      601 gattgaagag attcttcaaa aagagttcac ggattatgac atttataaat taatgtcatt
      661 gttattgaat ggcggtgaga ctgaatactt tgaaatgtat tctgctactg ctacacctat
      721 ccaagaggta tttaatcaag taatcattcg aatgcgacac ttactggaat ttgaaaatct
      781 agagaaactg aattacgcgg aattactaaa tattctgatt cgagtgacaa ttgctaccgt
      841 tcgcttacgc aaagaggcta cgattggtcg ttatcaattg gttgctgaac aagaattaga
      901 caaaaacgat ctttcttatc gcgtcatgca gcaggtcttt gattattatc agttgccact
      961 gttcgaggat gaatatcatt atatctatag tgatacattc gacacgattc accagcaaga
     1021 cgtcatgaaa ctagttgaga atgtgattta taaagtaagt aaagcaatca accattcttt
     1081 ttatcaagat ccacaattgt tgacgaatct gtatgctcat ttatccatgc gattatcgag
     1141 aaagaaaaaa tttgtcaatg aatataatcc ttttaaagaa gatatcaagc agaaataccc
     1201 ggaattattt gatgcgatca agcaagcagt tcgacaagaa ataagtgaac aaaatattct
     1261 gatcaatgat tctttcgtgg catatattgc cttacacttt ttggtttcct acgaaagaga
     1321 agaagatcta cgaagcgtta gagccgtcta catttgttca acagggttgg gtgtgactag
     1381 tctgatcaag caaaagatca atgaagaatt aaataatatc actatcgtgg catttgcttc
     1441 agtattgaat gcgcaagaaa tgattgctac caaagatcct gatttggtga ttagtatttt
     1501 tccaattaat ggcatcgatc gtcctttcat caaagtccat ccattaccca ctgaacagga
     1561 cttgaaaaaa attcaacaaa taataaaaaa aatacttgct tcttccaatc agcaggaaag
     1621 gcataacttg aatcttagag aaacgtccat caataaagat actttagaag actttagcaa
     1681 ggaattgatt ttgaaagctt atacgattta tgaacgttta cttgtcattt tggaagcaga
     1741 attgatccaa gaatatcgag aagctttttt gcttcatgta ttcttaatgg ttcatcgtat
     1801 cacatttgat caacaatata tggcagaagg tcgtcaagta gaccagatta ttttagacaa
     1861 aggaacactg gtaggaaaaa tcgaagcagt ttttgcagaa aataatttag gaatcaatca
     1921 gtcagaaatc agtgcattat tctcatatat aaaagaagga gcgtcaagtt agtgaaaatg
     1981 tcagacgagg ctttaaaaat tatcgaggaa agtcaaaatc gggaaaagtt aaaacaagtg
     2041 attgatctaa ttgaagagcg attagtagaa catgctattg taccaaccga gttgcagtgg
     2101 accattctaa ttaatcactt gaatgagatg ctgaaacgtt ctcaaagaaa ggaaacgatt
     2161 cccgtagtcg atcgggattt gttcaaagaa gtatccaaag aagccataac tatttctgat
     2221 gaaatagtta gaagtatcgg taatttaacc gatgatgaaa tatatgtttt gtcgattcat
     2281 tttgaaacag c
//
LOCUS       DAGCWU010000229         2261 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.236, whole genome shotgun sequence.
ACCESSION   DAGCWU010000229 DAGCWU010000000
VERSION     DAGCWU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.236"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            97..1710
                     /locus_tag="MBZ71_002669"
     CDS             97..1710
                     /locus_tag="MBZ71_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4430562.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          708 a          402 c          445 g          706 t
ORIGIN      
        1 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
       61 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
      121 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
      181 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
      241 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
      301 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
      361 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
      421 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
      481 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
      541 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
      601 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
      661 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
      721 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
      781 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
      841 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
      901 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
      961 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
     1021 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
     1081 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
     1141 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
     1201 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
     1261 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
     1321 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
     1381 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
     1441 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
     1501 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
     1561 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
     1621 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
     1681 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
     1741 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
     1801 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
     1861 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
     1921 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
     1981 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
     2041 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
     2101 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
     2161 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
     2221 gtgtattaaa taatttaatt tttttgttcc tatttattta a
//
LOCUS       DAGCWU010000230         2245 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.237, whole genome shotgun sequence.
ACCESSION   DAGCWU010000230 DAGCWU010000000
VERSION     DAGCWU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.237"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..535
                     /locus_tag="MBZ71_002670"
     CDS             <1..535
                     /locus_tag="MBZ71_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBT4430563.1"
                     /translation="TFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVH
                     FLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLD
                     DGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMD
                     LHDEWIYSSRKYINFDK"
     gene            592..984
                     /locus_tag="MBZ71_002671"
     CDS             592..984
                     /locus_tag="MBZ71_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4430564.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            985..>2245
                     /gene="aac(6')-Ie"
                     /locus_tag="MBZ71_002672"
     CDS             985..>2245
                     /gene="aac(6')-Ie"
                     /locus_tag="MBZ71_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HBT4430565.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTN"
BASE COUNT          890 a          239 c          384 g          732 t
ORIGIN      
        1 aacatttttt gaatacctaa aagaacgcgg tttacaaggt acggaactcg ttatttctga
       61 tgcgcacaaa ggattagtct ctgccattag aaaatccttc accaacgtaa gttggcaaag
      121 atgccaagtt cacttcctaa gaaatatctt taccaccatt cctaaaaaaa attcaaaatc
      181 tttcagagaa gctgttaaag gaatttttaa gttcacagat attaacttag cgcgtgaggc
      241 taaaaatcga ttgattcatg attatatcga tcaaccaaaa tattcaaaag cttgcgcatc
      301 attggatgat ggattcgaag acgcctttca atataccgta caaggaaatt cccacaatcg
      361 actaaagagt accaatctaa ttgaacgact gaatcaagaa gtacgcagaa gagaaaagat
      421 tattcgcatc ttccccaatc aaacatcagc caatcgctta attggagccg ttcttatgga
      481 cctacatgat gaatggattt attcttcaag aaaatacatc aattttgata agtagaaatg
      541 gtaaaaacat tgtatagcat tttacacagg agtctggact tgactgagtt tatggaagaa
      601 gttttaattg atgataatat ggtttttgat attgataatt taaaaggatt tcttaatgat
      661 accagttcat ttgggtttat agctaaagaa aataataaaa ttataggatt tgcatattgc
      721 tatacacttt taagacctga tggaaaaaca atgttttatt tacactcaat aggaatgtta
      781 cctaactatc aagacaaagg ttatggttca aaattattat cttttattaa ggaatattct
      841 aaagagattg gttgttctga aatgttttta ataactgata aaggtaatcc tagagcttgc
      901 catgtatatg aaaaattagg tggtaaaaat gattataaag atgaaatagt atatgtatat
      961 gattatgaaa aaggtgataa ataaatgaat atagttgaaa atgaaatatg tataagaact
     1021 ttaatagatg atgattttcc tttgatgtta aaatggttaa ctgatgaaag agtattagaa
     1081 ttttatggtg gtagagataa aaaatataca ttagaatcat taaaaaaaca ttatacagag
     1141 ccttgggaag atgaagtttt tagagtaatt attgaatata acaatgttcc tattggatat
     1201 ggacaaatat ataaaatgta tgatgagtta tatactgatt atcattatcc aaaaactgat
     1261 gagatagtct atggtatgga tcaatttata ggagagccaa attattggag taaaggaatt
     1321 ggtacaagat atattaaatt gatttttgaa tttttgaaaa aagaaagaaa tgctaatgca
     1381 gttattttag accctcataa aaataatcca agagcaataa gggcatacca aaaatctggt
     1441 tttagaatta ttgaagattt gccagaacat gaattacacg agggcaaaaa agaagattgt
     1501 tatttaatgg aatatagata tgatgataat gccacaaatg ttaaggcaat gaaatattta
     1561 attgagcatt actttgataa tttcaaagta gatagtattg aaataatcgg tagtggttat
     1621 gatagtgtgg catatttagt taataatgaa tacattttta aaacaaaatt tagtactaat
     1681 aagaaaaaag gttatgcaaa agaaaaagca atatataatt ttttaaatac aaatttagaa
     1741 actaatgtaa aaattcctaa tattgaatat tcgtatatta gtgatgaatt atctatacta
     1801 ggttataaag aaattaaagg aactttttta acaccagaaa tttattctac tatgtcagaa
     1861 gaagaacaaa atttgttaaa acgagatatt gccagttttt taagacaaat gcacggttta
     1921 gattatacag atattagtga atgtactatt gataataaac aaaatgtatt agaagagtat
     1981 atattgttgc gtgaaactat ttataatgat ttaactgata tagaaaaaga ttatatagaa
     2041 agttttatgg aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg tttatgccat
     2101 aatgatttta gttgtaatca tctattgtta gatggcaata atagattaac tggaataatt
     2161 gattttggag attctggaat tatagatgaa tattgtgatt ttatatactt acttgaagat
     2221 agtgaagaag aaataggaac aaatt
//
LOCUS       DAGCWU010000231         2179 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.238, whole genome shotgun sequence.
ACCESSION   DAGCWU010000231 DAGCWU010000000
VERSION     DAGCWU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.238"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            349..804
                     /locus_tag="MBZ71_002673"
     CDS             349..804
                     /locus_tag="MBZ71_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430566.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(966..2138)
                     /locus_tag="MBZ71_002674"
     CDS             complement(966..2138)
                     /locus_tag="MBZ71_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4430567.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT          696 a          433 c          393 g          657 t
ORIGIN      
        1 tgtatcaaaa cgggattaga ttaagtttcc tcaatcacta ttcataacac aattattata
       61 acaagtaagt aaacaattga aaaggacatt tattgaaaac ggttgttttt tacttaagga
      121 ggaaatttag tgaaaaaatt agttgcattg tttttggtcg caggaagttt gttgagtggg
      181 tgtggtgcta ctacgtctac tactcaaagc acaagttcag ctcaggcttc cagcagcagt
      241 accacgatct ccgaaagtac ggcagattca tcgaaagaac aagaacaaat tgaaaagaaa
      301 aaagaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaaagt gacagaagcg
      361 gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag aaaactatgc aactgcttca
      421 gcagctatcc aagcgctacc tgaaaacaaa caagagttga caagccgtct tgctgcaatt
      481 gattccacaa tcaaagcaaa agaagcagaa gaagaacgga aaaagcaaga agaaattgca
      541 cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac agcaacaagc agaacaggcg
      601 cgacagcaag cagcagctga acaggcctct cagcaggcac aagccgcagc tcctgagcaa
      661 aataatgaac aaactgtcta tgtcacaccg accggttcaa aataccatac acataaatgt
      721 ggaaatggta cgtatacccc tgccacttta agcgaagctc aagctcgtgg tttaacccct
      781 tgctccaaat gttatggaaa ctaattcaaa agttatacta taataataaa attcttctga
      841 aacataaaaa aatagaagct ggcgttccag atagtaaacc aaaaatagca gtttacacgt
      901 ccatttcgtc agcttctctc ttttttcatt tagtctgttc cctctgcttt tttggacatc
      961 ttcattcatt ctttctcaat caaatagtac acctcttttt cttttcgcag tttatatctt
     1021 ccttttttca atttcattga aaactgtttc gaatgtttat gttttacatg gattttaagc
     1081 aggagctcct catgttgcaa gcgatagttc ctgcctttta aatcagaaaa tctcactagt
     1141 ccgtattcat cagaagcttt cacaaatgga actttttctc tagtaatata gtgtttgccg
     1201 ttgagcgttt ttacagaaaa gaccattcct tcgattggtc gtccgtttgt gttctttaca
     1261 taaaaagata aaggatagtt tcgcttagcg atggccaaag aatctaaata agcgatgagt
     1321 agccaaacta agccgattga tccaagtagt ataccgataa accaaataaa aaatttccat
     1381 tttttccatt gatcaattcc ttggatcgct ttttttgcct cttttgcttg ataaggaata
     1441 cgatgccccg tcacaagcag tcgatgtgta ttgaccatat aaggagtaca ggtaagcaaa
     1501 gtcacgagat ctctgccttt ttggataccc aaagaatcta cttcatcagg taagaccact
     1561 tggatttttt ctacttgata cgccaatgtc tttccattga tctggatgaa aaactggtct
     1621 ccttttttca acttaggcaa atctgtaaat aattttgctt ctgggagtcc acgatgccca
     1681 gaaagaacgg catgggtgtt tttcccgcca gtcggataag aactttcttc caataatgac
     1741 gttccttttt gcaaaaatat ttccgttgtc tgatcgaata taggtaagct gacattgatt
     1801 tttgggattt cgatgactcc aagcatatgt tccataaaaa aggcattact aggtaaatct
     1861 tttaatgctt ttgcatcaac tgcttggttg aaactgccta tccctggtga tacgttttgc
     1921 tctgccaact gttggttttt ctttgtcatt ttttcctgct gtttttttat ttcctctgaa
     1981 ttcttattcg atgcttgctt ttgataatgg atgatcattt gctgggcaag aaaatcattc
     2041 agtgaatctt ctacaaaagg ataagaaaag atcccgatcc caacgactaa aatgattgcc
     2101 atgaagatgt ccagtattct tattctgctt ttattcatca tgtccaccta ttttctaata
     2161 tgtatatgaa gaggttgtg
//
LOCUS       DAGCWU010000232         2112 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.239, whole genome shotgun sequence.
ACCESSION   DAGCWU010000232 DAGCWU010000000
VERSION     DAGCWU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.239"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(642..932)
                     /locus_tag="MBZ71_002675"
     CDS             complement(642..932)
                     /locus_tag="MBZ71_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430568.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1140..1832)
                     /locus_tag="MBZ71_002676"
     CDS             complement(1140..1832)
                     /locus_tag="MBZ71_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBT4430569.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          600 a          392 c          310 g          810 t
ORIGIN      
        1 gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa atctacgata
       61 gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg acctatggat
      121 tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata ttgcttttaa
      181 aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag gtttaactgt
      241 cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt acgactatga
      301 taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat cctacaattc
      361 cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc cccattgagc
      421 gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc ctcaaatgag
      481 tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata tatagattga
      541 ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc catctttcca
      601 aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat tctttcatct
      661 tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat tttgggtttc
      721 cctaacacat gataggatct cacaacaaat tgctttctat tattataata acctgtaacg
      781 actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga agcaatcaaa
      841 caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg caaagtacgg
      901 atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc attatacgaa
      961 catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt tgtctattac
     1021 ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact aaaaaaaaga
     1081 ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt taagaaagat
     1141 tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc ttccacttca
     1201 tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc agcagttgct
     1261 tcgtctaatt tcttttgtgt atctgttaac tgagtttcta catttgtttt ttcttgtgtc
     1321 agtgttgcga tttgttgatt caaaccattt acagtatttt gtaattctga aagttttgct
     1381 tgtgcttctt gtagtcttgc tgtccaatct gcattcagat gattgatttc atcatttttt
     1441 tggttgatct catctagttt gacattgatt tcattttgtt tgctttgtag ttgtgcatcc
     1501 ttgctgacta attgactttc taagttttgt ttttctgctg ccgattgatt tttttggttt
     1561 tctaattcat ttacttttgt ttggtattct tggacatctg cctttgaatc ttgcaatgtc
     1621 tgttcttttt gtgctagtcg agcggtaaga tctgtaatcg cttgctcttt atctgtcaat
     1681 gtatgatcaa gctgtgtcag attctcattg attgtatcaa tatctgtctc tccaccccac
     1741 aaaataattt catttgcgta ccgcgtgcct gtgtagccaa ttagacatag aaatgcaacg
     1801 acaatcccac cagtaataaa tttctttttc atttgcatag ctccttaatc caatactttt
     1861 acatgctaaa agtaagtgat ctcaaacaca atgtcaagcg tttttaaatt acatatgtaa
     1921 ttttaatata aatactatca aatactcatt taatcctcct tttttatcta tttaaaaaat
     1981 cttttttata gagaaaaatg caggttaata ctttcttaaa taaatattta tggtgaacaa
     2041 aaacaaaaaa gcaagtttta ttaaacgtaa cccttttcat ttcatagatt actgtattta
     2101 ttacaaaaaa cg
//
LOCUS       DAGCWU010000233         2104 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.240, whole genome shotgun sequence.
ACCESSION   DAGCWU010000233 DAGCWU010000000
VERSION     DAGCWU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.240"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            169..1539
                     /locus_tag="MBZ71_002677"
     CDS             169..1539
                     /locus_tag="MBZ71_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HBT4430570.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="MBZ71_002678"
     CDS             1560..1844
                     /locus_tag="MBZ71_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430571.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DAGCWU010000234         2067 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.241, whole genome shotgun sequence.
ACCESSION   DAGCWU010000234 DAGCWU010000000
VERSION     DAGCWU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.241"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            680..1012
                     /locus_tag="MBZ71_002679"
     CDS             680..1012
                     /locus_tag="MBZ71_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBT4430572.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1631..1972
                     /locus_tag="MBZ71_002680"
     CDS             1631..1972
                     /locus_tag="MBZ71_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBT4430573.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          721 a          277 c          434 g          635 t
ORIGIN      
        1 aacgtagcct tttacgtcat aagtgttaaa agaaataatt aatttcaaat atagaaaagg
       61 gaacgtatgt ttgcatactc cttttttttg agttataata atacgcgaag agagataatt
      121 tcattattta aacaagctaa aaatcttgat attacttctt ttcaggcaat acgaatatta
      181 ttcgcttaca ttttttgtat aaatttaatt gtctgctatg cttgtgatga attgattttc
      241 gttctgcact tttggatgat aaatttggac aattgaaaat acaaaaaaaa gaaaggcggt
      301 atgaactcgg aaggctggta accttcgtca gcgttcatac acggcattaa gatttggccc
      361 ttaatgcgtt aacctttcag tgttacggta ttacccgcaa tggatttagt ataacagata
      421 ttcaaaagtg ataccacttt ttttggctgt gttaaggtta aaaatacgtg atattacgat
      481 gaatctatgc ctaagcacaa tgttataaaa atgtccgtgt ggctctttgc cgtatgacga
      541 acgctgaaat tagagggaac gcatgtttgg atgcgttccc tcttttttgt ttgtcatgaa
      601 cgaaaatcaa ttctctattt ttgaaaaata acaagaaaaa cttgttaaaa atatttaaag
      661 aaattgggtg gtttatttta tgtcatatac aggaattttg agtctcaaag atatttgtca
      721 ctacggtaaa cggtgtaccg ctacagagaa aatcacgaag aaactatcaa ctggccaaag
      781 taaaaccgtt gtgcagtgta agaaatatat aatccagaaa gacaaagtct cagaggaaat
      841 gatttattac attgggaaac aaaagcaaat aattttaaaa gatcctatcc ccttaaaaga
      901 gctatatcca acaatcaaac atgtttatga ccaaaacggt gtactgattg gtcgaagaaa
      961 aaatggagtc ttgcgttgta ccgcaaaagg gatggggcgt ttgatcggct gaattgatca
     1021 ttctcataac ttgtatttta tcgtttttcg cttgcttgaa aatattgtcg agacttctaa
     1081 aggaacttag agtcactaga aaaataagga gtagacttga tctatcaaca attcccttcg
     1141 gggagtgacg gggtaggttt taacgattgg taacgtaaaa aattggtcaa tcgagtaaat
     1201 gcgcttgttt ggatagtggt gaaatgaatt tgtgtaaata ataagcgcag agtaaacaag
     1261 agggaattta gaagttagac aagttagaat agcgtctaga ttaatcatga gcagctacta
     1321 agggatctag actttgacgc aaactcatgg aataaggagt aggtcttctc gtaatgggga
     1381 ggacgcacaa gaaattgtgt cgtgtgctgc agtacatgta gatacttgcg aggacgttga
     1441 ttattggatc aatcgagcat ttaccgctgt ctactagggt ttctcgtttc ggacgccata
     1501 aacgagtcga aattttttat gtatttttaa gtaaacgaaa aacgataaaa tatgaacaag
     1561 tcctgaataa agtgaaaaaa ttgatataaa acatagagaa aatgaaattt acgtaatgga
     1621 ggaatgacaa atgaaaaatt atgatccgaa tatacgattg ggcacacata cgattaaggt
     1681 ttctttccaa cgttgggatt acaaaggatt tgtgacgttt agaagaggcg gaaattgtaa
     1741 aggattagat gttttagcat tagacgagga tgatttatac gatcaaacat tgactgataa
     1801 tccaattgga ttcggcttgc tacctgaaga tgatgaaggt aatgaatggt ttaaaatgac
     1861 gcttatgaat gataatggag acgaattatc tgttgaagat acttggtcat atttgagtga
     1921 ttatattgtc agctttgaaa tcattgaatt tgtagcggat aaagaagaat aaaaactgga
     1981 ggaataaatt ttacagataa aggcttagaa ggagtcacga tgattaataa aaaggtagta
     2041 ttaccttagg cattgagtaa ggaattt
//
LOCUS       DAGCWU010000235         2055 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.242, whole genome shotgun sequence.
ACCESSION   DAGCWU010000235 DAGCWU010000000
VERSION     DAGCWU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.242"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            25..468
                     /locus_tag="MBZ71_002681"
     CDS             25..468
                     /locus_tag="MBZ71_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430574.1"
                     /translation="MVVEDYDKVDGRNALNTDAKGLSLGLAQWNDRGKVDISGKVWRH
                     TGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENPVVDIIGLQGNRM
                     TVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            501..>2055
                     /locus_tag="MBZ71_002682"
     CDS             501..>2055
                     /locus_tag="MBZ71_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4430575.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKVSNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDPDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEFPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPSFQGKTPEVRELHMQVMPIVESLS
                     RQILELLDNE"
BASE COUNT          758 a          372 c          440 g          485 t
ORIGIN      
        1 acaaatttaa aacataaacc agtaatcgtt gtagaagact atgataaagt tgatggaaga
       61 aatgcattaa atacggacgc aaaagggctt tctctgggcc ttgctcagtg gaatgatcgt
      121 ggaaaagtcg atatctcagg caaggtatgg cgacatactg gagaaaaatg gtcacgacag
      181 tcggaagaat taccaatcac acgtatttta gatttagcga tcttagctgc gcaaggaagt
      241 ttatacttcc aagatgctta tcgctatgaa aaattttatg atccagaaaa tccagtcgta
      301 gacatcattg gattgcaagg gaaccgaatg acggtagaag tagataccaa aaatccaaag
      361 atcgatgaag acatcatttt gtattacgat acactacaaa aagacggaga actgattggg
      421 gaacgattcc gtattttaaa acgtctttta gatgagctag gatattaata gacagagatg
      481 atcaaaaggg gaagagaagc atggagaatt tctcactaac agaaaaccga accttttcac
      541 ggctagaaaa gcaaatgatc tggaagaaac cgaaaagcca tcggacaagt gcagaagaat
      601 ggcggatcgc ctccgaaatt aaagggaatt ggcaagatcc tgaaatgaaa gtcagtaacg
      661 tacttttgga aggagatgcc gggtcgggga agacacaatt agccaaagca ctatctgctg
      721 atctgcaact tccctataca aaagtgacct gttttgcaga tatggacaaa tcagatgttt
      781 tcggagcctt attacctgta gtagattcta ataaggaaca agaccaagaa ttactagaag
      841 ctatttacca aacagataca cttgaagctg tactgacact gatccagaac cattacggca
      901 ttgatccttt ttctgctaaa gaaaaattag cagagctagt tcaaaggatc gaagctgatt
      961 atccagatgc aatccattac agctattatc cttctgaaat cgttcgagcg attgaaaaag
     1021 gatatttatt agagattcaa gagccgactg tcatccgaga tgcctctgtt cttgtggcac
     1081 ttaactcagc gcttgaacca aatgggatgt tgaatttacc gacaggaatc attcgtcgcc
     1141 atccagattg cgtcattgtg atcactacaa atcgaaatta ccaaggaaat cgtccattaa
     1201 atgaatcttt aagagatcgg atgcaacatg cggaaaaaat ggatcttcct gccttagaag
     1261 tcatggcaga acgtgcgatg gcgaaaacca tgatccaaaa acaagaactt ctcttaaaga
     1321 tggcagagat tatccgtttg ttggatgaaa ctgcaaaagc taacgccatc aaaggtgtag
     1381 caggaatgcg ttcttatttt tattgggcaa atacctataa gcaaggacaa gatttactcc
     1441 agtcgattta tccaaaagtt ttgtataaat tatccactga tccagatgaa ttggcaattc
     1501 ttgaacaagc tttgtctgac agtggattgc tactggaact tcaggacata ttgagaaaag
     1561 aaaaatgggg tcatcctcaa tctgagcata caaaaggaag aaccattagc gccgaagaag
     1621 caaatgaacg aaacatcact gaaaatgctg aagaaattct gcgcttagat gaaaagaaat
     1681 cgttgtctgc tgaagaacca aatgagggag aagagaaaca agaagaaaca atagaaaaaa
     1741 acgagtttcc tcctcaaatg caaaaagaac gcagtagcga tatcgaagga aaaaatcaaa
     1801 aagaacaaca aacaggagat gattctcaag aaatgtctgc ttctgacatg gaagcacatg
     1861 ataaggaaat caagaagcaa ctgaataaag aagcgcgaca aatcatgaag gaaacgattc
     1921 atgaaaaaga aggtctcatc gttcaccgac catctttcca aggaaaaacg ccggaagtac
     1981 gtgagctaca catgcaagta atgccaatag tagaatcact tagccgacaa attttagagt
     2041 tactggataa tgaac
//
LOCUS       DAGCWU010000236         2037 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.243, whole genome shotgun sequence.
ACCESSION   DAGCWU010000236 DAGCWU010000000
VERSION     DAGCWU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.243"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..914)
                     /locus_tag="MBZ71_002683"
     CDS             complement(<1..914)
                     /locus_tag="MBZ71_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBT4430576.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNHTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="MBZ71_002684"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="MBZ71_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBT4430577.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          678 a          369 c          395 g          595 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatggttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatatgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caattcttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa accagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttattta
//
LOCUS       DAGCWU010000237         2036 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.244, whole genome shotgun sequence.
ACCESSION   DAGCWU010000237 DAGCWU010000000
VERSION     DAGCWU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.244"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>2036
                     /gene="acm"
                     /locus_tag="MBZ71_002685"
     CDS             <1..>2036
                     /gene="acm"
                     /locus_tag="MBZ71_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBT4430578.1"
                     /translation="PVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQN
                     IQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDS
                     VEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDML
                     PEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEE
                     GIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSE
                     AWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELR
                     RADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSI
                     NENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETK
                     RLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKE
                     VISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRD
                     GQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKI
                     RNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAV"
BASE COUNT          801 a          337 c          401 g          497 t
ORIGIN      
        1 cctgttcatg tctatgcgga tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc
       61 gtggacccaa cgaatattac agatggagga aacataaaag taaaattctc gtttgacgaa
      121 aaaaaacaaa atatccaacc aggtgattat ctttggatca attggccaag tgaaggaaat
      181 attcgcggtg aaggattcca aaaagaaatc ccattaatga ttgaaaataa aaatgttgga
      241 actttaacgg tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat taaaaaccta
      301 gactcagtag aaggttgggg gcaatttgag atacaggcca gaaacgtaac cgataccggt
      361 gaagaaaata cgggaccatt caccgtgacg agcggtgata aaacagctac tgtaaatgtg
      421 acaaagccag cttctggttc ttcttctagc gttttttatt ataaaacagg agacatgcta
      481 cctgaagata ctaagcacat tcgatggttt ttgaatatta acaataacgg cacttatgtt
      541 gaacaaccag ttaaaatcag tgatgaaatc caaagtggac aaagattaga tccaagtacg
      601 tttgaaatta atcaaataca cttaggtgaa cagaaggttt atagaggaga agagggaatc
      661 caacaatttt tacaagattt ccccagcgct acatttaatt tttcagtaac tgataattac
      721 attgaaataa ctataccaaa aaactttgtc aatcttagga aaataatggt gagttataag
      781 acgatcatag aaaacccaga acaaataaat tttgagaatc attcggaagc ttggtttaaa
      841 gaatttaata aacctgctgt tgatggagaa agtttcaatc atacagttaa aaatatttca
      901 gcttctggtg gagtcaacgg tacagttaga ggcgaattga agattttcaa atatatcaat
      961 gacacagaga ttggtatccc aaatgtgact ttcgaattaa gacgagcaga tgaacagcct
     1021 attcaaggac agtctagtat cctgttgact tcgaatgaac agggagaagc tagtatcaaa
     1081 ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag cacccaattg gattgatttt
     1141 gatccactaa gttcaaatga actcaaattc tccataaacg aaaatgatac agagggagtc
     1201 agtttaccta tttataacaa gaaaaaggtt acgaacatca cagcaactaa aatttggaac
     1261 ggtggaacca ctccaagacc gagtatttat tttaaattat tcagagcaag tacaaacaat
     1321 tgggaaccag ttcctgatgc agaaacaaaa cgattagaca atggaataac ttcagtgact
     1381 tgggaagata tccaacagta tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa
     1441 gtagatcaaa acggaaatga ttatgtccct tcaggttatc aaaaaattga aaacgggcta
     1501 gtcgttacaa atgagaataa agaagtcatt tcagtctcag gacaaaaaac atgggaagac
     1561 aatgagaacc aagacggcaa acgaccgacg acgatcacag taaatctatt agccgacggt
     1621 caaccgatcc aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat
     1681 ctgccaaaat acagagacgg gcaagaaatc atctatactg taacggaaga caacgtgccc
     1741 gaatacagta cgacgattga cgggtataac atcaaaaaca gttatacacc ggaaaaaaca
     1801 aatatatcta taccaggtag ccatataaca ccgtggaaaa attttccaga gaagacagag
     1861 agagagaaaa ttagaaatct attctctcaa aaattgcaaa tatttgctca tacaggagaa
     1921 tccagcgaac aaagaagcaa ttatattagt aagagagctg tacctaaaca gataacaaat
     1981 aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat ctagttttgc tgtaat
//
LOCUS       DAGCWU010000238         1952 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.246, whole genome shotgun sequence.
ACCESSION   DAGCWU010000238 DAGCWU010000000
VERSION     DAGCWU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.246"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="MBZ71_002686"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="MBZ71_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBT4430579.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="MBZ71_002687"
     CDS             852..1040
                     /locus_tag="MBZ71_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBT4430580.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="MBZ71_002688"
     CDS             1053..1610
                     /locus_tag="MBZ71_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4430581.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="MBZ71_002689"
     CDS             1642..1884
                     /locus_tag="MBZ71_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4430582.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          715 a          304 c          404 g          529 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc gg
//
LOCUS       DAGCWU010000239         1928 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.249, whole genome shotgun sequence.
ACCESSION   DAGCWU010000239 DAGCWU010000000
VERSION     DAGCWU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.249"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..367
                     /locus_tag="MBZ71_002690"
     CDS             <1..367
                     /locus_tag="MBZ71_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBT4430583.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..770
                     /locus_tag="MBZ71_002691"
     CDS             567..770
                     /locus_tag="MBZ71_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430584.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1330..>1928
                     /locus_tag="MBZ71_002692"
     CDS             1330..>1928
                     /locus_tag="MBZ71_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBT4430585.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          625 a          293 c          454 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggttcacct
      661 tagggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcggtgaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa cgaaataaat gctggcagcg
     1021 ccattctatt tcggcaggag gaggctcaag gggttcgggg aatgaaattc cctgaggtct
     1081 ttttattttt tctagaggag tgctataatc aaagaaaatg agaaaaaaga gtaaaaaaat
     1141 agtcgagcag tgttgacgca ctctcgacct ttacggaaac tacttcacct agttccgtaa
     1201 ctcaagttca ttatacgaat atttttaggt ttcttcaagc tagagatttt tggatgtgga
     1261 tttttagagt tacgtgaact ctggaaattc tttccaagaa tctctttttt cgttgaggag
     1321 gagctatttt tgagtaaaaa aggcataatc gtaaatgagt gggaaaaact taaccgtatt
     1381 tctaacaggc ggttaagtgt tgacgataag gtacaccccc taaaattaag ttggtcaatc
     1441 gatcgaatta caatcgtcgg aaaactgaat gataccatag cttattggac gcaaaatgga
     1501 gttgtgtttg tcgattttga gcaattaatg cgtttgaatg aaaatcaagg gtatttgaag
     1561 tcggttggta caaatggttg gcaattattt gaccaatatg gagaagatat cgcctatatc
     1621 gaaatgttaa aatttcaccc tgacaaaggt cgtattgatt ttaatccaaa taaaattggc
     1681 gagtttttaa aggggtcgat gaaacaattc attcatgatt tgtttctgga tgcgcatttc
     1741 tcacgtgcag atattgcttg cgatattctc ggtgttcctg atgattttat tacccagtat
     1801 cgaatcgttg accctgtgtc ttttaagcca atttatggcc gttctggcaa attagaaaca
     1861 gcgtactggg gtagtcgtgc cagcgagcga caagtgcgga tgtacaacaa aaagcttgaa
     1921 caggaacg
//
LOCUS       DAGCWU010000240         1906 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.250, whole genome shotgun sequence.
ACCESSION   DAGCWU010000240 DAGCWU010000000
VERSION     DAGCWU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.250"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..73
                     /locus_tag="MBZ71_002693"
     CDS             <1..73
                     /locus_tag="MBZ71_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBT4430586.1"
                     /translation="LFFAVFLFLGKNSLKQTKKKELV"
     gene            complement(103..612)
                     /locus_tag="MBZ71_002694"
     CDS             complement(103..612)
                     /locus_tag="MBZ71_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBT4430587.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(724..1722)
                     /locus_tag="MBZ71_002695"
     CDS             complement(724..1722)
                     /locus_tag="MBZ71_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4430588.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
BASE COUNT          578 a          358 c          338 g          632 t
ORIGIN      
        1 attatttttt gctgtttttc ttttcttagg aaaaaatagc ttgaaacaaa caaaaaagaa
       61 agaactcgtc taaatagacg ttttctttct ttttttgttg aattattgag taaggcgact
      121 tcttttttct ggttctttgt gtgctaatcg actggcagca gcagcggcga tcgctccgac
      181 gatatcatct aagaaggtat ggacatgaac gccatcgtgg ctgtttagtt cttttaaaat
      241 accaggtttc accttgtcga tataaccata attcgtaaag ccgatcgtac cgtaaacatt
      301 cacgattgac aaggctagga tctcatcgat accgtaaagg ccttcgtcat cttcgataat
      361 atgttgcaac ggactcatca attttttctt ttctgctaat atatctaatt ggattccagt
      421 gataatggcg ttatgtactt cacgctttgt tagcacgctg ttgacacttt cagtacaatc
      481 cgccaaagtc aaatcagaaa tatagtcttt ttgtaaaaac atgacaagtt gtgcgatatc
      541 ttctacagtt acgccacgtt ccttcaatag ttctcttgcc ttttcttcta aagtctcagt
      601 tttgacgacc atattattgc tcccccttta ttttttcttt ctctatttca tgttaacgga
      661 acatgaaaaa aatgcaagaa actgctttcg ggtttaactt gaaatgcaaa gagatataaa
      721 aaatcaacta ggacgaagat ttttatcgta taagcttgtg ctgtgaccag gctgagttcg
      781 ttcctttgga ttaatatagt gtaaagcgtt gttcactgca gtaggtgctt caccgagccc
      841 agtagcaatc agtttcactt ttccttcata ggtacagatg tcaccagctg cataaacgcc
      901 tggaatagaa gtagacatat ctgattttac agtaatggca tttctggtac tatcaagtcc
      961 ccaagaagaa agatgttcta aattagaaga gaatccataa ttgacgatca gagaatcaat
     1021 catcaaatca atcgtctcgt cacttttcac tttttttaac cggatatctg tcagttcccc
     1081 attattgcct gataatccat cgatgagata gggagtaagc aagttgacag atgaagattt
     1141 caagcgtgat acactatgtt cgtgcgcacg aaattccggg cgacgatgaa tcaagtaaac
     1201 ttcgcttgca ataggctcta acatcaacgt ccaatcgata gcagaatcac cacctccagc
     1261 aatagcgact ttttttccag catacttcat caaatcattc acgaaataat ccaatccgtg
     1321 gttttcaaat gattcagcat tatctaaatt caattttctt ggctggaatg aaccatttcc
     1381 taaagctaaa atgactgctt tggaataatg gatgccttta tttgtagtaa tctcgataac
     1441 ttcgtcttca cgagtaagcg agagaacttc ttcttttaaa tggaatgtat ggttaaaagt
     1501 agttaattgt ttttctaatt gatcgattaa ttcacttgct tttattgctg gatacccagg
     1561 aatatcgtaa atatattttt cgggatacaa agttgctaat tgaccaccaa gttgaggtag
     1621 actgtcaatg atttttgttt tagcttggcg cattcctgcg taaaaagcgg caaacatccc
     1681 tacaggacct gcaccgatga ttgtaatgtc atagatttcc ataaaaattc ctcccttttc
     1741 acactatagc agaatttaat tgagaatgct tctcaaaaat gtttgaattc gaaatcgaaa
     1801 cggtaagtta tagaatgtat ctagtgatga aaaataaaag aaaacaaatc tagtattttt
     1861 acgagaggtt aggtattatg ttattattat tatgaagtat gcaaat
//
LOCUS       DAGCWU010000241         1884 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.251, whole genome shotgun sequence.
ACCESSION   DAGCWU010000241 DAGCWU010000000
VERSION     DAGCWU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.251"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(379..876)
                     /locus_tag="MBZ71_002696"
     CDS             complement(379..876)
                     /locus_tag="MBZ71_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4430589.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(916..1725)
                     /locus_tag="MBZ71_002697"
     CDS             complement(916..1725)
                     /locus_tag="MBZ71_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4430590.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          545 a          347 c          327 g          665 t
ORIGIN      
        1 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta
       61 tgtttttact tggctgttct tattactgca gatgcaagct tttgaatacc gtttaaggaa
      121 gaggttgtga aatcagcgtt ttgacctgag tattagctta acaaacggaa aaatagttcc
      181 tcatattttg ccgtttgtta agctaatacc gagggtcagc tgattgaaca ccatttaaga
      241 aagaggtcgt gagcctgccg tttcactctg tgtcataaaa aagccaatct actttgatag
      301 aaaaagcacg taggactaaa acagttctgc gtgctttttt tattgtgtgt taaatatcgt
      361 aaatgaagat agatattttt atgattcgac gatagagacg ttgaaatctt tcaacagctc
      421 taaagcggat tctccgtgtt ttctactgga gactaacatt ggatcaacaa aaaagtctgc
      481 ttcaggaaaa gcactctgca ggcctatcag attagagagt acacacaagt ttgtttcaat
      541 cccgactact tcgatctctt tttgttcttc tttgctttca aataaagttt cctggatttc
      601 agctaaagtt tcaggtggaa tcgtaaagta atacttttta atcactcgtt cattttcttg
      661 tggagaaaga agagggatca attgcaaatc ttctcgttct tcttctttgt ccttcagttc
      721 aactggtata tcccgtgtga agagaatata ttcattattt tggcgtgctt tagctaatct
      781 ttgagcaata cgttctgcca attgatctga ctctggaata taaaattcgc tgtttggatc
      841 aagaatatga ttttgcatat caataataac taacattttc tttcctccta cattcatatc
      901 gtcattttat tctttttaat agaagttttt taaaatataa gcaacaccag cttcttcatt
      961 cgtcagtgtt acttggtttg caatttcttt tacttgtcgc tgtgcattcc ccatcgcaat
     1021 actcaacccg ctgtaattca acatagcaat gtcgttttct tgatcgccaa aagccatgat
     1081 gtcactcgat tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag ctttagtgat
     1141 acctgtaggc gtgatttcta agaaatgcgg atcagagaat actgattgga taccatctgc
     1201 ttgcagatct ctttgtagct tctgtagttt ttcttcactc ttatctgtta atgctaattt
     1261 aagatagcta agagaaggat cttccaacat atttaaaatt tgatcaaaag aatggttctg
     1321 taaattgaaa tgttcataat aagggtcatc tttgactaga tttgattcat cgaatgtcag
     1381 ctgatcatct tgggtttctc ggctagcaat gatggatatt ttcgcttctt gagcatagaa
     1441 gaaggctttt ctcagtgtat cgctatctat ttttgaacgt ctgagaactt tcgccatttt
     1501 tccatccttc atttcagcaa tcagtgaacc attggaagaa atcaaatagc caccgattcc
     1561 taattcggaa aaaatatctt tagcagaaat gctgttcctt ccggtagcga cggttacgat
     1621 tccgccattt tcatcaaatt tccgaagtgc ttggatattt tcttctggta tctttccatt
     1681 tcgatcaagc aaggtaccgt ctaaatcaat cgctgctagt ttcattttat cttcctactt
     1741 tctttttaca tatctatttt cctgttacct taagattaaa tgagaattta aaaaaatgaa
     1801 actaacttgc tttgtcatta tgaatttttt caaaaaacat tcttaagaaa aaggtggcta
     1861 tatatagtag tttttggtca atac
//
LOCUS       DAGCWU010000242         1877 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.252, whole genome shotgun sequence.
ACCESSION   DAGCWU010000242 DAGCWU010000000
VERSION     DAGCWU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.252"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(367..1659)
                     /locus_tag="MBZ71_002698"
     CDS             complement(367..1659)
                     /locus_tag="MBZ71_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4430591.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          586 a          375 c          311 g          605 t
ORIGIN      
        1 tgtatgggct actctgtgat acttattagc cattaaacta ttttacccac atagcatata
       61 ttttgaaatc gttcattccg taaattcaac taaatatgcc taaaaaaata aaaaatctaa
      121 gatagaataa gatttattca gtcttagatg gatttatagc aactttttcg taacaacttt
      181 aaaaaatacg aaaagaaaga gctgttttac tctgactacg ttgctaaatt gtcgtttaat
      241 cattcattta aaccaaatct ttttttatag aattcgtttt aatcctcctg aatccaaaaa
      301 ctatgaaaac cgtaattaaa tagttaagag tttttaacca ccataattct ctcggctatt
      361 ttgaaatcag ataaattttg atcggacaca tagttgaata aacctaccgt tgttacggaa
      421 ggtaaatcgc atacttttca ttctagagga gaaggtctcg ttgatcatta actgattaag
      481 caccatagcc cagttttgaa ttcgaccacc tgaccacttc gcgtgtaatt ctttcgtgcg
      541 taaataaagt agttttagga gtgcattctc attagagaac gctccttttt ttgtgacttt
      601 tctgaagctg gagtggacac tttcaaccgc attggtagtg tacataattt ttcgaatggc
      661 actaccataa tcaaatagtt gttcaacatg tgcaaagttc cgtttccaga catctacagc
      721 accagaataa tgagaccacc gattttgaaa actgtcaaaa gcagcatgtg cagcgtttag
      781 agaagaagca ccgtagaact ttttcatatc tcggcagact tccttatagt ccttacttgg
      841 aatatagcgc aatgcatttc gaacaagatg aacaatacag cgctgaacaa ttaccgatgg
      901 aaagatcgct tttgcgcctt cttcgaggcc agaaacaccg tccatcgaaa tgaaaaagac
      961 atcttcgaca ccacgtgctt tcagttcatc aaatacttgc atccagcgat ttttagattc
     1021 tgtttgattt aaccataatc ctaaaatctc cttatttcct ttgagatcat agccaagaat
     1081 ggtgtataca gcatattctt tggcttcata attttctcgt aaagtaacat acatacaatc
     1141 aacgaataga aaggcataac actttgctag ggggcgggct tgccattctt ccaattcagg
     1201 aaggacagcg tcagtgatat ctgaaatcat ttcatgggaa atatcaaagc cataaatagc
     1261 ttcgacggtt gcggcaatat ctcgttgact cattcctcgt gcatacatgg aaagaacctt
     1321 cccttcgatg tcggagacat ctcgttttct cttaggaatt aactctggtt caaaggaagc
     1381 ttcccggtct ctaggaacat caatagctac ttcaccaaaa ctggttttaa gcgttttagt
     1441 tccatagcca tttcgacggt tatcgtgttc cttaggctct ttagaatggg catcataacc
     1501 taaatgatta ttcaattctc cttgaagcat tttttcaaaa aggggcccaa acacatcttt
     1561 caaagcatct tgcatgtcat cgacagattc aggttgatag gcattcagaa tggattcagc
     1621 taacttttcg gcatcaggat ttcttttctt tctagccatc gtgtaatcac ctcttgatct
     1681 tattgtagaa aaagaaaaac cgcgaagcaa ccacctgcct aggattaatg gttacttaca
     1741 cggtttatat tacactctca tcattaaaaa agtatctttc gatattacta aacaatgaaa
     1801 ttatctattt gaaatttaaa tctgataata tattgagtgt tccaatgagg gctttgatcc
     1861 tattatccat atctttt
//
LOCUS       DAGCWU010000243         1817 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.253, whole genome shotgun sequence.
ACCESSION   DAGCWU010000243 DAGCWU010000000
VERSION     DAGCWU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.253"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            96..836
                     /locus_tag="MBZ71_002699"
     CDS             96..836
                     /locus_tag="MBZ71_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430592.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1127..1795)
                     /locus_tag="MBZ71_002700"
     CDS             complement(1127..1795)
                     /locus_tag="MBZ71_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4430593.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          678 a          318 c          330 g          491 t
ORIGIN      
        1 cgctattttc ttaaaataat cagcgcattg ctactgaaaa caatgaatct gtgctattat
       61 tgagaaaagt tccatttttg agaaggatgg tttgaatgaa agaaatcaga cgaaaacatt
      121 tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt aaccaacgac aagaagcgag
      181 tattatttga cggacaagaa gatcttttga ctgaacagaa tcagtccccg tttgaacaga
      241 tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat ccgtcgcaag cgtcgtgcaa
      301 caaatggatt gaagtcagct ccgcgtaagg aaaatttgga aaagcataaa ggaaaattac
      361 cggattatac cggccattac caatcagaaa tcacatcgac aggacgaaaa aaactttttg
      421 gtgattcctc tcgttctgtc agacaaagtg aaacaaggac agagtccaag catactacac
      481 gaaacgcatc tgttgacacg aaggtgcggg agcgttctta ttttgttcct aaatatgtac
      541 ccgcatcgat tatcccagac cctagagaaa gcaaaatttc tgaggatcat ttaatcgaat
      601 cgatgaaaaa aacggatgac tcttatctgt tgttcgatac agaaccgacg ccgtatcaag
      661 aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg aacaaaaacg ttagaggaaa
      721 caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg tatcttagaa cgctcattac
      781 aaggattgat cgatgatcaa ggaaactctt tgggagaaaa tagctatttt aaataagtga
      841 tatacaaatt tatattcggc tacagttgga aaggaaacgc tgcggcgttt ccttatttta
      901 tactctatcc gaaagtttat atcgtatcct caaatttttt tgagtttaag cgaatttttt
      961 ctcccaataa gtaagaaaat aatggcaaaa taaaaaaaca aactattttc gcagatcgtt
     1021 tgcaaaaata gtttattttt ttataaagaa gattttactg aataatatca ttttagttta
     1081 cataatgata tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag tgctctaact
     1141 gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca
     1201 aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc
     1261 taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac
     1321 caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt
     1381 gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa
     1441 tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta
     1501 tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc
     1561 ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa
     1621 gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag
     1681 taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga
     1741 ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta
     1801 gcctcttttc ttttgaa
//
LOCUS       DAGCWU010000244         1777 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.254, whole genome shotgun sequence.
ACCESSION   DAGCWU010000244 DAGCWU010000000
VERSION     DAGCWU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.254"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            200..427
                     /locus_tag="MBZ71_002701"
     CDS             200..427
                     /locus_tag="MBZ71_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBT4430594.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            550..876
                     /locus_tag="MBZ71_002702"
     CDS             550..876
                     /locus_tag="MBZ71_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBT4430595.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            944..1657
                     /locus_tag="MBZ71_002703"
     CDS             944..1657
                     /locus_tag="MBZ71_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBT4430596.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT          687 a          273 c          374 g          443 t
ORIGIN      
        1 ttaaacaaag tagttgacac ttccgctact tagtattagt acgaaagtat acttcatgat
       61 actaagtaga taagaagtga aaagcggaac ccacgatcgt caaaatgcta actgctttgc
      121 ttacaccaga taaagggaag agcaatatcg cttattttag gcgtggagaa gcaagcaaat
      181 ccctacgatc acaaaaaaaa tgctgaccca gcagttaaga gagttagaac aagatgaaag
      241 tatccatcga aaagcctata accaagtccc gcctaaagta gagtactcgc tcaccgaaga
      301 aggaaaatca ttaagagaga ttctagtagc gttgtctgtt tgggaagaag aacgtatcga
      361 aaaacaaaaa gcctcaaggg aagaagacac gctactgcat acacatggtg gctacctaaa
      421 ttattgattt tctatgacat ttattttaaa taaaagaaaa caggagtttt tttaaaagca
      481 tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaagtg attggaagaa
      541 ggcaagcaaa tgaatagtat ggaaaaagtc aaccagttta gagatgaacg taattggcga
      601 caatttcata acgaaaaaga tttggctatc tctatttctt tggaagcatc tgaattgtta
      661 gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga acgaatcaaa
      721 gaagaactgg cagatgtatt gatctattca tacatgatgg cagacaacct tcacctggat
      781 ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc tgtagaaaaa
      841 agcgcaggtg tacgaaaaaa atacacagag ttgtagaaat gtgtcattcg gctgttggaa
      901 agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa aaaaaggaaa
      961 agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag gactagcttt
     1021 gattttcaat gaacagatca aggattattt tgtcagagag acaggagata aatatgctat
     1081 agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca gttttgactt
     1141 tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcaaatga gaggaacaga
     1201 tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac cggttttcaa
     1261 aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag atcaagaaat
     1321 gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat tgctgtttac
     1381 accgctagaa aatttagaga tgggtgccaa gatctatttg acagatttag aaaatgtgta
     1441 tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat tactgaatga
     1501 agtcgaggga aaagaaattg tcacactaat tacttgtggc gacatggacg ccgtcacgcg
     1561 actagttgta caaggagaac tggaaagtgt tacatcgata aaagatgcga cagatgatat
     1621 gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat aagtcgttac
     1681 agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc tgaaaaaaag
     1741 agaaacgcaa agaggtgcat ctctgatttg gttgttt
//
LOCUS       DAGCWU010000245         1740 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.255, whole genome shotgun sequence.
ACCESSION   DAGCWU010000245 DAGCWU010000000
VERSION     DAGCWU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.255"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            71..1702
                     /locus_tag="MBZ71_002704"
     CDS             71..1702
                     /locus_tag="MBZ71_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBT4430597.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT          496 a          309 c          390 g          545 t
ORIGIN      
        1 tttatctcat gaaatatgat attctaatga atggtatttt agaaaatttc atgaaaaagg
       61 agttagatag gtgttcaaaa attatgtttc ctcactaaaa aaagaatttt caggttatga
      121 tctttcaaga tttcagaaag atcttttggc tgggatcaca gtagctgctg tagcattgcc
      181 tttagccttg gcttttgggg tatcaagcgg agctgatgcg gccgcaggtt tgattacggc
      241 tatcattgcc ggtatcgtta ttggcggttt atcaggcggt ttttatcaaa tatcaggtcc
      301 gacaggtgcg atggcagcta tcttgatgtc tattgcggca acaaagggaa tgcaaggtgt
      361 attgcttgct acttttttag cgggtctatt tttacttgca gcaggacttt tccgattagg
      421 aacactgact tcttttatcc cagcacccgt aattacgggg tttacttcag gtattgctat
      481 cattattgca ttaggtcaga tcgataattt atttggtgtt cactcggaag gtacaaacgt
      541 tattgaaaaa ctaagtagtt atcaaacgat aggatttccg ataaacacag cctctttgat
      601 catgggaggg ctagtcattt tagggatgtt tatctatcca aaaaaatggg cgaaacgaat
      661 gcctagttcg ctgctggcta tcattttggc aacagcagtt atgctattgt tccatttacc
      721 agtagcaact gtgggtaaga ttccacaaac attgatcagt agtaatcgtt tgaatatggg
      781 agattttagc ttttctgcct tgcaacaagt ggcagttcca gctattagca ttgctttatt
      841 aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc cgaatggcaa ataagccctt
      901 agatagcaat caagaattaa tggcgcaagg aattggaaat ctgttgcttc ctttctttgg
      961 cgggattcct gctactgcgg ccatcgcccg tacaagcgtg gccatcaaat ctggtgcaca
     1021 aactagatta gctggtatga tacatgcagt tgtcttactg ctctccatgc ttgtttttgc
     1081 gccagtgatg tcacagattc caatgccagc acttgcaggt gttttgattg tgacggcttg
     1141 gcgtatgaat gaatgggaaa cgatcaagga gctatttact aataaatatt ggtcagcagt
     1201 tctattgttt atccttacga tgatttgtac agttatcttt gatttaagta ttgctatcgt
     1261 gatcggggtg atcggcggct gtgtattttt tgttgtcaaa agcgctgcta tcacgatttc
     1321 attagaaacg atcgattggc aaagaatggg gctgccagaa acggaaaagc tgaataactg
     1381 gacagtagtg tatattagtg gtccactgtt ttttatgtct tcagaaaaac tcaaaaataa
     1441 attggaagaa ctaaatcatc acgatggtgt cattttctcc atgcgaggcg taccaagtat
     1501 tgatttgacg gcacgtgcag tttttgatga atttcaagaa aaagcagagc aaagaggaca
     1561 aacgattatc tatacttcct tgcagccaga agtagaaaaa caactttctc acttgtggga
     1621 acatacattc acgcatcaac atcaaacagt tgctcatgcg ttgacagctt tacatgcgaa
     1681 atatactgca attagccatt aaaacaatga aaaagaacag acaagactgt aaactgaaga
//
LOCUS       DAGCWU010000246         1727 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.256, whole genome shotgun sequence.
ACCESSION   DAGCWU010000246 DAGCWU010000000
VERSION     DAGCWU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.256"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1265
                     /locus_tag="MBZ71_002705"
     CDS             <1..1265
                     /locus_tag="MBZ71_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4430598.1"
                     /translation="ATDRASKETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKI
                     SQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKET
                     KFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADE
                     TVEVEIKGLFDGHNSGVVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKG
                     DKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVV
                     VSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADK
                     LGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFM
                     SLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            1395..>1727
                     /locus_tag="MBZ71_002706"
     CDS             1395..>1727
                     /locus_tag="MBZ71_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4430599.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          663 a          225 c          319 g          520 t
ORIGIN      
        1 atgctacgga cagagcttca aaagaaacat ttgctaatat aacaaatagt ttttctatgg
       61 agataaatag acaagtaaat ccaggaacac ctagaggtgg gggaaatgta aaaggtgaag
      121 atattaaaaa gatatctcaa acagatagta tagactccta tgtaaaaaga ataaacagtg
      181 ttgcagattt agttgattat gatattattg aaactcaaga gactcttgcg aatcaatcac
      241 ctgaaagagc aaagaatttt aaaagaacag ttatgttaac tggggttaac gactcaacaa
      301 aagaaacaaa atttgtatca gaagcatata aattagtaga aggaaagcat ttagaaaatg
      361 aagataaaaa taaaatctta atgcataaag atttagctga aaaaaataac cttaaagttg
      421 gagataagat aaaaataaaa tctaatttat ttgatgctga taatgagaaa ggtgcagatg
      481 aaacagtaga agtagaaatt aaaggtctat tcgatgggca taatagcggt gtagtaagtg
      541 cagcacaaga actatacgaa aatacattaa tcactgatat tcatacagct gctaaagttt
      601 atggtaatac cgaagataca gctgtatacc aagatgcaac attctttgta aaaggtgata
      661 agaatcttga tagtgtaata aaagatcttg ggaaattaga tataaattgg agagaatata
      721 atttgattaa aagttcatca aattaccctg cattacaaca atctatatca ggtatttact
      781 caatttcaaa taaattattt tttggctcat taatatttgc aggagttgta gtttcattat
      841 tattattctt atggatgaat gctagaaaga aagaaatagc agtattacta tcattaggta
      901 tatcaaaatt agaaattttt ggtcaattcc taattgagat ggtatttata tcaatccccg
      961 cattcgttgg ttcctatttc ttagctcaat atacagctga caagcttggg aataatatat
     1021 tgaataaggt tactggtgat atagctaaac aaatagctaa acaatctgca tctagccaat
     1081 taggtggtgg ggcagaagct gaaggattta acaagacatt atcaagttta gatatcaatg
     1141 tattacctaa attcatgatt tatgtagtta tatttatgag tttagtactt cttgtatcat
     1201 tgattatttc ttcattcaat atattaagaa ggaatccaaa agaattatta attgacaata
     1261 attaaaattt tcggtgcttt ttagcaccaa aaatgagagt gtaatataaa ccgtgtaagt
     1321 aaccatcaat cctaggcggg tggttgcttc acggttttta tttttctaaa ataagatcaa
     1381 gaggtgatta cacgatggct agaaagaaaa gaaatcctga tgctgagaag ttagctgaat
     1441 ccattttgaa tacttatcaa cccgaatctg tagaggatat gcaagatgtt ttaaaagatg
     1501 tgtttggtcc cttatttgaa aaaatgcttc aaggagaatt gaataaccat ttagggtatg
     1561 acgcccattc taaagagcca aagaaacatg acaatcgacg aaacggttat ggcaataaga
     1621 cgcttaaaac gagctttggt aaagtaaaca ttgatgttcc cagagaccgg gaggcttctt
     1681 ttgaacctga gttgattcct aatagaaaac gagatgtctc cgacatc
//
LOCUS       DAGCWU010000247         1723 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.257, whole genome shotgun sequence.
ACCESSION   DAGCWU010000247 DAGCWU010000000
VERSION     DAGCWU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.257"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            33..1517
                     /locus_tag="MBZ71_002707"
     CDS             33..1517
                     /locus_tag="MBZ71_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBT4430600.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDIPQNMMSAIVDSNRTRKDFIADRVLQKTGYYSYDDDN
                     TFDLSLEKEVIIGVYRLTMKSNSDNFRQSSIQGIMKRIKAKGATVIIYEPTLKDGSTF
                     FGSKVINDLEKFKSISHSIIANRYDSILNDVKEKVYTRDLFQRD"
BASE COUNT          657 a          291 c          277 g          498 t
ORIGIN      
        1 gttattatta gcaacaaaaa aaggagtgca tcatgaaaaa gttatctcaa aaacgtttag
       61 ctcaaatcat tattgattct agaaaatctc tgaaaatgac acaacaacaa cttgcagtag
      121 ctactggtat caaccgtgcc ttaattagtc gagtagaatc tctcgatttt atcccgtcaa
      181 tacaacaatt agaatcatta gcagaagttc taaattttga accaacagat ctatttataa
      241 atgaatcaaa taaatcaaac agaacaaaac atcagtcgaa gaatattgct gtagccggta
      301 ctggttacgt aggtctttcc attgctactc ttcttgccca gcacaatcag gtaacagcag
      361 tagacattct tcctgaaaaa gtaaaacaaa ttaacaataa gaaatctcca atacaagacg
      421 attacattga aaaatatcta gctgaaaaaa atcttaattt aattgcaacg actgatggtg
      481 cagccgccta tagagaagcc gattttgttg tcattgctgc tccaacaaat tatgatagta
      541 aacaaaatta ttttgatact tctgctgtag agtcagtaat caaattggtg ttagaagtca
      601 atccaaaggc aataatggtc atcaagtcga cggtacccgt aggctatacc gaaagtattc
      661 gtaaaaaata caatactaaa aatattctat tcagcccaga atttctcaga gaatctaagg
      721 cattatatga caatctatat ccttcgcgaa ttattgtcgg cacggaccaa actgacaaaa
      781 ctttacgaga agcctctcac acttttgcag atttactcca agaaggagca atcaaaaaag
      841 atatcgaaac tcttttcatg ggattcacag aagctgaagc agttaaatta ttcgctaata
      901 cttaccttgc acttcgagta tcttacttca atgagctgga tacttatgca gaaatgaaag
      961 gactaaatac acaagaaatc attcacgggg tttgcttaga tccaagaatt ggcacacatt
     1021 ataataatcc atcttttgga tatggaggct attgtttacc gaaagataca aaacaacttc
     1081 taactaacta taacgatata cctcaaaata tgatgagtgc aattgtcgat agtaaccgaa
     1141 caagaaaaga ctttatagca gaccgcgtgc ttcaaaaaac tggatattat tcttacgatg
     1201 atgacaatac tttcgatttg tctctagaaa aagaagtgat tattggagtc tataggctta
     1261 caatgaaaag taattcggat aacttccgtc aatcttctat tcaaggtatt atgaaaagaa
     1321 tcaaggcaaa gggagccact gtgataattt acgagccaac acttaaagat ggtagtacat
     1381 tttttggttc aaaagtaata aatgatttag aaaaatttaa aagcataagt catagtatta
     1441 ttgctaatcg atatgatagt attcttaatg atgtaaaaga aaaagtatac actagagatt
     1501 tatttcaacg ggattaaacg aatgattaga tacattaaaa taatttacaa taaacagcaa
     1561 gactaatttt tatcctttca tttagaccca ttaattaagc gctaggagga attacgtctc
     1621 tctttagaaa agatttttaa taaaagatta ccaactttaa gaaatctaat tatcaaaatt
     1681 tttacaacgc aaggggagtg ggacaatagt cagaaaaaaa tct
//
LOCUS       DAGCWU010000248         1709 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.258, whole genome shotgun sequence.
ACCESSION   DAGCWU010000248 DAGCWU010000000
VERSION     DAGCWU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.258"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            78..452
                     /locus_tag="MBZ71_002708"
     CDS             78..452
                     /locus_tag="MBZ71_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBT4430601.1"
                     /translation="MENIFDLNKGLSRRKYWYYQCILFVFGLGIETFSMFVYKKSLIE
                     NNYLKYFVINGPFDFYNFLRIVFFIMIIIGFLFQARRFVDIGWSKFVALLSLLPLVGI
                     ITTGICLFKKGKDNKTDSSQVS"
     gene            951..1610
                     /locus_tag="MBZ71_002709"
     CDS             951..1610
                     /locus_tag="MBZ71_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfX family protein"
                     /protein_id="HBT4430602.1"
                     /translation="MEENNIESKDNLFKEIGEKMYPLFFNRNFGLATKSEIESMLFYS
                     YLKHKINSNSDYSDRTLSRELGISEVKVRNLKRTCYARYEDAIDFPKLLTSLSKEGSN
                     LMFFKIYTDNNDVLCRISISEVVYYDELRTQLNNSGILFKKNPGSNYVELSMADAIIF
                     FDQDSISKESLDLKNLIDKMKQLNFKTNTKMDIDKILEGTKAGRMKNVVEAMMKLSNS
                     I"
BASE COUNT          608 a          190 c          307 g          604 t
ORIGIN      
        1 tttatgtatt tgccaattac aatggtattg taaataaata cataaagtag cattagtagg
       61 ttttggggga tatagatttg gaaaatattt ttgatttaaa caagggatta tcaagaagaa
      121 aatattggta ttatcagtgt atactgtttg tgtttggatt agggatagaa actttttcaa
      181 tgtttgtata caaaaagtct ctgattgaaa acaattattt aaaatatttc gtgataaatg
      241 gtccatttga tttttataat tttcttagga tcgtgttctt tataatgatt attataggtt
      301 ttctgttcca agcaagaagg tttgttgata ttgggtggtc gaaatttgta gctttactaa
      361 gtctgttacc gttagttggt attatcacaa cagggatttg tttatttaaa aagggaaaag
      421 acaataaaac agatagcagt caagtttctt gattgctatc tgttttattt attaagagta
      481 ttgtctatta aatgaatgac aagaacttgc attgtaagca gcttgaatcg cagctgacca
      541 tgagagttta tggaaggcta ctgcagacca aattcctttc ataatacaag caccttgttt
      601 tgctttagct tgttgatatt tattccataa atgtttaggg actttacttt ttgcaaagtt
      661 gtcctaataa ccatcgtcac tttgggcata tgcagtagta aagtttacta acggataata
      721 catgataaat ttaaaaggta gggtactttt tcgtacaaga atgattttgt aagtatgaaa
      781 caagctagtt atatttcact agctattcaa tgaaataagt ttttaaagta gattgttgat
      841 agatatatta aaattttttt tcatgtaaga gtatgctaaa attgatgtta agaatggttt
      901 tccctagtaa taggtaacat caaaataaag gaaggtgtaa ttctatacct atggaagaaa
      961 ataatataga aagcaaagac aatctgttta aagagattgg ggaaaaaatg tatccgcttt
     1021 tttttaatcg taattttgga ttagcaacaa aaagtgaaat tgaaagcatg ctcttttact
     1081 cgtatctaaa acataagata aatagtaata gtgattactc agatagaacc ttgtctagag
     1141 agctaggaat atcggaagtt aaagtaagga atcttaagag aacatgttat gcaagatatg
     1201 aagatgcaat tgattttcct aaattactaa cctcgctgtc caaagaaggc tcaaatctaa
     1261 tgttttttaa aatctatact gacaataatg atgtattatg taggatatct atttccgagg
     1321 tagtttatta tgatgaactt cgaacacaat taaacaatag tggtattctt tttaaaaaaa
     1381 atccaggatc aaattatgta gagttatcaa tggcagatgc aataattttc tttgatcagg
     1441 attctatcag caaagaaagc ttagatttga aaaatttaat agataagatg aaacagttga
     1501 attttaaaac taatactaaa atggatattg ataaaatatt agaaggaact aaagctggta
     1561 gaatgaaaaa tgtcgttgag gctatgatga agctttccaa ctcaatataa acaaagaggt
     1621 cagaaacttc ctaagttttt tgacctattg ctttttcgtg tgtaactttt ttatttaaat
     1681 ggcataagtt tttatcgatg aatggcaaa
//
LOCUS       DAGCWU010000249         1691 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.259, whole genome shotgun sequence.
ACCESSION   DAGCWU010000249 DAGCWU010000000
VERSION     DAGCWU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.259"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(473..610)
                     /locus_tag="MBZ71_002710"
     CDS             complement(473..610)
                     /locus_tag="MBZ71_002710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430603.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(705..1283)
                     /locus_tag="MBZ71_002711"
     CDS             complement(705..1283)
                     /locus_tag="MBZ71_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBT4430604.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            1452..1649
                     /locus_tag="MBZ71_002712"
     CDS             1452..1649
                     /locus_tag="MBZ71_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430605.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
BASE COUNT          550 a          311 c          254 g          576 t
ORIGIN      
        1 cttatttagg ctcccttctc cttgattctg ccagtatatt ctgcctgatc ctactttttg
       61 aaggcatttc aaaggaaaaa aacagtagtt tttagaagta gtagagaaat ctgagacacg
      121 gtattttctg tgtcgttatt ttttaagtat aaaataacaa aagcgtaaaa tgagatatgt
      181 tattttttta ttccaaaaac cacaccgttt tatactgttt gaaaacacgt taatatttat
      241 tcatttctgt tgtatatctc ctatcaaaag aaatctatac gcccttcttt gactaatttt
      301 ttatttttat ctcttacgaa gttatccact gtggaaaaac taactatcta tttttcgata
      361 cacttttcta aaaattaaat tttccacaaa taaaaacacg tttacaacag aatcatactg
      421 ctgtaaacat gcttttctta aatagataga acatcagatt acataaagat gtttattttt
      481 ttattgctgt gaataatgtc aaaaatccta cgacaccaac tactgtccaa gtcaaatgac
      541 cttgatccaa ataaataagc atcgtgacaa aactagtaat cagtcctaca tgaattcttt
      601 tttgatccat ctgcatccct ctttgaatac tagtatcact cggatatttg atcgtttatt
      661 tattttttct atatttttct cttaaatttt aaacacggat cattttaccg atattgtgat
      721 tcgatggtac gacttgggat gatccaagca gctagaatca cgatgtttgc gatcagcacg
      781 gctactggcg ctactaaata ccctaagaca attcctaaca cattcaaacc aatcgataga
      841 tacgatttct tgtaattatt caacatcgcc ttcaatgctt tctcgtggcc tttatttgct
      901 ttgacaagtt cttttgccaa gatataatac gcaatattag cggtcaaggt aacaaaacca
      961 taagtcattt ctggagccag gctattgatg tactcactga tccatgaagt tgcaaacgga
     1021 aaaagcgaaa gcgcaaaaac aaagaaactg ttcgcccata agacccgtcc atttatctta
     1081 tgtacaactt gaaataaatg atgatgattg ttccaataaa tcgctaaaat gataaaactg
     1141 acaatataga taaaaaatcg gtaacctaat gcagcgagcg cctcccacgt gtctccgtta
     1201 ggttcatgaa gttctaaaac taaaatagtc atgataatcg cgattacagc atctgtaaat
     1261 gcttcaagac gatttttagg catgatattc tcctcctttt ccgctctatt ttagatgata
     1321 aaccagagaa aaagcaattg tttgatcatt taagtagatg aagtaaaaat cagtcttcag
     1381 ttggaggtgt ttttaaacaa tccctgttat actttttcta tagtaaagaa tgaacaagag
     1441 gagggttttg tatgcgtcaa aaaaatatgt tcttaaatat cttcatggtt cttttagtgc
     1501 taattgcagc tagcttcgtc atcagtcttt ttgtcagtgt cctaagcggg cttttatggt
     1561 ttgcggtcaa agttctaatc ccaatcgcac ttatcatctg gcttgttcgc tggatcaaag
     1621 gaaatacaca tagaaaaaac tattattaat gtatgatacc attcacttca ttatgatata
     1681 acttactaaa a
//
LOCUS       DAGCWU010000250         1630 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.260, whole genome shotgun sequence.
ACCESSION   DAGCWU010000250 DAGCWU010000000
VERSION     DAGCWU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.260"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            61..657
                     /locus_tag="MBZ71_002713"
     CDS             61..657
                     /locus_tag="MBZ71_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBT4430606.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(715..1197)
                     /locus_tag="MBZ71_002714"
     CDS             complement(715..1197)
                     /locus_tag="MBZ71_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430607.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1303..1500)
                     /locus_tag="MBZ71_002715"
     CDS             complement(1303..1500)
                     /locus_tag="MBZ71_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430608.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          547 a          338 c          254 g          491 t
ORIGIN      
        1 tacttcattt cgtaatcaat taaaaaaatg caaaacgcaa atcaaaggag aaattttatt
       61 atgacaactt ttactgacac tttgaaaaac cgccgttcaa tctatcatct aggacgtaat
      121 gtttcacttt ctaatgaaga attaacaact ttgatcaaag aagcaattaa agaaagccca
      181 actgctttca atgctcaatc aacacgcgca gttatccttt tcggagatgc acatgaaaaa
      241 ctttgggaaa tcactgaaga agcacttcgc ccattgactc cagcagaagc tttcccaaat
      301 acacaaaaca aattagctgg tttcaaaaat ggttatggta cagtattatt cttcaaagat
      361 actgacgtgg taaaaggtct acaagaacaa tttgaattat atgcagacaa cttcccagat
      421 tggtcagaac aatcaaacgg aatcgcaaca gctaatactt gggtagcatt agttgataaa
      481 ggattgggcg caaacttgca acattacaac ccagttattg acgaagcagt tgcaaaagaa
      541 tggaatatcc cttcaaactg gaaactacgt tcgcaattag tattcggttc accagaaaca
      601 ccggctgggg aaaaagaata catgaacgat gccgatcgct tccgcgtatt tggataattt
      661 tatttgaaat aaaaaacaga ggttgtgaca aacatcttgt cgcaacctct gtttttattc
      721 tggatcaatc ataatatcaa tctctgcctc atcgtttaag cgttgggaca aaaatggaac
      781 taattcttct aatgatgcag aaaatttcag catcggtcct cgccatccgt ttcggaaaaa
      841 attcattttt acgattttag aaccttttgg ctgatataat acaattttat tgatctcaga
      901 aaaaggaaca ccttttttgt caaagctatt caaaacaagt ccttcttctg tgattcctct
      961 actgtccaac aaaaaactaa taaaaatcat tgctgacaag atcccttttg ccaattcatc
     1021 tgctgtagga tgactgatga gtgtatacca aatgactagt gacactaaga agggtatcac
     1081 caagtagacc catcgatttc gcacagtccg ataaataatt tcttttcgaa ccagccaaag
     1141 aaataaaaac atcacaacaa cgaataaaaa caatagaacc gtaaagaatg ttaccatgac
     1201 aactcgcctc gctttttctt ctccagaaaa taggaaaaga atactgattt accttctcaa
     1261 agaaattgga cttgcagcac gccgcacgtc caattgtttt ctttattcaa atgtatcata
     1321 ttttgcaaca accatgcttg ctttttcatc taataattct tggtcctctg cagcacattg
     1381 ggcaactttg accacggcgc agtttgtcat ttttgataca acttcaccaa tgaccgtttt
     1441 tgtaaaacca attggtttgc atgcatattg ttctcctaaa ttaatcattt cttccgtcat
     1501 caaaatcctc ctaaactgct ggaacttttc tccacattac cactgctatt tctttggaga
     1561 tttaactcat tgatttttat ttacagttaa aaatcaacct tacaggcatt atagcgtact
     1621 gttttcgttt
//
LOCUS       DAGCWU010000251         1616 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.261, whole genome shotgun sequence.
ACCESSION   DAGCWU010000251 DAGCWU010000000
VERSION     DAGCWU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.261"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            91..705
                     /locus_tag="MBZ71_002716"
     CDS             91..705
                     /locus_tag="MBZ71_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4430609.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(729..1079)
                     /locus_tag="MBZ71_002717"
     CDS             complement(729..1079)
                     /locus_tag="MBZ71_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4430610.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1073..1336)
                     /locus_tag="MBZ71_002718"
     CDS             complement(1073..1336)
                     /locus_tag="MBZ71_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBT4430611.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          548 a          284 c          252 g          532 t
ORIGIN      
        1 aactagacac gaccgcgaca ttgaaaaatc tggttttctc gtgtatgatt agggtgtttt
       61 ttaaatagtt gcagaaagta ggtaaaaatt ttgagagttg catacatacg tgtctcatcc
      121 atcgatcaaa atgaacaacg gcagattgaa gaaatgaaaa aattcggtgc agagagaatt
      181 ttcattgaga aacaatctgg cgcaacgatt actcatcgac ctgtttttca agaagcactg
      241 gatttcgtcc gagatcaaga tatttttatt gtggaagcta ttgatcgact tggaagaaat
      301 tacgacgaga tcatcgcttc agtcaattac ttgaagaaga aaaatgtgca gctgatcatc
      361 acaagtctac cgatcatggc cgaagcaatt ggcaatcctc tcctggataa attcattaaa
      421 gatttgatca ttcaaatttt ggccatgatc gctgaacaag aacgtaccga atctaaacga
      481 cgtcaggcac aaggcattaa gattgcaaaa gcgaacggtg tttataaagg acgtcctaag
      541 ctatacagtg ccaatgcaaa agatccccaa cgtcgtcttg tttataaaaa catcgttgag
      601 gatctaaaaa aaggtgttgc gatcgctaag attgcaaaag actacaatgt tacgagacaa
      661 accgtatata gaataaaaaa agacagcatg gtcaatcacg aatagttgat catgctgctg
      721 tttctatttt atttgcttac tttttgaaaa actgttctaa ctttcatcaa taactcctca
      781 actaaactat catgcgcttt ttccaaaaaa atacactctc tggcttcata atcaattgtc
      841 gttagttgat ctaataatac ttttcctgtt gtcttcattt catattctga gtttatattt
      901 acatatagcg gatagtttct agttgtacta gaaattggag caactgtaac aacgttacta
      961 taatccgcaa taatatcatg acttaaaaca atataaggtc ttttacctgt ctgttcgtgt
     1021 ccagatcgag gtgctgtatt caataataga atatcgcctt gtctaggttt tctcaccatt
     1081 tttcattacc tactgcttca cctaaatttt gaatcgtcac atgatttctt tcatcagtgt
     1141 aattttcaaa taactgttca attgttaatt cattttctat attagccgct tcgataatta
     1201 gccgcttctg accgtttact tcttcttgta ttatcttcac tttgtcttct gtatgaattc
     1261 caagatattc cataatatac tttggcaatc taaggccatt actatttccc cattttttta
     1321 taggtaacat ttccatagta gacacctctt tctgtatata catagtataa catacataag
     1381 aaaaaacgta tatacttaat tatccttttc tattaaatta gattaacatt ccatgaatcc
     1441 ataaactatg ttaacattta tatgaaaata gtcgaaagga aatcataata tgtttaagaa
     1501 aacaaaatct ttagaatcag gtaagaaaaa aattgatcta aaaataatct cttctggata
     1561 ctttaaagat ttcatagtta ttgatacaaa atggaataaa aaggacatag aaaata
//
LOCUS       DAGCWU010000252         1614 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.262, whole genome shotgun sequence.
ACCESSION   DAGCWU010000252 DAGCWU010000000
VERSION     DAGCWU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.262"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..462)
                     /locus_tag="MBZ71_002719"
     CDS             complement(<1..462)
                     /locus_tag="MBZ71_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4430612.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGC"
     gene            complement(473..1141)
                     /locus_tag="MBZ71_002720"
     CDS             complement(473..1141)
                     /locus_tag="MBZ71_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBT4430613.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1272..>1614)
                     /locus_tag="MBZ71_002721"
     CDS             complement(1272..>1614)
                     /locus_tag="MBZ71_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4430614.1"
                     /translation="FNELDPFINYIFENKRIFDLVLFQSAGTKYQDIVEKITSFVTKK
                     TVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQ
                     FSVPGWIELLS"
BASE COUNT          591 a          268 c          211 g          544 t
ORIGIN      
        1 acatccaata gaaattttct gccgttcacc acttgatagc gaaaaaatgt ttttatctaa
       61 tatatttgaa agatctaata attcagctaa ttcattgatt cttttatcca tatgctcttt
      121 tgtaaatcca aagttttcca tcgaaaatac taactcactc gttgtatttg ttgtaaagaa
      181 ttgtcctcta ggatcttgac ttacgtagcc aatatttttt gatatacttt cttgtgttaa
      241 ggattgtagc gagttatttt caaataaaat ttccccaacc aattttccgt cataaagtct
      301 tggaataagt ccattcatta actttaatag tgtggatttc ccactcccag aagaaccagt
      361 aatcaatgta agactacctt tttttattga aaaattaagg tcaacaagcg cttgttcctt
      421 aacatcctta tatgaaaaag aaacattgtt aaattcaaac aattctttca cctcaaatca
      481 aaatacttaa gaaaaaaata gtaacggaaa acaggatgat atagtctaat aagctcatgt
      541 ttgttttata gtaagaactt cttttcacag gagattctat cccctttgtt attgcattag
      601 cggtcatttc ttcagcaatc atagttgcac gtataagaac gggaactaac agaaattcca
      661 aatattgcag aggctgaaaa aaagacaagc gaatgtttct catttttaaa gattctttaa
      721 tcattcttat ttcagaacga taagtcggaa agaatctgat cactactgta atacttaaca
      781 ttattttttt cggtaaatgt attttttcta aagaggataa taattcattg ctagtataga
      841 cactaaccaa caaccatgag atcattacga ccggcaacat tcgaaataca atatataagg
      901 atgtcccaaa aaatatagta aaaaactcta tcttgatagc atcaataatt ttcattacta
      961 aaaacagaac tgaataaatc aagatgaaat aaatacatgt ctttatttta cgaaacatca
     1021 atactaaaac tgtagcaata atgaaacacc ccacttgaaa tatatttccc tttcctaaaa
     1081 aaataatgac cggaaaaaaa atatttaaag taatcaaggt taacggattg atttttgaca
     1141 ctctttcacc tccaaaaaat aagatcgcac tgttatctta agctgataaa aatgcaccgt
     1201 tattcagata acagtgcgat cttatcaaaa agcataactg agaacaattc tcaatgcaag
     1261 ctttttttta attaactcag taattcaatc cacccaggaa cagaaaattg atacaatctt
     1321 ctggttaata gcatagtctt ttcttttgaa tagctatgct tcaatatatc atagaatgta
     1381 gcgtagaatg agtaaatgta gaagtggatt tcttcttcat taaagactaa attttgattg
     1441 atcattccga tagtctttaa cttatctaga caacagactg tcttttttgt cacaaatgat
     1501 gtaatttttt caacaatatc ttgatatttg gttccagctg attggaataa aaccaaatcg
     1561 aatatacgtt tattctcaaa aatataatta ataaatggat ctaattcatt gaag
//
LOCUS       DAGCWU010000253         1581 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.263, whole genome shotgun sequence.
ACCESSION   DAGCWU010000253 DAGCWU010000000
VERSION     DAGCWU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.263"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            133..507
                     /locus_tag="MBZ71_002722"
     CDS             133..507
                     /locus_tag="MBZ71_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4430615.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="MBZ71_002723"
     CDS             complement(621..1250)
                     /locus_tag="MBZ71_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBT4430616.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="MBZ71_002724"
     tRNA            complement(1384..1456)
                     /locus_tag="MBZ71_002724"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
BASE COUNT          548 a          234 c          273 g          526 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg c
//
LOCUS       DAGCWU010000254         1574 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.264, whole genome shotgun sequence.
ACCESSION   DAGCWU010000254 DAGCWU010000000
VERSION     DAGCWU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.264"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            110..>1574
                     /locus_tag="MBZ71_002725"
     CDS             110..>1574
                     /locus_tag="MBZ71_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4430617.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLT"
BASE COUNT          572 a          219 c          287 g          496 t
ORIGIN      
        1 actgatgaaa aaacaagcat aatccatcat aaaatcagta taggtgaaaa gatgaacttt
       61 catctgcata tcaagtaatt ggtatgaggc attttttgag gtgtaggata tgaagttgga
      121 ttaccttata gaagaggaag atagagaaaa actgctgcta gctagatatt tagaatttga
      181 gaaaaagaat tatgtagtgc tgcctaagtt atatgaaaca ttaggtttgt ctaaatttaa
      241 aacaagaaat tatatcatag aattaaataa agaatttact gaattcaaaa caaagccaga
      301 gatccaaatt tcagaagaag cagagatcca tgtcgaacgt ctgaatttga cggatatcaa
      361 aaatttaaga agaatctact ttcaacgatc aaaaattgct cgactgttaa cggagatcgt
      421 ggattcaaaa gtaacgatcg aaaaatttgc agagaatcat tttttaagca tatcaagagc
      481 ctatgtaaaa agaagagaat tagtctcttt tcttgaacag catcaaataa agttaaagaa
      541 aaacatatta gttggggaag aaataaacat acggaacgta ttatttgggg tatatttcgg
      601 ggtctttaat ggtttttcta tgccatttag agaaagcgtc atgaatgaag caaagagtat
      661 cgtgcaatat tttgaatatc tgttcaattt aaaactgtct gcaacaaaac gaatcaaatt
      721 caatttgcta accactatta gtttgttgag ggcctatggg aatcaaccaa tcggaaaacg
      781 tttcttttca gatgaatact ttaagagctc agaaggaatg aagatcatca aggatttatc
      841 acatatttct tttcttcata tccctaaaga gcagatcatt gatgagattt cttatatcat
      901 gttgtttttg atcacggaag aagaatttga attagatggc gcatttggac ttgcggaagc
      961 tcattttcat gatatccaca tgattagtca aaaaatgtca gtggaattga ttgatcaatt
     1021 accattttcg attgaaatct ctgaagaaga aagactatat atacgaaaag aacttcgtat
     1081 gggtatgatg catgtcaatc gaaaaaattg gatattcgac tttgagatga ctagctttac
     1141 gtctaaaaag cagctacagt ttttcttcga gacgtatcca atattcagta cagcaatcca
     1201 aaaggtcata caagagaata aacgctattt cttgggaaaa gatgaacgtt tcatgattcg
     1261 cttattttat gattatctct ttttattggt cggtatttgt ccggtatcat attttgaagc
     1321 accgattcat gtctgtattg atttttcgca aggatctgct tataccgatt acattatttc
     1381 tcaagtgaaa ggatttaaaa attacaatat tgtgattgag agtcgaatca cttctcgtac
     1441 gaatatttat ttatctgatt gtttacttga aaaatttaat aaagaacaga taatttggaa
     1501 aaatccaccg actccagatg actgggaata ttttggaaat accatcatca gagaaaaaaa
     1561 tagacattta actg
//
LOCUS       DAGCWU010000255         1524 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.265, whole genome shotgun sequence.
ACCESSION   DAGCWU010000255 DAGCWU010000000
VERSION     DAGCWU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.265"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            86..496
                     /gene="spx"
                     /locus_tag="MBZ71_002726"
     CDS             86..496
                     /gene="spx"
                     /locus_tag="MBZ71_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBT4430618.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     TLAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            510..866
                     /locus_tag="MBZ71_002727"
     CDS             510..866
                     /locus_tag="MBZ71_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4430619.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVEKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          562 a          227 c          280 g          455 t
ORIGIN      
        1 ttttttttat attttattag ctaataattg gtattttaaa agaaattact gtagaattgg
       61 attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta gctgtacttc
      121 ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag agaagaatat
      181 ttttgccgaa ccgttaacag aaagtgagat caaaaagacc ttggcagcga ctgaaggagg
      241 agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca tagattttaa
      301 cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat tattgagacg
      361 acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg agatccggtc
      421 atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac aaaacaataa
      481 catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga tcagcgcact
      541 gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct tatcaatttg
      601 ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc agttttgaag
      661 aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac attgcgattg
      721 acctttcctg tgtagaaaaa aaacaactga tttttttatg cacacaacat accaacctcc
      781 tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat gagcagttga
      841 tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca ctgagcattc
      901 catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct ttttttgact
      961 ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa tctaggtggg
     1021 gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc atttctgttg
     1081 ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag ttgttgacat
     1141 gttagaaatg acatgataag ataactgagt cgttaaaaaa ggaccgcttg cgaagcttga
     1201 ttctatcgaa aaaacttcaa aaaagttctt gacaaataac aaacgacaag ttatgataaa
     1261 tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa gagttgacaa
     1321 ttacttgtct ctctgatatg atataaaagt tgctgcggca gccaaaaacg aatcgggaaa
     1381 cactcctgat gaagtagacc tttgaaaaat gaacaaagta agacaaacca aatgtgtagg
     1441 gcgtcttgat tcaattcaag acaacaaaca tttttaacaa gcaagcaata tgctagcaaa
     1501 caaattgagc ttaacaatcg caag
//
LOCUS       DAGCWU010000256         1523 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.266, whole genome shotgun sequence.
ACCESSION   DAGCWU010000256 DAGCWU010000000
VERSION     DAGCWU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.266"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            12..1478
                     /locus_tag="MBZ71_002728"
     CDS             12..1478
                     /locus_tag="MBZ71_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBT4430620.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          324 g          493 t
ORIGIN      
        1 gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg aagggattgt
       61 caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg cgtgcattac
      121 ttatctatta tatgtacgat acagttgcca acgggggact tggattacca cgagcaacag
      181 cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc gtcggcggat
      241 gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga gttttcatta
      301 tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt gtttcgatgg
      361 cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg gtcggacatc
      421 tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac atgggtgtca
      481 acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa tataactatc
      541 atttagggtt ctctttagct gctattggga tgttctttgg attattacaa tacatcatcc
      601 aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg acaactgaag
      661 aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct gcagtattcg
      721 gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca atcagtgtat
      781 tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa gatgtaactg
      841 ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca atcattttct
      901 ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa cgtacacaat
      961 ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat ccaattttca
     1021 tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa cgtcaaccat
     1081 ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac ttgctattga
     1141 tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg ttgttgggaa
     1201 gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg tctgtgacaa
     1261 caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc ttagctgatg
     1321 cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga acagaatctg
     1381 cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta tttactgtga
     1441 aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata aaaacaggaa
     1501 ttgcttattt gcagcagttc ctg
//
LOCUS       DAGCWU010000257         1501 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.267, whole genome shotgun sequence.
ACCESSION   DAGCWU010000257 DAGCWU010000000
VERSION     DAGCWU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.267"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            5..403
                     /locus_tag="MBZ71_002729"
     CDS             5..403
                     /locus_tag="MBZ71_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBT4430621.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="MBZ71_002730"
     CDS             387..1082
                     /locus_tag="MBZ71_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBT4430622.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          568 a          216 c          302 g          415 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 a
//
LOCUS       DAGCWU010000258         1478 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.268, whole genome shotgun sequence.
ACCESSION   DAGCWU010000258 DAGCWU010000000
VERSION     DAGCWU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.268"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..703
                     /locus_tag="MBZ71_002731"
     CDS             <1..703
                     /locus_tag="MBZ71_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBT4430623.1"
                     /translation="LLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIP
                     SVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKV
                     RDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRES
                     FKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSP
                     KQYQLGLVDNFNNIS"
     gene            848..1153
                     /locus_tag="MBZ71_002732"
     CDS             848..1153
                     /locus_tag="MBZ71_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430624.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
BASE COUNT          545 a          170 c          258 g          505 t
ORIGIN      
        1 tctgttatca caaaattacg atccagaaat atatctgttt ctttatgaaa ataaaatact
       61 ggaatatgtt aaaaatggag atattcgaaa tttagaaaac atggttttta atctgagtaa
      121 tggaataatt ccaagtgtta gtggagatac gattcggtct gaaaaaaatt attctatcat
      181 tgtttttgaa aagttagcac aaacgtctat aacattagga atggatataa tagaagcgta
      241 tcaaagtaga gatgctttga tacaagaaaa cgaattagct gtatcgttac ctgaggtctt
      301 aaaagtgagg gattctggga ttgtttatta tactaaggaa atcggaaaaa ctaagataga
      361 gcatctttct cccttaattt cttctgtcgt tcaattcatc ggcttaaata tctataaaag
      421 aattactgta aaggaaatag ctaattattt ttctgtaagt gaaactaaat taagagaaag
      481 ctttaagaat gaaatgcata ttacgattta taattatatc tctaagcgaa aaatttctac
      541 ggctaaaata atgttgaaat caaaccatac tatcagtgaa gtttctttag gtttaggatt
      601 ttcagattct tcacactttt cgagagtatt caaaaagtac gcaggtgtat ctcctaagca
      661 atatcaatta ggtttagtgg ataatttcaa caacattagc tgagaggttt tattttatta
      721 tgagactata tcatttacgt agtttggagg agttgaggaa attggatttt aacaaaaaat
      781 tttttgggaa atttctgatt tgagttgttt cgtagaatct gagcgtctaa gtaatggtaa
      841 taatagtgtg ttaaatgaat atgacaaagt agtaattaat aaagtaaaaa gtttatgtga
      901 agaagctaga gaatctatca gtcatattaa tggtagcaat tttaagcaaa cattatttaa
      961 tctgattaag ctagatgcta aaatatcaag ttatttattc tttttaatac atgatgaatt
     1021 tatggattat caaaaattgg atcagttaat tgaaagagaa agctgggaag attattacgt
     1081 gggtctaact ttttcagaaa agttaaacaa ctacaagtta atagattata agtatttgag
     1141 tgaaagcaat taattattca tgtagagtac tagacacttt aaatatggac aattcaatta
     1201 taaagaaaaa gaatcaataa tttaatgagg ctgtgacaaa tgtcattttt tgtttcgcta
     1261 aaaacgagtg aaaaaatgtt gataactcca atcattcgtt taagattcta ggaatcattc
     1321 aggaatgctg tgaaaccgat actttataat tataaagaag gtgatttcaa attccgaaga
     1381 aggaatttga agtcaccttt ttacttatgt cacagtctca ttcagtaatg cgggataagg
     1441 ctccttattc cgctatttga atactctgaa gtttaatc
//
LOCUS       DAGCWU010000259         1463 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.269, whole genome shotgun sequence.
ACCESSION   DAGCWU010000259 DAGCWU010000000
VERSION     DAGCWU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.269"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(52..124)
                     /locus_tag="MBZ71_002733"
     tRNA            complement(52..124)
                     /locus_tag="MBZ71_002733"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Gln,seq:ctg)
     gene            complement(170..598)
                     /locus_tag="MBZ71_002734"
     CDS             complement(170..598)
                     /locus_tag="MBZ71_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430625.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(658..1416)
                     /locus_tag="MBZ71_002735"
     CDS             complement(658..1416)
                     /locus_tag="MBZ71_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBT4430626.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          417 a          277 c          275 g          494 t
ORIGIN      
        1 aaaaagattg ttcttttaca gggggttcat tttttcagca agcacatcaa attgggatgg
       61 cagggttcga acctacaatt gcttgattca gagtcaagtg ccttaccagt ttggctacat
      121 cccatttatt caacaaaata atcatagcac tttcctaaaa aaacgcaagt tatttttctt
      181 gatcggatag ttgttgcttt actcgtaaaa ggcggcgtac tagtttcttt tctaatccag
      241 caggaagggc aatcagttct gctactgaca agacggtgtc caataactga tattctttga
      301 caggttcatt cataatatga tagatgtttg ctaaacgttc aatatattca aagcgagtat
      361 actctgttcg agcaattaga aatttcaata aatcggtggc tttctcgtac tcttgatctg
      421 cgtaataaac aagagattga cgatacaatt tgatttccag ttcattgagg gtagcaggtc
      481 tgcgacgctc gttccactca ttaatagaga ctttaccttg aacatttccc ttgcagtcac
      541 ggacaaaaat aatcggaatt tcatttggat ccattttttt atgtgtgaat ttttccatta
      601 tctacgctcc cttcaaaggt ttggtatctt gagtataagg gatttttgtt tgaattacta
      661 gcttttctcc aaagttagca tctgtccacc cattgcagtg aaaatcgccg aaaaatcgtt
      721 gatcggtact gctcgatttt ttacaccata gggatcgttg acataaatat tttctttgtc
      781 atatccagtg actacacaag catgaacaag aggagtgact cttacttctc cattatttgt
      841 ggtccaagtc ataaaatcag tttcatctgg gacttcaaaa ttgatagtca cagaagtcca
      901 gacaggattt cctttagata gtgctttttt gatatcatcg agttgagcat cggtttttgg
      961 aaaagtgtga taatccgttt ggacgatttg ctgagcaagg gaggcaactg ggggaacata
     1021 gacgaccata gcccagtctc cgccactgat atcaccaaca aatccttcat taggatcgcc
     1081 atgagtccca tctgcattga aaacagggac gtaatccaat tgctcagcta actggttttt
     1141 agttgtttca taaccataaa aagataaaag catagaaagc gaagtgactt cacagccatt
     1201 ttctaaagaa ggagaatcaa actggctctc taatggaaca tctagtaaaa cttctgcttt
     1261 ctcttttgtc tttttcgttt tttgtgttga acttttttgt gcagtgttca ttgtatgttc
     1321 tggagaatgg atatgtaggt aataacttgt accgcttaat aaaagagcag ctgtgccccc
     1381 tagaaacaat agttttttga acgttttttt cttcatttga aatctccctt ttcagctgtt
     1441 tatctttcct attataaatg tat
//
LOCUS       DAGCWU010000260         1456 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.270, whole genome shotgun sequence.
ACCESSION   DAGCWU010000260 DAGCWU010000000
VERSION     DAGCWU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.270"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(261..1286)
                     /locus_tag="MBZ71_002736"
     CDS             complement(261..1286)
                     /locus_tag="MBZ71_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4430627.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT          433 a          235 c          284 g          504 t
ORIGIN      
        1 atggattgaa ataagggtaa atgtgtttaa aaaaacgaac tataaaaact tatacacaac
       61 gtggttaaaa atagtttttt cttccctgtg gaaaaaatag tttaacacaa acattatagt
      121 tatccacagc tatctaaaac agatggaaaa taagaaaaat tcgctaaaat accgggaggg
      181 ggtaacgagg gatgagtgat tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc
      241 tgttctttta taattctgtt ctacatttct tgttgttgga tcgaatctgt gagagtaatc
      301 tcggttgaaa gaatgattct tttttcttca gggtattggt ggtggattag ttggaataaa
      361 agttctgtgg ctttgattcc taactgattt gtcggaatgt ctatccgagt caactgtggc
      421 gaaaataggg aggacaataa gggatcactg aatcctgcga tcgaaagatc atcaggtatc
      481 tgctttccag attgttctgc cactttgtaa atactcaatg attttaatgc atcggtagaa
      541 aaaatcgcag ttggtctttc tcgtagcttc attaaatcat tcattgctgc catgctatct
      601 tctaagcgaa agccgccgtc tataatccat tcattacgga tatctaggtg gtgatcaaat
      661 aagcattgac ggtaaccatt gagacgatca ttggatacat aaacttctgt cggagcatgg
      721 agacagccga tatgacggtg tccattcttg atcagtgttt ccaccaaatc gtaactatcc
      781 tggaaattat ccgtgtccac tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt
      841 accacaatag gaatattgta ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag
      901 cttaaaataa taattcctcg gatcattttt tcttggatct ttttcgcgca tttttctagc
      961 tcttcttctg gtgtttttgc cgtttgtaga atcaagtcga agttattttg ttcagaaacg
     1021 gcagaaatgg attgaataac atctgaaaaa aaaggattgc cataggtcgt atgagaagta
     1081 cgatgagata atgctagaat cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg
     1141 ctcggttcat aattcaattc tttgattgct tgaagtacac ttgcttttgc tttatcagag
     1201 atatttggct ggttatttaa tactctggag acagttgatt ttgagacatt agcttttttc
     1261 gcaatgtcat aaatagtagg tttcaatttt atatgcacct cctttttggg agcgttttag
     1321 aataattcca tagtacaata gacattcttt tttgtctatg attttgaaaa tttaggaata
     1381 gcttgcatga tcatgagaga ccatattttt ttaagaagct attaattatt gatacataag
     1441 ctttcttatt tgtatt
//
LOCUS       DAGCWU010000261         1449 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.271, whole genome shotgun sequence.
ACCESSION   DAGCWU010000261 DAGCWU010000000
VERSION     DAGCWU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.271"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            83..817
                     /locus_tag="MBZ71_002737"
     CDS             83..817
                     /locus_tag="MBZ71_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HBT4430628.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          547 a          231 c          199 g          472 t
ORIGIN      
        1 tcaacacagt tcattatcaa ccaaacaaaa aataagtggt tataatggat tgttaatata
       61 aacctcatag gagtgggtta taatgaacaa aaaaaatata aaagatagtc aaaactttat
      121 tacttcaaag catcatataa atgaaatttt gagaaatgta catttaaata caaatgataa
      181 tattattgag attggttcag ggaaaggtca tttcacgttt gaattagcta aaaggtgtaa
      241 ttatgtaacc gccattgaaa tagatcctaa attatgtagg ataactaaaa acaaacttat
      301 tgaatatgag aacttccagg ttatcaataa agatatttta caatttaagt ttcctaaaaa
      361 taagtcatat aagatatatg gaaatatacc ctacaatata agtacagata taattcgaaa
      421 aattgttttt gaaagcacag ctacagaaag ttatttaata gtggaatatg gatttgctaa
      481 aaggttgcta aatacaaatc gttcactagc actattttta atgacagaag ttgatatatc
      541 catattaagt aaaatcccta gagaatactt tcatccaaaa cctagagtta atagctcgtt
      601 aattgtatta aaaagacacc cttcaaaaat atcactcaaa gatagaaaac aatatgaaaa
      661 ttttgttatg aaatgggtta acaaagaata cataaaacta ttttccaaaa accaatttta
      721 tcaagcctta aaatatgcaa gaattgacga tttaaacaat attagctttg aacaattctt
      781 gtctcttttc aatagctata aattatttaa tagataagtt aaggggtgca taaactgcat
      841 cccttaactt atcttatttt gcttaaacaa tcttggcccg taaactgcat aactcatatg
      901 taaatcgctc ctttttaggt ggcacaaatg tgaggcattt tcactatttc cggcaattac
      961 ttccatgtaa agtataaccc actatacttt gcaagcaaag tgtgggctcg ccgaggggtc
     1021 ttgcagacgg ctctttcggc tcttttgagc cgagtatcag cgcaccctta tggtgcaatt
     1081 agctgatacc tgtttgacga tttgctgccc taccttttcg gtaggtatgc aaacctcaaa
     1141 ccaaaataaa aacccccttc gacttcgtca gggggttttt atttatataa atctccgtaa
     1201 ataatcgcga tttaataaca tcttcaattt tatttttttg cgtattttag aaatgttttc
     1261 actaaaaaat atattataca aaaaatacag taatcgatta taattctttg ccagttaaat
     1321 atcatatcgt tattaaaata tcggaaaaaa atatcaacaa caataaatgc aaaagaagac
     1381 cataaaaatt gtttattact catacatact ctccatttct atcattttta aatttaccag
     1441 tcatctctc
//
LOCUS       DAGCWU010000262         1438 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.272, whole genome shotgun sequence.
ACCESSION   DAGCWU010000262 DAGCWU010000000
VERSION     DAGCWU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.272"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1192
                     /locus_tag="MBZ71_002738"
     CDS             <1..1192
                     /locus_tag="MBZ71_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4430629.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
BASE COUNT          574 a          205 c          305 g          354 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct gatggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      481 tcgttccgat gccgataaat acaatcctga aggacaaaaa gtgactacag acttaaataa
      541 agagccagac gcatctgagg gaattaagaa caaagaagat ctaccaaaag gaactactta
      601 tacttggaaa gagaaagtag atgttagtac agctggaaac aaaaaaggga ttgtagtagt
      661 aacatatcca gatggatcta aagaagaagt agaggttact atttctgtag aagataaaaa
      721 agcaccgaat aaacctcaag ttgatcctat tacagagggt gaccaaattg ttactggtaa
      781 aactgaacca aatgcagagg tgacagttac attaccaaac gggagtcaat accatgggac
      841 agctgataaa aatggtaatt ttacagttaa agttcctaaa ttagaggcag gaacaaaagt
      901 gatagtaact gcaactgatg aatctggtaa tactagtgaa cctactaatg tagttgtttc
      961 ttcaaacgaa aaagacagtg aaaaagccgt tagtaaagat aataagactg acaatcaagg
     1021 tagcaagcaa aacaaaaata gaggcaaaag cagtcctcaa aagcagtcct caaaagctta
     1081 tcctaagacg ggagagattg atagtaatat ctttactatt agtggtggtt taatcctgtt
     1141 aggaacttta gggttattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1201 tttcttaaat cttctctgaa aagaggaaga atctttttct tcctcttttt gttttttatt
     1261 ttgggagata ggttgttagt taaaaatgaa gatacttttc atagaaggga aaagaatgtg
     1321 attaagtgaa aaaaatagta attattggat caaaccattc ggggctagct gcagctcgat
     1381 ttctaaactt atgtgattca aattatgaag ttattctgat agataaaaaa tcagaaat
//
LOCUS       DAGCWU010000263         1427 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.273, whole genome shotgun sequence.
ACCESSION   DAGCWU010000263 DAGCWU010000000
VERSION     DAGCWU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.273"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            94..1389
                     /locus_tag="MBZ71_002739"
     CDS             94..1389
                     /locus_tag="MBZ71_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4430630.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          450 a          283 c          267 g          427 t
ORIGIN      
        1 tcaccaacat cttttttgta taaaaaaata tagaaaagac accctgaaat cctttagaat
       61 taatttggct accaattcat aggaggattc aagatgtctc atactcagct tatcaaagat
      121 accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac taaagaaaaa
      181 tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc agaagagtgt
      241 tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac aaccgttcgg
      301 ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa acaacgattt
      361 aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga aaaacactgt
      421 ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa tacttccatg
      481 acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt catgaagcga
      541 ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg ctttgatgaa
      601 ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga cggacaaagc
      661 catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg ctatttttct
      721 cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat gtatgcgcct
      781 tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga tcgtttccac
      841 atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac cacggtacga
      901 cttaaaggaa attcccactc tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa
      961 ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt
     1021 aaagattatt tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta
     1081 cgccaaggct atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca
     1141 ctatttgagg attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact
     1201 ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca
     1261 aacggaccat tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc
     1321 tttcgcaact tttacaattt caaactacga attatgattc gtcatggcaa aacattttta
     1381 accaaataaa cagaacagaa agaggttttc ttctttctgt tctgtcc
//
LOCUS       DAGCWU010000264         1424 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.274, whole genome shotgun sequence.
ACCESSION   DAGCWU010000264 DAGCWU010000000
VERSION     DAGCWU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.274"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..174)
                     /locus_tag="MBZ71_002740"
     CDS             complement(<1..174)
                     /locus_tag="MBZ71_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430631.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEET"
     gene            complement(276..629)
                     /gene="tnpB"
                     /locus_tag="MBZ71_002741"
     CDS             complement(276..629)
                     /gene="tnpB"
                     /locus_tag="MBZ71_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBT4430632.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(619..813)
                     /locus_tag="MBZ71_002742"
     CDS             complement(619..813)
                     /locus_tag="MBZ71_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430633.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(985..1245)
                     /locus_tag="MBZ71_002743"
     CDS             complement(985..1245)
                     /locus_tag="MBZ71_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430634.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT          416 a          281 c          244 g          483 t
ORIGIN      
        1 cgtttcctca aagagagtct ctttagagcg tccaaataat ttgttggtta aataattgac
       61 cttttcattc aatagttgga tttcagttgt aagattctga atggtttgtt gttgtgcttg
      121 attggtttta tgggcttctt gaagcaactg aagcaacgta tctgtagttt ccaaaatttt
      181 tcaccacctt gaatcgaact gattttgtta cagaatacca caagaaacgc acaagaaata
      241 ggggttcaac acattttttg tgttaacccc ccagcttaaa aaactccttt ttttcctggc
      301 aaaatagctc gaggttgatc gatacttaat ccttctaaca accatcgaac ctgttggttc
      361 gttaaggttc gaacttcgtc tttcgtccga ggccatttta acttgccgtt ttctatccgc
      421 ttataacaga gaagaaaacc atcaccgtcc caatagagaa gtttgaaacg gtcttgacgg
      481 ttcccgcaaa ataaaaaaac ggcatcttcg taaagctcga gttgatactc ttgttgaata
      541 agactggcaa gaccatcaat tccttttcgc aaatctgttt ttccgcacac aatataaata
      601 tgtttggttt gaagtaaatc ataaagcatt ggacaacagt tctttcaaaa tagcttgaag
      661 aatataattg ttaataccgt tatagatcaa aacttcagaa ccattctctc ttttgatgcg
      721 catcgctaac ttggcagccg cgggtttacc tttttctggt gcactctttt cgtttcctaa
      781 ttggacagga acaatctcat ttaatttata catagaaaac ctccagactt cttgatagct
      841 caagtatatc ttgaggtgca tttgttagta aggtactgtt ctatggggcg cttaccttgt
      901 gtcttcctaa aaaggtcttt tcctgtttct atgtaaaaca aaaagagctg caatcctcat
      961 ttaggactac aactcttctt ttatttatat gcccatcgat cgcgtaaaat gatcgggatt
     1021 tctaacatac atgctagcaa taactagctg atttcgttta ttgaagtgac ctgtgacggc
     1081 aatattgtat tttccatcat ccataattag caatttatcg ataatttcaa tatctgcaac
     1141 tacacaatta atcgattcat gaatcgtttg taaagtaaag cgaaccaata acggttgcgt
     1201 ttttattact cggattttat caagcaaacc aacatatttg ttcatcacaa tcacctcgct
     1261 aaaattatag caaaaatcca atcatttgga tatttcaatt atgctataat agtcttgctg
     1321 tctgctaccc cggcctaatc tggcaagggg aggaggttat aggtttgaaa attctttttg
     1381 attttgtttt tgcaatcatg gcagaagtag ccgcgcacta tatc
//
LOCUS       DAGCWU010000265         1423 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.275, whole genome shotgun sequence.
ACCESSION   DAGCWU010000265 DAGCWU010000000
VERSION     DAGCWU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.275"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            195..1061
                     /locus_tag="MBZ71_002744"
     CDS             195..1061
                     /locus_tag="MBZ71_002744"
                     /inference="COORDINATES: protein motif:HMM:NF025663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430635.1"
                     /translation="MNIHNIGKKILCRTFPIIMTKYFYRKILGRKLKLKKPETFNEKI
                     NWYKLYFCPQNDLVIQCTDKFRVREYVEDMYCGDSLNDLIGVWDGVDEINWNSLPQRF
                     VLKCNHGCGMNIICPDKTSLDIEETKNRLNKWMKMDWGKESIEPHYSKIKPKIICEKF
                     IENTLDKESSLPPDIKIHCFNGIPKVIAYYSGRGNDMHGTFYDVNWNKLDFATKEGSV
                     ISKPICLDKMLLYAEKLSNPFPYVRVDFYECNNIPVFGELTFTPASGRSPYMTREADE
                     YMGNLLNLKEME"
     gene            1063..>1423
                     /locus_tag="MBZ71_002745"
     CDS             1063..>1423
                     /locus_tag="MBZ71_002745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430636.1"
                     /translation="MRTKKAFYNTTTGIIYQLCSIVCGFIIPRLILGYFGSEYNGMIS
                     SITQFLGWISILTAGVGGVTRAALYKPLNKNDNYKISGIINATDNFMKKIALIFLGGL
                     IIFAIGYPFLLKNKFEWF"
BASE COUNT          546 a          156 c          247 g          474 t
ORIGIN      
        1 atatatcaac aatatattat taatagagag tttaaagcag caaaaaagtt taaaaatcaa
       61 tatcatatta atatattcac atcattaaaa gacttagtta tccatgtaat aaaaagatac
      121 tctattgcgt tgaaaaaaag aacattgtct tatgaagaaa tgttggtaga attagaaaga
      181 ggaatgaatt actaatgaat attcataaca taggaaaaaa aatactttgc agaacttttc
      241 ctattatcat gacaaagtat ttttatagaa aaattcttgg aagaaaactg aagttaaaaa
      301 aacctgaaac atttaatgaa aaaataaatt ggtacaagtt atacttttgt ccacaaaatg
      361 atttagtaat ccaatgcact gataaattta gagtaagaga gtatgtagaa gatatgtatt
      421 gcggtgattc tctaaatgat ttaattggag tatgggatgg agttgatgaa attaattgga
      481 actctctacc acaacgtttt gtgttaaaat gtaatcacgg atgcggaatg aacatcattt
      541 gtcctgataa aactagttta gatatagaag aaacaaagaa tagattaaat aagtggatga
      601 aaatggattg gggaaaggag tctatagaac ctcattattc aaaaattaag ccaaaaatta
      661 tttgtgagaa atttattgaa aatactcttg ataaagaatc ttctttaccg ccagatataa
      721 aaatacattg ttttaatggg atacctaaag ttattgcgta ctactcaggt agaggtaatg
      781 atatgcatgg aacattttat gatgttaatt ggaataaatt agattttgct actaaagaag
      841 gttcagtcat aagtaagcct atttgtttag ataaaatgtt attgtatgca gaaaaattat
      901 ctaatccttt cccctacgtt agagtagatt tttatgaatg taataatatt cccgtttttg
      961 gagagttaac ttttacacct gcaagtggaa gatctccgta tatgacgaga gaggcagatg
     1021 agtatatggg gaatttgcta aatttaaagg aaatggaata aaatgagaac gaagaaagct
     1081 ttttataata cgacaactgg aatcatatat cagttatgtt ctattgtatg tggttttatt
     1141 attccaagac tcattttagg atattttgga tccgaatata atggtatgat ttcatctata
     1201 acccaatttt taggatggat tagtatttta actgcaggag ttggaggagt cactagagct
     1261 gctctttaca aaccattgaa taaaaatgat aattataaaa taagcggaat aattaacgca
     1321 actgataatt ttatgaaaaa aattgctctc atatttttgg ggggacttat aatttttgct
     1381 attggttatc catttctctt aaaaaacaaa tttgaatggt ttt
//
LOCUS       DAGCWU010000266         1409 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.276, whole genome shotgun sequence.
ACCESSION   DAGCWU010000266 DAGCWU010000000
VERSION     DAGCWU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.276"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(807..1148)
                     /locus_tag="MBZ71_002746"
     CDS             complement(807..1148)
                     /locus_tag="MBZ71_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4430637.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1148..1378)
                     /locus_tag="MBZ71_002747"
     CDS             complement(1148..1378)
                     /locus_tag="MBZ71_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4430638.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          435 a          242 c          204 g          528 t
ORIGIN      
        1 agccataaca ccgcagcatt cacaacacct tataattttt gtcaaaaaaa gtgttgccat
       61 tccactgtat tgatagtgta cctaaaagct ctactgacat aagatcgtgt aatcggtaaa
      121 gtaattttat taattatacg ggatgtaatc aattagagaa taaactaatc ataaaaggta
      181 cttttacatt cataattgtc gctttgagat tctactttgg ttcactatct gacccaattg
      241 cacattttcg atcattacaa gtattggtgt cgttagaaca actaaataaa aactagcggt
      301 agatttttca gaatctatgc taatttctgt atggatgttc cagtgatggc aaatttctct
      361 tttcattgtt tttcttgtcg catgatatgc ctttttgtgt tattcagtta aactatagaa
      421 attctttcct agacagatct tgatcccaaa ttttacgttt ttgagcatcg atatagttta
      481 aaatgacatt actcatatct aaagagttta gattttttag gactaaaact gtcagttgtt
      541 ctttttcttc tttctgttaa agtattgtcg cgacttttcg tatacgttaa gatatgtcgt
      601 gaaaaaacaa gttttgtgac acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt
      661 cgtttaaaat gctatatatt ctttagttta agatacggaa attaatgcca tcgaccacac
      721 tagtagaatg aacgacattt acttggcctt tttttaattt cgttatgttt agcatataaa
      781 aatattatag agtttactta caatgcttaa aacatatact gtataatctg atcaatttta
      841 gccatgtctt gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga
      901 gattttaatt gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa
      961 cgtgtcggta aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta
     1021 cggttaaatt cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct
     1081 acggagggat caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct
     1141 tctgacacta taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat
     1201 aatactcggc ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg
     1261 ttccatcatc agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat
     1321 cagaaggtaa agttaccact acagaacttc cttgtaatct agattttgta acaagcatta
     1381 tcatcactcc cttaataaaa gtatactat
//
LOCUS       DAGCWU010000267         1408 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.277, whole genome shotgun sequence.
ACCESSION   DAGCWU010000267 DAGCWU010000000
VERSION     DAGCWU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.277"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(27..1046)
                     /locus_tag="MBZ71_002748"
     CDS             complement(27..1046)
                     /locus_tag="MBZ71_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBT4430639.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1057..1263)
                     /locus_tag="MBZ71_002749"
     CDS             complement(1057..1263)
                     /locus_tag="MBZ71_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBT4430640.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          388 a          300 c          233 g          487 t
ORIGIN      
        1 taatgctcct caacgaggag ctatttttat cgtttaggaa tatttaaata ccaacgtttg
       61 tcatggaaat cttgcgcacc gcctttagtg tttccctctg gatcattcgt tgcccgcatc
      121 atgacataga ctttcttatt agggaagtta cgcatgttaa aagatacatg atagccaaca
      181 tttccagaag tattataagc ttgatttaca tctgatctat aaattccatc agctcttact
      241 cgagctaatt ctttcccagt attgtaatcc ataatgaaga tatactcgta tttatagtta
      301 gcaatgtgcc atccagctac atgcaaattt gcattttcga tttctccaaa ctgatcaatg
      361 tgggcgtaat tcgttccatc tgtcagtgta ggattagctg caccagctcg tgttggatca
      421 atgacaggct tgttttcaga agttgttgga ttttcatcgg taaatccatg agctaaatca
      481 tatgctaatt tttctttact tacgcccatt tcagaaagat aaccgtaagg atctgtatga
      541 tcgccccaga tgttttgtgt tacccataaa tgcgatttga ttcccggttg gttataagga
      601 gtgtccaacg ttaatggaat accatatttc attgctgaat ctctagccaa ttcaacgtat
      661 gccttgtagt ttttctcaaa cgttgcttta tcatgtgtgt gttgtaactc aatctgcaca
      721 ggactgttgg cattagcata cgaaccagca ccgtactgta cataaccagg ttgaccgact
      781 tgataaacaa ttccgccgtc tcccacaata taagcagtat aagcgctagt ccatgaacgt
      841 tgcatatact gcgcttcatt gcgtcctgtt gctgtttcat tagccgtttc atgcagtaaa
      901 atatactgat tatttgctac ttgtgagcta ccttcatttg cgcccaaatt aaattcattg
      961 ttgatagtat aggcaaatcc gttaattggc aataaaaaaa gagccgttaa caggctcatc
     1021 gcagtaatag taattttctt tttcatatct atcctcctat ttatctgatg aaattttctt
     1081 atacgttcga tttgatacac ctaaaatact ccctagaaat gctccaaaac ctgtaataat
     1141 catcaccgtt atatctgtgt accgccagtt aacacctttt ccgacaagac cgacaaaagt
     1201 cgctaaagcg gggataacta ctatagcgat ccatttcaaa atatcaaagg ttctattgtt
     1261 catactatcc acctcccttc attggttggg ctactttctt tttcaatcgc ttgttttctt
     1321 ctatcaatat ttctctttct gaaagcaagg catcaatttg cccgtccagt tgttcaattt
     1381 tatctttctt aattgtcaaa ttaagtta
//
LOCUS       DAGCWU010000268         1402 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.278, whole genome shotgun sequence.
ACCESSION   DAGCWU010000268 DAGCWU010000000
VERSION     DAGCWU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.278"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1352
                     /locus_tag="MBZ71_002750"
     CDS             <1..1352
                     /locus_tag="MBZ71_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBT4430641.1"
                     /translation="DEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLL
                     PLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKK
                     AIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIK
                     AVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYY
                     HHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKK
                     NTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAK
                     SKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGART
                     SGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          465 a          254 c          299 g          384 t
ORIGIN      
        1 gtgatgaaga aatttctgta agtgttccag aagcagctgc caccattatt ccagtaaaag
       61 gacatcaacg agttgtagga acgaaagcag acaaaatcaa acacttacct ataacagaga
      121 aagaacatct ccttcctttg gaagaacaat tttgtgaaca ttgcggttca cagatgaaag
      181 atatcggacg gacaaaagta cgagaagaaa ttcgtttcca tcaggctatg ttggattgtc
      241 tcacccatta tcaacatacc tattgttgta aaagctgcga aaaagaagga ctttcttctt
      301 ttaaaaaagc gatcgttcct aaacctttga tttctaatag cttaggttcc aatagtttag
      361 tggcggaaac gatccggatg aaatttggtc agaaagttcc tgcctatcgg caagagaact
      421 attggaaaca aacacatggg ttggatattt cgagagataa tatcaccaat tggcatatta
      481 aagcagtgca aaacgcgtta gaccctttag gagaacgatt aagagtctat cttaatcaag
      541 aagagattct acacggggat gaaacaagtt atcgtgtgat tgaaagtgcc aaaacagata
      601 cctattactg gcagttttgt acaggaaaag acagtcagca tcccatcgtt tattatcacc
      661 acgacgaaag tcgtgctgga gatgttccga aaacttttct caaggagttt acagggtatt
      721 tacactgtga cggctatagc gggtataatg ccgtggaaag tgtacgactt gtctattgtt
      781 tcgcgcatgt tcggcggaaa tttttcgaag caattccaaa aggaaagaaa aacacggata
      841 ttcccgcagc tcaagcagtc aaacaattgg ataaatggtt tgtcctagaa aagaaatgga
      901 aagacttttc tcctgagaag cgattaagct gtcggcaaca agaactgcgt ccattattta
      961 ttgcttttta tgagtggatg gcaacgatag atcctgttgc aaaatccaag ttggatgcag
     1021 ccgttcaata tgcgtgtaaa ctacgaagtg gttttgaacc tatttttgaa gatggtcgtt
     1081 tagaactcac aaataatcgt gcggagcgaa atatcaaaga gctggtgata ggacggaaaa
     1141 attggttgca ttccacaagt cttgaaggag cacgaacttc aggaatcatt ctgagtgtat
     1201 ataaaacagc agaacttaac ggattgaatc ctgtaaagta tctagagttt ctatttgata
     1261 agataccaaa tcttcccgta ctttcagcag aaactttaga tcaactgttg ccttggaaca
     1321 aggatgtaca acaacatttt tctcgtaact aaaaacgcca aacattcccg attctattct
     1381 ataggtcggg ttatttgacg ta
//
LOCUS       DAGCWU010000269         1399 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.279, whole genome shotgun sequence.
ACCESSION   DAGCWU010000269 DAGCWU010000000
VERSION     DAGCWU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.279"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            353..895
                     /locus_tag="MBZ71_002751"
     CDS             353..895
                     /locus_tag="MBZ71_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430642.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1051..>1399
                     /locus_tag="MBZ71_002752"
     CDS             1051..>1399
                     /locus_tag="MBZ71_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBT4430643.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          549 a          168 c          267 g          415 t
ORIGIN      
        1 aggcttacca gcgagataga gatcgacgat ttgttgtttg aattcttttg aataggttct
       61 tcgttgacgt cttggcatga aaaatcctcc agtgtgtttt ttattattct acacacctta
      121 ttttttctgt ctagtctagt gtagccgatt caaattgatg aagcagatcc caaagggctg
      181 cttcatcaat tttttcgtaa gtgtccgtac acttactgtg cttgctcata tattaatcga
      241 tcatatacta atcgtgtaag aacaacttta agcataatac ttatattgtt ataccatagt
      301 ttggaaaaat aaaaaattat atttcatcaa cgtttcataa aaggtgtgta gaatgttatt
      361 gaaagtagct ataaaatgct attgtgtaaa aaataaagga ggaataattt atgaaaaaat
      421 agtaccaaga ggagcatgcc tattacttgc attatgtttg agcttaagcg cagttgggtg
      481 tcaaaaaagt aatgaaaaca ataaaataaa agaaggacaa tccataagtt ctgaagagga
      541 aggcatgtca attaatgcaa atggcaaaaa tgaaactttt aagccatcag attatacatt
      601 ggaggcaaag aaagaatatg tttatgaata tttagggctt aaatttaaac tctcagacaa
      661 atttagaaat tatatagctg ataaaaagat agcgatgtta gatgatcaaa gtcctataga
      721 taaagaacta aagtatgcta ttttgacatt tgagaaaatg acagaagaac aaaagaatgc
      781 agttatagaa aagatgggcg atggatataa aaattggcaa aatgagcttg aaagaattgg
      841 gactattggt atatttgaaa aaaatacatc agaagaaaaa aatctaaaat tataaaatgt
      901 gatactcata ctaaaatagg agtttcaagt gatggaaaat acgattgcta tttaagcaca
      961 aatagcggag ctgaaagtaa tcttcttgat gaattaaaaa gaactgagat tcaaattata
     1021 gataaaaaag aacgtcctaa aaatggtttt gtgctatcag aaaaaactga tttagaaaat
     1081 acagaagcat ttaacaaaga atcggttaaa gatttacgta aactaccaac aaaggacata
     1141 aatggcaagg attttacaag taaagatttt gagaagtatg atcttactat ggtaaatgta
     1201 tttgcaactt ggtgtacagc ttgtgtaaag gaaattcctg atttggttga ggttcagaat
     1261 gaaatgaaaa gcaagggtgt aaatatcgtt ggagttgtta cagatcctgt tgatgataac
     1321 ggtgaaaaca aggaagcaat tgaaaagtca aagttaatac atgaaaagac aaaggcttca
     1381 tatcctttct taatgccag
//
LOCUS       DAGCWU010000270         1397 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.280, whole genome shotgun sequence.
ACCESSION   DAGCWU010000270 DAGCWU010000000
VERSION     DAGCWU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.280"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(73..480)
                     /locus_tag="MBZ71_002753"
     CDS             complement(73..480)
                     /locus_tag="MBZ71_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430644.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(499..1317)
                     /locus_tag="MBZ71_002754"
     CDS             complement(499..1317)
                     /locus_tag="MBZ71_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4430645.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          409 a          233 c          193 g          562 t
ORIGIN      
        1 tgtataactt atactttaat attcatttat ctagtataac ttatacttta attgaatacc
       61 gcatgaaaaa aattattttg gtaatttttc aaactcttct tgtatttctt ctcttatgtg
      121 ttcttctttt gctactccta gtctatgctg gatatatgag tctgctaatg tattaacaat
      181 tttttcgaaa gaaattctcc ctttttcact ttgtaactcc gtcatatagt cttgtaaaat
      241 tgagagtttg ctatttaatg ccggagagat ctttttagga actgatacct tgtttctttt
      301 atcaggaatt gaaactggtc ggcctctttt tttcaatgta atatttcttg tgttactcaa
      361 tgcactattg ctaaagttat attcaccgct tattttcaca tcttggcgtt tgttattggg
      421 taataatggc tcaaattcac cattctccat cattttagga atatcaaaat ctttaggcat
      481 tacttatgca actccttatt atttatttaa aaactcttgt ctagcatcta gttctgccaa
      541 aatatttata aatacttggt gtgctttata atcccattga tcaaaatttc catttttgta
      601 ttttcttaaa tgtataccaa ttttagaata ccttctcaat cttttttgat aatgaatgag
      661 atttttggat accacggttc cgtatttttc aattgcttct tctaggattt cttcatcaaa
      721 atcatcattt ggttctacca tacaaggtac aatatctata atttgcaaat caagatcgta
      781 tctatcgacc atgaattttt gataatttac atatttgttg actccctcta aggattcttc
      841 agctgtttga aatgaaatta tcgaatagtc tgaagctgcc atagcattat cggaaaattc
      901 gctaatggtt ggtggtacat caataaatat ttcttgataa ttgttcttta ttggaagaag
      961 taattttttc aagacagtaa cttggtctaa ttcattttca tgtttattag caaaagcgga
     1021 gaatgatcga aaactagtgt cacatgccag aaggtcaaga ttttcattta ccttaataat
     1081 actacttgct aattctccat ttgcgacacc atttgttata gtggttttga tatcggttat
     1141 accgaaagtt cttgctatga cttgtgttaa gttcgcttga gggtctaaat caataataag
     1201 cgtttttcta tttcttatca ttgcattgtc ataagcaagc atgctgacta tcttagtttt
     1261 acctactcct cctttaaaat tgccaactgt aaatgtagtc ccttgaaaag tatccatgta
     1321 aatcctccca atctttaatt gataattaaa gtataactta tatatcggaa atggtcaaca
     1381 ttaaaagtat aacttaa
//
LOCUS       DAGCWU010000271         1386 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.281, whole genome shotgun sequence.
ACCESSION   DAGCWU010000271 DAGCWU010000000
VERSION     DAGCWU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.281"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..557
                     /locus_tag="MBZ71_002755"
     CDS             <1..557
                     /locus_tag="MBZ71_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4430646.1"
                     /translation="FSYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNLYSEEKNCV
                     LDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHEGMANTNLE
                     SAASGRPIITSDIPGCKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYEQRINMGKM
                     GRNYMIKKFNKKKVVDETIKSLFS"
     gene            603..>1386
                     /locus_tag="MBZ71_002756"
     CDS             603..>1386
                     /locus_tag="MBZ71_002756"
                     /inference="COORDINATES: protein motif:HMM:NF025025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4430647.1"
                     /translation="MSNVSNPLVSVIMGAYNSEKTIGRAIDSIIKQTYLHWEMIICDD
                     GSTDGTVKIIRKYVEKDKRIKLVQNKKNSGLAYSLNHCLKYSSGKYVARMDADDISLP
                     ERFAYQVGFFEKNPEYDVVGCNRIVFDENGDSGIRKSKEIPTINDMYKGSPFAHPTIM
                     MKRSVYTILGGYRVNKDTTRSEDLEMWFRFFNEGFRGYNLQRVLFKYQETSEDFHKRS
                     LRAAYGITKVLLRGYKLIGVSKYKYIYAFKPIISAIIPKYIMY"
BASE COUNT          545 a          145 c          272 g          424 t
ORIGIN      
        1 actttagtta tttagaatat cctaaagatg agcaagttca ttttttattt attggaagaa
       61 ttatgaaaga gaaaggtgta gatgaacttt ttgaagcaat gaaaaactta tatagtgaag
      121 agaaaaattg tgtacttgat gtccttgggt attttgaaga agattacggc ataaaaattg
      181 aaaaatacga aaaagaggga tggttaaaat atcatggata tcaatctgat attcgtccct
      241 ttgtagaaag agcacattgc tttgttttgc ctagttggca cgaaggaatg gcaaatacta
      301 accttgaatc agcagcatca gggagaccaa ttattacaag tgatatccca gggtgtaaag
      361 aagcagttat tgaatcgaag tctggttatt tatgtgaaag aaaaaatagt tgtagtctct
      421 ataattcgat gaataaattt ctaaaattat catatgagca gcgcattaac atgggaaaaa
      481 tggggagaaa ttatatgatt aaaaaattta ataagaagaa agttgtcgat gaaacaatta
      541 aatcattatt tagttaaaaa gatatgtttt gcaaagtatg tgatagaaaa gagggattct
      601 gtttgagtaa cgtaagtaat ccattagtaa gtgtaatcat gggagcctac aattcagaga
      661 aaacaattgg aagagcaatt gattcaatta taaaacaaac atatttacat tgggaaatga
      721 taatttgcga tgatggatca actgatggaa ctgtaaaaat aatacggaaa tatgtagaaa
      781 aggataaacg tattaagctt gttcaaaata aaaaaaatag tggattagca tattcattaa
      841 atcattgttt gaaatattca tcgggaaaat atgttgcaag gatggatgca gatgatatta
      901 gtttaccaga aagatttgca taccaagttg gattctttga aaaaaatcca gaatatgacg
      961 ttgtaggatg taatcgaata gtattcgatg aaaatggtga ttctggaata cgaaaatcaa
     1021 aggaaattcc aactattaat gacatgtata aaggttctcc atttgctcat ccgacaataa
     1081 tgatgaaacg aagtgtttat actattctag gtggttacag ggttaacaaa gatacaacaa
     1141 gatcagaaga tttagaaatg tggtttcgat tcttcaatga aggatttaga ggttataatt
     1201 tacaacgagt cttatttaaa tatcaagaaa caagcgaaga ttttcataaa agaagtttaa
     1261 gagcagctta cggaataaca aaagtattgt tgagaggtta taagttaata ggagttagta
     1321 aatataaata tatttacgca tttaagccga ttatttccgc aataattcct aagtatatta
     1381 tgtata
//
LOCUS       DAGCWU010000272         1377 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.282, whole genome shotgun sequence.
ACCESSION   DAGCWU010000272 DAGCWU010000000
VERSION     DAGCWU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.282"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..785
                     /locus_tag="MBZ71_002757"
     CDS             <1..785
                     /locus_tag="MBZ71_002757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430648.1"
                     /translation="KDKIFKFLLLFTMPILLKTVADGYLSRNNIAVYLAFIGIYLVKE
                     KYIPKTVAKFSLLIIIPIGLFLFYIMSQIRLGTQSDISFWQGTWNLIDSESSYPQYYD
                     YANQGNSNVNIINFIIYIFIVCIPSSFYDLIHFSIPNLAYTFTETILGLSYGETNNYY
                     ILLPSVLGEAIMLCGKYFAFTYAFIYGIVSTWFLRLLKRNKSLEYLLIYYLLDFFRQF
                     RGGSQYVITAWETQLVPLIIIVFFYKLVIKNGVAKERNEYNV"
     gene            769..>1377
                     /locus_tag="MBZ71_002758"
     CDS             769..>1377
                     /locus_tag="MBZ71_002758"
                     /inference="COORDINATES: protein motif:HMM:NF021616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4430649.1"
                     /translation="MNTMCEVSIIIPSYKGSNEILIAVWGALSQTVTSKEIIVVDDNG
                     IGTEEQIKTKEKLQKFIKENKIKYFAHEKNLNGSAARNTGIKASNGKYIAFLDDDDYI
                     IPSKLEEQINVLEKTPKEVGMSVCEGYYVHKDGKGYLKKIKGNYVALDKYLLDKHYFN
                     TSSIVIKRECLIDMKGFDDSFIRHQDWEFCTRMLSRYKSIVVH"
BASE COUNT          520 a          148 c          237 g          472 t
ORIGIN      
        1 taaaagataa aatatttaaa tttcttctac tatttactat gccgattctt ttaaaaacag
       61 tggctgatgg atatttatca agaaataata ttgcggtata cttagcgttt attggtatat
      121 acttagttaa ggaaaaatat attccaaaaa cagtggcaaa attttcttta ttaattatta
      181 taccaatagg actattttta ttctatataa tgtcacaaat aagacttgga actcaaagtg
      241 atataagctt ttggcaagga acatggaatt taatagatag tgaaagttca tatcctcaat
      301 actacgatta tgcaaaccaa ggaaatagta atgtaaatat aattaatttt attatatata
      361 tatttattgt atgcatacca tcaagcttct atgatttgat tcatttttca attccaaatt
      421 tagcgtatac tttcacagaa actatattgg gtttgtctta tggggagaca aataattatt
      481 atattttatt accatctgta ttaggagaag ctataatgct ctgtggtaag tacttcgcat
      541 ttacctacgc gtttatctat ggtattgttt caacttggtt tttgagattg cttaaaagaa
      601 ataagtcttt agaatattta ctaatttatt atttattaga tttctttagg caatttcgtg
      661 gtgggagcca gtatgttata actgcatggg aaacccaatt ggttccttta ataataatag
      721 tattctttta taaattagtg ataaaaaatg gagtagcgaa agagaggaat gaatacaatg
      781 tgtgaagttt ctataataat tccaagctac aagggaagca acgaaatact aattgctgta
      841 tggggagcgt tgtcacaaac agtgacatcg aaggagataa ttgtcgtaga tgataatgga
      901 attggaacag aagaacagat aaaaacaaaa gaaaaattac aaaaatttat aaaagaaaat
      961 aaaataaagt attttgcgca tgaaaaaaat ttaaatggtt cagctgcaag gaatactggg
     1021 ataaaagcta gtaatggaaa gtatatagct tttttagatg acgatgatta tattattcca
     1081 tctaaattag aagagcaaat taatgtatta gaaaaaacac caaaagaagt tggtatgtca
     1141 gtatgtgaag gatattatgt tcataaagat ggcaaaggtt atttaaaaaa aataaaggga
     1201 aattatgtcg cattagataa atacttatta gataaacatt attttaatac gagttctatt
     1261 gttataaaaa gagagtgttt gattgacatg aaaggctttg atgattcttt tatacggcat
     1321 caagattggg aattttgtac gcgaatgcta tcaagatata aatctattgt ggttcat
//
LOCUS       DAGCWU010000273         1375 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.283, whole genome shotgun sequence.
ACCESSION   DAGCWU010000273 DAGCWU010000000
VERSION     DAGCWU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.283"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            191..445
                     /locus_tag="MBZ71_002759"
     CDS             191..445
                     /locus_tag="MBZ71_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4430650.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="MBZ71_002760"
     CDS             446..763
                     /locus_tag="MBZ71_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBT4430651.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          420 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atatt
//
LOCUS       DAGCWU010000274         1367 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.284, whole genome shotgun sequence.
ACCESSION   DAGCWU010000274 DAGCWU010000000
VERSION     DAGCWU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.284"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            26..1365
                     /locus_tag="MBZ71_002761"
     CDS             join(26..467,467..1365)
                     /locus_tag="MBZ71_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBT4430652.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          573 a          176 c          237 g          381 t
ORIGIN      
        1 aatctaatta aaaggggtgc tttttttgcg gaaatataca tttgttttta agaaaaaagt
       61 agtttcagac tatttaaata acgaaggcgg ctacaaatat cttgcacata aatatcaaat
      121 aaatcgtaca ctggttagac attgggtaag gatttataac tatcatggtt gggaaggctt
      181 ggttggaggt ggcaaaagct acactacaaa atttaaactt gatgttatag aatatatgga
      241 aacaaatggt ctttctatcc aagaaactgc taaaaaattt aatatcggtt caaatagaac
      301 tctaagtaaa tggatagagc aatatgaaga aggcggtgct tcttcacttg agagccaaaa
      361 aaggggcaga aaaattagta tgaattccaa gctaaacatt cctaaaaaac ttaaagatga
      421 gtctcttgaa gaagaagtta ttcgtttaag agcagagaac gcatatttaa aaaagttaga
      481 aaccttgatt caagaacaag atttgagcaa gaagaaatca aggttaaaac aatccttcag
      541 ctcaaaaaag aatttaaatt gaatctatta ctttcaattg cacaattagc gaagtcaact
      601 tattattact gggtaaaaaa attagataag ccagataaat atagcaagat aaagcaagaa
      661 attacagcga ttgtaaaaga atcaagaaac tcttatggtt atcgtagagt cactttagcg
      721 ttaaagatga aaggatatac aatcaatcat aaaacagtta gaaaattaat gtcccaaatg
      781 ggacttacct gtcaaatccg aataaagcga tataaatctt ataaaggaac agtaggaaaa
      841 attgccaaga atgtgttaaa acgaaatttt tcagtagata cacccaataa aaaatgggtg
      901 acagacgtca ctgaattcaa gataaaagga agaaaaatct atctatctcc tatacttgat
      961 ttatttaacg gagaaataat tagttatagt atatcaacta gtccaacata taaactgatt
     1021 gaagaaatgc ttcaacaagc aattaaaaaa aagggaactg agggttcact gattctacat
     1081 tcagatcaag gatggcagta tcagatgcca caatatcaaa aaaaattaaa agagaataac
     1141 attattcaaa gcatgtctag aaaaggaaat tgcttagata attctgtaat agaaaatttt
     1201 tttggtgtgc ttaaatcaga gtttttttat cgagaaaaat tccgatcaat tgagatattt
     1261 caaagtaaat taaatgagta tattaggtgg tataataaca aaaggataaa actaaagtta
     1321 aatggactat ctccagtaga atatcggaaa cagtccatta aatagtt
//
LOCUS       DAGCWU010000275         1364 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.285, whole genome shotgun sequence.
ACCESSION   DAGCWU010000275 DAGCWU010000000
VERSION     DAGCWU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.285"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(140..1288)
                     /locus_tag="MBZ71_002762"
     CDS             complement(140..1288)
                     /locus_tag="MBZ71_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4430653.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAGCWU010000276         1364 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.286, whole genome shotgun sequence.
ACCESSION   DAGCWU010000276 DAGCWU010000000
VERSION     DAGCWU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.286"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            80..>1364
                     /locus_tag="MBZ71_002763"
     CDS             80..>1364
                     /locus_tag="MBZ71_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBT4430654.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          465 a          263 c          257 g          379 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccctttttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat
     1261 agaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt
     1321 tgagaacatg agaattagaa tctttttgat caatcaatta atca
//
LOCUS       DAGCWU010000277         1323 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.287, whole genome shotgun sequence.
ACCESSION   DAGCWU010000277 DAGCWU010000000
VERSION     DAGCWU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.287"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            208..762
                     /locus_tag="MBZ71_002764"
     CDS             208..762
                     /locus_tag="MBZ71_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4430655.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1016..>1323
                     /locus_tag="MBZ71_002765"
     CDS             1016..>1323
                     /locus_tag="MBZ71_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430656.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT          537 a          136 c          233 g          417 t
ORIGIN      
        1 attaagagct tagaatcttc atataagtaa ggttaatttt tttgtacaaa atagtcggaa
       61 tattctgagt tactgaaatg atataaaatc ataaactaaa ccccttgatt ttcttcactt
      121 ttaacagagt atatatataa gttcatgatt atggtaaaat atatatataa tcatgaactc
      181 agaatatttt taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat
      241 gaagagatac aagattttca gttttggcta agaagaacaa gtaatccaga aagagatagt
      301 tttttatttt tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa
      361 gttggagaca tacgaggtaa aagtaagcct attattgttg aacataaaac aggtaagcga
      421 aaaccgattt ttatagataa tttgagagaa gagattttat tatatactga aggtaaagaa
      481 gaaaatgatt ggttatttcc aagtcggcaa caaggtcgac atataacaag ggatcgtgtc
      541 tatcagatat atgcagatat agcagaaaaa ttaggaagag atgatatagg aacacataca
      601 ttaagaaaaa cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg
      661 tttattttta atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat
      721 gaaatagggg catcgttacg tggttttaaa ttgggaggct aattgagata ttaggatata
      781 ttttttgtgt ataatgaaaa cttagaaggt gagattaatg aagataactg aattttcgga
      841 agtatttaaa acgtttagta acatttacaa tagaatacca gagatagaac aaaatgttgt
      901 agttgataat gattattata tagagaatga ttttaataaa ccaagaaaat atgacaacct
      961 atacaatttt atacacaata gaacaacaat aaataacaac agtaacactc gagttttgat
     1021 taattcagca tattttcata ttaaatattt aaagaaatat gagtatgaag aagttgtatt
     1081 agattatggg ctaaatagac agtcattttt aatttatgca ataaataacg gaaatttgcc
     1141 aagcaataaa aagaacataa ttattgaaac atatgcctat ctggaggggg gatctaaaaa
     1201 actaattaag tcatgtaata tagagtatca attatcagta ggagaaatta aatgtgtttt
     1261 cgaagaagat ttagaacttg acttaaaaaa aatttttgcc agcattcatg attatatagg
     1321 ttt
//
LOCUS       DAGCWU010000278         1307 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.288, whole genome shotgun sequence.
ACCESSION   DAGCWU010000278 DAGCWU010000000
VERSION     DAGCWU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.288"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            381..713
                     /locus_tag="MBZ71_002766"
     CDS             381..713
                     /locus_tag="MBZ71_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4430657.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            623..826
                     /locus_tag="MBZ71_002767"
     CDS             623..826
                     /locus_tag="MBZ71_002767"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4430658.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            1010..1165
                     /locus_tag="MBZ71_002768"
     CDS             1010..1165
                     /locus_tag="MBZ71_002768"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBT4430659.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          468 a          231 c          231 g          377 t
ORIGIN      
        1 gaatgatttt aattattaac gttttttaat tcgtttttag cttaaaaata acttttgtca
       61 cagtctcact cctaaaatta ctttatttac gtacgaagtg ggcaggaggt cgaatccaga
      121 actgggccat ggtacttcaa cgaggacttc tcctctagaa ttgaaaagca tgcgatttac
      181 cttccgtaac gacggtagat ttattcaact atgtgtccaa tcaaaatttg tctgatttca
      241 aaaatagtca agagaatttt ggttgttaaa aactcttatc tatcaacttc cacagtttag
      301 ttgacaaacc ctgatactaa gttgggctat gatacacttt tcttgactat cagttacgtt
      361 accggaagga atgaactcta ttgaaaaaaa catatgatcg taaatttaaa ctaaagattt
      421 cacaggatat ccttgagaaa aaagtaacta ccaagaaaat tgctgaagaa tacaatatct
      481 ctcgtccaac aatttcccga tgggtttcag aatatcgtcg atacgggaaa aatgcttttg
      541 ctggacaagg aaaaagatta cctgataaag cagatttcta cattcttgaa caggaaaata
      601 aacgactaaa agaggaagac gagtgttcta cgaacacaaa gatcgatacg gatctccagg
      661 gatcactcaa aaactttacg atgaaggaat cgaaatcaat aaacgtgttg tagctgttct
      721 tatgcgtgaa atgaatttat gcacaagagg atttcatcga cgcaaatctt ctcatggtag
      781 agaaaaaaca atcaaagaaa tggtaaaaaa aatgttgaat cgataatttg aacagaacca
      841 aattgatgct gtttgggtga cggatattac ttacattcca tgtaatgacg gtcgcctcta
      901 tttgtccacc tatattgact tagcaactcg tattctacgt tactatatgg tggattcaca
      961 catgaaaaaa gagattgtcg tccgtccatt acaaatttat taaggagaat tgcctacggt
     1021 aattcattcc gatagaggta gccagcatcg gtcttttatt tatcaagagc tgttgaccga
     1081 ccaccacatc acacattcaa tgagccaaac tgggactcct gttgacaatg cagtaattga
     1141 agcgtttcat cgatcaatca aatgagaatt aatcgactcc aacaaatata aaagtaaaat
     1201 agagatgaag gtgttaatcc aagattattt agaagactac tacccaaaca aaagaatcca
     1261 tacaaaattt atgatgactc cttgacaatt tgaagcgaag aaaataa
//
LOCUS       DAGCWU010000279         1288 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.289, whole genome shotgun sequence.
ACCESSION   DAGCWU010000279 DAGCWU010000000
VERSION     DAGCWU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.289"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            6..611
                     /locus_tag="MBZ71_002769"
     CDS             6..611
                     /locus_tag="MBZ71_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4430660.1"
                     /translation="MQIISLSDLLEVAPDLESVNSILKTFKSIPHPITGQVNDVEYFL
                     HQKAIEFEKAALATTHLLFSSYKDKSILVGYFSLANKSLIMSKKNYNNLSKPQQRRLC
                     QNGSKTETGGYIVNSYLIGQVGKNYSDEAQKIDAINGTQILTIAYDTVLQAKKIINAR
                     YVWIECENIPRLIDFYRDFGFEVIENYETNSGLVVMVMKLK"
     gene            963..1036
                     /locus_tag="MBZ71_002770"
     tRNA            963..1036
                     /locus_tag="MBZ71_002770"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:997..999,aa:Ile,seq:cat)
BASE COUNT          443 a          181 c          231 g          433 t
ORIGIN      
        1 tgggaataca gattatcagt ttaagtgatt tattagaggt tgctccggat ttagaaagtg
       61 taaatagcat actaaagaca tttaaatcta ttccacatcc tataactggt caagttaatg
      121 atgtggaata ttttttgcat caaaaagcaa tagaatttga gaaagccgct ttagcaacta
      181 cgcatctttt atttagctca tataaagata aatctatatt agttggttat ttttctttag
      241 ctaacaaatc cttaattatg agcaaaaaaa attataacaa tctcagtaag ccccaacaaa
      301 gaagattgtg tcagaatggt tcaaaaactg aaactggtgg ttacatagtt aatagttatt
      361 tgataggaca agttggcaaa aactattctg atgaagctca aaagatagat gctataaatg
      421 gaacccagat attgacgata gcttatgata cggttttgca ggcaaaaaaa attattaatg
      481 ctagatatgt atggatcgaa tgtgaaaata ttccgaggtt aattgatttc tatagagact
      541 ttggttttga ggttattgaa aattatgaaa caaacagtgg attagttgta atggttatga
      601 agttaaaata gtaaaactaa ttattgcatg tatctattac aaggttaaac aagcgtatag
      661 tgaacttaaa aagatttaaa tttgtaaaac aaaaacctca accagtcatc cttggttgag
      721 gcttttttgc tctgtaatta actcgtatag atcctgcaag atagtttgtt tctgaataaa
      781 tgattgatat gaagtattta gataactata ttgacaacta taaaaatcat tcgataaaat
      841 agtgatgtta tcgcatatct tcactatcac ccataatagt cacactccaa gctatgcgat
      901 aacaggtttg ttgccacaca ttctactggt tgattgttta tggctttatg tggcaacaac
      961 cagtaccctt agctcagttg gtcagagcag acggctcata accgtccggt cgtaggttcg
     1021 agtcctacag ggtacattaa cgtagccatt tgaatcgttc tgtgttagaa ttttttgaag
     1081 agtattatac aagctaaagc ttttcttcat tgccactcaa atgagtggct tttttatgta
     1141 tccttttatg gattaatgaa aggatgtttc acatagttat atttctgtat atttgaaaag
     1201 ttttactttg atttttaaat agaaagacat ttgggttaaa ttgtgagatg ataataaaga
     1261 agagtttaaa gcgcacccca agccactt
//
LOCUS       DAGCWU010000280         1276 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.290, whole genome shotgun sequence.
ACCESSION   DAGCWU010000280 DAGCWU010000000
VERSION     DAGCWU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.290"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            527..913
                     /locus_tag="MBZ71_002771"
     CDS             527..913
                     /locus_tag="MBZ71_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4430661.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1011..1202
                     /locus_tag="MBZ71_002772"
     CDS             1011..1202
                     /locus_tag="MBZ71_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4430662.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          483 a          189 c          188 g          416 t
ORIGIN      
        1 atttttcatc atgatgaata ctatttacat atatctagtt caaagacaat aattattatg
       61 atccgttatt atctaagcac tatacagata ataggaattt ttcgtttcta aatactccaa
      121 tttgggaatc caataaaaat tttcgttcaa atacgtgtct attcgaactc ttttctagtt
      181 gttaattatg aagggggaag aaataaaaaa tacaatctat acacgaataa gaatattatc
      241 caaaaggagg ttttagaaaa gatttattat acgtaaaaag ctcaagacat tttcttagcc
      301 ataaggacat gtttgatatt taagaagata tatgaatttt cttttctaag tataaagata
      361 actatccatt atcaactaaa aaaacatttt tgaaaataaa catcagcagt cctatcaatg
      421 catgtgagct tatagaagtt attcgcagcg cagcataatt gcattagctt cttttcgtgt
      481 tttttgcctc ttttcccttt aaatcttaat ggaggaggtg tgcaatatgt cagataacga
      541 cgaacgcgta aatgcccagt tgattcgatt ctttgaaaag gtcattttga acaccgctaa
      601 tacttattat aaaaaacaag aagaaattca aaaacatgaa caacttgatc aactcccacc
      661 atatttgttt actaaagata ctataaaatt taaacaaccg attaatgttt taaatgtgac
      721 tctccttgtt gaagatataa aattagcaaa aatccttcct ttgttgaaag aacctgaaca
      781 aatttttctt atcgaaaaat ttatctttaa taaaactgat aaagaaattg gaaaggttct
      841 gggtattaca agacagggtg cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa
      901 aatgctggtt taatatctgc ataataacta attttgtatg cttctttgct cgtcctcctc
      961 cttcccaaaa aaataaatag attataagtt catgggaaga aggtaaattt atgaatactt
     1021 taaaagaaga tttcatttta gcaaagaatg gaaatgaaga agcagttgtg gccattctca
     1081 aaagatttag cgctttggtc cataagcaat cctggagaac tggaaaatat gatcaagatt
     1141 gttatcaaga atgtatgctt gcaatatatc tagctatctc gaaattcgaa attaaagaat
     1201 aactcaacta agggagtctg tgacataagt aaaaagatag tttcaaattt cgaacaataa
     1261 atttgaagcc atcttc
//
LOCUS       DAGCWU010000281         1253 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.291, whole genome shotgun sequence.
ACCESSION   DAGCWU010000281 DAGCWU010000000
VERSION     DAGCWU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.291"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(141..440)
                     /locus_tag="MBZ71_002773"
     CDS             complement(141..440)
                     /locus_tag="MBZ71_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBT4430663.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            791..>1253
                     /locus_tag="MBZ71_002774"
     CDS             791..>1253
                     /locus_tag="MBZ71_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBT4430664.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFST"
BASE COUNT          422 a          205 c          174 g          452 t
ORIGIN      
        1 aaaggataaa atacaatata aagaaacatc tgtttcttta tttagaataa aaaaatacag
       61 actctgttca aataaatgaa cctagcctgc attatgagat tgactatact ttttaattca
      121 ttgagatgaa taactcgttt ttataaatgc ccaaaagtaa tcggtgccga agccattcct
      181 cgacctaatt ctttttcaaa ttttcctatt gttgatatct ccatataaaa cggtgatact
      241 ggtttagaca attctcccct gattgctaac ggacgtaaac tacgttccaa attatttatg
      301 actttaggat aatactgacc tttttctaag cctgtctttg cgtctatcaa aagtatacgt
      361 tccttttctt tgatctctgg atttaaaacg agatcatata attttagtac catgttttct
      421 atttcttcac ttttagacat cacgatttcc tcccaaaaat atatttcttc ccactacatt
      481 ctataaacaa agtatacgct acatctagat ttttttaaat cattactgga ttgttaaaac
      541 ctttcaatac actgttatgg caacgatttt tattttaaaa acattctaaa ttaaatcagt
      601 aaaaattttt attctaagat ttttattaga attacgccta atttgtgtct ctcagggcaa
      661 ttctaagaac gtttcaagag atagcgacac tatcgttatc gaaaaaggcg aaaacctatc
      721 tcattttctg ctaatgtaag ttggtttagc ttttgttaat atgactttat gaaaatggag
      781 gtaattaaat atgatttttc ctaaagttaa tgaagcaagc ttgaataaag gatccttttt
      841 tcatatctat gaaaacgaag attttgaact gtataatatc ctttcacaaa aagaggtcat
      901 cgaaaaccaa ataatctgcc atccaattat ttacaaatct attttcaagt atgcattttt
      961 tgcttccaat gattttcaaa gaatggagat taccgactgt atattcgaaa actgtgattt
     1021 ttcaggctgt atattgacaa aaagcatgtt gcatcgcgtg aaatttgtca attgtaaatt
     1081 aacaggtact aaattgatgg aaagttatat tgggaacacg ttattcgaga attgtaaaat
     1141 ggactatgta aatttatctg gttcaaacat aaaagaaagt acctttgagc actctgtctt
     1201 atcaagcgca gattttgtag actgttccct cactaaaata atgttttcta cta
//
LOCUS       DAGCWU010000282         1202 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.292, whole genome shotgun sequence.
ACCESSION   DAGCWU010000282 DAGCWU010000000
VERSION     DAGCWU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.292"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(471..836)
                     /locus_tag="MBZ71_002775"
     CDS             complement(471..836)
                     /locus_tag="MBZ71_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430665.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(912..1157)
                     /locus_tag="MBZ71_002776"
     CDS             complement(912..1157)
                     /locus_tag="MBZ71_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4430666.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
BASE COUNT          407 a          214 c          235 g          346 t
ORIGIN      
        1 caatattaac gctagcaatg gcaggttgtt ctgactctgg cttagcaact gaactgatca
       61 caaaatcaac tgaagccacg acagaggagt taaaatgtgg aaacgatcac agtgactaca
      121 gaggcttaaa tcgacgatgc atgggaatat taggcagaag caattgtaag catcgatgta
      181 gtaatcgaag aaggtgtaga agaagcaaca gaaaaatcaa aagaaagatc aaaagaagca
      241 atctgtgctg tgcatcgtat acagacgtcg aagatgcggt acttttaaac cacgtcagga
      301 gatgttcatt tatgtctagg gaagttggcg atgaaccaaa actagagtac gcttttagtt
      361 ttgcacgtga ggatgaagat aaccctaaaa cttacgacat tttagcttga tctaagatgg
      421 ttaaagcttt ttaatgaagt aagaggagat agaaggcttt tctagttata ttaggtaaga
      481 taaattcctt tttgctccaa atttgctaaa cattctgcta gataaacttc atcgtgttcc
      541 ggataaatcg gcacgttgcg ctctacagta ggtaaatcaa aaaatgcttc gcatgatttg
      601 ccacgataca taggattttt ccactctggt gctgggctgt aaccacgttt ttccatttca
      661 tccatgacaa gctgatgata ttggaataat ctataaggag aatagtgaaa aacataatcc
      721 acagtagcat gtttctttcc ccagccattt cctcttaatg cacagcattc ccgatgttgt
      781 cctaaaagct gttgacgagg aagctttgaa atcaattttt catgccataa tcgcatagaa
      841 atgtctcctt tatatataga ttgttaacct tagacgttaa atgaaaaaac cagcaccaag
      901 gctggaaaaa atcacttcag tttgcttgct gtttctgagc gaccaattag gtaatcaacg
      961 gaaatatcgt aaaagtcagc gatatagatt aattcaatga tgcttggaat ttcttctcct
     1021 tcttcgtact tacgatattt gaataaagga atacttaacg cgctagaaac agctacttga
     1081 gataaagatc ggccagttct taagaaattc agacgacaac gtaaagtatc cagtaaaata
     1141 gcttcttcga tactcattta atcacctttc attatagtag aatagttata atgactatag
     1201 aa
//
LOCUS       DAGCWU010000283         1181 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.293, whole genome shotgun sequence.
ACCESSION   DAGCWU010000283 DAGCWU010000000
VERSION     DAGCWU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.293"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            121..1161
                     /locus_tag="MBZ71_002777"
     CDS             121..1161
                     /locus_tag="MBZ71_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBT4430667.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          481 a          171 c          199 g          330 t
ORIGIN      
        1 aggtagtatt taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta
       61 ttttcgaaaa gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca
      121 atgactgatt ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt
      181 ccaaaagtct ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc
      241 tatatgcttt taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa
      301 ggtaatgttt attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa
      361 caaaaagcga tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag
      421 atgggattta acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta
      481 aaagtctcag aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac
      541 gttgataaat cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa
      601 tcccttgggg ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag
      661 ggtatgaaaa tcataccaga acttaataat aatatattag acactaatag acacaatata
      721 gacactgaaa aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg
      781 acaaacgaca gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt
      841 tcttcaagtt acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa
      901 gctgaaatag aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat
      961 gcagaacaag aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa
     1021 gtcgaaaata tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca
     1081 atagctgcta aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc aagcactatt
     1141 acgaaagact ggttagagtg aaactttact gacaaagtga t
//
LOCUS       DAGCWU010000284         1174 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.294, whole genome shotgun sequence.
ACCESSION   DAGCWU010000284 DAGCWU010000000
VERSION     DAGCWU010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.294"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(58..142)
                     /locus_tag="MBZ71_002778"
     tRNA            complement(58..142)
                     /locus_tag="MBZ71_002778"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Leu,seq:caa)
     gene            complement(163..233)
                     /locus_tag="MBZ71_002779"
     tRNA            complement(163..233)
                     /locus_tag="MBZ71_002779"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Cys,seq:gca)
     gene            complement(279..350)
                     /locus_tag="MBZ71_002780"
     tRNA            complement(279..350)
                     /locus_tag="MBZ71_002780"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Gln,seq:ttg)
     gene            complement(362..434)
                     /locus_tag="MBZ71_002781"
     tRNA            complement(362..434)
                     /locus_tag="MBZ71_002781"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(399..401),aa:His,seq:gtg)
     gene            complement(437..510)
                     /locus_tag="MBZ71_002782"
     tRNA            complement(437..510)
                     /locus_tag="MBZ71_002782"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Trp,seq:cca)
     gene            complement(517..597)
                     /locus_tag="MBZ71_002783"
     tRNA            complement(517..597)
                     /locus_tag="MBZ71_002783"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(561..563),aa:Tyr,seq:gta)
     gene            complement(599..674)
                     /locus_tag="MBZ71_002784"
     tRNA            complement(599..674)
                     /locus_tag="MBZ71_002784"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Phe,seq:gaa)
     gene            complement(710..782)
                     /locus_tag="MBZ71_002785"
     tRNA            complement(710..782)
                     /locus_tag="MBZ71_002785"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(747..749),aa:Asp,seq:gtc)
     gene            complement(792..864)
                     /locus_tag="MBZ71_002786"
     tRNA            complement(792..864)
                     /locus_tag="MBZ71_002786"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Val,seq:tac)
     gene            complement(880..951)
                     /locus_tag="MBZ71_002787"
     tRNA            complement(880..951)
                     /locus_tag="MBZ71_002787"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(916..918),aa:Glu,seq:ttc)
     gene            complement(966..1054)
                     /locus_tag="MBZ71_002788"
     tRNA            complement(966..1054)
                     /locus_tag="MBZ71_002788"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1018..1020),aa:Ser,seq:gga)
     gene            complement(1094..1167)
                     /locus_tag="MBZ71_002789"
     tRNA            complement(1094..1167)
                     /locus_tag="MBZ71_002789"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1131..1133),aa:Asn,seq:gtt)
BASE COUNT          336 a          307 c          264 g          267 t
ORIGIN      
        1 ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac
       61 cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt
      121 ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg
      181 aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac
      241 tattatcaat ctacaaaata aagaataaat tatagaaact ggggtagctg gattcgaacc
      301 aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaaaa
      361 gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac
      421 ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac ggttttggag
      481 accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga ttcgaaccac
      541 tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta ctcctccatg
      601 gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg ctctaccaac
      661 tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac ggttccgacg
      721 ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa
      781 ccagttcatt atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg
      841 ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg ggattcgaac
      901 ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc caggtaaatt
      961 tgctacggag agtaagggat tcgaaccctt gagacagttt gcaccatcta catgatttcc
     1021 aatcatgctc cttcggcctc tcggacaact ctccagagtg aaagaagctc aatactcttt
     1081 ctcaaaatga gaactccggc agtaggactc gaacctacga catcatgatt aacagtcatg
     1141 cgctactacc aactgagcta tgccggaata acat
//
LOCUS       DAGCWU010000285         1166 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.295, whole genome shotgun sequence.
ACCESSION   DAGCWU010000285 DAGCWU010000000
VERSION     DAGCWU010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.295"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            46..261
                     /locus_tag="MBZ71_002790"
     CDS             46..261
                     /locus_tag="MBZ71_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4430668.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            298..660
                     /locus_tag="MBZ71_002791"
     CDS             298..660
                     /locus_tag="MBZ71_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBT4430669.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            706..1032
                     /locus_tag="MBZ71_002792"
     CDS             706..1032
                     /locus_tag="MBZ71_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430670.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT          445 a          138 c          250 g          333 t
ORIGIN      
        1 aaatatatca tattgttgta tataataaaa caggagggga gaatcatgaa aaaagtaagt
       61 aatctcagcc gatacagaaa agaaaaagga ctttctcaaa cggagttagc aaaaaaaatg
      121 aatgttacac agcaatgtgt aagttcgtgg caaacaggga gaacgattcc taaaccctat
      181 caaatgaaga tgctttctga gatattaagt gtaccaatga atgagctgtt ttctgatgta
      241 tttaataaag taaacagtta actaataagc aattgaatga ttttaggagg agaagacatg
      301 tattgtagaa aatgtggcaa agaaataaca gatgaaagta agtattgcca gttttgtgga
      361 gcactacaag aaacagatga gacaaccgga acagacacag taaagaaaag tactgagcag
      421 acaattaatg aaatgaagga acaaatcgta catggacaag agacagggaa gctgtatttg
      481 attattggtt ggatttctat ggtagtatcg ttagcattta ttcctgttgt atttggtgct
      541 gttgctgtta ttatgggcta cttatatcga gaaaaagatg agaaaatggg tacgattttg
      601 atgatagcag gggtagctgg agcaatcttt ggtgtacttt tagggatgtc aatgtactga
      661 taagagggat aagaacaaat tattttggag ataggagata aaaagttgaa taaagtaaat
      721 atgaacaaga agtttgtcat tataggtatt gttgtcttgg ctattatagg tattattttt
      781 tcaaaaatga caggtacatc tggattagag aagtcatcaa aaccattggt aacacagatt
      841 ttacaagaac agtacaactt aaatcgatca tgtgatgatg tgacgattac tcatgaaaat
      901 ggagataaca cataccgggc taaagctata ttagacaatg gaagtgctat caacataaat
      961 attgagtact atccaaagaa agaccgtata tatgtggaga taccatatgc tgaggtgtta
     1021 atgttaaatt gatttaaatg tattatgcat accgtccaaa caggaactcc caaaggtgta
     1081 aattcactag ggagttttta tagtttccta taaattaaat actaatacag gtgacaaaaa
     1141 aatattacaa tgattgtcgg gaattt
//
LOCUS       DAGCWU010000286         1141 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.297, whole genome shotgun sequence.
ACCESSION   DAGCWU010000286 DAGCWU010000000
VERSION     DAGCWU010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.297"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            50..331
                     /locus_tag="MBZ71_002793"
                     /pseudo
     CDS             50..331
                     /locus_tag="MBZ71_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            674..930
                     /locus_tag="MBZ71_002794"
                     /pseudo
     CDS             674..930
                     /locus_tag="MBZ71_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          398 a          174 c          206 g          363 t
ORIGIN      
        1 aacatgttta gacattttcc tctaggaaaa aattaactag caccacgggt ataaacgtaa
       61 tcccttcgta tctgaatatg ttaaacgact tgcaaaaggt atttgtcaac tttgccaaga
      121 aaaagggcca tttataaaag acggtgtacc ttatttacat tgtcatcata tagaatattt
      181 gtcgcaaggt ggaaaagatg tgattgaaaa ctgtatcgcg ttatgtccca attgtcatgc
      241 tagaattcat gaacttgagt tacctgacga taaagaaaaa ttgctgaaga tcgttgaaaa
      301 aagagaaaat agcttttcta aaataccgta atttattttt ataggacaaa taagctaatg
      361 tgagctatgc cttctatata agactaagtt ctattataag taacgaagta attctcgttc
      421 cttcctgtag taatattttt taaataacct ataggcttct tcaaaagtac atcctccagt
      481 aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa aaagtagttg
      541 gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg atcatttact
      601 acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt tctaaaatca
      661 catcaggaaa tgcatgagat tctttaagaa aggcaattat agggctatga ttacggttcg
      721 tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg ggattacttt
      781 atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca atcgtcaaac
      841 ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat tcatggaaga
      901 atccttaatg aatttggtgg gctggaatga cctgccgttt aaaattcacg cccgaaattc
      961 agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa tcaattcgtt
     1021 gttaccgcta ttcaagtagg gcaccgaaga aatgtctatg attaagagac tgtttatttt
     1081 tacaaaaaaa taataactgg aatctagtta aaactcttga agtgagagat ttgactagat
     1141 t
//
LOCUS       DAGCWU010000287         1134 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.298, whole genome shotgun sequence.
ACCESSION   DAGCWU010000287 DAGCWU010000000
VERSION     DAGCWU010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.298"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            55..>1134
                     /locus_tag="MBZ71_002795"
     CDS             join(55..331,331..>1134)
                     /locus_tag="MBZ71_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4430671.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          425 a          192 c          217 g          300 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
      961 ataaaggctg tccttttgat aatgccgtag ctgaatcaac ttataagtcg ttgaaagtag
     1021 aatttgtcta tcaatacaca tttgaaacct tacaacaatt ggatttggag ttatttgact
     1081 atgtcaattg gtggaaccac cttcggttgc acggtacact tggctacgag acac
//
LOCUS       DAGCWU010000288         1084 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.299, whole genome shotgun sequence.
ACCESSION   DAGCWU010000288 DAGCWU010000000
VERSION     DAGCWU010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.299"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            195..>1084
                     /gene="aph(2'')"
                     /locus_tag="MBZ71_002796"
     CDS             195..>1084
                     /gene="aph(2'')"
                     /locus_tag="MBZ71_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021401024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ie/IVa family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBT4430672.1"
                     /translation="MTTYTFDQVEEAIEQLYPDFTINTIEISGEGNDCIAYEINGNFI
                     FKFPKHSRASINLLNEVTVLKTIHNELSLPIPEVVFTGMPSEMCQMSFAGFTKIKGVP
                     LTPLLLKNLPKQSQDQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKL
                     LSRELKGHQMKKVDDFYRDILDNEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDF
                     GDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYMMKEKYWSFEKI
                     IYGKEYGYMDWYEEGLNEIR"
BASE COUNT          410 a          157 c          204 g          313 t
ORIGIN      
        1 aaatggccat tagaagatag gaacttagca gatactgcac tttggctaaa tgcagaattt
       61 atgaaaaatg cagcagagat aggttatgca agatttttat atggaaatag catgaaacaa
      121 aaatgaatta actataattg ctaaattctt atttacagtc aatcagattt ttgattaaag
      181 ggagtagaga agacatgaca acttatactt tcgaccaggt agaagaggca atagagcagt
      241 tatatcctga ttttactatc aatacaatag agatttcagg agaaggcaat gactgtattg
      301 catatgaaat aaacgggaat tttattttta aatttccaaa gcattcaaga gcttcgatta
      361 atctcttgaa tgaagtaacc gtactcaaaa caatccacaa tgaattatca ctacccattc
      421 ccgaggtggt ttttacagga atgccatcag aaatgtgcca aatgtctttc gcaggtttta
      481 caaaaattaa aggagtacct ttgacacctc ttctactcaa aaatctgccg aagcaatctc
      541 aagatcaggc agctaaggac ctggcccgat ttttaagtga acttcacagc ataaatatct
      601 ctggattcaa aagtaatctg gtattagatt ttcgagagaa gataaatgaa gataataaaa
      661 aaatcaaaaa gttactatcc agggaattaa agggtcacca gatgaagaaa gtggatgatt
      721 tttacaggga tattctagac aacgaaatct acttcaaata ctatccttgt cttattcata
      781 acgatttcag cagcgatcat attttatttg ataccgaaaa aaataccatt tgtggaataa
      841 tcgattttgg agatgcagct atttctgatc ccgacaatga ttttataagt ttgatggaag
      901 atgatgaaga gtacggcatg gaattcgtat caaaaatatt gaaccattac aaacataagg
      961 atataccgac agttttggaa aaatatatga tgaaagaaaa atactggtcg ttcgaaaaga
     1021 ttatctatgg aaaggaatat ggttatatgg attggtatga agagggatta aatgaaatca
     1081 gaag
//
LOCUS       DAGCWU010000289         1070 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.300, whole genome shotgun sequence.
ACCESSION   DAGCWU010000289 DAGCWU010000000
VERSION     DAGCWU010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.300"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            25..>1070
                     /locus_tag="MBZ71_002797"
     CDS             25..>1070
                     /locus_tag="MBZ71_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4430673.1"
                     /translation="MTELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNII
                     DTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINKID
                     QNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKY
                     MSGKSLEALELEQEESIRFQNCSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKS
                     ELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKI
                     DKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLD
                     ALLEISDSDPLLQY"
BASE COUNT          386 a          169 c          223 g          292 t
ORIGIN      
        1 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
       61 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      121 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      181 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      241 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      301 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      361 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
      421 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
      481 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
      541 caagaggaaa gcataagatt tcagaattgc tccttgtacc ctgtttatca tggaagcgca
      601 aaaagcaaca tagggattga gcagcttata gaagtgataa cgaataaatt ttattcatca
      661 acatacagaa agaagtctga actttgcgga aatgtcttca aaattgaata ttcggaagaa
      721 agacaacgtc ttgcatatgt acgcctttat ggcggaatcc tgcatttgcg ggattcggtt
      781 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
      841 ttatgtaaaa ttgataaggc ttattccggg gaaattgtta ttttgcaaaa tgagtttttg
      901 aagctaaata gtgttcttgg agatacaaag ctattgccac agagagagag aattgaaaat
      961 ccgctccctc tgctgcaaac aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta
     1021 cttgatgcac ttttagaaat ctccgacagt gacccgcttc tacaatatta
//
LOCUS       DAGCWU010000290         1062 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.301, whole genome shotgun sequence.
ACCESSION   DAGCWU010000290 DAGCWU010000000
VERSION     DAGCWU010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.301"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(37..993)
                     /locus_tag="MBZ71_002798"
     CDS             complement(37..993)
                     /locus_tag="MBZ71_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430674.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAGCWU010000291         1058 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.302, whole genome shotgun sequence.
ACCESSION   DAGCWU010000291 DAGCWU010000000
VERSION     DAGCWU010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.302"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(215..919)
                     /locus_tag="MBZ71_002799"
     CDS             complement(215..919)
                     /locus_tag="MBZ71_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430675.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          303 a          190 c          194 g          371 t
ORIGIN      
        1 gttaggcatc ttaagaaatc aggcaactcc aagatatctt caggtagaag agaagtcgcc
       61 taacattgta ggatcatagg tgatttgttg gacaggtcaa gctgatattt gagcctgatg
      121 ttagaaggaa tttcttttta cttgctgtag aaattattat atgactaaaa aggctgggat
      181 tttaaatccc agccttttat gacaaataga atttttatcg atcagtaatc gctgtcgctg
      241 caaattgttc gatcaaagga atcaaagtca atttgacttt gtatttccgt tcgagtgctt
      301 tttcgataac gaactcattt tcagtagtca tagaaatagt tgctaattta cgaggttcat
      361 cagcattttt ttcattaata actacttcat gttcattttg ttctgtgtaa aatttaggat
      421 actcagcaac ggcttttgaa aaatcagcaa aacttaaagc ggcattcgta tcagttgagc
      481 gtgtattttc agttgtgtcc gcttttggtt tttccgtttt ttcttctggc acactgactg
      541 tttcttctgt aactggtgtc gcaacagctt gcgcagttgg ttcatcgttt ttcaatcgtt
      601 gatcattcca gtaagacacg atttcaataa gaagattgtc acggaatcgt tcgaaaatcc
      661 cgttttctcc tgaacgaatt ctctttaata cagtattgaa gttgtcatcg attggcacgt
      721 tgatccgtgt atcttcattt gttttcccag ctaatccgat cgttaagatt tgacggtttt
      781 tgggatcaag catgacttgt aacggcaaac cataacgtcg caagctctct tcttctagag
      841 aacggaataa ttgtcctctt acaccatcca atgaaggacg gaattgattt aataattttt
      901 gtttctgttt tttcttcata tgttctctcc ttcgctcata ccctttcgat tataagaaaa
      961 aaacagccaa atgaaaagga gtaatgcaaa aaaacttgcg tatacgcaaa ttacaacaga
     1021 aaaatttgcg tattttcccg ttttttgaaa gaaaaata
//
LOCUS       DAGCWU010000292         1029 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.303, whole genome shotgun sequence.
ACCESSION   DAGCWU010000292 DAGCWU010000000
VERSION     DAGCWU010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.303"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..886
                     /locus_tag="MBZ71_002800"
     CDS             <1..886
                     /locus_tag="MBZ71_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HBT4430676.1"
                     /translation="INRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDG
                     KYLYSIYKDQDSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKP
                     QIGSVFSARLKLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNE
                     DFWSSLDVNYSKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFED
                     TQITMLENKSVKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELK
                     KDGRVYGYINSEAAVEMN"
BASE COUNT          393 a          123 c          187 g          326 t
ORIGIN      
        1 aataaacaga tatttaactt ctaaagatag tggtcaacca acttggggaa atctagagtg
       61 tactataagt aaaggacaaa ctacaaaaga taaaatatat tttgttaaag gagcatataa
      121 tttaggggat ggaaagtatc tatattctat ttataaagat caggattcct ctgaatggtt
      181 agggtattta aaagcacatg ttagtttagc ctatactcct attgaagaaa ttaataaaaa
      241 cgtaactgtt actaaaaatt ggtcgatttg gaataatcta caatgggaaa aggaaattga
      301 aaagcctcag attggttctg tattttcagc tagattaaag ttaaccaatg tatcgaataa
      361 tgctgtctat tacaagcttt ataaatctgg aaaattttat ggttatatca atgctgaggc
      421 tgtcaaagac cttacaacaa caaagcttaa taagtatgtt acatttagtg ttaataatga
      481 agatttttgg tcttctttag atgtaaatta ttcaaaagga aaaactgaaa gaggccgagt
      541 attttacatt agtatttcat ataataccgc agataaccaa ccgatttatt cagtatatac
      601 agatgaaaca tgtacggaat ggcgagggta ttataaggga aacaactttg aagacactca
      661 aattactatg cttgagaata aaagcgtgaa agtaacaaaa agtgggtata cagtatgggg
      721 agacttgaat ttttggacta agtcaggaat atcgaacaca ggagatatat acaccactga
      781 ccgaaaattt tataatttta ccaacaatgc ttactattat gaattgaaaa aagatgggag
      841 ggtctatggc tatataaata gtgaagcagc tgtagagatg aactaaaata ttgctgtccc
      901 ttaatcttat ggggcagcaa tatttttata ttattaatca tttttctgaa gcgagggtag
      961 aagaattaat caatagtttt tcttgttttg taatataata gtttaattac caaaaattat
     1021 ttaagtaaa
//
LOCUS       DAGCWU010000293          976 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.304, whole genome shotgun sequence.
ACCESSION   DAGCWU010000293 DAGCWU010000000
VERSION     DAGCWU010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.304"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(6..200)
                     /locus_tag="MBZ71_002801"
     CDS             complement(6..200)
                     /locus_tag="MBZ71_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430677.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(300..386)
                     /locus_tag="MBZ71_002802"
                     /pseudo
     CDS             complement(300..386)
                     /locus_tag="MBZ71_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          343 a          151 c          127 g          355 t
ORIGIN      
        1 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
       61 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      121 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      181 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      241 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      301 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      361 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      421 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
      481 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
      541 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
      601 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
      661 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
      721 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
      781 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
      841 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
      901 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
      961 ggtagtatct aattta
//
LOCUS       DAGCWU010000294          926 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.306, whole genome shotgun sequence.
ACCESSION   DAGCWU010000294 DAGCWU010000000
VERSION     DAGCWU010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.306"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            1..797
                     /locus_tag="MBZ71_002803"
                     /pseudo
     CDS             1..797
                     /locus_tag="MBZ71_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          328 a          169 c          157 g          272 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa gtaaatgcat
       61 ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct acttatattc
      121 ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt atacgagaag
      181 acggcactaa agaagtacta agttatgcga ttgctccgac tgaatcgacc tacgtttgga
      241 atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg tttattaccg
      301 atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct aaataccaac
      361 attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag
      421 aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag gcaaatacct
      481 ttttagggag tatgattgag aagtagcaaa aaacttatcc taaagtgacg cagtcactga
      541 taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc cgaagcattt
      601 actcaactaa tttaatcgaa tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag
      661 agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt gatacataca
      721 atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat acattagctt
      781 caatgtttac tgagtaaccc attattttgc agaaggacga gttatttaca caaaattatt
      841 gacgctccct ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt
      901 attcttgata cataaatgag aaaata
//
LOCUS       DAGCWU010000295          915 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.307, whole genome shotgun sequence.
ACCESSION   DAGCWU010000295 DAGCWU010000000
VERSION     DAGCWU010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.307"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            427..>915
                     /locus_tag="MBZ71_002804"
     CDS             427..>915
                     /locus_tag="MBZ71_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBT4430678.1"
                     /translation="MNDFTTEIVQTLVTKGGLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT          354 a          152 c          130 g          279 t
ORIGIN      
        1 attgtatttc tgctttcaaa aagttctaat attgaaagta taattccaca aatgcctata
       61 ataagtatgg gaataagatt agaaaataac accccgagcc aatctgtgaa aattactata
      121 tttactaata tccctattaa catacctatc atatttatta aatcaatttt tttcattaga
      181 tatttctcct ttattaaatc tgaagtactt atattaaaat aattctaaac tatttacctc
      241 aaaaagtgca ataaattatt tctgaacaat aaaatgatag tcaaaacaaa aaaatattgt
      301 taatcgttca gagccatccg agagtgtaaa atattttgtg taaatgaaaa aatccataca
      361 aaaaaggaag gcgcttctgt agaataaagt taacgacaac caattcacag aaaagaggac
      421 ttccctatga atgattttac tacagaaatt gtgcaaactc tagtcactaa aggcggttta
      481 aatgaattat tccgttcgca cttagaaaaa gcgataaaca cactcctacg gactgaatta
      541 acggcttttt tagattacga aaaatatgat cgcactggtt ttaattcagg taattcgaga
      601 aacggttctt actttcgatc aatcaaaacc gaatatggtg aattaacatt ggaaatacct
      661 agagatcgta atggtgagtt taaacaacaa actttaccag cctacaaaag aacaaacgat
      721 acattggaaa ccactattat ccatttattc gaaaaaggtg ttacgatgtc tgaaattgct
      781 gatttgatcg aaaaaatgta cggtcatcac tatactccac aaaccatgtc caacatgact
      841 aaagttctga ctgaagaagt aaatgccttt aaatccagag ccttaaatga taagtatgtc
      901 gctattttta tggac
//
LOCUS       DAGCWU010000296          894 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.308, whole genome shotgun sequence.
ACCESSION   DAGCWU010000296 DAGCWU010000000
VERSION     DAGCWU010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.308"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>894
                     /locus_tag="MBZ71_002805"
     CDS             <1..>894
                     /locus_tag="MBZ71_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4430679.1"
                     /translation="VSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKD
                     GTRFSQEYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGI
                     IGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSE
                     SPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQ
                     QTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTN
                     GFSIFNYNFYAAGLNLKTARLL"
BASE COUNT          344 a          112 c          152 g          286 t
ORIGIN      
        1 gttagcaatt tagaagatct tttttatcaa aatagaagag aactaaattc taagataaaa
       61 aagttgtttt tggagacagc agttgacaaa attgatattc aaaataaaaa gattatttgt
      121 gtaaaaaaag atggcacaag attttcccaa gaatatgaca aacttatact agctacaggt
      181 tcaaagcaaa ttatgttaga tgtaccgggg aataatttag ctaatatttt ttcgataaag
      241 aattttccaa atgctaaaga gatttttaaa gctttagatg ataatactat taaaaaagta
      301 ggaattattg gagcaggata tattggagtt gaaattgctg aagccataag aaaaagaggg
      361 aaagaagtat atcttttcga tgttgcagat agagtgttgt cgacttatta cgaccgtagt
      421 ttttcagata aagttgaaga aatactatca aaaaatggaa tacatctttg tctatcagaa
      481 agtcctaaaa aatatttagg aagaaaaaag attgaaaaaa tagtcactac aagtggtacc
      541 caatactcta tggatatggt cgtacaagct ataggatttc tatctaatag tccaattggc
      601 aaaaaggaac tcttgagaga tgttagtgga gcatatctaa ctaatgaata ccagcaaaca
      661 aatataaaag atatatatgc tattggggat tgtgctacaa ctttttttac gtccattaat
      721 agaagtagtg ttgatttttc tatttctaat gctctaagaa cagcttatat tgcaactcaa
      781 catattgaca atcaagattt tacaccttct ggttcccaat ttacaaatgg cttttctata
      841 tttaattaca atttttacgc agcaggtttg aatttaaaaa cagcacgatt atta
//
LOCUS       DAGCWU010000297          893 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.309, whole genome shotgun sequence.
ACCESSION   DAGCWU010000297 DAGCWU010000000
VERSION     DAGCWU010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.309"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            64..833
                     /locus_tag="MBZ71_002806"
                     /pseudo
     CDS             64..833
                     /locus_tag="MBZ71_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340391.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          360 a           99 c          159 g          275 t
ORIGIN      
        1 tttttttcga tttttcaagt tatctaatgc aattatattg atatcattag gagctgcatt
       61 gttatgaatc caacattgat tactagaaag aattactaaa aaaattagat atttctactg
      121 gtgtattagc tgatttaatt agaaatggca tgccaaaaga gggagaaatg tttaatcttg
      181 ataagattat tacatggcga gaaaattggt ctaaaaacat attaggtgag ttagaagttg
      241 gtagagtata tactaacaaa gaaatttcag agaagtttaa atgtttcaaa cagggtggaa
      301 tgagaagatc tcatcaaact aatactcttg tactgtttag tgatcaaaca ggaagtaatg
      361 tttataaaga taagtggtta aatggtattt tacaatatac cggtatgggg ctaaaagggg
      421 atcaagtatt ggataagaat caaaataaag ttttagccaa ttcaaaaagt aattttgtaa
      481 agattcattt atttgaaaca tttaaaccaa aagaacacac gtatctggga gaagtatatt
      541 tagcaggaca aatatatact gttaatgaaa aagatagttc gggcaacagc agaaaagtat
      601 ataaatttcc attagcttta ataaatcaag agcaattgat tgaagataaa gatatatata
      661 accaggaaga aaatcaaact agacatattc gtaatttatc tgatgctaaa ttagaggaag
      721 aagcaagaaa agttagtaat tataataaaa aaatggctgc aaaatatgtt aacgaagagg
      781 agttttgctt ctcaaaaaca aaaatatata aacgtacccg tggtgctagt taattttaat
      841 atcctaaaga atatctcaaa gttccttcaa aacgtcgttg tgctcgactt aat
//
LOCUS       DAGCWU010000298          871 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.310, whole genome shotgun sequence.
ACCESSION   DAGCWU010000298 DAGCWU010000000
VERSION     DAGCWU010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.310"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            6..>871
                     /gene="bsh"
                     /locus_tag="MBZ71_002807"
     CDS             6..>871
                     /gene="bsh"
                     /locus_tag="MBZ71_002807"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBT4430680.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRT"
BASE COUNT          295 a          117 c          158 g          301 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat a
//
LOCUS       DAGCWU010000299          870 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.311, whole genome shotgun sequence.
ACCESSION   DAGCWU010000299 DAGCWU010000000
VERSION     DAGCWU010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.311"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..236)
                     /locus_tag="MBZ71_002808"
     CDS             complement(<1..236)
                     /locus_tag="MBZ71_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430681.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIR"
     gene            complement(400..723)
                     /locus_tag="MBZ71_002809"
     CDS             complement(400..723)
                     /locus_tag="MBZ71_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430682.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          372 a          139 c          131 g          228 t
ORIGIN      
        1 cgtatcaaat aaagaatgac gatcaataaa gacaaaacca gtcctgataa ttcttctata
       61 aaaacactat cgggttcaaa gtagttgtaa ttaaaaactt tccacataaa gtagatagcc
      121 ggaagagtca aaatagctaa ataaaaccgt tctttgttcc aattcataag ataaacaata
      181 cttgcgacca gtgcgaccgc aggcacccaa aacaaaactc tcaccaacgt aatcatgtaa
      241 cggactcctc aacgattcat ataataaaag ataacatgtt attgcataca tcacaaatga
      301 aatgctagga aaataaaaga agaatagatc aatgaaaaat gaaaaagcag aacagttttc
      361 tgctgataag caggaactgt tccgctgaat gtgtcttatc tatcgagaaa aaatcaatgg
      421 attgatatct aagtaattca atacatataa aaagaggacg atcgcattca ctaaaaagga
      481 aagaatgatg atcccatagc gaatggaaac atatttaaca aatacttttg tttgaatacg
      541 ggcatatctt tttcctttat agctgagcat catgacaaga agcaaaaggg taatcacacc
      601 ttgaaaaatc attccaataa acagaattcc tgaggtttga taatttaatt ggagaaaata
      661 agctgctgcc gcaactaaat cgaataaaat cgcaataaat ggcaaaataa tcactcctaa
      721 cattgagaat aaataaaaga ataacatttt ttctaaaata agcaaataaa aattaaagta
      781 acaaaatagt tatgacacaa gtaaaagaaa taagaataac aatattattt tacttatatt
      841 ttgaaacttg tgagagtttg tcattcgtgc
//
LOCUS       DAGCWU010000300          851 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.312, whole genome shotgun sequence.
ACCESSION   DAGCWU010000300 DAGCWU010000000
VERSION     DAGCWU010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.312"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>851)
                     /locus_tag="MBZ71_002810"
     rRNA            complement(<1..>851)
                     /locus_tag="MBZ71_002810"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          271 c          188 g          217 t
ORIGIN      
        1 catctcacga cacgagctga cgacaaccat gcaccacctg tcactttgcc cccgaagggg
       61 aagctctatc tctagagtgg tcaaaggatg tcaagacctg gtaaggttct tcgcgttgct
      121 tcgaattaaa ccacatgctc caccgcttgt gcgggccccc gtcaattcct ttgagtttca
      181 accttgcggt cgtactcccc aggcggagtg cttaatgcgt tagctgcagc actgaagggc
      241 ggaaaccctc caacacttag cactcatcgt ttacggcgtg gactaccagg gtatctaatc
      301 ctgtttgctc cccacgcttt cgagcctcag cgtcagttac agaccagaga gccgccttcg
      361 ccactggtgt tcctccatat atctacgcat ttcaccgcta cacatggaat tccactctcc
      421 tcttctgcac tcaagtctcc cagtttccaa tgaccctccc cggttgagcc gggggctttc
      481 acatcagact taagaaaccg cctgcgctcg ctttacgccc aataaatccg gacaacgctt
      541 gccacctacg tattaccgcg gctgctggca cgtagttagc cgtggctttc tggttagata
      601 ccgtcaaggg atgaacagtt actctcatcc ttgttcttct ctaacaacag agttttacga
      661 tccgaaaacc ttcttcactc acgcggcgtt gctcggtcag actttcgtcc attgccgaag
      721 attccctact gctgcctccc gtaggagttt gggccgtgtc tcagtcccaa tgtggccgat
      781 caccctctca ggtcggctat gcatcgtggc cttggtgagc cgttacctca ccaactagct
      841 aatgcaccgc g
//
LOCUS       DAGCWU010000301          849 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.313, whole genome shotgun sequence.
ACCESSION   DAGCWU010000301 DAGCWU010000000
VERSION     DAGCWU010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.313"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            94..>849
                     /locus_tag="MBZ71_002811"
     CDS             94..>849
                     /locus_tag="MBZ71_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4430683.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYV"
BASE COUNT          320 a          106 c          155 g          268 t
ORIGIN      
        1 tttttatgtt tattaacttc ggaagactaa aattgttact ggtaatgata catttgtaaa
       61 aataaaaaag atgattttta ggaagtgaag agagtgcata aaattagtgt tatagtaccc
      121 gcatataacg tagaaaacta tttagaaaac tgcttagaat ctatttccaa tcagacacat
      181 tcaaatattg aaataatagt agtcgatgat ggttccccgg atcgaagtgg ggaaattgca
      241 gaagaatata aaaagaaaga tatgcgatta aaagtgatac atcaagtgaa tggtggttta
      301 agtgcggcta ggaattctgg tcttgattct gcaacaggag attttgtgtg ctttattgac
      361 agcgatgatt ggatagaacc agactatctt gagttactat attttggtac gcaaacttat
      421 ggagcagata tctcggtgct aaaaataaaa aagatttcaa gtttagaaca actaaaagaa
      481 catacaattg ctgagagaaa atgggaaata tttgatagag aacaagcaat gaaaaactta
      541 tttataaata ataaaattgg ctattctgca aataataaat tattcaatag aaatttattt
      601 gataatataa gattcccact gggtaaatta atggaagata aggctactac ttatttgttg
      661 atcgatcatt ccgaaaaagt tgctgtcaat tcatctgcta aatatcacta ttaccaaagc
      721 cctaaaagta tactgagagg aagcttcaat cctagaattt ttgattcttt tgaagtccat
      781 gaagatatac tagtattcat agataagttt tatccggaat tatcaattaa tgttagaact
      841 cgctatgtc
//
LOCUS       DAGCWU010000302          826 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.314, whole genome shotgun sequence.
ACCESSION   DAGCWU010000302 DAGCWU010000000
VERSION     DAGCWU010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.314"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..190
                     /locus_tag="MBZ71_002812"
     CDS             <1..190
                     /locus_tag="MBZ71_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBT4430684.1"
                     /translation="IEARPVWKPMHLQPVFKNYDYIGDDVAETLFDLGVCLPSDTKMT
                     DEDLKYVVSTIKRVINDA"
     gene            180..800
                     /locus_tag="MBZ71_002813"
     CDS             180..800
                     /locus_tag="MBZ71_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBT4430685.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNTPEKFKGS"
BASE COUNT          306 a          113 c          153 g          254 t
ORIGIN      
        1 tatagaagcc cgcccagtat ggaaaccaat gcatttacaa cctgttttca aaaattatga
       61 ttatattggc gatgatgtag cagaaacttt atttgactta ggagtatgtt taccatcaga
      121 tacaaagatg acagatgaag atttaaaata tgtagtatct acgattaaaa gagtgatcaa
      181 tgatgcgtaa aaaaactttc tatcagcttt ttgggaaaag aattttagat atcctattgt
      241 caggaattgc tctcatcgtt ttatcgccga taatcttaat tgtaggtttt ttagtaagaa
      301 ttaaacttgg ttctccaatt atcttcaaac aagaacgacc aggaaaaagt gaaaaaattt
      361 tttccatgta taaatttcgt acaatgactg acgaaagaga ccacaatgga gaatatttac
      421 ctgatgaaat tcgactaact aaatttggga aaatgcttag agcaacaagt ctggatgagc
      481 tacccgaact ttggaatatt ttgaaaggtg atatgtctat cgtaggacct cgtccattat
      541 tagtggagta tttgccacta tatagtgaga agcaacggaa aagacacaac gtaagaccag
      601 gattaactgg actggctcag gtcaatggaa gaaatgctat tagttgggaa gaaaagtttg
      661 atttagatgt ttattatgtt gataaaatta gtttctttaa tgatcttatt attataattc
      721 agacatgtaa aaaagtaatc aagaaagaga atattaatac aatcaataac aataccccag
      781 aaaaatttaa agggagttaa atacagcagc ttaatgcaag ttttgt
//
LOCUS       DAGCWU010000303          790 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.316, whole genome shotgun sequence.
ACCESSION   DAGCWU010000303 DAGCWU010000000
VERSION     DAGCWU010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.316"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..782
                     /locus_tag="MBZ71_002814"
     CDS             <1..782
                     /locus_tag="MBZ71_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4430686.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc
//
LOCUS       DAGCWU010000304          774 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.317, whole genome shotgun sequence.
ACCESSION   DAGCWU010000304 DAGCWU010000000
VERSION     DAGCWU010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.317"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(248..727)
                     /locus_tag="MBZ71_002815"
     CDS             complement(248..727)
                     /locus_tag="MBZ71_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430687.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          319 t
ORIGIN      
        1 gacgctccct taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat
       61 tagatatgaa aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag
      121 atgagagtca ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg
      181 atataggaag tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat
      241 ttttttgtta tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta
      301 tatcttctaa attagaagat atttgtatat caatatggaa tatattacct tttttgtatt
      361 tttttatgtt atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag
      421 ctggatttag tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt
      481 tttttatttt tttacctttg taaaaatatt cgattgaata ttctatttgg cacgactcac
      541 ttggcagtat atgtatgtca atatttttca tctgtttcat aggtatttca aaatctaaac
      601 tattaaaaac atataattcc ctgtaattat ttacatctaa ctctactaaa tttatctttg
      661 gaacaataga tcctccaccc attccaccat aataaaaaaa atcttgttct ctatcaaaat
      721 agcacatatt aagctcagtt tgtttatcat cctcaataat tttataaatc aaac
//
LOCUS       DAGCWU010000305          761 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.318, whole genome shotgun sequence.
ACCESSION   DAGCWU010000305 DAGCWU010000000
VERSION     DAGCWU010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.318"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            51..731
                     /locus_tag="MBZ71_002816"
     CDS             51..731
                     /locus_tag="MBZ71_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBT4430688.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          270 a          127 c          152 g          212 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
      721 tcccagctta aatcatagat accgtaaggg attttattct t
//
LOCUS       DAGCWU010000306          742 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.319, whole genome shotgun sequence.
ACCESSION   DAGCWU010000306 DAGCWU010000000
VERSION     DAGCWU010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.319"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            162..235
                     /locus_tag="MBZ71_002817"
     tRNA            162..235
                     /locus_tag="MBZ71_002817"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:196..198,aa:Ile,seq:cat)
BASE COUNT          228 a          148 c          132 g          234 t
ORIGIN      
        1 gaggggcggt acataactat attgaaaact ataaaaatca ttcgataaaa tagtgatgtt
       61 gccgcatatc ttcactatca ctcataaata gtcacactcc aagatatgcg ataacaggtt
      121 tgttgccaca cattctactg gttgactgtt tatggctttc cgtaccctta gctcagttgg
      181 ttagagcaga cggctcataa ccgtccggtc gtaggttcga gtcctacagg gtacatactc
      241 taagtgattt aaaatgcgaa taaaatataa aaatgactcc ccttttgact cccccaagca
      301 gtatatggat gtctctaaaa gttgtttttt tagataataa aaagctgata ataagcgaaa
      361 aacacttaaa atcagcttta aaccctattc tcgaaatccg gcgaaccggc taataccggc
      421 gcgcaggttc aaatcctgta ccttccttat atcaacgatt agaggttgtg aagaagctgt
      481 cctgcatcaa gtaataagaa cggccaaaca aaaatagctt ttcctatttt ttcgtttgat
      541 tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaata aggggctgtg
      601 acaagacttt tgtcacagcc ccttattgtt gtacttttaa cctacaatat tttttgattc
      661 cccctgagta ctatttttta taaatgctag taaatcaatc ttctttggcc tttttttgat
      721 aaaaaaaata ctagatgtaa ga
//
LOCUS       DAGCWU010000307          741 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.320, whole genome shotgun sequence.
ACCESSION   DAGCWU010000307 DAGCWU010000000
VERSION     DAGCWU010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.320"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            7..708
                     /locus_tag="MBZ71_002818"
     CDS             7..708
                     /locus_tag="MBZ71_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBT4430689.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          258 a          141 c          129 g          213 t
ORIGIN      
        1 tttttaatgc gtgttaataa aatcgatttt cacttttcaa aactcagata tcaacctgat
       61 tttgaacatt tctcggatga agagttccaa cagatcaagg aacatactgt tcttcgttct
      121 tataaaaaag gacagatcct tttcgacgat ggtgatccca gacaacggtt gtatatcttg
      181 accaaaggac tcgttcgttt agaacgttat gatgaaagtg caacttatta ctactatgac
      241 tatgtgaata caaataacct ttttcctatg tcaggaatgt ttggagaaga gacctacact
      301 tatacagctc aagcgatgac tgatattgaa acattctacc ttcctgtttc ctactatgaa
      361 aaattagcac gaaaaaatcc cgaacagttg atctatatga tacaaaaaat ttctaaagta
      421 gttgaaatac atgaattgcg tatccaaatc ggattgacct ctagcgcctt tgaccgggta
      481 aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc
      541 accattcctt atccgatcac cttaaaagaa ttagcagtga atagcggcac gacacgtgaa
      601 actgctggac aagtaatcaa acaactacgt gattccgggc tgttagatta tgacaaaaag
      661 cgttttactt tctgtaagac agctactact ccagctatga gagaataacg cgaacaaaaa
      721 taagcagtga ccctgataaa t
//
LOCUS       DAGCWU010000308          728 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.321, whole genome shotgun sequence.
ACCESSION   DAGCWU010000308 DAGCWU010000000
VERSION     DAGCWU010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.321"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(266..466)
                     /locus_tag="MBZ71_002819"
     CDS             complement(266..466)
                     /locus_tag="MBZ71_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4430690.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          263 a          116 c          125 g          224 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttat
//
LOCUS       DAGCWU010000309          713 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.322, whole genome shotgun sequence.
ACCESSION   DAGCWU010000309 DAGCWU010000000
VERSION     DAGCWU010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.322"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..176)
                     /locus_tag="MBZ71_002820"
     CDS             complement(<1..176)
                     /locus_tag="MBZ71_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430691.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDR"
BASE COUNT          216 a          122 c          101 g          274 t
ORIGIN      
        1 cgatcaaatt ggtctttttt ataattcaga agagcgtgat caaaatcatt ttcgatcaaa
       61 acttttaggt attgatttcg actccatttt ttgccagtct tgaccgtaag ttcttcacaa
      121 cgtttgtcaa ttttattaat atacttttct tcaatatttc taattaataa gtccatattt
      181 tcctccaaat ttattatgtg tagggatatg tgtagctaca catatcccta cacatttttt
      241 gattttgcta cacacaaacg cttattttac tgcccacatt cgctttcatc ctacccacaa
      301 atctacacac aaatccaaaa ttttgatatc atttatcttt cttagaaacg tggtaggcac
      361 agcctaccta acacctttcg tgagaaagtg atatcacgtt tccttatgct ctttaaaaat
      421 taaggttatt tgtgtattta gacaataaaa aatccttcta actttgtcga ttagaaggaa
      481 atattttgtc atatgaaaaa tgaatttaag aaaaagtgta tgctataatt gatacgctgt
      541 cttcctgttt ggttgaaatc ttcagccaga aaggaggagt gttaaatgtt ttatgtgctt
      601 gatgtttgcg ttagaatcgt ggttaatgtt ttctcccatc tcattaacaa atggttaagg
      661 tgatatagct gattcataag cattttttat atactttttt cacataaaat act
//
LOCUS       DAGCWU010000310          710 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.323, whole genome shotgun sequence.
ACCESSION   DAGCWU010000310 DAGCWU010000000
VERSION     DAGCWU010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.323"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..699)
                     /locus_tag="MBZ71_002821"
     CDS             complement(<1..699)
                     /locus_tag="MBZ71_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4430692.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKK"
BASE COUNT          210 a          126 c           96 g          278 t
ORIGIN      
        1 tttcttaatt tcatgactaa attgtaaatc attccatatt gaccaagctt ttgtaacagt
       61 taccatttta ttatagtcaa tataagggta gtcaggttca tcaggcaaaa ttctttttgc
      121 aaagttagga taaaaatatt gtatgttaac ctcttttaca gtttctccag gttgcggtgc
      181 atgaatcatt acaccgttac ctttatatat acctacatga taagttgcac ctcgattgcc
      241 gtaaaataat aaatcaccag gcttaagtga atttaaactt acctctgtac cgtattgctc
      301 ttgattggtg gttcccatag gcagttcaat atttacagct tgtttgtaaa catatttcac
      361 taaaccacca cagtcaaaag aatttggtcc cgatgctccc cattcgtatg gctttccaat
      421 ttgctccatt gctttattaa tgactttggt ttgattagaa gtcatagtat aatttactat
      481 tcttgtttca ctattttcat ttgataaatt tgtaggtatt tccataatct gcttaaactg
      541 ttcttcacta taaccaactt ttaaagcttt ttctttttct atattatact gttcttctgt
      601 gtagtttatt tgttctgaaa aaacaatagt attgtagtac acactcaaaa cgattgagaa
      661 agacatcaca agaaataaag ttaatagttt tttcttcatt ttttacctct
//
LOCUS       DAGCWU010000311          705 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.324, whole genome shotgun sequence.
ACCESSION   DAGCWU010000311 DAGCWU010000000
VERSION     DAGCWU010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.324"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          260 a          109 c          139 g          197 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtc
//
LOCUS       DAGCWU010000312          696 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.325, whole genome shotgun sequence.
ACCESSION   DAGCWU010000312 DAGCWU010000000
VERSION     DAGCWU010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.325"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            2..208
                     /locus_tag="MBZ71_002822"
     CDS             2..208
                     /locus_tag="MBZ71_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430693.1"
                     /translation="MSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISI
                     IGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(299..664)
                     /locus_tag="MBZ71_002823"
     CDS             complement(299..664)
                     /locus_tag="MBZ71_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430694.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          263 a           95 c          122 g          216 t
ORIGIN      
        1 tttgtcttct aaaaaaaatt gggtatactc acaaactatt ttttatggag gaattgtttt
       61 aattagttta ctttcttcaa cactttattc tttgaatatt atagatgttt ccacctcaaa
      121 ttcaatatct attattggaa tcattattgc tgcaattatt acacaactgt ttttagtgtt
      181 tggagagaag aaaagaagta aaaaatagaa aaactagtga tagttttggt gatataagaa
      241 aaacatatat gtataaaaag aagaaagtag cttctctttg tacatatatg gtttgttttt
      301 atggatgccg agctttttta taagataagg ctaccgccca taacgaccca aggatttgtg
      361 gaactaagta aatacctgca agaagcaaga tcagctgggt cggttttaaa ggatgataga
      421 agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga agtgacagta gatataatat
      481 tggcaatgaa taaagatata atacgcctgt tcctttgaaa ataatatcca gcaaaaagag
      541 ataaaaatat caatgtgatg aacccgctta cggaatgata acgaaaatca agcagtaagg
      601 cgaaatgcac ataaggaaca aaagataaaa gcaacggaag gtttttccag caagttttta
      661 tcatctttca tcaccaccta acaagtgtta ttattt
//
LOCUS       DAGCWU010000313          690 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.326, whole genome shotgun sequence.
ACCESSION   DAGCWU010000313 DAGCWU010000000
VERSION     DAGCWU010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.326"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(44..502)
                     /locus_tag="MBZ71_002824"
     CDS             complement(44..502)
                     /locus_tag="MBZ71_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4430695.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
BASE COUNT          199 a          125 c           92 g          274 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcgtcaact
       61 ttctcttttc aaaaaaaatt tgatattttt ttagatttct ttttagtctt tgtaatactt
      121 tataagccac agaataactc acgtgatgct gtttagcaaa attttctact gagatatatt
      181 tttcctgaaa gaaagccaat accattttca tagataaaga atccgttata taacgagata
      241 acagagcttt gctagaacat tttccattaa tttccagaca tatatccaat tcactgacgg
      301 ttactcgcaa atttttctct agatcgaact tttgtaaatc atgtttcagc ttatcgatcg
      361 tttttttcaa gagaaatgtc gatagcgaca actcttcttg aacattcgat attgactctg
      421 ataaatttga gcttgcttct aaatatttaa gtaatgaata acttctgtga tcttctttgc
      481 ttaaaaaaaa ctcataattc attagtcatc agctctttct acgtatatta ctttccatgc
      541 attcaccaac tagctgggga taaaactgat ttcctggatc cgagtcactg ttgtcagcga
      601 ttcttgtttg tataatatac atcgtcctgt ctttttttta acattttgga gagcgtttta
      661 tttggccttt ctcgcgatca aacctgttat
//
LOCUS       DAGCWU010000314          690 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.327, whole genome shotgun sequence.
ACCESSION   DAGCWU010000314 DAGCWU010000000
VERSION     DAGCWU010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.327"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>690
                     /locus_tag="MBZ71_002825"
     CDS             <1..>690
                     /locus_tag="MBZ71_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HBT4430696.1"
                     /translation="IWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYT
                     IKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYG
                     LKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLAL
                     KGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRF
                     ESILLAYSVLL"
BASE COUNT          236 a          125 c          121 g          208 t
ORIGIN      
        1 ttatatgggg tataattaat ggctatacta gccaaagagc cacgtataga gcggtttgtt
       61 ccgtcttatt tcctaatggt gactttccta gtaggagtcg attcactcgc ttaagttcaa
      121 acttagctta cactatcaag attattcgat actttttcat caagaagctc accaaaggtg
      181 aactagtcgg aattatagat agttttccta gtccattatg taaaccagtt agaaatagac
      241 aagcaaaact attgaatcaa atagccaaag ttggctataa ctcaacaaaa aaatcttatt
      301 tttatggtct taaaattcat atgatcgtta ctaagactgg ctttccgatt acttactcaa
      361 tcacgaatcc aggtgtccat gacgtgaaag tgttagagac tttatcggaa gaagcgaatc
      421 ttcccaatat cctaggggac aaaggatata ttagccataa aatccatgaa aagcttgccc
      481 ttaagggcat tactatatcc gttccgccac gtaaaaacat ggataaatca gaaaaactag
      541 accacagctt acttggaaaa caaagaaaaa cagttgagac tgttttttct tccttagaaa
      601 aattaggttg tcaaaatttc aactcacgtt ctgttaaagg gctagagagt agatttgaaa
      661 gcatattact ggcttacagt gttctattaa
//
LOCUS       DAGCWU010000315          684 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.328, whole genome shotgun sequence.
ACCESSION   DAGCWU010000315 DAGCWU010000000
VERSION     DAGCWU010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.328"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..440
                     /locus_tag="MBZ71_002826"
     CDS             <1..440
                     /locus_tag="MBZ71_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBT4430697.1"
                     /translation="LGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSVIYFALLL
                     GIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFMKVADLDD
                     APGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            449..676
                     /locus_tag="MBZ71_002827"
     CDS             449..676
                     /locus_tag="MBZ71_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4430698.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          217 a          113 c          127 g          227 t
ORIGIN      
        1 ttttaggtat aagtgccgta tttattttgt tcggcgtact tttttatact cagatcgcta
       61 caagcgaaaa aggattcaat tttgattggc agaccacatt atttgtttct gtcatttact
      121 ttgcccttct cctagggatc gttattgctt atttcttatt gaaactattg aaaaaaatcg
      181 aagataacca agctttttcc actcaaagct taaacatact aaaaaaaatc gaacgaacca
      241 ttttgtttat tggtttcgcc ttttttggct tacttcctaa atttatgaaa gtagcggatt
      301 tagacgatgc tccaggtttg atgttggtag gaatcgctgt cgtattcatt ccttttgcag
      361 tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca tgcgatttta cttaagacag
      421 atcatgattt gacagtgtga ggtgcgacat ggcaattatc gttaatttag atgtagtatt
      481 agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa gtgggcatta cgatggcaaa
      541 catctctatt ctaaaaaatg gaaaagcaaa agccatccgt ttttccactc tcgaaaaaat
      601 ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc tataaaaatg actcgaatac
      661 tataccagaa gaataaaata aagt
//
LOCUS       DAGCWU010000316          680 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.329, whole genome shotgun sequence.
ACCESSION   DAGCWU010000316 DAGCWU010000000
VERSION     DAGCWU010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.329"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>680
                     /locus_tag="MBZ71_002828"
     CDS             <1..>680
                     /locus_tag="MBZ71_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HBT4430699.1"
                     /translation="IDAAIQFDFIYPIVESTYSTHGRPSIDPVVLIKLVFIQYLFGIR
                     SMRQTIKDVETNVAYRWFLGYSFEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQA
                     VKAGFVTEDNLYIDSTHIKANANKHKFTKEMTYGQAKAYQDELEDEINKERIAVGKRP
                     FTWDTESELSLQKISHSDPESGYYVKGEREKQFAYSAHTSCEDNGFILSTLITPGNVH
                     DSQVAIDLV"
BASE COUNT          225 a          117 c          121 g          217 t
ORIGIN      
        1 attgatgctg cgattcagtt tgatttcatt tatcccattg tagaatctac gtattcgacc
       61 catggtcgtc ccagtattga tccagtggtc ttaattaaat tagtatttat ccaatattta
      121 tttggtatcc gttcgatgag acagactatt aaagacgttg aaacaaatgt tgcttatcgt
      181 tggtttttag gttattcatt cgaggatcct atcccgcatt tctctacttt tggtaaaaac
      241 tatgttcgaa gattcagaga aacaacttta tttgaagaca ttttctctca tattctagaa
      301 caagctgtaa aagctggctt cgttacggaa gacaaccttt atattgattc tactcatatt
      361 aaggcaaatg ccaataaaca taagttcaca aaagaaatga cctacggtca agctaaagct
      421 tatcaagatg aattagaaga tgaaataaat aaagaacgta ttgctgtagg aaaacgtcct
      481 tttacatggg acacagaaag tgagttgagt cttcaaaaaa taagtcattc agatccagaa
      541 agtggttact atgttaaagg tgaacgagaa aaacaatttg cttattccgc gcatacttcc
      601 tgtgaagata atggttttat cttatctaca ttaatcacac caggaaatgt acatgacagc
      661 caagtcgcaa tcgatctggt
//
LOCUS       DAGCWU010000317          678 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.330, whole genome shotgun sequence.
ACCESSION   DAGCWU010000317 DAGCWU010000000
VERSION     DAGCWU010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.330"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          274 a           77 c           81 g          246 t
ORIGIN      
        1 aacaaaatag cgaggttaag ctatttatta gttcatatat tatattcaga ttaatatgat
       61 ccagtattga tctgaaggca aaaccaatat atttctacgg attcttgtaa aactatttaa
      121 attttcctgc taataaaata acaaaaatag aatatttagc ttttttatac cattttacaa
      181 taacatgatt ttatttgtaa tattaaaaca tttaaatttt gatttttttt caaattagat
      241 caattaatat ccttataaaa gagcataata aatgattttt acatcgttat ttattgatat
      301 taatctcatc caaaaaaatg aaagcgcata ctttatgggt gattaaatgt gattttaata
      361 catataccaa ttaaaagaaa acttttttag atttttactt acaaatttat aaaaaaacaa
      421 gataattgat actttgattt tgcgaacatt ttatctttcc gcctaattca gctgcaatat
      481 catgattata tcagtaacaa atcgcttttt tagctaattt aagcatcaaa taattattct
      541 taatcagtga aaaaaaataa cgaataaatg gattttggga ataggtaaaa acttgatcgg
      601 tttctggata aaaataacat gaaaaaggaa atataaaata acatggagcg tgggaggaat
      661 aaagaaattt tacactcg
//
LOCUS       DAGCWU010000318          649 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.331, whole genome shotgun sequence.
ACCESSION   DAGCWU010000318 DAGCWU010000000
VERSION     DAGCWU010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.331"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            119..649
                     /locus_tag="MBZ71_002829"
                     /pseudo
     CDS             119..649
                     /locus_tag="MBZ71_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          252 a           96 c          107 g          194 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaaattagc tttgagccaa ctgattcatt tcagtatctt agcaaagata ttgaaattga
      181 agatatttat aaaattttag ctcaagagct atctaaaaat gttattagtt tgccaaaggt
      241 tttttatttt ccagccgaaa gaactggtat aaatgtcttt aagaacgaac taaatgaaag
      301 tcgcttaaaa acatatgata ccttgctgaa tactgtgcaa tttactaatt taactaacca
      361 aaaaaagaaa gctgccctca agtacgatct gttaaaaaag aatattgagc tgttaatgga
      421 gacaacggat aatagttctt atccaaagcc aatatcggat tatattaatt atctgaataa
      481 catgaaaaat agatatgaac agaaaaatgt caataatata gcatcatata ttagagatga
      541 gattattatg ggtaaatatg aaattgacga aaaagataat tctgtaagtt ttagacaaaa
      601 gtttggtaaa aatagatata gaaaagaacg aattcctttt cacgttgtg
//
LOCUS       DAGCWU010000319          643 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.332, whole genome shotgun sequence.
ACCESSION   DAGCWU010000319 DAGCWU010000000
VERSION     DAGCWU010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.332"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..277
                     /locus_tag="MBZ71_002830"
     CDS             <1..277
                     /locus_tag="MBZ71_002830"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBT4430700.1"
                     /translation="YEKVQIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDK
                     IILMAYCELNENEVKDHRPKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            479..>643
                     /locus_tag="MBZ71_002831"
     CDS             479..>643
                     /locus_tag="MBZ71_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4430701.1"
                     /translation="MSRRQRRTYSKEFKQQIVNLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
BASE COUNT          251 a           80 c          127 g          185 t
ORIGIN      
        1 atatgaaaaa gtacaaatag ttgatgttaa taatggaaat agatttgaaa cttatacaat
       61 agctggagag agaggaacag ggatgatttg cttgaatgga gctgcagcta gatgtgtatc
      121 aactggagat aaaataattt taatggcata ttgtgaatta aatgaaaatg aggttaagga
      181 tcatagacct aaggttgttt ttgtcgatga taataataaa gttgaaagag taacatcata
      241 tgaaaaacat ggaaggttat cagatataat tctttaggtt ttgtaatgtt atttatatat
      301 agctagacta ttattgaaat atttattgat tactaattgg atagatgtca ataaagtaga
      361 cacaaaaaga gaagatacag actgtagaca aagtccattt tatgtgaatc ggctacacta
      421 gactagacag aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat
      481 gtcaagacgt caacgaagaa cctattcaaa agaattcaaa caacaaatcg tcaatctcta
      541 tctcgctggt aagcctcgcg cagaaattat tcgagagtat gagcttacgc cttcttcttt
      601 cgataaatgg atgaagcaag cacaatcaac gggctcattc aaa
//
LOCUS       DAGCWU010000320          627 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.333, whole genome shotgun sequence.
ACCESSION   DAGCWU010000320 DAGCWU010000000
VERSION     DAGCWU010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.333"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            330..>627
                     /locus_tag="MBZ71_002832"
     CDS             330..>627
                     /locus_tag="MBZ71_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4430702.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFP"
BASE COUNT          230 a           69 c          104 g          224 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaa
//
LOCUS       DAGCWU010000321          619 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.334, whole genome shotgun sequence.
ACCESSION   DAGCWU010000321 DAGCWU010000000
VERSION     DAGCWU010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.334"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            9..>619
                     /locus_tag="MBZ71_002833"
     CDS             9..>619
                     /locus_tag="MBZ71_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4430703.1"
                     /translation="MIREGISEYLSEFGYTVIQAKDGREALSKFNSDINLVILDIQIP
                     FINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFSLPVLKARIDS
                     LIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLDNNGQVLTRMQ
                     IIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYK"
BASE COUNT          259 a           53 c          112 g          195 t
ORIGIN      
        1 atgataatat gataagagag ggaataagtg aatatctttc agaatttgga tatactgtta
       61 ttcaagctaa agatggaaga gaagccttgt caaaatttaa tagcgatata aatttggtta
      121 tcttagacat tcagatacct tttataaatg gtttagaagt gttgaaagaa attagaaaga
      181 aaagcaattt accaattcta atcttgactg catttagtga tgaagaatat aaaattgatg
      241 catttactaa tttagtagac ggatatgtag aaaagccatt ttcattgccg gtcttaaaag
      301 ctagaataga ttctttaatt aagaagaatt atggacattt agagaagttt gaatataagg
      361 atctatcggt taattttaat agctatacag ctaaaataaa tggtgaagaa atagatgtaa
      421 atgcaaaaga actagaagta ttaaaatgtt tattggataa taatggacaa gtgttaacaa
      481 gaatgcaaat tattgattat gtatggaaag atagcgaaga aactccttat gatcgagtaa
      541 tagatgtata tataaaagaa cttagaaaaa aattacaatt agactgtata actactataa
      601 gaaatgtagg atataaatt
//
LOCUS       DAGCWU010000322          616 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.335, whole genome shotgun sequence.
ACCESSION   DAGCWU010000322 DAGCWU010000000
VERSION     DAGCWU010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.335"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          208 a          108 c           80 g          220 t
ORIGIN      
        1 ctaggaaata tggtatttgg tatcagtaaa gaattcagtt ggactactac attatgggaa
       61 tctctattct ttttcatctt cgtatttttg ttacaaaatt atcgaaaaaa ataaacatgt
      121 gccatctatc tgttggcaca tgttttagga tttatggtac tttttattcg gcaatccatt
      181 ttatttttct acttcttctt aatacctcta acacttttga tttataaact aatccaatgc
      241 atctacttgc gcttattaag ctatttcagc tagatagtac caccccatgc gtcaatgctt
      301 gattttcctt ttgtataaca ttgtataagc aataaaggac aaataagtgg tgattacacc
      361 aaaccattga ataagaaaaa ttctgatacg taaataaaca tgaacttgaa aaaattaatg
      421 aatcggttag ctactacgta gttgtctttt ttctcataca gttctgttaa cttacacttc
      481 tcttcgctag ataataaaaa atcctccaac tgtattgtcc aatataatcc caggcatcta
      541 aactagcatt atagaagaga cacacataga atctaaaata actggtttta aatgtttctt
      601 acaatcaaaa aaagct
//
LOCUS       DAGCWU010000323          594 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.336, whole genome shotgun sequence.
ACCESSION   DAGCWU010000323 DAGCWU010000000
VERSION     DAGCWU010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.336"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..156
                     /locus_tag="MBZ71_002834"
     CDS             <1..156
                     /locus_tag="MBZ71_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBT4430704.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>594
                     /locus_tag="MBZ71_002835"
     CDS             175..>594
                     /locus_tag="MBZ71_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4430705.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKE"
BASE COUNT          261 a           46 c          104 g          183 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaa
//
LOCUS       DAGCWU010000324          590 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.337, whole genome shotgun sequence.
ACCESSION   DAGCWU010000324 DAGCWU010000000
VERSION     DAGCWU010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.337"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>590
                     /locus_tag="MBZ71_002836"
     CDS             <1..>590
                     /locus_tag="MBZ71_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4430706.1"
                     /translation="KEKVLSGVNQKFELGKFYAIVGKSGTGKSTLLSLLAGLDKPQTG
                     KILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIRLVNKSADESILFELGL
                     DKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLA
                     KDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNK"
BASE COUNT          253 a           62 c          104 g          171 t
ORIGIN      
        1 aaagaaaaag ttttgtcagg agtaaatcaa aaatttgaac ttgggaagtt ttatgcgata
       61 gtaggaaagt caggaacagg aaaatctaca cttctttcct tacttgctgg acttgataaa
      121 cctcaaacag gaaaaatatt gtttaagaat gaagatatac aaaataaagg atatagtaat
      181 catagaaaaa ataatatatc tttagtattt caaaactata atttaataga ttatttatca
      241 ccaattgaaa atattagatt agtaaataaa tcagcagatg aaagtatctt gtttgaacta
      301 ggtttagata aaaaacaaat aaaaagaaat gttatgaaat tatctggtgg acagcaacaa
      361 agagtagcta ttgctagagc attggtatca gatgctccaa taatactagc tgatgagcct
      421 actggtaatc tagacagtgt tactgctgga gaaataatta atatattaaa gacattagct
      481 aaagacagaa ataaatgtgt aatagttgta acacatagta aggaagtagc agattctgcg
      541 gatatcattt tagaactaag tggtaaaaag ttgaaaaaag taaataaaat
//
LOCUS       DAGCWU010000325          575 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.338, whole genome shotgun sequence.
ACCESSION   DAGCWU010000325 DAGCWU010000000
VERSION     DAGCWU010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.338"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            312..>575
                     /locus_tag="MBZ71_002837"
     CDS             312..>575
                     /locus_tag="MBZ71_002837"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBT4430707.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          195 a          105 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DAGCWU010000326          545 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.339, whole genome shotgun sequence.
ACCESSION   DAGCWU010000326 DAGCWU010000000
VERSION     DAGCWU010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.339"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            130..417
                     /locus_tag="MBZ71_002838"
     CDS             130..417
                     /locus_tag="MBZ71_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430708.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          196 a           86 c           99 g          164 t
ORIGIN      
        1 tttttttgtg aaaagtacaa ttagttgtct ttcttcgaat tttttgtcac aataagcagc
       61 aaagataaag ctgttacata aaagaacaag cagctttatt tgattttgaa aagaaaggtg
      121 agaaagaaga tgaataaaga atattttgtc caacaagatg catttttaga agattataaa
      181 agtgcttcga agaaattaac gatcaatatt gatgagttgg cagatcaagt actctctcag
      241 ttgaatgaaa cgcaagagga atattacacg atgccagcaa atgacgccaa agataatgtt
      301 tctcatcgtt ttcctttcag tagacgcatc tactcagatg aaaatgacgg cacattgaat
      361 acagagtaca catatgaagg tcagccatat gtgctagaaa ctgatgatga tctttaaaga
      421 cagtttttga aaaggccgct catccttaag tgatgacaaa aaatcatcac aattaccgat
      481 gactgtctct ttactatctt ttaatcagaa aaaggggtta tgacaagctt tgtcaacccc
      541 ttttt
//
LOCUS       DAGCWU010000327          544 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.340, whole genome shotgun sequence.
ACCESSION   DAGCWU010000327 DAGCWU010000000
VERSION     DAGCWU010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.340"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..275
                     /locus_tag="MBZ71_002839"
     CDS             <1..275
                     /locus_tag="MBZ71_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430709.1"
                     /translation="IKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLEKPLQHE
                     NNDINFRFVYESKSKTWLVDQLSTINTSFHELPKPENEVEMVIKIF"
BASE COUNT          185 a           97 c           68 g          194 t
ORIGIN      
        1 acattaaaaa aataaaagct tacgaccatc cgttaaatct gacactaaat aatggactgc
       61 tttcttttgt attagcaggt tacgttgact atgaatattc accagattat ttggagaaac
      121 ctcttcaaca cgagaacaat gatattaatt ttagatttgt ctacgagtca aaaagtaaaa
      181 catggttagt tgatcaatta agcacgataa acacaagctt ccatgaatta cctaaacctg
      241 aaaatgaagt tgagatggtc attaaaatct tctaaaaaag aagcgttgaa ggggaaatat
      301 tcctttcaac gctctttttt atatgcttag aaacataaat aaattgtata ctttcttttt
      361 agcccctatt acttttcacg aaaaatatct ttttttcgac gctagcgttt ctctacttct
      421 aatactgtac acagtttttg tattattgaa aaattactca ttatttagca ttaatccaca
      481 tcgttttatc aatcgacctt catactaaac tactttaaag tcatcttcct tctcgttttt
      541 catt
//
LOCUS       DAGCWU010000328          540 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.341, whole genome shotgun sequence.
ACCESSION   DAGCWU010000328 DAGCWU010000000
VERSION     DAGCWU010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.341"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(121..204)
                     /locus_tag="MBZ71_002840"
     tRNA            complement(121..204)
                     /locus_tag="MBZ71_002840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Leu,seq:cag)
BASE COUNT          129 a          112 c           76 g          223 t
ORIGIN      
        1 tttttgattt atttgaatct ggttccgttg tttaaaattt ttcctatatt cttttagata
       61 atatgttatt ttctttctca tcgctccagg tgtaccttct ctttataaaa aaacaaatta
      121 tgcggctaag aagactcgaa cttccacgag gtcaccctca ctagcgcctg aagctagcgc
      181 gtctgccaat tccgccatag ccgcatcaca tcttttagtt taggattaca tgtgtttttt
      241 gtcaagaaca tatttttgtt ctttttcatt tctgtacgcc ctcccttatg ccatctacac
      301 aaataattca tccgagcaac tccatcatct attgagggtt ggtgaaaaaa tctgttttat
      361 ccatatgatc gatttcaatg ttaaacgaac gactctggga ttagtaacta ttttattttt
      421 ttcttcattt cttttttcct ctttttctcg attcagctat cttctttcat ttggatattg
      481 attatttctt ctgttgatta ctatccttct ggaattctag acgctgaagt aattcacgtt
//
LOCUS       DAGCWU010000329          528 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.342, whole genome shotgun sequence.
ACCESSION   DAGCWU010000329 DAGCWU010000000
VERSION     DAGCWU010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.342"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          220 a           71 c           56 g          181 t
ORIGIN      
        1 ttactttata taaataaata taaaaaaatg gtgctgcaat ggaacgtatc tgattcgtca
       61 taatcacgta catctcatta ttttttctag acaatctctc actaaaggtt gttggactat
      121 aatgaatgaa tatgttaaca atatctgtaa aactatttat agaacgaccg ctataaatca
      181 attgatataa aaaagtgaat aagtatatta tagtaaatac aatcaaaatg ctactattta
      241 atattatatc tgaatcactt aatgcttttc ctttttttaa ccgttcacta aaccaaatca
      301 taaatacaaa tacaatcata aataacacaa attgcattag atatatgtat ggagtagata
      361 ttttattttg ctgaaattga ataccgatac ctgaatacaa agtgaatcct gttaacatta
      421 aatacgcaaa tattttatgg tagtaatcaa atatacatct acgtataaac aaaaaagtta
      481 gcatatcagc aaaaataata attgttaaaa tcataatttt atcaatat
//
LOCUS       DAGCWU010000330          514 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.344, whole genome shotgun sequence.
ACCESSION   DAGCWU010000330 DAGCWU010000000
VERSION     DAGCWU010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.344"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..132)
                     /locus_tag="MBZ71_002841"
     CDS             complement(<1..132)
                     /locus_tag="MBZ71_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBT4430710.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAGCWU010000331          496 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.345, whole genome shotgun sequence.
ACCESSION   DAGCWU010000331 DAGCWU010000000
VERSION     DAGCWU010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.345"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(73..270)
                     /locus_tag="MBZ71_002842"
     CDS             complement(73..270)
                     /locus_tag="MBZ71_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4430711.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          161 a           90 c           72 g          173 t
ORIGIN      
        1 attacggtga ggaatttttt ctaccgacat aaacaaaaaa accacctcat atgaggcggt
       61 taacgttcaa cattatttta cgatatttac tgcttgagga ccacgttgtc cttcttcaat
      121 atcataagta actgcttggc cttcatctaa agacttaaag ccttctcctt ggattgctga
      181 gaaatgagcg aatacgtcat ttccatcttc accagtgatg aaaccaaaac ctttatctga
      241 gttaaaccat tttactgtac cgttattcat atatatttcc tcctgatacg tatataatac
      301 gtgttttatt gcaattaata ttacttggta aaaggagttt ttatagtgtg aatatatcgc
      361 tcagattacg tttcaaatta gattacttat ttactataac acggatgtaa tgctaacaca
      421 aggataatca ctcgatatac ggtttaaata ttattatttg tcaaattaag cgagacaaaa
      481 ctttccattt acgtaa
//
LOCUS       DAGCWU010000332          493 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.346, whole genome shotgun sequence.
ACCESSION   DAGCWU010000332 DAGCWU010000000
VERSION     DAGCWU010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.346"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..209)
                     /locus_tag="MBZ71_002843"
     CDS             complement(<1..209)
                     /locus_tag="MBZ71_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4430712.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(308..>493)
                     /locus_tag="MBZ71_002844"
     CDS             complement(308..>493)
                     /locus_tag="MBZ71_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430713.1"
                     /translation="FELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQE
                     VFREFRNKIIGFSQTTK"
BASE COUNT          151 a           91 c           58 g          193 t
ORIGIN      
        1 aaatcagata cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac
       61 tcccattttc tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta
      121 gtaatttcta tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct
      181 ttgaagtttg ttggagtaat agtatccaaa atgaatacct ctcttctcta tacctctatt
      241 atgaagtgcc cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat
      301 taagctttca ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa
      361 cttcttgtgg ttgataatag tctggatcta tttcaaatgt ttgtgagaat aattcattga
      421 atttttctac tgaaataatg attttaccat tcttctctcg actgttttct attatttctg
      481 cttctaattc aaa
//
LOCUS       DAGCWU010000333          472 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.347, whole genome shotgun sequence.
ACCESSION   DAGCWU010000333 DAGCWU010000000
VERSION     DAGCWU010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.347"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            194..412
                     /locus_tag="MBZ71_002845"
                     /pseudo
     CDS             194..412
                     /locus_tag="MBZ71_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          162 a           66 c           76 g          168 t
ORIGIN      
        1 atttttctaa caaaagcaca tattttaata tatactacct attttaggta gtatatattt
       61 ttagatacaa tatcgatcag ttttgaggaa atgaaattct agatattttt tgctacatag
      121 agttactttc aatatttttt aacgtaatta ggcagaagtc actcatcctc tataggtttg
      181 tgatgaaatt gccattgatc catttaagta agagcaaaaa actggaggtt tatcaggtaa
      241 ttggtctaat ggatttaagt atgcggtcac cgtgtataga gttgcttatg agattcaaga
      301 aaataaagtt attccagtat tgctatatgg aacacataac aatttttatg aattagccga
      361 gaacactcgc aactttattc gtgtagatga atcggcttcg gttgagttat aactttagct
      421 atatcaatca accgaccgac atatgttaaa tctatatata ataaaaatac ct
//
LOCUS       DAGCWU010000334          465 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.348, whole genome shotgun sequence.
ACCESSION   DAGCWU010000334 DAGCWU010000000
VERSION     DAGCWU010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.348"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(13..86)
                     /locus_tag="MBZ71_002846"
     tRNA            complement(13..86)
                     /locus_tag="MBZ71_002846"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Ile,seq:cat)
BASE COUNT          156 a           74 c           93 g          142 t
ORIGIN      
        1 tggctacatt aatgtaccct gtaggactcg aacctacgac cggacggtta tgagccgtct
       61 gctctgacca actgagctaa gggtagtggt tgttgccaca taaagccata aacaatcaac
      121 cagtagaatg cgtggcaaca aacctgttat cgcatatctt ggagtgtgac tatttatgag
      181 tgatagtgaa gatatgcgtc tccatatatg tatggttgag acagataaac atgtatgaaa
      241 aacgttgtta aatcaacgtt tttttatttt ggtcaaggag agaaaagtag agaaatacaa
      301 aattttttgc attgattttg cattcaaaaa aacattcaaa catttagaaa tccccaatac
      361 gtattcagga aaatctattt taaggtttac aaaaagaact tttgttcgta cactcttttt
      421 gaggtgaact tttatgctga tggaaggaaa aacacaatta tggtt
//
LOCUS       DAGCWU010000335          436 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.349, whole genome shotgun sequence.
ACCESSION   DAGCWU010000335 DAGCWU010000000
VERSION     DAGCWU010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.349"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>436
                     /locus_tag="MBZ71_002847"
     CDS             <1..>436
                     /locus_tag="MBZ71_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4430714.1"
                     /translation="KLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSD
                     FSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFV
                     EKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILIL"
BASE COUNT          172 a           36 c           79 g          149 t
ORIGIN      
        1 taaaattagg tgatgaagtt gatcttgaat tactagatat tgaaaaaagt ggaaaaataa
       61 aaagtcataa atttaaaatt atagggatct tttctggtaa aaaacaggaa acatatacag
      121 gattatcttc tgattttagt gaaaatatgg tgtttgtaga ttattcaaca agccaagaga
      181 tattaaataa ttcagaaaat aatgaaattg caaataaaat tttaatgtat tctggtagtg
      241 cagaatctac agacttagcc ttaaacaaat tgaaagagct taaaattgat gagtcaaagt
      301 attttgttga aaaagattct aatgcgtttg aagagtcttt agagtcagtg agtggaataa
      361 aatatataat aaaagtaatg acttattcta ttatgttagg tgggatggtt gttctttcat
      421 taatcttgat tctatg
//
LOCUS       DAGCWU010000336          429 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.350, whole genome shotgun sequence.
ACCESSION   DAGCWU010000336 DAGCWU010000000
VERSION     DAGCWU010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.350"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          173 a           63 c           75 g          118 t
ORIGIN      
        1 gaattgtcca tcactgttat tcaaatcatt ttatcaaaac tacatagtaa atgttaaaaa
       61 gcaacatgac ataatatata gaaggcttta cagtatatat gggagcttac taaaaaagaa
      121 tggagtaaga gttaacacaa tgggccatta gctgaagttt taagcacttc aaggtatgaa
      181 acagaatggc ctgtaatact acatgattat ttagactgct caaactgcta tcagtctggt
      241 ggaatgaata aagtattata ctaaaagcag atccaactac aacgtaacag gaaaccagtt
      301 gtatcaggaa aagtgatagt acatggagaa gaaaaggccc cctttgtatg agtcagtaca
      361 tatcttattt atagataaaa cggtattcaa tcgaaataaa aaatctgaat aatagacgta
      421 aataacttc
//
LOCUS       DAGCWU010000337          400 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.351, whole genome shotgun sequence.
ACCESSION   DAGCWU010000337 DAGCWU010000000
VERSION     DAGCWU010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.351"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..382
                     /locus_tag="MBZ71_002848"
     CDS             <1..382
                     /locus_tag="MBZ71_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4430715.1"
                     /translation="KEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIEN
                     QDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYN
                     QKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          154 a           71 c           62 g          113 t
ORIGIN      
        1 aaaagaaatc tgtgatgact ttaaggctgt ttatcaagct aactcaaaag aagaagcgaa
       61 taccttctta tccggcatga ttgagaaatg gaagaaaaac tatcctaaag tgacgcagtc
      121 actcatagaa aaccaagact tattaacttt ttatgatttt ccacctagca ttcgtagaac
      181 catttactca accaatctaa tcgagtcttt caataagcaa attaaaagat acagccgtag
      241 aaaagagcag tttcaaaatg aagaatcact agaacgcttt ctagtcagca tttttgatac
      301 atacaatcaa aaatttctaa acagaagcca taaaggtttt caacaggtaa ccgatacatt
      361 agtttcaatg tttactgagt aactaattat tttgcaggag
//
LOCUS       DAGCWU010000338          399 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.353, whole genome shotgun sequence.
ACCESSION   DAGCWU010000338 DAGCWU010000000
VERSION     DAGCWU010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.353"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>399
                     /locus_tag="MBZ71_002849"
     CDS             <1..>399
                     /locus_tag="MBZ71_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4430716.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALMASMLEMYVSGVST
                     RKVSTIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQS"
BASE COUNT          137 a           75 c           78 g          109 t
ORIGIN      
        1 taggcacgct agaattaaaa gtacccagaa cacgtgatgg ccatttttca cccacagtgt
       61 ttgaacgtta tcaacgaaac gaaaaagccc tcatggcttc aatgttggaa atgtatgtat
      121 caggcgtttc aactcgtaaa gtatcaacaa ttgtggaaga actttgtggt aaatccgtct
      181 ctaagtcctt cgtttctagc ttaacagaac agctagaacc tatggttaac gagtggcaga
      241 atcgtttatt atcagaaaaa aattatcctt acttaatgac cgatgtactc tatataaaag
      301 tacgagaaga aaatcgagta ctctcaaaaa gctgtcatat agcgattgga ataaccaaag
      361 atggcgaccg tgaaattatc ggcttcatga ttcaaagtg
//
LOCUS       DAGCWU010000339          393 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.354, whole genome shotgun sequence.
ACCESSION   DAGCWU010000339 DAGCWU010000000
VERSION     DAGCWU010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.354"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          144 a           69 c           47 g          133 t
ORIGIN      
        1 gttagcattc ttttttgtaa acctatactc ctcagtaaaa cttttagtag ccataacaat
       61 cgcctccgtt tatgtaccaa cacaaaaata tataggaaaa aacacgtcaa aataacgtac
      121 ttgtacaaaa caatcataca tcattttacg ctttaacttt cctttgttgt aagttcaata
      181 taccatcatt ggtttaattg tacaagagaa acggctaaaa aatcagctaa ttttgttagc
      241 aactttgtta gcatatctga cagtagatca atagtaaaag tgcctgtata taaggaaaaa
      301 ctgtattttt actatctaaa ttcgtaaaca tttttgctta caaattagct aataatatta
      361 gcaactatat tgaaaactat atgtactgcc cct
//
LOCUS       DAGCWU010000340          370 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.355, whole genome shotgun sequence.
ACCESSION   DAGCWU010000340 DAGCWU010000000
VERSION     DAGCWU010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.355"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..220
                     /locus_tag="MBZ71_002850"
     CDS             <1..220
                     /locus_tag="MBZ71_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBT4430717.1"
                     /translation="DYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSE
                     EEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT          142 a           71 c           63 g           94 t
ORIGIN      
        1 agattacttt actcaggcag acttgatcat tacactttgt ggtgatgcca aagacaagtg
       61 ccctgccgtt ccaaacaatc acgaacacct tcactgggac ctaaaagatc ctgcacaagc
      121 tgtcgggagc gaagaagaaa tattaacgga attcagaaaa gtcagagaag gaatcaaaag
      181 aaaagtttta gacttaaatc aacgtacatc gatcgtataa agaagtattc aaaatgattt
      241 ttcaagcact aagaaacaat gttaaaaaaa tagctttcca tattttacaa aattgtgcct
      301 tattgccgac aaattgtctc ttgaacaccg tttaccagta gtcaagacat cgtaagcaac
      361 tataaaaaat
//
LOCUS       DAGCWU010000341          367 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.356, whole genome shotgun sequence.
ACCESSION   DAGCWU010000341 DAGCWU010000000
VERSION     DAGCWU010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.356"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>367
                     /locus_tag="MBZ71_002851"
     CDS             <1..>367
                     /locus_tag="MBZ71_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430718.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPN"
BASE COUNT          111 a           71 c           72 g          113 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaata
//
LOCUS       DAGCWU010000342          349 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.357, whole genome shotgun sequence.
ACCESSION   DAGCWU010000342 DAGCWU010000000
VERSION     DAGCWU010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.357"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            72..347
                     /locus_tag="MBZ71_002852"
     CDS             72..347
                     /locus_tag="MBZ71_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430719.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          155 a           53 c           51 g           90 t
ORIGIN      
        1 atccttcttg ttttcatata accaaaggcg ctatgatttc catatgaaaa aaataaagtc
       61 aggtgaaaaa aatgaaactt gaggataaaa aagtcatttt gaagaaaatc aaacgactac
      121 cagaccaaat agatggctat acgatctgtt ctttcaaaaa agacaggagt ataaaagctt
      181 atacgcaaaa cacagaaaaa ggaataatct atttcctaca tgaagaaggt tacgataccc
      241 aaacctttat gtttactgaa aaaaaagaat ttcataaaca gatgaaaaaa ctgattgaaa
      301 aagaatttcc aagaagtcat aaactctatg tgaatcagca attttaact
//
LOCUS       DAGCWU010000343          344 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.358, whole genome shotgun sequence.
ACCESSION   DAGCWU010000343 DAGCWU010000000
VERSION     DAGCWU010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.358"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            109..312
                     /locus_tag="MBZ71_002853"
     CDS             109..312
                     /locus_tag="MBZ71_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBT4430720.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT          147 a           53 c           72 g           72 t
ORIGIN      
        1 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
       61 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
      121 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
      181 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
      241 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
      301 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaat
//
LOCUS       DAGCWU010000344          333 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.359, whole genome shotgun sequence.
ACCESSION   DAGCWU010000344 DAGCWU010000000
VERSION     DAGCWU010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.359"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>333)
                     /locus_tag="MBZ71_002854"
     CDS             complement(<1..>333)
                     /locus_tag="MBZ71_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4430721.1"
                     /translation="ELAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLL
                     RQDPVFKAVLDRKELASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELII
                     DLDSTHSDT"
BASE COUNT          101 a           56 c           72 g          104 t
ORIGIN      
        1 tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc gtatcattac ggatgaggcg
       61 tgctttatcg ataagttctt ggttcaaagc ttgaagttca tggatattct cctcagatag
      121 tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt tctttcctat ctaaaacagc
      181 tttaaacaca ggatcttgtc tcaggagatt agctgcagaa tcagctgagt aaccagcaat
      241 taattgcata atgaactgct caagtatgga taagttgtca tgagtaaaat acgctcgttc
      301 gtcttcaatg tgaatgtgtt gcttagccag ttc
//
LOCUS       DAGCWU010000345          332 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.360, whole genome shotgun sequence.
ACCESSION   DAGCWU010000345 DAGCWU010000000
VERSION     DAGCWU010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.360"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>332
                     /locus_tag="MBZ71_002855"
     CDS             <1..>332
                     /locus_tag="MBZ71_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430722.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAGCWU010000346          330 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.361, whole genome shotgun sequence.
ACCESSION   DAGCWU010000346 DAGCWU010000000
VERSION     DAGCWU010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.361"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(57..173)
                     /locus_tag="MBZ71_002856"
     CDS             complement(57..173)
                     /locus_tag="MBZ71_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBT4430723.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT          123 a           66 c           41 g          100 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggtg caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gatacttaaa taatagcacg
//
LOCUS       DAGCWU010000347          326 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.362, whole genome shotgun sequence.
ACCESSION   DAGCWU010000347 DAGCWU010000000
VERSION     DAGCWU010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.362"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          118 a           34 c           62 g          112 t
ORIGIN      
        1 ggaaatataa gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa
       61 gaactgatct tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg
      121 ccgactatga gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg
      181 ggtgcttcga tagaacatta taagacatgg agttcaagaa ttattataga actattctta
      241 atgttctttt ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga
      301 attgtagtat tactttgcaa gtatgt
//
LOCUS       DAGCWU010000348          323 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.363, whole genome shotgun sequence.
ACCESSION   DAGCWU010000348 DAGCWU010000000
VERSION     DAGCWU010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.363"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="MBZ71_002857"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="MBZ71_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBT4430724.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAGCWU010000349          322 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.364, whole genome shotgun sequence.
ACCESSION   DAGCWU010000349 DAGCWU010000000
VERSION     DAGCWU010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.364"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(44..>322)
                     /locus_tag="MBZ71_002858"
     CDS             complement(44..>322)
                     /locus_tag="MBZ71_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4430725.1"
                     /translation="GITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           87 a           81 c           60 g           94 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt cc
//
LOCUS       DAGCWU010000350          321 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.365, whole genome shotgun sequence.
ACCESSION   DAGCWU010000350 DAGCWU010000000
VERSION     DAGCWU010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.365"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(69..321)
                     /locus_tag="MBZ71_002859"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="MBZ71_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAGCWU010000351          319 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.366, whole genome shotgun sequence.
ACCESSION   DAGCWU010000351 DAGCWU010000000
VERSION     DAGCWU010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.366"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            66..>319
                     /locus_tag="MBZ71_002860"
     CDS             66..>319
                     /locus_tag="MBZ71_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBT4430726.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYR"
BASE COUNT          112 a           52 c           56 g           99 t
ORIGIN      
        1 taaaacttgt ccattatttc aaaatttgtt atagtctttt tattcaaagc gtaaaaggag
       61 atcctatgtt aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc tatcttatga
      121 aagaccgtga tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa aatagagtaa
      181 gcagctattt tcatcagaat aaacagcatt caaaaaaagt attacgtatg attcatcata
      241 taacagattt tgatttcgtc gtagtagaca cggaattaga tgctttattg ctcgagtgtc
      301 aattgatcca acattaccg
//
LOCUS       DAGCWU010000352          308 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.368, whole genome shotgun sequence.
ACCESSION   DAGCWU010000352 DAGCWU010000000
VERSION     DAGCWU010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.368"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>308
                     /locus_tag="MBZ71_002861"
     CDS             <1..>308
                     /locus_tag="MBZ71_002861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430727.1"
                     /translation="EWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANTP
                     EEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIV
                     "
BASE COUNT          128 a           35 c           52 g           93 t
ORIGIN      
        1 agaatggaat aatctgactc aaaataataa aaataattct ggatgtggaa ttatctttat
       61 gtttgttata gttttgggaa tagtagtact tttaataact gtcaagatag tagattactt
      121 tgcgaatact cctgaagaac aaatacagag aaaacttcct gagtttgaaa gagtaccacc
      181 ttatgatcca agagataatt catttcaagt aaaaaaatta aaagagtctc caaatgaaat
      241 gtggtttgaa gttacagttg aatcaagcga cttcaaagaa aaaaagaaag aatacaatta
      301 tattgtaa
//
LOCUS       DAGCWU010000353          285 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.denovo.370, whole genome shotgun sequence.
ACCESSION   DAGCWU010000353 DAGCWU010000000
VERSION     DAGCWU010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.denovo.370"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            41..>285
                     /locus_tag="MBZ71_002862"
     CDS             41..>285
                     /locus_tag="MBZ71_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430728.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVK"
BASE COUNT          134 a           39 c           46 g           66 t
ORIGIN      
        1 aacaattaat aggaacgtat attcgaaagg aatagaaata atgtggttag aaaaaataaa
       61 tgacaaaaaa tttaagtaca ccgaacgtta tacggatccc ttaactgaaa aaaagagaaa
      121 agtgtccgtt actttatcta gcaattcacg ccaagcttgg aatcaagcta acttactttt
      181 gaatgaaaaa atcgcagaaa aaataaaacg aaatgaagaa ttaccactaa cttttggaga
      241 attaaaaact aaatgggatg aaaaatacaa gccaactgta aaaga
//
LOCUS       DAGCWU010000354         8000 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.129, whole genome shotgun sequence.
ACCESSION   DAGCWU010000354 DAGCWU010000000
VERSION     DAGCWU010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.129"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(134..577)
                     /locus_tag="MBZ71_002863"
     CDS             complement(134..577)
                     /locus_tag="MBZ71_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBT4430729.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="MBZ71_002864"
     CDS             complement(795..2135)
                     /locus_tag="MBZ71_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4430730.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="MBZ71_002865"
     CDS             2404..3300
                     /locus_tag="MBZ71_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBT4430731.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="MBZ71_002866"
     CDS             complement(3450..4391)
                     /locus_tag="MBZ71_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBT4430732.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="MBZ71_002867"
     CDS             4517..5638
                     /locus_tag="MBZ71_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBT4430733.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="MBZ71_002868"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="MBZ71_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBT4430734.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="MBZ71_002869"
     CDS             7426..7992
                     /locus_tag="MBZ71_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4430735.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2656 a         1497 c         1585 g         2262 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttattcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt
//
LOCUS       DAGCWU010000355         5741 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.154, whole genome shotgun sequence.
ACCESSION   DAGCWU010000355 DAGCWU010000000
VERSION     DAGCWU010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.154"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(252..623)
                     /locus_tag="MBZ71_002870"
     CDS             complement(252..623)
                     /locus_tag="MBZ71_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4430736.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            768..953
                     /locus_tag="MBZ71_002871"
     CDS             768..953
                     /locus_tag="MBZ71_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4430737.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1111..1278
                     /locus_tag="MBZ71_002872"
     CDS             1111..1278
                     /locus_tag="MBZ71_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430738.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1896..2555
                     /gene="vanR"
                     /locus_tag="MBZ71_002873"
     CDS             1896..2555
                     /gene="vanR"
                     /locus_tag="MBZ71_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBT4430739.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2558..3901
                     /gene="vanS"
                     /locus_tag="MBZ71_002874"
     CDS             2558..3901
                     /gene="vanS"
                     /locus_tag="MBZ71_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBT4430740.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            4072..4878
                     /gene="vanY"
                     /locus_tag="MBZ71_002875"
     CDS             4072..4878
                     /gene="vanY"
                     /locus_tag="MBZ71_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBT4430741.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            4891..5727
                     /gene="vanW"
                     /locus_tag="MBZ71_002876"
                     /pseudo
     CDS             4891..5727
                     /gene="vanW"
                     /locus_tag="MBZ71_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT         1682 a         1201 c         1419 g         1439 t
ORIGIN      
        1 ccgtcctttc cattcagggg tttggggatt tccccaacaa gcaaaacgga cgcggcccgg
       61 cagggacggg gcaggccgtt tttccgggag tggctacact ccctatgctt gctacgttac
      121 tgtttctcca ctaacaagac gtttccaaag ccccaaacta cccgcaaaat agccctctgg
      181 aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag ctcccgctaa aagtaaaaca
      241 gaggatgcca gttacacatc ctccgtttca ttcttcttcg gcttttcaat tagtatggcg
      301 ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg ggcagagttt cagcttgtgg
      361 ctgacccttt gccgcagttc gctttcctcc cgcatgatct ccgggttaaa gtagcgttcc
      421 acaggaagcc cgcaggcttt aataagctgt atgacaaccg gcagactggg aatggccccg
      481 tcgttttcga tgttggcaag atagcgccag tcaatattta ttttctctgc cattgctttg
      541 cgcgaaacgc gctttgcttg ccgggccgct ttcacatccg cgccgaaagt ttcaaagccg
      601 ggacaatctt caatattcgc catatcgcac ctcccactta cattgtatat ttcatactct
      661 gcccgtggaa tgttggtata tgcacctttc ttaaagttta tatttcacat tttgtttctt
      721 ttattcttcg agcaaaagaa ttataatgta ccctgtggag gtgtactatg gactacatga
      781 cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg atggataaac tactattgtg
      841 ctgcgggacg catccccggc gctgtaaaga tggcgacgat ctggctcatt cccaaaactg
      901 cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc ggttactgaa tagtataatg
      961 aaaccggcgc attttggata tggttttgaa gagagagaaa gcatggtatt ttgtcacgga
     1021 aaaactataa gatgatagat ggcaggctgc tccagaccga taaaaagtat tctcatctga
     1081 aacagaaaga aaagatttac acatggatgt atgaagaagc gaaactttat catgacagaa
     1141 atggaaaatg cccgaaacaa agacgaggaa gctgctgctg tatcagccgt atatggtaga
     1201 attgaaaagg ccggcatctg gattctttat ggtgaagtct ccagacattg gataactgct
     1261 ggtctaatgc gcctttgagt atggggtgat tgattttaat accggggagg tacggctttt
     1321 tgaggaaaat attctgggat ttacatttgg cgattattac atttcctgta actgcaagga
     1381 cacggatgat gaagatatga ggaatataga cttgctgatt ttctactgtc ttgaacggta
     1441 ttaggactga atctgctgcg cggagtgcgg caagagcgac cggagggagt acacacaggc
     1501 cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca aaaaagaggc cgcgcatttt
     1561 gctgaggctt ggggtgtccc caacaagcaa aacgagaacg ccgcaggcgg gcaagtggtg
     1621 gatcagaata tccgtaaagg atattctgat ccattgcttg ccagtattat cgggatgttt
     1681 tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag gtgttaatta ttattatctt
     1741 gatttaattc gtttaaacca ataatataga tacatacacc atgaaaagaa gcgagtatcc
     1801 caatgacgga tgattaaaat ccgaaaagaa atcgctttga aactacaggg aaactacaga
     1861 ctgttatgtt atcttcttaa aatggaggga tttttatgtc gatacgaatt ctacttgtcg
     1921 aggatgatga tcatatctgt aatacagtaa gggcgtttct ggctgaggca ggatatcagg
     1981 tggatgcctg cacagatgga aatgaggcat acaccaagtt ttacgaaaac acttatcaac
     2041 tggttattct tgatattatg ctgcccggta tgaacgggca tgaacttttg cgtgaatttc
     2101 gtgcgaaaaa tgatactccc attctgatga tgacagccct gtcggatgac gaaaaccaaa
     2161 tccgggcgtt tgatctagag gcagacgact atgtaacaaa gccattcaag atgcagattt
     2221 tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc gctggcaaag gaaatccgtg
     2281 tcggcaggct gacacttctg ccggaggatt tcacggtact ttgtgacggt acagagctgc
     2341 ccctgacacg aaaagaattt gaaatccttt tgctgctggt gcagaacaaa ggcagaacct
     2401 taacgcatga aatcattttg tcacgtatat ggggatatga ctttgagagt gatggcagca
     2461 cagtccacac tcatatcaaa aatttgcggg ccaagctgcc ggaaaatatc atcaaaacca
     2521 ttcgcggtgt aggataccga ttggaggaat cataataatg gaaagaaaag ggattttcat
     2581 taaggttttt tcctatacga tcattgtcct gctactgctt gtcggtgtaa cagcaacact
     2641 gtttgcacag caatttgtgt cttatttcag agtgatggaa ttacagcaaa cagtaaaatc
     2701 ctatcagcca ttggtggaac tgattcagaa tagcgatagg cttgatattc aagaggtggc
     2761 agggctgttt cactacaata accaatcctt tgagttttat attgaagata aagagggaag
     2821 cgtactctat gccacaccaa atgccaatac atcaaatagt tttagacccg actttcttta
     2881 tgtggtacat agagatgata atatttcgat tgttgctcaa agcaaggcag gtgtgggatt
     2941 gctttatcaa gggctgacaa ttcggggaat tgttatgatt gcgataatgg ttgtattcag
     3001 ccttttatgc gcgtatatct ttgcgcggca aatgacaacg ccgatcaaag ccttagcgga
     3061 cagtgcgaat aaaatggcaa acctgaaaga tgtaccgccg ccgctggagc gaaaggatga
     3121 gcttggcgca ctggctcatg atatgcattc catgtatgtc aggctgaaag aaaccatcgc
     3181 aaggctggag gatgaaatcg caagggaaca tgagttggag gaaacacagc gatatttctt
     3241 tgcggcagct tctcatgagc taaaaacgcc catcgcggct acaagcgttc tgttggaggg
     3301 aatgcttgaa aatatcggtg actacaaaga tcattctaag tatctgcgcg aatgcatcaa
     3361 aatgatggat aggcagggca aaatcatttc cgaaatactg gagcttgtca gcctgaatga
     3421 tgggagaatc gtacccatag ctgaaccgtt ggacataggg cgcacggttg ccgagttgct
     3481 gcccgatttt caaaccttgg cagaggcaaa caaccagcgg ttcgtcacag atattccagc
     3541 cgggcaaatt gtcctgtccg atccgaggct gctccaaaag gcactatcca atgtcatatt
     3601 gaatgcagtt cagaacacgc cgcagggagg cgaggtacgg atatggagtg agcctggtgc
     3661 tgaaaaatgc cgcctttttg ttttgaacat gggcgttcac attgatgata ctgcgcttcc
     3721 aaggctgttc accccattct atcgcattga tcaggcgcga agcagaaaaa gtgggcgaaa
     3781 cggtttagga cttgccatcg tacaaaaaac gctggatgcc atgagcctcc agtatgcgct
     3841 ggaaaacacc tcggatggcg ttttgttctg gctggattta ccgctcacat caacattgta
     3901 aatatttaaa acttaaatga ttttgaccga caggtataac cctgccggtc tttttttgct
     3961 gctacaggaa aactacagac tggctacagg gaaagtacag atgcgcttgc cataataaca
     4021 atcgtaccag ccacaaatcg tagttttatt gcaaaggagg cattcgatca aatggaaaaa
     4081 agcaactatc attccaatgc ggatcatcac aaacggcata tgaaacaatc cgtggaaaag
     4141 cgggcttttc tatgtgcgtt cattatttcg ttcacagtct gcacgctgtt tttggggcgg
     4201 agactggctt ccgtattgga ggcaacacag ataccgccca tccctgcaac tcatacaggc
     4261 agcagcactg acgtagtgga gaatttggag gaaaacgctc ttgccaccgc caaagaacag
     4321 ggagatgaac aggaatggag cctgatttta gtgaacaggc agaaccccat ccccgcacag
     4381 tacgatgtgg agcttgagca actatcaaat ggtgagcgga tagatattcg gatttctccc
     4441 tatcttcaag atttgtttga tgccgcaaga actgatggag tttacccgat tgtcgcatcc
     4501 ggataccgaa caacagaaaa acagcaagaa attatggatg aaaaaattgc cgaatataag
     4561 gcgaaaggct acacctctgc acaggctaaa gcggaagcag aaacttgggt ggccgtgccg
     4621 ggaacgagcg agcatcagct tggtcttgct gtggatatca atgcggacgg aattcattca
     4681 acaggcaacg aggtttatag atggctggat gaaaacagct atcgttttgg ttttattcgt
     4741 cgctacccac cagacaagac agagataacc ggtgtgagca acgagccgtg gcattaccga
     4801 tatgttggca tcgaagctgc cacagagatg tacaaccaag gggtttgcct tgaggaatat
     4861 ttaaaaccag aaaaatgaga aaaggatgtt atggacagaa aaagattgac acagcgtttc
     4921 ccgttcctgc ttccaatgag acgggcgcag agaaaaatgt gcttttatgc gggaatgaga
     4981 tttgacggct gtcgctatgc gcagacgata ggggaaaaat cgctttccca tttgctcttt
     5041 gaaacggatt gcgcattata caaccacaat accggatttg acatgatata ccaggaaaac
     5101 aaggtgttca acttaaagct ggcagcaaag accttaaacg gcttattgat aagaccgggg
     5161 gaaacctttt ctttctggtg gctggtacgc catgcggaca aagatacccc ctataaagac
     5221 ggccttacgg tgaccaatgg taaactcacc accatgtcgg gcggcggtat gtgccagatg
     5281 agcaatttac tattttggat gttcctgcat acgccattga caattatcca gcgcagaggt
     5341 cacgaagtaa aggagtttcc agagccaaac agtgacgaga ttaaaggggt ggatgcaacc
     5401 atttcagagg gctggattga tttaaaagtg cgaaacgata ccgactgcac ctaccaaata
     5461 tgagtgacct tagatgatga gaaaatcatc ggtcaggtgt ccgccgacaa agagccccaa
     5521 gcattataca aaattacaaa tggcagtatc cagtatgtcc gtgaaagtgg cgggatttat
     5581 gaatatgccc aggttaaacg gatgcaagtt gccttaggta ccggggaaat aatagattgc
     5641 aagctgcttt atacaaacaa atgcaaaatc tgttatcccc tcccggaaag tgtggatatt
     5701 cagtctaatg gtgataagtc aagatgaaat ttaagatttt c
//
LOCUS       DAGCWU010000356         4945 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.168, whole genome shotgun sequence.
ACCESSION   DAGCWU010000356 DAGCWU010000000
VERSION     DAGCWU010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.168"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            79..543
                     /locus_tag="MBZ71_002877"
     CDS             79..543
                     /locus_tag="MBZ71_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4430742.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            527..1156
                     /locus_tag="MBZ71_002878"
     CDS             527..1156
                     /locus_tag="MBZ71_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBT4430743.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1510..2988
                     /gene="msr(C)"
                     /locus_tag="MBZ71_002879"
     CDS             1510..2988
                     /gene="msr(C)"
                     /locus_tag="MBZ71_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBT4430744.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3035..3481
                     /locus_tag="MBZ71_002880"
     CDS             3035..3481
                     /locus_tag="MBZ71_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBT4430745.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3680..4195)
                     /locus_tag="MBZ71_002881"
     CDS             complement(3680..4195)
                     /locus_tag="MBZ71_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4430746.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4312..4945
                     /locus_tag="MBZ71_002882"
                     /pseudo
     CDS             4312..4945
                     /locus_tag="MBZ71_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1582 a          827 c         1058 g         1478 t
ORIGIN      
        1 tctttgactt ttttcttttt ttattctatt attgacaagt caatcgttga cgagtcaatt
       61 attagaaagg aggaaattat gtctttagaa gagattagtc accttttata tcaaatcaaa
      121 ttagccgatc aaaaaatcgt ccagatcttt gaagaaaata tcggtataag tcttactaga
      181 tacgagatca tgatgagact gaaagaagaa aatccattgc tacaaagcag tttgcaagaa
      241 aagttgcaga ttgatcaagg agcagttact cgtcatctga aggtattgga agcaaatggc
      301 tatgtttcgc gtcagagaaa tcctcagaac aaccgggaag tgtatgtagc gctaacagaa
      361 aaagcccaga agaaaatcga gcattgtgaa atggaccata aagatacagg ttccgtgcta
      421 tccccggctt tcagcgatgt agaaatgaaa caatttctaa cacttttaga taaattgaat
      481 acccaacttt gcaatataac aacagaaacg aaagaggtta ataataatgg aacaaaaaaa
      541 tgatttttca acaattatga ctggtagaag atccgttcgc gtatatgatg aaacatacaa
      601 aatcccacac gaagaaatgc tggaaatgat caaagaagcg acgactgcac cttcttctgt
      661 caatatgcaa ccatggcgct ttgtggtcgc tgaaagcgag gaagcaaaag aaatcttgcg
      721 tcctttgatc cgtttcaata cgagacaaaa tgatacttct tctgcaatgg tcatgatttt
      781 tggcgatatg caatgttacg aatatggcga agaaatctat aaccaagctt acgaatcagg
      841 gaagatgcca aaagaagtca gagatcaaca attagctgcg atcattcctt attacaagag
      901 tttttcaaga gaagaaatga acgatgtagt caaagtcgat tctagcttag cggctatgca
      961 attcatgtta gttgcgcgtg atcatggcta tgaaacaaat ccaattggtg gattcgaagc
     1021 agatcagttg gcagaggcat ttggtttgga taaagaccgt tatgttccag ttattatttt
     1081 atcaatagga aaagcggtag aagaaggtta cgagtctgtt cgtttagcac cagaaaagat
     1141 cactacgttc gaataaaagg ttttcagtta ctattcagta gctattgctt tttttgaatc
     1201 gtgtgtggta acctaataaa aaaggaggtc ggaaaaatga ctgcatcgat gaaattacgt
     1261 ttcgaacttt tgaataacaa ctaaataaat tcaaaagaca tgtataaaca acaatttcat
     1321 ggatggagta tgtccatttt ttcgaacgac agtagaatag gtgaaagcta tgacagattt
     1381 ggtgatttag tcatagttat ttcgttcaga gtaggaaaag atgtgatgca gtttttttgc
     1441 aaacagattt ttctgctctt ttttagtatc gataattgtt gttttacatg aaataggagt
     1501 gtaaaacata tggaaaattt agcagtaaat ataacaaatc tacaagtcag ctttggcaac
     1561 cagctagaac tatctattga ttctcttcgt gtctatcagc aagatcgtat agggatcata
     1621 ggagaaaatg gtgtcggtaa atcgactttg ctcaaactaa tagccggtga actttttccc
     1681 gatcatggga aaatccaaac agagatcacc ttcaactacc tgcctcaatt aacctatctc
     1741 gctgaagcaa aggacctaaa tttggaatta gccagtcatt tccagttaag actggaagaa
     1801 acttcagagc ggaaatggag cggaggggaa gaacgaaaga tcgagttgat acgtcttctt
     1861 tcttcttatg aacaagggat gcttctagac gagccaacaa cccatctaga tagaaaaagt
     1921 attgatcgac tgattgaaga gcttcgttat tattatggta cgctggtttt tgttagtcat
     1981 gatcgctatt ttctagatga gctagcatcg aaaatctggg aagtaaaaga cggagaaatc
     2041 cgagagtttt cggggaatta tagtgcctat ctcactcaaa aggaattgga gaaaaagact
     2101 cagctacgag aagcagagtc gatcatgaaa gagaaaaaac gattggaaaa atcgatccaa
     2161 gaaaagaaaa aacaagcgga aaagttggaa aaagtgtcca gtaaaaagaa aaagcaacaa
     2221 atcagaccgg atcggttgtc ttcctctaaa caaaaagaca gtgtacaaaa agccatccaa
     2281 aagaatgcga aaacattaga gagaagactc caaaaaatag gagaaacaac caagccgcag
     2341 cagatgaaac aaatccgttt tccagtacca aaatctcttg aactccacag ccgctatccc
     2401 atcatgggac aaaatgtcca attggaaaga agtgggagaa cattactggt aaatggtgat
     2461 tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg aaaatggttc aggtaagaca
     2521 accttattag aacatatccg caaacaagga gaaggaatcc ttctctctcc gaaagtaagc
     2581 tttcaagtat atcagcaaaa gggttatcaa atgacatctg aagaatccat cattcgtttt
     2641 gtcatgagac aaacagagtt ttcagaatcg cttgtccgta gtttgctgaa tcacttaggg
     2701 tttgctcagg aaactttgac gaaaccgtta tgtacattaa gtgggggaga agcgacccgt
     2761 ctgacgattg ctttgctttt tactaagcca agtaatgtgt tgctgttaga tgaaccgact
     2821 aattttattg atatggcaac gatcgaagct ttagagaagc taatgcaaat atatccggga
     2881 acgattttgt ttacttctca tgattcttac tttgtcgagc gtacggctga tgaagtttat
     2941 gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa atttttaaat gtgctacgat
     3001 gattttcgtg acaaatttaa aggagtggta cagattgcct acaaataaaa aagaaggaat
     3061 aatttttaca acaatgatgt gttttttgat ggtcttgggg atgagcgcgt ataatttatg
     3121 gctgcatcaa gatcttcatt tatcaagttt gttgattggt ctagtgcctg gattcgtggt
     3181 ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg aaaaaaattg cctttaaact
     3241 accagtaaat ccagaaagca aggtccaaat gatcttaact atttcatgct tgatggtttt
     3301 aggaatggtg acatgtatgt ctttattcgg tgtgatcatg gaacaaggaa tacctgatca
     3361 attattctct gcttatatga atgcttggaa aatgaatgtg attgccgctt taccgttgca
     3421 gttgatcatc gttggcccgc tttcaagaag tgtgctaaaa gcaattcaaa gtaatgaatg
     3481 atggaacact gttatttgaa aaaaggcctt ggcatgcttt ttgtcatagt ctcttgaatg
     3541 cagttctaga aaaatagtga tccgaacgat aggttttggc ctgtcgttcg gatcgctttt
     3601 tcttttccaa ctgtattgct cttttattga agataatttg ctcttcaata gatatttcta
     3661 gaaaggttct gtttatccct tactcaaaaa actgctgcat ttgttctctt acttgctgac
     3721 accattggag ttcctgctga caaagcataa ttctgcgctg gatcacaaca taacgtccaa
     3781 attcttgttt cttgtctagc gagaggtcat tcaatttttt ttgatttttt tgcatgattt
     3841 tgttcagctg ttgttctcta gtgaatagca gtccagtagc tgttgtttta tcgagcgtcg
     3901 aaaaagcata gatttttgct agaaattcat ctttttgcgt aggagcggga gtttcctctt
     3961 tgatccattc ctctacaaga gcacgtcctt catcagtcaa gtggtagatc tttttttgag
     4021 agtgctcgtc tttgtccaaa acggtcacat aaccctgctg gtctaacttg gcaagcgttg
     4081 tatagatctg actgtgatgg gcttcccaaa aaattgtgat cagctgcttc aattcatacc
     4141 cagaacgtgg tttggataaa agcacgcata ataatatata ggctaaagta ttcataaaaa
     4201 tcctacctct cgtcttattt tctttattat atcacagtaa agtttccacg ttttacatgt
     4261 gcatatttac atgtaaaata aatcatgtat gatgctaata gagagaaagg gatggaaata
     4321 ttggagaatc aaatagatat acaagaacaa acaaatgaag aaacaaaggg gaaaagttca
     4381 gcgctagttg tagcagctac ctgtatgatc gcttttatgg gagtgggtct agtagaccca
     4441 atcttaaaaa cgattgctgc acagttaaac gctacgccag ctgaaacgac cctgttattt
     4501 actagttaca tgttagtcac tggtgtggtg atgctattta caggtttctt atctagtcgg
     4561 atcggtttga aaaaacattg atgacaggat tgtttatcat cgttgtattc gctggtcttg
     4621 gcggattatc tgggaatatc ggaatgctga ttgggttacg tgccggctgg ggtgtcggga
     4681 atgctttatt tgtctctact gctttagcaa cgatcgtcag tattttagtt ggtaacacag
     4741 aaaaagcaat catgatgtat gaagcagcac ttggctgcgg aatggctgtt ggccctctag
     4801 tcggtggggt cttgggcagt ggttcatggc gttatccatt ttttggggta gcatttttga
     4861 tgttttgcgg ttttatagcc ctagcggtac ttttacctga aactgagaaa ccaaagagaa
     4921 aagtaggcct ttgggaaggt gtcaa
//
LOCUS       DAGCWU010000357         4945 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.169, whole genome shotgun sequence.
ACCESSION   DAGCWU010000357 DAGCWU010000000
VERSION     DAGCWU010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.169"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            79..543
                     /locus_tag="MBZ71_002883"
     CDS             79..543
                     /locus_tag="MBZ71_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4430747.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            527..1156
                     /locus_tag="MBZ71_002884"
     CDS             527..1156
                     /locus_tag="MBZ71_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBT4430748.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1510..2988
                     /gene="msr(C)"
                     /locus_tag="MBZ71_002885"
     CDS             1510..2988
                     /gene="msr(C)"
                     /locus_tag="MBZ71_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBT4430749.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRFEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3035..3481
                     /locus_tag="MBZ71_002886"
     CDS             3035..3481
                     /locus_tag="MBZ71_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBT4430750.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3680..4195)
                     /locus_tag="MBZ71_002887"
     CDS             complement(3680..4195)
                     /locus_tag="MBZ71_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4430751.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4312..4945
                     /locus_tag="MBZ71_002888"
                     /pseudo
     CDS             4312..4945
                     /locus_tag="MBZ71_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1582 a          827 c         1057 g         1479 t
ORIGIN      
        1 tctttgactt ttttcttttt ttattctatt attgacaagt caatcgttga cgagtcaatt
       61 attagaaagg aggaaattat gtctttagaa gagattagtc accttttata tcaaatcaaa
      121 ttagccgatc aaaaaatcgt ccagatcttt gaagaaaata tcggtataag tcttactaga
      181 tacgagatca tgatgagact gaaagaagaa aatccattgc tacaaagcag tttgcaagaa
      241 aagttgcaga ttgatcaagg agcagttact cgtcatctga aggtattgga agcaaatggc
      301 tatgtttcgc gtcagagaaa tcctcagaac aaccgggaag tgtatgtagc gctaacagaa
      361 aaagcccaga agaaaatcga gcattgtgaa atggaccata aagatacagg ttccgtgcta
      421 tccccggctt tcagcgatgt agaaatgaaa caatttctaa cacttttaga taaattgaat
      481 acccaacttt gcaatataac aacagaaacg aaagaggtta ataataatgg aacaaaaaaa
      541 tgatttttca acaattatga ctggtagaag atccgttcgc gtatatgatg aaacatacaa
      601 aatcccacac gaagaaatgc tggaaatgat caaagaagcg acgactgcac cttcttctgt
      661 caatatgcaa ccatggcgct ttgtggtcgc tgaaagcgag gaagcaaaag aaatcttgcg
      721 tcctttgatc cgtttcaata cgagacaaaa tgatacttct tctgcaatgg tcatgatttt
      781 tggcgatatg caatgttacg aatatggcga agaaatctat aaccaagctt acgaatcagg
      841 gaagatgcca aaagaagtca gagatcaaca attagctgcg atcattcctt attacaagag
      901 tttttcaaga gaagaaatga acgatgtagt caaagtcgat tctagcttag cggctatgca
      961 attcatgtta gttgcgcgtg atcatggcta tgaaacaaat ccaattggtg gattcgaagc
     1021 agatcagttg gcagaggcat ttggtttgga taaagaccgt tatgttccag ttattatttt
     1081 atcaatagga aaagcggtag aagaaggtta cgagtctgtt cgtttagcac cagaaaagat
     1141 cactacgttc gaataaaagg ttttcagtta ctattcagta gctattgctt tttttgaatc
     1201 gtgtgtggta acctaataaa aaaggaggtc ggaaaaatga ctgcatcgat gaaattacgt
     1261 ttcgaacttt tgaataacaa ctaaataaat tcaaaagaca tgtataaaca acaatttcat
     1321 ggatggagta tgtccatttt ttcgaacgac agtagaatag gtgaaagcta tgacagattt
     1381 ggtgatttag tcatagttat ttcgttcaga gtaggaaaag atgtgatgca gtttttttgc
     1441 aaacagattt ttctgctctt ttttagtatc gataattgtt gttttacatg aaataggagt
     1501 gtaaaacata tggaaaattt agcagtaaat ataacaaatc tacaagtcag ctttggcaac
     1561 cagctagaac tatctattga ttctcttcgt gtctatcagc aagatcgtat agggatcata
     1621 ggagaaaatg gtgtcggtaa atcgactttg ctcaaactaa tagccggtga actttttccc
     1681 gatcatggga aaatccaaac agagatcacc ttcaactacc tgcctcaatt aacctatctc
     1741 gctgaagcaa aggacctaaa tttggaatta gccagtcatt tccagttaag actggaagaa
     1801 acttcagagc ggaaatggag cggaggggaa gaacgaaaga tcgagttgat acgtcttctt
     1861 tcttcttatg aacaagggat gcttctagac gagccaacaa cccatctaga tagaaaaagt
     1921 attgatcgac tgattgaaga gcttcgttat tattatggta cgctggtttt tgttagtcat
     1981 gatcgctatt ttctagatga gctagcatcg aaaatctggg aagtaaaaga cggagaaatc
     2041 cgagagtttt cggggaatta tagtgcctat ctcactcaaa aggaattgga gaaaaagact
     2101 cagctacgag aagcagagtc gatcatgaaa gagaaaaaac gatttgaaaa atcgatccaa
     2161 gaaaagaaaa aacaagcgga aaagttggaa aaagtgtcca gtaaaaagaa aaagcaacaa
     2221 atcagaccgg atcggttgtc ttcctctaaa caaaaagaca gtgtacaaaa agccatccaa
     2281 aagaatgcga aaacattaga gagaagactc caaaaaatag gagaaacaac caagccgcag
     2341 cagatgaaac aaatccgttt tccagtacca aaatctcttg aactccacag ccgctatccc
     2401 atcatgggac aaaatgtcca attggaaaga agtgggagaa cattactggt aaatggtgat
     2461 tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg aaaatggttc aggtaagaca
     2521 accttattag aacatatccg caaacaagga gaaggaatcc ttctctctcc gaaagtaagc
     2581 tttcaagtat atcagcaaaa gggttatcaa atgacatctg aagaatccat cattcgtttt
     2641 gtcatgagac aaacagagtt ttcagaatcg cttgtccgta gtttgctgaa tcacttaggg
     2701 tttgctcagg aaactttgac gaaaccgtta tgtacattaa gtgggggaga agcgacccgt
     2761 ctgacgattg ctttgctttt tactaagcca agtaatgtgt tgctgttaga tgaaccgact
     2821 aattttattg atatggcaac gatcgaagct ttagagaagc taatgcaaat atatccggga
     2881 acgattttgt ttacttctca tgattcttac tttgtcgagc gtacggctga tgaagtttat
     2941 gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa atttttaaat gtgctacgat
     3001 gattttcgtg acaaatttaa aggagtggta cagattgcct acaaataaaa aagaaggaat
     3061 aatttttaca acaatgatgt gttttttgat ggtcttgggg atgagcgcgt ataatttatg
     3121 gctgcatcaa gatcttcatt tatcaagttt gttgattggt ctagtgcctg gattcgtggt
     3181 ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg aaaaaaattg cctttaaact
     3241 accagtaaat ccagaaagca aggtccaaat gatcttaact atttcatgct tgatggtttt
     3301 aggaatggtg acatgtatgt ctttattcgg tgtgatcatg gaacaaggaa tacctgatca
     3361 attattctct gcttatatga atgcttggaa aatgaatgtg attgccgctt taccgttgca
     3421 gttgatcatc gttggcccgc tttcaagaag tgtgctaaaa gcaattcaaa gtaatgaatg
     3481 atggaacact gttatttgaa aaaaggcctt ggcatgcttt ttgtcatagt ctcttgaatg
     3541 cagttctaga aaaatagtga tccgaacgat aggttttggc ctgtcgttcg gatcgctttt
     3601 tcttttccaa ctgtattgct cttttattga agataatttg ctcttcaata gatatttcta
     3661 gaaaggttct gtttatccct tactcaaaaa actgctgcat ttgttctctt acttgctgac
     3721 accattggag ttcctgctga caaagcataa ttctgcgctg gatcacaaca taacgtccaa
     3781 attcttgttt cttgtctagc gagaggtcat tcaatttttt ttgatttttt tgcatgattt
     3841 tgttcagctg ttgttctcta gtgaatagca gtccagtagc tgttgtttta tcgagcgtcg
     3901 aaaaagcata gatttttgct agaaattcat ctttttgcgt aggagcggga gtttcctctt
     3961 tgatccattc ctctacaaga gcacgtcctt catcagtcaa gtggtagatc tttttttgag
     4021 agtgctcgtc tttgtccaaa acggtcacat aaccctgctg gtctaacttg gcaagcgttg
     4081 tatagatctg actgtgatgg gcttcccaaa aaattgtgat cagctgcttc aattcatacc
     4141 cagaacgtgg tttggataaa agcacgcata ataatatata ggctaaagta ttcataaaaa
     4201 tcctacctct cgtcttattt tctttattat atcacagtaa agtttccacg ttttacatgt
     4261 gcatatttac atgtaaaata aatcatgtat gatgctaata gagagaaagg gatggaaata
     4321 ttggagaatc aaatagatat acaagaacaa acaaatgaag aaacaaaggg gaaaagttca
     4381 gcgctagttg tagcagctac ctgtatgatc gcttttatgg gagtgggtct agtagaccca
     4441 atcttaaaaa cgattgctgc acagttaaac gctacgccag ctgaaacgac cctgttattt
     4501 actagttaca tgttagtcac tggtgtggtg atgctattta caggtttctt atctagtcgg
     4561 atcggtttga aaaaacattg atgacaggat tgtttatcat cgttgtattc gctggtcttg
     4621 gcggattatc tgggaatatc ggaatgctga ttgggttacg tgccggctgg ggtgtcggga
     4681 atgctttatt tgtctctact gctttagcaa cgatcgtcag tattttagtt ggtaacacag
     4741 aaaaagcaat catgatgtat gaagcagcac ttggctgcgg aatggctgtt ggccctctag
     4801 tcggtggggt cttgggcagt ggttcatggc gttatccatt ttttggggta gcatttttga
     4861 tgttttgcgg ttttatagcc ctagcggtac ttttacctga aactgagaaa ccaaagagaa
     4921 aagtaggcct ttgggaaggt gtcaa
//
LOCUS       DAGCWU010000358         4539 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.175, whole genome shotgun sequence.
ACCESSION   DAGCWU010000358 DAGCWU010000000
VERSION     DAGCWU010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.175"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..134
                     /locus_tag="MBZ71_002889"
     CDS             <1..134
                     /locus_tag="MBZ71_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4430752.1"
                     /translation="IGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            369..605
                     /locus_tag="MBZ71_002890"
     CDS             369..605
                     /locus_tag="MBZ71_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430753.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            612..2564
                     /locus_tag="MBZ71_002891"
     CDS             612..2564
                     /locus_tag="MBZ71_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBT4430754.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2636..3133)
                     /gene="dfrG"
                     /locus_tag="MBZ71_002892"
     CDS             complement(2636..3133)
                     /gene="dfrG"
                     /locus_tag="MBZ71_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBT4430755.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3495..4535
                     /locus_tag="MBZ71_002893"
     CDS             3495..4535
                     /locus_tag="MBZ71_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4430756.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
BASE COUNT         1583 a          737 c          815 g         1404 t
ORIGIN      
        1 atatagggat tgataacctt atagaagtta ttactaataa attttattca tcaacacatc
       61 gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga atatacaaaa aaaagacaac
      121 gtcttgcata ttaagtagtt cagttttgga gtacaaaaag tgaaaatgta aacctaaatg
      181 agtatcaaat tgcaactgaa tttgatactc gttttttatt gttaaatcaa cgtttgttgt
      241 taatttacac cacaaaatgt aagttaaaat gtatataaaa atgcgaacgt gaaataatta
      301 ttgtaggaat gcaaattggt gtgttaaaat ggttacaatt tactaagcaa aaaggggtgc
      361 attttcctat gcaatttctt gagccaaaaa ataagaacgc taagtctgtg gattgggaaa
      421 tatcagagca agtaagggtg atagttaagc aatacgctga atatgcagag cgtactgaaa
      481 gcgaagcagt ggatgaattc ttattgaaca ttttagacga taagaagttt attgaatgga
      541 ttgccaacaa gcgaagtaat aagcggattg ttgaaaaaat gggtatcaaa gatagagtgg
      601 gttgatagac aatgaccaag cttaaaagat tagccacgaa ggaagatgaa attgtagatg
      661 ttaaaattcc gatttcaaac gaagaattaa aagatagggc taaacaatat gatttattaa
      721 ctccaaaacg atttgcaaca aggtataaca aaatgttgtt cttgccaacg tcttttaaat
      781 ggaacggttc tgaatatccc attcaatata attactgcat caatccgttt tgttgtaatt
      841 ttgggaaaga acagcacaaa ttcaaagatg taaaagggaa accaagtcga tataaaatga
      901 ctggtagttc caaagataaa ggtcataaag gtatgtactg caatgacaat cctattggca
      961 gaggtgtatc gcaaaactgt acagtaaccc ctttatctaa ttggtctgtt gttgaagaaa
     1021 ttaaacgcct tatagaaatc aatagcatcc aagatgttga gccagattat caatttcata
     1081 aagaaggctg ctcggaagaa gaatcaaccc cattcaatga gccgaagcaa ttttacaaaa
     1141 gggggaaaag taggggcaaa tctcaacgct atcaatgtaa ggcgtgtaag aagttcacta
     1201 atgttctacc aaaaagagaa gaaaccacta cttatcacca acaaaagaac accattcttc
     1261 caatgttcgc taagatggtt gttggaaggg tttcagtcag tcgtacttgt gatattttag
     1321 gaattggagt tggaacctac tatcataagc tggagtggct gtataggcgt tgtttggagt
     1381 ttttagaacg atatgagaca caaccccttc aaacaaagaa atttaacgag atgtggctta
     1441 atacggacaa gatgcactac tacctaaata acgtccgtaa gaaaggacaa ggttcaaaga
     1501 aatatacggg gtttgaagac cttaatatgc aaacgtatat agttgtatct gctgaggtct
     1561 tatcaagata tgtgttccgt tctgatgttg cctatgattg gaatatttca atggatgaat
     1621 taaatgaaga tacaagaaaa ttcaaagaag accatctcaa tacttttagt cgtaaaaatg
     1681 ataggttaga ttggtcttat tatccacaag aaccatccgc taacgatagc gaaaatagga
     1741 atgcttatct tcacgaatta gggaaaatta ctaataggag tagatttgtc gatggtttaa
     1801 atgttgatgc tccttatact acaactgctc actattggtt aattaaacaa atggttaatg
     1861 ccgacgaatg gcgaatgatt agtgatgatg acttctctat tagaaacgcc ttttatagag
     1921 tattcacgaa agaattaagg ttgagcgatg cacatcattt tatttgccaa gttaacaaga
     1981 cgaaaagcag aaaacaatgc ttaaaagaat tcggacaagc aaaagcagaa ctgctcgatt
     2041 ggggagatat cagaggattc aaaacaaagt ttctacggac tttggcatct cattatctaa
     2101 ctgaattact aacaagtcat caattccacg aagaggctat tagtaaagat ggagaaagat
     2161 atcgcaaata tgccgataac cctatcaaac atccattagc cactaaggat aaaggatttt
     2221 attcagttga ttgtagaacg gatttatctg ctctggaacc aaacgaaatc gcaaagatgc
     2281 tattaaatgt taatgaccat tcgaccaata gctttattca acagataaga cgttatatat
     2341 catctcttga aagaccatta acaacagctc gtggtgataa gaagagctat atttacgcta
     2401 acttcaatcc gaagtatgct caatttgcga ttacaattct aagaacttac tacaactttt
     2461 gtcgcccttt taaatcagcg gataaaaagg ttttgacacc tgctcaaaga ttagggatta
     2521 cagataaaca atttgattgg aaagatataa tttatttcaa atgacaaaaa gagcttggag
     2581 taatcccaag ctcttgtgct ttatctttac tgttcttctt caattaacgc acccgttaac
     2641 tcaataagtt ttttctttca tatacatgaa aatagtagtt atacggattt ttatcattct
     2701 ttatcccttt ttgggcaaat acctcattcc attcctcata attcacttct ggaaaaaaag
     2761 tatctccttc gaattcatga tgtatttttg tgatgtacat tttctcaaca taagggaaaa
     2821 acaaattata aatctgttct cctccgaaaa taaaaatttc ttcttcgttt ttacataact
     2881 caaaaacatc ttctattgaa tgaacaattt cacaaccatt aaaggtaaac cccttatctc
     2941 tcgtcagaat aatatttctt ctgtcaggta aggctcttcc gattgattca aggttcttcc
     3001 tacctaatat tatcggatgt ccctttgtag tatttttaac atattcccag tccttgggaa
     3061 tcctccaagg aatgtcattc tctttgccaa tcactctatt cttatccatc gcagcaatca
     3121 aagaaacttt catttataac acagcctttt taactaattt accatctaat ttcttatttc
     3181 tatcaaaaaa ccttttctca ttatcctgcc cgttagttga ataagcaatt ttttctataa
     3241 gttatattat cataatacca caaaatcaca aattttcatt caattatcat tttcaattgc
     3301 attttataaa tcattttgta tgtaatttgg tatgtaagtt aaaatgaaaa aagtagtcca
     3361 ttttgaagta cattttgtca ttttgtactc caaaactgaa ctacttactt gcatatatac
     3421 gcctttatag tggagtacta catttacgag attcggttag agtatcagaa aaagaaaaaa
     3481 taaaagttac agaaatgtat acttcaataa atggtgaatt atgtaagatt gatagagctt
     3541 attctggaga aattgttatt ttgcaaaatg agtttttgaa gttaaatagt gttcttggag
     3601 atacaaaact attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa
     3661 ctgttgaacc gagtaaacct gaacagagag aaatgttgct tgatgccctt ttggaaatct
     3721 cagatagtga tccgcttcta cgatattacg tggattctac gacacatgaa attatacttt
     3781 ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg
     3841 tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag
     3901 aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg
     3961 tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact
     4021 taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat
     4081 tatatggttg gaatgtgacg gattgtaaaa tctgttttaa gtacggttta tactatagcc
     4141 ctgttagtac tccagcagat tttcggatgc ttactcctat tgtactggag caagccttta
     4201 gaaaagctgg aacagaattg ttagagccat atcttagttt taaagtttat gcaccacagg
     4261 aatatctttc acgggcatat aacgatgctc ccaaatattg tgcaaatatc gtaaatactc
     4321 aactgaaaaa taatgaggtc attattattg gagaaattcc tgctcgatgt attcaagatt
     4381 atcgcaatga tttaactttt tttacaaatg ggcttagtgt ttgtttagca gagctaaaag
     4441 gatatcaggt taccactggc gaacctgttt gccagacccg tcgtctaaat agtcggatag
     4501 ataaagtaag atatatgttc aataaaataa cttagtgcg
//
LOCUS       DAGCWU010000359         3026 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.207, whole genome shotgun sequence.
ACCESSION   DAGCWU010000359 DAGCWU010000000
VERSION     DAGCWU010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.207"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            47..1018
                     /locus_tag="MBZ71_002894"
     CDS             47..1018
                     /locus_tag="MBZ71_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBT4430757.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1011..2039
                     /gene="vanB"
                     /locus_tag="MBZ71_002895"
     CDS             1011..2039
                     /gene="vanB"
                     /locus_tag="MBZ71_002895"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBT4430758.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2045..2653
                     /locus_tag="MBZ71_002896"
     CDS             2045..2653
                     /locus_tag="MBZ71_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBT4430759.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(2719..2892)
                     /locus_tag="MBZ71_002897"
     CDS             complement(2719..2892)
                     /locus_tag="MBZ71_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430760.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT          814 a          635 c          828 g          749 t
ORIGIN      
        1 gaataaaaac tattagacaa atgatatttt acgaggagga gaaccaatga gaaaaagtgt
       61 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
      121 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
      181 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
      241 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
      301 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
      361 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
      421 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
      481 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
      541 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
      601 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
      661 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
      721 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
      781 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
      841 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
      901 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
      961 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     1021 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     1081 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     1141 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     1201 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     1261 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg
     1321 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     1381 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     1441 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     1501 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     1561 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     1621 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     1681 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     1741 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttcac gcagacattc
     1801 cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat
     1861 gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg
     1921 aggtcaatac catgcccggt tttacatcgt acagccgcta cccacgtatg gtggccgccg
     1981 caggaatcac gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat
     2041 aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc
     2101 caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg
     2161 catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc
     2221 gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt
     2281 cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat
     2341 agagcgagcc gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg
     2401 aagcgcaatt gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag
     2461 taacttcgat tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga
     2521 ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg
     2581 ctttgaatgg tggcactata agttgattga tgagccatac cccgatacct attttaattt
     2641 tgccgtttca taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg
     2701 ctggtcatgg acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa
     2761 attatagtat tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg ggcagacctt
     2821 tcccacgccc attttgtatt ttgctggcgg cggttttgag aaatcaaggc cgccgttctt
     2881 ttctggcagc aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt tttgctattg
     2941 ccatgctcta atccacttcc accactcaaa aaagccttgc ccctccatga actgaacccc
     3001 aaaagttgaa ctatttaatg gactgt
//
LOCUS       DAGCWU010000360         2573 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.221, whole genome shotgun sequence.
ACCESSION   DAGCWU010000360 DAGCWU010000000
VERSION     DAGCWU010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.221"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..111
                     /locus_tag="MBZ71_002898"
     CDS             <1..111
                     /locus_tag="MBZ71_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBT4430761.1"
                     /translation="LKKNGVNCVIRKEHGTDIDAACGQLRSKQMKKTVAK"
     gene            complement(304..1407)
                     /locus_tag="MBZ71_002899"
     CDS             complement(304..1407)
                     /locus_tag="MBZ71_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4430762.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1960..2508
                     /gene="aac(6')"
                     /locus_tag="MBZ71_002900"
     CDS             1960..2508
                     /gene="aac(6')"
                     /locus_tag="MBZ71_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBT4430763.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          836 a          450 c          500 g          787 t
ORIGIN      
        1 ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga tattgatgca
       61 gcttgcggac aattgcgaag caagcaaatg aaaaaaacag ttgccaaata atcgtatcct
      121 actcgttttt tcaattacca taaaaggagc agttttctta attaaggaac tgttcaactt
      181 aaataagaga aaagattcta tagcgaaagc tataaagctt tttttgacaa gaaaaaccct
      241 catcaagcaa agaaatgtcc tcaatttgat gacagcctgc ttgatggggg ttttagcaat
      301 tatttaactg attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac
      361 gtcaagaatg gcctgtttaa atttttctcg ggtgatttca taaggcacat gtttaatatc
      421 atttgttgcc atggctttat ctaagattgc ttctaaatcg gtcgtgacat tgagattcat
      481 gtcggttaac gatactggaa gttggttctt tttcataaac acatagattt tatcaaacaa
      541 atcgaactgc tgatccatca tcaataaggt cagtacacca taactgacag atgctccgtg
      601 aaggtatccc tccatttttt ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc
      661 agaattatag ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa tgatatccga
      721 aacgacttgt tcaattgcat atgagggacg atcgtcggca ctatctttca atgcttgcgt
      781 accataagtc aagagacgat caatgcacat ttttccgatt tggattccta atctatccgt
      841 gtagcctaga tcatcgccac gagcagaaaa agtagattcg acttctttgg ataaagcatc
      901 gccaatacct gcccataagt atttatcagg tgcttcggca ataattgctg tatcaatgaa
      961 ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc attacgcctt cttcgttata
     1021 gatgacacat accgcagtga cgggcgaaca gttcgaacca atagtgggaa aggtgaagac
     1081 tggtttagct agtttttcac caatgacttt tacagtatcg caagcacggc caccaccgac
     1141 tgcaaacagg acgtctgctt tttggacact ttcatgctgg atcaactgtt caatatttcc
     1201 ataagttgaa ttacccccgt accagagact ggcgacaacc tctatttcct gtgtcaaagc
     1261 tacttttatt ttgttttctg cctttgaaag tgcagtcttc cctccaatca gcactacttt
     1321 tgtcccatat tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata
     1381 atttggtaag aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt
     1441 aaaattttaa agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag
     1501 acaggataag tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa
     1561 aaacgaacaa gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat
     1621 ttctaccgat tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt
     1681 atatgaaaac aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat
     1741 ctatatcatg atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc
     1801 tattttgaat aaagtgtttc tgtataattt ttttatttta taagtaagat aaataatgaa
     1861 agaatagaga tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa
     1921 aaggactgtt agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac
     1981 cgtaataatc cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa
     2041 gaatatggag acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg
     2101 gtagcagcgg tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt
     2161 atcacaggtt gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata
     2221 ggtactcgat tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt
     2281 tatttaggta cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag
     2341 catacatttg ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat
     2401 gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg
     2461 gatatttgga tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt
     2521 gtgacagaag taatcagctt caagaaataa gaaggaattt acgaaaatta ctt
//
LOCUS       DAGCWU010000361         2007 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.245, whole genome shotgun sequence.
ACCESSION   DAGCWU010000361 DAGCWU010000000
VERSION     DAGCWU010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.245"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            76..1449
                     /gene="tet(L)"
                     /locus_tag="MBZ71_002901"
     CDS             76..1449
                     /gene="tet(L)"
                     /locus_tag="MBZ71_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBT4430764.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT          582 a          302 c          410 g          713 t
ORIGIN      
        1 atatggtttc ttgttaaata agatgaaata ttttttaata aagatttgaa ttaaagtgta
       61 aaggaggaat taattatgaa gtgtaatgaa tgtaacaggg ttaaatacaa ccaaatttta
      121 atttggcttt gcgttttatc tttttttagc gtattaaatg aaatggtttt gaacgtctca
      181 ttacctgata ttgcaaatga ttttaataaa ccacctgcga gtacaaactg ggtgaacaca
      241 gcctttatgt taaccttttc cattggaaca gctgtatatg gaaagctatc tgatcaatta
      301 ggcatcaaaa ggttactcct atttggaatt ataataaatt gtttcgggtc ggtaattggg
      361 tttgttggcc attctttctt ttccttactt attatggctc gttttattca aggggctggt
      421 gcagctgcat ttccagcact cgtaatggtt gtagttgcgc gctatattcc aaaggaaaat
      481 aggggtaaag catttggtct tattggatcg atagtagcca tgggagaagg agtcggtcca
      541 gcgattggtg gaatgatagc ccattatatt cattggtcct atcttctact cattcctatg
      601 ataacaatta tcactgttcc gtttcttatg aaattattaa agaaagaagt aaggataaaa
      661 ggtcattttg atatcaaagg aattatacta atgtctgtag gcattgtatt ttttatgttg
      721 tttacaacat catatagcat ttcttttctt atcgttagcg tgctgtcatt cctgatattt
      781 gtaaaacata tcaggaaagt aacagatcct tttgttgatc ccggattagg gaaaaatata
      841 ccttttatga ttggagttct ttgtggggga attatatttg gaacagtagc agggtttgtc
      901 tctatggttc cttatatgat gaaagatgtt caccagctaa gtactgccga aatcggaagt
      961 gtaattattt tccctggaac aatgagtgtc attattttcg gctacattgg tgggatactt
     1021 gttgatagaa gaggtccttt atacgtgtta aacatcggag ttacatttct ttctgttagc
     1081 tttttaactg cttcctttct tttagaaaca acatcatggt tcatgacaat tataatcgta
     1141 tttgttttag gtgggctttc gttcaccaaa acagttatat caacaattgt ttcaagtagc
     1201 ttgaaacagc aggaagctgg tgctggaatg agtttgctta actttaccag ctttttatca
     1261 gagggaacag gtattgcaat tgtaggtggt ttattatcca tacccttact tgatcaaagg
     1321 ttgttaccta tggaagttga tcagtcaact tatctgtata gtaatttgtt attacttttt
     1381 tcaggaatca ttgtcattag ttggctggtt accttgaatg tatataaaca ttctcaaagg
     1441 gatttctaaa tcgttaaggg atcaactttg ggagagagtt caaaattgat ccttttttta
     1501 taacaggaat tcaaatcttt ttgttccatt aaagggcgcg attgctgaat aaaagatacg
     1561 agagacctct cttgtatctt ttttattttg agtggttttg tccgttacac tagaaaaccg
     1621 aaagacaata aaaattttat tcttgctgag tctggctttc ggtaagctag acaaaacgga
     1681 caaaataaaa attggcaagg gtttaaaggt ggagattttt tgagtgatct tctcaaaaaa
     1741 tactacctgt cccttgctga tttttaaacg agcacgagag caaaaccccc ctttgctgag
     1801 gtggcagagg gcaggttttt ttgtttcttt tttctcgtaa aaaaaagaaa ggtcttaaag
     1861 gttttatggt tttggtcggc actgccgaca gcctcgcaga gcacacactt tatgaatata
     1921 aagtatagtg tgttatactt tacttggaag tggttgccgg aaagagcgaa aatgcctcac
     1981 atttgtgcca cctaaaaagg agcgatt
//
LOCUS       DAGCWU010000362         1934 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.247, whole genome shotgun sequence.
ACCESSION   DAGCWU010000362 DAGCWU010000000
VERSION     DAGCWU010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.247"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            22..>1934
                     /gene="tet(M)"
                     /locus_tag="MBZ71_002902"
     CDS             22..>1934
                     /gene="tet(M)"
                     /locus_tag="MBZ71_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBT4430765.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNK"
BASE COUNT          663 a          310 c          404 g          557 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaat
//
LOCUS       DAGCWU010000363         1934 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.248, whole genome shotgun sequence.
ACCESSION   DAGCWU010000363 DAGCWU010000000
VERSION     DAGCWU010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.248"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            22..>1934
                     /gene="tet(M)"
                     /locus_tag="MBZ71_002903"
     CDS             22..>1934
                     /gene="tet(M)"
                     /locus_tag="MBZ71_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBT4430766.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNK"
BASE COUNT          662 a          310 c          405 g          557 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaat
//
LOCUS       DAGCWU010000364         1166 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.296, whole genome shotgun sequence.
ACCESSION   DAGCWU010000364 DAGCWU010000000
VERSION     DAGCWU010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.296"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..167
                     /locus_tag="MBZ71_002904"
     CDS             <1..167
                     /locus_tag="MBZ71_002904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4430767.1"
                     /translation="LFDSRFKTLQQNPKWPLEDRNLADTALWLNAEFMKNAAEIGYAR
                     FLYGNSMKQK"
     gene            236..1147
                     /gene="aph(2'')"
                     /locus_tag="MBZ71_002905"
     CDS             236..1147
                     /gene="aph(2'')"
                     /locus_tag="MBZ71_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021401024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ie/IVa family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBT4430768.1"
                     /translation="MTTYTFDQVEEAIEQLYPDFTINTIEISGEGNDCIAYEINGNFI
                     FKFPKHSRASINLLNEVTVLKTIHNELSLPIPEVVFTGMPSEMCQMSFAGFTKIKGVP
                     LTPLLLKNLPKQSQDQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKL
                     LSRELKGHQMKKVDDFYRDILDNEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDF
                     GDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYMMKEKYWSFEKI
                     IYGKEYGYMDWYEEGLNEIRSSELLTT"
BASE COUNT          437 a          177 c          215 g          337 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc aaaatggcca ttagaagata
       61 ggaacttagc agatactgca ctttggctaa atgcagaatt tatgaaaaat gcagcagaga
      121 taggttatgc aagattttta tatggaaata gcatgaaaca aaaatgaatt aactataatt
      181 gctaaattct tatttacagt caatcagatt tttgattaaa gggagtagag aagacatgac
      241 aacttatact ttcgaccagg tagaagaggc aatagagcag ttatatcctg attttactat
      301 caatacaata gagatttcag gagaaggcaa tgactgtatt gcatatgaaa taaacgggaa
      361 ttttattttt aaatttccaa agcattcaag agcttcgatt aatctcttga atgaagtaac
      421 cgtactcaaa acaatccaca atgaattatc actacccatt cccgaggtgg tttttacagg
      481 aatgccatca gaaatgtgcc aaatgtcttt cgcaggtttt acaaaaatta aaggagtacc
      541 tttgacacct cttctactca aaaatctgcc gaagcaatct caagatcagg cagctaagga
      601 cctggcccga tttttaagtg aacttcacag cataaatatc tctggattca aaagtaatct
      661 ggtattagat tttcgagaga agataaatga agataataaa aaaatcaaaa agttactatc
      721 cagggaatta aagggtcacc agatgaagaa agtggatgat ttttacaggg atattctaga
      781 caacgaaatc tacttcaaat actatccttg tcttattcat aacgatttca gcagcgatca
      841 tattttattt gataccgaaa aaaataccat ttgtggaata atcgattttg gagatgcagc
      901 tatttctgat cccgacaatg attttataag tttgatggaa gatgatgaag agtacggcat
      961 ggaattcgta tcaaaaatat tgaaccatta caaacataag gatataccga cagttttgga
     1021 aaaatatatg atgaaagaaa aatactggtc gttcgaaaag attatctatg gaaaggaata
     1081 tggttatatg gattggtatg aagagggatt aaatgaaatc agaagcagtg aactactcac
     1141 cacttagcta aacctgacgg ttctta
//
LOCUS       DAGCWU010000365          932 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.305, whole genome shotgun sequence.
ACCESSION   DAGCWU010000365 DAGCWU010000000
VERSION     DAGCWU010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.305"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..56
                     /gene="ermCL"
                     /locus_tag="MBZ71_002906"
     CDS             <1..56
                     /gene="ermCL"
                     /locus_tag="MBZ71_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HBT4430769.1"
                     /translation="IFSIFVINTVHYQPNKK"
     gene            104..838
                     /locus_tag="MBZ71_002907"
     CDS             104..838
                     /locus_tag="MBZ71_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HBT4430770.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          381 a          126 c          124 g          301 t
ORIGIN      
        1 gcatttttag tatttttgta atcaacacag ttcattatca accaaacaaa aaataagtgg
       61 ttataatgga ttgttaatat aaacctcata ggagtgggtt ataatgaaca aaaaaaatat
      121 aaaagatagt caaaacttta ttacttcaaa gcatcatata aatgaaattt tgagaaatgt
      181 acatttaaat acaaatgata atattattga gattggttca gggaaaggtc atttcacgtt
      241 tgaattagct aaaaggtgta attatgtaac cgccattgaa atagatccta aattatgtag
      301 gataactaaa aacaaactta ttgaatatga gaacttccag gttatcaata aagatatttt
      361 acaatttaag tttcctaaaa ataagtcata taagatatat ggaaatatac cctacaatat
      421 aagtacagat ataattcgaa aaattgtttt tgaaagcaca gctacagaaa gttatttaat
      481 agtggaatat ggatttgcta aaaggttgct aaatacaaat cgttcactag cactattttt
      541 aatgacagaa gttgatatat ccatattaag taaaatccct agagaatact ttcatccaaa
      601 acctagagtt aatagctcgt taattgtatt aaaaagacac ccttcaaaaa tatcactcaa
      661 agatagaaaa caatatgaaa attttgttat gaaatgggtt aacaaagaat acataaaact
      721 attttccaaa aaccaatttt atcaagcctt aaaatatgca agaattgacg atttaaacaa
      781 tattagcttt gaacaattct tgtctctttt caatagctat aaattattta atagataagt
      841 taaggggtgc ataaactgca tcccttaact tatcttattt tgcttaaaca atcttggccc
      901 gtaaactgca taactcatat gtaaatcgct cc
//
LOCUS       DAGCWU010000366          794 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.315, whole genome shotgun sequence.
ACCESSION   DAGCWU010000366 DAGCWU010000000
VERSION     DAGCWU010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.315"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            69..>794
                     /gene="aph(2'')-I"
                     /locus_tag="MBZ71_002908"
     CDS             69..>794
                     /gene="aph(2'')-I"
                     /locus_tag="MBZ71_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBT4430771.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYG"
BASE COUNT          331 a           56 c          136 g          271 t
ORIGIN      
        1 agggcaaaaa agaagattgt tatttaatgg aatatagata tgatgataat gccacaaatg
       61 ttaaggcaat gaaatattta attgagcatt actttgataa tttcaaagta gatagtattg
      121 aaataatcgg tagtggttat gatagtgtgg catatttagt taataatgaa tacattttta
      181 aaacaaaatt tagtactaat aagaaaaaag gttatgcaaa agaaaaagca atatataatt
      241 ttttaaatac aaatttagaa actaatgtaa aaattcctaa tattgaatat tcgtatatta
      301 gtgatgaatt atctatacta ggttataaag aaattaaagg aactttttta acaccagaaa
      361 tttattctac tatgtcagaa gaagaacaaa atttgttaaa acgagatatt gccagttttt
      421 taagacaaat gcacggttta gattatacag atattagtga atgtactatt gataataaac
      481 aaaatgtatt agaagagtat atattgttgc gtgaaactat ttataatgat ttaactgata
      541 tagaaaaaga ttatatagaa agttttatgg aaagactaaa tgcaacaaca gtttttgagg
      601 gtaaaaagtg tttatgccat aatgatttta gttgtaatca tctattgtta gatggcaata
      661 atagattaac tggaataatt gattttggag attctggaat tatagatgaa tattgtgatt
      721 ttatatactt acttgaagat agtgaagaag aaataggaac aaattttgga gaagatatat
      781 taagaatgta tgga
//
LOCUS       DAGCWU010000367          514 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911619
            SAMN25126078-rid13883013.guided.343, whole genome shotgun sequence.
ACCESSION   DAGCWU010000367 DAGCWU010000000
VERSION     DAGCWU010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126078
            Sequence Read Archive: SRR17671396
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242183.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242183.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,788
            CDSs (with protein)               :: 2,788
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 28 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126078-rid13883013.guided.343"
                     /strain="M911619"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            6..>514
                     /gene="aac(6')-I"
                     /locus_tag="MBZ71_002909"
     CDS             6..>514
                     /gene="aac(6')-I"
                     /locus_tag="MBZ71_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HBT4430772.1"
                     /translation="MRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLESLKKHYTEP
                     WEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKG
                     IGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKK
                     EDCYLMEYR"
BASE COUNT          211 a           43 c           94 g          166 t
ORIGIN      
        1 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
       61 aaagagtatt agaattttat ggtggtagag ataaaaaata tacattagaa tcattaaaaa
      121 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      181 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      241 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      301 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      361 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      421 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      481 aaaaagaaga ttgttattta atggaatata gata
//
